Spg015827 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg015827
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionNADPH-dependent diflavin oxidoreductase 1
Locationscaffold6: 42378248 .. 42383467 (-)
RNA-Seq ExpressionSpg015827
SyntenySpg015827
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CGGATATTCGGTCCAATATAAAAAATTGGGATCCCATCCAAGAATGGGGGCATGTTGAGGTCGTGGCGACGTGCGAGCGGCGGCGCAAATTGTAGTGTTTCGGGTGGTTACACATATAATTTAACCTTTGTTTGGAAAAAAAAGAAAAAAAAAACGTAAACGGTTTATCTTCCTCTCACTCTCACGTATTCCACCTCCTTCCGCCGTCTCCTCCTCCGTTTCAGCAGCTCAGTGTCGCCGCCGCCTCCCTCCCGTTGAGTGCTGCTGCCGTCGTCTTTCTTCTGCTCGTGCCGCCGCAGCCTCCTCCCAGCGGTTTGACACTGATTTTTTAGTCTCTTGTTGTTACCGGTATTCTTCCAATCTCCATCTATGTCTTTCTCCTCACTTGAAAAACATAAATTAGGACTTGTCTAATGAAAGAACGGGGTTGTTTTGAATTTGCTGCATATGTCTGTCCATTGAGGATTTGATCATCTGTATTTGTTTCTGCAACCATAATTTTCCAGCATCTCCCTTCTTCTTTATTTATCGGAGAGTCAGATTCGAAGTAATCTATCGTACTATCCAATTCTCTTTTAATCCCTAGCTTGTTGGAGTTACTTTGATTGACCGAGGCATTCTAACGGTTCCCTGACCCTCCAACCCAGATTCTCCCAGCTCAATACCCTTCGTGGTTTGGGAAGAAGCCTAGCCTTGTTCTAGCCTTCTGTTTGGAGTTTCTGGAAGAAGTGCAGCACTTCAGCTTCAAAATCGTCTCTTGGGCAGTTGGTAACATGTAGTTAGTCTATTTCTCTTGGGTATGAGGATTTTTTGTTGGGAGGGGGAACCAGAGTTTTCATTCCCCTCTTTGAGCCATTTTTAGCTTGCATTTTTGGGATTTTCTAGTTGGCTGTTTTTGTGTAAAGCCTCTGTTTCGCGATCAATTTCCTTAGACTTATTTTTCAGATCCTTTTTCTTTGCCACCAAGACCCCAAACACAAACAGCCCTCGGTTCCACCCCTTGAAAGCTTCTCCCCAAGCTCTCTTCAGAAAATCTAGGTGTTGTAGCGTGTTCTCAAGCCTAAAAGAAGTTGGAACCTGTCTCAAAATTGTTGTTTATACTCTAAAGTTAACCCATTTTTACTTGTACCCTCGAGTCCTTAAACTTGTCAATCCAGTATCGTGGCAGCCTTTTCCCCAATTTTCTACGAATTTCTTGATCGTTTGGCTTTTCCTTTCTTAAAATTGTTTGACTTTGTTTTTCGGTGAAAGACATGAAAGACCAACCTGAGTTACTGATACTATATGCCACTCAAACTGGCAATGCACAAGATGCTGCAGAGAGGCTAGGCCGTGAAGCTGAGCATAGAGGTTGTGCTGTGAGGCTTCTTTCAATGGATGAGTATGATGCGGTAGGTTTTTCATCTTTTCCTTATTCCCTTTGTTTTGTTGCTAACGAAATGCTAGAATCATGAAATCATAACCAATTATATGTGTACTTGGTCTTCCAATTGTGGTGAATGGACAACATGATTTCCTTAAATCATTGTTTATCCACAAGGATTGATAACATTGTCTCAATCGTGATTTCCTGTAGTTTTTATTTTTATTTCTTCTTTTTTTTTTTTTTCTAATTTTTGAATAATGGAGGATTCTATTCTTTTTTCTAATTGTTAGAATAAGGTTTCAAATGATTGGATTGTATAGAGATTATAATTGATCTAGAAAAAGGCCAGGGGGGTTGTGGAGGACTGATTATTGTGGCTTATAAGGCAAGAAAAAATTGATTCAACGGAAGCTAGGGTCAATGATGACCAAAACTTGGAATTTATTTTTCTGGTTGTGAATGCATAATTGAAATAAGAGGGAAGGTGGCATTTCCATATTAAGCCTGTCTTGAGAGAGGAGACTACAGACAAGCAAATGAGTTATGTACTCTAAAAGCTTTTATAGGTGTTAAATTGTTTGAGCGATCTATGAAGGAAGATTTCGAAAATTAGCAGTTGAGATATCAGGGTTAGGATGTTTGAATTGATCTTTTTTCTTTTTTTTTTTTGGTTAAAAAAAGGTGGTTTGAACTGATCTATTCCTTTGAAATGGTTGAAGAAGATTATAAGGCTATGCCACATTTTGCAAAAGTCAATTTTGTAGGAAGTAGTTTGGATATTAGAAAGGATTCTTGTAGGTAGGACGGATGGTTTGCAAGCATAAATATGGAAATCAATATCCAAGATAATAAAACCATCAATAACAGTAACGTAAGCATGAGACCTTTTGGTAGTACCGGTGAGCTAGATGGAATCAATTTAATTATGAACAGGTAAAGAATGATGGTTTGAGTAAATGATGGATACTTCTCCTTGCTGTATGAATTATGATGTGGTAGCAAGATTCAAAAACTTGAATCTCAAAAACCTTTTATCCTTTGGATAAGTTACAATTAGCAGAACATGAGTCTAATTGGATTGCTTATTTTGTTTGAGAGTACCCATACTGGACGGTGGCCTACCCAATTATGAAGGAGTAAAGGTTGTCAAGCATCAAGGATAAAAGGCCTTAATCATTATTACCTAGAAAATTCAGTTGAAAGATCTTTGAAGGATAGAAGGAATTGTAGTAGGGGTGTGAAAGATCTTTAAAGGAATTGGGTAGAGGCCAGAGCTCTTAAAGAGTATGGAATTCTTTGTAGCTACTCTTGGGTGAAGGAATGCTCATTGTTGCCTAATCTTATGTTAATCATCATATCCTTCTTGATACAATTTTCTTTATTAGACACAATGTAAAAAATTCAAAATGGCAATTGGAAGGAACATTTCAATAAAGAAGTTGAATAAGATGAAATATTGGAATAAGAAGTTTCTTGAAAATGCACAATTACTCAATTAGCCTCACGGAGCCAAGGTCTCCTATCTTCATGACAGCCTGTTGCACTTGTTGGGAGTGAATGTTAAGGGATTGATGTTAAGGTAGTTTCAACTTTTATATCCACTCTTAATTTTACCCCTGCCCTTAGGCTGTTCCCTTTTGTGTTCTTTAAAGAAGTTCCTCTACAAGTTTCTTATATAAAAAGAAGTTCTTGGTACTGCATCTGGTCTTTTCCTCCTCTAAGTTATGTTTAATTTCTTTAAGAACGTTAACCCTTCTTTCACTATAATAGGAGTTTGTTTTGTGTTTGATATTGCTTTACATAACAGATATTTTGTATATCTTTGTACAGAGCAATACTTTGCCTGCTCCAGTAATTTTCTTTTTATCATCATGGTGTGTGAAGTAATCTGCACTTATCTATTTGTATTCAATGTTTCAGAGTTCTTTACCTCATGAAGATGCTATAATTTTTGTCGTCTCTACCACAGGCCAGGGTGAAACACCAGATTCCATGAGGGTTAGTGAGTTTTTATTTTTGGGGTACAGAAAATATTATTTCTATTATTTTGTGATTCATACTCCTCTCTCTCTCTCTCTCTCTCTCTTTTTGTGGCTGCCTGTTCCTCATTTTCTTTGTGGCAGGTCTTTTGGAGGTTCCTTCTACAAAGAAGTTTAAATCAGTATTGGCTCAAAGGAGTACCTTGTGCTGTATTTGGATTAGGTGATTCTGGTTATCAAAAATATAATGTAATATTTTTTCTGCTTTTCTTTGGAAGTAAAGCTAATTTATGCATTCCCCATTTTTTTCTACACTATGAAGTTCATTTCACTCGTTCTGCATCTTGGATGTGCTAGGCAAGGATTTGACAATTAGAAATTGATTGCGTTATAATTTTGAGTATTACATGTTAAGGTACCTGCTAATGTACGCTATTTATACAGTTTGTTGCAAAGAAGCTTGACAAAAGACTTTCTGATCTGGGAGCAAGGGCTATTCTGGAAAGAGGCCTGGGTGATGATCAACACCCATCAGGGTAGGCTTCCACTAATCTACTAATTTCTGGCTCAGTATCATAAAAGAGAATGTCTCAGCTAGGTGGGAATGATTCTTAGAAGAAGCTATTCAGCTTCTTTCCATTGGATCTACCTTCCCCTTAAGCAGATCCAGTCCTATGATCCTCAAAACAATAGGAAGTTTCATTTGGGATTGGTCTGATTTTGTTGGTGATAACATTACTATAGTTGTTTGAATGTTTTTGATGTCTTCCTTAGCTAGCATTTTCCGACGATAGAAAATAAAGTGAGCTGAAAGATCAATGCTGTCTGCCAGGTATGAGGCTGCTCTAGATCCTTGGATGTTATCATTGTGGAGGTCCTTGAATGATATTAATCCAATGTTCTTTCCAAAGGGCACTGATTTTGTGTTTTCAAATGACACGTTAATTGATCAACCAAGCATTCATGTTGCATATTACGATGCGGGCAAGTTGGATTCACAAATAACCTCAGGTATGCTAGTTTCTTGAATATTCATAATGAAGAATGCAATGGAACGACACATGATTTTTCTACCTTGTGGCCCTCTATCCTTGTTCGGTTAACATTCTATGTAAAATTGGAGAATTCAGATGTTGGCTTTGATGGGAATTTAGATTTGTGCTTCAAATAGAATGATATTCTAGCTGTATGTTTAGTTTATTCTATGAATGATATTCTTCAAATTGTTTACAAGATTGATGTGATGCGAATTTGTTTTTTGGTAAGCCTTCATACAATTTAATGACCAATTGACATTTCTTGTGTCTCTCAGATTTGAAGTATATGGAGATCCAAATTGAGAGGGCTCGCTCGATGTCTCCAGGAAAATTTTCTTTTGGCAAGAAAAAGCCTGATTGCTTTCTTAATATGGTATTAAACCTTGGCTAATTGTTATTGTTTCTTCTCAAATTCTGTTATCACTTAATATATAGAGATTTTGTTGTATATGGATGTGCTACAAGAGTTTTTAGCCATATCTCCTTATCATATTCTGAATATCTTCTGTTCTCAATTTTTTTTTTCTTCATGTAGATAAAAAATCAGCATTTGAGTAGAGTAGGCTCCGGAAAGGATGTGCGGCATTTTGAATTTGAATTTGTTTCTTCTGTAAGTTGGTAATGTCCACTTTTTCATTACTTATTTTATTTATTTGTTTTTTTTTTGTGACATAATGATTGCATTTTGAACTAACTTTAGGTAATAGAATATGAGGTTGGTGATGTCCTTGAGGTTCTTCCTAGTCAGAACCCTGCTGCAGTAAATGCTTTCATACAACGTTGCGATTTAGACCCAGAATCATTTATCACTGTAAGAGTTTGGTTTATAAGTTTGTAA

mRNA sequence

ATGAAAGACCAACCTGAGTTACTGATACTATATGCCACTCAAACTGGCAATGCACAAGATGCTGCAGAGAGGCTAGGCCGTGAAGCTGAGCATAGAGGTTGTGCTGTGAGGCTTCTTTCAATGGATGAGTATGATGCGAGTTCTTTACCTCATGAAGATGCTATAATTTTTGTCGTCTCTACCACAGGCCAGGGTGAAACACCAGATTCCATGAGGGTCTTTTGGAGGTTCCTTCTACAAAGAAGTTTAAATCAGTATTGGCTCAAAGGAGTACCTTGTGCTGTATTTGGATTAGGTGATTCTGGTTATCAAAAATATAATTTTGTTGCAAAGAAGCTTGACAAAAGACTTTCTGATCTGGGAGCAAGGGCTATTCTGGAAAGAGGCCTGGGTGATGATCAACACCCATCAGGGTATGAGGCTGCTCTAGATCCTTGGATGTTATCATTGTGGAGGTCCTTGAATGATATTAATCCAATGTTCTTTCCAAAGGGCACTGATTTTGTGTTTTCAAATGACACGTTAATTGATCAACCAAGCATTCATGTTGCATATTACGATGCGGGCAAGTTGGATTCACAAATAACCTCAGATTTGAAGTATATGGAGATCCAAATTGAGAGGGCTCGCTCGATGTCTCCAGGAAAATTTTCTTTTGGCAAGAAAAAGCCTGATTGCTTTCTTAATATGATAAAAAATCAGCATTTGAGTAGAGTAGGCTCCGGAAAGGATGTGCGGCATTTTGAATTTGAATTTGTTTCTTCTGTAATAGAATATGAGGTTGGTGATGTCCTTGAGGTTCTTCCTAGTCAGAACCCTGCTGCAGTAAATGCTTTCATACAACGTTGCGATTTAGACCCAGAATCATTTATCACTGTAAGAGTTTGGTTTATAAGTTTGTAA

Coding sequence (CDS)

ATGAAAGACCAACCTGAGTTACTGATACTATATGCCACTCAAACTGGCAATGCACAAGATGCTGCAGAGAGGCTAGGCCGTGAAGCTGAGCATAGAGGTTGTGCTGTGAGGCTTCTTTCAATGGATGAGTATGATGCGAGTTCTTTACCTCATGAAGATGCTATAATTTTTGTCGTCTCTACCACAGGCCAGGGTGAAACACCAGATTCCATGAGGGTCTTTTGGAGGTTCCTTCTACAAAGAAGTTTAAATCAGTATTGGCTCAAAGGAGTACCTTGTGCTGTATTTGGATTAGGTGATTCTGGTTATCAAAAATATAATTTTGTTGCAAAGAAGCTTGACAAAAGACTTTCTGATCTGGGAGCAAGGGCTATTCTGGAAAGAGGCCTGGGTGATGATCAACACCCATCAGGGTATGAGGCTGCTCTAGATCCTTGGATGTTATCATTGTGGAGGTCCTTGAATGATATTAATCCAATGTTCTTTCCAAAGGGCACTGATTTTGTGTTTTCAAATGACACGTTAATTGATCAACCAAGCATTCATGTTGCATATTACGATGCGGGCAAGTTGGATTCACAAATAACCTCAGATTTGAAGTATATGGAGATCCAAATTGAGAGGGCTCGCTCGATGTCTCCAGGAAAATTTTCTTTTGGCAAGAAAAAGCCTGATTGCTTTCTTAATATGATAAAAAATCAGCATTTGAGTAGAGTAGGCTCCGGAAAGGATGTGCGGCATTTTGAATTTGAATTTGTTTCTTCTGTAATAGAATATGAGGTTGGTGATGTCCTTGAGGTTCTTCCTAGTCAGAACCCTGCTGCAGTAAATGCTTTCATACAACGTTGCGATTTAGACCCAGAATCATTTATCACTGTAAGAGTTTGGTTTATAAGTTTGTAA

Protein sequence

MKDQPELLILYATQTGNAQDAAERLGREAEHRGCAVRLLSMDEYDASSLPHEDAIIFVVSTTGQGETPDSMRVFWRFLLQRSLNQYWLKGVPCAVFGLGDSGYQKYNFVAKKLDKRLSDLGARAILERGLGDDQHPSGYEAALDPWMLSLWRSLNDINPMFFPKGTDFVFSNDTLIDQPSIHVAYYDAGKLDSQITSDLKYMEIQIERARSMSPGKFSFGKKKPDCFLNMIKNQHLSRVGSGKDVRHFEFEFVSSVIEYEVGDVLEVLPSQNPAAVNAFIQRCDLDPESFITVRVWFISL
Homology
BLAST of Spg015827 vs. NCBI nr
Match: XP_022152796.1 (NADPH-dependent diflavin oxidoreductase 1 isoform X1 [Momordica charantia])

HSP 1 Score: 554.7 bits (1428), Expect = 5.0e-154
Identity = 269/293 (91.81%), Postives = 279/293 (95.22%), Query Frame = 0

Query: 1   MKDQPELLILYATQTGNAQDAAERLGREAEHRGCAVRLLSMDEYDASSLPHEDAIIFVVS 60
           MKDQPELLILYATQTGNAQDAAERLGREAEHRGC+VRLLSMDEYDASSLPHEDAIIFVVS
Sbjct: 1   MKDQPELLILYATQTGNAQDAAERLGREAEHRGCSVRLLSMDEYDASSLPHEDAIIFVVS 60

Query: 61  TTGQGETPDSMRVFWRFLLQRSLNQYWLKGVPCAVFGLGDSGYQKYNFVAKKLDKRLSDL 120
           TTGQGETPDSMRVFWR LLQRSL+QYWL+GVP AVFGLGDSGYQKYNFVAKKLDKRLSDL
Sbjct: 61  TTGQGETPDSMRVFWRSLLQRSLSQYWLEGVPYAVFGLGDSGYQKYNFVAKKLDKRLSDL 120

Query: 121 GARAILERGLGDDQHPSGYEAALDPWMLSLWRSLNDINPMFFPKGTDFVFSNDTLIDQPS 180
           GARAILERGLGDDQHPSGYEAALDPWMLSLWRSLNDI PMFFPKG DF FS+DTLIDQPS
Sbjct: 121 GARAILERGLGDDQHPSGYEAALDPWMLSLWRSLNDITPMFFPKGIDFAFSSDTLIDQPS 180

Query: 181 IHVAYYDAGKLDSQITSDLKYMEIQIERARSMSPGKFSFGKKKPDCFLNMIKNQHLSRVG 240
           IHV Y+D GKLD+Q+TSDLK MEIQIE AR MSPGK S GKKKPDCFLNMIKNQHL +VG
Sbjct: 181 IHVIYHDVGKLDTQLTSDLKRMEIQIESARLMSPGKCSLGKKKPDCFLNMIKNQHLCKVG 240

Query: 241 SGKDVRHFEFEFVSSVIEYEVGDVLEVLPSQNPAAVNAFIQRCDLDPESFITV 294
           SGKDVRHFEFEFVSSVIEYEVGDVLEVLPSQNPAAV+AFIQ C+LDPESFITV
Sbjct: 241 SGKDVRHFEFEFVSSVIEYEVGDVLEVLPSQNPAAVDAFIQHCNLDPESFITV 293

BLAST of Spg015827 vs. NCBI nr
Match: XP_038899117.1 (NADPH-dependent diflavin oxidoreductase 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 542.3 bits (1396), Expect = 2.5e-150
Identity = 264/293 (90.10%), Postives = 274/293 (93.52%), Query Frame = 0

Query: 1   MKDQPELLILYATQTGNAQDAAERLGREAEHRGCAVRLLSMDEYDASSLPHEDAIIFVVS 60
           MKDQPELLILYATQTGNA+DAAERLGREAEHRGC V+LLSMDEYDASSLPHEDAIIFVVS
Sbjct: 1   MKDQPELLILYATQTGNAEDAAERLGREAEHRGCVVKLLSMDEYDASSLPHEDAIIFVVS 60

Query: 61  TTGQGETPDSMRVFWRFLLQRSLNQYWLKGVPCAVFGLGDSGYQKYNFVAKKLDKRLSDL 120
           TTGQGETPDSMR FWRFLLQRSLNQYWLKGVP AVFGLGDS YQKYNFVAKKLDKRLSDL
Sbjct: 61  TTGQGETPDSMRAFWRFLLQRSLNQYWLKGVPYAVFGLGDSSYQKYNFVAKKLDKRLSDL 120

Query: 121 GARAILERGLGDDQHPSGYEAALDPWMLSLWRSLNDINPMFFPKGTDFVFSNDTLIDQPS 180
           GARAILERGLGDDQH SGYEAALDPWMLSLW SLNDINPMFF KGTDFV S+DT+IDQPS
Sbjct: 121 GARAILERGLGDDQHHSGYEAALDPWMLSLWSSLNDINPMFFLKGTDFVSSSDTIIDQPS 180

Query: 181 IHVAYYDAGKLDSQITSDLKYMEIQIERARSMSPGKFSFGKKKPDCFLNMIKNQHLSRVG 240
           I VAY + GKLDS + SDLKYME+QIERAR MSPGKFS GKKKPDCFL+MIKNQ LS+VG
Sbjct: 181 IQVAYCNVGKLDSPLNSDLKYMEMQIERARLMSPGKFSHGKKKPDCFLDMIKNQRLSKVG 240

Query: 241 SGKDVRHFEFEFVSSVIEYEVGDVLEVLPSQNPAAVNAFIQRCDLDPESFITV 294
           SGKDVRHFEFEFVSS IEYEVGDVLEVLPSQ  AAVNAFIQRCDLDPESFIT+
Sbjct: 241 SGKDVRHFEFEFVSSAIEYEVGDVLEVLPSQKSAAVNAFIQRCDLDPESFITI 293

BLAST of Spg015827 vs. NCBI nr
Match: XP_008453852.1 (PREDICTED: NADPH-dependent diflavin oxidoreductase 1 isoform X1 [Cucumis melo])

HSP 1 Score: 541.2 bits (1393), Expect = 5.7e-150
Identity = 265/293 (90.44%), Postives = 274/293 (93.52%), Query Frame = 0

Query: 1   MKDQPELLILYATQTGNAQDAAERLGREAEHRGCAVRLLSMDEYDASSLPHEDAIIFVVS 60
           MKDQPELLILYATQTGNAQDAAERLGREAEHRGC VRLLSMDEYDAS LPHED IIFVVS
Sbjct: 1   MKDQPELLILYATQTGNAQDAAERLGREAEHRGCVVRLLSMDEYDASHLPHEDGIIFVVS 60

Query: 61  TTGQGETPDSMRVFWRFLLQRSLNQYWLKGVPCAVFGLGDSGYQKYNFVAKKLDKRLSDL 120
           TTGQGETPDSMRVFW+FLLQRSL+QYWLKGVP AVFGLGDS YQKYNFVAKKLDKRLSDL
Sbjct: 61  TTGQGETPDSMRVFWKFLLQRSLDQYWLKGVPYAVFGLGDSSYQKYNFVAKKLDKRLSDL 120

Query: 121 GARAILERGLGDDQHPSGYEAALDPWMLSLWRSLNDINPMFFPKGTDFVFSNDTLIDQPS 180
           GA AILERGLGDDQH SGYEAALDPWMLSLW SLNDINPMFF KGTDFVFSNDT+IDQPS
Sbjct: 121 GATAILERGLGDDQHHSGYEAALDPWMLSLWSSLNDINPMFFLKGTDFVFSNDTIIDQPS 180

Query: 181 IHVAYYDAGKLDSQITSDLKYMEIQIERARSMSPGKFSFGKKKPDCFLNMIKNQHLSRVG 240
           + VAYY+ GKLDS +TSDLK  EIQIERAR MSPGKFS GKKKPDCFL MIKNQ LS+VG
Sbjct: 181 VQVAYYNMGKLDSPLTSDLKCTEIQIERARLMSPGKFSHGKKKPDCFLKMIKNQCLSKVG 240

Query: 241 SGKDVRHFEFEFVSSVIEYEVGDVLEVLPSQNPAAVNAFIQRCDLDPESFITV 294
           SGKDVRHFEFEFVSSVIEYEVGDVLEVLPSQ+ AAVNAFIQRC+LDPESFITV
Sbjct: 241 SGKDVRHFEFEFVSSVIEYEVGDVLEVLPSQSSAAVNAFIQRCNLDPESFITV 293

BLAST of Spg015827 vs. NCBI nr
Match: XP_011653099.1 (NADPH-dependent diflavin oxidoreductase 1 isoform X1 [Cucumis sativus] >KGN53174.1 hypothetical protein Csa_014898 [Cucumis sativus])

HSP 1 Score: 536.6 bits (1381), Expect = 1.4e-148
Identity = 264/293 (90.10%), Postives = 274/293 (93.52%), Query Frame = 0

Query: 1   MKDQPELLILYATQTGNAQDAAERLGREAEHRGCAVRLLSMDEYDASSLPHEDAIIFVVS 60
           MKDQPELLILYATQTGNAQDAAERLGREAEHRGC VRLLS+DEYDAS LPHED IIFVVS
Sbjct: 1   MKDQPELLILYATQTGNAQDAAERLGREAEHRGCVVRLLSVDEYDASHLPHEDGIIFVVS 60

Query: 61  TTGQGETPDSMRVFWRFLLQRSLNQYWLKGVPCAVFGLGDSGYQKYNFVAKKLDKRLSDL 120
           TTGQGETPDSMRVFW+FLLQRSL+QYWLKGVP AVFGLGDS YQKYNFVAKKLDKRLSDL
Sbjct: 61  TTGQGETPDSMRVFWKFLLQRSLDQYWLKGVPYAVFGLGDSSYQKYNFVAKKLDKRLSDL 120

Query: 121 GARAILERGLGDDQHPSGYEAALDPWMLSLWRSLNDINPMFFPKGTDFVFSNDTLIDQPS 180
           GA AIL RGLGDDQH SGYEAALDPWMLSLW SLNDINPMFF KGTDFVFS DT+IDQPS
Sbjct: 121 GAAAILGRGLGDDQHHSGYEAALDPWMLSLWSSLNDINPMFFLKGTDFVFSIDTIIDQPS 180

Query: 181 IHVAYYDAGKLDSQITSDLKYMEIQIERARSMSPGKFSFGKKKPDCFLNMIKNQHLSRVG 240
           + VAYY+ GKLDSQ+TSDLKYMEI IERAR MSPGKFS GKKKPDCFL MIKNQ LS+VG
Sbjct: 181 VQVAYYNVGKLDSQLTSDLKYMEI-IERARLMSPGKFSHGKKKPDCFLKMIKNQRLSKVG 240

Query: 241 SGKDVRHFEFEFVSSVIEYEVGDVLEVLPSQNPAAVNAFIQRCDLDPESFITV 294
           SGKDVRHFEFEFVSSVIEYEVGDVLEVLPSQ+ AAVNAFIQRC+LDPESFITV
Sbjct: 241 SGKDVRHFEFEFVSSVIEYEVGDVLEVLPSQSSAAVNAFIQRCNLDPESFITV 292

BLAST of Spg015827 vs. NCBI nr
Match: XP_022922572.1 (NADPH-dependent diflavin oxidoreductase 1 isoform X1 [Cucurbita moschata])

HSP 1 Score: 532.3 bits (1370), Expect = 2.6e-147
Identity = 261/293 (89.08%), Postives = 273/293 (93.17%), Query Frame = 0

Query: 1   MKDQPELLILYATQTGNAQDAAERLGREAEHRGCAVRLLSMDEYDASSLPHEDAIIFVVS 60
           MKDQPELLILYATQTGNAQDAAERLGREAE RGC VRLLSMDEYDASSLP EDA+IFVVS
Sbjct: 1   MKDQPELLILYATQTGNAQDAAERLGREAELRGCVVRLLSMDEYDASSLPREDAVIFVVS 60

Query: 61  TTGQGETPDSMRVFWRFLLQRSLNQYWLKGVPCAVFGLGDSGYQKYNFVAKKLDKRLSDL 120
           TTGQGETPDSMR FWRFLLQRSLNQ WLKGVP AVFGLGDS YQKYNFVAKKLDKRLSDL
Sbjct: 61  TTGQGETPDSMRGFWRFLLQRSLNQSWLKGVPYAVFGLGDSSYQKYNFVAKKLDKRLSDL 120

Query: 121 GARAILERGLGDDQHPSGYEAALDPWMLSLWRSLNDINPMFFPKGTDFVFSNDTLIDQPS 180
           GARAILERGLGDDQH SGYEAALDPWMLSLW SL+D NPMFFPKG DF+FS+DTLIDQ S
Sbjct: 121 GARAILERGLGDDQHHSGYEAALDPWMLSLWSSLSDTNPMFFPKGIDFMFSSDTLIDQSS 180

Query: 181 IHVAYYDAGKLDSQITSDLKYMEIQIERARSMSPGKFSFGKKKPDCFLNMIKNQHLSRVG 240
           I VAY++ GKL+SQ+TSDLK ME+QIERAR MSPGKFS GKKKPDC LNMIKNQ LS+VG
Sbjct: 181 IQVAYHNVGKLNSQLTSDLKNMEMQIERARLMSPGKFSHGKKKPDCLLNMIKNQRLSKVG 240

Query: 241 SGKDVRHFEFEFVSSVIEYEVGDVLEVLPSQNPAAVNAFIQRCDLDPESFITV 294
           SGKDVRHFEFEFVSSVIEYEVGDVLEVLPSQNPAAVN FIQRC+LDPESFIT+
Sbjct: 241 SGKDVRHFEFEFVSSVIEYEVGDVLEVLPSQNPAAVNDFIQRCNLDPESFITI 293

BLAST of Spg015827 vs. ExPASy Swiss-Prot
Match: Q6NPS8 (NADPH-dependent diflavin oxidoreductase 1 OS=Arabidopsis thaliana OX=3702 GN=ATR3 PE=1 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 5.0e-109
Identity = 190/291 (65.29%), Postives = 228/291 (78.35%), Query Frame = 0

Query: 4   QPELLILYATQTGNAQDAAERLGREAEHRGCAVRLLSMDEYDASSLP-HEDAIIFVVSTT 63
           Q +LL+LYA+QTGNA DAAER+GREAE RG    ++S DE+D SSLP HE+A++FVVSTT
Sbjct: 5   QRKLLVLYASQTGNALDAAERIGREAERRGLPASVVSTDEFDTSSLPHHEEAVVFVVSTT 64

Query: 64  GQGETPDSMRVFWRFLLQRSLNQYWLKGVPCAVFGLGDSGYQKYNFVAKKLDKRLSDLGA 123
           GQG++PDS + FWRFLLQR+L  YWL+ V  AVFGLGDSGYQKYNFVAKKLDKRLSDLGA
Sbjct: 65  GQGDSPDSFKAFWRFLLQRNLGNYWLQQVRYAVFGLGDSGYQKYNFVAKKLDKRLSDLGA 124

Query: 124 RAILERGLGDDQHPSGYEAALDPWMLSLWRSLNDINPMFFPKGTDFVFSNDTLIDQPSIH 183
             I+E+GLGDDQHPSGYE  LDPWMLSLWR+L  INP +FPKG D     D +ID+P   
Sbjct: 125 TTIIEKGLGDDQHPSGYEGTLDPWMLSLWRTLYQINPKYFPKGPDVKIPQDEVIDKPKYR 184

Query: 184 VAYYDAGKLDSQITSDLKYMEIQIERARSMSPGKFSFGKKKPDCFLNMIKNQHLSRVGSG 243
           + ++   KL+ ++ SD       I+RAR MSPGK    K KPDCFL M +N+ L++  S 
Sbjct: 185 ILFHKQEKLEPKLLSDSDI----IQRARGMSPGKLFKDKSKPDCFLKMTRNEVLTKAEST 244

Query: 244 KDVRHFEFEFVSSVIEYEVGDVLEVLPSQNPAAVNAFIQRCDLDPESFITV 294
           KDVRHFEF+FVSS IEYEVGDV+E+LPSQN + V+AFI+RC LDPESFITV
Sbjct: 245 KDVRHFEFQFVSSTIEYEVGDVVELLPSQNSSVVDAFIERCGLDPESFITV 291

BLAST of Spg015827 vs. ExPASy Swiss-Prot
Match: Q6PFP6 (NADPH-dependent diflavin oxidoreductase 1 OS=Danio rerio OX=7955 GN=ndor1 PE=2 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 3.1e-50
Identity = 108/287 (37.63%), Postives = 162/287 (56.45%), Query Frame = 0

Query: 7   LLILYATQTGNAQDAAERLGREAEHRGCAVRLLSMDEYDASSLPHEDAIIFVVSTTGQGE 66
           +L+LY +QTG AQD AER+GR+A+ R   V++ ++D Y+  +L  E  ++FV +TTGQG+
Sbjct: 6   VLVLYGSQTGTAQDTAERIGRQAQRRRLRVKVEALDTYNVVNLISESLVVFVCATTGQGD 65

Query: 67  TPDSMRVFWRFLLQRSLNQYWLKGVPCAVFGLGDSGYQKYNFVAKKLDKRLSDLGARAIL 126
            PD+M+ FWRFL ++SL    L  + CAV GLGDS Y K+NFVAKKL KRL  LGA  +L
Sbjct: 66  PPDNMKKFWRFLFRKSLPADSLSRLDCAVLGLGDSSYPKFNFVAKKLHKRLLQLGANMLL 125

Query: 127 ERGLGDDQHPSGYEAALDPWMLSLWRSLNDINPMFFPKGTDFVFSNDTLIDQPSIHVAYY 186
             GL DDQH  G +  +DPW+LS W+    + P   P G   +   D L   P  ++ ++
Sbjct: 126 PVGLADDQHDLGPDGVIDPWLLSFWQKTLSLYPP--PAGLAPLREEDKL---PPRYIFHF 185

Query: 187 DAGKLDSQITSDLKYMEIQIERARSMSPGKFSFGKKKPDCFLNMIKNQHLSRVGSGKDVR 246
              ++ +++T  L+     ++   S +P +             ++ NQ ++     +DVR
Sbjct: 186 -LSEVPNKLTEHLQ----TVDNKSSPTPLR--------PFPAPLVFNQRVTHTAHFQDVR 245

Query: 247 HFEFEFVSSVIEYEVGDVLEVLPSQNPAAVNAFIQRCDLDPESFITV 294
           H EF+   S IE+  GD + + P      V    Q   LDPES+ T+
Sbjct: 246 HIEFDITGSNIEFSAGDTVMMRPCNTSEDVEQLCQLLKLDPESYFTL 274

BLAST of Spg015827 vs. ExPASy Swiss-Prot
Match: A2AI05 (NADPH-dependent diflavin oxidoreductase 1 OS=Mus musculus OX=10090 GN=Ndor1 PE=2 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 3.8e-48
Identity = 111/290 (38.28%), Postives = 154/290 (53.10%), Query Frame = 0

Query: 5   PELLILYATQTGNAQDAAERLGREAEHRGCAVRLLSMDEYDASSLPHEDAIIFVVSTTGQ 64
           P+LL+L+ +QTG AQD AERLGREA  R    R+ ++D Y  ++L  E  +IFV +TTGQ
Sbjct: 4   PQLLVLFGSQTGTAQDEAERLGREARRRRLGCRVQALDSYSVANLIREPLVIFVCATTGQ 63

Query: 65  GETPDSMRVFWRFLLQRSLNQYWLKGVPCAVFGLGDSGYQKYNFVAKKLDKRLSDLGARA 124
           G+ PD+M+ FWRF+ ++SL    L  +  AV GLGDS Y K+NFVAKKL +RL  LG  A
Sbjct: 64  GDPPDNMKNFWRFIFRKSLPSSSLCQMDFAVLGLGDSSYAKFNFVAKKLHRRLLQLGGSA 123

Query: 125 ILERGLGDDQHPSGYEAALDPWMLSLWRSLNDINPMFFPKGTDFVFSNDTLIDQPSIHVA 184
           +L   LGDDQH  G +AA+DPW+  LW  +  + P+               +D P I   
Sbjct: 124 LLPPCLGDDQHELGPDAAIDPWVGDLWEKIMVMYPV--------------PLDIPEIPHG 183

Query: 185 YYDAGKLDSQITSDLKYMEIQIERARSMSPGKFSFGKKKPDCFLNMIKNQHLSRVGSGKD 244
                K   Q   ++    I  E     S    +   +       +I NQ ++     +D
Sbjct: 184 VPLPSKFIFQFLQEVP--SIGAEELNIASSAPQTPPSELQPFLAPVITNQRVTGPQHFQD 243

Query: 245 VRHFEFEFVSSVIEYEVGDVLEVLPSQNPAAVNAFIQRCDLDPESFITVR 295
           VR  EF+   S I +  GDV+ +LPS + A    F Q   LDP  F T++
Sbjct: 244 VRLIEFDITDSNISFAAGDVVFILPSNSEAHTQQFCQVLCLDPNQFFTLK 277

BLAST of Spg015827 vs. ExPASy Swiss-Prot
Match: Q9UHB4 (NADPH-dependent diflavin oxidoreductase 1 OS=Homo sapiens OX=9606 GN=NDOR1 PE=1 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 1.2e-46
Identity = 111/294 (37.76%), Postives = 158/294 (53.74%), Query Frame = 0

Query: 5   PELLILYATQTGNAQDAAERLGREAEHRGCAVRLLSMDEYDASSLPHEDAIIFVVSTTGQ 64
           P+LL+L+ +QTG AQD +ERLGREA  R    R+ ++D Y   +L +E  +IFV +TTGQ
Sbjct: 4   PQLLVLFGSQTGTAQDVSERLGREARRRRLGCRVQALDSYPVVNLINEPLVIFVCATTGQ 63

Query: 65  GETPDSMRVFWRFLLQRSLNQYWLKGVPCAVFGLGDSGYQKYNFVAKKLDKRLSDLGARA 124
           G+ PD+M+ FWRF+ +++L    L  +  AV GLGDS Y K+NFVAKKL +RL  LG  A
Sbjct: 64  GDPPDNMKNFWRFIFRKNLPSTALCQMDFAVLGLGDSSYAKFNFVAKKLHRRLLQLGGSA 123

Query: 125 ILERGLGDDQHPSGYEAALDPWMLSLWRSLNDINPMFFPKGTDFVFSNDTLIDQPSIHVA 184
           +L   LGDDQH  G +AA+DPW+  LW  +  + P   P G   +         P +   
Sbjct: 124 LLPVCLGDDQHELGPDAAVDPWLRDLWDRVLGLYPP--PPGLTEI--------PPGV--- 183

Query: 185 YYDAGKLDSQITSDLKYME----IQIERARSMSPGKFSFGKKKPDCFLNMIKNQHLSRVG 244
                 L S+ T  L +++       E  R   PG      +       MI NQ ++   
Sbjct: 184 -----PLPSKFT--LLFLQEAPSTGSEGQRVAHPGSQEPPSESKPFLAPMISNQRVTGPS 243

Query: 245 SGKDVRHFEFEFVSSVIEYEVGDVLEVLPSQNPAAVNAFIQRCDLDPESFITVR 295
             +DVR  EF+ + S I +  GDV+ + PS + A V  F Q   LDP+    ++
Sbjct: 244 HFQDVRLIEFDILGSGISFAAGDVVLIQPSNSAAHVQRFCQVLGLDPDQLFMLQ 277

BLAST of Spg015827 vs. ExPASy Swiss-Prot
Match: Q1JPJ0 (NADPH-dependent diflavin oxidoreductase 1 OS=Bos taurus OX=9913 GN=NDOR1 PE=2 SV=2)

HSP 1 Score: 185.7 bits (470), Expect = 7.9e-46
Identity = 111/295 (37.63%), Postives = 153/295 (51.86%), Query Frame = 0

Query: 7   LLILYATQTGNAQDAAERLGREAEHRGCAVRLLSMDEYDASSLPHEDAIIFVVSTTGQGE 66
           LL+L+ +QTG AQD +ERLGREA  R  + R+  +D Y   +L +E  +IFV +TTGQG+
Sbjct: 6   LLVLFGSQTGTAQDVSERLGREARRRQLSCRVEELDSYPVVNLINEPLVIFVCATTGQGD 65

Query: 67  TPDSMRVFWRFLLQRSLNQYWLKGVPCAVFGLGDSGYQKYNFVAKKLDKRLSDLGARAIL 126
            PD+M+ FWRF+ +RSL    L+ +  AV GLGDS Y K+NFVAKKL +RL  LG  A+L
Sbjct: 66  PPDNMKSFWRFIFRRSLPSTALRQMDFAVLGLGDSSYAKFNFVAKKLHRRLLQLGGSALL 125

Query: 127 ERGLGDDQHPSGYEAALDPWMLSLWRSLNDINPM-----FFPKGT--DFVFSNDTLIDQP 186
              LGDDQH  G +AA+DPW+  LW  +   +P+       P G      F+   L D P
Sbjct: 126 PVCLGDDQHELGPDAAIDPWLQDLWEKVLGPHPVPLNLDLSPPGVLWPSKFTLQFLKDTP 185

Query: 187 SIHVAYYDAGKLDSQITSDLKYMEIQIERARSMSPGKFSFGKKKPDCFLNMIKNQHLSRV 246
           S       A   D Q            E    ++P               M+ NQ ++  
Sbjct: 186 SSGPEELCAAGTDPQGPPS--------ELQPFLAP---------------MVSNQRVTGP 245

Query: 247 GSGKDVRHFEFEFVSSVIEYEVGDVLEVLPSQNPAAVNAFIQRCDLDPESFITVR 295
              +DVR  EF+   S I +  GD++ + P    + V  F Q   LDPE   T++
Sbjct: 246 SHFQDVRLIEFDISGSGISFAAGDLVLIQPENTASHVQQFCQALGLDPEQHFTLQ 277

BLAST of Spg015827 vs. ExPASy TrEMBL
Match: A0A6J1DIU4 (NADPH-dependent diflavin oxidoreductase 1 OS=Momordica charantia OX=3673 GN=LOC111020428 PE=3 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 2.4e-154
Identity = 269/293 (91.81%), Postives = 279/293 (95.22%), Query Frame = 0

Query: 1   MKDQPELLILYATQTGNAQDAAERLGREAEHRGCAVRLLSMDEYDASSLPHEDAIIFVVS 60
           MKDQPELLILYATQTGNAQDAAERLGREAEHRGC+VRLLSMDEYDASSLPHEDAIIFVVS
Sbjct: 1   MKDQPELLILYATQTGNAQDAAERLGREAEHRGCSVRLLSMDEYDASSLPHEDAIIFVVS 60

Query: 61  TTGQGETPDSMRVFWRFLLQRSLNQYWLKGVPCAVFGLGDSGYQKYNFVAKKLDKRLSDL 120
           TTGQGETPDSMRVFWR LLQRSL+QYWL+GVP AVFGLGDSGYQKYNFVAKKLDKRLSDL
Sbjct: 61  TTGQGETPDSMRVFWRSLLQRSLSQYWLEGVPYAVFGLGDSGYQKYNFVAKKLDKRLSDL 120

Query: 121 GARAILERGLGDDQHPSGYEAALDPWMLSLWRSLNDINPMFFPKGTDFVFSNDTLIDQPS 180
           GARAILERGLGDDQHPSGYEAALDPWMLSLWRSLNDI PMFFPKG DF FS+DTLIDQPS
Sbjct: 121 GARAILERGLGDDQHPSGYEAALDPWMLSLWRSLNDITPMFFPKGIDFAFSSDTLIDQPS 180

Query: 181 IHVAYYDAGKLDSQITSDLKYMEIQIERARSMSPGKFSFGKKKPDCFLNMIKNQHLSRVG 240
           IHV Y+D GKLD+Q+TSDLK MEIQIE AR MSPGK S GKKKPDCFLNMIKNQHL +VG
Sbjct: 181 IHVIYHDVGKLDTQLTSDLKRMEIQIESARLMSPGKCSLGKKKPDCFLNMIKNQHLCKVG 240

Query: 241 SGKDVRHFEFEFVSSVIEYEVGDVLEVLPSQNPAAVNAFIQRCDLDPESFITV 294
           SGKDVRHFEFEFVSSVIEYEVGDVLEVLPSQNPAAV+AFIQ C+LDPESFITV
Sbjct: 241 SGKDVRHFEFEFVSSVIEYEVGDVLEVLPSQNPAAVDAFIQHCNLDPESFITV 293

BLAST of Spg015827 vs. ExPASy TrEMBL
Match: A0A1S3BYF8 (NADPH-dependent diflavin oxidoreductase 1 OS=Cucumis melo OX=3656 GN=LOC103494455 PE=3 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 2.7e-150
Identity = 265/293 (90.44%), Postives = 274/293 (93.52%), Query Frame = 0

Query: 1   MKDQPELLILYATQTGNAQDAAERLGREAEHRGCAVRLLSMDEYDASSLPHEDAIIFVVS 60
           MKDQPELLILYATQTGNAQDAAERLGREAEHRGC VRLLSMDEYDAS LPHED IIFVVS
Sbjct: 1   MKDQPELLILYATQTGNAQDAAERLGREAEHRGCVVRLLSMDEYDASHLPHEDGIIFVVS 60

Query: 61  TTGQGETPDSMRVFWRFLLQRSLNQYWLKGVPCAVFGLGDSGYQKYNFVAKKLDKRLSDL 120
           TTGQGETPDSMRVFW+FLLQRSL+QYWLKGVP AVFGLGDS YQKYNFVAKKLDKRLSDL
Sbjct: 61  TTGQGETPDSMRVFWKFLLQRSLDQYWLKGVPYAVFGLGDSSYQKYNFVAKKLDKRLSDL 120

Query: 121 GARAILERGLGDDQHPSGYEAALDPWMLSLWRSLNDINPMFFPKGTDFVFSNDTLIDQPS 180
           GA AILERGLGDDQH SGYEAALDPWMLSLW SLNDINPMFF KGTDFVFSNDT+IDQPS
Sbjct: 121 GATAILERGLGDDQHHSGYEAALDPWMLSLWSSLNDINPMFFLKGTDFVFSNDTIIDQPS 180

Query: 181 IHVAYYDAGKLDSQITSDLKYMEIQIERARSMSPGKFSFGKKKPDCFLNMIKNQHLSRVG 240
           + VAYY+ GKLDS +TSDLK  EIQIERAR MSPGKFS GKKKPDCFL MIKNQ LS+VG
Sbjct: 181 VQVAYYNMGKLDSPLTSDLKCTEIQIERARLMSPGKFSHGKKKPDCFLKMIKNQCLSKVG 240

Query: 241 SGKDVRHFEFEFVSSVIEYEVGDVLEVLPSQNPAAVNAFIQRCDLDPESFITV 294
           SGKDVRHFEFEFVSSVIEYEVGDVLEVLPSQ+ AAVNAFIQRC+LDPESFITV
Sbjct: 241 SGKDVRHFEFEFVSSVIEYEVGDVLEVLPSQSSAAVNAFIQRCNLDPESFITV 293

BLAST of Spg015827 vs. ExPASy TrEMBL
Match: A0A0A0KWB7 (NADPH-dependent diflavin oxidoreductase 1 OS=Cucumis sativus OX=3659 GN=Csa_4G025070 PE=3 SV=1)

HSP 1 Score: 536.6 bits (1381), Expect = 6.8e-149
Identity = 264/293 (90.10%), Postives = 274/293 (93.52%), Query Frame = 0

Query: 1   MKDQPELLILYATQTGNAQDAAERLGREAEHRGCAVRLLSMDEYDASSLPHEDAIIFVVS 60
           MKDQPELLILYATQTGNAQDAAERLGREAEHRGC VRLLS+DEYDAS LPHED IIFVVS
Sbjct: 1   MKDQPELLILYATQTGNAQDAAERLGREAEHRGCVVRLLSVDEYDASHLPHEDGIIFVVS 60

Query: 61  TTGQGETPDSMRVFWRFLLQRSLNQYWLKGVPCAVFGLGDSGYQKYNFVAKKLDKRLSDL 120
           TTGQGETPDSMRVFW+FLLQRSL+QYWLKGVP AVFGLGDS YQKYNFVAKKLDKRLSDL
Sbjct: 61  TTGQGETPDSMRVFWKFLLQRSLDQYWLKGVPYAVFGLGDSSYQKYNFVAKKLDKRLSDL 120

Query: 121 GARAILERGLGDDQHPSGYEAALDPWMLSLWRSLNDINPMFFPKGTDFVFSNDTLIDQPS 180
           GA AIL RGLGDDQH SGYEAALDPWMLSLW SLNDINPMFF KGTDFVFS DT+IDQPS
Sbjct: 121 GAAAILGRGLGDDQHHSGYEAALDPWMLSLWSSLNDINPMFFLKGTDFVFSIDTIIDQPS 180

Query: 181 IHVAYYDAGKLDSQITSDLKYMEIQIERARSMSPGKFSFGKKKPDCFLNMIKNQHLSRVG 240
           + VAYY+ GKLDSQ+TSDLKYMEI IERAR MSPGKFS GKKKPDCFL MIKNQ LS+VG
Sbjct: 181 VQVAYYNVGKLDSQLTSDLKYMEI-IERARLMSPGKFSHGKKKPDCFLKMIKNQRLSKVG 240

Query: 241 SGKDVRHFEFEFVSSVIEYEVGDVLEVLPSQNPAAVNAFIQRCDLDPESFITV 294
           SGKDVRHFEFEFVSSVIEYEVGDVLEVLPSQ+ AAVNAFIQRC+LDPESFITV
Sbjct: 241 SGKDVRHFEFEFVSSVIEYEVGDVLEVLPSQSSAAVNAFIQRCNLDPESFITV 292

BLAST of Spg015827 vs. ExPASy TrEMBL
Match: A0A6J1E3N5 (NADPH-dependent diflavin oxidoreductase 1 OS=Cucurbita moschata OX=3662 GN=LOC111430541 PE=3 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 1.3e-147
Identity = 261/293 (89.08%), Postives = 273/293 (93.17%), Query Frame = 0

Query: 1   MKDQPELLILYATQTGNAQDAAERLGREAEHRGCAVRLLSMDEYDASSLPHEDAIIFVVS 60
           MKDQPELLILYATQTGNAQDAAERLGREAE RGC VRLLSMDEYDASSLP EDA+IFVVS
Sbjct: 1   MKDQPELLILYATQTGNAQDAAERLGREAELRGCVVRLLSMDEYDASSLPREDAVIFVVS 60

Query: 61  TTGQGETPDSMRVFWRFLLQRSLNQYWLKGVPCAVFGLGDSGYQKYNFVAKKLDKRLSDL 120
           TTGQGETPDSMR FWRFLLQRSLNQ WLKGVP AVFGLGDS YQKYNFVAKKLDKRLSDL
Sbjct: 61  TTGQGETPDSMRGFWRFLLQRSLNQSWLKGVPYAVFGLGDSSYQKYNFVAKKLDKRLSDL 120

Query: 121 GARAILERGLGDDQHPSGYEAALDPWMLSLWRSLNDINPMFFPKGTDFVFSNDTLIDQPS 180
           GARAILERGLGDDQH SGYEAALDPWMLSLW SL+D NPMFFPKG DF+FS+DTLIDQ S
Sbjct: 121 GARAILERGLGDDQHHSGYEAALDPWMLSLWSSLSDTNPMFFPKGIDFMFSSDTLIDQSS 180

Query: 181 IHVAYYDAGKLDSQITSDLKYMEIQIERARSMSPGKFSFGKKKPDCFLNMIKNQHLSRVG 240
           I VAY++ GKL+SQ+TSDLK ME+QIERAR MSPGKFS GKKKPDC LNMIKNQ LS+VG
Sbjct: 181 IQVAYHNVGKLNSQLTSDLKNMEMQIERARLMSPGKFSHGKKKPDCLLNMIKNQRLSKVG 240

Query: 241 SGKDVRHFEFEFVSSVIEYEVGDVLEVLPSQNPAAVNAFIQRCDLDPESFITV 294
           SGKDVRHFEFEFVSSVIEYEVGDVLEVLPSQNPAAVN FIQRC+LDPESFIT+
Sbjct: 241 SGKDVRHFEFEFVSSVIEYEVGDVLEVLPSQNPAAVNDFIQRCNLDPESFITI 293

BLAST of Spg015827 vs. ExPASy TrEMBL
Match: A0A6J1JDP8 (NADPH-dependent diflavin oxidoreductase 1 OS=Cucurbita maxima OX=3661 GN=LOC111483472 PE=3 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 2.2e-147
Identity = 260/293 (88.74%), Postives = 273/293 (93.17%), Query Frame = 0

Query: 1   MKDQPELLILYATQTGNAQDAAERLGREAEHRGCAVRLLSMDEYDASSLPHEDAIIFVVS 60
           MKDQPELLILYATQTGNAQDAAERLGREAE RGC VRLLSMDEYDASSLP EDA+IFVVS
Sbjct: 1   MKDQPELLILYATQTGNAQDAAERLGREAELRGCVVRLLSMDEYDASSLPREDAVIFVVS 60

Query: 61  TTGQGETPDSMRVFWRFLLQRSLNQYWLKGVPCAVFGLGDSGYQKYNFVAKKLDKRLSDL 120
           TTGQGETPDSMR FWRFLLQRSLNQ WLKGVP AVFGLGDS YQKYNFVAKKLDKRLSDL
Sbjct: 61  TTGQGETPDSMRGFWRFLLQRSLNQSWLKGVPYAVFGLGDSSYQKYNFVAKKLDKRLSDL 120

Query: 121 GARAILERGLGDDQHPSGYEAALDPWMLSLWRSLNDINPMFFPKGTDFVFSNDTLIDQPS 180
           GARAILERGLGDDQH SGYEAALDPWMLSLW SL+D NPMFFPKG DF+FS+DTLIDQ S
Sbjct: 121 GARAILERGLGDDQHHSGYEAALDPWMLSLWSSLSDTNPMFFPKGIDFMFSSDTLIDQSS 180

Query: 181 IHVAYYDAGKLDSQITSDLKYMEIQIERARSMSPGKFSFGKKKPDCFLNMIKNQHLSRVG 240
           I VAY++ GK++SQ+TSDLK ME+QIERAR MSPGKFS GKKKPDC LNMIKNQ LS+VG
Sbjct: 181 IQVAYHNVGKMNSQLTSDLKNMEMQIERARLMSPGKFSHGKKKPDCLLNMIKNQRLSKVG 240

Query: 241 SGKDVRHFEFEFVSSVIEYEVGDVLEVLPSQNPAAVNAFIQRCDLDPESFITV 294
           SGKDVRHFEFEFVSSVIEYEVGDVLEVLPSQNPAAVN FIQRC+LDPESFIT+
Sbjct: 241 SGKDVRHFEFEFVSSVIEYEVGDVLEVLPSQNPAAVNDFIQRCNLDPESFITI 293

BLAST of Spg015827 vs. TAIR 10
Match: AT3G02280.1 (Flavodoxin family protein )

HSP 1 Score: 395.6 bits (1015), Expect = 3.6e-110
Identity = 190/291 (65.29%), Postives = 228/291 (78.35%), Query Frame = 0

Query: 4   QPELLILYATQTGNAQDAAERLGREAEHRGCAVRLLSMDEYDASSLP-HEDAIIFVVSTT 63
           Q +LL+LYA+QTGNA DAAER+GREAE RG    ++S DE+D SSLP HE+A++FVVSTT
Sbjct: 5   QRKLLVLYASQTGNALDAAERIGREAERRGLPASVVSTDEFDTSSLPHHEEAVVFVVSTT 64

Query: 64  GQGETPDSMRVFWRFLLQRSLNQYWLKGVPCAVFGLGDSGYQKYNFVAKKLDKRLSDLGA 123
           GQG++PDS + FWRFLLQR+L  YWL+ V  AVFGLGDSGYQKYNFVAKKLDKRLSDLGA
Sbjct: 65  GQGDSPDSFKAFWRFLLQRNLGNYWLQQVRYAVFGLGDSGYQKYNFVAKKLDKRLSDLGA 124

Query: 124 RAILERGLGDDQHPSGYEAALDPWMLSLWRSLNDINPMFFPKGTDFVFSNDTLIDQPSIH 183
             I+E+GLGDDQHPSGYE  LDPWMLSLWR+L  INP +FPKG D     D +ID+P   
Sbjct: 125 TTIIEKGLGDDQHPSGYEGTLDPWMLSLWRTLYQINPKYFPKGPDVKIPQDEVIDKPKYR 184

Query: 184 VAYYDAGKLDSQITSDLKYMEIQIERARSMSPGKFSFGKKKPDCFLNMIKNQHLSRVGSG 243
           + ++   KL+ ++ SD       I+RAR MSPGK    K KPDCFL M +N+ L++  S 
Sbjct: 185 ILFHKQEKLEPKLLSDSDI----IQRARGMSPGKLFKDKSKPDCFLKMTRNEVLTKAEST 244

Query: 244 KDVRHFEFEFVSSVIEYEVGDVLEVLPSQNPAAVNAFIQRCDLDPESFITV 294
           KDVRHFEF+FVSS IEYEVGDV+E+LPSQN + V+AFI+RC LDPESFITV
Sbjct: 245 KDVRHFEFQFVSSTIEYEVGDVVELLPSQNSSVVDAFIERCGLDPESFITV 291

BLAST of Spg015827 vs. TAIR 10
Match: AT4G30210.1 (P450 reductase 2 )

HSP 1 Score: 109.8 bits (273), Expect = 3.9e-24
Identity = 81/300 (27.00%), Postives = 136/300 (45.33%), Query Frame = 0

Query: 6   ELLILYATQTGNAQDAAERLGREAEHR--GCAVRLLSMDEYDA------SSLPHEDAIIF 65
           ++ I + TQTG A+  A+ LG EA+ R      +++ +D+Y A        L  ED   F
Sbjct: 104 KVTIFFGTQTGTAEGFAKALGEEAKARYEKTRFKIVDLDDYAADDDEYEEKLKKEDVAFF 163

Query: 66  VVSTTGQGETPDSMRVFWRFLLQRSLNQYWLKGVPCAVFGLGDSGYQKYNFVAKKLDKRL 125
            ++T G GE  D+   F+++  + +    WLK +   VFGLG+  Y+ +N VAK +D  L
Sbjct: 164 FLATYGDGEPTDNAARFYKWFTEGNDRGEWLKNLKYGVFGLGNRQYEHFNKVAKVVDDIL 223

Query: 126 SDLGARAILERGLGDDQHPSGYEAALDPWMLSLWRSLNDINPMFFPKGTDFVFSNDTLID 185
            + GA+ +++ GLGDD      E     W  +LW  L+ I               DT + 
Sbjct: 224 VEQGAQRLVQVGLGDDD--QCIEDDFTAWREALWPELDTI----------LREEGDTAVA 283

Query: 186 QPSIHVAYYDAGKLDSQI----TSDLKYMEIQIERARSMSPGKFSFGKKKPDCFLNMIKN 245
            P      Y A  L+ ++    + D K+ +I +        G   F  + P    N+   
Sbjct: 284 TP------YTAAVLEYRVSIHDSEDAKFNDINMANGN----GYTVFDAQHP-YKANVAVK 343

Query: 246 QHLSRVGSGKDVRHFEFEFVSSVIEYEVGDVLEVLPSQNPAAVNAFIQRCDLDPESFITV 294
           + L    S +   H EF+   S + YE GD + VL       V+  ++  D+ P+++ ++
Sbjct: 344 RELHTPESDRSCIHLEFDIAGSGLTYETGDHVGVLCDNLSETVDEALRLLDMSPDTYFSL 380

BLAST of Spg015827 vs. TAIR 10
Match: AT4G30210.2 (P450 reductase 2 )

HSP 1 Score: 109.8 bits (273), Expect = 3.9e-24
Identity = 81/300 (27.00%), Postives = 136/300 (45.33%), Query Frame = 0

Query: 6   ELLILYATQTGNAQDAAERLGREAEHR--GCAVRLLSMDEYDA------SSLPHEDAIIF 65
           ++ I + TQTG A+  A+ LG EA+ R      +++ +D+Y A        L  ED   F
Sbjct: 104 KVTIFFGTQTGTAEGFAKALGEEAKARYEKTRFKIVDLDDYAADDDEYEEKLKKEDVAFF 163

Query: 66  VVSTTGQGETPDSMRVFWRFLLQRSLNQYWLKGVPCAVFGLGDSGYQKYNFVAKKLDKRL 125
            ++T G GE  D+   F+++  + +    WLK +   VFGLG+  Y+ +N VAK +D  L
Sbjct: 164 FLATYGDGEPTDNAARFYKWFTEGNDRGEWLKNLKYGVFGLGNRQYEHFNKVAKVVDDIL 223

Query: 126 SDLGARAILERGLGDDQHPSGYEAALDPWMLSLWRSLNDINPMFFPKGTDFVFSNDTLID 185
            + GA+ +++ GLGDD      E     W  +LW  L+ I               DT + 
Sbjct: 224 VEQGAQRLVQVGLGDDD--QCIEDDFTAWREALWPELDTI----------LREEGDTAVA 283

Query: 186 QPSIHVAYYDAGKLDSQI----TSDLKYMEIQIERARSMSPGKFSFGKKKPDCFLNMIKN 245
            P      Y A  L+ ++    + D K+ +I +        G   F  + P    N+   
Sbjct: 284 TP------YTAAVLEYRVSIHDSEDAKFNDINMANGN----GYTVFDAQHP-YKANVAVK 343

Query: 246 QHLSRVGSGKDVRHFEFEFVSSVIEYEVGDVLEVLPSQNPAAVNAFIQRCDLDPESFITV 294
           + L    S +   H EF+   S + YE GD + VL       V+  ++  D+ P+++ ++
Sbjct: 344 RELHTPESDRSCIHLEFDIAGSGLTYETGDHVGVLCDNLSETVDEALRLLDMSPDTYFSL 380

BLAST of Spg015827 vs. TAIR 10
Match: AT4G24520.1 (P450 reductase 1 )

HSP 1 Score: 89.4 bits (220), Expect = 5.5e-18
Identity = 68/263 (25.86%), Postives = 116/263 (44.11%), Query Frame = 0

Query: 9   ILYATQTGNAQDAAERLGRE--AEHRGCAVRLLSMDEYDASSLPHEDAI------IFVVS 68
           I + TQTG A+  A+ L  E  A +   AV+++ +D+Y A    +E+ +       F V+
Sbjct: 87  IFFGTQTGTAEGFAKALSEEIKARYEKAAVKVIDLDDYAADDDQYEEKLKKETLAFFCVA 146

Query: 69  TTGQGETPDSMRVFWRFLLQRSLNQYWLKGVPCAVFGLGDSGYQKYNFVAKKLDKRLSDL 128
           T G GE  D+   F+++  + +     L+ +   VF LG+  Y+ +N +   LD+ L   
Sbjct: 147 TYGDGEPTDNAARFYKWFTEENERDIKLQQLAYGVFALGNRQYEHFNKIGIVLDEELCKK 206

Query: 129 GARAILERGLGDDQHPSGYEAALDPWMLSLWRSLNDINPMFFPKGTDFVFSNDTLIDQPS 188
           GA+ ++E GLGDD      E   + W  SLW  L            D +  ++   D  S
Sbjct: 207 GAKRLIEVGLGDDD--QSIEDDFNAWKESLWSEL------------DKLLKDE---DDKS 266

Query: 189 IHVAYYDAGKLDSQITSDLKYMEIQIERARSMSPGKFSFGKKKPDCFLNMIKNQHLSRVG 248
           +   Y         +T D ++   Q     +++ G  +     P C +++   + L    
Sbjct: 267 VATPYTAVIPEYRVVTHDPRF-TTQKSMESNVANGNTTIDIHHP-CRVDVAVQKELHTHE 326

Query: 249 SGKDVRHFEFEFVSSVIEYEVGD 264
           S +   H EF+   + I YE GD
Sbjct: 327 SDRSCIHLEFDISRTGITYETGD 330

BLAST of Spg015827 vs. TAIR 10
Match: AT4G24520.2 (P450 reductase 1 )

HSP 1 Score: 81.6 bits (200), Expect = 1.1e-15
Identity = 67/259 (25.87%), Postives = 110/259 (42.47%), Query Frame = 0

Query: 9   ILYATQTGNAQDAAERLGRE--AEHRGCAVR--LLSMDEYDASSLPHEDAIIFVVSTTGQ 68
           I + TQTG A+  A+ L  E  A +   AV+    + D+     L  E    F V+T G 
Sbjct: 87  IFFGTQTGTAEGFAKALSEEIKARYEKAAVKDDYAADDDQYEEKLKKETLAFFCVATYGD 146

Query: 69  GETPDSMRVFWRFLLQRSLNQYWLKGVPCAVFGLGDSGYQKYNFVAKKLDKRLSDLGARA 128
           GE  D+   F+++  + +     L+ +   VF LG+  Y+ +N +   LD+ L   GA+ 
Sbjct: 147 GEPTDNAARFYKWFTEENERDIKLQQLAYGVFALGNRQYEHFNKIGIVLDEELCKKGAKR 206

Query: 129 ILERGLGDDQHPSGYEAALDPWMLSLWRSLNDINPMFFPKGTDFVFSNDTLIDQPSIHVA 188
           ++E GLGDD      E   + W  SLW  L            D +  ++   D  S+   
Sbjct: 207 LIEVGLGDDD--QSIEDDFNAWKESLWSEL------------DKLLKDE---DDKSVATP 266

Query: 189 YYDAGKLDSQITSDLKYMEIQIERARSMSPGKFSFGKKKPDCFLNMIKNQHLSRVGSGKD 248
           Y         +T D ++   Q     +++ G  +     P C +++   + L    S + 
Sbjct: 267 YTAVIPEYRVVTHDPRF-TTQKSMESNVANGNTTIDIHHP-CRVDVAVQKELHTHESDRS 326

Query: 249 VRHFEFEFVSSVIEYEVGD 264
             H EF+   + I YE GD
Sbjct: 327 CIHLEFDISRTGITYETGD 326

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022152796.15.0e-15491.81NADPH-dependent diflavin oxidoreductase 1 isoform X1 [Momordica charantia][more]
XP_038899117.12.5e-15090.10NADPH-dependent diflavin oxidoreductase 1 isoform X1 [Benincasa hispida][more]
XP_008453852.15.7e-15090.44PREDICTED: NADPH-dependent diflavin oxidoreductase 1 isoform X1 [Cucumis melo][more]
XP_011653099.11.4e-14890.10NADPH-dependent diflavin oxidoreductase 1 isoform X1 [Cucumis sativus] >KGN53174... [more]
XP_022922572.12.6e-14789.08NADPH-dependent diflavin oxidoreductase 1 isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q6NPS85.0e-10965.29NADPH-dependent diflavin oxidoreductase 1 OS=Arabidopsis thaliana OX=3702 GN=ATR... [more]
Q6PFP63.1e-5037.63NADPH-dependent diflavin oxidoreductase 1 OS=Danio rerio OX=7955 GN=ndor1 PE=2 S... [more]
A2AI053.8e-4838.28NADPH-dependent diflavin oxidoreductase 1 OS=Mus musculus OX=10090 GN=Ndor1 PE=2... [more]
Q9UHB41.2e-4637.76NADPH-dependent diflavin oxidoreductase 1 OS=Homo sapiens OX=9606 GN=NDOR1 PE=1 ... [more]
Q1JPJ07.9e-4637.63NADPH-dependent diflavin oxidoreductase 1 OS=Bos taurus OX=9913 GN=NDOR1 PE=2 SV... [more]
Match NameE-valueIdentityDescription
A0A6J1DIU42.4e-15491.81NADPH-dependent diflavin oxidoreductase 1 OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A1S3BYF82.7e-15090.44NADPH-dependent diflavin oxidoreductase 1 OS=Cucumis melo OX=3656 GN=LOC10349445... [more]
A0A0A0KWB76.8e-14990.10NADPH-dependent diflavin oxidoreductase 1 OS=Cucumis sativus OX=3659 GN=Csa_4G02... [more]
A0A6J1E3N51.3e-14789.08NADPH-dependent diflavin oxidoreductase 1 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1JDP82.2e-14788.74NADPH-dependent diflavin oxidoreductase 1 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
AT3G02280.13.6e-11065.29Flavodoxin family protein [more]
AT4G30210.13.9e-2427.00P450 reductase 2 [more]
AT4G30210.23.9e-2427.00P450 reductase 2 [more]
AT4G24520.15.5e-1825.86P450 reductase 1 [more]
AT4G24520.21.1e-1525.87P450 reductase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001094Flavodoxin-likePRINTSPR00369FLAVODOXINcoord: 8..21
score: 42.97
coord: 90..100
score: 53.41
coord: 56..67
score: 42.32
coord: 114..133
score: 44.45
IPR023173NADPH-cytochrome p450 reductase, FAD-binding, alpha-helical domain superfamilyGENE3D1.20.990.10coord: 269..293
e-value: 4.4E-13
score: 51.4
NoneNo IPR availableGENE3D2.40.30.10Translation factorscoord: 229..268
e-value: 4.4E-13
score: 51.4
NoneNo IPR availablePANTHERPTHR19384:SF113SUBFAMILY NOT NAMEDcoord: 6..297
NoneNo IPR availablePANTHERPTHR19384NITRIC OXIDE SYNTHASE-RELATEDcoord: 6..297
IPR008254Flavodoxin/nitric oxide synthasePFAMPF00258Flavodoxin_1coord: 9..146
e-value: 1.3E-37
score: 129.1
IPR008254Flavodoxin/nitric oxide synthasePROSITEPS50902FLAVODOXIN_LIKEcoord: 7..151
score: 33.498577
IPR029039Flavoprotein-like superfamilyGENE3D3.40.50.360coord: 1..161
e-value: 2.2E-60
score: 204.9
IPR029039Flavoprotein-like superfamilySUPERFAMILY52218Flavoproteinscoord: 6..158
IPR003097Sulfite reductase [NADPH] flavoprotein alpha-component-like, FAD-bindingPFAMPF00667FAD_binding_1coord: 225..293
e-value: 4.5E-8
score: 32.9
IPR017938Riboflavin synthase-like beta-barrelSUPERFAMILY63380Riboflavin synthase domain-likecoord: 220..295

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg015827.1Spg015827.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016226 iron-sulfur cluster assembly
cellular_component GO:0005829 cytosol
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0010181 FMN binding
molecular_function GO:0050661 NADP binding
molecular_function GO:0003958 NADPH-hemoprotein reductase activity
molecular_function GO:0016491 oxidoreductase activity