Spg015789 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg015789
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionDelta 9 desaturase
Locationscaffold6: 43583578 .. 43585785 (+)
RNA-Seq ExpressionSpg015789
SyntenySpg015789
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGAAAACAATTGCTAGAAGCACTTCGTTATGGAAGCACCAAGAGAAGTAGAAAAGAAGGTGGCAGGGTCTCGCAGGGCCCTTTGGAGGAGAAAATGGACATTTTTGGATATACGTTCTGCTACCTTCATCTTATTAGCACATTTACTTTGTATTTTTGCACCCTTTCATTTTAGTTGGGCTGCCCTTTTGGTTGCCTTTACACTTTACGTGGTCGTTTCGGTTTTCGGGATGGCACTCTCGTATCATAGAAATCTCACGCATAAGAGTTTCAAACTTCCTAAATGGCTGGAGTACCTGTTTGCGTATGTGGGAGTAATGGCACTTCAGGTTTGTGTAAAAAAGAGCAGAAAAAGAACTTTGTGTTAGAATAACTTTCTTTTTTTGGAAAAATTACCTCTTTGGTCCCTGAATTTTGAGGAATATGTGCGTTTAGTCCCTGAGTTTTCAAAATAGACCTTTTTAGTTCATGAATTTTGAGAAATAGGTTTAAAAGGTCCCTAAGTTTTTAAAATAGACATATTTAGTCAATGAATTTTGAGAAATAGGTTTAAAATGTCCATAATATTATTTTTTTAATCTTTTTTAAATTAATATTTTTGTATTATTTTTTTAATATTGAATGGTTTTTTAAATTAAAAAATTTAAAAGTATTAATTTAAAAAATTGAAAAATAATATTAGGGACCTTTTGAACACATTTTTCAAAACTTAGGGACTAAAAAAGTTTATTTTGAAAACTTAGGGACCTTATAAACATATTTCTTAAAATTCAGGGACTAAAAAGGTATGTTTTGAAAACTTAGGGACCAAACGCACATATTCGTCAAAACTCAGGGACCAAAAAGGTATTTGTCCCTTCTTTTTTTCTAACTTGTGTTCTAAGATAATTAGTTGGTTTTTATTAACATGGTAGGGCGATCCAATCGATTGGGTGAGTACACATCGAGATCATCATCAGTTTGTTGAGACGGAAAGAGACCCTCATAGTCCAATCCATGGATTTTGGTTTAGTCACCTAGGTTGGACCATGGACTCCTATGCTTTAACCAAACGAGTTTGTCCAAAATATGCCGAAGATTTTCAAGCGATAGATACAAACATTTTGGGATTTTGTTTGAAGTACGGAAGACCAGACAATGTCAGTGACTTAGAAGGGCAAATCTTCTATAGATTTATTCAAAAGACGTATTTGCTTCATCCATTGGCTCTTGGAATTGTGTTATATCTGATGGGAGGATTTCCATTTCTAGTTTGGTCAGGGGTAACAAATCTTCCACTTTTTCTTTCTATCCATTTTCTTTCCATTCCAAGTTTAAATTGTTATGACAGAAACCGTAAACCAACGAACAAGAATGAAAGGACGAAGAAAAACGACACACGGATTTACCTGGTTCACTAACGGTGTCTTAGCTACGTCCACGAGCAGAAGGAGAGAACAATCTTATTAATTATGAGGAGTATTCAGATTACAGAGATTATGGCAAAATAGGTTAAGATGGTTTATATATGACATCACTCTTTAACCCTAACATAATAGGCCCAAAATAAAAGTCCAAAACGAAAAATCATAATCTAATCTCTATACATAATTTAGTTTAGGGGGCGTTGCCCTCGAACCCCCATTGGGCGCTTCGCCCCTGGACCCCTACTCGCTGACCAGTCAGCGAGACCCCCGTACTTGGTCATTCAGAGCGCTTCATATTAGATTCAAGGCATATCAACGTAAATACTTTCTTCATTTATCAAAGTGGATATTTTCATTTGCAGGGGGTCGCAGTGACATGTGGTTACCATATAACTAGTATGGTAAATTCAATATGCCATAGTTGGGGCAAGCAACCGTGGAACACTCGTGATTCATCTAAGAATAATTGGTTCGACAATTCAAAACTTCTATATATTCTCCTATCAATCAGCTCTTGTGTGTTTGGATCAAAGACATTTTGTATGATATATTTACCTTGATTTAAACAATAATAATGTGTTGTTCTTATAGGTTGTTGGCTTTGCTTACATTTGGGGAGGGTTGGCACAATAACCACCATGCTTTTGAGTATTCAGCCAGACATGGGTTTGAATGGTGGCAACTTGATATCACTTGGTACATTATATTGTGCCTCCAAGCCATTGGATTGGCCACTGATGTCAAACTTCCTTCTGAAAAGCAAAAGCTTGCAAAAGCCCTCTCAACTCCCAAGAATACTTGA

mRNA sequence

ATGGAAGCACCAAGAGAAGTAGAAAAGAAGGTGGCAGGGTCTCGCAGGGCCCTTTGGAGGAGAAAATGGACATTTTTGGATATACGTTCTGCTACCTTCATCTTATTAGCACATTTACTTTGTATTTTTGCACCCTTTCATTTTAGTTGGGCTGCCCTTTTGGTTGCCTTTACACTTTACGTGGTCGTTTCGGTTTTCGGGATGGCACTCTCGTATCATAGAAATCTCACGCATAAGAGTTTCAAACTTCCTAAATGGCTGGAGTACCTGTTTGCGTATGTGGGAGTAATGGCACTTCAGGGCGATCCAATCGATTGGGTGAGTACACATCGAGATCATCATCAGTTTGTTGAGACGGAAAGAGACCCTCATAGTCCAATCCATGGATTTTGGTTTAGTCACCTAGGTTGGACCATGGACTCCTATGCTTTAACCAAACGAGTTTGTCCAAAATATGCCGAAGATTTTCAAGCGATAGATACAAACATTTTGGGATTTTGTTTGAAGTACGGAAGACCAGACAATGTCAGTGACTTAGAAGGGCAAATCTTCTATAGATTTATTCAAAAGACGTATTTGCTTCATCCATTGGCTCTTGGAATTGTGTTATATCTGATGGGAGGATTTCCATTTCTAGTTTGGTCAGGGAATAATTGGTTGTTGGCTTTGCTTACATTTGGGGAGGGTTGGCACAATAACCACCATGCTTTTGAGTATTCAGCCAGACATGGGTTTGAATGGTGGCAACTTGATATCACTTGGTACATTATATTGTGCCTCCAAGCCATTGGATTGGCCACTGATGTCAAACTTCCTTCTGAAAAGCAAAAGCTTGCAAAAGCCCTCTCAACTCCCAAGAATACTTGA

Coding sequence (CDS)

ATGGAAGCACCAAGAGAAGTAGAAAAGAAGGTGGCAGGGTCTCGCAGGGCCCTTTGGAGGAGAAAATGGACATTTTTGGATATACGTTCTGCTACCTTCATCTTATTAGCACATTTACTTTGTATTTTTGCACCCTTTCATTTTAGTTGGGCTGCCCTTTTGGTTGCCTTTACACTTTACGTGGTCGTTTCGGTTTTCGGGATGGCACTCTCGTATCATAGAAATCTCACGCATAAGAGTTTCAAACTTCCTAAATGGCTGGAGTACCTGTTTGCGTATGTGGGAGTAATGGCACTTCAGGGCGATCCAATCGATTGGGTGAGTACACATCGAGATCATCATCAGTTTGTTGAGACGGAAAGAGACCCTCATAGTCCAATCCATGGATTTTGGTTTAGTCACCTAGGTTGGACCATGGACTCCTATGCTTTAACCAAACGAGTTTGTCCAAAATATGCCGAAGATTTTCAAGCGATAGATACAAACATTTTGGGATTTTGTTTGAAGTACGGAAGACCAGACAATGTCAGTGACTTAGAAGGGCAAATCTTCTATAGATTTATTCAAAAGACGTATTTGCTTCATCCATTGGCTCTTGGAATTGTGTTATATCTGATGGGAGGATTTCCATTTCTAGTTTGGTCAGGGAATAATTGGTTGTTGGCTTTGCTTACATTTGGGGAGGGTTGGCACAATAACCACCATGCTTTTGAGTATTCAGCCAGACATGGGTTTGAATGGTGGCAACTTGATATCACTTGGTACATTATATTGTGCCTCCAAGCCATTGGATTGGCCACTGATGTCAAACTTCCTTCTGAAAAGCAAAAGCTTGCAAAAGCCCTCTCAACTCCCAAGAATACTTGA

Protein sequence

MEAPREVEKKVAGSRRALWRRKWTFLDIRSATFILLAHLLCIFAPFHFSWAALLVAFTLYVVVSVFGMALSYHRNLTHKSFKLPKWLEYLFAYVGVMALQGDPIDWVSTHRDHHQFVETERDPHSPIHGFWFSHLGWTMDSYALTKRVCPKYAEDFQAIDTNILGFCLKYGRPDNVSDLEGQIFYRFIQKTYLLHPLALGIVLYLMGGFPFLVWSGNNWLLALLTFGEGWHNNHHAFEYSARHGFEWWQLDITWYIILCLQAIGLATDVKLPSEKQKLAKALSTPKNT
Homology
[Close]
BLAST of Spg015789 vs. NCBI nr
Match: XP_008449174.1 (PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucumis melo])

HSP 1 Score: 426.4 bits (1095), Expect = 2.0e-115
Identity = 212/321 (66.04%), Postives = 236/321 (73.52%), Query Frame = 0 

Query: 1   MEAPREVEKKVAGSRRALWRRKWTFLDIRSATFILLAHLLCIFAPFHFSWAALLVAFTLY 60
           MEA  +  +K     RA WRRKWTF DIR+A +IL+ H LCIFAPFHF+W A  VAFTLY
Sbjct: 1   MEATMKDREK---PHRAFWRRKWTFSDIRTAIYILMTHSLCIFAPFHFNWKAFWVAFTLY 60

Query: 61  VVVSVFGMALSYHRNLTHKSFKLPKWLEYLFAYVGVMALQGDPIDWVSTHRDHHQFVETE 120
           V+V++FG+ LSYHRNLTHKSFKLPKWLEY FAY+GVMALQGDPIDWVSTHR HHQFVETE
Sbjct: 61  VLVAIFGITLSYHRNLTHKSFKLPKWLEYSFAYMGVMALQGDPIDWVSTHRHHHQFVETE 120

Query: 121 RDPHSPIHGFWFSHLGWTMDSYALTKRVCPKYAEDFQAIDTNILGFCLKYGRPDNVSDLE 180
           RDPHSPIHG W+SH+GWTM+S ALTKR                      +GRP+NVSDLE
Sbjct: 121 RDPHSPIHGLWYSHMGWTMNSRALTKR----------------------HGRPNNVSDLE 180

Query: 181 GQIFYRFIQKTYLLHPLALGIVLYLMGGFPFLVWSG------------------------ 240
            QIFYR IQKTYLLHPLALGIVL+L+GGFPFLVWSG                        
Sbjct: 181 DQIFYRIIQKTYLLHPLALGIVLFLIGGFPFLVWSGGVAVTWGYHVTSMVNSICHSWGSQ 240

Query: 241 ---------NNWLLALLTFGEGWHNNHHAFEYSARHGFEWWQLDITWYIILCLQAIGLAT 289
                    NNWL++L+TFGEGWHNNHHAFEYSARHG EWWQLDI WYIILCLQA+GLAT
Sbjct: 241 PWKTRDSSKNNWLVSLVTFGEGWHNNHHAFEYSARHGLEWWQLDIPWYIILCLQALGLAT 296

[Close]
BLAST of Spg015789 vs. NCBI nr
Match: XP_022153644.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 384.8 bits (987), Expect = 6.5e-103
Identity = 183/307 (59.61%), Postives = 216/307 (70.36%), Query Frame = 0 

Query: 1   MEAPREVEKKVAGSRRALWRRKWTFLDIRSATFILLAHLLCIFAPFHFSWAALLVAFTLY 60
           MEA ++  K++    R   RRKWT LD   A  + L HLLCI APF F+W+A  VAF LY
Sbjct: 1   MEASKQEPKEMMKPPRPYERRKWTKLDKDIACSLFLVHLLCILAPFQFNWSAFWVAFVLY 60

Query: 61  VVVSVFGMALSYHRNLTHKSFKLPKWLEYLFAYVGVMALQGDPIDWVSTHRDHHQFVETE 120
           +V  +FGM LSYHRNL HKSFKLPKWLEYLFAY GV ALQGDP+DWVSTHR HHQFV+TE
Sbjct: 61  IVTGLFGMTLSYHRNLAHKSFKLPKWLEYLFAYCGVQALQGDPMDWVSTHRCHHQFVDTE 120

Query: 121 RDPHSPIHGFWFSHLGWTMDSYALTKRVCPKYAEDFQAIDTNILGFCLKYGRPDNVSDLE 180
           RDPHSPI GFWFSH+ W  DSY LTK+VCPKY  +FQ ++ NI     K+G+P+NV+DLE
Sbjct: 121 RDPHSPIRGFWFSHILWLFDSYNLTKKVCPKYFSNFQKLERNIFVLFSKHGKPENVADLE 180

Query: 181 GQIFYRFIQKTYLLHPLALGIVLYLMGGFPFLVW-------------------------- 240
            Q FYRFI +TYLLHP+ L ++LY++GG PFLVW                          
Sbjct: 181 KQAFYRFIHQTYLLHPIVLAVILYVVGGIPFLVWGMAVRIVVVLHFTLMVNSVCHIWGKQ 240

Query: 241 -------SGNNWLLALLTFGEGWHNNHHAFEYSARHGFEWWQLDITWYIILCLQAIGLAT 275
                  S NNW ++L+TFGEGWHNNHHAFEYSA+ G EWWQLD  WY+IL LQAIG+AT
Sbjct: 241 SWTTGDMSKNNWWVSLITFGEGWHNNHHAFEYSAKFGLEWWQLDFGWYMILFLQAIGVAT 300

[Close]
BLAST of Spg015789 vs. NCBI nr
Match: XP_022153481.1 (acyl-CoA C20 Delta5-desaturase-like isoform X1 [Momordica charantia])

HSP 1 Score: 384.4 bits (986), Expect = 8.5e-103
Identity = 183/315 (58.10%), Postives = 218/315 (69.21%), Query Frame = 0 

Query: 6   EVEKKVAGSRRALWRRKWTFLDIRSATFILLAHLLCIFAPFHFSWAALLVAFTLYVVVSV 65
           E E  V   RR ++RRKWT LD RSA  +L+ HLLC+ APF F+W A  +AF LY++  +
Sbjct: 27  EDEAAVRDRRRPIFRRKWTSLDKRSAGALLVMHLLCLLAPFQFTWPAFWLAFVLYIITGL 86

Query: 66  FGMALSYHRNLTHKSFKLPKWLEYLFAYVGVMALQGDPIDWVSTHRDHHQFVETERDPHS 125
           FG+ LSYHR+LTHKSFKLPKWLEY FAY+GV   QGDPIDWVSTHR HHQFV+TERDPH+
Sbjct: 87  FGLTLSYHRHLTHKSFKLPKWLEYTFAYIGVHTFQGDPIDWVSTHRYHHQFVDTERDPHT 146

Query: 126 PIHGFWFSHLGWTMDSYALTKRVCPKYAEDFQAID------TNILGFCLKYGRPDNVSDL 185
           P  GFWF H+ W  DSY LTK+VCPKY  DF+  D       N+    LKYGRP+N  DL
Sbjct: 147 PTQGFWFGHIAWIWDSYGLTKKVCPKYVRDFEVRDGKRSRLRNMFVGYLKYGRPNNAEDL 206

Query: 186 EGQIFYRFIQKTYLLHPLALGIVLYLMGGFPFLVW------------------------- 245
           + Q FYRF+++TY+LH + L +VLY +GG PFLVW                         
Sbjct: 207 QKQAFYRFLRRTYILHHVGLAVVLYAVGGLPFLVWGMGVRTVCFQHVTFSVNSLGHMWGN 266

Query: 246 --------SGNNWLLALLTFGEGWHNNHHAFEYSARHGFEWWQLDITWYIILCLQAIGLA 280
                   S NNW+LA+L FGEGWHNNHHAFEYSARHG EWWQLD+TW++IL LQA+GLA
Sbjct: 267 QQWNTGDQSRNNWVLAMLIFGEGWHNNHHAFEYSARHGLEWWQLDVTWWVILLLQALGLA 326

[Close]
BLAST of Spg015789 vs. NCBI nr
Match: XP_022153442.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 383.6 bits (984), Expect = 1.5e-102
Identity = 183/306 (59.80%), Postives = 215/306 (70.26%), Query Frame = 0 

Query: 15  RRALWRRKWTFLDIRSATFILLAHLLCIFAPFHFSWAALLVAFTLYVVVSVFGMALSYHR 74
           RR   +RKWT LD RSA  IL  HLLC+ APFH +WAA  VAF LY +  +FG+ LSYHR
Sbjct: 28  RRGFLKRKWTILDRRSAGGILFLHLLCLLAPFHCNWAAFWVAFALYFITGLFGITLSYHR 87

Query: 75  NLTHKSFKLPKWLEYLFAYVGVMALQGDPIDWVSTHRDHHQFVETERDPHSPIHGFWFSH 134
           NL HKSFKLPKWLEYLFAY+G+ A QGDPIDWVSTHR HHQFV+TERDPHSP+ GFWFSH
Sbjct: 88  NLAHKSFKLPKWLEYLFAYLGLHAFQGDPIDWVSTHRYHHQFVDTERDPHSPLEGFWFSH 147

Query: 135 LGWTMDSYALTKRVCPKYAEDFQAID------TNILGFCLKYGRPDNVSDLEGQIFYRFI 194
           + W  D+Y LTK+VCPK  EDF+  D      +NI    +KYGRP+NV DL+ Q FYRF+
Sbjct: 148 ITWMFDAYTLTKKVCPKNVEDFEEKDAKRKRFSNIFVAQVKYGRPNNVDDLQKQAFYRFL 207

Query: 195 QKTYLLHPLALGIVLYLMGGFPFLVW---------------------------------S 254
           ++TY+LHP AL +VLY MGG PF+VW                                 S
Sbjct: 208 RRTYILHPTALALVLYAMGGLPFVVWGMGVRLVWFLHITFFINSACHIWGYRRWNTTDLS 267

Query: 255 GNNWLLALLTFGEGWHNNHHAFEYSARHGFEWWQLDITWYIILCLQAIGLATDVKLPS-- 280
            NNW+  LL FGEGWHNNHHAFEYSARHG +WW+ D+TW+++L LQ IGLATDVKLPS  
Sbjct: 268 KNNWVFGLLAFGEGWHNNHHAFEYSARHGLQWWEFDLTWWVVLFLQTIGLATDVKLPSQG 327

[Close]
BLAST of Spg015789 vs. NCBI nr
Match: XP_022956260.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 383.6 bits (984), Expect = 1.5e-102
Identity = 184/319 (57.68%), Postives = 217/319 (68.03%), Query Frame = 0 

Query: 2   EAPREVEKKVAGSRRALWRRKWTFLDIRSATFILLAHLLCIFAPFHFSWAALLVAFTLYV 61
           +A ++  K++    R+ WRRKWT LD R A  ILL HLLCI APFHF+W A  VA TL +
Sbjct: 19  DASKQEPKEMVKPPRSFWRRKWTKLDKRVAHVILLLHLLCILAPFHFTWPAFRVAVTLQI 78

Query: 62  VVSVFGMALSYHRNLTHKSFKLPKWLEYLFAYVGVMALQGDPIDWVSTHRDHHQFVETER 121
           +  +FGM +SYHRNL HKSF++PKWLEYLFAY GV ALQGDPIDWVSTHR HHQFV+TER
Sbjct: 79  ITGLFGMTISYHRNLAHKSFQIPKWLEYLFAYCGVHALQGDPIDWVSTHRCHHQFVDTER 138

Query: 122 DPHSPIHGFWFSHLGWTMDSYALTKRVCPKYAEDFQAIDTNILGFCLKYGRPDNVSDLEG 181
           DPHSPI GFW SH+ W  DSY LT +V PKY+ D Q I+ N+  +  KY +P+NV DLE 
Sbjct: 139 DPHSPIQGFWMSHIMWLFDSYTLTDKVSPKYSSDLQKIEKNMFVYFSKYRKPENVGDLER 198

Query: 182 QIFYRFIQKTYLLHPLALGIVLYLMGGFPFLVW--------------------------- 241
           Q FYRFI KTYLLHP+AL  +LY +GGFPFL+W                           
Sbjct: 199 QTFYRFIHKTYLLHPMALAAILYTVGGFPFLIWGMCVRIVVIFHSTFMVNSVCHMWGQQH 258

Query: 242 ------SGNNWLLALLTFGEGWHNNHHAFEYSARHGFEWWQLDITWYIILCLQAIGLATD 288
                 S NNW + L +FGEGWHNNHHAF+YSAR G EWWQLD  WY+I  LQ IGLATD
Sbjct: 259 WRTGDLSRNNWWVGLTSFGEGWHNNHHAFKYSARLGLEWWQLDAGWYVIRILQVIGLATD 318

[Close]
BLAST of Spg015789 vs. ExPASy Swiss-Prot
Match: Q949X0 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3 PE=1 SV=2)

HSP 1 Score: 329.7 bits (844), Expect = 3.3e-89
Identity = 157/299 (52.51%), Postives = 192/299 (64.21%), Query Frame = 0 

Query: 19  WRRKWTFLDIRSATFILLAHLLCIFAPFHFSWAALLVAFTLYVVVSVFGMALSYHRNLTH 78
           W R+W  +D  +   +L  HLL + APF F+W A+ VAF LY+V  + G+ LS+HRNL+H
Sbjct: 94  WEREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSFHRNLSH 153

Query: 79  KSFKLPKWLEYLFAYVGVMALQGDPIDWVSTHRDHHQFVETERDPHSPIHGFWFSHLGWT 138
           K+FKLPKWLEYLFAY G  ALQG+PIDWVSTHR HHQF +++RDPHSP+ GFWFSH+ W 
Sbjct: 154 KAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWFSHMNWM 213

Query: 139 MDSYALTKRVCPKYAEDFQAIDTNILGFCLKYGRPDNVSDLEGQIFYRFIQKTYLLHPLA 198
            D+  +T+R                       G P+NV DLE Q FYRF++ TY+LHPLA
Sbjct: 214 FDTNTITQRC----------------------GEPNNVGDLEKQPFYRFLRTTYILHPLA 273

Query: 199 LGIVLYLMGGFPFLVW---------------------------------SGNNWLLALLT 258
           L + LY MGGFPF+VW                                 S NNW +A L 
Sbjct: 274 LAVALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSKNNWWVAALA 333

Query: 259 FGEGWHNNHHAFEYSARHGFEWWQLDITWYIILCLQAIGLATDVKLPSEKQKLAKALST 285
           FGEGWHNNHHAFE+SARHG EWWQLD+TWY++  LQAIGLATDVKLPSE QK   A ++
Sbjct: 334 FGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLPSEAQKQRMAFTS 370

[Close]
BLAST of Spg015789 vs. ExPASy Swiss-Prot
Match: P0DOW2 (Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 2.9e-77
Identity = 144/297 (48.48%), Postives = 178/297 (59.93%), Query Frame = 0 

Query: 14  SRRALWRRKWTFLDIRSATFILLAHLLCIFAPFHFSWAALLVAFTLYVVVSVFGMALSYH 73
           +++  W RKW   D+    FI   H+LC+FAP  FSW +  V F LY +  VFG  LS+H
Sbjct: 31  TKKITWVRKWNSTDVFHILFIGGLHVLCLFAPSTFSWKSFWVCFALYAICGVFGTTLSFH 90

Query: 74  RNLTHKSFKLPKWLEYLFAYVGVMALQGDPIDWVSTHRDHHQFVETERDPHSPIHGFWFS 133
           RNLTH+SFKLPK+LEY FAYVG+ ALQGDP+ WVSTHR HH++ +T  DPHSPI GFWF 
Sbjct: 91  RNLTHRSFKLPKYLEYFFAYVGLHALQGDPVWWVSTHRYHHKYTDTYLDPHSPIEGFWFC 150

Query: 134 HLGWTMDSYALTKRVCPKYAEDFQAIDTNILGFCLKYGRPDNVSDLEGQIFYRFIQKTYL 193
           H+ W  DS         KY  +             K GR +N  DL  Q +YRF+++T++
Sbjct: 151 HIFWLFDS---------KYIIE-------------KCGRYENAGDLMKQSYYRFLERTFV 210

Query: 194 LHPLALGIVLYLMGGFPFLVW---------------------------------SGNNWL 253
            H      +LYL GGFPF+VW                                 S NNW 
Sbjct: 211 YHVYLQAALLYLFGGFPFIVWGMAVRTILGFHLSWLVNSVCHRWGNRPWNTGDLSTNNWF 270

Query: 254 LALLTFGEGWHNNHHAFEYSARHGFEWWQLDITWYIILCLQAIGLATDVKLPSEKQK 278
           +A+LT GEGWHNNHHAFEYSARHG EWWQ+D TWYII  L+ +GLATD+K+PSE  K
Sbjct: 271 IAMLTSGEGWHNNHHAFEYSARHGIEWWQIDTTWYIIKLLEYLGLATDIKVPSEIHK 305

[Close]
BLAST of Spg015789 vs. ExPASy Swiss-Prot
Match: P0DOW3 (Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 2.9e-77
Identity = 147/297 (49.49%), Postives = 180/297 (60.61%), Query Frame = 0 

Query: 14  SRRALWRRKWTFLDIRSATFILLAHLLCIFAPFHFSWAALLVAFTLYVVVSVFGMALSYH 73
           +++  W R W   DI     +   H+LC+ APF FSW+A  ++ TLY V  VFG  LSYH
Sbjct: 28  TKKITWVRVWNSTDIFHIILVGGLHVLCLSAPFTFSWSAFWLSLTLYAVCGVFGTTLSYH 87

Query: 74  RNLTHKSFKLPKWLEYLFAYVGVMALQGDPIDWVSTHRDHHQFVETERDPHSPIHGFWFS 133
           RNLTH+SFKLPK+LEY FAYVG+ ALQGDP+ WVSTHR HH+F +T  DPHSPI GFWFS
Sbjct: 88  RNLTHRSFKLPKYLEYFFAYVGLHALQGDPVWWVSTHRYHHKFTDTYLDPHSPIEGFWFS 147

Query: 134 HLGWTMDSYALTKRVCPKYAEDFQAIDTNILGFCLKYGRPDNVSDLEGQIFYRFIQKTYL 193
           H+ W  DS         KY          IL  C   GR +N  DL  Q +YRF+++T++
Sbjct: 148 HIFWLFDS---------KY----------ILEEC---GRYENAGDLLKQSYYRFLERTFV 207

Query: 194 LHPLALGIVLYLMGGFPFLVW---------------------------------SGNNWL 253
            H      +LY+ GGFPF+VW                                 S NNW 
Sbjct: 208 FHVYLQAALLYMFGGFPFIVWGMAVRTVWGFHASWLVNSVCHRYGHQAWDTGDLSTNNWF 267

Query: 254 LALLTFGEGWHNNHHAFEYSARHGFEWWQLDITWYIILCLQAIGLATDVKLPSEKQK 278
           +A+LT GEGWHNNHHAFEYSARHG EWWQ+D TWY+I  L+ +GLATDVK+PSE  K
Sbjct: 268 IAMLTSGEGWHNNHHAFEYSARHGIEWWQIDTTWYVIKLLEYLGLATDVKVPSEVHK 302

[Close]
BLAST of Spg015789 vs. ExPASy Swiss-Prot
Match: O65797 (Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 8.3e-77
Identity = 146/301 (48.50%), Postives = 180/301 (59.80%), Query Frame = 0 

Query: 15  RRALWRRKWTFLDIRSATFILLAHLLCIFAPFHFSWAALLVAFTLYVVVSVFGMALSYHR 74
           +RA+W RKW  LDI  A   L  H LC+ APF+F+W AL VA  +Y V  + G+ +SYHR
Sbjct: 26  KRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRVALIVYTVGGL-GITVSYHR 85

Query: 75  NLTHKSFKLPKWLEYLFAYVGVMALQGDPIDWVSTHRDHHQFVETERDPHSPIHGFWFSH 134
           NL H+SFK+PKWLEY FAY G++A+QGDPIDWVSTHR HHQF +++RDPHSP  GFWFSH
Sbjct: 86  NLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDSDRDPHSPNEGFWFSH 145

Query: 135 LGWTMDSYALTKRVCPKYAEDFQAIDTNILGFCLKYGRPDNVSDLEGQIFYRFIQKTYLL 194
           L W  D+  L +                      K GR  NV DL+ Q +Y+F+Q+T L 
Sbjct: 146 LLWLFDTGYLVE----------------------KCGRRTNVEDLKRQWYYKFLQRTVLY 205

Query: 195 HPLALGIVLYLMGGFPFLVW---------------------------------SGNNWLL 254
           H L  G +LY  GG  FL W                                 S N W L
Sbjct: 206 HILTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCLINSLCHVWGSRTWKTNDTSRNVWWL 265

Query: 255 ALLTFGEGWHNNHHAFEYSARHGFEWWQLDITWYIILCLQAIGLATDVKLPSEKQKLAKA 283
           ++ +FGE WHNNHHAFE SAR G EWWQ+DI+WYI+  L+ IGLATDVKLPSE Q+   A
Sbjct: 266 SVFSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFLEIIGLATDVKLPSESQRRRMA 303

[Close]
BLAST of Spg015789 vs. ExPASy Swiss-Prot
Match: Q9FV68 (Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii OX=28973 PE=1 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 1.9e-76
Identity = 147/311 (47.27%), Postives = 185/311 (59.49%), Query Frame = 0 

Query: 1   MEAPREVEKKVAGSRRALWRRKWTFLDIRSATFILLAHLLCIFAPFHFSWAALLVAFTLY 60
           +E  R  E  +    +  WRRKW   DI++A  +L+ H L   APF+FSW A  ++F L 
Sbjct: 59  LERFRTSEVVLERKAKGFWRRKWNPRDIQNAVTLLVLHALAAMAPFYFSWDAFWISFILL 118

Query: 61  VVVS-VFGMALSYHRNLTHKSFKLPKWLEYLFAYVGVMALQGDPIDWVSTHRDHHQFVET 120
              S V G+ L +HR LTH  FKLPK +EY FAY G +ALQGDP++WVS HR HHQFV+T
Sbjct: 119 GFASGVLGITLCFHRCLTHGGFKLPKLVEYFFAYCGSLALQGDPMEWVSNHRYHHQFVDT 178

Query: 121 ERDPHSPIHGFWFSHLGWTMDSYALTKRVCPKYAEDFQAIDTNILGFCLKYGRPDNVSDL 180
           ERD HSP  GFWF H+GW +D     ++                     + GR +NV+DL
Sbjct: 179 ERDVHSPTQGFWFCHIGWVLDKDLFVEK---------------------RGGRRNNVNDL 238

Query: 181 EGQIFYRFIQKTYLLHPLALGIVLYLMGGFPFLVW------------------------- 240
           + Q FYRF+QKTY+ H LAL  +LY +GGFP++VW                         
Sbjct: 239 KKQAFYRFLQKTYMYHQLALIALLYYVGGFPYIVWGMGFRLVFMFHSTFAINSVCHKWGG 298

Query: 241 --------SGNNWLLALLTFGEGWHNNHHAFEYSARHGFEWWQLDITWYIILCLQAIGLA 278
                   S NN  +AL  FGEGWHNNHHAFE SARHG EWW++D+TWY+I  LQAIGLA
Sbjct: 299 RPWNTGDLSTNNMFVALCAFGEGWHNNHHAFEQSARHGLEWWEIDVTWYVIRTLQAIGLA 348

[Close]
BLAST of Spg015789 vs. ExPASy TrEMBL
Match: A0A1S3BMC9 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103491124 PE=3 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 9.5e-116
Identity = 212/321 (66.04%), Postives = 236/321 (73.52%), Query Frame = 0 

Query: 1   MEAPREVEKKVAGSRRALWRRKWTFLDIRSATFILLAHLLCIFAPFHFSWAALLVAFTLY 60
           MEA  +  +K     RA WRRKWTF DIR+A +IL+ H LCIFAPFHF+W A  VAFTLY
Sbjct: 1   MEATMKDREK---PHRAFWRRKWTFSDIRTAIYILMTHSLCIFAPFHFNWKAFWVAFTLY 60

Query: 61  VVVSVFGMALSYHRNLTHKSFKLPKWLEYLFAYVGVMALQGDPIDWVSTHRDHHQFVETE 120
           V+V++FG+ LSYHRNLTHKSFKLPKWLEY FAY+GVMALQGDPIDWVSTHR HHQFVETE
Sbjct: 61  VLVAIFGITLSYHRNLTHKSFKLPKWLEYSFAYMGVMALQGDPIDWVSTHRHHHQFVETE 120

Query: 121 RDPHSPIHGFWFSHLGWTMDSYALTKRVCPKYAEDFQAIDTNILGFCLKYGRPDNVSDLE 180
           RDPHSPIHG W+SH+GWTM+S ALTKR                      +GRP+NVSDLE
Sbjct: 121 RDPHSPIHGLWYSHMGWTMNSRALTKR----------------------HGRPNNVSDLE 180

Query: 181 GQIFYRFIQKTYLLHPLALGIVLYLMGGFPFLVWSG------------------------ 240
            QIFYR IQKTYLLHPLALGIVL+L+GGFPFLVWSG                        
Sbjct: 181 DQIFYRIIQKTYLLHPLALGIVLFLIGGFPFLVWSGGVAVTWGYHVTSMVNSICHSWGSQ 240

Query: 241 ---------NNWLLALLTFGEGWHNNHHAFEYSARHGFEWWQLDITWYIILCLQAIGLAT 289
                    NNWL++L+TFGEGWHNNHHAFEYSARHG EWWQLDI WYIILCLQA+GLAT
Sbjct: 241 PWKTRDSSKNNWLVSLVTFGEGWHNNHHAFEYSARHGLEWWQLDIPWYIILCLQALGLAT 296

[Close]
BLAST of Spg015789 vs. ExPASy TrEMBL
Match: A0A6J1DJP0 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111021101 PE=3 SV=1)

HSP 1 Score: 384.8 bits (987), Expect = 3.2e-103
Identity = 183/307 (59.61%), Postives = 216/307 (70.36%), Query Frame = 0 

Query: 1   MEAPREVEKKVAGSRRALWRRKWTFLDIRSATFILLAHLLCIFAPFHFSWAALLVAFTLY 60
           MEA ++  K++    R   RRKWT LD   A  + L HLLCI APF F+W+A  VAF LY
Sbjct: 1   MEASKQEPKEMMKPPRPYERRKWTKLDKDIACSLFLVHLLCILAPFQFNWSAFWVAFVLY 60

Query: 61  VVVSVFGMALSYHRNLTHKSFKLPKWLEYLFAYVGVMALQGDPIDWVSTHRDHHQFVETE 120
           +V  +FGM LSYHRNL HKSFKLPKWLEYLFAY GV ALQGDP+DWVSTHR HHQFV+TE
Sbjct: 61  IVTGLFGMTLSYHRNLAHKSFKLPKWLEYLFAYCGVQALQGDPMDWVSTHRCHHQFVDTE 120

Query: 121 RDPHSPIHGFWFSHLGWTMDSYALTKRVCPKYAEDFQAIDTNILGFCLKYGRPDNVSDLE 180
           RDPHSPI GFWFSH+ W  DSY LTK+VCPKY  +FQ ++ NI     K+G+P+NV+DLE
Sbjct: 121 RDPHSPIRGFWFSHILWLFDSYNLTKKVCPKYFSNFQKLERNIFVLFSKHGKPENVADLE 180

Query: 181 GQIFYRFIQKTYLLHPLALGIVLYLMGGFPFLVW-------------------------- 240
            Q FYRFI +TYLLHP+ L ++LY++GG PFLVW                          
Sbjct: 181 KQAFYRFIHQTYLLHPIVLAVILYVVGGIPFLVWGMAVRIVVVLHFTLMVNSVCHIWGKQ 240

Query: 241 -------SGNNWLLALLTFGEGWHNNHHAFEYSARHGFEWWQLDITWYIILCLQAIGLAT 275
                  S NNW ++L+TFGEGWHNNHHAFEYSA+ G EWWQLD  WY+IL LQAIG+AT
Sbjct: 241 SWTTGDMSKNNWWVSLITFGEGWHNNHHAFEYSAKFGLEWWQLDFGWYMILFLQAIGVAT 300

[Close]
BLAST of Spg015789 vs. ExPASy TrEMBL
Match: A0A6J1DKS5 (acyl-CoA C20 Delta5-desaturase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111020979 PE=3 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 4.1e-103
Identity = 183/315 (58.10%), Postives = 218/315 (69.21%), Query Frame = 0 

Query: 6   EVEKKVAGSRRALWRRKWTFLDIRSATFILLAHLLCIFAPFHFSWAALLVAFTLYVVVSV 65
           E E  V   RR ++RRKWT LD RSA  +L+ HLLC+ APF F+W A  +AF LY++  +
Sbjct: 27  EDEAAVRDRRRPIFRRKWTSLDKRSAGALLVMHLLCLLAPFQFTWPAFWLAFVLYIITGL 86

Query: 66  FGMALSYHRNLTHKSFKLPKWLEYLFAYVGVMALQGDPIDWVSTHRDHHQFVETERDPHS 125
           FG+ LSYHR+LTHKSFKLPKWLEY FAY+GV   QGDPIDWVSTHR HHQFV+TERDPH+
Sbjct: 87  FGLTLSYHRHLTHKSFKLPKWLEYTFAYIGVHTFQGDPIDWVSTHRYHHQFVDTERDPHT 146

Query: 126 PIHGFWFSHLGWTMDSYALTKRVCPKYAEDFQAID------TNILGFCLKYGRPDNVSDL 185
           P  GFWF H+ W  DSY LTK+VCPKY  DF+  D       N+    LKYGRP+N  DL
Sbjct: 147 PTQGFWFGHIAWIWDSYGLTKKVCPKYVRDFEVRDGKRSRLRNMFVGYLKYGRPNNAEDL 206

Query: 186 EGQIFYRFIQKTYLLHPLALGIVLYLMGGFPFLVW------------------------- 245
           + Q FYRF+++TY+LH + L +VLY +GG PFLVW                         
Sbjct: 207 QKQAFYRFLRRTYILHHVGLAVVLYAVGGLPFLVWGMGVRTVCFQHVTFSVNSLGHMWGN 266

Query: 246 --------SGNNWLLALLTFGEGWHNNHHAFEYSARHGFEWWQLDITWYIILCLQAIGLA 280
                   S NNW+LA+L FGEGWHNNHHAFEYSARHG EWWQLD+TW++IL LQA+GLA
Sbjct: 267 QQWNTGDQSRNNWVLAMLIFGEGWHNNHHAFEYSARHGLEWWQLDVTWWVILLLQALGLA 326

[Close]
BLAST of Spg015789 vs. ExPASy TrEMBL
Match: A0A6J1DIX0 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111020949 PE=3 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 7.0e-103
Identity = 183/306 (59.80%), Postives = 215/306 (70.26%), Query Frame = 0 

Query: 15  RRALWRRKWTFLDIRSATFILLAHLLCIFAPFHFSWAALLVAFTLYVVVSVFGMALSYHR 74
           RR   +RKWT LD RSA  IL  HLLC+ APFH +WAA  VAF LY +  +FG+ LSYHR
Sbjct: 28  RRGFLKRKWTILDRRSAGGILFLHLLCLLAPFHCNWAAFWVAFALYFITGLFGITLSYHR 87

Query: 75  NLTHKSFKLPKWLEYLFAYVGVMALQGDPIDWVSTHRDHHQFVETERDPHSPIHGFWFSH 134
           NL HKSFKLPKWLEYLFAY+G+ A QGDPIDWVSTHR HHQFV+TERDPHSP+ GFWFSH
Sbjct: 88  NLAHKSFKLPKWLEYLFAYLGLHAFQGDPIDWVSTHRYHHQFVDTERDPHSPLEGFWFSH 147

Query: 135 LGWTMDSYALTKRVCPKYAEDFQAID------TNILGFCLKYGRPDNVSDLEGQIFYRFI 194
           + W  D+Y LTK+VCPK  EDF+  D      +NI    +KYGRP+NV DL+ Q FYRF+
Sbjct: 148 ITWMFDAYTLTKKVCPKNVEDFEEKDAKRKRFSNIFVAQVKYGRPNNVDDLQKQAFYRFL 207

Query: 195 QKTYLLHPLALGIVLYLMGGFPFLVW---------------------------------S 254
           ++TY+LHP AL +VLY MGG PF+VW                                 S
Sbjct: 208 RRTYILHPTALALVLYAMGGLPFVVWGMGVRLVWFLHITFFINSACHIWGYRRWNTTDLS 267

Query: 255 GNNWLLALLTFGEGWHNNHHAFEYSARHGFEWWQLDITWYIILCLQAIGLATDVKLPS-- 280
            NNW+  LL FGEGWHNNHHAFEYSARHG +WW+ D+TW+++L LQ IGLATDVKLPS  
Sbjct: 268 KNNWVFGLLAFGEGWHNNHHAFEYSARHGLQWWEFDLTWWVVLFLQTIGLATDVKLPSQG 327

[Close]
BLAST of Spg015789 vs. ExPASy TrEMBL
Match: A0A6J1GXC1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111458012 PE=3 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 7.0e-103
Identity = 184/319 (57.68%), Postives = 217/319 (68.03%), Query Frame = 0 

Query: 2   EAPREVEKKVAGSRRALWRRKWTFLDIRSATFILLAHLLCIFAPFHFSWAALLVAFTLYV 61
           +A ++  K++    R+ WRRKWT LD R A  ILL HLLCI APFHF+W A  VA TL +
Sbjct: 19  DASKQEPKEMVKPPRSFWRRKWTKLDKRVAHVILLLHLLCILAPFHFTWPAFRVAVTLQI 78

Query: 62  VVSVFGMALSYHRNLTHKSFKLPKWLEYLFAYVGVMALQGDPIDWVSTHRDHHQFVETER 121
           +  +FGM +SYHRNL HKSF++PKWLEYLFAY GV ALQGDPIDWVSTHR HHQFV+TER
Sbjct: 79  ITGLFGMTISYHRNLAHKSFQIPKWLEYLFAYCGVHALQGDPIDWVSTHRCHHQFVDTER 138

Query: 122 DPHSPIHGFWFSHLGWTMDSYALTKRVCPKYAEDFQAIDTNILGFCLKYGRPDNVSDLEG 181
           DPHSPI GFW SH+ W  DSY LT +V PKY+ D Q I+ N+  +  KY +P+NV DLE 
Sbjct: 139 DPHSPIQGFWMSHIMWLFDSYTLTDKVSPKYSSDLQKIEKNMFVYFSKYRKPENVGDLER 198

Query: 182 QIFYRFIQKTYLLHPLALGIVLYLMGGFPFLVW--------------------------- 241
           Q FYRFI KTYLLHP+AL  +LY +GGFPFL+W                           
Sbjct: 199 QTFYRFIHKTYLLHPMALAAILYTVGGFPFLIWGMCVRIVVIFHSTFMVNSVCHMWGQQH 258

Query: 242 ------SGNNWLLALLTFGEGWHNNHHAFEYSARHGFEWWQLDITWYIILCLQAIGLATD 288
                 S NNW + L +FGEGWHNNHHAF+YSAR G EWWQLD  WY+I  LQ IGLATD
Sbjct: 259 WRTGDLSRNNWWVGLTSFGEGWHNNHHAFKYSARLGLEWWQLDAGWYVIRILQVIGLATD 318

[Close]
BLAST of Spg015789 vs. TAIR 10
Match: AT3G15850.1 (fatty acid desaturase 5 )

HSP 1 Score: 329.7 bits (844), Expect = 2.3e-90
Identity = 157/299 (52.51%), Postives = 192/299 (64.21%), Query Frame = 0 

Query: 19  WRRKWTFLDIRSATFILLAHLLCIFAPFHFSWAALLVAFTLYVVVSVFGMALSYHRNLTH 78
           W R+W  +D  +   +L  HLL + APF F+W A+ VAF LY+V  + G+ LS+HRNL+H
Sbjct: 94  WEREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSFHRNLSH 153

Query: 79  KSFKLPKWLEYLFAYVGVMALQGDPIDWVSTHRDHHQFVETERDPHSPIHGFWFSHLGWT 138
           K+FKLPKWLEYLFAY G  ALQG+PIDWVSTHR HHQF +++RDPHSP+ GFWFSH+ W 
Sbjct: 154 KAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWFSHMNWM 213

Query: 139 MDSYALTKRVCPKYAEDFQAIDTNILGFCLKYGRPDNVSDLEGQIFYRFIQKTYLLHPLA 198
            D+  +T+R                       G P+NV DLE Q FYRF++ TY+LHPLA
Sbjct: 214 FDTNTITQRC----------------------GEPNNVGDLEKQPFYRFLRTTYILHPLA 273

Query: 199 LGIVLYLMGGFPFLVW---------------------------------SGNNWLLALLT 258
           L + LY MGGFPF+VW                                 S NNW +A L 
Sbjct: 274 LAVALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSKNNWWVAALA 333

Query: 259 FGEGWHNNHHAFEYSARHGFEWWQLDITWYIILCLQAIGLATDVKLPSEKQKLAKALST 285
           FGEGWHNNHHAFE+SARHG EWWQLD+TWY++  LQAIGLATDVKLPSE QK   A ++
Sbjct: 334 FGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLPSEAQKQRMAFTS 370

[Close]
BLAST of Spg015789 vs. TAIR 10
Match: AT1G06080.1 (delta 9 desaturase 1 )

HSP 1 Score: 288.5 bits (737), Expect = 5.9e-78
Identity = 146/301 (48.50%), Postives = 180/301 (59.80%), Query Frame = 0 

Query: 15  RRALWRRKWTFLDIRSATFILLAHLLCIFAPFHFSWAALLVAFTLYVVVSVFGMALSYHR 74
           +RA+W RKW  LDI  A   L  H LC+ APF+F+W AL VA  +Y V  + G+ +SYHR
Sbjct: 26  KRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRVALIVYTVGGL-GITVSYHR 85

Query: 75  NLTHKSFKLPKWLEYLFAYVGVMALQGDPIDWVSTHRDHHQFVETERDPHSPIHGFWFSH 134
           NL H+SFK+PKWLEY FAY G++A+QGDPIDWVSTHR HHQF +++RDPHSP  GFWFSH
Sbjct: 86  NLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDSDRDPHSPNEGFWFSH 145

Query: 135 LGWTMDSYALTKRVCPKYAEDFQAIDTNILGFCLKYGRPDNVSDLEGQIFYRFIQKTYLL 194
           L W  D+  L +                      K GR  NV DL+ Q +Y+F+Q+T L 
Sbjct: 146 LLWLFDTGYLVE----------------------KCGRRTNVEDLKRQWYYKFLQRTVLY 205

Query: 195 HPLALGIVLYLMGGFPFLVW---------------------------------SGNNWLL 254
           H L  G +LY  GG  FL W                                 S N W L
Sbjct: 206 HILTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCLINSLCHVWGSRTWKTNDTSRNVWWL 265

Query: 255 ALLTFGEGWHNNHHAFEYSARHGFEWWQLDITWYIILCLQAIGLATDVKLPSEKQKLAKA 283
           ++ +FGE WHNNHHAFE SAR G EWWQ+DI+WYI+  L+ IGLATDVKLPSE Q+   A
Sbjct: 266 SVFSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFLEIIGLATDVKLPSESQRRRMA 303

[Close]
BLAST of Spg015789 vs. TAIR 10
Match: AT3G15870.1 (Fatty acid desaturase family protein )

HSP 1 Score: 286.6 bits (732), Expect = 2.3e-77
Identity = 153/323 (47.37%), Postives = 185/323 (57.28%), Query Frame = 0 

Query: 2   EAPREVEKK---------VAGSRRALWRRKWTFLDIRSATFILLAHLLCIFAPFHFSWAA 61
           EAP  VE           V  ++R+ W R WT  D+      +  HLL + APF+FSW A
Sbjct: 61  EAPDHVESSWRRLLSEVVVVRTKRSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEA 120

Query: 62  LLVAFTLYVVVSVFGMALSYHRNLTHKSFKLPKWLEYLFAYVGVMALQGDPIDWVSTHRD 121
             V F   V ++   + LSYHRNL+H+SF LPKWLEYLFAY GV+A QGDPI+WVS HR 
Sbjct: 121 FWV-FPWLVFINGICITLSYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRY 180

Query: 122 HHQFVETERDPHSPIHGFWFSHLGWTMDSYALTKRVCPKYAEDFQAIDTNILGFCLKYGR 181
           HH+  ET+RDPHSP  GFWFSH+ W  D+                   ++IL  C   G 
Sbjct: 181 HHKHCETQRDPHSPTQGFWFSHMAWIFDT-------------------SSILENC---GG 240

Query: 182 PDNVSDLEGQIFYRFIQKTYLLHPLALGIVLYLMGGFPFLVW------------------ 241
            +NV DL  Q FYRF+Q+T LLH +A   + Y  GG P LVW                  
Sbjct: 241 EENVDDLVRQPFYRFLQRTVLLHMMAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNS 300

Query: 242 ---------------SGNNWLLALLTFGEGWHNNHHAFEYSARHGFEWWQLDITWYIILC 283
                          S NNW +A+LT GEGWHNNHHAFE+SARHG EWWQLDITW +I  
Sbjct: 301 VCHIWGTRAWNTSDFSKNNWWVAILTLGEGWHNNHHAFEFSARHGLEWWQLDITWCLIRF 360

[Close]
BLAST of Spg015789 vs. TAIR 10
Match: AT1G06100.1 (Fatty acid desaturase family protein )

HSP 1 Score: 276.9 bits (707), Expect = 1.8e-74
Identity = 144/308 (46.75%), Postives = 183/308 (59.42%), Query Frame = 0 

Query: 8   EKKVAGSRRALWRRKWTFLDIRSATFILLAHLLCIFAPFHFSWAALLVAFTLYVVVSVFG 67
           +K V   +RA   RKWT  D+  A+ +   HLLC+ APF++ W A      L ++ ++  
Sbjct: 13  KKSVRKEKRAYVLRKWTQFDVGRASTVGTVHLLCLLAPFNYKWEAFRFGIILAILTNLC- 72

Query: 68  MALSYHRNLTHKSFKLPKWLEYLFAYVGVMALQGDPIDWVSTHRDHHQFVETERDPHSPI 127
           +  SYHRNLTH+SFKLPKWLEY FAY  ++ALQGDP+DWVS HR HHQF +++RDPHSPI
Sbjct: 73  ITFSYHRNLTHRSFKLPKWLEYPFAYSALLALQGDPLDWVSIHRFHHQFTDSDRDPHSPI 132

Query: 128 HGFWFSHLGWTMDSYALTKRVCPKYAEDFQAIDTNILGFCLKYGRPDNVSDLEGQIFYRF 187
            GFWFSH+ W  D+  + +                      K GR +NV DL+ Q FYRF
Sbjct: 133 EGFWFSHVLWIFDTDYIRE----------------------KCGRRNNVMDLKQQWFYRF 192

Query: 188 IQKTYLLHPLALGIVLYLMGGFPFLVW--------------------------------- 247
           ++KT +LH LA   ++YL GG P+L W                                 
Sbjct: 193 LKKTLVLHILAFWTLIYLWGGLPYLTWTVGFGGVIGYHGTWLVNSACHICGSQAWQTNDT 252

Query: 248 SGNNWLLALLTFGEGWHNNHHAFEYSARHGFEWWQLDITWYIILCLQAIGLATDVKLPSE 283
           S N W LALLT GE WHNNHHAFE SARHG EW+QLDITWY+I   QA+GLAT+VKLP++
Sbjct: 253 SRNVWWLALLTMGESWHNNHHAFETSARHGLEWYQLDITWYLIWFFQALGLATNVKLPTD 297

[Close]
BLAST of Spg015789 vs. TAIR 10
Match: AT1G06090.1 (Fatty acid desaturase family protein )

HSP 1 Score: 273.9 bits (699), Expect = 1.5e-73
Identity = 144/302 (47.68%), Postives = 177/302 (58.61%), Query Frame = 0 

Query: 9   KKVAGSRRALWRRKWTFLDIRSATFILLAHLLCIFAPFHFSWAALLVAFTLYVVVSVFGM 68
           K V G +RA + RKWT +DI  A+ +   HLLC+ APF++ W AL     L +V S+  +
Sbjct: 14  KAVRGEKRAFFFRKWTRIDIARASAVGAVHLLCLLAPFNYKWEALRFGVILAIVTSL-SI 73

Query: 69  ALSYHRNLTHKSFKLPKWLEYLFAYVGVMALQGDPIDWVSTHRDHHQFVETERDPHSPIH 128
             SYHRNLTHKSFKLPKWLEY FAY  + ALQG PIDWVSTHR HHQF +++RDPHSPI 
Sbjct: 74  TFSYHRNLTHKSFKLPKWLEYPFAYSALFALQGHPIDWVSTHRFHHQFTDSDRDPHSPIE 133

Query: 129 GFWFSHLGWTMDSYALTKRVCPKYAEDFQAIDTNILGFCLKYGRPDNVSDLEGQIFYRFI 188
           GFWFSH+ W  D+  + +                      K G  DNV DL+ Q FYRF+
Sbjct: 134 GFWFSHVFWIFDTSYIRE----------------------KCGGRDNVMDLKQQWFYRFL 193

Query: 189 QKTYLLHPLALGIVLYLMGGFPFL-----------------------VW----------S 248
           + T  LH L    ++YL GG P+L                       +W          S
Sbjct: 194 RNTIGLHILTFWTLVYLWGGLPYLTCGVGVGGTIGYNGTWLINSACHIWGSRAWNTKDTS 253

Query: 249 GNNWLLALLTFGEGWHNNHHAFEYSARHGFEWWQLDITWYIILCLQAIGLATDVKLPSEK 278
            N W L   T GE WHNNHHAFE SARHG EW+Q+D+TWY+I   QA+GLATDVKLP++ 
Sbjct: 254 RNIWWLGPFTMGESWHNNHHAFEASARHGLEWYQVDLTWYLICFFQALGLATDVKLPTDA 292

The following BLAST results are available for this feature:
BLAST of Spg015789 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_008449174.12.0e-11566.04PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplas... [more]
XP_022153644.16.5e-10359.61palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [M... [more]
XP_022153481.18.5e-10358.10acyl-CoA C20 Delta5-desaturase-like isoform X1 [Momordica charantia][more]
XP_022153442.11.5e-10259.80palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [M... [more]
XP_022956260.11.5e-10257.68palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [C... [more]
BLAST of Spg015789 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q949X03.3e-8952.51Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arab... [more]
P0DOW22.9e-7748.48Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1[more]
P0DOW32.9e-7749.49Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1[more]
O657978.3e-7748.50Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=... [more]
Q9FV681.9e-7647.27Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii OX=28973 PE=1 SV=1[more]
BLAST of Spg015789 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A1S3BMC99.5e-11666.04palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1DJP03.2e-10359.61palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1DKS54.1e-10358.10acyl-CoA C20 Delta5-desaturase-like isoform X1 OS=Momordica charantia OX=3673 GN... [more]
A0A6J1DIX07.0e-10359.80palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1GXC17.0e-10357.68palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
BLAST of Spg015789 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G15850.12.3e-9052.51fatty acid desaturase 5 [more]
AT1G06080.15.9e-7848.50delta 9 desaturase 1 [more]
AT3G15870.12.3e-7747.37Fatty acid desaturase family protein [more]
AT1G06100.11.8e-7446.75Fatty acid desaturase family protein [more]
AT1G06090.11.5e-7347.68Fatty acid desaturase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015876Acyl-CoA desaturasePRINTSPR00075FACDDSATRASEcoord: 110..139
score: 51.93
coord: 227..241
score: 60.7
coord: 73..93
score: 30.58
IPR015876Acyl-CoA desaturasePANTHERPTHR11351ACYL-COA DESATURASEcoord: 215..284
IPR015876Acyl-CoA desaturasePANTHERPTHR11351ACYL-COA DESATURASEcoord: 11..214
IPR015876Acyl-CoA desaturaseCDDcd03505Delta9-FADS-likecoord: 50..270
e-value: 3.13539E-62
score: 192.771
IPR005804Fatty acid desaturase domainPFAMPF00487FA_desaturasecoord: 49..214
e-value: 2.4E-9
score: 37.4
NoneNo IPR availablePANTHERPTHR11351:SF87FATTY ACID DESATURASE, TYPE 1, CORE-RELATEDcoord: 11..214
coord: 215..284

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg015789.1Spg015789.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006631 fatty acid metabolic process
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0005789 endoplasmic reticulum membrane
molecular_function GO:0009979 16:0 monogalactosyldiacylglycerol desaturase activity
molecular_function GO:0016717 oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water