Spg015772 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg015772
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Description(-)-germacrene D synthase-like
Locationscaffold6: 40499818 .. 40505531 (-)
RNA-Seq ExpressionSpg015772
SyntenySpg015772
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCAAACTGAGCGTGTGGGTTTCCAGAACTTGGTCTATTCTCCATATCTTTCACTAAAAGGAAAAGAAAATGTCATCTCAAACACTTGTATCAGATTCCATGGCACCATGCAATGCCTCGTTCAATGATAGTGTTCCTCGTAGATCTGCAAACTTTCAACCAAGTTCTTGGGGAGATTATTTCCTTTACTACCATTCCAACCATCCGGTATGTATTTTGGATTAAGATACTATTTTGGTTCCTCTATTCATCTACTTTAACACTCATTCCATCTCGTTTACATACTTTCAAACATTCGTTTATAATTTAAATAAATTTTAAAATTTTTAAAATTAGTACTTACTGAGAGTAATCAATTTAGTCTGTGATTCCAAACTAATGCGAAAGACTAATTTTAAGATTTATTGATAGTACGAGTACTAAATTTATTTATTTGAAAATACCGAAGTTAAAAATAGAACAAGTTTGACCAAAATGATATTTCAAACAATATTTTATTTTCATATTTAAATTTTTGTTGTTTCAATTGAGGAGGCATTGTTTCATATTTCTTTTTTGGTTAAAATTTTATTTTGGTCTTTGAACTTTCATGTTTATTTTATTTTGGTTTCTAAATTTTCAAAATATTTATTTAGTCCCAAACTTGAAAAAGTAATCATTTTAATTCCTAAACTTTTATCACTTGAATTTTTTTAAAAATAACAATTTTGGTTCCTAAACTTTTATATTGTCTATTTGAGTCGGTAAACTTTGTTTGAAAACTAAAACATTTTGATACTTGTATATTATTTTTCACTCAAATTTTTCTTACCTAACACTTTTGTTATTACCTATGTGTTTATGTACATAATTTGTTAAAATCAAAATAGTGATAAGGACGAAATGATTATACTTTTTTAAGTTCCAATACTAGTTAAAATAGACATTTTGAAAGTTTAAGGACTAAAATGAAAGAAACCTAAAGATTTAATTGACCAAACTGAATATCTTAAAAGTTTAAGGACCAAAATGAAATAAATACCGAGAGTTTAGGATAAAAATGGAATTTAAACTTTCCCTTATTTTTCATATATAGAGAACAAAAATGTATGGTAGGTGAAGTCATAAATTTTTTGTTTGTCACTCTTTCCTTTTACTTATAAAAAATATGTGTGATAAATGATAAGTACCAATAGGTCTACTGTACAATAGGTCTTTCTTTCCCCTTTTTAAACTCCTATTGAGGTACATAATATTTTCGGATATGTTTTTTACTTCGACAAAATATTACTTTTGTTTGTGGGACATAAATATCATGTAGCTTAATTTTGTTCGTGGAGTTTAATAGGTCTCTAAACTTATAATTTTGTATCTAATAAATTCTTGACAAATTTAAAATTTTAAATATTAATCGACTTATTAGATATAAATTTGAATTTTTTGCCTAATGAAATCCAAAATTTCAATTTAGTGTCCGGTAGATCTGTGAATTTAAAAAAAAAAATTGAAATCTTAGAGTTTGATAATCATTTGGTTTCTAGTTTTTAAAAATTAAGCTTGTTTTCTTGCAATTTTCTTGTAATGATTTTTTTTATGAAGAAATCTTATAATTTTTTTTTTTTTTTTTTTTTTTTGAAAAAAAATCTTATAATGGTTTTAGGGTGTGTTTGGTTTAACTTTTTAAGTGTTTAATTTTGAAAATAAATCATTTTGAAAAAATTTCTAGTGTTTGGCAACCACTAAAAATGACTTATTAGAAAATGAGATTGATAAATAAATCATTTTGAGAGAAACACTTGAAACCAACTTTTTGAAAAAGACTTTTTAAGTAAACAATAACTATGTATAAAAACATTTGTGAGGGAATGCAACCAAACATATGAGTGTTTAAATTCTAAACAATTTTTATACAAAATGTTTTTTAAAAAATGCATTCTTGAAAAACATTTAAATTAAAATGTATTCCAAACGGACCCTTATCTTTGTTAATGGCACATTATTTGATATGCTGGTCAAATTTCAAAAACAAAAACAAGTTTTTGAAAATTACTTTTTTTGAGTTTTCAAAATTTGACTTGGTTTTTGAAAACATTGAGAGAAGGTAGATAACAAAACCAAAAAATTTATAGGTAGAAATAGTGTTTTTAAGTTTAATTTTCAAAAACCTAATAGTTATGAAATGGGCCTTAAAAATTATGTAATTTTGAAAGTTCAGGAATCAAATAGATATAAATCTCAAAGTTTAAAGACTAAATTTGTAATTTCGTAGAATCAGGAAGATGAGGATATGGTCAGGGAACAACTTCGAAAACTGAAAGAAGAAGTGAGAAGCATGTTGGCAGCTGCTGAAAAATGTTCAGAAAAACTAAGTTTAATTGACTCAATTCAGCGCCTAGGTCTGTCTTACCACTTTGAAGATGAAATTAATGAAATTCTAGAGCCCATGCAAAAGCTTTGCAATGTTGACAATGACGACGATATTTACACTGTTGCTCTACGATTTCGATTGCTTAGACAACAAGGTTACTTCGTTTCTAGTGGTAAGTCTCTCAAACAGAAATTTCAAAGAATTAGGTTAGGTTTGATTTGCAATTTGGATTTTTTTTTTTTTTTGAGTACATCAACATTTGGGGGTGGGGATTCGAATCTCTAACCTCTTGGTCGAGGGTACATGTCAATTAACATTGAGCTAGACTCGCTTTGGTCAGTTTTTGGATTCAAGGAATCAAATGAACTTGACGAATATATCTTTCGTAAATTTGTTTATAAAAATTGTTTTCGAATTCGATTCAAATAGAAGAAATTCTTAAAAAAGAAAAAAAAAAACCGTATGCTTTCCACGTATCTAGATTTTAAATTTTAAAATGCAAAATTACATTAAAAAAAATAATAATTGATAAATACATTGTTTCATTTAATAAACTCATTTCATTTTTGTTGAAATTAAAATGTGTAGGACATAATCCATTATAAATTATATAATATTGTAAAATAATTATAAAATTTACATAGAATATGTTTCTAAACTAGCTGATTAATGATAGTTGAGATCCAACTTAATATTTAGTTGGTAACTACAACAATCAAACACATTTTTACCACTTTTTAAATTGGGATCAAGTTTGTTAATTTGCTATCAAATGCATATTCGAAAACACTGAAAACATGAACTACAACTTTGTTTTTTATTGAATCCCTTATTTTCATTTTTTTTTTCAAATCACCAACCAAACAAACCCTATAGTTTTGTTTTTTTTGACAATATGGGGGTGGGGGATTCGAATCACAGACTTTGTGGTCTTTAGTACCACTCTGTGCCAGTTGAGCTATGCTCTTGTTGGCAACAAACCCTATAGTTTGCTACAAGAAAATTGAGATTGTTTTATATATTTGGTAAAAGTTAAATTGTAATAGGTTTGGTTTCTAAATTTAGTTGTGTTTCACACATTTTTAAAGTTTAAAAAAGTGTCTAATAAGTTCCTAGACAAGCTTTCAATGCAAGCTTTCAATGTCACCTTAATCTTTAAAAAGTGTCTAATAAATTCCTAAATTTTCAATATTATATTCAGTAAATTTGTAAATTTGAAATTTTGTGTTTAATTGGTCTTTGATTTATATAAGACACAATTTAAAAAATTCAAGGACTTATTAGACACAAAATTGGAATTTAAAAAAAAATTGCTAGACACTTTTTAGAGTTCATGAACCTATTCGACACAACATCAAAAATTTAGAAGCTCATTAGATACTTTTAAAGTGAAGAGATTCATTATATATATATAAAATCCTCCAAGTTCAGATATCGAACTTGGAATTTAACCTTTGGTAAATTTCAAACAGAGATATTCAATAAATTTACAAATGCAAGGGGAGACTTCGAAGAATCAATTGTGAAGGATGAAGGCGGTATATTAAGTTTGTATGAGGCATCCCAGTTGAGAATGAAGGGAGAAAATATATTGGATGAAGCATTGGCTTTCACAACTATTCATCTTCAAGCAATGGCCATGGATTCCAAGTCTCCTTTCTCAGATGAAGCTAAATATGCATTGAAGTGGCCAATGTATAAAGCTTTGCCAAGGTTCATGGCTAGGCACTATATCTCTCTTTATGACAACAATCCATTGAAGAATAATGTTTTGCTCACTTTTGCAAAGTTGGACTTCAATTCTCTCCAAAAACTTTACCAGAAAGAAGTGCGTGAATTTTCAAGGTGAAGCTTTTTCATTAACGTTTCTACCATCTTTGAGTTTTTTTTAATCTCTTTTTTTTTTATAAAATATATCTTGTTCTATTTTAGACTTATATTTAGTTAAATGAGCCAAATATAATCTTAAACGTGTTTTATACAGGTGGTTAAAGAATCTGAAGTTGATGGAGCAATTGTGTTTTGCACGAGATAGGGTGGTGGAATGTTATGTCTGGGCATTGGGAATATACTATGAACCGAAATACTCTAGCGCTAGAAGAATATTAGTCAAAACTATTTCTTTTATATCTGTTCTCGATGATTTGTATGATGTCCATGCTACACTGGAGGAACTTCAACTATTCACCAATGCAATCGAAAGGTTTAGTACTTCAACTCTTCGATTCCTCTGATGTTTTAAACTAATATTCTGACTAATATTAAATTCTAGGTGGGATGTTAATTGCATTGAGAAGCTCCCAAATTACATGAAAGGTCTTTATGAAGCTATACTTGAAGTATATCGAGAGATTGAACAAGATATTTGCAAGAACAACAATATTACATTTGCATTTGATTATGCAAAAGAAGCGGTGAATTCTCTTGTAATGAGGTTTCATATATATTTTTCAAGAAAAATATCTAATTTTCTTTCGAGTTTTGTGTGTACAGATGAAAAGGCAATGCAGAGCTTATTTTGTGGAAGCCAAATGGTTCAATGAAGGGTATGTACCAACAATGGAAGAATACATGAAAGTTGCCATCACCTCTGCTGCTTACTACCTTTTTGCTTCAGTTTCATTCCTTGCTTTGGGAAATGTTGCTTCAAAAGAGGTTTTTCAATGGGCTCAAAGTGACCCCATGCTTCTTAAAGCAACTGGAGTAATTGGCAGGCTCCTGAATGACATAGCTTCCCACAAGGTACCCCAAGAAAAAAAATCAATTGAATTGAAAATTGATTCAATCTTTTGATAACGCCCTTGTTTTAATATTACTTTTCTTAGTCGTTGTATATCTTTTTTTTAGTTTGAACAAGAGAGAGGTCATGTGGCTTCGGCCGTTGAATGCTACATGAAACAATATGGAGTGTCGGAAGAAAAGGCCCTAAATGAACTAGAAAACCAAGTGGTAGAAGCATGGAAAGATGTAATAGAAGACTATACGAAGTCTGCCAAATTTCCAAATGTGATCCTTGACTGTGTATTGAATGTTGCTAGACTCTCAGACCTCTTCTATAAGGAGGGAGATGGTTACACATTCGCAGATGGAGAAACCAAACGCTTTATTACCTTAATACTTAAAGACCCTGTGCCAATTTGATCATCCCACTACACAATTGTTTGTGCTTTTTTTTCTTCATCACAACACCTTTTATCGAATAATTATAATTCTATGATACATATACGGTTGTTGAATTACAAGTGTATTTGAGAGTGTTTCTTAGCACAAACTGCTTTTAAGTACTTTTGATAGATGTACTTTTACAAAAAAATTATGTTTATTATAAATTCTTCGTTGCTTGGTTTTAATACTTGGTTAAATTCTAT

mRNA sequence

ATGTCATCTCAAACACTTGTATCAGATTCCATGGCACCATGCAATGCCTCGTTCAATGATAGTGTTCCTCGTAGATCTGCAAACTTTCAACCAAGTTCTTGGGGAGATTATTTCCTTTACTACCATTCCAACCATCCGAATCAGGAAGATGAGGATATGGTCAGGGAACAACTTCGAAAACTGAAAGAAGAAGTGAGAAGCATGTTGGCAGCTGCTGAAAAATGTTCAGAAAAACTAAGTTTAATTGACTCAATTCAGCGCCTAGGTCTGTCTTACCACTTTGAAGATGAAATTAATGAAATTCTAGAGCCCATGCAAAAGCTTTGCAATGTTGACAATGACGACGATATTTACACTGTTGCTCTACGATTTCGATTGCTTAGACAACAAGGTTACTTCGTTTCTAGTGAGATATTCAATAAATTTACAAATGCAAGGGGAGACTTCGAAGAATCAATTGTGAAGGATGAAGGCGGTATATTAAGTTTGTATGAGGCATCCCAGTTGAGAATGAAGGGAGAAAATATATTGGATGAAGCATTGGCTTTCACAACTATTCATCTTCAAGCAATGGCCATGGATTCCAAGTCTCCTTTCTCAGATGAAGCTAAATATGCATTGAAGTGGCCAATGTATAAAGCTTTGCCAAGGTTCATGGCTAGGCACTATATCTCTCTTTATGACAACAATCCATTGAAGAATAATGTTTTGCTCACTTTTGCAAAGTTGGACTTCAATTCTCTCCAAAAACTTTACCAGAAAGAAGTGCGTGAATTTTCAAGGTGGTTAAAGAATCTGAAGTTGATGGAGCAATTGTGTTTTGCACGAGATAGGGTGGTGGAATGTTATGTCTGGGCATTGGGAATATACTATGAACCGAAATACTCTAGCGCTAGAAGAATATTAGTCAAAACTATTTCTTTTATATCTGTTCTCGATGATTTGTATGATGTCCATGCTACACTGGAGGAACTTCAACTATTCACCAATGCAATCGAAAGGTGGGATGTTAATTGCATTGAGAAGCTCCCAAATTACATGAAAGGTCTTTATGAAGCTATACTTGAAGTATATCGAGAGATTGAACAAGATATTTGCAAGAACAACAATATTACATTTGCATTTGATTATGCAAAAGAAGCGGTGAATTCTCTTGTAATGAGAGCTTATTTTGTGGAAGCCAAATGGTTCAATGAAGGGTATGTACCAACAATGGAAGAATACATGAAAGTTGCCATCACCTCTGCTGCTTACTACCTTTTTGCTTCAGTTTCATTCCTTGCTTTGGGAAATGTTGCTTCAAAAGAGGTTTTTCAATGGGCTCAAAGTGACCCCATGCTTCTTAAAGCAACTGGAGTAATTGGCAGGCTCCTGAATGACATAGCTTCCCACAAGTTTGAACAAGAGAGAGGTCATGTGGCTTCGGCCGTTGAATGCTACATGAAACAATATGGAGTGTCGGAAGAAAAGGCCCTAAATGAACTAGAAAACCAAGTGGTAGAAGCATGGAAAGATGTAATAGAAGACTATACGAAGTCTGCCAAATTTCCAAATGTGATCCTTGACTGTGTATTGAATGTTGCTAGACTCTCAGACCTCTTCTATAAGGAGGGAGATGGTTACACATTCGCAGATGGAGAAACCAAACGCTTTATTACCTTAATACTTAAAGACCCTGTGCCAATTTGA

Coding sequence (CDS)

ATGTCATCTCAAACACTTGTATCAGATTCCATGGCACCATGCAATGCCTCGTTCAATGATAGTGTTCCTCGTAGATCTGCAAACTTTCAACCAAGTTCTTGGGGAGATTATTTCCTTTACTACCATTCCAACCATCCGAATCAGGAAGATGAGGATATGGTCAGGGAACAACTTCGAAAACTGAAAGAAGAAGTGAGAAGCATGTTGGCAGCTGCTGAAAAATGTTCAGAAAAACTAAGTTTAATTGACTCAATTCAGCGCCTAGGTCTGTCTTACCACTTTGAAGATGAAATTAATGAAATTCTAGAGCCCATGCAAAAGCTTTGCAATGTTGACAATGACGACGATATTTACACTGTTGCTCTACGATTTCGATTGCTTAGACAACAAGGTTACTTCGTTTCTAGTGAGATATTCAATAAATTTACAAATGCAAGGGGAGACTTCGAAGAATCAATTGTGAAGGATGAAGGCGGTATATTAAGTTTGTATGAGGCATCCCAGTTGAGAATGAAGGGAGAAAATATATTGGATGAAGCATTGGCTTTCACAACTATTCATCTTCAAGCAATGGCCATGGATTCCAAGTCTCCTTTCTCAGATGAAGCTAAATATGCATTGAAGTGGCCAATGTATAAAGCTTTGCCAAGGTTCATGGCTAGGCACTATATCTCTCTTTATGACAACAATCCATTGAAGAATAATGTTTTGCTCACTTTTGCAAAGTTGGACTTCAATTCTCTCCAAAAACTTTACCAGAAAGAAGTGCGTGAATTTTCAAGGTGGTTAAAGAATCTGAAGTTGATGGAGCAATTGTGTTTTGCACGAGATAGGGTGGTGGAATGTTATGTCTGGGCATTGGGAATATACTATGAACCGAAATACTCTAGCGCTAGAAGAATATTAGTCAAAACTATTTCTTTTATATCTGTTCTCGATGATTTGTATGATGTCCATGCTACACTGGAGGAACTTCAACTATTCACCAATGCAATCGAAAGGTGGGATGTTAATTGCATTGAGAAGCTCCCAAATTACATGAAAGGTCTTTATGAAGCTATACTTGAAGTATATCGAGAGATTGAACAAGATATTTGCAAGAACAACAATATTACATTTGCATTTGATTATGCAAAAGAAGCGGTGAATTCTCTTGTAATGAGAGCTTATTTTGTGGAAGCCAAATGGTTCAATGAAGGGTATGTACCAACAATGGAAGAATACATGAAAGTTGCCATCACCTCTGCTGCTTACTACCTTTTTGCTTCAGTTTCATTCCTTGCTTTGGGAAATGTTGCTTCAAAAGAGGTTTTTCAATGGGCTCAAAGTGACCCCATGCTTCTTAAAGCAACTGGAGTAATTGGCAGGCTCCTGAATGACATAGCTTCCCACAAGTTTGAACAAGAGAGAGGTCATGTGGCTTCGGCCGTTGAATGCTACATGAAACAATATGGAGTGTCGGAAGAAAAGGCCCTAAATGAACTAGAAAACCAAGTGGTAGAAGCATGGAAAGATGTAATAGAAGACTATACGAAGTCTGCCAAATTTCCAAATGTGATCCTTGACTGTGTATTGAATGTTGCTAGACTCTCAGACCTCTTCTATAAGGAGGGAGATGGTTACACATTCGCAGATGGAGAAACCAAACGCTTTATTACCTTAATACTTAAAGACCCTGTGCCAATTTGA

Protein sequence

MSSQTLVSDSMAPCNASFNDSVPRRSANFQPSSWGDYFLYYHSNHPNQEDEDMVREQLRKLKEEVRSMLAAAEKCSEKLSLIDSIQRLGLSYHFEDEINEILEPMQKLCNVDNDDDIYTVALRFRLLRQQGYFVSSEIFNKFTNARGDFEESIVKDEGGILSLYEASQLRMKGENILDEALAFTTIHLQAMAMDSKSPFSDEAKYALKWPMYKALPRFMARHYISLYDNNPLKNNVLLTFAKLDFNSLQKLYQKEVREFSRWLKNLKLMEQLCFARDRVVECYVWALGIYYEPKYSSARRILVKTISFISVLDDLYDVHATLEELQLFTNAIERWDVNCIEKLPNYMKGLYEAILEVYREIEQDICKNNNITFAFDYAKEAVNSLVMRAYFVEAKWFNEGYVPTMEEYMKVAITSAAYYLFASVSFLALGNVASKEVFQWAQSDPMLLKATGVIGRLLNDIASHKFEQERGHVASAVECYMKQYGVSEEKALNELENQVVEAWKDVIEDYTKSAKFPNVILDCVLNVARLSDLFYKEGDGYTFADGETKRFITLILKDPVPI
Homology
BLAST of Spg015772 vs. NCBI nr
Match: XP_038877743.1 (LOW QUALITY PROTEIN: (-)-germacrene D synthase-like [Benincasa hispida])

HSP 1 Score: 841.6 bits (2173), Expect = 3.8e-240
Identity = 425/565 (75.22%), Postives = 488/565 (86.37%), Query Frame = 0

Query: 1   MSSQTLVSDSMAPCNASFNDSVPRRSANFQPSSWGDYFLYYHSNHPNQEDEDMVREQLRK 60
           MS Q +VSDSM P N S +  VPRRSANFQPS+WGD+FL Y  N    E+E+MV +Q+++
Sbjct: 1   MSFQIVVSDSMTPNNDSLSHGVPRRSANFQPSAWGDHFLSYRYN--TLEEEEMVMKQVQE 60

Query: 61  LKEEVRSMLAAAEKCSEKLSLIDSIQRLGLSYHFEDEINEILEPMQKLCNVD-NDDDIYT 120
            KEEVR M   AEK SEKLSLIDSIQRLGLSYHF+DE +E+++ +Q   NVD  D+D+Y 
Sbjct: 61  YKEEVRRMFGVAEKYSEKLSLIDSIQRLGLSYHFKDESSEVIDYIQNPTNVDEEDEDLYI 120

Query: 121 VALRFRLLRQQGYFVSSEIFNKFTNARGDFEESIVKDEGGILSLYEASQLRMKGENILDE 180
            ALRFRLLRQQG+FVS +IFNKFTN  GDF+ESIVKD  G +SLYEAS LRMKGENILDE
Sbjct: 121 TALRFRLLRQQGFFVSCDIFNKFTNEMGDFKESIVKDGAGXISLYEASHLRMKGENILDE 180

Query: 181 ALAFTTIHLQAMAMDSKSPFSDEAKYALKWPMYKALPRFMARHYISLYDNNPLK-NNVLL 240
           ALAFTT  L+AMAMDS SPFS+EAKYALKWP+YKA+PRFM RHYISLY NN LK NNVLL
Sbjct: 181 ALAFTTTQLKAMAMDSSSPFSEEAKYALKWPIYKAMPRFMNRHYISLYQNNALKNNNVLL 240

Query: 241 TFAKLDFNSLQKLYQKEVREFSRWLKNLKLMEQLCFARDRVVECYVWALGIYYEPKYSSA 300
            FAKLDFN+LQKLYQKE+ EFSRW K+LKL  QL FARDR VECYVWALGI+YEPKYS+A
Sbjct: 241 KFAKLDFNTLQKLYQKELGEFSRWWKDLKLRMQLPFARDRGVECYVWALGIFYEPKYSNA 300

Query: 301 RRILVKTISFISVLDDLYDVHATLEELQLFTNAIERWDVNCIEKLPNYMKGLYEAILEVY 360
           R IL K I+FIS+LDD+YDV+ATLEELQLFTN IERWDVNCI+ LPNYMKGLYEAILEVY
Sbjct: 301 RTILAKVIAFISLLDDMYDVYATLEELQLFTNLIERWDVNCIKMLPNYMKGLYEAILEVY 360

Query: 361 REIEQDICKNNNITFAFDYAKEAVNSLVMRAYFVEAKWFNEGYVPTMEEYMKVAITSAAY 420
            EIEQDICK+NNI FAFDYAKE +  +  RAY +EAKWFN+ Y+PTMEEYMKVAI SAAY
Sbjct: 361 GEIEQDICKDNNIPFAFDYAKETM-KMQCRAYLMEAKWFNQRYIPTMEEYMKVAIISAAY 420

Query: 421 YLFASVSFLALGNVASKEVFQWAQSDPMLLKATGVIGRLLNDIASHKFEQERGHVASAVE 480
           Y+ AS+SF+ALGNVASKE+F+WAQ+DPML+KATGVIGRLLNDIASHKFEQERGHVASA+E
Sbjct: 421 YVVASISFIALGNVASKEIFEWAQTDPMLIKATGVIGRLLNDIASHKFEQERGHVASAIE 480

Query: 481 CYMKQYGVSEEKALNELENQVVEAWKDVIEDYTKSAK-FPNVILDCVLNVARLSDLFYKE 540
           CYMKQYGVSEE+A+ EL+ QVV+AWKDVIED+T+S++ FP VI DC+LN+ARL+DLFYK+
Sbjct: 481 CYMKQYGVSEEEAVVELKLQVVKAWKDVIEDHTESSQLFPTVICDCILNIARLTDLFYKD 540

Query: 541 GDGYTFADGETKRFITLILKDPVPI 563
           GDGYTFADGE K FIT +L DPVPI
Sbjct: 541 GDGYTFADGEIKHFITFMLIDPVPI 562

BLAST of Spg015772 vs. NCBI nr
Match: XP_022143456.1 ((-)-germacrene D synthase-like isoform X1 [Momordica charantia])

HSP 1 Score: 833.2 bits (2151), Expect = 1.4e-237
Identity = 419/558 (75.09%), Postives = 484/558 (86.74%), Query Frame = 0

Query: 6   LVSDSMAPCNASFNDSVPRRSANFQPSSWGDYFLYYHSNHPNQEDEDMVREQLRKLKEEV 65
           +VS+  AP N   ND V RRSANFQPS WGDYFL+   +H N + +DMV ++L+KLKEEV
Sbjct: 5   VVSNLTAPDNDPSNDGVLRRSANFQPSVWGDYFLF---SHINTDMDDMVGKKLQKLKEEV 64

Query: 66  RSMLAAAEKCSEKLSLIDSIQRLGLSYHFEDEINEILEPMQKLCNV-DNDDDIYTVALRF 125
           RSMLAAAEKCS+KLSLIDSIQRLGLS+HFE+EINEILE MQ   ++ D+D+D+  VALRF
Sbjct: 65  RSMLAAAEKCSKKLSLIDSIQRLGLSHHFEEEINEILEQMQNXRSLNDDDEDVDIVALRF 124

Query: 126 RLLRQQGYFVSSEIFNKFTNARGDFEESIVKDEGGILSLYEASQLRMKGENILDEALAFT 185
           RLLRQ+GY VS EIFN+F N  GDF+ES+VKDE GILSLYEAS LR+ GE+ILD+ALAFT
Sbjct: 125 RLLRQRGYSVSCEIFNRFINGNGDFKESMVKDECGILSLYEASHLRINGEDILDKALAFT 184

Query: 186 TIHLQAMAMDSKSPFSDEAKYALKWPMYKALPRFMARHYISLYDNNPLKNNVLLTFAKLD 245
           T HLQAMAMDS SPF++EAKYALKWP+YKALPRFM RHYISLYDN+PLK+NVLLTF+KLD
Sbjct: 185 TTHLQAMAMDSSSPFAEEAKYALKWPIYKALPRFMTRHYISLYDNDPLKSNVLLTFSKLD 244

Query: 246 FNSLQKLYQKEVREFSRWLKNLKLMEQLCFARDRVVECYVWALGIYYEPKYSSARRILVK 305
           FN+LQKLYQKE+ EFSRW K+LKLMEQ  FARDRVVECY W LGIYYEPKYS+ARRILVK
Sbjct: 245 FNALQKLYQKELVEFSRWWKDLKLMEQFSFARDRVVECYFWTLGIYYEPKYSNARRILVK 304

Query: 306 TISFISVLDDLYDVHATLEELQLFTNAIERWDVNCIEKLPNYMKGLYEAILEVYREIEQD 365
            I+FIS+LDD+ D +ATL+ELQLFT+AIERWD+N I+KLPNYMK + EAIL+VYRE+EQD
Sbjct: 305 VIAFISLLDDMXDAYATLDELQLFTDAIERWDINGIDKLPNYMKVIXEAILQVYREVEQD 364

Query: 366 ICKNNNITFAFDYAKEAVNSLVMRAYFVEAKWFNEGYVPTMEEYMKVAITSAAYYLFASV 425
           I K+NNI F  D AKE++     RAY VEAKWF++G+VP+MEEYM+VAI SAAYYLFASV
Sbjct: 365 IGKDNNILFGLDCAKESMKR-QCRAYLVEAKWFHKGHVPSMEEYMEVAIVSAAYYLFASV 424

Query: 426 SFLALGNVASKEVFQWAQSDPMLLKATGVIGRLLNDIASHKFEQERGHVASAVECYMKQY 485
           SFL +G+VASKE FQW  SDPMLL ATGVIGRL+NDIASHKFEQERGHVASAVECYMKQY
Sbjct: 425 SFLGMGDVASKEAFQWVMSDPMLLNATGVIGRLVNDIASHKFEQERGHVASAVECYMKQY 484

Query: 486 GVSEEKALNELENQVVEAWKDVIEDYTKSAKFPNVILDCVLNVARLSDLFYKEGDGYTFA 545
            VSEE+A+ ELE QV++AWKD+ EDY KS +FPN I+ CV NVARLSDLFYKEGDGYTF 
Sbjct: 485 EVSEEEAVGELEKQVIKAWKDITEDYMKSTRFPNAIVACVTNVARLSDLFYKEGDGYTFP 544

Query: 546 DGETKRFITLILKDPVPI 563
           DGET+RFIT ILKDPVP+
Sbjct: 545 DGETRRFITSILKDPVPL 558

BLAST of Spg015772 vs. NCBI nr
Match: KGN55986.2 (hypothetical protein Csa_011696 [Cucumis sativus])

HSP 1 Score: 818.1 bits (2112), Expect = 4.5e-233
Identity = 408/566 (72.08%), Postives = 485/566 (85.69%), Query Frame = 0

Query: 1   MSSQTLVSDSMAPCN-ASFNDSVPRRSANFQPSSWGDYFLYYHSNHPNQEDEDMVREQLR 60
           +SSQ +VSDS    +  + + +V RR ANFQP++WGDYFL +H N+   EDE+MV +Q+ 
Sbjct: 34  ISSQIVVSDSTDMLHKETLSHAVVRRYANFQPTAWGDYFLSHHYNN-TMEDEEMVMKQVE 93

Query: 61  KLKEEVRSMLAAAEKCSEKLSLIDSIQRLGLSYHFEDEINEILEPMQKLCNVDNDD-DIY 120
           ++ E VRSM   A+KCSEKLSLID IQRLGL Y+FEDEINE+L  M    N+D +D D+Y
Sbjct: 94  EMTEAVRSMFGDAKKCSEKLSLIDLIQRLGLYYYFEDEINEVLGLMHNASNLDEEDVDLY 153

Query: 121 TVALRFRLLRQQGYFVSSEIFNKFTNARGDFEESIVKDEGGILSLYEASQLRMKGENILD 180
           T+ALRFRLLRQ+G+FVS EIFNK+TN  GDF+ESI KDE G+LSLYEAS LRMKGENILD
Sbjct: 154 TMALRFRLLRQKGFFVSCEIFNKYTNESGDFKESITKDEVGLLSLYEASHLRMKGENILD 213

Query: 181 EALAFTTIHLQAMAMDSKSPFSDEAKYALKWPMYKALPRFMARHYISLYDNNPLKNNVLL 240
           +ALAFTT  LQA+AMDS SPFS E K++LKWP+YKA+PRFM+RHYISLY NNPLK+NVLL
Sbjct: 214 KALAFTTTQLQAIAMDSNSPFSQEVKFSLKWPIYKAMPRFMSRHYISLYQNNPLKDNVLL 273

Query: 241 TFAKLDFNSLQKLYQKEVREFSRWLKNLKLMEQLCFARDRVVECYVWALGIYYEPKYSSA 300
           TFAKLD+NSLQKLYQKE+ EFSRW K++ L EQLCFARDR VECY WA+G+YYEPKYSS 
Sbjct: 274 TFAKLDYNSLQKLYQKELGEFSRWWKDMMLREQLCFARDRAVECYTWAMGVYYEPKYSSG 333

Query: 301 RRILVKTISFISVLDDLYDVHATLEELQLFTNAIERWDVNCIEKLPNYMKGLYEAILEVY 360
           R +L K I+F+S+LDD+YD +AT EELQLFT++IERWDVNCIE LPNYMKGLYE ILEVY
Sbjct: 334 RILLAKVIAFLSILDDMYDAYATFEELQLFTHSIERWDVNCIEMLPNYMKGLYETILEVY 393

Query: 361 REIEQDICKNNNITFAFDYAKEAVNSLVMRAYFVEAKWFNEGYVPTMEEYMKVAITSAAY 420
             IE+DICK +NI FAFD AKEA+ +   RA+F+EAKWFNEGY+PT+EEYMKV+ITSAAY
Sbjct: 394 EGIEKDICKVDNIPFAFDCAKEAMKN-QCRAFFMEAKWFNEGYIPTVEEYMKVSITSAAY 453

Query: 421 YLFASVSFLALG-NVASKEVFQWAQSDPMLLKATGVIGRLLNDIASHKFEQERGHVASAV 480
           Y+FAS+SFL +G N ASK+V++WAQ+DP+LL+AT VIGR +NDIASHKFEQERGHVASAV
Sbjct: 454 YVFASISFLTIGNNAASKQVYEWAQTDPILLRATSVIGRFVNDIASHKFEQERGHVASAV 513

Query: 481 ECYMKQYGVSEEKALNELENQVVEAWKDVIEDYTKSAK-FPNVILDCVLNVARLSDLFYK 540
           ECYMKQYGVSEE+A+ EL+ QVV AWKD+IEDY KS+K FP+ ILD VLNVARLSDLFYK
Sbjct: 514 ECYMKQYGVSEEEAVTELQKQVVNAWKDIIEDYMKSSKLFPSFILDHVLNVARLSDLFYK 573

Query: 541 EGDGYTFADGETKRFITLILKDPVPI 563
           E DGYTFADGETKRFITL+LK+P+PI
Sbjct: 574 EEDGYTFADGETKRFITLMLKNPMPI 597

BLAST of Spg015772 vs. NCBI nr
Match: XP_004151461.3 ((-)-germacrene D synthase [Cucumis sativus])

HSP 1 Score: 813.9 bits (2101), Expect = 8.5e-232
Identity = 401/549 (73.04%), Postives = 475/549 (86.52%), Query Frame = 0

Query: 17  SFNDSVPRRSANFQPSSWGDYFLYYHSNHPNQEDEDMVREQLRKLKEEVRSMLAAAEKCS 76
           + + +V RR ANFQP++WGDYFL +H N+   EDE+MV +Q+ ++ E VRSM   A+KCS
Sbjct: 6   TLSHAVVRRYANFQPTAWGDYFLSHHYNN-TMEDEEMVMKQVEEMTEAVRSMFGDAKKCS 65

Query: 77  EKLSLIDSIQRLGLSYHFEDEINEILEPMQKLCNVDNDD-DIYTVALRFRLLRQQGYFVS 136
           EKLSLID IQRLGL Y+FEDEINE+L  M    N+D +D D+YT+ALRFRLLRQ+G+FVS
Sbjct: 66  EKLSLIDLIQRLGLYYYFEDEINEVLGLMHNASNLDEEDVDLYTMALRFRLLRQKGFFVS 125

Query: 137 SEIFNKFTNARGDFEESIVKDEGGILSLYEASQLRMKGENILDEALAFTTIHLQAMAMDS 196
            EIFNK+TN  GDF+ESI KDE G+LSLYEAS LRMKGENILD+ALAFTT  LQA+AMDS
Sbjct: 126 CEIFNKYTNESGDFKESITKDEVGLLSLYEASHLRMKGENILDKALAFTTTQLQAIAMDS 185

Query: 197 KSPFSDEAKYALKWPMYKALPRFMARHYISLYDNNPLKNNVLLTFAKLDFNSLQKLYQKE 256
            SPFS E K++LKWP+YKA+PRFM+RHYISLY NNPLK+NVLLTFAKLD+NSLQKLYQKE
Sbjct: 186 NSPFSQEVKFSLKWPIYKAMPRFMSRHYISLYQNNPLKDNVLLTFAKLDYNSLQKLYQKE 245

Query: 257 VREFSRWLKNLKLMEQLCFARDRVVECYVWALGIYYEPKYSSARRILVKTISFISVLDDL 316
           + EFSRW K++ L EQLCFARDR VECY WA+G+YYEPKYSS R +L K I+F+S+LDD+
Sbjct: 246 LGEFSRWWKDMMLREQLCFARDRAVECYTWAMGVYYEPKYSSGRILLAKVIAFLSILDDM 305

Query: 317 YDVHATLEELQLFTNAIERWDVNCIEKLPNYMKGLYEAILEVYREIEQDICKNNNITFAF 376
           YD +AT EELQLFT++IERWDVNCIE LPNYMKGLYE ILEVY  IE+DICK +NI FAF
Sbjct: 306 YDAYATFEELQLFTHSIERWDVNCIEMLPNYMKGLYETILEVYEGIEKDICKVDNIPFAF 365

Query: 377 DYAKEAVNSLVMRAYFVEAKWFNEGYVPTMEEYMKVAITSAAYYLFASVSFLALG-NVAS 436
           D AKEA+ +   RA+F+EAKWFNEGY+PT+EEYMKV+ITSAAYY+FAS+SFL +G N AS
Sbjct: 366 DCAKEAMKN-QCRAFFMEAKWFNEGYIPTVEEYMKVSITSAAYYVFASISFLTIGNNAAS 425

Query: 437 KEVFQWAQSDPMLLKATGVIGRLLNDIASHKFEQERGHVASAVECYMKQYGVSEEKALNE 496
           K+V++WAQ+DP+LL+AT VIGR +NDIASHKFEQERGHVASAVECYMKQYGVSEE+A+ E
Sbjct: 426 KQVYEWAQTDPILLRATSVIGRFVNDIASHKFEQERGHVASAVECYMKQYGVSEEEAVTE 485

Query: 497 LENQVVEAWKDVIEDYTKSAK-FPNVILDCVLNVARLSDLFYKEGDGYTFADGETKRFIT 556
           L+ QVV AWKD+IEDY KS+K FP+ ILD VLNVARLSDLFYKE DGYTFADGETKRFIT
Sbjct: 486 LQKQVVNAWKDIIEDYMKSSKLFPSFILDHVLNVARLSDLFYKEEDGYTFADGETKRFIT 545

Query: 557 LILKDPVPI 563
           L+LK+P+PI
Sbjct: 546 LMLKNPMPI 552

BLAST of Spg015772 vs. NCBI nr
Match: XP_038903578.1 (LOW QUALITY PROTEIN: (-)-germacrene D synthase-like [Benincasa hispida])

HSP 1 Score: 811.2 bits (2094), Expect = 5.5e-231
Identity = 404/496 (81.45%), Postives = 449/496 (90.52%), Query Frame = 0

Query: 68  MLAAAEKCSEKLSLIDSIQRLGLSYHFEDEINEILEPMQKLCNVDNDDDIYTVALRFRLL 127
           M AAA+  SE+LSLIDSIQRLG+SYHFEDEINEILE MQKL  VD D+D+Y +ALRF LL
Sbjct: 1   MFAAADITSERLSLIDSIQRLGVSYHFEDEINEILEHMQKLSKVDGDEDLYIIALRFXLL 60

Query: 128 RQQGYFVSSEIFNKFTNARGDFEESIVKDEGGILSLYEASQLRMKGENILDEALAFTTIH 187
           RQQGY+VS EIFNKFTN  GDF+ESIVKDE GILSLYEAS LR+KGENILDEALAFTT  
Sbjct: 61  RQQGYYVSCEIFNKFTNENGDFKESIVKDECGILSLYEASHLRIKGENILDEALAFTTTQ 120

Query: 188 LQAMAMDSKSPFSDEAKYALKWPMYKALPRFMARHYISLYDNNPLKNN-VLLTFAKLDFN 247
           L+AMAMDS SPFS+EAKYALKWP++KALPRFM RHYISLY  NPLKNN VLLTFAKLD+N
Sbjct: 121 LKAMAMDSSSPFSEEAKYALKWPIHKALPRFMTRHYISLYTKNPLKNNDVLLTFAKLDYN 180

Query: 248 SLQKLYQKEVREFSRWLKNLKLMEQLCFARDRVVECYVWALGIYYEPKYSSARRILVKTI 307
           SLQK YQKEV EFSRW K+LKL EQL F+RDRV+ECY+WALGIYYEP+YS+ARRILVK I
Sbjct: 181 SLQKFYQKEVGEFSRWWKDLKLREQLYFSRDRVMECYIWALGIYYEPQYSNARRILVKVI 240

Query: 308 SFISVLDDLYDVHATLEELQLFTNAIERWDVNCIEKLPNYMKGLYEAILEVYREIEQDIC 367
           + ISVLDD+YDV+ATL+ELQ FT AIERWDVNCI+KLPNYMKGLYEAILEVY EIE DIC
Sbjct: 241 ALISVLDDMYDVYATLDELQPFTKAIERWDVNCIDKLPNYMKGLYEAILEVYGEIE-DIC 300

Query: 368 KNNNITFAFDYAKEAVNSLVMRAYFVEAKWFNEGYVPTMEEYMKVAITSAAYYLFASVSF 427
           K+NNI FAFDYAKEA+     RAYF+EAKWFNEGY+PTMEEYMKVA+ SAAYYLFASVSF
Sbjct: 301 KDNNIPFAFDYAKEAMKR-QCRAYFMEAKWFNEGYIPTMEEYMKVALISAAYYLFASVSF 360

Query: 428 LALGNVASKEVFQWAQSDPMLLKATGVIGRLLNDIASHKFEQERGHVASAVECYMKQYGV 487
           LALGN+AS+EVF+WAQ+DPMLLKATGVIGRLLNDIASHKFEQ+RGHVASA+ECYMK+YGV
Sbjct: 361 LALGNIASREVFEWAQTDPMLLKATGVIGRLLNDIASHKFEQKRGHVASAIECYMKEYGV 420

Query: 488 SEEKALNELENQVVEAWKDVIEDYTKSAKFPNVILDCVLNVARLSDLFYKEGDGYTFADG 547
           SEE+AL +L+ +V  AWKDV +DY KS+KFPNVILDCVLNVARLSDLFYKEGDGYTFADG
Sbjct: 421 SEEEALTKLDKEVERAWKDVTKDYIKSSKFPNVILDCVLNVARLSDLFYKEGDGYTFADG 480

Query: 548 ETKRFITLILKDPVPI 563
           ETK+FITL+L  PVPI
Sbjct: 481 ETKQFITLLLIHPVPI 494

BLAST of Spg015772 vs. ExPASy Swiss-Prot
Match: Q6Q3H3 ((-)-germacrene D synthase OS=Vitis vinifera OX=29760 GN=VIT_19s0014g04930 PE=1 SV=1)

HSP 1 Score: 553.5 bits (1425), Expect = 2.7e-156
Identity = 283/553 (51.18%), Postives = 387/553 (69.98%), Query Frame = 0

Query: 11  MAPCNASFNDSVPRRSANFQPSSWGDYFLYYHSNHPNQEDEDMVREQLRKLKEEVRSMLA 70
           +AP + + +  V RR ANF PS WGD+FL Y S   N +D   +++ +++LKEEVR ML 
Sbjct: 10  LAP-SKNLSPEVGRRCANFHPSIWGDHFLSYASEFTNTDDH--LKQHVQQLKEEVRKMLM 69

Query: 71  AAEKCS-EKLSLIDSIQRLGLSYHFEDEINEILEPMQKLCNVDNDDDIYTVALRFRLLRQ 130
           AA+  S +KL LID+IQRLG++YHFE EI+E+L+ M     V  ++D+YT +LRFRLLRQ
Sbjct: 70  AADDDSAQKLLLIDAIQRLGVAYHFESEIDEVLKHMFDGSVVSAEEDVYTASLRFRLLRQ 129

Query: 131 QGYFVSSEIFNKFTNARGDFEESIVKDEGGILSLYEASQLRMKGENILDEALAFTTIHLQ 190
           QGY VS ++FN F +  G+F+ES+  D  G+LSLYEA+  R+ GE+ILDEALAFTT HLQ
Sbjct: 130 QGYHVSCDLFNNFKDNEGNFKESLSSDVRGMLSLYEATHFRVHGEDILDEALAFTTTHLQ 189

Query: 191 AMAMDSKSPFSDEAKYALKWPMYKALPRFMARHYISLYDNNPLKNNVLLTFAKLDFNSLQ 250
           +    S +P +++  +ALK P+ K LPR  ARHY S+Y  +   N  LL  AKLDFN LQ
Sbjct: 190 SATKHSSNPLAEQVVHALKQPIRKGLPRLEARHYFSVYQADDSHNKALLKLAKLDFNLLQ 249

Query: 251 KLYQKEVREFSRWLKNLKLMEQLCFARDRVVECYVWALGIYYEPKYSSARRILVKTISFI 310
           KL+QKE+ + S W K+L    +L FARDRVVECY W LG+Y+EP++  ARRIL K I+  
Sbjct: 250 KLHQKELSDISAWWKDLDFAHKLPFARDRVVECYFWILGVYFEPQFFFARRILTKVIAMT 309

Query: 311 SVLDDLYDVHATLEELQLFTNAIERWDVNCIEKLPNYMKGLYEAILEVYREIEQDICKNN 370
           S++DD+YDV+ TLEEL+LFT A+ERWD++ I++LP YM+  Y+A+L VY EIE+++ K  
Sbjct: 310 SIIDDIYDVYGTLEELELFTEAVERWDISAIDQLPEYMRVCYQALLYVYSEIEEEMAKEG 369

Query: 371 NITFAFDYAKEAVNSLVMRAYFVEAKWFNEGYVPTMEEYMKVAITSAAYYLFASVSFLAL 430
             ++   YAKEA+ + V RAY+ EAKW     +PTMEEYM VA+ ++AY + A+ SF+ +
Sbjct: 370 R-SYRLYYAKEAMKNQV-RAYYEEAKWLQVQQIPTMEEYMPVALVTSAYSMLATTSFVGM 429

Query: 431 GNVASKEVFQWAQSDPMLLKATGVIGRLLNDIASHKFEQERGHVASAVECYMKQYGVSEE 490
           G+  +KE F W  S P +++A+ ++ RL++D+  HKFEQ+RGHVASAVECYMKQ+G SE+
Sbjct: 430 GDAVTKESFDWIFSKPKIVRASAIVCRLMDDMVFHKFEQKRGHVASAVECYMKQHGASEQ 489

Query: 491 KALNELENQVVEAWKDVIEDYTKSAKFPNVILDCVLNVARLSDLFYKEGDGYTFADGETK 550
           +  NE    V EAWKD+ E+       P  IL  VLN+AR+ D+ YK  DGYT      K
Sbjct: 490 ETPNEFPQPVREAWKDINEECLIPTAVPMPILMRVLNLARVIDVIYKNEDGYTHFGAVLK 549

Query: 551 RFITLILKDPVPI 563
            F+T +L DPVPI
Sbjct: 550 DFVTSMLIDPVPI 557

BLAST of Spg015772 vs. ExPASy Swiss-Prot
Match: B9SCB6 (Probable terpene synthase 2 OS=Ricinus communis OX=3988 GN=TPS2 PE=3 SV=1)

HSP 1 Score: 522.3 bits (1344), Expect = 6.7e-147
Identity = 265/560 (47.32%), Postives = 381/560 (68.04%), Query Frame = 0

Query: 1   MSSQTLVSDSMAPCNASFNDSVPRRSANFQPSSWGDYFLYYHSNHPNQEDEDMVREQLRK 60
           MS+QTL   ++ P       +  R  A+F P+ WGD FL   +   + ED+  + +Q+ +
Sbjct: 1   MSAQTLAISNLKP-------NTTRHLASFHPNIWGDRFLSCAAESTDIEDD--MEQQVER 60

Query: 61  LKEEVRSMLAAAEKCSEKLSLIDSIQRLGLSYHFEDEINEILEPMQKL-CNVDNDDDIYT 120
           LKEEV+ M+A+A++ S+ L+LID +QRLG+SYHFE EI E L+ +  +  + D DDD+++
Sbjct: 61  LKEEVKKMIASADEPSQILNLIDLLQRLGVSYHFEKEIEEALQQVLNMNSDSDKDDDLHS 120

Query: 121 VALRFRLLRQQGYFVSSEIFNKFTNARGDFEESIVKDEGGILSLYEASQLRMKGENILDE 180
           VALRFRLLR+QG  VS ++FNKF +  G F +++  D  G+LSLYEA+  R+ GE ILD+
Sbjct: 121 VALRFRLLREQGLNVSCDVFNKFRDRNGHFIQTLKTDLQGMLSLYEAAHFRVHGEGILDD 180

Query: 181 ALAFTTIHLQAMAMDSKSPFSDEAKYALKWPMYKALPRFMARHYISLYDNNPLKNNVLLT 240
           ALAFTT +L+++  +   P + +    L+ P+ K+L R  ARH+IS+Y  +   N VLLT
Sbjct: 181 ALAFTTTYLESIVPNLSPPLAAQISRTLRQPLRKSLARVEARHFISIYQEDTSHNEVLLT 240

Query: 241 FAKLDFNSLQKLYQKEVREFSRWLKNLKLMEQLCFARDRVVECYVWALGIYYEPKYSSAR 300
           FAKLDFN LQKL+QKE++  S W K+L  + +L F RDRVVE Y W LG+Y+EP+Y  AR
Sbjct: 241 FAKLDFNLLQKLHQKELKYISLWWKDLDFVNKLPFTRDRVVEGYFWILGVYFEPQYHRAR 300

Query: 301 RILVKTISFISVLDDLYDVHATLEELQLFTNAIERWDVNCIEKLPNYMKGLYEAILEVYR 360
           + + K I+ +SV+DD+YD + TLEEL +FT+AI RWD++CI++LP YMK  Y+A+L VY 
Sbjct: 301 KFVTKVINVVSVIDDIYDAYGTLEELVVFTDAINRWDIDCIDQLPEYMKVCYKALLNVYE 360

Query: 361 EIEQDICKNNNITFAFDYAKEAVNSLVMRAYFVEAKWFNEGYVPTMEEYMKVAITSAAYY 420
           EIE+ + +    ++   YAKEA+  LV +AY VEA W N+ YVPTM+EYM +A+ S AY 
Sbjct: 361 EIERALSEQGR-SYRLHYAKEAMKKLV-QAYLVEANWMNKNYVPTMDEYMSIALVSCAYP 420

Query: 421 LFASVSFLALGNVASKEVFQWAQSDPMLLKATGVIGRLLNDIASHKFEQERGHVASAVEC 480
           L    SF+ +G++A+KEVF WA +DP +++   +I RL++DI SH+FEQ+RGH+AS+VEC
Sbjct: 421 LLTVTSFVGMGDIATKEVFDWASNDPKIVRVASIICRLMDDIVSHEFEQKRGHIASSVEC 480

Query: 481 YMKQYGVSEEKALNELENQVVEAWKDVIEDYTKSAKFPNVILDCVLNVARLSDLFYKEGD 540
           YMKQ GVSEE   +E   Q+V+AWKD+ E++ +    P      V+N AR+ D  YK+ D
Sbjct: 481 YMKQNGVSEEATRDEFNKQIVDAWKDINEEHLQPNYVPMPFRTRVVNSARIMDYLYKDDD 540

Query: 541 GYTFADGETKRFITLILKDP 560
            YT      K  +  +L DP
Sbjct: 541 EYTHVGELMKGSVAALLIDP 549

BLAST of Spg015772 vs. ExPASy Swiss-Prot
Match: B9SBV6 (Probable terpene synthase 3 OS=Ricinus communis OX=3988 GN=TPS3 PE=3 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 2.2e-145
Identity = 266/553 (48.10%), Postives = 374/553 (67.63%), Query Frame = 0

Query: 13  PCNASFNDSVPRRSANFQPSSWGDYFLYYHSNHPNQEDEDMVREQLRKLKEEVRSM-LAA 72
           P  A+   +  RR+A F PS WGDYF+    N P+ +   + +EQ + L+EEVR M ++ 
Sbjct: 4   PVQAAPQRAQVRRTAQFHPSVWGDYFI---KNAPDNKMVSIWKEQAKVLEEEVRRMIISE 63

Query: 73  AEKCSEKLSLIDSIQRLGLSYHFEDEINEILEPMQKLCNVDNDDDIYTVALRFRLLRQQG 132
            +K S KL LID IQRLG++YHFE+EI E++E  Q   N D+D+D+Y +ALRFRLLRQQG
Sbjct: 64  TQKPSGKLKLIDVIQRLGIAYHFEEEIGEVIE--QVYSNYDDDEDLYDIALRFRLLRQQG 123

Query: 133 YFVSSEIFNKFTNARGDFEESIVKDEGGILSLYEASQLRMKGENILDEALAFTTIHLQAM 192
           Y VSS++F+KF + +GDF++ +V D  G+LSL+EAS + ++GE ILDEAL FT  HL  M
Sbjct: 124 YNVSSDVFDKFKDCKGDFKKHLVNDVQGLLSLHEASYMSVQGEKILDEALEFTKTHL--M 183

Query: 193 AMDSKSPFSDEAKYALKWPMYKALPRFMARHYISLYDNNPLKNNVLLTFAKLDFNSLQKL 252
           A    SP  D+  +AL+WP+ + LPR  A  Y S+Y  +      LL  AKLDFN +QKL
Sbjct: 184 ATQLSSPLPDQVSHALRWPVRRGLPRKEAWQYFSIYQQDREHIEPLLKLAKLDFNIVQKL 243

Query: 253 YQKEVREFSRWLKNLKLMEQLCFARDRVVECYVWALGIYYEPKYSSARRILVKTISFISV 312
           + K++   +RW  +L    +L FARDRV+EC  WALG++YEP++  AR++L K ++ +SV
Sbjct: 244 HHKDMSIITRWWIDLDFTTKLSFARDRVIECSFWALGVFYEPQFVFARQVLSKAVAILSV 303

Query: 313 LDDLYDVHATLEELQLFTNAIERWDVNCIEKLPNYMKGLYEAILEVYREIEQDICKNNNI 372
           +DD+YDVH T+EEL+LFT  +ERWD++  ++LP+YMK  +EA+++ Y EIE +  K    
Sbjct: 304 MDDIYDVHGTIEELELFTEVVERWDISMKDQLPDYMKWYFEALIDFYAEIEAETTKGGR- 363

Query: 373 TFAFDYAKEAVNSLVMRAYFVEAKWFNEGYVPTMEEYMKVAITSAAYYLFASVSFLALGN 432
           +F   YAKEAV   V RAY  EA+WFN  YVPT+EEY+  A+ S+ Y +  ++SF  +G 
Sbjct: 364 SFCIHYAKEAVKKQV-RAYITEARWFNNDYVPTLEEYISNAVISSTYPILITLSFCGMGK 423

Query: 433 VASKEVFQWAQSDP-MLLKATGVIGRLLNDIASHKFEQERGHVASAVECYMKQYGVSEEK 492
            ASK+VF W  ++P  LL     + RL++DI SH+FEQERGHVASAVECYMKQ+ VS+++
Sbjct: 424 FASKDVFDWLFTEPNKLLYTASGLARLIDDIRSHEFEQERGHVASAVECYMKQHSVSKQE 483

Query: 493 ALNELENQVVEAWKDVIEDYTK-SAKFPNVILDCVLNVARLSDLFYKEGDGYTFADGETK 552
           A NEL + VV  WKD+ E+  K +   P  IL C+LN+ R+ D+ YK+ D YT +    K
Sbjct: 484 AYNELNSIVVNMWKDLNEELLKETGVLPKPILACILNIVRVMDVVYKDEDSYTNSRNSLK 543

Query: 553 RFITLILKDPVPI 563
             +   L +PVP+
Sbjct: 544 DILATFLVNPVPV 547

BLAST of Spg015772 vs. ExPASy Swiss-Prot
Match: B2KSJ6 (Alpha-farnesene synthase OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 492.3 bits (1266), Expect = 7.4e-138
Identity = 272/555 (49.01%), Postives = 362/555 (65.23%), Query Frame = 0

Query: 22  VPRRSANFQPSSWGDYFLYYHSNHPNQEDEDMVREQLRKLKEEVRSMLAAAEKCS--EKL 81
           V RRSA FQ S WGDYFL YHS  P ++   ++ +Q  +LKEE++  L +  K    EKL
Sbjct: 14  VIRRSAQFQASVWGDYFLSYHSLSP-EKGNKVMEKQTEELKEEIKRELNSTTKDEEPEKL 73

Query: 82  SLIDSIQRLGLSYHFEDEINEILEPMQKL---CNVDNDDDIYTVALRFRLLRQQGYFVSS 141
            LIDSIQRLG+ YHFE EIN+ILE +  +      + DD  Y + LRFRLLRQQGY +SS
Sbjct: 74  RLIDSIQRLGVCYHFEYEINKILEQLHHITITSKNNGDDHPYNMTLRFRLLRQQGYNISS 133

Query: 142 EIFNKFTNARGDFEESIVKDEGGILSLYEASQLRMKGENILDEALAFTTIHLQAMAMDSK 201
           + F +F   RG +E S  K+   +LSLYEASQLRM+GE  LDEA  F T  L+A+  D  
Sbjct: 134 KSFERF---RGKWESSYDKNVEELLSLYEASQLRMRGEEALDEAFRFATAQLEAIVQD-- 193

Query: 202 SPFSD-----EAKYALKWPMYKALPRFMARHYISLYDNNPLKNNVLLTFAKLDFNSLQKL 261
            P +D     E   ALKWPMYK LPR  A HYI LY   P +N  L  FAK+DF+ LQKL
Sbjct: 194 -PTTDPTVVGEVCQALKWPMYKNLPRLQASHYIGLYSEKPWRNESLPNFAKMDFSKLQKL 253

Query: 262 YQKEVREFSRWLKNLKLMEQLCFARDRVVECYVWALGIYYEPKYSSARRILVKTISFISV 321
           +QKE+   S+W  +    E+L FAR+R+VE Y +ALGI++EP+ S+AR I+ K I+  SV
Sbjct: 254 HQKEIAYISKWWDDYGFAEKLSFARNRIVEGYFFALGIFFEPQLSTARLIMTKIIAIGSV 313

Query: 322 LDDLYDVHATLEELQLFTNAIERWDVNCIEKLPNYMKGLYEAILEVYREIEQDICKNNNI 381
           LDD+YDV+ T EEL+L T A+ERWD +  +KLP YMK  YEA+L+V+ EIEQ++ +    
Sbjct: 314 LDDIYDVYGTFEELKLLTLALERWDKSETKKLPKYMKMYYEALLDVFEEIEQEMSQKETT 373

Query: 382 TFAFDYAKEAVNSLVMRAYFVEAKWFNEGYVPTMEEYMKVAITSAAYYLFASVSFLALGN 441
            +     KEA   L  R + VEAKW  EGY PT+EEY+ +A+ S  + L    + L +G+
Sbjct: 374 PYCIHQMKEATKEL-GRVFLVEAKWCKEGYTPTVEEYLDIALISFGHKLLMVTALLGMGS 433

Query: 442 -VASKEVFQWAQSDPMLLKATGVIGRLLNDIASHKFEQERGHVASAVECYMKQYGVSEEK 501
            +A++++ QW  S P +LKA+ +I RL+NDI SHKFEQERGHVASA+ECYM+Q  +SE  
Sbjct: 434 TIATQQIVQWITSMPNILKASAIICRLMNDIVSHKFEQERGHVASAIECYMEQNYMSEHD 493

Query: 502 ALNELENQVVEAWKDVIEDY---TKSAKFPNVILDCVLNVARLSDLFYKEGDGYTFADGE 561
            L  L  Q+ E WKD++E+Y       + P  +L  VLN+ RL ++ YK+GDGYT + G 
Sbjct: 494 VLIILGKQIDEFWKDMVENYCVVITEEEVPRGVLMRVLNLTRLFNVIYKDGDGYTQSHGS 553

Query: 562 TKRFITLILKDPVPI 563
           TK  I  +L D +P+
Sbjct: 554 TKTHIKSLLVDSLPL 560

BLAST of Spg015772 vs. ExPASy Swiss-Prot
Match: B2KSJ5 ((+)-gamma-cadinene synthase OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 3.7e-137
Identity = 264/555 (47.57%), Postives = 362/555 (65.23%), Query Frame = 0

Query: 22  VPRRSANFQPSSWGDYFLYYHSNHPNQEDEDMVREQLRKLKEEVRSMLAA-AEKCSEKLS 81
           V R  ANF P+ W ++FL +  +   + DE M +E+  KLKEE+R M+ A  E    KL+
Sbjct: 21  VKRSLANFHPNIWKEHFLSFTFDDALKIDEGM-KERTEKLKEEIRMMMIAYVENQLIKLN 80

Query: 82  LIDSIQRLGLSYHFEDEINEILEPMQKLCN--------VDNDDDIYTVALRFRLLRQQGY 141
           L+DSIQRLG+SYHFEDE++E LE +    N          N +D++  AL FRLLRQQGY
Sbjct: 81  LVDSIQRLGVSYHFEDEVDEFLEHIYVSYNNSLLLSNKNSNGEDLHITALLFRLLRQQGY 140

Query: 142 FVSSEIFNKFTNARGDFEESIVKDEGGILSLYEASQLRMKGENILDEALAFTTIHLQAMA 201
            +S +IF KF +  G F+ES+V+DE G+LSLYEAS +   GE +L+EAL FTT HLQ   
Sbjct: 141 RISCDIFLKFMDDNGKFKESLVEDERGLLSLYEASHMMGHGEALLEEALEFTTTHLQTYI 200

Query: 202 ---MDSKSPFSDEAKYALKWPMYKALPRFMARHYISLYDNNPLKNNVLLTFAKLDFNSLQ 261
               +    F+ E   ALK P+ K++PR  AR Y+ +Y  +P  N  LL F+KLDFN LQ
Sbjct: 201 HRYSNINPSFASEVSNALKLPIRKSVPRIKAREYLEIYQQHPSHNETLLEFSKLDFNILQ 260

Query: 262 KLYQKEVREFSRWLKNLKLMEQLCFARDRVVECYVWALGIYYEPKYSSARRILVKTISFI 321
           KL+QKE+ E  RW K+L +  +  FARDR+VECY W LG Y+EP+YS  R++L K I+  
Sbjct: 261 KLHQKELSEICRWWKDLDVPTKFPFARDRIVECYFWTLGAYFEPQYSVGRKMLTKVIAIA 320

Query: 322 SVLDDLYDVHATLEELQLFTNAIERWDVNCIEKLPNYMKGLYEAILEVYREIEQDICKNN 381
           S+LDD+YD + T EELQ+ T AI+RWD + +  LP YMK  Y A+LE+Y EI ++I K+ 
Sbjct: 321 SILDDIYDAYGTFEELQVLTPAIQRWDRSMVHTLPLYMKPFYVAMLELYEEIGKEIDKDQ 380

Query: 382 NITFAFDYAKEAVNSL--VMRAYFVEAKWFNEGYVPTMEEYMKVAITSAAYYLFASVSFL 441
           N      + + A+  +  +  +YF EAKW N+ Y P+ +EYM++A+ +  Y +  S+SFL
Sbjct: 381 NSL----HLQVAIGGIKRLSESYFEEAKWLNKEYKPSFKEYMELALKTTGYTMLISISFL 440

Query: 442 ALG-NVASKEVFQWAQSDPMLLKATGVIGRLLNDIASHKFEQERGHVASAVECYMKQYGV 501
            LG ++ + EV QW  + P ++KA+ +I RL++DIASHKFEQER HVASAVECYMKQY  
Sbjct: 441 GLGDHIVTNEVLQWLSNGPQIIKASTIICRLMDDIASHKFEQEREHVASAVECYMKQYDC 500

Query: 502 SEEKALNELENQVVEAWKDVIEDYTKSAKFPNVILDCVLNVARLSDLFYKEGDGYTFADG 561
           SEE+A  EL  +VV+AWKD  E + +    P  +L  VLN +R+ +L Y + DGYT A  
Sbjct: 501 SEEEACIELHKEVVDAWKDTNEAFYRPFNVPVPVLMRVLNFSRVINLLYLDEDGYTNAKS 560

BLAST of Spg015772 vs. ExPASy TrEMBL
Match: A0A6J1CQA8 ((-)-germacrene D synthase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111013331 PE=4 SV=1)

HSP 1 Score: 833.2 bits (2151), Expect = 6.6e-238
Identity = 419/558 (75.09%), Postives = 484/558 (86.74%), Query Frame = 0

Query: 6   LVSDSMAPCNASFNDSVPRRSANFQPSSWGDYFLYYHSNHPNQEDEDMVREQLRKLKEEV 65
           +VS+  AP N   ND V RRSANFQPS WGDYFL+   +H N + +DMV ++L+KLKEEV
Sbjct: 5   VVSNLTAPDNDPSNDGVLRRSANFQPSVWGDYFLF---SHINTDMDDMVGKKLQKLKEEV 64

Query: 66  RSMLAAAEKCSEKLSLIDSIQRLGLSYHFEDEINEILEPMQKLCNV-DNDDDIYTVALRF 125
           RSMLAAAEKCS+KLSLIDSIQRLGLS+HFE+EINEILE MQ   ++ D+D+D+  VALRF
Sbjct: 65  RSMLAAAEKCSKKLSLIDSIQRLGLSHHFEEEINEILEQMQNXRSLNDDDEDVDIVALRF 124

Query: 126 RLLRQQGYFVSSEIFNKFTNARGDFEESIVKDEGGILSLYEASQLRMKGENILDEALAFT 185
           RLLRQ+GY VS EIFN+F N  GDF+ES+VKDE GILSLYEAS LR+ GE+ILD+ALAFT
Sbjct: 125 RLLRQRGYSVSCEIFNRFINGNGDFKESMVKDECGILSLYEASHLRINGEDILDKALAFT 184

Query: 186 TIHLQAMAMDSKSPFSDEAKYALKWPMYKALPRFMARHYISLYDNNPLKNNVLLTFAKLD 245
           T HLQAMAMDS SPF++EAKYALKWP+YKALPRFM RHYISLYDN+PLK+NVLLTF+KLD
Sbjct: 185 TTHLQAMAMDSSSPFAEEAKYALKWPIYKALPRFMTRHYISLYDNDPLKSNVLLTFSKLD 244

Query: 246 FNSLQKLYQKEVREFSRWLKNLKLMEQLCFARDRVVECYVWALGIYYEPKYSSARRILVK 305
           FN+LQKLYQKE+ EFSRW K+LKLMEQ  FARDRVVECY W LGIYYEPKYS+ARRILVK
Sbjct: 245 FNALQKLYQKELVEFSRWWKDLKLMEQFSFARDRVVECYFWTLGIYYEPKYSNARRILVK 304

Query: 306 TISFISVLDDLYDVHATLEELQLFTNAIERWDVNCIEKLPNYMKGLYEAILEVYREIEQD 365
            I+FIS+LDD+ D +ATL+ELQLFT+AIERWD+N I+KLPNYMK + EAIL+VYRE+EQD
Sbjct: 305 VIAFISLLDDMXDAYATLDELQLFTDAIERWDINGIDKLPNYMKVIXEAILQVYREVEQD 364

Query: 366 ICKNNNITFAFDYAKEAVNSLVMRAYFVEAKWFNEGYVPTMEEYMKVAITSAAYYLFASV 425
           I K+NNI F  D AKE++     RAY VEAKWF++G+VP+MEEYM+VAI SAAYYLFASV
Sbjct: 365 IGKDNNILFGLDCAKESMKR-QCRAYLVEAKWFHKGHVPSMEEYMEVAIVSAAYYLFASV 424

Query: 426 SFLALGNVASKEVFQWAQSDPMLLKATGVIGRLLNDIASHKFEQERGHVASAVECYMKQY 485
           SFL +G+VASKE FQW  SDPMLL ATGVIGRL+NDIASHKFEQERGHVASAVECYMKQY
Sbjct: 425 SFLGMGDVASKEAFQWVMSDPMLLNATGVIGRLVNDIASHKFEQERGHVASAVECYMKQY 484

Query: 486 GVSEEKALNELENQVVEAWKDVIEDYTKSAKFPNVILDCVLNVARLSDLFYKEGDGYTFA 545
            VSEE+A+ ELE QV++AWKD+ EDY KS +FPN I+ CV NVARLSDLFYKEGDGYTF 
Sbjct: 485 EVSEEEAVGELEKQVIKAWKDITEDYMKSTRFPNAIVACVTNVARLSDLFYKEGDGYTFP 544

Query: 546 DGETKRFITLILKDPVPI 563
           DGET+RFIT ILKDPVP+
Sbjct: 545 DGETRRFITSILKDPVPL 558

BLAST of Spg015772 vs. ExPASy TrEMBL
Match: A0A6J1CPK3 ((-)-germacrene D synthase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111013401 PE=4 SV=1)

HSP 1 Score: 803.9 bits (2075), Expect = 4.3e-229
Identity = 409/554 (73.83%), Postives = 466/554 (84.12%), Query Frame = 0

Query: 11  MAPCNASFNDSV-PRRSANFQPSSWGDYFLYYHSNHPNQEDEDMVREQLRKLKEEVRSML 70
           MAP +   ND V  RRSANF+PS WGDYFL   S+  N   +DMVR+QL + +EEVR ML
Sbjct: 4   MAPNDGYSNDGVLRRRSANFEPSLWGDYFL---SSPVNTVLDDMVRKQLEEQREEVRRML 63

Query: 71  AAAEKCSEKLSLIDSIQRLGLSYHFEDEINEILEPMQK-LCNVDNDDDIYTVALRFRLLR 130
            AAEKCSEKLSLIDSIQRLG+SYHFE EINEILE MQ   CN+D+D+DIY VALRFRLLR
Sbjct: 64  EAAEKCSEKLSLIDSIQRLGISYHFEGEINEILEHMQNDSCNLDDDEDIYIVALRFRLLR 123

Query: 131 QQGYFVSSEIFNKFTNARGDFEESIVKDEGGILSLYEASQLRMKGENILDEALAFTTIHL 190
           QQGY VS EIFN+FTN  GDF+E+++KDE GILSLYEAS LRM GENILD+ALAFTT HL
Sbjct: 124 QQGYSVSCEIFNRFTNGDGDFKETMLKDECGILSLYEASHLRMNGENILDKALAFTTTHL 183

Query: 191 QAMAMDSKSPFSDEAKYALKWPMYKALPRFMARHYISLYDNNPLKNNVLLTFAKLDFNSL 250
           QAMAM S SPFS+EAKYALKWP+YKALPRF  RHYISLYD +PLK NVLL+FAKLDFN+L
Sbjct: 184 QAMAMSSSSPFSEEAKYALKWPIYKALPRFTTRHYISLYDKDPLKTNVLLSFAKLDFNAL 243

Query: 251 QKLYQKEVREFSRWLKNLKLMEQLCFARDRVVECYVWALGIYYEPKYSSARRILVKTISF 310
           QKLYQKE+ E SRWLK+ KLME L FAR+RVVECY WALG++YEPKYS AR ILVK I F
Sbjct: 244 QKLYQKELAEASRWLKDRKLMEYLSFARNRVVECYFWALGVFYEPKYSYARSILVKIIVF 303

Query: 311 ISVLDDLYDVHATLEELQLFTNAIERWDVNCIEKLPNYMKGLYEAILEVYREIEQDICKN 370
           IS LDD+YDV+A L+ELQLFT+AIERWD+N I+KLP YMK LY AILEVY E+EQDI K+
Sbjct: 304 ISFLDDMYDVYAXLDELQLFTDAIERWDINGIDKLPGYMKVLYGAILEVYGEVEQDIGKD 363

Query: 371 NNITFAFDYAKEAVNSLVMRAYFVEAKWFNEGYVPTMEEYMKVAITSAAYYLFASVSFLA 430
           NNI+F  D AKEA+     RAYF EAKWF EGYVPTMEEYM+VA  SA+Y+LFA+ SFL 
Sbjct: 364 NNISFGLDCAKEAMKR-QCRAYFTEAKWFYEGYVPTMEEYMEVASISASYHLFAAASFLG 423

Query: 431 LGNVASKEVFQWAQSDPMLLKATGVIGRLLNDIASHKFEQERGHVASAVECYMKQYGVSE 490
           +G+VAS EVFQWA+SDP+LL ATGVIGRL+NDI SHKFEQERGHVASAVECYMKQYGVSE
Sbjct: 424 MGDVASNEVFQWAKSDPILLSATGVIGRLMNDIVSHKFEQERGHVASAVECYMKQYGVSE 483

Query: 491 EKALNELENQVVEAWKDVIEDYTKSAKFPNVILDCVLNVARLSDLFYKEGDGYTFADGET 550
            +A++ELE QV++AWKD+IEDY K  +FPN IL CV+NV RLSD FYKE DGYTF +GET
Sbjct: 484 GEAIHELEKQVIKAWKDIIEDYMKCTRFPNAILSCVMNVDRLSDFFYKEEDGYTFPNGET 543

Query: 551 KRFITLILKDPVPI 563
            RFIT +LKDPVP+
Sbjct: 544 NRFITSMLKDPVPL 553

BLAST of Spg015772 vs. ExPASy TrEMBL
Match: A0A1S4DXZ8 ((-)-germacrene D synthase-like OS=Cucumis melo OX=3656 GN=LOC103491132 PE=4 SV=1)

HSP 1 Score: 792.0 bits (2044), Expect = 1.7e-225
Identity = 404/553 (73.06%), Postives = 467/553 (84.45%), Query Frame = 0

Query: 15  NASFNDSV-PRRSANFQPSSWGDYFLYYHSNHPNQEDEDMVREQLRKLKEEVRSMLAAAE 74
           N SF+D V  RR+A F PS WGDYFL  H N+   ED  + ++Q +++KEEVRSM AA E
Sbjct: 11  NGSFSDGVLRRRAAEFPPSPWGDYFLSSHFNNQLVEDNVVEKQQFQEVKEEVRSMFAAVE 70

Query: 75  KCSEKLSLIDSIQRLGLSYHFEDEINEI----LEPMQKLCNVDNDDDIYTVALRFRLLRQ 134
             SE+LSLIDSIQRLGLS+HF++EINE+    +E  +K  + D D+++Y +AL+FR+LRQ
Sbjct: 71  NSSERLSLIDSIQRLGLSHHFQEEINEVVLEHIEKFRKDNDNDADENLYIIALQFRMLRQ 130

Query: 135 QGYFVSSEIFNKFTNARGDFEESIVKDEGGILSLYEASQLRMKGENILDEALAFTTIHLQ 194
           QGY+VS EIFNKFTN RG+F+ESI  DE G+LSLYEAS LR KGENILDEALAFTT HL+
Sbjct: 131 QGYYVSCEIFNKFTNERGEFKESIANDEVGLLSLYEASHLRTKGENILDEALAFTTTHLK 190

Query: 195 AMAMDSKSPFSDEAKYALKWPMYKALPRFMARHYISLYDNNPLK-NNVLLTFAKLDFNSL 254
           AMAMDS SPF +EAKYALKWP+YKA+PRFMAR YISL   NPLK NNVLLTFAKLD+NSL
Sbjct: 191 AMAMDSNSPFFEEAKYALKWPIYKAVPRFMARQYISLCHKNPLKTNNVLLTFAKLDYNSL 250

Query: 255 QKLYQKEVREFSRWLKNLKLMEQLCFARDRVVECYVWALGIYYEPKYSSARRILVKTISF 314
           QKLYQKE+ EFSRW K  KL EQL FAR RVVE YVWALG+ YEPKY + RRIL K I+F
Sbjct: 251 QKLYQKELGEFSRWWKEHKLKEQLPFARVRVVEIYVWALGVQYEPKYCNTRRILAKIITF 310

Query: 315 ISVLDDLYDVHATLEELQLFTNAIERWDVNCIEKLPNYMKGLYEAILEVYREIEQDICKN 374
           ISVLDD+YDV+ATL+ELQLFTNAI+RWDV+CIEKLPNYMKGLYE ILEVY EIE+DICK+
Sbjct: 311 ISVLDDMYDVYATLDELQLFTNAIQRWDVSCIEKLPNYMKGLYETILEVYEEIEKDICKD 370

Query: 375 NNITFAFDYAKEAVNSLVMRAYFVEAKWFNEGYVPTMEEYMKVAITSAAYYLFASVSFLA 434
           NNI FAFDYAKEA+ SL  +AYF+EAKWFNEGY+PT+EEYMKVA  SA+Y +FAS+SFL 
Sbjct: 371 NNIPFAFDYAKEALKSL-CKAYFMEAKWFNEGYIPTVEEYMKVARISASYNVFASISFLT 430

Query: 435 LGNVASKEVFQWAQSDPMLLKATGVIGRLLNDIASHKFEQERGHVASAVECYMKQYGVSE 494
           LGNVASKE ++WAQ+DP+LLKA GVIGRLLND+ SHKFEQERGHVASAVECY+K+Y VSE
Sbjct: 431 LGNVASKEFYEWAQTDPILLKAAGVIGRLLNDMVSHKFEQERGHVASAVECYVKEYEVSE 490

Query: 495 EKALNELENQVVEAWKDVIEDYTKSA-KFPNVILDCVLNVARLSDLFYKEGDGYTFADGE 554
           E+AL ELE +V  AWKDV EDYTKS+ KFPNVIL+CVLNVARLSD FYKE DGYTFADGE
Sbjct: 491 EEALIELEKEVGTAWKDVTEDYTKSSLKFPNVILECVLNVARLSDFFYKEVDGYTFADGE 550

Query: 555 TKRFITLILKDPV 561
           TK FI L+L  PV
Sbjct: 551 TKHFIDLMLTHPV 562

BLAST of Spg015772 vs. ExPASy TrEMBL
Match: A0A6J1CQI9 ((-)-germacrene D synthase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111013402 PE=4 SV=1)

HSP 1 Score: 790.0 bits (2039), Expect = 6.4e-225
Identity = 400/561 (71.30%), Postives = 470/561 (83.78%), Query Frame = 0

Query: 3   SQTLVSDSMAPCNASFNDSVPRRSANFQPSSWGDYFLYYHSNHPNQEDEDMVREQLRKLK 62
           S  +VSD +AP N S NDSV RRSANF+PS WGDYFL Y     N   +DMVR+QL + +
Sbjct: 2   SSEVVSDLIAPNNGSSNDSVLRRSANFEPSLWGDYFLSYPI---NTVLDDMVRKQLEEQR 61

Query: 63  EEVRSMLAAAEKCSEKLSLIDSIQRLGLSYHFEDEINEILEPMQK-LCNVDNDDDIYTVA 122
           EEVR ML AAEKCSEKLSLIDSIQRLGLSYHFE EINEILE MQ   CN+D+D+DIY VA
Sbjct: 62  EEVRRMLEAAEKCSEKLSLIDSIQRLGLSYHFEGEINEILEHMQNDSCNLDDDEDIYIVA 121

Query: 123 LRFRLLRQQGYFVSSEIFNKFTNARGDFEESIVKDEGGILSLYEASQLRMKGENILDEAL 182
           LRFRLLRQQGY VS EIFN+FTN  GDF+ES+VKD+ GILSLY+AS L M GENILD+AL
Sbjct: 122 LRFRLLRQQGYSVSCEIFNRFTNENGDFKESMVKDQRGILSLYQASHLGMNGENILDKAL 181

Query: 183 AFTTIHLQAMAMDSKSPFSDEAKYALKWPMYKALPRFMARHYISLYDNNPLKNNVLLTFA 242
           AFTT HLQA+AMD+ SPF++EAKY+LKWP+YKA+PRF AR++ISLYD +PLKNNVLL+FA
Sbjct: 182 AFTTTHLQAIAMDASSPFAEEAKYSLKWPIYKAVPRFTARNHISLYDKDPLKNNVLLSFA 241

Query: 243 KLDFNSLQKLYQKEVREFSRWLKNLKLMEQLCFARDRVVECYVWALGIYYEPKYSSARRI 302
           KLDFN+LQKLY+KE+ E SRWLK+ KLME L FAR+RVVECY WALG++YEPKYS AR I
Sbjct: 242 KLDFNALQKLYKKELAEVSRWLKDQKLMENLSFARNRVVECYFWALGVFYEPKYSYARSI 301

Query: 303 LVKTISFISVLDDLYDVHATLEELQLFTNAIERWDVNCIEKLPNYMKGLYEAILEVYREI 362
           LVK I FIS LDD+YDV+ATL+ELQLFT+AIERWD+N I+KLP+YMK LY+AIL+ YR++
Sbjct: 302 LVKNIIFISFLDDMYDVYATLDELQLFTDAIERWDINAIDKLPHYMKFLYQAILDAYRDV 361

Query: 363 EQDICKNNNI-TFAFDYAKEAVNSLVMRAYFVEAKWFNEGYVPTMEEYMKVAITSAAYYL 422
           EQDI ++NNI +F  D AK A+     R Y  EAKWF EGYVPTMEEYM++A  SA+Y+L
Sbjct: 362 EQDIDEDNNILSFGLDCAKAAMKR-QCRVYLAEAKWFYEGYVPTMEEYMEIATISASYHL 421

Query: 423 FASVSFLALGNVASKEVFQWAQSDPMLLKATGVIGRLLNDIASHKFEQERGHVASAVECY 482
           FA+ SFL +G+V SKEV +WA+SDP++L A+G IGRL+NDI SH+FEQERG VASAVECY
Sbjct: 422 FAASSFLGMGDVVSKEVLEWAKSDPIMLSASGDIGRLMNDIVSHEFEQERGDVASAVECY 481

Query: 483 MKQYGVSEEKALNELENQVVEAWKDVIEDYTKSAKFPNVILDCVLNVARLSDLFYKEGDG 542
           MKQYGVSEE+A+ ELE QV++AWKDV EDY KS KFPN IL CV+NV RLSD FYKE DG
Sbjct: 482 MKQYGVSEEEAVVELEKQVIKAWKDVTEDYMKSNKFPNAILSCVINVNRLSDFFYKEEDG 541

Query: 543 YTFADGETKRFITLILKDPVP 562
           YTF +GE KRFIT +LKDPVP
Sbjct: 542 YTFPNGEIKRFITSMLKDPVP 558

BLAST of Spg015772 vs. ExPASy TrEMBL
Match: A0A6J1CQI4 ((-)-germacrene D synthase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111013399 PE=4 SV=1)

HSP 1 Score: 774.2 bits (1998), Expect = 3.6e-220
Identity = 393/559 (70.30%), Postives = 459/559 (82.11%), Query Frame = 0

Query: 6   LVSDSMAPCNASFNDSVPRRSANFQPSSWGDYFLYYHSNHPNQEDEDMVREQLRKLKEEV 65
           +VSD + P N S NDSV RRS NF+ S WGDYFL   S+  N   +D+VR+QL + KE V
Sbjct: 5   VVSDLIVPNNGSSNDSVLRRSGNFEQSLWGDYFL---SSPINTVLDDVVRKQLEEQKEGV 64

Query: 66  RSMLAAAEKCSEKLSLIDSIQRLGLSYHFEDEINEILEPMQK-LCNVDNDDDIYTVALRF 125
           R M+ AA+KCSEKLSLIDSIQRLGLSYHFE EINEILE MQ   CN+D+D+DIY VALRF
Sbjct: 65  RRMVEAADKCSEKLSLIDSIQRLGLSYHFEGEINEILEHMQNDSCNLDDDEDIYIVALRF 124

Query: 126 RLLRQQGYFVSSEIFNKFTNARGDFEESIVKDEGGILSLYEASQLRMKGENILDEALAFT 185
           RLLRQQGY VS EIFN+FT   GDF+ES+VKDE GILSLY+AS L M GENILD+ALAFT
Sbjct: 125 RLLRQQGYSVSCEIFNRFTTENGDFKESMVKDECGILSLYQASHLGMNGENILDKALAFT 184

Query: 186 TIHLQAMAMDSKSPFSDEAKYALKWPMYKALPRFMARHYISLYDNNPLKNNVLLTFAKLD 245
           T HLQAMAMDS SPF++EAKYALKWP+YKA+PR   RHYISLYD +PLKN+VLL+FAKLD
Sbjct: 185 TTHLQAMAMDSSSPFAEEAKYALKWPIYKAMPRXTTRHYISLYDRDPLKNSVLLSFAKLD 244

Query: 246 FNSLQKLYQKEVREFSRWLKNLKLMEQLCFARDRVVECYVWALGIYYEPKYSSARRILVK 305
           FN+LQKLYQKE+ E SRW+K+ KLME L FAR+RVV CY WALG++YEPKYS AR IL K
Sbjct: 245 FNALQKLYQKELAEVSRWMKDQKLMEHLSFARNRVVXCYFWALGVFYEPKYSYARIILAK 304

Query: 306 TISFISVLDDLYDVHATLEELQLFTNAIERWDVNCIEKLPNYMKGLYEAILEVYREIEQD 365
            I FIS LDD+YDV+ATL ELQLFT+A++RWD+N I KLPNYMK LYEAILEVY E+EQD
Sbjct: 305 IIIFISFLDDMYDVYATLAELQLFTDAMDRWDINVIHKLPNYMKFLYEAILEVYGEVEQD 364

Query: 366 ICKNNNI-TFAFDYAKEAVNSLVMRAYFVEAKWFNEGYVPTMEEYMKVAITSAAYYLFAS 425
           I K+NNI +F  D AKEA+     RAYF EAKWF E YVPTMEEYM+VA  SA+Y+LF +
Sbjct: 365 IGKDNNISSFGLDCAKEAMKR-QCRAYFAEAKWFYEXYVPTMEEYMEVARISASYHLFVA 424

Query: 426 VSFLALGNVASKEVFQWAQSDPMLLKATGVIGRLLNDIASHKFEQERGHVASAVECYMKQ 485
            SFL +G+V SKE  QWA+SDP++L A+G IGRL+NDI SHKFEQE G VASAVECYMKQ
Sbjct: 425 ASFLGMGDVVSKEALQWAKSDPIILSASGDIGRLMNDIVSHKFEQEXGDVASAVECYMKQ 484

Query: 486 YGVSEEKALNELENQVVEAWKDVIEDYTKSAKFPNVILDCVLNVARLSDLFYKEGDGYTF 545
           YG+SEE+ + ELE QV++AWKD+ EDY KS KFPN IL CV+ V+RLSD FYKE DGYTF
Sbjct: 485 YGISEEETIVELEKQVIKAWKDITEDYMKSTKFPNAILSCVMXVSRLSDFFYKEEDGYTF 544

Query: 546 ADGETKRFITLILKDPVPI 563
           ++GE +RFIT +LKDPVP+
Sbjct: 545 SNGEIRRFITSMLKDPVPL 559

BLAST of Spg015772 vs. TAIR 10
Match: AT5G23960.2 (terpene synthase 21 )

HSP 1 Score: 350.9 bits (899), Expect = 1.9e-96
Identity = 192/547 (35.10%), Postives = 317/547 (57.95%), Query Frame = 0

Query: 22  VPRRSANFQPSSWGDYFLYYHSNHPNQEDEDMVREQLRKLKEEVR-SMLAAAEKCSEKLS 81
           V R  A+F  + W D    +  +    E     +E+   LKE V+ + +++     E + 
Sbjct: 5   VNRPLADFPANIWEDPLTSFSKSDLGTE---TFKEKHSTLKEAVKEAFMSSKANPIENIK 64

Query: 82  LIDSIQRLGLSYHFEDEINEILEPMQKLCNV-----DNDDDIYTVALRFRLLRQQGYFVS 141
            ID++ RLG+SYHFE +I E L+      +          D+YTV + F++ RQ G+ +S
Sbjct: 65  FIDALCRLGVSYHFEKDIVEQLDKSFDCLDFPQMVRQEGCDLYTVGIIFQVFRQFGFKLS 124

Query: 142 SEIFNKFTNARGDFEESIVKDEGGILSLYEASQLRMKGENILDEALAFTTIHLQAMAMDS 201
           +++F KF +  G F+  +V D  G+LSLYEA+Q    GE+I+DEALAF+  HL+ ++  S
Sbjct: 125 ADVFEKFKDENGKFKGHLVTDAYGMLSLYEAAQWGTHGEDIIDEALAFSRSHLEEISSRS 184

Query: 202 KSPFSDEAKYALKWPMYKALPRFMARHYISLYDNNPLKNNVLLTFAKLDFNSLQKLYQKE 261
               +   K ALK P +K + R   R YIS Y+     +  LL FAK+DFN LQ L+++E
Sbjct: 185 SPHLAIRIKNALKHPYHKGISRIETRQYISYYEEEESCDPTLLEFAKIDFNLLQILHREE 244

Query: 262 VREFSRWLKNLKLMEQLCFARDRVVECYVWALGIYYEPKYSSARRILVKTISFISVLDDL 321
           +   +RW   ++   ++ + R R+ E Y+W+LG Y+EP+YS AR I    +   + LDD+
Sbjct: 245 LACVTRWHHEMEFKSKVTYTRHRITEAYLWSLGTYFEPQYSQARVITTMALILFTALDDM 304

Query: 322 YDVHATLEELQLFTNAIERWDVNCIEKLPNYMKGLYEAILEVYREIEQDICKNNNITFAF 381
           YD + T+EEL+LFT+A++ + +  I  +P+ MK +Y   +E Y ++++++ K       F
Sbjct: 305 YDAYGTMEELELFTDAMDEYSICNIIPIPDSMKFIYNVTVEFYDKLDEELEKEGRSGCGF 364

Query: 382 DYAKEAVNSLVMRAYFVEAKWFNEGYVPTMEEYMKVAITSAAYYLFASVSFLALGNVASK 441
              K    +     Y  EAKW  + Y+ T +EY + AI S+ YY   +++F+ + +VA  
Sbjct: 365 HLKKSLQKT--ANGYMQEAKWLKKDYIATFDEYKENAILSSGYYALIAMTFVRMTDVAKL 424

Query: 442 EVFQWAQSDPMLLKATGVIGRLLNDIASHKFEQERGHVASAVECYMKQYGVSEEKALNEL 501
           + F+W  S P +  A+ +I R  +DI+S++FE +R HVA+ ++CYM+Q+GVS+E+A+  +
Sbjct: 425 DAFEWLSSHPKIRVASEIISRFTDDISSYEFEHKREHVATGIDCYMQQFGVSKERAVEVM 484

Query: 502 ENQVVEAWKDVIEDYTKSAKFPNVILDCVLNVARLSDLFYKEGDGYTFADGETKRFITLI 561
            N V +AWKD+ ++  +   FP  +L  VLN++R+ D+FY+  D YT      K  I  +
Sbjct: 485 GNIVSDAWKDLNQELMRPHVFPFPLLMRVLNLSRVIDVFYRYQDAYT-NPKLLKEHIVSL 544

Query: 562 LKDPVPI 563
           L + +PI
Sbjct: 545 LIETIPI 545

BLAST of Spg015772 vs. TAIR 10
Match: AT1G70080.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 349.4 bits (895), Expect = 5.5e-96
Identity = 202/563 (35.88%), Postives = 328/563 (58.26%), Query Frame = 0

Query: 12  APCNASFNDSVPRRSANFQPSSWGDYFLYYHSNHPNQEDEDMVREQLRKLKEEVRSMLAA 71
           A  N + ++S  R      PS WG +FL   S   NQ + D +  Q+  LK  V +ML  
Sbjct: 54  ATTNPTDDNSTTRSFTPHPPSLWGHHFL---SASVNQTEMDDLWRQIEALKPIVNAMLLP 113

Query: 72  AEKCSEK--LSLIDSIQRLGLSYHFEDEINEIL----EPMQKLCNVDNDDDIYTVALRFR 131
                 K     I ++  LG+SYHFE++I E L    E ++ +     +DD+YTV++ FR
Sbjct: 114 CNGADAKKITCFIHTLVSLGVSYHFEEKIVEFLKDAFENIEDMIIDCKEDDLYTVSIIFR 173

Query: 132 LLRQQGYFVSSEIFNKFTNARGDFEESIVKDEGGILSLYEASQLRMKGENILDEALAFTT 191
           + R  G++++ +IFN+F    G+F++ +  D  G+LS YEAS      E+IL+EA++FT 
Sbjct: 174 VFRLYGHYITPDIFNRFKGDDGNFKKCLNDDVRGMLSFYEASHFGTTTEDILEEAMSFTQ 233

Query: 192 IHLQAMAMDSKSP----FSDEAKYALKWPMYKALPRFMARHYISLYDNNPLKNNVLLTFA 251
            HL+   +  K+      +   + AL  P    L   +AR YI  Y+     N +LL  A
Sbjct: 234 KHLELFLVGEKAKHYPHITKLIQAALYIPQNFNLEILVAREYIDFYELETDHNEMLLKLA 293

Query: 252 KLDFNSLQKLYQKEVREFSRWLKNLKLMEQL-CFARDRVVECYVWALGIYYEPKYSSARR 311
           KL+F  LQ  Y ++++  + W K L L+ ++  + R+R+ E Y WA GIYYEP+YS+AR 
Sbjct: 294 KLNFRFLQLQYIQDLKTLTTWWKELDLVSKIPVYFRERLAEPYFWATGIYYEPQYSAARI 353

Query: 312 ILVKTISFISVLDDLYDVHATLEELQLFTNAIERWDVNCIEKLPNYMKGLYEAILEVYRE 371
           +L K+I  + ++D+ +DV+ T++E++    AIERWD + ++ LP+Y+K ++    ++++E
Sbjct: 354 MLAKSIILVDIVDNTFDVYGTIDEVKSLVQAIERWDSDAVDVLPDYLKVVFRTTFDLFKE 413

Query: 372 IEQDICKNNNITFAFDYAKEAVNSLVMRAYFVEAKWFNEGYVPTMEEYMKVAITSAAYYL 431
           +E+ +      +F   YA E +  ++M+ Y  EA+W N G++P+ EEY++V + S A  +
Sbjct: 414 LEEYVSSEAR-SFTMQYAYEQLR-ILMKGYLQEAEWSNRGHLPSHEEYIEVGVASTAGEV 473

Query: 432 FASVSFLALGNVASKEVFQWAQSDPMLLKATGVIGRLLNDIASHKFEQERGHVASAVECY 491
             +++F+ +G+ A   V++W +S P L  A  V  RL +DIA++K E +RG V + + CY
Sbjct: 474 LLAMTFIPMGDAAGVGVYEWLRSRPKLTHALFVKSRLRDDIATYKEEMKRGDVCNGINCY 533

Query: 492 MKQYGVSEEKALNELENQVVEAWKDVIEDYTKSAKF-PNVILDCVLNVARLSDLFYKEGD 551
            KQ+ VSEE+A  E E +     K + E++ K+AKF P  IL  VLN  RL+D+ YK GD
Sbjct: 534 TKQHKVSEEEACIEFEKKTNHMSKVMNEEFLKAAKFIPLHILRPVLNYGRLADVCYKYGD 593

Query: 552 GYTFADGETKRFITLILKDPVPI 563
           GYTFA  + K +IT +  D + +
Sbjct: 594 GYTFAGEKIKDYITSLYVDLITL 611

BLAST of Spg015772 vs. TAIR 10
Match: AT5G23960.1 (terpene synthase 21 )

HSP 1 Score: 346.7 bits (888), Expect = 3.6e-95
Identity = 192/549 (34.97%), Postives = 317/549 (57.74%), Query Frame = 0

Query: 22  VPRRSANFQPSSWGDYFLYYHSNHPNQEDEDMVREQLRKLKEEVR-SMLAAAEKCSEKLS 81
           V R  A+F  + W D    +  +    E     +E+   LKE V+ + +++     E + 
Sbjct: 5   VNRPLADFPANIWEDPLTSFSKSDLGTE---TFKEKHSTLKEAVKEAFMSSKANPIENIK 64

Query: 82  LIDSIQRLGLSYHFEDEINEILEPMQKLCNV-----DNDDDIYTVALRFRLLRQQGYFVS 141
            ID++ RLG+SYHFE +I E L+      +          D+YTV + F++ RQ G+ +S
Sbjct: 65  FIDALCRLGVSYHFEKDIVEQLDKSFDCLDFPQMVRQEGCDLYTVGIIFQVFRQFGFKLS 124

Query: 142 SEIFNKFTNARGDFEESIVKDEGGILSLYEASQLRMKGENILDEALAFTTIHLQAMAMDS 201
           +++F KF +  G F+  +V D  G+LSLYEA+Q    GE+I+DEALAF+  HL+ ++  S
Sbjct: 125 ADVFEKFKDENGKFKGHLVTDAYGMLSLYEAAQWGTHGEDIIDEALAFSRSHLEEISSRS 184

Query: 202 KSPFSDEAKYALKWPMYKALPRFMARHYISLYDNNPLKNNVLLTFAKLDFNSLQKLYQKE 261
               +   K ALK P +K + R   R YIS Y+     +  LL FAK+DFN LQ L+++E
Sbjct: 185 SPHLAIRIKNALKHPYHKGISRIETRQYISYYEEEESCDPTLLEFAKIDFNLLQILHREE 244

Query: 262 VREFSRWLKNLKLMEQLCFARDRVVECYVWALGIYYEPKYSSARRILVKTISFISVLDDL 321
           +   +RW   ++   ++ + R R+ E Y+W+LG Y+EP+YS AR I    +   + LDD+
Sbjct: 245 LACVTRWHHEMEFKSKVTYTRHRITEAYLWSLGTYFEPQYSQARVITTMALILFTALDDM 304

Query: 322 YDVHATLEELQLFTNAIERWDVNCIEK--LPNYMKGLYEAILEVYREIEQDICKNNNITF 381
           YD + T+EEL+LFT+A++ W     ++  +P+ MK +Y   +E Y ++++++ K      
Sbjct: 305 YDAYGTMEELELFTDAMDEWLPVVPDEIPIPDSMKFIYNVTVEFYDKLDEELEKEGRSGC 364

Query: 382 AFDYAKEAVNSLVMRAYFVEAKWFNEGYVPTMEEYMKVAITSAAYYLFASVSFLALGNVA 441
            F   K    +     Y  EAKW  + Y+ T +EY + AI S+ YY   +++F+ + +VA
Sbjct: 365 GFHLKKSLQKT--ANGYMQEAKWLKKDYIATFDEYKENAILSSGYYALIAMTFVRMTDVA 424

Query: 442 SKEVFQWAQSDPMLLKATGVIGRLLNDIASHKFEQERGHVASAVECYMKQYGVSEEKALN 501
             + F+W  S P +  A+ +I R  +DI+S++FE +R HVA+ ++CYM+Q+GVS+E+A+ 
Sbjct: 425 KLDAFEWLSSHPKIRVASEIISRFTDDISSYEFEHKREHVATGIDCYMQQFGVSKERAVE 484

Query: 502 ELENQVVEAWKDVIEDYTKSAKFPNVILDCVLNVARLSDLFYKEGDGYTFADGETKRFIT 561
            + N V +AWKD+ ++  +   FP  +L  VLN++R+ D+FY+  D YT      K  I 
Sbjct: 485 VMGNIVSDAWKDLNQELMRPHVFPFPLLMRVLNLSRVIDVFYRYQDAYT-NPKLLKEHIV 544

Query: 562 LILKDPVPI 563
            +L + +PI
Sbjct: 545 SLLIETIPI 547

BLAST of Spg015772 vs. TAIR 10
Match: AT3G14540.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 345.9 bits (886), Expect = 6.1e-95
Identity = 200/547 (36.56%), Postives = 317/547 (57.95%), Query Frame = 0

Query: 24  RRSANFQPSSWGDYFLYYHSNHPNQEDEDMVREQLRKLKEEVRSMLAAAEKC-SEKLSLI 83
           R S  F PS WGD+FL    +H   E  ++ RE +  +K  V+ ML +++    EK+ LI
Sbjct: 60  RPSTYFSPSLWGDHFLSVSLDH--AEFVELERE-IETMKPLVKDMLMSSQSSDKEKIRLI 119

Query: 84  DSIQRLGLSYHFEDEINEILE-PMQKLCN-VDNDDDIYTVALRFRLLRQQGYFVSSEIFN 143
             +  LG SYHF+ EI +IL+    KL   +  +DD+ T+++ F + R  G+ +S + F+
Sbjct: 120 HLLVSLGSSYHFDKEIQDILKHSFTKLDGIIVEEDDLETISIMFEVFRLYGHKMSCDAFD 179

Query: 144 KFTNARGDFEESIVKDEGGILSLYEASQLRMKGENILDEALAFTTIHLQAMA---MDSKS 203
           +F    G F+ES+ KD  G+L L+E + L    E+I+DEAL FT  HL+++    + S S
Sbjct: 180 RFRGGDGRFKESLAKDVRGMLQLFEVAHLGTLSEDIMDEALRFTRNHLESLTSGNVSSAS 239

Query: 204 P-FSDEAKYALKWPMYKALPRFMARHYISLYDNNPLKNNVLLTFAKLDFNSLQKLYQKEV 263
           P      + +L  P Y  +   +AR YIS Y+     N +LL FAKL+FN  Q  Y +E+
Sbjct: 240 PHILKHIQNSLYIPRYCNIEVLVAREYISYYEQEEGYNEILLKFAKLNFNFCQCHYIQEI 299

Query: 264 REFSRWLKNLKLMEQLCFARDRVVECYVWALGIYYEPKYSSARRILVKTISFISVLDDLY 323
           +  ++W K+L L  +L + RDR VE ++  LG Y+EP+YS  R I+ KTI  I V DD Y
Sbjct: 300 KTLTKWWKDLDLASKLPYIRDRSVESHLGGLGPYFEPQYSLGRIIVAKTIMIIVVADDTY 359

Query: 324 DVHATLEELQLFTNAIERWDVNCIEKLPNYMKGLYEAILEVYREIEQDICKNNNITFAFD 383
           D HAT+ E  + T   +R ++   +KL  Y++ + E++ EV  EIEQ++      +++  
Sbjct: 360 DAHATIPEATVLTEYFQRLNIGADDKLSGYLRIVLESVFEVMGEIEQEMSPKGR-SYSVK 419

Query: 384 YAKEAVNSLVMRAYFVEAKWFNEGYVPTMEEYMKVAITSAAYYLFASVSFLALGNVASKE 443
              E    ++ +AY    +W  +G+VPT +EYMKV + +A    +A   F+ + ++  KE
Sbjct: 420 QVLERF-KIIAKAYKQLTEWARKGHVPTFDEYMKVGLVTAGMGDYAGYCFIGMEDINEKE 479

Query: 444 VFQWAQSDPMLLKATGVIGRLLNDIASHKFEQERGHVASAVECYMKQYGVSEEKALNELE 503
            F+W  S+P+L+ A  V+ R+ ND+ +++ E  RG VA+ + CYMKQYGV++E+A  EL 
Sbjct: 480 AFEWLNSNPLLIDALNVLFRIANDVGTYETEINRGEVANGLNCYMKQYGVTKEEASRELR 539

Query: 504 NQVVEAWKDVIEDYTKS-AKFPNVILDCVLNVARLSDLFYKEGDGYTFADGETKRFITLI 563
              +   K V+E++  S  + P  +L   LN ARL D+ Y EGDGY+   G+ + F+T +
Sbjct: 540 KMYIYNKKVVVEEFMNSHDRVPRQVLLRCLNFARLFDVIYTEGDGYSEPKGKIEHFMTSL 599

BLAST of Spg015772 vs. TAIR 10
Match: AT3G29410.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 338.2 bits (866), Expect = 1.3e-92
Identity = 192/542 (35.42%), Postives = 309/542 (57.01%), Query Frame = 0

Query: 29  FQPSSWGDYFLYYHSNHPNQEDEDMVREQLRKLKEEVRSMLAAAEKC-SEKLSLIDSIQR 88
           F PS WGD+FL    +  + E E + +E     K +VR ML +      E++ LI  +  
Sbjct: 69  FSPSYWGDHFLSVSID--DSEFEALEKEIETVFKPKVRDMLMSPHSSDKERIRLIHLLIS 128

Query: 89  LGLSYHFEDEINEILEPM--QKLCNVDNDDDIYTVALRFRLLRQQGYFVSSEIFNKFTNA 148
           LG++Y++E+EI EIL     +  C + ++DD+ T+A+ F + R  G+ +  ++F +F + 
Sbjct: 129 LGIAYYYENEIEEILHKAYGKLACLISDEDDLETIAIMFEVFRLYGHKMPCDVFERFKSE 188

Query: 149 RGDFEESIVKDEGGILSLYEASQLRMKGENILDEALAFTTIHLQAMA-MDSKSPFSDEAK 208
            G F+ES+V D  G+L LYEA+ L    E+I+DEAL+F   HL+ +A  ++ S      +
Sbjct: 189 DGKFKESLVGDVRGLLQLYEAAHLGAPSEDIMDEALSFARYHLEPLAGTETSSNLFKHVE 248

Query: 209 YALKWPMYKALPRFMARHYISLYDNNPLKNNVLLTFAKLDFNSLQKLYQKEVREFSRWLK 268
             L    Y ++   +AR YIS YD    ++  LL F+KL+FN  Q  Y KE++  +RW K
Sbjct: 249 NVLYRARYHSIEILVARQYISFYDQEEDQDETLLRFSKLNFNFCQMHYVKELKIVTRWWK 308

Query: 269 NLKLMEQLCFA-RDRVVECYVWALGIYYEPKYSSARRILVKTISFISVLDDLYDVHATLE 328
            L +  +L ++ R+R VE Y+  LG+ +EP+YS AR  L K    ++V+DD  D +ATL 
Sbjct: 309 ELGIASKLPYSIRERNVETYLGGLGVLFEPRYSLARIFLAKLTLIMTVVDDTCDAYATLP 368

Query: 329 ELQLFTNAIERWDVNCIEKLPNYMKGLYEAILEVYREIEQDIC---KNNNITFAFDYAKE 388
           E+Q   +A  RWD+  +E+LP YM+ +Y+++ E   +I++++    K+  +    D  K 
Sbjct: 369 EVQSLHDAFHRWDLRAMEELPRYMRIIYQSVFETVEDIDREMIARGKHGRLQLTIDEIKS 428

Query: 389 AVNSLVMRAYFVEAKWFNEGYVPTMEEYMKVAITSAAYYLFASVSFLALGNVASKEVFQW 448
                +M  Y   AKW     VP+ E+YM++   S+A   FAS  F+A+ +   K++ +W
Sbjct: 429 -----LMIWYLGIAKWARSDQVPSFEDYMEIGTPSSALDDFASYGFIAMDDCDQKQLKEW 488

Query: 449 AQSDPMLLKATGVIGRLLNDIASHKFEQERGHVASAVECYMKQYGVSEEKALNELENQVV 508
             S P +  A   + R+ NDI + + E  RG VA+ V CYMKQ+GV++E A+ EL     
Sbjct: 489 FYSKPKIFHALNALFRIRNDIVTFEQEMSRGEVANGVNCYMKQHGVTKEAAVEELRKMER 548

Query: 509 EAWKDVIEDYTKSAKFPNVILDCVLNVARLSDLFYKEGDGYTFADGETKRFITLILKDPV 563
           E++K +IE++  S   P  IL   +N+AR+ DLFYKE DG+   D +  + I  +   P+
Sbjct: 549 ESYKIMIEEFMTSKAMPRQILVRPVNIARVMDLFYKEADGFGHPDQKLLQLIASLFLHPI 603

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038877743.13.8e-24075.22LOW QUALITY PROTEIN: (-)-germacrene D synthase-like [Benincasa hispida][more]
XP_022143456.11.4e-23775.09(-)-germacrene D synthase-like isoform X1 [Momordica charantia][more]
KGN55986.24.5e-23372.08hypothetical protein Csa_011696 [Cucumis sativus][more]
XP_004151461.38.5e-23273.04(-)-germacrene D synthase [Cucumis sativus][more]
XP_038903578.15.5e-23181.45LOW QUALITY PROTEIN: (-)-germacrene D synthase-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q6Q3H32.7e-15651.18(-)-germacrene D synthase OS=Vitis vinifera OX=29760 GN=VIT_19s0014g04930 PE=1 S... [more]
B9SCB66.7e-14747.32Probable terpene synthase 2 OS=Ricinus communis OX=3988 GN=TPS2 PE=3 SV=1[more]
B9SBV62.2e-14548.10Probable terpene synthase 3 OS=Ricinus communis OX=3988 GN=TPS3 PE=3 SV=1[more]
B2KSJ67.4e-13849.01Alpha-farnesene synthase OS=Cucumis melo OX=3656 PE=1 SV=1[more]
B2KSJ53.7e-13747.57(+)-gamma-cadinene synthase OS=Cucumis melo OX=3656 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CQA86.6e-23875.09(-)-germacrene D synthase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A6J1CPK34.3e-22973.83(-)-germacrene D synthase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A1S4DXZ81.7e-22573.06(-)-germacrene D synthase-like OS=Cucumis melo OX=3656 GN=LOC103491132 PE=4 SV=1[more]
A0A6J1CQI96.4e-22571.30(-)-germacrene D synthase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A6J1CQI43.6e-22070.30(-)-germacrene D synthase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT5G23960.21.9e-9635.10terpene synthase 21 [more]
AT1G70080.15.5e-9635.88Terpenoid cyclases/Protein prenyltransferases superfamily protein [more]
AT5G23960.13.6e-9534.97terpene synthase 21 [more]
AT3G14540.16.1e-9536.56Terpenoid cyclases/Protein prenyltransferases superfamily protein [more]
AT3G29410.11.3e-9235.42Terpenoid cyclases/Protein prenyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 51..78
NoneNo IPR availableSFLDSFLDS00005Isoprenoid_Synthase_Type_Icoord: 233..559
e-value: 0.0
score: 361.0
NoneNo IPR availablePANTHERPTHR31225OS04G0344100 PROTEIN-RELATEDcoord: 22..546
NoneNo IPR availablePANTHERPTHR31225:SF111TERPENE SYNTHASE 2-RELATEDcoord: 22..546
IPR001906Terpene synthase, N-terminal domainPFAMPF01397Terpene_synthcoord: 34..207
e-value: 9.5E-54
score: 182.0
IPR008949Isoprenoid synthase domain superfamilyGENE3D1.10.600.10Farnesyl Diphosphate Synthasecoord: 31..549
e-value: 2.6E-194
score: 648.4
IPR008949Isoprenoid synthase domain superfamilySUPERFAMILY48576Terpenoid synthasescoord: 234..560
IPR036965Terpene synthase, N-terminal domain superfamilyGENE3D1.50.10.130coord: 73..233
e-value: 2.6E-194
score: 648.4
IPR005630Terpene synthase, metal-binding domainPFAMPF03936Terpene_synth_Ccoord: 238..505
e-value: 5.7E-102
score: 340.6
IPR034741Terpene cyclase-like 1, C-terminal domainSFLDSFLDG01019Terpene_Cyclase_Like_1_C_Termcoord: 233..559
e-value: 0.0
score: 361.0
IPR044814Terpene cyclases, class 1, plantCDDcd00684Terpene_cyclase_plant_C1coord: 24..559
e-value: 0.0
score: 636.545
IPR008930Terpenoid cyclases/protein prenyltransferase alpha-alpha toroidSUPERFAMILY48239Terpenoid cyclases/Protein prenyltransferasescoord: 30..230

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg015772.1Spg015772.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016102 diterpenoid biosynthetic process
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0043565 sequence-specific DNA binding
molecular_function GO:0010333 terpene synthase activity
molecular_function GO:0016829 lyase activity