Spg015674 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg015674
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	U-box domain-containing protein
Location	scaffold10: 18394279 .. 18396168 (-)
RNA-Seq Expression	Spg015674
Synteny	Spg015674

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCATCGATGTTTCGTGAGTCGCCACCAACTGCCGTTTCTCACTGCCATCCGCAACCGCTCATGGTCGTCGAAGTCGGCTGGCTGGGTTTTGGTTTAAAGAATGCGTATTTTTCTTTTTTGAAAGAGCGTTAGTTCTATGTTTCCATTTTCGGACGAAGTGTCTTTGGAGGACAGAGTATTGTGAGAGTACTAGTTAGGGATATATTGGGATAATAGTAGACCTATATGGGGATAATAGTAGAATAGTAGTAAGGGCATTGTGGTAATTATGAGAATCTTTATTATAAATAGGAGAATGGGGAGCCTTAGAGGGTGGGATGATTTTGGGTATTGTCTCATAGTGGGACTTTGGTAGAGAGTTAGCTCTCTCGAAAAGCTATCGGTTTACATTGTAATTTCGTTTCATTATTGTAATATATTAGTATGTGGTGTTTTCTTAGTTTGGATACCTTACCCTCGTTTAAGTTCAATGTGTCAATTTCAATTTCTAAGCCAAGTTTTATTTCTTTCCTTTTTGTTTTCTATGCTATCTCTGCTGTGATGAAATGATGTGATGTGCTTTTTTCTAATAGTGCTATGTATTTAGGGGACTTGGATAGAAAATTTAGGCAGTTATTGACTTTATTTGGGTCGTACGTGCTGTTTCTGACTTGTTTATTTTTGGTTTTAAAGATTGTTCAAATATATGTTCATTTGGCAAGAGGTGATATGGAGAAAATTTTCCCAGCTGCCATTTCAAAGGATGGTCGATCATACAACGAGCAGGTAATTTATCTACACTAGCTTTGTGATAGATCCTTGAGAAGATAATTCTATTGAACTAAAAGGAAAAGAAAAAACCTGCTAGTATATGTAAACTCGAAATATATTTTATCATTGTGGATGTACTTGTTTGCATCTTTTTTCTGCCTGAATTTTGGAGCTTTCTCCAGTTGTTCAATGCTGCAGCAGTTGTACTTAAACGAATTGGAGAAGATGGAAGGATAATACAGGAATTTATTGATCTTGGTAACAAAGCGAAGGATGCAGCTTCTGAGGCCATGGATGCTGAAGCTACTCTAGGAGATATTCCGGATGAATTCCTTGACCCGATCCAAGTACGTTTAGCTCCAATTATTGTATGTTTAAGTTGATGGGTTTCAATCACTCTTTCGGATAGCCGAGTTATTGATTTCATTATGCATGATCGCTGTGCAAAAGAAGCTTTTCAACTGTGCCTCAAGAATGGAATGGTTTTGATTAGACAGAGATATTTAAAGCTTAAAAAAAGAAAATCAACGGATACTATGTTGGGACTAAACCAATTAAAACTTATCTTTCTTATGCAGTACACTCTAATGAAGGACCCTGTGATCTTACCTTCTTCAAGGATCACGGTCGACAGACCCGTCATTCAAAGACATCTCCTCAGCGATAGTGTAAGACTTGCCTTTCCTTTGAGTTGCATTTCTCTATCAAAACAAAGTGTTTGATTTTCTCTGCTAAACTTTACACGATTTGCAGACCGACCCTTTCAACCGATCTCATTTGACTTCGGACATGCTGATACCAAACGTAGAATTGAAAGCAAGAATTGAAGAATTTATCAGGTCACAAGAATTGAAGAGGCAACCCGACGGAGGGGTTGCGATGCAGAGCAGCAAGGCAACCATCCAACCCACAAGCGGAGAAATGTTGATTGATTAAAGTCGAGATGTAACTTAATCGCTCGTTATTTTATTGCTTTGGATACGAAATATTTTCGGAAGATATTTATATTTGATACAATGTTGTAAACTATAAAGACAGTGAAGATTATTTCTTTGGGCACTATTTAGTGCTTAGGCTGCCTAACCTTGTAATATTTGAAAATAAAGATCTTTAACTTATCAGGAAAGCAAAATTTGA

mRNA sequence

ATGGCATCGATGTTTCGTGAGTCGCCACCAACTGCCGTTTCTCACTGCCATCCGCAACCGCTCATGGTCGTCGAAATTGTTCAAATATATGTTCATTTGGCAAGAGGTGATATGGAGAAAATTTTCCCAGCTGCCATTTCAAAGGATGGTCGATCATACAACGAGCAGTTGTTCAATGCTGCAGCAGTTGTACTTAAACGAATTGGAGAAGATGGAAGGATAATACAGGAATTTATTGATCTTGGTAACAAAGCGAAGGATGCAGCTTCTGAGGCCATGGATGCTGAAGCTACTCTAGGAGATATTCCGGATGAATTCCTTGACCCGATCCAATACACTCTAATGAAGGACCCTGTGATCTTACCTTCTTCAAGGATCACGGTCGACAGACCCGTCATTCAAAGACATCTCCTCAGCGATAGTACCGACCCTTTCAACCGATCTCATTTGACTTCGGACATGCTGATACCAAACGTAGAATTGAAAGCAAGAATTGAAGAATTTATCAGGTCACAAGAATTGAAGAGGCAACCCGACGGAGGGGTTGCGATGCAGAGCAGCAAGGCAACCATCCAACCCACAAGCGGAGAAATGTTGATTGATTAA

Coding sequence (CDS)

ATGGCATCGATGTTTCGTGAGTCGCCACCAACTGCCGTTTCTCACTGCCATCCGCAACCGCTCATGGTCGTCGAAATTGTTCAAATATATGTTCATTTGGCAAGAGGTGATATGGAGAAAATTTTCCCAGCTGCCATTTCAAAGGATGGTCGATCATACAACGAGCAGTTGTTCAATGCTGCAGCAGTTGTACTTAAACGAATTGGAGAAGATGGAAGGATAATACAGGAATTTATTGATCTTGGTAACAAAGCGAAGGATGCAGCTTCTGAGGCCATGGATGCTGAAGCTACTCTAGGAGATATTCCGGATGAATTCCTTGACCCGATCCAATACACTCTAATGAAGGACCCTGTGATCTTACCTTCTTCAAGGATCACGGTCGACAGACCCGTCATTCAAAGACATCTCCTCAGCGATAGTACCGACCCTTTCAACCGATCTCATTTGACTTCGGACATGCTGATACCAAACGTAGAATTGAAAGCAAGAATTGAAGAATTTATCAGGTCACAAGAATTGAAGAGGCAACCCGACGGAGGGGTTGCGATGCAGAGCAGCAAGGCAACCATCCAACCCACAAGCGGAGAAATGTTGATTGATTAA

Protein sequence

MASMFRESPPTAVSHCHPQPLMVVEIVQIYVHLARGDMEKIFPAAISKDGRSYNEQLFNAAAVVLKRIGEDGRIIQEFIDLGNKAKDAASEAMDAEATLGDIPDEFLDPIQYTLMKDPVILPSSRITVDRPVIQRHLLSDSTDPFNRSHLTSDMLIPNVELKARIEEFIRSQELKRQPDGGVAMQSSKATIQPTSGEMLID

Homology

[Close]

BLAST of Spg015674 vs. NCBI nr
Match: KAG7022325.1 (putative ubiquitin conjugation factor E4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 325.1 bits (832), Expect = 4.3e-85
Identity = 166/180 (92.22%), Postives = 173/180 (96.11%), Query Frame = 0


Query: 22   MVVEIVQIYVHLARGDMEKIFPAAISKDGRSYNEQLFNAAAVVLKRIGEDGRIIQEFIDL 81
            ++ +IV IYVHLARGD E IFPAAISKDGRSYNEQLFNAAAVVL+RIGEDGRIIQEFI L
Sbjct: 861  LLKQIVHIYVHLARGDKENIFPAAISKDGRSYNEQLFNAAAVVLRRIGEDGRIIQEFIAL 920

Query: 82   GNKAKDAASEAMDAEATLGDIPDEFLDPIQYTLMKDPVILPSSRITVDRPVIQRHLLSDS 141
            GNKAKDAASEAMDAEATLGDIPDEFLDPIQYTLMKDPVILPSSRITVDRPVIQRHLLSDS
Sbjct: 921  GNKAKDAASEAMDAEATLGDIPDEFLDPIQYTLMKDPVILPSSRITVDRPVIQRHLLSDS 980

Query: 142  TDPFNRSHLTSDMLIPNVELKARIEEFIRSQELKRQPDGGVAMQSSKATIQPTSGEMLID 201
            TDPFNRSHLTSDMLIPN ELKARI+EFI S++LKRQPDGGVAMQSSKATIQPTSGEMLID
Sbjct: 981  TDPFNRSHLTSDMLIPNEELKARIKEFIMSKKLKRQPDGGVAMQSSKATIQPTSGEMLID 1040

[Close]

BLAST of Spg015674 vs. NCBI nr
Match: KAG6588496.1 (putative ubiquitin conjugation factor E4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 325.1 bits (832), Expect = 4.3e-85
Identity = 166/180 (92.22%), Postives = 173/180 (96.11%), Query Frame = 0


Query: 22   MVVEIVQIYVHLARGDMEKIFPAAISKDGRSYNEQLFNAAAVVLKRIGEDGRIIQEFIDL 81
            ++ +IV IYVHLARGD E IFPAAISKDGRSYNEQLFNAAAVVL+RIGEDGRIIQEFI L
Sbjct: 822  LLKQIVHIYVHLARGDKENIFPAAISKDGRSYNEQLFNAAAVVLRRIGEDGRIIQEFIAL 881

Query: 82   GNKAKDAASEAMDAEATLGDIPDEFLDPIQYTLMKDPVILPSSRITVDRPVIQRHLLSDS 141
            GNKAKDAASEAMDAEATLGDIPDEFLDPIQYTLMKDPVILPSSRITVDRPVIQRHLLSDS
Sbjct: 882  GNKAKDAASEAMDAEATLGDIPDEFLDPIQYTLMKDPVILPSSRITVDRPVIQRHLLSDS 941

Query: 142  TDPFNRSHLTSDMLIPNVELKARIEEFIRSQELKRQPDGGVAMQSSKATIQPTSGEMLID 201
            TDPFNRSHLTSDMLIPN ELKARI+EFI S++LKRQPDGGVAMQSSKATIQPTSGEMLID
Sbjct: 942  TDPFNRSHLTSDMLIPNEELKARIKEFIMSKKLKRQPDGGVAMQSSKATIQPTSGEMLID 1001

[Close]

BLAST of Spg015674 vs. NCBI nr
Match: XP_022932900.1 (probable ubiquitin conjugation factor E4 [Cucurbita moschata])

HSP 1 Score: 325.1 bits (832), Expect = 4.3e-85
Identity = 166/180 (92.22%), Postives = 173/180 (96.11%), Query Frame = 0


Query: 22   MVVEIVQIYVHLARGDMEKIFPAAISKDGRSYNEQLFNAAAVVLKRIGEDGRIIQEFIDL 81
            ++ +IV IYVHLARGD E IFPAAISKDGRSYNEQLFNAAAVVL+RIGEDGRIIQEFI L
Sbjct: 861  LLKQIVHIYVHLARGDKENIFPAAISKDGRSYNEQLFNAAAVVLRRIGEDGRIIQEFIAL 920

Query: 82   GNKAKDAASEAMDAEATLGDIPDEFLDPIQYTLMKDPVILPSSRITVDRPVIQRHLLSDS 141
            GNKAKDAASEAMDAEATLGDIPDEFLDPIQYTLMKDPVILPSSRITVDRPVIQRHLLSDS
Sbjct: 921  GNKAKDAASEAMDAEATLGDIPDEFLDPIQYTLMKDPVILPSSRITVDRPVIQRHLLSDS 980

Query: 142  TDPFNRSHLTSDMLIPNVELKARIEEFIRSQELKRQPDGGVAMQSSKATIQPTSGEMLID 201
            TDPFNRSHLTSDMLIPN ELKARI+EFI S++LKRQPDGGVAMQSSKATIQPTSGEMLID
Sbjct: 981  TDPFNRSHLTSDMLIPNEELKARIKEFIMSKKLKRQPDGGVAMQSSKATIQPTSGEMLID 1040

[Close]

BLAST of Spg015674 vs. NCBI nr
Match: XP_022969252.1 (probable ubiquitin conjugation factor E4 [Cucurbita maxima])

HSP 1 Score: 325.1 bits (832), Expect = 4.3e-85
Identity = 166/180 (92.22%), Postives = 173/180 (96.11%), Query Frame = 0


Query: 22   MVVEIVQIYVHLARGDMEKIFPAAISKDGRSYNEQLFNAAAVVLKRIGEDGRIIQEFIDL 81
            ++ +IV IYVHLARGD E IFPAAISKDGRSYNEQLFNAAAVVL+RIGEDGRIIQEFI L
Sbjct: 863  LLKQIVHIYVHLARGDKENIFPAAISKDGRSYNEQLFNAAAVVLRRIGEDGRIIQEFIAL 922

Query: 82   GNKAKDAASEAMDAEATLGDIPDEFLDPIQYTLMKDPVILPSSRITVDRPVIQRHLLSDS 141
            GNKAKDAASEAMDAEATLGDIPDEFLDPIQYTLMKDPVILPSSRITVDRPVIQRHLLSDS
Sbjct: 923  GNKAKDAASEAMDAEATLGDIPDEFLDPIQYTLMKDPVILPSSRITVDRPVIQRHLLSDS 982

Query: 142  TDPFNRSHLTSDMLIPNVELKARIEEFIRSQELKRQPDGGVAMQSSKATIQPTSGEMLID 201
            TDPFNRSHLTSDMLIPN ELKARI+EFI S++LKRQPDGGVAMQSSKATIQPTSGEMLID
Sbjct: 983  TDPFNRSHLTSDMLIPNEELKARIKEFIMSKKLKRQPDGGVAMQSSKATIQPTSGEMLID 1042

[Close]

BLAST of Spg015674 vs. NCBI nr
Match: XP_038903925.1 (probable ubiquitin conjugation factor E4 isoform X1 [Benincasa hispida])

HSP 1 Score: 323.6 bits (828), Expect = 1.2e-84
Identity = 166/180 (92.22%), Postives = 171/180 (95.00%), Query Frame = 0


Query: 22   MVVEIVQIYVHLARGDMEKIFPAAISKDGRSYNEQLFNAAAVVLKRIGEDGRIIQEFIDL 81
            ++ +IV IYVHLARGD E IFPAAISKDGRSYNEQLF AAAVVL RIGEDGRIIQEFIDL
Sbjct: 862  LLKQIVHIYVHLARGDSENIFPAAISKDGRSYNEQLFTAAAVVLNRIGEDGRIIQEFIDL 921

Query: 82   GNKAKDAASEAMDAEATLGDIPDEFLDPIQYTLMKDPVILPSSRITVDRPVIQRHLLSDS 141
            GNKAKDAASEAMDAEATLGDIPDEFLDPIQYTLMKDPVILPSSRITVDRPVIQRHLLSDS
Sbjct: 922  GNKAKDAASEAMDAEATLGDIPDEFLDPIQYTLMKDPVILPSSRITVDRPVIQRHLLSDS 981

Query: 142  TDPFNRSHLTSDMLIPNVELKARIEEFIRSQELKRQPDGGVAMQSSKATIQPTSGEMLID 201
            TDPFNRSHLT+DMLIPN ELKARIEEFIRSQELK+Q DGGVAMQSSKATIQPT GEMLID
Sbjct: 982  TDPFNRSHLTADMLIPNEELKARIEEFIRSQELKKQLDGGVAMQSSKATIQPTIGEMLID 1041

[Close]

BLAST of Spg015674 vs. ExPASy Swiss-Prot
Match: Q9LF41 (Probable ubiquitin conjugation factor E4 OS=Arabidopsis thaliana OX=3702 GN=PUB1 PE=2 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 5.5e-67
Identity = 128/180 (71.11%), Postives = 155/180 (86.11%), Query Frame = 0


Query: 22   MVVEIVQIYVHLARGDMEKIFPAAISKDGRSYNEQLFNAAAVVLKRIGEDGRIIQEFIDL 81
            ++ +IV+IYV+LARGD   IFP AIS DGRSYNEQLFNA A VL+RIGE+GRIIQEF++L
Sbjct: 861  LLKQIVRIYVNLARGDTVNIFPGAISSDGRSYNEQLFNAGADVLRRIGEEGRIIQEFMEL 920

Query: 82   GNKAKDAASEAMDAEATLGDIPDEFLDPIQYTLMKDPVILPSSRITVDRPVIQRHLLSDS 141
            G KAK AASEA+DAEA LG+IPDEFLDPIQYTLM+DPVILPSSRITVDRP+IQRHLLSD+
Sbjct: 921  GTKAKAAASEALDAEAALGEIPDEFLDPIQYTLMRDPVILPSSRITVDRPIIQRHLLSDN 980

Query: 142  TDPFNRSHLTSDMLIPNVELKARIEEFIRSQELKRQPDGGVAMQSSKATIQPTSGEMLID 201
             DPFNR+HLTSDMLIP++ELKA+I+EF++S + K++  G     S+K  IQ T+ +MLID
Sbjct: 981  HDPFNRAHLTSDMLIPDIELKAKIDEFVKSHQSKKRTSG--EDSSNKERIQTTNSDMLID 1038

[Close]

BLAST of Spg015674 vs. ExPASy Swiss-Prot
Match: A5PKG6 (Ubiquitin conjugation factor E4 A OS=Bos taurus OX=9913 GN=UBE4A PE=2 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 2.0e-32
Identity = 83/162 (51.23%), Postives = 102/162 (62.96%), Query Frame = 0


Query: 18   PQPLMVVEIVQIYVHLARGDMEKIFPAAISKDGRSYNEQLFNAAAVVLKRIGEDGRIIQE 77
            PQ L V +I  IY++L  GD E+ F A + KDGRSY+  LF     VLK+I + G +I  
Sbjct: 908  PQQL-VSDICTIYLNL--GD-EENFCATVPKDGRSYSPTLFAQTVRVLKKINKPGNMIVA 967

Query: 78   FIDLGNKAKDAASEAMDAEATLGDIPDEFLDPIQYTLMKDPVILPSSRITVDRPVIQRHL 137
            F +L  + K  A      E T  D  DEFLDPI  TLM DPV+LPSSR+TVDR  I RHL
Sbjct: 968  FSNLAERIKSLADLQQQEEETYADACDEFLDPIMSTLMSDPVVLPSSRVTVDRSTIARHL 1027

Query: 138  LSDSTDPFNRSHLTSDMLIPNVELKARIEEFI--RSQELKRQ 178
            LSD TDPFNRS LT D + PN ELK +I+ ++  R Q+ K Q
Sbjct: 1028 LSDQTDPFNRSPLTMDQIRPNTELKEKIQRWLAERKQQQKEQ 1065

[Close]

BLAST of Spg015674 vs. ExPASy Swiss-Prot
Match: E9Q735 (Ubiquitin conjugation factor E4 A OS=Mus musculus OX=10090 GN=Ube4a PE=1 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 2.0e-32
Identity = 82/163 (50.31%), Postives = 103/163 (63.19%), Query Frame = 0


Query: 18   PQPLMVVEIVQIYVHLARGDMEKIFPAAISKDGRSYNEQLFNAAAVVLKRIGEDGRIIQE 77
            PQ L V +I  IY++L  GD E+ F A + KDGRSY+  LF     VLK+I + G +I  
Sbjct: 870  PQQL-VSDICTIYLNL--GD-EENFCATVPKDGRSYSPTLFAQTVRVLKKINKPGNMIVA 929

Query: 78   FIDLGNKAKDAASEAMDAEATLGDIPDEFLDPIQYTLMKDPVILPSSRITVDRPVIQRHL 137
            F +L  + K  A      E T  D  DEFLDPI  TLM DPV+LPSSR+TVDR  I RHL
Sbjct: 930  FSNLAERIKSLADLQQQEEETYADACDEFLDPIMSTLMSDPVVLPSSRVTVDRSTIARHL 989

Query: 138  LSDSTDPFNRSHLTSDMLIPNVELKARIEEFI-RSQELKRQPD 180
            LSD TDPFNRS LT D + PN ELK +I+ ++   ++ K QP+
Sbjct: 990  LSDQTDPFNRSPLTMDQIRPNTELKEKIQRWLAERKQQKEQPE 1028

[Close]

BLAST of Spg015674 vs. ExPASy Swiss-Prot
Match: Q14139 (Ubiquitin conjugation factor E4 A OS=Homo sapiens OX=9606 GN=UBE4A PE=1 SV=2)

HSP 1 Score: 139.0 bits (349), Expect = 5.7e-32
Identity = 79/160 (49.38%), Postives = 102/160 (63.75%), Query Frame = 0


Query: 18   PQPLMVVEIVQIYVHLARGDMEKIFPAAISKDGRSYNEQLFNAAAVVLKRIGEDGRIIQE 77
            PQ L V +I  IY++L  GD E+ F A + KDGRSY+  LF     VLK+I + G +I  
Sbjct: 908  PQQL-VSDICTIYLNL--GD-EENFCATVPKDGRSYSPTLFAQTVRVLKKINKPGNMIMA 967

Query: 78   FIDLGNKAKDAASEAMDAEATLGDIPDEFLDPIQYTLMKDPVILPSSRITVDRPVIQRHL 137
            F +L  + K  A      E T  D  DEFLDPI  TLM DPV+LPSSR+TVDR  I RHL
Sbjct: 968  FSNLAERIKSLADLQQQEEETYADACDEFLDPIMSTLMCDPVVLPSSRVTVDRSTIARHL 1027

Query: 138  LSDSTDPFNRSHLTSDMLIPNVELKARIEEFIRSQELKRQ 178
            LSD TDPFNRS LT D + PN ELK +I+ ++  ++ +++
Sbjct: 1028 LSDQTDPFNRSPLTMDQIRPNTELKEKIQRWLAERKQQKE 1063

[Close]

BLAST of Spg015674 vs. ExPASy Swiss-Prot
Match: Q5R9G3 (Ubiquitin conjugation factor E4 A OS=Pongo abelii OX=9601 GN=UBE4A PE=2 SV=2)

HSP 1 Score: 138.3 bits (347), Expect = 9.7e-32
Identity = 79/160 (49.38%), Postives = 102/160 (63.75%), Query Frame = 0


Query: 18   PQPLMVVEIVQIYVHLARGDMEKIFPAAISKDGRSYNEQLFNAAAVVLKRIGEDGRIIQE 77
            PQ L V +I  IY++L  GD E+ F A + KDGRSY+  LF     VLK+I + G +I  
Sbjct: 908  PQQL-VSDICTIYLNL--GD-EENFCATVPKDGRSYSPTLFAQTVRVLKKINKPGNMIVA 967

Query: 78   FIDLGNKAKDAASEAMDAEATLGDIPDEFLDPIQYTLMKDPVILPSSRITVDRPVIQRHL 137
            F +L  + K  A      E T  D  DEFLDPI  TLM DPV+LPSSR+TVDR  I RHL
Sbjct: 968  FSNLAERIKSLADLQQQEEETYADACDEFLDPIMSTLMCDPVVLPSSRVTVDRSTIARHL 1027

Query: 138  LSDSTDPFNRSHLTSDMLIPNVELKARIEEFIRSQELKRQ 178
            LSD TDPFNRS LT D + PN ELK +I+ ++  ++ +++
Sbjct: 1028 LSDQTDPFNRSPLTMDQIRPNTELKEKIQRWLAERKQQKE 1063

[Close]

BLAST of Spg015674 vs. ExPASy TrEMBL
Match: A0A6J1HVU6 (probable ubiquitin conjugation factor E4 OS=Cucurbita maxima OX=3661 GN=LOC111468307 PE=3 SV=1)

HSP 1 Score: 325.1 bits (832), Expect = 2.1e-85
Identity = 166/180 (92.22%), Postives = 173/180 (96.11%), Query Frame = 0


Query: 22   MVVEIVQIYVHLARGDMEKIFPAAISKDGRSYNEQLFNAAAVVLKRIGEDGRIIQEFIDL 81
            ++ +IV IYVHLARGD E IFPAAISKDGRSYNEQLFNAAAVVL+RIGEDGRIIQEFI L
Sbjct: 863  LLKQIVHIYVHLARGDKENIFPAAISKDGRSYNEQLFNAAAVVLRRIGEDGRIIQEFIAL 922

Query: 82   GNKAKDAASEAMDAEATLGDIPDEFLDPIQYTLMKDPVILPSSRITVDRPVIQRHLLSDS 141
            GNKAKDAASEAMDAEATLGDIPDEFLDPIQYTLMKDPVILPSSRITVDRPVIQRHLLSDS
Sbjct: 923  GNKAKDAASEAMDAEATLGDIPDEFLDPIQYTLMKDPVILPSSRITVDRPVIQRHLLSDS 982

Query: 142  TDPFNRSHLTSDMLIPNVELKARIEEFIRSQELKRQPDGGVAMQSSKATIQPTSGEMLID 201
            TDPFNRSHLTSDMLIPN ELKARI+EFI S++LKRQPDGGVAMQSSKATIQPTSGEMLID
Sbjct: 983  TDPFNRSHLTSDMLIPNEELKARIKEFIMSKKLKRQPDGGVAMQSSKATIQPTSGEMLID 1042

[Close]

BLAST of Spg015674 vs. ExPASy TrEMBL
Match: A0A6J1F3F7 (probable ubiquitin conjugation factor E4 OS=Cucurbita moschata OX=3662 GN=LOC111439459 PE=3 SV=1)

HSP 1 Score: 325.1 bits (832), Expect = 2.1e-85
Identity = 166/180 (92.22%), Postives = 173/180 (96.11%), Query Frame = 0


Query: 22   MVVEIVQIYVHLARGDMEKIFPAAISKDGRSYNEQLFNAAAVVLKRIGEDGRIIQEFIDL 81
            ++ +IV IYVHLARGD E IFPAAISKDGRSYNEQLFNAAAVVL+RIGEDGRIIQEFI L
Sbjct: 861  LLKQIVHIYVHLARGDKENIFPAAISKDGRSYNEQLFNAAAVVLRRIGEDGRIIQEFIAL 920

Query: 82   GNKAKDAASEAMDAEATLGDIPDEFLDPIQYTLMKDPVILPSSRITVDRPVIQRHLLSDS 141
            GNKAKDAASEAMDAEATLGDIPDEFLDPIQYTLMKDPVILPSSRITVDRPVIQRHLLSDS
Sbjct: 921  GNKAKDAASEAMDAEATLGDIPDEFLDPIQYTLMKDPVILPSSRITVDRPVIQRHLLSDS 980

Query: 142  TDPFNRSHLTSDMLIPNVELKARIEEFIRSQELKRQPDGGVAMQSSKATIQPTSGEMLID 201
            TDPFNRSHLTSDMLIPN ELKARI+EFI S++LKRQPDGGVAMQSSKATIQPTSGEMLID
Sbjct: 981  TDPFNRSHLTSDMLIPNEELKARIKEFIMSKKLKRQPDGGVAMQSSKATIQPTSGEMLID 1040

[Close]

BLAST of Spg015674 vs. ExPASy TrEMBL
Match: A0A6J1CTD2 (probable ubiquitin conjugation factor E4 OS=Momordica charantia OX=3673 GN=LOC111014159 PE=3 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 1.0e-84
Identity = 167/180 (92.78%), Postives = 172/180 (95.56%), Query Frame = 0


Query: 22   MVVEIVQIYVHLARGDMEKIFPAAISKDGRSYNEQLFNAAAVVLKRIGEDGRIIQEFIDL 81
            ++ +IV IYVHLARGD EKIFPAAISKDGRSYNEQLFNAAA VL RIGEDGRIIQEFIDL
Sbjct: 859  LLKQIVHIYVHLARGDTEKIFPAAISKDGRSYNEQLFNAAAAVLGRIGEDGRIIQEFIDL 918

Query: 82   GNKAKDAASEAMDAEATLGDIPDEFLDPIQYTLMKDPVILPSSRITVDRPVIQRHLLSDS 141
            GNKAKDAASEAMDAEATLGDIPDEFLDPIQYTLMKDPVILPSSRITVDRPVIQRHLLSDS
Sbjct: 919  GNKAKDAASEAMDAEATLGDIPDEFLDPIQYTLMKDPVILPSSRITVDRPVIQRHLLSDS 978

Query: 142  TDPFNRSHLTSDMLIPNVELKARIEEFIRSQELKRQPDGGVAMQSSKATIQPTSGEMLID 201
            TDPFNRSHLTSDMLIPN ELKARIEEFIRSQELKR+P+GGVAMQ  KATIQPTSGEMLID
Sbjct: 979  TDPFNRSHLTSDMLIPNDELKARIEEFIRSQELKRKPEGGVAMQ--KATIQPTSGEMLID 1036

[Close]

BLAST of Spg015674 vs. ExPASy TrEMBL
Match: A0A5A7UCZ1 (Putative ubiquitin conjugation factor E4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G007670 PE=3 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 2.8e-82
Identity = 163/181 (90.06%), Postives = 170/181 (93.92%), Query Frame = 0


Query: 22   MVVEIVQIYVHLARGDMEKIFPAAISKDGRSYNEQLFNAAAVVLKRIGEDGRIIQEFIDL 81
            ++ +IV IYVHLARGD E IFPAAISKDGRSYNEQLF AAA VL+RIGED RIIQEF DL
Sbjct: 863  LLKQIVHIYVHLARGDTENIFPAAISKDGRSYNEQLFTAAAAVLRRIGEDSRIIQEFTDL 922

Query: 82   GNKAKDAASEAMDAEATLGDIPDEFLDPIQYTLMKDPVILPSSRITVDRPVIQRHLLSDS 141
            GNKAKDAASEAMDAEATLGDIPDEFLDPIQYTLMKDPVILPSSRITVDRPVIQRHLLSDS
Sbjct: 923  GNKAKDAASEAMDAEATLGDIPDEFLDPIQYTLMKDPVILPSSRITVDRPVIQRHLLSDS 982

Query: 142  TDPFNRSHLTSDMLIPNVELKARIEEFIRSQELKRQPD-GGVAMQSSKATIQPTSGEMLI 201
            TDPFNRSHLT+DMLIPN ELKARI+EFIRSQELK+Q D GGVAMQSSKATIQPTSGEMLI
Sbjct: 983  TDPFNRSHLTADMLIPNEELKARIKEFIRSQELKKQLDGGGVAMQSSKATIQPTSGEMLI 1042

[Close]

BLAST of Spg015674 vs. ExPASy TrEMBL
Match: A0A6J1J877 (probable ubiquitin conjugation factor E4 OS=Cucurbita maxima OX=3661 GN=LOC111482236 PE=3 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 2.8e-82
Identity = 162/181 (89.50%), Postives = 172/181 (95.03%), Query Frame = 0


Query: 22   MVVEIVQIYVHLARGDMEKIFPAAISKDGRSYNEQLFNAAAVVLKRIGEDGRIIQEFIDL 81
            ++ +IV +YVHLARGD + IFPAAISKDGRSYNEQLFNAAAVVL+RIGEDGRIIQEFI L
Sbjct: 860  LLKQIVHMYVHLARGDTDNIFPAAISKDGRSYNEQLFNAAAVVLRRIGEDGRIIQEFIAL 919

Query: 82   GNKAKDAASEAMDAEATLGDIPDEFLDPIQYTLMKDPVILPSSRITVDRPVIQRHLLSDS 141
            GNKAKDAASEAMDAEATLGDIPDEFLDPIQYTLMKDPVILPSSRITVDRPVIQRHLLSDS
Sbjct: 920  GNKAKDAASEAMDAEATLGDIPDEFLDPIQYTLMKDPVILPSSRITVDRPVIQRHLLSDS 979

Query: 142  TDPFNRSHLTSDMLIPNVELKARIEEFIRSQELKRQPD-GGVAMQSSKATIQPTSGEMLI 201
            TDPFNRSHLT+DMLIPN ELKARI+EFIRSQELK+Q D GGVAMQSSK TIQPT+GEMLI
Sbjct: 980  TDPFNRSHLTADMLIPNEELKARIKEFIRSQELKKQLDGGGVAMQSSKTTIQPTNGEMLI 1039

[Close]

BLAST of Spg015674 vs. TAIR 10
Match: AT5G15400.1 (U-box domain-containing protein )

HSP 1 Score: 255.4 bits (651), Expect = 3.9e-68
Identity = 128/180 (71.11%), Postives = 155/180 (86.11%), Query Frame = 0


Query: 22   MVVEIVQIYVHLARGDMEKIFPAAISKDGRSYNEQLFNAAAVVLKRIGEDGRIIQEFIDL 81
            ++ +IV+IYV+LARGD   IFP AIS DGRSYNEQLFNA A VL+RIGE+GRIIQEF++L
Sbjct: 861  LLKQIVRIYVNLARGDTVNIFPGAISSDGRSYNEQLFNAGADVLRRIGEEGRIIQEFMEL 920

Query: 82   GNKAKDAASEAMDAEATLGDIPDEFLDPIQYTLMKDPVILPSSRITVDRPVIQRHLLSDS 141
            G KAK AASEA+DAEA LG+IPDEFLDPIQYTLM+DPVILPSSRITVDRP+IQRHLLSD+
Sbjct: 921  GTKAKAAASEALDAEAALGEIPDEFLDPIQYTLMRDPVILPSSRITVDRPIIQRHLLSDN 980

Query: 142  TDPFNRSHLTSDMLIPNVELKARIEEFIRSQELKRQPDGGVAMQSSKATIQPTSGEMLID 201
             DPFNR+HLTSDMLIP++ELKA+I+EF++S + K++  G     S+K  IQ T+ +MLID
Sbjct: 981  HDPFNRAHLTSDMLIPDIELKAKIDEFVKSHQSKKRTSG--EDSSNKERIQTTNSDMLID 1038

[Close]

BLAST of Spg015674 vs. TAIR 10
Match: AT2G28830.1 (PLANT U-BOX 12 )

HSP 1 Score: 51.2 bits (121), Expect = 1.1e-06
Identity = 41/147 (27.89%), Postives = 73/147 (49.66%), Query Frame = 0


Query: 46  ISKDGRSYNEQLFNAAAVVLKRIGEDGRIIQEFI-DLGNKAKDAASEAMDAEATLGDIPD 105
           +S  G     + F   ++VLK+I +  +     + D   + K +  ++ D +  +   P+
Sbjct: 199 VSSSGGDDPGESFEKMSMVLKKIKDFVQTYNPNLDDAPLRLKSSLPKSRDDDRDMLIPPE 258

Query: 106 EFLDPIQYTLMKDPVILPSSRITVDRPVIQRHLLSDS-TDPFNRSHLTSDMLIPNVELKA 165
           EF  PI   LM DPVI+ S + T +R  I++ L     T P  +  LTSD++ PN  L++
Sbjct: 259 EFRCPISLELMTDPVIVSSGQ-TYERECIKKWLEGGHLTCPKTQETLTSDIMTPNYVLRS 318

Query: 166 RIEEFIRSQELKRQPDGGVAMQSSKAT 191
            I ++  S  ++      ++  SSKA+
Sbjct: 319 LIAQWCESNGIEPPKRPNISQPSSKAS 344

[Close]

BLAST of Spg015674 vs. TAIR 10
Match: AT3G46510.1 (plant U-box 13 )

HSP 1 Score: 45.4 bits (106), Expect = 6.1e-05
Identity = 36/120 (30.00%), Postives = 63/120 (52.50%), Query Frame = 0


Query: 62  AVVLKRI-----GEDGRIIQEFIDLGNKAKDAASEAMDAEATLGDIPDEFLDPIQYTLMK 121
           A+VLK I      ED    ++ + + +++    S A  A   +  IPD+F  PI   +M+
Sbjct: 213 AMVLKMIKDFVQTEDDNGEEQKVGVNSRSNGQTSTA--ASQKIPVIPDDFRCPISLEMMR 272

Query: 122 DPVILPSSRITVDRPVIQRHLL-SDSTDPFNRSHLTSDMLIPNVELKARIEEFIRSQELK 176
           DPVI+ S + T +R  I++ +    ST P  +  LTS  L PN  L++ I ++  + +++
Sbjct: 273 DPVIVSSGQ-TYERTCIEKWIEGGHSTCPKTQQALTSTTLTPNYVLRSLIAQWCEANDIE 329

[Close]

BLAST of Spg015674 vs. TAIR 10
Match: AT1G66160.1 (CYS, MET, PRO, and GLY protein 1 )

HSP 1 Score: 43.1 bits (100), Expect = 3.0e-04
Identity = 29/66 (43.94%), Postives = 38/66 (57.58%), Query Frame = 0


Query: 102 IPDEFLDPIQYTLMKDPVILPSSRITVDRPVIQRHLLSD-STDPFNRSHLTSDMLIPNVE 161
           IP +F  PI Y LMKDPVI+ +S IT DR  I++   S   T P   + LTS   IPN  
Sbjct: 33  IPSQFQCPISYELMKDPVII-ASGITYDRENIEKWFESGYQTCPVTNTVLTSLEQIPNHT 92

Query: 162 LKARIE 167
           ++  I+
Sbjct: 93  IRRMIQ 97

[Close]

BLAST of Spg015674 vs. TAIR 10
Match: AT1G71020.1 (ARM repeat superfamily protein )

HSP 1 Score: 42.0 bits (97), Expect = 6.7e-04
Identity = 31/100 (31.00%), Postives = 54/100 (54.00%), Query Frame = 0


Query: 102 IPDEFLDPIQYTLMKDPVILPSSRITVDRPVIQRHL-LSDSTDPFNRSHLTSDMLIPNVE 161
           IP++FL PI   LMKDP I+ + + T +R  IQR +   + + P  +  L +  L PN  
Sbjct: 243 IPEDFLCPISLELMKDPAIVSTGQ-TYERSFIQRWIDCGNLSCPKTQQKLENFTLTPNYV 302

Query: 162 LKARIEEFIRSQELKRQPDGGV--AMQSSKATIQPTSGEM 199
           L++ I ++     ++ QP G +    ++S  + +  SG+M
Sbjct: 303 LRSLISQWCTKHNIE-QPGGYMNGRTKNSDGSFRDLSGDM 340

The following BLAST results are available for this feature:

BLAST of Spg015674 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7022325.1	4.3e-85	92.22	putative ubiquitin conjugation factor E4 [Cucurbita argyrosperma subsp. argyrosp...	[more]
KAG6588496.1	4.3e-85	92.22	putative ubiquitin conjugation factor E4, partial [Cucurbita argyrosperma subsp....	[more]
XP_022932900.1	4.3e-85	92.22	probable ubiquitin conjugation factor E4 [Cucurbita moschata]	[more]
XP_022969252.1	4.3e-85	92.22	probable ubiquitin conjugation factor E4 [Cucurbita maxima]	[more]
XP_038903925.1	1.2e-84	92.22	probable ubiquitin conjugation factor E4 isoform X1 [Benincasa hispida]	[more]

BLAST of Spg015674 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LF41	5.5e-67	71.11	Probable ubiquitin conjugation factor E4 OS=Arabidopsis thaliana OX=3702 GN=PUB1...	[more]
A5PKG6	2.0e-32	51.23	Ubiquitin conjugation factor E4 A OS=Bos taurus OX=9913 GN=UBE4A PE=2 SV=1	[more]
E9Q735	2.0e-32	50.31	Ubiquitin conjugation factor E4 A OS=Mus musculus OX=10090 GN=Ube4a PE=1 SV=1	[more]
Q14139	5.7e-32	49.38	Ubiquitin conjugation factor E4 A OS=Homo sapiens OX=9606 GN=UBE4A PE=1 SV=2	[more]
Q5R9G3	9.7e-32	49.38	Ubiquitin conjugation factor E4 A OS=Pongo abelii OX=9601 GN=UBE4A PE=2 SV=2	[more]

BLAST of Spg015674 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HVU6	2.1e-85	92.22	probable ubiquitin conjugation factor E4 OS=Cucurbita maxima OX=3661 GN=LOC11146...	[more]
A0A6J1F3F7	2.1e-85	92.22	probable ubiquitin conjugation factor E4 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1CTD2	1.0e-84	92.78	probable ubiquitin conjugation factor E4 OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A5A7UCZ1	2.8e-82	90.06	Putative ubiquitin conjugation factor E4 OS=Cucumis melo var. makuwa OX=1194695 ...	[more]
A0A6J1J877	2.8e-82	89.50	probable ubiquitin conjugation factor E4 OS=Cucurbita maxima OX=3661 GN=LOC11148...	[more]

BLAST of Spg015674 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G15400.1	3.9e-68	71.11	U-box domain-containing protein	[more]
AT2G28830.1	1.1e-06	27.89	PLANT U-BOX 12	[more]
AT3G46510.1	6.1e-05	30.00	plant U-box 13	[more]
AT1G66160.1	3.0e-04	43.94	CYS, MET, PRO, and GLY protein 1	[more]
AT1G71020.1	6.7e-04	31.00	ARM repeat superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003613	U box domain	SMART	SM00504	Ubox_2	coord: 105..168 e-value: 1.5E-25 score: 100.9
IPR003613	U box domain	PFAM	PF04564	U-box	coord: 102..172 e-value: 7.7E-32 score: 109.3
IPR003613	U box domain	PROSITE	PS51698	U_BOX	coord: 101..175 score: 33.801186
IPR019474	Ubiquitin conjugation factor E4, core	PFAM	PF10408	Ufd2P_core	coord: 22..85 e-value: 1.9E-11 score: 43.3
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 101..179 e-value: 4.5E-29 score: 102.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 176..201
None	No IPR available	PANTHER	PTHR13931:SF15	SUBFAMILY NOT NAMED	coord: 23..176
None	No IPR available	CDD	cd16657	RING-Ubox_UBE4A	coord: 104..172 e-value: 5.09709E-43 score: 136.971
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 94..174
IPR045132	Ubiquitin conjugation factor E4	PANTHER	PTHR13931	UBIQUITINATION FACTOR E4	coord: 23..176

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg015674.1	Spg015674.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0030433	ubiquitin-dependent ERAD pathway
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0000151	ubiquitin ligase complex
molecular_function	GO:0034450	ubiquitin-ubiquitin ligase activity
molecular_function	GO:0004842	ubiquitin-protein transferase activity