Spg015467 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg015467
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Ribonuclease T(2)
Location	scaffold10: 13663836 .. 13665077 (+)
RNA-Seq Expression	Spg015467
Synteny	Spg015467

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAACTTGAACGTTTTGGACGAGGGAGCAATGGCGATGGCGAAAAGAGAAATTGTCCTTGTGTTTGTCTTAACCATTTTGTTTCCAATGGTTAAATCACAAACTTTCGATAACTTTTGGTTCGTTCAACACTGGCCACCAGCTGTCTGCAGCTTCCAACAAGGACGATGTGTAGGACGAGGGCTACGAAGCTTCACAATCCATGGCGTGTGGCCTCAAAAGGGAGGAACCTCCGTAATTAATTGTCCTGGTACTACCTTTGATTTCACCAAGGTAAAATTGAAACAACTAAAGTCTCTCATTTTTCTTCATCAAGCATGCATTGATTCTACTCCAATGAACAAATATGACCATGATAACTTATAAATTTGACATAATAGTTAATGAATCGACACCAACTGAGTAGCAACATCAAGCCACCTAAGAGAAGAAAGCTCAAATTAATTTCAACTATCTTCTAAGAAATTTAGAAGAATTAGAAATTTTTCTTTCTTAGATAGCCTGATTTCTTCTTTAAGTTTCTCTCCATTTACTAAATATTATGTCACATGCTAGTTTACCATTAGCCAATTTTAATATCACAAGATGATGAGAATATACTCAAAAATGCTATGACATTAGTCAATAAGGACTAAATTGAAAAACCACCCACCATTACAAACAAAAACACATATTTAGAGAGAGAAAAAAAAAACTACTTTTGACTACATAGAGTTCTTTGGCTTTCAGGTTTCACATTTAGAAAGCAGTCTAAACGTAGTTTGGCCGAATTTGATAACGGGCAACAACAAATGGTTTTGGGGTCATGAGTGGAACAAGCATGGGATTTGCTCAGAAAGCAAGTTCGACCAGCAAGCATACTTCCAAATGGCCATAAATATGAGGAACAGTATTGACCTTCTTGGTGCCCTCAGAGTTGGTGGATTAGCACCAAACGGACGCAACAAGTCGAGGCAAAGAGTGCAAAGTGCCATAAGAGCGCAATTCGGGAAGGAACCAGTTCTTCGCTGTAGAGGAACTGGCGGACAATCTCGATTGCTGGAGATTGTGATGTGCTTTGATGATGATGGTGTCACCCTCATCAACTGCAATCCTGCTAGTTCAAATTGCCCCAACAGCTTCATCTTTTAAAAACTCTTCAAATTTTATGCAATCAACAAGATATGTTGTTATCTTGAAATAAGTTTATGCTATAGTTCACAAGGTCGTTTGCATAACATTTTAGAGTTGTTTTTATATTTTAA

mRNA sequence

ATGGCGATGGCGAAAAGAGAAATTGTCCTTGTGTTTGTCTTAACCATTTTGTTTCCAATGGTTAAATCACAAACTTTCGATAACTTTTGGTTCGTTCAACACTGGCCACCAGCTGTCTGCAGCTTCCAACAAGGACGATGTGTAGGACGAGGGCTACGAAGCTTCACAATCCATGGCGTGTGGCCTCAAAAGGGAGGAACCTCCGTAATTAATTGTCCTGGTACTACCTTTGATTTCACCAAGGTTTCACATTTAGAAAGCAGTCTAAACGTAGTTTGGCCGAATTTGATAACGGGCAACAACAAATGGTTTTGGGGTCATGAGTGGAACAAGCATGGGATTTGCTCAGAAAGCAAGTTCGACCAGCAAGCATACTTCCAAATGGCCATAAATATGAGGAACAGTATTGACCTTCTTGGTGCCCTCAGAGTTGGTGGATTAGCACCAAACGGACGCAACAAGTCGAGGCAAAGAGTGCAAAGTGCCATAAGAGCGCAATTCGGGAAGGAACCAGTTCTTCGCTGTAGAGGAACTGGCGGACAATCTCGATTGCTGGAGATTGTGATGTGCTTTGATGATGATGGTGTCACCCTCATCAACTGCAATCCTGCTAGTTCAAATTGCCCCAACAGCTTCATCTTTTAA

Coding sequence (CDS)

ATGGCGATGGCGAAAAGAGAAATTGTCCTTGTGTTTGTCTTAACCATTTTGTTTCCAATGGTTAAATCACAAACTTTCGATAACTTTTGGTTCGTTCAACACTGGCCACCAGCTGTCTGCAGCTTCCAACAAGGACGATGTGTAGGACGAGGGCTACGAAGCTTCACAATCCATGGCGTGTGGCCTCAAAAGGGAGGAACCTCCGTAATTAATTGTCCTGGTACTACCTTTGATTTCACCAAGGTTTCACATTTAGAAAGCAGTCTAAACGTAGTTTGGCCGAATTTGATAACGGGCAACAACAAATGGTTTTGGGGTCATGAGTGGAACAAGCATGGGATTTGCTCAGAAAGCAAGTTCGACCAGCAAGCATACTTCCAAATGGCCATAAATATGAGGAACAGTATTGACCTTCTTGGTGCCCTCAGAGTTGGTGGATTAGCACCAAACGGACGCAACAAGTCGAGGCAAAGAGTGCAAAGTGCCATAAGAGCGCAATTCGGGAAGGAACCAGTTCTTCGCTGTAGAGGAACTGGCGGACAATCTCGATTGCTGGAGATTGTGATGTGCTTTGATGATGATGGTGTCACCCTCATCAACTGCAATCCTGCTAGTTCAAATTGCCCCAACAGCTTCATCTTTTAA

Protein sequence

MAMAKREIVLVFVLTILFPMVKSQTFDNFWFVQHWPPAVCSFQQGRCVGRGLRSFTIHGVWPQKGGTSVINCPGTTFDFTKVSHLESSLNVVWPNLITGNNKWFWGHEWNKHGICSESKFDQQAYFQMAINMRNSIDLLGALRVGGLAPNGRNKSRQRVQSAIRAQFGKEPVLRCRGTGGQSRLLEIVMCFDDDGVTLINCNPASSNCPNSFIF

Homology

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BLAST of Spg015467 vs. NCBI nr
Match: BAA10891.1 (ribonuclease (RNase LC1) [Luffa aegyptiaca])

HSP 1 Score: 425.6 bits (1093), Expect = 2.5e-115
Identity = 197/214 (92.06%), Postives = 206/214 (96.26%), Query Frame = 0


Query: 1   MAMAKREIVLVFVLTILFPMVKSQTFDNFWFVQHWPPAVCSFQQGRCVGRGLRSFTIHGV 60
           MAMAKREIVLVFVLTILFPMVKSQTFD+FW VQHWPPAVCSFQQGRCVG+GLRSFTIHGV
Sbjct: 1   MAMAKREIVLVFVLTILFPMVKSQTFDSFWMVQHWPPAVCSFQQGRCVGQGLRSFTIHGV 60

Query: 61  WPQKGGTSVINCPGTTFDFTKVSHLESSLNVVWPNLITGNNKWFWGHEWNKHGICSESKF 120
           WPQKGGTSVINCPG TFDFTK+SHLES+LNV WPN+ITGNNKWFWGHEWNKHGICS SKF
Sbjct: 61  WPQKGGTSVINCPGPTFDFTKISHLESTLNVDWPNVITGNNKWFWGHEWNKHGICSVSKF 120

Query: 121 DQQAYFQMAINMRNSIDLLGALRVGGLAPNGRNKSRQRVQSAIRAQFGKEPVLRCRGTGG 180
           DQQAYFQMAINMRNSIDLL ALRVGG+ PNGR+K+RQRVQSAIRAQ GKEPVLRCRGTG 
Sbjct: 121 DQQAYFQMAINMRNSIDLLSALRVGGVVPNGRSKARQRVQSAIRAQLGKEPVLRCRGTGR 180

Query: 181 QSRLLEIVMCFDDDGVTLINCNPASSNCPNSFIF 215
           QSRLLEIVMCFDDDGVTLINCNPA+SNCPNSFIF
Sbjct: 181 QSRLLEIVMCFDDDGVTLINCNPANSNCPNSFIF 214

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BLAST of Spg015467 vs. NCBI nr
Match: XP_022142280.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 388.3 bits (996), Expect = 4.4e-104
Identity = 177/216 (81.94%), Postives = 198/216 (91.67%), Query Frame = 0


Query: 1   MAMAKREIVLVFVL--TILFPMVKSQTFDNFWFVQHWPPAVCSFQQGRCVGRGLRSFTIH 60
           M ++KRE+VL+F L  TI FP+VKSQ FDNFWFVQ WPPAVC+FQQGRCVG+GLR+FT+H
Sbjct: 5   MVISKREVVLMFALTITIFFPVVKSQAFDNFWFVQQWPPAVCNFQQGRCVGQGLRTFTVH 64

Query: 61  GVWPQKGGTSVINCPGTTFDFTKVSHLESSLNVVWPNLITGNNKWFWGHEWNKHGICSES 120
           G+WPQKGGTSVINC GT FDFT+VSHL++ LN VWPN+ITGNN++FWGHEWNKHGICSES
Sbjct: 65  GLWPQKGGTSVINCSGTRFDFTQVSHLQNDLNRVWPNVITGNNRFFWGHEWNKHGICSES 124

Query: 121 KFDQQAYFQMAINMRNSIDLLGALRVGGLAPNGRNKSRQRVQSAIRAQFGKEPVLRCRGT 180
           KFD +AYFQMAINMR+S+DL+ ALRVGGLAPNGR KSRQRVQSAIRAQFGKEPVLRCRGT
Sbjct: 125 KFDMKAYFQMAINMRDSLDLMNALRVGGLAPNGRTKSRQRVQSAIRAQFGKEPVLRCRGT 184

Query: 181 GGQSRLLEIVMCFDDDGVTLINCNPASSNCPNSFIF 215
           G   RL+EIVMCFDDDGVTLINCNPASSNC NSFIF
Sbjct: 185 GRNVRLIEIVMCFDDDGVTLINCNPASSNCANSFIF 220

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BLAST of Spg015467 vs. NCBI nr
Match: XP_022155091.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 375.9 bits (964), Expect = 2.3e-100
Identity = 171/216 (79.17%), Postives = 193/216 (89.35%), Query Frame = 0


Query: 1   MAMAKREIVLVFVL--TILFPMVKSQTFDNFWFVQHWPPAVCSFQQGRCVGRGLRSFTIH 60
           M  ++REIV +FVL  TI  P+VKSQTFD+FWFVQ WPPAVCSFQQGRCVG+GLR+FTIH
Sbjct: 1   MKTSRREIVFMFVLAITIFLPIVKSQTFDDFWFVQQWPPAVCSFQQGRCVGQGLRTFTIH 60

Query: 61  GVWPQKGGTSVINCPGTTFDFTKVSHLESSLNVVWPNLITGNNKWFWGHEWNKHGICSES 120
           GVWPQKGGTSV+NCPGT FDFTK+S LE+ LN VWPN+ITG+NK+FW HEWNKHG+CSES
Sbjct: 61  GVWPQKGGTSVVNCPGTPFDFTKISSLETDLNRVWPNVITGDNKFFWSHEWNKHGVCSES 120

Query: 121 KFDQQAYFQMAINMRNSIDLLGALRVGGLAPNGRNKSRQRVQSAIRAQFGKEPVLRCRGT 180
           KF+ +AYFQMAINMRNSIDL+ AL+VGGLAPNG +KS+QRVQSA+R  FGKEPVLRCRGT
Sbjct: 121 KFNMKAYFQMAINMRNSIDLMNALKVGGLAPNGLSKSKQRVQSALRTHFGKEPVLRCRGT 180

Query: 181 GGQSRLLEIVMCFDDDGVTLINCNPASSNCPNSFIF 215
           G   RL+EIVMCFDDDGVTLINCNP SSNC NSFIF
Sbjct: 181 GSNIRLIEIVMCFDDDGVTLINCNPVSSNCANSFIF 216

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BLAST of Spg015467 vs. NCBI nr
Match: XP_008443402.1 (PREDICTED: ribonuclease MC-like [Cucumis melo])

HSP 1 Score: 345.1 bits (884), Expect = 4.3e-91
Identity = 156/216 (72.22%), Postives = 181/216 (83.80%), Query Frame = 0


Query: 1   MAMAKREIV-LVFVLTILFPMVKSQTFDNFWFVQHWPPAVCSFQQGRCVGRGLRSFTIHG 60
           M   KR++V  VFVLTILFP+VKSQTFDNFWFVQ WPPA+C+ Q GRCVGRG RSFTIHG
Sbjct: 1   MEKTKRDVVFFVFVLTILFPIVKSQTFDNFWFVQQWPPAICTLQSGRCVGRGTRSFTIHG 60

Query: 61  VWPQKGGTSVINCPGTTFDFTKVSHLESSLNVVWPNLITGNNKWFWGHEWNKHGICSESK 120
           +WPQKGG SV NC G +FDFTK++HLES LNVVWPN++TGNNK+FWGHEWNKHGICSESK
Sbjct: 61  LWPQKGGRSVTNCSGNSFDFTKIAHLESDLNVVWPNVVTGNNKFFWGHEWNKHGICSESK 120

Query: 121 FDQQAYFQMAINMRNSIDLLGALRVGGLAPNGRNKSRQRVQSAIRAQFGKEPVLRCR-GT 180
           FDQ  YFQ AINMR+SIDLL  LR GG+ PNG +K++QRV++AI + F K+P+LRC+  +
Sbjct: 121 FDQAKYFQTAINMRHSIDLLSVLRTGGIVPNGASKAKQRVETAISSHFAKDPILRCKTAS 180

Query: 181 GGQSRLLEIVMCFDDDGVTLINCNPASSNCPNSFIF 215
            GQ  L EIV+CFDDDGVTLINCN A SNC  SFIF
Sbjct: 181 NGQVLLTEIVICFDDDGVTLINCNKARSNCARSFIF 216

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BLAST of Spg015467 vs. NCBI nr
Match: XP_038905534.1 (ribonuclease MC-like [Benincasa hispida])

HSP 1 Score: 344.7 bits (883), Expect = 5.6e-91
Identity = 156/216 (72.22%), Postives = 183/216 (84.72%), Query Frame = 0


Query: 1   MAMAKREIV-LVFVLTILFPMVKSQTFDNFWFVQHWPPAVCSFQQGRCVGRGLRSFTIHG 60
           MA  KR IV LVF LTILFP+V+SQTFD+FWFVQ WPPAVC+ Q GRCVGRG RSFTIHG
Sbjct: 1   MATTKRNIVLLVFGLTILFPIVQSQTFDDFWFVQQWPPAVCALQSGRCVGRGTRSFTIHG 60

Query: 61  VWPQKGGTSVINCPGTTFDFTKVSHLESSLNVVWPNLITGNNKWFWGHEWNKHGICSESK 120
           +WPQKGG+SV NC G +FDFTK++HLE+ LN+VWPN+ITGNNK+FWGHEWNKHGICSESK
Sbjct: 61  LWPQKGGSSVTNCAGNSFDFTKIAHLENDLNIVWPNVITGNNKFFWGHEWNKHGICSESK 120

Query: 121 FDQQAYFQMAINMRNSIDLLGALRVGGLAPNGRNKSRQRVQSAIRAQFGKEPVLRCR-GT 180
           FDQ AYFQ AINMR+SIDLL  +R GG+ PNG +K++QRV+S I + FGK+P+LRC+  +
Sbjct: 121 FDQAAYFQTAINMRHSIDLLSVMRTGGVIPNGASKAKQRVESVISSHFGKDPILRCKTAS 180

Query: 181 GGQSRLLEIVMCFDDDGVTLINCNPASSNCPNSFIF 215
            GQ  L E+VMCFDDDGVTL+NCN A SNC  SFIF
Sbjct: 181 NGQVLLTEVVMCFDDDGVTLLNCNKARSNCATSFIF 216

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BLAST of Spg015467 vs. ExPASy Swiss-Prot
Match: P23540 (Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 6.7e-63
Identity = 111/194 (57.22%), Postives = 143/194 (73.71%), Query Frame = 0


Query: 26  FDNFWFVQHWPPAVCSFQQ-GRCVGRGLRSFTIHGVWPQKGGTSVINCP-GTTFDFTKVS 85
           FD+FWFVQ WPPAVCSFQ+ G C G GLR+FTIHG+WPQ  GTS+ NCP G+ FD TK+S
Sbjct: 1   FDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFTIHGLWPQGSGTSLTNCPQGSPFDITKIS 60

Query: 86  HLESSLNVVWPNLITGNNKWFWGHEWNKHGICSESKFDQQAYFQMAINMRNSIDLLGALR 145
           HL+S LN +WPN++  NN+ FW HEW KHG CSES F+Q AYF++A++MRN+ D++GALR
Sbjct: 61  HLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYDIIGALR 120

Query: 146 VGGLAPNGRNKSRQRVQSAIRAQFGKEPVLRCRGTGGQSR---LLEIVMCFDDDGVTLIN 205
                PNGR KSRQ ++  ++A+FGK P LRCR T  Q++   L+++V CF  DG TLI+
Sbjct: 121 PHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCR-TDPQTKVSYLVQVVACFAQDGSTLID 180

Query: 206 CNPASSNCPNSFIF 215
           C      C  +FIF
Sbjct: 181 C--TRDTCGANFIF 191

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BLAST of Spg015467 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 7.7e-35
Identity = 77/201 (38.31%), Postives = 113/201 (56.22%), Query Frame = 0


Query: 9   VLVFVLTILFPMVKSQTFDNFWFVQHWPPAVCSFQQGRCV---GRGLRSFTIHGVWPQ-K 68
           +LV + ++      S+ FD F+FVQ WP + C  Q+  C    G+    F IHG+WP  K
Sbjct: 13  LLVILPSVFSASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGIHGLWPNYK 72

Query: 69  GGTSVINCPGT-TFDFTKVSHLESSLNVVWPNLI--TGNNKWFWGHEWNKHGICSESKFD 128
            GT   NC  +  FD + +S L +S+   WP L   +G+ + FW HEW KHG CSES  D
Sbjct: 73  DGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSESVID 132

Query: 129 QQAYFQMAINMRNSIDLLGALRVGGLAPNGRNKSRQRVQSAIRAQFGKEPVLRCRGTG-G 188
           Q  YFQ A+N++   +LLGAL   G+ P+G++ S + ++ +I+   G  P + C   G G
Sbjct: 133 QHEYFQTALNLKQKTNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVECNRDGSG 192

Query: 189 QSRLLEIVMCFDDDGVTLINC 202
            S+L ++ +C D  G  LI C
Sbjct: 193 NSQLYQVYLCVDRSGSGLIEC 213

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BLAST of Spg015467 vs. ExPASy Swiss-Prot
Match: P80022 (Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2)

HSP 1 Score: 140.6 bits (353), Expect = 2.1e-32
Identity = 72/203 (35.47%), Postives = 115/203 (56.65%), Query Frame = 0


Query: 8   IVLVFVLTILFPMVKSQTFDNFWFVQHWPPAVCSFQQGRC---VGRGLRSFTIHGVWPQK 67
           ++L+ +   L  +  ++ FD F+FVQ WP + C  +Q  C    G+    F IHG+WP  
Sbjct: 11  LILLIITQCLSVLNAAKDFDFFYFVQQWPGSYCDTKQSCCYPTTGKPAADFGIHGLWPNN 70

Query: 68  G-GTSVINC-PGTTFDFTKVSHLESSLNVVWPNLI--TGNNKWFWGHEWNKHGICSESKF 127
             GT   NC P + +D +++S L SS+   WP L   +G+   FW HEW KHG C+ES  
Sbjct: 71  NDGTYPSNCDPNSPYDQSQISDLISSMQQNWPTLACPSGSGSTFWSHEWEKHGTCAESVL 130

Query: 128 -DQQAYFQMAINMRNSIDLLGALRVGGLAPNGRNKSRQRVQSAIRAQFGKEPVLRCR-GT 187
            +Q AYF+ A++++N IDLL  L+   + P+G +     +++AI++  G  P ++C    
Sbjct: 131 TNQHAYFKKALDLKNQIDLLSILQGADIHPDGESYDLVNIRNAIKSAIGYTPWIQCNVDQ 190

Query: 188 GGQSRLLEIVMCFDDDGVTLINC 202
            G S+L ++ +C D  G +LI C
Sbjct: 191 SGNSQLYQVYICVDGSGSSLIEC 213

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BLAST of Spg015467 vs. ExPASy Swiss-Prot
Match: P42815 (Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 9.1e-28
Identity = 69/201 (34.33%), Postives = 101/201 (50.25%), Query Frame = 0


Query: 9   VLVFVLTILFPMVKSQTFDNFWFVQHWPPAVCSFQQGRC---VGRGLRSFTIHGVWPQ-K 68
           + +  L  L+    +Q FD F+FV  WP A C  +   C    G+    F IHG+WP  K
Sbjct: 5   IFILALQQLYVQSFAQDFDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLWPNYK 64

Query: 69  GGTSVINC-PGTTFDFTKVSHLESSLNVVWPNLITGNNKW--FWGHEWNKHGICSESKFD 128
            G    NC P + FD  +VS L S L   WP L   +N    FW HEW KHG C+ES+ D
Sbjct: 65  TGGWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESELD 124

Query: 129 QQAYFQMAINMRNSIDLLGALRVGGLAPNGRNKSRQRVQSAIRAQFGKEPVLRC-RGTGG 188
           Q  YF+  + ++   +LL AL   G+ P+ +    + +++ I+   G  P + C + +  
Sbjct: 125 QHDYFEAGLKLKQKANLLHALTNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECNKDSSH 184

Query: 189 QSRLLEIVMCFDDDGVTLINC 202
            S+L +I +C D      INC
Sbjct: 185 NSQLYQIYLCVDTSASKFINC 205

[Close]

BLAST of Spg015467 vs. ExPASy Swiss-Prot
Match: P80196 (Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2)

HSP 1 Score: 114.0 bits (284), Expect = 2.1e-24
Identity = 71/218 (32.57%), Postives = 107/218 (49.08%), Query Frame = 0


Query: 8   IVLVFVLTILFPMVKSQTFDNFWFVQHWPPAVCSFQQGRC---VGRGLRSFTIHGVWPQ- 67
           I ++ V  +L   V SQ FD F+FVQ WP + C  ++  C    G+    F+IHG+WP  
Sbjct: 10  IKIIVVQCLLVLCVTSQDFDFFYFVQQWPASYCDTRRSCCYPTTGKPDEDFSIHGLWPNY 69

Query: 68  KGGTSVINCP-GTTFDFTKVSHLESSLNVVWPNLI--TGNNKWFWGHEWNKHGICSESKF 127
           K G    NC   ++ D ++ S L S++   WP+L   + +   FW HEW KHG C  S  
Sbjct: 70  KDGKWPQNCDRESSLDESEFSDLISTMEKNWPSLACPSSDGLKFWSHEWLKHGTC--SAL 129

Query: 128 DQQAYFQMAINMRNSIDLLGALRVGGLAP-NGRNKSRQRVQSAIRAQFGKEPVLRCR-GT 187
           +Q AYFQ A++ +   +LL  L   G+ P NG     + ++ AI    G  P + C   +
Sbjct: 130 NQHAYFQTALDFKTKSNLLQNLNNAGIKPRNGDYYGVESIKKAIEKGVGHTPFIECNVDS 189

Query: 188 GGQSRLLEIVMCFDDDGVTLINCN--PASSNCPNSFIF 215
            G  +L ++ +C D      I+C   P    C +   F
Sbjct: 190 QGNHQLYQVYLCVDSSASKFIDCPIFPHGGKCGSKIEF 225

[Close]

BLAST of Spg015467 vs. ExPASy TrEMBL
Match: Q40115 (Ribonuclease (RNase LC1) OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 425.6 bits (1093), Expect = 1.2e-115
Identity = 197/214 (92.06%), Postives = 206/214 (96.26%), Query Frame = 0


Query: 1   MAMAKREIVLVFVLTILFPMVKSQTFDNFWFVQHWPPAVCSFQQGRCVGRGLRSFTIHGV 60
           MAMAKREIVLVFVLTILFPMVKSQTFD+FW VQHWPPAVCSFQQGRCVG+GLRSFTIHGV
Sbjct: 1   MAMAKREIVLVFVLTILFPMVKSQTFDSFWMVQHWPPAVCSFQQGRCVGQGLRSFTIHGV 60

Query: 61  WPQKGGTSVINCPGTTFDFTKVSHLESSLNVVWPNLITGNNKWFWGHEWNKHGICSESKF 120
           WPQKGGTSVINCPG TFDFTK+SHLES+LNV WPN+ITGNNKWFWGHEWNKHGICS SKF
Sbjct: 61  WPQKGGTSVINCPGPTFDFTKISHLESTLNVDWPNVITGNNKWFWGHEWNKHGICSVSKF 120

Query: 121 DQQAYFQMAINMRNSIDLLGALRVGGLAPNGRNKSRQRVQSAIRAQFGKEPVLRCRGTGG 180
           DQQAYFQMAINMRNSIDLL ALRVGG+ PNGR+K+RQRVQSAIRAQ GKEPVLRCRGTG 
Sbjct: 121 DQQAYFQMAINMRNSIDLLSALRVGGVVPNGRSKARQRVQSAIRAQLGKEPVLRCRGTGR 180

Query: 181 QSRLLEIVMCFDDDGVTLINCNPASSNCPNSFIF 215
           QSRLLEIVMCFDDDGVTLINCNPA+SNCPNSFIF
Sbjct: 181 QSRLLEIVMCFDDDGVTLINCNPANSNCPNSFIF 214

[Close]

BLAST of Spg015467 vs. ExPASy TrEMBL
Match: A0A6J1CKH7 (Ribonuclease T(2) OS=Momordica charantia OX=3673 GN=LOC111012440 PE=3 SV=1)

HSP 1 Score: 388.3 bits (996), Expect = 2.1e-104
Identity = 177/216 (81.94%), Postives = 198/216 (91.67%), Query Frame = 0


Query: 1   MAMAKREIVLVFVL--TILFPMVKSQTFDNFWFVQHWPPAVCSFQQGRCVGRGLRSFTIH 60
           M ++KRE+VL+F L  TI FP+VKSQ FDNFWFVQ WPPAVC+FQQGRCVG+GLR+FT+H
Sbjct: 5   MVISKREVVLMFALTITIFFPVVKSQAFDNFWFVQQWPPAVCNFQQGRCVGQGLRTFTVH 64

Query: 61  GVWPQKGGTSVINCPGTTFDFTKVSHLESSLNVVWPNLITGNNKWFWGHEWNKHGICSES 120
           G+WPQKGGTSVINC GT FDFT+VSHL++ LN VWPN+ITGNN++FWGHEWNKHGICSES
Sbjct: 65  GLWPQKGGTSVINCSGTRFDFTQVSHLQNDLNRVWPNVITGNNRFFWGHEWNKHGICSES 124

Query: 121 KFDQQAYFQMAINMRNSIDLLGALRVGGLAPNGRNKSRQRVQSAIRAQFGKEPVLRCRGT 180
           KFD +AYFQMAINMR+S+DL+ ALRVGGLAPNGR KSRQRVQSAIRAQFGKEPVLRCRGT
Sbjct: 125 KFDMKAYFQMAINMRDSLDLMNALRVGGLAPNGRTKSRQRVQSAIRAQFGKEPVLRCRGT 184

Query: 181 GGQSRLLEIVMCFDDDGVTLINCNPASSNCPNSFIF 215
           G   RL+EIVMCFDDDGVTLINCNPASSNC NSFIF
Sbjct: 185 GRNVRLIEIVMCFDDDGVTLINCNPASSNCANSFIF 220

[Close]

BLAST of Spg015467 vs. ExPASy TrEMBL
Match: A0A6J1DQN7 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111022224 PE=3 SV=1)

HSP 1 Score: 375.9 bits (964), Expect = 1.1e-100
Identity = 171/216 (79.17%), Postives = 193/216 (89.35%), Query Frame = 0


Query: 1   MAMAKREIVLVFVL--TILFPMVKSQTFDNFWFVQHWPPAVCSFQQGRCVGRGLRSFTIH 60
           M  ++REIV +FVL  TI  P+VKSQTFD+FWFVQ WPPAVCSFQQGRCVG+GLR+FTIH
Sbjct: 1   MKTSRREIVFMFVLAITIFLPIVKSQTFDDFWFVQQWPPAVCSFQQGRCVGQGLRTFTIH 60

Query: 61  GVWPQKGGTSVINCPGTTFDFTKVSHLESSLNVVWPNLITGNNKWFWGHEWNKHGICSES 120
           GVWPQKGGTSV+NCPGT FDFTK+S LE+ LN VWPN+ITG+NK+FW HEWNKHG+CSES
Sbjct: 61  GVWPQKGGTSVVNCPGTPFDFTKISSLETDLNRVWPNVITGDNKFFWSHEWNKHGVCSES 120

Query: 121 KFDQQAYFQMAINMRNSIDLLGALRVGGLAPNGRNKSRQRVQSAIRAQFGKEPVLRCRGT 180
           KF+ +AYFQMAINMRNSIDL+ AL+VGGLAPNG +KS+QRVQSA+R  FGKEPVLRCRGT
Sbjct: 121 KFNMKAYFQMAINMRNSIDLMNALKVGGLAPNGLSKSKQRVQSALRTHFGKEPVLRCRGT 180

Query: 181 GGQSRLLEIVMCFDDDGVTLINCNPASSNCPNSFIF 215
           G   RL+EIVMCFDDDGVTLINCNP SSNC NSFIF
Sbjct: 181 GSNIRLIEIVMCFDDDGVTLINCNPVSSNCANSFIF 216

[Close]

BLAST of Spg015467 vs. ExPASy TrEMBL
Match: A0A1S3B8Q3 (Ribonuclease T(2) OS=Cucumis melo OX=3656 GN=LOC103486996 PE=3 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 2.1e-91
Identity = 156/216 (72.22%), Postives = 181/216 (83.80%), Query Frame = 0


Query: 1   MAMAKREIV-LVFVLTILFPMVKSQTFDNFWFVQHWPPAVCSFQQGRCVGRGLRSFTIHG 60
           M   KR++V  VFVLTILFP+VKSQTFDNFWFVQ WPPA+C+ Q GRCVGRG RSFTIHG
Sbjct: 1   MEKTKRDVVFFVFVLTILFPIVKSQTFDNFWFVQQWPPAICTLQSGRCVGRGTRSFTIHG 60

Query: 61  VWPQKGGTSVINCPGTTFDFTKVSHLESSLNVVWPNLITGNNKWFWGHEWNKHGICSESK 120
           +WPQKGG SV NC G +FDFTK++HLES LNVVWPN++TGNNK+FWGHEWNKHGICSESK
Sbjct: 61  LWPQKGGRSVTNCSGNSFDFTKIAHLESDLNVVWPNVVTGNNKFFWGHEWNKHGICSESK 120

Query: 121 FDQQAYFQMAINMRNSIDLLGALRVGGLAPNGRNKSRQRVQSAIRAQFGKEPVLRCR-GT 180
           FDQ  YFQ AINMR+SIDLL  LR GG+ PNG +K++QRV++AI + F K+P+LRC+  +
Sbjct: 121 FDQAKYFQTAINMRHSIDLLSVLRTGGIVPNGASKAKQRVETAISSHFAKDPILRCKTAS 180

Query: 181 GGQSRLLEIVMCFDDDGVTLINCNPASSNCPNSFIF 215
            GQ  L EIV+CFDDDGVTLINCN A SNC  SFIF
Sbjct: 181 NGQVLLTEIVICFDDDGVTLINCNKARSNCARSFIF 216

[Close]

BLAST of Spg015467 vs. ExPASy TrEMBL
Match: A0A0A0LC37 (Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G825050 PE=3 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 1.1e-89
Identity = 151/208 (72.60%), Postives = 175/208 (84.13%), Query Frame = 0


Query: 8   IVLVFVLTILFPMVKSQTFDNFWFVQHWPPAVCSFQQGRCVGRGLRSFTIHGVWPQKGGT 67
           +  VFVLTILFP+VKSQTFD+FWFVQ WPPAVC+ Q GRCVGRG RSFTIHG+WPQKGG 
Sbjct: 10  VFFVFVLTILFPIVKSQTFDDFWFVQQWPPAVCTLQSGRCVGRGTRSFTIHGLWPQKGGR 69

Query: 68  SVINCPGTTFDFTKVSHLESSLNVVWPNLITGNNKWFWGHEWNKHGICSESKFDQQAYFQ 127
           SV NC G  FDFTK++HLE+ LNVVWPN++TGNNK+FWGHEWNKHGICSESKFD+  YFQ
Sbjct: 70  SVTNCTGNQFDFTKIAHLENDLNVVWPNVVTGNNKFFWGHEWNKHGICSESKFDEAKYFQ 129

Query: 128 MAINMRNSIDLLGALRVGGLAPNGRNKSRQRVQSAIRAQFGKEPVLRC-RGTGGQSRLLE 187
            AINMR+ IDLL  LR GG+ PNG +K++QRV++AI + FGK+P+LRC + + GQ  L E
Sbjct: 130 TAINMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKASNGQVLLTE 189

Query: 188 IVMCFDDDGVTLINCNPASSNCPNSFIF 215
           IVMCFDDDGVTLINCN A SNC  SFIF
Sbjct: 190 IVMCFDDDGVTLINCNKARSNCAGSFIF 217

[Close]

BLAST of Spg015467 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 148.7 bits (374), Expect = 5.4e-36
Identity = 77/201 (38.31%), Postives = 113/201 (56.22%), Query Frame = 0


Query: 9   VLVFVLTILFPMVKSQTFDNFWFVQHWPPAVCSFQQGRCV---GRGLRSFTIHGVWPQ-K 68
           +LV + ++      S+ FD F+FVQ WP + C  Q+  C    G+    F IHG+WP  K
Sbjct: 13  LLVILPSVFSASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGIHGLWPNYK 72

Query: 69  GGTSVINCPGT-TFDFTKVSHLESSLNVVWPNLI--TGNNKWFWGHEWNKHGICSESKFD 128
            GT   NC  +  FD + +S L +S+   WP L   +G+ + FW HEW KHG CSES  D
Sbjct: 73  DGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSESVID 132

Query: 129 QQAYFQMAINMRNSIDLLGALRVGGLAPNGRNKSRQRVQSAIRAQFGKEPVLRCRGTG-G 188
           Q  YFQ A+N++   +LLGAL   G+ P+G++ S + ++ +I+   G  P + C   G G
Sbjct: 133 QHEYFQTALNLKQKTNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVECNRDGSG 192

Query: 189 QSRLLEIVMCFDDDGVTLINC 202
            S+L ++ +C D  G  LI C
Sbjct: 193 NSQLYQVYLCVDRSGSGLIEC 213

[Close]

BLAST of Spg015467 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 125.9 bits (315), Expect = 3.8e-29
Identity = 73/216 (33.80%), Postives = 109/216 (50.46%), Query Frame = 0


Query: 8   IVLVFVLTILFPMVKSQTFDNFWFVQHWPPAVCSFQQGRCV---GRGLRSFTIHGVWPQ- 67
           ++ + V   LF     + FD F+FV  WP A C   +  C    G+    F IHG+WP  
Sbjct: 10  LLKLLVFQGLFVSRPQEDFDFFYFVLQWPGAYCDTSRACCYPTSGKPAADFGIHGLWPNY 69

Query: 68  KGGTSVINC-PGTTFDFTKVSHLESSLNVVWPNLITGNNKW--FWGHEWNKHGICSESKF 127
            GG+   NC P + FD +++S L SSL   WP L   +N+   FW HEW KHG CSES  
Sbjct: 70  NGGSWPSNCDPDSQFDRSQISDLVSSLKKNWPTLSCPSNEGFNFWEHEWEKHGTCSESVM 129

Query: 128 DQQAYFQMAINMRNSIDLLGALRVGGLAPNGRNKSRQRVQSAIRAQFGKEPVLRC-RGTG 187
           DQ  YF+ A+ ++   +LL  L+  G+ P+    +  ++ +AI+   G  P + C +   
Sbjct: 130 DQHEYFENALKLKQKANLLQILKNSGINPDDGFYNLDKITNAIKDGIGFTPGIECNKDPE 189

Query: 188 GQSRLLEIVMCFDDDGVTLINCNP-ASSNCPNSFIF 215
             ++L +I +C D  G   I C      +CP+   F
Sbjct: 190 RNAQLHQIYICVDTSGTEFIECPVLPRGSCPSQIQF 225

[Close]

BLAST of Spg015467 vs. TAIR 10
Match: AT1G26820.1 (ribonuclease 3 )

HSP 1 Score: 125.2 bits (313), Expect = 6.5e-29
Identity = 69/201 (34.33%), Postives = 101/201 (50.25%), Query Frame = 0


Query: 9   VLVFVLTILFPMVKSQTFDNFWFVQHWPPAVCSFQQGRC---VGRGLRSFTIHGVWPQ-K 68
           + +  L  L+    +Q FD F+FV  WP A C  +   C    G+    F IHG+WP  K
Sbjct: 5   IFILALQQLYVQSFAQDFDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLWPNYK 64

Query: 69  GGTSVINC-PGTTFDFTKVSHLESSLNVVWPNLITGNNKW--FWGHEWNKHGICSESKFD 128
            G    NC P + FD  +VS L S L   WP L   +N    FW HEW KHG C+ES+ D
Sbjct: 65  TGGWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESELD 124

Query: 129 QQAYFQMAINMRNSIDLLGALRVGGLAPNGRNKSRQRVQSAIRAQFGKEPVLRC-RGTGG 188
           Q  YF+  + ++   +LL AL   G+ P+ +    + +++ I+   G  P + C + +  
Sbjct: 125 QHDYFEAGLKLKQKANLLHALTNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECNKDSSH 184

Query: 189 QSRLLEIVMCFDDDGVTLINC 202
            S+L +I +C D      INC
Sbjct: 185 NSQLYQIYLCVDTSASKFINC 205

[Close]

BLAST of Spg015467 vs. TAIR 10
Match: AT1G14210.1 (Ribonuclease T2 family protein )

HSP 1 Score: 117.5 bits (293), Expect = 1.3e-26
Identity = 68/210 (32.38%), Postives = 101/210 (48.10%), Query Frame = 0


Query: 6   REIVLVFVLTILFPMVKSQ----TFDNFWFVQHWPPAVCSFQQGRC---VGRGLRSFTIH 65
           R I++V +L +   +V S      F+ F++V +WP A+C  Q+G C    G     F IH
Sbjct: 2   RGIIIVSLLILQSLVVSSSQTEPDFNFFYWVNYWPGAICDSQKGCCPPTKGNTASDFIIH 61

Query: 66  GVWPQ-KGGTSVINCPGTT-FDFTKVSHLESSLNVVWPN----LITGNNKWFWGHEWNKH 125
           G+WPQ   GT    C  T  FD +K+S L   +   W          N    W HEWNKH
Sbjct: 62  GLWPQFNNGTWPAFCDQTNLFDISKISDLVCQMEKKWTEWGVWACPSNETNLWEHEWNKH 121

Query: 126 GICSESKFDQQAYFQMAINMRNSIDLLGALRVGGLAPNGRNKSRQRVQSAIRAQFGKEPV 185
           G C +S FDQ +YF+  +  ++ + LL  L   G+ PN    S   +++AI+   G  P 
Sbjct: 122 GTCVQSIFDQHSYFRTNLKFKHKVHLLNILIQKGIKPNDGFYSLDEIKNAIKCAIGFAPG 181

Query: 186 LRC-RGTGGQSRLLEIVMCFDDDGVTLINC 202
           + C     G  +L +I +C D+     + C
Sbjct: 182 IECNEDVKGNKQLFQIYICLDNYAKEFVEC 211

[Close]

BLAST of Spg015467 vs. TAIR 10
Match: AT2G39780.1 (ribonuclease 2 )

HSP 1 Score: 73.9 bits (180), Expect = 1.7e-13
Identity = 64/219 (29.22%), Postives = 90/219 (41.10%), Query Frame = 0


Query: 3   MAKREIVLVFVLTI-------LFPMVKSQ-TFDNFWFVQHWPPAVCSFQQGRCVGRGL-- 62
           MA R  +L+ V  I       +  + +SQ  FD F     WP   C   +  C       
Sbjct: 1   MASRLCLLLLVACIAGAFAGDVIELNRSQREFDYFALSLQWPGTYCRGTRHCCSKNACCR 60

Query: 63  -----RSFTIHGVWPQ-KGGTSVINCPGTTFDFTKVSHLESSLNVVWPNL-------ITG 122
                  FTIHG+WP    G+    C  + F   ++S L   L   WP+L         G
Sbjct: 61  GSDAPTQFTIHGLWPDYNDGSWPSCCYRSDFKEKEISTLMDGLEKYWPSLSCGSPSSCNG 120

Query: 123 NNKWFWGHEWNKHGICSESKF-DQQAYFQMAINM---RNSIDLLGALRVGGLAPNGRNKS 182
               FWGHEW KHG CS   F D+  YF   +N+    N  D+L   + G +A N     
Sbjct: 121 GKGSFWGHEWEKHGTCSSPVFHDEYNYFLTTLNLYLKHNVTDVL--YQAGYVASNSEKYP 180

Query: 183 RQRVQSAIRAQFGKEPVLRCRGTGGQSRLLEIVMCFDDD 195
              + +AI+  F   P + C+    +  + EI +CF  D
Sbjct: 181 LGGIVTAIQNAFHITPEVVCK----RDAIDEIRICFYKD 213

The following BLAST results are available for this feature:

BLAST of Spg015467 vs. NCBI nr


Match Name	E-value	Identity	Description
BAA10891.1	2.5e-115	92.06	ribonuclease (RNase LC1) [Luffa aegyptiaca]	[more]
XP_022142280.1	4.4e-104	81.94	ribonuclease MC-like [Momordica charantia]	[more]
XP_022155091.1	2.3e-100	79.17	ribonuclease MC-like [Momordica charantia]	[more]
XP_008443402.1	4.3e-91	72.22	PREDICTED: ribonuclease MC-like [Cucumis melo]	[more]
XP_038905534.1	5.6e-91	72.22	ribonuclease MC-like [Benincasa hispida]	[more]

BLAST of Spg015467 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P23540	6.7e-63	57.22	Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
P42813	7.7e-35	38.31	Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1	[more]
P80022	2.1e-32	35.47	Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2	[more]
P42815	9.1e-28	34.33	Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1	[more]
P80196	2.1e-24	32.57	Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2	[more]

BLAST of Spg015467 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
Q40115	1.2e-115	92.06	Ribonuclease (RNase LC1) OS=Luffa aegyptiaca OX=3670 PE=2 SV=1	[more]
A0A6J1CKH7	2.1e-104	81.94	Ribonuclease T(2) OS=Momordica charantia OX=3673 GN=LOC111012440 PE=3 SV=1	[more]
A0A6J1DQN7	1.1e-100	79.17	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111022224 PE=3 SV=1	[more]
A0A1S3B8Q3	2.1e-91	72.22	Ribonuclease T(2) OS=Cucumis melo OX=3656 GN=LOC103486996 PE=3 SV=1	[more]
A0A0A0LC37	1.1e-89	72.60	Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G825050 PE=3 SV=1	[more]

BLAST of Spg015467 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G02990.1	5.4e-36	38.31	ribonuclease 1	[more]
AT1G14220.1	3.8e-29	33.80	Ribonuclease T2 family protein	[more]
AT1G26820.1	6.5e-29	34.33	ribonuclease 3	[more]
AT1G14210.1	1.3e-26	32.38	Ribonuclease T2 family protein	[more]
AT2G39780.1	1.7e-13	29.22	ribonuclease 2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 26..212 e-value: 1.6E-53 score: 183.5
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 26..214
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 26..201 e-value: 4.7E-41 score: 141.0
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 14..211
None	No IPR available	PANTHER	PTHR11240:SF49	RIBONUCLEASE 3	coord: 14..211
IPR033130	Ribonuclease T2, His active site 2	PROSITE	PS00531	RNASE_T2_2	coord: 104..115
IPR033697	Ribonuclease T2, eukaryotic	CDD	cd01061	RNase_T2_euk	coord: 26..213 e-value: 7.92804E-79 score: 232.608

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg015467.1	Spg015467.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006401	RNA catabolic process
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding