Spg015154 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg015154
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptioncysteine proteinase RD19a-like
Locationscaffold3: 33766438 .. 33769243 (-)
RNA-Seq ExpressionSpg015154
SyntenySpg015154
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAACATGGATCGGAGTTTCTTCTTCTTCGCCGTTGCCGTCGCCGCCGTCGCCCTCTGCTCATCGGAATCTTTGGCCTCTCTGCATTCCGTCGAACGCGACGGCGATCCGCTGATCCGTCAGGTTGTCGACGATGGCGATTTCAATCGTCTCCGGCTCGGAGCGGAGCACCATTTTTCGTTATTCAAGCGAAAGTTCGGGAAATCGTATGCCACCGAGGAAGAGCACGATCGTAGATTCAAGATCTTCAAGGCTAATATGCGACGGGCGCTACGCCATCAGTCCTTTGATCCGTCCGCCATTCATGGCGTCACTCAGTTCTCCGATTTGACCCCTACCGAGTTCCGCAAGGCCTTTCTAGGGCTCCGAGGTCAGCGTCTCAGGCTTCCTGTTGATGCCAATGAGGCTCCGGTTCTTCCTACAGAGAATCTTCCGACTGATTTTGATTGGAGGGAGCATGGTGCCGTAACTGCTGTGAAAAATCAGGTATCGTGCTATATCTCAACTCAATTCTCCAATCTTTCGTGTAACAGGCGTGATTTCATTCTTGTTCTTCTTTTCTGGAGGATATACCTGTTTGATTTCCTTTAATTTTTGTTTTTAAACAAATTTGAACTTGTGGAAGGTTGAAGGACAATTGTTAATCTTAAATACTTATGCATGTGTTTTCATCCTTGGTTTTTACGGCATATATCAATTCTTTAAAAATGATTTGATTGTAGGATACTGCAACTTCATTACTATTATCTGTTATCTTTGCATTTGGGGTTGTTTAAAAGTTTATAACTAGCAATGAACATCTATGTTGGATCAGGGATCTTGTGGATCTTGCTGGAGTTTCAGTACAACCGGTGCCCTTGAAGGTGCTAACTTCCTTGCGACAGGGGAGCTTGTTAGCTTAAGCGAACAGCAGCTGGTAGATTGTGATCACGAGGTTTGCTCTCTTCTCTGTTTTAAATAGCTATGTAATTGCTCTGTTCTCCTCATTCGCACGTATAAATAGATCATAATAGCTATGAAATAACTTTATTTAAATTTAAGATTTTGATTCATTTGTTGTGAATGTTAGGATGCTTAATCAACTCTTGATGTTTCCTTTGAATTTTCACCTTGCTACTTTCCTTCTTGTCTTATGTTACAGTTAAACATTTCAATCATTAGCTAAAGAATATTCTGATGATGCTAGGTTTGATTATTCTTGTTTTGAATTTTGATAATTATAGCTGCTGATACTTTTTTTAAGAAATTTCATATGGCCTGTGCAAATCAGAAGCGATTATTCATAGGTTGAAGGAGAATCTATGCTTTATGTTTGTTGCCCCTTTAATGATACTATTCTGGAAACCTCTTAACTTGCATTTCTTAATTCTGTGCAGTGTGATCCAGAGGAAGCTGGTTCCTGTGACTCTGGTTGCAAAGGTGGCCTGATGAATAGCGCACTTGAATACACACTAAAAGCCGGAGGTCTGATGAGGGAGCAAGATTATCCCTATACTGGAACTGATAGTGGAACCTGCAAGTTTGACAGGTCCAAGATTGCTGCATCCGTTGCCAATTTCAGCGTTGTTTCACTTGACGAAGATCAAATTGCTGCAAATCTGGTGAAAAATGGCCCACTTGCAAGTAAATACCATCCTATGTCTTTCCTATTCGGATGAAAATTAGGCCCATTTGATAACCGGTTACTTTTTGGTTTTTTGGTTTTTTAAAAAAACACTACTTTCATTCCTGAGTTCTCAAAATCCAAGTCAAGTTTTAAAAAAAAAAAAAAAAATAGTTTGAATTCAAACTTTTATCTAAGGTAAAAACCATAGATGGCGAGGAAACCAACACCATTTTCAAAAACAAAACTAAAAACGAAATTGTTATCGAATGGACCTCTAAGCCCTTTTATCTTCTAATATGGGTCACTAGCGGCAGCTGAACTTTGGTTTTACCTGGATGCAGTTGCTATCAATGCGGTATTCATGCAGACATACATAGGTGGAGTATCTTGTCCATTCATATGTTCAAAGCGGTTGGATCATGGAGTTTTGCTGGTGGGTTATGGCTCAGCTGGCTATGCTCCCATCAGAATGAGAGACAAGGACTACTGGATCATCAAAAACTCTTGGGGACAAAACTGGGGAGAAAATGGCTATTACAAGATCTGCAGAGGAAGGAATGTCTGTGGAGTTGATTCCTTGGTCTCAACTGTTGCGGCAGTTCATATTCCCACTGCAGCAGTATCTGGTCAGTAGATGGGATCTCTCTCAAAAGATTGTAGTTGATGAAACTGTGAATTCTGAGTCTTTAGCTACTCTCATTCGTTGAAGGCAGATGCACATATATATCAGCTACAGCCTATGCATATATGTCAGTCACACTAATAAACCCAACAAAAATAAGAGACCAAGTTGAGTATATATTTTATGATTCATTACTGTTCTTGTGTTGCAAAAATATATGCCAAGAAGCCAAAATTTGATAACAATCATTGGCTCAAAAGCTCTTCGGTTCATGTCCATCCTCAAACAGAATGTAACATTATAAACTAATGAAACAGATGTGGTTCAAATTTCTAAGTTGGAAGAGAATGTCTAACTAGTTGAATTATGTTTAGATCAACAAATTTAGCTCGTTCTCAACAATAATGTTATTTAATACTTATTGTTAGTAGCGATGGTAAGCAACTCTGTGAAATAGTGATACCGTTGAAAATAGGCATGAATGGGGATTTCTTGCTGGTTATCAAAATTTAACTTCTTGCCAAAGGTCTGTAGCAGCAGAATTGTAATGAATCCCAATAATATAAAGTTATAAACTTG

mRNA sequence

ATGGATCGGAGTTTCTTCTTCTTCGCCGTTGCCGTCGCCGCCGTCGCCCTCTGCTCATCGGAATCTTTGGCCTCTCTGCATTCCGTCGAACGCGACGGCGATCCGCTGATCCGTCAGGTTGTCGACGATGGCGATTTCAATCGTCTCCGGCTCGGAGCGGAGCACCATTTTTCGTTATTCAAGCGAAAGTTCGGGAAATCGTATGCCACCGAGGAAGAGCACGATCGTAGATTCAAGATCTTCAAGGCTAATATGCGACGGGCGCTACGCCATCAGTCCTTTGATCCGTCCGCCATTCATGGCGTCACTCAGTTCTCCGATTTGACCCCTACCGAGTTCCGCAAGGCCTTTCTAGGGCTCCGAGGTCAGCGTCTCAGGCTTCCTGTTGATGCCAATGAGGCTCCGGTTCTTCCTACAGAGAATCTTCCGACTGATTTTGATTGGAGGGAGCATGGTGCCGTAACTGCTGTGAAAAATCAGGGATCTTGTGGATCTTGCTGGAGTTTCAGTACAACCGGTGCCCTTGAAGGTGCTAACTTCCTTGCGACAGGGGAGCTTGTTAGCTTAAGCGAACAGCAGCTGGTAGATTGTGATCACGAGTGTGATCCAGAGGAAGCTGGTTCCTGTGACTCTGGTTGCAAAGGTGGCCTGATGAATAGCGCACTTGAATACACACTAAAAGCCGGAGGTCTGATGAGGGAGCAAGATTATCCCTATACTGGAACTGATAGTGGAACCTGCAAGTTTGACAGGTCCAAGATTGCTGCATCCGTTGCCAATTTCAGCGTTGTTTCACTTGACGAAGATCAAATTGCTGCAAATCTGGTGAAAAATGGCCCACTTGCAATTGCTATCAATGCGGTATTCATGCAGACATACATAGGTGGAGTATCTTGTCCATTCATATGTTCAAAGCGGTTGGATCATGGAGTTTTGCTGGTGGGTTATGGCTCAGCTGGCTATGCTCCCATCAGAATGAGAGACAAGGACTACTGGATCATCAAAAACTCTTGGGGACAAAACTGGGGAGAAAATGGCTATTACAAGATCTGCAGAGGAAGGAATGTCTGTGGAGTTGATTCCTTGGTCTCAACTGTTGCGGCAGTTCATATTCCCACTGCAGCAGTATCTGGTCAGTAG

Coding sequence (CDS)

ATGGATCGGAGTTTCTTCTTCTTCGCCGTTGCCGTCGCCGCCGTCGCCCTCTGCTCATCGGAATCTTTGGCCTCTCTGCATTCCGTCGAACGCGACGGCGATCCGCTGATCCGTCAGGTTGTCGACGATGGCGATTTCAATCGTCTCCGGCTCGGAGCGGAGCACCATTTTTCGTTATTCAAGCGAAAGTTCGGGAAATCGTATGCCACCGAGGAAGAGCACGATCGTAGATTCAAGATCTTCAAGGCTAATATGCGACGGGCGCTACGCCATCAGTCCTTTGATCCGTCCGCCATTCATGGCGTCACTCAGTTCTCCGATTTGACCCCTACCGAGTTCCGCAAGGCCTTTCTAGGGCTCCGAGGTCAGCGTCTCAGGCTTCCTGTTGATGCCAATGAGGCTCCGGTTCTTCCTACAGAGAATCTTCCGACTGATTTTGATTGGAGGGAGCATGGTGCCGTAACTGCTGTGAAAAATCAGGGATCTTGTGGATCTTGCTGGAGTTTCAGTACAACCGGTGCCCTTGAAGGTGCTAACTTCCTTGCGACAGGGGAGCTTGTTAGCTTAAGCGAACAGCAGCTGGTAGATTGTGATCACGAGTGTGATCCAGAGGAAGCTGGTTCCTGTGACTCTGGTTGCAAAGGTGGCCTGATGAATAGCGCACTTGAATACACACTAAAAGCCGGAGGTCTGATGAGGGAGCAAGATTATCCCTATACTGGAACTGATAGTGGAACCTGCAAGTTTGACAGGTCCAAGATTGCTGCATCCGTTGCCAATTTCAGCGTTGTTTCACTTGACGAAGATCAAATTGCTGCAAATCTGGTGAAAAATGGCCCACTTGCAATTGCTATCAATGCGGTATTCATGCAGACATACATAGGTGGAGTATCTTGTCCATTCATATGTTCAAAGCGGTTGGATCATGGAGTTTTGCTGGTGGGTTATGGCTCAGCTGGCTATGCTCCCATCAGAATGAGAGACAAGGACTACTGGATCATCAAAAACTCTTGGGGACAAAACTGGGGAGAAAATGGCTATTACAAGATCTGCAGAGGAAGGAATGTCTGTGGAGTTGATTCCTTGGTCTCAACTGTTGCGGCAGTTCATATTCCCACTGCAGCAGTATCTGGTCAGTAG

Protein sequence

MDRSFFFFAVAVAAVALCSSESLASLHSVERDGDPLIRQVVDDGDFNRLRLGAEHHFSLFKRKFGKSYATEEEHDRRFKIFKANMRRALRHQSFDPSAIHGVTQFSDLTPTEFRKAFLGLRGQRLRLPVDANEAPVLPTENLPTDFDWREHGAVTAVKNQGSCGSCWSFSTTGALEGANFLATGELVSLSEQQLVDCDHECDPEEAGSCDSGCKGGLMNSALEYTLKAGGLMREQDYPYTGTDSGTCKFDRSKIAASVANFSVVSLDEDQIAANLVKNGPLAIAINAVFMQTYIGGVSCPFICSKRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGQNWGENGYYKICRGRNVCGVDSLVSTVAAVHIPTAAVSGQ
Homology
BLAST of Spg015154 vs. NCBI nr
Match: XP_038887474.1 (probable cysteine protease RD19B [Benincasa hispida])

HSP 1 Score: 682.9 bits (1761), Expect = 1.5e-192
Identity = 334/379 (88.13%), Postives = 349/379 (92.08%), Query Frame = 0

Query: 1   MDRSFFFFAVAVAAVA-LCSSESLASLHSVERDGDPLIRQVVDDGDF-NRLRLGAEHHFS 60
           MD SF  FAV  AA A LCSSESL S  S+ERDGDPLIRQVVDDG+F NRL LGAEHHFS
Sbjct: 1   MDWSFSLFAVIAAATATLCSSESLTSPRSIERDGDPLIRQVVDDGNFNNRLPLGAEHHFS 60

Query: 61  LFKRKFGKSYATEEEHDRRFKIFKANMRRALRHQSFDPSAIHGVTQFSDLTPTEFRKAFL 120
           LFK++FGKSYATEEEHDRRFKIF+ANMRRA RHQSFDPSAIHG+TQFSDLTP EFRKAFL
Sbjct: 61  LFKQRFGKSYATEEEHDRRFKIFEANMRRAQRHQSFDPSAIHGITQFSDLTPFEFRKAFL 120

Query: 121 GLRGQRLRLPVDANEAPVLPTENLPTDFDWREHGAVTAVKNQGSCGSCWSFSTTGALEGA 180
           GLRG RLRLPVD N AP+LPTENLP DFDWRE GAVT VKNQGSCGSCWSFSTTGALEGA
Sbjct: 121 GLRGHRLRLPVDTNAAPILPTENLPIDFDWRERGAVTPVKNQGSCGSCWSFSTTGALEGA 180

Query: 181 NFLATGELVSLSEQQLVDCDHECDPEEAGSCDSGCKGGLMNSALEYTLKAGGLMREQDYP 240
           NFLATGELVSLSEQQLVDCDHECDPEEA SCDSGC GGLMNSA EYTLKAGGLMRE+DYP
Sbjct: 181 NFLATGELVSLSEQQLVDCDHECDPEEADSCDSGCNGGLMNSAFEYTLKAGGLMREEDYP 240

Query: 241 YTGTDSGTCKFDRSKIAASVANFSVVSLDEDQIAANLVKNGPLAIAINAVFMQTYIGGVS 300
           YTGTD G C FD+SKIAASVANFSVVSLDEDQIAANLVKNGPLAIAINAVFMQTYIGGVS
Sbjct: 241 YTGTDRGNCNFDKSKIAASVANFSVVSLDEDQIAANLVKNGPLAIAINAVFMQTYIGGVS 300

Query: 301 CPFICSKRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGQNWGENGYYKICRGRNVCG 360
           CPFICS+RLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWG+NWGENGYYKICRGRN+CG
Sbjct: 301 CPFICSRRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGENWGENGYYKICRGRNICG 360

Query: 361 VDSLVSTVAAVHIPTAAVS 378
           VDSLVSTVAAVH    +++
Sbjct: 361 VDSLVSTVAAVHTHDTSIA 379

BLAST of Spg015154 vs. NCBI nr
Match: XP_004148554.2 (cysteine protease RD19A [Cucumis sativus] >KGN43159.1 hypothetical protein Csa_020172 [Cucumis sativus])

HSP 1 Score: 671.8 bits (1732), Expect = 3.5e-189
Identity = 328/374 (87.70%), Postives = 342/374 (91.44%), Query Frame = 0

Query: 1   MDRSFFFFAVAVAAVA-LCSSESLASLHSVERDGDPLIRQVVD-DGDFNRLRLGAEHHFS 60
           MDR+FF FAV  A  A LCSSE L S HSVE DGDPLIRQVV+ DGDFN   LGAEHHFS
Sbjct: 1   MDRNFFLFAVITAVTATLCSSEPLVSQHSVEHDGDPLIRQVVENDGDFNHHALGAEHHFS 60

Query: 61  LFKRKFGKSYATEEEHDRRFKIFKANMRRALRHQSFDPSAIHGVTQFSDLTPTEFRKAFL 120
           LFKR+FGKSYATEEEHDRRFKIFKANMRRA RHQSFDPSAIHGVTQFSDLTP EFRKAFL
Sbjct: 61  LFKRRFGKSYATEEEHDRRFKIFKANMRRAERHQSFDPSAIHGVTQFSDLTPFEFRKAFL 120

Query: 121 GLRGQRLRLPVDANEAPVLPTENLPTDFDWREHGAVTAVKNQGSCGSCWSFSTTGALEGA 180
           GLRG RLRLPVD N AP+LPTENLP DFDWR+HG VT VKNQGSCGSCWSFSTTGALEGA
Sbjct: 121 GLRGHRLRLPVDTNAAPILPTENLPIDFDWRQHGGVTRVKNQGSCGSCWSFSTTGALEGA 180

Query: 181 NFLATGELVSLSEQQLVDCDHECDPEEAGSCDSGCKGGLMNSALEYTLKAGGLMREQDYP 240
           NFLATGELVSLSEQQLVDCDHECDPEE  +CDSGC GGLMNSA EYTLKAGGLM+EQDYP
Sbjct: 181 NFLATGELVSLSEQQLVDCDHECDPEEEDACDSGCNGGLMNSAFEYTLKAGGLMKEQDYP 240

Query: 241 YTGTDSGTCKFDRSKIAASVANFSVV-SLDEDQIAANLVKNGPLAIAINAVFMQTYIGGV 300
           Y G D  TC FD+SKIAAS+A+FSVV S+DEDQIAANLVKNGPLAIAINAVFMQTYIGGV
Sbjct: 241 YAGIDRNTCNFDKSKIAASIASFSVVNSIDEDQIAANLVKNGPLAIAINAVFMQTYIGGV 300

Query: 301 SCPFICSKRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGQNWGENGYYKICRGRNVC 360
           SCPFICSKRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWG++WGENGYYKICRGRN+C
Sbjct: 301 SCPFICSKRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGESWGENGYYKICRGRNIC 360

Query: 361 GVDSLVSTVAAVHI 372
           GVDSLVSTVAAVHI
Sbjct: 361 GVDSLVSTVAAVHI 374

BLAST of Spg015154 vs. NCBI nr
Match: XP_008448073.1 (PREDICTED: cysteine proteinase RD19a-like [Cucumis melo])

HSP 1 Score: 670.2 bits (1728), Expect = 1.0e-188
Identity = 327/373 (87.67%), Postives = 341/373 (91.42%), Query Frame = 0

Query: 1   MDRSFFFFAVAVAAVALCSSESLASLHSVERDGDPLIRQVV-DDGDFNRLRLGAEHHFSL 60
           MD +FF FAV + A  LC SESL S HSV+ D DP IRQVV DDGDFNR  LGAEHHFSL
Sbjct: 1   MDLNFFLFAV-ITAATLCLSESLTSPHSVQHDRDPFIRQVVEDDGDFNRHALGAEHHFSL 60

Query: 61  FKRKFGKSYATEEEHDRRFKIFKANMRRALRHQSFDPSAIHGVTQFSDLTPTEFRKAFLG 120
           FKR+FGKSYATEEEHDRRFKIFKANMRRA RHQSFDPSAIHG+TQFSDLTP EFRKAFLG
Sbjct: 61  FKRRFGKSYATEEEHDRRFKIFKANMRRAERHQSFDPSAIHGITQFSDLTPFEFRKAFLG 120

Query: 121 LRGQRLRLPVDANEAPVLPTENLPTDFDWREHGAVTAVKNQGSCGSCWSFSTTGALEGAN 180
           LRG RLRLPVD N AP+LPTENLP DFDWRE GAVT VKNQGSCGSCWSFSTTGA+EGAN
Sbjct: 121 LRGHRLRLPVDTNAAPILPTENLPIDFDWRERGAVTRVKNQGSCGSCWSFSTTGAIEGAN 180

Query: 181 FLATGELVSLSEQQLVDCDHECDPEEAGSCDSGCKGGLMNSALEYTLKAGGLMREQDYPY 240
           FLATG+LVSLSEQQLVDCDHECDPEE  +CDSGC GGLMNSA EYTLKAGGLM+EQDYPY
Sbjct: 181 FLATGKLVSLSEQQLVDCDHECDPEEEDACDSGCNGGLMNSAFEYTLKAGGLMKEQDYPY 240

Query: 241 TGTDSGTCKFDRSKIAASVANFSVV-SLDEDQIAANLVKNGPLAIAINAVFMQTYIGGVS 300
           TGTD  TC FD+SKIAAS+ANFSVV SLDEDQIAANLVKNGPLAIAINAVFMQTYIGGVS
Sbjct: 241 TGTDHSTCNFDKSKIAASIANFSVVNSLDEDQIAANLVKNGPLAIAINAVFMQTYIGGVS 300

Query: 301 CPFICSKRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGQNWGENGYYKICRGRNVCG 360
           CPFICS RLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWG+NWGENGYYKICRGRN+CG
Sbjct: 301 CPFICSNRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGENWGENGYYKICRGRNICG 360

Query: 361 VDSLVSTVAAVHI 372
           VDSLVSTVAAVHI
Sbjct: 361 VDSLVSTVAAVHI 372

BLAST of Spg015154 vs. NCBI nr
Match: XP_022970957.1 (probable cysteine protease RD19B [Cucurbita maxima])

HSP 1 Score: 668.3 bits (1723), Expect = 3.9e-188
Identity = 325/375 (86.67%), Postives = 343/375 (91.47%), Query Frame = 0

Query: 1   MDRSFFFFAVAVAAVALCSSESLASLHSVERDGDPLIRQVVDDGDFNRLRLGAEHHFSLF 60
           MDRSF  F V +AA  LCSSESLAS +SV     PLIRQV+DDG+ N L L AEHHF LF
Sbjct: 1   MDRSFLLFPVVIAAAVLCSSESLASTYSV----SPLIRQVIDDGESNNLPLQAEHHFLLF 60

Query: 61  KRKFGKSYATEEEHDRRFKIFKANMRRALRHQSFDPSAIHGVTQFSDLTPTEFRKAFLGL 120
           KRKFGKSYATEEEH+RRF+IF+ANMRRALRHQSFDPSAIHGVTQFSDLT +EF+K FLGL
Sbjct: 61  KRKFGKSYATEEEHNRRFRIFRANMRRALRHQSFDPSAIHGVTQFSDLTVSEFQKTFLGL 120

Query: 121 RGQRLRLPVDANEAPVLPTENLPTDFDWREHGAVTAVKNQGSCGSCWSFSTTGALEGANF 180
           RG RL+LP+ AN+AP+LPTENLP DFDWRE GAVT VKNQG+CGSCWSFSTTGALEGANF
Sbjct: 121 RGHRLKLPLHANQAPILPTENLPGDFDWRERGAVTPVKNQGTCGSCWSFSTTGALEGANF 180

Query: 181 LATGELVSLSEQQLVDCDHECDPEEAGSCDSGCKGGLMNSALEYTLKAGGLMREQDYPYT 240
           LATGELVSLSEQQLVDCDHECD EEAGSCDSGC GGLMNSALEYTLK GGLMREQDYPYT
Sbjct: 181 LATGELVSLSEQQLVDCDHECDSEEAGSCDSGCNGGLMNSALEYTLKVGGLMREQDYPYT 240

Query: 241 GTDSGTCKFDRSKIAASVANFSVVSLDEDQIAANLVKNGPLAIAINAVFMQTYIGGVSCP 300
           GTD  TCKFDRSKIAASVANFSVVSLDEDQIAANLVKNGPLAIAINAVFMQTYIGGVSCP
Sbjct: 241 GTDRETCKFDRSKIAASVANFSVVSLDEDQIAANLVKNGPLAIAINAVFMQTYIGGVSCP 300

Query: 301 FICSKRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGQNWGENGYYKICRGRNVCGVD 360
           FICSKRLDHGVLLVGYGSAGYAPIRMRD+DYWIIKNSWG NWGENGYY+IC+GRN+CGVD
Sbjct: 301 FICSKRLDHGVLLVGYGSAGYAPIRMRDRDYWIIKNSWGPNWGENGYYRICKGRNICGVD 360

Query: 361 SLVSTVAAVHIPTAA 376
           SLVSTVAAVH P AA
Sbjct: 361 SLVSTVAAVHTPIAA 371

BLAST of Spg015154 vs. NCBI nr
Match: XP_022933443.1 (probable cysteine protease RD19B [Cucurbita moschata])

HSP 1 Score: 662.9 bits (1709), Expect = 1.6e-186
Identity = 325/376 (86.44%), Postives = 344/376 (91.49%), Query Frame = 0

Query: 1   MDRSFFFFAVAV-AAVALCSSESLASLHSVERDGDPLIRQVVDDGDFNRLRLGAEHHFSL 60
           MDRSFF F+V + AA ALCSSESLAS +SV     PLIRQV+DDG+ + L L AEHHF L
Sbjct: 1   MDRSFFLFSVVIAAAAALCSSESLASTYSV----SPLIRQVIDDGESSNLPLQAEHHFLL 60

Query: 61  FKRKFGKSYATEEEHDRRFKIFKANMRRALRHQSFDPSAIHGVTQFSDLTPTEFRKAFLG 120
           FKRKFGKSYATEEEH+RRF+IFKANMRRALRHQSFDPSAIHGVTQFSDLT +EF+K FLG
Sbjct: 61  FKRKFGKSYATEEEHNRRFRIFKANMRRALRHQSFDPSAIHGVTQFSDLTVSEFQKTFLG 120

Query: 121 LRGQRLRLPVDANEAPVLPTENLPTDFDWREHGAVTAVKNQGSCGSCWSFSTTGALEGAN 180
           LRG RL+LP+DAN+AP+LPTENLP  FDWRE GAVT VKNQG+CGSCWSFSTTGALEGAN
Sbjct: 121 LRGHRLKLPLDANQAPILPTENLPGGFDWRERGAVTPVKNQGTCGSCWSFSTTGALEGAN 180

Query: 181 FLATGELVSLSEQQLVDCDHECDPEEAGSCDSGCKGGLMNSALEYTLKAGGLMREQDYPY 240
           FLATGELVSLSEQQLVDCDHECD EEAGSCDSGC GGLMNSALEYTLK GGLMREQDYPY
Sbjct: 181 FLATGELVSLSEQQLVDCDHECDSEEAGSCDSGCNGGLMNSALEYTLKVGGLMREQDYPY 240

Query: 241 TGTDSGTCKFDRSKIAASVANFSVVSLDEDQIAANLVKNGPLAIAINAVFMQTYIGGVSC 300
           TGTD  TCKFDRSKIAASVANFSVVSLDEDQIAANLVKNGPLAIAINAVFMQTYIGGVSC
Sbjct: 241 TGTDRETCKFDRSKIAASVANFSVVSLDEDQIAANLVKNGPLAIAINAVFMQTYIGGVSC 300

Query: 301 PFICSKRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGQNWGENGYYKICRGRNVCGV 360
           PFICSKRLDHGVLLVGYGSAGYAPIRMRD+DYWIIKNSWG NWGENGYY+IC+GRN+CGV
Sbjct: 301 PFICSKRLDHGVLLVGYGSAGYAPIRMRDRDYWIIKNSWGPNWGENGYYRICKGRNICGV 360

Query: 361 DSLVSTVAAVHIPTAA 376
           DSLVSTVAAV  P  A
Sbjct: 361 DSLVSTVAAVQTPIVA 372

BLAST of Spg015154 vs. ExPASy Swiss-Prot
Match: P43296 (Cysteine protease RD19A OS=Arabidopsis thaliana OX=3702 GN=RD19A PE=1 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 1.2e-155
Identity = 272/375 (72.53%), Postives = 308/375 (82.13%), Query Frame = 0

Query: 1   MDRSFFFFAVAVAAVALCSSESLASLHSVERDGDPL-IRQVVDDGDFNRLRLGAEHHFSL 60
           MDR   +F+V V +  + S  S     S   DGD L IRQVV  G      L +E HFSL
Sbjct: 1   MDRLKLYFSVFVLSFFIVSVSS-----SDVNDGDDLVIRQVV--GGAEPQVLTSEDHFSL 60

Query: 61  FKRKFGKSYATEEEHDRRFKIFKANMRRALRHQSFDPSAIHGVTQFSDLTPTEFRKAFLG 120
           FKRKFGK YA+ EEHD RF +FKAN+RRA RHQ  DPSA HGVTQFSDLT +EFRK  LG
Sbjct: 61  FKRKFGKVYASNEEHDYRFSVFKANLRRARRHQKLDPSATHGVTQFSDLTRSEFRKKHLG 120

Query: 121 LRGQRLRLPVDANEAPVLPTENLPTDFDWREHGAVTAVKNQGSCGSCWSFSTTGALEGAN 180
           +R    +LP DAN+AP+LPTENLP DFDWR+HGAVT VKNQGSCGSCWSFS TGALEGAN
Sbjct: 121 VR-SGFKLPKDANKAPILPTENLPEDFDWRDHGAVTPVKNQGSCGSCWSFSATGALEGAN 180

Query: 181 FLATGELVSLSEQQLVDCDHECDPEEAGSCDSGCKGGLMNSALEYTLKAGGLMREQDYPY 240
           FLATG+LVSLSEQQLVDCDHECDPEEA SCDSGC GGLMNSA EYTLK GGLM+E+DYPY
Sbjct: 181 FLATGKLVSLSEQQLVDCDHECDPEEADSCDSGCNGGLMNSAFEYTLKTGGLMKEEDYPY 240

Query: 241 TGTDSGTCKFDRSKIAASVANFSVVSLDEDQIAANLVKNGPLAIAINAVFMQTYIGGVSC 300
           TG D  TCK D+SKI ASV+NFSV+S+DE+QIAANLVKNGPLA+AINA +MQTYIGGVSC
Sbjct: 241 TGKDGKTCKLDKSKIVASVSNFSVISIDEEQIAANLVKNGPLAVAINAGYMQTYIGGVSC 300

Query: 301 PFICSKRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGQNWGENGYYKICRGRNVCGV 360
           P+IC++RL+HGVLLVGYG+AGYAP R ++K YWIIKNSWG+ WGENG+YKIC+GRN+CGV
Sbjct: 301 PYICTRRLNHGVLLVGYGAAGYAPARFKEKPYWIIKNSWGETWGENGFYKICKGRNICGV 360

Query: 361 DSLVSTVAAVHIPTA 375
           DS+VSTVAA    TA
Sbjct: 361 DSMVSTVAATVSTTA 367

BLAST of Spg015154 vs. ExPASy Swiss-Prot
Match: P43295 (Probable cysteine protease RD19B OS=Arabidopsis thaliana OX=3702 GN=RD19B PE=2 SV=2)

HSP 1 Score: 540.8 bits (1392), Expect = 1.2e-152
Identity = 256/341 (75.07%), Postives = 298/341 (87.39%), Query Frame = 0

Query: 28  SVERDGDPLIRQVVDDGDFNRLRLGAEHHFSLFKRKFGKSYATEEEHDRRFKIFKANMRR 87
           SV  D D LIRQVVD+ +     L +E HF+LFK+KFGK Y + EEH  RF +FKAN+ R
Sbjct: 21  SVCGDEDVLIRQVVDETE--PKVLSSEDHFTLFKKKFGKVYGSIEEHYYRFSVFKANLLR 80

Query: 88  ALRHQSFDPSAIHGVTQFSDLTPTEFRKAFLGLRGQRLRLPVDANEAPVLPTENLPTDFD 147
           A+RHQ  DPSA HGVTQFSDLT +EFR+  LG++G   +LP DAN+AP+LPT+NLP +FD
Sbjct: 81  AMRHQKMDPSARHGVTQFSDLTRSEFRRKHLGVKG-GFKLPKDANQAPILPTQNLPEEFD 140

Query: 148 WREHGAVTAVKNQGSCGSCWSFSTTGALEGANFLATGELVSLSEQQLVDCDHECDPEEAG 207
           WR+ GAVT VKNQGSCGSCWSFSTTGALEGA+FLATG+LVSLSEQQLVDCDHECDPEE G
Sbjct: 141 WRDRGAVTPVKNQGSCGSCWSFSTTGALEGAHFLATGKLVSLSEQQLVDCDHECDPEEEG 200

Query: 208 SCDSGCKGGLMNSALEYTLKAGGLMREQDYPYTGTDSGTCKFDRSKIAASVANFSVVSLD 267
           SCDSGC GGLMNSA EYTLK GGLMRE+DYPYTGTD G+CK DRSKI ASV+NFSVVS++
Sbjct: 201 SCDSGCNGGLMNSAFEYTLKTGGLMREKDYPYTGTDGGSCKLDRSKIVASVSNFSVVSIN 260

Query: 268 EDQIAANLVKNGPLAIAINAVFMQTYIGGVSCPFICSKRLDHGVLLVGYGSAGYAPIRMR 327
           EDQIAANL+KNGPLA+AINA +MQTYIGGVSCP+ICS+RL+HGVLLVGYGSAG++  R++
Sbjct: 261 EDQIAANLIKNGPLAVAINAAYMQTYIGGVSCPYICSRRLNHGVLLVGYGSAGFSQARLK 320

Query: 328 DKDYWIIKNSWGQNWGENGYYKICRGRNVCGVDSLVSTVAA 369
           +K YWIIKNSWG++WGENG+YKIC+GRN+CGVDSLVSTVAA
Sbjct: 321 EKPYWIIKNSWGESWGENGFYKICKGRNICGVDSLVSTVAA 358

BLAST of Spg015154 vs. ExPASy Swiss-Prot
Match: Q9SUL1 (Probable cysteine protease RD19C OS=Arabidopsis thaliana OX=3702 GN=RD19C PE=2 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 7.4e-150
Identity = 257/371 (69.27%), Postives = 304/371 (81.94%), Query Frame = 0

Query: 1   MDRSFFFFAVAVAAVALCSSESLASLHSVERDGDPLIRQVVDDGDFNRLRLGAEHHFSLF 60
           MDR  FFF +A   +A     ++ S    +   +P IRQVV + +  +L L AEHHF+LF
Sbjct: 1   MDRVVFFFLIAATLLAGSLGSTVISGEVTDGFVNP-IRQVVPEENDEQL-LNAEHHFTLF 60

Query: 61  KRKFGKSYATEEEHDRRFKIFKANMRRALRHQSFDPSAIHGVTQFSDLTPTEFRKAFLGL 120
           K K+ K+YAT+ EHD RF++FKAN+RRA R+Q  DPSA+HGVTQFSDLTP EFR+ FLGL
Sbjct: 61  KSKYEKTYATQVEHDHRFRVFKANLRRARRNQLLDPSAVHGVTQFSDLTPKEFRRKFLGL 120

Query: 121 RGQRLRLPVDANEAPVLPTENLPTDFDWREHGAVTAVKNQGSCGSCWSFSTTGALEGANF 180
           + +  RLP D   AP+LPT +LPT+FDWRE GAVT VKNQG CGSCWSFS  GALEGA+F
Sbjct: 121 KRRGFRLPTDTQTAPILPTSDLPTEFDWREQGAVTPVKNQGMCGSCWSFSAIGALEGAHF 180

Query: 181 LATGELVSLSEQQLVDCDHECDPEEAGSCDSGCKGGLMNSALEYTLKAGGLMREQDYPYT 240
           LAT ELVSLSEQQLVDCDHECDP +A SCDSGC GGLMN+A EY LKAGGLM+E+DYPYT
Sbjct: 181 LATKELVSLSEQQLVDCDHECDPAQANSCDSGCSGGLMNNAFEYALKAGGLMKEEDYPYT 240

Query: 241 GTDSGTCKFDRSKIAASVANFSVVSLDEDQIAANLVKNGPLAIAINAVFMQTYIGGVSCP 300
           G D   CKFD+SKI ASV+NFSVVS DEDQIAANLV++GPLAIAINA++MQTYIGGVSCP
Sbjct: 241 GRDHTACKFDKSKIVASVSNFSVVSSDEDQIAANLVQHGPLAIAINAMWMQTYIGGVSCP 300

Query: 301 FICSKRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGQNWGENGYYKICRG-RNVCGV 360
           ++CSK  DHGVLLVG+GS+GYAPIR+++K YWIIKNSWG  WGE+GYYKICRG  N+CG+
Sbjct: 301 YVCSKSQDHGVLLVGFGSSGYAPIRLKEKPYWIIKNSWGAMWGEHGYYKICRGPHNMCGM 360

Query: 361 DSLVSTVAAVH 371
           D++VSTVAAVH
Sbjct: 361 DTMVSTVAAVH 369

BLAST of Spg015154 vs. ExPASy Swiss-Prot
Match: P25804 (Cysteine proteinase 15A OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 518.8 bits (1335), Expect = 5.0e-146
Identity = 253/369 (68.56%), Postives = 305/369 (82.66%), Query Frame = 0

Query: 1   MDRSFFFFAVAVAAVALCSSESLASLHSVERDGDPLIRQVVDDGDFNRLRLGAEHHFSLF 60
           MDR F F     AAVA   ++          + D +IRQVVD+ + +   L AEHHF+ F
Sbjct: 1   MDRRFLFALFLFAAVATAVTDD-------TNNDDFIIRQVVDNEEDH--LLNAEHHFTSF 60

Query: 61  KRKFGKSYATEEEHDRRFKIFKANMRRALRHQSFDPSAIHGVTQFSDLTPTEFRKAFLGL 120
           K KF KSYAT+EEHD RF +FK+N+ +A  HQ+ DP+A HG+T+FSDLT +EFR+ FLGL
Sbjct: 61  KSKFSKSYATKEEHDYRFGVFKSNLIKAKLHQNRDPTAEHGITKFSDLTASEFRRQFLGL 120

Query: 121 RGQRLRLPVDANEAPVLPTENLPTDFDWREHGAVTAVKNQGSCGSCWSFSTTGALEGANF 180
           + +RLRLP  A +AP+LPT NLP DFDWRE GAVT VK+QGSCGSCW+FSTTGALEGA++
Sbjct: 121 K-KRLRLPAHAQKAPILPTTNLPEDFDWREKGAVTPVKDQGSCGSCWAFSTTGALEGAHY 180

Query: 181 LATGELVSLSEQQLVDCDHECDPEEAGSCDSGCKGGLMNSALEYTLKAGGLMREQDYPYT 240
           LATG+LVSLSEQQLVDCDH CDPE+AGSCDSGC GGLMN+A EY L++GG+++E+DY YT
Sbjct: 181 LATGKLVSLSEQQLVDCDHVCDPEQAGSCDSGCNGGLMNNAFEYLLESGGVVQEKDYAYT 240

Query: 241 GTDSGTCKFDRSKIAASVANFSVVSLDEDQIAANLVKNGPLAIAINAVFMQTYIGGVSCP 300
           G D G+CKFD+SK+ ASV+NFSVV+LDEDQIAANLVKNGPLA+AINA +MQTY+ GVSCP
Sbjct: 241 GRD-GSCKFDKSKVVASVSNFSVVTLDEDQIAANLVKNGPLAVAINAAWMQTYMSGVSCP 300

Query: 301 FICSK-RLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGQNWGENGYYKICRGRNVCGV 360
           ++C+K RLDHGVLLVG+G   YAPIR+++K YWIIKNSWGQNWGE GYYKICRGRNVCGV
Sbjct: 301 YVCAKSRLDHGVLLVGFGKGAYAPIRLKEKPYWIIKNSWGQNWGEQGYYKICRGRNVCGV 358

Query: 361 DSLVSTVAA 369
           DS+VSTVAA
Sbjct: 361 DSMVSTVAA 358

BLAST of Spg015154 vs. ExPASy Swiss-Prot
Match: Q10716 (Cysteine proteinase 1 OS=Zea mays OX=4577 GN=CCP1 PE=2 SV=1)

HSP 1 Score: 499.6 bits (1285), Expect = 3.1e-140
Identity = 246/368 (66.85%), Postives = 285/368 (77.45%), Query Frame = 0

Query: 10  VAVAAVALCSSESLASLHSVERDGDPLIRQVVDDGDFNRLRLGAEHHFSLFKRKFGKSYA 69
           +A   + L S  S A++ +     DPLIRQVV  GD N L L AE HF  F ++FGKSY 
Sbjct: 1   MAHRVLLLLSLASAAAVAAAVDAEDPLIRQVVPGGDDNDLELNAESHFLSFVQRFGKSYK 60

Query: 70  TEEEHDRRFKIFKANMRRALRHQSFDPSAIHGVTQFSDLTPTEFRKAFLGLRGQR----L 129
             +EH  R  +FK N+RRA RHQ  DPSA HGVT+FSDLTP EFR+ +LGLR  R     
Sbjct: 61  DADEHAYRLSVFKDNLRRARRHQLLDPSAEHGVTKFSDLTPAEFRRTYLGLRKSRRALLR 120

Query: 130 RLPVDANEAPVLPTENLPTDFDWREHGAVTAVKNQGSCGSCWSFSTTGALEGANFLATGE 189
            L   A+EAPVLPT+ LP DFDWR+HGAV  VKNQGSCGSCWSFS +GALEGA++LATG+
Sbjct: 121 ELGESAHEAPVLPTDGLPDDFDWRDHGAVGPVKNQGSCGSCWSFSASGALEGAHYLATGK 180

Query: 190 LVSLSEQQLVDCDHECDPEEAGSCDSGCKGGLMNSALEYTLKAGGLMREQDYPYTGTDSG 249
           L  LSEQQ VDCDHECD  E  SCDSGC GGLM +A  Y  KAGGL  E+DYPYTG+D G
Sbjct: 181 LEVLSEQQFVDCDHECDSSEPDSCDSGCNGGLMTTAFSYLQKAGGLESEKDYPYTGSD-G 240

Query: 250 TCKFDRSKIAASVANFSVVSLDEDQIAANLVKNGPLAIAINAVFMQTYIGGVSCPFICSK 309
            CKFD+SKI ASV NFSVVS+DE QI+ANL+K+GPLAI INA +MQTYIGGVSCP+IC +
Sbjct: 241 KCKFDKSKIVASVQNFSVVSVDEAQISANLIKHGPLAIGINAAYMQTYIGGVSCPYICGR 300

Query: 310 RLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGQNWGENGYYKICRG---RNVCGVDSL 369
            LDHGVLLVGYG++G+APIR++DK YWIIKNSWG+NWGENGYYKICRG   RN CGVDS+
Sbjct: 301 HLDHGVLLVGYGASGFAPIRLKDKPYWIIKNSWGENWGENGYYKICRGSNVRNKCGVDSM 360

Query: 370 VSTVAAVH 371
           VSTV+AVH
Sbjct: 361 VSTVSAVH 367

BLAST of Spg015154 vs. ExPASy TrEMBL
Match: A0A0A0K2B5 (Papain-like cysteine proteinase isoform I OS=Cucumis sativus OX=3659 GN=Csa_7G004120 PE=3 SV=1)

HSP 1 Score: 671.8 bits (1732), Expect = 1.7e-189
Identity = 328/374 (87.70%), Postives = 342/374 (91.44%), Query Frame = 0

Query: 1   MDRSFFFFAVAVAAVA-LCSSESLASLHSVERDGDPLIRQVVD-DGDFNRLRLGAEHHFS 60
           MDR+FF FAV  A  A LCSSE L S HSVE DGDPLIRQVV+ DGDFN   LGAEHHFS
Sbjct: 1   MDRNFFLFAVITAVTATLCSSEPLVSQHSVEHDGDPLIRQVVENDGDFNHHALGAEHHFS 60

Query: 61  LFKRKFGKSYATEEEHDRRFKIFKANMRRALRHQSFDPSAIHGVTQFSDLTPTEFRKAFL 120
           LFKR+FGKSYATEEEHDRRFKIFKANMRRA RHQSFDPSAIHGVTQFSDLTP EFRKAFL
Sbjct: 61  LFKRRFGKSYATEEEHDRRFKIFKANMRRAERHQSFDPSAIHGVTQFSDLTPFEFRKAFL 120

Query: 121 GLRGQRLRLPVDANEAPVLPTENLPTDFDWREHGAVTAVKNQGSCGSCWSFSTTGALEGA 180
           GLRG RLRLPVD N AP+LPTENLP DFDWR+HG VT VKNQGSCGSCWSFSTTGALEGA
Sbjct: 121 GLRGHRLRLPVDTNAAPILPTENLPIDFDWRQHGGVTRVKNQGSCGSCWSFSTTGALEGA 180

Query: 181 NFLATGELVSLSEQQLVDCDHECDPEEAGSCDSGCKGGLMNSALEYTLKAGGLMREQDYP 240
           NFLATGELVSLSEQQLVDCDHECDPEE  +CDSGC GGLMNSA EYTLKAGGLM+EQDYP
Sbjct: 181 NFLATGELVSLSEQQLVDCDHECDPEEEDACDSGCNGGLMNSAFEYTLKAGGLMKEQDYP 240

Query: 241 YTGTDSGTCKFDRSKIAASVANFSVV-SLDEDQIAANLVKNGPLAIAINAVFMQTYIGGV 300
           Y G D  TC FD+SKIAAS+A+FSVV S+DEDQIAANLVKNGPLAIAINAVFMQTYIGGV
Sbjct: 241 YAGIDRNTCNFDKSKIAASIASFSVVNSIDEDQIAANLVKNGPLAIAINAVFMQTYIGGV 300

Query: 301 SCPFICSKRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGQNWGENGYYKICRGRNVC 360
           SCPFICSKRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWG++WGENGYYKICRGRN+C
Sbjct: 301 SCPFICSKRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGESWGENGYYKICRGRNIC 360

Query: 361 GVDSLVSTVAAVHI 372
           GVDSLVSTVAAVHI
Sbjct: 361 GVDSLVSTVAAVHI 374

BLAST of Spg015154 vs. ExPASy TrEMBL
Match: A0A1S3BIU9 (cysteine proteinase RD19a-like OS=Cucumis melo OX=3656 GN=LOC103490359 PE=3 SV=1)

HSP 1 Score: 670.2 bits (1728), Expect = 5.0e-189
Identity = 327/373 (87.67%), Postives = 341/373 (91.42%), Query Frame = 0

Query: 1   MDRSFFFFAVAVAAVALCSSESLASLHSVERDGDPLIRQVV-DDGDFNRLRLGAEHHFSL 60
           MD +FF FAV + A  LC SESL S HSV+ D DP IRQVV DDGDFNR  LGAEHHFSL
Sbjct: 1   MDLNFFLFAV-ITAATLCLSESLTSPHSVQHDRDPFIRQVVEDDGDFNRHALGAEHHFSL 60

Query: 61  FKRKFGKSYATEEEHDRRFKIFKANMRRALRHQSFDPSAIHGVTQFSDLTPTEFRKAFLG 120
           FKR+FGKSYATEEEHDRRFKIFKANMRRA RHQSFDPSAIHG+TQFSDLTP EFRKAFLG
Sbjct: 61  FKRRFGKSYATEEEHDRRFKIFKANMRRAERHQSFDPSAIHGITQFSDLTPFEFRKAFLG 120

Query: 121 LRGQRLRLPVDANEAPVLPTENLPTDFDWREHGAVTAVKNQGSCGSCWSFSTTGALEGAN 180
           LRG RLRLPVD N AP+LPTENLP DFDWRE GAVT VKNQGSCGSCWSFSTTGA+EGAN
Sbjct: 121 LRGHRLRLPVDTNAAPILPTENLPIDFDWRERGAVTRVKNQGSCGSCWSFSTTGAIEGAN 180

Query: 181 FLATGELVSLSEQQLVDCDHECDPEEAGSCDSGCKGGLMNSALEYTLKAGGLMREQDYPY 240
           FLATG+LVSLSEQQLVDCDHECDPEE  +CDSGC GGLMNSA EYTLKAGGLM+EQDYPY
Sbjct: 181 FLATGKLVSLSEQQLVDCDHECDPEEEDACDSGCNGGLMNSAFEYTLKAGGLMKEQDYPY 240

Query: 241 TGTDSGTCKFDRSKIAASVANFSVV-SLDEDQIAANLVKNGPLAIAINAVFMQTYIGGVS 300
           TGTD  TC FD+SKIAAS+ANFSVV SLDEDQIAANLVKNGPLAIAINAVFMQTYIGGVS
Sbjct: 241 TGTDHSTCNFDKSKIAASIANFSVVNSLDEDQIAANLVKNGPLAIAINAVFMQTYIGGVS 300

Query: 301 CPFICSKRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGQNWGENGYYKICRGRNVCG 360
           CPFICS RLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWG+NWGENGYYKICRGRN+CG
Sbjct: 301 CPFICSNRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGENWGENGYYKICRGRNICG 360

Query: 361 VDSLVSTVAAVHI 372
           VDSLVSTVAAVHI
Sbjct: 361 VDSLVSTVAAVHI 372

BLAST of Spg015154 vs. ExPASy TrEMBL
Match: A0A6J1I0L8 (probable cysteine protease RD19B OS=Cucurbita maxima OX=3661 GN=LOC111469766 PE=3 SV=1)

HSP 1 Score: 668.3 bits (1723), Expect = 1.9e-188
Identity = 325/375 (86.67%), Postives = 343/375 (91.47%), Query Frame = 0

Query: 1   MDRSFFFFAVAVAAVALCSSESLASLHSVERDGDPLIRQVVDDGDFNRLRLGAEHHFSLF 60
           MDRSF  F V +AA  LCSSESLAS +SV     PLIRQV+DDG+ N L L AEHHF LF
Sbjct: 1   MDRSFLLFPVVIAAAVLCSSESLASTYSV----SPLIRQVIDDGESNNLPLQAEHHFLLF 60

Query: 61  KRKFGKSYATEEEHDRRFKIFKANMRRALRHQSFDPSAIHGVTQFSDLTPTEFRKAFLGL 120
           KRKFGKSYATEEEH+RRF+IF+ANMRRALRHQSFDPSAIHGVTQFSDLT +EF+K FLGL
Sbjct: 61  KRKFGKSYATEEEHNRRFRIFRANMRRALRHQSFDPSAIHGVTQFSDLTVSEFQKTFLGL 120

Query: 121 RGQRLRLPVDANEAPVLPTENLPTDFDWREHGAVTAVKNQGSCGSCWSFSTTGALEGANF 180
           RG RL+LP+ AN+AP+LPTENLP DFDWRE GAVT VKNQG+CGSCWSFSTTGALEGANF
Sbjct: 121 RGHRLKLPLHANQAPILPTENLPGDFDWRERGAVTPVKNQGTCGSCWSFSTTGALEGANF 180

Query: 181 LATGELVSLSEQQLVDCDHECDPEEAGSCDSGCKGGLMNSALEYTLKAGGLMREQDYPYT 240
           LATGELVSLSEQQLVDCDHECD EEAGSCDSGC GGLMNSALEYTLK GGLMREQDYPYT
Sbjct: 181 LATGELVSLSEQQLVDCDHECDSEEAGSCDSGCNGGLMNSALEYTLKVGGLMREQDYPYT 240

Query: 241 GTDSGTCKFDRSKIAASVANFSVVSLDEDQIAANLVKNGPLAIAINAVFMQTYIGGVSCP 300
           GTD  TCKFDRSKIAASVANFSVVSLDEDQIAANLVKNGPLAIAINAVFMQTYIGGVSCP
Sbjct: 241 GTDRETCKFDRSKIAASVANFSVVSLDEDQIAANLVKNGPLAIAINAVFMQTYIGGVSCP 300

Query: 301 FICSKRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGQNWGENGYYKICRGRNVCGVD 360
           FICSKRLDHGVLLVGYGSAGYAPIRMRD+DYWIIKNSWG NWGENGYY+IC+GRN+CGVD
Sbjct: 301 FICSKRLDHGVLLVGYGSAGYAPIRMRDRDYWIIKNSWGPNWGENGYYRICKGRNICGVD 360

Query: 361 SLVSTVAAVHIPTAA 376
           SLVSTVAAVH P AA
Sbjct: 361 SLVSTVAAVHTPIAA 371

BLAST of Spg015154 vs. ExPASy TrEMBL
Match: A0A6J1F4R7 (probable cysteine protease RD19B OS=Cucurbita moschata OX=3662 GN=LOC111440857 PE=3 SV=1)

HSP 1 Score: 662.9 bits (1709), Expect = 7.9e-187
Identity = 325/376 (86.44%), Postives = 344/376 (91.49%), Query Frame = 0

Query: 1   MDRSFFFFAVAV-AAVALCSSESLASLHSVERDGDPLIRQVVDDGDFNRLRLGAEHHFSL 60
           MDRSFF F+V + AA ALCSSESLAS +SV     PLIRQV+DDG+ + L L AEHHF L
Sbjct: 1   MDRSFFLFSVVIAAAAALCSSESLASTYSV----SPLIRQVIDDGESSNLPLQAEHHFLL 60

Query: 61  FKRKFGKSYATEEEHDRRFKIFKANMRRALRHQSFDPSAIHGVTQFSDLTPTEFRKAFLG 120
           FKRKFGKSYATEEEH+RRF+IFKANMRRALRHQSFDPSAIHGVTQFSDLT +EF+K FLG
Sbjct: 61  FKRKFGKSYATEEEHNRRFRIFKANMRRALRHQSFDPSAIHGVTQFSDLTVSEFQKTFLG 120

Query: 121 LRGQRLRLPVDANEAPVLPTENLPTDFDWREHGAVTAVKNQGSCGSCWSFSTTGALEGAN 180
           LRG RL+LP+DAN+AP+LPTENLP  FDWRE GAVT VKNQG+CGSCWSFSTTGALEGAN
Sbjct: 121 LRGHRLKLPLDANQAPILPTENLPGGFDWRERGAVTPVKNQGTCGSCWSFSTTGALEGAN 180

Query: 181 FLATGELVSLSEQQLVDCDHECDPEEAGSCDSGCKGGLMNSALEYTLKAGGLMREQDYPY 240
           FLATGELVSLSEQQLVDCDHECD EEAGSCDSGC GGLMNSALEYTLK GGLMREQDYPY
Sbjct: 181 FLATGELVSLSEQQLVDCDHECDSEEAGSCDSGCNGGLMNSALEYTLKVGGLMREQDYPY 240

Query: 241 TGTDSGTCKFDRSKIAASVANFSVVSLDEDQIAANLVKNGPLAIAINAVFMQTYIGGVSC 300
           TGTD  TCKFDRSKIAASVANFSVVSLDEDQIAANLVKNGPLAIAINAVFMQTYIGGVSC
Sbjct: 241 TGTDRETCKFDRSKIAASVANFSVVSLDEDQIAANLVKNGPLAIAINAVFMQTYIGGVSC 300

Query: 301 PFICSKRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGQNWGENGYYKICRGRNVCGV 360
           PFICSKRLDHGVLLVGYGSAGYAPIRMRD+DYWIIKNSWG NWGENGYY+IC+GRN+CGV
Sbjct: 301 PFICSKRLDHGVLLVGYGSAGYAPIRMRDRDYWIIKNSWGPNWGENGYYRICKGRNICGV 360

Query: 361 DSLVSTVAAVHIPTAA 376
           DSLVSTVAAV  P  A
Sbjct: 361 DSLVSTVAAVQTPIVA 372

BLAST of Spg015154 vs. ExPASy TrEMBL
Match: A0A5A7SS65 (Cysteine proteinase RD19a-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold142G00480 PE=3 SV=1)

HSP 1 Score: 652.1 bits (1681), Expect = 1.4e-183
Identity = 322/373 (86.33%), Postives = 336/373 (90.08%), Query Frame = 0

Query: 1   MDRSFFFFAVAVAAVALCSSESLASLHSVERDGDPLIRQVV-DDGDFNRLRLGAEHHFSL 60
           MD +FF FAV + A  LC SESL S HSV+ D DP IRQVV DD DFNR  LGAEHHFSL
Sbjct: 1   MDLNFFLFAV-ITAATLCLSESLTSPHSVQHDRDPFIRQVVEDDSDFNRHALGAEHHFSL 60

Query: 61  FKRKFGKSYATEEEHDRRFKIFKANMRRALRHQSFDPSAIHGVTQFSDLTPTEFRKAFLG 120
           FKR+FGKSYATEEEHDRRFKIFKANMRRA RHQSFDPSAIHG+TQFSDLTP EFRKAFLG
Sbjct: 61  FKRRFGKSYATEEEHDRRFKIFKANMRRAERHQSFDPSAIHGITQFSDLTPFEFRKAFLG 120

Query: 121 LRGQRLRLPVDANEAPVLPTENLPTDFDWREHGAVTAVKNQGSCGSCWSFSTTGALEGAN 180
           LRG RLRLPVD N AP+LPTENLP DFDWRE GAVT VKNQGSCGSCWSFSTTGA+EGAN
Sbjct: 121 LRGHRLRLPVDTNAAPILPTENLPIDFDWRERGAVTRVKNQGSCGSCWSFSTTGAIEGAN 180

Query: 181 FLATGELVSLSEQQLVDCDHECDPEEAGSCDSGCKGGLMNSALEYTLKAGGLMREQDYPY 240
           FLATG+LVSLSEQQLVDCDH    EE  +CDSGC GGLMNSA EYTLKAGGLM+EQDYPY
Sbjct: 181 FLATGKLVSLSEQQLVDCDH----EEEDACDSGCNGGLMNSAFEYTLKAGGLMKEQDYPY 240

Query: 241 TGTDSGTCKFDRSKIAASVANFSVV-SLDEDQIAANLVKNGPLAIAINAVFMQTYIGGVS 300
           TGTD  TC FD+SKIAAS+ANFSVV SLDEDQIAANLVKNGPLAIAINAVFMQTYIGGVS
Sbjct: 241 TGTDHSTCNFDKSKIAASIANFSVVNSLDEDQIAANLVKNGPLAIAINAVFMQTYIGGVS 300

Query: 301 CPFICSKRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGQNWGENGYYKICRGRNVCG 360
           CPFICS RLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWG+NWGENGYYKICRGRN+CG
Sbjct: 301 CPFICSNRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGENWGENGYYKICRGRNICG 360

Query: 361 VDSLVSTVAAVHI 372
           VDSLVSTVAAVHI
Sbjct: 361 VDSLVSTVAAVHI 368

BLAST of Spg015154 vs. TAIR 10
Match: AT4G39090.1 (Papain family cysteine protease )

HSP 1 Score: 550.8 bits (1418), Expect = 8.4e-157
Identity = 272/375 (72.53%), Postives = 308/375 (82.13%), Query Frame = 0

Query: 1   MDRSFFFFAVAVAAVALCSSESLASLHSVERDGDPL-IRQVVDDGDFNRLRLGAEHHFSL 60
           MDR   +F+V V +  + S  S     S   DGD L IRQVV  G      L +E HFSL
Sbjct: 1   MDRLKLYFSVFVLSFFIVSVSS-----SDVNDGDDLVIRQVV--GGAEPQVLTSEDHFSL 60

Query: 61  FKRKFGKSYATEEEHDRRFKIFKANMRRALRHQSFDPSAIHGVTQFSDLTPTEFRKAFLG 120
           FKRKFGK YA+ EEHD RF +FKAN+RRA RHQ  DPSA HGVTQFSDLT +EFRK  LG
Sbjct: 61  FKRKFGKVYASNEEHDYRFSVFKANLRRARRHQKLDPSATHGVTQFSDLTRSEFRKKHLG 120

Query: 121 LRGQRLRLPVDANEAPVLPTENLPTDFDWREHGAVTAVKNQGSCGSCWSFSTTGALEGAN 180
           +R    +LP DAN+AP+LPTENLP DFDWR+HGAVT VKNQGSCGSCWSFS TGALEGAN
Sbjct: 121 VR-SGFKLPKDANKAPILPTENLPEDFDWRDHGAVTPVKNQGSCGSCWSFSATGALEGAN 180

Query: 181 FLATGELVSLSEQQLVDCDHECDPEEAGSCDSGCKGGLMNSALEYTLKAGGLMREQDYPY 240
           FLATG+LVSLSEQQLVDCDHECDPEEA SCDSGC GGLMNSA EYTLK GGLM+E+DYPY
Sbjct: 181 FLATGKLVSLSEQQLVDCDHECDPEEADSCDSGCNGGLMNSAFEYTLKTGGLMKEEDYPY 240

Query: 241 TGTDSGTCKFDRSKIAASVANFSVVSLDEDQIAANLVKNGPLAIAINAVFMQTYIGGVSC 300
           TG D  TCK D+SKI ASV+NFSV+S+DE+QIAANLVKNGPLA+AINA +MQTYIGGVSC
Sbjct: 241 TGKDGKTCKLDKSKIVASVSNFSVISIDEEQIAANLVKNGPLAVAINAGYMQTYIGGVSC 300

Query: 301 PFICSKRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGQNWGENGYYKICRGRNVCGV 360
           P+IC++RL+HGVLLVGYG+AGYAP R ++K YWIIKNSWG+ WGENG+YKIC+GRN+CGV
Sbjct: 301 PYICTRRLNHGVLLVGYGAAGYAPARFKEKPYWIIKNSWGETWGENGFYKICKGRNICGV 360

Query: 361 DSLVSTVAAVHIPTA 375
           DS+VSTVAA    TA
Sbjct: 361 DSMVSTVAATVSTTA 367

BLAST of Spg015154 vs. TAIR 10
Match: AT2G21430.1 (Papain family cysteine protease )

HSP 1 Score: 540.8 bits (1392), Expect = 8.7e-154
Identity = 256/341 (75.07%), Postives = 298/341 (87.39%), Query Frame = 0

Query: 28  SVERDGDPLIRQVVDDGDFNRLRLGAEHHFSLFKRKFGKSYATEEEHDRRFKIFKANMRR 87
           SV  D D LIRQVVD+ +     L +E HF+LFK+KFGK Y + EEH  RF +FKAN+ R
Sbjct: 21  SVCGDEDVLIRQVVDETE--PKVLSSEDHFTLFKKKFGKVYGSIEEHYYRFSVFKANLLR 80

Query: 88  ALRHQSFDPSAIHGVTQFSDLTPTEFRKAFLGLRGQRLRLPVDANEAPVLPTENLPTDFD 147
           A+RHQ  DPSA HGVTQFSDLT +EFR+  LG++G   +LP DAN+AP+LPT+NLP +FD
Sbjct: 81  AMRHQKMDPSARHGVTQFSDLTRSEFRRKHLGVKG-GFKLPKDANQAPILPTQNLPEEFD 140

Query: 148 WREHGAVTAVKNQGSCGSCWSFSTTGALEGANFLATGELVSLSEQQLVDCDHECDPEEAG 207
           WR+ GAVT VKNQGSCGSCWSFSTTGALEGA+FLATG+LVSLSEQQLVDCDHECDPEE G
Sbjct: 141 WRDRGAVTPVKNQGSCGSCWSFSTTGALEGAHFLATGKLVSLSEQQLVDCDHECDPEEEG 200

Query: 208 SCDSGCKGGLMNSALEYTLKAGGLMREQDYPYTGTDSGTCKFDRSKIAASVANFSVVSLD 267
           SCDSGC GGLMNSA EYTLK GGLMRE+DYPYTGTD G+CK DRSKI ASV+NFSVVS++
Sbjct: 201 SCDSGCNGGLMNSAFEYTLKTGGLMREKDYPYTGTDGGSCKLDRSKIVASVSNFSVVSIN 260

Query: 268 EDQIAANLVKNGPLAIAINAVFMQTYIGGVSCPFICSKRLDHGVLLVGYGSAGYAPIRMR 327
           EDQIAANL+KNGPLA+AINA +MQTYIGGVSCP+ICS+RL+HGVLLVGYGSAG++  R++
Sbjct: 261 EDQIAANLIKNGPLAVAINAAYMQTYIGGVSCPYICSRRLNHGVLLVGYGSAGFSQARLK 320

Query: 328 DKDYWIIKNSWGQNWGENGYYKICRGRNVCGVDSLVSTVAA 369
           +K YWIIKNSWG++WGENG+YKIC+GRN+CGVDSLVSTVAA
Sbjct: 321 EKPYWIIKNSWGESWGENGFYKICKGRNICGVDSLVSTVAA 358

BLAST of Spg015154 vs. TAIR 10
Match: AT4G16190.1 (Papain family cysteine protease )

HSP 1 Score: 531.6 bits (1368), Expect = 5.3e-151
Identity = 257/371 (69.27%), Postives = 304/371 (81.94%), Query Frame = 0

Query: 1   MDRSFFFFAVAVAAVALCSSESLASLHSVERDGDPLIRQVVDDGDFNRLRLGAEHHFSLF 60
           MDR  FFF +A   +A     ++ S    +   +P IRQVV + +  +L L AEHHF+LF
Sbjct: 1   MDRVVFFFLIAATLLAGSLGSTVISGEVTDGFVNP-IRQVVPEENDEQL-LNAEHHFTLF 60

Query: 61  KRKFGKSYATEEEHDRRFKIFKANMRRALRHQSFDPSAIHGVTQFSDLTPTEFRKAFLGL 120
           K K+ K+YAT+ EHD RF++FKAN+RRA R+Q  DPSA+HGVTQFSDLTP EFR+ FLGL
Sbjct: 61  KSKYEKTYATQVEHDHRFRVFKANLRRARRNQLLDPSAVHGVTQFSDLTPKEFRRKFLGL 120

Query: 121 RGQRLRLPVDANEAPVLPTENLPTDFDWREHGAVTAVKNQGSCGSCWSFSTTGALEGANF 180
           + +  RLP D   AP+LPT +LPT+FDWRE GAVT VKNQG CGSCWSFS  GALEGA+F
Sbjct: 121 KRRGFRLPTDTQTAPILPTSDLPTEFDWREQGAVTPVKNQGMCGSCWSFSAIGALEGAHF 180

Query: 181 LATGELVSLSEQQLVDCDHECDPEEAGSCDSGCKGGLMNSALEYTLKAGGLMREQDYPYT 240
           LAT ELVSLSEQQLVDCDHECDP +A SCDSGC GGLMN+A EY LKAGGLM+E+DYPYT
Sbjct: 181 LATKELVSLSEQQLVDCDHECDPAQANSCDSGCSGGLMNNAFEYALKAGGLMKEEDYPYT 240

Query: 241 GTDSGTCKFDRSKIAASVANFSVVSLDEDQIAANLVKNGPLAIAINAVFMQTYIGGVSCP 300
           G D   CKFD+SKI ASV+NFSVVS DEDQIAANLV++GPLAIAINA++MQTYIGGVSCP
Sbjct: 241 GRDHTACKFDKSKIVASVSNFSVVSSDEDQIAANLVQHGPLAIAINAMWMQTYIGGVSCP 300

Query: 301 FICSKRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGQNWGENGYYKICRG-RNVCGV 360
           ++CSK  DHGVLLVG+GS+GYAPIR+++K YWIIKNSWG  WGE+GYYKICRG  N+CG+
Sbjct: 301 YVCSKSQDHGVLLVGFGSSGYAPIRLKEKPYWIIKNSWGAMWGEHGYYKICRGPHNMCGM 360

Query: 361 DSLVSTVAAVH 371
           D++VSTVAAVH
Sbjct: 361 DTMVSTVAAVH 369

BLAST of Spg015154 vs. TAIR 10
Match: AT3G54940.2 (Papain family cysteine protease )

HSP 1 Score: 412.9 bits (1060), Expect = 2.7e-115
Identity = 203/347 (58.50%), Postives = 254/347 (73.20%), Query Frame = 0

Query: 27  HSVERDGDPLIRQVVDDGDF---NRLRLGAEHHFSLFKRKFGKSYATEEEHDRRFKIFKA 86
           H V    D  IRQV  D      N L    E  F LF   +GK+Y+T EE+  R  IF  
Sbjct: 18  HVVASVEDLTIRQVTADNRRIRPNLLGTHTESKFRLFMSDYGKNYSTREEYIHRLGIFAK 77

Query: 87  NMRRALRHQSFDPSAIHGVTQFSDLTPTEFRKAFLGLR--GQRLRLPVDANEAPVLPTEN 146
           N+ +A  HQ  DPSA+HGVTQFSDLT  EF++ + G+   G      V A EAP++  + 
Sbjct: 78  NVLKAAEHQMMDPSAVHGVTQFSDLTEEEFKRMYTGVADVGGSRGGTVGA-EAPMVEVDG 137

Query: 147 LPTDFDWREHGAVTAVKNQGSCGSCWSFSTTGALEGANFLATGELVSLSEQQLVDCDHEC 206
           LP DFDWRE G VT VKNQG+CGSCW+FSTTGA EGA+F++TG+L+SLSEQQLVDCD  C
Sbjct: 138 LPEDFDWREKGGVTEVKNQGACGSCWAFSTTGAAEGAHFVSTGKLLSLSEQQLVDCDQAC 197

Query: 207 DPEEAGSCDSGCKGGLMNSALEYTLKAGGLMREQDYPYTGTDSGTCKFDRSKIAASVANF 266
           DP++  +CD+GC GGLM +A EY ++AGGL  E+ YPYTG   G CKFD  K+A  V NF
Sbjct: 198 DPKDKKACDNGCGGGLMTNAYEYLMEAGGLEEERSYPYTG-KRGHCKFDPEKVAVRVLNF 257

Query: 267 SVVSLDEDQIAANLVKNGPLAIAINAVFMQTYIGGVSCPFICSKR-LDHGVLLVGYGSAG 326
           + + LDE+QIAANLV++GPLA+ +NAVFMQTYIGGVSCP ICSKR ++HGVLLVGYGS G
Sbjct: 258 TTIPLDENQIAANLVRHGPLAVGLNAVFMQTYIGGVSCPLICSKRNVNHGVLLVGYGSKG 317

Query: 327 YAPIRMRDKDYWIIKNSWGQNWGENGYYKICRGRNVCGVDSLVSTVA 368
           ++ +R+ +K YWIIKNSWG+ WGENGYYK+CRG ++CG++S+VS VA
Sbjct: 318 FSILRLSNKPYWIIKNSWGKKWGENGYYKLCRGHDICGINSMVSAVA 362

BLAST of Spg015154 vs. TAIR 10
Match: AT3G19390.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 227.3 bits (578), Expect = 2.1e-59
Identity = 126/307 (41.04%), Postives = 175/307 (57.00%), Query Frame = 0

Query: 66  KSYATEEEHDRRFKIFKANMRRALRHQSFDPSAIH-GVTQFSDLTPTEFRKAFLGLRGQR 125
           K+Y    E +RRF+IFK N++    H S        G+T+F+DLT  EFR  +L  + +R
Sbjct: 52  KNYNGLGEKERRFEIFKDNLKFVEEHSSIPNRTYEVGLTRFADLTNDEFRAIYLRSKMER 111

Query: 126 LRLPVDANEAPVLPTENLPTDFDWREHGAVTAVKNQGSCGSCWSFSTTGALEGANFLATG 185
            R+PV   +      ++LP   DWR  GAV  VK+QGSCGSCW+FS  GA+EG N + TG
Sbjct: 112 TRVPVKGEKYLYKVGDSLPDAIDWRAKGAVNPVKDQGSCGSCWAFSAIGAVEGINQIKTG 171

Query: 186 ELVSLSEQQLVDCDHECDPEEAGSCDSGCKGGLMNSALEYTLKAGGLMREQDYPYTGTDS 245
           EL+SLSEQ+LVDCD         S + GC GGLM+ A ++ ++ GG+  E+DYPY  TD 
Sbjct: 172 ELISLSEQELVDCD--------TSYNDGCGGGLMDYAFKFIIENGGIDTEEDYPYIATDV 231

Query: 246 GTCKFDRSKI-AASVANFSVVSLDEDQIAANLVKNGPLAIAINA--VFMQTYIGGVSCPF 305
             C  D+      ++  +  V  ++++     + N P+++AI A     Q Y  GV    
Sbjct: 232 NVCNSDKKNTRVVTIDGYEDVPQNDEKSLKKALANQPISVAIEAGGRAFQLYTSGVFTG- 291

Query: 306 ICSKRLDHGVLLVGYGSAGYAPIRMRDKDYWIIKNSWGQNWGENGYYKICR----GRNVC 365
            C   LDHGV+ VGYGS G        +DYWI++NSWG NWGE+GY+K+ R        C
Sbjct: 292 TCGTSLDHGVVAVGYGSEG-------GQDYWIVRNSWGSNWGESGYFKLERNIKESSGKC 342

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038887474.11.5e-19288.13probable cysteine protease RD19B [Benincasa hispida][more]
XP_004148554.23.5e-18987.70cysteine protease RD19A [Cucumis sativus] >KGN43159.1 hypothetical protein Csa_0... [more]
XP_008448073.11.0e-18887.67PREDICTED: cysteine proteinase RD19a-like [Cucumis melo][more]
XP_022970957.13.9e-18886.67probable cysteine protease RD19B [Cucurbita maxima][more]
XP_022933443.11.6e-18686.44probable cysteine protease RD19B [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
P432961.2e-15572.53Cysteine protease RD19A OS=Arabidopsis thaliana OX=3702 GN=RD19A PE=1 SV=1[more]
P432951.2e-15275.07Probable cysteine protease RD19B OS=Arabidopsis thaliana OX=3702 GN=RD19B PE=2 S... [more]
Q9SUL17.4e-15069.27Probable cysteine protease RD19C OS=Arabidopsis thaliana OX=3702 GN=RD19C PE=2 S... [more]
P258045.0e-14668.56Cysteine proteinase 15A OS=Pisum sativum OX=3888 PE=2 SV=1[more]
Q107163.1e-14066.85Cysteine proteinase 1 OS=Zea mays OX=4577 GN=CCP1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0K2B51.7e-18987.70Papain-like cysteine proteinase isoform I OS=Cucumis sativus OX=3659 GN=Csa_7G00... [more]
A0A1S3BIU95.0e-18987.67cysteine proteinase RD19a-like OS=Cucumis melo OX=3656 GN=LOC103490359 PE=3 SV=1[more]
A0A6J1I0L81.9e-18886.67probable cysteine protease RD19B OS=Cucurbita maxima OX=3661 GN=LOC111469766 PE=... [more]
A0A6J1F4R77.9e-18786.44probable cysteine protease RD19B OS=Cucurbita moschata OX=3662 GN=LOC111440857 P... [more]
A0A5A7SS651.4e-18386.33Cysteine proteinase RD19a-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s... [more]
Match NameE-valueIdentityDescription
AT4G39090.18.4e-15772.53Papain family cysteine protease [more]
AT2G21430.18.7e-15475.07Papain family cysteine protease [more]
AT4G16190.15.3e-15169.27Papain family cysteine protease [more]
AT3G54940.22.7e-11558.50Papain family cysteine protease [more]
AT3G19390.12.1e-5941.04Granulin repeat cysteine protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000668Peptidase C1A, papain C-terminalPRINTSPR00705PAPAINcoord: 331..337
score: 75.14
coord: 160..175
score: 64.12
coord: 309..319
score: 58.8
IPR000668Peptidase C1A, papain C-terminalSMARTSM00645pept_c1coord: 142..367
e-value: 1.7E-107
score: 373.1
IPR000668Peptidase C1A, papain C-terminalPFAMPF00112Peptidase_C1coord: 142..365
e-value: 6.3E-73
score: 245.5
IPR013201Cathepsin propeptide inhibitor domain (I29)SMARTSM00848Inhibitor_I29_2coord: 57..113
e-value: 9.8E-21
score: 84.9
IPR013201Cathepsin propeptide inhibitor domain (I29)PFAMPF08246Inhibitor_I29coord: 57..113
e-value: 4.4E-11
score: 43.1
NoneNo IPR availableGENE3D3.90.70.10Cysteine proteinasescoord: 27..367
e-value: 5.0E-96
score: 324.2
NoneNo IPR availablePANTHERPTHR12411CYSTEINE PROTEASE FAMILY C1-RELATEDcoord: 30..368
NoneNo IPR availablePANTHERPTHR12411:SF783CYSTEINE PROTEASE RD19C-RELATEDcoord: 30..368
IPR025660Cysteine peptidase, histidine active sitePROSITEPS00639THIOL_PROTEASE_HIScoord: 307..317
IPR000169Cysteine peptidase, cysteine active sitePROSITEPS00139THIOL_PROTEASE_CYScoord: 160..171
IPR025661Cysteine peptidase, asparagine active sitePROSITEPS00640THIOL_PROTEASE_ASNcoord: 331..350
IPR039417Papain-like cysteine endopeptidaseCDDcd02248Peptidase_C1Acoord: 143..366
e-value: 1.63203E-103
score: 302.236
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 55..366

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg015154.1Spg015154.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051603 proteolysis involved in cellular protein catabolic process
biological_process GO:0006508 proteolysis
cellular_component GO:0005615 extracellular space
cellular_component GO:0005764 lysosome
molecular_function GO:0004197 cysteine-type endopeptidase activity
molecular_function GO:0008234 cysteine-type peptidase activity