Spg015131 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg015131
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionRop guanine nucleotide exchange factor 12-like
Locationscaffold3: 48174740 .. 48178240 (+)
RNA-Seq ExpressionSpg015131
SyntenySpg015131
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAAAGAGGAAATGACACTGACTCAAATGATGATCAAGCTTCAAAATAACACCAAGAACTAATTTTAAGATTTAGTGAAAAATATAAAAAACCAAATTTGGACACGGGAAATGACACAAGCTCAAATGATGATCAAGTCGATTCCATGATTATAAGTTCATTTCATATTAGATCAAGCCAGAATAAGACTATGTTTATACATTCTAATTTTGAGAAGGAATCATTCGAGAGTGTGTACATCCAAACAAAATTAAAAAAATAATAATAATATATGCCCCTCCATGAGTGACTTTAACCTAATTTAAAAAATTAATAATGTTCCTTCATGAGTGACAGTACACACAAATACAAATTAAGAAAAGGCAAGAAAATGGAATTGAGCCATTTTGAGACAAAAAAAAAATCACTGGTCTTCTTCTTCCTGCAGTTAAAATGGCTGTCCATTATACAGAGAGAGAAAGAAATAGTTATGAATGAAGGATGGCTGTGTTTCCATTTTTTTTAGCAAAGTTTGACCTGAAGATTTTACACCTGAACCATCCAATTGAAGAACTCTGAAGAAAAAAAGAAAACAAAACCAAAACTTTCCTTATTCCTCAGTTCAAAGAAGCAAAACAAAAATATCCCAACACCCTTTTCTTTTTCTCCTCAAATTCCTTCTTCTTCTTCTCAATTCTCTTCATCTTCTTCTCTCTATGCTGACTGAAACCCACAAGGCTTTCATTCCATCACCCAGTTGCCGATTTGTTGCCCCTTTTGTTTTCAGTGGACCGTTTGATCAAGAAACTGATGATGGTTCGTGCGATGGAGAGCGTGGAGCACGAGGGTTTCAAGTCCAGATTCTTCCATTTCAGAGGAAACCAAGAGAATGCTGGCAGACATGCCAAGAGCTTGAGCATGGAGAGCGCCAGCATGTTCGATTCTCTCCTCGAAGAAGATCTCTCGTCGAGAAGCCAAGGATCGACGCCGGTTCACAACAACTCCGCCGAGAAACAGCAATCTGGGCTGCCGCTGGCGCCATCGAATCGACCTGAAAAGGCTCTGAAGGTGAAGTTGAGCAGCAGGGACGACGACCCTGCCACTGAGAAGGAAGCCAGAGACAAGATAGCCTCAGGTCCGTAAATTCGTTTGTTAAAGTTCAAAACTACACATTGTTCATTTGTTAAAAGTTAAGAACTAAAATGGTTGTTTTCAAGACTAAAATAGGCATATTAGAGGTTAAGGGGTTAACCCAGAACAAACTCAAAAGAACTAAAATAGGCATGTGGAGGAGATTAGGTATGAAAAGGAGGGTGTTATTTATTGTAAAGAGAAAGGGGTTTTTACTTGATTCTGCCATTAATTTTGCCTTTGCAATTGGATTATAATTCAGAAATGGAACAGATCAAAGAGAGGTTTGCAAAGTTACTGTTGGGGGAAGATATGTCTGGGGGAGGGAAGGGTGTTTCCTCAGCCTTGGCATTGTCTAATGCCATCACAAACCTTGCTGGTATGTGCCTAGAATTCTCTAACATAGCTAAATTTACCGTAACCCATCGGCTTAAGCTTTGTTGGGATGTAAAATTCTGCGAATTTTGCAGCTTCTGTGTTTGGAGAACAATGGCGACTCGAGCCAATGTCAGAAGAAAGAAAAGCAAGATGGAGAAAAGAAATCGATTTGCTCTTATCTGTCACGGATTACATTGTTGAGTTTGTTCCTTCACAGCAGAAATCCAAGGATGGAACAAATATGGAGGTAAACTTAAACATCCAATTTGAATCTGCTGTACTGATATGATTTCTTGGCTTGGCCCAAATATTCCTTCCTTTTTGTTGTAGATAATGGTGACTCGGCAACGAAATGATTTGCACTTGAATATCCCAGCTTTGAGGAAGCTTGATGCCATGTTAATTGTAAGAACTTTTTTATGCCTTAAGTTTACTGTATTTTTGCTGGTTCAGTCAAACGTGATACTGGATTTGTCACGACAAAACACAGGATTGTCTGGACAATTTTAAAGACCAAAATGAGTTCTACTATGTATCGAGAGACGCCGATGACTCGGAGAAAGGAAATAACAAGAGGAAAGATGATAAATGGTGGCTTCCTACAGCCAAAGTTCCTCCAAATGGTTTATCAGAAATGTCCAGAAAGTTTATACAGCACCAGAAGGATTGTGTCAACCAGGTCCTCAAAGCAGCCATGGCTATAAATGCTCAAATCATATCAGAAATGGAGATTCCTGAAGACTACATTGAATCTCTTCCTAAAGTAAGTTTTGTTAAATCACTAATCAACAGAAAAGCGAACGACGTTTAAGGGGTTTGAACACAATACCTCCTGCTCTGATAGAATCATCCTTCAATACAAAAGCGGATTTTGAAATGAATCTTCTTCACCTGGCTGAAATTAACTAGCTTTGTTTATGGTTTTACAGAATGGAAGGGCAAGCCTAGGGGATTCCATCTACAGAAGTATAACGGTTGAGTTCTTTGATCCTGATCAGTTTCTGTCCAGCATGGACCTAACATCAGAGCATAAAATCCTAGATCTGAAAGACAGAATTGAAGCTTCCATAGTGATTTGGAGGAGGAAGATGAACCAGAAAGATGGAAAATCTAGTTGGGGTTCAGCAGTGAGCTTGGAGAAGAGAGAACTTTTTGAAGAGAGAGCAGAGACCATCTTGCTAATTCTTAAGCACCGTTTCCCTGGAATCCCACAATCTTCACTAGACATCAGCAAAATTCAGTTCAATCGGGTAAAACAAATGCTCTTTCCTTAGTATTAATGAAACTTTAATCATAGTTTAAGATGAATCAGAACTTATTGTTCCCTCCATTGCAGGATATAGGGCACGCCGTTCTAGAGAGCTACTCCAGAATATTGGAAAGCTTAGCCTTCACAGTCATGTCTCGAATCGAAGACGTACTCCATGCCGATGGGTTAACCCAAAATCCTACACAGACGAGGAGGAAACCGACGAGCGAAACTCCACCGGAAAAGTCGCCGCACAAAAGAGACGAGGCTGCCCCAGAAACGCCGGTTTCAATGACATTGTTGGATTTCATGGGTTGGGGCCAGGAACAGAACGAGGCAGAGTCGAAGAAGGATTCATTCGGAAATTCAGATGATTTATATGGCGATTCAGATCTGAAACAAGCGAATAAGCTTGGGAATATAGTGACGAACAAGAAGGTTTCGTACGTGGAAAATTTGAGCGCTGTGAGAAGTCCAACGTCGCGCCATTGAAGAAGGATTGAAGAAGAAAACCCAAGAAGATGAAGATTAGATAGAGAGATAGGGAGAATTGCAAAAGAGAGAGACTGAATTTGACCTTTCTCTTTTGGCCCTTTTGGGTATTCACCATTTTTTTTCAATTACATTTGTGTTTTGTGTAAATGGGAAGAATGTGAAAAAACCTTTGATTTGGGATTTGGAATCACAACTCTTGTATTTGTGTAAATTACTCTTGTGTTTATGTAAATTCTGTATAATTTGTACAATTTGTATCATCAATG

mRNA sequence

ATGATGGTTCGTGCGATGGAGAGCGTGGAGCACGAGGGTTTCAAGTCCAGATTCTTCCATTTCAGAGGAAACCAAGAGAATGCTGGCAGACATGCCAAGAGCTTGAGCATGGAGAGCGCCAGCATGTTCGATTCTCTCCTCGAAGAAGATCTCTCGTCGAGAAGCCAAGGATCGACGCCGGTTCACAACAACTCCGCCGAGAAACAGCAATCTGGGCTGCCGCTGGCGCCATCGAATCGACCTGAAAAGGCTCTGAAGGTGAAGTTGAGCAGCAGGGACGACGACCCTGCCACTGAGAAGGAAGCCAGAGACAAGATAGCCTCAGAAATGGAACAGATCAAAGAGAGGTTTGCAAAGTTACTGTTGGGGGAAGATATGTCTGGGGGAGGGAAGGGTGTTTCCTCAGCCTTGGCATTGTCTAATGCCATCACAAACCTTGCTGCTTCTGTGTTTGGAGAACAATGGCGACTCGAGCCAATGTCAGAAGAAAGAAAAGCAAGATGGAGAAAAGAAATCGATTTGCTCTTATCTGTCACGGATTACATTGTTGAGTTTGTTCCTTCACAGCAGAAATCCAAGGATGGAACAAATATGGAGATAATGGTGACTCGGCAACGAAATGATTTGCACTTGAATATCCCAGCTTTGAGGAAGCTTGATGCCATGTTAATTGATTGTCTGGACAATTTTAAAGACCAAAATGAGTTCTACTATGTATCGAGAGACGCCGATGACTCGGAGAAAGGAAATAACAAGAGGAAAGATGATAAATGGTGGCTTCCTACAGCCAAAGTTCCTCCAAATGGTTTATCAGAAATGTCCAGAAAGTTTATACAGCACCAGAAGGATTGTGTCAACCAGGTCCTCAAAGCAGCCATGGCTATAAATGCTCAAATCATATCAGAAATGGAGATTCCTGAAGACTACATTGAATCTCTTCCTAAAAATGGAAGGGCAAGCCTAGGGGATTCCATCTACAGAAGTATAACGGTTGAGTTCTTTGATCCTGATCAGTTTCTGTCCAGCATGGACCTAACATCAGAGCATAAAATCCTAGATCTGAAAGACAGAATTGAAGCTTCCATAGTGATTTGGAGGAGGAAGATGAACCAGAAAGATGGAAAATCTAGTTGGGGTTCAGCAGTGAGCTTGGAGAAGAGAGAACTTTTTGAAGAGAGAGCAGAGACCATCTTGCTAATTCTTAAGCACCGTTTCCCTGGAATCCCACAATCTTCACTAGACATCAGCAAAATTCAGTTCAATCGGGATATAGGGCACGCCGTTCTAGAGAGCTACTCCAGAATATTGGAAAGCTTAGCCTTCACAGTCATGTCTCGAATCGAAGACGTACTCCATGCCGATGGGTTAACCCAAAATCCTACACAGACGAGGAGGAAACCGACGAGCGAAACTCCACCGGAAAAGTCGCCGCACAAAAGAGACGAGGCTGCCCCAGAAACGCCGGTTTCAATGACATTGTTGGATTTCATGGGTTGGGGCCAGGAACAGAACGAGGCAGAGTCGAAGAAGGATTCATTCGGAAATTCAGATGATTTATATGGCGATTCAGATCTGAAACAAGCGAATAAGCTTGGGAATATAGTGACGAACAAGAAGGTTTCGTACGTGGAAAATTTGAGCGCTGTGAGAAGTCCAACGTCGCGCCATTGA

Coding sequence (CDS)

ATGATGGTTCGTGCGATGGAGAGCGTGGAGCACGAGGGTTTCAAGTCCAGATTCTTCCATTTCAGAGGAAACCAAGAGAATGCTGGCAGACATGCCAAGAGCTTGAGCATGGAGAGCGCCAGCATGTTCGATTCTCTCCTCGAAGAAGATCTCTCGTCGAGAAGCCAAGGATCGACGCCGGTTCACAACAACTCCGCCGAGAAACAGCAATCTGGGCTGCCGCTGGCGCCATCGAATCGACCTGAAAAGGCTCTGAAGGTGAAGTTGAGCAGCAGGGACGACGACCCTGCCACTGAGAAGGAAGCCAGAGACAAGATAGCCTCAGAAATGGAACAGATCAAAGAGAGGTTTGCAAAGTTACTGTTGGGGGAAGATATGTCTGGGGGAGGGAAGGGTGTTTCCTCAGCCTTGGCATTGTCTAATGCCATCACAAACCTTGCTGCTTCTGTGTTTGGAGAACAATGGCGACTCGAGCCAATGTCAGAAGAAAGAAAAGCAAGATGGAGAAAAGAAATCGATTTGCTCTTATCTGTCACGGATTACATTGTTGAGTTTGTTCCTTCACAGCAGAAATCCAAGGATGGAACAAATATGGAGATAATGGTGACTCGGCAACGAAATGATTTGCACTTGAATATCCCAGCTTTGAGGAAGCTTGATGCCATGTTAATTGATTGTCTGGACAATTTTAAAGACCAAAATGAGTTCTACTATGTATCGAGAGACGCCGATGACTCGGAGAAAGGAAATAACAAGAGGAAAGATGATAAATGGTGGCTTCCTACAGCCAAAGTTCCTCCAAATGGTTTATCAGAAATGTCCAGAAAGTTTATACAGCACCAGAAGGATTGTGTCAACCAGGTCCTCAAAGCAGCCATGGCTATAAATGCTCAAATCATATCAGAAATGGAGATTCCTGAAGACTACATTGAATCTCTTCCTAAAAATGGAAGGGCAAGCCTAGGGGATTCCATCTACAGAAGTATAACGGTTGAGTTCTTTGATCCTGATCAGTTTCTGTCCAGCATGGACCTAACATCAGAGCATAAAATCCTAGATCTGAAAGACAGAATTGAAGCTTCCATAGTGATTTGGAGGAGGAAGATGAACCAGAAAGATGGAAAATCTAGTTGGGGTTCAGCAGTGAGCTTGGAGAAGAGAGAACTTTTTGAAGAGAGAGCAGAGACCATCTTGCTAATTCTTAAGCACCGTTTCCCTGGAATCCCACAATCTTCACTAGACATCAGCAAAATTCAGTTCAATCGGGATATAGGGCACGCCGTTCTAGAGAGCTACTCCAGAATATTGGAAAGCTTAGCCTTCACAGTCATGTCTCGAATCGAAGACGTACTCCATGCCGATGGGTTAACCCAAAATCCTACACAGACGAGGAGGAAACCGACGAGCGAAACTCCACCGGAAAAGTCGCCGCACAAAAGAGACGAGGCTGCCCCAGAAACGCCGGTTTCAATGACATTGTTGGATTTCATGGGTTGGGGCCAGGAACAGAACGAGGCAGAGTCGAAGAAGGATTCATTCGGAAATTCAGATGATTTATATGGCGATTCAGATCTGAAACAAGCGAATAAGCTTGGGAATATAGTGACGAACAAGAAGGTTTCGTACGTGGAAAATTTGAGCGCTGTGAGAAGTCCAACGTCGCGCCATTGA

Protein sequence

MMVRAMESVEHEGFKSRFFHFRGNQENAGRHAKSLSMESASMFDSLLEEDLSSRSQGSTPVHNNSAEKQQSGLPLAPSNRPEKALKVKLSSRDDDPATEKEARDKIASEMEQIKERFAKLLLGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSEERKARWRKEIDLLLSVTDYIVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVSRDADDSEKGNNKRKDDKWWLPTAKVPPNGLSEMSRKFIQHQKDCVNQVLKAAMAINAQIISEMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSEHKILDLKDRIEASIVIWRRKMNQKDGKSSWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFNRDIGHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNPTQTRRKPTSETPPEKSPHKRDEAAPETPVSMTLLDFMGWGQEQNEAESKKDSFGNSDDLYGDSDLKQANKLGNIVTNKKVSYVENLSAVRSPTSRH
Homology
BLAST of Spg015131 vs. NCBI nr
Match: XP_038899805.1 (rop guanine nucleotide exchange factor 12-like [Benincasa hispida])

HSP 1 Score: 954.1 bits (2465), Expect = 5.2e-274
Identity = 498/558 (89.25%), Postives = 528/558 (94.62%), Query Frame = 0

Query: 2   MVRAMESVEHEGFKSRFFHFRGNQENAGRHAKSLSMESASMFDSLLEEDLS-SRSQGSTP 61
           MVRAME  E++GF+SRFFHFRG  E+ GRHAKSLS+ESASM +S++E+DLS SRSQGSTP
Sbjct: 1   MVRAMEQ-ENDGFRSRFFHFRGTHESTGRHAKSLSIESASMLESVIEDDLSTSRSQGSTP 60

Query: 62  VHNNSAEKQQSGLPLAPSNRPEKALKVKLSSRDDDPATEKEARDKIASEMEQIKERFAKL 121
           VHNNSAEKQQ+G PLAPSNR E+A K K SS+DDDPATEKEA+DK+A EMEQ+KERFAKL
Sbjct: 61  VHNNSAEKQQAGPPLAPSNRSERAPKPKSSSKDDDPATEKEAKDKMALEMEQMKERFAKL 120

Query: 122 LLGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSEERKARWRKEIDLLLSVTD 181
           LLGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMS ERKARWRKEIDLLLSVTD
Sbjct: 121 LLGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTD 180

Query: 182 YIVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVS 241
           YIVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVS
Sbjct: 181 YIVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVS 240

Query: 242 RDADDSEKGNNKRKDDKWWLPTAKVPPNGLSEMSRKFIQHQKDCVNQVLKAAMAINAQII 301
           RDADDSEKGNNKRKDDKWWLPTAKVPPNGLS+MSRKF+Q+QKDCVNQVLKAAMAINAQII
Sbjct: 241 RDADDSEKGNNKRKDDKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQII 300

Query: 302 SEMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSEHKILDLKDRIEA 361
           SEMEIPEDYIESLPKNGRASLGDSIY+SITVEFFDPDQFLSSMDLTSEHKILDLK+RIEA
Sbjct: 301 SEMEIPEDYIESLPKNGRASLGDSIYKSITVEFFDPDQFLSSMDLTSEHKILDLKNRIEA 360

Query: 362 SIVIWRRKMNQKDGKSSWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQF 421
           SIVIWRRKMNQKDGKS+WGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQF
Sbjct: 361 SIVIWRRKMNQKDGKSTWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQF 420

Query: 422 NRDIGHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNPTQ--TRRKPTSETPPEKSPH 481
           NRD+GHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNP+Q  TRRK TSE P EKS  
Sbjct: 421 NRDVGHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNPSQIATRRKSTSEAPVEKS-E 480

Query: 482 KRDEAAPETPVSMTLLDFMGWGQEQNEAESKKDSFGNSDDLYGDSDLKQANKLGNIVTNK 541
           + +   PETP SMTLLDFMGWG +Q+E E+KKDSFGNSDDLY DSDLKQANK GNIVTNK
Sbjct: 481 EMNNNGPETPASMTLLDFMGWGADQSETETKKDSFGNSDDLYADSDLKQANKPGNIVTNK 540

Query: 542 KVSYVENLSAVRSPTSRH 557
           KVSY+ENLSAVRSPT+RH
Sbjct: 541 KVSYLENLSAVRSPTARH 556

BLAST of Spg015131 vs. NCBI nr
Match: KAA0059309.1 (rop guanine nucleotide exchange factor 12-like [Cucumis melo var. makuwa] >TYK04019.1 rop guanine nucleotide exchange factor 12-like [Cucumis melo var. makuwa])

HSP 1 Score: 944.1 bits (2439), Expect = 5.4e-271
Identity = 497/558 (89.07%), Postives = 522/558 (93.55%), Query Frame = 0

Query: 2   MVRAMESVEHEGFKSRFFHFRGNQENAGRHAKSLSMESASMFDSLLEEDLS-SRSQGSTP 61
           MVRAME  EHEGF+SRFF FRG  EN GRHAKSLS+ESA++FDS  E+DLS SRSQGSTP
Sbjct: 1   MVRAMEQ-EHEGFRSRFFLFRGTHENTGRHAKSLSVESANVFDSANEDDLSTSRSQGSTP 60

Query: 62  VHNNSAEKQQSGLPLAPSNRPEKALKVKLSSRDDDPATEKEARDKIASEMEQIKERFAKL 121
           VHNNS+EK Q+G PLAPSNR E+A K KLSS+DDD ATEKEAR+K+A EMEQ+KERFAKL
Sbjct: 61  VHNNSSEKHQAGPPLAPSNRSERAPKPKLSSKDDDLATEKEAREKMALEMEQMKERFAKL 120

Query: 122 LLGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSEERKARWRKEIDLLLSVTD 181
           LLGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMS ERKARWRKEIDLLLSVTD
Sbjct: 121 LLGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSAERKARWRKEIDLLLSVTD 180

Query: 182 YIVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVS 241
           YIVEFVPSQQKSKDG+NMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVS
Sbjct: 181 YIVEFVPSQQKSKDGSNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVS 240

Query: 242 RDADDSEKGNNKRKDDKWWLPTAKVPPNGLSEMSRKFIQHQKDCVNQVLKAAMAINAQII 301
           RDADDSEKGNNKRKDDKWWLPTAKVPPNGLS+MSRKF+Q+QKDCVNQVLKAAMAINAQII
Sbjct: 241 RDADDSEKGNNKRKDDKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQII 300

Query: 302 SEMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSEHKILDLKDRIEA 361
           SEMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSEHKILDLKDRIEA
Sbjct: 301 SEMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSEHKILDLKDRIEA 360

Query: 362 SIVIWRRKMNQKDGKSSWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQF 421
           SIVIWRRKMNQKDGKS+WGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQF
Sbjct: 361 SIVIWRRKMNQKDGKSTWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQF 420

Query: 422 NRDIGHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNPTQ--TRRKPTSETPPEKSPH 481
           NRD+GHAVLESYSRILESLAFTVMSRIEDVLHAD LTQNP+Q  TRRKPTSE P EKS  
Sbjct: 421 NRDVGHAVLESYSRILESLAFTVMSRIEDVLHADRLTQNPSQIATRRKPTSEPPMEKS-E 480

Query: 482 KRDEAAPETPVSMTLLDFMGWGQEQNEAESKKDSFGNSDDLYGDSDLKQANKLGNIVTNK 541
           + +   PETP SMTLLDFMGWGQ+QNE E KK+ FGNSDDL  DSDLKQ NK GNIVTNK
Sbjct: 481 ELNNNGPETPASMTLLDFMGWGQDQNELEMKKEWFGNSDDLNADSDLKQGNKPGNIVTNK 540

Query: 542 KVSYVENLSAVRSPTSRH 557
           KVSY+ENLSAVRSPT+RH
Sbjct: 541 KVSYLENLSAVRSPTARH 556

BLAST of Spg015131 vs. NCBI nr
Match: XP_008462168.2 (PREDICTED: LOW QUALITY PROTEIN: rop guanine nucleotide exchange factor 12-like [Cucumis melo])

HSP 1 Score: 940.6 bits (2430), Expect = 6.0e-270
Identity = 495/558 (88.71%), Postives = 521/558 (93.37%), Query Frame = 0

Query: 2   MVRAMESVEHEGFKSRFFHFRGNQENAGRHAKSLSMESASMFDSLLEEDLS-SRSQGSTP 61
           MVRAME  EHEGF+SRFF FRG  EN GRHAKSLS+ESA++FDS  E+DLS SRSQGSTP
Sbjct: 1   MVRAMEQ-EHEGFRSRFFLFRGTHENTGRHAKSLSVESANVFDSANEDDLSTSRSQGSTP 60

Query: 62  VHNNSAEKQQSGLPLAPSNRPEKALKVKLSSRDDDPATEKEARDKIASEMEQIKERFAKL 121
           VHNNS+EK Q+G PLAPSNR E+A K KLSS+DDD ATEKEAR+K+A EMEQ+KERFAKL
Sbjct: 61  VHNNSSEKHQAGPPLAPSNRSERAPKPKLSSKDDDLATEKEAREKMALEMEQMKERFAKL 120

Query: 122 LLGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSEERKARWRKEIDLLLSVTD 181
           LLGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMS ERKARWRKEIDLLLSVTD
Sbjct: 121 LLGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSAERKARWRKEIDLLLSVTD 180

Query: 182 YIVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVS 241
           YIVEFVPSQQKSKDG+NMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVS
Sbjct: 181 YIVEFVPSQQKSKDGSNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVS 240

Query: 242 RDADDSEKGNNKRKDDKWWLPTAKVPPNGLSEMSRKFIQHQKDCVNQVLKAAMAINAQII 301
           RDADDSEKGNNK KDDKWWLPTAKVPPNGLS+MSRKF+Q+QKDCVNQVLKAAMAINAQII
Sbjct: 241 RDADDSEKGNNKXKDDKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQII 300

Query: 302 SEMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSEHKILDLKDRIEA 361
           SEMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSEHKILDLKDRIEA
Sbjct: 301 SEMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSEHKILDLKDRIEA 360

Query: 362 SIVIWRRKMNQKDGKSSWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQF 421
           SIVIWRRKMNQKDGKS+WGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQF
Sbjct: 361 SIVIWRRKMNQKDGKSTWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQF 420

Query: 422 NRDIGHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNPTQ--TRRKPTSETPPEKSPH 481
           NRD+GHAVLESYSRILESLAFTVMSRIEDVLHAD LTQNP+Q  TRRKPTSE P EKS  
Sbjct: 421 NRDVGHAVLESYSRILESLAFTVMSRIEDVLHADRLTQNPSQIATRRKPTSEPPMEKS-E 480

Query: 482 KRDEAAPETPVSMTLLDFMGWGQEQNEAESKKDSFGNSDDLYGDSDLKQANKLGNIVTNK 541
           + +   PETP SMTLLDFMGWGQ+QNE E KK+ FGNSDDL  DSDLKQ NK GNIVTNK
Sbjct: 481 ELNNNGPETPASMTLLDFMGWGQDQNELEMKKEWFGNSDDLNADSDLKQGNKPGNIVTNK 540

Query: 542 KVSYVENLSAVRSPTSRH 557
           +VSY+ENLSAVRSPT+RH
Sbjct: 541 RVSYLENLSAVRSPTARH 556

BLAST of Spg015131 vs. NCBI nr
Match: XP_011659571.1 (rop guanine nucleotide exchange factor 12 [Cucumis sativus])

HSP 1 Score: 928.7 bits (2399), Expect = 2.3e-266
Identity = 492/558 (88.17%), Postives = 521/558 (93.37%), Query Frame = 0

Query: 2   MVRAMESVEHEGFKSRFFHFRGNQENAGRHAKSLSMESASMFDSLLEEDLS-SRSQGSTP 61
           MVRAME  EHEGF+SRFF FRGN EN GRHAKSLS+ESA++FDS+ E+DLS SRSQGSTP
Sbjct: 1   MVRAMEQ-EHEGFRSRFFLFRGNHENTGRHAKSLSVESANVFDSVNEDDLSTSRSQGSTP 60

Query: 62  VHNNSAEKQQSGLPLAPSNRPEKALKVKLSSRDDDPATEKEARDKIASEMEQIKERFAKL 121
           VH NS+EK Q+G PLAPSNR E+A K KLSS+D+D A EKEAR+K+A EMEQ+KERFAKL
Sbjct: 61  VH-NSSEKHQAGPPLAPSNRSERASKPKLSSKDNDLAAEKEAREKMALEMEQMKERFAKL 120

Query: 122 LLGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSEERKARWRKEIDLLLSVTD 181
           LLGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMS ERKARWRKEIDLLLSVTD
Sbjct: 121 LLGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTD 180

Query: 182 YIVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVS 241
           YIVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVS
Sbjct: 181 YIVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVS 240

Query: 242 RDADDSEKGNNKRKDDKWWLPTAKVPPNGLSEMSRKFIQHQKDCVNQVLKAAMAINAQII 301
           RDA+DSEKGNNKR +DKWWLPTAKVPPNGLS+MSRKF+Q+QKDCVNQVLKAAMAINAQII
Sbjct: 241 RDANDSEKGNNKR-NDKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQII 300

Query: 302 SEMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSEHKILDLKDRIEA 361
           SEMEIPEDYIESLPKNGRASLGDSIY+SITVEFFDPDQFLSSMDLTSEHKILDLKDRIEA
Sbjct: 301 SEMEIPEDYIESLPKNGRASLGDSIYKSITVEFFDPDQFLSSMDLTSEHKILDLKDRIEA 360

Query: 362 SIVIWRRKMNQKDGKSSWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQF 421
           SIVIWRRKMNQKDGKS+WGSAVS+EKRELFEERAETILLILKHRFPGIPQSSLDISKIQF
Sbjct: 361 SIVIWRRKMNQKDGKSTWGSAVSMEKRELFEERAETILLILKHRFPGIPQSSLDISKIQF 420

Query: 422 NRDIGHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNPTQ--TRRKPTSETPPEKSPH 481
           NRD+GHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNP+Q  TRRKPTSE P EK   
Sbjct: 421 NRDVGHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNPSQIATRRKPTSEPPMEKLEE 480

Query: 482 KRDEAAPETPVSMTLLDFMGWGQEQNEAESKKDSFGNSDDLYGDSDLKQANKLGNIVTNK 541
             +   PETP SMTLLDFMGWGQ+QNE E KK+SFGNSDDL  DSDLKQ NK GNIVTNK
Sbjct: 481 LNN--GPETPASMTLLDFMGWGQDQNETEMKKESFGNSDDLNLDSDLKQGNKAGNIVTNK 540

Query: 542 KVSYVENLSAVRSPTSRH 557
           KVSY+ENLSAVRSPT+RH
Sbjct: 541 KVSYLENLSAVRSPTARH 553

BLAST of Spg015131 vs. NCBI nr
Match: XP_023514662.1 (rop guanine nucleotide exchange factor 12-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 904.4 bits (2336), Expect = 4.7e-259
Identity = 476/557 (85.46%), Postives = 509/557 (91.38%), Query Frame = 0

Query: 2   MVRAMESVEHEGFKSRFFHFRGNQENAGRHAKSLSMESASMFDSLLEEDLSSRSQGSTPV 61
           M+ AME  EHEGFKS+  HF+G  EN+GRHAKSLS+ESASM DSL++ED +SRSQGSTPV
Sbjct: 1   MIHAMEQ-EHEGFKSKLCHFKGTHENSGRHAKSLSIESASMLDSLVDED-TSRSQGSTPV 60

Query: 62  HNNSAEKQQSGLPLAPSNRPEKALKVKLSSRDDDPATEKEARDKIASEMEQIKERFAKLL 121
           HNNSAEK     PL P+NRPEK+LK+KL ++D+D  TEKE RDK A EMEQ+KERFAKLL
Sbjct: 61  HNNSAEK-----PLTPTNRPEKSLKLKL-TKDEDAVTEKEPRDKKALEMEQMKERFAKLL 120

Query: 122 LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSEERKARWRKEIDLLLSVTDY 181
           LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMS ERK RWRKEIDLLLSVTDY
Sbjct: 121 LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSLERKTRWRKEIDLLLSVTDY 180

Query: 182 IVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVSR 241
           IVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLI CLDNFK+QNEFYYVSR
Sbjct: 181 IVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIGCLDNFKEQNEFYYVSR 240

Query: 242 DADDSEKGNNKRKDDKWWLPTAKVPPNGLSEMSRKFIQHQKDCVNQVLKAAMAINAQIIS 301
           DADDSEKGNNKRKDDKWWLPTA+VPPNGLSEM+RKF+Q+QKDCVNQVLKAAMAINAQIIS
Sbjct: 241 DADDSEKGNNKRKDDKWWLPTARVPPNGLSEMTRKFLQYQKDCVNQVLKAAMAINAQIIS 300

Query: 302 EMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSEHKILDLKDRIEAS 361
           EMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTS+HKILDLKDRIEAS
Sbjct: 301 EMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSDHKILDLKDRIEAS 360

Query: 362 IVIWRRKMNQKDGKSSWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN 421
           IVIWRRKM  KD KS+W SAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN
Sbjct: 361 IVIWRRKM--KDVKSAWASAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN 420

Query: 422 RDIGHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNPTQT--RRKPTSETPPEKSPHK 481
           RD+GHA+LESYSRILESLAFTVMSRIEDVLHAD LTQNP+QT  +RKP SETPPEK+P K
Sbjct: 421 RDVGHAILESYSRILESLAFTVMSRIEDVLHADALTQNPSQTAPKRKPASETPPEKTPRK 480

Query: 482 RDEAAPETPVSMTLLDFMGWGQEQNEAESKKDSFGNSDDLYGDSDLKQANKLGNIVTNKK 541
           R++   ETPVSMTLLDFMGWGQEQ+E E KK S G SD+L  DSD K  N  GNIVTNKK
Sbjct: 481 REDNGTETPVSMTLLDFMGWGQEQSENEPKKSSLGQSDELNADSDAKNGNTPGNIVTNKK 540

Query: 542 VSYVENLSAVRSPTSRH 557
           VSYVENLSAV+SPT+RH
Sbjct: 541 VSYVENLSAVKSPTARH 547

BLAST of Spg015131 vs. ExPASy Swiss-Prot
Match: Q9CA89 (Rop guanine nucleotide exchange factor 12 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF12 PE=1 SV=1)

HSP 1 Score: 600.1 bits (1546), Expect = 2.5e-170
Identity = 352/568 (61.97%), Postives = 417/568 (73.42%), Query Frame = 0

Query: 2   MVRAMESVEHEGFKSRFFHF--RGNQEN-AGRHAKSLSMESASMFDSLLEEDLSSRSQGS 61
           MVRA E  E E ++SR F+F  R N  N A RH KSLS+E+           L   + GS
Sbjct: 1   MVRASEQ-EQETYRSRLFNFKWRNNDNNSATRHNKSLSVETG----------LDEAATGS 60

Query: 62  TPVHNNSAEKQQSGLPLAPSNRPEKALKVKLSSRDDDPATEKEARDK-IASEMEQIKERF 121
                 +      G PL+ S   E  L    +S         +AR++ + ++MEQ+KERF
Sbjct: 61  HDAEPLTIIHPSQGPPLSRSAADEAVLAALAAS---------QARERQLLADMEQMKERF 120

Query: 122 AKLLLGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSEERKARWRKEIDLLLS 181
           +KLLLGED SGGGKGVSSALALSNAITNLAASVFGEQ RLEPM  ER+ARWRKEID LLS
Sbjct: 121 SKLLLGEDNSGGGKGVSSALALSNAITNLAASVFGEQRRLEPMPAERRARWRKEIDWLLS 180

Query: 182 VTDYIVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFY 241
           VTDY+VEF PSQQK+KDGTNMEIM TRQR DLH+NIPAL+KLDAMLIDCL+NFKDQ+EF 
Sbjct: 181 VTDYVVEFAPSQQKNKDGTNMEIMTTRQRTDLHMNIPALKKLDAMLIDCLENFKDQSEFS 240

Query: 242 YVSRDADDSEKGNNKRKDDKWWLPTAKVPPNGLSEMSRKFIQHQKDCVNQVLKAAMAINA 301
           Y+S+D+ D +    KR D+KWW+PT KVPP+GLSE SR+F+Q+QKDCVNQVLKAAMAINA
Sbjct: 241 YISKDSPDLD---GKRNDEKWWIPTVKVPPDGLSEASRRFLQYQKDCVNQVLKAAMAINA 300

Query: 302 QIISEMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSEHKILDLKDR 361
           Q++ EMEIPE YI+SLPKNGRASLGD +Y++ITV+FFDPDQFLSSMD++SEHKI+DLK+R
Sbjct: 301 QVLFEMEIPESYIDSLPKNGRASLGDQMYKNITVDFFDPDQFLSSMDMSSEHKIVDLKNR 360

Query: 362 IEASIVIWRRKMNQKDGKSS--WGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDI 421
           IEASI+IW+RKM  KD KSS  W S VSLEKRE+FEERAETILLILK R+PGI QSSLDI
Sbjct: 361 IEASIIIWKRKMVYKDNKSSAPWASGVSLEKREVFEERAETILLILKQRYPGISQSSLDI 420

Query: 422 SKIQFNRDIGHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNPTQTRRKPTSETPPEK 481
           SKIQFN D+G AVLESYSRILESLA+TV+SRI+DVL AD                    +
Sbjct: 421 SKIQFNEDVGQAVLESYSRILESLAYTVLSRIDDVLEAD--------------------R 480

Query: 482 SPHKRD---EAAPETPV-SMTLLDFMGWGQEQ---NEAESKKDSFGNSDDLYGDSDLKQA 541
           + +KR+   EA  ET V SMTL DFMGW  +Q    E ESKK       DL  D  +K+ 
Sbjct: 481 AGNKRNTPLEAEEETLVGSMTLSDFMGWDFDQAANAELESKK-------DLPDDPLIKE- 515

Query: 542 NKLGNIVTNKKVSYVENLSAVRSPTSRH 557
            KL ++VT KK SY+E L  V+SPT+RH
Sbjct: 541 -KL-SVVTTKKTSYLETLGGVKSPTARH 515

BLAST of Spg015131 vs. ExPASy Swiss-Prot
Match: Q9M811 (Rop guanine nucleotide exchange factor 11 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF11 PE=1 SV=2)

HSP 1 Score: 594.7 bits (1532), Expect = 1.1e-168
Identity = 343/561 (61.14%), Postives = 408/561 (72.73%), Query Frame = 0

Query: 2   MVRAMESVEHEGFKSRFFHFRG-NQENAGRHAKSLSMESASMF---DSLLEEDLSSRSQG 61
           MV+AME  E E +KSR FHF+  N+ +A RH KS S + A      D+L ++D       
Sbjct: 1   MVKAMEQ-EQETYKSRLFHFKNMNENSASRHVKSWSSDCAMRMDGSDNLDDDDNDMMMFR 60

Query: 62  STPVHNNSAEKQQSGLPLAPSNRPEKALKVKLSSRDDDPATEKEARDKIASEMEQIKERF 121
           S P    S ++    +     NR +K  +V  SS D       EA   + + MEQ+KE+F
Sbjct: 61  SQPGKCGSVDRPSLPIGGVTPNRNDKLPRV--SSSD-----SMEALIILQAAMEQMKEKF 120

Query: 122 AKLLLGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSEERKARWRKEIDLLLS 181
           +KLLLGEDMSGGGKGVSSALALSNAITNLAAS FGEQ RLEPM+ +RK RWR+EI  L+S
Sbjct: 121 SKLLLGEDMSGGGKGVSSALALSNAITNLAASAFGEQRRLEPMAVDRKTRWRREIGWLIS 180

Query: 182 VTDYIVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFY 241
           V DYIVEF P+QQ +KDGT+ME+M TRQR DL  NIPAL+KLDAML+DCLD FKDQ+EFY
Sbjct: 181 VADYIVEFAPTQQTNKDGTSMEVMSTRQRTDLLCNIPALKKLDAMLLDCLDKFKDQDEFY 240

Query: 242 YVSRDADDSEKGNNKRKDDKWWLPTAKVPPNGLSEMSRKFIQHQKDCVNQVLKAAMAINA 301
           YV +D+ DS      R D+KWWLP  KVPPNGLSE+SR+F+Q QK+CVNQVLKAAMAINA
Sbjct: 241 YVKKDSPDS---CETRNDEKWWLPAVKVPPNGLSEISRRFLQSQKECVNQVLKAAMAINA 300

Query: 302 QIISEMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSEHKILDLKDR 361
           Q++SEMEIPE Y+ESLPKNGRASLGD IYR ITVE FD DQFL  MDL+SEHKILDLK+R
Sbjct: 301 QVLSEMEIPESYLESLPKNGRASLGDVIYRMITVEMFDADQFLIEMDLSSEHKILDLKNR 360

Query: 362 IEASIVIWRRKMNQKDGKSSWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISK 421
           IEASIVIW+RKM QKD KS WGS VS+EKRE FEERAETILL+LK  FPGI QSSLDISK
Sbjct: 361 IEASIVIWKRKMVQKDTKSPWGSTVSIEKREQFEERAETILLLLKQGFPGISQSSLDISK 420

Query: 422 IQFNRDIGHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNPTQTRRKPTSETPPEKSP 481
           IQFNRD+G A+LESYSR+LESLA TVMSRIEDVL+AD LTQ PT         +  E   
Sbjct: 421 IQFNRDVGLAILESYSRVLESLAHTVMSRIEDVLYADQLTQEPTNNAPSKNRYSLKENEK 480

Query: 482 HKRDEAA-PETPVSMTLLDFMGWGQEQNEAESKKDSFGNSDDLYGDSDLKQANKLGNIVT 541
            + +  +  E   S TL D M WG + N  E KK+SF      +GD +    +K+  I+T
Sbjct: 481 LREERLSFTEDMASGTLSDVMQWGNKNN--EMKKESF------FGDREKPLLSKVTGIMT 540

Query: 542 -NKKVSYVENLSAVRSPTSRH 557
            NKK SY++NL A+RSPT+R+
Sbjct: 541 NNKKSSYLDNLGAMRSPTARY 542

BLAST of Spg015131 vs. ExPASy Swiss-Prot
Match: Q9LV40 (Rho guanine nucleotide exchange factor 8 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF8 PE=1 SV=1)

HSP 1 Score: 557.4 bits (1435), Expect = 1.9e-157
Identity = 316/536 (58.96%), Postives = 387/536 (72.20%), Query Frame = 0

Query: 33  KSLSMESASMFDSLLEEDLSSRSQGST-PVHNNSAEKQQSGLPLAPSNRPEKALKVKLSS 92
           + LS   +  F  + +   + + Q  T  V N  +   +S  P + ++       V+ S 
Sbjct: 7   RGLSASKSFNFKRMFDSSSTKQQQSQTIVVENGDSHIVESNTPESQNSDSFVESPVESSL 66

Query: 93  RDDDPATEKEAR-DKIASEMEQIKERFAKLLLGEDMSGGGKGVSSALALSNAITNLAASV 152
               P T    R ++  ++ME +K+RFAKLLLGEDMSGGGKGVSSALALSNAITNLAAS+
Sbjct: 67  PMISPLTRPGKRSERQQADMEMMKDRFAKLLLGEDMSGGGKGVSSALALSNAITNLAASI 126

Query: 153 FGEQWRLEPMSEERKARWRKEIDLLLSVTDYIVEFVPSQQKSKDGTNMEIMVTRQRNDLH 212
           FGEQ +L+PM ++R+ARW+KEID LLSVTD+IVEFVPSQQ SKDG   EIMVTRQR DL 
Sbjct: 127 FGEQTKLQPMPQDRQARWKKEIDWLLSVTDHIVEFVPSQQTSKDGVCTEIMVTRQRGDLL 186

Query: 213 LNIPALRKLDAMLIDCLDNFKDQNEFYYVSRDADDSEKGNNKRKDDKWWLPTAKVPPNGL 272
           +NIPALRKLDAMLID LDNF+  NEF+YVSRD+++ ++  N R +DKWWLP  KVPP GL
Sbjct: 187 MNIPALRKLDAMLIDTLDNFRGHNEFWYVSRDSEEGQQARNDRTNDKWWLPPVKVPPGGL 246

Query: 273 SEMSRKFIQHQKDCVNQVLKAAMAINAQIISEMEIPEDYIESLPKNGRASLGDSIYRSIT 332
           SE SR+ +  QKD V QV KAAMAINAQ++SEMEIPE YI+SLPKNGRASLGDSIY+SIT
Sbjct: 247 SEPSRRMLYFQKDSVTQVQKAAMAINAQVLSEMEIPESYIDSLPKNGRASLGDSIYKSIT 306

Query: 333 VEFFDPDQFLSSMDLTSEHKILDLKDRIEASIVIWRRKMNQKDGKSSWGSAVSLEKRELF 392
            E+FDP+QFL+ +D+++EHK+LDLK+RIEAS+VIW+RK++ KD KSSWGSAVSLEKRELF
Sbjct: 307 EEWFDPEQFLAMLDMSTEHKVLDLKNRIEASVVIWKRKLHTKDTKSSWGSAVSLEKRELF 366

Query: 393 EERAETILLILKHRFPGIPQSSLDISKIQFNRDIGHAVLESYSRILESLAFTVMSRIEDV 452
           EERAETIL++LK +FPG+PQSSLDISKIQFN+D+G AVLESYSRILESLA+TVMSRIEDV
Sbjct: 367 EERAETILVLLKQKFPGLPQSSLDISKIQFNKDVGQAVLESYSRILESLAYTVMSRIEDV 426

Query: 453 LHADGLTQNPTQTRRKP-----TSETPPEK--SPHKRDEAAPETPVSMTLLDFMGWGQEQ 512
           L+ D L    T    +      T+ET  E   S +  +EA    P S TLLDFMGW    
Sbjct: 427 LYTDTLALKQTLLAEETSDGGRTTETDSESAGSSNSGEEAEKHDPHSKTLLDFMGW---- 486

Query: 513 NEAESKKDSFGNSDDLYGDSDLKQANKLGNIVTNKKVSY---VENLSAVRSPTSRH 557
           N+  SK           GD   K  N     +T KK+SY   +ENL+  RSP  RH
Sbjct: 487 NDNSSKG----------GDKPTKSPN-----LTPKKLSYLEKLENLNGFRSPKDRH 523

BLAST of Spg015131 vs. ExPASy Swiss-Prot
Match: Q9SVQ3 (Rop guanine nucleotide exchange factor 9 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF9 PE=1 SV=1)

HSP 1 Score: 542.3 bits (1396), Expect = 6.2e-153
Identity = 300/514 (58.37%), Postives = 372/514 (72.37%), Query Frame = 0

Query: 63  NNSAEKQQSGLPLAPSNRPEKALKVKL------SSRDDDPATEKEARDKIASEMEQIKER 122
           ++S  K+Q    LA +  PE   +  L      +SR          +DK  SE E +K+R
Sbjct: 22  DSSPGKEQQQPHLAETTMPESQTQDSLGGSPVETSRPMTSRLISRRQDKQQSETEMMKDR 81

Query: 123 FAKLLLGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSEERKARWRKEIDLLL 182
           F KLLLGEDMSGGGKGVSSALALSNAITNLAAS+FGEQ +L+PM+ +R+ARW+KEID LL
Sbjct: 82  FTKLLLGEDMSGGGKGVSSALALSNAITNLAASIFGEQTKLQPMAPDRRARWKKEIDWLL 141

Query: 183 SVTDYIVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEF 242
           SVTD+IVEFVPSQQ SK+G   EIMVTRQR DL +NIPALRKLDAMLID LDNF+  NEF
Sbjct: 142 SVTDHIVEFVPSQQISKEGVCTEIMVTRQRGDLLMNIPALRKLDAMLIDTLDNFRGHNEF 201

Query: 243 YYVSRDADDSEKGNNKRKDDKWWLPTAKVPPNGLSEMSRKFIQHQKDCVNQVLKAAMAIN 302
           +YVSRD+++ ++  N+R  DKWWLP  KVPPNGLSE +R+ +  QKD V+QV KAAMAIN
Sbjct: 202 WYVSRDSEEGKQARNERTKDKWWLPPVKVPPNGLSESARRMLHFQKDSVSQVQKAAMAIN 261

Query: 303 AQIISEMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSEHKILDLKD 362
           AQ++SEM IP+ YIESLPKNGR SLGDS+Y+SIT E+FDP+QFLS++DL++EHK+LD+K+
Sbjct: 262 AQVLSEMAIPDSYIESLPKNGRVSLGDSLYKSITEEWFDPEQFLSTLDLSTEHKVLDVKN 321

Query: 363 RIEASIVIWRRKMNQKDGKSSWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDIS 422
           RIEASIVIW+RK++ KD KSSWGSAVSLEKRELFEERAETIL++LK +FPG+PQSSLDIS
Sbjct: 322 RIEASIVIWKRKLHLKDNKSSWGSAVSLEKRELFEERAETILVLLKQKFPGLPQSSLDIS 381

Query: 423 KIQFNRDIGHAVLESYSRILESLAFTVMSRIEDVLHADGL-----TQNPTQTRRKPTSET 482
           KIQ+N+D+GHAVLESYSRILESL +T MSRI+DVL+AD L     T   T    K  +ET
Sbjct: 382 KIQYNKDVGHAVLESYSRILESLGYTEMSRIDDVLYADSLARKQCTGEETSDGGKIATET 441

Query: 483 PPEK------SPHKRDEAAPETPVSMTLLDFMGWGQEQNEAESKKDSFGNSDDLYGDSDL 542
                     S  + ++   +     TLLDF+GW    ++ +S+K               
Sbjct: 442 DSASAGSSNYSGEEIEKLESQNSSKTTLLDFIGWSDNSSKGQSEKPPKSPR--------- 501

Query: 543 KQANKLGNIVTNKKVSY---VENLSAVRSPTSRH 557
                    +T KK+SY   +ENL+  RSP  RH
Sbjct: 502 ---------MTPKKLSYLEKLENLNGFRSPKDRH 517

BLAST of Spg015131 vs. ExPASy Swiss-Prot
Match: Q1KS66 (Rop guanine nucleotide exchange factor 10 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF10 PE=2 SV=1)

HSP 1 Score: 491.5 bits (1264), Expect = 1.3e-137
Identity = 264/466 (56.65%), Postives = 347/466 (74.46%), Query Frame = 0

Query: 96  PATEKEARDKIASEMEQIKERFAKLLLGEDMSGGGKGVSSALALSNAITNLAASVFGEQW 155
           P+T +  +++  S+ME +KERFAKLLLGEDMSGGG G +SALALSNAIT LA S+FGEQ 
Sbjct: 32  PSTRRGKQNR-RSDMEVMKERFAKLLLGEDMSGGGTGETSALALSNAITKLADSMFGEQM 91

Query: 156 RLEPMSEERKARWRKEIDLLLSVTDYIVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPA 215
           +L+PM  E K  WRKE+  LLSV D+IV+FVPS+Q  K+G   EIMVT+QR+DL  NIPA
Sbjct: 92  KLQPMYPETKENWRKEMGWLLSVIDHIVQFVPSRQMGKNGQFTEIMVTKQRDDLLTNIPA 151

Query: 216 LRKLDAMLIDCLDNFKDQNEFYYVSRDADDSEKGNNKRKDDKWWLPTAKVPPNGLSEMSR 275
           LRKLD++L++ LDNFKDQ +F+YV RD +D++   + R+D+ WWLP  KVP +GLSE SR
Sbjct: 152 LRKLDSVLLETLDNFKDQKDFWYVPRDMEDADHNGDWRRDENWWLPVVKVPTDGLSEESR 211

Query: 276 KFIQHQKDCVNQVLKAAMAINAQIISEMEIPEDYIESLPKNGRASLGDSIYRSITVEFFD 335
           +++Q+QKD V QVLKAA AINA ++SEM +PE+YI+SLPKNG+ SLGD +Y+SIT E FD
Sbjct: 212 RWLQNQKDSVAQVLKAATAINAHVLSEMHVPENYIDSLPKNGKTSLGDFLYKSITEESFD 271

Query: 336 PDQFLSSMDLTSEHKILDLKDRIEASIVIWRRKM--NQKDGKSSWGSAVSLEKRELFEER 395
           PD F+S +DL++EHK+LDLK+RIEAS+VIW+RKM   +KDGKS WGS VSLEKRELFE R
Sbjct: 272 PDYFVSFLDLSTEHKVLDLKNRIEASMVIWKRKMCQKEKDGKSQWGSTVSLEKRELFEVR 331

Query: 396 AETILLILKHRFPGIPQSSLDISKIQFNRDIGHAVLESYSRILESLAFTVMSRIEDVLHA 455
           AETIL++LK +FPGIPQSSL++SKI+ N+D+G A+LESYSR+LESLA  +MSRIEDVL A
Sbjct: 332 AETILVMLKQQFPGIPQSSLEVSKIKNNKDVGQAILESYSRVLESLASKIMSRIEDVLEA 391

Query: 456 DGLTQNPTQTRRKPTSETPPEKSPHKRDE-AAPETPVSMTLLDFMGWGQEQNEAESKKDS 515
           D L Q       +  SE+  E    + ++  A ETP S  L DF+GW   +  +++KK S
Sbjct: 392 DRLVQRQLMGEAETRSESEAESEYEETEKVVAAETPNSRKLSDFIGW---RLSSDTKKHS 451

Query: 516 FGNSDDLYGDSDLKQANKL--GNIVTNKKVSYVENLSAVRSPTSRH 557
             +  + +   + ++   +        KK SY+  L  +RSP+ RH
Sbjct: 452 SMSDIEFFHKVEQEKEKPMMKSPRALPKKFSYLAKLENMRSPSDRH 493

BLAST of Spg015131 vs. ExPASy TrEMBL
Match: A0A5D3C0X6 (Rop guanine nucleotide exchange factor 12-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G002180 PE=4 SV=1)

HSP 1 Score: 944.1 bits (2439), Expect = 2.6e-271
Identity = 497/558 (89.07%), Postives = 522/558 (93.55%), Query Frame = 0

Query: 2   MVRAMESVEHEGFKSRFFHFRGNQENAGRHAKSLSMESASMFDSLLEEDLS-SRSQGSTP 61
           MVRAME  EHEGF+SRFF FRG  EN GRHAKSLS+ESA++FDS  E+DLS SRSQGSTP
Sbjct: 1   MVRAMEQ-EHEGFRSRFFLFRGTHENTGRHAKSLSVESANVFDSANEDDLSTSRSQGSTP 60

Query: 62  VHNNSAEKQQSGLPLAPSNRPEKALKVKLSSRDDDPATEKEARDKIASEMEQIKERFAKL 121
           VHNNS+EK Q+G PLAPSNR E+A K KLSS+DDD ATEKEAR+K+A EMEQ+KERFAKL
Sbjct: 61  VHNNSSEKHQAGPPLAPSNRSERAPKPKLSSKDDDLATEKEAREKMALEMEQMKERFAKL 120

Query: 122 LLGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSEERKARWRKEIDLLLSVTD 181
           LLGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMS ERKARWRKEIDLLLSVTD
Sbjct: 121 LLGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSAERKARWRKEIDLLLSVTD 180

Query: 182 YIVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVS 241
           YIVEFVPSQQKSKDG+NMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVS
Sbjct: 181 YIVEFVPSQQKSKDGSNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVS 240

Query: 242 RDADDSEKGNNKRKDDKWWLPTAKVPPNGLSEMSRKFIQHQKDCVNQVLKAAMAINAQII 301
           RDADDSEKGNNKRKDDKWWLPTAKVPPNGLS+MSRKF+Q+QKDCVNQVLKAAMAINAQII
Sbjct: 241 RDADDSEKGNNKRKDDKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQII 300

Query: 302 SEMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSEHKILDLKDRIEA 361
           SEMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSEHKILDLKDRIEA
Sbjct: 301 SEMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSEHKILDLKDRIEA 360

Query: 362 SIVIWRRKMNQKDGKSSWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQF 421
           SIVIWRRKMNQKDGKS+WGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQF
Sbjct: 361 SIVIWRRKMNQKDGKSTWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQF 420

Query: 422 NRDIGHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNPTQ--TRRKPTSETPPEKSPH 481
           NRD+GHAVLESYSRILESLAFTVMSRIEDVLHAD LTQNP+Q  TRRKPTSE P EKS  
Sbjct: 421 NRDVGHAVLESYSRILESLAFTVMSRIEDVLHADRLTQNPSQIATRRKPTSEPPMEKS-E 480

Query: 482 KRDEAAPETPVSMTLLDFMGWGQEQNEAESKKDSFGNSDDLYGDSDLKQANKLGNIVTNK 541
           + +   PETP SMTLLDFMGWGQ+QNE E KK+ FGNSDDL  DSDLKQ NK GNIVTNK
Sbjct: 481 ELNNNGPETPASMTLLDFMGWGQDQNELEMKKEWFGNSDDLNADSDLKQGNKPGNIVTNK 540

Query: 542 KVSYVENLSAVRSPTSRH 557
           KVSY+ENLSAVRSPT+RH
Sbjct: 541 KVSYLENLSAVRSPTARH 556

BLAST of Spg015131 vs. ExPASy TrEMBL
Match: A0A1S3CGA0 (LOW QUALITY PROTEIN: rop guanine nucleotide exchange factor 12-like OS=Cucumis melo OX=3656 GN=LOC103500592 PE=4 SV=1)

HSP 1 Score: 940.6 bits (2430), Expect = 2.9e-270
Identity = 495/558 (88.71%), Postives = 521/558 (93.37%), Query Frame = 0

Query: 2   MVRAMESVEHEGFKSRFFHFRGNQENAGRHAKSLSMESASMFDSLLEEDLS-SRSQGSTP 61
           MVRAME  EHEGF+SRFF FRG  EN GRHAKSLS+ESA++FDS  E+DLS SRSQGSTP
Sbjct: 1   MVRAMEQ-EHEGFRSRFFLFRGTHENTGRHAKSLSVESANVFDSANEDDLSTSRSQGSTP 60

Query: 62  VHNNSAEKQQSGLPLAPSNRPEKALKVKLSSRDDDPATEKEARDKIASEMEQIKERFAKL 121
           VHNNS+EK Q+G PLAPSNR E+A K KLSS+DDD ATEKEAR+K+A EMEQ+KERFAKL
Sbjct: 61  VHNNSSEKHQAGPPLAPSNRSERAPKPKLSSKDDDLATEKEAREKMALEMEQMKERFAKL 120

Query: 122 LLGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSEERKARWRKEIDLLLSVTD 181
           LLGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMS ERKARWRKEIDLLLSVTD
Sbjct: 121 LLGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSAERKARWRKEIDLLLSVTD 180

Query: 182 YIVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVS 241
           YIVEFVPSQQKSKDG+NMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVS
Sbjct: 181 YIVEFVPSQQKSKDGSNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVS 240

Query: 242 RDADDSEKGNNKRKDDKWWLPTAKVPPNGLSEMSRKFIQHQKDCVNQVLKAAMAINAQII 301
           RDADDSEKGNNK KDDKWWLPTAKVPPNGLS+MSRKF+Q+QKDCVNQVLKAAMAINAQII
Sbjct: 241 RDADDSEKGNNKXKDDKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQII 300

Query: 302 SEMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSEHKILDLKDRIEA 361
           SEMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSEHKILDLKDRIEA
Sbjct: 301 SEMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSEHKILDLKDRIEA 360

Query: 362 SIVIWRRKMNQKDGKSSWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQF 421
           SIVIWRRKMNQKDGKS+WGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQF
Sbjct: 361 SIVIWRRKMNQKDGKSTWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQF 420

Query: 422 NRDIGHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNPTQ--TRRKPTSETPPEKSPH 481
           NRD+GHAVLESYSRILESLAFTVMSRIEDVLHAD LTQNP+Q  TRRKPTSE P EKS  
Sbjct: 421 NRDVGHAVLESYSRILESLAFTVMSRIEDVLHADRLTQNPSQIATRRKPTSEPPMEKS-E 480

Query: 482 KRDEAAPETPVSMTLLDFMGWGQEQNEAESKKDSFGNSDDLYGDSDLKQANKLGNIVTNK 541
           + +   PETP SMTLLDFMGWGQ+QNE E KK+ FGNSDDL  DSDLKQ NK GNIVTNK
Sbjct: 481 ELNNNGPETPASMTLLDFMGWGQDQNELEMKKEWFGNSDDLNADSDLKQGNKPGNIVTNK 540

Query: 542 KVSYVENLSAVRSPTSRH 557
           +VSY+ENLSAVRSPT+RH
Sbjct: 541 RVSYLENLSAVRSPTARH 556

BLAST of Spg015131 vs. ExPASy TrEMBL
Match: A0A0A0K7D8 (PRONE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G446980 PE=4 SV=1)

HSP 1 Score: 928.7 bits (2399), Expect = 1.1e-266
Identity = 492/558 (88.17%), Postives = 521/558 (93.37%), Query Frame = 0

Query: 2   MVRAMESVEHEGFKSRFFHFRGNQENAGRHAKSLSMESASMFDSLLEEDLS-SRSQGSTP 61
           MVRAME  EHEGF+SRFF FRGN EN GRHAKSLS+ESA++FDS+ E+DLS SRSQGSTP
Sbjct: 1   MVRAMEQ-EHEGFRSRFFLFRGNHENTGRHAKSLSVESANVFDSVNEDDLSTSRSQGSTP 60

Query: 62  VHNNSAEKQQSGLPLAPSNRPEKALKVKLSSRDDDPATEKEARDKIASEMEQIKERFAKL 121
           VH NS+EK Q+G PLAPSNR E+A K KLSS+D+D A EKEAR+K+A EMEQ+KERFAKL
Sbjct: 61  VH-NSSEKHQAGPPLAPSNRSERASKPKLSSKDNDLAAEKEAREKMALEMEQMKERFAKL 120

Query: 122 LLGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSEERKARWRKEIDLLLSVTD 181
           LLGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMS ERKARWRKEIDLLLSVTD
Sbjct: 121 LLGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSVERKARWRKEIDLLLSVTD 180

Query: 182 YIVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVS 241
           YIVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVS
Sbjct: 181 YIVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVS 240

Query: 242 RDADDSEKGNNKRKDDKWWLPTAKVPPNGLSEMSRKFIQHQKDCVNQVLKAAMAINAQII 301
           RDA+DSEKGNNKR +DKWWLPTAKVPPNGLS+MSRKF+Q+QKDCVNQVLKAAMAINAQII
Sbjct: 241 RDANDSEKGNNKR-NDKWWLPTAKVPPNGLSDMSRKFLQYQKDCVNQVLKAAMAINAQII 300

Query: 302 SEMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSEHKILDLKDRIEA 361
           SEMEIPEDYIESLPKNGRASLGDSIY+SITVEFFDPDQFLSSMDLTSEHKILDLKDRIEA
Sbjct: 301 SEMEIPEDYIESLPKNGRASLGDSIYKSITVEFFDPDQFLSSMDLTSEHKILDLKDRIEA 360

Query: 362 SIVIWRRKMNQKDGKSSWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQF 421
           SIVIWRRKMNQKDGKS+WGSAVS+EKRELFEERAETILLILKHRFPGIPQSSLDISKIQF
Sbjct: 361 SIVIWRRKMNQKDGKSTWGSAVSMEKRELFEERAETILLILKHRFPGIPQSSLDISKIQF 420

Query: 422 NRDIGHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNPTQ--TRRKPTSETPPEKSPH 481
           NRD+GHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNP+Q  TRRKPTSE P EK   
Sbjct: 421 NRDVGHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNPSQIATRRKPTSEPPMEKLEE 480

Query: 482 KRDEAAPETPVSMTLLDFMGWGQEQNEAESKKDSFGNSDDLYGDSDLKQANKLGNIVTNK 541
             +   PETP SMTLLDFMGWGQ+QNE E KK+SFGNSDDL  DSDLKQ NK GNIVTNK
Sbjct: 481 LNN--GPETPASMTLLDFMGWGQDQNETEMKKESFGNSDDLNLDSDLKQGNKAGNIVTNK 540

Query: 542 KVSYVENLSAVRSPTSRH 557
           KVSY+ENLSAVRSPT+RH
Sbjct: 541 KVSYLENLSAVRSPTARH 553

BLAST of Spg015131 vs. ExPASy TrEMBL
Match: A0A6J1HL16 (rop guanine nucleotide exchange factor 12-like OS=Cucurbita moschata OX=3662 GN=LOC111464520 PE=4 SV=1)

HSP 1 Score: 904.0 bits (2335), Expect = 3.0e-259
Identity = 475/557 (85.28%), Postives = 509/557 (91.38%), Query Frame = 0

Query: 2   MVRAMESVEHEGFKSRFFHFRGNQENAGRHAKSLSMESASMFDSLLEEDLSSRSQGSTPV 61
           M+ AME  EHEGFKS+  HF+G  EN+GRHAKSLS+ESASM DSL++ED +SRSQGSTPV
Sbjct: 1   MIHAMEQ-EHEGFKSKLCHFKGTHENSGRHAKSLSIESASMLDSLVDED-TSRSQGSTPV 60

Query: 62  HNNSAEKQQSGLPLAPSNRPEKALKVKLSSRDDDPATEKEARDKIASEMEQIKERFAKLL 121
           HNNSAEK     PL P+NRPEK+LK+KL ++D+D  TEKE RDK A EMEQ+KERFAKLL
Sbjct: 61  HNNSAEK-----PLTPTNRPEKSLKLKL-TKDEDAVTEKEPRDKKALEMEQMKERFAKLL 120

Query: 122 LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSEERKARWRKEIDLLLSVTDY 181
           LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMS ERK RWRKEIDLLLSVTDY
Sbjct: 121 LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSLERKTRWRKEIDLLLSVTDY 180

Query: 182 IVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVSR 241
           IVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLI CLDNFK+QNEFYYVSR
Sbjct: 181 IVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIGCLDNFKEQNEFYYVSR 240

Query: 242 DADDSEKGNNKRKDDKWWLPTAKVPPNGLSEMSRKFIQHQKDCVNQVLKAAMAINAQIIS 301
           DADDSEKGNNKRKDDKWWLPTA+VPPNGLSEM+RKF+Q+QKDCVNQVLKAAMAINAQIIS
Sbjct: 241 DADDSEKGNNKRKDDKWWLPTARVPPNGLSEMTRKFLQYQKDCVNQVLKAAMAINAQIIS 300

Query: 302 EMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSEHKILDLKDRIEAS 361
           EMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTS+HKILDLKDRIEAS
Sbjct: 301 EMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSDHKILDLKDRIEAS 360

Query: 362 IVIWRRKMNQKDGKSSWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN 421
           IVIWRRKM  KD KS+W SAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN
Sbjct: 361 IVIWRRKM--KDVKSAWASAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN 420

Query: 422 RDIGHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNPTQT--RRKPTSETPPEKSPHK 481
           RD+GHA+LESYSRILESLAFTVMSRIEDVLHAD LTQNP+QT  +RKP SETPPEK+P K
Sbjct: 421 RDVGHAILESYSRILESLAFTVMSRIEDVLHADALTQNPSQTASKRKPASETPPEKTPRK 480

Query: 482 RDEAAPETPVSMTLLDFMGWGQEQNEAESKKDSFGNSDDLYGDSDLKQANKLGNIVTNKK 541
           R++   ETPVSMTLLDFMGWGQEQ+E E KK S G SD+L  DSD K  N  GN+VTNKK
Sbjct: 481 REDNGTETPVSMTLLDFMGWGQEQSENEPKKSSLGQSDELNADSDAKNGNTPGNVVTNKK 540

Query: 542 VSYVENLSAVRSPTSRH 557
           VSYVENLSAV+SPT+RH
Sbjct: 541 VSYVENLSAVKSPTARH 547

BLAST of Spg015131 vs. ExPASy TrEMBL
Match: A0A6J1KI31 (rop guanine nucleotide exchange factor 12-like OS=Cucurbita maxima OX=3661 GN=LOC111494607 PE=4 SV=1)

HSP 1 Score: 899.4 bits (2323), Expect = 7.4e-258
Identity = 474/557 (85.10%), Postives = 507/557 (91.02%), Query Frame = 0

Query: 2   MVRAMESVEHEGFKSRFFHFRGNQENAGRHAKSLSMESASMFDSLLEEDLSSRSQGSTPV 61
           M+ AME  EHEGFKS+  HF+G  EN+GRHAKSLS+E A M DSL++ED +SRSQGSTPV
Sbjct: 1   MIHAMEQ-EHEGFKSKLCHFKGTHENSGRHAKSLSIEGAGMLDSLVDED-TSRSQGSTPV 60

Query: 62  HNNSAEKQQSGLPLAPSNRPEKALKVKLSSRDDDPATEKEARDKIASEMEQIKERFAKLL 121
           HNNSAEK     PL P+NRPEK+LK+KL ++D+D  TEKE RDK A EMEQ+KERFAKLL
Sbjct: 61  HNNSAEK-----PLTPTNRPEKSLKLKL-TKDEDAVTEKEPRDKKALEMEQMKERFAKLL 120

Query: 122 LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSEERKARWRKEIDLLLSVTDY 181
           LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMS ERK RWRKEIDLLLSVTDY
Sbjct: 121 LGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSLERKTRWRKEIDLLLSVTDY 180

Query: 182 IVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVSR 241
           IVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLI CLDNFK+QNEFYYVSR
Sbjct: 181 IVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIGCLDNFKEQNEFYYVSR 240

Query: 242 DADDSEKGNNKRKDDKWWLPTAKVPPNGLSEMSRKFIQHQKDCVNQVLKAAMAINAQIIS 301
           DADDSEKGNNKRKDDKWWLPTA+VPPNGLSEMSRKF+Q+QKDCVNQVLKAAMAINAQIIS
Sbjct: 241 DADDSEKGNNKRKDDKWWLPTARVPPNGLSEMSRKFLQYQKDCVNQVLKAAMAINAQIIS 300

Query: 302 EMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSEHKILDLKDRIEAS 361
           EMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTS+HKILDLKDRIEAS
Sbjct: 301 EMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSDHKILDLKDRIEAS 360

Query: 362 IVIWRRKMNQKDGKSSWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN 421
           IVIWRRKM  KD KS+W SAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN
Sbjct: 361 IVIWRRKM--KDVKSAWASAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFN 420

Query: 422 RDIGHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNPTQT--RRKPTSETPPEKSPHK 481
           RD+GHA+LESYSRILESLAFTVMSRIEDVLHAD +TQNP+QT  +RKP SETPPEK+P K
Sbjct: 421 RDVGHAILESYSRILESLAFTVMSRIEDVLHADAVTQNPSQTAPKRKPASETPPEKTPCK 480

Query: 482 RDEAAPETPVSMTLLDFMGWGQEQNEAESKKDSFGNSDDLYGDSDLKQANKLGNIVTNKK 541
           R++   ETPVSMTLLDFMGWGQEQ+E E KK S G SD+L  DSD K  N  GNIVTNKK
Sbjct: 481 REDNGTETPVSMTLLDFMGWGQEQSENEPKKASLGQSDELNADSDAKNGNTPGNIVTNKK 540

Query: 542 VSYVENLSAVRSPTSRH 557
           VSYVENLSAV+SPT+RH
Sbjct: 541 VSYVENLSAVKSPTARH 547

BLAST of Spg015131 vs. TAIR 10
Match: AT1G79860.1 (RHO guanyl-nucleotide exchange factor 12 )

HSP 1 Score: 600.1 bits (1546), Expect = 1.8e-171
Identity = 352/568 (61.97%), Postives = 417/568 (73.42%), Query Frame = 0

Query: 2   MVRAMESVEHEGFKSRFFHF--RGNQEN-AGRHAKSLSMESASMFDSLLEEDLSSRSQGS 61
           MVRA E  E E ++SR F+F  R N  N A RH KSLS+E+           L   + GS
Sbjct: 1   MVRASEQ-EQETYRSRLFNFKWRNNDNNSATRHNKSLSVETG----------LDEAATGS 60

Query: 62  TPVHNNSAEKQQSGLPLAPSNRPEKALKVKLSSRDDDPATEKEARDK-IASEMEQIKERF 121
                 +      G PL+ S   E  L    +S         +AR++ + ++MEQ+KERF
Sbjct: 61  HDAEPLTIIHPSQGPPLSRSAADEAVLAALAAS---------QARERQLLADMEQMKERF 120

Query: 122 AKLLLGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSEERKARWRKEIDLLLS 181
           +KLLLGED SGGGKGVSSALALSNAITNLAASVFGEQ RLEPM  ER+ARWRKEID LLS
Sbjct: 121 SKLLLGEDNSGGGKGVSSALALSNAITNLAASVFGEQRRLEPMPAERRARWRKEIDWLLS 180

Query: 182 VTDYIVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFY 241
           VTDY+VEF PSQQK+KDGTNMEIM TRQR DLH+NIPAL+KLDAMLIDCL+NFKDQ+EF 
Sbjct: 181 VTDYVVEFAPSQQKNKDGTNMEIMTTRQRTDLHMNIPALKKLDAMLIDCLENFKDQSEFS 240

Query: 242 YVSRDADDSEKGNNKRKDDKWWLPTAKVPPNGLSEMSRKFIQHQKDCVNQVLKAAMAINA 301
           Y+S+D+ D +    KR D+KWW+PT KVPP+GLSE SR+F+Q+QKDCVNQVLKAAMAINA
Sbjct: 241 YISKDSPDLD---GKRNDEKWWIPTVKVPPDGLSEASRRFLQYQKDCVNQVLKAAMAINA 300

Query: 302 QIISEMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSEHKILDLKDR 361
           Q++ EMEIPE YI+SLPKNGRASLGD +Y++ITV+FFDPDQFLSSMD++SEHKI+DLK+R
Sbjct: 301 QVLFEMEIPESYIDSLPKNGRASLGDQMYKNITVDFFDPDQFLSSMDMSSEHKIVDLKNR 360

Query: 362 IEASIVIWRRKMNQKDGKSS--WGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDI 421
           IEASI+IW+RKM  KD KSS  W S VSLEKRE+FEERAETILLILK R+PGI QSSLDI
Sbjct: 361 IEASIIIWKRKMVYKDNKSSAPWASGVSLEKREVFEERAETILLILKQRYPGISQSSLDI 420

Query: 422 SKIQFNRDIGHAVLESYSRILESLAFTVMSRIEDVLHADGLTQNPTQTRRKPTSETPPEK 481
           SKIQFN D+G AVLESYSRILESLA+TV+SRI+DVL AD                    +
Sbjct: 421 SKIQFNEDVGQAVLESYSRILESLAYTVLSRIDDVLEAD--------------------R 480

Query: 482 SPHKRD---EAAPETPV-SMTLLDFMGWGQEQ---NEAESKKDSFGNSDDLYGDSDLKQA 541
           + +KR+   EA  ET V SMTL DFMGW  +Q    E ESKK       DL  D  +K+ 
Sbjct: 481 AGNKRNTPLEAEEETLVGSMTLSDFMGWDFDQAANAELESKK-------DLPDDPLIKE- 515

Query: 542 NKLGNIVTNKKVSYVENLSAVRSPTSRH 557
            KL ++VT KK SY+E L  V+SPT+RH
Sbjct: 541 -KL-SVVTTKKTSYLETLGGVKSPTARH 515

BLAST of Spg015131 vs. TAIR 10
Match: AT1G52240.1 (RHO guanyl-nucleotide exchange factor 11 )

HSP 1 Score: 577.8 bits (1488), Expect = 9.4e-165
Identity = 331/543 (60.96%), Postives = 394/543 (72.56%), Query Frame = 0

Query: 19  FHFRGNQENAGRHAKSLSMESASMF---DSLLEEDLSSRSQGSTPVHNNSAEKQQSGLPL 78
           F+   N+ +A RH KS S + A      D+L ++D       S P    S ++    +  
Sbjct: 82  FNDNMNENSASRHVKSWSSDCAMRMDGSDNLDDDDNDMMMFRSQPGKCGSVDRPSLPIGG 141

Query: 79  APSNRPEKALKVKLSSRDDDPATEKEARDKIASEMEQIKERFAKLLLGEDMSGGGKGVSS 138
              NR +K  +V  SS D       EA   + + MEQ+KE+F+KLLLGEDMSGGGKGVSS
Sbjct: 142 VTPNRNDKLPRV--SSSD-----SMEALIILQAAMEQMKEKFSKLLLGEDMSGGGKGVSS 201

Query: 139 ALALSNAITNLAASVFGEQWRLEPMSEERKARWRKEIDLLLSVTDYIVEFVPSQQKSKDG 198
           ALALSNAITNLAAS FGEQ RLEPM+ +RK RWR+EI  L+SV DYIVEF P+QQ +KDG
Sbjct: 202 ALALSNAITNLAASAFGEQRRLEPMAVDRKTRWRREIGWLISVADYIVEFAPTQQTNKDG 261

Query: 199 TNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEFYYVSRDADDSEKGNNKRKD 258
           T+ME+M TRQR DL  NIPAL+KLDAML+DCLD FKDQ+EFYYV +D+ DS      R D
Sbjct: 262 TSMEVMSTRQRTDLLCNIPALKKLDAMLLDCLDKFKDQDEFYYVKKDSPDS---CETRND 321

Query: 259 DKWWLPTAKVPPNGLSEMSRKFIQHQKDCVNQVLKAAMAINAQIISEMEIPEDYIESLPK 318
           +KWWLP  KVPPNGLSE+SR+F+Q QK+CVNQVLKAAMAINAQ++SEMEIPE Y+ESLPK
Sbjct: 322 EKWWLPAVKVPPNGLSEISRRFLQSQKECVNQVLKAAMAINAQVLSEMEIPESYLESLPK 381

Query: 319 NGRASLGDSIYRSITVEFFDPDQFLSSMDLTSEHKILDLKDRIEASIVIWRRKMNQKDGK 378
           NGRASLGD IYR ITVE FD DQFL  MDL+SEHKILDLK+RIEASIVIW+RKM QKD K
Sbjct: 382 NGRASLGDVIYRMITVEMFDADQFLIEMDLSSEHKILDLKNRIEASIVIWKRKMVQKDTK 441

Query: 379 SSWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDISKIQFNRDIGHAVLESYSRI 438
           S WGS VS+EKRE FEERAETILL+LK  FPGI QSSLDISKIQFNRD+G A+LESYSR+
Sbjct: 442 SPWGSTVSIEKREQFEERAETILLLLKQGFPGISQSSLDISKIQFNRDVGLAILESYSRV 501

Query: 439 LESLAFTVMSRIEDVLHADGLTQNPTQTRRKPTSETPPEKSPHKRDEAA-PETPVSMTLL 498
           LESLA TVMSRIEDVL+AD LTQ PT         +  E    + +  +  E   S TL 
Sbjct: 502 LESLAHTVMSRIEDVLYADQLTQEPTNNAPSKNRYSLKENEKLREERLSFTEDMASGTLS 561

Query: 499 DFMGWGQEQNEAESKKDSFGNSDDLYGDSDLKQANKLGNIVT-NKKVSYVENLSAVRSPT 557
           D M WG + N  E KK+SF      +GD +    +K+  I+T NKK SY++NL A+RSPT
Sbjct: 562 DVMQWGNKNN--EMKKESF------FGDREKPLLSKVTGIMTNNKKSSYLDNLGAMRSPT 606

BLAST of Spg015131 vs. TAIR 10
Match: AT3G24620.1 (RHO guanyl-nucleotide exchange factor 8 )

HSP 1 Score: 557.4 bits (1435), Expect = 1.3e-158
Identity = 316/536 (58.96%), Postives = 387/536 (72.20%), Query Frame = 0

Query: 33  KSLSMESASMFDSLLEEDLSSRSQGST-PVHNNSAEKQQSGLPLAPSNRPEKALKVKLSS 92
           + LS   +  F  + +   + + Q  T  V N  +   +S  P + ++       V+ S 
Sbjct: 7   RGLSASKSFNFKRMFDSSSTKQQQSQTIVVENGDSHIVESNTPESQNSDSFVESPVESSL 66

Query: 93  RDDDPATEKEAR-DKIASEMEQIKERFAKLLLGEDMSGGGKGVSSALALSNAITNLAASV 152
               P T    R ++  ++ME +K+RFAKLLLGEDMSGGGKGVSSALALSNAITNLAAS+
Sbjct: 67  PMISPLTRPGKRSERQQADMEMMKDRFAKLLLGEDMSGGGKGVSSALALSNAITNLAASI 126

Query: 153 FGEQWRLEPMSEERKARWRKEIDLLLSVTDYIVEFVPSQQKSKDGTNMEIMVTRQRNDLH 212
           FGEQ +L+PM ++R+ARW+KEID LLSVTD+IVEFVPSQQ SKDG   EIMVTRQR DL 
Sbjct: 127 FGEQTKLQPMPQDRQARWKKEIDWLLSVTDHIVEFVPSQQTSKDGVCTEIMVTRQRGDLL 186

Query: 213 LNIPALRKLDAMLIDCLDNFKDQNEFYYVSRDADDSEKGNNKRKDDKWWLPTAKVPPNGL 272
           +NIPALRKLDAMLID LDNF+  NEF+YVSRD+++ ++  N R +DKWWLP  KVPP GL
Sbjct: 187 MNIPALRKLDAMLIDTLDNFRGHNEFWYVSRDSEEGQQARNDRTNDKWWLPPVKVPPGGL 246

Query: 273 SEMSRKFIQHQKDCVNQVLKAAMAINAQIISEMEIPEDYIESLPKNGRASLGDSIYRSIT 332
           SE SR+ +  QKD V QV KAAMAINAQ++SEMEIPE YI+SLPKNGRASLGDSIY+SIT
Sbjct: 247 SEPSRRMLYFQKDSVTQVQKAAMAINAQVLSEMEIPESYIDSLPKNGRASLGDSIYKSIT 306

Query: 333 VEFFDPDQFLSSMDLTSEHKILDLKDRIEASIVIWRRKMNQKDGKSSWGSAVSLEKRELF 392
            E+FDP+QFL+ +D+++EHK+LDLK+RIEAS+VIW+RK++ KD KSSWGSAVSLEKRELF
Sbjct: 307 EEWFDPEQFLAMLDMSTEHKVLDLKNRIEASVVIWKRKLHTKDTKSSWGSAVSLEKRELF 366

Query: 393 EERAETILLILKHRFPGIPQSSLDISKIQFNRDIGHAVLESYSRILESLAFTVMSRIEDV 452
           EERAETIL++LK +FPG+PQSSLDISKIQFN+D+G AVLESYSRILESLA+TVMSRIEDV
Sbjct: 367 EERAETILVLLKQKFPGLPQSSLDISKIQFNKDVGQAVLESYSRILESLAYTVMSRIEDV 426

Query: 453 LHADGLTQNPTQTRRKP-----TSETPPEK--SPHKRDEAAPETPVSMTLLDFMGWGQEQ 512
           L+ D L    T    +      T+ET  E   S +  +EA    P S TLLDFMGW    
Sbjct: 427 LYTDTLALKQTLLAEETSDGGRTTETDSESAGSSNSGEEAEKHDPHSKTLLDFMGW---- 486

Query: 513 NEAESKKDSFGNSDDLYGDSDLKQANKLGNIVTNKKVSY---VENLSAVRSPTSRH 557
           N+  SK           GD   K  N     +T KK+SY   +ENL+  RSP  RH
Sbjct: 487 NDNSSKG----------GDKPTKSPN-----LTPKKLSYLEKLENLNGFRSPKDRH 523

BLAST of Spg015131 vs. TAIR 10
Match: AT4G13240.1 (RHO guanyl-nucleotide exchange factor 9 )

HSP 1 Score: 542.3 bits (1396), Expect = 4.4e-154
Identity = 300/514 (58.37%), Postives = 372/514 (72.37%), Query Frame = 0

Query: 63  NNSAEKQQSGLPLAPSNRPEKALKVKL------SSRDDDPATEKEARDKIASEMEQIKER 122
           ++S  K+Q    LA +  PE   +  L      +SR          +DK  SE E +K+R
Sbjct: 22  DSSPGKEQQQPHLAETTMPESQTQDSLGGSPVETSRPMTSRLISRRQDKQQSETEMMKDR 81

Query: 123 FAKLLLGEDMSGGGKGVSSALALSNAITNLAASVFGEQWRLEPMSEERKARWRKEIDLLL 182
           F KLLLGEDMSGGGKGVSSALALSNAITNLAAS+FGEQ +L+PM+ +R+ARW+KEID LL
Sbjct: 82  FTKLLLGEDMSGGGKGVSSALALSNAITNLAASIFGEQTKLQPMAPDRRARWKKEIDWLL 141

Query: 183 SVTDYIVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPALRKLDAMLIDCLDNFKDQNEF 242
           SVTD+IVEFVPSQQ SK+G   EIMVTRQR DL +NIPALRKLDAMLID LDNF+  NEF
Sbjct: 142 SVTDHIVEFVPSQQISKEGVCTEIMVTRQRGDLLMNIPALRKLDAMLIDTLDNFRGHNEF 201

Query: 243 YYVSRDADDSEKGNNKRKDDKWWLPTAKVPPNGLSEMSRKFIQHQKDCVNQVLKAAMAIN 302
           +YVSRD+++ ++  N+R  DKWWLP  KVPPNGLSE +R+ +  QKD V+QV KAAMAIN
Sbjct: 202 WYVSRDSEEGKQARNERTKDKWWLPPVKVPPNGLSESARRMLHFQKDSVSQVQKAAMAIN 261

Query: 303 AQIISEMEIPEDYIESLPKNGRASLGDSIYRSITVEFFDPDQFLSSMDLTSEHKILDLKD 362
           AQ++SEM IP+ YIESLPKNGR SLGDS+Y+SIT E+FDP+QFLS++DL++EHK+LD+K+
Sbjct: 262 AQVLSEMAIPDSYIESLPKNGRVSLGDSLYKSITEEWFDPEQFLSTLDLSTEHKVLDVKN 321

Query: 363 RIEASIVIWRRKMNQKDGKSSWGSAVSLEKRELFEERAETILLILKHRFPGIPQSSLDIS 422
           RIEASIVIW+RK++ KD KSSWGSAVSLEKRELFEERAETIL++LK +FPG+PQSSLDIS
Sbjct: 322 RIEASIVIWKRKLHLKDNKSSWGSAVSLEKRELFEERAETILVLLKQKFPGLPQSSLDIS 381

Query: 423 KIQFNRDIGHAVLESYSRILESLAFTVMSRIEDVLHADGL-----TQNPTQTRRKPTSET 482
           KIQ+N+D+GHAVLESYSRILESL +T MSRI+DVL+AD L     T   T    K  +ET
Sbjct: 382 KIQYNKDVGHAVLESYSRILESLGYTEMSRIDDVLYADSLARKQCTGEETSDGGKIATET 441

Query: 483 PPEK------SPHKRDEAAPETPVSMTLLDFMGWGQEQNEAESKKDSFGNSDDLYGDSDL 542
                     S  + ++   +     TLLDF+GW    ++ +S+K               
Sbjct: 442 DSASAGSSNYSGEEIEKLESQNSSKTTLLDFIGWSDNSSKGQSEKPPKSPR--------- 501

Query: 543 KQANKLGNIVTNKKVSY---VENLSAVRSPTSRH 557
                    +T KK+SY   +ENL+  RSP  RH
Sbjct: 502 ---------MTPKKLSYLEKLENLNGFRSPKDRH 517

BLAST of Spg015131 vs. TAIR 10
Match: AT5G19560.1 (ROP uanine nucleotide exchange factor 10 )

HSP 1 Score: 491.5 bits (1264), Expect = 8.9e-139
Identity = 264/466 (56.65%), Postives = 347/466 (74.46%), Query Frame = 0

Query: 96  PATEKEARDKIASEMEQIKERFAKLLLGEDMSGGGKGVSSALALSNAITNLAASVFGEQW 155
           P+T +  +++  S+ME +KERFAKLLLGEDMSGGG G +SALALSNAIT LA S+FGEQ 
Sbjct: 32  PSTRRGKQNR-RSDMEVMKERFAKLLLGEDMSGGGTGETSALALSNAITKLADSMFGEQM 91

Query: 156 RLEPMSEERKARWRKEIDLLLSVTDYIVEFVPSQQKSKDGTNMEIMVTRQRNDLHLNIPA 215
           +L+PM  E K  WRKE+  LLSV D+IV+FVPS+Q  K+G   EIMVT+QR+DL  NIPA
Sbjct: 92  KLQPMYPETKENWRKEMGWLLSVIDHIVQFVPSRQMGKNGQFTEIMVTKQRDDLLTNIPA 151

Query: 216 LRKLDAMLIDCLDNFKDQNEFYYVSRDADDSEKGNNKRKDDKWWLPTAKVPPNGLSEMSR 275
           LRKLD++L++ LDNFKDQ +F+YV RD +D++   + R+D+ WWLP  KVP +GLSE SR
Sbjct: 152 LRKLDSVLLETLDNFKDQKDFWYVPRDMEDADHNGDWRRDENWWLPVVKVPTDGLSEESR 211

Query: 276 KFIQHQKDCVNQVLKAAMAINAQIISEMEIPEDYIESLPKNGRASLGDSIYRSITVEFFD 335
           +++Q+QKD V QVLKAA AINA ++SEM +PE+YI+SLPKNG+ SLGD +Y+SIT E FD
Sbjct: 212 RWLQNQKDSVAQVLKAATAINAHVLSEMHVPENYIDSLPKNGKTSLGDFLYKSITEESFD 271

Query: 336 PDQFLSSMDLTSEHKILDLKDRIEASIVIWRRKM--NQKDGKSSWGSAVSLEKRELFEER 395
           PD F+S +DL++EHK+LDLK+RIEAS+VIW+RKM   +KDGKS WGS VSLEKRELFE R
Sbjct: 272 PDYFVSFLDLSTEHKVLDLKNRIEASMVIWKRKMCQKEKDGKSQWGSTVSLEKRELFEVR 331

Query: 396 AETILLILKHRFPGIPQSSLDISKIQFNRDIGHAVLESYSRILESLAFTVMSRIEDVLHA 455
           AETIL++LK +FPGIPQSSL++SKI+ N+D+G A+LESYSR+LESLA  +MSRIEDVL A
Sbjct: 332 AETILVMLKQQFPGIPQSSLEVSKIKNNKDVGQAILESYSRVLESLASKIMSRIEDVLEA 391

Query: 456 DGLTQNPTQTRRKPTSETPPEKSPHKRDE-AAPETPVSMTLLDFMGWGQEQNEAESKKDS 515
           D L Q       +  SE+  E    + ++  A ETP S  L DF+GW   +  +++KK S
Sbjct: 392 DRLVQRQLMGEAETRSESEAESEYEETEKVVAAETPNSRKLSDFIGW---RLSSDTKKHS 451

Query: 516 FGNSDDLYGDSDLKQANKL--GNIVTNKKVSYVENLSAVRSPTSRH 557
             +  + +   + ++   +        KK SY+  L  +RSP+ RH
Sbjct: 452 SMSDIEFFHKVEQEKEKPMMKSPRALPKKFSYLAKLENMRSPSDRH 493

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038899805.15.2e-27489.25rop guanine nucleotide exchange factor 12-like [Benincasa hispida][more]
KAA0059309.15.4e-27189.07rop guanine nucleotide exchange factor 12-like [Cucumis melo var. makuwa] >TYK04... [more]
XP_008462168.26.0e-27088.71PREDICTED: LOW QUALITY PROTEIN: rop guanine nucleotide exchange factor 12-like [... [more]
XP_011659571.12.3e-26688.17rop guanine nucleotide exchange factor 12 [Cucumis sativus][more]
XP_023514662.14.7e-25985.46rop guanine nucleotide exchange factor 12-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9CA892.5e-17061.97Rop guanine nucleotide exchange factor 12 OS=Arabidopsis thaliana OX=3702 GN=ROP... [more]
Q9M8111.1e-16861.14Rop guanine nucleotide exchange factor 11 OS=Arabidopsis thaliana OX=3702 GN=ROP... [more]
Q9LV401.9e-15758.96Rho guanine nucleotide exchange factor 8 OS=Arabidopsis thaliana OX=3702 GN=ROPG... [more]
Q9SVQ36.2e-15358.37Rop guanine nucleotide exchange factor 9 OS=Arabidopsis thaliana OX=3702 GN=ROPG... [more]
Q1KS661.3e-13756.65Rop guanine nucleotide exchange factor 10 OS=Arabidopsis thaliana OX=3702 GN=ROP... [more]
Match NameE-valueIdentityDescription
A0A5D3C0X62.6e-27189.07Rop guanine nucleotide exchange factor 12-like OS=Cucumis melo var. makuwa OX=11... [more]
A0A1S3CGA02.9e-27088.71LOW QUALITY PROTEIN: rop guanine nucleotide exchange factor 12-like OS=Cucumis m... [more]
A0A0A0K7D81.1e-26688.17PRONE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G446980 PE=4 ... [more]
A0A6J1HL163.0e-25985.28rop guanine nucleotide exchange factor 12-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1KI317.4e-25885.10rop guanine nucleotide exchange factor 12-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
Match NameE-valueIdentityDescription
AT1G79860.11.8e-17161.97RHO guanyl-nucleotide exchange factor 12 [more]
AT1G52240.19.4e-16560.96RHO guanyl-nucleotide exchange factor 11 [more]
AT3G24620.11.3e-15858.96RHO guanyl-nucleotide exchange factor 8 [more]
AT4G13240.14.4e-15458.37RHO guanyl-nucleotide exchange factor 9 [more]
AT5G19560.18.9e-13956.65ROP uanine nucleotide exchange factor 10 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.20.58.1310PRONE domain, subdomain 2coord: 304..420
e-value: 3.3E-151
score: 504.7
NoneNo IPR availableGENE3D1.20.58.2010PRONE domain, subdomain 1coord: 114..455
e-value: 3.3E-151
score: 504.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 47..76
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 454..487
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 468..485
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 500..523
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 47..104
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 85..104
NoneNo IPR availablePANTHERPTHR33101:SF10ROP GUANINE NUCLEOTIDE EXCHANGE FACTOR 12coord: 15..556
IPR005512PRONE domainPFAMPF03759PRONEcoord: 108..458
e-value: 1.5E-176
score: 586.6
IPR005512PRONE domainPROSITEPS51334PRONEcoord: 100..464
score: 117.090469
IPR038937Rop guanine nucleotide exchange factorPANTHERPTHR33101ROP GUANINE NUCLEOTIDE EXCHANGE FACTOR 1coord: 15..556

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg015131.1Spg015131.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0050790 regulation of catalytic activity
molecular_function GO:0005085 guanyl-nucleotide exchange factor activity