Spg015096 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg015096
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptiongibberellin 20-oxidase-like protein
Locationscaffold3: 42070826 .. 42072976 (-)
RNA-Seq ExpressionSpg015096
SyntenySpg015096
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGGAAGAACGGACGGAGAGATCGAGTGAGGAAGTTCGAGTTCCAGTGATCGACATTCATTCAGAGAGCCTGAGCGAATCGAGTGTTGAAGCTCTGAGAGAAGCATGCAGGGAATGGGGATTCTTCTACATCAGAAACCATGGCGTTTCGAAGGAGTTTTACCGGAAACTCGCCGCCGGCGCCAACGAACTCTTCTCTTTTCCCGAAGAGACGAAGCGCAAAATGAAACTGGGAATTGAATCCTACACGCCGAGATTTGTGGTGTCGCCCTTCGTGGAGAGCTTCAAGGTTATGGGGCCTCACTTCTCTGCCTCTGCTGCTGCGCAAGGTTTCACTGAGTCGTTATTCGGCCACCACGCTGCTGAATTTAGGTCTACTTTCTTCCCCCTTCTCTTTAATTTTACGGTGTCGCATTCGTCGATGATCTTCGGTTACTATGATGTGGCTGTCTCTTGAAAACGACGATGTGCGAATGTTAAATATGCGTGCCTTAATAATCATTGAGTAGATGTTATTTGAAATAGTCTCTTTTTTTTTTAGTTTCTCTTTAATACATCAAATGATATAAACATGTGTTGCTAGAAATTGTTCAAGGAAGTCATATCATCATGTGTCCGACCATTTTATATCATTGTATGTGTATGGTTGCTAATTAAGGGCAAATTATTAGGTGCTTCCGTGCAATGAGACTCCCTCTTTGAAACCATCAGATGTATATCTTCAAATTTGTCTGTGATAACTATTGGATATAAAATCGGATAGTTTAACAATTTATCTCTCTTACATCTAATGGTGAAAAATTATCCGTCTTATTGCACCTAGAGCACCTAATAATTTTCCGTTGCTAAGTGACAACCACATTATTATTATTATTATTATTATTTTGAGAAGAGAGAGACAGCCACATTATTGCAGCCACTTATAATTTTTTTTAATTTGCATGCCTTCTCCTTTTCAAGGTGCTTGTTTTTTGTAGGTTTAAAAATTTGAAGTTATTGTGCATGTTTTTTGTATCCAATAATTATCAAAGAATATATGGATAATATATATTCAACGGTTGCTAAGAGACAACCACGTTATTGTGGTGGCTTAATATATTTTCTTTTAATATTCTTCTTTGCTAACTTTGTCAAATTTAAACATAGGTTAAGTAACAAGTTTAGTTGGATTGTAGCTTCTAAAAAAAATAGAGCTTCTATTTTTCAAAAAAAAAAAAAAAAAAATAGAGCTTCTAAACTTAAAAAAAAAATGTCTAAATAACAGGTCCTTCAAAAAATGTCAAATAGAACAGAAACTCTATTATATTGGACAAAATATCTTAGAAGTTTAAAAACTAAACTTGTAATTCCTATCAAATTATTGAAATTTAATGAAAACTTTTTAAAACACAAAAATATTGAATAGTCAACTTGAAAATTTGGGACCAACAAGTTTAACCTTTTGTGCAATTATTTGCAAACCAATCTCTTCATATTTTAATTATGAGATTGTTCTATATCATCTAGGCACTAATCTCTTTGTAAGTTTCATTTTAATTTAATTTGCAGCACTCTACTAGACGAATATGGAAGCAAAATGAAAGAACTATCAAAAAGAATAATACAGCTATTACTAAAGATCTTGGGAGATAATTTTGTAAACAAATTCTATGATACCGAATTCAGTAACTGCAATGGCTACCTAAGGATAAACGGCTGCTCTCCTCAAGACTTGAACGACGAAGAAGAAGTCGTAGTGTTCGAGAAGCACACCGACATCAGCTGCGTGACGATTTTGTTCCAAGACGAGGTCGGGGGGCTACAGATGAGATCGAAGCGAGGAGAGTGGATCGACGTAAGGCCGAGCGAGGATGCTGATGATCTTGTGGTGAACATTGGGGATTTTATGCAGGCTTGGAGCAATGAGAGGCTGAGATCAGCAGAACATAGAGTTGTTTTGAGGAAAAATAATGTGAAGAGATTCTCTGTGGCTTTCTTTTTGATATATGGAGATGATCATAAAGAAATATATGCACCAAGTGAGGTTGTTGGAGAAGGGAATGCAAGGCTTTATTGCCCATTTTCAACTGAAGAATATAGGGTTTTTAGAGAAAAGAACTATGAGAAAATTGGAGCCACACTCAAAGATTTTGCTGGAATTGTAGGGTGA

mRNA sequence

ATGTCGGAAGAACGGACGGAGAGATCGAGTGAGGAAGTTCGAGTTCCAGTGATCGACATTCATTCAGAGAGCCTGAGCGAATCGAGTGTTGAAGCTCTGAGAGAAGCATGCAGGGAATGGGGATTCTTCTACATCAGAAACCATGGCGTTTCGAAGGAGTTTTACCGGAAACTCGCCGCCGGCGCCAACGAACTCTTCTCTTTTCCCGAAGAGACGAAGCGCAAAATGAAACTGGGAATTGAATCCTACACGCCGAGATTTGTGGTGTCGCCCTTCGTGGAGAGCTTCAAGGTTATGGGGCCTCACTTCTCTGCCTCTGCTGCTGCGCAAGGTTTCACTGAGTCGTTATTCGGCCACCACGCTGCTGAATTTAGCACTCTACTAGACGAATATGGAAGCAAAATGAAAGAACTATCAAAAAGAATAATACAGCTATTACTAAAGATCTTGGGAGATAATTTTGTAAACAAATTCTATGATACCGAATTCAGTAACTGCAATGGCTACCTAAGGATAAACGGCTGCTCTCCTCAAGACTTGAACGACGAAGAAGAAGTCGTAGTGTTCGAGAAGCACACCGACATCAGCTGCGTGACGATTTTGTTCCAAGACGAGGTCGGGGGGCTACAGATGAGATCGAAGCGAGGAGAGTGGATCGACGTAAGGCCGAGCGAGGATGCTGATGATCTTGTGGTGAACATTGGGGATTTTATGCAGGCTTGGAGCAATGAGAGGCTGAGATCAGCAGAACATAGAGTTGTTTTGAGGAAAAATAATGTGAAGAGATTCTCTGTGGCTTTCTTTTTGATATATGGAGATGATCATAAAGAAATATATGCACCAAGTGAGGTTGTTGGAGAAGGGAATGCAAGGCTTTATTGCCCATTTTCAACTGAAGAATATAGGGTTTTTAGAGAAAAGAACTATGAGAAAATTGGAGCCACACTCAAAGATTTTGCTGGAATTGTAGGGTGA

Coding sequence (CDS)

ATGTCGGAAGAACGGACGGAGAGATCGAGTGAGGAAGTTCGAGTTCCAGTGATCGACATTCATTCAGAGAGCCTGAGCGAATCGAGTGTTGAAGCTCTGAGAGAAGCATGCAGGGAATGGGGATTCTTCTACATCAGAAACCATGGCGTTTCGAAGGAGTTTTACCGGAAACTCGCCGCCGGCGCCAACGAACTCTTCTCTTTTCCCGAAGAGACGAAGCGCAAAATGAAACTGGGAATTGAATCCTACACGCCGAGATTTGTGGTGTCGCCCTTCGTGGAGAGCTTCAAGGTTATGGGGCCTCACTTCTCTGCCTCTGCTGCTGCGCAAGGTTTCACTGAGTCGTTATTCGGCCACCACGCTGCTGAATTTAGCACTCTACTAGACGAATATGGAAGCAAAATGAAAGAACTATCAAAAAGAATAATACAGCTATTACTAAAGATCTTGGGAGATAATTTTGTAAACAAATTCTATGATACCGAATTCAGTAACTGCAATGGCTACCTAAGGATAAACGGCTGCTCTCCTCAAGACTTGAACGACGAAGAAGAAGTCGTAGTGTTCGAGAAGCACACCGACATCAGCTGCGTGACGATTTTGTTCCAAGACGAGGTCGGGGGGCTACAGATGAGATCGAAGCGAGGAGAGTGGATCGACGTAAGGCCGAGCGAGGATGCTGATGATCTTGTGGTGAACATTGGGGATTTTATGCAGGCTTGGAGCAATGAGAGGCTGAGATCAGCAGAACATAGAGTTGTTTTGAGGAAAAATAATGTGAAGAGATTCTCTGTGGCTTTCTTTTTGATATATGGAGATGATCATAAAGAAATATATGCACCAAGTGAGGTTGTTGGAGAAGGGAATGCAAGGCTTTATTGCCCATTTTCAACTGAAGAATATAGGGTTTTTAGAGAAAAGAACTATGAGAAAATTGGAGCCACACTCAAAGATTTTGCTGGAATTGTAGGGTGA

Protein sequence

MSEERTERSSEEVRVPVIDIHSESLSESSVEALREACREWGFFYIRNHGVSKEFYRKLAAGANELFSFPEETKRKMKLGIESYTPRFVVSPFVESFKVMGPHFSASAAAQGFTESLFGHHAAEFSTLLDEYGSKMKELSKRIIQLLLKILGDNFVNKFYDTEFSNCNGYLRINGCSPQDLNDEEEVVVFEKHTDISCVTILFQDEVGGLQMRSKRGEWIDVRPSEDADDLVVNIGDFMQAWSNERLRSAEHRVVLRKNNVKRFSVAFFLIYGDDHKEIYAPSEVVGEGNARLYCPFSTEEYRVFREKNYEKIGATLKDFAGIVG
Homology
[Close]
BLAST of Spg015096 vs. NCBI nr
Match: XP_038899419.1 (gibberellin 20-oxidase-like protein [Benincasa hispida])

HSP 1 Score: 457.2 bits (1175), Expect = 1.2e-124
Identity = 222/311 (71.38%), Postives = 266/311 (85.53%), Query Frame = 0 

Query: 15  VPVIDIHSESLSESSVEALREACREWGFFYIRNHGVSKEFYRKLAAGAN-ELFSFPEETK 74
           +PV+DI+S S S S + +L +AC+EWGFF+IRNHG+ KEFY KL + A+  LFSFP+ETK
Sbjct: 10  IPVMDIYSHSFSNSDISSLSKACKEWGFFFIRNHGIPKEFYGKLISDADRRLFSFPDETK 69

Query: 75  RKMKLGIESYTPRFVVSPFVESFKVMGPHFSASAAAQGFTESLFGHHAAEFSTLLDEYGS 134
           RKMK+G+ SYTPRFVVSP +ESFKV+GP+FSASAAA GFT+S+FG  A EF  LLDEYGS
Sbjct: 70  RKMKVGVSSYTPRFVVSPSMESFKVLGPNFSASAAAHGFTDSIFGRQATEFRNLLDEYGS 129

Query: 135 KMKELSKRIIQLLLKILGDNFVNKFYDTEFSNCNGYLRINGCSPQDLNDEEEVVVFEKHT 194
           KM+E+SKR++QLLLK LG++  NKFY++EF NC+GYLRIN  + QDLNDEEEVV F+KHT
Sbjct: 130 KMEEVSKRVVQLLLKTLGEDLENKFYESEFGNCHGYLRINSYTLQDLNDEEEVVGFKKHT 189

Query: 195 DISCVTILFQDEVGGLQMRSKRGEWIDVRPSEDADDLVVNIGDFMQAWSNERLRSAEHRV 254
           D+S VTILFQD+VGGLQMRSK GEW DV+PS+D  +L+VNIGDFM+AWSN ++RSAEHRV
Sbjct: 190 DLSYVTILFQDDVGGLQMRSKGGEWFDVKPSKDG-NLLVNIGDFMEAWSNGKVRSAEHRV 249

Query: 255 VLRK--NNVKRFSVAFFLIYGDDHKEIYAPSEVVGEGNARLYCPFSTEEYRVFREKNYEK 314
           VLRK  N  KRFSVAFFL Y D  +EIYAP+EVVGEG +RLY PFST+EYR+FREKNYEK
Sbjct: 250 VLRKKINVKKRFSVAFFLTYEDGDREIYAPTEVVGEGKSRLYLPFSTQEYRLFREKNYEK 309

Query: 315 IGATLKDFAGI 323
           I ATLKDFAGI
Sbjct: 310 IDATLKDFAGI 319

[Close]
BLAST of Spg015096 vs. NCBI nr
Match: XP_022953358.1 (gibberellin 20-oxidase-like protein [Cucurbita moschata])

HSP 1 Score: 451.1 bits (1159), Expect = 8.3e-123
Identity = 218/308 (70.78%), Postives = 259/308 (84.09%), Query Frame = 0 

Query: 15  VPVIDIHSESLSESSVEALREACREWGFFYIRNHGVSKEFYRKLAAGANELFSFPEETKR 74
           VPVID+  +SL+ES+  +L   C E GFF+IRNHGVSKEFY KL +GA+ELFS PEETK+
Sbjct: 6   VPVIDLSCQSLTESTASSLLRTCNELGFFFIRNHGVSKEFYEKLISGADELFSSPEETKQ 65

Query: 75  KMKLGIESYTPRFVVSPFVESFKVMGPHFSASAAAQGFTESLFGHHAAEFSTLLDEYGSK 134
           ++K+G+ SYTPRFVVSPF ESF+V GP+FSAS AAQGFT+SLFGHHA EFS LLDEYGSK
Sbjct: 66  QLKMGVSSYTPRFVVSPFFESFRVSGPNFSASTAAQGFTQSLFGHHATEFSNLLDEYGSK 125

Query: 135 MKELSKRIIQLLLKILGDNFVNKFYDTEFSNCNGYLRINGCSPQDLNDEEEVVVFEKHTD 194
           MKE+SKR+++LLL ILGDNF NKFYD EFSNCNGYLRING +P D+N EEEV VF+KHTD
Sbjct: 126 MKEVSKRVVKLLLNILGDNFGNKFYDPEFSNCNGYLRINGFTPLDMNAEEEVEVFKKHTD 185

Query: 195 ISCVTILFQDEVGGLQMRSKRGEWIDVRPSEDADDLVVNIGDFMQAWSNERLRSAEHRVV 254
           +S VTI+F +++ GLQMRSK G+WIDVR SED D LVVNIGD+M+AWSN RLRSA HRVV
Sbjct: 186 LSYVTIVFPNDMRGLQMRSKGGDWIDVRSSED-DILVVNIGDYMEAWSNGRLRSAVHRVV 245

Query: 255 LRKNNVKRFSVAFFLIYGDDHKEIYAPSEVVGEGNARLYCPFSTEEYRVFREKNYEKIGA 314
           LRKN  KRFS+AF     D+ +EIYAP+EVVGEG +RLY  FS ++Y+ FRE NYEK+GA
Sbjct: 246 LRKNITKRFSIAFLSTPEDESREIYAPNEVVGEGKSRLYRSFSIQQYKHFRENNYEKVGA 305

Query: 315 TLKDFAGI 323
           T+KDF GI
Sbjct: 306 TIKDFVGI 312

[Close]
BLAST of Spg015096 vs. NCBI nr
Match: XP_022991455.1 (gibberellin 20-oxidase-like protein [Cucurbita maxima])

HSP 1 Score: 448.0 bits (1151), Expect = 7.1e-122
Identity = 215/308 (69.81%), Postives = 260/308 (84.42%), Query Frame = 0 

Query: 15  VPVIDIHSESLSESSVEALREACREWGFFYIRNHGVSKEFYRKLAAGANELFSFPEETKR 74
           VPVID+  +SL+ES+  +L  AC + GFF+IRNHGVSKEFY KL +GA+ELFS PEETK+
Sbjct: 6   VPVIDLGCQSLTESTSSSLLRACNKLGFFFIRNHGVSKEFYEKLISGADELFSSPEETKQ 65

Query: 75  KMKLGIESYTPRFVVSPFVESFKVMGPHFSASAAAQGFTESLFGHHAAEFSTLLDEYGSK 134
           ++K+G+ SYTPRF+VSPF ESF+V GP+FSASAAAQGFT+SLFGHHA EFS LLDEYG K
Sbjct: 66  QLKMGVSSYTPRFLVSPFFESFRVSGPNFSASAAAQGFTQSLFGHHATEFSNLLDEYGRK 125

Query: 135 MKELSKRIIQLLLKILGDNFVNKFYDTEFSNCNGYLRINGCSPQDLNDEEEVVVFEKHTD 194
           MKE+SKR+++LLL +LGDN  NKFYD EFSNCNGYLRING +P D+N EEE+ VF+KHTD
Sbjct: 126 MKEVSKRVVKLLLNVLGDNIENKFYDPEFSNCNGYLRINGFTPLDMNAEEEIEVFKKHTD 185

Query: 195 ISCVTILFQDEVGGLQMRSKRGEWIDVRPSEDADDLVVNIGDFMQAWSNERLRSAEHRVV 254
           +S VTI+FQ+++ GLQMRSK G+WIDVR SED   LVVNIGD+M+AWSN RLRSA HRVV
Sbjct: 186 LSYVTIVFQNDIRGLQMRSKGGDWIDVRLSEDG-ILVVNIGDYMEAWSNGRLRSAVHRVV 245

Query: 255 LRKNNVKRFSVAFFLIYGDDHKEIYAPSEVVGEGNARLYCPFSTEEYRVFREKNYEKIGA 314
           LRKN +KRFS+AF     D+ +EIYAPSEVVGEG +RLY  FS ++Y+ FRE NYEK+GA
Sbjct: 246 LRKNIMKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSVQQYKHFRENNYEKVGA 305

Query: 315 TLKDFAGI 323
           T+KDF GI
Sbjct: 306 TIKDFVGI 312

[Close]
BLAST of Spg015096 vs. NCBI nr
Match: KAG6576056.1 (Gibberellin 20-oxidase-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 444.9 bits (1143), Expect = 6.0e-121
Identity = 215/308 (69.81%), Postives = 257/308 (83.44%), Query Frame = 0 

Query: 15  VPVIDIHSESLSESSVEALREACREWGFFYIRNHGVSKEFYRKLAAGANELFSFPEETKR 74
           VPVID+ S+SL+ES+  +L  AC E GFF+IRNHGVSKEFY KL +GA+ELFS PEETK+
Sbjct: 6   VPVIDLSSQSLTESTASSLLRACNELGFFFIRNHGVSKEFYEKLISGADELFSSPEETKQ 65

Query: 75  KMKLGIESYTPRFVVSPFVESFKVMGPHFSASAAAQGFTESLFGHHAAEFSTLLDEYGSK 134
           ++K+G+ SYTPRFVVSPF ESF+V GP+FS SAA  GFT+SLFGHHA EFS LLDEYGSK
Sbjct: 66  QLKMGVSSYTPRFVVSPFFESFRVSGPNFSTSAATHGFTQSLFGHHATEFSNLLDEYGSK 125

Query: 135 MKELSKRIIQLLLKILGDNFVNKFYDTEFSNCNGYLRINGCSPQDLNDEEEVVVFEKHTD 194
           MKE+SKR+++LLL IL DN  NKFYD EFSNCNGYLRING +P D+N EEEV VF+KHTD
Sbjct: 126 MKEVSKRVVKLLLNILRDNIENKFYDPEFSNCNGYLRINGFTPLDMNAEEEVEVFKKHTD 185

Query: 195 ISCVTILFQDEVGGLQMRSKRGEWIDVRPSEDADDLVVNIGDFMQAWSNERLRSAEHRVV 254
           +S VTI+F +++ GLQMRSK G+WID+R SED D LVVNIGD+M+AWSN RLRSA HRVV
Sbjct: 186 LSYVTIVFPNDMRGLQMRSKGGDWIDIRSSED-DILVVNIGDYMEAWSNGRLRSAVHRVV 245

Query: 255 LRKNNVKRFSVAFFLIYGDDHKEIYAPSEVVGEGNARLYCPFSTEEYRVFREKNYEKIGA 314
           LRKN  KRFS+AF     D+ +EI+APSEVVGEG +RLY  FS ++Y+ FRE NYEK+GA
Sbjct: 246 LRKNITKRFSIAFLSTPEDESREIHAPSEVVGEGKSRLYRSFSIQQYKHFRENNYEKVGA 305

Query: 315 TLKDFAGI 323
           T+KDF GI
Sbjct: 306 TIKDFVGI 312

[Close]
BLAST of Spg015096 vs. NCBI nr
Match: XP_022991453.1 (gibberellin 20-oxidase-like protein isoform X1 [Cucurbita maxima])

HSP 1 Score: 440.7 bits (1132), Expect = 1.1e-119
Identity = 214/308 (69.48%), Postives = 261/308 (84.74%), Query Frame = 0 

Query: 13  VRVPVIDIHSESLSESSVEALREACREWGFFYIRNHGVSKEFYRKLAAGANELFSFPEET 72
           V VPVIDI S+SL+ES+  +L  AC E+GFF+IRNHG+SKE Y+KL +GA++LFS PEET
Sbjct: 4   VEVPVIDISSQSLTESAASSLLRACNEFGFFFIRNHGLSKELYQKLISGADQLFSSPEET 63

Query: 73  KRKMKLGIESYTPRFVVSPFVESFKVMGPHFSASAAAQGFTESLFGHHAAEFSTLLDEYG 132
           K+K+ +G  SY PRFV+SPF ESFKV GP+FSASA AQGFT+SLFGH A EFS LLDEYG
Sbjct: 64  KQKLTIGASSYIPRFVLSPFFESFKVSGPNFSASANAQGFTQSLFGHQATEFSNLLDEYG 123

Query: 133 SKMKELSKRIIQLLLKILGDNFVNKFYDTEFSNCNGYLRINGCSPQDLN-DEEEVVVFEK 192
           SKMKE+SKR+++LLL ILGDN  NKFYD+EFSNCN Y+RING +P+ +N +EEE+ VF+K
Sbjct: 124 SKMKEVSKRVVKLLLNILGDNIENKFYDSEFSNCNAYIRINGFTPRGMNEEEEELEVFKK 183

Query: 193 HTDISCVTILFQDEVGGLQMRSKRGEWIDVRPSEDADDLVVNIGDFMQAWSNERLRSAEH 252
           HTD+S VTI+FQ+++ GLQMR K G+W+DVRPSED ++LVVNIGDFM+AWSN RLRSA H
Sbjct: 184 HTDLSYVTIVFQNDIRGLQMRLKGGDWMDVRPSED-NNLVVNIGDFMEAWSNGRLRSAVH 243

Query: 253 RVVLRKNNVKRFSVAFFLIYGDDHKEIYAPSEVVGEGNARLYCPFSTEEYRVFREKNYEK 312
           RVVLRKNN+KRFS+AFFL   DD KEIYAPSE+VGEG +RLY PFST++YR+F+EKN   
Sbjct: 244 RVVLRKNNMKRFSMAFFLTPKDDDKEIYAPSEIVGEGKSRLYRPFSTQQYRLFKEKNDVN 303

Query: 313 IGATLKDF 320
            G  LKDF
Sbjct: 304 AGYQLKDF 310

[Close]
BLAST of Spg015096 vs. ExPASy Swiss-Prot
Match: F4KBY0 (Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310 PE=2 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 6.5e-70
Identity = 149/329 (45.29%), Postives = 218/329 (66.26%), Query Frame = 0 

Query: 13  VRVPVIDIHSESLSESSVEALREACREWGFFYIRNHGVSKEFYRKLAAGANELFSFPEET 72
           + +PV DI S+ LSESS+ +L++AC+EWGFFY+ NHGVS++ Y+KL   +  +F   +E 
Sbjct: 3   LELPVFDI-SKPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKLRRFSTGVFELEDE- 62

Query: 73  KRKMKLGIESYTPRFVVSPFVESFKVMGPHFSASAAAQGFTESLFGHHAA--EFSTLLDE 132
             KMK+G  +YTPRF+ SPF ES +V GP F ASA +   +   F   A   EFS L+ E
Sbjct: 63  -EKMKMGASNYTPRFIASPFFESLRVSGPDFYASAKS---SVDAFSDQATDEEFSGLMKE 122

Query: 133 YGSKMKELSKRIIQLLLKILGDNFVNKFYDTEFSNCNGYLRINGCS-PQDLNDE----EE 192
           YG KM +L ++I++ +L   GD+  +K+Y++EF NC+GY RIN  + P D  D+    +E
Sbjct: 123 YGEKMTKLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDDHHNGDE 182

Query: 193 VVVFE---KHTDISCVTILFQDEVGGLQMRSKRG-EWIDVRPSEDADDLVVNIGDFMQAW 252
             + E    HTD+SC+TI+ QD++GGLQ+R++ G   +D+ P ++A  LVVN+GD + AW
Sbjct: 183 QDLIEGLGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINPKDEA--LVVNVGDLLHAW 242

Query: 253 SNERLRSAEHRVVLRKNNV--KRFSVAFFLIYGDDHKEIYAPSEVVG--EGNARLYCPFS 312
           +N RLRS++HRV+L++      RFS+AFF  + DD K ++AP EVVG  EG  R++  F 
Sbjct: 243 TNGRLRSSQHRVILKRRGFVGNRFSLAFFWCF-DDGKVVFAPDEVVGGCEG-MRVFRSFK 302

Query: 313 TEEYRVFREKN----YEKIGATLKDFAGI 323
             +Y  FRE N    +EK+G T++DFA I
Sbjct: 303 CGDYLRFRESNEKGKFEKVGDTVEDFARI 321

[Close]
BLAST of Spg015096 vs. ExPASy Swiss-Prot
Match: O49561 (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 SV=2)

HSP 1 Score: 123.6 bits (309), Expect = 4.0e-27
Identity = 96/309 (31.07%), Postives = 147/309 (47.57%), Query Frame = 0 

Query: 11  EEVRVPVIDIH-----SESLSESSVEALREACREWGFFYIRNHGVSKEFYRKLAAGANEL 70
           EEV +PVID+      +E   E   EA+  A REWGFF + NHG+S +   K+      +
Sbjct: 38  EEVELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRV 97

Query: 71  FSFPEETKRKM-KLGIESYTPRFVVSPFVESFKVMGPHFSASAAAQGFTESLFGHHAAEF 130
           F  P + K K  K    SY      +P   S + +     + A     T+          
Sbjct: 98  FREPFDKKSKSEKFSAGSYR---WGTPSATSIRQLS---WSEAFHVPMTDISDNKDFTTL 157

Query: 131 STLLDEYGSKMKELSKRIIQLLLKILGDNFVNKFYDTEFSNCNGYLRINGCSPQDLNDEE 190
           S+ ++++ S+ + L+  + ++L +  G N  + F+         YLR+N   P       
Sbjct: 158 SSTMEKFASESEALAYMLAEVLAEKSGQN--SSFFKENCVRNTCYLRMNRYPP--CPKPS 217

Query: 191 EVVVFEKHTDISCVTILFQDEVGGLQMRSKRGEWIDVRPSEDADDLVVNIGDFMQAWSNE 250
           EV     HTD   +TIL+QD+VGGLQ+  K   WI V+P+  A  L++NIGD  QAWSN 
Sbjct: 218 EVYGLMPHTDSDFLTILYQDQVGGLQL-IKDNRWIAVKPNPKA--LIINIGDLFQAWSNG 277

Query: 251 RLRSAEHRVVLRKNNVKRFSVAFFLIYGDDHKEIYAPSEVVGEGNARLYCPFSTEEYRVF 310
             +S EHR V+    V+RFS A+F+    D       + +    +   Y  FS  E+R  
Sbjct: 278 MYKSVEHR-VMTNPKVERFSTAYFMCPSYD-------AVIECSSDRPAYRNFSFREFRQQ 325

Query: 311 REKNYEKIG 314
            +++ +K G
Sbjct: 338 VQEDVKKFG 325

[Close]
BLAST of Spg015096 vs. ExPASy Swiss-Prot
Match: Q9C899 (Feruloyl CoA ortho-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=F6'H2 PE=1 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 8.9e-27
Identity = 104/323 (32.20%), Postives = 150/323 (46.44%), Query Frame = 0 

Query: 8   RSSEEVRVPVIDIHSESLSESSV-EALREACREWGFFYIRNHGVSKEFYRKLAAGANELF 67
           +   +  +PVIDI   +L E SV +A+ +A  EWGFF + NHGVS E    +    +  F
Sbjct: 55  KEDSDESIPVIDI--SNLDEKSVSKAVCDAAEEWGFFQVINHGVSMEVLENMKTATHRFF 114

Query: 68  SFPEETKRKMKLGIESYTPRFVVSPFVESFKVMGPHFSASAAAQGFTESLFGHHAAEFST 127
             P E KRK       ++    +S  V       PH   +   + +    F   A     
Sbjct: 115 GLPVEEKRK-------FSREKSLSTNVRFGTSFSPHAEKALEWKDYLSLFFVSEAEASQL 174

Query: 128 LLDEYGSKMKEL---SKRIIQLLLKILGDNF-VNKFYDTEFSNCNGYLRINGCSPQDLND 187
             D   S+  E    +K +++ LL+ LG+N  V +   T+ S   G  RIN        +
Sbjct: 175 WPDSCRSETLEYMNETKPLVKKLLRFLGENLNVKELDKTKESFFMGSTRINLNYYPICPN 234

Query: 188 EEEVVVFEKHTDISCVTILFQDEVGGLQMRS-KRGEWIDVRPSEDADDLVVNIGDFMQAW 247
            E  V   +H+D+S +TIL QDE+GGL +RS   G W+ V P   +  LV+NIGD MQ  
Sbjct: 235 PELTVGVGRHSDVSSLTILLQDEIGGLHVRSLTTGRWVHVPPI--SGSLVINIGDAMQIM 294

Query: 248 SNERLRSAEHRVVLRKNNVKRFSVAFFLIYGDDHKEIYAP-SEVVGEGNARLYCPFSTEE 307
           SN R +S EHR VL   +  R SV  F+      + +  P  EV+  G   +Y      +
Sbjct: 295 SNGRYKSVEHR-VLANGSYNRISVPIFV--SPKPESVIGPLLEVIENGEKPVYKDILYTD 354

Query: 308 Y-RVFREKNYEKIGATLKDFAGI 323
           Y + F  K ++  G    DFA I
Sbjct: 355 YVKHFFRKAHD--GKKTIDFANI 361

[Close]
BLAST of Spg015096 vs. ExPASy Swiss-Prot
Match: Q9C6I4 (Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 4.4e-26
Identity = 99/329 (30.09%), Postives = 161/329 (48.94%), Query Frame = 0 

Query: 2   SEERTERSSEE---VRVPVIDIH-----SESLSESSVEALREACREWGFFYIRNHGVSKE 61
           SE  T  S+ +   +++PVID+       E   +  V+ +  A +EWGFF I NHG+ K+
Sbjct: 24  SESNTNTSTIQTSGIKLPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKD 83

Query: 62  FYRKLAAGANELFSFPEETKRKMKLGIESYTPRFVVSPFVESFKVMGPHFSASAAAQGFT 121
            +  +     +LF  P   K + +    S            S++   P  SA++ AQ   
Sbjct: 84  VFEMMLLEEKKLFDQPFSVKVRERFSDLS----------KNSYRWGNP--SATSPAQYSV 143

Query: 122 ESLFGHHAAEFSTLLDEYG-------SKMKELSKRIIQLLLKILGD--NFVNKFYDTEFS 181
              F    +E S + D+         + ++E++ R+ Q++ +ILG   N  +++++  F 
Sbjct: 144 SEAFHIILSEVSRISDDRNNLRTIVETYVQEIA-RVAQMICEILGKQVNVSSEYFENIFE 203

Query: 182 NCNGYLRINGCSPQDLNDEEEVVVFEKHTDISCVTILFQDEVGGLQMRSKRGEWIDVRPS 241
             N +LR+N   P       EV     HTD S +TIL QD++GGL++ +  G+WI V+P 
Sbjct: 204 LENSFLRLNKYHPSVFG--SEVFGLVPHTDTSFLTILSQDQIGGLELEN-NGQWISVKPC 263

Query: 242 EDADDLVVNIGDFMQAWSNERLRSAEHRVVLRKNNVKRFSVAFFLIYGDDHKEIYAPSEV 301
            +A  L VNIGD  QA SN   +S  HRV+    N++R S+AFF+         Y  +E+
Sbjct: 264 LEA--LTVNIGDMFQALSNGVYQSVRHRVI-SPANIERMSIAFFVC-------PYLETEI 323

Query: 302 VGEGNARLYCPFSTEEYRVFREKNYEKIG 314
              G  + Y  FS  EY+   E + ++ G
Sbjct: 324 DCFGYPKKYRRFSFREYKEQSEHDVKETG 326

[Close]
BLAST of Spg015096 vs. ExPASy Swiss-Prot
Match: Q39111 (Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GA20OX2 PE=2 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.7e-25
Identity = 101/331 (30.51%), Postives = 151/331 (45.62%), Query Frame = 0 

Query: 3   EERTERSSEEVRVPVIDIHSESLSESSVEALREACREWGFFYIRNHGVSKEFYRKLAAGA 62
           EE+      E+ VP ID+ S+  +  +   + EAC + GFF + NHGVS+          
Sbjct: 51  EEKPSIDIPELNVPFIDLSSQDSTLEAPRVIAEACTKHGFFLVVNHGVSESLIADAHRLM 110

Query: 63  NELFSFPEETKRK--MKLG-----IESYTPRFVVS-PFVE--SFKVMGPHFSASAAAQGF 122
              F  P   K+K   K G       S+T RF    P+ E  SF+    + S S   Q +
Sbjct: 111 ESFFDMPLAGKQKAQRKPGESCGYASSFTGRFSTKLPWKETLSFQFSNDN-SGSRTVQDY 170

Query: 123 TESLFGHHAAEFSTLLDEYGSKMKELSKRIIQLLLKILGDNFVNKFYDTEFSNCNGYLRI 182
                G    +F  +  +Y   M  LS +I++LL   LG N    ++   F   +  +R+
Sbjct: 171 FSDTLGQEFEQFGKVYQDYCEAMSSLSLKIMELLGLSLGVN--RDYFRGFFEENDSIMRL 230

Query: 183 NGCSPQDLNDEEEVVVFEKHTDISCVTILFQDEVGGLQMRSKRGEWIDVRPSEDADDLVV 242
           N   P    D    +    H D S +TIL QD V GLQ+     +W  +RP+  A   VV
Sbjct: 231 NHYPPCQTPD--LTLGTGPHCDPSSLTILHQDHVNGLQV-FVDNQWQSIRPNPKA--FVV 290

Query: 243 NIGDFMQAWSNERLRSAEHRVVLRKNNVKRFSVAFFLIYGDDHKEIYAPSEVVGEGNARL 302
           NIGD   A SN   +S  HR V+ + + ++ S+AFFL    D K +  PS+++ +   R 
Sbjct: 291 NIGDTFMALSNGIFKSCLHRAVVNRESARK-SMAFFLCPKKD-KVVKPPSDILEKMKTRK 350

Query: 303 YCPFSTEEYRVFREKNYEKIGATLKDFAGIV 324
           Y  F+   +  F +K+Y     TL  F+  V
Sbjct: 351 YPDFTWSMFLEFTQKHYRADVNTLDSFSNWV 371

[Close]
BLAST of Spg015096 vs. ExPASy TrEMBL
Match: A0A6J1GN55 (gibberellin 20-oxidase-like protein OS=Cucurbita moschata OX=3662 GN=LOC111455931 PE=3 SV=1)

HSP 1 Score: 451.1 bits (1159), Expect = 4.0e-123
Identity = 218/308 (70.78%), Postives = 259/308 (84.09%), Query Frame = 0 

Query: 15  VPVIDIHSESLSESSVEALREACREWGFFYIRNHGVSKEFYRKLAAGANELFSFPEETKR 74
           VPVID+  +SL+ES+  +L   C E GFF+IRNHGVSKEFY KL +GA+ELFS PEETK+
Sbjct: 6   VPVIDLSCQSLTESTASSLLRTCNELGFFFIRNHGVSKEFYEKLISGADELFSSPEETKQ 65

Query: 75  KMKLGIESYTPRFVVSPFVESFKVMGPHFSASAAAQGFTESLFGHHAAEFSTLLDEYGSK 134
           ++K+G+ SYTPRFVVSPF ESF+V GP+FSAS AAQGFT+SLFGHHA EFS LLDEYGSK
Sbjct: 66  QLKMGVSSYTPRFVVSPFFESFRVSGPNFSASTAAQGFTQSLFGHHATEFSNLLDEYGSK 125

Query: 135 MKELSKRIIQLLLKILGDNFVNKFYDTEFSNCNGYLRINGCSPQDLNDEEEVVVFEKHTD 194
           MKE+SKR+++LLL ILGDNF NKFYD EFSNCNGYLRING +P D+N EEEV VF+KHTD
Sbjct: 126 MKEVSKRVVKLLLNILGDNFGNKFYDPEFSNCNGYLRINGFTPLDMNAEEEVEVFKKHTD 185

Query: 195 ISCVTILFQDEVGGLQMRSKRGEWIDVRPSEDADDLVVNIGDFMQAWSNERLRSAEHRVV 254
           +S VTI+F +++ GLQMRSK G+WIDVR SED D LVVNIGD+M+AWSN RLRSA HRVV
Sbjct: 186 LSYVTIVFPNDMRGLQMRSKGGDWIDVRSSED-DILVVNIGDYMEAWSNGRLRSAVHRVV 245

Query: 255 LRKNNVKRFSVAFFLIYGDDHKEIYAPSEVVGEGNARLYCPFSTEEYRVFREKNYEKIGA 314
           LRKN  KRFS+AF     D+ +EIYAP+EVVGEG +RLY  FS ++Y+ FRE NYEK+GA
Sbjct: 246 LRKNITKRFSIAFLSTPEDESREIYAPNEVVGEGKSRLYRSFSIQQYKHFRENNYEKVGA 305

Query: 315 TLKDFAGI 323
           T+KDF GI
Sbjct: 306 TIKDFVGI 312

[Close]
BLAST of Spg015096 vs. ExPASy TrEMBL
Match: A0A6J1JSZ7 (gibberellin 20-oxidase-like protein OS=Cucurbita maxima OX=3661 GN=LOC111488065 PE=3 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 3.4e-122
Identity = 215/308 (69.81%), Postives = 260/308 (84.42%), Query Frame = 0 

Query: 15  VPVIDIHSESLSESSVEALREACREWGFFYIRNHGVSKEFYRKLAAGANELFSFPEETKR 74
           VPVID+  +SL+ES+  +L  AC + GFF+IRNHGVSKEFY KL +GA+ELFS PEETK+
Sbjct: 6   VPVIDLGCQSLTESTSSSLLRACNKLGFFFIRNHGVSKEFYEKLISGADELFSSPEETKQ 65

Query: 75  KMKLGIESYTPRFVVSPFVESFKVMGPHFSASAAAQGFTESLFGHHAAEFSTLLDEYGSK 134
           ++K+G+ SYTPRF+VSPF ESF+V GP+FSASAAAQGFT+SLFGHHA EFS LLDEYG K
Sbjct: 66  QLKMGVSSYTPRFLVSPFFESFRVSGPNFSASAAAQGFTQSLFGHHATEFSNLLDEYGRK 125

Query: 135 MKELSKRIIQLLLKILGDNFVNKFYDTEFSNCNGYLRINGCSPQDLNDEEEVVVFEKHTD 194
           MKE+SKR+++LLL +LGDN  NKFYD EFSNCNGYLRING +P D+N EEE+ VF+KHTD
Sbjct: 126 MKEVSKRVVKLLLNVLGDNIENKFYDPEFSNCNGYLRINGFTPLDMNAEEEIEVFKKHTD 185

Query: 195 ISCVTILFQDEVGGLQMRSKRGEWIDVRPSEDADDLVVNIGDFMQAWSNERLRSAEHRVV 254
           +S VTI+FQ+++ GLQMRSK G+WIDVR SED   LVVNIGD+M+AWSN RLRSA HRVV
Sbjct: 186 LSYVTIVFQNDIRGLQMRSKGGDWIDVRLSEDG-ILVVNIGDYMEAWSNGRLRSAVHRVV 245

Query: 255 LRKNNVKRFSVAFFLIYGDDHKEIYAPSEVVGEGNARLYCPFSTEEYRVFREKNYEKIGA 314
           LRKN +KRFS+AF     D+ +EIYAPSEVVGEG +RLY  FS ++Y+ FRE NYEK+GA
Sbjct: 246 LRKNIMKRFSIAFLSTPEDESREIYAPSEVVGEGKSRLYRSFSVQQYKHFRENNYEKVGA 305

Query: 315 TLKDFAGI 323
           T+KDF GI
Sbjct: 306 TIKDFVGI 312

[Close]
BLAST of Spg015096 vs. ExPASy TrEMBL
Match: A0A6J1JW96 (gibberellin 20-oxidase-like protein isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111488064 PE=3 SV=1)

HSP 1 Score: 440.7 bits (1132), Expect = 5.5e-120
Identity = 214/308 (69.48%), Postives = 261/308 (84.74%), Query Frame = 0 

Query: 13  VRVPVIDIHSESLSESSVEALREACREWGFFYIRNHGVSKEFYRKLAAGANELFSFPEET 72
           V VPVIDI S+SL+ES+  +L  AC E+GFF+IRNHG+SKE Y+KL +GA++LFS PEET
Sbjct: 4   VEVPVIDISSQSLTESAASSLLRACNEFGFFFIRNHGLSKELYQKLISGADQLFSSPEET 63

Query: 73  KRKMKLGIESYTPRFVVSPFVESFKVMGPHFSASAAAQGFTESLFGHHAAEFSTLLDEYG 132
           K+K+ +G  SY PRFV+SPF ESFKV GP+FSASA AQGFT+SLFGH A EFS LLDEYG
Sbjct: 64  KQKLTIGASSYIPRFVLSPFFESFKVSGPNFSASANAQGFTQSLFGHQATEFSNLLDEYG 123

Query: 133 SKMKELSKRIIQLLLKILGDNFVNKFYDTEFSNCNGYLRINGCSPQDLN-DEEEVVVFEK 192
           SKMKE+SKR+++LLL ILGDN  NKFYD+EFSNCN Y+RING +P+ +N +EEE+ VF+K
Sbjct: 124 SKMKEVSKRVVKLLLNILGDNIENKFYDSEFSNCNAYIRINGFTPRGMNEEEEELEVFKK 183

Query: 193 HTDISCVTILFQDEVGGLQMRSKRGEWIDVRPSEDADDLVVNIGDFMQAWSNERLRSAEH 252
           HTD+S VTI+FQ+++ GLQMR K G+W+DVRPSED ++LVVNIGDFM+AWSN RLRSA H
Sbjct: 184 HTDLSYVTIVFQNDIRGLQMRLKGGDWMDVRPSED-NNLVVNIGDFMEAWSNGRLRSAVH 243

Query: 253 RVVLRKNNVKRFSVAFFLIYGDDHKEIYAPSEVVGEGNARLYCPFSTEEYRVFREKNYEK 312
           RVVLRKNN+KRFS+AFFL   DD KEIYAPSE+VGEG +RLY PFST++YR+F+EKN   
Sbjct: 244 RVVLRKNNMKRFSMAFFLTPKDDDKEIYAPSEIVGEGKSRLYRPFSTQQYRLFKEKNDVN 303

Query: 313 IGATLKDF 320
            G  LKDF
Sbjct: 304 AGYQLKDF 310

[Close]
BLAST of Spg015096 vs. ExPASy TrEMBL
Match: A0A6J1GMS4 (gibberellin 20-oxidase-like protein isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111455930 PE=3 SV=1)

HSP 1 Score: 432.2 bits (1110), Expect = 1.9e-117
Identity = 212/313 (67.73%), Postives = 260/313 (83.07%), Query Frame = 0 

Query: 15  VPVIDIHSESLSESSVEALREACREWGFFYIRNHGVSKEFYRKLAAGANELFSFPEETKR 74
           +PVIDI  +SL+ES+  +L  AC E+GFF+IRNHG+SKE Y+KL +GA++LFS PEETK+
Sbjct: 6   IPVIDISCQSLTESAASSLLRACNEFGFFFIRNHGLSKELYQKLISGADQLFSSPEETKQ 65

Query: 75  KMKLGIESYTPRFVVSPFVESFKVMGPHFSASAAAQGFTESLFGHHAAEFSTLLDEYGSK 134
           K+ +G  SYTPRFVVSPF ESFKV GP+FSASAAAQGFT+SLFGH A EFS LLDEYGSK
Sbjct: 66  KLTMGASSYTPRFVVSPFFESFKVSGPNFSASAAAQGFTQSLFGHQATEFSNLLDEYGSK 125

Query: 135 MKELSKRIIQLLLKILGDNFVNKFYDTEFSNCNGYLRINGCSPQDLN----DEEEVVVFE 194
           MKE+SKR+++LLL ILGDN  +KFYD+EFSNCN YLRING +P+ +N    +EEE+ VF+
Sbjct: 126 MKEVSKRVVKLLLNILGDNIEDKFYDSEFSNCNAYLRINGFTPRSMNEEEEEEEELEVFK 185

Query: 195 KHTDISCVTILFQDEVGGLQMRSKRGEWIDV--RPSEDADDLVVNIGDFMQAWSNERLRS 254
           KHTD+S VTI+FQ+++ GLQMR K G+W+DV  RPSED D+L+VNIGDFM+AWSN RLRS
Sbjct: 186 KHTDLSYVTIVFQNDIRGLQMRLKGGDWMDVRPRPSED-DNLMVNIGDFMEAWSNGRLRS 245

Query: 255 AEHRVVLRKNNVKRFSVAFFLIYGDDHKEIYAPSEVVGEGNARLYCPFSTEEYRVFREKN 314
           A HRVVLRKNN+KRFS+AFFL   DD KEIYAPSE+VGEG +RLY PFST++YR+F+EK+
Sbjct: 246 AVHRVVLRKNNMKRFSMAFFLTTEDDDKEIYAPSEIVGEGKSRLYRPFSTQQYRLFKEKS 305

Query: 315 YEKIGATLKDFAG 322
                  LKD  G
Sbjct: 306 GVNARYALKDIIG 317

[Close]
BLAST of Spg015096 vs. ExPASy TrEMBL
Match: A0A6J1GMT1 (gibberellin 20-oxidase-like protein OS=Cucurbita moschata OX=3662 GN=LOC111455933 PE=3 SV=1)

HSP 1 Score: 431.4 bits (1108), Expect = 3.3e-117
Identity = 207/308 (67.21%), Postives = 253/308 (82.14%), Query Frame = 0 

Query: 15  VPVIDIHSESLSESSVEALREACREWGFFYIRNHGVSKEFYRKLAAGANELFSFPEETKR 74
           VPVIDI S+SL+ES+  +L  AC E GFF+I+NHG+SKEFY+K  +G + LFS PEETK+
Sbjct: 6   VPVIDISSQSLTESTASSLFRACTELGFFFIKNHGISKEFYQKFISGIDLLFSSPEETKQ 65

Query: 75  KMKLGIESYTPRFVVSPFVESFKVMGPHFSASAAAQGFTESLFGHHAAEFSTLLDEYGSK 134
           ++K G+ SYTPRFV+SPF+E+FK  GP+FSAS+ AQG T+S FGH A EFS LLDEYGSK
Sbjct: 66  RLKHGVSSYTPRFVISPFLETFKFSGPNFSASSTAQGVTQSQFGHQATEFSNLLDEYGSK 125

Query: 135 MKELSKRIIQLLLKILGDNFVNKFYDTEFSNCNGYLRINGCSPQDLNDEEEVVVFEKHTD 194
           M E+SKRI++LLL ILGDN  NKFYD EFSNCNGYLRING +P  +ND+EEV VF+KHTD
Sbjct: 126 MAEVSKRILKLLLNILGDNIENKFYDPEFSNCNGYLRINGYTPLGMNDDEEVEVFKKHTD 185

Query: 195 ISCVTILFQDEVGGLQMRSKRGEWIDVRPSEDADDLVVNIGDFMQAWSNERLRSAEHRVV 254
           +S VTI+F +++ GLQMRSK G+WID  PSED D LVVNIGD ++AWSN RLRSA HRVV
Sbjct: 186 VSYVTIVFPNDIRGLQMRSKGGDWIDAMPSED-DTLVVNIGDLLEAWSNGRLRSAIHRVV 245

Query: 255 LRKNNVKRFSVAFFLIYGDDHKEIYAPSEVVGEGNARLYCPFSTEEYRVFREKNYEKIGA 314
           LRKNN+KRFS+ +     D+ +EI+APSEVVGEG +RLY PFST++Y+ FR KNYEKIG 
Sbjct: 246 LRKNNMKRFSIVYTSTPEDESREIHAPSEVVGEGKSRLYRPFSTQQYKDFRNKNYEKIGE 305

Query: 315 TLKDFAGI 323
           T+KDF GI
Sbjct: 306 TIKDFVGI 312

[Close]
BLAST of Spg015096 vs. TAIR 10
Match: AT4G23340.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 307.8 bits (787), Expect = 1.1e-83
Identity = 166/318 (52.20%), Postives = 219/318 (68.87%), Query Frame = 0 

Query: 13  VRVPVIDIHSESLSESSVEALREACREWGFFYIRNHGVSKEFYRKLAAGANELFSFPEET 72
           +++PV+D+ ++ +  S + +L EAC+EWGFFY+ NHG+SKE + K+ + + ++F  P E+
Sbjct: 8   LQLPVLDL-TQPIESSILSSLSEACKEWGFFYVTNHGISKEMFSKICSLSRDVFKAPLES 67

Query: 73  KRKMKLGIESYTPRFVVSPFVESFKVMGPHFSASAAAQGFTESLF-GHHAAEFSTLLDEY 132
             K+KLG  SYTPR++ SP+ ES  V GP FS SA A    + LF  HH  E    + EY
Sbjct: 68  --KLKLGPISYTPRYIASPYFESLVVSGPDFSDSAKAS--ADVLFQDHHKPELRETMQEY 127

Query: 133 GSKMKELSKRIIQLLLKI-LGDNFVNKFYDTEFSNCNGYLR-INGCSPQDLNDEEEVVV- 192
           G+KM ELSKR+I++LL + LGD    + Y T+FSNC+GYLR +N   P D+  +EE+V  
Sbjct: 128 GAKMAELSKRLIKILLMMTLGDETGKRLYQTDFSNCHGYLRLVNYTPPHDVEKQEELVEG 187

Query: 193 FEKHTDISCVTILFQDEVGGLQMRSKRGEWIDVRPSEDADDLVVNIGDFMQAWSNERLRS 252
              HTD+SC+TI++QD VGGLQMRSK G+WID+ P  D   LVVNIGD MQAWSN RLRS
Sbjct: 188 LGMHTDMSCITIVYQDSVGGLQMRSKEGKWIDINPCNDF--LVVNIGDLMQAWSNGRLRS 247

Query: 253 AEHRVVLRKNNVKRFSVAFFLIYGDDHKEIYAPSEVVGEGNARLYCPFSTEEYRVFREKN 312
           +EHRVVLRK  V R S+AFFL + +D K I AP E+VGEG  R Y  F   EY  FR+ N
Sbjct: 248 SEHRVVLRK-LVNRVSLAFFLCF-EDEKVILAPQEIVGEGKQRSYKSFKCSEYLKFRQSN 307

Query: 313 ----YEKIGATLKDFAGI 323
               +EKIG T+KDFAG+
Sbjct: 308 EEGKFEKIGFTVKDFAGL 316

[Close]
BLAST of Spg015096 vs. TAIR 10
Match: AT5G51310.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 265.8 bits (678), Expect = 4.6e-71
Identity = 149/329 (45.29%), Postives = 218/329 (66.26%), Query Frame = 0 

Query: 13  VRVPVIDIHSESLSESSVEALREACREWGFFYIRNHGVSKEFYRKLAAGANELFSFPEET 72
           + +PV DI S+ LSESS+ +L++AC+EWGFFY+ NHGVS++ Y+KL   +  +F   +E 
Sbjct: 3   LELPVFDI-SKPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKLRRFSTGVFELEDE- 62

Query: 73  KRKMKLGIESYTPRFVVSPFVESFKVMGPHFSASAAAQGFTESLFGHHAA--EFSTLLDE 132
             KMK+G  +YTPRF+ SPF ES +V GP F ASA +   +   F   A   EFS L+ E
Sbjct: 63  -EKMKMGASNYTPRFIASPFFESLRVSGPDFYASAKS---SVDAFSDQATDEEFSGLMKE 122

Query: 133 YGSKMKELSKRIIQLLLKILGDNFVNKFYDTEFSNCNGYLRINGCS-PQDLNDE----EE 192
           YG KM +L ++I++ +L   GD+  +K+Y++EF NC+GY RIN  + P D  D+    +E
Sbjct: 123 YGEKMTKLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDDHHNGDE 182

Query: 193 VVVFE---KHTDISCVTILFQDEVGGLQMRSKRG-EWIDVRPSEDADDLVVNIGDFMQAW 252
             + E    HTD+SC+TI+ QD++GGLQ+R++ G   +D+ P ++A  LVVN+GD + AW
Sbjct: 183 QDLIEGLGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINPKDEA--LVVNVGDLLHAW 242

Query: 253 SNERLRSAEHRVVLRKNNV--KRFSVAFFLIYGDDHKEIYAPSEVVG--EGNARLYCPFS 312
           +N RLRS++HRV+L++      RFS+AFF  + DD K ++AP EVVG  EG  R++  F 
Sbjct: 243 TNGRLRSSQHRVILKRRGFVGNRFSLAFFWCF-DDGKVVFAPDEVVGGCEG-MRVFRSFK 302

Query: 313 TEEYRVFREKN----YEKIGATLKDFAGI 323
             +Y  FRE N    +EK+G T++DFA I
Sbjct: 303 CGDYLRFRESNEKGKFEKVGDTVEDFARI 321

[Close]
BLAST of Spg015096 vs. TAIR 10
Match: AT4G23340.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 217.6 bits (553), Expect = 1.4e-56
Identity = 116/202 (57.43%), Postives = 144/202 (71.29%), Query Frame = 0 

Query: 128 LDEYGSKMKELSKRIIQLLLKI-LGDNFVNKFYDTEFSNCNGYLR-INGCSPQDLNDEEE 187
           + EYG+KM ELSKR+I++LL + LGD    + Y T+FSNC+GYLR +N   P D+  +EE
Sbjct: 1   MQEYGAKMAELSKRLIKILLMMTLGDETGKRLYQTDFSNCHGYLRLVNYTPPHDVEKQEE 60

Query: 188 VVV-FEKHTDISCVTILFQDEVGGLQMRSKRGEWIDVRPSEDADDLVVNIGDFMQAWSNE 247
           +V     HTD+SC+TI++QD VGGLQMRSK G+WID+ P  D   LVVNIGD MQAWSN 
Sbjct: 61  LVEGLGMHTDMSCITIVYQDSVGGLQMRSKEGKWIDINPCNDF--LVVNIGDLMQAWSNG 120

Query: 248 RLRSAEHRVVLRKNNVKRFSVAFFLIYGDDHKEIYAPSEVVGEGNARLYCPFSTEEYRVF 307
           RLRS+EHRVVLRK  V R S+AFFL + +D K I AP E+VGEG  R Y  F   EY  F
Sbjct: 121 RLRSSEHRVVLRK-LVNRVSLAFFLCF-EDEKVILAPQEIVGEGKQRSYKSFKCSEYLKF 180

Query: 308 REKN----YEKIGATLKDFAGI 323
           R+ N    +EKIG T+KDFAG+
Sbjct: 181 RQSNEEGKFEKIGFTVKDFAGL 198

[Close]
BLAST of Spg015096 vs. TAIR 10
Match: AT4G21200.1 (gibberellin 2-oxidase 8 )

HSP 1 Score: 123.6 bits (309), Expect = 2.8e-28
Identity = 96/309 (31.07%), Postives = 147/309 (47.57%), Query Frame = 0 

Query: 11  EEVRVPVIDIH-----SESLSESSVEALREACREWGFFYIRNHGVSKEFYRKLAAGANEL 70
           EEV +PVID+      +E   E   EA+  A REWGFF + NHG+S +   K+      +
Sbjct: 38  EEVELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRV 97

Query: 71  FSFPEETKRKM-KLGIESYTPRFVVSPFVESFKVMGPHFSASAAAQGFTESLFGHHAAEF 130
           F  P + K K  K    SY      +P   S + +     + A     T+          
Sbjct: 98  FREPFDKKSKSEKFSAGSYR---WGTPSATSIRQLS---WSEAFHVPMTDISDNKDFTTL 157

Query: 131 STLLDEYGSKMKELSKRIIQLLLKILGDNFVNKFYDTEFSNCNGYLRINGCSPQDLNDEE 190
           S+ ++++ S+ + L+  + ++L +  G N  + F+         YLR+N   P       
Sbjct: 158 SSTMEKFASESEALAYMLAEVLAEKSGQN--SSFFKENCVRNTCYLRMNRYPP--CPKPS 217

Query: 191 EVVVFEKHTDISCVTILFQDEVGGLQMRSKRGEWIDVRPSEDADDLVVNIGDFMQAWSNE 250
           EV     HTD   +TIL+QD+VGGLQ+  K   WI V+P+  A  L++NIGD  QAWSN 
Sbjct: 218 EVYGLMPHTDSDFLTILYQDQVGGLQL-IKDNRWIAVKPNPKA--LIINIGDLFQAWSNG 277

Query: 251 RLRSAEHRVVLRKNNVKRFSVAFFLIYGDDHKEIYAPSEVVGEGNARLYCPFSTEEYRVF 310
             +S EHR V+    V+RFS A+F+    D       + +    +   Y  FS  E+R  
Sbjct: 278 MYKSVEHR-VMTNPKVERFSTAYFMCPSYD-------AVIECSSDRPAYRNFSFREFRQQ 325

Query: 311 REKNYEKIG 314
            +++ +K G
Sbjct: 338 VQEDVKKFG 325

[Close]
BLAST of Spg015096 vs. TAIR 10
Match: AT1G55290.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 122.5 bits (306), Expect = 6.3e-28
Identity = 104/323 (32.20%), Postives = 150/323 (46.44%), Query Frame = 0 

Query: 8   RSSEEVRVPVIDIHSESLSESSV-EALREACREWGFFYIRNHGVSKEFYRKLAAGANELF 67
           +   +  +PVIDI   +L E SV +A+ +A  EWGFF + NHGVS E    +    +  F
Sbjct: 55  KEDSDESIPVIDI--SNLDEKSVSKAVCDAAEEWGFFQVINHGVSMEVLENMKTATHRFF 114

Query: 68  SFPEETKRKMKLGIESYTPRFVVSPFVESFKVMGPHFSASAAAQGFTESLFGHHAAEFST 127
             P E KRK       ++    +S  V       PH   +   + +    F   A     
Sbjct: 115 GLPVEEKRK-------FSREKSLSTNVRFGTSFSPHAEKALEWKDYLSLFFVSEAEASQL 174

Query: 128 LLDEYGSKMKEL---SKRIIQLLLKILGDNF-VNKFYDTEFSNCNGYLRINGCSPQDLND 187
             D   S+  E    +K +++ LL+ LG+N  V +   T+ S   G  RIN        +
Sbjct: 175 WPDSCRSETLEYMNETKPLVKKLLRFLGENLNVKELDKTKESFFMGSTRINLNYYPICPN 234

Query: 188 EEEVVVFEKHTDISCVTILFQDEVGGLQMRS-KRGEWIDVRPSEDADDLVVNIGDFMQAW 247
            E  V   +H+D+S +TIL QDE+GGL +RS   G W+ V P   +  LV+NIGD MQ  
Sbjct: 235 PELTVGVGRHSDVSSLTILLQDEIGGLHVRSLTTGRWVHVPPI--SGSLVINIGDAMQIM 294

Query: 248 SNERLRSAEHRVVLRKNNVKRFSVAFFLIYGDDHKEIYAP-SEVVGEGNARLYCPFSTEE 307
           SN R +S EHR VL   +  R SV  F+      + +  P  EV+  G   +Y      +
Sbjct: 295 SNGRYKSVEHR-VLANGSYNRISVPIFV--SPKPESVIGPLLEVIENGEKPVYKDILYTD 354

Query: 308 Y-RVFREKNYEKIGATLKDFAGI 323
           Y + F  K ++  G    DFA I
Sbjct: 355 YVKHFFRKAHD--GKKTIDFANI 361

The following BLAST results are available for this feature:
BLAST of Spg015096 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038899419.11.2e-12471.38gibberellin 20-oxidase-like protein [Benincasa hispida][more]
XP_022953358.18.3e-12370.78gibberellin 20-oxidase-like protein [Cucurbita moschata][more]
XP_022991455.17.1e-12269.81gibberellin 20-oxidase-like protein [Cucurbita maxima][more]
KAG6576056.16.0e-12169.81Gibberellin 20-oxidase-like protein, partial [Cucurbita argyrosperma subsp. soro... [more]
XP_022991453.11.1e-11969.48gibberellin 20-oxidase-like protein isoform X1 [Cucurbita maxima][more]
BLAST of Spg015096 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
F4KBY06.5e-7045.29Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310... [more]
O495614.0e-2731.07Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 ... [more]
Q9C8998.9e-2732.20Feruloyl CoA ortho-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=F6'H2 PE=1 S... [more]
Q9C6I44.4e-2630.09Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 ... [more]
Q391111.7e-2530.51Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GA20OX2 PE=2 SV=1[more]
BLAST of Spg015096 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1GN554.0e-12370.78gibberellin 20-oxidase-like protein OS=Cucurbita moschata OX=3662 GN=LOC11145593... [more]
A0A6J1JSZ73.4e-12269.81gibberellin 20-oxidase-like protein OS=Cucurbita maxima OX=3661 GN=LOC111488065 ... [more]
A0A6J1JW965.5e-12069.48gibberellin 20-oxidase-like protein isoform X1 OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1GMS41.9e-11767.73gibberellin 20-oxidase-like protein isoform X1 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1GMT13.3e-11767.21gibberellin 20-oxidase-like protein OS=Cucurbita moschata OX=3662 GN=LOC11145593... [more]
BLAST of Spg015096 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G23340.11.1e-8352.202-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT5G51310.14.6e-7145.292-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G23340.21.4e-5657.432-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G21200.12.8e-2831.07gibberellin 2-oxidase 8 [more]
AT1G55290.16.3e-2832.202-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00682IPNSYNTHASEcoord: 230..256
score: 26.94
coord: 33..50
score: 57.07
NoneNo IPR availablePANTHERPTHR47990:SF9GIBBERELLIN 20-OXIDASE-LIKE PROTEINcoord: 9..312
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 9..312
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 10..309
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 15..77
e-value: 5.3E-17
score: 62.6
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 181..269
e-value: 1.2E-18
score: 67.5
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 1..311
e-value: 8.8E-68
score: 231.0
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 171..271
score: 10.582811

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg015096.1Spg015096.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0048767 root hair elongation
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding