Spg015094 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg015094
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Description7-dehydrocholesterol reductase
Locationscaffold3: 49983497 .. 49989322 (-)
RNA-Seq ExpressionSpg015094
SyntenySpg015094
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTATTACTTCTCCTTACTTTTTGTCCCCTAAATTAGTAGTCCGAGAAATTTAAAGCTCCTAAAAGAAAGAAGATTAAAAAAAAAAAAAAGTAAGTCCGCCATTTAAGTCCCACCTCCAATTGGGTGAAGTCATTTCCAGGTTGACTAGACTCCGGCTGAAACTCAGACACCGCAAAAATGGCGGAAGGATCCAACACCGTCCATTCACCACTCCTCACTTATGTTTCCATGTTATCTCTTCTCACTCTCTGCCCTCCCTTTGTTATCTTCCTGTAATTCTCTATCTCTCTGCTTCTTAATCCGAGTGGATCTCATTCATTTTCTCTATTTTCAAAGATTCATCACAATTCTTGCTTCCACTGTGCTTTCCCTTGTAGTTGATCTGATCATCATTCATCACAACAAACGGATTAACACGCGCTTCTTGTGTTTGTGTGCTCTCAATTGCTCCATCCATCATACTAATGGCTTCTTTGCTTTGTTTGAATATCTCGACGTTGTTTTTTTCTCACATGCAGATGGTACACAATGGTCCATACTGATGGGTCTGTTTCCCAGTCTTGGAATTACTTATACCAGAATGGATTGAGCGGGTTTATAAATATATGGCCACCGCCTTCTGCTACTGCTTGCAAAATAATTGGATGTTACGCTACCTTTGAGGCTGCCCTTCAGCTTCTTTTACCTGGAAAGAGGGTTCAGGGTCCCGTCTCTCCAACTGGAAATCAACCTGTCTACAAGGTATTTGGACATTTGACTCTCGATCACTCTCTGTCAGTTCTCTGCATTTCATAGTCCACTGAACTTACTAATGCCTTGAAATTCATGATGTTGTTAATGTTATGTTAGTAATTTATTACTCCTGTTTCTGTGTTGCAGGCAAATGGCGTGGCTGCGTACATAGTGACTCTGGTTACTTATATTGCTCTTTGGTGGTATGGTATTCAATCCACCTTCTACCTTGATCTCGTTAAAAATATTTTAGAGATTTGATTTTTGAATTGGAAACTGGATCAGTTTCCTCATTTCGCAGTTCCTAGTTGTCACTGGTGCTCATAGATTAGAGTTATAGTCATGACTCACGAGTACTCTTGTTGTACAATTGTTCTTCACCTCTCCTCTATGTCAGCATTTTCCTAGTAAGCGATGTTCTTTCTTCTTTGGCAATTGAAATGTTTGAGACATGTTTTATATTAAACATCCATGATGCGGGAGACGTTTTGGGGGGGGTGGAATAGTCTGTATCATCAGCTTAGACAAATCACATTTTTGTAATTCTTTCTGCTGTACTAATTTTTCGTTGATCTGTTTTTTCCATCTATATCTCTCATTCTTAGTCTATTATTGGCATACTGTCGCTCCAACAGACTCCGTAAAATCTTCATGGTCTTTTGTGCTAGAATTATATGCCTGTACATGATTTGGATAGGAAATATCCTTTCGGCTCTGTGGATTCATTATGATATTTTTCTTGTTTCATAGTTTAACTGGAACTTCTATTCTTCAGGTTTGGGATATTCAATCCAACAATCGCTTATGATCATTTGGGAGAAATATTTTCCGCTCTTATTCTTGGAAGCTTCATCTTTTGTATTATTCTATACATAAAAGTGAGGATTTAGACTGTTGTGTCGATACCAGTTGAAGTTTAGTGTATATATGCTCTTTGTATATGTAACACTGATGTTTGCATGTATAAGGGTCGTGTGGTGCCATCTTCCTCTGATTCTGGTTCCTCTGGTAACATAATAATTGATTTCTACTGGGTAAGTTTTCACATCCCTTTGATATTTATAATTTGACATAGCAATTTTCATTTTTCTTTAGTAAAAAGAAAAAACCTGCTATTTTATAAATGAATGATATTACAGAGAAGGCGACACAAAAAACAGGCCCAACCAACATGGGGACAACAAAAGAGAAAAGAGAAAATTTTAAAAATTACAAAATGGAACTGTTAGGTATCTAATCTGAAAAACCTGTTAAAAAACACACAGCAAGATAGCAAGATCCAACACTGTTCTATTAGGAGTTCTCCAGTGAACAGTGGGATTTATGAGCCAATTGTATTGATAGAACAGCCAAACAGAATACAATATGCAAACCATCCAGCCAATTCAAGAGGGCTGAAATCTCTCCTCAGACTTCTACAGTCCACAACAAAAAACATACCCTCCCCTCACTCAGCCTCTCTTTACATATGCCCTTCCTCAACAGACGCACGATCCCAAACGGTCCCCACATTCCCTACTTTCGTTCCATTTCCTCTTCTACTCCTCCTATGGTATGTTTGTGTTATGGGAGGTCTAACAGGAACCTCAGCTGTTGGCAATAAGAAAAATAGGAAGCCTAAAAAAATTTGAAGAGCAAACATAATTAAAGGCATTAAAATGTACCAGCCATCAAACCTCCTCTCATGAGCTATCAACAGCTTAAAAGATTCTCCATTTCCTTCCCAACCAAATATTCCATAAAACAATGAATAACCAGACAGCCTTTATCTAAAAATGGAGGACTCAAAAGAACTTTCTCCGTCACTGTGAGTAAACTAGAACTTGGAACCGTGAAAATACCAAAAGTCTACCAAAAATAACACCGCCCTTTGCGACAATGTGATTCATGGTATAGATGATTATTGAACTAAGTTACTTGTGAATGAACTAATGAACTCTACAGATAAAATTATTAGGGTAATTTGGCACAAATCAGATAATAGTCTTCTAAGAAATTCAAGTAGTTTGTATATGCACTTATTGTCTTGAAAAGTTCTATGGTTTTGTTATTTATAATGCTACTATATATCAAATATATAAACATAGTTATTATCTTTTGCTTTATCAAGTGTTCTCTGCTTTTCACTTCACTAGTCAGCCCGTCTTTAATTCCTGGTCTGCTTAGTATGTATGGTATGATAACAAAATGTTGCAAGCCTGACTGGTTTGGCATGAACCATGTTTCTTTTTGTTATTGCTTCAATTTTAATTTAATTCTGGATGATTTCTTGGTGCGGCCAATATCTTTTTATGATTTATTTTGATTCCAGCTGCTAAGTAATCTCCATCTAGCATTTCATGCTAAATGCTCTTAGCCTTGTGAAGTTATATCTTTATATGTAGCTTATAGTCTTTCAATTTGGAAATTAGGAAAAGTAGATACTCTATACTTAAGGATTCATCTTCATATAACTTGTTTATATTTTGCTCCCACGTTTTTTTCTTCAAGTTTTTATTTGCTTAATTTTCTCTTTAGTTCCATTTTAAAGCATCTGTAAAAAGAAAATTGAATTAGAACTAGAGTATAGTATTTCGTTTGATCCCATGTTTGCATTGGCTAATCTAGGTCTTGTTCTTTAACACCCTTTTTATGTACTTTCTTGCAGGGCATGGAGTTGTACCCACGTATTGGCAAACATTTTGATATTAAAGTTTTCACAAACTGCAGATTTGGGATGATGTCCTGGGCAGTTCTCGCTGTGACCTATTGCATAAAGCAGGTAGGTATTCTAAAAGATCAACAAGTTTGAAAAATAATCCTACATTTGTGGAAGTTTATCCTTCATGTGAGGCTTACCAGATATTATTTAGAATAATGGGATTCAAGCCTCATTTAATTGGTTGGTACCTTTGCTTTATCTTTTACTGGTATTTTCTCCCGTTAAATTTTTTAGGCTACAGAAGTTAACATTCCTTTAGATGAATTTAAAACTGGTGGTTGTTTTTGTAGTTTCAATTTTTATCTTATTTTTCCTCCTTAGATTCGCTCTTCTTCCTTTTAGTACAAGTAATGCAAACTAGCAGCCATAAATTCCTATCAGAACATTTTCTTTCTAATTAGCCTGAATCATTAAGATCCTTATCCTTAAGTTCGCTTTCACCATTTTATTTCCTGTACTGCATTGAAGAATGGTTTGGCATAGTGAAGCACGTATATGTATATTGTACTGTAGGTTCTATGGTGTTCAAAGTGTTGAGTGATTTAAAGTGTTAAATAATTTTCTTCTCATTTCATCCATCCAGTATGAAAAGAACGGGACAATTGCTGATTCAATGCTTGTCAATACTGCATTGATATTGGTTTATGTTTCAAAGTTTTTTTGGTGGGAAGCAGGGTACTGGAACACAATGGACATAGCGCATGATAGAGGTTAGCTTATATTGTATGATTTTCTTTGTTTAATCTATTATTGTTTTCATACTCTTCTGTCATTAATTAAAATGCTGGAGAACAGCTGACTAGTTATCGATTTTGAAAAATATGATATATAATTCTGCAACTCATATGTGGTTAAACTGTAATGAGATGTGATTTTAATGGTCCATTTACCTTGTTGGAATTAAGAATAAATAAAAATTTGTTATTCTCGTTGTTTTATCAATCTCAATATCCCTGGAAATTGGCAACTTGGATTTTGCAGCTGGTTTTTATATTTGTTGGGGATGCTTAGTATGGCTTCCGTCTGTATATACCTCTCCTGGCATGTATTTGGTCAACCATTCTGTAAACCTTGGAACTCAGGTATCCTTTCTGTCTGGTTAACTTCTAACATTAAGCAATTAATTCGAATTGAAACATGTTGGTTAGTCATATTTTTAGAGATTCGTAGAAACTCATTAGCTTTTGGGTCACTTATCAAAAATAAATAAATAAGCTTTTGGATGGATGTTGGTTGGTTAGCCAATGTGGAAAGTATCCAACTTTTAAGATCACCATCTTGATGTATTTATAAGCATTTAGGTATCAAATATGAAAGCTATGGTTTGAATAACTAGATACTCCTTTGTTATGTTTGCATGGTTGTTTCTACTTGTTGTATAATTCTTTATGGATCATGCTGTTTTCCTAATCATCCCTTTGGATTTCAGTTGGCAGTATATATACTAGTAGCAGGCATTCTTTGCATATATATCAACTATGATTGTGACAGGCAGAGGCAAGAATTTCGCAGAAAAAGTGGGAAGTGCTTGGTTTGGGGAAAAGCTCCATCAAAGGTTTTGACGAGAAGTCTGTGTCTATAAGAATTCATATAGAACTAACTAGTCATATGCTGTGATTAAATCTGATATTAATTTGACAGATAGTAGCTTCGTATACCACGACATCTGGGGAAACCAGAACTAGCCTTCTTTTAACCTCTGGCTGGTGAGTTTTCATTTGTTTCATTACCTACATAGACACAAGTGTAGGTGGGGAATTATTAGGCATTTTGTTGTTCGTGTTGGTGCAGGTGGGGTTTAGCACGGCATTTTCATTATGTACCAGAAATTTTGGCTGCATTTTTCTGGACAGTTCCAGCCCTTTTTAATCATGTAAGCATCATCTATTCTAATCGACACGAACACGTACTTTTCACCCGAAAGAAGAATTATATATTTTTTTGGTAATTGTTGGTTTGGATCTCCTCCTCTGCAGTTTCTACCATATTTCTATGTGATATTTCTTACTATTCTTCTGTTCGACCGAGCTAAAAGGGATGACGATCGATGCCGCTCCAAGTAAGTAAATCAGTTTGGTTCGCAGCTGATATGGGATGGGAGTATTTTGGTTGATGATTTTTCATGTATGCACGCAGGTATGGCAAATATTGGAAACTGTACTGCGAGAAAGTGCCTTACAGAATCATTCCTGGAATATACTAATACTAGTTCCCTTCCTACTCTTGTGTTGTTCACTTCATGTACACTATTACTTATGTTGTTCACTTCATGTAAACTCATGTTGCTCACCAAACTTATAATTAGATTTAAACTGAAGTTATTTTAAGACATTATTATCCTATATTTTTCATCCCTCTATCAGTTATCTCTCTCTGCGAGTGGGTTTTCAC

mRNA sequence

ATGGCGGAAGGATCCAACACCGTCCATTCACCACTCCTCACTTATGTTTCCATGTTATCTCTTCTCACTCTCTGCCCTCCCTTTGTTATCTTCCTATGGTACACAATGGTCCATACTGATGGGTCTGTTTCCCAGTCTTGGAATTACTTATACCAGAATGGATTGAGCGGGTTTATAAATATATGGCCACCGCCTTCTGCTACTGCTTGCAAAATAATTGGATGTTACGCTACCTTTGAGGCTGCCCTTCAGCTTCTTTTACCTGGAAAGAGGGTTCAGGGTCCCGTCTCTCCAACTGGAAATCAACCTGTCTACAAGGCAAATGGCGTGGCTGCGTACATAGTGACTCTGGTTACTTATATTGCTCTTTGGTGGTATGGTATTCAATCCACCTTCTACCTTGATCTCGGTCGTGTGGTGCCATCTTCCTCTGATTCTGGTTCCTCTGGTAACATAATAATTGATTTCTACTGGGGCATGGAGTTGTACCCACGTATTGGCAAACATTTTGATATTAAAGTTTTCACAAACTGCAGATTTGGGATGATGTCCTGGGCAGTTCTCGCTGTGACCTATTGCATAAAGCAGTATGAAAAGAACGGGACAATTGCTGATTCAATGCTTGTCAATACTGCATTGATATTGGTTTATGTTTCAAAGTTTTTTTGGTGGGAAGCAGGGTACTGGAACACAATGGACATAGCGCATGATAGAGCTGGTTTTTATATTTGTTGGGGATGCTTAGTATGGCTTCCGTCTGTATATACCTCTCCTGGCATGTATTTGGTCAACCATTCTGTAAACCTTGGAACTCAGTTGGCAGTATATATACTAGTAGCAGGCATTCTTTGCATATATATCAACTATGATTGTGACAGGCAGAGGCAAGAATTTCGCAGAAAAAGTGGGAAGTGCTTGGTTTGGGGAAAAGCTCCATCAAAGATAGTAGCTTCGTATACCACGACATCTGGGGAAACCAGAACTAGCCTTCTTTTAACCTCTGGCTGGTGGGGTTTAGCACGGCATTTTCATTATGTACCAGAAATTTTGGCTGCATTTTTCTGGACAGTTCCAGCCCTTTTTAATCATTTTCTACCATATTTCTATGTGATATTTCTTACTATTCTTCTGTTCGACCGAGCTAAAAGGGATGACGATCGATGCCGCTCCAAGTATGGCAAATATTGGAAACTGTACTGCGAGAAAGTGCCTTACAGAATCATTCCTGGAATATACTAA

Coding sequence (CDS)

ATGGCGGAAGGATCCAACACCGTCCATTCACCACTCCTCACTTATGTTTCCATGTTATCTCTTCTCACTCTCTGCCCTCCCTTTGTTATCTTCCTATGGTACACAATGGTCCATACTGATGGGTCTGTTTCCCAGTCTTGGAATTACTTATACCAGAATGGATTGAGCGGGTTTATAAATATATGGCCACCGCCTTCTGCTACTGCTTGCAAAATAATTGGATGTTACGCTACCTTTGAGGCTGCCCTTCAGCTTCTTTTACCTGGAAAGAGGGTTCAGGGTCCCGTCTCTCCAACTGGAAATCAACCTGTCTACAAGGCAAATGGCGTGGCTGCGTACATAGTGACTCTGGTTACTTATATTGCTCTTTGGTGGTATGGTATTCAATCCACCTTCTACCTTGATCTCGGTCGTGTGGTGCCATCTTCCTCTGATTCTGGTTCCTCTGGTAACATAATAATTGATTTCTACTGGGGCATGGAGTTGTACCCACGTATTGGCAAACATTTTGATATTAAAGTTTTCACAAACTGCAGATTTGGGATGATGTCCTGGGCAGTTCTCGCTGTGACCTATTGCATAAAGCAGTATGAAAAGAACGGGACAATTGCTGATTCAATGCTTGTCAATACTGCATTGATATTGGTTTATGTTTCAAAGTTTTTTTGGTGGGAAGCAGGGTACTGGAACACAATGGACATAGCGCATGATAGAGCTGGTTTTTATATTTGTTGGGGATGCTTAGTATGGCTTCCGTCTGTATATACCTCTCCTGGCATGTATTTGGTCAACCATTCTGTAAACCTTGGAACTCAGTTGGCAGTATATATACTAGTAGCAGGCATTCTTTGCATATATATCAACTATGATTGTGACAGGCAGAGGCAAGAATTTCGCAGAAAAAGTGGGAAGTGCTTGGTTTGGGGAAAAGCTCCATCAAAGATAGTAGCTTCGTATACCACGACATCTGGGGAAACCAGAACTAGCCTTCTTTTAACCTCTGGCTGGTGGGGTTTAGCACGGCATTTTCATTATGTACCAGAAATTTTGGCTGCATTTTTCTGGACAGTTCCAGCCCTTTTTAATCATTTTCTACCATATTTCTATGTGATATTTCTTACTATTCTTCTGTTCGACCGAGCTAAAAGGGATGACGATCGATGCCGCTCCAAGTATGGCAAATATTGGAAACTGTACTGCGAGAAAGTGCCTTACAGAATCATTCCTGGAATATACTAA

Protein sequence

MAEGSNTVHSPLLTYVSMLSLLTLCPPFVIFLWYTMVHTDGSVSQSWNYLYQNGLSGFINIWPPPSATACKIIGCYATFEAALQLLLPGKRVQGPVSPTGNQPVYKANGVAAYIVTLVTYIALWWYGIQSTFYLDLGRVVPSSSDSGSSGNIIIDFYWGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALILVYVSKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWLPSVYTSPGMYLVNHSVNLGTQLAVYILVAGILCIYINYDCDRQRQEFRRKSGKCLVWGKAPSKIVASYTTTSGETRTSLLLTSGWWGLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWKLYCEKVPYRIIPGIY
Homology
BLAST of Spg015094 vs. NCBI nr
Match: XP_022147941.1 (7-dehydrocholesterol reductase [Momordica charantia])

HSP 1 Score: 796.6 bits (2056), Expect = 1.0e-226
Identity = 376/435 (86.44%), Postives = 392/435 (90.11%), Query Frame = 0

Query: 1   MAEGSNTVHSPLLTYVSMLSLLTLCPPFVIFLWYTMVHTDGSVSQSWNYLYQNGLSGFIN 60
           MAEGS TVHSP+LTYVSMLSLL +CPPFVIFLWYTMVH DGSVSQ+WNYLY NG+ GF+N
Sbjct: 1   MAEGSTTVHSPILTYVSMLSLLIICPPFVIFLWYTMVHADGSVSQTWNYLYHNGVRGFMN 60

Query: 61  IWPPPSATACKIIGCYATFEAALQLLLPGKRVQGPVSPTGNQPVYKANGVAAYIVTLVTY 120
           IWPPPSATACKIIGCYA FEAALQLLLPG RVQGPVSPTGNQPVYKANGVAAYIVTLVTY
Sbjct: 61  IWPPPSATACKIIGCYAAFEAALQLLLPGNRVQGPVSPTGNQPVYKANGVAAYIVTLVTY 120

Query: 121 IALWWYGIQS-----------------------TFYLDLGRVVPSSSDSGSSGNIIIDFY 180
           IALWW+GI +                        F    G V PSSSDSGSSGNIIIDFY
Sbjct: 121 IALWWFGIFNPTIVYDHLGEIFSALIFGSLIFCIFLYIKGHVAPSSSDSGSSGNIIIDFY 180

Query: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALILVY 240
           WGMELYPRIGK+FDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTAL+LVY
Sbjct: 181 WGMELYPRIGKNFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLVY 240

Query: 241 VSKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWLPSVYTSPGMYLVNHSVNLGTQLAVYI 300
           V+KFFWWE+GYWNTMDIAHDRAGFYICWGCLVW+PSVYTSPGMYLVNH VNLGTQLAVYI
Sbjct: 241 VTKFFWWESGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVYI 300

Query: 301 LVAGILCIYINYDCDRQRQEFRRKSGKCLVWGKAPSKIVASYTTTSGETRTSLLLTSGWW 360
           LVAG+LCIYINYDCDRQRQEFRRK+GKCL+W KAPSKIVASYTTTSGET+TSLLLTSGWW
Sbjct: 301 LVAGLLCIYINYDCDRQRQEFRRKNGKCLIWRKAPSKIVASYTTTSGETKTSLLLTSGWW 360

Query: 361 GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK 413
           GLARHFHY PEILAAFFWTVPALFNHFLPYFYVIFLTILL DRAKRDDDRCRSKYGKYWK
Sbjct: 361 GLARHFHYAPEILAAFFWTVPALFNHFLPYFYVIFLTILLLDRAKRDDDRCRSKYGKYWK 420

BLAST of Spg015094 vs. NCBI nr
Match: XP_011659586.1 (7-dehydrocholesterol reductase isoform X1 [Cucumis sativus] >KGN45416.1 hypothetical protein Csa_016668 [Cucumis sativus])

HSP 1 Score: 794.7 bits (2051), Expect = 3.9e-226
Identity = 377/435 (86.67%), Postives = 393/435 (90.34%), Query Frame = 0

Query: 1   MAEGSNTVHSPLLTYVSMLSLLTLCPPFVIFLWYTMVHTDGSVSQSWNYLYQNGLSGFIN 60
           MAEGSNTVHSP LTYVSMLSLLTLCPPFVI LWYTMVH DGSVSQ+WNYLYQNG  GFIN
Sbjct: 1   MAEGSNTVHSPFLTYVSMLSLLTLCPPFVILLWYTMVHADGSVSQTWNYLYQNGFEGFIN 60

Query: 61  IWPPPSATACKIIGCYATFEAALQLLLPGKRVQGPVSPTGNQPVYKANGVAAYIVTLVTY 120
           IWP PSATACKIIGCYA FEAALQLLLPGKRV GPVSPTGNQPVYKANGVAAYIVTL+TY
Sbjct: 61  IWPSPSATACKIIGCYAAFEAALQLLLPGKRVLGPVSPTGNQPVYKANGVAAYIVTLLTY 120

Query: 121 IALWWYGI---------QSTFYLDL--------------GRVVPSSSDSGSSGNIIIDFY 180
           +ALW +GI             Y  L              G V PSSSDSGSSGN+I+DFY
Sbjct: 121 VALWRFGIFDPAIVYDHLGEIYSTLIFGSFIFCILLYIKGHVAPSSSDSGSSGNVILDFY 180

Query: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALILVY 240
           WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGT+ADS+LVNTAL+LVY
Sbjct: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTLADSLLVNTALMLVY 240

Query: 241 VSKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWLPSVYTSPGMYLVNHSVNLGTQLAVYI 300
           V+KFFWWEAGYWNTMDIAHDRAGFYICWGCLVW+PSVYTSPGMYLVNH VNLGTQLAV I
Sbjct: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVSI 300

Query: 301 LVAGILCIYINYDCDRQRQEFRRKSGKCLVWGKAPSKIVASYTTTSGETRTSLLLTSGWW 360
           LVAGILCIYINYDCDRQRQEFRRK+GKCLVWGKAPSKIVA+YTTT+GETRTSLLLTSGWW
Sbjct: 301 LVAGILCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVATYTTTTGETRTSLLLTSGWW 360

Query: 361 GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK 413
           GLARHFHYVPEILAAFFWTVPALF+HFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK
Sbjct: 361 GLARHFHYVPEILAAFFWTVPALFHHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK 420

BLAST of Spg015094 vs. NCBI nr
Match: XP_008462206.1 (PREDICTED: 7-dehydrocholesterol reductase [Cucumis melo] >KAA0059337.1 7-dehydrocholesterol reductase [Cucumis melo var. makuwa] >TYK03988.1 7-dehydrocholesterol reductase [Cucumis melo var. makuwa])

HSP 1 Score: 792.3 bits (2045), Expect = 1.9e-225
Identity = 380/435 (87.36%), Postives = 392/435 (90.11%), Query Frame = 0

Query: 1   MAEGSNTVHSPLLTYVSMLSLLTLCPPFVIFLWYTMVHTDGSVSQSWNYLYQNGLSGFIN 60
           MAEGSNTVHSP LTYVSMLSLLTLCPPFVI LWYTMVH DGSVSQ+WNYLYQNG  GFIN
Sbjct: 1   MAEGSNTVHSPFLTYVSMLSLLTLCPPFVILLWYTMVHADGSVSQTWNYLYQNGFEGFIN 60

Query: 61  IWPPPSATACKIIGCYATFEAALQLLLPGKRVQGPVSPTGNQPVYKANGVAAYIVTLVTY 120
           IWP PSATACKIIGCYA FEAALQLLLPGKRV GPVSPTGNQPVYKANGVAAYIVTLVTY
Sbjct: 61  IWPSPSATACKIIGCYAAFEAALQLLLPGKRVLGPVSPTGNQPVYKANGVAAYIVTLVTY 120

Query: 121 IALWWYGI--QSTFYLDL---------------------GRVVPSSSDSGSSGNIIIDFY 180
           +ALW +GI   +  Y  L                     G V PSSSDSGSSGNII+DFY
Sbjct: 121 VALWRFGIFDPAIVYDHLGEIFSTLIFGSFIFCILLYIKGHVAPSSSDSGSSGNIILDFY 180

Query: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALILVY 240
           WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNG +ADSMLVNTAL+LVY
Sbjct: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGILADSMLVNTALMLVY 240

Query: 241 VSKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWLPSVYTSPGMYLVNHSVNLGTQLAVYI 300
           V+KFFWWEAGYWNTMDIAHDRAGFYICWGCLVW+PSVYTSPGMYLVNH VNLGTQLAV I
Sbjct: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVSI 300

Query: 301 LVAGILCIYINYDCDRQRQEFRRKSGKCLVWGKAPSKIVASYTTTSGETRTSLLLTSGWW 360
           LVAGILCIYINYDCDRQRQEFRRK+GK LVWGKAPSKIVASYTTTSGETRTSLLLTSGWW
Sbjct: 301 LVAGILCIYINYDCDRQRQEFRRKNGKRLVWGKAPSKIVASYTTTSGETRTSLLLTSGWW 360

Query: 361 GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK 413
           GLARHFHYVPEILAAFFWTVPALF+HFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK
Sbjct: 361 GLARHFHYVPEILAAFFWTVPALFHHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK 420

BLAST of Spg015094 vs. NCBI nr
Match: XP_022964233.1 (7-dehydrocholesterol reductase [Cucurbita moschata])

HSP 1 Score: 784.6 bits (2025), Expect = 4.0e-223
Identity = 375/434 (86.41%), Postives = 389/434 (89.63%), Query Frame = 0

Query: 1   MAEGSNTVHSPLLTYVSMLSLLTLCPPFVIFLWYTMVHTDGSVSQSWNYLYQNGLSGFIN 60
           MAEGSNTVHSPL+TY SMLSLLTLCPPFVIFLWYTMVH DGSVSQ+  YLY NGL G I+
Sbjct: 1   MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGLID 60

Query: 61  IWPPPSATACKIIGCYATFEAALQLLLPGKRVQGPVSPTGNQPVYKANGVAAYIVTLVTY 120
           IWPPPSATA K+IG YA FEAALQLLLPGK V GP+SPTGNQPVYKANGVAAY+VTLVTY
Sbjct: 61  IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY 120

Query: 121 IALWWYGI--QSTFYLDLGR---------------------VVPSSSDSGSSGNIIIDFY 180
           IALWW+GI   +  Y  LG                      V PSSSDSGSSGNIIIDFY
Sbjct: 121 IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY 180

Query: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALILVY 240
           WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTAL+L Y
Sbjct: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY 240

Query: 241 VSKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWLPSVYTSPGMYLVNHSVNLGTQLAVYI 300
           V+KFFWWEAGYWNTMDIAHDRAGFYICWGCLVW+PSVYTSPGMYLVNH VNLGTQLAVYI
Sbjct: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVYI 300

Query: 301 LVAGILCIYINYDCDRQRQEFRRKSGKCLVWGKAPSKIVASYTTTSGETRTSLLLTSGWW 360
           LVAG+LCIYINYDCDRQRQEFRRK+GKCLVWGKAPSKIVASYTTTSGET+TSLLLTSGWW
Sbjct: 301 LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW 360

Query: 361 GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK 412
           GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILL DRAKRDDDRCRSKYGKYWK
Sbjct: 361 GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK 420

BLAST of Spg015094 vs. NCBI nr
Match: KAG6593601.1 (7-dehydrocholesterol reductase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 784.3 bits (2024), Expect = 5.3e-223
Identity = 373/434 (85.94%), Postives = 390/434 (89.86%), Query Frame = 0

Query: 1   MAEGSNTVHSPLLTYVSMLSLLTLCPPFVIFLWYTMVHTDGSVSQSWNYLYQNGLSGFIN 60
           MAEGSNTVHSPL+TY SMLSLLTLCPPFVIFLWYTMVH DGSVSQ+  YLY NGL GF++
Sbjct: 1   MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFVD 60

Query: 61  IWPPPSATACKIIGCYATFEAALQLLLPGKRVQGPVSPTGNQPVYKANGVAAYIVTLVTY 120
           IWPPPSATA K+IG YA FEAALQLLLPGK V GP+SPTGNQPVYKANGVAAY+VTLVTY
Sbjct: 61  IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY 120

Query: 121 IALWWYGI--QSTFYLDLGR---------------------VVPSSSDSGSSGNIIIDFY 180
           IALWW+GI   +  Y  LG                      V PSSSDSGSSGNIIIDFY
Sbjct: 121 IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY 180

Query: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALILVY 240
           WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTAL+L Y
Sbjct: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY 240

Query: 241 VSKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWLPSVYTSPGMYLVNHSVNLGTQLAVYI 300
           V+KFFWWEAGYWNTMDIAHDRAGFYICWGCLVW+PSVYTSPGMYLVNH V+LGTQLAVYI
Sbjct: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAVYI 300

Query: 301 LVAGILCIYINYDCDRQRQEFRRKSGKCLVWGKAPSKIVASYTTTSGETRTSLLLTSGWW 360
           LVAG+LCIYINYDCDRQRQEFRRK+GKCLVWGKAPSKIVASYTTTSGET+TSLLLTSGWW
Sbjct: 301 LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW 360

Query: 361 GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK 412
           GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILL DRAKRDDDRCRSKYGKYWK
Sbjct: 361 GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK 420

BLAST of Spg015094 vs. ExPASy Swiss-Prot
Match: Q9LDU6 (7-dehydrocholesterol reductase OS=Arabidopsis thaliana OX=3702 GN=DWF5 PE=1 SV=1)

HSP 1 Score: 719.5 bits (1856), Expect = 2.1e-206
Identity = 337/431 (78.19%), Postives = 369/431 (85.61%), Query Frame = 0

Query: 5   SNTVHSPLLTYVSMLSLLTLCPPFVIFLWYTMVHTDGSVSQSWNYLYQNGLSGFINIWPP 64
           + TVHSP++TY SMLSLL  CPPFVI LWYTMVH DGSV+Q++ + ++NG+ G INIWP 
Sbjct: 2   AETVHSPIVTYASMLSLLAFCPPFVILLWYTMVHQDGSVTQTFGFFWENGVQGLINIWPR 61

Query: 65  PSATACKIIGCYATFEAALQLLLPGKRVQGPVSPTGNQPVYKANGVAAYIVTLVTYIALW 124
           P+  A KII CY  FEA LQLLLPGKRV+GP+SP GN+PVYKANG+AAY VTL TY+ LW
Sbjct: 62  PTLIAWKIIFCYGAFEAILQLLLPGKRVEGPISPAGNRPVYKANGLAAYFVTLATYLGLW 121

Query: 125 WYGI--QSTFYLDL---------------------GRVVPSSSDSGSSGNIIIDFYWGME 184
           W+GI   +  Y  L                     G V PSSSDSGS GN+IIDFYWGME
Sbjct: 122 WFGIFNPAIVYDHLGEIFSALIFGSFIFCVLLYIKGHVAPSSSDSGSCGNLIIDFYWGME 181

Query: 185 LYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALILVYVSKF 244
           LYPRIGK FDIKVFTNCRFGMMSWAVLAVTYCIKQYE NG ++DSMLVNT L+LVYV+KF
Sbjct: 182 LYPRIGKSFDIKVFTNCRFGMMSWAVLAVTYCIKQYEINGKVSDSMLVNTILMLVYVTKF 241

Query: 245 FWWEAGYWNTMDIAHDRAGFYICWGCLVWLPSVYTSPGMYLVNHSVNLGTQLAVYILVAG 304
           FWWEAGYWNTMDIAHDRAGFYICWGCLVW+PSVYTSPGMYLVNH V LGTQLA+YILVAG
Sbjct: 242 FWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVELGTQLAIYILVAG 301

Query: 305 ILCIYINYDCDRQRQEFRRKSGKCLVWGKAPSKIVASYTTTSGETRTSLLLTSGWWGLAR 364
           ILCIYINYDCDRQRQEFRR +GKCLVWG+APSKIVASYTTTSGET+TSLLLTSGWWGLAR
Sbjct: 302 ILCIYINYDCDRQRQEFRRTNGKCLVWGRAPSKIVASYTTTSGETKTSLLLTSGWWGLAR 361

Query: 365 HFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWKLYCE 413
           HFHYVPEIL+AFFWTVPALF++FL YFYVIFLT+LLFDRAKRDDDRCRSKYGKYWKLYCE
Sbjct: 362 HFHYVPEILSAFFWTVPALFDNFLAYFYVIFLTLLLFDRAKRDDDRCRSKYGKYWKLYCE 421

BLAST of Spg015094 vs. ExPASy Swiss-Prot
Match: O88455 (7-dehydrocholesterol reductase OS=Mus musculus OX=10090 GN=Dhcr7 PE=1 SV=1)

HSP 1 Score: 273.9 bits (699), Expect = 3.0e-72
Identity = 155/438 (35.39%), Postives = 238/438 (54.34%), Query Frame = 0

Query: 16  VSMLSLLTLCPPFVIFLWYTMVHTDGSVSQSWNYL-YQNGLSGFINIW---PPPSATACK 75
           ++ +  L L  PF+++ ++ M     S S +   L    G +   +IW   PP +A A +
Sbjct: 36  LASIIFLLLFAPFIVY-YFIMACDQYSCSLTAPALDIATGHASLADIWAKTPPVTAKAAQ 95

Query: 76  IIGCYATFEAALQLLLP-----------GKRVQGPVSPTGNQPVYKANGVAAYIVTLV-- 135
           +   + +F+  L   LP           G   +G ++P G    Y+ NG+ A+++T +  
Sbjct: 96  LYALWVSFQVLLYSWLPDFCHRFLPGYVGGVQEGAITPAGVVNKYEVNGLQAWLITHILW 155

Query: 136 -----------------TYIALWW------YGIQSTFYLDLGRVVPSSS-DSGSSGNIII 195
                             +I L W      Y + STF +  G + P+S+ D   +GN   
Sbjct: 156 FVNAYLLSWFSPTIIFDNWIPLLWCANILGYAV-STFAMIKGYLFPTSAEDCKFTGNFFY 215

Query: 196 DFYWGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALI 255
           ++  G+E  PRIGK FD K+F N R G+++W ++ +++  KQ E  G + +SM++   L 
Sbjct: 216 NYMMGIEFNPRIGKWFDFKLFFNGRPGIVAWTLINLSFAAKQQELYGHVTNSMILVNVLQ 275

Query: 256 LVYVSKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWLPSVYTSPGMYLVNHSVNLGTQLA 315
            +YV  FFW E  Y  T+DI HD  G+Y+ WG  VWLP +YT  G+YLV H V L T  A
Sbjct: 276 AIYVLDFFWNETWYLKTIDICHDHFGWYLGWGDCVWLPYLYTLQGLYLVYHPVQLSTPNA 335

Query: 316 VYILVAGILCIYINYDCDRQRQEFRRKSGKCLVWGKAPSKIVASYTTTSGETRTSLLLTS 375
           + IL+ G++  YI    + Q+  FRR  G+CL+WGK P  I  SYT+  G    S LL S
Sbjct: 336 LGILLLGLVGYYIFRMTNHQKDLFRRTDGRCLIWGKKPKAIECSYTSADGLKHHSKLLVS 395

Query: 376 GWWGLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGK 413
           G+WG+ARHF+Y  +++ +  + +     H LPYFY+I++TILL  R  RD+ RC +KYG+
Sbjct: 396 GFWGVARHFNYTGDLMGSLAYCLACGGGHLLPYFYIIYMTILLTHRCLRDEHRCANKYGR 455

BLAST of Spg015094 vs. ExPASy Swiss-Prot
Match: Q9Z2Z8 (7-dehydrocholesterol reductase OS=Rattus norvegicus OX=10116 GN=Dhcr7 PE=1 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 5.2e-72
Identity = 156/438 (35.62%), Postives = 239/438 (54.57%), Query Frame = 0

Query: 16  VSMLSLLTLCPPFVIFLWYTMVHTDGSVSQSWNYL-YQNGLSGFINIW---PPPSATACK 75
           VS++ LL L  PF+++ ++ M     S S +   L    G +   +IW   PP +A A +
Sbjct: 37  VSVIFLL-LFAPFIVY-YFIMACDQYSCSLTAPILDVATGRASLADIWAKTPPVTAKAAQ 96

Query: 76  IIGCYATFEAALQLLLP-----------GKRVQGPVSPTGNQPVYKANGVAAYIVT---- 135
           +   + +F+  L   LP           G   +G ++P G    Y+ NG+ A+++T    
Sbjct: 97  LYALWVSFQVLLYSWLPDFCHRFLPGYVGGVQEGAITPAGIVNKYEVNGLQAWLITHFLW 156

Query: 136 -LVTYIALWW--------------------YGIQSTFYLDLGRVVPSSS-DSGSSGNIII 195
            +  Y+  W+                    Y + STF +  G + P+S+ D   +GN   
Sbjct: 157 FVNAYLLSWFSPTIIFDNWIPLLWCANILGYAV-STFAMIKGYLFPTSAEDCKFTGNFFY 216

Query: 196 DFYWGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALI 255
           ++  G+E  PRIGK FD K+F N R G+++W ++ +++  KQ E  G + +SM++   L 
Sbjct: 217 NYMMGIEFNPRIGKWFDFKLFFNGRPGIVAWTLINLSFAAKQQELYGHVTNSMILVNVLQ 276

Query: 256 LVYVSKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWLPSVYTSPGMYLVNHSVNLGTQLA 315
            +YV  FFW E  Y  T+DI HD  G+Y+ WG  VWLP +YT  G+YLV H V L T  A
Sbjct: 277 AIYVLDFFWNETWYLKTIDICHDHFGWYLGWGDCVWLPYLYTLQGLYLVYHPVQLSTPNA 336

Query: 316 VYILVAGILCIYINYDCDRQRQEFRRKSGKCLVWGKAPSKIVASYTTTSGETRTSLLLTS 375
           + +L+ G++  YI    + Q+  FRR  G CL+WGK P  I  SYT+  G    S LL S
Sbjct: 337 LGVLLLGLVGYYIFRMTNHQKDLFRRTDGHCLIWGKKPKAIECSYTSADGLKHRSKLLVS 396

Query: 376 GWWGLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGK 413
           G+WG+ARHF+Y  +++ +  + +     H LPYFY+I++TILL  R  RD+ RC +KYG+
Sbjct: 397 GFWGVARHFNYTGDLMGSLAYCLACGGGHLLPYFYIIYMTILLTHRCLRDEHRCANKYGR 456

BLAST of Spg015094 vs. ExPASy Swiss-Prot
Match: Q5E9J5 (7-dehydrocholesterol reductase OS=Bos taurus OX=9913 GN=DHCR7 PE=2 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 6.8e-72
Identity = 146/398 (36.68%), Postives = 220/398 (55.28%), Query Frame = 0

Query: 54  GLSGFINIW---PPPSATACKIIGCYATFEAALQLLLP-----------GKRVQGPVSPT 113
           G +   +IW   PP +A A +I   + T +  L +LLP           G   +G V+P 
Sbjct: 78  GRARLADIWARTPPVTAKAAQIYTAWVTLQVLLYMLLPDFCHKFLPGYVGGVQEGAVTPA 137

Query: 114 GNQPVYKANGVAAYIVTLVTYIA----LWWYG--------------------IQSTFYLD 173
           G    Y+ NG+ A+++T + + A    L W+                       STF + 
Sbjct: 138 GAVNKYEINGLQAWLLTHLLWFANAHLLGWFSPTIIFDNWIPLLWCANILGYTVSTFAMV 197

Query: 174 LGRVVPSSS-DSGSSGNIIIDFYWGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCI 233
            G + P+ + +   +GN   ++  G+E  PRIGK FD K+F N R G+++W ++ +++  
Sbjct: 198 KGYLFPTDARECKFTGNFFYNYMMGVEFNPRIGKWFDFKLFFNGRPGIVAWTLINLSFAA 257

Query: 234 KQYEKNGTIADSMLVNTALILVYVSKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWLPSV 293
           KQ E  G + +SM++   L  +YV  FFW E  Y  T+DI HD  G+Y+ WG  VWLP +
Sbjct: 258 KQQELYGHVTNSMVLVNILQAIYVLDFFWNETWYLKTIDICHDHFGWYLGWGDCVWLPYL 317

Query: 294 YTSPGMYLVNHSVNLGTQLAVYILVAGILCIYINYDCDRQRQEFRRKSGKCLVWGKAPSK 353
           YT  G+YLV H V L T  A+ +L+ G+L  YI    + Q+  FRR  G+CL+WG+ P  
Sbjct: 318 YTLQGLYLVYHPVQLPTYYALGVLLLGLLGYYIFRMTNHQKDLFRRTDGRCLIWGRKPKA 377

Query: 354 IVASYTTTSGETRTSLLLTSGWWGLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLT 413
           I  SYT+  G+   S LL SG+WG+ARHF+Y  +++ +  + +     H LPYFY+IF+ 
Sbjct: 378 IECSYTSADGQRHHSKLLVSGFWGVARHFNYTGDLMGSLAYCLACGGGHLLPYFYIIFMA 437

BLAST of Spg015094 vs. ExPASy Swiss-Prot
Match: Q9UBM7 (7-dehydrocholesterol reductase OS=Homo sapiens OX=9606 GN=DHCR7 PE=1 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 9.8e-71
Identity = 145/392 (36.99%), Postives = 217/392 (55.36%), Query Frame = 0

Query: 60  NIW---PPPSATACKIIGCYATFEAALQLLLP-----------GKRVQGPVSPTGNQPVY 119
           +IW   PP +  A ++   + TF+  L   LP           G   +G V+P G    Y
Sbjct: 84  DIWAKTPPITRKAAQLYTLWVTFQVLLYTSLPDFCHKFLPGYVGGIQEGAVTPAGVVNKY 143

Query: 120 KANGVAAYIVTLVTYIA--------------------LWWYGI----QSTFYLDLGRVVP 179
           + NG+ A+++T + + A                    LW   I     STF +  G   P
Sbjct: 144 QINGLQAWLLTHLLWFANAHLLSWFSPTIIFDNWIPLLWCANILGYAVSTFAMVKGYFFP 203

Query: 180 SSS-DSGSSGNIIIDFYWGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKN 239
           +S+ D   +GN   ++  G+E  PRIGK FD K+F N R G+++W ++ +++  KQ E +
Sbjct: 204 TSARDCKFTGNFFYNYMMGIEFNPRIGKWFDFKLFFNGRPGIVAWTLINLSFAAKQRELH 263

Query: 240 GTIADSMLVNTALILVYVSKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWLPSVYTSPGM 299
             + ++M++   L  +YV  FFW E  Y  T+DI HD  G+Y+ WG  VWLP +YT  G+
Sbjct: 264 SHVTNAMVLVNVLQAIYVIDFFWNETWYLKTIDICHDHFGWYLGWGDCVWLPYLYTLQGL 323

Query: 300 YLVNHSVNLGTQLAVYILVAGILCIYINYDCDRQRQEFRRKSGKCLVWGKAPSKIVASYT 359
           YLV H V L T  AV +L+ G++  YI    + Q+  FRR  G+CL+WG+ P  I  SYT
Sbjct: 324 YLVYHPVQLSTPHAVGVLLLGLVGYYIFRVANHQKDLFRRTDGRCLIWGRKPKVIECSYT 383

Query: 360 TTSGETRTSLLLTSGWWGLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDR 413
           +  G+   S LL SG+WG+ARHF+YV +++ +  + +     H LPYFY+I++ ILL  R
Sbjct: 384 SADGQRHHSKLLVSGFWGVARHFNYVGDLMGSLAYCLACGGGHLLPYFYIIYMAILLTHR 443

BLAST of Spg015094 vs. ExPASy TrEMBL
Match: A0A6J1D3V8 (7-dehydrocholesterol reductase OS=Momordica charantia OX=3673 GN=LOC111016744 PE=3 SV=1)

HSP 1 Score: 796.6 bits (2056), Expect = 5.0e-227
Identity = 376/435 (86.44%), Postives = 392/435 (90.11%), Query Frame = 0

Query: 1   MAEGSNTVHSPLLTYVSMLSLLTLCPPFVIFLWYTMVHTDGSVSQSWNYLYQNGLSGFIN 60
           MAEGS TVHSP+LTYVSMLSLL +CPPFVIFLWYTMVH DGSVSQ+WNYLY NG+ GF+N
Sbjct: 1   MAEGSTTVHSPILTYVSMLSLLIICPPFVIFLWYTMVHADGSVSQTWNYLYHNGVRGFMN 60

Query: 61  IWPPPSATACKIIGCYATFEAALQLLLPGKRVQGPVSPTGNQPVYKANGVAAYIVTLVTY 120
           IWPPPSATACKIIGCYA FEAALQLLLPG RVQGPVSPTGNQPVYKANGVAAYIVTLVTY
Sbjct: 61  IWPPPSATACKIIGCYAAFEAALQLLLPGNRVQGPVSPTGNQPVYKANGVAAYIVTLVTY 120

Query: 121 IALWWYGIQS-----------------------TFYLDLGRVVPSSSDSGSSGNIIIDFY 180
           IALWW+GI +                        F    G V PSSSDSGSSGNIIIDFY
Sbjct: 121 IALWWFGIFNPTIVYDHLGEIFSALIFGSLIFCIFLYIKGHVAPSSSDSGSSGNIIIDFY 180

Query: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALILVY 240
           WGMELYPRIGK+FDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTAL+LVY
Sbjct: 181 WGMELYPRIGKNFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLVY 240

Query: 241 VSKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWLPSVYTSPGMYLVNHSVNLGTQLAVYI 300
           V+KFFWWE+GYWNTMDIAHDRAGFYICWGCLVW+PSVYTSPGMYLVNH VNLGTQLAVYI
Sbjct: 241 VTKFFWWESGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVYI 300

Query: 301 LVAGILCIYINYDCDRQRQEFRRKSGKCLVWGKAPSKIVASYTTTSGETRTSLLLTSGWW 360
           LVAG+LCIYINYDCDRQRQEFRRK+GKCL+W KAPSKIVASYTTTSGET+TSLLLTSGWW
Sbjct: 301 LVAGLLCIYINYDCDRQRQEFRRKNGKCLIWRKAPSKIVASYTTTSGETKTSLLLTSGWW 360

Query: 361 GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK 413
           GLARHFHY PEILAAFFWTVPALFNHFLPYFYVIFLTILL DRAKRDDDRCRSKYGKYWK
Sbjct: 361 GLARHFHYAPEILAAFFWTVPALFNHFLPYFYVIFLTILLLDRAKRDDDRCRSKYGKYWK 420

BLAST of Spg015094 vs. ExPASy TrEMBL
Match: A0A0A0KC98 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G447780 PE=3 SV=1)

HSP 1 Score: 794.7 bits (2051), Expect = 1.9e-226
Identity = 377/435 (86.67%), Postives = 393/435 (90.34%), Query Frame = 0

Query: 1   MAEGSNTVHSPLLTYVSMLSLLTLCPPFVIFLWYTMVHTDGSVSQSWNYLYQNGLSGFIN 60
           MAEGSNTVHSP LTYVSMLSLLTLCPPFVI LWYTMVH DGSVSQ+WNYLYQNG  GFIN
Sbjct: 1   MAEGSNTVHSPFLTYVSMLSLLTLCPPFVILLWYTMVHADGSVSQTWNYLYQNGFEGFIN 60

Query: 61  IWPPPSATACKIIGCYATFEAALQLLLPGKRVQGPVSPTGNQPVYKANGVAAYIVTLVTY 120
           IWP PSATACKIIGCYA FEAALQLLLPGKRV GPVSPTGNQPVYKANGVAAYIVTL+TY
Sbjct: 61  IWPSPSATACKIIGCYAAFEAALQLLLPGKRVLGPVSPTGNQPVYKANGVAAYIVTLLTY 120

Query: 121 IALWWYGI---------QSTFYLDL--------------GRVVPSSSDSGSSGNIIIDFY 180
           +ALW +GI             Y  L              G V PSSSDSGSSGN+I+DFY
Sbjct: 121 VALWRFGIFDPAIVYDHLGEIYSTLIFGSFIFCILLYIKGHVAPSSSDSGSSGNVILDFY 180

Query: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALILVY 240
           WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGT+ADS+LVNTAL+LVY
Sbjct: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTLADSLLVNTALMLVY 240

Query: 241 VSKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWLPSVYTSPGMYLVNHSVNLGTQLAVYI 300
           V+KFFWWEAGYWNTMDIAHDRAGFYICWGCLVW+PSVYTSPGMYLVNH VNLGTQLAV I
Sbjct: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVSI 300

Query: 301 LVAGILCIYINYDCDRQRQEFRRKSGKCLVWGKAPSKIVASYTTTSGETRTSLLLTSGWW 360
           LVAGILCIYINYDCDRQRQEFRRK+GKCLVWGKAPSKIVA+YTTT+GETRTSLLLTSGWW
Sbjct: 301 LVAGILCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVATYTTTTGETRTSLLLTSGWW 360

Query: 361 GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK 413
           GLARHFHYVPEILAAFFWTVPALF+HFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK
Sbjct: 361 GLARHFHYVPEILAAFFWTVPALFHHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK 420

BLAST of Spg015094 vs. ExPASy TrEMBL
Match: A0A5A7UXH5 (7-dehydrocholesterol reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G001860 PE=3 SV=1)

HSP 1 Score: 792.3 bits (2045), Expect = 9.4e-226
Identity = 380/435 (87.36%), Postives = 392/435 (90.11%), Query Frame = 0

Query: 1   MAEGSNTVHSPLLTYVSMLSLLTLCPPFVIFLWYTMVHTDGSVSQSWNYLYQNGLSGFIN 60
           MAEGSNTVHSP LTYVSMLSLLTLCPPFVI LWYTMVH DGSVSQ+WNYLYQNG  GFIN
Sbjct: 1   MAEGSNTVHSPFLTYVSMLSLLTLCPPFVILLWYTMVHADGSVSQTWNYLYQNGFEGFIN 60

Query: 61  IWPPPSATACKIIGCYATFEAALQLLLPGKRVQGPVSPTGNQPVYKANGVAAYIVTLVTY 120
           IWP PSATACKIIGCYA FEAALQLLLPGKRV GPVSPTGNQPVYKANGVAAYIVTLVTY
Sbjct: 61  IWPSPSATACKIIGCYAAFEAALQLLLPGKRVLGPVSPTGNQPVYKANGVAAYIVTLVTY 120

Query: 121 IALWWYGI--QSTFYLDL---------------------GRVVPSSSDSGSSGNIIIDFY 180
           +ALW +GI   +  Y  L                     G V PSSSDSGSSGNII+DFY
Sbjct: 121 VALWRFGIFDPAIVYDHLGEIFSTLIFGSFIFCILLYIKGHVAPSSSDSGSSGNIILDFY 180

Query: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALILVY 240
           WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNG +ADSMLVNTAL+LVY
Sbjct: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGILADSMLVNTALMLVY 240

Query: 241 VSKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWLPSVYTSPGMYLVNHSVNLGTQLAVYI 300
           V+KFFWWEAGYWNTMDIAHDRAGFYICWGCLVW+PSVYTSPGMYLVNH VNLGTQLAV I
Sbjct: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVSI 300

Query: 301 LVAGILCIYINYDCDRQRQEFRRKSGKCLVWGKAPSKIVASYTTTSGETRTSLLLTSGWW 360
           LVAGILCIYINYDCDRQRQEFRRK+GK LVWGKAPSKIVASYTTTSGETRTSLLLTSGWW
Sbjct: 301 LVAGILCIYINYDCDRQRQEFRRKNGKRLVWGKAPSKIVASYTTTSGETRTSLLLTSGWW 360

Query: 361 GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK 413
           GLARHFHYVPEILAAFFWTVPALF+HFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK
Sbjct: 361 GLARHFHYVPEILAAFFWTVPALFHHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK 420

BLAST of Spg015094 vs. ExPASy TrEMBL
Match: A0A1S3CGG3 (7-dehydrocholesterol reductase OS=Cucumis melo OX=3656 GN=LOC103500620 PE=3 SV=1)

HSP 1 Score: 792.3 bits (2045), Expect = 9.4e-226
Identity = 380/435 (87.36%), Postives = 392/435 (90.11%), Query Frame = 0

Query: 1   MAEGSNTVHSPLLTYVSMLSLLTLCPPFVIFLWYTMVHTDGSVSQSWNYLYQNGLSGFIN 60
           MAEGSNTVHSP LTYVSMLSLLTLCPPFVI LWYTMVH DGSVSQ+WNYLYQNG  GFIN
Sbjct: 1   MAEGSNTVHSPFLTYVSMLSLLTLCPPFVILLWYTMVHADGSVSQTWNYLYQNGFEGFIN 60

Query: 61  IWPPPSATACKIIGCYATFEAALQLLLPGKRVQGPVSPTGNQPVYKANGVAAYIVTLVTY 120
           IWP PSATACKIIGCYA FEAALQLLLPGKRV GPVSPTGNQPVYKANGVAAYIVTLVTY
Sbjct: 61  IWPSPSATACKIIGCYAAFEAALQLLLPGKRVLGPVSPTGNQPVYKANGVAAYIVTLVTY 120

Query: 121 IALWWYGI--QSTFYLDL---------------------GRVVPSSSDSGSSGNIIIDFY 180
           +ALW +GI   +  Y  L                     G V PSSSDSGSSGNII+DFY
Sbjct: 121 VALWRFGIFDPAIVYDHLGEIFSTLIFGSFIFCILLYIKGHVAPSSSDSGSSGNIILDFY 180

Query: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALILVY 240
           WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNG +ADSMLVNTAL+LVY
Sbjct: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGILADSMLVNTALMLVY 240

Query: 241 VSKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWLPSVYTSPGMYLVNHSVNLGTQLAVYI 300
           V+KFFWWEAGYWNTMDIAHDRAGFYICWGCLVW+PSVYTSPGMYLVNH VNLGTQLAV I
Sbjct: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVSI 300

Query: 301 LVAGILCIYINYDCDRQRQEFRRKSGKCLVWGKAPSKIVASYTTTSGETRTSLLLTSGWW 360
           LVAGILCIYINYDCDRQRQEFRRK+GK LVWGKAPSKIVASYTTTSGETRTSLLLTSGWW
Sbjct: 301 LVAGILCIYINYDCDRQRQEFRRKNGKRLVWGKAPSKIVASYTTTSGETRTSLLLTSGWW 360

Query: 361 GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK 413
           GLARHFHYVPEILAAFFWTVPALF+HFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK
Sbjct: 361 GLARHFHYVPEILAAFFWTVPALFHHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK 420

BLAST of Spg015094 vs. ExPASy TrEMBL
Match: A0A6J1HK80 (7-dehydrocholesterol reductase OS=Cucurbita moschata OX=3662 GN=LOC111464317 PE=3 SV=1)

HSP 1 Score: 784.6 bits (2025), Expect = 2.0e-223
Identity = 375/434 (86.41%), Postives = 389/434 (89.63%), Query Frame = 0

Query: 1   MAEGSNTVHSPLLTYVSMLSLLTLCPPFVIFLWYTMVHTDGSVSQSWNYLYQNGLSGFIN 60
           MAEGSNTVHSPL+TY SMLSLLTLCPPFVIFLWYTMVH DGSVSQ+  YLY NGL G I+
Sbjct: 1   MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGLID 60

Query: 61  IWPPPSATACKIIGCYATFEAALQLLLPGKRVQGPVSPTGNQPVYKANGVAAYIVTLVTY 120
           IWPPPSATA K+IG YA FEAALQLLLPGK V GP+SPTGNQPVYKANGVAAY+VTLVTY
Sbjct: 61  IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY 120

Query: 121 IALWWYGI--QSTFYLDLGR---------------------VVPSSSDSGSSGNIIIDFY 180
           IALWW+GI   +  Y  LG                      V PSSSDSGSSGNIIIDFY
Sbjct: 121 IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY 180

Query: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALILVY 240
           WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTAL+L Y
Sbjct: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY 240

Query: 241 VSKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWLPSVYTSPGMYLVNHSVNLGTQLAVYI 300
           V+KFFWWEAGYWNTMDIAHDRAGFYICWGCLVW+PSVYTSPGMYLVNH VNLGTQLAVYI
Sbjct: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVYI 300

Query: 301 LVAGILCIYINYDCDRQRQEFRRKSGKCLVWGKAPSKIVASYTTTSGETRTSLLLTSGWW 360
           LVAG+LCIYINYDCDRQRQEFRRK+GKCLVWGKAPSKIVASYTTTSGET+TSLLLTSGWW
Sbjct: 301 LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW 360

Query: 361 GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK 412
           GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILL DRAKRDDDRCRSKYGKYWK
Sbjct: 361 GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK 420

BLAST of Spg015094 vs. TAIR 10
Match: AT1G50430.1 (Ergosterol biosynthesis ERG4/ERG24 family )

HSP 1 Score: 719.5 bits (1856), Expect = 1.5e-207
Identity = 337/431 (78.19%), Postives = 369/431 (85.61%), Query Frame = 0

Query: 5   SNTVHSPLLTYVSMLSLLTLCPPFVIFLWYTMVHTDGSVSQSWNYLYQNGLSGFINIWPP 64
           + TVHSP++TY SMLSLL  CPPFVI LWYTMVH DGSV+Q++ + ++NG+ G INIWP 
Sbjct: 2   AETVHSPIVTYASMLSLLAFCPPFVILLWYTMVHQDGSVTQTFGFFWENGVQGLINIWPR 61

Query: 65  PSATACKIIGCYATFEAALQLLLPGKRVQGPVSPTGNQPVYKANGVAAYIVTLVTYIALW 124
           P+  A KII CY  FEA LQLLLPGKRV+GP+SP GN+PVYKANG+AAY VTL TY+ LW
Sbjct: 62  PTLIAWKIIFCYGAFEAILQLLLPGKRVEGPISPAGNRPVYKANGLAAYFVTLATYLGLW 121

Query: 125 WYGI--QSTFYLDL---------------------GRVVPSSSDSGSSGNIIIDFYWGME 184
           W+GI   +  Y  L                     G V PSSSDSGS GN+IIDFYWGME
Sbjct: 122 WFGIFNPAIVYDHLGEIFSALIFGSFIFCVLLYIKGHVAPSSSDSGSCGNLIIDFYWGME 181

Query: 185 LYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALILVYVSKF 244
           LYPRIGK FDIKVFTNCRFGMMSWAVLAVTYCIKQYE NG ++DSMLVNT L+LVYV+KF
Sbjct: 182 LYPRIGKSFDIKVFTNCRFGMMSWAVLAVTYCIKQYEINGKVSDSMLVNTILMLVYVTKF 241

Query: 245 FWWEAGYWNTMDIAHDRAGFYICWGCLVWLPSVYTSPGMYLVNHSVNLGTQLAVYILVAG 304
           FWWEAGYWNTMDIAHDRAGFYICWGCLVW+PSVYTSPGMYLVNH V LGTQLA+YILVAG
Sbjct: 242 FWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVELGTQLAIYILVAG 301

Query: 305 ILCIYINYDCDRQRQEFRRKSGKCLVWGKAPSKIVASYTTTSGETRTSLLLTSGWWGLAR 364
           ILCIYINYDCDRQRQEFRR +GKCLVWG+APSKIVASYTTTSGET+TSLLLTSGWWGLAR
Sbjct: 302 ILCIYINYDCDRQRQEFRRTNGKCLVWGRAPSKIVASYTTTSGETKTSLLLTSGWWGLAR 361

Query: 365 HFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWKLYCE 413
           HFHYVPEIL+AFFWTVPALF++FL YFYVIFLT+LLFDRAKRDDDRCRSKYGKYWKLYCE
Sbjct: 362 HFHYVPEILSAFFWTVPALFDNFLAYFYVIFLTLLLFDRAKRDDDRCRSKYGKYWKLYCE 421

BLAST of Spg015094 vs. TAIR 10
Match: AT1G50430.2 (Ergosterol biosynthesis ERG4/ERG24 family )

HSP 1 Score: 711.4 bits (1835), Expect = 4.1e-205
Identity = 333/430 (77.44%), Postives = 364/430 (84.65%), Query Frame = 0

Query: 5   SNTVHSPLLTYVSMLSLLTLCPPFVIFLWYTMVHTDGSVSQSWNYLYQNGLSGFINIWPP 64
           + TVHSP++TY SMLSLL  CPPFVI LWYTMVH DGSV+Q++ + ++NG+ G INIWP 
Sbjct: 2   AETVHSPIVTYASMLSLLAFCPPFVILLWYTMVHQDGSVTQTFGFFWENGVQGLINIWPR 61

Query: 65  PSATACKIIGCYATFEAALQLLLPGKRVQGPVSPTGNQPVYKANGVAAYIVTLVTYIALW 124
           P+  A KII CY  FEA LQLLLPGKRV+GP+SP GN+PVYKANG+AAY VTL TY+ LW
Sbjct: 62  PTLIAWKIIFCYGAFEAILQLLLPGKRVEGPISPAGNRPVYKANGLAAYFVTLATYLGLW 121

Query: 125 WYGIQSTFYLD----------------------LGRVVPSSSDSGSSGNIIIDFYWGMEL 184
           +         D                       G V PSSSDSGS GN+IIDFYWGMEL
Sbjct: 122 FGIFNPAIVYDHLGEIFSALIFGSFIFCVLLYIKGHVAPSSSDSGSCGNLIIDFYWGMEL 181

Query: 185 YPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALILVYVSKFF 244
           YPRIGK FDIKVFTNCRFGMMSWAVLAVTYCIKQYE NG ++DSMLVNT L+LVYV+KFF
Sbjct: 182 YPRIGKSFDIKVFTNCRFGMMSWAVLAVTYCIKQYEINGKVSDSMLVNTILMLVYVTKFF 241

Query: 245 WWEAGYWNTMDIAHDRAGFYICWGCLVWLPSVYTSPGMYLVNHSVNLGTQLAVYILVAGI 304
           WWEAGYWNTMDIAHDRAGFYICWGCLVW+PSVYTSPGMYLVNH V LGTQLA+YILVAGI
Sbjct: 242 WWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVELGTQLAIYILVAGI 301

Query: 305 LCIYINYDCDRQRQEFRRKSGKCLVWGKAPSKIVASYTTTSGETRTSLLLTSGWWGLARH 364
           LCIYINYDCDRQRQEFRR +GKCLVWG+APSKIVASYTTTSGET+TSLLLTSGWWGLARH
Sbjct: 302 LCIYINYDCDRQRQEFRRTNGKCLVWGRAPSKIVASYTTTSGETKTSLLLTSGWWGLARH 361

Query: 365 FHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWKLYCEK 413
           FHYVPEIL+AFFWTVPALF++FL YFYVIFLT+LLFDRAKRDDDRCRSKYGKYWKLYCEK
Sbjct: 362 FHYVPEILSAFFWTVPALFDNFLAYFYVIFLTLLLFDRAKRDDDRCRSKYGKYWKLYCEK 421

BLAST of Spg015094 vs. TAIR 10
Match: AT3G52940.1 (Ergosterol biosynthesis ERG4/ERG24 family )

HSP 1 Score: 161.0 bits (406), Expect = 2.0e-39
Identity = 113/378 (29.89%), Postives = 196/378 (51.85%), Query Frame = 0

Query: 65  PSATACKIIGCYATFEAALQLLLPGKRVQGPVSPTGNQPVYKANGVAAYIVT-------- 124
           PS  +  ++  Y  + A    +LPGK ++G +   G+Q  Y+ NG+ A I+         
Sbjct: 12  PSLQSVYVLVFYFVYLAVAGEILPGKVIRGVLLSDGSQLRYRCNGLLALILLVAILGICA 71

Query: 125 ---LVTYIALWWYGIQ---STFY------LDLGRVVPSSSDSGS------SGNIIIDFYW 184
              +V+ + +   G++   +TF       L L     SSS+ GS      SGN++ D+++
Sbjct: 72  KLGIVSPLVVADRGLELLSATFIFCVLVTLALYVTGRSSSNKGSSLKPHVSGNLVHDWWF 131

Query: 185 GMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALILVYV 244
           G++L P+     D+K F   R GMM W ++ ++   K  + +G+++ SM++      +Y+
Sbjct: 132 GIQLNPQF-MSIDLKFFF-VRAGMMGWLLINLSILAKSVQ-DGSLSQSMILYQIFCALYI 191

Query: 245 SKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWLPSVYTSPGMYLVNHSVNLGTQLAVYIL 304
             +F  E    +T DI  +R GF + +G L+W+P  ++  G +L+++ V    +L V  +
Sbjct: 192 LDYFVHEEYMTSTWDIIAERLGFMLVFGDLLWIPFTFSIQGWWLLHNKV----ELTVPAI 251

Query: 305 VAGILCIYINY----DCDRQRQEFRRKSGKCLVWGKAPSKIVASYTTTSGETRTSLLLTS 364
           V   L   I Y      ++Q+  F +K+ K  +WGK P           G+     LL S
Sbjct: 252 VVNCLVFLIGYMVFRGANKQKHIF-KKNPKTPIWGKPP-------VVVGGK-----LLVS 311

Query: 365 GWWGLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGK 413
           G+WG+ARH +Y+ +++ A  +++P   +  +PYFY I+L ILL  R +RD+ RC  KY +
Sbjct: 312 GYWGIARHCNYLGDLMLALSFSLPCGISSPVPYFYPIYLLILLIWRERRDEVRCAEKYKE 369

BLAST of Spg015094 vs. TAIR 10
Match: AT3G52940.2 (Ergosterol biosynthesis ERG4/ERG24 family )

HSP 1 Score: 76.6 bits (187), Expect = 5.1e-14
Identity = 59/218 (27.06%), Postives = 110/218 (50.46%), Query Frame = 0

Query: 65  PSATACKIIGCYATFEAALQLLLPGKRVQGPVSPTGNQPVYKANGVAAYIVT-------- 124
           PS  +  ++  Y  + A    +LPGK ++G +   G+Q  Y+ NG+ A I+         
Sbjct: 12  PSLQSVYVLVFYFVYLAVAGEILPGKVIRGVLLSDGSQLRYRCNGLLALILLVAILGICA 71

Query: 125 ---LVTYIALWWYGIQ---STFY------LDLGRVVPSSSDSGS------SGNIIIDFYW 184
              +V+ + +   G++   +TF       L L     SSS+ GS      SGN++ D+++
Sbjct: 72  KLGIVSPLVVADRGLELLSATFIFCVLVTLALYVTGRSSSNKGSSLKPHVSGNLVHDWWF 131

Query: 185 GMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALILVYV 244
           G++L P+     D+K F   R GMM W ++ ++   K  + +G+++ SM++      +Y+
Sbjct: 132 GIQLNPQF-MSIDLKFFF-VRAGMMGWLLINLSILAKSVQ-DGSLSQSMILYQIFCALYI 191

Query: 245 SKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWLPSVYT 257
             +F  E    +T DI  +R GF + +G L+W+P  ++
Sbjct: 192 LDYFVHEEYMTSTWDIIAERLGFMLVFGDLLWIPFTFS 226

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022147941.11.0e-22686.447-dehydrocholesterol reductase [Momordica charantia][more]
XP_011659586.13.9e-22686.677-dehydrocholesterol reductase isoform X1 [Cucumis sativus] >KGN45416.1 hypothet... [more]
XP_008462206.11.9e-22587.36PREDICTED: 7-dehydrocholesterol reductase [Cucumis melo] >KAA0059337.1 7-dehydro... [more]
XP_022964233.14.0e-22386.417-dehydrocholesterol reductase [Cucurbita moschata][more]
KAG6593601.15.3e-22385.947-dehydrocholesterol reductase, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q9LDU62.1e-20678.197-dehydrocholesterol reductase OS=Arabidopsis thaliana OX=3702 GN=DWF5 PE=1 SV=1[more]
O884553.0e-7235.397-dehydrocholesterol reductase OS=Mus musculus OX=10090 GN=Dhcr7 PE=1 SV=1[more]
Q9Z2Z85.2e-7235.627-dehydrocholesterol reductase OS=Rattus norvegicus OX=10116 GN=Dhcr7 PE=1 SV=1[more]
Q5E9J56.8e-7236.687-dehydrocholesterol reductase OS=Bos taurus OX=9913 GN=DHCR7 PE=2 SV=1[more]
Q9UBM79.8e-7136.997-dehydrocholesterol reductase OS=Homo sapiens OX=9606 GN=DHCR7 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1D3V85.0e-22786.447-dehydrocholesterol reductase OS=Momordica charantia OX=3673 GN=LOC111016744 PE... [more]
A0A0A0KC981.9e-22686.67Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G447780 PE=3 SV=1[more]
A0A5A7UXH59.4e-22687.367-dehydrocholesterol reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3CGG39.4e-22687.367-dehydrocholesterol reductase OS=Cucumis melo OX=3656 GN=LOC103500620 PE=3 SV=1[more]
A0A6J1HK802.0e-22386.417-dehydrocholesterol reductase OS=Cucurbita moschata OX=3662 GN=LOC111464317 PE=... [more]
Match NameE-valueIdentityDescription
AT1G50430.11.5e-20778.19Ergosterol biosynthesis ERG4/ERG24 family [more]
AT1G50430.24.1e-20577.44Ergosterol biosynthesis ERG4/ERG24 family [more]
AT3G52940.12.0e-3929.89Ergosterol biosynthesis ERG4/ERG24 family [more]
AT3G52940.25.1e-1427.06Ergosterol biosynthesis ERG4/ERG24 family [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001171Ergosterol biosynthesis ERG4/ERG24PFAMPF01222ERG4_ERG24coord: 146..412
e-value: 2.0E-68
score: 231.2
NoneNo IPR availableGENE3D1.20.120.1630coord: 200..411
e-value: 2.1E-17
score: 65.5
NoneNo IPR availablePANTHERPTHR21257:SF537-DEHYDROCHOLESTEROL REDUCTASEcoord: 136..412
coord: 6..130
NoneNo IPR availablePANTHERPTHR21257DELTA(14)-STEROL REDUCTASEcoord: 136..412
coord: 6..130
IPR018083Sterol reductase, conserved sitePROSITEPS01017STEROL_REDUCT_1coord: 150..165
IPR018083Sterol reductase, conserved sitePROSITEPS01018STEROL_REDUCT_2coord: 376..399

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg015094.1Spg015094.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016132 brassinosteroid biosynthetic process
biological_process GO:0016126 sterol biosynthetic process
cellular_component GO:0030176 integral component of endoplasmic reticulum membrane
cellular_component GO:0016020 membrane
molecular_function GO:0016628 oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor
molecular_function GO:0009918 sterol delta7 reductase activity