Spg015060 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg015060
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionDUF4283 domain-containing protein
Locationscaffold3: 31733801 .. 31736380 (+)
RNA-Seq ExpressionSpg015060
SyntenySpg015060
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTTCGAGATCAGCCCGTAAGTGCGTAGCTGAGAGGAAACTGTTTGAGATAGACTATATCAAAGAGAGGAACAAGAGAGCAGTTAAGATTTCTGAGAAAAACCACGCTCTGCGGTTCGAGATTATTCTGGAAGTTAAACACGCAATTTGGATAGCAGATGTAGTCGATGACTTGCTCATTACCCCCATAAGCCAAAAATTTTTTAGAAAGACTTCGTGCGAAAACGGTTTCATTTGGTTTCAAAAGGTGTCCAACAAGAGAGGGGTTTTTGCAGAAATATCGAAGGTGACTTCATCTAGAAGAAGAGATAATCTGATTATTCCCCAGGGAGAAGACTCTACAGGATGGAAAGCTTTCCTCGCTATGGTTAACGACTTCTTCAATGGTAAGGATAAGTAGCAGAAAGTTACGAAGGCCGACCGAAGACCCCTTCAGTCCTATGCTGAAGCTGTGAAAAATCAGATCAAGGATGTTAGTCTGAATCCCATAAATCGCACGAGATCTTCGAGACCTTAAAGAGGCAGGTCGGCCGATCCCCGGGCCAGAGGGGAGAAGTTAAGCCCTATAACCCCCCCCCCGGAAAGTCAGTGAGTGTCGAAGGATCAACTGGGATCAAACTCTGGTTATTACGAGAAGATGCTTTCATGATGAATGGAGTAAAATCTTCGACACTCTTCGTAATGCTTTTCAGAAGGATCTAATCATCAACCCTTTCCACCCGGATAAGGCCCTCCTGAAGTGCCCGGACCCAGACTTTGCTAGAATCGTTGTTCATAATAAAGGTTGGTCAGTAATAGGGAACTTCACCCTGAAATTTGAATATTGGAACTATAAGTTGCATGGTAGAATTAATGTAGTTCCTTCTTATGGCAGCTGGCTGAGATTCCAGAACATTCCCCTGCAGAATTGGTGTCTTGACACTTTTAAAGCGATAGGGGACGTGTATGGAGGGTTTATTGAATGCGATGATAAATGCTTGTCTTTAGTTGGTTGTATGGAAGTCGTTATTCGCGTAAGAGACAATTATTGTGGTTTTATTCCAGCCGATTTTGAGCTGATGCAGGCAGACGGCTCAGTTTCGATCGCCCAAGTGGTTACTTTTGAAGATCCTCAACTCCTGGAAAGTAGACGAGTTTACATCCATGGAGGTTTTTCCAGTGAAGCAGCTAGGGTTTCCTTTGGGGAAGACGAAGGTAGTGACGAGCTTTGCCCAGTGGATAAAGCCCGTATGGAAAACAGGTCGGACAGGTTTCAGATCCAGGCGGCTCTAAACCAGGCTGGGGATGTCAGTATAAGCTACAAAAGGGGATTTCAAAGTTTTGAAAAGGATAAAGCTGAAAATATGACTTCTAGAAAGGGGGTGTCAGAAGAAAAGATGAGGCCCAGAGTGGGGAGTAGCAGTACCAGCGAAAAAGAGGAAAATAATACAAATGAACCAAGGAAGGCAGTGGGGCCCACGCGCGAGAAAAAGCAGGTTTGGAGAGAGAAAGACTTGTCTGATAAAGGAGTCTCCTTTGCCTTAAAATCTGAAGTTAGAACTTTCGTAAAAAAAGGTAATATTTGCTCCACGGTGGTTAATAATGAAATCAAGGAAGTTGATCCCAGTGACAAACCGTTAGATACTTTTGATATCGAAGGTTCCTTGATGAGTGAGTCAAGTTAGGAAGATGACTATCAACACTGTGAGGGTCATAGGCAGGCGTGGGATCAAGAGGAGGCCATAGAGGACATAGCAGCCATGTTTGCGAACGAAGAAGTCGAATGCCTAGGTCAAAACCGAAAAGATAAGATGGCCATAGTAGTTAGCGATTCTCAGGAACCTCTGATAGAAGCAAATCCGATTAATACTGAAGAGGATGATAGAAGGGAACCCTCGAGTCCTTGTGGATCTGGGCAGAAATCTGGTAGTGTAGAATCGGGGTCAAAGCTCGCAATTCCGATATTAAAGGAAACGATAGAAATCCTGTGGCAAAATGGGCTCTGTATTAGGCCAATTCCAAAGAAGATAGGAGGTGGGGCGAGCAAAGGCAAAAGTAAGAAAAATCCGATGAAAAGGGAGATAAAAGGGCTTATAAATACTTGGGAAAAATCGATGGAGAATTTTATTTCCCCATCTCAGGGTTGCGACGTTGCTCAATGAAGATTCTCTCATGGAATGTCAGGGGGTTGGGGGATGCGGATAAAAGAAGATTGGTTAAGGAGTTAGTTAGGAGTAGCGGCCCATATATTGTGCTTATTCAGGAGTCTAAATTGGGTTTTGTCCATAGGCACATTGTCAAGCAAGTGTGGAGCTCTCGTTTTATAAGTTGGTCTTCCTTTAATTCTGAAGGGTCTTCAGGTGGGATTCTGGTGTTGTGGAAGGAGAAAGAGGTCAAGGCAGTGGATGTGGTTGTGGGGAGTTTCACAGTGTCTGTCCTGTTTGAGCTTGAGAACGCCAAGAAATGCTGGATTACCGCCGTGTATGGTCCTAGTAGATATAAAGAAAGACCTTTGTTTTGGCGTGAGTTATATGATCTGAATGGCTTATGCAGCGGGGCTTGGTGTCTAGGGGGATTTCAACGTGGTCAGAAGAATTGA

mRNA sequence

ATGACTTCGAGATCAGCCCGTAAGTGCGTAGCTGAGAGGAAACTGTTTGAGATAGACTATATCAAAGAGAGGAACAAGAGAGCAGTTAAGATTTCTGAGAAAAACCACGCTCTGCGGTTCGAGATTATTCTGGAAGTTAAACACGCAATTTGGATAGCAGATGTAGTCGATGACTTGCTCATTACCCCCATAAGCCAAAAATTTTTTAGAAAGACTTCGTGCGAAAACGGTTTCATTTGGTTTCAAAAGGTGTCCAACAAGAGAGGGGTTTTTGCAGAAATATCGAAGGTGACTTCATCTAGAAGAAGAGATAATCTGATTATTCCCCAGGGAGAAGACTCTACAGGATGGAAAGCTTTCCTCGCTATGGTTAACGACTTCTTCAATGTCAGTGAGTGTCGAAGGATCAACTGGGATCAAACTCTGGTTATTACGAGAAGATGCTTTCATGATGAATGGAGTAAAATCTTCGACACTCTTCGTAATGCTTTTCAGAAGGATCTAATCATCAACCCTTTCCACCCGGATAAGGCCCTCCTGAAGTGCCCGGACCCAGACTTTGCTAGAATCGTTGTTCATAATAAAGGTTGGTCAGTAATAGGGAACTTCACCCTGAAATTTGAATATTGGAACTATAAGTTGCATGGTAGAATTAATGTAGTTCCTTCTTATGGCAGCTGGCTGAGATTCCAGAACATTCCCCTGCAGAATTGGTGTCTTGACACTTTTAAAGCGATAGGGGACGTGTATGGAGGGTTTATTGAATGCGATGATAAATGCTTGTCTTTAGTTGGTTGTATGGAAGTCGTTATTCGCGTAAGAGACAATTATTGTGGTTTTATTCCAGCCGATTTTGAGCTGATGCAGGCAGACGGCTCAGTTTCGATCGCCCAAGTGGTTACTTTTGAAGATCCTCAACTCCTGGAAAGTAGACGAGTTTACATCCATGGAGGTTTTTCCAGTGAAGCAGCTAGGGTTTCCTTTGGGGAAGACGAAGGTAGTGACGAGCTTTGCCCAGTGGATAAAGCCCGTATGGAAAACAGGTCGGACAGGTTTCAGATCCAGGCGGCTCTAAACCAGGCTGGGGATGTCAGTATAAGCTACAAAAGGGGATTTCAAAGTTTTGAAAAGGATAAAGCTGAAAATATGACTTCTAGAAAGGGGGTGTCAGAAGAAAAGATGAGGCCCAGAGTGGGGAGTAGCAGTACCAGCGAAAAAGAGGAAAATAATACAAATGAACCAAGGAAGGCAGTGGGGCCCACGCGCGAGAAAAAGCAGGTTTGGAGAGAGAAAGACTTGTCTGATAAAGGAGTCTCCTTTGCCTTAAAATCTGAAGCGTGGGATCAAGAGGAGGCCATAGAGGACATAGCAGCCATGTTTGCGAACGAAGAAGTCGAATGCCTAGGTCAAAACCGAAAAGATAAGATGGCCATAGTAGTTAGCGATTCTCAGGAACCTCTGATAGAAGCAAATCCGATTAATACTGAAGAGGATGATAGAAGGGAACCCTCGAGTCCTTGTGGATCTGGGCAGAAATCTGGTAGTGTAGAATCGGGGTCAAAGCTCGCAATTCCGATATTAAAGGAAACGATAGAAATCCTGTGGCAAAATGGGCTCTGTATTAGGCCAATTCCAAAGAAGATAGGAGGTGGGGCGAGCAAAGGCAAAAGGTTGCGACGTTGCTCAATGAAGATTCTCTCATGGAATGTCAGGGGGTTGGGGGATGCGGATAAAAGAAGATTGGTTAAGGAGTTAGTTAGGAGTAGCGGCCCATATATTGTGCTTATTCAGGAGTCTAAATTGGGTTTTGTCCATAGGCACATTGTCAAGCAAGTGTGGAGCTCTCGTTTTATAAGTTGGTCTTCCTTTAATTCTGAAGGGTCTTCAGGTGGGATTCTGGTGTTGTGGAAGGAGAAAGAGGTCAAGGCAGTGGATGTGGTTGTGGGGAGTTTCACAGTGTCTGTCCTGTTTGAGCTTGAGAACGCCAAGAAATGCTGGATTACCGCCGTGTATGGTCCTAGTAGATATAAAGAAAGACCTTTGTTTTGGCGTGAGTTATATGATCTGAATGGCTTATGCAGCGGGGCTTGGTGTCTAGGGGGATTTCAACGTGGTCAGAAGAATTGA

Coding sequence (CDS)

ATGACTTCGAGATCAGCCCGTAAGTGCGTAGCTGAGAGGAAACTGTTTGAGATAGACTATATCAAAGAGAGGAACAAGAGAGCAGTTAAGATTTCTGAGAAAAACCACGCTCTGCGGTTCGAGATTATTCTGGAAGTTAAACACGCAATTTGGATAGCAGATGTAGTCGATGACTTGCTCATTACCCCCATAAGCCAAAAATTTTTTAGAAAGACTTCGTGCGAAAACGGTTTCATTTGGTTTCAAAAGGTGTCCAACAAGAGAGGGGTTTTTGCAGAAATATCGAAGGTGACTTCATCTAGAAGAAGAGATAATCTGATTATTCCCCAGGGAGAAGACTCTACAGGATGGAAAGCTTTCCTCGCTATGGTTAACGACTTCTTCAATGTCAGTGAGTGTCGAAGGATCAACTGGGATCAAACTCTGGTTATTACGAGAAGATGCTTTCATGATGAATGGAGTAAAATCTTCGACACTCTTCGTAATGCTTTTCAGAAGGATCTAATCATCAACCCTTTCCACCCGGATAAGGCCCTCCTGAAGTGCCCGGACCCAGACTTTGCTAGAATCGTTGTTCATAATAAAGGTTGGTCAGTAATAGGGAACTTCACCCTGAAATTTGAATATTGGAACTATAAGTTGCATGGTAGAATTAATGTAGTTCCTTCTTATGGCAGCTGGCTGAGATTCCAGAACATTCCCCTGCAGAATTGGTGTCTTGACACTTTTAAAGCGATAGGGGACGTGTATGGAGGGTTTATTGAATGCGATGATAAATGCTTGTCTTTAGTTGGTTGTATGGAAGTCGTTATTCGCGTAAGAGACAATTATTGTGGTTTTATTCCAGCCGATTTTGAGCTGATGCAGGCAGACGGCTCAGTTTCGATCGCCCAAGTGGTTACTTTTGAAGATCCTCAACTCCTGGAAAGTAGACGAGTTTACATCCATGGAGGTTTTTCCAGTGAAGCAGCTAGGGTTTCCTTTGGGGAAGACGAAGGTAGTGACGAGCTTTGCCCAGTGGATAAAGCCCGTATGGAAAACAGGTCGGACAGGTTTCAGATCCAGGCGGCTCTAAACCAGGCTGGGGATGTCAGTATAAGCTACAAAAGGGGATTTCAAAGTTTTGAAAAGGATAAAGCTGAAAATATGACTTCTAGAAAGGGGGTGTCAGAAGAAAAGATGAGGCCCAGAGTGGGGAGTAGCAGTACCAGCGAAAAAGAGGAAAATAATACAAATGAACCAAGGAAGGCAGTGGGGCCCACGCGCGAGAAAAAGCAGGTTTGGAGAGAGAAAGACTTGTCTGATAAAGGAGTCTCCTTTGCCTTAAAATCTGAAGCGTGGGATCAAGAGGAGGCCATAGAGGACATAGCAGCCATGTTTGCGAACGAAGAAGTCGAATGCCTAGGTCAAAACCGAAAAGATAAGATGGCCATAGTAGTTAGCGATTCTCAGGAACCTCTGATAGAAGCAAATCCGATTAATACTGAAGAGGATGATAGAAGGGAACCCTCGAGTCCTTGTGGATCTGGGCAGAAATCTGGTAGTGTAGAATCGGGGTCAAAGCTCGCAATTCCGATATTAAAGGAAACGATAGAAATCCTGTGGCAAAATGGGCTCTGTATTAGGCCAATTCCAAAGAAGATAGGAGGTGGGGCGAGCAAAGGCAAAAGGTTGCGACGTTGCTCAATGAAGATTCTCTCATGGAATGTCAGGGGGTTGGGGGATGCGGATAAAAGAAGATTGGTTAAGGAGTTAGTTAGGAGTAGCGGCCCATATATTGTGCTTATTCAGGAGTCTAAATTGGGTTTTGTCCATAGGCACATTGTCAAGCAAGTGTGGAGCTCTCGTTTTATAAGTTGGTCTTCCTTTAATTCTGAAGGGTCTTCAGGTGGGATTCTGGTGTTGTGGAAGGAGAAAGAGGTCAAGGCAGTGGATGTGGTTGTGGGGAGTTTCACAGTGTCTGTCCTGTTTGAGCTTGAGAACGCCAAGAAATGCTGGATTACCGCCGTGTATGGTCCTAGTAGATATAAAGAAAGACCTTTGTTTTGGCGTGAGTTATATGATCTGAATGGCTTATGCAGCGGGGCTTGGTGTCTAGGGGGATTTCAACGTGGTCAGAAGAATTGA

Protein sequence

MTSRSARKCVAERKLFEIDYIKERNKRAVKISEKNHALRFEIILEVKHAIWIADVVDDLLITPISQKFFRKTSCENGFIWFQKVSNKRGVFAEISKVTSSRRRDNLIIPQGEDSTGWKAFLAMVNDFFNVSECRRINWDQTLVITRRCFHDEWSKIFDTLRNAFQKDLIINPFHPDKALLKCPDPDFARIVVHNKGWSVIGNFTLKFEYWNYKLHGRINVVPSYGSWLRFQNIPLQNWCLDTFKAIGDVYGGFIECDDKCLSLVGCMEVVIRVRDNYCGFIPADFELMQADGSVSIAQVVTFEDPQLLESRRVYIHGGFSSEAARVSFGEDEGSDELCPVDKARMENRSDRFQIQAALNQAGDVSISYKRGFQSFEKDKAENMTSRKGVSEEKMRPRVGSSSTSEKEENNTNEPRKAVGPTREKKQVWREKDLSDKGVSFALKSEAWDQEEAIEDIAAMFANEEVECLGQNRKDKMAIVVSDSQEPLIEANPINTEEDDRREPSSPCGSGQKSGSVESGSKLAIPILKETIEILWQNGLCIRPIPKKIGGGASKGKRLRRCSMKILSWNVRGLGDADKRRLVKELVRSSGPYIVLIQESKLGFVHRHIVKQVWSSRFISWSSFNSEGSSGGILVLWKEKEVKAVDVVVGSFTVSVLFELENAKKCWITAVYGPSRYKERPLFWRELYDLNGLCSGAWCLGGFQRGQKN
Homology
BLAST of Spg015060 vs. NCBI nr
Match: TYJ99315.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 232.3 bits (591), Expect = 1.3e-56
Identity = 198/819 (24.18%), Postives = 339/819 (41.39%), Query Frame = 0

Query: 7   RKCVAERKLFEIDYIKERNKRAVKISEKNHALRFEIILEVKHAIWIADVVDDLLITPISQ 66
           R C  ERK F +   K        ++E      F I +  +   WI   +  L+ TP + 
Sbjct: 63  RSCKVERKEFVLHLDKYSKHTHYWLTETGAHKAFSIEVSPRDLDWIRCTLKSLIATPNTN 122

Query: 67  KFFRKTSCENGFIWFQKVSNKRGVFAEISKVTSSRRRDNLIIPQGEDSTGWKAFLAMVN- 126
           +FF +T      IW +K  N +G  AEI +V    R+  +++P+G D +GW +FL+M+  
Sbjct: 123 RFFLETRDSEQRIWIRKTRNSKGCTAEIFRVDQKNRKSCILVPEGPDKSGWVSFLSMITP 182

Query: 127 --------------------------DFFNVSECRRIN---------------------- 186
                                     D+   S  + +                       
Sbjct: 183 KVEVKAKTRPTFLPRTSPDCRLSPPIDYHKRSYAKAVTEGRPFATSDSSDSYDSSDSSHS 242

Query: 187 -------------WDQTLVITRRCFHDEWSKIFDTLRNAFQKDLIINPFHPDKALLKCPD 246
                         + T+VI RR FHD+W KI   LR   ++    N FH +KAL+    
Sbjct: 243 SSNSFCDSPSSDLLENTVVIVRRFFHDDWHKILQNLRKQTEESFTYNAFHAEKALVHFSS 302

Query: 247 PDFARIVVHNKGWSVIGNFTLKFEYWNYKLHGRINVVPSYGSWLRFQNIPLQNWCLDTFK 306
              A ++  NKGWS +G ++++FE W+   H    ++PSYG W  F+ IPL  W + TF+
Sbjct: 303 NIPANLLCQNKGWSTVGKYSVRFEKWSPVYHATPKLIPSYGGWTTFRGIPLHLWNMMTFQ 362

Query: 307 AIGDVYGGFIECDDKCLSLVGCMEVVIRVRDNYCGFIPADFELMQADGSVSIAQVVTFED 366
            IG    G I+  ++  S    +E  I+VR NY GF+PA+  +   +G+    QVVT  +
Sbjct: 363 QIGKACEGLIKVAEETRSAKNLIEARIKVRYNYSGFLPANVRIFDNEGNKFFVQVVTHPE 422

Query: 367 PQLLESRRVYIHGGFSSEAARVSFGE---------DEGSDELCP-----VDKARMENRSD 426
            + L  R V +HG F  +AA  SF +          EGS+ + P         R  +  D
Sbjct: 423 GKWLIERNVRLHGTFKRQAA-ASFDDFNPESEQFFFEGSEAISPDFLSTSSDGRKSSTPD 482

Query: 427 R-------------------FQIQAALNQAGDVSISYKR-----------GFQSFEKDKA 486
           +                   F  +  +N +   + + K            G     K K 
Sbjct: 483 QPSALKSVIIKPDRNATLPSFLNEELVNDSNLHATANKSKLEILSGISNDGVLDKGKQKV 542

Query: 487 E-NMTSRKGVSEEKMRPRVGSSSTSEKEE--NNTNEPRK---AVGPTREKKQVWREKDLS 546
           +  +     ++ +K + +V  +S S K    N  + P     ++    +K++V RE+ + 
Sbjct: 543 DIQLQPNSALNLDKSKRKVSFNSPSNKTNIFNPDSAPANHSPSLNSPEKKQKVSRERSIK 602

Query: 547 DKGVSFALKSEAWDQEEAIEDIAAMFANEEVECLGQNR---KDKMAIVVSDSQEPLIEAN 606
            K  S    S+A +Q + +      F  + ++ +  +R   K  +++ V     P ++ N
Sbjct: 603 KKSSSTQPNSKA-NQNKGV------FITQPIQIVAHDRDAAKKGLSLTVDLGDLPALDPN 662

Query: 607 PINTEEDDRREPSSPCGSGQKSGSVESGSKLAIPILKETIEILWQNGLCIRPIPKKIGGG 666
              + ED     ++       +  V    ++ +P+ + +      N    + + K+    
Sbjct: 663 --KSLEDHHNSDNAEVVDITNTEVVPETPEMKMPVNENSNSSSEANYRKPKHVHKRKYYY 722

Query: 667 ASKGKRLR-----RCSMKILSW----NVRGLGDADKRRLVKELVRSSGPYIVLIQESKLG 702
             K ++ +         +++SW     ++   D D          +S   ++    S L 
Sbjct: 723 RKKEEKEKDPDSEAFKKQLVSWLKKNGLKLSTDTDSSG-----ATTSTNVLLNQMNSGLK 782

BLAST of Spg015060 vs. NCBI nr
Match: XP_022149859.1 (uncharacterized protein LOC111018186 [Momordica charantia] >XP_022149860.1 uncharacterized protein LOC111018186 [Momordica charantia])

HSP 1 Score: 227.6 bits (579), Expect = 3.3e-55
Identity = 108/217 (49.77%), Postives = 143/217 (65.90%), Query Frame = 0

Query: 132 ECRRINWDQTLVITRRCFHDEWSKIFDTLRNAFQKDLIINPFHPDKALLKCPDPDFARIV 191
           E RR+NW++T+VITRR FHD+WS+I   ++   +   IINPF  DKAL+KCP  D A ++
Sbjct: 40  EVRRLNWEETIVITRRDFHDDWSRILSYVKEQTESSYIINPFQADKALMKCPSKDLACLL 99

Query: 192 VHNKGWSVIGNFTLKFEYWNYKLHGRINVVPSYGSWLRFQNIPLQNWCLDTFKAIGDVYG 251
           + NKGW   G  T+K E WN  LHGR  + PSYG+W++ +NIPL  W L TFKAIG+  G
Sbjct: 100 LTNKGWVTFGPVTVKLEAWNPLLHGRAFLFPSYGNWVKIRNIPLHLWSLATFKAIGNALG 159

Query: 252 GFIECDDKCLSLVGCMEVVIRVRDNYCGFIPADFELMQADGSVSI-AQVVTFEDPQLLES 311
           GFI+ DD     + C +V I+V+ NYCGFIPA  E+   DG +   A+VV+FED + L  
Sbjct: 160 GFIDYDDNNSCFIECFDVAIKVKSNYCGFIPA--EISSMDGPLCFQAKVVSFEDSKWLAR 219

Query: 312 RRVYIHGGFSSEAARVSFGEDEGSDELCPVDKARMEN 348
           + V IHGGFSSEAAR SF +         +D+ R+EN
Sbjct: 220 KDVGIHGGFSSEAAR-SFHQGSAEQSANSIDRWRLEN 253

BLAST of Spg015060 vs. NCBI nr
Match: KAA0044449.1 (hypothetical protein E6C27_scaffold46G001820 [Cucumis melo var. makuwa])

HSP 1 Score: 222.6 bits (566), Expect = 1.1e-53
Identity = 202/782 (25.83%), Postives = 320/782 (40.92%), Query Frame = 0

Query: 7   RKCVAERKLFEIDYIKERNKRAVKISEKNHALRFEIILEVKHAIWIADVVDDLLITPISQ 66
           R C  E+K F +   K   +  + I+E      F I +      W+      LL TP + 
Sbjct: 9   RHCSVEKKEFVLSVDKRSRESNLLITEVGPYKSFSIAITPDSLEWLKMTFKALLNTPRTT 68

Query: 67  KFFRKTSCENGFIWFQKVSNKRGVFAEISKVTSSRRRDNLIIPQGEDSTGWKAFLAMV-- 126
           +FF +    +  +W Q + N+RG  AEI +V    R+  +++P+G D TGW  F  M+  
Sbjct: 69  RFFVEKRYVDFCLWVQTIHNRRGYIAEIYRVDDRGRKCCILVPEGLDKTGWALFNDMLTC 128

Query: 127 -----------NDFFN--------------------------------------VSECRR 186
                        ++N                                       S+C +
Sbjct: 129 KKTSDKKETPTRHYYNQDKGKEKIQQSYDSSTDSESPRKTYAEAVSSSSSSSSSESDCSK 188

Query: 187 INWDQTLVITRRCFHDEWSKIFDTLRN-AFQKDLIIN--PFHPDKALLKCPDPDFARIVV 246
                T  + RRCFHD+W+KI D LR+   +KD      PFH DKALL   D + A+++ 
Sbjct: 189 TK--STSSLKRRCFHDDWAKIIDRLRDQTDKKDSCFRYIPFHADKALLFIKDKELAKLLC 248

Query: 247 HNKGWSVIGNFTLKFEYWNYKLHGRINVVPSYGSWLRFQNIPLQNWCLDTFKAIGDVYGG 306
            N GW+ +G F +KFE W+   H    V+PSYG W RF+ IPL  W L+TF  IG+ YGG
Sbjct: 249 KNIGWTTVGPFYVKFEKWSKNAHADTKVIPSYGGWTRFRGIPLHIWNLNTFIQIGEAYGG 308

Query: 307 FIECDDKCLSLVGCMEVVIRVRDNYCGFIPADFELMQADGSVSIAQVVTFEDPQLLESRR 366
           FI+   + ++ +   E +I+V++NY GF+PA  ++   +G   I Q VT    + L  R 
Sbjct: 309 FIDAAPESVNKLELTEALIKVKENYTGFLPAFIQIHDEEGHDFIIQTVTHPKGKWLRERN 368

Query: 367 VYIHGGFSSEAARVSFGEDEGSDELCPVDKARMENRSDRFQIQAALNQAGDVSISYKRGF 426
             IHG F+  AA       E  +E  P  +         F+   A+    D+  S K+G 
Sbjct: 369 PSIHGSFTKTAA-------ENFNEFNPYAE------QYTFRRNLAVIAKPDLPESSKKGS 428

Query: 427 QSFEKDKAENMT--------------------SRKGVSEEKMRPRVGSSSTSEKEENN-- 486
           +    D+    T                    S    S EK++ +   S  +EK++    
Sbjct: 429 KQMNNDRKMGPTKTIKKFNKTVSFLNLNYEGDSDNSNSSEKIQKKDHRSEMTEKKKGKQI 488

Query: 487 -TNEPRKAVGPTREKKQVWREKDLSDKGVSFALKSEAWDQEEAIEDIAAMFANEEVECL- 546
              E  + + P   K++V      S K  +F   + +   +         +A  E +   
Sbjct: 489 CCGEYNQKISPINTKRKV---SFSSPKNETFLFSAHSAPTKTLKLGSPMSYAKMEAKSKR 548

Query: 547 -GQNRKDKMAIVVSDSQEPLIEANPINTEEDDRREPSSPCGSGQKSGSVESGSKLAIPIL 606
             Q+ K K+  V S S E               RE S    S QK       ++  + + 
Sbjct: 549 PTQSIKKKVYRVKSRSME---------------RETSQT--SRQKDKGKIDPNEFELVVD 608

Query: 607 KETIEILWQNGLCIRPIPKKIGGGASKGKRLRRCSMKILSWNVRGLGDADKRRLVKELVR 666
              I +L          P  I    S         +K    ++      D+ +  KE +R
Sbjct: 609 LGHISLLSDTDFSCPESPSYIPSPTSP---TESDIVKDSLASMMTCAHEDREKKKKENIR 668

Query: 667 SSGPYIVLIQESKLGFVHRHIVKQVWSSRFISWSSFNSEGSS----GGILVLWKEKEV-- 702
                    ++ ++ F  R +   +  +     + FNS+ +S      I +L     V  
Sbjct: 669 EE------TEDDEVSF-KRKLTDWLKENNLRLAADFNSQFNSVTNDRMISILNGPPNVGV 728

BLAST of Spg015060 vs. NCBI nr
Match: KAA0041398.1 (hypothetical protein E6C27_scaffold206G00440 [Cucumis melo var. makuwa])

HSP 1 Score: 214.5 bits (545), Expect = 2.9e-51
Identity = 146/508 (28.74%), Postives = 224/508 (44.09%), Query Frame = 0

Query: 7   RKCVAERKLFEIDYIKERNKRAVKISEKNHALRFEIILEVKHAIWIADVVDDLLITPISQ 66
           R C  E+K F +   K   +  + I+E      F I + +    W+      LL TP + 
Sbjct: 9   RHCSVEKKDFVLSVDKRSRESNLLITEVGPYKSFSIAITLDSLEWLKTTFKALLNTPRTT 68

Query: 67  KFFRKTSCENGFIWFQKVSNKRGVFAEISKVTSSRRRDNLIIPQGEDSTGW--------- 126
           +FF +    +  +W QK+ N+RG  AEI +V    R+  +++P+G D TGW         
Sbjct: 69  RFFVEKRYVDFCLWVQKIHNRRGYIAEIYRVDDRGRKCCILVPEGLDKTGWALFSDMLTC 128

Query: 127 --------------------------------------KAFLAMVNDFF----------N 186
                                                 K +  +V+ F           N
Sbjct: 129 KKTSDKKEISTRHYYNQDKGKEKIQKPYDSSTDSESPRKTYAEVVSSFSSSESDYSKAKN 188

Query: 187 VSECRR-----------------INWDQTLVITRRCFHDEWSKIFDTLRNAFQKD---LI 246
            S  +R                 I+W++T++++RRCFHD+W KI D LR    K      
Sbjct: 189 TSSLKRFLPAMKSEIRKEERKNDIDWEKTIILSRRCFHDDWDKIIDRLREQTDKKESCFC 248

Query: 247 INPFHPDKALLKCPDPDFARIVVHNKGWSVIGNFTLKFEYWNYKLHGRINVVPSYGSWLR 306
             PFH DKALL   D D A+++  N GW+ +G F +KFE W+  +H    V+PSYG W R
Sbjct: 249 YVPFHADKALLFIKDKDLAKLLCKNNGWTTVGPFYVKFEKWSKSVHADTKVIPSYGGWTR 308

Query: 307 FQNIPLQNWCLDTFKAIGDVYGGFIECDDKCLSLVGCMEVVIRVRDNYCGFIPADFELMQ 366
           F+ IPL  W L+TF  IG+  GGFI+   + ++ +   E +I+V++NY GF+PA  ++  
Sbjct: 309 FRGIPLHIWNLNTFIHIGEACGGFIDAAPESVNKIELTEALIKVKENYTGFLPAFIQIHD 368

Query: 367 ADGSVSIAQVVTFEDPQLLESRRVYIHGGFSSEAARVSFGEDEGSDELCPVDKARMENRS 426
            +G V I Q+VT  + + L  R   IHG F   AA       E  +E  P  +       
Sbjct: 369 EEGHVFIIQIVTHPEGRWLRERNPSIHGSFKKTAA-------ENFNEFNPYAE------Q 428

Query: 427 DRFQIQAALNQAGDVSISYKRGFQSFEKDKAENMTSRKGVSEEKMRPRVGSSSTSEKEEN 438
             F+   A+    D+S S K G +    D+ +  T     + +K    +GS  +  K E 
Sbjct: 429 FTFRRNLAVISKLDLSESSKNGSKQMNADRKKGPTK----TIKKFNKTLGSPMSYAKMEA 488

BLAST of Spg015060 vs. NCBI nr
Match: TYK08190.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 211.8 bits (538), Expect = 1.9e-50
Identity = 185/759 (24.37%), Postives = 321/759 (42.29%), Query Frame = 0

Query: 51  WIADVVDDLLITPISQKFFRKTSCENGFIWFQKVSN--KRGVFAEISKVTSSRRRDNLII 110
           WI +   DLL T  ++ FF +   E+  +W +K  N  K  + AEI ++ +  R+ ++++
Sbjct: 23  WIRNCFKDLLDTSTTKHFFAERRMEDNCMWVRKTKNKSKTSITAEIFRIDNKGRKCSILV 82

Query: 111 PQGEDSTGWKAFLAMV-----------------------NDFFNV--------------- 170
           P+G DS GWK+FLA++                       +D F+                
Sbjct: 83  PEGPDSFGWKSFLALITFRSSAPTKRIRSEIRKEPVSTFSDSFSSDSDSSRKSYAKVLSD 142

Query: 171 ----------------SECRRI-------------NWDQTLVITRRCFHDEWSKIFDTLR 230
                           S  RR              ++++T++ITRRCFHD+W++I  +LR
Sbjct: 143 SSEDDNKKRYKATSDDSSSRRSSSIGFKPFTLSGNSFEKTVIITRRCFHDDWNRIMFSLR 202

Query: 231 NAFQKDLIINPFHPDKALLKCPDPDFARIVVHNK---GWSVIGNFTLKFEYWNYKLHGRI 290
              +      PF  DKA+L   +PD A+++  NK   GWS +GN+ +KFE W+  LH   
Sbjct: 203 KQSEIAFSYKPFQADKAILFL-NPDHAKLLCSNKGANGWSTVGNYQVKFESWDSNLHSFH 262

Query: 291 NVVPSYGSWLRFQNIPLQNWCLDTFKAIGDVYGGFIECDDKCLSLVGCMEVVIRVRDNYC 350
           +V+PSYG WLRF+ IPL  W  +TF+ IG   GGF++   + + +   ++  I+VR NY 
Sbjct: 263 SVIPSYGGWLRFRGIPLHLWNYNTFQHIGSACGGFLDVAKETMQMDKLIDAKIKVRYNYI 322

Query: 351 GFIPADFELMQADGSVSIAQVVTFEDPQLLESRRVYIHGGFSSEAARVSFGEDEGSDELC 410
           GF+PA   +    G   I   V   + + L  R V +HG F ++AA      +  ++   
Sbjct: 323 GFVPASILITDNQGENFIVTTVQPAEARWLVERNVRVHGSFRTKAADEFDQHNHLAETYT 382

Query: 411 PVDKARMENRSDRFQIQAALNQAGDVSISYKRGFQSFEKDKAENMTSRKGVSEEKMRPRV 470
                 +     R     +++ +   SISY    +     ++E     + +S+ +     
Sbjct: 383 YNGFQAIPPEPTRTHGDYSIHNSDKHSISYHTQAKKNNSSESEYDPFDQQLSDRRKEKGK 442

Query: 471 GSSSTSEKEENNTNEPRKAVGPTREKKQVWREKDLSDKGVSFALKSEAWDQEEAIEDIAA 530
                ++++  + ++               R K +S++ VSF L         +  +I  
Sbjct: 443 AILIINDQDHGHYSK---------------RSKRISNRKVSF-LSPGGIQSNSSNTEINT 502

Query: 531 MFANEEVECLG----------QNRKDKMAIVVSDSQEPLIEANPINTEEDDRREPSSPCG 590
              + E+  +           Q  K K+   +    +   E + ++ +E          G
Sbjct: 503 KGKSLEISTINDQFEKRWSPRQKTKTKLTYRIKKDPQESTEDHKLSLKETGE-------G 562

Query: 591 SGQKSGSVESGSKLAIPILKETIEILWQNGLC------------------IRPIPKKIGG 650
           S Q + SV+ G    I  L+  I+    +GL                    + +   +  
Sbjct: 563 SKQMNLSVDMG---PISPLESMIQSENNHGLDTFNNQTPDGNSKSTDSAEAKNLTVSVKE 622

Query: 651 GASKGKRLRRCSMKILSWNVRGLGDADKRRLVKELVRSSGPYIVLIQESKLGFVHRHIVK 702
           GA + K   R + +  S + +   + +  R  KE +      ++ ++E++L    ++   
Sbjct: 623 GADQNKSASRSTAEGNSKDAKTGSELEIDRAFKEKL------VIWLKENELKLSPKYTND 682

BLAST of Spg015060 vs. ExPASy Swiss-Prot
Match: Q9SKZ1 (Transcription factor Pur-alpha 1 OS=Arabidopsis thaliana OX=3702 GN=PURA1 PE=1 SV=2)

HSP 1 Score: 59.3 bits (142), Expect = 2.0e-07
Identity = 35/121 (28.93%), Postives = 58/121 (47.93%), Query Frame = 0

Query: 12  ERKLFEIDYIKERNKRAVKISEKNHALRFEIILEVKHAIWIADVVDDLLITPISQKFFRK 71
           E KLF  D  +    R +KISEK  A R  II+      W  D+ +  + +   + F ++
Sbjct: 39  EHKLFYFDLKENPRGRYLKISEKTSATRSTIIVPSSGISWFLDLFNYYVNSEEHELFSKE 98

Query: 72  TSCENGFIWFQKVSNKRGVFAEISKVTSSRRRDNLIIPQGED-STGWKAFLAMVNDFFNV 131
              ++   +F    N+RG F ++S+ + SR R  +I+P G     GW AF  ++ +    
Sbjct: 99  LQLDSKVFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSSPDEGWAAFRNILAEIHEA 158

BLAST of Spg015060 vs. ExPASy TrEMBL
Match: A0A5D3BLV7 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G005290 PE=4 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 6.4e-57
Identity = 198/819 (24.18%), Postives = 339/819 (41.39%), Query Frame = 0

Query: 7   RKCVAERKLFEIDYIKERNKRAVKISEKNHALRFEIILEVKHAIWIADVVDDLLITPISQ 66
           R C  ERK F +   K        ++E      F I +  +   WI   +  L+ TP + 
Sbjct: 63  RSCKVERKEFVLHLDKYSKHTHYWLTETGAHKAFSIEVSPRDLDWIRCTLKSLIATPNTN 122

Query: 67  KFFRKTSCENGFIWFQKVSNKRGVFAEISKVTSSRRRDNLIIPQGEDSTGWKAFLAMVN- 126
           +FF +T      IW +K  N +G  AEI +V    R+  +++P+G D +GW +FL+M+  
Sbjct: 123 RFFLETRDSEQRIWIRKTRNSKGCTAEIFRVDQKNRKSCILVPEGPDKSGWVSFLSMITP 182

Query: 127 --------------------------DFFNVSECRRIN---------------------- 186
                                     D+   S  + +                       
Sbjct: 183 KVEVKAKTRPTFLPRTSPDCRLSPPIDYHKRSYAKAVTEGRPFATSDSSDSYDSSDSSHS 242

Query: 187 -------------WDQTLVITRRCFHDEWSKIFDTLRNAFQKDLIINPFHPDKALLKCPD 246
                         + T+VI RR FHD+W KI   LR   ++    N FH +KAL+    
Sbjct: 243 SSNSFCDSPSSDLLENTVVIVRRFFHDDWHKILQNLRKQTEESFTYNAFHAEKALVHFSS 302

Query: 247 PDFARIVVHNKGWSVIGNFTLKFEYWNYKLHGRINVVPSYGSWLRFQNIPLQNWCLDTFK 306
              A ++  NKGWS +G ++++FE W+   H    ++PSYG W  F+ IPL  W + TF+
Sbjct: 303 NIPANLLCQNKGWSTVGKYSVRFEKWSPVYHATPKLIPSYGGWTTFRGIPLHLWNMMTFQ 362

Query: 307 AIGDVYGGFIECDDKCLSLVGCMEVVIRVRDNYCGFIPADFELMQADGSVSIAQVVTFED 366
            IG    G I+  ++  S    +E  I+VR NY GF+PA+  +   +G+    QVVT  +
Sbjct: 363 QIGKACEGLIKVAEETRSAKNLIEARIKVRYNYSGFLPANVRIFDNEGNKFFVQVVTHPE 422

Query: 367 PQLLESRRVYIHGGFSSEAARVSFGE---------DEGSDELCP-----VDKARMENRSD 426
            + L  R V +HG F  +AA  SF +          EGS+ + P         R  +  D
Sbjct: 423 GKWLIERNVRLHGTFKRQAA-ASFDDFNPESEQFFFEGSEAISPDFLSTSSDGRKSSTPD 482

Query: 427 R-------------------FQIQAALNQAGDVSISYKR-----------GFQSFEKDKA 486
           +                   F  +  +N +   + + K            G     K K 
Sbjct: 483 QPSALKSVIIKPDRNATLPSFLNEELVNDSNLHATANKSKLEILSGISNDGVLDKGKQKV 542

Query: 487 E-NMTSRKGVSEEKMRPRVGSSSTSEKEE--NNTNEPRK---AVGPTREKKQVWREKDLS 546
           +  +     ++ +K + +V  +S S K    N  + P     ++    +K++V RE+ + 
Sbjct: 543 DIQLQPNSALNLDKSKRKVSFNSPSNKTNIFNPDSAPANHSPSLNSPEKKQKVSRERSIK 602

Query: 547 DKGVSFALKSEAWDQEEAIEDIAAMFANEEVECLGQNR---KDKMAIVVSDSQEPLIEAN 606
            K  S    S+A +Q + +      F  + ++ +  +R   K  +++ V     P ++ N
Sbjct: 603 KKSSSTQPNSKA-NQNKGV------FITQPIQIVAHDRDAAKKGLSLTVDLGDLPALDPN 662

Query: 607 PINTEEDDRREPSSPCGSGQKSGSVESGSKLAIPILKETIEILWQNGLCIRPIPKKIGGG 666
              + ED     ++       +  V    ++ +P+ + +      N    + + K+    
Sbjct: 663 --KSLEDHHNSDNAEVVDITNTEVVPETPEMKMPVNENSNSSSEANYRKPKHVHKRKYYY 722

Query: 667 ASKGKRLR-----RCSMKILSW----NVRGLGDADKRRLVKELVRSSGPYIVLIQESKLG 702
             K ++ +         +++SW     ++   D D          +S   ++    S L 
Sbjct: 723 RKKEEKEKDPDSEAFKKQLVSWLKKNGLKLSTDTDSSG-----ATTSTNVLLNQMNSGLK 782

BLAST of Spg015060 vs. ExPASy TrEMBL
Match: A0A6J1D6X4 (uncharacterized protein LOC111018186 OS=Momordica charantia OX=3673 GN=LOC111018186 PE=4 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 1.6e-55
Identity = 108/217 (49.77%), Postives = 143/217 (65.90%), Query Frame = 0

Query: 132 ECRRINWDQTLVITRRCFHDEWSKIFDTLRNAFQKDLIINPFHPDKALLKCPDPDFARIV 191
           E RR+NW++T+VITRR FHD+WS+I   ++   +   IINPF  DKAL+KCP  D A ++
Sbjct: 40  EVRRLNWEETIVITRRDFHDDWSRILSYVKEQTESSYIINPFQADKALMKCPSKDLACLL 99

Query: 192 VHNKGWSVIGNFTLKFEYWNYKLHGRINVVPSYGSWLRFQNIPLQNWCLDTFKAIGDVYG 251
           + NKGW   G  T+K E WN  LHGR  + PSYG+W++ +NIPL  W L TFKAIG+  G
Sbjct: 100 LTNKGWVTFGPVTVKLEAWNPLLHGRAFLFPSYGNWVKIRNIPLHLWSLATFKAIGNALG 159

Query: 252 GFIECDDKCLSLVGCMEVVIRVRDNYCGFIPADFELMQADGSVSI-AQVVTFEDPQLLES 311
           GFI+ DD     + C +V I+V+ NYCGFIPA  E+   DG +   A+VV+FED + L  
Sbjct: 160 GFIDYDDNNSCFIECFDVAIKVKSNYCGFIPA--EISSMDGPLCFQAKVVSFEDSKWLAR 219

Query: 312 RRVYIHGGFSSEAARVSFGEDEGSDELCPVDKARMEN 348
           + V IHGGFSSEAAR SF +         +D+ R+EN
Sbjct: 220 KDVGIHGGFSSEAAR-SFHQGSAEQSANSIDRWRLEN 253

BLAST of Spg015060 vs. ExPASy TrEMBL
Match: A0A5A7TTA1 (DUF4283 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G001820 PE=4 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 5.1e-54
Identity = 202/782 (25.83%), Postives = 320/782 (40.92%), Query Frame = 0

Query: 7   RKCVAERKLFEIDYIKERNKRAVKISEKNHALRFEIILEVKHAIWIADVVDDLLITPISQ 66
           R C  E+K F +   K   +  + I+E      F I +      W+      LL TP + 
Sbjct: 9   RHCSVEKKEFVLSVDKRSRESNLLITEVGPYKSFSIAITPDSLEWLKMTFKALLNTPRTT 68

Query: 67  KFFRKTSCENGFIWFQKVSNKRGVFAEISKVTSSRRRDNLIIPQGEDSTGWKAFLAMV-- 126
           +FF +    +  +W Q + N+RG  AEI +V    R+  +++P+G D TGW  F  M+  
Sbjct: 69  RFFVEKRYVDFCLWVQTIHNRRGYIAEIYRVDDRGRKCCILVPEGLDKTGWALFNDMLTC 128

Query: 127 -----------NDFFN--------------------------------------VSECRR 186
                        ++N                                       S+C +
Sbjct: 129 KKTSDKKETPTRHYYNQDKGKEKIQQSYDSSTDSESPRKTYAEAVSSSSSSSSSESDCSK 188

Query: 187 INWDQTLVITRRCFHDEWSKIFDTLRN-AFQKDLIIN--PFHPDKALLKCPDPDFARIVV 246
                T  + RRCFHD+W+KI D LR+   +KD      PFH DKALL   D + A+++ 
Sbjct: 189 TK--STSSLKRRCFHDDWAKIIDRLRDQTDKKDSCFRYIPFHADKALLFIKDKELAKLLC 248

Query: 247 HNKGWSVIGNFTLKFEYWNYKLHGRINVVPSYGSWLRFQNIPLQNWCLDTFKAIGDVYGG 306
            N GW+ +G F +KFE W+   H    V+PSYG W RF+ IPL  W L+TF  IG+ YGG
Sbjct: 249 KNIGWTTVGPFYVKFEKWSKNAHADTKVIPSYGGWTRFRGIPLHIWNLNTFIQIGEAYGG 308

Query: 307 FIECDDKCLSLVGCMEVVIRVRDNYCGFIPADFELMQADGSVSIAQVVTFEDPQLLESRR 366
           FI+   + ++ +   E +I+V++NY GF+PA  ++   +G   I Q VT    + L  R 
Sbjct: 309 FIDAAPESVNKLELTEALIKVKENYTGFLPAFIQIHDEEGHDFIIQTVTHPKGKWLRERN 368

Query: 367 VYIHGGFSSEAARVSFGEDEGSDELCPVDKARMENRSDRFQIQAALNQAGDVSISYKRGF 426
             IHG F+  AA       E  +E  P  +         F+   A+    D+  S K+G 
Sbjct: 369 PSIHGSFTKTAA-------ENFNEFNPYAE------QYTFRRNLAVIAKPDLPESSKKGS 428

Query: 427 QSFEKDKAENMT--------------------SRKGVSEEKMRPRVGSSSTSEKEENN-- 486
           +    D+    T                    S    S EK++ +   S  +EK++    
Sbjct: 429 KQMNNDRKMGPTKTIKKFNKTVSFLNLNYEGDSDNSNSSEKIQKKDHRSEMTEKKKGKQI 488

Query: 487 -TNEPRKAVGPTREKKQVWREKDLSDKGVSFALKSEAWDQEEAIEDIAAMFANEEVECL- 546
              E  + + P   K++V      S K  +F   + +   +         +A  E +   
Sbjct: 489 CCGEYNQKISPINTKRKV---SFSSPKNETFLFSAHSAPTKTLKLGSPMSYAKMEAKSKR 548

Query: 547 -GQNRKDKMAIVVSDSQEPLIEANPINTEEDDRREPSSPCGSGQKSGSVESGSKLAIPIL 606
             Q+ K K+  V S S E               RE S    S QK       ++  + + 
Sbjct: 549 PTQSIKKKVYRVKSRSME---------------RETSQT--SRQKDKGKIDPNEFELVVD 608

Query: 607 KETIEILWQNGLCIRPIPKKIGGGASKGKRLRRCSMKILSWNVRGLGDADKRRLVKELVR 666
              I +L          P  I    S         +K    ++      D+ +  KE +R
Sbjct: 609 LGHISLLSDTDFSCPESPSYIPSPTSP---TESDIVKDSLASMMTCAHEDREKKKKENIR 668

Query: 667 SSGPYIVLIQESKLGFVHRHIVKQVWSSRFISWSSFNSEGSS----GGILVLWKEKEV-- 702
                    ++ ++ F  R +   +  +     + FNS+ +S      I +L     V  
Sbjct: 669 EE------TEDDEVSF-KRKLTDWLKENNLRLAADFNSQFNSVTNDRMISILNGPPNVGV 728

BLAST of Spg015060 vs. ExPASy TrEMBL
Match: A0A5A7TEP0 (DUF4283 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold206G00440 PE=4 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 1.4e-51
Identity = 146/508 (28.74%), Postives = 224/508 (44.09%), Query Frame = 0

Query: 7   RKCVAERKLFEIDYIKERNKRAVKISEKNHALRFEIILEVKHAIWIADVVDDLLITPISQ 66
           R C  E+K F +   K   +  + I+E      F I + +    W+      LL TP + 
Sbjct: 9   RHCSVEKKDFVLSVDKRSRESNLLITEVGPYKSFSIAITLDSLEWLKTTFKALLNTPRTT 68

Query: 67  KFFRKTSCENGFIWFQKVSNKRGVFAEISKVTSSRRRDNLIIPQGEDSTGW--------- 126
           +FF +    +  +W QK+ N+RG  AEI +V    R+  +++P+G D TGW         
Sbjct: 69  RFFVEKRYVDFCLWVQKIHNRRGYIAEIYRVDDRGRKCCILVPEGLDKTGWALFSDMLTC 128

Query: 127 --------------------------------------KAFLAMVNDFF----------N 186
                                                 K +  +V+ F           N
Sbjct: 129 KKTSDKKEISTRHYYNQDKGKEKIQKPYDSSTDSESPRKTYAEVVSSFSSSESDYSKAKN 188

Query: 187 VSECRR-----------------INWDQTLVITRRCFHDEWSKIFDTLRNAFQKD---LI 246
            S  +R                 I+W++T++++RRCFHD+W KI D LR    K      
Sbjct: 189 TSSLKRFLPAMKSEIRKEERKNDIDWEKTIILSRRCFHDDWDKIIDRLREQTDKKESCFC 248

Query: 247 INPFHPDKALLKCPDPDFARIVVHNKGWSVIGNFTLKFEYWNYKLHGRINVVPSYGSWLR 306
             PFH DKALL   D D A+++  N GW+ +G F +KFE W+  +H    V+PSYG W R
Sbjct: 249 YVPFHADKALLFIKDKDLAKLLCKNNGWTTVGPFYVKFEKWSKSVHADTKVIPSYGGWTR 308

Query: 307 FQNIPLQNWCLDTFKAIGDVYGGFIECDDKCLSLVGCMEVVIRVRDNYCGFIPADFELMQ 366
           F+ IPL  W L+TF  IG+  GGFI+   + ++ +   E +I+V++NY GF+PA  ++  
Sbjct: 309 FRGIPLHIWNLNTFIHIGEACGGFIDAAPESVNKIELTEALIKVKENYTGFLPAFIQIHD 368

Query: 367 ADGSVSIAQVVTFEDPQLLESRRVYIHGGFSSEAARVSFGEDEGSDELCPVDKARMENRS 426
            +G V I Q+VT  + + L  R   IHG F   AA       E  +E  P  +       
Sbjct: 369 EEGHVFIIQIVTHPEGRWLRERNPSIHGSFKKTAA-------ENFNEFNPYAE------Q 428

Query: 427 DRFQIQAALNQAGDVSISYKRGFQSFEKDKAENMTSRKGVSEEKMRPRVGSSSTSEKEEN 438
             F+   A+    D+S S K G +    D+ +  T     + +K    +GS  +  K E 
Sbjct: 429 FTFRRNLAVISKLDLSESSKNGSKQMNADRKKGPTK----TIKKFNKTLGSPMSYAKMEA 488

BLAST of Spg015060 vs. ExPASy TrEMBL
Match: A0A5A7US62 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold280G003960 PE=4 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 9.0e-51
Identity = 185/759 (24.37%), Postives = 321/759 (42.29%), Query Frame = 0

Query: 51  WIADVVDDLLITPISQKFFRKTSCENGFIWFQKVSN--KRGVFAEISKVTSSRRRDNLII 110
           WI +   DLL T  ++ FF +   E+  +W +K  N  K  + AEI ++ +  R+ ++++
Sbjct: 23  WIRNCFKDLLDTSTTKHFFAERRMEDNCMWVRKTKNKSKTSITAEIFRIDNKGRKCSILV 82

Query: 111 PQGEDSTGWKAFLAMV-----------------------NDFFNV--------------- 170
           P+G DS GWK+FLA++                       +D F+                
Sbjct: 83  PEGPDSFGWKSFLALITFRSSAPTKRIRSEIRKEPVSTFSDSFSSDSDSSRKSYAKVLSD 142

Query: 171 ----------------SECRRI-------------NWDQTLVITRRCFHDEWSKIFDTLR 230
                           S  RR              ++++T++ITRRCFHD+W++I  +LR
Sbjct: 143 SSEDDNKKRYKATSDDSSSRRSSSIGFKPFTLSGNSFEKTVIITRRCFHDDWNRIMFSLR 202

Query: 231 NAFQKDLIINPFHPDKALLKCPDPDFARIVVHNK---GWSVIGNFTLKFEYWNYKLHGRI 290
              +      PF  DKA+L   +PD A+++  NK   GWS +GN+ +KFE W+  LH   
Sbjct: 203 KQSEIAFSYKPFQADKAILFL-NPDHAKLLCSNKGANGWSTVGNYQVKFESWDSNLHSFH 262

Query: 291 NVVPSYGSWLRFQNIPLQNWCLDTFKAIGDVYGGFIECDDKCLSLVGCMEVVIRVRDNYC 350
           +V+PSYG WLRF+ IPL  W  +TF+ IG   GGF++   + + +   ++  I+VR NY 
Sbjct: 263 SVIPSYGGWLRFRGIPLHLWNYNTFQHIGSACGGFLDVAKETMQMDKLIDAKIKVRYNYI 322

Query: 351 GFIPADFELMQADGSVSIAQVVTFEDPQLLESRRVYIHGGFSSEAARVSFGEDEGSDELC 410
           GF+PA   +    G   I   V   + + L  R V +HG F ++AA      +  ++   
Sbjct: 323 GFVPASILITDNQGENFIVTTVQPAEARWLVERNVRVHGSFRTKAADEFDQHNHLAETYT 382

Query: 411 PVDKARMENRSDRFQIQAALNQAGDVSISYKRGFQSFEKDKAENMTSRKGVSEEKMRPRV 470
                 +     R     +++ +   SISY    +     ++E     + +S+ +     
Sbjct: 383 YNGFQAIPPEPTRTHGDYSIHNSDKHSISYHTQAKKNNSSESEYDPFDQQLSDRRKEKGK 442

Query: 471 GSSSTSEKEENNTNEPRKAVGPTREKKQVWREKDLSDKGVSFALKSEAWDQEEAIEDIAA 530
                ++++  + ++               R K +S++ VSF L         +  +I  
Sbjct: 443 AILIINDQDHGHYSK---------------RSKRISNRKVSF-LSPGGIQSNSSNTEINT 502

Query: 531 MFANEEVECLG----------QNRKDKMAIVVSDSQEPLIEANPINTEEDDRREPSSPCG 590
              + E+  +           Q  K K+   +    +   E + ++ +E          G
Sbjct: 503 KGKSLEISTINDQFEKRWSPRQKTKTKLTYRIKKDPQESTEDHKLSLKETGE-------G 562

Query: 591 SGQKSGSVESGSKLAIPILKETIEILWQNGLC------------------IRPIPKKIGG 650
           S Q + SV+ G    I  L+  I+    +GL                    + +   +  
Sbjct: 563 SKQMNLSVDMG---PISPLESMIQSENNHGLDTFNNQTPDGNSKSTDSAEAKNLTVSVKE 622

Query: 651 GASKGKRLRRCSMKILSWNVRGLGDADKRRLVKELVRSSGPYIVLIQESKLGFVHRHIVK 702
           GA + K   R + +  S + +   + +  R  KE +      ++ ++E++L    ++   
Sbjct: 623 GADQNKSASRSTAEGNSKDAKTGSELEIDRAFKEKL------VIWLKENELKLSPKYTND 682

BLAST of Spg015060 vs. TAIR 10
Match: AT2G32080.1 (purin-rich alpha 1 )

HSP 1 Score: 59.3 bits (142), Expect = 1.4e-08
Identity = 35/121 (28.93%), Postives = 58/121 (47.93%), Query Frame = 0

Query: 12  ERKLFEIDYIKERNKRAVKISEKNHALRFEIILEVKHAIWIADVVDDLLITPISQKFFRK 71
           E KLF  D  +    R +KISEK  A R  II+      W  D+ +  + +   + F ++
Sbjct: 39  EHKLFYFDLKENPRGRYLKISEKTSATRSTIIVPSSGISWFLDLFNYYVNSEEHELFSKE 98

Query: 72  TSCENGFIWFQKVSNKRGVFAEISKVTSSRRRDNLIIPQGED-STGWKAFLAMVNDFFNV 131
              ++   +F    N+RG F ++S+ + SR R  +I+P G     GW AF  ++ +    
Sbjct: 99  LQLDSKVFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSSPDEGWAAFRNILAEIHEA 158

BLAST of Spg015060 vs. TAIR 10
Match: AT2G32080.2 (purin-rich alpha 1 )

HSP 1 Score: 59.3 bits (142), Expect = 1.4e-08
Identity = 35/121 (28.93%), Postives = 58/121 (47.93%), Query Frame = 0

Query: 12  ERKLFEIDYIKERNKRAVKISEKNHALRFEIILEVKHAIWIADVVDDLLITPISQKFFRK 71
           E KLF  D  +    R +KISEK  A R  II+      W  D+ +  + +   + F ++
Sbjct: 39  EHKLFYFDLKENPRGRYLKISEKTSATRSTIIVPSSGISWFLDLFNYYVNSEEHELFSKE 98

Query: 72  TSCENGFIWFQKVSNKRGVFAEISKVTSSRRRDNLIIPQGED-STGWKAFLAMVNDFFNV 131
              ++   +F    N+RG F ++S+ + SR R  +I+P G     GW AF  ++ +    
Sbjct: 99  LQLDSKVFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSSPDEGWAAFRNILAEIHEA 158

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
TYJ99315.11.3e-5624.18LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa][more]
XP_022149859.13.3e-5549.77uncharacterized protein LOC111018186 [Momordica charantia] >XP_022149860.1 uncha... [more]
KAA0044449.11.1e-5325.83hypothetical protein E6C27_scaffold46G001820 [Cucumis melo var. makuwa][more]
KAA0041398.12.9e-5128.74hypothetical protein E6C27_scaffold206G00440 [Cucumis melo var. makuwa][more]
TYK08190.11.9e-5024.37LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q9SKZ12.0e-0728.93Transcription factor Pur-alpha 1 OS=Arabidopsis thaliana OX=3702 GN=PURA1 PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A5D3BLV76.4e-5724.18LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119... [more]
A0A6J1D6X41.6e-5549.77uncharacterized protein LOC111018186 OS=Momordica charantia OX=3673 GN=LOC111018... [more]
A0A5A7TTA15.1e-5425.83DUF4283 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2... [more]
A0A5A7TEP01.4e-5128.74DUF4283 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2... [more]
A0A5A7US629.0e-5124.37LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119... [more]
Match NameE-valueIdentityDescription
AT2G32080.11.4e-0828.93purin-rich alpha 1 [more]
AT2G32080.21.4e-0828.93purin-rich alpha 1 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036691Endonuclease/exonuclease/phosphatase superfamilyGENE3D3.60.10.10Endonuclease/exonuclease/phosphatasecoord: 559..701
e-value: 1.6E-15
score: 59.5
IPR036691Endonuclease/exonuclease/phosphatase superfamilySUPERFAMILY56219DNase I-likecoord: 561..699
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 377..430
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 483..517
NoneNo IPR availablePANTHERPTHR22748:SF11DNA-(APURINIC OR APYRIMIDINIC SITE) LYASE CHLOROPLASTICcoord: 399..701
IPR004808AP endonuclease 1PANTHERPTHR22748AP ENDONUCLEASEcoord: 399..701

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg015060.1Spg015060.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006281 DNA repair
molecular_function GO:0004518 nuclease activity