Spg014981 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg014981
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionDDB1- and CUL4-associated factor 8
Locationscaffold3: 31287890 .. 31293050 (+)
RNA-Seq ExpressionSpg014981
SyntenySpg014981
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAAAAAAAAAGAAAAGAAAAAAGAAAGAAAAAGGAAAGACCAAAATCTGAACAAGAGACGTGGCTTGAATCATGATGTTGTGAAGGAGAAAGAGCGGTCAGATAAGCTTAGAAGAAAGAGTGTTTGCATTTGGATGTGTGTTTTAACGTGGAGTTCTCGAATAGGCCGATCAACATAATCCTGTGAGCGACTCCGATGCTCTTCTGCTTCTACTTAAAGCGGTGAACGGCGAAATCCTCTGTTCGTTTGCCATGCCGATGCCCTTCTTCCATTACTTTAACGCCCTCGATCGCCTCTTGTTCGTTTCCGTAATCCGATCATACTGAAATTCGTTCGGTTCTTTTAAGCGTATTGGGATTCAATAATATGAACAAGAGGGCCAGAATTGGTCATTCCAATAAGGCTGTGGTCAATGTCTGGAAGCGGGAGGTCGGCGAGCTCTCCTCTAGAGATTTCGCCCACCGTCTTGGTGCCTCGGAGGTGTTGCTTATCCTTCTCTCATTCTCTATCTTGATTTCTGCTTTACCGGTTATTTTTTGTATGCCCAAATAATCTTGTCAGGTTGTAGAGTTGTTCTGATTGTCAAATGTTGTCGTTTGTCGTTTGATCAGAACTGGATAAACCAACCGGAGTGAGTCTAGTTGTGTTGCATTATAAAAGGGTTTTATTTTGATTGAGATTATACAGGTTTTGGTGGATAGTTTACTAGCTCAAATAGTTGGAGTATTCGTGTTGATATTTATACTGTTTCAAAGCTGTGTTAGTATTCGTGTTGTTTCCGCAGCTGAGAATCTATGGACCCTTTTGATTGTTAAACCTGCAGGATTAATCGTTGTTGTTATTACTGTAATTTCAGGATCTCGTGCTCCGACTTGATCTTTTCAGGAAATTGGAGAGGCACAAAGGTTGTGTGAATACTGTGAGCTTTAGTGCTGCTGGTGACATTCTAGTGTCAGGCTCTGATGATAGGCGGGTGATCCTCTGGAATTGGGAAACCGGGCATGCCAAACTCTGTTTTCAGTCTGGTCATAATAATAATGTTTTCCAAGCAAAAATCATGCCTTACACGGATGACAATAGCATTGTGACTTGTGCTGCGGATGGGCAGGTATATTATCTAGTTGAAGAGCTGATTTTTTCTAGACCGTACAACATGGAATTAGACCCTTGTGGTCTAACATGGTTCTTGTTTTAGTACTCATGTCAAGAACTCGTACTTTCTCTCTCAGTATAGGTATGCTGTACATTTAGCTAAAATACTATAGTTTCTCTCTAGCCTAATGGATCATAATGGTGCTTCGATTGCACCTTTCCACAATTATTTAGCTTTCCTTTTGAGCCACCTTCTGAGGTGATTCTAGGAGCCAATTTGCAGTGTCATTAGGGAAACAACAGTTAGTCCAAAGGTTTTCAAAAGGGATAACCGCCTAGCCACAAACTCACACTGAACGAATTGGAGATTCGTAGTCTCTTCATCCTTTTTTACATTTTGGGGAACATCTGAGGCTGAGGCTGGTTATGGAGGAAGCAACTTCTTCTTTGAATTCTATCTTCGATTGTTTTGATGTCTGTTTTACACATCTTTTAAATCCACTTTCAAATTTTCACGCTAAAACTGTCTAATATTGACCAACCTTTATTGAGGCCTTTGACGAGACACTGGCTTTGTCAGGGTGATAAAGATTGATGACAGAAGTTCCTTTGCAGCTGGCTTTGTATATTATCAAGAATTCTTCCCTTGCTATCGTGGAAGCAACATCTTGTGATGACTATCACTAGAGGCTCTCCTTTCCTAGAATATTTGGCGCTTGTTCATTCAAGCGGGAGATTGTCAGTCTTTCACTACATCTGGCTGCTTATGCCAAATATCTCTCCTTCCCGATAAAGTTTTCCTTCACCGGAGCTGTTTATTCTACCCCTCAAAGAATCTTCTGCTCAGCAAAAGAAACTGAATAATTTTCTCCCTCTGGAATCATTAAACTCAATCTCTTTCCACTACTTCGGATGTGAGAAGTCTTTATGAAATCCCAATTGTTGAAATTTTCTTGATCCAAATGAAGCGATTTCCACACTCAGTCTTCCAAAACAATCCTACATAAAGTGGTGTTAAAAGGAGGTTCTCCACAATCAAATTGACTTTCTTTGTTCCACTTGAAAATACTTGCCTCTCCATTATCGGTCAAACTCGACCTATAACCATTTCTTCTTGATGCCTTGTGCTTATGGCTTTAGGTGGTTTTATTCTTGATGCTCTTCAACAACAACAGCAACTGTGATGTCTACTGACATTGATTTATTAATTCATGTTCTGAAAAAGAATTTCTCTTTTTCTCAATTTAAGTAAAACTTCCTGTTTATTTTCTGTCAGCGGAATATTTACTTTGTTTTCTCTTATCAACAATAGTTTTGTTGTTTACTAACATTGATTTATTAAATTATAATTTAAAAAAGAATTCTGTCCTTCTCAATTTAAGTTAAACAGCCTGTTTATTTTCTGTGAGCAGAATATTTACTTTGTTTTCTCCCGCTGTAAAACCTGTCTATTTGTGCTCTGTATTAAAACATTCATTTCTTGAAGAGTATTATTTGCTGAAATTCAGGTGAGACATGCTCAGATTCTGGATGGTGGGGATGTGGAAACTGCATTACTTGGCAGTCATCAAGGGCGTGCTCATAAGTTGGCCATTGAACCTGGGAGCCCTTATATCTTTTATACTTGTGGTGAAGATGGATTGGTTCAACATGTAAGTTTGGTTGTTCATTCTGTTGTTATATTATGTTCCGCCAACATTCCTAGTACTATTACAACTCAATAAGTTAAATACTTATTCACAGCCAAAGAACTCTACACGTTTTAAAGATTTATTACTGAACCTAAAGAATGAAGTGAGATGTTTTCTAAAGACCATTTATCTTTAATATCTTTGAATTGCAATGTGATACTGCCCACTTTACTTTTTTAACAGTTTGATCTAAGAACTGGTGTTGCCATTGAGCTGTTCACTTGCCAGTCAGTTGACAAGAGGGCAGGTTACATGTCATCGATTCAGCTAAATGCAATTGCTATTGATCCCAGAAATCCAAATCTCTTTGTCGTTGCTGGTTCAGATGAGTATGCACGGTTGTATGATATTCGCAAATATAAGGGGGAAGGATCAACTGATTTTGGTCAACCTGCAGACTGCTATTGCCCCCCTCACTTGATTGGTGATGAACAAGTGGGAATAACAGGTTTAGCCTTCTCAGAGCTGAGTGAACTCCTTGTTTCATACAATGATGAATCTATTTATCTCTTTAATAGGGATATGGGTTTGGGCCGAAGCCCATATCCATTGTCTCCAGGTACAAGTGATATGGGAGCTGAAAAAGTTAAGGATGGTAGTCCACAAGTTTATAAAGGACATAGAAACTGTGAGACAGTAAAAGGTGTCAATTTTTTTGGACCTAAATGTGAGTATGTCATCAGTGGATCTGATTGTGGCAGAATATTTATATGGAGGAAAAAGGGTGGAGTGCTAATCCGAGTCATGGAAGCAGACGAGGATGTGGTGAACTGCATTGAGCCCCATCCTCACATGACCATGCTTGCTAGTAGTGGAATTGAAAGTGACGTCAAGTTATGGACTCCAAAGGCCCTTGAAAGGGCTACTTTGCCTGAGAAAATTGAACAGGTAGGAATGTCATCTCCATTCTTCTGTTTAATGCCAATAGTGATGCTTTACTATAGCCTATTGAATGGCATTTGACTCTTTGAATTATTGAGGTTTCTTTTGTATAAATTGAATAAAACATCCGATGTCCATTCCAGCCATAAGGATAATAGCACCGGCCATTTCATATTCTTGACTCTCCTATATTTATTCAAACATGATCATATTTCCTCTATTTTTTATCAGTCTGTGTTACTGTCTTCTGCTTGTATGGATGGATGAGTTTGTGTCGCTCTTTTCCCCCTTGTTTTTCTGCATGTAGACTTGTAGAGTCATAAGAATCAGAATGAACGTGTCCTATAAATTTGTGTCATTCTATATCGCCATTTTTTTTTTGAGAATATCTATATCACCAATTGAAGTTATCTTGTATGATGACCTTAATAACATTTTGGGGTGTTTGTTTTTAACAAATCATTTGTGTTTTTTAAAGACTATGTTCTTTGGAACAACAAGGTGGGATGTTGAGTATGTTTCTAGTACATTTCTCGACCAATTAATGCCATAAGAACATGATATGGGCATGTGGTAGTCTTGTTCAATGAAAAGAAAGAAAGAAACCACGAGGCAATTGAGATAGACTCATAAGTAAGCTATAGTTATCCAGATTCCATAGAGAATCTTTGGTAACTGATGTCAACATTTTATATGTGAACAGGATTATGGTTAATACACATATTCATATACATTCTGTAGATGACATGAGCAGAGGTTTATTTCTCTGGTGTTTATAAATGACCTGGTTCAATTCCTTCATTTGGTGAAGAACATAGGTTTTGTGATGATTGGTATTGTTTTCAATGATGATTACAAAGATGATGAAGACAATGAAGGCAACGATGATAAGGGGAAAGTATGACAGAGATGTTCATAAATTTCATTCTTCTGATCTAATGATTTTGAACTGTGCATTTTGCAGAAACTGAAGCCTAGGGGATGGATGCACCGGTTAGCTTTGCCGGAGGAACTGATGATGCAACTGTTTTCACTGCGAAGACAGAATTCCAGCCCTGAACACCGAAGAGAAAACTCCGCTGTAGGTAGGGAAATTTTAGAGTTGATAATGACATTCAATAACAACACCGAGAGCTCTTCAGAAGACAACGGTGGTGATACTGCTGAAGATGATTGATATAATATAATTGAGAGAATGTTTATGCTGACCTCTTGGCTATAAATTTTAATAATTTGATAGCTAATTGTGCATAAAATAGAAACCGAGGCATGCATTATGAATCTAATCTTTCAAATTTTCATATGAATTAATAGCTATTAGCCCTCCTAGCTATATATGAATTCCTCCTGTGTTGACTATAAATTTAGTTGGTGCGTTCTTCGGGTCGTTTTGTTTGTTTCATTTTTCATGAGGAGTTCTGATGTAAATGAGCTGCCATCGTTGTGAATGAAATCTGTTAGAGTTATTATATTAGTATAACTCTAATTATAT

mRNA sequence

ATGAACAAGAGGGCCAGAATTGGTCATTCCAATAAGGCTGTGGTCAATGTCTGGAAGCGGGAGGTCGGCGAGCTCTCCTCTAGAGATTTCGCCCACCGTCTTGGTGCCTCGGAGGATCTCGTGCTCCGACTTGATCTTTTCAGGAAATTGGAGAGGCACAAAGGTTGTGTGAATACTGTGAGCTTTAGTGCTGCTGGTGACATTCTAGTGTCAGGCTCTGATGATAGGCGGGTGATCCTCTGGAATTGGGAAACCGGGCATGCCAAACTCTGTTTTCAGTCTGGTCATAATAATAATGTTTTCCAAGCAAAAATCATGCCTTACACGGATGACAATAGCATTGTGACTTGTGCTGCGGATGGGCAGGTGAGACATGCTCAGATTCTGGATGGTGGGGATGTGGAAACTGCATTACTTGGCAGTCATCAAGGGCGTGCTCATAAGTTGGCCATTGAACCTGGGAGCCCTTATATCTTTTATACTTGTGGTGAAGATGGATTGGTTCAACATTTTGATCTAAGAACTGGTGTTGCCATTGAGCTGTTCACTTGCCAGTCAGTTGACAAGAGGGCAGGTTACATGTCATCGATTCAGCTAAATGCAATTGCTATTGATCCCAGAAATCCAAATCTCTTTGTCGTTGCTGGTTCAGATGAGTATGCACGGTTGTATGATATTCGCAAATATAAGGGGGAAGGATCAACTGATTTTGGTCAACCTGCAGACTGCTATTGCCCCCCTCACTTGATTGGTGATGAACAAGTGGGAATAACAGGTTTAGCCTTCTCAGAGCTGAGTGAACTCCTTGTTTCATACAATGATGAATCTATTTATCTCTTTAATAGGGATATGGGTTTGGGCCGAAGCCCATATCCATTGTCTCCAGGTACAAGTGATATGGGAGCTGAAAAAGTTAAGGATGGTAGTCCACAAGTTTATAAAGGACATAGAAACTGTGAGACAGTAAAAGGTGTCAATTTTTTTGGACCTAAATGTGAGTATGTCATCAGTGGATCTGATTGTGGCAGAATATTTATATGGAGGAAAAAGGGTGGAGTGCTAATCCGAGTCATGGAAGCAGACGAGGATGTGGTGAACTGCATTGAGCCCCATCCTCACATGACCATGCTTGCTAGTAGTGGAATTGAAAGTGACGTCAAGTTATGGACTCCAAAGGCCCTTGAAAGGGCTACTTTGCCTGAGAAAATTGAACAGAACATAGGTTTTGTGATGATTGGTATTGTTTTCAATGATGATTACAAAGATGATGAAGACAATGAAGGCAACGATGATAAGGGGAAAAAACTGAAGCCTAGGGGATGGATGCACCGGTTAGCTTTGCCGGAGGAACTGATGATGCAACTGTTTTCACTGCGAAGACAGAATTCCAGCCCTGAACACCGAAGAGAAAACTCCGCTGTAGGTAGGGAAATTTTAGAGTTGATAATGACATTCAATAACAACACCGAGAGCTCTTCAGAAGACAACGGTGGTGATACTGCTGAAGATGATTGA

Coding sequence (CDS)

ATGAACAAGAGGGCCAGAATTGGTCATTCCAATAAGGCTGTGGTCAATGTCTGGAAGCGGGAGGTCGGCGAGCTCTCCTCTAGAGATTTCGCCCACCGTCTTGGTGCCTCGGAGGATCTCGTGCTCCGACTTGATCTTTTCAGGAAATTGGAGAGGCACAAAGGTTGTGTGAATACTGTGAGCTTTAGTGCTGCTGGTGACATTCTAGTGTCAGGCTCTGATGATAGGCGGGTGATCCTCTGGAATTGGGAAACCGGGCATGCCAAACTCTGTTTTCAGTCTGGTCATAATAATAATGTTTTCCAAGCAAAAATCATGCCTTACACGGATGACAATAGCATTGTGACTTGTGCTGCGGATGGGCAGGTGAGACATGCTCAGATTCTGGATGGTGGGGATGTGGAAACTGCATTACTTGGCAGTCATCAAGGGCGTGCTCATAAGTTGGCCATTGAACCTGGGAGCCCTTATATCTTTTATACTTGTGGTGAAGATGGATTGGTTCAACATTTTGATCTAAGAACTGGTGTTGCCATTGAGCTGTTCACTTGCCAGTCAGTTGACAAGAGGGCAGGTTACATGTCATCGATTCAGCTAAATGCAATTGCTATTGATCCCAGAAATCCAAATCTCTTTGTCGTTGCTGGTTCAGATGAGTATGCACGGTTGTATGATATTCGCAAATATAAGGGGGAAGGATCAACTGATTTTGGTCAACCTGCAGACTGCTATTGCCCCCCTCACTTGATTGGTGATGAACAAGTGGGAATAACAGGTTTAGCCTTCTCAGAGCTGAGTGAACTCCTTGTTTCATACAATGATGAATCTATTTATCTCTTTAATAGGGATATGGGTTTGGGCCGAAGCCCATATCCATTGTCTCCAGGTACAAGTGATATGGGAGCTGAAAAAGTTAAGGATGGTAGTCCACAAGTTTATAAAGGACATAGAAACTGTGAGACAGTAAAAGGTGTCAATTTTTTTGGACCTAAATGTGAGTATGTCATCAGTGGATCTGATTGTGGCAGAATATTTATATGGAGGAAAAAGGGTGGAGTGCTAATCCGAGTCATGGAAGCAGACGAGGATGTGGTGAACTGCATTGAGCCCCATCCTCACATGACCATGCTTGCTAGTAGTGGAATTGAAAGTGACGTCAAGTTATGGACTCCAAAGGCCCTTGAAAGGGCTACTTTGCCTGAGAAAATTGAACAGAACATAGGTTTTGTGATGATTGGTATTGTTTTCAATGATGATTACAAAGATGATGAAGACAATGAAGGCAACGATGATAAGGGGAAAAAACTGAAGCCTAGGGGATGGATGCACCGGTTAGCTTTGCCGGAGGAACTGATGATGCAACTGTTTTCACTGCGAAGACAGAATTCCAGCCCTGAACACCGAAGAGAAAACTCCGCTGTAGGTAGGGAAATTTTAGAGTTGATAATGACATTCAATAACAACACCGAGAGCTCTTCAGAAGACAACGGTGGTGATACTGCTGAAGATGATTGA

Protein sequence

MNKRARIGHSNKAVVNVWKREVGELSSRDFAHRLGASEDLVLRLDLFRKLERHKGCVNTVSFSAAGDILVSGSDDRRVILWNWETGHAKLCFQSGHNNNVFQAKIMPYTDDNSIVTCAADGQVRHAQILDGGDVETALLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRTGVAIELFTCQSVDKRAGYMSSIQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQPADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLGRSPYPLSPGTSDMGAEKVKDGSPQVYKGHRNCETVKGVNFFGPKCEYVISGSDCGRIFIWRKKGGVLIRVMEADEDVVNCIEPHPHMTMLASSGIESDVKLWTPKALERATLPEKIEQNIGFVMIGIVFNDDYKDDEDNEGNDDKGKKLKPRGWMHRLALPEELMMQLFSLRRQNSSPEHRRENSAVGREILELIMTFNNNTESSSEDNGGDTAEDD
Homology
BLAST of Spg014981 vs. NCBI nr
Match: XP_022136068.1 (DDB1- and CUL4-associated factor 8 [Momordica charantia])

HSP 1 Score: 864.0 bits (2231), Expect = 6.4e-247
Identity = 421/509 (82.71%), Postives = 448/509 (88.02%), Query Frame = 0

Query: 1   MNKRARIGHSNKAVVNVWKREVGELSSRDFAHRLGASEDLVLRLDLFRKLERHKGCVNTV 60
           MNKRARI  SNKA+VNVWKREVGELS+R+FAHRLGAS+DLVLRLD++RKLERHKGCVNTV
Sbjct: 1   MNKRARIDDSNKALVNVWKREVGELSTRNFAHRLGASQDLVLRLDIYRKLERHKGCVNTV 60

Query: 61  SFSAAGDILVSGSDDRRVILWNWETGHAKLCFQSGHNNNVFQAKIMPYTDDNSIVTCAAD 120
           SF+AAGD+LVSGSDDRRVILWNWETG  KL FQSGHNNNVFQAKIMPYTDD  IVTCAAD
Sbjct: 61  SFNAAGDVLVSGSDDRRVILWNWETGQVKLSFQSGHNNNVFQAKIMPYTDDRCIVTCAAD 120

Query: 121 GQVRHAQILDGGDVETALLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRTGVAIE 180
           GQVRHAQIL+ G VET+LLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRTG A E
Sbjct: 121 GQVRHAQILNSGRVETSLLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRTGAASE 180

Query: 181 LFTCQSVDKRAGYMSSIQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQP 240
           LFTCQS+D RAGYMSSIQLNAI IDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQP
Sbjct: 181 LFTCQSIDNRAGYMSSIQLNAITIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQP 240

Query: 241 ADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLGRSPYPLSP----- 300
            D YCPPHLIGDEQVGITGLA+SELSELLVSYNDESIYLFNRDMGLG +P P+SP     
Sbjct: 241 TDYYCPPHLIGDEQVGITGLAYSELSELLVSYNDESIYLFNRDMGLGPNPNPVSPVSMSS 300

Query: 301 GTSDMGAEKVKDGSPQVYKGHRNCETVKGVNFFGPKCEYVISGSDCGRIFIWRKKGGVLI 360
            +S+MGAE V+ GSPQVYKGHRNCETVKGVNFFGPKCEYV+SGSDCGRIFIW+KKGG LI
Sbjct: 301 DSSEMGAENVEHGSPQVYKGHRNCETVKGVNFFGPKCEYVVSGSDCGRIFIWKKKGGELI 360

Query: 361 RVMEADEDVVNCIEPHPHMTMLASSGIESDVKLWTPKALERATLPEKIEQNIGFVMIGIV 420
           RVMEADEDVVNCIEPHPH+TMLASSGIESD+K+WTPKALERATLPEKIEQ          
Sbjct: 361 RVMEADEDVVNCIEPHPHVTMLASSGIESDIKIWTPKALERATLPEKIEQ---------- 420

Query: 421 FNDDYKDDEDNEGNDDKGKKLKPRGWMHRLALPEELMMQLFSLRRQNSSPEHRRENSAVG 480
                              K KPRGWMHRLALPEELMMQLFSLRR+NSSPE RRENSAV 
Sbjct: 421 -------------------KPKPRGWMHRLALPEELMMQLFSLRRRNSSPERRRENSAVS 480

Query: 481 REILELIMTFNNNTESSSEDNGGDTAEDD 505
           REILELI+TFN N++ SS+DNG DTAEDD
Sbjct: 481 REILELILTFNANSDESSDDNGDDTAEDD 480

BLAST of Spg014981 vs. NCBI nr
Match: XP_038887987.1 (DDB1- and CUL4-associated factor 8-like [Benincasa hispida])

HSP 1 Score: 833.2 bits (2151), Expect = 1.2e-237
Identity = 417/510 (81.76%), Postives = 435/510 (85.29%), Query Frame = 0

Query: 1   MNKRARIGHSNKAVVNVWKREVGELSSRDFAHRLGASEDLVLRLDLFRKLERHKGCVNTV 60
           MNKRARI  SNKAVVNVWKREVGELS+R+FAHRLGA EDLVLRLDL+RKLERHKGCVNTV
Sbjct: 1   MNKRARIDDSNKAVVNVWKREVGELSTRNFAHRLGALEDLVLRLDLYRKLERHKGCVNTV 60

Query: 61  SFSAAGDILVSGSDDRRVILWNWETGHAKLCFQSGHNNNVFQAKIMPYTDDNSIVTCAAD 120
           SF+AAGDILVSGSDDRRVILWNWETGH KL FQSGHNNNVFQAKIMPYTDD SIVTCAAD
Sbjct: 61  SFNAAGDILVSGSDDRRVILWNWETGHVKLSFQSGHNNNVFQAKIMPYTDDRSIVTCAAD 120

Query: 121 GQVRHAQILDGGDVETALLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRTGVAIE 180
           GQVRHAQIL+ G VETALLGSH GRAHKLA EPGSPY+FYTCGEDGLVQ FDLRTG A+E
Sbjct: 121 GQVRHAQILNSGRVETALLGSHLGRAHKLAFEPGSPYMFYTCGEDGLVQRFDLRTGAAVE 180

Query: 181 LFTCQSVDKRAGYMSSIQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQP 240
           LFTCQSVD RAGYMSSIQLNAIAIDPRNPNLFVVAGSDEYARLYDIRK   +GSTDFGQ 
Sbjct: 181 LFTCQSVDNRAGYMSSIQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKSGEDGSTDFGQL 240

Query: 241 ADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLGRSPYPLSP----- 300
            DCYCPPHLIGDEQVGITGLAFSE+SELLVSYNDESIYLFNRDMGLG +P P SP     
Sbjct: 241 VDCYCPPHLIGDEQVGITGLAFSEMSELLVSYNDESIYLFNRDMGLGPNPNPASPVSLSS 300

Query: 301 GTSDMGAEKVKDGSPQVYKGHRNCETVKGVNFFGPKCEYVISGSDCGRIFIWRKKGGVLI 360
            TS+M AE V++ SPQVYKGHRNCETVKGVNFFGPKCEYV+SGSDCGRIFIWRKKGG LI
Sbjct: 301 DTSEMEAENVENVSPQVYKGHRNCETVKGVNFFGPKCEYVVSGSDCGRIFIWRKKGGELI 360

Query: 361 RVMEADEDVVNCIEPHPHMTMLASSGIESDVKLWTPKALERATLPEKIEQNIGFVMIGIV 420
           RVMEADEDVVNCIE HPHMTMLASSGIESD+K+WTPKALERATLPEKIEQ          
Sbjct: 361 RVMEADEDVVNCIESHPHMTMLASSGIESDIKIWTPKALERATLPEKIEQ---------- 420

Query: 421 FNDDYKDDEDNEGNDDKGKKLKPRGWMHRLALPEELMMQLFSLRRQNSSPEHRRENSAVG 480
                              K KPRGWMHRLALPEELMMQLFSLRRQ  SPE  RE   V 
Sbjct: 421 -------------------KPKPRGWMHRLALPEELMMQLFSLRRQIMSPERGREPPTVS 480

Query: 481 REILELIMTFNN-NTESSSEDNGGDTAEDD 505
           REILELIMTFNN N++ SS  NG DTAEDD
Sbjct: 481 REILELIMTFNNGNSDDSSYYNGDDTAEDD 481

BLAST of Spg014981 vs. NCBI nr
Match: XP_008447917.1 (PREDICTED: DDB1- and CUL4-associated factor 8 [Cucumis melo])

HSP 1 Score: 832.8 bits (2150), Expect = 1.6e-237
Identity = 414/509 (81.34%), Postives = 430/509 (84.48%), Query Frame = 0

Query: 1   MNKRARIGHSNKAVVNVWKREVGELSSRDFAHRLGASEDLVLRLDLFRKLERHKGCVNTV 60
           MNKRARI  SNKAVVNVWKREVGELS+R+FAHRLGA EDLVLRLDL++KLERHKGCVNTV
Sbjct: 1   MNKRARIDDSNKAVVNVWKREVGELSTRNFAHRLGALEDLVLRLDLYKKLERHKGCVNTV 60

Query: 61  SFSAAGDILVSGSDDRRVILWNWETGHAKLCFQSGHNNNVFQAKIMPYTDDNSIVTCAAD 120
           SF+AAGDILVSGSDDRRVILWNWETG  KL FQSGHNNNVFQAKIMPYTDD SIVTCAAD
Sbjct: 61  SFNAAGDILVSGSDDRRVILWNWETGRVKLSFQSGHNNNVFQAKIMPYTDDRSIVTCAAD 120

Query: 121 GQVRHAQILDGGDVETALLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRTGVAIE 180
           GQVRHAQIL+ G VET+LLGSH GRAHKLAIEPGSPY+FYTCGEDGLVQ FDLRTG A+E
Sbjct: 121 GQVRHAQILNSGHVETSLLGSHLGRAHKLAIEPGSPYMFYTCGEDGLVQRFDLRTGAAVE 180

Query: 181 LFTCQSVDKRAGYMSSIQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQP 240
           LFTCQSVD RAGYMSSIQLNAI IDPRNPNLFVVAGSDEYARLYDIRK   +GSTDFGQ 
Sbjct: 181 LFTCQSVDNRAGYMSSIQLNAIVIDPRNPNLFVVAGSDEYARLYDIRKSGEDGSTDFGQL 240

Query: 241 ADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLGRSPYP-----LSP 300
           ADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLG +P P     LS 
Sbjct: 241 ADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLGPNPNPAPPLSLSS 300

Query: 301 GTSDMGAEKVKDGSPQVYKGHRNCETVKGVNFFGPKCEYVISGSDCGRIFIWRKKGGVLI 360
             S+MGAEKV +G PQVYKGHRNCETVKGVNFFGPKCEYV+SGSDCGRIFIWRKKGG LI
Sbjct: 301 DASEMGAEKVDNGIPQVYKGHRNCETVKGVNFFGPKCEYVVSGSDCGRIFIWRKKGGELI 360

Query: 361 RVMEADEDVVNCIEPHPHMTMLASSGIESDVKLWTPKALERATLPEKIEQNIGFVMIGIV 420
           RVMEADEDVVNCIEPHPHMTMLASSGIESDVK+WTPKA ERATLPEKIEQ          
Sbjct: 361 RVMEADEDVVNCIEPHPHMTMLASSGIESDVKIWTPKAHERATLPEKIEQ---------- 420

Query: 421 FNDDYKDDEDNEGNDDKGKKLKPRGWMHRLALPEELMMQLFSLRRQNSSPEHRRENSAVG 480
                              K KPRGWMHRLALPEELMMQLFSLRRQ SSPE  RE   V 
Sbjct: 421 -------------------KPKPRGWMHRLALPEELMMQLFSLRRQTSSPERGREPPTVS 480

Query: 481 REILELIMTFNNNTESSSEDNGGDTAEDD 505
           RE+LELIMTFNN     S D   DT EDD
Sbjct: 481 RELLELIMTFNNGNSDDSSDYNDDTGEDD 480

BLAST of Spg014981 vs. NCBI nr
Match: TYK23393.1 (DDB1- and CUL4-associated factor 8 [Cucumis melo var. makuwa])

HSP 1 Score: 827.4 bits (2136), Expect = 6.7e-236
Identity = 412/509 (80.94%), Postives = 428/509 (84.09%), Query Frame = 0

Query: 1   MNKRARIGHSNKAVVNVWKREVGELSSRDFAHRLGASEDLVLRLDLFRKLERHKGCVNTV 60
           MNKRARI  SNKAVVNVWKREVGELS+R+FAHRLGA EDLVLRLDL++KLERHKGCVNTV
Sbjct: 1   MNKRARIDDSNKAVVNVWKREVGELSTRNFAHRLGALEDLVLRLDLYKKLERHKGCVNTV 60

Query: 61  SFSAAGDILVSGSDDRRVILWNWETGHAKLCFQSGHNNNVFQAKIMPYTDDNSIVTCAAD 120
           SF+AAGDILVSGSDDRRVILWNWETG  KL FQSGHNNNVFQAKIMPYTDD SIVTCAAD
Sbjct: 61  SFNAAGDILVSGSDDRRVILWNWETGRVKLSFQSGHNNNVFQAKIMPYTDDRSIVTCAAD 120

Query: 121 GQVRHAQILDGGDVETALLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRTGVAIE 180
           GQVRHAQIL+ G VET+LLGSH GRAHKLAIEPGSPY+FYTCGEDGLVQ FDLRTG A+E
Sbjct: 121 GQVRHAQILNSGHVETSLLGSHLGRAHKLAIEPGSPYMFYTCGEDGLVQRFDLRTGAAVE 180

Query: 181 LFTCQSVDKRAGYMSSIQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQP 240
           LFTCQSVD RAGYMSSIQLNAI IDPRNPNLFVVAGSDEYARLYDIRK   +GSTDFGQ 
Sbjct: 181 LFTCQSVDNRAGYMSSIQLNAIVIDPRNPNLFVVAGSDEYARLYDIRKSGEDGSTDFGQL 240

Query: 241 ADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLGRSPYP-----LSP 300
            D YCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLG +P P     LS 
Sbjct: 241 VDSYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLGPNPNPAPPLSLSS 300

Query: 301 GTSDMGAEKVKDGSPQVYKGHRNCETVKGVNFFGPKCEYVISGSDCGRIFIWRKKGGVLI 360
             S+MGAEKV +G PQVYKGHRNCETVKGVNFFGPKCEYV+SGSDCGRIFIWRKKGG LI
Sbjct: 301 DASEMGAEKVDNGIPQVYKGHRNCETVKGVNFFGPKCEYVVSGSDCGRIFIWRKKGGELI 360

Query: 361 RVMEADEDVVNCIEPHPHMTMLASSGIESDVKLWTPKALERATLPEKIEQNIGFVMIGIV 420
           RVMEADEDVVNCIEPHPHMTMLASSGIESDVK+WTPKA ERATLPEKIEQ          
Sbjct: 361 RVMEADEDVVNCIEPHPHMTMLASSGIESDVKIWTPKAHERATLPEKIEQ---------- 420

Query: 421 FNDDYKDDEDNEGNDDKGKKLKPRGWMHRLALPEELMMQLFSLRRQNSSPEHRRENSAVG 480
                              K KPRGWMHRLALPEELMMQLFSLRRQ SSPE  RE   V 
Sbjct: 421 -------------------KPKPRGWMHRLALPEELMMQLFSLRRQTSSPERGREPPTVS 480

Query: 481 REILELIMTFNNNTESSSEDNGGDTAEDD 505
           RE+LELIMTFNN     S D   DT EDD
Sbjct: 481 RELLELIMTFNNGNSDDSSDYNDDTGEDD 480

BLAST of Spg014981 vs. NCBI nr
Match: XP_004144864.1 (DDB1- and CUL4-associated factor 8 [Cucumis sativus] >KGN43277.1 hypothetical protein Csa_020173 [Cucumis sativus])

HSP 1 Score: 823.9 bits (2127), Expect = 7.4e-235
Identity = 410/509 (80.55%), Postives = 428/509 (84.09%), Query Frame = 0

Query: 1   MNKRARIGHSNKAVVNVWKREVGELSSRDFAHRLGASEDLVLRLDLFRKLERHKGCVNTV 60
           MNKRARI  SNKAVVNVWKREVGELS+R+FAHRLGA EDLVLRLDL++KLERHKGCVNTV
Sbjct: 1   MNKRARIDDSNKAVVNVWKREVGELSTRNFAHRLGALEDLVLRLDLYKKLERHKGCVNTV 60

Query: 61  SFSAAGDILVSGSDDRRVILWNWETGHAKLCFQSGHNNNVFQAKIMPYTDDNSIVTCAAD 120
           SF+AAGDILVSGSDDRRVILWNWETG  KL FQSGHNNNVFQAKIMPYTDD SIVTCAAD
Sbjct: 61  SFNAAGDILVSGSDDRRVILWNWETGRVKLSFQSGHNNNVFQAKIMPYTDDQSIVTCAAD 120

Query: 121 GQVRHAQILDGGDVETALLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRTGVAIE 180
           GQVRHAQIL+ G VET+LLG+H GRAHKLAIEPGSPY+FYTCGEDGLVQ FDLRTG A+E
Sbjct: 121 GQVRHAQILNSGHVETSLLGNHLGRAHKLAIEPGSPYMFYTCGEDGLVQRFDLRTGDAVE 180

Query: 181 LFTCQSVDKRAGYMSSIQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQP 240
           LFTCQSVD RAGYMSSIQLNAI IDPRNPNLFVVAGSDEYARLYDIRK   +GSTDFGQ 
Sbjct: 181 LFTCQSVDNRAGYMSSIQLNAIVIDPRNPNLFVVAGSDEYARLYDIRKSGEDGSTDFGQL 240

Query: 241 ADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLGRSPYP-----LSP 300
           ADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLG +P P     LS 
Sbjct: 241 ADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLGPNPNPVPPLSLSS 300

Query: 301 GTSDMGAEKVKDGSPQVYKGHRNCETVKGVNFFGPKCEYVISGSDCGRIFIWRKKGGVLI 360
             S+MGAE V +G PQVYKGHRNCETVKGVNFFGPKCEYV+SGSDCGRIFIWRKKGG LI
Sbjct: 301 DASEMGAENVDNGIPQVYKGHRNCETVKGVNFFGPKCEYVVSGSDCGRIFIWRKKGGDLI 360

Query: 361 RVMEADEDVVNCIEPHPHMTMLASSGIESDVKLWTPKALERATLPEKIEQNIGFVMIGIV 420
           RVMEADEDVVNCIEPHPHMTMLASSGIESDVK+WTPKA ERATLPEKIEQ          
Sbjct: 361 RVMEADEDVVNCIEPHPHMTMLASSGIESDVKIWTPKAHERATLPEKIEQ---------- 420

Query: 421 FNDDYKDDEDNEGNDDKGKKLKPRGWMHRLALPEELMMQLFSLRRQNSSPEHRRENSAVG 480
                              K KPRGWMHRLALPEELMMQLFSLRRQ SSPE  RE   V 
Sbjct: 421 -------------------KPKPRGWMHRLALPEELMMQLFSLRRQTSSPESGREPPTVS 480

Query: 481 REILELIMTFNNNTESSSEDNGGDTAEDD 505
           RE+LELI TFNN     S D   D+ EDD
Sbjct: 481 RELLELIRTFNNGNSDDSSDYNDDSGEDD 480

BLAST of Spg014981 vs. ExPASy Swiss-Prot
Match: Q8N7N5 (DDB1- and CUL4-associated factor 8 OS=Mus musculus OX=10090 GN=Dcaf8 PE=1 SV=1)

HSP 1 Score: 296.6 bits (758), Expect = 5.4e-79
Identity = 167/396 (42.17%), Postives = 229/396 (57.83%), Query Frame = 0

Query: 19  KREVGELSSRDFAHRLGASEDLVLRLDLFRKLERHKGCVNTVSFSAAGDILVSGSDDRRV 78
           +RE+G  SS  F +    +   V R  L   LE H GCVNT+ F+  G  L SGSDD +V
Sbjct: 154 ERELG--SSARFVYEACGARVFVQRFRLQHGLEGHTGCVNTLHFNQRGTWLASGSDDLKV 213

Query: 79  ILWNWETGHAKLCFQSGHNNNVFQAKIMPYTDDNSIVTCAADGQVRHAQILDGGDVE-TA 138
           ++W+W      L F+SGH +NVFQAK +P + D+++  CA DGQVR A++      + T 
Sbjct: 214 VVWDWVRRQPVLDFESGHKSNVFQAKFLPNSGDSTLAMCARDGQVRVAELSATQCCKNTK 273

Query: 139 LLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRTG-VAIELFTCQSVDKRAGYMSS 198
            +  H+G +HKLA+EP SP  F + GED +V   DLR    A +L   +  +K+ G    
Sbjct: 274 RVAQHKGASHKLALEPDSPCTFLSAGEDAVVFTIDLRQDRPASKLVVTKEKEKKVG---- 333

Query: 199 IQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQPADCYCPPHLIGDE-QV 258
             L  I ++P N + F V G D+Y R+YD RK     +    +    +CP HL+  E + 
Sbjct: 334 --LYTIYVNPANTHQFAVGGRDQYVRIYDQRKIDENENNGVLKK---FCPHHLVNSESKA 393

Query: 259 GITGLAFS-ELSELLVSYNDESIYLFNRDMGLGRSPYPLSPGTSDMGAEKVKDGSPQVYK 318
            IT L +S + +ELL SYNDE IYLFN               +   GA+ +K      YK
Sbjct: 394 NITCLVYSHDGTELLASYNDEDIYLFN--------------SSHSDGAQYIKR-----YK 453

Query: 319 GHRNCETVKGVNFFGPKCEYVISGSDCGRIFIWRKKGGVLIRVMEADE-DVVNCIEPHPH 378
           GHRN  TVKGVNF+GPK E+V+SGSDCG IF+W K    +I+ ME D+  VVNC+EPHPH
Sbjct: 454 GHRNNATVKGVNFYGPKSEFVVSGSDCGHIFLWEKSSCQIIQFMEGDKGGVVNCLEPHPH 513

Query: 379 MTMLASSGIESDVKLWTPKA---LERATLPEKIEQN 407
           + +LA+SG++ DVK+W P A    E   L E I++N
Sbjct: 514 LPVLATSGLDHDVKIWAPTAEASTELTGLKEVIKKN 519

BLAST of Spg014981 vs. ExPASy Swiss-Prot
Match: Q5TAQ9 (DDB1- and CUL4-associated factor 8 OS=Homo sapiens OX=9606 GN=DCAF8 PE=1 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 2.7e-78
Identity = 166/396 (41.92%), Postives = 229/396 (57.83%), Query Frame = 0

Query: 19  KREVGELSSRDFAHRLGASEDLVLRLDLFRKLERHKGCVNTVSFSAAGDILVSGSDDRRV 78
           +RE+G  SS  F +    +   V R  L   LE H GCVNT+ F+  G  L SGSDD +V
Sbjct: 160 ERELG--SSARFVYEACGARVFVQRFRLQHGLEGHTGCVNTLHFNQRGTWLASGSDDLKV 219

Query: 79  ILWNWETGHAKLCFQSGHNNNVFQAKIMPYTDDNSIVTCAADGQVRHAQILDGGDVE-TA 138
           ++W+W      L F+SGH +NVFQAK +P + D+++  CA DGQVR A++      + T 
Sbjct: 220 VVWDWVRRQPVLDFESGHKSNVFQAKFLPNSGDSTLAMCARDGQVRVAELSATQCCKNTK 279

Query: 139 LLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRTG-VAIELFTCQSVDKRAGYMSS 198
            +  H+G +HKLA+EP SP  F + GED +V   DLR    A +L   +  +K+ G    
Sbjct: 280 RVAQHKGASHKLALEPDSPCTFLSAGEDAVVFTIDLRQDRPASKLVVTKEKEKKVG---- 339

Query: 199 IQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQPADCYCPPHLIGDE-QV 258
             L  I ++P N + F V G D++ R+YD RK     +    +    +CP HL+  E + 
Sbjct: 340 --LYTIYVNPANTHQFAVGGRDQFVRIYDQRKIDENENNGVLKK---FCPHHLVNSESKA 399

Query: 259 GITGLAFS-ELSELLVSYNDESIYLFNRDMGLGRSPYPLSPGTSDMGAEKVKDGSPQVYK 318
            IT L +S + +ELL SYNDE IYLFN               +   GA+ VK      YK
Sbjct: 400 NITCLVYSHDGTELLASYNDEDIYLFN--------------SSHSDGAQYVKR-----YK 459

Query: 319 GHRNCETVKGVNFFGPKCEYVISGSDCGRIFIWRKKGGVLIRVMEADE-DVVNCIEPHPH 378
           GHRN  TVKGVNF+GPK E+V+SGSDCG IF+W K    +I+ ME D+  VVNC+EPHPH
Sbjct: 460 GHRNNATVKGVNFYGPKSEFVVSGSDCGHIFLWEKSSCQIIQFMEGDKGGVVNCLEPHPH 519

Query: 379 MTMLASSGIESDVKLWTPKA---LERATLPEKIEQN 407
           + +LA+SG++ DVK+W P A    E   L + I++N
Sbjct: 520 LPVLATSGLDHDVKIWAPTAEASTELTGLKDVIKKN 525

BLAST of Spg014981 vs. ExPASy Swiss-Prot
Match: Q5U2M6 (DDB1- and CUL4-associated factor 8 OS=Rattus norvegicus OX=10116 GN=Dcaf8 PE=1 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 3.5e-78
Identity = 165/396 (41.67%), Postives = 229/396 (57.83%), Query Frame = 0

Query: 19  KREVGELSSRDFAHRLGASEDLVLRLDLFRKLERHKGCVNTVSFSAAGDILVSGSDDRRV 78
           +RE+G  SS  F +    +   V R  L   LE H GCVNT+ F+  G  L SGSDD +V
Sbjct: 154 ERELG--SSARFVYEACGARVFVQRFRLQHGLEGHTGCVNTLHFNQRGTWLASGSDDLKV 213

Query: 79  ILWNWETGHAKLCFQSGHNNNVFQAKIMPYTDDNSIVTCAADGQVRHAQILDGGDVE-TA 138
           ++W+W      L F+SGH +NVFQAK +P + D+++  CA DGQVR A++      + T 
Sbjct: 214 VVWDWVRRQPVLDFESGHKSNVFQAKFLPNSGDSTLAMCARDGQVRVAELSATQCCKNTK 273

Query: 139 LLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRTG-VAIELFTCQSVDKRAGYMSS 198
            +  H+G +HKLA+EP SP  F + GED +V   DLR    A +L   +  +K+ G    
Sbjct: 274 RVAQHKGASHKLALEPDSPCTFLSAGEDAVVFTIDLRQDRPASKLVVTKEKEKKVG---- 333

Query: 199 IQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQPADCYCPPHLIGDE-QV 258
             L  I ++P N + F V G D++ R+YD RK     +    +    +CP HL+  E + 
Sbjct: 334 --LYTIYVNPANTHQFAVGGRDQFVRIYDQRKIDENENNGVLKK---FCPHHLVNSESKA 393

Query: 259 GITGLAFS-ELSELLVSYNDESIYLFNRDMGLGRSPYPLSPGTSDMGAEKVKDGSPQVYK 318
            IT L +S + +ELL SYNDE IYLFN               +   GA+ +K      YK
Sbjct: 394 NITCLVYSHDGTELLASYNDEDIYLFN--------------SSHSDGAQYIKR-----YK 453

Query: 319 GHRNCETVKGVNFFGPKCEYVISGSDCGRIFIWRKKGGVLIRVMEADE-DVVNCIEPHPH 378
           GHRN  TVKGVNF+GPK E+V+SGSDCG IF+W K    +I+ ME D+  VVNC+EPHPH
Sbjct: 454 GHRNNATVKGVNFYGPKSEFVVSGSDCGHIFLWEKSSCQIIQFMEGDKGGVVNCLEPHPH 513

Query: 379 MTMLASSGIESDVKLWTPKA---LERATLPEKIEQN 407
           + +LA+SG++ DVK+W P A    E   L + I++N
Sbjct: 514 LPVLATSGLDHDVKIWAPTAEASTELTGLKDVIKKN 519

BLAST of Spg014981 vs. ExPASy Swiss-Prot
Match: Q5R448 (DDB1- and CUL4-associated factor 8 OS=Pongo abelii OX=9601 GN=DCAF8 PE=2 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 2.9e-77
Identity = 165/396 (41.67%), Postives = 229/396 (57.83%), Query Frame = 0

Query: 19  KREVGELSSRDFAHRLGASEDLVLRLDLFRKLERHKGCVNTVSFSAAGDILVSGSDDRRV 78
           +RE+G  SS  F +    +   V R  L   LE H GCVNT+ F+  G  L SGSDD +V
Sbjct: 160 ERELG--SSARFVYEACGARVFVQRFRLQHGLEGHTGCVNTLHFNQRGTWLASGSDDLKV 219

Query: 79  ILWNWETGHAKLCFQSGHNNNVFQAKIMPYTDDNSIVTCAADGQVRHAQILDGGDVE-TA 138
           ++W+W      L F+SGH +NVFQAK +P + D+++   A DGQVR A++      + T 
Sbjct: 220 VVWDWVRRQPVLDFESGHKSNVFQAKFLPNSGDSTLAMRARDGQVRVAELSATQCCKNTK 279

Query: 139 LLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRTG-VAIELFTCQSVDKRAGYMSS 198
            +  H+G +HKLA+EP SP  F + GED +V   DLR    A +L   +  +K+ G    
Sbjct: 280 RVAQHKGASHKLALEPDSPCTFLSAGEDAVVFTIDLRQDRPASKLVVTKEKEKKVG---- 339

Query: 199 IQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQPADCYCPPHLI-GDEQV 258
             L  I ++P N + F V G D++ R+YD RK     +    +    +CP HL+ G+ + 
Sbjct: 340 --LYTIYVNPANTHQFAVGGRDQFVRIYDQRKIDENENNGVLKK---FCPHHLVNGESKA 399

Query: 259 GITGLAFS-ELSELLVSYNDESIYLFNRDMGLGRSPYPLSPGTSDMGAEKVKDGSPQVYK 318
            IT L +S + +ELL SYNDE IYLFN               +   GA+ VK      YK
Sbjct: 400 NITCLVYSHDGTELLASYNDEDIYLFN--------------SSHSDGAQYVKR-----YK 459

Query: 319 GHRNCETVKGVNFFGPKCEYVISGSDCGRIFIWRKKGGVLIRVMEADE-DVVNCIEPHPH 378
           GHRN  TVKGVNF+GPK E+V+SGSDCG IF+W K    +I+ ME D+  VVNC+EPHPH
Sbjct: 460 GHRNNATVKGVNFYGPKSEFVVSGSDCGHIFLWEKSSCQIIQFMEGDKGGVVNCLEPHPH 519

Query: 379 MTMLASSGIESDVKLWTPKA---LERATLPEKIEQN 407
           + +LA+SG++ DVK+W P A    E   L + I++N
Sbjct: 520 LPVLATSGLDHDVKIWAPTAEASTELTGLKDVIKKN 525

BLAST of Spg014981 vs. ExPASy Swiss-Prot
Match: Q6NRH1 (DDB1- and CUL4-associated factor 8 OS=Xenopus laevis OX=8355 GN=dcaf8 PE=2 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 3.8e-77
Identity = 160/393 (40.71%), Postives = 225/393 (57.25%), Query Frame = 0

Query: 12  KAVVNVWKREVGELSSRDFAHRLGASEDLVLRLDLFRKLERHKGCVNTVSFSAAGDILVS 71
           +A+  + +R++G  SS  F +    +   V R  L   L+ H GCVNT+ F+  G  L S
Sbjct: 156 RALSALRQRQMG--SSTRFIYEACGARGFVQRFHLLHGLDGHSGCVNTLHFNQRGTCLAS 215

Query: 72  GSDDRRVILWNWETGHAKLCFQSGHNNNVFQAKIMPYTDDNSIVTCAADGQVRHAQILDG 131
           GSDD +V++W+W      L F+SGH +NVFQAK +P + D+++  CA DGQVR A++   
Sbjct: 216 GSDDLKVVVWDWVRRKPVLEFESGHKSNVFQAKFLPNSGDSTLAMCARDGQVRVAELSAT 275

Query: 132 GDVE-TALLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRTG-VAIELFTCQSVDK 191
              + T  +  H+G +HKLA+EP SP  F + GED +V   DLR    A  L   +  + 
Sbjct: 276 HCCKNTKRVAQHKGASHKLALEPDSPCTFLSAGEDAVVFTIDLRQDRPASRLVVTKEKES 335

Query: 192 RAGYMSSIQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQPADCYCPPHL 251
           + G      L  I ++P N   F V G D++ R+YD RK     +    +    +CP HL
Sbjct: 336 KVG------LYTIYVNPANTYQFAVGGRDQFVRIYDQRKINENVNNGVLKK---FCPHHL 395

Query: 252 IGDE-QVGITGLAFS-ELSELLVSYNDESIYLFNRDMGLGRSPYPLSPGTSDMGAEKVKD 311
           +  E +  IT L +S + SELL SYNDE IYLFN               +   GAE +K 
Sbjct: 396 VTSEAKANITCLVYSHDGSELLASYNDEDIYLFN--------------SSHSDGAEYIKR 455

Query: 312 GSPQVYKGHRNCETVKGVNFFGPKCEYVISGSDCGRIFIWRKKGGVLIRVMEADE-DVVN 371
                YKGHRN  TVKGVNF+GP+ E+V+SGSDCG IF+W K    +++ M+ D+  VVN
Sbjct: 456 -----YKGHRNNATVKGVNFYGPRSEFVVSGSDCGHIFLWEKSSCQIVQFMDGDKGGVVN 515

Query: 372 CIEPHPHMTMLASSGIESDVKLWTPKALERATL 400
           C+EPHPH+ +LA+SG++ DVK+W P A E   L
Sbjct: 516 CLEPHPHLPVLATSGLDYDVKIWLPTAKEPTEL 518

BLAST of Spg014981 vs. ExPASy TrEMBL
Match: A0A6J1C6L2 (DDB1- and CUL4-associated factor 8 OS=Momordica charantia OX=3673 GN=LOC111007852 PE=4 SV=1)

HSP 1 Score: 864.0 bits (2231), Expect = 3.1e-247
Identity = 421/509 (82.71%), Postives = 448/509 (88.02%), Query Frame = 0

Query: 1   MNKRARIGHSNKAVVNVWKREVGELSSRDFAHRLGASEDLVLRLDLFRKLERHKGCVNTV 60
           MNKRARI  SNKA+VNVWKREVGELS+R+FAHRLGAS+DLVLRLD++RKLERHKGCVNTV
Sbjct: 1   MNKRARIDDSNKALVNVWKREVGELSTRNFAHRLGASQDLVLRLDIYRKLERHKGCVNTV 60

Query: 61  SFSAAGDILVSGSDDRRVILWNWETGHAKLCFQSGHNNNVFQAKIMPYTDDNSIVTCAAD 120
           SF+AAGD+LVSGSDDRRVILWNWETG  KL FQSGHNNNVFQAKIMPYTDD  IVTCAAD
Sbjct: 61  SFNAAGDVLVSGSDDRRVILWNWETGQVKLSFQSGHNNNVFQAKIMPYTDDRCIVTCAAD 120

Query: 121 GQVRHAQILDGGDVETALLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRTGVAIE 180
           GQVRHAQIL+ G VET+LLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRTG A E
Sbjct: 121 GQVRHAQILNSGRVETSLLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRTGAASE 180

Query: 181 LFTCQSVDKRAGYMSSIQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQP 240
           LFTCQS+D RAGYMSSIQLNAI IDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQP
Sbjct: 181 LFTCQSIDNRAGYMSSIQLNAITIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQP 240

Query: 241 ADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLGRSPYPLSP----- 300
            D YCPPHLIGDEQVGITGLA+SELSELLVSYNDESIYLFNRDMGLG +P P+SP     
Sbjct: 241 TDYYCPPHLIGDEQVGITGLAYSELSELLVSYNDESIYLFNRDMGLGPNPNPVSPVSMSS 300

Query: 301 GTSDMGAEKVKDGSPQVYKGHRNCETVKGVNFFGPKCEYVISGSDCGRIFIWRKKGGVLI 360
            +S+MGAE V+ GSPQVYKGHRNCETVKGVNFFGPKCEYV+SGSDCGRIFIW+KKGG LI
Sbjct: 301 DSSEMGAENVEHGSPQVYKGHRNCETVKGVNFFGPKCEYVVSGSDCGRIFIWKKKGGELI 360

Query: 361 RVMEADEDVVNCIEPHPHMTMLASSGIESDVKLWTPKALERATLPEKIEQNIGFVMIGIV 420
           RVMEADEDVVNCIEPHPH+TMLASSGIESD+K+WTPKALERATLPEKIEQ          
Sbjct: 361 RVMEADEDVVNCIEPHPHVTMLASSGIESDIKIWTPKALERATLPEKIEQ---------- 420

Query: 421 FNDDYKDDEDNEGNDDKGKKLKPRGWMHRLALPEELMMQLFSLRRQNSSPEHRRENSAVG 480
                              K KPRGWMHRLALPEELMMQLFSLRR+NSSPE RRENSAV 
Sbjct: 421 -------------------KPKPRGWMHRLALPEELMMQLFSLRRRNSSPERRRENSAVS 480

Query: 481 REILELIMTFNNNTESSSEDNGGDTAEDD 505
           REILELI+TFN N++ SS+DNG DTAEDD
Sbjct: 481 REILELILTFNANSDESSDDNGDDTAEDD 480

BLAST of Spg014981 vs. ExPASy TrEMBL
Match: A0A1S3BIJ4 (DDB1- and CUL4-associated factor 8 OS=Cucumis melo OX=3656 GN=LOC103490261 PE=4 SV=1)

HSP 1 Score: 832.8 bits (2150), Expect = 7.7e-238
Identity = 414/509 (81.34%), Postives = 430/509 (84.48%), Query Frame = 0

Query: 1   MNKRARIGHSNKAVVNVWKREVGELSSRDFAHRLGASEDLVLRLDLFRKLERHKGCVNTV 60
           MNKRARI  SNKAVVNVWKREVGELS+R+FAHRLGA EDLVLRLDL++KLERHKGCVNTV
Sbjct: 1   MNKRARIDDSNKAVVNVWKREVGELSTRNFAHRLGALEDLVLRLDLYKKLERHKGCVNTV 60

Query: 61  SFSAAGDILVSGSDDRRVILWNWETGHAKLCFQSGHNNNVFQAKIMPYTDDNSIVTCAAD 120
           SF+AAGDILVSGSDDRRVILWNWETG  KL FQSGHNNNVFQAKIMPYTDD SIVTCAAD
Sbjct: 61  SFNAAGDILVSGSDDRRVILWNWETGRVKLSFQSGHNNNVFQAKIMPYTDDRSIVTCAAD 120

Query: 121 GQVRHAQILDGGDVETALLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRTGVAIE 180
           GQVRHAQIL+ G VET+LLGSH GRAHKLAIEPGSPY+FYTCGEDGLVQ FDLRTG A+E
Sbjct: 121 GQVRHAQILNSGHVETSLLGSHLGRAHKLAIEPGSPYMFYTCGEDGLVQRFDLRTGAAVE 180

Query: 181 LFTCQSVDKRAGYMSSIQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQP 240
           LFTCQSVD RAGYMSSIQLNAI IDPRNPNLFVVAGSDEYARLYDIRK   +GSTDFGQ 
Sbjct: 181 LFTCQSVDNRAGYMSSIQLNAIVIDPRNPNLFVVAGSDEYARLYDIRKSGEDGSTDFGQL 240

Query: 241 ADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLGRSPYP-----LSP 300
           ADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLG +P P     LS 
Sbjct: 241 ADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLGPNPNPAPPLSLSS 300

Query: 301 GTSDMGAEKVKDGSPQVYKGHRNCETVKGVNFFGPKCEYVISGSDCGRIFIWRKKGGVLI 360
             S+MGAEKV +G PQVYKGHRNCETVKGVNFFGPKCEYV+SGSDCGRIFIWRKKGG LI
Sbjct: 301 DASEMGAEKVDNGIPQVYKGHRNCETVKGVNFFGPKCEYVVSGSDCGRIFIWRKKGGELI 360

Query: 361 RVMEADEDVVNCIEPHPHMTMLASSGIESDVKLWTPKALERATLPEKIEQNIGFVMIGIV 420
           RVMEADEDVVNCIEPHPHMTMLASSGIESDVK+WTPKA ERATLPEKIEQ          
Sbjct: 361 RVMEADEDVVNCIEPHPHMTMLASSGIESDVKIWTPKAHERATLPEKIEQ---------- 420

Query: 421 FNDDYKDDEDNEGNDDKGKKLKPRGWMHRLALPEELMMQLFSLRRQNSSPEHRRENSAVG 480
                              K KPRGWMHRLALPEELMMQLFSLRRQ SSPE  RE   V 
Sbjct: 421 -------------------KPKPRGWMHRLALPEELMMQLFSLRRQTSSPERGREPPTVS 480

Query: 481 REILELIMTFNNNTESSSEDNGGDTAEDD 505
           RE+LELIMTFNN     S D   DT EDD
Sbjct: 481 RELLELIMTFNNGNSDDSSDYNDDTGEDD 480

BLAST of Spg014981 vs. ExPASy TrEMBL
Match: A0A5D3DIH7 (DDB1-and CUL4-associated factor 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G004590 PE=4 SV=1)

HSP 1 Score: 827.4 bits (2136), Expect = 3.2e-236
Identity = 412/509 (80.94%), Postives = 428/509 (84.09%), Query Frame = 0

Query: 1   MNKRARIGHSNKAVVNVWKREVGELSSRDFAHRLGASEDLVLRLDLFRKLERHKGCVNTV 60
           MNKRARI  SNKAVVNVWKREVGELS+R+FAHRLGA EDLVLRLDL++KLERHKGCVNTV
Sbjct: 1   MNKRARIDDSNKAVVNVWKREVGELSTRNFAHRLGALEDLVLRLDLYKKLERHKGCVNTV 60

Query: 61  SFSAAGDILVSGSDDRRVILWNWETGHAKLCFQSGHNNNVFQAKIMPYTDDNSIVTCAAD 120
           SF+AAGDILVSGSDDRRVILWNWETG  KL FQSGHNNNVFQAKIMPYTDD SIVTCAAD
Sbjct: 61  SFNAAGDILVSGSDDRRVILWNWETGRVKLSFQSGHNNNVFQAKIMPYTDDRSIVTCAAD 120

Query: 121 GQVRHAQILDGGDVETALLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRTGVAIE 180
           GQVRHAQIL+ G VET+LLGSH GRAHKLAIEPGSPY+FYTCGEDGLVQ FDLRTG A+E
Sbjct: 121 GQVRHAQILNSGHVETSLLGSHLGRAHKLAIEPGSPYMFYTCGEDGLVQRFDLRTGAAVE 180

Query: 181 LFTCQSVDKRAGYMSSIQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQP 240
           LFTCQSVD RAGYMSSIQLNAI IDPRNPNLFVVAGSDEYARLYDIRK   +GSTDFGQ 
Sbjct: 181 LFTCQSVDNRAGYMSSIQLNAIVIDPRNPNLFVVAGSDEYARLYDIRKSGEDGSTDFGQL 240

Query: 241 ADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLGRSPYP-----LSP 300
            D YCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLG +P P     LS 
Sbjct: 241 VDSYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLGPNPNPAPPLSLSS 300

Query: 301 GTSDMGAEKVKDGSPQVYKGHRNCETVKGVNFFGPKCEYVISGSDCGRIFIWRKKGGVLI 360
             S+MGAEKV +G PQVYKGHRNCETVKGVNFFGPKCEYV+SGSDCGRIFIWRKKGG LI
Sbjct: 301 DASEMGAEKVDNGIPQVYKGHRNCETVKGVNFFGPKCEYVVSGSDCGRIFIWRKKGGELI 360

Query: 361 RVMEADEDVVNCIEPHPHMTMLASSGIESDVKLWTPKALERATLPEKIEQNIGFVMIGIV 420
           RVMEADEDVVNCIEPHPHMTMLASSGIESDVK+WTPKA ERATLPEKIEQ          
Sbjct: 361 RVMEADEDVVNCIEPHPHMTMLASSGIESDVKIWTPKAHERATLPEKIEQ---------- 420

Query: 421 FNDDYKDDEDNEGNDDKGKKLKPRGWMHRLALPEELMMQLFSLRRQNSSPEHRRENSAVG 480
                              K KPRGWMHRLALPEELMMQLFSLRRQ SSPE  RE   V 
Sbjct: 421 -------------------KPKPRGWMHRLALPEELMMQLFSLRRQTSSPERGREPPTVS 480

Query: 481 REILELIMTFNNNTESSSEDNGGDTAEDD 505
           RE+LELIMTFNN     S D   DT EDD
Sbjct: 481 RELLELIMTFNNGNSDDSSDYNDDTGEDD 480

BLAST of Spg014981 vs. ExPASy TrEMBL
Match: A0A0A0K4R4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G016620 PE=4 SV=1)

HSP 1 Score: 823.9 bits (2127), Expect = 3.6e-235
Identity = 410/509 (80.55%), Postives = 428/509 (84.09%), Query Frame = 0

Query: 1   MNKRARIGHSNKAVVNVWKREVGELSSRDFAHRLGASEDLVLRLDLFRKLERHKGCVNTV 60
           MNKRARI  SNKAVVNVWKREVGELS+R+FAHRLGA EDLVLRLDL++KLERHKGCVNTV
Sbjct: 1   MNKRARIDDSNKAVVNVWKREVGELSTRNFAHRLGALEDLVLRLDLYKKLERHKGCVNTV 60

Query: 61  SFSAAGDILVSGSDDRRVILWNWETGHAKLCFQSGHNNNVFQAKIMPYTDDNSIVTCAAD 120
           SF+AAGDILVSGSDDRRVILWNWETG  KL FQSGHNNNVFQAKIMPYTDD SIVTCAAD
Sbjct: 61  SFNAAGDILVSGSDDRRVILWNWETGRVKLSFQSGHNNNVFQAKIMPYTDDQSIVTCAAD 120

Query: 121 GQVRHAQILDGGDVETALLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRTGVAIE 180
           GQVRHAQIL+ G VET+LLG+H GRAHKLAIEPGSPY+FYTCGEDGLVQ FDLRTG A+E
Sbjct: 121 GQVRHAQILNSGHVETSLLGNHLGRAHKLAIEPGSPYMFYTCGEDGLVQRFDLRTGDAVE 180

Query: 181 LFTCQSVDKRAGYMSSIQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQP 240
           LFTCQSVD RAGYMSSIQLNAI IDPRNPNLFVVAGSDEYARLYDIRK   +GSTDFGQ 
Sbjct: 181 LFTCQSVDNRAGYMSSIQLNAIVIDPRNPNLFVVAGSDEYARLYDIRKSGEDGSTDFGQL 240

Query: 241 ADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLGRSPYP-----LSP 300
           ADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLG +P P     LS 
Sbjct: 241 ADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLGPNPNPVPPLSLSS 300

Query: 301 GTSDMGAEKVKDGSPQVYKGHRNCETVKGVNFFGPKCEYVISGSDCGRIFIWRKKGGVLI 360
             S+MGAE V +G PQVYKGHRNCETVKGVNFFGPKCEYV+SGSDCGRIFIWRKKGG LI
Sbjct: 301 DASEMGAENVDNGIPQVYKGHRNCETVKGVNFFGPKCEYVVSGSDCGRIFIWRKKGGDLI 360

Query: 361 RVMEADEDVVNCIEPHPHMTMLASSGIESDVKLWTPKALERATLPEKIEQNIGFVMIGIV 420
           RVMEADEDVVNCIEPHPHMTMLASSGIESDVK+WTPKA ERATLPEKIEQ          
Sbjct: 361 RVMEADEDVVNCIEPHPHMTMLASSGIESDVKIWTPKAHERATLPEKIEQ---------- 420

Query: 421 FNDDYKDDEDNEGNDDKGKKLKPRGWMHRLALPEELMMQLFSLRRQNSSPEHRRENSAVG 480
                              K KPRGWMHRLALPEELMMQLFSLRRQ SSPE  RE   V 
Sbjct: 421 -------------------KPKPRGWMHRLALPEELMMQLFSLRRQTSSPESGREPPTVS 480

Query: 481 REILELIMTFNNNTESSSEDNGGDTAEDD 505
           RE+LELI TFNN     S D   D+ EDD
Sbjct: 481 RELLELIRTFNNGNSDDSSDYNDDSGEDD 480

BLAST of Spg014981 vs. ExPASy TrEMBL
Match: A0A6J1HVC3 (DDB1- and CUL4-associated factor 8-like OS=Cucurbita maxima OX=3661 GN=LOC111468181 PE=4 SV=1)

HSP 1 Score: 788.5 bits (2035), Expect = 1.7e-224
Identity = 395/512 (77.15%), Postives = 428/512 (83.59%), Query Frame = 0

Query: 1   MNKRARIGHSNKAVVNVWKREVGELSSRDFAHRLGASEDLVLRLDLFRKLERHKGCVNTV 60
           MNKRAR   S+KAVVN  KREVGE+S+R+FA+RLGA EDLVLRL L RKLERHKGCVNTV
Sbjct: 1   MNKRARFDDSSKAVVNFLKREVGEISTRNFANRLGALEDLVLRLGLLRKLERHKGCVNTV 60

Query: 61  SFSAAGDILVSGSDDRRVILWNWETGHAKLCFQSGHNNNVFQAKIMPYTDDNSIVTCAAD 120
           SF+AAGD+LVSGSDDRRVILWNWE G  ++ FQSGHNNNVFQAK MPYTDD SIVTCAAD
Sbjct: 61  SFNAAGDVLVSGSDDRRVILWNWEAGQVEISFQSGHNNNVFQAKFMPYTDDRSIVTCAAD 120

Query: 121 GQVRHAQILDGGDVETALLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRTGVAIE 180
           GQVRHAQ+L+GG VET+LLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQ FDLRTG A+E
Sbjct: 121 GQVRHAQVLNGGRVETSLLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQRFDLRTGAAVE 180

Query: 181 LFTCQSVDKRAGYMSSIQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKYKGEG--STDFG 240
           LFTCQSV  RAGYMS++QLNAIA+DPRNPNL VVAGSDEYARLYDIRK  GEG  STD G
Sbjct: 181 LFTCQSVGNRAGYMSTVQLNAIAMDPRNPNLVVVAGSDEYARLYDIRKCGGEGSTSTDSG 240

Query: 241 QPADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLGRSPYPLSP--- 300
           +PADCYCPPHLI DEQVGITGLAFSELSELLVSYNDE IYLFNRDMGLG +P  +SP   
Sbjct: 241 KPADCYCPPHLIADEQVGITGLAFSELSELLVSYNDEYIYLFNRDMGLGPNPNAVSPVSL 300

Query: 301 --GTSDMGAEKVKDGSPQVYKGHRNCETVKGVNFFGPKCEYVISGSDCGRIFIWRKKGGV 360
              TS+MGAEKV + +PQVYKGHRNCETVKGVNFFGPKCEYVISGSDCGRIFIWRKKGG 
Sbjct: 301 SSDTSEMGAEKVVNCTPQVYKGHRNCETVKGVNFFGPKCEYVISGSDCGRIFIWRKKGGE 360

Query: 361 LIRVMEADEDVVNCIEPHPHMTMLASSGIESDVKLWTPKALERATLPEKIEQNIGFVMIG 420
           LIRVM+ADEDVVNCIEPHPHMTMLASSGIESDVK+WTP +  RATLPEKIEQN       
Sbjct: 361 LIRVMKADEDVVNCIEPHPHMTMLASSGIESDVKIWTPNSRHRATLPEKIEQN------- 420

Query: 421 IVFNDDYKDDEDNEGNDDKGKKLKPRGWMHRLALPEELMMQLFSL-RRQNSSPEHRRENS 480
                                  KPRGWMHRLALPEE+MMQLFSL RR+NS+P   RENS
Sbjct: 421 ----------------------PKPRGWMHRLALPEEVMMQLFSLRRRRNSNPVRGRENS 480

Query: 481 AVGREILELIMTF-NNNTESSSEDNGGDTAED 504
              R+ILELIMTF N+N++ SS+ N GD AED
Sbjct: 481 TPNRDILELIMTFSNSNSDGSSDYNAGDAAED 483

BLAST of Spg014981 vs. TAIR 10
Match: AT4G35140.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 642.1 bits (1655), Expect = 3.7e-184
Identity = 320/521 (61.42%), Postives = 390/521 (74.86%), Query Frame = 0

Query: 1   MNKRARIGHSN-----KAVVNVWKREVGELSSRDFAHRLGASEDLVLRLDLFRKLERHKG 60
           M+++AR G SN       VV+ W+REVG +SSR+F++R  ASE+LVLRL++++KLE+HKG
Sbjct: 1   MSEKARRGRSNFNGLHHPVVDFWRREVGGISSRNFSNRFSASENLVLRLEIYKKLEKHKG 60

Query: 61  CVNTVSFSAAGDILVSGSDDRRVILWNWETGHAKLCFQSGHNNNVFQAKIMPYTDDNSIV 120
           CVNTVSF+A GD+L+SGSDDRRV+LW+W+ G+ KL F SGH NNVFQAK MP++DD +IV
Sbjct: 61  CVNTVSFNAEGDVLISGSDDRRVVLWDWQLGNVKLSFHSGHANNVFQAKFMPFSDDRTIV 120

Query: 121 TCAADGQVRHAQILDGGDVETALLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRT 180
           TCAADG VR A IL+G  VET+ LG HQGRAHKL IEPG+P+IFYTCGEDGLVQ FDLRT
Sbjct: 121 TCAADGMVRRASILEGDKVETSFLGLHQGRAHKLCIEPGNPHIFYTCGEDGLVQRFDLRT 180

Query: 181 GVAIELFTCQSVDKRAGYMSSIQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKYKGEGST 240
               ELFTC+SVD R   M +IQLNAIAIDPRN NLF V G +EYARLYDIR+++GEG  
Sbjct: 181 EAPTELFTCRSVDPRRRNMDAIQLNAIAIDPRNSNLFAVGGMEEYARLYDIRRFQGEGLN 240

Query: 241 DFGQPADCYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLGRSPYPLSP 300
            F + AD +CPPHLIG+E VGITGLAFSE SELLVSYNDE IYLF   MGLG +P P SP
Sbjct: 241 GFTRAADHFCPPHLIGNEDVGITGLAFSEQSELLVSYNDEFIYLFTPGMGLGSNPIPSSP 300

Query: 301 GTSDMGAEKVKDGSPQ---------VYKGHRNCETVKGVNFFGPKCEYVISGSDCGRIFI 360
            +    ++     SP+         VYKGH+NCETVKGVNFFGP+ EYV+SGSDCGRIFI
Sbjct: 301 ISKSPVSKSESSSSPKDENEHSVSLVYKGHKNCETVKGVNFFGPRSEYVVSGSDCGRIFI 360

Query: 361 WRKKGGVLIRVMEADEDVVNCIEPHPHMTMLASSGIESDVKLWTPKALERATLPEKIEQN 420
           WRKKGG LIRVMEAD  VVNCIEPHPH+ +LASSGIESD+K+WT KA ERATLPE IE  
Sbjct: 361 WRKKGGELIRVMEADRHVVNCIEPHPHIPVLASSGIESDIKVWTSKAAERATLPENIEL- 420

Query: 421 IGFVMIGIVFNDDYKDDEDNEGNDDKGKKLKPRGWMHRLALPEELMMQLFSLR-RQNSSP 480
                                      +K  PRGWM+R++ P EL+ QLFSL+ R +SSP
Sbjct: 421 ---------------------------RKRTPRGWMYRVSSPHELLAQLFSLQNRSSSSP 480

Query: 481 EHRRE-NSAVGREILELIMTFNNNT--ESSSEDNGGDTAED 504
           E   E +SA GRE+L+LI+TFN+ +  E++++D+ G++ ED
Sbjct: 481 EREGESSSATGRELLDLILTFNDQSDDENATDDDDGNSHED 493

BLAST of Spg014981 vs. TAIR 10
Match: AT4G38480.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 543.5 bits (1399), Expect = 1.8e-154
Identity = 266/434 (61.29%), Postives = 319/434 (73.50%), Query Frame = 0

Query: 3   KRARIGHSNKAVVNVWKREVGELSSRDFAHRLGASEDLVLRLDLFRKLERHKGCVNTVSF 62
           KR R  HS   VVNVW+RE+G L +R F++R  ASEDL+ RL L +KL++HKGCVNTVSF
Sbjct: 7   KRPRTDHS---VVNVWERELGLLPNRSFSNRFSASEDLLRRLGLDKKLDKHKGCVNTVSF 66

Query: 63  SAAGDILVSGSDDRRVILWNWETGHAKLCFQSGHNNNVFQAKIMPYTDDNSIVTCAADGQ 122
           +A GDIL+SGSDDR+VILW+W+T   KL F SGH NN+FQAK MP++DD +IVT AAD Q
Sbjct: 67  NADGDILLSGSDDRQVILWDWQTASVKLSFDSGHFNNIFQAKFMPFSDDRTIVTSAADKQ 126

Query: 123 VRHAQILDGGDVETALLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRTGVAIELF 182
           VR+++IL+ G VET+LLG HQG  HKLA+EPGSP+ FYTCGEDG V+HFDLRT VA  LF
Sbjct: 127 VRYSKILESGQVETSLLGKHQGPVHKLAVEPGSPFSFYTCGEDGAVKHFDLRTRVATNLF 186

Query: 183 TCQSVDKRAGYMSSIQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQPAD 242
           TC    K A +   + L+AIA+DPRNP L  VAG DEYAR+YDIR Y+ EG  +F QP D
Sbjct: 187 TC----KEAKFNLVVYLHAIAVDPRNPGLLAVAGMDEYARVYDIRSYRSEGWYNFTQPID 246

Query: 243 CYCPPHLIGDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLGRSPYPLSPGTSDMGA 302
            +CP HLIGD+ VGITGLAFS+ SELL SY+DE IYLF  DMGLG +PYP S  T +   
Sbjct: 247 HFCPGHLIGDDHVGITGLAFSDQSELLASYSDEFIYLFTPDMGLGPTPYPSSTKTEER-- 306

Query: 303 EKVKDGSPQVYKGHRNCETVKGVNFFGPKCEYVISGSDCGRIFIWRKKGGVLIRVMEADE 362
                 +PQVYK H N ETVKGVNFFGPKCEYV+SGSDCGRIFIWRKK G L+R MEAD 
Sbjct: 307 -----MTPQVYKEHTNRETVKGVNFFGPKCEYVVSGSDCGRIFIWRKKDGELLRAMEADR 366

Query: 363 DVVNCIEPHPHMTMLASSGIESDVKLWTPKALERATLPEKIEQNIGFVMIG-----IVFN 422
            VVNCIE HPHM ++ SSGI++D+K+WTP   E+   P   +Q  GF          V +
Sbjct: 367 HVVNCIESHPHMPLMCSSGIDTDIKIWTPGGTEKPLSPGNAKQASGFGNPRWFDGYYVDD 426

Query: 423 DDYKDDEDNEGNDD 432
           DD  DDE +E + D
Sbjct: 427 DDDSDDESSEESSD 426

BLAST of Spg014981 vs. TAIR 10
Match: AT3G45620.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 424.9 bits (1091), Expect = 9.3e-119
Identity = 211/404 (52.23%), Postives = 270/404 (66.83%), Query Frame = 0

Query: 17  VWKREVGELSSRDFAHRLGASEDLVLRLDLFRKLERHKGCVNTVSFSAAGDILVSGSDDR 76
           ++ RE+G       + R+ ASE  V +LDL+ KL  H+GCVN V F++ GD+LVSGSDDR
Sbjct: 18  IFNREIGFSHPITISRRISASEGRVKKLDLYGKLNGHEGCVNAVEFNSTGDVLVSGSDDR 77

Query: 77  RVILWNWETGHAKLCFQSGHNNNVFQAKIMPYTDDNSIVTCAADGQVRHAQILDGGDVET 136
           +++LWNW +G  KL + SGH  NVFQ K +P+TDD +I+T  ADGQVR  QIL+ G VET
Sbjct: 78  QIMLWNWLSGSRKLSYPSGHCENVFQTKFIPFTDDRTIITSGADGQVRLGQILENGKVET 137

Query: 137 ALLGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLRTGVA-IELFTCQSVD--KRAGY 196
             LG H GR +KLA+ PG P +FY+CGEDG VQHFD+R+  A + L++       +R   
Sbjct: 138 KRLGRHHGRVYKLAVLPGDPNVFYSCGEDGFVQHFDIRSNSATMVLYSSPFTQGCRRHHS 197

Query: 197 MSSIQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDF---GQPADCYCPPHLI 256
            S I+LN+IAIDPRN     V GSDEYAR+YD R+ +            P + +CP HL 
Sbjct: 198 SSRIRLNSIAIDPRNSYYLAVGGSDEYARVYDTRRVQLAPVCRHVLPDAPVNTFCPRHLR 257

Query: 257 GDEQVGITGLAFSELSELLVSYNDESIYLFNRDMGLGRSPYPLSPGTSDMGAEKVKD-GS 316
               V ITGLA+S+  ELLVSYNDE IYLF ++MG G SP  +SP       EK+++   
Sbjct: 258 ETNSVHITGLAYSKAGELLVSYNDELIYLFEKNMGYGSSPVSVSP-------EKLQEMEE 317

Query: 317 PQVYKGHRNCETVKGVNFFGPKCEYVISGSDCGRIFIWRKKGGVLIRVMEADEDVVNCIE 376
           PQVY GHRN +TVKGVNFFGP  EYV SGSDCG IFIW+KKGG L+R M  D  VVN +E
Sbjct: 318 PQVYIGHRNAQTVKGVNFFGPNDEYVTSGSDCGHIFIWKKKGGKLVRAMVGDRRVVNQLE 377

Query: 377 PHPHMTMLASSGIESDVKLWTPKALERATLPEKIEQNIGFVMIG 414
            HPH+ +LAS GIE  VKLWTP + +  +LPEKI++ +    +G
Sbjct: 378 SHPHIPLLASCGIEKSVKLWTPMSNDVLSLPEKIDKVMELNRVG 414

BLAST of Spg014981 vs. TAIR 10
Match: AT3G45620.2 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 412.5 bits (1059), Expect = 4.8e-115
Identity = 204/380 (53.68%), Postives = 258/380 (67.89%), Query Frame = 0

Query: 41  VLRLDLFRKLERHKGCVNTVSFSAAGDILVSGSDDRRVILWNWETGHAKLCFQSGHNNNV 100
           V +LDL+ KL  H+GCVN V F++ GD+LVSGSDDR+++LWNW +G  KL + SGH  NV
Sbjct: 76  VKKLDLYGKLNGHEGCVNAVEFNSTGDVLVSGSDDRQIMLWNWLSGSRKLSYPSGHCENV 135

Query: 101 FQAKIMPYTDDNSIVTCAADGQVRHAQILDGGDVETALLGSHQGRAHKLAIEPGSPYIFY 160
           FQ K +P+TDD +I+T  ADGQVR  QIL+ G VET  LG H GR +KLA+ PG P +FY
Sbjct: 136 FQTKFIPFTDDRTIITSGADGQVRLGQILENGKVETKRLGRHHGRVYKLAVLPGDPNVFY 195

Query: 161 TCGEDGLVQHFDLRTGVA-IELFTCQSVD--KRAGYMSSIQLNAIAIDPRNPNLFVVAGS 220
           +CGEDG VQHFD+R+  A + L++       +R    S I+LN+IAIDPRN     V GS
Sbjct: 196 SCGEDGFVQHFDIRSNSATMVLYSSPFTQGCRRHHSSSRIRLNSIAIDPRNSYYLAVGGS 255

Query: 221 DEYARLYDIRKYKGEGSTDF---GQPADCYCPPHLIGDEQVGITGLAFSELSELLVSYND 280
           DEYAR+YD R+ +            P + +CP HL     V ITGLA+S+  ELLVSYND
Sbjct: 256 DEYARVYDTRRVQLAPVCRHVLPDAPVNTFCPRHLRETNSVHITGLAYSKAGELLVSYND 315

Query: 281 ESIYLFNRDMGLGRSPYPLSPGTSDMGAEKVKD-GSPQVYKGHRNCETVKGVNFFGPKCE 340
           E IYLF ++MG G SP  +SP       EK+++   PQVY GHRN +TVKGVNFFGP  E
Sbjct: 316 ELIYLFEKNMGYGSSPVSVSP-------EKLQEMEEPQVYIGHRNAQTVKGVNFFGPNDE 375

Query: 341 YVISGSDCGRIFIWRKKGGVLIRVMEADEDVVNCIEPHPHMTMLASSGIESDVKLWTPKA 400
           YV SGSDCG IFIW+KKGG L+R M  D  VVN +E HPH+ +LAS GIE  VKLWTP +
Sbjct: 376 YVTSGSDCGHIFIWKKKGGKLVRAMVGDRRVVNQLESHPHIPLLASCGIEKSVKLWTPMS 435

Query: 401 LERATLPEKIEQNIGFVMIG 414
            +  +LPEKI++ +    +G
Sbjct: 436 NDVLSLPEKIDKVMELNRVG 448

BLAST of Spg014981 vs. TAIR 10
Match: AT5G10940.2 (transducin family protein / WD-40 repeat family protein )

HSP 1 Score: 153.3 bits (386), Expect = 5.2e-37
Identity = 99/305 (32.46%), Postives = 151/305 (49.51%), Query Frame = 0

Query: 27  SRDFAHRLGASEDLVLRLDLFRKLERHKGCVNTVSFSAAGDILVSGSDDRRVILWNWETG 86
           S +   R+     LV RL   ++LE H+GCVN +++++ G +L+SGSDD R+ +WN+ + 
Sbjct: 23  SHEVDQRMQFHSSLVRRLSQEQELEGHQGCVNALAWNSNGSLLISGSDDLRINIWNYSSR 82

Query: 87  HAKLCFQSGHNNNVFQAKIMPYTDDNSIVTCAADGQVRHAQIL--------DGGDVETAL 146
                  +GH  N+F  K +P T D  +V+ A D +VR             D   + +AL
Sbjct: 83  KLLHSIDTGHTANIFCTKFVPETSDELVVSGAGDAEVRLFNTSRLSGRAEDDNAIIPSAL 142

Query: 147 LGSHQGRAHKLAIEPGSPYIFYTCGEDGLVQHFDLR-------TGVAIELFTCQSV--DK 206
              H  R  KLA+EPG+P + ++  EDG ++  D R        G A +   C+SV  D 
Sbjct: 143 YQCHTRRVKKLAVEPGNPNVVWSASEDGTLRQHDFRESTSCPPAGTAHQ--ECRSVLLDL 202

Query: 207 RAGY--------MSSIQLNAIAIDPRNPNLFVVAGSDEYARLYDIRKYKGEGSTDFGQP- 266
           R+G           ++ L +  I    P+L +V GSD +ARLYD R      S+    P 
Sbjct: 203 RSGAKRALADPPKQTLSLKSCDISATRPHLLLVGGSDAFARLYDRRMLPPLASSRKRMPP 262

Query: 267 ---ADCYCPPHLI--GDEQVGITGLAFS-ELSELLVSYNDESIYLFNRDMGLGRSPYPLS 300
               + +CP HL   G   + +T + FS    E+L+SY+ E +YL N + G G   Y  +
Sbjct: 263 PPCVNYFCPMHLSERGRTNLHLTHVTFSPNGEEVLLSYSGEHVYLMNVNNGTGIMQY--T 322


HSP 2 Score: 88.6 bits (218), Expect = 1.6e-17
Identity = 39/82 (47.56%), Postives = 58/82 (70.73%), Query Frame = 0

Query: 313 YKGHRNCET-VKGVNFFGPKCEYVISGSDCGRIFIWRKKGGVLIRVMEADEDVVNCIEPH 372
           Y GH N  T +K  +F G + EY+ SGSD GR FIW K+ G L++V+  DE V+NCI+ H
Sbjct: 610 YVGHCNVGTDIKQASFLGQRGEYIASGSDDGRWFIWEKQTGRLMKVLVGDESVLNCIQCH 669

Query: 373 PHMTMLASSGIESDVKLWTPKA 394
           P  +++A+SGI++ +K+W+P A
Sbjct: 670 PFDSVVATSGIDNTIKIWSPTA 691

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022136068.16.4e-24782.71DDB1- and CUL4-associated factor 8 [Momordica charantia][more]
XP_038887987.11.2e-23781.76DDB1- and CUL4-associated factor 8-like [Benincasa hispida][more]
XP_008447917.11.6e-23781.34PREDICTED: DDB1- and CUL4-associated factor 8 [Cucumis melo][more]
TYK23393.16.7e-23680.94DDB1- and CUL4-associated factor 8 [Cucumis melo var. makuwa][more]
XP_004144864.17.4e-23580.55DDB1- and CUL4-associated factor 8 [Cucumis sativus] >KGN43277.1 hypothetical pr... [more]
Match NameE-valueIdentityDescription
Q8N7N55.4e-7942.17DDB1- and CUL4-associated factor 8 OS=Mus musculus OX=10090 GN=Dcaf8 PE=1 SV=1[more]
Q5TAQ92.7e-7841.92DDB1- and CUL4-associated factor 8 OS=Homo sapiens OX=9606 GN=DCAF8 PE=1 SV=1[more]
Q5U2M63.5e-7841.67DDB1- and CUL4-associated factor 8 OS=Rattus norvegicus OX=10116 GN=Dcaf8 PE=1 S... [more]
Q5R4482.9e-7741.67DDB1- and CUL4-associated factor 8 OS=Pongo abelii OX=9601 GN=DCAF8 PE=2 SV=1[more]
Q6NRH13.8e-7740.71DDB1- and CUL4-associated factor 8 OS=Xenopus laevis OX=8355 GN=dcaf8 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1C6L23.1e-24782.71DDB1- and CUL4-associated factor 8 OS=Momordica charantia OX=3673 GN=LOC11100785... [more]
A0A1S3BIJ47.7e-23881.34DDB1- and CUL4-associated factor 8 OS=Cucumis melo OX=3656 GN=LOC103490261 PE=4 ... [more]
A0A5D3DIH73.2e-23680.94DDB1-and CUL4-associated factor 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A0A0K4R43.6e-23580.55Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G016620 PE=4 SV=1[more]
A0A6J1HVC31.7e-22477.15DDB1- and CUL4-associated factor 8-like OS=Cucurbita maxima OX=3661 GN=LOC111468... [more]
Match NameE-valueIdentityDescription
AT4G35140.13.7e-18461.42Transducin/WD40 repeat-like superfamily protein [more]
AT4G38480.11.8e-15461.29Transducin/WD40 repeat-like superfamily protein [more]
AT3G45620.19.3e-11952.23Transducin/WD40 repeat-like superfamily protein [more]
AT3G45620.24.8e-11553.68Transducin/WD40 repeat-like superfamily protein [more]
AT5G10940.25.2e-3732.46transducin family protein / WD-40 repeat family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001680WD40 repeatSMARTSM00320WD40_4coord: 85..127
e-value: 15.0
score: 9.5
coord: 132..172
e-value: 1.6
score: 15.8
coord: 183..225
e-value: 11.0
score: 10.4
coord: 306..348
e-value: 1.4
score: 16.0
coord: 242..281
e-value: 81.0
score: 5.0
coord: 43..82
e-value: 1.6E-6
score: 37.7
coord: 351..390
e-value: 0.028
score: 23.6
IPR001680WD40 repeatPFAMPF00400WD40coord: 47..82
e-value: 3.1E-5
score: 24.6
coord: 311..347
e-value: 0.17
score: 12.8
IPR001680WD40 repeatPROSITEPS50082WD_REPEATS_2coord: 50..82
score: 13.248743
IPR015943WD40/YVTN repeat-like-containing domain superfamilyGENE3D2.130.10.10coord: 39..392
e-value: 8.2E-49
score: 168.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 484..504
NoneNo IPR availablePANTHERPTHR15574:SF62WD40/YVTN REPEAT-LIKE-CONTAINING DOMAIN-CONTAINING PROTEIN-RELATEDcoord: 8..405
NoneNo IPR availablePROSITEPS50294WD_REPEATS_REGIONcoord: 50..82
score: 10.996502
IPR045151DDB1- and CUL4-associated factor 8-likePANTHERPTHR15574WD REPEAT DOMAIN-CONTAINING FAMILYcoord: 8..405
IPR036322WD40-repeat-containing domain superfamilySUPERFAMILY50978WD40 repeat-likecoord: 42..389

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg014981.1Spg014981.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0080008 Cul4-RING E3 ubiquitin ligase complex
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005515 protein binding