Spg014889 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg014889 vs. NCBI nr
Match: XP_038897980.1 (subtilisin-like protease SBT1.4 [Benincasa hispida])

HSP 1 Score: 1406.0 bits (3638), Expect = 0.0e+00
Identity = 697/772 (90.28%), Postives = 735/772 (95.21%), Query Frame = 0

Query: 1   MASFHLCSSVFLFFFILCFVPVIFSRSPEDQQTFIVHVSKSEKPSLFSSHHHWHSSILES 60
           MA  HLCSS+F F FILCF PVIFSRSPE+Q+TFIVHVSKSEKPSLFSSHHHWHSSILES
Sbjct: 1   MAKSHLCSSLF-FLFILCFAPVIFSRSPENQETFIVHVSKSEKPSLFSSHHHWHSSILES 60

Query: 61  LSPSPHPTKLLYNYERAANGFSAHITAAQAAELRQVPGVISVIPDRIRQLHTTRTPHFLG 120
           LSPSPHPTKLLYNYERA NGFSA +TAAQAA+LR VPGVISVIPDRIRQLHTTRTPHFLG
Sbjct: 61  LSPSPHPTKLLYNYERAVNGFSARLTAAQAAQLRHVPGVISVIPDRIRQLHTTRTPHFLG 120

Query: 121 LADNLGLWPDANYAEDVIIGVLDTGIWPERPSFSDEGLSPVPESWKGICETGDRFSASAC 180
           LADNLGLWPD NYA+DVIIGVLDTGIWPERPSFSDEGLSPVP SWKG CETG+ FSAS C
Sbjct: 121 LADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASFC 180

Query: 181 NRKIIGARAYFYGYESNLGRSLQDSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240
           NRKIIGARAYFYGY SN GRS   SSDFKSARDT+GHGTHTASTAAGSFVKNASFFQYAR
Sbjct: 181 NRKIIGARAYFYGYASNFGRSFNGSSDFKSARDTEGHGTHTASTAAGSFVKNASFFQYAR 240

Query: 241 GEARGMASKARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSTGRAPAYYRDS 300
           GEARGMAS+ARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGS+GRAPAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 300

Query: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360
           IAIGAFGAMQHG+VVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF
Sbjct: 301 IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360

Query: 361 SGVSLYSGDPLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSSV 420
           SGVSLYSGDPLGDSK+PLVY GDCG RYCYSG+L+ SKVAGKIVVCDRGGNARVAKG +V
Sbjct: 361 SGVSLYSGDPLGDSKVPLVYGGDCGSRYCYSGSLNPSKVAGKIVVCDRGGNARVAKGGAV 420

Query: 421 KTAGGLGMILANTEENGEELLADSHLIPGTMVGEIAGNKLKEYIHSDPNPTATIVFRGTV 480
           K+AGGLGM+LANTEENGEELLADSHLIPGTMVGEIAGNKL++YIHSDPNPTA IVFRGTV
Sbjct: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRDYIHSDPNPTAIIVFRGTV 480

Query: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFN 540
           IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILA WSGYSSPT L+IDPRRVEFN
Sbjct: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILASWSGYSSPTGLNIDPRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDTSGSPIKDLSTSKESNP 600
           IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSAL+TTSYSLD+SGSPIKDLSTS+ESNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSEESNP 600

Query: 601 FVHGAGHIDPNIALNPGLIYDINPQDYVSFLCSIGYDSKQIAVFVKESSYAKLCEHKLSN 660
           FVHGAGHIDPN ALNPGLIYDI+PQDYVSFLCSIGYDSKQIAVFVK+SSY++LCEHKLSN
Sbjct: 601 FVHGAGHIDPNKALNPGLIYDISPQDYVSFLCSIGYDSKQIAVFVKDSSYSQLCEHKLSN 660

Query: 661 PGNLNYPSFAVVFDSDAGEVVKYTRTVTNVGDEADAVYEVKVEAPEGVEISVLPKKLEFN 720
           PGNLNYPSFAVVF+ +  +VVKYTRTVTNVGDE+D VYEVKVEAP+GVEISV+P KL FN
Sbjct: 661 PGNLNYPSFAVVFNGE--DVVKYTRTVTNVGDESDVVYEVKVEAPQGVEISVVPNKLSFN 720

Query: 721 ANKTTQSYEISFTKISGFTKSASFGSIQWSDGSHIVRSPIAVSFNSGYVASM 773
           A KTT SYEI+FTKISGF ++ASFGSIQWSDGSH VRSPIAVSFN+G +ASM
Sbjct: 721 AEKTTDSYEITFTKISGFVEAASFGSIQWSDGSHNVRSPIAVSFNTGSIASM 769

BLAST of Spg014889 vs. NCBI nr
Match: XP_022964269.1 (subtilisin-like protease SBT1.4 [Cucurbita moschata])

HSP 1 Score: 1403.3 bits (3631), Expect = 0.0e+00
Identity = 698/772 (90.41%), Postives = 737/772 (95.47%), Query Frame = 0

Query: 1   MASFHLCSSVFLFFFILCFVPVIFSRSPEDQQTFIVHVSKSEKPSLFSSHHHWHSSILES 60
           MA+ HLCSS F FF ILCFVPVIFSRS ++Q+TFIVHVSKSEKP+LFSSHHHWHSSILES
Sbjct: 1   MANLHLCSSGF-FFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILES 60

Query: 61  LSPSPHPTKLLYNYERAANGFSAHITAAQAAELRQVPGVISVIPDRIRQLHTTRTPHFLG 120
           LSPSPHPTKLLY+YE AANGFSA ITAAQAAELR++PGVISVIPDR+RQLHTTRTPHFLG
Sbjct: 61  LSPSPHPTKLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLG 120

Query: 121 LADNLGLWPDANYAEDVIIGVLDTGIWPERPSFSDEGLSPVPESWKGICETGDRFSASAC 180
           LAD+ GLW + NYA+DVIIGVLDTGIWPERPSFSDEGLSPVP +WKG C+TG  FSASAC
Sbjct: 121 LADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASAC 180

Query: 181 NRKIIGARAYFYGYESNLGRSLQDSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240
           NRKIIGARAYF GYES + RSLQ SSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR
Sbjct: 181 NRKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240

Query: 241 GEARGMASKARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSTGRAPAYYRDS 300
           GEARGMAS+ARIAAYKICWE GC+DSDILAAMDQAIADGVHIISLSVGS+GR PAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDS 300

Query: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360
           IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWI+TVGASTIDREFLADVILGDGRVF
Sbjct: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVF 360

Query: 361 SGVSLYSGDPLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSSV 420
           SGVSLYSGDPLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGS+V
Sbjct: 361 SGVSLYSGDPLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAV 420

Query: 421 KTAGGLGMILANTEENGEELLADSHLIPGTMVGEIAGNKLKEYIHSDPNPTATIVFRGTV 480
           KTAGGLGMILANTEENGEELLADSHLIPGTM+GEIAGN LK+YIHSDP+PTATIVFRGTV
Sbjct: 421 KTAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTV 480

Query: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFN 540
           IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFN
Sbjct: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDTSGSPIKDLSTSKESNP 600
           IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLD+SG PI DLSTSK+SNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNP 600

Query: 601 FVHGAGHIDPNIALNPGLIYDINPQDYVSFLCSIGYDSKQIAVFVKESSYAKLCEHKLSN 660
           FVHGAGHIDPN ALNPGLIYD+NPQDYVSFLCSIGYDS+QIAVFVK+S YA+LCEHKL++
Sbjct: 601 FVHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAQLCEHKLTS 660

Query: 661 PGNLNYPSFAVVFDSDAGEVVKYTRTVTNVGDEADAVYEVKVEAPEGVEISVLPKKLEFN 720
           PGNLNYPSFAVVFDSD  EVVKYTRTVTNVGDE DAVYEVKVE+P+GVEI+VLP KLEF+
Sbjct: 661 PGNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPQGVEITVLPNKLEFS 720

Query: 721 ANKTTQSYEISFTKISGFTKSASFGSIQWSDGSHIVRSPIAVSFNSGYVASM 773
           A   TQSYEI+FTKI+G   SASFGSIQWSDGSHIVRSP+AVSFNSGY+ASM
Sbjct: 721 AKDRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM 771

BLAST of Spg014889 vs. NCBI nr
Match: KAG6593557.1 (Subtilisin-like protease 1.4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1402.9 bits (3630), Expect = 0.0e+00
Identity = 698/772 (90.41%), Postives = 737/772 (95.47%), Query Frame = 0

Query: 1   MASFHLCSSVFLFFFILCFVPVIFSRSPEDQQTFIVHVSKSEKPSLFSSHHHWHSSILES 60
           MA+  LCSS F FF ILCFVPVIFSRS ++Q+TFIVHVSKSEKP+LFSSHHHWHSSILES
Sbjct: 1   MANLRLCSSGF-FFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILES 60

Query: 61  LSPSPHPTKLLYNYERAANGFSAHITAAQAAELRQVPGVISVIPDRIRQLHTTRTPHFLG 120
           LSPSPHPTKLLY+YE AANGFSA ITAAQAAELR++PGVISVIPDR+RQLHTTRTPHFLG
Sbjct: 61  LSPSPHPTKLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLG 120

Query: 121 LADNLGLWPDANYAEDVIIGVLDTGIWPERPSFSDEGLSPVPESWKGICETGDRFSASAC 180
           LAD+ GLW + NYA+DVIIGVLDTGIWPERPSFSDEGLSPVP +WKG C+TG  FSASAC
Sbjct: 121 LADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASAC 180

Query: 181 NRKIIGARAYFYGYESNLGRSLQDSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240
           NRKIIGARAYF GYES + RSLQ SSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR
Sbjct: 181 NRKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240

Query: 241 GEARGMASKARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSTGRAPAYYRDS 300
           GEARGMAS+ARIAAYKICWEFGC+DSDILAAMDQAIADGVHIISLSVGS+GR PAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDS 300

Query: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360
           IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWI+TVGASTIDREFLADVILGDGRVF
Sbjct: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVF 360

Query: 361 SGVSLYSGDPLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSSV 420
           SGVSLYSGDPLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGS+V
Sbjct: 361 SGVSLYSGDPLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAV 420

Query: 421 KTAGGLGMILANTEENGEELLADSHLIPGTMVGEIAGNKLKEYIHSDPNPTATIVFRGTV 480
           KTAGGLGMILANTEENGEELLADSHLIPGTM+GEIAGN LK+YIHSDP+PTATIVFRGTV
Sbjct: 421 KTAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTV 480

Query: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFN 540
           IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFN
Sbjct: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDTSGSPIKDLSTSKESNP 600
           IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLD+SG PI DLSTSK+SNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNP 600

Query: 601 FVHGAGHIDPNIALNPGLIYDINPQDYVSFLCSIGYDSKQIAVFVKESSYAKLCEHKLSN 660
           FVHGAGHIDPN ALNPGLIYD+NPQDYVSFLCSIGYDS+QIAVFVK+S YA+LCEHKL++
Sbjct: 601 FVHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAQLCEHKLTS 660

Query: 661 PGNLNYPSFAVVFDSDAGEVVKYTRTVTNVGDEADAVYEVKVEAPEGVEISVLPKKLEFN 720
           PGNLNYPSFAVVFDSD  EVVKYTRTVTNVGDE DAVYEVKVE+P+GVEI+VLP KLEF+
Sbjct: 661 PGNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPQGVEITVLPNKLEFS 720

Query: 721 ANKTTQSYEISFTKISGFTKSASFGSIQWSDGSHIVRSPIAVSFNSGYVASM 773
           A   TQSYEI+FTKI+G   SASFGSIQWSDGSHIVRSP+AVSFNSGY+ASM
Sbjct: 721 AKNLTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM 771

BLAST of Spg014889 vs. NCBI nr
Match: XP_023000019.1 (subtilisin-like protease SBT1.4 [Cucurbita maxima])

HSP 1 Score: 1402.1 bits (3628), Expect = 0.0e+00
Identity = 696/772 (90.16%), Postives = 738/772 (95.60%), Query Frame = 0

Query: 1   MASFHLCSSVFLFFFILCFVPVIFSRSPEDQQTFIVHVSKSEKPSLFSSHHHWHSSILES 60
           MA+ HLCSSVF FF ILCFVPVIFSRS ++Q+TFIVHVSKSEKP+LFSSHHHWHSSIL+S
Sbjct: 1   MANLHLCSSVF-FFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILDS 60

Query: 61  LSPSPHPTKLLYNYERAANGFSAHITAAQAAELRQVPGVISVIPDRIRQLHTTRTPHFLG 120
           LSPSPHPTKLLYNYE AANGFSA ITAAQAAELR++PGVISVIPDR+RQLHTTRTPHFLG
Sbjct: 61  LSPSPHPTKLLYNYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLG 120

Query: 121 LADNLGLWPDANYAEDVIIGVLDTGIWPERPSFSDEGLSPVPESWKGICETGDRFSASAC 180
           LAD+ GLW + NYA+DVIIGVLDTGIWPERPSFSDEGLSPVP +WKG C+TG  FSASAC
Sbjct: 121 LADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASAC 180

Query: 181 NRKIIGARAYFYGYESNLGRSLQDSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240
           NRKIIGARAYF GYES + RSLQ SSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR
Sbjct: 181 NRKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240

Query: 241 GEARGMASKARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSTGRAPAYYRDS 300
           GEARGMA++ARIAAYKICWE GC+DSDILAAMDQAIADGVHIISLSVGS+GR PAYYRDS
Sbjct: 241 GEARGMANRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDS 300

Query: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360
           IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWI+TVGASTIDREFLADVILGDGRVF
Sbjct: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVF 360

Query: 361 SGVSLYSGDPLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSSV 420
           SGVSLYSGDPLGDSKLPLVYAGDCG+RYCYSGTLDSSKVAGKIVVCDRGGNARVAKGS+V
Sbjct: 361 SGVSLYSGDPLGDSKLPLVYAGDCGNRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSTV 420

Query: 421 KTAGGLGMILANTEENGEELLADSHLIPGTMVGEIAGNKLKEYIHSDPNPTATIVFRGTV 480
           KTAGGLGMILANTEENGEELLADSHLIPGTM+GEIAGN LK+YIHSDP+PTATIVFRGTV
Sbjct: 421 KTAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTV 480

Query: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFN 540
           IGDSPPAPRVAAFSSRGPN+RTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFN
Sbjct: 481 IGDSPPAPRVAAFSSRGPNFRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDTSGSPIKDLSTSKESNP 600
           IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLD+SG PI DLSTSK+SNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNP 600

Query: 601 FVHGAGHIDPNIALNPGLIYDINPQDYVSFLCSIGYDSKQIAVFVKESSYAKLCEHKLSN 660
           FVHGAGHIDPN ALNPGLIYD+NPQDYVSFLCSIGYDS+QIAVFVK+S YA+LC+HKL++
Sbjct: 601 FVHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAELCKHKLTS 660

Query: 661 PGNLNYPSFAVVFDSDAGEVVKYTRTVTNVGDEADAVYEVKVEAPEGVEISVLPKKLEFN 720
           PGNLNYPSFAVVFDSD  EVVKYTRTVTNVGDE DAVYEVKVE+PEGVEI+VLP KLEF+
Sbjct: 661 PGNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPEGVEITVLPNKLEFS 720

Query: 721 ANKTTQSYEISFTKISGFTKSASFGSIQWSDGSHIVRSPIAVSFNSGYVASM 773
           A   TQSYEI+FTKI+G   SASFGSIQWSDGSHIVRSP+AVSFNSGY+ASM
Sbjct: 721 AKNRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM 771

BLAST of Spg014889 vs. NCBI nr
Match: XP_023514101.1 (subtilisin-like protease SBT1.4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1400.6 bits (3624), Expect = 0.0e+00
Identity = 697/772 (90.28%), Postives = 736/772 (95.34%), Query Frame = 0

Query: 1   MASFHLCSSVFLFFFILCFVPVIFSRSPEDQQTFIVHVSKSEKPSLFSSHHHWHSSILES 60
           MA+ HLCSS F FF ILCFVPVIFSRS ++Q+TFIVHVSKSEKP+LFSSHHHWHSSILES
Sbjct: 1   MANLHLCSSGF-FFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILES 60

Query: 61  LSPSPHPTKLLYNYERAANGFSAHITAAQAAELRQVPGVISVIPDRIRQLHTTRTPHFLG 120
           LSPSPHPTKLLY+YE AANGFSA ITAAQAAELR++PGVISVIPDR+RQLHTTRTPHFLG
Sbjct: 61  LSPSPHPTKLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLG 120

Query: 121 LADNLGLWPDANYAEDVIIGVLDTGIWPERPSFSDEGLSPVPESWKGICETGDRFSASAC 180
           LAD+ GLW + NYA+DVIIGVLDTGIWPERPSFSDEGLSPVP +WKG C+TG  FSASAC
Sbjct: 121 LADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASAC 180

Query: 181 NRKIIGARAYFYGYESNLGRSLQDSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240
           NRKIIGARAYF GYES + RSLQ SSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR
Sbjct: 181 NRKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240

Query: 241 GEARGMASKARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSTGRAPAYYRDS 300
           GEARGMAS+ARIAAYKICWE GC+DSDILAAMDQAIADGVHIISLSVGS+GR PAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDS 300

Query: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360
           IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWI+TVGASTIDREFLADVILGDGRVF
Sbjct: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVF 360

Query: 361 SGVSLYSGDPLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSSV 420
           SGVSLYSGD LGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGS+V
Sbjct: 361 SGVSLYSGDSLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAV 420

Query: 421 KTAGGLGMILANTEENGEELLADSHLIPGTMVGEIAGNKLKEYIHSDPNPTATIVFRGTV 480
           KTAGGLGMILANTEENGEELLADSHLIPGTM+GEIAGN LK+YIHSDP+PTATIVFRGTV
Sbjct: 421 KTAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTV 480

Query: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFN 540
           IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFN
Sbjct: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDTSGSPIKDLSTSKESNP 600
           IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLD+SG PI DLSTSK+SNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNP 600

Query: 601 FVHGAGHIDPNIALNPGLIYDINPQDYVSFLCSIGYDSKQIAVFVKESSYAKLCEHKLSN 660
           FVHGAGHIDPN ALNPGLIYD+NPQDYVSFLCSIGYDS+QIAVFVK+S YA+LCEHKL++
Sbjct: 601 FVHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAQLCEHKLTS 660

Query: 661 PGNLNYPSFAVVFDSDAGEVVKYTRTVTNVGDEADAVYEVKVEAPEGVEISVLPKKLEFN 720
           PGNLNYPSFAVVFDSD  EVVKYTRTVTNVGDE DAVYEVKVE+P+GVEI+VLP KLEF+
Sbjct: 661 PGNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPQGVEITVLPNKLEFS 720

Query: 721 ANKTTQSYEISFTKISGFTKSASFGSIQWSDGSHIVRSPIAVSFNSGYVASM 773
           A   TQSYEI+FTKI+G   SASFGSIQWSDGSHIVRSP+AVSFNSGY+ASM
Sbjct: 721 AKNRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM 771

BLAST of Spg014889 vs. ExPASy Swiss-Prot
Match: Q9LVJ1 (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 987.6 bits (2552), Expect = 7.7e-287
Identity = 496/778 (63.75%), Postives = 596/778 (76.61%), Query Frame = 0

Query: 1   MASFHLCSSVFLFFFILCFVPVIFSRSPEDQQTFIVHVSKSEKPSLFSSHHHWHSSILES 60
           MA   L S  F+F  +LCF     S S +  +++IVHV +S KPSLFSSH++WH S+L S
Sbjct: 1   MAKLSLSSIFFVFPLLLCFFSP-SSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRS 60

Query: 61  LSPSPHPTKLLYNYERAANGFSAHITAAQAAELRQVPGVISVIPDRIRQLHTTRTPHFLG 120
           L  SP P  LLY+Y RA +GFSA ++  Q A LR+ P VISVIPD+ R++HTT TP FLG
Sbjct: 61  LPSSPQPATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLG 120

Query: 121 LADNLGLWPDANYAEDVIIGVLDTGIWPERPSFSDEGLSPVPESWKGICETGDRFSASAC 180
            + N GLW ++NY EDVI+GVLDTGIWPE PSFSD GL P+P +WKG CE G  F AS+C
Sbjct: 121 FSQNSGLWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSC 180

Query: 181 NRKIIGARAYFYGY-ESNLGRSLQDSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYA 240
           NRK+IGARA++ GY     G     + + +S RDT+GHGTHTASTAAGS V NAS +QYA
Sbjct: 181 NRKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYA 240

Query: 241 RGEARGMASKARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSTGRAPAYYRD 300
           RG A GMASKARIAAYKICW  GC+DSDILAAMDQA+ADGVH+ISLSVG++G AP Y+ D
Sbjct: 241 RGTATGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTD 300

Query: 301 SIAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRV 360
           SIAIGAFGA +HG+VVSCSAGNSGP P TA NIAPWILTVGAST+DREF A+ I GDG+V
Sbjct: 301 SIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKV 360

Query: 361 FSGVSLYSGDPLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSS 420
           F+G SLY+G+ L DS+L LVY+GDCG R CY G L+SS V GKIV+CDRGGNARV KGS+
Sbjct: 361 FTGTSLYAGESLPDSQLSLVYSGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSA 420

Query: 421 VKTAGGLGMILANTEENGEELLADSHLIPGTMVGEIAGNKLKEYIHSDPNPTATIVFRGT 480
           VK AGG GMILANT E+GEEL ADSHL+P TMVG  AG+++++YI +  +PTA I F GT
Sbjct: 421 VKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGT 480

Query: 481 VIGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEF 540
           +IG SPP+PRVAAFSSRGPN+ T  ILKPDVIAPGVNILAGW+G   PT L IDPRRV+F
Sbjct: 481 LIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQF 540

Query: 541 NIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDTSGSPIKDLSTSKESN 600
           NIISGTSMSCPHVSG+AALLRKA P WSPAAIKSALVTT+Y ++ SG PI+DL+T K SN
Sbjct: 541 NIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSN 600

Query: 601 PFVHGAGHIDPNIALNPGLIYDINPQDYVSFLCSIGYDSKQIAVFVKESSYAKLCE-HKL 660
            F+HGAGH+DPN ALNPGL+YDI  ++YV+FLC++GY+   I VF+++ +    CE  KL
Sbjct: 601 SFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKL 660

Query: 661 SNPGNLNYPSFAVVFDSDAGEVVKYTRTVTNVGDEADAVYEVKVEAPEGVEISVLPKKLE 720
              G+LNYPSF+VVF S  GEVVKY R V NVG   DAVYEV V++P  VEI V P KL 
Sbjct: 661 RTAGDLNYPSFSVVFAS-TGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLA 720

Query: 721 FNANKTTQSYEISFTKI-----SGFTKSASFGSIQWSDGSHIVRSPIAVSFNSGYVAS 772
           F+  K+   YE++F  +      G      FGSI+W+DG H+V+SP+AV +  G V S
Sbjct: 721 FSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQWGQGSVQS 776

BLAST of Spg014889 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 785.8 bits (2028), Expect = 4.5e-226
Identity = 424/772 (54.92%), Postives = 536/772 (69.43%), Query Frame = 0

Query: 1   MASFHLCSSVFLFFFILCFVPVIFSRSPEDQQTFIVHVSKSEKPSLFSSHHHWHSSILES 60
           M+S  L S+   FF +LC      S S  DQ T+IVH++KS+ PS F  H +W+ S L S
Sbjct: 1   MSSSFLSSTA--FFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRS 60

Query: 61  LSPSPHPTKLLYNYERAANGFSAHITAAQAAELRQVPGVISVIPDRIRQLHTTRTPHFLG 120
           +S S    +LLY YE A +GFS  +T  +A  L   PGVISV+P+   +LHTTRTP FLG
Sbjct: 61  ISDS---AELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLG 120

Query: 121 LADNLG-LWPDANYAEDVIIGVLDTGIWPERPSFSDEGLSPVPESWKGICETGDRFSASA 180
           L ++   L+P+A    DV++GVLDTG+WPE  S+SDEG  P+P SWKG CE G  F+AS 
Sbjct: 121 LDEHTADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASL 180

Query: 181 CNRKIIGARAYFYGYESNLGRSLQDSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYA 240
           CNRK+IGAR +  GYES +G  + +S + +S RD DGHGTHT+STAAGS V+ AS   YA
Sbjct: 181 CNRKLIGARFFARGYESTMG-PIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYA 240

Query: 241 RGEARGMASKARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSTGRAPAYYRD 300
            G ARGMA +AR+A YK+CW  GCF SDILAA+D+AIAD V+++S+S+G  G    YYRD
Sbjct: 241 SGTARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLG--GGMSDYYRD 300

Query: 301 SIAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRV 360
            +AIGAF AM+ G++VSCSAGN+GP   +  N+APWI TVGA T+DR+F A  ILG+G+ 
Sbjct: 301 GVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKN 360

Query: 361 FSGVSLYSGDPLGDSKLPLVYAGDCGD----RYCYSGTLDSSKVAGKIVVCDRGGNARVA 420
           F+GVSL+ G+ L D  LP +YAG+  +      C +GTL   KV GKIV+CDRG NARV 
Sbjct: 361 FTGVSLFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQ 420

Query: 421 KGSSVKTAGGLGMILANTEENGEELLADSHLIPGTMVGEIAGNKLKEYIHSDPNPTATIV 480
           KG  VK AGG+GMILANT  NGEEL+AD+HL+P T VGE AG+ ++ Y+ +DPNPTA+I 
Sbjct: 421 KGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASIS 480

Query: 481 FRGTVIGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPR 540
             GTV+G   P+P VAAFSSRGPN  T  ILKPD+IAPGVNILA W+G + PT L+ D R
Sbjct: 481 ILGTVVG-VKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSR 540

Query: 541 RVEFNIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDTSGSPIKDLSTS 600
           RVEFNIISGTSMSCPHVSG+AALL+   P WSPAAI+SAL+TT+Y     G P+ D++T 
Sbjct: 541 RVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATG 600

Query: 601 KESNPFVHGAGHIDPNIALNPGLIYDINPQDYVSFLCSIGYDSKQIAVFVKESSYAKLCE 660
           K S PF HGAGH+ P  A NPGLIYD+  +DY+ FLC++ Y S QI   V   +Y   C+
Sbjct: 601 KPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIR-SVSRRNYT--CD 660

Query: 661 -HKLSNPGNLNYPSFAVVFDSDAGEVVKYTRTVTNVGDEADAVYEVKVEA-PEGVEISVL 720
             K  +  +LNYPSFAV  + D     KYTRTVT+VG      Y VKV +   GV+ISV 
Sbjct: 661 PSKSYSVADLNYPSFAV--NVDGVGAYKYTRTVTSVG--GAGTYSVKVTSETTGVKISVE 720

Query: 721 PKKLEFNANKTTQSYEISFTKISGF-TKSASFGSIQWSDGSHIVRSPIAVSF 765
           P  L F      +SY ++FT  S   + S SFGSI+WSDG H+V SP+A+S+
Sbjct: 721 PAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of Spg014889 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 748.0 bits (1930), Expect = 1.0e-214
Identity = 393/740 (53.11%), Postives = 502/740 (67.84%), Query Frame = 0

Query: 31  QQTFIVHVSKSEKPSLFSSHHHWHSSILESLSPSPHPTKLLYNYERAANGFSAHITAAQA 90
           ++T+I+ V+ S+KP  F +HH W++S L S S       LLY Y  + +GFSA++ + +A
Sbjct: 27  KKTYIIRVNHSDKPESFLTHHDWYTSQLNSES------SLLYTYTTSFHGFSAYLDSTEA 86

Query: 91  -AELRQVPGVISVIPDRIRQLHTTRTPHFLGLADNLGLWPDANYAEDVIIGVLDTGIWPE 150
            + L     ++ +  D +  LHTTRTP FLGL    G+    + +  VIIGVLDTG+WPE
Sbjct: 87  DSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPE 146

Query: 151 RPSFSDEGLSPVPESWKGICETGDRFSASACNRKIIGARAYFYGYESNLGRSLQDSSDFK 210
             SF D  +  +P  WKG CE+G  F +  CN+K+IGAR++  G++   G       +  
Sbjct: 147 SRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESV 206

Query: 211 SARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMASKARIAAYKICWEFGCFDSDIL 270
           S RD DGHGTHT++TAAGS V+NASF  YA G ARGMA++AR+A YK+CW  GCF SDIL
Sbjct: 207 SPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDIL 266

Query: 271 AAMDQAIADGVHIISLSVGSTGRAPAYYRDSIAIGAFGAMQHGVVVSCSAGNSGPGPYTA 330
           AAMD+AI DGV ++SLS+G  G AP YYRD+IAIGAF AM+ GV VSCSAGNSGP   + 
Sbjct: 267 AAMDRAILDGVDVLSLSLGG-GSAP-YYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASV 326

Query: 331 VNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYSGDPLGDSKLPLVY--AGDCGDR 390
            N+APW++TVGA T+DR+F A   LG+G+  +GVSLYSG  +G   L LVY         
Sbjct: 327 ANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYNKGNSSSSN 386

Query: 391 YCYSGTLDSSKVAGKIVVCDRGGNARVAKGSSVKTAGGLGMILANTEENGEELLADSHLI 450
            C  G+LDSS V GKIVVCDRG NARV KG+ V+ AGGLGMI+ANT  +GEEL+ADSHL+
Sbjct: 387 LCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLL 446

Query: 451 PGTMVGEIAGNKLKEYIHSDPNPTATIVFRGTVIGDSPPAPRVAAFSSRGPNYRTAEILK 510
           P   VG+  G+ L+EY+ SD  PTA +VF+GTV+ D  P+P VAAFSSRGPN  T EILK
Sbjct: 447 PAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVL-DVKPSPVVAAFSSRGPNTVTPEILK 506

Query: 511 PDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNIISGTSMSCPHVSGVAALLRKAFPTWS 570
           PDVI PGVNILAGWS    PT L  D RR +FNI+SGTSMSCPH+SG+A LL+ A P WS
Sbjct: 507 PDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWS 566

Query: 571 PAAIKSALVTTSYSLDTSGSPIKDLSTSKESNPFVHGAGHIDPNIALNPGLIYDINPQDY 630
           P+AIKSAL+TT+Y LD + +P+ D + +  SNP+ HG+GH+DP  AL+PGL+YDI+ ++Y
Sbjct: 567 PSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEY 626

Query: 631 VSFLCSIGYDSKQIAVFVKESSYAKLCEHKLSNPGNLNYPSFAVVFDSDAGEVVKYTRTV 690
           + FLCS+ Y    I   VK  S    C  K S+PG LNYPSF+V+F      VV+YTR V
Sbjct: 627 IRFLCSLDYTVDHIVAIVKRPSVN--CSKKFSDPGQLNYPSFSVLFGGK--RVVRYTREV 686

Query: 691 TNVGDEADAVYEVKVEAPEGVEISVLPKKLEFNANKTTQSYEISFTKISG--FTKSASFG 750
           TNVG  A +VY+V V     V ISV P KL F +    + Y ++F    G   T  A FG
Sbjct: 687 TNVG-AASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFG 746

Query: 751 SIQWSDGSHIVRSPIAVSFN 766
           SI WS+  H VRSP+A S+N
Sbjct: 747 SITWSNPQHEVRSPVAFSWN 752

BLAST of Spg014889 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 726.1 bits (1873), Expect = 4.2e-208
Identity = 389/776 (50.13%), Postives = 516/776 (66.49%), Query Frame = 0

Query: 11  FLFFFILCFVPVIFSRSPEDQQTFIVHVSKSEKPSLFSSHHHWHSSILESLSPSPHPTKL 70
           F FFF+L       S S  +  T+IVHV    KPS+F +H HW++S L SL+ S  P  +
Sbjct: 5   FYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSS--PPSI 64

Query: 71  LYNYERAANGFSAHITAAQAAELRQVPGVISVIPDRIRQLHTTRTPHFLGL--ADNLGLW 130
           ++ Y+   +GFSA +T+  A++L   P VISVIP+++R LHTTR+P FLGL   D  GL 
Sbjct: 65  IHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLL 124

Query: 131 PDANYAEDVIIGVLDTGIWPERPSFSDEGLSPVPESWKGICETGDRFSASACNRKIIGAR 190
            ++++  D++IGV+DTG+WPERPSF D GL PVP  WKG C     F  SACNRK++GAR
Sbjct: 125 EESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGAR 184

Query: 191 AYFYGYESNLGRSLQDSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMAS 250
            +  GYE+  G+ + ++++F+S RD+DGHGTHTAS +AG +V  AS   YA G A GMA 
Sbjct: 185 FFCGGYEATNGK-MNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAP 244

Query: 251 KARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSTGRAPAYYRDSIAIGAFGA 310
           KAR+AAYK+CW  GC+DSDILAA D A+ADGV +ISLSVG  G    YY D+IAIGAFGA
Sbjct: 245 KARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVG--GVVVPYYLDAIAIGAFGA 304

Query: 311 MQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYSG 370
           +  G+ VS SAGN GPG  T  N+APW+ TVGA TIDR+F A+V LG+G++ SGVS+Y G
Sbjct: 305 IDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGG 364

Query: 371 DPLGDSKL-PLVYAGDC--GDRY----CYSGTLDSSKVAGKIVVCDRGGNARVAKGSSVK 430
             L   ++ PLVY G    GD Y    C  G+LD + V GKIV+CDRG N+R  KG  V+
Sbjct: 365 PGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVR 424

Query: 431 TAGGLGMILANTEENGEELLADSHLIPGTMVGEIAGNKLKEYI------HSDPNPTATIV 490
             GGLGMI+AN   +GE L+AD H++P T VG   G++++ YI       S  +PTATIV
Sbjct: 425 KNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIV 484

Query: 491 FRGTVIGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPR 550
           F+GT +G   PAP VA+FS+RGPN  T EILKPDVIAPG+NILA W     P+ ++ D R
Sbjct: 485 FKGTRLG-IRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNR 544

Query: 551 RVEFNIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDTSGSPIKDLSTS 610
           R EFNI+SGTSM+CPHVSG+AALL+ A P WSPAAI+SAL+TT+Y++D SG P+ D ST 
Sbjct: 545 RTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTG 604

Query: 611 KESNPFVHGAGHIDPNIALNPGLIYDINPQDYVSFLCSIGYDSKQIAVFVKESSYAKLCE 670
             S+   +G+GH+ P  A++PGL+YDI   DY++FLC+  Y    I    +  +    C+
Sbjct: 605 NTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQAD---CD 664

Query: 671 --HKLSNPGNLNYPSFAVVFD--SDAGEVVKYTRTVTNVGDEADAVYEVKVEAPEGVEIS 730
              +  + GNLNYPSF+VVF    ++     + RTVTNVGD +D+VYE+K+  P G  ++
Sbjct: 665 GARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGD-SDSVYEIKIRPPRGTTVT 724

Query: 731 VLPKKLEFNANKTTQSYEI----SFTKISGFTKSASFGSIQWSDGSHIVRSPIAVS 764
           V P+KL F       S+ +    +  K+S    +   G I WSDG   V SP+ V+
Sbjct: 725 VEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVT 770

BLAST of Spg014889 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 715.3 bits (1845), Expect = 7.4e-205
Identity = 385/771 (49.94%), Postives = 501/771 (64.98%), Query Frame = 0

Query: 6   LCSSVFLFFFILCFVPVIFSRSPEDQQTFIVHVSKSEKPSLFSSHHHWHSSILESLSPSP 65
           + SS  +    L F P I   + +  +TFI  +     PS+F +H+HW+S+     S   
Sbjct: 1   MASSTIVLLLFLSF-PFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAEES--- 60

Query: 66  HPTKLLYNYERAANGFSAHITAAQAAELRQVPGVISVIPDRIRQLHTTRTPHFLGLADNL 125
              ++++ Y    +GFSA +T  +A  LR  P V++V  DR R+LHTTR+P FLGL +  
Sbjct: 61  ---RIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQK 120

Query: 126 GLWPDANYAEDVIIGVLDTGIWPERPSFSDEGLSPVPESWKGICETGDRFSASACNRKII 185
           GLW +++Y  DVIIGV DTGIWPER SFSD  L P+P+ W+G+CE+G RFS   CNRKII
Sbjct: 121 GLWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKII 180

Query: 186 GARAYFYGYESNLGRSLQDSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARG 245
           GAR +  G ++ +   +  + +F S RD DGHGTHT+STAAG     AS   YA G A+G
Sbjct: 181 GARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKG 240

Query: 246 MASKARIAAYKICW-EFGCFDSDILAAMDQAIADGVHIISLSV-GSTGRAPAYYRDSIAI 305
           +A KARIAAYK+CW + GC DSDILAA D A+ DGV +IS+S+ G  G    YY D IAI
Sbjct: 241 VAPKARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAI 300

Query: 306 GAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGV 365
           G++GA   G+ VS SAGN GP   +  N+APW+ TVGASTIDR F AD ILGDG    GV
Sbjct: 301 GSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGV 360

Query: 366 SLYSGDPLGDSKLPLVYAGDCG---DRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSSV 425
           SLY+G PL     P+VY G  G      C   TLD  +V GKIV+CDRG + RVAKG  V
Sbjct: 361 SLYAGVPLNGRMFPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVV 420

Query: 426 KTAGGLGMILANTEENGEELLADSHLIPGTMVGEIAGNKLKEYIHSDPNPTATIVFRGTV 485
           K AGG+GMILAN   NGE L+ D+HLIP   VG   G+++K Y  S PNP A+I FRGT+
Sbjct: 421 KKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTI 480

Query: 486 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFN 545
           +G   PAP +A+FS RGPN  + EILKPD+IAPGVNILA W+    PT L  DPR+ EFN
Sbjct: 481 VG-IKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFN 540

Query: 546 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDTSGSPIKDLSTSKESNP 605
           I+SGTSM+CPHVSG AALL+ A P WSPA I+SA++TT+  +D S   + D ST K + P
Sbjct: 541 ILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATP 600

Query: 606 FVHGAGHIDPNIALNPGLIYDINPQDYVSFLCSIGYDSKQIAVFVKESSYAKLCEHKLSN 665
           + +G+GH++   A+NPGL+YDI   DY++FLCSIGY  K I V  +  +  +    +  +
Sbjct: 601 YDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITR--TPVRCPTTRKPS 660

Query: 666 PGNLNYPSFAVVFDSDAGEVVKYT--RTVTNVGDEADAVYEVKVEAPEGVEISVLPKKLE 725
           PGNLNYPS   VF ++   +V  T  RT TNVG +A+AVY  ++E+P GV ++V P +L 
Sbjct: 661 PGNLNYPSITAVFPTNRRGLVSKTVIRTATNVG-QAEAVYRARIESPRGVTVTVKPPRLV 720

Query: 726 FNANKTTQSYEISFT-----KISGFTKSASFGSIQWSD-GSHIVRSPIAVS 764
           F +    +SY ++ T      + G T  A FGS+ W D G H+VRSPI V+
Sbjct: 721 FTSAVKRRSYAVTVTVNTRNVVLGET-GAVFGSVTWFDGGKHVVRSPIVVT 759

BLAST of Spg014889 vs. ExPASy TrEMBL
Match: A0A6J1HMN7 (subtilisin-like protease SBT1.4 OS=Cucurbita moschata OX=3662 GN=LOC111464338 PE=3 SV=1)

HSP 1 Score: 1403.3 bits (3631), Expect = 0.0e+00
Identity = 698/772 (90.41%), Postives = 737/772 (95.47%), Query Frame = 0

Query: 1   MASFHLCSSVFLFFFILCFVPVIFSRSPEDQQTFIVHVSKSEKPSLFSSHHHWHSSILES 60
           MA+ HLCSS F FF ILCFVPVIFSRS ++Q+TFIVHVSKSEKP+LFSSHHHWHSSILES
Sbjct: 1   MANLHLCSSGF-FFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILES 60

Query: 61  LSPSPHPTKLLYNYERAANGFSAHITAAQAAELRQVPGVISVIPDRIRQLHTTRTPHFLG 120
           LSPSPHPTKLLY+YE AANGFSA ITAAQAAELR++PGVISVIPDR+RQLHTTRTPHFLG
Sbjct: 61  LSPSPHPTKLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLG 120

Query: 121 LADNLGLWPDANYAEDVIIGVLDTGIWPERPSFSDEGLSPVPESWKGICETGDRFSASAC 180
           LAD+ GLW + NYA+DVIIGVLDTGIWPERPSFSDEGLSPVP +WKG C+TG  FSASAC
Sbjct: 121 LADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASAC 180

Query: 181 NRKIIGARAYFYGYESNLGRSLQDSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240
           NRKIIGARAYF GYES + RSLQ SSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR
Sbjct: 181 NRKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240

Query: 241 GEARGMASKARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSTGRAPAYYRDS 300
           GEARGMAS+ARIAAYKICWE GC+DSDILAAMDQAIADGVHIISLSVGS+GR PAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDS 300

Query: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360
           IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWI+TVGASTIDREFLADVILGDGRVF
Sbjct: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVF 360

Query: 361 SGVSLYSGDPLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSSV 420
           SGVSLYSGDPLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGS+V
Sbjct: 361 SGVSLYSGDPLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAV 420

Query: 421 KTAGGLGMILANTEENGEELLADSHLIPGTMVGEIAGNKLKEYIHSDPNPTATIVFRGTV 480
           KTAGGLGMILANTEENGEELLADSHLIPGTM+GEIAGN LK+YIHSDP+PTATIVFRGTV
Sbjct: 421 KTAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTV 480

Query: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFN 540
           IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFN
Sbjct: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDTSGSPIKDLSTSKESNP 600
           IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLD+SG PI DLSTSK+SNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNP 600

Query: 601 FVHGAGHIDPNIALNPGLIYDINPQDYVSFLCSIGYDSKQIAVFVKESSYAKLCEHKLSN 660
           FVHGAGHIDPN ALNPGLIYD+NPQDYVSFLCSIGYDS+QIAVFVK+S YA+LCEHKL++
Sbjct: 601 FVHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAQLCEHKLTS 660

Query: 661 PGNLNYPSFAVVFDSDAGEVVKYTRTVTNVGDEADAVYEVKVEAPEGVEISVLPKKLEFN 720
           PGNLNYPSFAVVFDSD  EVVKYTRTVTNVGDE DAVYEVKVE+P+GVEI+VLP KLEF+
Sbjct: 661 PGNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPQGVEITVLPNKLEFS 720

Query: 721 ANKTTQSYEISFTKISGFTKSASFGSIQWSDGSHIVRSPIAVSFNSGYVASM 773
           A   TQSYEI+FTKI+G   SASFGSIQWSDGSHIVRSP+AVSFNSGY+ASM
Sbjct: 721 AKDRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM 771

BLAST of Spg014889 vs. ExPASy TrEMBL
Match: A0A6J1KIR1 (subtilisin-like protease SBT1.4 OS=Cucurbita maxima OX=3661 GN=LOC111494328 PE=3 SV=1)

HSP 1 Score: 1402.1 bits (3628), Expect = 0.0e+00
Identity = 696/772 (90.16%), Postives = 738/772 (95.60%), Query Frame = 0

Query: 1   MASFHLCSSVFLFFFILCFVPVIFSRSPEDQQTFIVHVSKSEKPSLFSSHHHWHSSILES 60
           MA+ HLCSSVF FF ILCFVPVIFSRS ++Q+TFIVHVSKSEKP+LFSSHHHWHSSIL+S
Sbjct: 1   MANLHLCSSVF-FFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILDS 60

Query: 61  LSPSPHPTKLLYNYERAANGFSAHITAAQAAELRQVPGVISVIPDRIRQLHTTRTPHFLG 120
           LSPSPHPTKLLYNYE AANGFSA ITAAQAAELR++PGVISVIPDR+RQLHTTRTPHFLG
Sbjct: 61  LSPSPHPTKLLYNYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLG 120

Query: 121 LADNLGLWPDANYAEDVIIGVLDTGIWPERPSFSDEGLSPVPESWKGICETGDRFSASAC 180
           LAD+ GLW + NYA+DVIIGVLDTGIWPERPSFSDEGLSPVP +WKG C+TG  FSASAC
Sbjct: 121 LADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASAC 180

Query: 181 NRKIIGARAYFYGYESNLGRSLQDSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240
           NRKIIGARAYF GYES + RSLQ SSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR
Sbjct: 181 NRKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240

Query: 241 GEARGMASKARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSTGRAPAYYRDS 300
           GEARGMA++ARIAAYKICWE GC+DSDILAAMDQAIADGVHIISLSVGS+GR PAYYRDS
Sbjct: 241 GEARGMANRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDS 300

Query: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360
           IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWI+TVGASTIDREFLADVILGDGRVF
Sbjct: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVF 360

Query: 361 SGVSLYSGDPLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSSV 420
           SGVSLYSGDPLGDSKLPLVYAGDCG+RYCYSGTLDSSKVAGKIVVCDRGGNARVAKGS+V
Sbjct: 361 SGVSLYSGDPLGDSKLPLVYAGDCGNRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSTV 420

Query: 421 KTAGGLGMILANTEENGEELLADSHLIPGTMVGEIAGNKLKEYIHSDPNPTATIVFRGTV 480
           KTAGGLGMILANTEENGEELLADSHLIPGTM+GEIAGN LK+YIHSDP+PTATIVFRGTV
Sbjct: 421 KTAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTV 480

Query: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFN 540
           IGDSPPAPRVAAFSSRGPN+RTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFN
Sbjct: 481 IGDSPPAPRVAAFSSRGPNFRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDTSGSPIKDLSTSKESNP 600
           IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLD+SG PI DLSTSK+SNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNP 600

Query: 601 FVHGAGHIDPNIALNPGLIYDINPQDYVSFLCSIGYDSKQIAVFVKESSYAKLCEHKLSN 660
           FVHGAGHIDPN ALNPGLIYD+NPQDYVSFLCSIGYDS+QIAVFVK+S YA+LC+HKL++
Sbjct: 601 FVHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAELCKHKLTS 660

Query: 661 PGNLNYPSFAVVFDSDAGEVVKYTRTVTNVGDEADAVYEVKVEAPEGVEISVLPKKLEFN 720
           PGNLNYPSFAVVFDSD  EVVKYTRTVTNVGDE DAVYEVKVE+PEGVEI+VLP KLEF+
Sbjct: 661 PGNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPEGVEITVLPNKLEFS 720

Query: 721 ANKTTQSYEISFTKISGFTKSASFGSIQWSDGSHIVRSPIAVSFNSGYVASM 773
           A   TQSYEI+FTKI+G   SASFGSIQWSDGSHIVRSP+AVSFNSGY+ASM
Sbjct: 721 AKNRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM 771

BLAST of Spg014889 vs. ExPASy TrEMBL
Match: A0A6J1GQK5 (subtilisin-like protease SBT1.4 OS=Cucurbita moschata OX=3662 GN=LOC111456188 PE=3 SV=1)

HSP 1 Score: 1392.5 bits (3603), Expect = 0.0e+00
Identity = 685/772 (88.73%), Postives = 732/772 (94.82%), Query Frame = 0

Query: 1   MASFHLCSSVFLFFFILCFVPVIFSRSPEDQQTFIVHVSKSEKPSLFSSHHHWHSSILES 60
           MA+FH CSSVF FFF LCF+PVIFSRSP+ Q+TFIVHVSK EKPSLFSSH+ WHSSIL+S
Sbjct: 1   MANFHRCSSVFFFFFFLCFLPVIFSRSPDKQETFIVHVSKLEKPSLFSSHYRWHSSILDS 60

Query: 61  LSPSPHPTKLLYNYERAANGFSAHITAAQAAELRQVPGVISVIPDRIRQLHTTRTPHFLG 120
           LSPS  P KLLYNYERA NGF+A IT AQAAELR+VPGV+SVIPDR+RQLHTTRTPHFLG
Sbjct: 61  LSPSSRPRKLLYNYERAVNGFAARITDAQAAELRRVPGVVSVIPDRMRQLHTTRTPHFLG 120

Query: 121 LADNLGLWPDANYAEDVIIGVLDTGIWPERPSFSDEGLSPVPESWKGICETGDRFSASAC 180
           LADNLGLWPD NYA+DVIIGVLDTGIWPERPSFSDEGLSPVP SWKG CE G+ FSA+ C
Sbjct: 121 LADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCEAGEGFSATTC 180

Query: 181 NRKIIGARAYFYGYESNLGRSLQDSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240
           NRKIIGARA+F+GYESN+GR L   SDFKSARD+DGHGTHTASTAAGSFVKNASFFQYAR
Sbjct: 181 NRKIIGARAFFHGYESNIGRPLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYAR 240

Query: 241 GEARGMASKARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSTGRAPAYYRDS 300
           GEARGMAS+ARIAAYKICWEFGC+DSDILAAMDQAIADGVHIISLSVGS+GRAPAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 300

Query: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360
           IAIGAFGAMQHG+VVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF
Sbjct: 301 IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360

Query: 361 SGVSLYSGDPLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSSV 420
           SGVSLYSGDPLGDSKLPLVY GDCG+RYCYSG+LD SKVAGKIVVCDRGGNARVAKGS+V
Sbjct: 361 SGVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAV 420

Query: 421 KTAGGLGMILANTEENGEELLADSHLIPGTMVGEIAGNKLKEYIHSDPNPTATIVFRGTV 480
           K+AGGLG+ILANTEENGEELLADSHLIPGTM+GEIAGNKL++YIHSDPNP+ATIVFRGT+
Sbjct: 421 KSAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTI 480

Query: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFN 540
           I DSPP+PRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGY+SP+SLSID RRVEFN
Sbjct: 481 ISDSPPSPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDTSGSPIKDLSTSKESNP 600
           IISGTSMSCPHVSGVAALLRKAFPTW+PAAIKSAL+TTSYSLDTSG PIKDLSTS +SNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGRPIKDLSTSNDSNP 600

Query: 601 FVHGAGHIDPNIALNPGLIYDINPQDYVSFLCSIGYDSKQIAVFVKESSYAKLCEHKLSN 660
           FVHG GHIDPN ALNPGLIYD+NPQDYVSFLCSIGYDS+QIAVFVK+SSY+ LCEHKLSN
Sbjct: 601 FVHGGGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSN 660

Query: 661 PGNLNYPSFAVVFDSDAGEVVKYTRTVTNVGDEADAVYEVKVEAPEGVEISVLPKKLEFN 720
           PGNLNYPSFAVVF S+  EVVKYTRTVTNVGDEADAVYEVKVEAP+GVEISVLP KLEF+
Sbjct: 661 PGNLNYPSFAVVFTSNGEEVVKYTRTVTNVGDEADAVYEVKVEAPQGVEISVLPNKLEFS 720

Query: 721 ANKTTQSYEISFTKISGFTKSASFGSIQWSDGSHIVRSPIAVSFNSGYVASM 773
           A KTTQSYEI+FTKISGF  SASFGSIQWSDGSHIVRSPIAVSFN+  +ASM
Sbjct: 721 AKKTTQSYEITFTKISGFKNSASFGSIQWSDGSHIVRSPIAVSFNTASIASM 772

BLAST of Spg014889 vs. ExPASy TrEMBL
Match: A0A6J1JUV1 (subtilisin-like protease SBT1.4 OS=Cucurbita maxima OX=3661 GN=LOC111488562 PE=3 SV=1)

HSP 1 Score: 1388.6 bits (3593), Expect = 0.0e+00
Identity = 686/772 (88.86%), Postives = 732/772 (94.82%), Query Frame = 0

Query: 1   MASFHLCSSVFLFFFILCFVPVIFSRSPEDQQTFIVHVSKSEKPSLFSSHHHWHSSILES 60
           MA+FH CSSVF FFF LCF+PVIFSRSP+ Q+TFIVHVSKSEKPSLFSSH+ WHSSIL+S
Sbjct: 1   MANFHRCSSVF-FFFFLCFLPVIFSRSPDKQETFIVHVSKSEKPSLFSSHYRWHSSILDS 60

Query: 61  LSPSPHPTKLLYNYERAANGFSAHITAAQAAELRQVPGVISVIPDRIRQLHTTRTPHFLG 120
           LSPS  P KLLYNYERA NGF+A ITA QAAELR+VPGV+ VIPDR+RQLHTTRTPHFLG
Sbjct: 61  LSPSSRPRKLLYNYERAVNGFAARITADQAAELRRVPGVVLVIPDRMRQLHTTRTPHFLG 120

Query: 121 LADNLGLWPDANYAEDVIIGVLDTGIWPERPSFSDEGLSPVPESWKGICETGDRFSASAC 180
           LADNLGLWPD NYA+DVIIGVLDTGIWPE PSFSDEGLSPVP SWKG CE G+ FSA+ C
Sbjct: 121 LADNLGLWPDTNYADDVIIGVLDTGIWPEHPSFSDEGLSPVPASWKGTCEAGEGFSATTC 180

Query: 181 NRKIIGARAYFYGYESNLGRSLQDSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240
           NRKIIGARA+F+GYESN+GR L   SDFKSARD+DGHGTHTASTAAGSFVKNASFFQYAR
Sbjct: 181 NRKIIGARAFFHGYESNIGRRLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYAR 240

Query: 241 GEARGMASKARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSTGRAPAYYRDS 300
           GEARGMAS+ARIAAYKICWEFGC+DSDILAAMDQAIADGVHIISLSVGS+GRAPAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 300

Query: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360
           IAIGAFGAMQHG+VVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF
Sbjct: 301 IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360

Query: 361 SGVSLYSGDPLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSSV 420
           SGVSLYSGDPLGDSKLPLVY GDCG+RYCYSG+LD SKVAGKIVVCDRGGNARVAKGS+V
Sbjct: 361 SGVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAV 420

Query: 421 KTAGGLGMILANTEENGEELLADSHLIPGTMVGEIAGNKLKEYIHSDPNPTATIVFRGTV 480
           K+AGGLG+ILANTEENGEELLADSHLIPGTM+GEIAGNKL++YIHSDPNP+ATIVFRGTV
Sbjct: 421 KSAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTV 480

Query: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFN 540
           IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGY+SP+SLSID RRVEFN
Sbjct: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDTSGSPIKDLSTSKESNP 600
           IISGTSMSCPHVSGVAALLRKAFPTW+PAAIKSAL+TTSYSLDTSGSPIKDLSTS +SNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGSPIKDLSTSNDSNP 600

Query: 601 FVHGAGHIDPNIALNPGLIYDINPQDYVSFLCSIGYDSKQIAVFVKESSYAKLCEHKLSN 660
           FVHG GHIDPN ALNPGLIYD+NP DYVSFLCSIGYDS+QIAVFVK+SSY+ LCEHKLSN
Sbjct: 601 FVHGGGHIDPNKALNPGLIYDLNPHDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSN 660

Query: 661 PGNLNYPSFAVVFDSDAGEVVKYTRTVTNVGDEADAVYEVKVEAPEGVEISVLPKKLEFN 720
           PGNLNYPSFAVVF S+  EVVKYTRTVTNVGDEADAVYEVKVEAP+GVEISVLP KLEF+
Sbjct: 661 PGNLNYPSFAVVFTSNGEEVVKYTRTVTNVGDEADAVYEVKVEAPQGVEISVLPNKLEFS 720

Query: 721 ANKTTQSYEISFTKISGFTKSASFGSIQWSDGSHIVRSPIAVSFNSGYVASM 773
           A KTT+SYEI+FTKISGF  SASFGSIQWSDGSHIVRSPIAVSFN+  +ASM
Sbjct: 721 AKKTTESYEITFTKISGFKNSASFGSIQWSDGSHIVRSPIAVSFNTASIASM 771

BLAST of Spg014889 vs. ExPASy TrEMBL
Match: A0A1S3CGN8 (LOW QUALITY PROTEIN: subtilisin-like protease SBT1.4 OS=Cucumis melo OX=3656 GN=LOC103500685 PE=3 SV=1)

HSP 1 Score: 1366.7 bits (3536), Expect = 0.0e+00
Identity = 677/772 (87.69%), Postives = 725/772 (93.91%), Query Frame = 0

Query: 1   MASFHLCSSVFLFFFILCFVPVIFSRSPEDQQTFIVHVSKSEKPSLFSSHHHWHSSILES 60
           MA+FHL SS F FFF+L   P++FSRS E Q+T+IVHVSKSEKPSLFSSHHHWHSSILES
Sbjct: 1   MANFHLPSSSFFFFFVLFVFPLVFSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILES 60

Query: 61  LSPSPHPTKLLYNYERAANGFSAHITAAQAAELRQVPGVISVIPDRIRQLHTTRTPHFLG 120
           LSPSPHPTKLLYNYERAANGFSA IT AQA ELR+VPG+ISVIPDRIRQLHTTRTPHFLG
Sbjct: 61  LSPSPHPTKLLYNYERAANGFSARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLG 120

Query: 121 LADNLGLWPDANYAEDVIIGVLDTGIWPERPSFSDEGLSPVPESWKGICETGDRFSASAC 180
           LADNLGLW D NYA+DVIIGVLDTGIWPERPSFSDEGL+PVP  WKG C+TG+  SA AC
Sbjct: 121 LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALAC 180

Query: 181 NRKIIGARAYFYGYESNLGRSLQDSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240
           NRKIIGARA+F GYESNL  SL+ SSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR
Sbjct: 181 NRKIIGARAFFXGYESNLRGSLKGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240

Query: 241 GEARGMASKARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSTGRAPAYYRDS 300
           GEARGMAS+ARIAAYKICWEFGC+DSDILAAMDQAI+DGV +ISLSVGS+GRAPAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS 300

Query: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360
           IAIGAFGAMQHG+VVSCSAGNSGPGPYTAVNIAPWI+TVGASTIDREFLADVILGD RVF
Sbjct: 301 IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVF 360

Query: 361 SGVSLYSGDPLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSSV 420
           SGVSLYSGDPLGDSKLPLVY GDCG RYCYSG+LDSSKVAG+IVVCDRGGNARVAKG +V
Sbjct: 361 SGVSLYSGDPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGRIVVCDRGGNARVAKGGAV 420

Query: 421 KTAGGLGMILANTEENGEELLADSHLIPGTMVGEIAGNKLKEYIHSDPNPTATIVFRGTV 480
           K+AGGLGM+LANTEENGEELLADSHLIPGTMVGEIAGNKL++YIH+DPNPTATIVFRGTV
Sbjct: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTV 480

Query: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFN 540
           IGDSPPAP+VA+FSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPT L+IDPRRVEFN
Sbjct: 481 IGDSPPAPKVASFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDTSGSPIKDLSTSKESNP 600
           IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSAL+TTSYSLD+SGSPIKDL+TS+ESNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNP 600

Query: 601 FVHGAGHIDPNIALNPGLIYDINPQDYVSFLCSIGYDSKQIAVFVKESSYAKLCEHKLSN 660
           FVHGAGHIDPN ALNPGLIYD+ PQDYVSFLCSIGYDSKQIAVFVK SSY++LCEHKLSN
Sbjct: 601 FVHGAGHIDPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCEHKLSN 660

Query: 661 PGNLNYPSFAVVFDSDAGEVVKYTRTVTNVGDEADAVYEVKVEAPEGVEISVLPKKLEFN 720
           PGNLNYPSF+VVFD   GEVVKYTRTVTNVGDE + VY VKVEAP+GVEISV+P KLEFN
Sbjct: 661 PGNLNYPSFSVVFDD--GEVVKYTRTVTNVGDETEVVYGVKVEAPQGVEISVVPNKLEFN 720

Query: 721 ANKTTQSYEISFTKISGFTKSASFGSIQWSDGSHIVRSPIAVSFNSGYVASM 773
             KTT SYEI+FTKI+GF +SASFGSIQWSDG H VRSPIAVSF +G +ASM
Sbjct: 721 KEKTTLSYEITFTKINGFKESASFGSIQWSDGIHNVRSPIAVSFKTGSIASM 770

BLAST of Spg014889 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 987.6 bits (2552), Expect = 5.5e-288
Identity = 496/778 (63.75%), Postives = 596/778 (76.61%), Query Frame = 0

Query: 1   MASFHLCSSVFLFFFILCFVPVIFSRSPEDQQTFIVHVSKSEKPSLFSSHHHWHSSILES 60
           MA   L S  F+F  +LCF     S S +  +++IVHV +S KPSLFSSH++WH S+L S
Sbjct: 1   MAKLSLSSIFFVFPLLLCFFSP-SSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRS 60

Query: 61  LSPSPHPTKLLYNYERAANGFSAHITAAQAAELRQVPGVISVIPDRIRQLHTTRTPHFLG 120
           L  SP P  LLY+Y RA +GFSA ++  Q A LR+ P VISVIPD+ R++HTT TP FLG
Sbjct: 61  LPSSPQPATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLG 120

Query: 121 LADNLGLWPDANYAEDVIIGVLDTGIWPERPSFSDEGLSPVPESWKGICETGDRFSASAC 180
            + N GLW ++NY EDVI+GVLDTGIWPE PSFSD GL P+P +WKG CE G  F AS+C
Sbjct: 121 FSQNSGLWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSC 180

Query: 181 NRKIIGARAYFYGY-ESNLGRSLQDSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYA 240
           NRK+IGARA++ GY     G     + + +S RDT+GHGTHTASTAAGS V NAS +QYA
Sbjct: 181 NRKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYA 240

Query: 241 RGEARGMASKARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSTGRAPAYYRD 300
           RG A GMASKARIAAYKICW  GC+DSDILAAMDQA+ADGVH+ISLSVG++G AP Y+ D
Sbjct: 241 RGTATGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTD 300

Query: 301 SIAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRV 360
           SIAIGAFGA +HG+VVSCSAGNSGP P TA NIAPWILTVGAST+DREF A+ I GDG+V
Sbjct: 301 SIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKV 360

Query: 361 FSGVSLYSGDPLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSS 420
           F+G SLY+G+ L DS+L LVY+GDCG R CY G L+SS V GKIV+CDRGGNARV KGS+
Sbjct: 361 FTGTSLYAGESLPDSQLSLVYSGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSA 420

Query: 421 VKTAGGLGMILANTEENGEELLADSHLIPGTMVGEIAGNKLKEYIHSDPNPTATIVFRGT 480
           VK AGG GMILANT E+GEEL ADSHL+P TMVG  AG+++++YI +  +PTA I F GT
Sbjct: 421 VKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGT 480

Query: 481 VIGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEF 540
           +IG SPP+PRVAAFSSRGPN+ T  ILKPDVIAPGVNILAGW+G   PT L IDPRRV+F
Sbjct: 481 LIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQF 540

Query: 541 NIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDTSGSPIKDLSTSKESN 600
           NIISGTSMSCPHVSG+AALLRKA P WSPAAIKSALVTT+Y ++ SG PI+DL+T K SN
Sbjct: 541 NIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSN 600

Query: 601 PFVHGAGHIDPNIALNPGLIYDINPQDYVSFLCSIGYDSKQIAVFVKESSYAKLCE-HKL 660
            F+HGAGH+DPN ALNPGL+YDI  ++YV+FLC++GY+   I VF+++ +    CE  KL
Sbjct: 601 SFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKL 660

Query: 661 SNPGNLNYPSFAVVFDSDAGEVVKYTRTVTNVGDEADAVYEVKVEAPEGVEISVLPKKLE 720
              G+LNYPSF+VVF S  GEVVKY R V NVG   DAVYEV V++P  VEI V P KL 
Sbjct: 661 RTAGDLNYPSFSVVFAS-TGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLA 720

Query: 721 FNANKTTQSYEISFTKI-----SGFTKSASFGSIQWSDGSHIVRSPIAVSFNSGYVAS 772
           F+  K+   YE++F  +      G      FGSI+W+DG H+V+SP+AV +  G V S
Sbjct: 721 FSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQWGQGSVQS 776

BLAST of Spg014889 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 785.8 bits (2028), Expect = 3.2e-227
Identity = 424/772 (54.92%), Postives = 536/772 (69.43%), Query Frame = 0

Query: 1   MASFHLCSSVFLFFFILCFVPVIFSRSPEDQQTFIVHVSKSEKPSLFSSHHHWHSSILES 60
           M+S  L S+   FF +LC      S S  DQ T+IVH++KS+ PS F  H +W+ S L S
Sbjct: 1   MSSSFLSSTA--FFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRS 60

Query: 61  LSPSPHPTKLLYNYERAANGFSAHITAAQAAELRQVPGVISVIPDRIRQLHTTRTPHFLG 120
           +S S    +LLY YE A +GFS  +T  +A  L   PGVISV+P+   +LHTTRTP FLG
Sbjct: 61  ISDS---AELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLG 120

Query: 121 LADNLG-LWPDANYAEDVIIGVLDTGIWPERPSFSDEGLSPVPESWKGICETGDRFSASA 180
           L ++   L+P+A    DV++GVLDTG+WPE  S+SDEG  P+P SWKG CE G  F+AS 
Sbjct: 121 LDEHTADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASL 180

Query: 181 CNRKIIGARAYFYGYESNLGRSLQDSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYA 240
           CNRK+IGAR +  GYES +G  + +S + +S RD DGHGTHT+STAAGS V+ AS   YA
Sbjct: 181 CNRKLIGARFFARGYESTMG-PIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYA 240

Query: 241 RGEARGMASKARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSTGRAPAYYRD 300
            G ARGMA +AR+A YK+CW  GCF SDILAA+D+AIAD V+++S+S+G  G    YYRD
Sbjct: 241 SGTARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLG--GGMSDYYRD 300

Query: 301 SIAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRV 360
            +AIGAF AM+ G++VSCSAGN+GP   +  N+APWI TVGA T+DR+F A  ILG+G+ 
Sbjct: 301 GVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKN 360

Query: 361 FSGVSLYSGDPLGDSKLPLVYAGDCGD----RYCYSGTLDSSKVAGKIVVCDRGGNARVA 420
           F+GVSL+ G+ L D  LP +YAG+  +      C +GTL   KV GKIV+CDRG NARV 
Sbjct: 361 FTGVSLFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQ 420

Query: 421 KGSSVKTAGGLGMILANTEENGEELLADSHLIPGTMVGEIAGNKLKEYIHSDPNPTATIV 480
           KG  VK AGG+GMILANT  NGEEL+AD+HL+P T VGE AG+ ++ Y+ +DPNPTA+I 
Sbjct: 421 KGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASIS 480

Query: 481 FRGTVIGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPR 540
             GTV+G   P+P VAAFSSRGPN  T  ILKPD+IAPGVNILA W+G + PT L+ D R
Sbjct: 481 ILGTVVG-VKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSR 540

Query: 541 RVEFNIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDTSGSPIKDLSTS 600
           RVEFNIISGTSMSCPHVSG+AALL+   P WSPAAI+SAL+TT+Y     G P+ D++T 
Sbjct: 541 RVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATG 600

Query: 601 KESNPFVHGAGHIDPNIALNPGLIYDINPQDYVSFLCSIGYDSKQIAVFVKESSYAKLCE 660
           K S PF HGAGH+ P  A NPGLIYD+  +DY+ FLC++ Y S QI   V   +Y   C+
Sbjct: 601 KPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIR-SVSRRNYT--CD 660

Query: 661 -HKLSNPGNLNYPSFAVVFDSDAGEVVKYTRTVTNVGDEADAVYEVKVEA-PEGVEISVL 720
             K  +  +LNYPSFAV  + D     KYTRTVT+VG      Y VKV +   GV+ISV 
Sbjct: 661 PSKSYSVADLNYPSFAV--NVDGVGAYKYTRTVTSVG--GAGTYSVKVTSETTGVKISVE 720

Query: 721 PKKLEFNANKTTQSYEISFTKISGF-TKSASFGSIQWSDGSHIVRSPIAVSF 765
           P  L F      +SY ++FT  S   + S SFGSI+WSDG H+V SP+A+S+
Sbjct: 721 PAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of Spg014889 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 748.0 bits (1930), Expect = 7.3e-216
Identity = 393/740 (53.11%), Postives = 502/740 (67.84%), Query Frame = 0

Query: 31  QQTFIVHVSKSEKPSLFSSHHHWHSSILESLSPSPHPTKLLYNYERAANGFSAHITAAQA 90
           ++T+I+ V+ S+KP  F +HH W++S L S S       LLY Y  + +GFSA++ + +A
Sbjct: 27  KKTYIIRVNHSDKPESFLTHHDWYTSQLNSES------SLLYTYTTSFHGFSAYLDSTEA 86

Query: 91  -AELRQVPGVISVIPDRIRQLHTTRTPHFLGLADNLGLWPDANYAEDVIIGVLDTGIWPE 150
            + L     ++ +  D +  LHTTRTP FLGL    G+    + +  VIIGVLDTG+WPE
Sbjct: 87  DSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPE 146

Query: 151 RPSFSDEGLSPVPESWKGICETGDRFSASACNRKIIGARAYFYGYESNLGRSLQDSSDFK 210
             SF D  +  +P  WKG CE+G  F +  CN+K+IGAR++  G++   G       +  
Sbjct: 147 SRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESV 206

Query: 211 SARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMASKARIAAYKICWEFGCFDSDIL 270
           S RD DGHGTHT++TAAGS V+NASF  YA G ARGMA++AR+A YK+CW  GCF SDIL
Sbjct: 207 SPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDIL 266

Query: 271 AAMDQAIADGVHIISLSVGSTGRAPAYYRDSIAIGAFGAMQHGVVVSCSAGNSGPGPYTA 330
           AAMD+AI DGV ++SLS+G  G AP YYRD+IAIGAF AM+ GV VSCSAGNSGP   + 
Sbjct: 267 AAMDRAILDGVDVLSLSLGG-GSAP-YYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASV 326

Query: 331 VNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYSGDPLGDSKLPLVY--AGDCGDR 390
            N+APW++TVGA T+DR+F A   LG+G+  +GVSLYSG  +G   L LVY         
Sbjct: 327 ANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYNKGNSSSSN 386

Query: 391 YCYSGTLDSSKVAGKIVVCDRGGNARVAKGSSVKTAGGLGMILANTEENGEELLADSHLI 450
            C  G+LDSS V GKIVVCDRG NARV KG+ V+ AGGLGMI+ANT  +GEEL+ADSHL+
Sbjct: 387 LCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLL 446

Query: 451 PGTMVGEIAGNKLKEYIHSDPNPTATIVFRGTVIGDSPPAPRVAAFSSRGPNYRTAEILK 510
           P   VG+  G+ L+EY+ SD  PTA +VF+GTV+ D  P+P VAAFSSRGPN  T EILK
Sbjct: 447 PAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVL-DVKPSPVVAAFSSRGPNTVTPEILK 506

Query: 511 PDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNIISGTSMSCPHVSGVAALLRKAFPTWS 570
           PDVI PGVNILAGWS    PT L  D RR +FNI+SGTSMSCPH+SG+A LL+ A P WS
Sbjct: 507 PDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWS 566

Query: 571 PAAIKSALVTTSYSLDTSGSPIKDLSTSKESNPFVHGAGHIDPNIALNPGLIYDINPQDY 630
           P+AIKSAL+TT+Y LD + +P+ D + +  SNP+ HG+GH+DP  AL+PGL+YDI+ ++Y
Sbjct: 567 PSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEY 626

Query: 631 VSFLCSIGYDSKQIAVFVKESSYAKLCEHKLSNPGNLNYPSFAVVFDSDAGEVVKYTRTV 690
           + FLCS+ Y    I   VK  S    C  K S+PG LNYPSF+V+F      VV+YTR V
Sbjct: 627 IRFLCSLDYTVDHIVAIVKRPSVN--CSKKFSDPGQLNYPSFSVLFGGK--RVVRYTREV 686

Query: 691 TNVGDEADAVYEVKVEAPEGVEISVLPKKLEFNANKTTQSYEISFTKISG--FTKSASFG 750
           TNVG  A +VY+V V     V ISV P KL F +    + Y ++F    G   T  A FG
Sbjct: 687 TNVG-AASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFG 746

Query: 751 SIQWSDGSHIVRSPIAVSFN 766
           SI WS+  H VRSP+A S+N
Sbjct: 747 SITWSNPQHEVRSPVAFSWN 752

BLAST of Spg014889 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 726.1 bits (1873), Expect = 3.0e-209
Identity = 389/776 (50.13%), Postives = 516/776 (66.49%), Query Frame = 0

Query: 11  FLFFFILCFVPVIFSRSPEDQQTFIVHVSKSEKPSLFSSHHHWHSSILESLSPSPHPTKL 70
           F FFF+L       S S  +  T+IVHV    KPS+F +H HW++S L SL+ S  P  +
Sbjct: 5   FYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSS--PPSI 64

Query: 71  LYNYERAANGFSAHITAAQAAELRQVPGVISVIPDRIRQLHTTRTPHFLGL--ADNLGLW 130
           ++ Y+   +GFSA +T+  A++L   P VISVIP+++R LHTTR+P FLGL   D  GL 
Sbjct: 65  IHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLL 124

Query: 131 PDANYAEDVIIGVLDTGIWPERPSFSDEGLSPVPESWKGICETGDRFSASACNRKIIGAR 190
            ++++  D++IGV+DTG+WPERPSF D GL PVP  WKG C     F  SACNRK++GAR
Sbjct: 125 EESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGAR 184

Query: 191 AYFYGYESNLGRSLQDSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMAS 250
            +  GYE+  G+ + ++++F+S RD+DGHGTHTAS +AG +V  AS   YA G A GMA 
Sbjct: 185 FFCGGYEATNGK-MNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAP 244

Query: 251 KARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSTGRAPAYYRDSIAIGAFGA 310
           KAR+AAYK+CW  GC+DSDILAA D A+ADGV +ISLSVG  G    YY D+IAIGAFGA
Sbjct: 245 KARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVG--GVVVPYYLDAIAIGAFGA 304

Query: 311 MQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGVSLYSG 370
           +  G+ VS SAGN GPG  T  N+APW+ TVGA TIDR+F A+V LG+G++ SGVS+Y G
Sbjct: 305 IDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGG 364

Query: 371 DPLGDSKL-PLVYAGDC--GDRY----CYSGTLDSSKVAGKIVVCDRGGNARVAKGSSVK 430
             L   ++ PLVY G    GD Y    C  G+LD + V GKIV+CDRG N+R  KG  V+
Sbjct: 365 PGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVR 424

Query: 431 TAGGLGMILANTEENGEELLADSHLIPGTMVGEIAGNKLKEYI------HSDPNPTATIV 490
             GGLGMI+AN   +GE L+AD H++P T VG   G++++ YI       S  +PTATIV
Sbjct: 425 KNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIV 484

Query: 491 FRGTVIGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPR 550
           F+GT +G   PAP VA+FS+RGPN  T EILKPDVIAPG+NILA W     P+ ++ D R
Sbjct: 485 FKGTRLG-IRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNR 544

Query: 551 RVEFNIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDTSGSPIKDLSTS 610
           R EFNI+SGTSM+CPHVSG+AALL+ A P WSPAAI+SAL+TT+Y++D SG P+ D ST 
Sbjct: 545 RTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTG 604

Query: 611 KESNPFVHGAGHIDPNIALNPGLIYDINPQDYVSFLCSIGYDSKQIAVFVKESSYAKLCE 670
             S+   +G+GH+ P  A++PGL+YDI   DY++FLC+  Y    I    +  +    C+
Sbjct: 605 NTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQAD---CD 664

Query: 671 --HKLSNPGNLNYPSFAVVFD--SDAGEVVKYTRTVTNVGDEADAVYEVKVEAPEGVEIS 730
              +  + GNLNYPSF+VVF    ++     + RTVTNVGD +D+VYE+K+  P G  ++
Sbjct: 665 GARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGD-SDSVYEIKIRPPRGTTVT 724

Query: 731 VLPKKLEFNANKTTQSYEI----SFTKISGFTKSASFGSIQWSDGSHIVRSPIAVS 764
           V P+KL F       S+ +    +  K+S    +   G I WSDG   V SP+ V+
Sbjct: 725 VEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVT 770

BLAST of Spg014889 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 715.3 bits (1845), Expect = 5.3e-206
Identity = 385/771 (49.94%), Postives = 501/771 (64.98%), Query Frame = 0

Query: 6   LCSSVFLFFFILCFVPVIFSRSPEDQQTFIVHVSKSEKPSLFSSHHHWHSSILESLSPSP 65
           + SS  +    L F P I   + +  +TFI  +     PS+F +H+HW+S+     S   
Sbjct: 1   MASSTIVLLLFLSF-PFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAEES--- 60

Query: 66  HPTKLLYNYERAANGFSAHITAAQAAELRQVPGVISVIPDRIRQLHTTRTPHFLGLADNL 125
              ++++ Y    +GFSA +T  +A  LR  P V++V  DR R+LHTTR+P FLGL +  
Sbjct: 61  ---RIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQK 120

Query: 126 GLWPDANYAEDVIIGVLDTGIWPERPSFSDEGLSPVPESWKGICETGDRFSASACNRKII 185
           GLW +++Y  DVIIGV DTGIWPER SFSD  L P+P+ W+G+CE+G RFS   CNRKII
Sbjct: 121 GLWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKII 180

Query: 186 GARAYFYGYESNLGRSLQDSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARG 245
           GAR +  G ++ +   +  + +F S RD DGHGTHT+STAAG     AS   YA G A+G
Sbjct: 181 GARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKG 240

Query: 246 MASKARIAAYKICW-EFGCFDSDILAAMDQAIADGVHIISLSV-GSTGRAPAYYRDSIAI 305
           +A KARIAAYK+CW + GC DSDILAA D A+ DGV +IS+S+ G  G    YY D IAI
Sbjct: 241 VAPKARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAI 300

Query: 306 GAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFSGV 365
           G++GA   G+ VS SAGN GP   +  N+APW+ TVGASTIDR F AD ILGDG    GV
Sbjct: 301 GSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGV 360

Query: 366 SLYSGDPLGDSKLPLVYAGDCG---DRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSSV 425
           SLY+G PL     P+VY G  G      C   TLD  +V GKIV+CDRG + RVAKG  V
Sbjct: 361 SLYAGVPLNGRMFPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVV 420

Query: 426 KTAGGLGMILANTEENGEELLADSHLIPGTMVGEIAGNKLKEYIHSDPNPTATIVFRGTV 485
           K AGG+GMILAN   NGE L+ D+HLIP   VG   G+++K Y  S PNP A+I FRGT+
Sbjct: 421 KKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTI 480

Query: 486 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFN 545
           +G   PAP +A+FS RGPN  + EILKPD+IAPGVNILA W+    PT L  DPR+ EFN
Sbjct: 481 VG-IKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFN 540

Query: 546 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDTSGSPIKDLSTSKESNP 605
           I+SGTSM+CPHVSG AALL+ A P WSPA I+SA++TT+  +D S   + D ST K + P
Sbjct: 541 ILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATP 600

Query: 606 FVHGAGHIDPNIALNPGLIYDINPQDYVSFLCSIGYDSKQIAVFVKESSYAKLCEHKLSN 665
           + +G+GH++   A+NPGL+YDI   DY++FLCSIGY  K I V  +  +  +    +  +
Sbjct: 601 YDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITR--TPVRCPTTRKPS 660

Query: 666 PGNLNYPSFAVVFDSDAGEVVKYT--RTVTNVGDEADAVYEVKVEAPEGVEISVLPKKLE 725
           PGNLNYPS   VF ++   +V  T  RT TNVG +A+AVY  ++E+P GV ++V P +L 
Sbjct: 661 PGNLNYPSITAVFPTNRRGLVSKTVIRTATNVG-QAEAVYRARIESPRGVTVTVKPPRLV 720

Query: 726 FNANKTTQSYEISFT-----KISGFTKSASFGSIQWSD-GSHIVRSPIAVS 764
           F +    +SY ++ T      + G T  A FGS+ W D G H+VRSPI V+
Sbjct: 721 FTSAVKRRSYAVTVTVNTRNVVLGET-GAVFGSVTWFDGGKHVVRSPIVVT 759