Spg014794 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg014794 vs. NCBI nr
Match: XP_008461332.2 (PREDICTED: disease resistance protein RGA2 [Cucumis melo])

HSP 1 Score: 471.1 bits (1211), Expect = 1.8e-128
Identity = 275/479 (57.41%), Postives = 328/479 (68.48%), Query Frame = 0

Query: 216  ADDVLDELSGEALHREVMTRGKNAKQELCSRFFYENSSDEYNFDDCVHMHDVIRELARKV 275
            A   ++   GE   + + T GKN   ELC RFFYENSSDE NFDD V+MHDV+ ELARKV
Sbjct: 711  AQGFIESSKGE---KSMETIGKNYFDELCWRFFYENSSDENNFDDYVYMHDVMYELARKV 770

Query: 276  AGKKLYVRGNLNNDYVVSEQTRHISFDYVIISWRDVLSKLRKAKGLRTFLLLHSYYANEN 335
            A KKLYV G++NN+YVVSEQT H SFDY I SW+DVLSKL KAKGLRTF+ LHS Y  +N
Sbjct: 771  ADKKLYVCGDINNNYVVSEQTLHFSFDYKIQSWQDVLSKLCKAKGLRTFMFLHSPYEEKN 830

Query: 336  KVNKTILDELFSSFSHLRVLSLYASNIYEVPNSIRKLRQLRYLDLSENCRMELLPNSITR 395
            +VN+  LDELFSSF  LRVL L  SNI+ +PNSI+KL+ LRYLDLSEN  M+ LPNSIT 
Sbjct: 831  EVNEANLDELFSSFPRLRVLLLGGSNIHVLPNSIKKLKLLRYLDLSEN-NMKSLPNSITE 890

Query: 396  LQNLQTLKLTSCSGLKELPRDTSNLVNLRHLDFP-SKKIRHM-PKGMEKLTCLQRITLFE 455
            LQ+LQTLKLT C  LKE+PRD + LVNLRHLD     +I HM P+GMEKL+CLQ I+LF 
Sbjct: 891  LQDLQTLKLTGCYALKEMPRDINKLVNLRHLDSRCCSQITHMAPEGMEKLSCLQTISLFV 950

Query: 456  LDCKRFSKPSELNNFNNLSTQSWRIIGLEQLRSSPPEVSSVNPKGGKDLRDLTLEWKLSQ 515
             DC + +K  ELN  NNL T   +I GL+QLRS+P EVS VN K  K LR L LEWK+  
Sbjct: 951  FDCTKTNKLRELNELNNL-TGELKITGLQQLRSTPYEVSLVNLKDKKCLRSLKLEWKMGL 1010

Query: 516  EECEAASDERIMEGLEPHPNVEILRIDG------------------------C------- 575
             + E  +DER+M+GLEP+PNVE L I G                        C       
Sbjct: 1011 YKHEDEADERVMKGLEPNPNVESLGIKGYNGVGLPKWMFNCHLKLTEIEIVSCHRLQHLP 1070

Query: 576  ----LRALKFLYLKDLRCLKFIDKND-PYSSSVSFPSLEKLYLEDISNLEGWWESGESRT 635
                L+ LK L L+DLR LKFIDK D P+SSS+ FPSL+ L L +++NLEGWWE   +  
Sbjct: 1071 QFHHLQTLKTLCLRDLRSLKFIDKFDNPFSSSMFFPSLKSLCLANMANLEGWWELSRAVA 1130

Query: 636  GETEK--EGLPPTFPQLQFLYIYHCPKLTSMPKLP-STRALVTLCDVGVQMDELTNGTV 654
            GET +  + LPPTFP+L+ L IY CPKL SMPKLP ST+A V+LCDVGVQ+   T G V
Sbjct: 1131 GETSENIQWLPPTFPELEILDIYQCPKLNSMPKLPSSTKAYVSLCDVGVQLVISTIGPV 1184

BLAST of Spg014794 vs. NCBI nr
Match: XP_008461332.2 (PREDICTED: disease resistance protein RGA2 [Cucumis melo])

HSP 1 Score: 109.8 bits (273), Expect = 1.1e-19
Identity = 58/113 (51.33%), Postives = 78/113 (69.03%), Query Frame = 0

Query: 141 MAEGVLFTVAGEILMKLTSHASQRLGMLCGFEDDLNKLKSNLSAIQAVLLDAEEQPTKSH 200
           MAEG+L  +AGEIL KL S  S+  GML G +DDL++LKS +S IQ+ L+DA+  PT   
Sbjct: 255 MAEGILLQLAGEILTKLISLLSEEYGMLSGLKDDLDQLKSTVSMIQSTLIDAQNLPTGLD 314

Query: 201 SLKDWLEKLGDALYDADDVLDELSGEALHREVMTRGKNAKQELCSRFFYENSS 254
           S+  WLEKL   L+DA+D++DE   E  HREVMTR K+AK+    R F+ +S+
Sbjct: 315 SVATWLEKLRHVLHDAEDLVDEFCAELRHREVMTREKHAKK---VRIFFSSSN 364

HSP 2 Score: 469.9 bits (1208), Expect = 4.1e-128
Identity = 274/479 (57.20%), Postives = 328/479 (68.48%), Query Frame = 0

Query: 216 ADDVLDELSGEALHREVMTRGKNAKQELCSRFFYENSSDEYNFDDCVHMHDVIRELARKV 275
           A   ++   GE   + + T GKN   ELC RFFYENSSDE NFDD V+MHDV+ ELARKV
Sbjct: 458 AQGFIESSKGE---KSMETIGKNYFDELCWRFFYENSSDENNFDDYVYMHDVMYELARKV 517

Query: 276 AGKKLYVRGNLNNDYVVSEQTRHISFDYVIISWRDVLSKLRKAKGLRTFLLLHSYYANEN 335
           A KKL+V G++NN+YVVSEQT H S DY I SW+DVLSKL KAKGLRTF+ LHS Y  +N
Sbjct: 518 ADKKLFVCGDINNNYVVSEQTLHFSLDYKIQSWQDVLSKLCKAKGLRTFMFLHSPYEEKN 577

Query: 336 KVNKTILDELFSSFSHLRVLSLYASNIYEVPNSIRKLRQLRYLDLSENCRMELLPNSITR 395
           +VN+  LDELFSSF  LRVL L  SNI+ +PNSI+KL+ LRYLDLSEN  M+ LPNSIT 
Sbjct: 578 EVNEANLDELFSSFPRLRVLLLGGSNIHVLPNSIKKLKLLRYLDLSEN-NMKSLPNSITE 637

Query: 396 LQNLQTLKLTSCSGLKELPRDTSNLVNLRHLDFP-SKKIRHM-PKGMEKLTCLQRITLFE 455
           LQ+LQTLKLT C  LKE+PRD + LVNLRHLD     +I HM P+GMEKL+CLQ I+LF 
Sbjct: 638 LQDLQTLKLTGCYALKEMPRDINKLVNLRHLDSRCCSQITHMAPEGMEKLSCLQTISLFV 697

Query: 456 LDCKRFSKPSELNNFNNLSTQSWRIIGLEQLRSSPPEVSSVNPKGGKDLRDLTLEWKLSQ 515
            DC + +K  ELN  NNL T   +I GL+QLRS+P EVS VN KG K LR L LEWK+  
Sbjct: 698 FDCTKTNKLRELNELNNL-TGELKITGLQQLRSTPYEVSLVNLKGKKCLRSLKLEWKMGL 757

Query: 516 EECEAASDERIMEGLEPHPNVEILRIDG------------------------C------- 575
            + E  +DER+M+GLEP+PNVE L I G                        C       
Sbjct: 758 YKHEDEADERVMKGLEPNPNVESLGIKGYNGVGLPKWMFNCHLKLTEIEMVSCHRLQHLP 817

Query: 576 ----LRALKFLYLKDLRCLKFIDKND-PYSSSVSFPSLEKLYLEDISNLEGWWESGESRT 635
               L+ LK L L+DLR LKFIDK D P+SSS+ FPSL+ L L +++NLEGWWE   +  
Sbjct: 818 QFHHLQTLKTLCLRDLRSLKFIDKFDNPFSSSMFFPSLKSLCLANMANLEGWWELSRAVA 877

Query: 636 GETEK--EGLPPTFPQLQFLYIYHCPKLTSMPKLP-STRALVTLCDVGVQMDELTNGTV 654
           GET +  + LPPTFP+L+ L IY CPKL SMPKLP ST+A V+LCDVGVQ+   T G V
Sbjct: 878 GETSENIQWLPPTFPELEILDIYQCPKLNSMPKLPSSTKAYVSLCDVGVQLVISTIGPV 931

BLAST of Spg014794 vs. NCBI nr
Match: KAA0058606.1 (disease resistance protein RGA2 [Cucumis melo var. makuwa])

HSP 1 Score: 109.8 bits (273), Expect = 1.1e-19
Identity = 58/113 (51.33%), Postives = 78/113 (69.03%), Query Frame = 0

Query: 141 MAEGVLFTVAGEILMKLTSHASQRLGMLCGFEDDLNKLKSNLSAIQAVLLDAEEQPTKSH 200
           MAEG+L  +AGEIL KL S  S+  GML G +DDL++LKS +S IQ+ L+DA+  PT   
Sbjct: 1   MAEGILLQLAGEILTKLISLLSEEYGMLSGLKDDLDQLKSTVSMIQSTLIDAQNWPTGLD 60

Query: 201 SLKDWLEKLGDALYDADDVLDELSGEALHREVMTRGKNAKQELCSRFFYENSS 254
           S+  WLEKL   L+DA+D++DE   E  HREVMTR K+AK+    R F+ +S+
Sbjct: 61  SVATWLEKLRHVLHDAEDLVDEFCAELRHREVMTREKHAKK---VRIFFSSSN 110

HSP 2 Score: 463.8 bits (1192), Expect = 2.9e-126
Identity = 274/475 (57.68%), Postives = 315/475 (66.32%), Query Frame = 0

Query: 236 GKNAKQELCSRFFYENSSDEYNFDDCVHMHDVIRELARKVAGKKLYVRGNLNNDYVVSEQ 295
           GK+   ELC RFFY NSSDE N +D V MHDV+ E  RKVAG KLYVRGN NNDYVVSEQ
Sbjct: 471 GKDYFNELCWRFFYANSSDECNINDIVCMHDVMCEFVRKVAGNKLYVRGNPNNDYVVSEQ 530

Query: 296 TRHISFDYVIISWRDVLSKLRKAKGLRTFLLLHSYYANENKVNKTILDELFSSFSHLRVL 355
           T HISFDY I SW+DVLSKL KAKGLRT LLL   Y   NK++K ILDELFSSF  LRVL
Sbjct: 531 TLHISFDYGIQSWQDVLSKLCKAKGLRTILLLFRPYEKMNKIDKAILDELFSSFPRLRVL 590

Query: 356 SLYASNIYEVPNSIRKLRQLRYLDLSENCRMELLPNSITRLQNLQTLKLTSCSGLKELPR 415
            L+ S I  VP SI+KLR LRYLDLSEN  MEL+P+SI  LQNLQTL LT C  LKELPR
Sbjct: 591 DLHFSQISVVPKSIKKLRHLRYLDLSEN-DMELIPHSIIELQNLQTLNLTECYELKELPR 650

Query: 416 DTSNLVNLRHLDF-PSKKIRHMPKGMEKLTCLQRITLFELDCKRFSKPSELNNFNNLSTQ 475
           D  NLVNLRHL F P  ++    +GMEKLTCLQ I+LF  DCK+ +K  ELN+ + L T 
Sbjct: 651 DIDNLVNLRHLTFEPCMEVTPTSEGMEKLTCLQTISLFVFDCKKTNKLWELNDLSYL-TG 710

Query: 476 SWRIIGLEQLRSSPPEVSSVNPKGGKDLRDLTLEWKLSQEECEAASDERIMEGLEPHPNV 535
             +IIGLE+LRSSP E++ +N K  K  + L LEWKL ++E E  +DE IMEGLEPHPNV
Sbjct: 711 ELKIIGLEKLRSSPSEITLINLKDKKGWQGLNLEWKLGKDEYEGEADETIMEGLEPHPNV 770

Query: 536 EILRIDG------------------------C-----------LRALKFLYLKDLRCLKF 595
           E L I+G                        C           L+ L+ L+L  LR L+F
Sbjct: 771 ESLSINGYTGGALPNWVFNSLMKLTEIEIENCPRVQHLPQFNQLQDLRALHLVGLRSLEF 830

Query: 596 IDKNDPYSSSVSFPSLEKLYLEDISNLEGWWESGESRTGETEKEG----LPPTFPQLQFL 655
           IDK+DPYSSSV FPSL+ L LED+ NLEGWWE GES+    E  G    LPPTFPQ+ FL
Sbjct: 831 IDKSDPYSSSVFFPSLKFLRLEDMPNLEGWWELGESKVVARETSGKAKWLPPTFPQVNFL 890

Query: 656 YIYHCPKLTSMPKLPSTRALVTLCDVGVQMDELTNGTVSRTHML--FGMINCQLI 669
            IY CPKL+SMPKL S  A V L D+GVQM   T G VS    L   GM N + +
Sbjct: 891 RIYGCPKLSSMPKLASIGADVILHDIGVQMVS-TIGPVSSFMFLSMHGMTNLKYL 942

BLAST of Spg014794 vs. NCBI nr
Match: XP_031745107.1 (putative disease resistance protein RGA4 [Cucumis sativus])

HSP 1 Score: 131.7 bits (330), Expect = 2.6e-26
Identity = 82/168 (48.81%), Postives = 112/168 (66.67%), Query Frame = 0

Query: 141 MAEGVLFTVAGEILMKLTSHASQRLGMLCGFEDDLNKLKSNLSAIQAVLLDAEEQPTKSH 200
           MAE +LF VAGEILMKL+S A QRLGML G + DLNKL + +S I+ VLLDAE + TKSH
Sbjct: 1   MAEAILFQVAGEILMKLSSQAFQRLGMLFGLKGDLNKLTTTVSTIKDVLLDAEGRQTKSH 60

Query: 201 SLKDWLEKLGDALYDADDVLDELSGEALHREVMTRG-KNAKQELCSRFFYENSSDEYNFD 260
            L++WL KL +ALYDA+DVLDELS EAL RE+MTR  KNAKQ     FF +++   +N+ 
Sbjct: 61  LLQNWLHKLEEALYDAEDVLDELSTEALRRELMTRDHKNAKQ--VRIFFSKSNQIAFNYR 120

Query: 261 DCVHMHDVIRELARKVAGK-KLYVRGNLNNDYVVSEQTRHISFDYVII 307
               + ++   L    A K + ++R N  +      +T++ SFD +++
Sbjct: 121 MARQIKNIWERLDAIDAEKTQFHLRENCES------RTQYGSFDRIMM 160

HSP 2 Score: 463.8 bits (1192), Expect = 2.9e-126
Identity = 274/475 (57.68%), Postives = 315/475 (66.32%), Query Frame = 0

Query: 236 GKNAKQELCSRFFYENSSDEYNFDDCVHMHDVIRELARKVAGKKLYVRGNLNNDYVVSEQ 295
           GK+   ELC RFFY NSSDE N +D V MHDV+ E  RKVAG KLYVRGN NNDYVVSEQ
Sbjct: 471 GKDYFNELCWRFFYANSSDECNINDIVCMHDVMCEFVRKVAGNKLYVRGNPNNDYVVSEQ 530

Query: 296 TRHISFDYVIISWRDVLSKLRKAKGLRTFLLLHSYYANENKVNKTILDELFSSFSHLRVL 355
           T HISFDY I SW+DVLSKL KAKGLRT LLL   Y   NK++K ILDELFSSF  LRVL
Sbjct: 531 TLHISFDYGIQSWQDVLSKLCKAKGLRTILLLFRPYEKMNKIDKAILDELFSSFPRLRVL 590

Query: 356 SLYASNIYEVPNSIRKLRQLRYLDLSENCRMELLPNSITRLQNLQTLKLTSCSGLKELPR 415
            L+ S I  VP SI+KLR LRYLDLSEN  MEL+P+SI  LQNLQTL LT C  LKELPR
Sbjct: 591 DLHFSQISVVPKSIKKLRHLRYLDLSEN-DMELIPHSIIELQNLQTLNLTECYELKELPR 650

Query: 416 DTSNLVNLRHLDF-PSKKIRHMPKGMEKLTCLQRITLFELDCKRFSKPSELNNFNNLSTQ 475
           D  NLVNLRHL F P  ++    +GMEKLTCLQ I+LF  DCK+ +K  ELN+ + L T 
Sbjct: 651 DIDNLVNLRHLTFEPCMEVTPTSEGMEKLTCLQTISLFVFDCKKTNKLWELNDLSYL-TG 710

Query: 476 SWRIIGLEQLRSSPPEVSSVNPKGGKDLRDLTLEWKLSQEECEAASDERIMEGLEPHPNV 535
             +IIGLE+LRSSP E++ +N K  K  + L LEWKL ++E E  +DE IMEGLEPHPNV
Sbjct: 711 ELKIIGLEKLRSSPSEITLINLKDKKGWQGLNLEWKLGKDEYEGEADETIMEGLEPHPNV 770

Query: 536 EILRIDG------------------------C-----------LRALKFLYLKDLRCLKF 595
           E L I+G                        C           L+ L+ L+L  LR L+F
Sbjct: 771 ESLSINGYTGGALPNWVFNSLMKLTEIEIENCPRVQHLPQFNQLQDLRALHLVGLRSLEF 830

Query: 596 IDKNDPYSSSVSFPSLEKLYLEDISNLEGWWESGESRTGETEKEG----LPPTFPQLQFL 655
           IDK+DPYSSSV FPSL+ L LED+ NLEGWWE GES+    E  G    LPPTFPQ+ FL
Sbjct: 831 IDKSDPYSSSVFFPSLKFLRLEDMPNLEGWWELGESKVVARETSGKAKWLPPTFPQVNFL 890

Query: 656 YIYHCPKLTSMPKLPSTRALVTLCDVGVQMDELTNGTVSRTHML--FGMINCQLI 669
            IY CPKL+SMPKL S  A V L D+GVQM   T G VS    L   GM N + +
Sbjct: 891 RIYGCPKLSSMPKLASIGADVILHDIGVQMVS-TIGPVSSFMFLSMHGMTNLKYL 942

BLAST of Spg014794 vs. NCBI nr
Match: KAE8646395.1 (hypothetical protein Csa_016171 [Cucumis sativus])

HSP 1 Score: 131.7 bits (330), Expect = 2.6e-26
Identity = 82/168 (48.81%), Postives = 112/168 (66.67%), Query Frame = 0

Query: 141 MAEGVLFTVAGEILMKLTSHASQRLGMLCGFEDDLNKLKSNLSAIQAVLLDAEEQPTKSH 200
           MAE +LF VAGEILMKL+S A QRLGML G + DLNKL + +S I+ VLLDAE + TKSH
Sbjct: 1   MAEAILFQVAGEILMKLSSQAFQRLGMLFGLKGDLNKLTTTVSTIKDVLLDAEGRQTKSH 60

Query: 201 SLKDWLEKLGDALYDADDVLDELSGEALHREVMTRG-KNAKQELCSRFFYENSSDEYNFD 260
            L++WL KL +ALYDA+DVLDELS EAL RE+MTR  KNAKQ     FF +++   +N+ 
Sbjct: 61  LLQNWLHKLEEALYDAEDVLDELSTEALRRELMTRDHKNAKQ--VRIFFSKSNQIAFNYR 120

Query: 261 DCVHMHDVIRELARKVAGK-KLYVRGNLNNDYVVSEQTRHISFDYVII 307
               + ++   L    A K + ++R N  +      +T++ SFD +++
Sbjct: 121 MARQIKNIWERLDAIDAEKTQFHLRENCES------RTQYGSFDRIMM 160

HSP 2 Score: 424.5 bits (1090), Expect = 2.0e-114
Identity = 256/451 (56.76%), Postives = 299/451 (66.30%), Query Frame = 0

Query: 236 GKNAKQELCSRFFYENSSDEYNFDDCVHMHDVIRELARKVAGKKLYVRGNLNNDYVVSEQ 295
           GK   +ELC RFF ENS +E NFD+ VHMHDV+R+LA  VAGKK YVR N+N DY  SE+
Sbjct: 470 GKGYFKELCWRFFLENSMNECNFDNDVHMHDVMRDLATNVAGKK-YVRENINCDYAFSEK 529

Query: 296 TRHISFDYVIISWRDVLSKLRKAKGLRTFLLLHS--YYANENKVNKTILDELFSSFSHLR 355
           TRH+SFD  I SW DVLSKL KAKGLRTF L  S   Y  +N++N+ ILDE+FSSF  LR
Sbjct: 530 TRHVSFDCKIKSWSDVLSKLHKAKGLRTFQLFSSNFEYEKKNEINEAILDEVFSSFPRLR 589

Query: 356 VLSLYASNIYEVPNSIRKLRQLRYLDLSENCRMELLPNSITRLQNLQTLKLTSCSGLKEL 415
           VL L  SN   VPNSIR+LR LRYLDLSEN  ME LPNSIT L+NLQTL L  C  LKEL
Sbjct: 590 VLGLNNSNSPMVPNSIRRLRHLRYLDLSEN-NMESLPNSITELRNLQTLNLMYCGDLKEL 649

Query: 416 PRDTSNLVNLRHLDFPSKKIRHMPKGMEKLTCLQRITLFELDCKRFSKPSELNNFNNLST 475
           PRDTSNLVNLRHLDF +  + HMP+GM KL+CLQ ++ F LDCKR ++ SEL+   +L+ 
Sbjct: 650 PRDTSNLVNLRHLDFNTSILTHMPEGMGKLSCLQTLSYFVLDCKRSNQLSELDVLIHLNG 709

Query: 476 QSWRIIGLEQLRSSPPEVSSVNPKGGKDLRDLTLEWKLS--QEECEAASDE-RIMEGLEP 535
              RIIGLEQLRS   EVS VN K  K L+ L LEWKLS   +E E  +DE  IMEGLEP
Sbjct: 710 Y-LRIIGLEQLRSE-SEVSLVNLKDIKGLKKLELEWKLSGDDQEYEGENDETAIMEGLEP 769

Query: 536 HPNVEILRIDG-------------------------C-----------LRALKFLYLKDL 595
           HPN+E L IDG                         C           L+AL FLYL D+
Sbjct: 770 HPNIESLHIDGYSGVGLPNWVVSTSLSKLTNIVIHKCHRLQHLSQMGHLQALTFLYLDDM 829

Query: 596 RCLKFIDKNDPYSSSVSFPSLEKLYLEDISNLEGWWESGESRTGETEKEGLPPTFPQLQF 646
           R LKFIDKN+P S S SFPSL+ L +E++ NLEGWWE      G T+K  LP TFP+L  
Sbjct: 830 RSLKFIDKNEP-SFSSSFPSLKLLIIENMPNLEGWWE-----LGNTQKNWLPLTFPKLIS 889

BLAST of Spg014794 vs. ExPASy Swiss-Prot
Match: Q9LRR4 (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RPPL1 PE=3 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 2.6e-29
Identity = 130/458 (28.38%), Postives = 206/458 (44.98%), Query Frame = 0

Query: 234 TRGKNAKQELCSRFFYENSSDE--YNFDDCVHMHDVIRELARKVAGKKLYVRGNLNNDYV 293
           TR     +EL + +F E  S            MHD I ELA+  +G +   +        
Sbjct: 460 TRSSKNLEELGNEYFSELESRSLLQKTKTRYIMHDFINELAQFASG-EFSSKFEDGCKLQ 519

Query: 294 VSEQTRHISFDYVIISWRDVLSKLRKAKGLRTFLLLHSYYANEN-KVNKTILDELFSSFS 353
           VSE+TR++S+     +       LR+ K LRTFL L    ++ +  +++ + ++L  + +
Sbjct: 520 VSERTRYLSYLRDNYAEPMEFEALREVKFLRTFLPLSLTNSSRSCCLDQMVSEKLLPTLT 579

Query: 354 HLRVLSLYASNIYEV-PNSIRKLRQLRYLDLSENCRMELLPNSITRLQNLQTLKLTSCSG 413
            LRVLSL    I  + P+  + +   R+LDLS    +E LP S+  + NLQTL L+ CS 
Sbjct: 580 RLRVLSLSHYKIARLPPDFFKNISHARFLDLSRT-ELEKLPKSLCYMYNLQTLLLSYCSS 639

Query: 414 LKELPRDTSNLVNLRHLDFPSKKIRHMPKGMEKLTCLQRITLFELDCKRFSKPSELNNFN 473
           LKELP D SNL+NLR+LD    K+R MP+   +L  LQ +T F +     S+ SEL   +
Sbjct: 640 LKELPTDISNLINLRYLDLIGTKLRQMPRRFGRLKSLQTLTTFFVSASDGSRISELGGLH 699

Query: 474 NLSTQSWRIIGLEQLRSSPPEVSSVNPKGGKDLRDLTLEWKLSQEECE-------AASDE 533
           +L  +  +I+ L+++     + +  N    K LR++   W+      E         ++ 
Sbjct: 700 DLHGK-LKIVELQRV-VDVADAAEANLNSKKHLREIDFVWRTGSSSSENNTNPHRTQNEA 759

Query: 534 RIMEGLEPHPNVEILRIDG-------------------CLRALKFLY------LKDLRCL 593
            + E L PH ++E L I+                    C+R  +  Y      L  L CL
Sbjct: 760 EVFEKLRPHRHIEKLAIERYKGRRFPDWLSDPSFSRIVCIRLRECQYCTSLPSLGQLPCL 819

Query: 594 K------------------FIDKNDPYSSSVSFPSLEKLYLEDISNLEGWWESGESRTGE 636
           K                  F D+         F SLE L  +++ + + W +   +R   
Sbjct: 820 KELHISGMVGLQSIGRKFYFSDQQLRDQDQQPFRSLETLRFDNLPDWQEWLDVRVTR--- 879

HSP 2 Score: 48.1 bits (113), Expect = 5.0e-04
Identity = 23/61 (37.70%), Postives = 39/61 (63.93%), Query Frame = 0

Query: 172 EDDLNKLKSNLSAIQAVLLDAEEQPTKSHSLKDWLEKLGDALYDADDVLDELSGEALHRE 231
           E+ L +L + L  I AVL+DAEE+   +  ++ W+ +L D +Y A+D LD+++ EAL   
Sbjct: 36  ENLLERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLN 95

Query: 232 V 233
           +
Sbjct: 96  I 96

BLAST of Spg014794 vs. ExPASy Swiss-Prot
Match: Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)

HSP 1 Score: 118.2 bits (295), Expect = 4.0e-25
Identity = 112/384 (29.17%), Postives = 168/384 (43.75%), Query Frame = 0

Query: 345 LFSSFSHLRVLSLYASNIYEVPNSIRKLRQLRYLDLSENCRMELLPNSITRLQNLQTLKL 404
           L   F  LRVL+L  SN+ ++P+SI  L  LRYLDLS N R+  LP  + +LQNLQTL L
Sbjct: 520 LLQKFVSLRVLNLRNSNLNQLPSSIGDLVHLRYLDLSGNFRIRNLPKRLCKLQNLQTLDL 579

Query: 405 TSCSGLKELPRDTSNLVNLRHLDFPSKKIRHMPKGMEKLTCLQRITLFELDCKRFSKPSE 464
             C  L  LP+ TS L +LR+L      +   P  +  LTCL+ ++ F +  ++  +  E
Sbjct: 580 HYCDSLSCLPKQTSKLGSLRNLLLDGCSLTSTPPRIGLLTCLKSLSCFVIGKRKGHQLGE 639

Query: 465 LNNFNNLSTQSWRIIGLEQLRSSPPEVSSVNPKGGKDLRDLTLEWKLSQEECEAASDERI 524
           L N N   + S  I  L++++    +    N     +L  L L W L  +      D  +
Sbjct: 640 LKNLNLYGSIS--ITKLDRVKKD-TDAKEANLSAKANLHSLCLSWDLDGKH---RYDSEV 699

Query: 525 MEGLEPHPNVEIL-------------------------RIDGCLRALKFLYLKDLRCLKF 584
           +E L+PH N++ L                         RI GC          +L CL+ 
Sbjct: 700 LEALKPHSNLKYLEINGFGGIRLPDWMNQSVLKNVVSIRIRGCENCSCLPPFGELPCLES 759

Query: 585 IDKNDPYSSSVS----------FPSLEKLYLEDISNLEGWWESGESRTGETEKEGLPPTF 644
           ++ +   S+ V           FPSL KL + D SNL+G  +       E EK+     F
Sbjct: 760 LELHTG-SADVEYVEDNVHPGRFPSLRKLVIWDFSNLKGLLKM------EGEKQ-----F 819

Query: 645 PQLQFLYIYHCPKLTSMPKLPSTRALVTLCDVGVQMDELTNGTVSRTHMLFGMINCQLIS 694
           P L+ +  Y CP    +P L S + L  +      +  ++N  +     L    N +  S
Sbjct: 820 PVLEEMTFYWCPMFV-IPTLSSVKTLKVIVTDATVLRSISN--LRALTSLDISDNVEATS 879

HSP 2 Score: 65.5 bits (158), Expect = 3.0e-09
Identity = 33/75 (44.00%), Postives = 46/75 (61.33%), Query Frame = 0

Query: 153 ILMKLTSHASQRLGMLCGFEDDLNKLKSNLSAIQAVLLDAEEQPTKSHSLKDWLEKLGDA 212
           +L  LTS     L +L GF+D+  +L S  S IQAVL DA+E+      L++WL+KL  A
Sbjct: 9   VLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNDKPLENWLQKLNAA 68

Query: 213 LYDADDVLDELSGEA 228
            Y+ DD+LDE   +A
Sbjct: 69  TYEVDDILDEYKTKA 83

BLAST of Spg014794 vs. ExPASy Swiss-Prot
Match: Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 2.6e-24
Identity = 105/369 (28.46%), Postives = 168/369 (45.53%), Query Frame = 0

Query: 345 LFSSFSHLRVLSLYASNIYEVPNSIRKLRQLRYLDLSENCRMELLPNSITRLQNLQTLKL 404
           L   F  LRVL+L  S + ++P+SI  L  LRYLDLS N     LP  + +LQNLQTL +
Sbjct: 522 LLKKFVSLRVLNLSYSKLEQLPSSIGDLLHLRYLDLSCN-NFRSLPERLCKLQNLQTLDV 581

Query: 405 TSCSGLKELPRDTSNLVNLRHLDFPSKKIRHMPKGMEKLTCLQRITLFELDCKRFSKPSE 464
            +C  L  LP+ TS L +LRHL      +   P  +  LTCL+ +  F +  K+  +  E
Sbjct: 582 HNCYSLNCLPKQTSKLSSLRHLVVDGCPLTSTPPRIGLLTCLKTLGFFIVGSKKGYQLGE 641

Query: 465 LNNFNNLSTQSWRIIGLEQLRSSPPEVSSVNPKGGKDLRDLTLEWKLSQEECEAASDERI 524
           L N N   + S  I  LE++++     ++++ K   +L+ L++ W         + + ++
Sbjct: 642 LKNLNLCGSIS--ITHLERVKNDTDAEANLSAKA--NLQSLSMSWDNDGPNRYESKEVKV 701

Query: 525 MEGLEPHPNVEIL-------------------------RIDGCLRALKFLYLKDLRCL-- 584
           +E L+PHPN++ L                         RI  C   L      +L CL  
Sbjct: 702 LEALKPHPNLKYLEIIAFGGFRFPSWINHSVLEKVISVRIKSCKNCLCLPPFGELPCLEN 761

Query: 585 ----------KFIDKND---PYSSSVSFPSLEKLYLEDISNLEGWW-ESGESRTGETEKE 644
                     ++++++D    +S+  SFPSL+KL +    +L+G   E GE +       
Sbjct: 762 LELQNGSAEVEYVEEDDVHSRFSTRRSFPSLKKLRIWFFRSLKGLMKEEGEEK------- 821

Query: 645 GLPPTFPQLQFLYIYHCPKLTSMPKLPSTRALVTLCDVGVQMDELTNGTVSRTHMLFGMI 673
                FP L+ + I +CP L   P L S + L    +   +     +   + T +  G  
Sbjct: 822 -----FPMLEEMAILYCP-LFVFPTLSSVKKLEVHGNTNTRGLSSISNLSTLTSLRIG-A 871

HSP 2 Score: 69.3 bits (168), Expect = 2.1e-10
Identity = 34/79 (43.04%), Postives = 49/79 (62.03%), Query Frame = 0

Query: 153 ILMKLTSHASQRLGMLCGFEDDLNKLKSNLSAIQAVLLDAEEQPTKSHSLKDWLEKLGDA 212
           +L  LTS    +L ++ GFE +  KL S  S IQAVL DA+E+  K  ++++WL+KL  A
Sbjct: 9   LLENLTSFIGDKLVLIFGFEKECEKLSSVFSTIQAVLQDAQEKQLKDKAIENWLQKLNSA 68

Query: 213 LYDADDVLDELSGEALHRE 232
            Y+ DD+L E   EA+  E
Sbjct: 69  AYEVDDILGECKNEAIRFE 87

BLAST of Spg014794 vs. ExPASy Swiss-Prot
Match: Q9LRR5 (Putative disease resistance protein At3g14460 OS=Arabidopsis thaliana OX=3702 GN=At3g14460 PE=3 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 6.3e-23
Identity = 107/379 (28.23%), Postives = 175/379 (46.17%), Query Frame = 0

Query: 264 MHDVIRELARKVAGKKLYVRGNLNNDYV--VSEQTRHISFDYVIISWRDVLSKLRKAKGL 323
           MHD++ +LA+ V+G   +    L +D +  +   TRH SF             +  A+ L
Sbjct: 487 MHDLMNDLAKAVSGDFCF---RLEDDNIPEIPSTTRHFSFSRSQCDASVAFRSICGAEFL 546

Query: 324 RTFLLLHSYYANEN-KVNKTILDELFSSFSHLRVLSLYASNIYEVPNSIRKLRQLRYLDL 383
           RT L  +S  + E+ ++ + +L+ L ++ S LR+LSL    I  +P S++ L+ LRYLDL
Sbjct: 547 RTILPFNSPTSLESLQLTEKVLNPLLNALSGLRILSLSHYQITNLPKSLKGLKLLRYLDL 606

Query: 384 SENCRMELLPNSITRLQNLQTLKLTSCSGLKELPRDTSNLVNLRHLDFPSKKIRHMPKGM 443
           S + +++ LP  +  L NLQTL L++C  L  LP+  + L+NLR LD     +  MP G+
Sbjct: 607 S-STKIKELPEFVCTLCNLQTLLLSNCRDLTSLPKSIAELINLRLLDLVGTPLVEMPPGI 666

Query: 444 EKLTCLQRITLFELDCKRFSKPSELNNFNNLSTQSWRIIGLEQLRSSPPEVSSVNPKGGK 503
           +KL  LQ+++ F +     +   EL   ++L   + RI  L+ + +   E      K   
Sbjct: 667 KKLRSLQKLSNFVIGRLSGAGLHELKELSHL-RGTLRISELQNV-AFASEAKDAGLKRKP 726

Query: 504 DLRDLTLEWKLSQE-------ECEAASDERIMEGLEPHPNVEILRIDGCLRALKFLYLKD 563
            L  L L+W +             A   + ++  LEPHP+++   I+         +L D
Sbjct: 727 FLDGLILKWTVKGSGFVPGSFNALACDQKEVLRMLEPHPHLKTFCIESYQGGAFPKWLGD 786

Query: 564 LRCLKFIDKNDPYSSSVS----------FPSLEKLYLEDISNLEGWWESGESRTGETEKE 623
                F        SS +           PSL+ L +E  + L+      +   GE    
Sbjct: 787 ---SSFFGITSVTLSSCNLCISLPPVGQLPSLKYLSIEKFNILQK--VGLDFFFGENNSR 846

HSP 2 Score: 50.8 bits (120), Expect = 7.8e-05
Identity = 33/96 (34.38%), Postives = 50/96 (52.08%), Query Frame = 0

Query: 141 MAEGVLFTVAGEILMKLTSHASQRLGMLCGFEDD---LNKLKSNLSAIQAVLLDAEEQPT 200
           MA   L + A  ++ ++  + SQ L  LC  +     L +LK  L     VL DA+++  
Sbjct: 1   MANSYLSSCANVMVERI--NTSQELVELCKGKSSSALLKRLKVALVTANPVLADADQRAE 60

Query: 201 KSHSLKDWLEKLGDALYDADDVLDELSGEALHREVM 234
               +K WL  + DA + A+D+LDEL  EAL R V+
Sbjct: 61  HVREVKHWLTGIKDAFFQAEDILDELQTEALRRRVV 94

BLAST of Spg014794 vs. ExPASy Swiss-Prot
Match: Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)

HSP 1 Score: 106.7 bits (265), Expect = 1.2e-21
Identity = 95/327 (29.05%), Postives = 145/327 (44.34%), Query Frame = 0

Query: 345 LFSSFSHLRVLSLYASNIYEVPNSIRKLRQLRYLDLSENCRMELLPNSITRLQNLQTLKL 404
           LF  F  LRVL+L  S   ++P+S+  L  LRYLDLS N ++  LP  + +LQNLQTL L
Sbjct: 530 LFKRFVSLRVLNLSNSEFEQLPSSVGDLVHLRYLDLSGN-KICSLPKRLCKLQNLQTLDL 589

Query: 405 TSCSGLKELPRDTSNLVNLRHLDFPSKKIRHMPKGMEKLTCLQRITLFELDCKRFSKPSE 464
            +C  L  LP+ TS L +LR+L      +  MP  +  LTCL+ +  F +  ++  +  E
Sbjct: 590 YNCQSLSCLPKQTSKLCSLRNLVLDHCPLTSMPPRIGLLTCLKTLGYFVVGERKGYQLGE 649

Query: 465 LNNFNNLSTQSWRIIGLEQLRSSPPEVSSVNPKGGKDLRDLTLEWKLSQEECEAASDERI 524
           L N N     S  I  LE++++   E    N     +L  L++ W   +     + + ++
Sbjct: 650 LRNLNLRGAIS--ITHLERVKND-MEAKEANLSAKANLHSLSMSW--DRPNRYESEEVKV 709

Query: 525 MEGLEPHPNVEILR-------------------------IDGCLRALKFLYLKDLRCLKF 584
           +E L+PHPN++ L                          I GC          +L CL+ 
Sbjct: 710 LEALKPHPNLKYLEIIDFCGFCLPDWMNHSVLKNVVSILISGCENCSCLPPFGELPCLES 769

Query: 585 IDKND-----------PYSSSVSFPSLEKLYLEDISNLEGWWESGESRTGETEKEGLPPT 636
           ++  D            + +   FPSL KL++    NL+G       R    E+      
Sbjct: 770 LELQDGSVEVEYVEDSGFLTRRRFPSLRKLHIGGFCNLKGL-----QRMKGAEQ------ 829

HSP 2 Score: 67.0 bits (162), Expect = 1.0e-09
Identity = 34/75 (45.33%), Postives = 47/75 (62.67%), Query Frame = 0

Query: 153 ILMKLTSHASQRLGMLCGFEDDLNKLKSNLSAIQAVLLDAEEQPTKSHSLKDWLEKLGDA 212
           +L  LT      LG++ GFE +  KL S  S IQAVL DA+E+  K  ++K+WL+KL  A
Sbjct: 9   LLDNLTFFIQGELGLVFGFEKEFKKLSSMFSMIQAVLEDAQEKQLKYKAIKNWLQKLNVA 68

Query: 213 LYDADDVLDELSGEA 228
            Y+ DD+LD+   EA
Sbjct: 69  AYEVDDILDDCKTEA 83

BLAST of Spg014794 vs. ExPASy TrEMBL
Match: A0A1S3CEH4 (disease resistance protein RGA2 OS=Cucumis melo OX=3656 GN=LOC103499949 PE=4 SV=1)

HSP 1 Score: 471.1 bits (1211), Expect = 8.9e-129
Identity = 275/479 (57.41%), Postives = 328/479 (68.48%), Query Frame = 0

Query: 216  ADDVLDELSGEALHREVMTRGKNAKQELCSRFFYENSSDEYNFDDCVHMHDVIRELARKV 275
            A   ++   GE   + + T GKN   ELC RFFYENSSDE NFDD V+MHDV+ ELARKV
Sbjct: 711  AQGFIESSKGE---KSMETIGKNYFDELCWRFFYENSSDENNFDDYVYMHDVMYELARKV 770

Query: 276  AGKKLYVRGNLNNDYVVSEQTRHISFDYVIISWRDVLSKLRKAKGLRTFLLLHSYYANEN 335
            A KKLYV G++NN+YVVSEQT H SFDY I SW+DVLSKL KAKGLRTF+ LHS Y  +N
Sbjct: 771  ADKKLYVCGDINNNYVVSEQTLHFSFDYKIQSWQDVLSKLCKAKGLRTFMFLHSPYEEKN 830

Query: 336  KVNKTILDELFSSFSHLRVLSLYASNIYEVPNSIRKLRQLRYLDLSENCRMELLPNSITR 395
            +VN+  LDELFSSF  LRVL L  SNI+ +PNSI+KL+ LRYLDLSEN  M+ LPNSIT 
Sbjct: 831  EVNEANLDELFSSFPRLRVLLLGGSNIHVLPNSIKKLKLLRYLDLSEN-NMKSLPNSITE 890

Query: 396  LQNLQTLKLTSCSGLKELPRDTSNLVNLRHLDFP-SKKIRHM-PKGMEKLTCLQRITLFE 455
            LQ+LQTLKLT C  LKE+PRD + LVNLRHLD     +I HM P+GMEKL+CLQ I+LF 
Sbjct: 891  LQDLQTLKLTGCYALKEMPRDINKLVNLRHLDSRCCSQITHMAPEGMEKLSCLQTISLFV 950

Query: 456  LDCKRFSKPSELNNFNNLSTQSWRIIGLEQLRSSPPEVSSVNPKGGKDLRDLTLEWKLSQ 515
             DC + +K  ELN  NNL T   +I GL+QLRS+P EVS VN K  K LR L LEWK+  
Sbjct: 951  FDCTKTNKLRELNELNNL-TGELKITGLQQLRSTPYEVSLVNLKDKKCLRSLKLEWKMGL 1010

Query: 516  EECEAASDERIMEGLEPHPNVEILRIDG------------------------C------- 575
             + E  +DER+M+GLEP+PNVE L I G                        C       
Sbjct: 1011 YKHEDEADERVMKGLEPNPNVESLGIKGYNGVGLPKWMFNCHLKLTEIEIVSCHRLQHLP 1070

Query: 576  ----LRALKFLYLKDLRCLKFIDKND-PYSSSVSFPSLEKLYLEDISNLEGWWESGESRT 635
                L+ LK L L+DLR LKFIDK D P+SSS+ FPSL+ L L +++NLEGWWE   +  
Sbjct: 1071 QFHHLQTLKTLCLRDLRSLKFIDKFDNPFSSSMFFPSLKSLCLANMANLEGWWELSRAVA 1130

Query: 636  GETEK--EGLPPTFPQLQFLYIYHCPKLTSMPKLP-STRALVTLCDVGVQMDELTNGTV 654
            GET +  + LPPTFP+L+ L IY CPKL SMPKLP ST+A V+LCDVGVQ+   T G V
Sbjct: 1131 GETSENIQWLPPTFPELEILDIYQCPKLNSMPKLPSSTKAYVSLCDVGVQLVISTIGPV 1184

BLAST of Spg014794 vs. ExPASy TrEMBL
Match: A0A1S3CEH4 (disease resistance protein RGA2 OS=Cucumis melo OX=3656 GN=LOC103499949 PE=4 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 5.2e-20
Identity = 58/113 (51.33%), Postives = 78/113 (69.03%), Query Frame = 0

Query: 141 MAEGVLFTVAGEILMKLTSHASQRLGMLCGFEDDLNKLKSNLSAIQAVLLDAEEQPTKSH 200
           MAEG+L  +AGEIL KL S  S+  GML G +DDL++LKS +S IQ+ L+DA+  PT   
Sbjct: 255 MAEGILLQLAGEILTKLISLLSEEYGMLSGLKDDLDQLKSTVSMIQSTLIDAQNLPTGLD 314

Query: 201 SLKDWLEKLGDALYDADDVLDELSGEALHREVMTRGKNAKQELCSRFFYENSS 254
           S+  WLEKL   L+DA+D++DE   E  HREVMTR K+AK+    R F+ +S+
Sbjct: 315 SVATWLEKLRHVLHDAEDLVDEFCAELRHREVMTREKHAKK---VRIFFSSSN 364

HSP 2 Score: 469.9 bits (1208), Expect = 2.0e-128
Identity = 274/479 (57.20%), Postives = 328/479 (68.48%), Query Frame = 0

Query: 216 ADDVLDELSGEALHREVMTRGKNAKQELCSRFFYENSSDEYNFDDCVHMHDVIRELARKV 275
           A   ++   GE   + + T GKN   ELC RFFYENSSDE NFDD V+MHDV+ ELARKV
Sbjct: 458 AQGFIESSKGE---KSMETIGKNYFDELCWRFFYENSSDENNFDDYVYMHDVMYELARKV 517

Query: 276 AGKKLYVRGNLNNDYVVSEQTRHISFDYVIISWRDVLSKLRKAKGLRTFLLLHSYYANEN 335
           A KKL+V G++NN+YVVSEQT H S DY I SW+DVLSKL KAKGLRTF+ LHS Y  +N
Sbjct: 518 ADKKLFVCGDINNNYVVSEQTLHFSLDYKIQSWQDVLSKLCKAKGLRTFMFLHSPYEEKN 577

Query: 336 KVNKTILDELFSSFSHLRVLSLYASNIYEVPNSIRKLRQLRYLDLSENCRMELLPNSITR 395
           +VN+  LDELFSSF  LRVL L  SNI+ +PNSI+KL+ LRYLDLSEN  M+ LPNSIT 
Sbjct: 578 EVNEANLDELFSSFPRLRVLLLGGSNIHVLPNSIKKLKLLRYLDLSEN-NMKSLPNSITE 637

Query: 396 LQNLQTLKLTSCSGLKELPRDTSNLVNLRHLDFP-SKKIRHM-PKGMEKLTCLQRITLFE 455
           LQ+LQTLKLT C  LKE+PRD + LVNLRHLD     +I HM P+GMEKL+CLQ I+LF 
Sbjct: 638 LQDLQTLKLTGCYALKEMPRDINKLVNLRHLDSRCCSQITHMAPEGMEKLSCLQTISLFV 697

Query: 456 LDCKRFSKPSELNNFNNLSTQSWRIIGLEQLRSSPPEVSSVNPKGGKDLRDLTLEWKLSQ 515
            DC + +K  ELN  NNL T   +I GL+QLRS+P EVS VN KG K LR L LEWK+  
Sbjct: 698 FDCTKTNKLRELNELNNL-TGELKITGLQQLRSTPYEVSLVNLKGKKCLRSLKLEWKMGL 757

Query: 516 EECEAASDERIMEGLEPHPNVEILRIDG------------------------C------- 575
            + E  +DER+M+GLEP+PNVE L I G                        C       
Sbjct: 758 YKHEDEADERVMKGLEPNPNVESLGIKGYNGVGLPKWMFNCHLKLTEIEMVSCHRLQHLP 817

Query: 576 ----LRALKFLYLKDLRCLKFIDKND-PYSSSVSFPSLEKLYLEDISNLEGWWESGESRT 635
               L+ LK L L+DLR LKFIDK D P+SSS+ FPSL+ L L +++NLEGWWE   +  
Sbjct: 818 QFHHLQTLKTLCLRDLRSLKFIDKFDNPFSSSMFFPSLKSLCLANMANLEGWWELSRAVA 877

Query: 636 GETEK--EGLPPTFPQLQFLYIYHCPKLTSMPKLP-STRALVTLCDVGVQMDELTNGTV 654
           GET +  + LPPTFP+L+ L IY CPKL SMPKLP ST+A V+LCDVGVQ+   T G V
Sbjct: 878 GETSENIQWLPPTFPELEILDIYQCPKLNSMPKLPSSTKAYVSLCDVGVQLVISTIGPV 931

BLAST of Spg014794 vs. ExPASy TrEMBL
Match: A0A5A7UWP6 (Disease resistance protein RGA2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G00750 PE=4 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 5.2e-20
Identity = 58/113 (51.33%), Postives = 78/113 (69.03%), Query Frame = 0

Query: 141 MAEGVLFTVAGEILMKLTSHASQRLGMLCGFEDDLNKLKSNLSAIQAVLLDAEEQPTKSH 200
           MAEG+L  +AGEIL KL S  S+  GML G +DDL++LKS +S IQ+ L+DA+  PT   
Sbjct: 1   MAEGILLQLAGEILTKLISLLSEEYGMLSGLKDDLDQLKSTVSMIQSTLIDAQNWPTGLD 60

Query: 201 SLKDWLEKLGDALYDADDVLDELSGEALHREVMTRGKNAKQELCSRFFYENSS 254
           S+  WLEKL   L+DA+D++DE   E  HREVMTR K+AK+    R F+ +S+
Sbjct: 61  SVATWLEKLRHVLHDAEDLVDEFCAELRHREVMTREKHAKK---VRIFFSSSN 110

HSP 2 Score: 463.8 bits (1192), Expect = 1.4e-126
Identity = 274/475 (57.68%), Postives = 315/475 (66.32%), Query Frame = 0

Query: 236 GKNAKQELCSRFFYENSSDEYNFDDCVHMHDVIRELARKVAGKKLYVRGNLNNDYVVSEQ 295
           GK+   ELC RFFY NSSDE N +D V MHDV+ E  RKVAG KLYVRGN NNDYVVSEQ
Sbjct: 88  GKDYFNELCWRFFYANSSDECNINDIVCMHDVMCEFVRKVAGNKLYVRGNPNNDYVVSEQ 147

Query: 296 TRHISFDYVIISWRDVLSKLRKAKGLRTFLLLHSYYANENKVNKTILDELFSSFSHLRVL 355
           T HISFDY I SW+DVLSKL KAKGLRT LLL   Y   NK++K ILDELFSSF  LRVL
Sbjct: 148 TLHISFDYGIQSWQDVLSKLCKAKGLRTILLLFRPYEKMNKIDKAILDELFSSFPRLRVL 207

Query: 356 SLYASNIYEVPNSIRKLRQLRYLDLSENCRMELLPNSITRLQNLQTLKLTSCSGLKELPR 415
            L+ S I  VP SI+KLR LRYLDLSEN  MEL+P+SI  LQNLQTL LT C  LKELPR
Sbjct: 208 DLHFSQISVVPKSIKKLRHLRYLDLSEN-DMELIPHSIIELQNLQTLNLTECYELKELPR 267

Query: 416 DTSNLVNLRHLDF-PSKKIRHMPKGMEKLTCLQRITLFELDCKRFSKPSELNNFNNLSTQ 475
           D  NLVNLRHL F P  ++    +GMEKLTCLQ I+LF  DCK+ +K  ELN+ + L T 
Sbjct: 268 DIDNLVNLRHLTFEPCMEVTPTSEGMEKLTCLQTISLFVFDCKKTNKLWELNDLSYL-TG 327

Query: 476 SWRIIGLEQLRSSPPEVSSVNPKGGKDLRDLTLEWKLSQEECEAASDERIMEGLEPHPNV 535
             +IIGLE+LRSSP E++ +N K  K  + L LEWKL ++E E  +DE IMEGLEPHPNV
Sbjct: 328 ELKIIGLEKLRSSPSEITLINLKDKKGWQGLNLEWKLGKDEYEGEADETIMEGLEPHPNV 387

Query: 536 EILRIDG------------------------C-----------LRALKFLYLKDLRCLKF 595
           E L I+G                        C           L+ L+ L+L  LR L+F
Sbjct: 388 ESLSINGYTGGALPNWVFNSLMKLTEIEIENCPRVQHLPQFNQLQDLRALHLVGLRSLEF 447

Query: 596 IDKNDPYSSSVSFPSLEKLYLEDISNLEGWWESGESRTGETEKEG----LPPTFPQLQFL 655
           IDK+DPYSSSV FPSL+ L LED+ NLEGWWE GES+    E  G    LPPTFPQ+ FL
Sbjct: 448 IDKSDPYSSSVFFPSLKFLRLEDMPNLEGWWELGESKVVARETSGKAKWLPPTFPQVNFL 507

Query: 656 YIYHCPKLTSMPKLPSTRALVTLCDVGVQMDELTNGTVSRTHML--FGMINCQLI 669
            IY CPKL+SMPKL S  A V L D+GVQM   T G VS    L   GM N + +
Sbjct: 508 RIYGCPKLSSMPKLASIGADVILHDIGVQMVS-TIGPVSSFMFLSMHGMTNLKYL 559

BLAST of Spg014794 vs. ExPASy TrEMBL
Match: A0A5D3CEM5 (Putative disease resistance protein RGA3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00620 PE=4 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 2.2e-111
Identity = 251/454 (55.29%), Postives = 294/454 (64.76%), Query Frame = 0

Query: 235 RGKNAKQELCSRFFYENSSDEYNFDDCVHMHDVIRELARKVAGKKLYVRGNLNNDYVVSE 294
           +GK+   ELCSRFFYEN SD+ NFDD V +HDV+RELA+KVAG KLY RG+ NN+YVVSE
Sbjct: 225 KGKDYFDELCSRFFYENLSDDNNFDDYVRIHDVMRELAKKVAGNKLYARGDPNNNYVVSE 284

Query: 295 QTRHISFDYVIISWRDVLSKLRKAKGLRTFLLLHSYYANENKVNKTILDELFSSFSHLRV 354
           QTRHISFDY I  W+DVLSKL KAKGLRTFLLLH  Y  +N ++K  LD+LFSSF  LRV
Sbjct: 285 QTRHISFDYKIELWKDVLSKLHKAKGLRTFLLLHHLYEEKNTLDKQTLDKLFSSFPRLRV 344

Query: 355 LSLYASNIYEVPNSIRKLRQLRYLDLSENCRMELLPNSITRLQNLQTLKLTSCSGLKELP 414
           L L+ S I  VP SI KL  LRYLDLSEN  +E LPNSIT+LQNLQTLKLT C  LK+LP
Sbjct: 345 LGLHDSKISTVPESIEKLTDLRYLDLSEN-DIESLPNSITKLQNLQTLKLTECHKLKKLP 404

Query: 415 RDTSNLVNLRHLDFP-SKKIRHMPKGMEKLTCLQRITLFELDCKRFSKPSELNNFNNLST 474
           RDT  L NLRHL F    +I HMP+GMEKLT LQ ITLF  DCK+ +K SELN  N L  
Sbjct: 405 RDTKELENLRHLAFDHCIQITHMPEGMEKLTSLQTITLFVFDCKKSNKLSELNKLNYLKA 464

Query: 475 QSWRIIGLEQLRSSP---PEVSSVNPKGGKDLRDLTLEWKLS-QEECEAASDERIMEGLE 534
           +  +I GLEQLRS     PEV  VN K G   R L LEW+LS  +E E   D+ IME   
Sbjct: 465 E-LKITGLEQLRSIGSILPEVGLVNLKHG--WRSLKLEWELSGGDEYEGEGDKTIMECFI 524

Query: 535 PHPNVEILRIDG-------------------------C-----------LRALKFLYLKD 594
           PHP +E L I G                         C           L AL  L L++
Sbjct: 525 PHPYLESLSIKGYCGEKLPEWVSAWPFWKLTQIEIVNCHRLKYLPSIKHLHALSNLRLQN 584

Query: 595 LRCLKFIDKNDPYSSSVS--FPSLEKLYLEDISNLEGWWESGESRTGETEKEGLPPTFPQ 646
           LR L+FID +D  +SS S  FPSL+ ++L+D+ NL GW +       +T    LPPTF Q
Sbjct: 585 LRSLEFIDVDDSSNSSSSNFFPSLKFIWLQDLPNLIGWGQ-------DTRTSRLPPTFSQ 644

BLAST of Spg014794 vs. ExPASy TrEMBL
Match: A0A5D3CJL8 (Disease resistance protein RGA2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00520 PE=4 SV=1)

HSP 1 Score: 405.6 bits (1041), Expect = 4.6e-109
Identity = 251/479 (52.40%), Postives = 300/479 (62.63%), Query Frame = 0

Query: 216 ADDVLDELSGEALHREVMTRGKNAKQELCSRFFYENSSDEYNFDDCVHMHDVIRELARKV 275
           A   ++   GE   + + T GKN   ELC RFFYENSSDE NFDD V+MHDV+ ELARK 
Sbjct: 458 AQGFIESSKGE---KSMETIGKNYFDELCWRFFYENSSDENNFDDYVYMHDVMYELARK- 517

Query: 276 AGKKLYVRGNLNNDYVVSEQTRHISFDYVIISWRDVLSKLRKAKGLRTFLLLHSYYANEN 335
                                             DVLSKL KAKGLRTF+ LHS Y  +N
Sbjct: 518 ----------------------------------DVLSKLCKAKGLRTFMFLHSPYEEKN 577

Query: 336 KVNKTILDELFSSFSHLRVLSLYASNIYEVPNSIRKLRQLRYLDLSENCRMELLPNSITR 395
           +VN+  LDELFSSF  LRVL L  SNI+ +PNSI+KL+ LRYLDLSEN  M+ LPNSIT 
Sbjct: 578 EVNEANLDELFSSFPRLRVLLLGGSNIHVLPNSIKKLKLLRYLDLSEN-NMKSLPNSITE 637

Query: 396 LQNLQTLKLTSCSGLKELPRDTSNLVNLRHLDFP-SKKIRHM-PKGMEKLTCLQRITLFE 455
           LQ+LQTLKLT C  LKE+PRD + LVNLRHLD     +I HM P+GMEKL+CLQ I+LF 
Sbjct: 638 LQDLQTLKLTGCYALKEMPRDINKLVNLRHLDSRCCSQITHMAPEGMEKLSCLQTISLFV 697

Query: 456 LDCKRFSKPSELNNFNNLSTQSWRIIGLEQLRSSPPEVSSVNPKGGKDLRDLTLEWKLSQ 515
            DC + +K  ELN  NNL T   +I GL+QLRS+P EVS VN KG K LR L LEWK+  
Sbjct: 698 FDCTKTNKLRELNELNNL-TGELKITGLQQLRSTPYEVSLVNLKGKKCLRSLKLEWKMGL 757

Query: 516 EECEAASDERIMEGLEPHPNVEILRIDG------------------------C------- 575
            + E  +DER+M+GLEP+PNVE L I G                        C       
Sbjct: 758 YKHEDEADERVMKGLEPNPNVESLGIKGYNGVGLPKWMFNCHLKLTEIEIVSCHRLQHLP 817

Query: 576 ----LRALKFLYLKDLRCLKFIDKND-PYSSSVSFPSLEKLYLEDISNLEGWWESGESRT 635
               L+ LK L L+DLR LKFIDK D P+SSS+ FPSL+ L L +++NLEGWWE   +  
Sbjct: 818 QFHHLQTLKTLCLRDLRSLKFIDKFDNPFSSSMFFPSLKSLCLANMANLEGWWELSRAVA 877

Query: 636 GETEK--EGLPPTFPQLQFLYIYHCPKLTSMPKLP-STRALVTLCDVGVQMDELTNGTV 654
           GET +  + LPPTFP+L+ L IY CPKL SMPKLP ST+A V+LCDVGVQ+   T G V
Sbjct: 878 GETSENIQWLPPTFPELEILDIYQCPKLNSMPKLPSSTKAYVSLCDVGVQLVISTIGPV 896

BLAST of Spg014794 vs. TAIR 10
Match: AT3G14470.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 132.1 bits (331), Expect = 1.9e-30
Identity = 130/458 (28.38%), Postives = 206/458 (44.98%), Query Frame = 0

Query: 234 TRGKNAKQELCSRFFYENSSDE--YNFDDCVHMHDVIRELARKVAGKKLYVRGNLNNDYV 293
           TR     +EL + +F E  S            MHD I ELA+  +G +   +        
Sbjct: 460 TRSSKNLEELGNEYFSELESRSLLQKTKTRYIMHDFINELAQFASG-EFSSKFEDGCKLQ 519

Query: 294 VSEQTRHISFDYVIISWRDVLSKLRKAKGLRTFLLLHSYYANEN-KVNKTILDELFSSFS 353
           VSE+TR++S+     +       LR+ K LRTFL L    ++ +  +++ + ++L  + +
Sbjct: 520 VSERTRYLSYLRDNYAEPMEFEALREVKFLRTFLPLSLTNSSRSCCLDQMVSEKLLPTLT 579

Query: 354 HLRVLSLYASNIYEV-PNSIRKLRQLRYLDLSENCRMELLPNSITRLQNLQTLKLTSCSG 413
            LRVLSL    I  + P+  + +   R+LDLS    +E LP S+  + NLQTL L+ CS 
Sbjct: 580 RLRVLSLSHYKIARLPPDFFKNISHARFLDLSRT-ELEKLPKSLCYMYNLQTLLLSYCSS 639

Query: 414 LKELPRDTSNLVNLRHLDFPSKKIRHMPKGMEKLTCLQRITLFELDCKRFSKPSELNNFN 473
           LKELP D SNL+NLR+LD    K+R MP+   +L  LQ +T F +     S+ SEL   +
Sbjct: 640 LKELPTDISNLINLRYLDLIGTKLRQMPRRFGRLKSLQTLTTFFVSASDGSRISELGGLH 699

Query: 474 NLSTQSWRIIGLEQLRSSPPEVSSVNPKGGKDLRDLTLEWKLSQEECE-------AASDE 533
           +L  +  +I+ L+++     + +  N    K LR++   W+      E         ++ 
Sbjct: 700 DLHGK-LKIVELQRV-VDVADAAEANLNSKKHLREIDFVWRTGSSSSENNTNPHRTQNEA 759

Query: 534 RIMEGLEPHPNVEILRIDG-------------------CLRALKFLY------LKDLRCL 593
            + E L PH ++E L I+                    C+R  +  Y      L  L CL
Sbjct: 760 EVFEKLRPHRHIEKLAIERYKGRRFPDWLSDPSFSRIVCIRLRECQYCTSLPSLGQLPCL 819

Query: 594 K------------------FIDKNDPYSSSVSFPSLEKLYLEDISNLEGWWESGESRTGE 636
           K                  F D+         F SLE L  +++ + + W +   +R   
Sbjct: 820 KELHISGMVGLQSIGRKFYFSDQQLRDQDQQPFRSLETLRFDNLPDWQEWLDVRVTR--- 879

HSP 2 Score: 48.1 bits (113), Expect = 3.6e-05
Identity = 23/61 (37.70%), Postives = 39/61 (63.93%), Query Frame = 0

Query: 172 EDDLNKLKSNLSAIQAVLLDAEEQPTKSHSLKDWLEKLGDALYDADDVLDELSGEALHRE 231
           E+ L +L + L  I AVL+DAEE+   +  ++ W+ +L D +Y A+D LD+++ EAL   
Sbjct: 36  ENLLERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLN 95

Query: 232 V 233
           +
Sbjct: 96  I 96

BLAST of Spg014794 vs. TAIR 10
Match: AT3G14460.1 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 110.9 bits (276), Expect = 4.5e-24
Identity = 107/379 (28.23%), Postives = 175/379 (46.17%), Query Frame = 0

Query: 264 MHDVIRELARKVAGKKLYVRGNLNNDYV--VSEQTRHISFDYVIISWRDVLSKLRKAKGL 323
           MHD++ +LA+ V+G   +    L +D +  +   TRH SF             +  A+ L
Sbjct: 487 MHDLMNDLAKAVSGDFCF---RLEDDNIPEIPSTTRHFSFSRSQCDASVAFRSICGAEFL 546

Query: 324 RTFLLLHSYYANEN-KVNKTILDELFSSFSHLRVLSLYASNIYEVPNSIRKLRQLRYLDL 383
           RT L  +S  + E+ ++ + +L+ L ++ S LR+LSL    I  +P S++ L+ LRYLDL
Sbjct: 547 RTILPFNSPTSLESLQLTEKVLNPLLNALSGLRILSLSHYQITNLPKSLKGLKLLRYLDL 606

Query: 384 SENCRMELLPNSITRLQNLQTLKLTSCSGLKELPRDTSNLVNLRHLDFPSKKIRHMPKGM 443
           S + +++ LP  +  L NLQTL L++C  L  LP+  + L+NLR LD     +  MP G+
Sbjct: 607 S-STKIKELPEFVCTLCNLQTLLLSNCRDLTSLPKSIAELINLRLLDLVGTPLVEMPPGI 666

Query: 444 EKLTCLQRITLFELDCKRFSKPSELNNFNNLSTQSWRIIGLEQLRSSPPEVSSVNPKGGK 503
           +KL  LQ+++ F +     +   EL   ++L   + RI  L+ + +   E      K   
Sbjct: 667 KKLRSLQKLSNFVIGRLSGAGLHELKELSHL-RGTLRISELQNV-AFASEAKDAGLKRKP 726

Query: 504 DLRDLTLEWKLSQE-------ECEAASDERIMEGLEPHPNVEILRIDGCLRALKFLYLKD 563
            L  L L+W +             A   + ++  LEPHP+++   I+         +L D
Sbjct: 727 FLDGLILKWTVKGSGFVPGSFNALACDQKEVLRMLEPHPHLKTFCIESYQGGAFPKWLGD 786

Query: 564 LRCLKFIDKNDPYSSSVS----------FPSLEKLYLEDISNLEGWWESGESRTGETEKE 623
                F        SS +           PSL+ L +E  + L+      +   GE    
Sbjct: 787 ---SSFFGITSVTLSSCNLCISLPPVGQLPSLKYLSIEKFNILQK--VGLDFFFGENNSR 846

HSP 2 Score: 50.8 bits (120), Expect = 5.5e-06
Identity = 33/96 (34.38%), Postives = 50/96 (52.08%), Query Frame = 0

Query: 141 MAEGVLFTVAGEILMKLTSHASQRLGMLCGFEDD---LNKLKSNLSAIQAVLLDAEEQPT 200
           MA   L + A  ++ ++  + SQ L  LC  +     L +LK  L     VL DA+++  
Sbjct: 1   MANSYLSSCANVMVERI--NTSQELVELCKGKSSSALLKRLKVALVTANPVLADADQRAE 60

Query: 201 KSHSLKDWLEKLGDALYDADDVLDELSGEALHREVM 234
               +K WL  + DA + A+D+LDEL  EAL R V+
Sbjct: 61  HVREVKHWLTGIKDAFFQAEDILDELQTEALRRRVV 94

BLAST of Spg014794 vs. TAIR 10
Match: AT5G09680.1 (reduced lateral root formation )

HSP 1 Score: 75.9 bits (185), Expect = 1.6e-13
Identity = 34/38 (89.47%), Postives = 35/38 (92.11%), Query Frame = 0

Query: 712 TAARKPATRTKVPFEKGYSQMDWLKLTRTHPDLAGICG 750
           T ARKP TRTKVPFEKGYSQMDWLKLTRTHPDLAG+ G
Sbjct: 85  TPARKPITRTKVPFEKGYSQMDWLKLTRTHPDLAGLKG 122

BLAST of Spg014794 vs. TAIR 10
Match: AT5G09680.2 (reduced lateral root formation )

HSP 1 Score: 75.9 bits (185), Expect = 1.6e-13
Identity = 34/38 (89.47%), Postives = 35/38 (92.11%), Query Frame = 0

Query: 712 TAARKPATRTKVPFEKGYSQMDWLKLTRTHPDLAGICG 750
           T ARKP TRTKVPFEKGYSQMDWLKLTRTHPDLAG+ G
Sbjct: 85  TPARKPITRTKVPFEKGYSQMDWLKLTRTHPDLAGLKG 122

BLAST of Spg014794 vs. TAIR 10
Match: AT1G69550.1 (disease resistance protein (TIR-NBS-LRR class) )

HSP 1 Score: 71.6 bits (174), Expect = 3.0e-12
Identity = 88/313 (28.12%), Postives = 137/313 (43.77%), Query Frame = 0

Query: 342  LDELFSSFSHL-RVLSLY---ASNIYEVPNSIRKLRQLRYLDLSENCRMELLPNSITRLQ 401
            L EL SS  +L  +  LY    S++ E+P+SI  L  L+ LDLS    +  LP SI  L 
Sbjct: 945  LVELPSSIGNLINLQELYLSECSSLVELPSSIGNLINLKKLDLSGCSSLVELPLSIGNLI 1004

Query: 402  NLQTLKLTSCSGLKELPRDTSNLVNLRHLDFPS-KKIRHMPKGMEKLTCLQRITLFELDC 461
            NL+TL L+ CS L ELP    NL+NL+ L       +  +P  +  L  L+++ L    C
Sbjct: 1005 NLKTLNLSECSSLVELPSSIGNLINLQELYLSECSSLVELPSSIGNLINLKKLDL--SGC 1064

Query: 462  KRFSK-PSELNNFNNLSTQSWRIIGLEQLRSSPPEVSSVNPKGGKDLRDLTLEWKLSQEE 521
                + P  + N  NL T +  + G   L   P  + ++N      L+ L L    S  E
Sbjct: 1065 SSLVELPLSIGNLINLKTLN--LSGCSSLVELPSSIGNLN------LKKLDLSGCSSLVE 1124

Query: 522  CEAASDERIMEGLEPHPNVEILRIDGC------------LRALKFLYLKDLRCLKFIDKN 581
              ++    I        N++ L + GC            L  L+ LYL +  C   ++  
Sbjct: 1125 LPSSIGNLI--------NLKKLDLSGCSSLVELPLSIGNLINLQELYLSE--CSSLVELP 1184

Query: 582  DPYSSSVSFPSLEKLYLEDISNLEGWWESGESRTGETEKEGLPPTFPQLQFLYIYHCPKL 637
               SS  +  +L++LYL + S+L              E          L+ L +  C KL
Sbjct: 1185 ---SSIGNLINLQELYLSECSSL-------------VELPSSIGNLINLKKLDLNKCTKL 1221