Spg014781 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg014781
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionGlycerol kinase
Locationscaffold3: 47261736 .. 47264224 (+)
RNA-Seq ExpressionSpg014781
SyntenySpg014781
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCGTAATTTCATAGGTCGCTATTTAGTTGGGTTCATCATCGCGGTCTTAATTTCTCTCAGATTGAAGATTTTGGGTTGAGATCGGGAAGTGAAGAAGGAGAATCGAAGATAATGGCGAAGGCAGAGGATGCTTTTGTTGGTTCAATCGATCAAGGAACCACCAGTACCAGATTTATCATCTACGATCGTCGTTCTCGACCGATTGGATCTCACCAGGTCGAGTTCACTCAATTCTACCCTGAAGCCGGGTGAGTCGACCGATGCTTTTAATACTCATTCTATCTCTGCTCGATCTCTCTCTGTTATTACTTTTCAGAGTCTGATTTAATCTTTGTCTTTGTAGATGGGTGGAGCATAATCCGGTGGAAATTCTGGAGAGCGTGAAGGTTTGTATGGCCAAAGCTCTAGACAAGGCCACGGCGGCGGGTTATAATGTGGATAAGGGTTTGAAGGCGATTGGTTTAACCAATCAGAGAGAGACAACTCTGGTTTGGAGCAAATCTACTGGCTCTCCCCTCCATAACGCCATCGTTTGGATGGATGTTCGTACCAGTTCTATTTGCAGGTACCCTGTTTTTCCTTGTTTTACGTGTTATCCCCCATTTCCCCATTTTTCTGTGTTATCATTACACGGATTGATATTTAGATTCTGGGTTTTCGATTAATTAATACCATTTGCAGCAAGATCGTCTAATTTAGGATTTGGTTTCGTTGTTCTGATTCCAAACTTCTTATGTCTTCTTCTTTTAACTGTACTTAAGAAATGGGATTTTGTGTTCCTTGAGCTACATCTATGGATTTAGGCGGATGTATTTTGAATTCCTTTTATCTTGATATGGTAGAAAATTGGAGAAGGAGTTATCTGGTGGGAGAACTCATTTTGGGGATACCTGTGGTCTGCCCATAAGCACTTATTTCAGTGCAGTGAAGTTGCTTTGGCTGATGGAAAATGTGGAGGATGTTAGGGAAAGTGTAAAGAAGGGAGATGCTTTATTTGGGACTATAGACACTTGGTTGATATGGAACTTAACTGGCGGTGTCAATGGAGGATTGCATGTGACTGATGTTTCAAATGCCTCGCGAACGATGTTGATGAACCTCAAGACTCTTGACTGGGACAAACCGACATTGCAAACACTTGGAATACCAGCACAAATCTTGCCTAAGATTGTTAGCAACTCTGAGGTCATTGGGAAAATTGCCCAAGGTTGGCCAATTACTGGTGTTCCCATTTCAGGATGTTTAGGCGACCAACATGCTGCAATGCTTGGTCAAGCTTGTAGGAAAGGTGAGGCTAAAAGCACATATGGAACGGGTGCTTTTATACTTCTCAACACAGGGGAAGAAGTTGTTCACTCTAAGCACGGCCTTCTGACCACTTTGGCATTCAAGCTTGGCCAGGATGCTCCAACAAATTATGCTCTTGAAGGCTCTATTGCCATTGCTGGAGCTGCAGTTCAGTGGCTTAGAGACGGTCTTGGAATTATCAGCAGTGCACAAGAGATCGAGGAATTGGCATCACAGGTTAAGTCAACTGGTGGGGTGTATTTTGTACCTGCCTTTAATGGGTTGTTTGCTCCGTGGTGGCGCGACGATGCTCGTGGAGTCTGTATTGGGATCACAAGGTTTACAAGCAAGGCTCATATTGCTCGAGCCGTGCTTGAGAGTATGTGTTTTCAAGTGAAAGACGTGTTGGATTCGATGCACAAAGATGGTGGAGAGAAGGGTGAGGTCAAGAATGAGAAAGGGGAATTCTTGCTTAGAGTGGATGGTGGTGCAACTGTCAACAATCTTTTGATGCAAATTCAGGTTTGAGATTTCTATTTCATATGGTACATGTTTTGTGACTCCAACCATTTGAATGAGTAGTTTTTCTTTATCATTATTGTTGACGTTATTGGTTTGTGATCAATCTTTGCAGGCGGACTTGTTGGGAAGTCCAGTGGTGAGACCGGCGGACATCGAGACAACAGCCCTTGGAGCAGCTTATGCGGCAGGATTAGCCGTCGGAGTTTGGACAAAAGATGAGATTTTTGACTCAGGAGAAAGGGTTAAGAGTGCAACCACTTTCCGTCCTGTATTAGAAGATGAGTCGAGGAAGAAGAAGGTGGAATCATGGTGCAAAGCCGTTTCAAGAACTTTTGATTTGGCTGATCTTTCCCTTTAATTTCATGCCTCATCTTCAACTTTACAACATAACATAACATAAAATATTTCCTTCTTTGCTTGTATAATTGTTTTCTTTTTAAATGTTTCCATGAATTATATTCGCCCCTAATAAATTTAAACAGAGTGAAGGAATAAATTTCATCACTTCTGGATTCAAGTTCTTATGTGTGATTATTGTGCATTTAAATTGGATTGAAAGCTAGTTTTAAAGTGATCATGTATTTTTAACTCAAAAGCAATTTAATTGATCCATAGAATTTTTACCTATTGATAACTCATTTATGTACTTTTTTTTTTTATTTTTTTTATTTTTTTTTAAT

mRNA sequence

ATGGCGAAGGCAGAGGATGCTTTTGTTGGTTCAATCGATCAAGGAACCACCAGTACCAGATTTATCATCTACGATCGTCGTTCTCGACCGATTGGATCTCACCAGGTCGAGTTCACTCAATTCTACCCTGAAGCCGGATGGGTGGAGCATAATCCGGTGGAAATTCTGGAGAGCGTGAAGGTTTGTATGGCCAAAGCTCTAGACAAGGCCACGGCGGCGGGTTATAATGTGGATAAGGGTTTGAAGGCGATTGGTTTAACCAATCAGAGAGAGACAACTCTGGTTTGGAGCAAATCTACTGGCTCTCCCCTCCATAACGCCATCGTTTGGATGGATGTTCGTACCAGTTCTATTTGCAGAAAATTGGAGAAGGAGTTATCTGGTGGGAGAACTCATTTTGGGGATACCTGTGGTCTGCCCATAAGCACTTATTTCAGTGCAGTGAAGTTGCTTTGGCTGATGGAAAATGTGGAGGATGTTAGGGAAAGTGTAAAGAAGGGAGATGCTTTATTTGGGACTATAGACACTTGGTTGATATGGAACTTAACTGGCGGTGTCAATGGAGGATTGCATGTGACTGATGTTTCAAATGCCTCGCGAACGATGTTGATGAACCTCAAGACTCTTGACTGGGACAAACCGACATTGCAAACACTTGGAATACCAGCACAAATCTTGCCTAAGATTGTTAGCAACTCTGAGGTCATTGGGAAAATTGCCCAAGGTTGGCCAATTACTGGTGTTCCCATTTCAGGATGTTTAGGCGACCAACATGCTGCAATGCTTGGTCAAGCTTGTAGGAAAGGTGAGGCTAAAAGCACATATGGAACGGGTGCTTTTATACTTCTCAACACAGGGGAAGAAGTTGTTCACTCTAAGCACGGCCTTCTGACCACTTTGGCATTCAAGCTTGGCCAGGATGCTCCAACAAATTATGCTCTTGAAGGCTCTATTGCCATTGCTGGAGCTGCAGTTCAGTGGCTTAGAGACGGTCTTGGAATTATCAGCAGTGCACAAGAGATCGAGGAATTGGCATCACAGGTTAAGTCAACTGGTGGGGTGTATTTTGTACCTGCCTTTAATGGGTTGTTTGCTCCGTGGTGGCGCGACGATGCTCGTGGAGTCTGTATTGGGATCACAAGGTTTACAAGCAAGGCTCATATTGCTCGAGCCGTGCTTGAGAGTATGTGTTTTCAAGTGAAAGACGTGTTGGATTCGATGCACAAAGATGGTGGAGAGAAGGGTGAGGTCAAGAATGAGAAAGGGGAATTCTTGCTTAGAGTGGATGGTGGTGCAACTGTCAACAATCTTTTGATGCAAATTCAGGCGGACTTGTTGGGAAGTCCAGTGGTGAGACCGGCGGACATCGAGACAACAGCCCTTGGAGCAGCTTATGCGGCAGGATTAGCCGTCGGAGTTTGGACAAAAGATGAGATTTTTGACTCAGGAGAAAGGGTTAAGAGTGCAACCACTTTCCGTCCTGTATTAGAAGATGAGTCGAGGAAGAAGAAGGTGGAATCATGGTGCAAAGCCGTTTCAAGAACTTTTGATTTGGCTGATCTTTCCCTTTAA

Coding sequence (CDS)

ATGGCGAAGGCAGAGGATGCTTTTGTTGGTTCAATCGATCAAGGAACCACCAGTACCAGATTTATCATCTACGATCGTCGTTCTCGACCGATTGGATCTCACCAGGTCGAGTTCACTCAATTCTACCCTGAAGCCGGATGGGTGGAGCATAATCCGGTGGAAATTCTGGAGAGCGTGAAGGTTTGTATGGCCAAAGCTCTAGACAAGGCCACGGCGGCGGGTTATAATGTGGATAAGGGTTTGAAGGCGATTGGTTTAACCAATCAGAGAGAGACAACTCTGGTTTGGAGCAAATCTACTGGCTCTCCCCTCCATAACGCCATCGTTTGGATGGATGTTCGTACCAGTTCTATTTGCAGAAAATTGGAGAAGGAGTTATCTGGTGGGAGAACTCATTTTGGGGATACCTGTGGTCTGCCCATAAGCACTTATTTCAGTGCAGTGAAGTTGCTTTGGCTGATGGAAAATGTGGAGGATGTTAGGGAAAGTGTAAAGAAGGGAGATGCTTTATTTGGGACTATAGACACTTGGTTGATATGGAACTTAACTGGCGGTGTCAATGGAGGATTGCATGTGACTGATGTTTCAAATGCCTCGCGAACGATGTTGATGAACCTCAAGACTCTTGACTGGGACAAACCGACATTGCAAACACTTGGAATACCAGCACAAATCTTGCCTAAGATTGTTAGCAACTCTGAGGTCATTGGGAAAATTGCCCAAGGTTGGCCAATTACTGGTGTTCCCATTTCAGGATGTTTAGGCGACCAACATGCTGCAATGCTTGGTCAAGCTTGTAGGAAAGGTGAGGCTAAAAGCACATATGGAACGGGTGCTTTTATACTTCTCAACACAGGGGAAGAAGTTGTTCACTCTAAGCACGGCCTTCTGACCACTTTGGCATTCAAGCTTGGCCAGGATGCTCCAACAAATTATGCTCTTGAAGGCTCTATTGCCATTGCTGGAGCTGCAGTTCAGTGGCTTAGAGACGGTCTTGGAATTATCAGCAGTGCACAAGAGATCGAGGAATTGGCATCACAGGTTAAGTCAACTGGTGGGGTGTATTTTGTACCTGCCTTTAATGGGTTGTTTGCTCCGTGGTGGCGCGACGATGCTCGTGGAGTCTGTATTGGGATCACAAGGTTTACAAGCAAGGCTCATATTGCTCGAGCCGTGCTTGAGAGTATGTGTTTTCAAGTGAAAGACGTGTTGGATTCGATGCACAAAGATGGTGGAGAGAAGGGTGAGGTCAAGAATGAGAAAGGGGAATTCTTGCTTAGAGTGGATGGTGGTGCAACTGTCAACAATCTTTTGATGCAAATTCAGGCGGACTTGTTGGGAAGTCCAGTGGTGAGACCGGCGGACATCGAGACAACAGCCCTTGGAGCAGCTTATGCGGCAGGATTAGCCGTCGGAGTTTGGACAAAAGATGAGATTTTTGACTCAGGAGAAAGGGTTAAGAGTGCAACCACTTTCCGTCCTGTATTAGAAGATGAGTCGAGGAAGAAGAAGGTGGAATCATGGTGCAAAGCCGTTTCAAGAACTTTTGATTTGGCTGATCTTTCCCTTTAA

Protein sequence

MAKAEDAFVGSIDQGTTSTRFIIYDRRSRPIGSHQVEFTQFYPEAGWVEHNPVEILESVKVCMAKALDKATAAGYNVDKGLKAIGLTNQRETTLVWSKSTGSPLHNAIVWMDVRTSSICRKLEKELSGGRTHFGDTCGLPISTYFSAVKLLWLMENVEDVRESVKKGDALFGTIDTWLIWNLTGGVNGGLHVTDVSNASRTMLMNLKTLDWDKPTLQTLGIPAQILPKIVSNSEVIGKIAQGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVHSKHGLLTTLAFKLGQDAPTNYALEGSIAIAGAAVQWLRDGLGIISSAQEIEELASQVKSTGGVYFVPAFNGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDGGEKGEVKNEKGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTKDEIFDSGERVKSATTFRPVLEDESRKKKVESWCKAVSRTFDLADLSL
Homology
BLAST of Spg014781 vs. NCBI nr
Match: XP_008461141.1 (PREDICTED: glycerol kinase [Cucumis melo])

HSP 1 Score: 1012.7 bits (2617), Expect = 1.2e-291
Identity = 498/523 (95.22%), Postives = 510/523 (97.51%), Query Frame = 0

Query: 1   MAKAEDAFVGSIDQGTTSTRFIIYDRRSRPIGSHQVEFTQFYPEAGWVEHNPVEILESVK 60
           MAKAED FVGSIDQGTTSTRFIIYDR SRPIGSHQ EFTQFYPEAGWVEH+PVEILESVK
Sbjct: 1   MAKAEDVFVGSIDQGTTSTRFIIYDRHSRPIGSHQAEFTQFYPEAGWVEHDPVEILESVK 60

Query: 61  VCMAKALDKATAAGYNVDKGLKAIGLTNQRETTLVWSKSTGSPLHNAIVWMDVRTSSICR 120
           VCMAKALDKATAAGYNVD GLKAIGLTNQRETTLVWSKS+GSPLHNAIVWMD RTSSICR
Sbjct: 61  VCMAKALDKATAAGYNVDNGLKAIGLTNQRETTLVWSKSSGSPLHNAIVWMDARTSSICR 120

Query: 121 KLEKELSGGRTHFGDTCGLPISTYFSAVKLLWLMENVEDVRESVKKGDALFGTIDTWLIW 180
           KLEKELSGGRTHF DTCGLPISTYFSAVKL+WLMENVE VRES++KGDALFGTIDTWLIW
Sbjct: 121 KLEKELSGGRTHFVDTCGLPISTYFSAVKLIWLMENVEAVRESIEKGDALFGTIDTWLIW 180

Query: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLDWDKPTLQTLGIPAQILPKIVSNSEVIGKIA 240
           NLTGGVNGGLHVTDVSNASRTMLMNLKTLDWDKPTL+TLGIPAQILPKIVSNSEVIGKI+
Sbjct: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLDWDKPTLETLGIPAQILPKIVSNSEVIGKIS 240

Query: 241 QGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVHSKHGLLTTL 300
           QGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVV SKHGLLTTL
Sbjct: 241 QGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTL 300

Query: 301 AFKLGQDAPTNYALEGSIAIAGAAVQWLRDGLGIISSAQEIEELASQVKSTGGVYFVPAF 360
           AFKLG+DAPTNYALEGSIAIAGAAVQWLRD LGIISSAQEIEELA QV+STGGVYFVPAF
Sbjct: 301 AFKLGRDAPTNYALEGSIAIAGAAVQWLRDSLGIISSAQEIEELALQVQSTGGVYFVPAF 360

Query: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDGGEKGEVKNE 420
           NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKD GEKGEVKNE
Sbjct: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAGEKGEVKNE 420

Query: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTKDEIF 480
           KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVG+W+KDEIF
Sbjct: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGIWSKDEIF 480

Query: 481 DSGERVKSATTFRPVLEDESRKKKVESWCKAVSRTFDLADLSL 524
           DSGERVKSATTF PVLE+E RKKKVESWCKAVSRTFDLADLSL
Sbjct: 481 DSGERVKSATTFYPVLEEELRKKKVESWCKAVSRTFDLADLSL 523

BLAST of Spg014781 vs. NCBI nr
Match: KAA0058737.1 (glycerol kinase [Cucumis melo var. makuwa])

HSP 1 Score: 1010.7 bits (2612), Expect = 4.4e-291
Identity = 497/523 (95.03%), Postives = 509/523 (97.32%), Query Frame = 0

Query: 1   MAKAEDAFVGSIDQGTTSTRFIIYDRRSRPIGSHQVEFTQFYPEAGWVEHNPVEILESVK 60
           MAKAED FVGSIDQGTTSTRFIIYDR SRPIGSHQ EFTQFYPEAGWVEH+PVEILESVK
Sbjct: 1   MAKAEDVFVGSIDQGTTSTRFIIYDRHSRPIGSHQAEFTQFYPEAGWVEHDPVEILESVK 60

Query: 61  VCMAKALDKATAAGYNVDKGLKAIGLTNQRETTLVWSKSTGSPLHNAIVWMDVRTSSICR 120
           VCMAKALDKATAAGYNVD GLKAIGLTNQRETTLVWSKS+GSPLHNAIVWMD RTSSICR
Sbjct: 61  VCMAKALDKATAAGYNVDNGLKAIGLTNQRETTLVWSKSSGSPLHNAIVWMDARTSSICR 120

Query: 121 KLEKELSGGRTHFGDTCGLPISTYFSAVKLLWLMENVEDVRESVKKGDALFGTIDTWLIW 180
           KLEKELSGGRTHF DTCGLPISTYFSAVKL+WLMENVE VRES++KGDALFGTIDTWLIW
Sbjct: 121 KLEKELSGGRTHFVDTCGLPISTYFSAVKLIWLMENVEAVRESIEKGDALFGTIDTWLIW 180

Query: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLDWDKPTLQTLGIPAQILPKIVSNSEVIGKIA 240
           NLTGGVNGGLHVTDVSNASRTMLMNLKTLDWDKPTL+TLGIPAQILPKIVSNSEVIGKI+
Sbjct: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLDWDKPTLETLGIPAQILPKIVSNSEVIGKIS 240

Query: 241 QGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVHSKHGLLTTL 300
           QGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVV SKHGLLTTL
Sbjct: 241 QGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTL 300

Query: 301 AFKLGQDAPTNYALEGSIAIAGAAVQWLRDGLGIISSAQEIEELASQVKSTGGVYFVPAF 360
           AFKLG+DAPTNYALEGSIAIAGAAVQWLRD LGIISSAQEIEELA QV+STGGVYFVPAF
Sbjct: 301 AFKLGRDAPTNYALEGSIAIAGAAVQWLRDSLGIISSAQEIEELALQVQSTGGVYFVPAF 360

Query: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDGGEKGEVKNE 420
           NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKD GEKGEVKNE
Sbjct: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAGEKGEVKNE 420

Query: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTKDEIF 480
           KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVG+W+KDEIF
Sbjct: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGIWSKDEIF 480

Query: 481 DSGERVKSATTFRPVLEDESRKKKVESWCKAVSRTFDLADLSL 524
           DSGERVK ATTF PVLE+E RKKKVESWCKAVSRTFDLADLSL
Sbjct: 481 DSGERVKRATTFYPVLEEELRKKKVESWCKAVSRTFDLADLSL 523

BLAST of Spg014781 vs. NCBI nr
Match: XP_038899608.1 (glycerol kinase [Benincasa hispida] >XP_038899609.1 glycerol kinase [Benincasa hispida])

HSP 1 Score: 1006.9 bits (2602), Expect = 6.4e-290
Identity = 495/523 (94.65%), Postives = 509/523 (97.32%), Query Frame = 0

Query: 1   MAKAEDAFVGSIDQGTTSTRFIIYDRRSRPIGSHQVEFTQFYPEAGWVEHNPVEILESVK 60
           MAKAE+ FVGSIDQGTTSTRFIIYDRRSR IGSHQ EFTQFYPEAGWVEH+P+EILESVK
Sbjct: 1   MAKAEEVFVGSIDQGTTSTRFIIYDRRSRAIGSHQAEFTQFYPEAGWVEHDPMEILESVK 60

Query: 61  VCMAKALDKATAAGYNVDKGLKAIGLTNQRETTLVWSKSTGSPLHNAIVWMDVRTSSICR 120
           VCMAKALDKATAAGYNVD GLKAIGLTNQRETTLVWSKSTGSPLHNAIVWMD RTSSICR
Sbjct: 61  VCMAKALDKATAAGYNVDNGLKAIGLTNQRETTLVWSKSTGSPLHNAIVWMDARTSSICR 120

Query: 121 KLEKELSGGRTHFGDTCGLPISTYFSAVKLLWLMENVEDVRESVKKGDALFGTIDTWLIW 180
           KLEKELSGGRTHF DTCGLPISTYFSAVKL+WLMENVEDVRES+KKGDALFGT+DTWLIW
Sbjct: 121 KLEKELSGGRTHFVDTCGLPISTYFSAVKLIWLMENVEDVRESIKKGDALFGTVDTWLIW 180

Query: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLDWDKPTLQTLGIPAQILPKIVSNSEVIGKIA 240
           NLTGGVNGGLHVTDVSNASRTMLMNLKTLDWDKPTL+TLGIPA+ILPKIVSNSEVIGKI+
Sbjct: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLDWDKPTLETLGIPAKILPKIVSNSEVIGKIS 240

Query: 241 QGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVHSKHGLLTTL 300
            GWPITGVPISGCLGDQHAAMLGQACR+GEAKSTYGTGAFILLNTGEEVV SKHGLLTTL
Sbjct: 241 LGWPITGVPISGCLGDQHAAMLGQACRRGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTL 300

Query: 301 AFKLGQDAPTNYALEGSIAIAGAAVQWLRDGLGIISSAQEIEELASQVKSTGGVYFVPAF 360
           AFKLG  APTNYALEGSIAIAGAAVQWLRD LGIISSA+EIEELASQV+STGGVYFVPAF
Sbjct: 301 AFKLGPVAPTNYALEGSIAIAGAAVQWLRDSLGIISSAKEIEELASQVQSTGGVYFVPAF 360

Query: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDGGEKGEVKNE 420
           NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKD GEKGEVKNE
Sbjct: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAGEKGEVKNE 420

Query: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTKDEIF 480
           KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVW+KDEIF
Sbjct: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWSKDEIF 480

Query: 481 DSGERVKSATTFRPVLEDESRKKKVESWCKAVSRTFDLADLSL 524
           DSGERVKSATTF PVLE+E RKKKVESWCKAVSRTFDLADLSL
Sbjct: 481 DSGERVKSATTFHPVLEEELRKKKVESWCKAVSRTFDLADLSL 523

BLAST of Spg014781 vs. NCBI nr
Match: XP_004135863.1 (glycerol kinase [Cucumis sativus] >KGN45247.1 hypothetical protein Csa_015818 [Cucumis sativus])

HSP 1 Score: 1003.4 bits (2593), Expect = 7.0e-289
Identity = 495/523 (94.65%), Postives = 507/523 (96.94%), Query Frame = 0

Query: 1   MAKAEDAFVGSIDQGTTSTRFIIYDRRSRPIGSHQVEFTQFYPEAGWVEHNPVEILESVK 60
           MAKAED FVGSIDQGTTS+RFIIYDR SRPIGSHQ EFTQFYPEAGWVEH+PVEILESVK
Sbjct: 1   MAKAEDVFVGSIDQGTTSSRFIIYDRHSRPIGSHQAEFTQFYPEAGWVEHDPVEILESVK 60

Query: 61  VCMAKALDKATAAGYNVDKGLKAIGLTNQRETTLVWSKSTGSPLHNAIVWMDVRTSSICR 120
           VCMAKALDKATAAGYNVD GLKAIGLTNQRETTLVWSKSTG PLH+AIVWMD RTSSICR
Sbjct: 61  VCMAKALDKATAAGYNVDNGLKAIGLTNQRETTLVWSKSTGFPLHHAIVWMDARTSSICR 120

Query: 121 KLEKELSGGRTHFGDTCGLPISTYFSAVKLLWLMENVEDVRESVKKGDALFGTIDTWLIW 180
           KLEKELSGGRTHF DTCGLPISTYFSAVKL+WLMENVE VRES+KK DALFGTIDTWLIW
Sbjct: 121 KLEKELSGGRTHFVDTCGLPISTYFSAVKLIWLMENVEAVRESIKKEDALFGTIDTWLIW 180

Query: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLDWDKPTLQTLGIPAQILPKIVSNSEVIGKIA 240
           NLTGGVNGGLHVTDVSNASRTMLMNLKTLDWDKPTL+TLGIPAQILPKIVSNSEVIGKI+
Sbjct: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLDWDKPTLETLGIPAQILPKIVSNSEVIGKIS 240

Query: 241 QGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVHSKHGLLTTL 300
           QGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVV SKHGLLTTL
Sbjct: 241 QGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTL 300

Query: 301 AFKLGQDAPTNYALEGSIAIAGAAVQWLRDGLGIISSAQEIEELASQVKSTGGVYFVPAF 360
           AFKLG DAPTNYALEGSIAIAGAAVQWLRD LGIISSAQEIE+LASQV+STGGVYFVPAF
Sbjct: 301 AFKLGPDAPTNYALEGSIAIAGAAVQWLRDSLGIISSAQEIEKLASQVQSTGGVYFVPAF 360

Query: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDGGEKGEVKNE 420
           NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKD  EKGEVKNE
Sbjct: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAVEKGEVKNE 420

Query: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTKDEIF 480
           KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVG+W+KDEIF
Sbjct: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGIWSKDEIF 480

Query: 481 DSGERVKSATTFRPVLEDESRKKKVESWCKAVSRTFDLADLSL 524
           DSGERVKSATTF PVLE+E RKKKVESWCKAVSRTFDLADLSL
Sbjct: 481 DSGERVKSATTFYPVLEEELRKKKVESWCKAVSRTFDLADLSL 523

BLAST of Spg014781 vs. NCBI nr
Match: KAG6575609.1 (Glycerol kinase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 995.0 bits (2571), Expect = 2.5e-286
Identity = 489/523 (93.50%), Postives = 505/523 (96.56%), Query Frame = 0

Query: 1   MAKAEDAFVGSIDQGTTSTRFIIYDRRSRPIGSHQVEFTQFYPEAGWVEHNPVEILESVK 60
           MAKAED FVGSIDQGTTSTRFIIYDR SRPIGSHQVEFTQFYPEAGWVEH+P+EILESV+
Sbjct: 1   MAKAEDVFVGSIDQGTTSTRFIIYDRCSRPIGSHQVEFTQFYPEAGWVEHDPMEILESVR 60

Query: 61  VCMAKALDKATAAGYNVDKGLKAIGLTNQRETTLVWSKSTGSPLHNAIVWMDVRTSSICR 120
           VCMAKA+DKATAAGYNVD GLKAIGLTNQRETTLVWSKSTGSPLHNAIVWMD RTS+ICR
Sbjct: 61  VCMAKAVDKATAAGYNVDNGLKAIGLTNQRETTLVWSKSTGSPLHNAIVWMDARTSAICR 120

Query: 121 KLEKELSGGRTHFGDTCGLPISTYFSAVKLLWLMENVEDVRESVKKGDALFGTIDTWLIW 180
           KLE ELSGGRTHF  TCGLPISTYFSAVKLLWLMENV+DV ES+KKGDALFGTIDTWLIW
Sbjct: 121 KLENELSGGRTHFVGTCGLPISTYFSAVKLLWLMENVKDVNESIKKGDALFGTIDTWLIW 180

Query: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLDWDKPTLQTLGIPAQILPKIVSNSEVIGKIA 240
           NLTGGVNGGLHVTDVSNASRTMLMNLKTL+WDKPTL+TLGIPAQILPKIVSNSEVIGKI 
Sbjct: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLEWDKPTLETLGIPAQILPKIVSNSEVIGKIV 240

Query: 241 QGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVHSKHGLLTTL 300
           QGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVV SKHGLLTTL
Sbjct: 241 QGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTL 300

Query: 301 AFKLGQDAPTNYALEGSIAIAGAAVQWLRDGLGIISSAQEIEELASQVKSTGGVYFVPAF 360
           AFKLG+DA  NYALEGSIAIAGAAVQWLRD LGIISSA+EIEELASQVKSTGG+YFVPAF
Sbjct: 301 AFKLGKDARANYALEGSIAIAGAAVQWLRDSLGIISSAKEIEELASQVKSTGGIYFVPAF 360

Query: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDGGEKGEVKNE 420
           NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKD  EKGEVKNE
Sbjct: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAREKGEVKNE 420

Query: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTKDEIF 480
           KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWT+DEIF
Sbjct: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTEDEIF 480

Query: 481 DSGERVKSATTFRPVLEDESRKKKVESWCKAVSRTFDLADLSL 524
            +GERV+SATTF PVLE+E RKKKVESWCKAVSRTFDLADLSL
Sbjct: 481 SAGERVESATTFHPVLEEELRKKKVESWCKAVSRTFDLADLSL 523

BLAST of Spg014781 vs. ExPASy Swiss-Prot
Match: Q9M8L4 (Glycerol kinase OS=Arabidopsis thaliana OX=3702 GN=GLPK PE=1 SV=1)

HSP 1 Score: 891.3 bits (2302), Expect = 5.1e-258
Identity = 428/523 (81.84%), Postives = 478/523 (91.40%), Query Frame = 0

Query: 1   MAKAEDAFVGSIDQGTTSTRFIIYDRRSRPIGSHQVEFTQFYPEAGWVEHNPVEILESVK 60
           MAK E+ F+GSIDQGTTSTRFIIYD  +RP+ SHQVEFTQFYPEAGWVEH+P+EILESVK
Sbjct: 1   MAK-ENGFIGSIDQGTTSTRFIIYDHDARPVASHQVEFTQFYPEAGWVEHDPMEILESVK 60

Query: 61  VCMAKALDKATAAGYNVDKGLKAIGLTNQRETTLVWSKSTGSPLHNAIVWMDVRTSSICR 120
           VC+AKALDKATA G+NVD GLKAIGLT+QRETT+VWSKSTG PLH AIVWMD RTSSICR
Sbjct: 61  VCIAKALDKATADGHNVDGGLKAIGLTDQRETTVVWSKSTGLPLHKAIVWMDARTSSICR 120

Query: 121 KLEKELSGGRTHFGDTCGLPISTYFSAVKLLWLMENVEDVRESVKKGDALFGTIDTWLIW 180
           +LEKELSGGR+HF ++CGLPISTYFSA+KLLWLMENV+DV++++KKGDA+FGTIDTWLIW
Sbjct: 121 RLEKELSGGRSHFVESCGLPISTYFSAMKLLWLMENVDDVKDAIKKGDAIFGTIDTWLIW 180

Query: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLDWDKPTLQTLGIPAQILPKIVSNSEVIGKIA 240
           N+TGG+NGGLHVTDV+NASRTMLMNLKTL WD+ TL+TLGIPA+ILPKIVSNSEVIG+I 
Sbjct: 181 NMTGGINGGLHVTDVTNASRTMLMNLKTLSWDQDTLKTLGIPAEILPKIVSNSEVIGEIC 240

Query: 241 QGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVHSKHGLLTTL 300
           +GWPI G+ I+GCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGE  + S HGLLTTL
Sbjct: 241 KGWPIPGIKIAGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEVPIKSGHGLLTTL 300

Query: 301 AFKLGQDAPTNYALEGSIAIAGAAVQWLRDGLGIISSAQEIEELASQVKSTGGVYFVPAF 360
           A+KLG  A TNYALEGSIAIAGAAVQWLRD LGII SA EIE+LA+ V STGGVYFVPAF
Sbjct: 301 AYKLGPQAQTNYALEGSIAIAGAAVQWLRDSLGIIKSASEIEDLAAMVDSTGGVYFVPAF 360

Query: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDGGEKGEVKNE 420
           NGLFAPWWR+DARGVCIGITRFT+K+HIARAVLESMCFQVKDVLDSM+KD GEKG + N 
Sbjct: 361 NGLFAPWWREDARGVCIGITRFTNKSHIARAVLESMCFQVKDVLDSMNKDAGEKGSLNNG 420

Query: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTKDEIF 480
           KGEFLLRVDGGAT NNLLMQIQADL+GSPVVRP DIETTALGAAYAAGLAVG W + +IF
Sbjct: 421 KGEFLLRVDGGATANNLLMQIQADLMGSPVVRPVDIETTALGAAYAAGLAVGFWKEADIF 480

Query: 481 DSGERVKSATTFRPVLEDESRKKKVESWCKAVSRTFDLADLSL 524
           +SGE+ K++  FRP +E+  RKKKV SWCKAV RTFDLADLS+
Sbjct: 481 ESGEKAKNSKVFRPAMEEGIRKKKVASWCKAVERTFDLADLSI 522

BLAST of Spg014781 vs. ExPASy Swiss-Prot
Match: Q54VT8 (Probable glycerol kinase OS=Dictyostelium discoideum OX=44689 GN=gk PE=3 SV=1)

HSP 1 Score: 544.7 bits (1402), Expect = 1.2e-153
Identity = 271/550 (49.27%), Postives = 363/550 (66.00%), Query Frame = 0

Query: 8   FVGSIDQGTTSTRFIIYDRRSRPIGSHQVEFTQFYPEAGWVEHNPVEILESVKVCMAKAL 67
           ++G+IDQGT+STRFI++D+    + SHQ+  TQ +P  GWVEH+  EILESV  C+   +
Sbjct: 4   YIGAIDQGTSSTRFILFDKNGDIVLSHQILLTQHHPHPGWVEHDGNEILESVNKCIQVVM 63

Query: 68  DKATAAGYNVDKGLKAIGLTNQRETTLVWSKSTGSPLHNAIVWMDVRTSSIC----RKLE 127
            +     +   + +KAIG+TNQRETT+VW K T  PL+NAIVW D RT  +      K +
Sbjct: 64  KQYYENNFGTKEDIKAIGITNQRETTIVWDKKTSKPLNNAIVWCDTRTKDLVNYFNNKAK 123

Query: 128 KELSG------------------------GRTHFGDTCGLPISTYFSAVKLLWLMENVED 187
           K +                           + +  + CGLP+S+YFS +KL WL +N E 
Sbjct: 124 KLIDDNNIIDNNSKSTTVVDGAQGECKLESKNYLREKCGLPLSSYFSGLKLKWLFDNCES 183

Query: 188 VRESVKKGDALFGTIDTWLIWNLTGGVNGGLHVTDVSNASRTMLMNLKTLDWDKPTLQTL 247
           VRE+  +GD L GTID+WL+WNLTG   G  H+TDV+NASRTMLMNLKTL WDK     L
Sbjct: 184 VREAYGRGDCLMGTIDSWLVWNLTG---GKCHITDVTNASRTMLMNLKTLSWDKELCDFL 243

Query: 248 GIPAQILPKIVSNSEVIGKIAQG--------WPITGVPISGCLGDQHAAMLGQAC-RKGE 307
            +P +ILP I S+SE+ G +  G         P+ G+PI+G LGDQ AAM+GQ C  KG+
Sbjct: 244 EVPIEILPNIHSSSEIYGHVTMGDDEQQQQQHPLHGIPIAGVLGDQQAAMVGQMCFEKGQ 303

Query: 308 AKSTYGTGAFILLNTGEEVVHSKHGLLTTLAFKLGQDAPTNYALEGSIAIAGAAVQWLRD 367
           AK+TYGTG F+L NTG ++VHS++GLLTT+ ++ G+D+P  YALEG +A+AG+ V+WL D
Sbjct: 304 AKNTYGTGCFLLYNTGNDIVHSRNGLLTTVCYQFGKDSPPIYALEGGVAVAGSGVRWLID 363

Query: 368 GLGIISSAQEIEELASQVKSTGGVYFVPAFNGLFAPWWRDDARGVCIGITRFTSKAHIAR 427
            +GI  S+QEIE+LA  V+ TGG+YFVPAF+GLFAP+WRDDARGV +G+T  T++ HIAR
Sbjct: 364 NMGIAESSQEIEDLAKSVQDTGGMYFVPAFSGLFAPYWRDDARGVMVGLTHHTNRCHIAR 423

Query: 428 AVLESMCFQVKDVLDSMHKDGGEKGEVKNEKGEFLLRVDGGATVNNLLMQIQADLLGSPV 487
           +VLES C Q  +VLD+M KD G K           LRVDGG   NNLL+QIQ+DLLG PV
Sbjct: 424 SVLESTCLQTFEVLDAMQKDSGNK--------LVELRVDGGMAKNNLLLQIQSDLLGLPV 483

Query: 488 VRPADIETTALGAAYAAGLAVGVWTKDEIFDSGERVKSATTFRPVLEDESRKKKVESWCK 521
           V+P  +ETT  GAA+AAG+A GVW +   F  G +      F P L++  + +K++ W K
Sbjct: 484 VKPISLETTCFGAAFAAGIATGVWKETMQFKIGGK------FTPQLDENHKTQKLKEWKK 536

BLAST of Spg014781 vs. ExPASy Swiss-Prot
Match: Q828K5 (Glycerol kinase 1 OS=Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680) OX=227882 GN=glpK1 PE=3 SV=1)

HSP 1 Score: 540.0 bits (1390), Expect = 2.9e-152
Identity = 270/512 (52.73%), Postives = 362/512 (70.70%), Query Frame = 0

Query: 8   FVGSIDQGTTSTRFIIYDRRSRPIGSHQVEFTQFYPEAGWVEHNPVEILESVKVCMAKAL 67
           F+ +IDQGTTS+R I++DR  R +   Q E  Q +P+ GWVEHN  EI  +V+  +A A+
Sbjct: 10  FIAAIDQGTTSSRCIVFDRDGRIVSVDQKEHEQIFPKPGWVEHNAAEIWTNVQEVVAGAV 69

Query: 68  DKATAAGYNVDKGLKAIGLTNQRETTLVWSKSTGSPLHNAIVWMDVRTSSICRKLEKELS 127
           +K   AG   D  +KAIG+TNQRETTL+W K+TG P+HNA+VW D RT ++C++L + + 
Sbjct: 70  EK---AGITRD-DIKAIGITNQRETTLLWDKNTGEPVHNALVWQDTRTDALCKELGRNV- 129

Query: 128 GGRTHFGDTCGLPISTYFSAVKLLWLMENVEDVRESVKKGDALFGTIDTWLIWNLTGGVN 187
            G+  F    GLP+++YF+  K  WL++NVE +RE  + GD LFGT+DTW+IWNLTGGVN
Sbjct: 130 -GQDRFRRETGLPLASYFAGPKARWLLDNVEGLRERAEAGDILFGTMDTWVIWNLTGGVN 189

Query: 188 GGLHVTDVSNASRTMLMNLKTLDWDKPTLQTLGIPAQILPKIVSNSEVIGKIAQGW---P 247
           GG HVTDV+NASRTMLMNL T+ WD    +++G+P  +LP+I S++EV G+I  G     
Sbjct: 190 GGKHVTDVTNASRTMLMNLHTMQWDDKIAESIGVPLAMLPEIRSSAEVYGEITGGKLGDL 249

Query: 248 ITGVPISGCLGDQHAAMLGQAC-RKGEAKSTYGTGAFILLNTGEEVVHSKHGLLTTLAFK 307
           + G+P++  LGDQ AA+ GQ C  +GEAKSTYGTG F+L+NTG+++++S  GLLTT+ ++
Sbjct: 250 LGGIPVASALGDQQAALFGQTCFSEGEAKSTYGTGTFMLMNTGDKIINSYSGLLTTVGYQ 309

Query: 308 LGQDAPTNYALEGSIAIAGAAVQWLRDGLGIISSAQEIEELASQVKSTGGVYFVPAFNGL 367
           +G   P  YALEGSIA+ G+ VQW+RD +G+I SA EIE LAS V+  GG YFVPAF+GL
Sbjct: 310 IGDQKPV-YALEGSIAVTGSLVQWMRDQMGLIKSAAEIETLASSVEDNGGAYFVPAFSGL 369

Query: 368 FAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDGGEKGEVKNEKGE 427
           FAP+WR DARGV  G+TR+ +KAHIARAVLE+  +Q +++ D+M KD G +         
Sbjct: 370 FAPYWRSDARGVIAGLTRYVTKAHIARAVLEATAWQTREITDAMTKDSGVELAA------ 429

Query: 428 FLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTKDEIFDSG 487
             L+VDGG T NNLLMQ  +D L +PVVRP   ETT LGAAYAAGLAVG WT  E  D  
Sbjct: 430 --LKVDGGMTSNNLLMQTLSDFLDAPVVRPMVAETTCLGAAYAAGLAVGFWTNTE--DLR 489

Query: 488 ERVKSATTFRPVLEDESRKKKVESWCKAVSRT 516
              + A  + P +  E+R ++ +SW KAV RT
Sbjct: 490 ANWRRAAEWTPNMAAETRDREYKSWLKAVERT 504

BLAST of Spg014781 vs. ExPASy Swiss-Prot
Match: A5CS23 (Glycerol kinase OS=Clavibacter michiganensis subsp. michiganensis (strain NCPPB 382) OX=443906 GN=glpK PE=3 SV=1)

HSP 1 Score: 540.0 bits (1390), Expect = 2.9e-152
Identity = 262/516 (50.78%), Postives = 365/516 (70.74%), Query Frame = 0

Query: 6   DAFVGSIDQGTTSTRFIIYDRRSRPIGSHQVEFTQFYPEAGWVEHNPVEILESVKVCMAK 65
           + ++ +IDQGTTSTR I++D     + + Q+E  Q +P AGWVEH+P+EI  + +  + +
Sbjct: 3   EKYIVAIDQGTTSTRAIVFDHSGSIVSTGQLEHEQIFPRAGWVEHDPMEIWRNTREVIGQ 62

Query: 66  ALDKATAAGYNVDKGLKAIGLTNQRETTLVWSKSTGSPLHNAIVWMDVRTSSICRKLEKE 125
           AL KA    ++V+    A+G+TNQRET +VW ++TG P++NAIVW D RT  I  +L  +
Sbjct: 63  ALSKADITRHDVE----AVGITNQRETAVVWDRTTGKPVYNAIVWQDTRTQKIVDRLAAD 122

Query: 126 LSGGRTHFGDTCGLPISTYFSAVKLLWLMENVEDVRESVKKGDALFGTIDTWLIWNLTGG 185
             GG   F  T GLP++TYFS  K++W++ENV+  RE  + G+ +FGT DTW++WNLTGG
Sbjct: 123 --GGVERFKPTVGLPLATYFSGTKIVWILENVDGAREKAEAGELMFGTTDTWVLWNLTGG 182

Query: 186 VNGGLHVTDVSNASRTMLMNLKTLDWDKPTLQTLGIPAQILPKIVSNSEVIGKIAQGWPI 245
            +GG+HVTDV+NASRT+ M+L+TL WD   L+   +P  +LP+I S+SEV G++     +
Sbjct: 183 TDGGVHVTDVTNASRTLFMDLETLQWDDEILKAFDVPRSMLPEIKSSSEVYGQVESSSLL 242

Query: 246 TGVPISGCLGDQHAAMLGQAC-RKGEAKSTYGTGAFILLNTGEEVVHSKHGLLTTLAFKL 305
             VPI+G LGDQ AA  GQA   +GE+K+TYGTG F++ NTG +++HS++GLLTTL +KL
Sbjct: 243 REVPIAGILGDQQAATFGQAAFDQGESKNTYGTGNFLIFNTGTDIIHSQNGLLTTLGYKL 302

Query: 306 GQDAPTNYALEGSIAIAGAAVQWLRDGLGIISSAQEIEELASQVKSTGGVYFVPAFNGLF 365
           G   P +YALEGSIA+ G+ VQW+RD LG++SSA EIE LA+ V+  GGVYFVPAF+GLF
Sbjct: 303 GDQEP-HYALEGSIAVTGSLVQWMRDNLGLVSSAAEIETLAATVEDNGGVYFVPAFSGLF 362

Query: 366 APWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDGGEKGEVKNEKGEF 425
           AP+WR DARG  +G+TR+ +K HIARA LE+  FQ ++VLD+++ D G       +  E 
Sbjct: 363 APYWRSDARGALVGLTRYVNKGHIARAALEATAFQTREVLDAVNADSGV------DLTE- 422

Query: 426 LLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTKDEIFDSGE 485
            L+VDGG   NNLLMQ QAD+LG PVVRP   ETTALGAAYAAGLAVG W   ++ D  +
Sbjct: 423 -LKVDGGMIANNLLMQFQADILGVPVVRPVVAETTALGAAYAAGLAVGFW--KDLDDLRQ 482

Query: 486 RVKSATTFRPVLEDESRKKKVESWCKAVSRTFDLAD 521
             +  + + P ++D  R++++  W KAV++TFD  D
Sbjct: 483 NWQEDSRWTPDMDDAERERQLRLWKKAVTKTFDWVD 501

BLAST of Spg014781 vs. ExPASy Swiss-Prot
Match: A9WS93 (Glycerol kinase OS=Renibacterium salmoninarum (strain ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235) OX=288705 GN=glpK PE=3 SV=1)

HSP 1 Score: 538.9 bits (1387), Expect = 6.4e-152
Identity = 265/514 (51.56%), Postives = 356/514 (69.26%), Query Frame = 0

Query: 8   FVGSIDQGTTSTRFIIYDRRSRPIGSHQVEFTQFYPEAGWVEHNPVEILESVKVCMAKAL 67
           +V +IDQGTTS+R II+D     + + Q+E  Q +P+AGWVEHN  EI  + +  +  AL
Sbjct: 4   YVIAIDQGTTSSRAIIFDHDGNIVSTGQLEHEQIFPKAGWVEHNATEIWNNTREVIGTAL 63

Query: 68  DKATAAGYNVDKGLKAIGLTNQRETTLVWSKSTGSPLHNAIVWMDVRTSSICRKLEKELS 127
            KA    +++     A+G+TNQRET +VW K+TG P++NAIVW D RT SI  +L   + 
Sbjct: 64  SKANLTRHDI----AAVGITNQRETAVVWDKTTGEPVYNAIVWQDTRTQSIVDEL--AVG 123

Query: 128 GGRTHFGDTCGLPISTYFSAVKLLWLMENVEDVRESVKKGDALFGTIDTWLIWNLTGGVN 187
           GG   F D  GLP++TYFS  K+ W+++NV+  RE    GD LFG  D+W+ WNLTGGV+
Sbjct: 124 GGVERFKDKVGLPLATYFSGTKIKWILDNVDGARERAAAGDLLFGNTDSWVTWNLTGGVD 183

Query: 188 GGLHVTDVSNASRTMLMNLKTLDWDKPTLQTLGIPAQILPKIVSNSEVIGKIAQGWPITG 247
           GG+H+TDV+NASRTM M+L TL WD+  L   G+PA +LP I S+SEV G +     +  
Sbjct: 184 GGVHITDVTNASRTMFMDLATLSWDQEILDAFGVPASMLPTIKSSSEVYGTVHTSQLLRE 243

Query: 248 VPISGCLGDQHAAMLGQAC-RKGEAKSTYGTGAFILLNTGEEVVHSKHGLLTTLAFKLGQ 307
           VP++G LGDQ AA  GQA    GEAK+TYGTG F++ NTGEE+VHSK+GLLTT+ +KLG 
Sbjct: 244 VPVAGILGDQQAATFGQAAFNTGEAKNTYGTGCFLIFNTGEEIVHSKNGLLTTVGYKLG- 303

Query: 308 DAPTNYALEGSIAIAGAAVQWLRDGLGIISSAQEIEELASQVKSTGGVYFVPAFNGLFAP 367
           DAPT+YALEGSIA+AG+ +QWLRD LG+ISSA E+EELA+ V+  GGVY VPAF+GLFAP
Sbjct: 304 DAPTHYALEGSIAVAGSLIQWLRDTLGMISSAPEVEELAASVEDNGGVYIVPAFSGLFAP 363

Query: 368 WWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDGGEKGEVKNEKGEFLL 427
           +WR DARG  +G+TR+ ++ HIARA LE+  FQ ++VLD+++ D G             L
Sbjct: 364 YWRSDARGAIVGMTRYVNRNHIARAALEATAFQTREVLDAVNADSGVPLTE--------L 423

Query: 428 RVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTKDEIFDSGERV 487
           +VDGG   N+ LMQ QAD+LG PV+RP   ETTALGAAYAAGLAVG W  +++ +     
Sbjct: 424 KVDGGMVANDALMQFQADILGVPVIRPKVTETTALGAAYAAGLAVGFW--NDLGELSSNW 483

Query: 488 KSATTFRPVLEDESRKKKVESWCKAVSRTFDLAD 521
                + P +E++ R +++  W KAV+++ D  D
Sbjct: 484 AEDKRWEPAMEEDERARQLRLWKKAVTKSMDWVD 500

BLAST of Spg014781 vs. ExPASy TrEMBL
Match: A0A1S3CF92 (Glycerol kinase OS=Cucumis melo OX=3656 GN=LOC103499817 PE=3 SV=1)

HSP 1 Score: 1012.7 bits (2617), Expect = 5.6e-292
Identity = 498/523 (95.22%), Postives = 510/523 (97.51%), Query Frame = 0

Query: 1   MAKAEDAFVGSIDQGTTSTRFIIYDRRSRPIGSHQVEFTQFYPEAGWVEHNPVEILESVK 60
           MAKAED FVGSIDQGTTSTRFIIYDR SRPIGSHQ EFTQFYPEAGWVEH+PVEILESVK
Sbjct: 1   MAKAEDVFVGSIDQGTTSTRFIIYDRHSRPIGSHQAEFTQFYPEAGWVEHDPVEILESVK 60

Query: 61  VCMAKALDKATAAGYNVDKGLKAIGLTNQRETTLVWSKSTGSPLHNAIVWMDVRTSSICR 120
           VCMAKALDKATAAGYNVD GLKAIGLTNQRETTLVWSKS+GSPLHNAIVWMD RTSSICR
Sbjct: 61  VCMAKALDKATAAGYNVDNGLKAIGLTNQRETTLVWSKSSGSPLHNAIVWMDARTSSICR 120

Query: 121 KLEKELSGGRTHFGDTCGLPISTYFSAVKLLWLMENVEDVRESVKKGDALFGTIDTWLIW 180
           KLEKELSGGRTHF DTCGLPISTYFSAVKL+WLMENVE VRES++KGDALFGTIDTWLIW
Sbjct: 121 KLEKELSGGRTHFVDTCGLPISTYFSAVKLIWLMENVEAVRESIEKGDALFGTIDTWLIW 180

Query: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLDWDKPTLQTLGIPAQILPKIVSNSEVIGKIA 240
           NLTGGVNGGLHVTDVSNASRTMLMNLKTLDWDKPTL+TLGIPAQILPKIVSNSEVIGKI+
Sbjct: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLDWDKPTLETLGIPAQILPKIVSNSEVIGKIS 240

Query: 241 QGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVHSKHGLLTTL 300
           QGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVV SKHGLLTTL
Sbjct: 241 QGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTL 300

Query: 301 AFKLGQDAPTNYALEGSIAIAGAAVQWLRDGLGIISSAQEIEELASQVKSTGGVYFVPAF 360
           AFKLG+DAPTNYALEGSIAIAGAAVQWLRD LGIISSAQEIEELA QV+STGGVYFVPAF
Sbjct: 301 AFKLGRDAPTNYALEGSIAIAGAAVQWLRDSLGIISSAQEIEELALQVQSTGGVYFVPAF 360

Query: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDGGEKGEVKNE 420
           NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKD GEKGEVKNE
Sbjct: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAGEKGEVKNE 420

Query: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTKDEIF 480
           KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVG+W+KDEIF
Sbjct: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGIWSKDEIF 480

Query: 481 DSGERVKSATTFRPVLEDESRKKKVESWCKAVSRTFDLADLSL 524
           DSGERVKSATTF PVLE+E RKKKVESWCKAVSRTFDLADLSL
Sbjct: 481 DSGERVKSATTFYPVLEEELRKKKVESWCKAVSRTFDLADLSL 523

BLAST of Spg014781 vs. ExPASy TrEMBL
Match: A0A5A7UVL8 (Glycerol kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G002070 PE=3 SV=1)

HSP 1 Score: 1010.7 bits (2612), Expect = 2.1e-291
Identity = 497/523 (95.03%), Postives = 509/523 (97.32%), Query Frame = 0

Query: 1   MAKAEDAFVGSIDQGTTSTRFIIYDRRSRPIGSHQVEFTQFYPEAGWVEHNPVEILESVK 60
           MAKAED FVGSIDQGTTSTRFIIYDR SRPIGSHQ EFTQFYPEAGWVEH+PVEILESVK
Sbjct: 1   MAKAEDVFVGSIDQGTTSTRFIIYDRHSRPIGSHQAEFTQFYPEAGWVEHDPVEILESVK 60

Query: 61  VCMAKALDKATAAGYNVDKGLKAIGLTNQRETTLVWSKSTGSPLHNAIVWMDVRTSSICR 120
           VCMAKALDKATAAGYNVD GLKAIGLTNQRETTLVWSKS+GSPLHNAIVWMD RTSSICR
Sbjct: 61  VCMAKALDKATAAGYNVDNGLKAIGLTNQRETTLVWSKSSGSPLHNAIVWMDARTSSICR 120

Query: 121 KLEKELSGGRTHFGDTCGLPISTYFSAVKLLWLMENVEDVRESVKKGDALFGTIDTWLIW 180
           KLEKELSGGRTHF DTCGLPISTYFSAVKL+WLMENVE VRES++KGDALFGTIDTWLIW
Sbjct: 121 KLEKELSGGRTHFVDTCGLPISTYFSAVKLIWLMENVEAVRESIEKGDALFGTIDTWLIW 180

Query: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLDWDKPTLQTLGIPAQILPKIVSNSEVIGKIA 240
           NLTGGVNGGLHVTDVSNASRTMLMNLKTLDWDKPTL+TLGIPAQILPKIVSNSEVIGKI+
Sbjct: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLDWDKPTLETLGIPAQILPKIVSNSEVIGKIS 240

Query: 241 QGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVHSKHGLLTTL 300
           QGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVV SKHGLLTTL
Sbjct: 241 QGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTL 300

Query: 301 AFKLGQDAPTNYALEGSIAIAGAAVQWLRDGLGIISSAQEIEELASQVKSTGGVYFVPAF 360
           AFKLG+DAPTNYALEGSIAIAGAAVQWLRD LGIISSAQEIEELA QV+STGGVYFVPAF
Sbjct: 301 AFKLGRDAPTNYALEGSIAIAGAAVQWLRDSLGIISSAQEIEELALQVQSTGGVYFVPAF 360

Query: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDGGEKGEVKNE 420
           NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKD GEKGEVKNE
Sbjct: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAGEKGEVKNE 420

Query: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTKDEIF 480
           KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVG+W+KDEIF
Sbjct: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGIWSKDEIF 480

Query: 481 DSGERVKSATTFRPVLEDESRKKKVESWCKAVSRTFDLADLSL 524
           DSGERVK ATTF PVLE+E RKKKVESWCKAVSRTFDLADLSL
Sbjct: 481 DSGERVKRATTFYPVLEEELRKKKVESWCKAVSRTFDLADLSL 523

BLAST of Spg014781 vs. ExPASy TrEMBL
Match: A0A0A0KA32 (Glycerol kinase OS=Cucumis sativus OX=3659 GN=Csa_7G432280 PE=3 SV=1)

HSP 1 Score: 1003.4 bits (2593), Expect = 3.4e-289
Identity = 495/523 (94.65%), Postives = 507/523 (96.94%), Query Frame = 0

Query: 1   MAKAEDAFVGSIDQGTTSTRFIIYDRRSRPIGSHQVEFTQFYPEAGWVEHNPVEILESVK 60
           MAKAED FVGSIDQGTTS+RFIIYDR SRPIGSHQ EFTQFYPEAGWVEH+PVEILESVK
Sbjct: 1   MAKAEDVFVGSIDQGTTSSRFIIYDRHSRPIGSHQAEFTQFYPEAGWVEHDPVEILESVK 60

Query: 61  VCMAKALDKATAAGYNVDKGLKAIGLTNQRETTLVWSKSTGSPLHNAIVWMDVRTSSICR 120
           VCMAKALDKATAAGYNVD GLKAIGLTNQRETTLVWSKSTG PLH+AIVWMD RTSSICR
Sbjct: 61  VCMAKALDKATAAGYNVDNGLKAIGLTNQRETTLVWSKSTGFPLHHAIVWMDARTSSICR 120

Query: 121 KLEKELSGGRTHFGDTCGLPISTYFSAVKLLWLMENVEDVRESVKKGDALFGTIDTWLIW 180
           KLEKELSGGRTHF DTCGLPISTYFSAVKL+WLMENVE VRES+KK DALFGTIDTWLIW
Sbjct: 121 KLEKELSGGRTHFVDTCGLPISTYFSAVKLIWLMENVEAVRESIKKEDALFGTIDTWLIW 180

Query: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLDWDKPTLQTLGIPAQILPKIVSNSEVIGKIA 240
           NLTGGVNGGLHVTDVSNASRTMLMNLKTLDWDKPTL+TLGIPAQILPKIVSNSEVIGKI+
Sbjct: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLDWDKPTLETLGIPAQILPKIVSNSEVIGKIS 240

Query: 241 QGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVHSKHGLLTTL 300
           QGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVV SKHGLLTTL
Sbjct: 241 QGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTL 300

Query: 301 AFKLGQDAPTNYALEGSIAIAGAAVQWLRDGLGIISSAQEIEELASQVKSTGGVYFVPAF 360
           AFKLG DAPTNYALEGSIAIAGAAVQWLRD LGIISSAQEIE+LASQV+STGGVYFVPAF
Sbjct: 301 AFKLGPDAPTNYALEGSIAIAGAAVQWLRDSLGIISSAQEIEKLASQVQSTGGVYFVPAF 360

Query: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDGGEKGEVKNE 420
           NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKD  EKGEVKNE
Sbjct: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAVEKGEVKNE 420

Query: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTKDEIF 480
           KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVG+W+KDEIF
Sbjct: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGIWSKDEIF 480

Query: 481 DSGERVKSATTFRPVLEDESRKKKVESWCKAVSRTFDLADLSL 524
           DSGERVKSATTF PVLE+E RKKKVESWCKAVSRTFDLADLSL
Sbjct: 481 DSGERVKSATTFYPVLEEELRKKKVESWCKAVSRTFDLADLSL 523

BLAST of Spg014781 vs. ExPASy TrEMBL
Match: A0A6J1GPN0 (Glycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111456345 PE=3 SV=1)

HSP 1 Score: 993.4 bits (2567), Expect = 3.5e-286
Identity = 488/523 (93.31%), Postives = 505/523 (96.56%), Query Frame = 0

Query: 1   MAKAEDAFVGSIDQGTTSTRFIIYDRRSRPIGSHQVEFTQFYPEAGWVEHNPVEILESVK 60
           MAKAED FVGSIDQGTTSTRFIIYDR SRPIGSHQVEFTQFYPEAGWVEH+P+EILESV+
Sbjct: 1   MAKAEDVFVGSIDQGTTSTRFIIYDRCSRPIGSHQVEFTQFYPEAGWVEHDPMEILESVR 60

Query: 61  VCMAKALDKATAAGYNVDKGLKAIGLTNQRETTLVWSKSTGSPLHNAIVWMDVRTSSICR 120
           VCMAKA+DKATAAGYNVD GLKAIGLTNQRETTLVWSKSTGSPLHNAIVWMD RTS+ICR
Sbjct: 61  VCMAKAVDKATAAGYNVDNGLKAIGLTNQRETTLVWSKSTGSPLHNAIVWMDARTSAICR 120

Query: 121 KLEKELSGGRTHFGDTCGLPISTYFSAVKLLWLMENVEDVRESVKKGDALFGTIDTWLIW 180
           KLE ELSGGRTHF  TCGLPISTYFSAVKLLWLMENV+DV ES+KKGDALFGTIDTWLIW
Sbjct: 121 KLENELSGGRTHFVGTCGLPISTYFSAVKLLWLMENVKDVNESIKKGDALFGTIDTWLIW 180

Query: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLDWDKPTLQTLGIPAQILPKIVSNSEVIGKIA 240
           NLTGGVNGGLHVTDVSNASRTMLMNLKTL+WDKPTL+TLGIPAQILPKIVSNSEVIGKI 
Sbjct: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLEWDKPTLETLGIPAQILPKIVSNSEVIGKIV 240

Query: 241 QGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVHSKHGLLTTL 300
           QGWPITGVPISGCLGDQHAAMLGQACRKGEAKST+GTGAFILLNTGEEVV SKHGLLTTL
Sbjct: 241 QGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTFGTGAFILLNTGEEVVQSKHGLLTTL 300

Query: 301 AFKLGQDAPTNYALEGSIAIAGAAVQWLRDGLGIISSAQEIEELASQVKSTGGVYFVPAF 360
           AFKLG+DA  NYALEGSIAIAGAAVQWLRD LGIISSA+EIEELASQVKSTGG+YFVPAF
Sbjct: 301 AFKLGKDARANYALEGSIAIAGAAVQWLRDSLGIISSAKEIEELASQVKSTGGIYFVPAF 360

Query: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDGGEKGEVKNE 420
           NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKD  EKGEVKNE
Sbjct: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAREKGEVKNE 420

Query: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTKDEIF 480
           KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWT+DEIF
Sbjct: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTEDEIF 480

Query: 481 DSGERVKSATTFRPVLEDESRKKKVESWCKAVSRTFDLADLSL 524
            +GERV+SATTF PVLE+E RKKKVESWCKAVSRTFDLADLSL
Sbjct: 481 SAGERVESATTFHPVLEEELRKKKVESWCKAVSRTFDLADLSL 523

BLAST of Spg014781 vs. ExPASy TrEMBL
Match: A0A6J1JTB9 (Glycerol kinase OS=Cucurbita maxima OX=3661 GN=LOC111488147 PE=3 SV=1)

HSP 1 Score: 992.3 bits (2564), Expect = 7.9e-286
Identity = 486/523 (92.93%), Postives = 504/523 (96.37%), Query Frame = 0

Query: 1   MAKAEDAFVGSIDQGTTSTRFIIYDRRSRPIGSHQVEFTQFYPEAGWVEHNPVEILESVK 60
           MAK ED FVGSIDQGTTSTRFIIYDR SRPIGSHQVEFTQFYPEAGWVEH+P+EILESV+
Sbjct: 1   MAKVEDVFVGSIDQGTTSTRFIIYDRCSRPIGSHQVEFTQFYPEAGWVEHDPMEILESVR 60

Query: 61  VCMAKALDKATAAGYNVDKGLKAIGLTNQRETTLVWSKSTGSPLHNAIVWMDVRTSSICR 120
           VCMAKA+DKATAAGYNVD GLKAIGLTNQRETTLVWSKSTGSPLHNAIVWMD RTS+ICR
Sbjct: 61  VCMAKAVDKATAAGYNVDNGLKAIGLTNQRETTLVWSKSTGSPLHNAIVWMDARTSAICR 120

Query: 121 KLEKELSGGRTHFGDTCGLPISTYFSAVKLLWLMENVEDVRESVKKGDALFGTIDTWLIW 180
           KLE ELSGGRTHF  TCGLPISTYFSA+KLLWLMENV+DV ES+KKGDALFGTIDTWLIW
Sbjct: 121 KLENELSGGRTHFVGTCGLPISTYFSAMKLLWLMENVKDVNESIKKGDALFGTIDTWLIW 180

Query: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLDWDKPTLQTLGIPAQILPKIVSNSEVIGKIA 240
           NLTGGVNGGLHVTDVSNASRTMLMNLKTL+WDKPTL+TLGIPAQILPKIVSNSEVIGKI 
Sbjct: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLEWDKPTLETLGIPAQILPKIVSNSEVIGKIV 240

Query: 241 QGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVHSKHGLLTTL 300
           QGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVV SKHGLLTTL
Sbjct: 241 QGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVQSKHGLLTTL 300

Query: 301 AFKLGQDAPTNYALEGSIAIAGAAVQWLRDGLGIISSAQEIEELASQVKSTGGVYFVPAF 360
           AFKLG+DA  NYALEGSIAIAGAAVQWLRD LGIISSA+EIEELASQVKSTGG+YFVPAF
Sbjct: 301 AFKLGKDARANYALEGSIAIAGAAVQWLRDSLGIISSAKEIEELASQVKSTGGIYFVPAF 360

Query: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDGGEKGEVKNE 420
           NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKD GEKGE KNE
Sbjct: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDAGEKGEAKNE 420

Query: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTKDEIF 480
           KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRP+DIETTALGAAYAAGLAVGVWT+DEIF
Sbjct: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPSDIETTALGAAYAAGLAVGVWTEDEIF 480

Query: 481 DSGERVKSATTFRPVLEDESRKKKVESWCKAVSRTFDLADLSL 524
            +GERV+SATTF PVLE+E RKKKVESWCKAVSRTFDLADLSL
Sbjct: 481 TAGERVESATTFHPVLEEELRKKKVESWCKAVSRTFDLADLSL 523

BLAST of Spg014781 vs. TAIR 10
Match: AT1G80460.1 (Actin-like ATPase superfamily protein )

HSP 1 Score: 891.3 bits (2302), Expect = 3.6e-259
Identity = 428/523 (81.84%), Postives = 478/523 (91.40%), Query Frame = 0

Query: 1   MAKAEDAFVGSIDQGTTSTRFIIYDRRSRPIGSHQVEFTQFYPEAGWVEHNPVEILESVK 60
           MAK E+ F+GSIDQGTTSTRFIIYD  +RP+ SHQVEFTQFYPEAGWVEH+P+EILESVK
Sbjct: 1   MAK-ENGFIGSIDQGTTSTRFIIYDHDARPVASHQVEFTQFYPEAGWVEHDPMEILESVK 60

Query: 61  VCMAKALDKATAAGYNVDKGLKAIGLTNQRETTLVWSKSTGSPLHNAIVWMDVRTSSICR 120
           VC+AKALDKATA G+NVD GLKAIGLT+QRETT+VWSKSTG PLH AIVWMD RTSSICR
Sbjct: 61  VCIAKALDKATADGHNVDGGLKAIGLTDQRETTVVWSKSTGLPLHKAIVWMDARTSSICR 120

Query: 121 KLEKELSGGRTHFGDTCGLPISTYFSAVKLLWLMENVEDVRESVKKGDALFGTIDTWLIW 180
           +LEKELSGGR+HF ++CGLPISTYFSA+KLLWLMENV+DV++++KKGDA+FGTIDTWLIW
Sbjct: 121 RLEKELSGGRSHFVESCGLPISTYFSAMKLLWLMENVDDVKDAIKKGDAIFGTIDTWLIW 180

Query: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLDWDKPTLQTLGIPAQILPKIVSNSEVIGKIA 240
           N+TGG+NGGLHVTDV+NASRTMLMNLKTL WD+ TL+TLGIPA+ILPKIVSNSEVIG+I 
Sbjct: 181 NMTGGINGGLHVTDVTNASRTMLMNLKTLSWDQDTLKTLGIPAEILPKIVSNSEVIGEIC 240

Query: 241 QGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVHSKHGLLTTL 300
           +GWPI G+ I+GCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGE  + S HGLLTTL
Sbjct: 241 KGWPIPGIKIAGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEVPIKSGHGLLTTL 300

Query: 301 AFKLGQDAPTNYALEGSIAIAGAAVQWLRDGLGIISSAQEIEELASQVKSTGGVYFVPAF 360
           A+KLG  A TNYALEGSIAIAGAAVQWLRD LGII SA EIE+LA+ V STGGVYFVPAF
Sbjct: 301 AYKLGPQAQTNYALEGSIAIAGAAVQWLRDSLGIIKSASEIEDLAAMVDSTGGVYFVPAF 360

Query: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDGGEKGEVKNE 420
           NGLFAPWWR+DARGVCIGITRFT+K+HIARAVLESMCFQVKDVLDSM+KD GEKG + N 
Sbjct: 361 NGLFAPWWREDARGVCIGITRFTNKSHIARAVLESMCFQVKDVLDSMNKDAGEKGSLNNG 420

Query: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTKDEIF 480
           KGEFLLRVDGGAT NNLLMQIQADL+GSPVVRP DIETTALGAAYAAGLAVG W + +IF
Sbjct: 421 KGEFLLRVDGGATANNLLMQIQADLMGSPVVRPVDIETTALGAAYAAGLAVGFWKEADIF 480

Query: 481 DSGERVKSATTFRPVLEDESRKKKVESWCKAVSRTFDLADLSL 524
           +SGE+ K++  FRP +E+  RKKKV SWCKAV RTFDLADLS+
Sbjct: 481 ESGEKAKNSKVFRPAMEEGIRKKKVASWCKAVERTFDLADLSI 522

BLAST of Spg014781 vs. TAIR 10
Match: AT1G80460.2 (Actin-like ATPase superfamily protein )

HSP 1 Score: 801.2 bits (2068), Expect = 4.9e-232
Identity = 391/523 (74.76%), Postives = 440/523 (84.13%), Query Frame = 0

Query: 1   MAKAEDAFVGSIDQGTTSTRFIIYDRRSRPIGSHQVEFTQFYPEAGWVEHNPVEILESVK 60
           MAK E+ F+GSIDQGTTSTRFIIYD  +RP+ SHQVEFTQFYPEAGWVEH+P+EILESVK
Sbjct: 1   MAK-ENGFIGSIDQGTTSTRFIIYDHDARPVASHQVEFTQFYPEAGWVEHDPMEILESVK 60

Query: 61  VCMAKALDKATAAGYNVDKGLKAIGLTNQRETTLVWSKSTGSPLHNAIVWMDVRTSSICR 120
           VC+AKALDKATA G+NVD GLKAIGLT+QRETT+VWSKSTG PLH AIVWMD RTSSICR
Sbjct: 61  VCIAKALDKATADGHNVDGGLKAIGLTDQRETTVVWSKSTGLPLHKAIVWMDARTSSICR 120

Query: 121 KLEKELSGGRTHFGDTCGLPISTYFSAVKLLWLMENVEDVRESVKKGDALFGTIDTWLIW 180
           +LEKELSGGR+HF ++CGLPISTYFSA+KLLWLMENV+DV++++KKGDA+FGTIDTWLIW
Sbjct: 121 RLEKELSGGRSHFVESCGLPISTYFSAMKLLWLMENVDDVKDAIKKGDAIFGTIDTWLIW 180

Query: 181 NLTGGVNGGLHVTDVSNASRTMLMNLKTLDWDKPTLQTLGIPAQILPKIVSNSEVIGKIA 240
           N+TGG+NGGLHVTDV+NASRTMLMNLKTL WD+ TL+TLGIPA+ILPKIVSNSEVIG+I 
Sbjct: 181 NMTGGINGGLHVTDVTNASRTMLMNLKTLSWDQDTLKTLGIPAEILPKIVSNSEVIGEIC 240

Query: 241 QGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEVVHSKHGLLTTL 300
           +GWPI G+ I+GCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGE  + S HGLLTTL
Sbjct: 241 KGWPIPGIKIAGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEVPIKSGHGLLTTL 300

Query: 301 AFKLGQDAPTNYALEGSIAIAGAAVQWLRDGLGIISSAQEIEELASQVKSTGGVYFVPAF 360
           A+KLG  A TNYALEGSIAIAGAAVQWLRD LGII SA EIE+LA+ V STGGVYFVPAF
Sbjct: 301 AYKLGPQAQTNYALEGSIAIAGAAVQWLRDSLGIIKSASEIEDLAAMVDSTGGVYFVPAF 360

Query: 361 NGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHKDGGEKGEVKNE 420
           NGLFAPWWR+DARGVCIGITRFT+K+HIARAVLESMCFQVKDVLDSM+KD GEKG + N 
Sbjct: 361 NGLFAPWWREDARGVCIGITRFTNKSHIARAVLESMCFQVKDVLDSMNKDAGEKGSLNNG 420

Query: 421 KGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGVWTKDEIF 480
           KGEFLLRVD                                        AVG W + +IF
Sbjct: 421 KGEFLLRVD----------------------------------------AVGFWKEADIF 480

Query: 481 DSGERVKSATTFRPVLEDESRKKKVESWCKAVSRTFDLADLSL 524
           +SGE+ K++  FRP +E+  RKKKV SWCKAV RTFDLADLS+
Sbjct: 481 ESGEKAKNSKVFRPAMEEGIRKKKVASWCKAVERTFDLADLSI 482

BLAST of Spg014781 vs. TAIR 10
Match: AT4G30310.2 (FGGY family of carbohydrate kinase )

HSP 1 Score: 75.5 bits (184), Expect = 1.4e-13
Identity = 114/535 (21.31%), Postives = 209/535 (39.07%), Query Frame = 0

Query: 4   AEDAFVGSIDQGTTSTRFIIYDRRSRPIGSHQVEFTQFYPEAGWVEHNPVEILESVKVCM 63
           +   F+G +D GT S R  ++D   + +GS      Q + +   +E +  +I  +V   +
Sbjct: 11  SRSVFLG-VDVGTGSARAGLFDDNGKLLGS-ATSPIQIWKDGDCIEQSSTDIWHAVCAAV 70

Query: 64  AKALDKATAAGYNVDKGLKAIGLTNQRETTLVWSKSTGSPL---------HNAIVWMDVR 123
             A   A  +    D  +K IG       +LV   + GSP+          N IVWMD R
Sbjct: 71  KSACSLANVS----DVEVKGIGFA--ATCSLVAVDAEGSPVTVSWSGDSRRNIIVWMDHR 130

Query: 124 TSSICRKLEKELSGGRTHFGDTCGLPISTYFSAVKLLWLMENVEDVRESVKKGDALFGTI 183
                 K  + ++   +     CG  +S      KLLW+ EN+++    V K    +  +
Sbjct: 131 AV----KQAERINSFNSPVLQYCGGGVSPEMEPPKLLWVKENLKESWSMVYK----WMDL 190

Query: 184 DTWLIWNLTGGVNGGLHVT----------DVSNASRTMLMNLKTLDWDKPTLQTLGI--- 243
             WL +  TG     L  T           +   +     +++   WD    + +G+   
Sbjct: 191 SDWLSYRATGDDTRSLCTTVCKWTYLGHAHMHQMTEKASRDMEACGWDDEFWEEIGLGDL 250

Query: 244 ----PAQILPKIVSNSEVIGK------IAQGWPITGVPISGCLGDQHAAMLGQACRKGEA 303
                A+I   +      +G         +   + G P+   L D HA  +G    K E+
Sbjct: 251 VDGHHAKIGRSVAFPGHPLGNGLTATAAKELGLLAGTPVGTSLIDAHAGGVGVMESKLES 310

Query: 304 KSTYGTG------AFILLNTGEEVVH---SKHGLLTTLAFKLGQDA--PTNYALEGSIAI 363
            S           + ++L  G    H   S+  L     +     A  P  +  EG  + 
Sbjct: 311 DSLTKESDVDTLCSRMVLVCGTSTCHMAVSREKLFIPGVWGPFWSAMVPEYWLTEGGQSA 370

Query: 364 AGAAVQWLRDG------LGIISSAQEI--------------EELASQVKS--TGGVYFVP 423
            GA +  + +       L   +++Q++              E+ +S   S  T  ++ +P
Sbjct: 371 TGALLDHIIENHVASPRLANRAASQKVSVFELLNNILKTMAEDTSSPFISALTSDMHILP 430

Query: 424 AFNGLFAPWWRDDARGVCIGITRFTSKAHIA---RAVLESMCFQVKDVLDSMHKDGGEKG 471
            F+G  +P    +++GV  G++  TS+  +A    A ++ + +  + +++  +  G    
Sbjct: 431 DFHGNRSPVADPNSKGVIFGMSLDTSEKQLALLYLATIQGIAYGTRHIVEHCNTHG---- 490

BLAST of Spg014781 vs. TAIR 10
Match: AT4G30310.3 (FGGY family of carbohydrate kinase )

HSP 1 Score: 48.9 bits (115), Expect = 1.4e-05
Identity = 32/123 (26.02%), Postives = 63/123 (51.22%), Query Frame = 0

Query: 351 TGGVYFVPAFNGLFAPWWRDDARGVCIGITRFTSKAHIA---RAVLESMCFQVKDVLDSM 410
           T  ++ +P F+G  +P    +++GV  G++  TS+  +A    A ++ + +  + +++  
Sbjct: 279 TSDMHILPDFHGNRSPVADPNSKGVIFGMSLDTSEKQLALLYLATIQGIAYGTRHIVEHC 338

Query: 411 HKDGGEKGEVKNEKGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAA 470
           +  G         K + LL   GG + N L +Q  AD++G P++ P + E+  LGAA   
Sbjct: 339 NTHG--------HKIDTLLAC-GGLSKNPLFIQEHADIVGCPIILPRESESVLLGAAILG 392

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008461141.11.2e-29195.22PREDICTED: glycerol kinase [Cucumis melo][more]
KAA0058737.14.4e-29195.03glycerol kinase [Cucumis melo var. makuwa][more]
XP_038899608.16.4e-29094.65glycerol kinase [Benincasa hispida] >XP_038899609.1 glycerol kinase [Benincasa h... [more]
XP_004135863.17.0e-28994.65glycerol kinase [Cucumis sativus] >KGN45247.1 hypothetical protein Csa_015818 [C... [more]
KAG6575609.12.5e-28693.50Glycerol kinase, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q9M8L45.1e-25881.84Glycerol kinase OS=Arabidopsis thaliana OX=3702 GN=GLPK PE=1 SV=1[more]
Q54VT81.2e-15349.27Probable glycerol kinase OS=Dictyostelium discoideum OX=44689 GN=gk PE=3 SV=1[more]
Q828K52.9e-15252.73Glycerol kinase 1 OS=Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / J... [more]
A5CS232.9e-15250.78Glycerol kinase OS=Clavibacter michiganensis subsp. michiganensis (strain NCPPB ... [more]
A9WS936.4e-15251.56Glycerol kinase OS=Renibacterium salmoninarum (strain ATCC 33209 / DSM 20767 / J... [more]
Match NameE-valueIdentityDescription
A0A1S3CF925.6e-29295.22Glycerol kinase OS=Cucumis melo OX=3656 GN=LOC103499817 PE=3 SV=1[more]
A0A5A7UVL82.1e-29195.03Glycerol kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G0020... [more]
A0A0A0KA323.4e-28994.65Glycerol kinase OS=Cucumis sativus OX=3659 GN=Csa_7G432280 PE=3 SV=1[more]
A0A6J1GPN03.5e-28693.31Glycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111456345 PE=3 SV=1[more]
A0A6J1JTB97.9e-28692.93Glycerol kinase OS=Cucurbita maxima OX=3661 GN=LOC111488147 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G80460.13.6e-25981.84Actin-like ATPase superfamily protein [more]
AT1G80460.24.9e-23274.76Actin-like ATPase superfamily protein [more]
AT4G30310.21.4e-1321.31FGGY family of carbohydrate kinase [more]
AT4G30310.31.4e-0526.02FGGY family of carbohydrate kinase [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.420.40coord: 4..264
e-value: 1.9E-88
score: 298.5
NoneNo IPR availableGENE3D3.30.420.40coord: 265..518
e-value: 9.4E-91
score: 305.3
NoneNo IPR availablePANTHERPTHR10196SUGAR KINASEcoord: 5..519
NoneNo IPR availablePANTHERPTHR10196:SF91SUBFAMILY NOT NAMEDcoord: 5..519
IPR018485Carbohydrate kinase, FGGY, C-terminalPFAMPF02782FGGY_Ccoord: 271..470
e-value: 3.5E-59
score: 199.9
IPR018484Carbohydrate kinase, FGGY, N-terminalPFAMPF00370FGGY_Ncoord: 10..263
e-value: 2.0E-70
score: 237.2
IPR000577Carbohydrate kinase, FGGYPIRSFPIRSF000538GlpKcoord: 7..522
e-value: 4.4E-149
score: 494.9
IPR005999Glycerol kinaseTIGRFAMTIGR01311TIGR01311coord: 8..516
e-value: 4.4E-218
score: 722.5
IPR018483Carbohydrate kinase, FGGY, conserved sitePROSITEPS00933FGGY_KINASES_1coord: 144..156
IPR043129ATPase, nucleotide binding domainSUPERFAMILY53067Actin-like ATPase domaincoord: 266..516
IPR043129ATPase, nucleotide binding domainSUPERFAMILY53067Actin-like ATPase domaincoord: 9..266

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg014781.1Spg014781.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046167 glycerol-3-phosphate biosynthetic process
biological_process GO:0019563 glycerol catabolic process
biological_process GO:0016310 phosphorylation
biological_process GO:0006641 triglyceride metabolic process
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0006072 glycerol-3-phosphate metabolic process
cellular_component GO:0005739 mitochondrion
molecular_function GO:0005524 ATP binding
molecular_function GO:0004370 glycerol kinase activity
molecular_function GO:0016773 phosphotransferase activity, alcohol group as acceptor