Spg014656 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg014656
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionCarotenoid cleavage dioxygenase
Locationscaffold3: 46284422 .. 46293106 (+)
RNA-Seq ExpressionSpg014656
SyntenySpg014656
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGAAAAAAATGATGCTCATAATTGGAATTGGAAATCCAAATGATACAAATTTGTCCATGAATTACCGAGAGATTGTATGGTAGTGGAAAGAATTTCCATGGAAATGAACATCCGCATCGTTACGTGGCCAGACTTTCACCCACATGCCTCCACTCTCCATTGATTATTTCTAGACGAAACCAATTTTCTCTGTTGCCAATACAGTCCACTCGCGATCCTGTGACTGATACTGAGCGTGTGATTTGTAGAACTGAAGATGGCGGAGGAGAAGCAGAAGCTCAATGGCGGGGTTGGTGGACGGTCGTTGGTGGAGGTGAATCCGAAGCCGAGCAAAGGGCTGGCATCGAAGGCGACTGATTTGTTGGAGAAGCTCTTTGTGAAGCTTATGTATGATTCTTCAAAGCCTCAGCATTATCTTTCCGGTAATTTCGCTCCGGTTCGCGAAGAGACGCCTCCGATTACCGATCTCCCTGTTAAAGGATATTTGCCGGTGAGTTGGATATCTGTTAGATGATTGTACTCTGTTTTCTGATTTGGAATCTGTTAATTCAGAAGTGTTGATCGATGAATTTAATCGGGTTCGTGTTTGTGGGTGTCTCTCGATGGGCTTTACAATTAGCTTTTAGTATAGTATTCTGCATTCGCATTTGCCGCTTTAAAGATTCTTCCCGCCAAGAACATTCTATGCTATATATTTTTTTTTTTTTTTTAACAATATGTAGGGAGGAGGATTCGAACCACAGTGGTTATCAATACAACTTTATGCCAAACTCTTGTTGGCAAGAACATTCTATGCTACTAAGAGTGAGGAAACCTGTACTGATCGGTTTTCGTATGTTATAACGCTGTCGATTCTTTAGACTGATTTGTTGTTAAGATGGGTACTGCTTCAGTTTTAGAATAAATTGTACCAATATTGAAAATCGAATAGTTATGATAGGCCTTAATACAGTCCGAAACCAGATGTGCGATGCTTCATCAGTTGAGAGAGAGGCTTCATCTGAAAATGTGAATATAAGTCTTGGAGGTCTCGTTTCCTGTATTAACTCAATCCAATGAAACTATCTGAATTGGTGCGACCGCATGTCAAGGAGGGTACGATGTAAAGTTGGTTAGATAATTTGAAAACAATGAAGTGACAACCTAGAGAGAAATTACTAATTCTCTTAATATGGAGTTCGAAGAATTGATGCTTTGAGCTGCAGGATAGTGTAAGAGGAGGCTTTTGAACTGGATTTTTGTCAATTTGTACGTGTGTGTGTTAGATTTAATTCTTTGTTTATGAGCTGTCGACACAACTACATACTGCGTGTTAATGTTATTCTGGTCTATAATTGGGTTGTAATTTTTCCTGATTTCGCTGCCTCTAATGTGGAATGTATTATAACTCAGGAATGCTTAAATGGAGAATTTGTTAGGGTGGGACCAAATCCGAAATTTAGCCCAGTTGCGGGTTATCACTGGTATGGTTTGTTAAATCTTAAAGCAATTTTGTGCACAAGAGTCCTGCATAATCTTAGTAGAGATAAATTATAACTCTCTATCCTACTATCTATTCATGTTGCTTGGTTAGATGAAATGTTCCTTATTTATCCTTTTTTCCATGTGCAGGTTTGATGGAGATGGGTACGTCGCCTTCTACTTCCCAATTCATCCCATTATTTTTGTTTTTTACAATATGTGGTGTGGGAGATTCGAACCACAGATCTCGTGGTCATCAGTATAACTTTATGCCAGTTGAGCTATGCTCTTCTTGGCAATTTATCCCATTATTTCACTTGCAGCTTACTTAAAGATTACATTCGCTTATGTTTTTGGATCTTGATTAATTTCCACAAGTGAATGCCGTTACGAATAAATGTGATGGATTCAGTAATCAATTTGAATTATGAATTGAATAGCTCAATTTTTGTCCCACGGTATGAAATTATCCTTTCGTTGACTTGTTATTTCCCTGTGGAATAGTTATTCAGGATAACTTTATTCATAGAATTTTGAAGGGTTTTCGGAGTACCATTTTAAAACTCTGTGAACTGTAACTGACAATGTCCTCTTGATGCTTGAACATTTAGGCCTTTTGGTGGTATGACTCATAATGAGTAAAAAAAATTATTTTTTGTATTCCCAAAGTCTTATCCGTATAATATTTTCATTAGTTTGTTATCATCTGGGAACTGTAAAATCAGGGTAGTTCTGTCAGAACTTTAAATCTCCTTCAAGAAATATCCAAGTCTTAAAGAGCGTAGTTCTGTTTGACCATTTAATTTTTCCTTCTGTTTTATATATTGCCTGGCTTTTATAGATTATTGATAATTTTCTTTTGCAGCATGATCCATGGATTGCGCATTAAAGATGGAAAAGCAACATATGTTTCCCGTTATGTGAAGACATCACGACTGAAACAAGAAGAACATTTTGGAGGTGCTAAATTCATGAAGGTATTATATAGCTTGCTACTTAATACTTCCATTCTTCTAAATAGAAGTTATTTTGTTCTCCTAGTATGTGGACGTGGGAATTTATTTATTATTATTATTTTTTTTTAATTCAACAACATGTGGGGATTCAATTTTGTGATTGTTTATTCACTCACTAATGTGATATTATGCCGTCTTAATTTATGCATAACTGTGTTCTTCTTGCCATAATGATACTTCTGATATGAATGCAAGGCCTCCAATTCGATTTGTATTCTAGGAGAAAGGCAGCTGTCTACCAAGTAGAATATTTATATTGTTAGTGAGGGGAATGTTTTATGCATGTAAATGTGAATCCCATTTTGTAAGTAATAAAGAAAATAGTAGTCTATTATGGTGAAGATAGGGAATTTTTCGTAAGCAATGAAGGTGTAAAGGGTATTCAGTTGGTAGAGTTTTGGCCTAGAAAGATCTTTGAGAGGGGAGAGTTCAGGCCTCTTGAAGATTTTTTATTCAATGGATGTTACTAGAATTGCTCAAATTATTAGATTGTTAACAATGAAGTCTACATCATATCTGTTTGTATATTGACCTATTCGTTTCTTTACTGTTATTGGTTTCTTTGTTTTACCTCATGCTACAATCTAGAAAATTGTGCTAAATGCTAATTAGTTTCAGGAATGATGAATCAAAAAGTTATTGATTGCTGTTAACTTTTGATGTCATTTCCAGATTGGTGATCTGAAAGGGTTCTTTGGGTTAATAATGGTTAACATGCAAATGCTGAGAGCAAAGTTAAAAGTGTTGGATGTTTCATATGGAACTGGAACAAGTACGATTTTTTTTATTTATTTTTTTTTTAAATTTTTTGTTATTCATAGATGTCCTATGAAATTCTTCCAAATATTATCTTCCTTCAATGTTGCATTTTCTTAGTTTTCTTCAACAACTTTATGTCATTGTTGGTCTTTCATTTTGCTTTGTATTGATGATAGTTAGTCTCTGTTAATCCTTGATCATTTGGGTGACTCTACATGGTTAGGTTTTGGAATGCCACCTGTGTTAAATTTGTACATGATTGTTATCATATTTGACGTTGCCAAGTCCTCAATGTAATGGAGATATACTGGAGCGAGGTATCCTGAAAGGCTCAAGTTTAGTCCCGTTCATTTGTTAACACCACTGATAATAATGTAGGCGTCTCTGTTATAGATATGATGCAAATATGCATACACTGTTATTACTGCTTGTCTTTGTATGGAACTTAAATTTTAATTGTCTGAAGCTGATCATCAATAACTTTTCCAGGTAACACAGCTCTTATATATCACCATGGGAAGCTGCTAGCACTTTCAGAGGCAGATAAACCTTGTAAGTTTACCTTCTCTCTTTCAAGTTCAGATATCTTCTCCTCAAAGATCAGTCTTTAGATCTTGATGGGGAAGGAAAAATACTTCAACTGTTCTCCCCTCCATCCCACTGAATCACAGAATTAGAAGGGAAAAAAATCTTTGAGTATATGGTTTTACTTAATGTAGATGTTATAAAGGTGTTGGAGGATGGAGACCTGCAGACACTTGGTCTGCTGGATTATGACAAGAGATTAACGCACTCCTTCACTGCTCATCCAAAGGTTGACCCAGTCACTGGTATAAGCATCCTAAAAGACACCTGTTAAGTTTATTGAAATATGATGAACTGCTCTGTGCTGATCAATTTCCTGCATGCAGGCGAAATGTTTACATTTGGTTATTCCCATTCACCACCATATGTTACATACAGAGTTATTTCCAAGGATGGTCTCATGCATGACCCTGTACCAATCACAATTCCAAACCCAATCATGATGCATGACTTCGCCATTACTGAAAATTATGCAATTTTTATGGATCTTCCTTTATATTTTAAACCCAAGGTATGACATTTATTTAACAATTTTTGTTATTAATTTTATATTGTCAATCCAGATAACTCATGCTATTGAATAACAAAATAATCCATTGTATTTAAACAAGGAAGGACATAACTAGTGGCATAAATTAGTGAACCCGGCCTCATCTCCCAAACTCAAATTTAGGAAATTGGTGGCCTTCCCCTTCTCTTTTCCATTTGTAAATTGCTACGCGTGTTCTTATCTTAGGATTTAAATGGCTTGTGTGGACCATCTGATGCTACCAACATTCTCATAATTTGCTTTTACGAATTCAACCAGCATTCCATTTCCTGCCAATGGTATAAGAAAGCAACAGTAATGTACGCAACATAGAGAAGTACATTTTCCATTAATGATCTAGGCATCAATTTATACCTAAAAAGGTATTGCCTCTCAAATTCAGAAATAGTTAAAAGACTACATAAAGAAAAGGAATTAAAGTTATCCTGGAAAATCTCACTAAAGAACCAAATGTGATTAAACAGAAACGACACCTTTAATCCAATATGTGCCTGGGAGAATTTCGAGTGTATTTCAATTCACCTTCATTCTTATGAAGGAACTTCCACACTTATTACCGGTTTAATTTTCCCTTCAGTTGACTGACTCTGACTGTACATACATGTGTTGTTTTATTGGATTGTAGTTCAATTATTAGCAACAGGATGGTTAAATTCTGAACCTTTTAATCTTATGGTTGGTCGTGTAATAATTAAAAACTTGTTAATTTTCAGGAAATGGTGAAAGAAAATAAATTAATTTTCACATTTGATGCTACAAAAAGAGCTCGGTTTGGTGTGCTTCCGCGATATGCAAGAGATGACTTGCTTATGCGATGGTTTGAGCTTCCAAATTGTTTTATATTTCATAATGGTGAGTACTTGTTCTACCCATGTTTTTGAAAGCAAGAAATATCTCTGGCATGCCGTTCCATTTGTGTTATGAATTGCTAAATTACAATCTTAAATAATGATTTCATTATTGGTACTACAACGGTGGCAATGGAAAAAATTCATGTGATTGGGAAATTGGAGATTCTATCTTTAGTTCTATCTTTATAGAATTTCCTCGGATGGTTCCTCTTTGGCTCATTTTCTGTTGGACTTTACTAGTTTTTTTTTTATATTTATGTTGTGTGTAGGGATGTTTCTACATGATTATAGTTGATTTGATGCAAGATTTTATGTTGATATTTGATTTATCTCTTCATTTATGCCCTGATTTTTTAAGGGTTTTACCTTCGTTTTTTTCCTCAAGAACCTTCCAGTCCTTATTGCAATCTGTTTATTTTGCTGATAAAACAGAATGTATTATTGTCCTTACTCTTTCCATTTTCATCATTTATGTTGTTTACTTCTGCTTAGCTAATGCCTGGGAGGAAGGAGATGAAGTAGTTTTGATTACTTGCCGTCTTGAGAATCCAGACTTGGACATGGTCAGTGGGTCTGTCAAAGAGAAGCTTGAGAACTTCGCAAATGAGCTGTAGGACTCTAATCCATTTCTACATATGAAAGAAAAGTTCTTGTTGTAGCTGTGATATTTCTAATTATTTTTCCATCTTTCAGGTATGAGATGAGATTCAATCTTAAATCTGGTTTAGCTTCACAGAAGAAACTCTCGGAATCTGCTGTAGATTTTCCTAGAGTGAATGAATGGTACACTGGCAGGTATTTTAATTCCCTTATTTTTCTAGTATTTGGAAAAGAATATCTGCTTAAATCCATGGGGACATATGAAGAGACATCCAACTTTGGAGTAAGAAACACGTGAATTAAAGGACATTTCTTTCATTTGCAATTTTCCAATTAAAGATCATTAACCCTGTTTAGAAGACATCTTCAGAAACACTATTAACCATAGGGGAATTTAGGTTTTATTGTTTGTATTGGAATACCATACATGCAAAAATTACGTAAAGGTAGAGCTTTTATTTAAAGCAGATAGCAGGTTTACAATGTTTTAGCGATAGCACTTGTATGTCATCTATTGCAGTTGTAAGAGATTCGGATGATGTTGGTCATCATTTCCTTGGTCAAGATAATTGGGTTTTGAACAAGAAACAGAGTGTAATGAAGGTTTTAAAAGCAGAGAGATATTAGCTTGAAGGTCAGTCAAATGACAGTAAATAACAAAGTAAAACAAGTAAACTGAAGCAGTGCTGATAATAAATGTTAGCCTGTTGACCACACTTAAGATTGATTCGTAACTACAGTCCCTTCTTTCTATTGATCTATATAATTCTCACGTTCCATTTTGTCCTGGCTTTGATGAAATGGATTCCAACTTATAGAATTGGTGAATGAGTACACGTGCTCTGTATGCAATCAGGAAGCAACGATACGTATTTGGAACTACACTGGACAGCATTGCAAAAGTCACGGGGATTGCTAAATTTGATCTGCATGCTAAACCTGAAACTGGAAAAACAAAGATTGAAGTTGGAGGAAATGTTCAGGGCCTCTATGACCTTGGACCTGGTAGATTTGGTTCTGAAGCTATCTTTGTTCCTCGCGTACCTGGCACCACTTCAGAAGAAGATGATGGCTACCTAATATTATTCGTACATGATGAGAACACTGGGTACTGCTTTCCACTTCTAATATATTGTTAATTTTGTGTCATGTATTTATATATTTATATACATTATATGAGTAGTAGCTGGAATACGGATGCCTTTCTTAGGCATGCAAATTGGATATCCTATTACTAAGTGGCTGTTATCAGTTAGAACGTCTTGCTGTTTAGAAAGTTAAAGGTCCACAGTTGGTGGGAGTTTTATAATTTTCTAAATTATTTTTTTTCTTATAGCTTTTGCAATTCATGAATATAGTCCATGATCCAAAATACCAACTCGCCTCTCAATAGAATTTAACTTCAACCCTGTAGCAGCAGCCTTGATATTTGCATAAGAACGCAAGTAGGCAGTTTTACTGAATACTTGTGATGTATCAAATTCAGTTTATTTTACTAAAGACGGAGGATAATAATTTTCTTTGTTTCCTGCAGAAAATCAGTAGTGAATGTCATTGATGCAAAAACTATGTCATCTGAGCCTGTTGCAGTTGTTGAATTGCCACACAGAGTTCCATATGGGTTCCATGCCTTCTTTGTAACAGAGGTTAGTGTCAAAGTCATTTGCATTCAGTTGGCTTTCTGATAAACTTCGGGTCCGTTTGGAATACATTTTAATTTAAATGTTTTTCAAGAATGCATTTTTTAAAAAACATTTTGTATAAAAATTGTTTAGAATTTAAACACTCATATGTTTGGTTGCATTTCCTCATAGATGTTTTTATACTCAAGTTATTGTATACTTAAAAGACTATTTTTAAAAAAGTTGATTTCAAGTGTTTTTCTCAAAATGATTTATTTATCAATCTCATTTTCTAACAAGTTATTTTTAGTAGTTGTCAAACACTACAAATTTTTTTAAAATTAAACACTTGAAAAGTTAAACCAAACACACCCTTTGACAAAATAATCAATCATAAGTGTCTTTCGAATTCTCCAGTCAGCTTCACCTAAATATATAAACCATTGTTGTCCAATATTGGCAATCGTGGACCATGTGTGGCATGTGAATTATATTATCACAGACTCAAGAATTATCATATGTTTAGACTATCGCCTGACAACGAGTTATTTCTAGCTACCATACACTTGAGTTTAGGGCAATATTGGCAATTATAGTCTCTTGCCTTTCACGAGTTATCCAATATTCAGCCTGAGTGTGTGAATTACCTTAGAAGTAGAAGTATTCTAGCCGTTAGCTTTTGACATTCCCTTATTTCAGTTTTTTGGCCTTTTACATTCGACATTCATTGTTTCTGAACAGGAGCAACTTCAAGAACAAGAAAGGTTTTGACTGTACAGTTATTGGAAACTTAATGACAGAATGTGACAGAGGACTTGAAGAAGACAAACAAACAATTGTTAGCATGATATCTATTCATCTTTTATCTTGAAATATTTGTACCTCTCCTTGTTTCTGATGATTAATATTATAACATTATGTGCTTCCTCCCTCCAAGATCTTGAAGTGAAGACTTGCATTTATGAATAAAATTACCCTTCTCATTGTCAAGAATCATGGTCAAAGAATCTGAAACTTTCTCGAACAGTGAAAAATGAATCACTTTAGAAGTGTATATTCTATTGTTCACTTCAATGCCCACCACCTTCTGTTAATGCTAGTCCCTCCATTTCTGTTTTAACAGTATGATTTTGGTCTATTGTTCACTTATGCATTAGCCTCGTTAGAGGCTCATATGCAAGCCTTAATGAACTTGTTTGCTGAGGAGTTTTTTGAAATATACACACGAATGGATGAATGA

mRNA sequence

ATGGCGGAGGAGAAGCAGAAGCTCAATGGCGGGGTTGGTGGACGGTCGTTGGTGGAGGTGAATCCGAAGCCGAGCAAAGGGCTGGCATCGAAGGCGACTGATTTGTTGGAGAAGCTCTTTGTGAAGCTTATGTATGATTCTTCAAAGCCTCAGCATTATCTTTCCGGTAATTTCGCTCCGGTTCGCGAAGAGACGCCTCCGATTACCGATCTCCCTGTTAAAGGATATTTGCCGGAATGCTTAAATGGAGAATTTGTTAGGGTGGGACCAAATCCGAAATTTAGCCCAGTTGCGGGTTATCACTGGTTTGATGGAGATGGCATGATCCATGGATTGCGCATTAAAGATGGAAAAGCAACATATGTTTCCCGTTATGTGAAGACATCACGACTGAAACAAGAAGAACATTTTGGAGGTGCTAAATTCATGAAGATTGGTGATCTGAAAGGGTTCTTTGGGTTAATAATGGTTAACATGCAAATGCTGAGAGCAAAGTTAAAAGTGTTGGATGTTTCATATGGAACTGGAACAAGTAACACAGCTCTTATATATCACCATGGGAAGCTGCTAGCACTTTCAGAGGCAGATAAACCTTATGTTATAAAGGTGTTGGAGGATGGAGACCTGCAGACACTTGGTCTGCTGGATTATGACAAGAGATTAACGCACTCCTTCACTGCTCATCCAAAGGTTGACCCAGTCACTGGCGAAATGTTTACATTTGGTTATTCCCATTCACCACCATATGTTACATACAGAGTTATTTCCAAGGATGGTCTCATGCATGACCCTGTACCAATCACAATTCCAAACCCAATCATGATGCATGACTTCGCCATTACTGAAAATTATGCAATTTTTATGGATCTTCCTTTATATTTTAAACCCAAGGAAATGGTGAAAGAAAATAAATTAATTTTCACATTTGATGCTACAAAAAGAGCTCGGTTTGGTGTGCTTCCGCGATATGCAAGAGATGACTTGCTTATGCGATGGTTTGAGCTTCCAAATTGTTTTATATTTCATAATGCTAATGCCTGGGAGGAAGGAGATGAAGTAGTTTTGATTACTTGCCGTCTTGAGAATCCAGACTTGGACATGGTCAGTGGGTCTGTCAAAGAGAAGCTTGAGAACTTCGCAAATGAGCTGTATGAGATGAGATTCAATCTTAAATCTGGTTTAGCTTCACAGAAGAAACTCTCGGAATCTGCTGTAGATTTTCCTAGAGTGAATGAATGGTACACTGGCAGGTATTTTAATTCCCTTATTTTTCTAGTATTTGGAAAAGAATATCTGCTTAAATCCATGGGGACATATGAAGAGACATCCAACTTTGGAGTAGAGCTTTTATTTAAAGCAGATAGCAGAAAATCAGTAGTGAATGTCATTGATGCAAAAACTATGTCATCTGAGCCTGTTGCAGTTGTTGAATTGCCACACAGAGTTCCATATGGGTTCCATGCCTTCTTTGTAACAGAGTCTCTTGCCTTTCACGAGTTATCCAATATTCAGCCTGAGTGTGTGAATTACCTTAGAAGTAGAAGTATTCTAGCCTATGATTTTGGTCTATTGTTCACTTATGCATTAGCCTCGTTAGAGGCTCATATGCAAGCCTTAATGAACTTGTTTGCTGAGGAGTTTTTTGAAATATACACACGAATGGATGAATGA

Coding sequence (CDS)

ATGGCGGAGGAGAAGCAGAAGCTCAATGGCGGGGTTGGTGGACGGTCGTTGGTGGAGGTGAATCCGAAGCCGAGCAAAGGGCTGGCATCGAAGGCGACTGATTTGTTGGAGAAGCTCTTTGTGAAGCTTATGTATGATTCTTCAAAGCCTCAGCATTATCTTTCCGGTAATTTCGCTCCGGTTCGCGAAGAGACGCCTCCGATTACCGATCTCCCTGTTAAAGGATATTTGCCGGAATGCTTAAATGGAGAATTTGTTAGGGTGGGACCAAATCCGAAATTTAGCCCAGTTGCGGGTTATCACTGGTTTGATGGAGATGGCATGATCCATGGATTGCGCATTAAAGATGGAAAAGCAACATATGTTTCCCGTTATGTGAAGACATCACGACTGAAACAAGAAGAACATTTTGGAGGTGCTAAATTCATGAAGATTGGTGATCTGAAAGGGTTCTTTGGGTTAATAATGGTTAACATGCAAATGCTGAGAGCAAAGTTAAAAGTGTTGGATGTTTCATATGGAACTGGAACAAGTAACACAGCTCTTATATATCACCATGGGAAGCTGCTAGCACTTTCAGAGGCAGATAAACCTTATGTTATAAAGGTGTTGGAGGATGGAGACCTGCAGACACTTGGTCTGCTGGATTATGACAAGAGATTAACGCACTCCTTCACTGCTCATCCAAAGGTTGACCCAGTCACTGGCGAAATGTTTACATTTGGTTATTCCCATTCACCACCATATGTTACATACAGAGTTATTTCCAAGGATGGTCTCATGCATGACCCTGTACCAATCACAATTCCAAACCCAATCATGATGCATGACTTCGCCATTACTGAAAATTATGCAATTTTTATGGATCTTCCTTTATATTTTAAACCCAAGGAAATGGTGAAAGAAAATAAATTAATTTTCACATTTGATGCTACAAAAAGAGCTCGGTTTGGTGTGCTTCCGCGATATGCAAGAGATGACTTGCTTATGCGATGGTTTGAGCTTCCAAATTGTTTTATATTTCATAATGCTAATGCCTGGGAGGAAGGAGATGAAGTAGTTTTGATTACTTGCCGTCTTGAGAATCCAGACTTGGACATGGTCAGTGGGTCTGTCAAAGAGAAGCTTGAGAACTTCGCAAATGAGCTGTATGAGATGAGATTCAATCTTAAATCTGGTTTAGCTTCACAGAAGAAACTCTCGGAATCTGCTGTAGATTTTCCTAGAGTGAATGAATGGTACACTGGCAGGTATTTTAATTCCCTTATTTTTCTAGTATTTGGAAAAGAATATCTGCTTAAATCCATGGGGACATATGAAGAGACATCCAACTTTGGAGTAGAGCTTTTATTTAAAGCAGATAGCAGAAAATCAGTAGTGAATGTCATTGATGCAAAAACTATGTCATCTGAGCCTGTTGCAGTTGTTGAATTGCCACACAGAGTTCCATATGGGTTCCATGCCTTCTTTGTAACAGAGTCTCTTGCCTTTCACGAGTTATCCAATATTCAGCCTGAGTGTGTGAATTACCTTAGAAGTAGAAGTATTCTAGCCTATGATTTTGGTCTATTGTTCACTTATGCATTAGCCTCGTTAGAGGCTCATATGCAAGCCTTAATGAACTTGTTTGCTGAGGAGTTTTTTGAAATATACACACGAATGGATGAATGA

Protein sequence

MAEEKQKLNGGVGGRSLVEVNPKPSKGLASKATDLLEKLFVKLMYDSSKPQHYLSGNFAPVREETPPITDLPVKGYLPECLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKATYVSRYVKTSRLKQEEHFGGAKFMKIGDLKGFFGLIMVNMQMLRAKLKVLDVSYGTGTSNTALIYHHGKLLALSEADKPYVIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPVTGEMFTFGYSHSPPYVTYRVISKDGLMHDPVPITIPNPIMMHDFAITENYAIFMDLPLYFKPKEMVKENKLIFTFDATKRARFGVLPRYARDDLLMRWFELPNCFIFHNANAWEEGDEVVLITCRLENPDLDMVSGSVKEKLENFANELYEMRFNLKSGLASQKKLSESAVDFPRVNEWYTGRYFNSLIFLVFGKEYLLKSMGTYEETSNFGVELLFKADSRKSVVNVIDAKTMSSEPVAVVELPHRVPYGFHAFFVTESLAFHELSNIQPECVNYLRSRSILAYDFGLLFTYALASLEAHMQALMNLFAEEFFEIYTRMDE
Homology
BLAST of Spg014656 vs. NCBI nr
Match: XP_022148582.1 (carotenoid 9,10(9',10')-cleavage dioxygenase 1 [Momordica charantia])

HSP 1 Score: 886.3 bits (2289), Expect = 1.3e-253
Identity = 445/538 (82.71%), Postives = 464/538 (86.25%), Query Frame = 0

Query: 1   MAEEKQKLNGGVGGRSLVEVNPKPSKGLASKATDLLEKLFVKLMYDSSKPQHYLSGNFAP 60
           MAEEKQKLNGGV  RSLVEVNPKPSKGLASKATDLLEKLFVKLMYD+S PQHYLSGNFAP
Sbjct: 1   MAEEKQKLNGGVVDRSLVEVNPKPSKGLASKATDLLEKLFVKLMYDASNPQHYLSGNFAP 60

Query: 61  VREETPPITDLPVKGYLPECLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKAT 120
           VR+ETPPITDLPVKGYLPECLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKAT
Sbjct: 61  VRDETPPITDLPVKGYLPECLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKAT 120

Query: 121 YVSRYVKTSRLKQEEHFGGAKFMKIGDLKGFFGLIMVNMQMLRAKLKVLDVSYGTGTSNT 180
           YVSRYVKTSRLKQEE+FGGAKFMKIGDLKGFFGLIMVNMQMLRAKLKVLDVSYGTGT NT
Sbjct: 121 YVSRYVKTSRLKQEEYFGGAKFMKIGDLKGFFGLIMVNMQMLRAKLKVLDVSYGTGTGNT 180

Query: 181 ALIYHHGKLLALSEADKPYVIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPVTGEMFT 240
           ALIYHHGKLLALSEADKPYVIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPVTGEMFT
Sbjct: 181 ALIYHHGKLLALSEADKPYVIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPVTGEMFT 240

Query: 241 FGYSHSPPYVTYRVISKDGLMHDPVPITIPNPIMMHDFAITENYAIFMDLPLYFKPKEMV 300
           FGYSHSPPYVTYRVISKDGLMHDP+PITIPNP+MMHDFAITENYAIFMDLPLYFKPKEMV
Sbjct: 241 FGYSHSPPYVTYRVISKDGLMHDPIPITIPNPVMMHDFAITENYAIFMDLPLYFKPKEMV 300

Query: 301 KENKLIFTFDATKRARFGVLPRYARDDLLMRWFELPNCFIFHNANAWEEGDEVVLITCRL 360
           KENKLIFTFDATKRARFGVLPRYARDDLL+RWFELPNCFIFHNANAWEEGDEVVLITCRL
Sbjct: 301 KENKLIFTFDATKRARFGVLPRYARDDLLIRWFELPNCFIFHNANAWEEGDEVVLITCRL 360

Query: 361 ENPDLDMVSGSVKEKLENFANELYEMRFNLKSGLASQKKLSESAVDFPRVNEWYTGR--- 420
           ENPDLDMVSGSVKEKLENF+NELYEMRFNLKSG ASQKKLSESAVDFPRVNE YTGR   
Sbjct: 361 ENPDLDMVSGSVKEKLENFSNELYEMRFNLKSGRASQKKLSESAVDFPRVNESYTGRKQQ 420

Query: 421 -YFNSLIFLV--------------------------------------FGKEYLLKSM-- 480
             + +++  +                                      FG E +      
Sbjct: 421 YVYGTILDSIAKVTGIAKFDLNAKPETGKTKIEVGGNVQGLYDLGPGRFGSEAIFVPRIP 480

Query: 481 -GTYEETSNFGVELLFKADSRKSVVNVIDAKTMSSEPVAVVELPHRVPYGFHAFFVTE 494
             T EE   + +  +   ++ KS VNVIDAKTMS+EPVAVVELPHRVPYGFHAFFVTE
Sbjct: 481 GTTSEEDDGYLIFFVHDENTGKSSVNVIDAKTMSTEPVAVVELPHRVPYGFHAFFVTE 538

BLAST of Spg014656 vs. NCBI nr
Match: AFU91489.1 (carotenoid cleavage dioxygenase 1 [Momordica charantia])

HSP 1 Score: 885.2 bits (2286), Expect = 3.0e-253
Identity = 444/538 (82.53%), Postives = 464/538 (86.25%), Query Frame = 0

Query: 1   MAEEKQKLNGGVGGRSLVEVNPKPSKGLASKATDLLEKLFVKLMYDSSKPQHYLSGNFAP 60
           MAEEKQKLNGGV  RSLVEVNPKPSKGLASKATDLLEKLFVKLMYD+S PQHYLSGNFAP
Sbjct: 1   MAEEKQKLNGGVVDRSLVEVNPKPSKGLASKATDLLEKLFVKLMYDASNPQHYLSGNFAP 60

Query: 61  VREETPPITDLPVKGYLPECLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKAT 120
           VR+ETPPITDLPVKGYLPECLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKAT
Sbjct: 61  VRDETPPITDLPVKGYLPECLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKAT 120

Query: 121 YVSRYVKTSRLKQEEHFGGAKFMKIGDLKGFFGLIMVNMQMLRAKLKVLDVSYGTGTSNT 180
           YVSRYVKTSRLKQEE+FGGAKFMKIGDLKGFFGLIMVNMQMLRAKLKVLDVSYGTGT NT
Sbjct: 121 YVSRYVKTSRLKQEEYFGGAKFMKIGDLKGFFGLIMVNMQMLRAKLKVLDVSYGTGTGNT 180

Query: 181 ALIYHHGKLLALSEADKPYVIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPVTGEMFT 240
           ALIYHHGKLLALSEADKPYVIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPVTGEMFT
Sbjct: 181 ALIYHHGKLLALSEADKPYVIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPVTGEMFT 240

Query: 241 FGYSHSPPYVTYRVISKDGLMHDPVPITIPNPIMMHDFAITENYAIFMDLPLYFKPKEMV 300
           FGYSHSPPYVTYRVISKDGLMHDP+PITIPNP+MMHDFAITENYAIFMDLPLYFKPKE+V
Sbjct: 241 FGYSHSPPYVTYRVISKDGLMHDPIPITIPNPVMMHDFAITENYAIFMDLPLYFKPKELV 300

Query: 301 KENKLIFTFDATKRARFGVLPRYARDDLLMRWFELPNCFIFHNANAWEEGDEVVLITCRL 360
           KENKLIFTFDATKRARFGVLPRYARDDLL+RWFELPNCFIFHNANAWEEGDEVVLITCRL
Sbjct: 301 KENKLIFTFDATKRARFGVLPRYARDDLLIRWFELPNCFIFHNANAWEEGDEVVLITCRL 360

Query: 361 ENPDLDMVSGSVKEKLENFANELYEMRFNLKSGLASQKKLSESAVDFPRVNEWYTGR--- 420
           ENPDLDMVSGSVKEKLENF+NELYEMRFNLKSG ASQKKLSESAVDFPRVNE YTGR   
Sbjct: 361 ENPDLDMVSGSVKEKLENFSNELYEMRFNLKSGRASQKKLSESAVDFPRVNESYTGRKQQ 420

Query: 421 -YFNSLIFLV--------------------------------------FGKEYLLKSM-- 480
             + +++  +                                      FG E +      
Sbjct: 421 YVYGTILDSIAKVTGIAKFDLNAKPETGKTKIEVGGNVQGLYDPGPGRFGSEAIFVPRIP 480

Query: 481 -GTYEETSNFGVELLFKADSRKSVVNVIDAKTMSSEPVAVVELPHRVPYGFHAFFVTE 494
             T EE   + +  +   ++ KS VNVIDAKTMS+EPVAVVELPHRVPYGFHAFFVTE
Sbjct: 481 GTTSEEDDGYLIFFVHDENTGKSSVNVIDAKTMSTEPVAVVELPHRVPYGFHAFFVTE 538

BLAST of Spg014656 vs. NCBI nr
Match: KAG6575691.1 (Carotenoid 9,10(9',10')-cleavage dioxygenase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 882.5 bits (2279), Expect = 1.9e-252
Identity = 445/539 (82.56%), Postives = 466/539 (86.46%), Query Frame = 0

Query: 1   MAEEKQKLNGGVGGRSLVEVNPKPSKGLASKATDLLEKLFVKLMYDSSKPQHYLSGNFAP 60
           MAEEKQ+LNGGV G SLVEVNPKPSKGLASK TDLLEKLFVKLMYDSSKPQHYLSGNFAP
Sbjct: 1   MAEEKQRLNGGVVGGSLVEVNPKPSKGLASKVTDLLEKLFVKLMYDSSKPQHYLSGNFAP 60

Query: 61  VREETPPITDLPVKGYLPECLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKAT 120
           V +ETPPITDLPVKGYLPECLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKAT
Sbjct: 61  VGDETPPITDLPVKGYLPECLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKAT 120

Query: 121 YVSRYVKTSRLKQEEHFGGAKFMKIGDLKGFFGLIMVNMQMLRAKLKVLDVSYGTGTSNT 180
           YVSRYV+TSRLKQEE+FGGAKFMKIGDLKGFFGL+MVNMQ+LRAKLKVLDVSYGTGT NT
Sbjct: 121 YVSRYVRTSRLKQEEYFGGAKFMKIGDLKGFFGLVMVNMQILRAKLKVLDVSYGTGTGNT 180

Query: 181 ALIYHHGKLLALSEADKPYVIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPVTGEMFT 240
           ALIYHHGKLLALSEADKPYVIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPVTGEMFT
Sbjct: 181 ALIYHHGKLLALSEADKPYVIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPVTGEMFT 240

Query: 241 FGYSHSPPYVTYRVISKDGLMHDPVPITIPNPIMMHDFAITENYAIFMDLPLYFKPKEMV 300
           FGYSHSPPYVTYRVISKDGLMHDPVPITIPNP+MMHDFAITENYAIFMDLPLYFKPKEMV
Sbjct: 241 FGYSHSPPYVTYRVISKDGLMHDPVPITIPNPVMMHDFAITENYAIFMDLPLYFKPKEMV 300

Query: 301 KENKLIFTFDATKRARFGVLPRYARDDLLMRWFELPNCFIFHNANAWEEGDEVVLITCRL 360
           KENKLIFTFDATK+ARFGVLPRYA+DDLLMRWFELPNCFIFHNANAWEEGDEVVLITCRL
Sbjct: 301 KENKLIFTFDATKKARFGVLPRYAKDDLLMRWFELPNCFIFHNANAWEEGDEVVLITCRL 360

Query: 361 ENPDLDMVSGSVKEKLENFANELYEMRFNLKSGLASQKKLSESAVDFPRVNEWYTGR--- 420
           ENPDLDMVSGSVKEKLENF+NELYEMRFNLKSGL+SQ+KLSESAVDFPRVNE YTGR   
Sbjct: 361 ENPDLDMVSGSVKEKLENFSNELYEMRFNLKSGLSSQRKLSESAVDFPRVNECYTGRKQR 420

Query: 421 --YFNSLIFLV-------------------------------------FGKE--YLLKSM 480
             Y  +L  +                                      FG E  ++ +  
Sbjct: 421 YVYGTTLDSIAKVTGIAKFDLHAKREIGRTKIEVGGNVQGLYDLGPGRFGSEAIFVPRIP 480

Query: 481 GTYEETSNFGVELLFKADSR--KSVVNVIDAKTMSSEPVAVVELPHRVPYGFHAFFVTE 494
           GT  E  + G  +LF  D    KS VNV+DAKTMSSEPVAVVELPHRVPYGFHAFFV+E
Sbjct: 481 GTTSEEDD-GYLILFVHDENTGKSAVNVVDAKTMSSEPVAVVELPHRVPYGFHAFFVSE 538

BLAST of Spg014656 vs. NCBI nr
Match: XP_022953563.1 (carotenoid 9,10(9',10')-cleavage dioxygenase 1 isoform X1 [Cucurbita moschata])

HSP 1 Score: 881.3 bits (2276), Expect = 4.3e-252
Identity = 444/539 (82.37%), Postives = 466/539 (86.46%), Query Frame = 0

Query: 1   MAEEKQKLNGGVGGRSLVEVNPKPSKGLASKATDLLEKLFVKLMYDSSKPQHYLSGNFAP 60
           MAEEKQ+LNGGV G SLVEVNPKPSKGLASK TDLLEKLFVKLMYDSSKPQHYLSGNFAP
Sbjct: 1   MAEEKQRLNGGVVGGSLVEVNPKPSKGLASKVTDLLEKLFVKLMYDSSKPQHYLSGNFAP 60

Query: 61  VREETPPITDLPVKGYLPECLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKAT 120
           V +ETPPITDLPVKGYLPECLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKAT
Sbjct: 61  VGDETPPITDLPVKGYLPECLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKAT 120

Query: 121 YVSRYVKTSRLKQEEHFGGAKFMKIGDLKGFFGLIMVNMQMLRAKLKVLDVSYGTGTSNT 180
           YVSRYV+TSRLKQEE+FGGAKFMKIGDLKGFFGL+MVNMQ+LRAKLKVLDVSYGTGT NT
Sbjct: 121 YVSRYVRTSRLKQEEYFGGAKFMKIGDLKGFFGLVMVNMQILRAKLKVLDVSYGTGTGNT 180

Query: 181 ALIYHHGKLLALSEADKPYVIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPVTGEMFT 240
           ALIYHHGKLLALSEADKPYVIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPVTGEMFT
Sbjct: 181 ALIYHHGKLLALSEADKPYVIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPVTGEMFT 240

Query: 241 FGYSHSPPYVTYRVISKDGLMHDPVPITIPNPIMMHDFAITENYAIFMDLPLYFKPKEMV 300
           FGYSH+PPYVTYRVISKDGLMHDPVPITIPNP+MMHDFAITENYAIFMDLPLYFKPKEMV
Sbjct: 241 FGYSHTPPYVTYRVISKDGLMHDPVPITIPNPVMMHDFAITENYAIFMDLPLYFKPKEMV 300

Query: 301 KENKLIFTFDATKRARFGVLPRYARDDLLMRWFELPNCFIFHNANAWEEGDEVVLITCRL 360
           KENKLIFTFDATK+ARFGVLPRYA+DDLLMRWFELPNCFIFHNANAWEEGDEVVLITCRL
Sbjct: 301 KENKLIFTFDATKKARFGVLPRYAKDDLLMRWFELPNCFIFHNANAWEEGDEVVLITCRL 360

Query: 361 ENPDLDMVSGSVKEKLENFANELYEMRFNLKSGLASQKKLSESAVDFPRVNEWYTGR--- 420
           ENPDLDMVSGSVKEKLENF+NELYEMRFNLKSGL+SQ+KLSESAVDFPRVNE YTGR   
Sbjct: 361 ENPDLDMVSGSVKEKLENFSNELYEMRFNLKSGLSSQRKLSESAVDFPRVNECYTGRKQR 420

Query: 421 --YFNSLIFLV-------------------------------------FGKE--YLLKSM 480
             Y  +L  +                                      FG E  ++ +  
Sbjct: 421 YVYGTTLDSIAKVTGIAKFDLHAKRETGRTKIEVGGNVRGLYDLGPGRFGSEAIFVPRIP 480

Query: 481 GTYEETSNFGVELLFKADSR--KSVVNVIDAKTMSSEPVAVVELPHRVPYGFHAFFVTE 494
           GT  E  + G  +LF  D    KS VNV+DAKTMSSEPVAVVELPHRVPYGFHAFFV+E
Sbjct: 481 GTTSEEDD-GYLILFVHDENTGKSAVNVVDAKTMSSEPVAVVELPHRVPYGFHAFFVSE 538

BLAST of Spg014656 vs. NCBI nr
Match: XP_022991870.1 (carotenoid 9,10(9',10')-cleavage dioxygenase 1 isoform X1 [Cucurbita maxima])

HSP 1 Score: 881.3 bits (2276), Expect = 4.3e-252
Identity = 444/539 (82.37%), Postives = 466/539 (86.46%), Query Frame = 0

Query: 1   MAEEKQKLNGGVGGRSLVEVNPKPSKGLASKATDLLEKLFVKLMYDSSKPQHYLSGNFAP 60
           MAEEKQ+LNGGV G SLVEVNPKPSKGLASK TDLLEKLFVKLMYDSSKPQHYLSGNFAP
Sbjct: 1   MAEEKQRLNGGVVGGSLVEVNPKPSKGLASKVTDLLEKLFVKLMYDSSKPQHYLSGNFAP 60

Query: 61  VREETPPITDLPVKGYLPECLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKAT 120
           V +ETPPITDLPVKGYLPECLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKAT
Sbjct: 61  VGDETPPITDLPVKGYLPECLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKAT 120

Query: 121 YVSRYVKTSRLKQEEHFGGAKFMKIGDLKGFFGLIMVNMQMLRAKLKVLDVSYGTGTSNT 180
           YVSRYV+TSRLKQEE+FGGAKFMKIGDLKGFFGL+MVNMQ+LRAKLKVLDVSYGTGT NT
Sbjct: 121 YVSRYVRTSRLKQEEYFGGAKFMKIGDLKGFFGLVMVNMQILRAKLKVLDVSYGTGTGNT 180

Query: 181 ALIYHHGKLLALSEADKPYVIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPVTGEMFT 240
           ALIYHHGKLLALSEADKPYVIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPVTGEMFT
Sbjct: 181 ALIYHHGKLLALSEADKPYVIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPVTGEMFT 240

Query: 241 FGYSHSPPYVTYRVISKDGLMHDPVPITIPNPIMMHDFAITENYAIFMDLPLYFKPKEMV 300
           FGYSH+PPYVTYRVISKDGLMHDPVPITIPNP+MMHDFAITENYAIFMDLPLYFKPKEMV
Sbjct: 241 FGYSHTPPYVTYRVISKDGLMHDPVPITIPNPVMMHDFAITENYAIFMDLPLYFKPKEMV 300

Query: 301 KENKLIFTFDATKRARFGVLPRYARDDLLMRWFELPNCFIFHNANAWEEGDEVVLITCRL 360
           KENKLIFTFDATK+ARFGVLPRYA+DDLLMRWFELPNCFIFHNANAWEEGDEVVLITCRL
Sbjct: 301 KENKLIFTFDATKKARFGVLPRYAKDDLLMRWFELPNCFIFHNANAWEEGDEVVLITCRL 360

Query: 361 ENPDLDMVSGSVKEKLENFANELYEMRFNLKSGLASQKKLSESAVDFPRVNEWYTGR--- 420
           ENPDLDMVSGSVKEKLENF+NELYEMRFNLKSGL+SQ+KLSESAVDFPRVNE YTGR   
Sbjct: 361 ENPDLDMVSGSVKEKLENFSNELYEMRFNLKSGLSSQRKLSESAVDFPRVNECYTGRKQR 420

Query: 421 --YFNSLIFLV-------------------------------------FGKE--YLLKSM 480
             Y  +L  +                                      FG E  ++ +  
Sbjct: 421 YVYGTTLDSIAKVTGIAKFDLHAKRETGRTKIEVGGNVQGLYDLGPGRFGSEAIFVPRIP 480

Query: 481 GTYEETSNFGVELLFKADSR--KSVVNVIDAKTMSSEPVAVVELPHRVPYGFHAFFVTE 494
           GT  E  + G  +LF  D    KS VNV+DAKTMSSEPVAVVELPHRVPYGFHAFFV+E
Sbjct: 481 GTTSEEDD-GYLILFVHDENTGKSAVNVVDAKTMSSEPVAVVELPHRVPYGFHAFFVSE 538

BLAST of Spg014656 vs. ExPASy Swiss-Prot
Match: O65572 (Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=CCD1 PE=1 SV=2)

HSP 1 Score: 775.4 bits (2001), Expect = 4.3e-223
Identity = 373/522 (71.46%), Postives = 425/522 (81.42%), Query Frame = 0

Query: 14  GRSLVEVNPKPSKGLASKATDLLEKLFVKLMYDSSKPQHYLSGNFAPVREETPPITDLPV 73
           G S++ V+P+PSKG +SK  DLLE+L VKLM+D+S P HYLSGNFAP+R+ETPP+ DLPV
Sbjct: 8   GSSIISVHPRPSKGFSSKLLDLLERLVVKLMHDASLPLHYLSGNFAPIRDETPPVKDLPV 67

Query: 74  KGYLPECLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKATYVSRYVKTSRLKQ 133
            G+LPECLNGEFVRVGPNPKF  VAGYHWFDGDGMIHG+RIKDGKATYVSRYVKTSRLKQ
Sbjct: 68  HGFLPECLNGEFVRVGPNPKFDAVAGYHWFDGDGMIHGVRIKDGKATYVSRYVKTSRLKQ 127

Query: 134 EEHFGGAKFMKIGDLKGFFGLIMVNMQMLRAKLKVLDVSYGTGTSNTALIYHHGKLLALS 193
           EE FG AKFMKIGDLKGFFGL+MVN+Q LR KLK+LD +YG GT+NTAL+YHHGKLLAL 
Sbjct: 128 EEFFGAAKFMKIGDLKGFFGLLMVNVQQLRTKLKILDNTYGNGTANTALVYHHGKLLALQ 187

Query: 194 EADKPYVIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPVTGEMFTFGYSHSPPYVTYR 253
           EADKPYVIKVLEDGDLQTLG++DYDKRLTHSFTAHPKVDPVTGEMFTFGYSH+PPY+TYR
Sbjct: 188 EADKPYVIKVLEDGDLQTLGIIDYDKRLTHSFTAHPKVDPVTGEMFTFGYSHTPPYLTYR 247

Query: 254 VISKDGLMHDPVPITIPNPIMMHDFAITENYAIFMDLPLYFKPKEMVKENKLIFTFDATK 313
           VISKDG+MHDPVPITI  PIMMHDFAITE YAIFMDLP++F+PKEMVKE K+I++FD TK
Sbjct: 248 VISKDGIMHDPVPITISEPIMMHDFAITETYAIFMDLPMHFRPKEMVKEKKMIYSFDPTK 307

Query: 314 RARFGVLPRYARDDLLMRWFELPNCFIFHNANAWEEGDEVVLITCRLENPDLDMVSGSVK 373
           +ARFGVLPRYA+D+L++RWFELPNCFIFHNANAWEE DEVVLITCRLENPDLDMVSG VK
Sbjct: 308 KARFGVLPRYAKDELMIRWFELPNCFIFHNANAWEEEDEVVLITCRLENPDLDMVSGKVK 367

Query: 374 EKLENFANELYEMRFNLKSGLASQKKLSESAVDFPRVNEWYTGR----YFNSLIFLV--- 433
           EKLENF NELYEMRFN+K+G ASQKKLS SAVDFPR+NE YTG+     + +++  +   
Sbjct: 368 EKLENFGNELYEMRFNMKTGSASQKKLSASAVDFPRINECYTGKKQRYVYGTILDSIAKV 427

Query: 434 -----------------------------------FGKEYLLKSMGTYEETSNFGVELLF 493
                                              +G E +     T EE   + +  + 
Sbjct: 428 TGIIKFDLHAEAETGKRMLEVGGNIKGIYDLGEGRYGSEAIYVPRETAEEDDGYLIFFVH 487

BLAST of Spg014656 vs. ExPASy Swiss-Prot
Match: Q84KG5 (Carotenoid 9,10(9',10')-cleavage dioxygenase OS=Crocus sativus OX=82528 GN=CCD PE=1 SV=1)

HSP 1 Score: 765.8 bits (1976), Expect = 3.4e-220
Identity = 381/530 (71.89%), Postives = 422/530 (79.62%), Query Frame = 0

Query: 15  RSLVEVNPKPSKGLASKATDLLEKLFVKLMYDSSKPQHYLSGNFAPVREETPPITDLPVK 74
           RS+V VNP+PSKGL S A DL+EK  V L +D SKP HYLSGNFAPV +ETPP  DLPV+
Sbjct: 13  RSIVAVNPQPSKGLVSSAVDLIEKAVVYLFHDKSKPCHYLSGNFAPVVDETPPCPDLPVR 72

Query: 75  GYLPECLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKATYVSRYVKTSRLKQE 134
           G+LPECLNGEFVRVGPNPKF PVAGYHWFDGDGMIHG+RIKDGKATY SRYVKTSRLKQE
Sbjct: 73  GHLPECLNGEFVRVGPNPKFMPVAGYHWFDGDGMIHGMRIKDGKATYASRYVKTSRLKQE 132

Query: 135 EHFGGAKFMKIGDLKGFFGLIMVNMQMLRAKLKVLDVSYGTGTSNTALIYHHGKLLALSE 194
           E+F G KFMKIGDLKGFFGL MV MQ+LRAKLKV+DVSYG GT NTALIYHHGKLLALSE
Sbjct: 133 EYFEGPKFMKIGDLKGFFGLFMVQMQLLRAKLKVIDVSYGVGTGNTALIYHHGKLLALSE 192

Query: 195 ADKPYVIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPVTGEMFTFGYSHSPPYVTYRV 254
           ADKPYV+KVLEDGDLQTLGLLDYDKRL+HSFTAHPKVDP T EMFTFGY+H+PPYVTYRV
Sbjct: 193 ADKPYVVKVLEDGDLQTLGLLDYDKRLSHSFTAHPKVDPFTDEMFTFGYAHTPPYVTYRV 252

Query: 255 ISKDGLMHDPVPITIPNPIMMHDFAITENYAIFMDLPLYFKPKEMVKENKLIFTFDATKR 314
           ISKDG+M DPVPITIP  +MMHDFAITENY+IFMDLPLYF+PKEMVK  KLIF+FDATK+
Sbjct: 253 ISKDGVMRDPVPITIPASVMMHDFAITENYSIFMDLPLYFQPKEMVKGGKLIFSFDATKK 312

Query: 315 ARFGVLPRYARDDLLMRWFELPNCFIFHNANAWEEGDEVVLITCRLENPDLDMVSGSVKE 374
           ARFGVLPRYA+DD L+RWFELPNCFIFHNANAWEEGDEVVLITCRLENPDLDMV+G+VKE
Sbjct: 313 ARFGVLPRYAKDDSLIRWFELPNCFIFHNANAWEEGDEVVLITCRLENPDLDMVNGAVKE 372

Query: 375 KLENFANELYEMRFNLKSGLASQKKLSESAVDFPRVNEWYT------------------- 434
           KLENF NELYEMRFN+K+G ASQK+LS SAVDFPR+NE YT                   
Sbjct: 373 KLENFKNELYEMRFNMKTGAASQKQLSVSAVDFPRINESYTTRKQRYVYGTILDNITKVK 432

Query: 435 --------------------------------GRYFNSLIFLVFGKEYLLKSMGTYEETS 494
                                           GRY +  +F+   +E  +KS    EE  
Sbjct: 433 GIIKFDLHAEPEAGKKKLEVGGNVQGIFDLGPGRYGSEAVFV--PRERGIKS----EEDD 492

BLAST of Spg014656 vs. ExPASy Swiss-Prot
Match: Q8LP17 (Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Pisum sativum OX=3888 GN=CCD1 PE=2 SV=1)

HSP 1 Score: 748.4 bits (1931), Expect = 5.7e-215
Identity = 369/522 (70.69%), Postives = 418/522 (80.08%), Query Frame = 0

Query: 17  LVEVNPKPSKGLASKATDLLEKLFVKLMYDSSKPQHYLSGNFAPVREETPPITDLPVKGY 76
           ++EV PKPS G  SKA DLLEK+ VKL YDSS P H+LSGNFAPV++ETPP+ DL V+G+
Sbjct: 11  ILEVEPKPSNGFTSKAVDLLEKIIVKLFYDSSLPHHWLSGNFAPVKDETPPVKDLTVQGH 70

Query: 77  LPECLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKATYVSRYVKTSRLKQEEH 136
           LP+CLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKA+YVSR+VKTSR KQEE+
Sbjct: 71  LPDCLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKASYVSRFVKTSRFKQEEY 130

Query: 137 FGGAKFMKIGDLKGFFGLIMVNMQMLRAKLKVLDVSYGTGTSNTALIYHHGKLLALSEAD 196
           F G+KFMKIGDLKG FGL+MVNMQMLRAKLK+LDVSYG GT+NTAL+YHH KLLALSE D
Sbjct: 131 FNGSKFMKIGDLKGLFGLLMVNMQMLRAKLKILDVSYGHGTANTALVYHHQKLLALSEGD 190

Query: 197 KPYVIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPVTGEMFTFGYSHSPPYVTYRVIS 256
           KPY IKV EDGDLQTLG+LDYDKRL H+FTAHPKVDP TGEMFTFGYSH+ PYVTYRVIS
Sbjct: 191 KPYAIKVFEDGDLQTLGMLDYDKRLGHNFTAHPKVDPFTGEMFTFGYSHTAPYVTYRVIS 250

Query: 257 KDGLMHDPVPITIPNPIMMHDFAITENYAIFMDLPLYFKPKEMVKENKLIFTFDATKRAR 316
           KDG M DPVPITI +P+MMHDFAITENY+IFMDLPLYF+PKEMVK   LIF+FD+TK+AR
Sbjct: 251 KDGFMQDPVPITISDPVMMHDFAITENYSIFMDLPLYFRPKEMVKNKTLIFSFDSTKKAR 310

Query: 317 FGVLPRYARDDLLMRWFELPNCFIFHNANAWEEGDEVVLITCRLENPDLDMVSGSVKEKL 376
           FGVLPRYA+DD  +RWFELPNCFIFHNANAWEE DE+VLITCRLENP+LD+V G+VKEKL
Sbjct: 311 FGVLPRYAKDDKHIRWFELPNCFIFHNANAWEEEDEIVLITCRLENPNLDVVGGAVKEKL 370

Query: 377 ENFANELYEMRFNLKSGLASQKKLSESAVDFPRVNEWYTGR-----YFNSLIFLV----- 436
           +NF+NELYEMRFN+K+G ASQKKLS S VDFPRVNE YTGR     Y  +L  +      
Sbjct: 371 DNFSNELYEMRFNMKTGEASQKKLSASTVDFPRVNESYTGRKQRYVYGTTLDSIAKVTGI 430

Query: 437 --------------------------------FGKE--YLLKSMGT-YEETSNFGVELLF 494
                                           FG E  Y+ +  GT  EE   + +  + 
Sbjct: 431 IKFDLHAEPDSGKTKLEVGGNVQGLYDLGPGRFGSEAVYVPRVPGTDSEEDDGYLIFFVH 490

BLAST of Spg014656 vs. ExPASy Swiss-Prot
Match: Q94IR2 (Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Phaseolus vulgaris OX=3885 GN=CCD1 PE=1 SV=1)

HSP 1 Score: 741.5 bits (1913), Expect = 7.0e-213
Identity = 372/532 (69.92%), Postives = 422/532 (79.32%), Query Frame = 0

Query: 7   KLNGGVGGRSLVEVNPKPSKGLASKATDLLEKLFVKLMYDSSKPQHYLSGNFAPVREETP 66
           K NG  GG  LV+V+PKP+ G +SK  DLLEKL VK +YDSS P HYL+GNF PV  ETP
Sbjct: 6   KKNGAEGG--LVKVDPKPTNGFSSKVIDLLEKLLVKFLYDSSLPHHYLTGNFGPV-TETP 65

Query: 67  PITDLPVKGYLPECLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKATYVSRYV 126
           P  DLPVKG+LP+CLNGEFVRVGPNPKF+PVAGYHWFDGDGMIHGLRIKDGKATYVSR+V
Sbjct: 66  PTKDLPVKGHLPDCLNGEFVRVGPNPKFAPVAGYHWFDGDGMIHGLRIKDGKATYVSRFV 125

Query: 127 KTSRLKQEEHFGGAKFMKIGDLKGFFGLIMVNMQMLRAKLKVLDVSYGTGTSNTALIYHH 186
           +TSRLKQEE+FG +KFMKIGDLKG FGL+MVN+ MLR KLKVLD+SYG GT+NTAL+YHH
Sbjct: 126 ETSRLKQEEYFGRSKFMKIGDLKGLFGLLMVNIHMLRTKLKVLDLSYGGGTTNTALVYHH 185

Query: 187 GKLLALSEADKPYVIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPVTGEMFTFGYSHS 246
           GKLLALSEADKPY IKV EDGDLQTLG+LDYDKRL HSFTAHPKVDP TGEMF+FGY+H+
Sbjct: 186 GKLLALSEADKPYAIKVFEDGDLQTLGMLDYDKRLGHSFTAHPKVDPFTGEMFSFGYAHT 245

Query: 247 PPYVTYRVISKDGLMHDPVPITIPNPIMMHDFAITENYAIFMDLPLYFKPKEMVKENKLI 306
           PPY+TYRVISKDG MHDPVPITI +PIMMHDFAITENYA+FMDLPL F+PKEMVK   LI
Sbjct: 246 PPYITYRVISKDGYMHDPVPITISDPIMMHDFAITENYAVFMDLPLIFRPKEMVKNKTLI 305

Query: 307 FTFDATKRARFGVLPRYARDDLLMRWFELPNCFIFHNANAWEEGDEVVLITCRLENPDLD 366
           F+FD+TK+ARFGVLPRYA+D+  +RWFELPNCFIFHNANAWEE DEVVLITCRL+NP LD
Sbjct: 306 FSFDSTKKARFGVLPRYAKDEQHIRWFELPNCFIFHNANAWEEEDEVVLITCRLQNPKLD 365

Query: 367 MVSGSVKEKLENFANELYEMRFNLKSGLASQKKLSESAVDFPRVNEWYTGR-----YFNS 426
            V G+V+EKLENF+NELYEMRFN+K+G ASQKKLS S VDFPRVNE YTGR     Y  +
Sbjct: 366 NVGGTVQEKLENFSNELYEMRFNMKTGEASQKKLSASTVDFPRVNENYTGRKQRYVYGTT 425

Query: 427 LIFLV-------------------------------------FGKE--YLLKSMG-TYEE 486
           L  +                                      FG E  Y+ +  G   EE
Sbjct: 426 LDSIAKVTGIIKFDLHAEPDHGKEKLEVGGNVQGLYDLGPGKFGSEAVYIPRVPGIESEE 485

Query: 487 TSNFGVELLFKADSRKSVVNVIDAKTMSSEPVAVVELPHRVPYGFHAFFVTE 494
              + V  +   ++ KS V+VIDAKTMS++PVAVVELP+RVPYGFHAFFVTE
Sbjct: 486 DDGYLVLFVHDENAGKSFVHVIDAKTMSADPVAVVELPNRVPYGFHAFFVTE 534

BLAST of Spg014656 vs. ExPASy Swiss-Prot
Match: C3VEQ4 (Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Oncidium hybrid cultivar OX=141207 GN=CCD1 PE=2 SV=1)

HSP 1 Score: 717.6 bits (1851), Expect = 1.1e-205
Identity = 361/535 (67.48%), Postives = 416/535 (77.76%), Query Frame = 0

Query: 4   EKQKLNGGVGGRSLVEVNPKPSKGLASKATDLLEKLFVKLMYDSSKPQHYLSGNFAPVRE 63
           E++  +G    RS+V V PKP KG+ S   DL+EK+ V   +DSSKP HYL+GNFAPVR+
Sbjct: 19  EEEAADGKKSHRSVVAVQPKPRKGILSSTIDLIEKVIVYFAHDSSKPLHYLTGNFAPVRD 78

Query: 64  ETPPITDLPVKGYLPECLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKATYVS 123
           ETPP   L V G LP CLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKATYVS
Sbjct: 79  ETPPFAALTVHGSLPVCLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKATYVS 138

Query: 124 RYVKTSRLKQEEHFGGAKFMKIGDLKGFFGLIMVNMQMLRAKLKVLDVSYGTGTSNTALI 183
           RYVKTS+L QEE+F GAKFMKIGDLKG FGL MV M++LRAKLK+LDVSYG  T+NTA+I
Sbjct: 139 RYVKTSKLMQEEYFEGAKFMKIGDLKGLFGLFMVQMELLRAKLKILDVSYGRSTANTAMI 198

Query: 184 YHHGKLLALSEADKPYVIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPVTGEMFTFGY 243
           YHHGKLLALSE DKPYVIKVLEDGDLQTLGLLDY+KRL HSFTAHPKVDP T EMFTFGY
Sbjct: 199 YHHGKLLALSEGDKPYVIKVLEDGDLQTLGLLDYNKRLAHSFTAHPKVDPFTDEMFTFGY 258

Query: 244 SHSPPYVTYRVISKDGLMHDPVPITIPNPIMMHDFAITENYAIFMDLPLYFKPKEMVKEN 303
           SH+ PY+TYRVI+KDG+M +PVPITIP PIMMHDFAITENYAIF+DLPLYF+PKE +K  
Sbjct: 259 SHTSPYITYRVITKDGVMLNPVPITIPEPIMMHDFAITENYAIFLDLPLYFRPKETIK-G 318

Query: 304 KLIFTFDATKRARFGVLPRYARDDLLMRWFELPNCFIFHNANAWEEGDEVVLITCRLENP 363
           KLIFTFD TK+A+FGVLPRYA+D+ L+RWF+LPNCFIFHNANAWE+GDEVVLITCRL+N 
Sbjct: 319 KLIFTFDPTKKAKFGVLPRYAKDEQLIRWFDLPNCFIFHNANAWEDGDEVVLITCRLKNI 378

Query: 364 DLDMVSGSVKEKLENFANELYEMRFNLKSGLASQKKLSESAVDFPRVNEWYTGR-----Y 423
           DLD+V+G+VKEKLENF+N+LYEMRFN+K+G A+QK+LS   VDFPR+NE YTGR     Y
Sbjct: 379 DLDLVNGAVKEKLENFSNQLYEMRFNMKTGAATQKELSAPMVDFPRINENYTGRKQRYVY 438

Query: 424 FNSL---------------------------------IFLV----FGKE--YLLKSMGT- 483
             S                                  IF +    FG E  ++ +  GT 
Sbjct: 439 CTSFANIDKVNGIVKFDLHAEPETGKKVLEVGGNVLGIFDLGPGRFGSEAVFVPREAGTR 498

Query: 484 YEETSNFGVELLFKADSRKSVVNVIDAKTMSSEPVAVVELPHRVPYGFHAFFVTE 494
            EE   + +  ++   + +S V VIDAKTMS EPVAVV+LP RVPYGFHAFFV E
Sbjct: 499 VEEDDGYLIFFVYDETTGESKVYVIDAKTMSPEPVAVVDLPCRVPYGFHAFFVNE 552

BLAST of Spg014656 vs. ExPASy TrEMBL
Match: A0A6J1D5G1 (carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Momordica charantia OX=3673 GN=LOC111017209 PE=3 SV=1)

HSP 1 Score: 886.3 bits (2289), Expect = 6.5e-254
Identity = 445/538 (82.71%), Postives = 464/538 (86.25%), Query Frame = 0

Query: 1   MAEEKQKLNGGVGGRSLVEVNPKPSKGLASKATDLLEKLFVKLMYDSSKPQHYLSGNFAP 60
           MAEEKQKLNGGV  RSLVEVNPKPSKGLASKATDLLEKLFVKLMYD+S PQHYLSGNFAP
Sbjct: 1   MAEEKQKLNGGVVDRSLVEVNPKPSKGLASKATDLLEKLFVKLMYDASNPQHYLSGNFAP 60

Query: 61  VREETPPITDLPVKGYLPECLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKAT 120
           VR+ETPPITDLPVKGYLPECLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKAT
Sbjct: 61  VRDETPPITDLPVKGYLPECLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKAT 120

Query: 121 YVSRYVKTSRLKQEEHFGGAKFMKIGDLKGFFGLIMVNMQMLRAKLKVLDVSYGTGTSNT 180
           YVSRYVKTSRLKQEE+FGGAKFMKIGDLKGFFGLIMVNMQMLRAKLKVLDVSYGTGT NT
Sbjct: 121 YVSRYVKTSRLKQEEYFGGAKFMKIGDLKGFFGLIMVNMQMLRAKLKVLDVSYGTGTGNT 180

Query: 181 ALIYHHGKLLALSEADKPYVIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPVTGEMFT 240
           ALIYHHGKLLALSEADKPYVIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPVTGEMFT
Sbjct: 181 ALIYHHGKLLALSEADKPYVIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPVTGEMFT 240

Query: 241 FGYSHSPPYVTYRVISKDGLMHDPVPITIPNPIMMHDFAITENYAIFMDLPLYFKPKEMV 300
           FGYSHSPPYVTYRVISKDGLMHDP+PITIPNP+MMHDFAITENYAIFMDLPLYFKPKEMV
Sbjct: 241 FGYSHSPPYVTYRVISKDGLMHDPIPITIPNPVMMHDFAITENYAIFMDLPLYFKPKEMV 300

Query: 301 KENKLIFTFDATKRARFGVLPRYARDDLLMRWFELPNCFIFHNANAWEEGDEVVLITCRL 360
           KENKLIFTFDATKRARFGVLPRYARDDLL+RWFELPNCFIFHNANAWEEGDEVVLITCRL
Sbjct: 301 KENKLIFTFDATKRARFGVLPRYARDDLLIRWFELPNCFIFHNANAWEEGDEVVLITCRL 360

Query: 361 ENPDLDMVSGSVKEKLENFANELYEMRFNLKSGLASQKKLSESAVDFPRVNEWYTGR--- 420
           ENPDLDMVSGSVKEKLENF+NELYEMRFNLKSG ASQKKLSESAVDFPRVNE YTGR   
Sbjct: 361 ENPDLDMVSGSVKEKLENFSNELYEMRFNLKSGRASQKKLSESAVDFPRVNESYTGRKQQ 420

Query: 421 -YFNSLIFLV--------------------------------------FGKEYLLKSM-- 480
             + +++  +                                      FG E +      
Sbjct: 421 YVYGTILDSIAKVTGIAKFDLNAKPETGKTKIEVGGNVQGLYDLGPGRFGSEAIFVPRIP 480

Query: 481 -GTYEETSNFGVELLFKADSRKSVVNVIDAKTMSSEPVAVVELPHRVPYGFHAFFVTE 494
             T EE   + +  +   ++ KS VNVIDAKTMS+EPVAVVELPHRVPYGFHAFFVTE
Sbjct: 481 GTTSEEDDGYLIFFVHDENTGKSSVNVIDAKTMSTEPVAVVELPHRVPYGFHAFFVTE 538

BLAST of Spg014656 vs. ExPASy TrEMBL
Match: K4K0F3 (Carotenoid cleavage dioxygenase 1 OS=Momordica charantia OX=3673 GN=CCD1 PE=2 SV=1)

HSP 1 Score: 885.2 bits (2286), Expect = 1.4e-253
Identity = 444/538 (82.53%), Postives = 464/538 (86.25%), Query Frame = 0

Query: 1   MAEEKQKLNGGVGGRSLVEVNPKPSKGLASKATDLLEKLFVKLMYDSSKPQHYLSGNFAP 60
           MAEEKQKLNGGV  RSLVEVNPKPSKGLASKATDLLEKLFVKLMYD+S PQHYLSGNFAP
Sbjct: 1   MAEEKQKLNGGVVDRSLVEVNPKPSKGLASKATDLLEKLFVKLMYDASNPQHYLSGNFAP 60

Query: 61  VREETPPITDLPVKGYLPECLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKAT 120
           VR+ETPPITDLPVKGYLPECLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKAT
Sbjct: 61  VRDETPPITDLPVKGYLPECLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKAT 120

Query: 121 YVSRYVKTSRLKQEEHFGGAKFMKIGDLKGFFGLIMVNMQMLRAKLKVLDVSYGTGTSNT 180
           YVSRYVKTSRLKQEE+FGGAKFMKIGDLKGFFGLIMVNMQMLRAKLKVLDVSYGTGT NT
Sbjct: 121 YVSRYVKTSRLKQEEYFGGAKFMKIGDLKGFFGLIMVNMQMLRAKLKVLDVSYGTGTGNT 180

Query: 181 ALIYHHGKLLALSEADKPYVIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPVTGEMFT 240
           ALIYHHGKLLALSEADKPYVIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPVTGEMFT
Sbjct: 181 ALIYHHGKLLALSEADKPYVIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPVTGEMFT 240

Query: 241 FGYSHSPPYVTYRVISKDGLMHDPVPITIPNPIMMHDFAITENYAIFMDLPLYFKPKEMV 300
           FGYSHSPPYVTYRVISKDGLMHDP+PITIPNP+MMHDFAITENYAIFMDLPLYFKPKE+V
Sbjct: 241 FGYSHSPPYVTYRVISKDGLMHDPIPITIPNPVMMHDFAITENYAIFMDLPLYFKPKELV 300

Query: 301 KENKLIFTFDATKRARFGVLPRYARDDLLMRWFELPNCFIFHNANAWEEGDEVVLITCRL 360
           KENKLIFTFDATKRARFGVLPRYARDDLL+RWFELPNCFIFHNANAWEEGDEVVLITCRL
Sbjct: 301 KENKLIFTFDATKRARFGVLPRYARDDLLIRWFELPNCFIFHNANAWEEGDEVVLITCRL 360

Query: 361 ENPDLDMVSGSVKEKLENFANELYEMRFNLKSGLASQKKLSESAVDFPRVNEWYTGR--- 420
           ENPDLDMVSGSVKEKLENF+NELYEMRFNLKSG ASQKKLSESAVDFPRVNE YTGR   
Sbjct: 361 ENPDLDMVSGSVKEKLENFSNELYEMRFNLKSGRASQKKLSESAVDFPRVNESYTGRKQQ 420

Query: 421 -YFNSLIFLV--------------------------------------FGKEYLLKSM-- 480
             + +++  +                                      FG E +      
Sbjct: 421 YVYGTILDSIAKVTGIAKFDLNAKPETGKTKIEVGGNVQGLYDPGPGRFGSEAIFVPRIP 480

Query: 481 -GTYEETSNFGVELLFKADSRKSVVNVIDAKTMSSEPVAVVELPHRVPYGFHAFFVTE 494
             T EE   + +  +   ++ KS VNVIDAKTMS+EPVAVVELPHRVPYGFHAFFVTE
Sbjct: 481 GTTSEEDDGYLIFFVHDENTGKSSVNVIDAKTMSTEPVAVVELPHRVPYGFHAFFVTE 538

BLAST of Spg014656 vs. ExPASy TrEMBL
Match: A0A6J1JU53 (carotenoid 9,10(9',10')-cleavage dioxygenase 1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111488382 PE=3 SV=1)

HSP 1 Score: 881.3 bits (2276), Expect = 2.1e-252
Identity = 444/539 (82.37%), Postives = 466/539 (86.46%), Query Frame = 0

Query: 1   MAEEKQKLNGGVGGRSLVEVNPKPSKGLASKATDLLEKLFVKLMYDSSKPQHYLSGNFAP 60
           MAEEKQ+LNGGV G SLVEVNPKPSKGLASK TDLLEKLFVKLMYDSSKPQHYLSGNFAP
Sbjct: 1   MAEEKQRLNGGVVGGSLVEVNPKPSKGLASKVTDLLEKLFVKLMYDSSKPQHYLSGNFAP 60

Query: 61  VREETPPITDLPVKGYLPECLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKAT 120
           V +ETPPITDLPVKGYLPECLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKAT
Sbjct: 61  VGDETPPITDLPVKGYLPECLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKAT 120

Query: 121 YVSRYVKTSRLKQEEHFGGAKFMKIGDLKGFFGLIMVNMQMLRAKLKVLDVSYGTGTSNT 180
           YVSRYV+TSRLKQEE+FGGAKFMKIGDLKGFFGL+MVNMQ+LRAKLKVLDVSYGTGT NT
Sbjct: 121 YVSRYVRTSRLKQEEYFGGAKFMKIGDLKGFFGLVMVNMQILRAKLKVLDVSYGTGTGNT 180

Query: 181 ALIYHHGKLLALSEADKPYVIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPVTGEMFT 240
           ALIYHHGKLLALSEADKPYVIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPVTGEMFT
Sbjct: 181 ALIYHHGKLLALSEADKPYVIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPVTGEMFT 240

Query: 241 FGYSHSPPYVTYRVISKDGLMHDPVPITIPNPIMMHDFAITENYAIFMDLPLYFKPKEMV 300
           FGYSH+PPYVTYRVISKDGLMHDPVPITIPNP+MMHDFAITENYAIFMDLPLYFKPKEMV
Sbjct: 241 FGYSHTPPYVTYRVISKDGLMHDPVPITIPNPVMMHDFAITENYAIFMDLPLYFKPKEMV 300

Query: 301 KENKLIFTFDATKRARFGVLPRYARDDLLMRWFELPNCFIFHNANAWEEGDEVVLITCRL 360
           KENKLIFTFDATK+ARFGVLPRYA+DDLLMRWFELPNCFIFHNANAWEEGDEVVLITCRL
Sbjct: 301 KENKLIFTFDATKKARFGVLPRYAKDDLLMRWFELPNCFIFHNANAWEEGDEVVLITCRL 360

Query: 361 ENPDLDMVSGSVKEKLENFANELYEMRFNLKSGLASQKKLSESAVDFPRVNEWYTGR--- 420
           ENPDLDMVSGSVKEKLENF+NELYEMRFNLKSGL+SQ+KLSESAVDFPRVNE YTGR   
Sbjct: 361 ENPDLDMVSGSVKEKLENFSNELYEMRFNLKSGLSSQRKLSESAVDFPRVNECYTGRKQR 420

Query: 421 --YFNSLIFLV-------------------------------------FGKE--YLLKSM 480
             Y  +L  +                                      FG E  ++ +  
Sbjct: 421 YVYGTTLDSIAKVTGIAKFDLHAKRETGRTKIEVGGNVQGLYDLGPGRFGSEAIFVPRIP 480

Query: 481 GTYEETSNFGVELLFKADSR--KSVVNVIDAKTMSSEPVAVVELPHRVPYGFHAFFVTE 494
           GT  E  + G  +LF  D    KS VNV+DAKTMSSEPVAVVELPHRVPYGFHAFFV+E
Sbjct: 481 GTTSEEDD-GYLILFVHDENTGKSAVNVVDAKTMSSEPVAVVELPHRVPYGFHAFFVSE 538

BLAST of Spg014656 vs. ExPASy TrEMBL
Match: A0A6J1GNN0 (carotenoid 9,10(9',10')-cleavage dioxygenase 1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456066 PE=3 SV=1)

HSP 1 Score: 881.3 bits (2276), Expect = 2.1e-252
Identity = 444/539 (82.37%), Postives = 466/539 (86.46%), Query Frame = 0

Query: 1   MAEEKQKLNGGVGGRSLVEVNPKPSKGLASKATDLLEKLFVKLMYDSSKPQHYLSGNFAP 60
           MAEEKQ+LNGGV G SLVEVNPKPSKGLASK TDLLEKLFVKLMYDSSKPQHYLSGNFAP
Sbjct: 1   MAEEKQRLNGGVVGGSLVEVNPKPSKGLASKVTDLLEKLFVKLMYDSSKPQHYLSGNFAP 60

Query: 61  VREETPPITDLPVKGYLPECLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKAT 120
           V +ETPPITDLPVKGYLPECLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKAT
Sbjct: 61  VGDETPPITDLPVKGYLPECLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKAT 120

Query: 121 YVSRYVKTSRLKQEEHFGGAKFMKIGDLKGFFGLIMVNMQMLRAKLKVLDVSYGTGTSNT 180
           YVSRYV+TSRLKQEE+FGGAKFMKIGDLKGFFGL+MVNMQ+LRAKLKVLDVSYGTGT NT
Sbjct: 121 YVSRYVRTSRLKQEEYFGGAKFMKIGDLKGFFGLVMVNMQILRAKLKVLDVSYGTGTGNT 180

Query: 181 ALIYHHGKLLALSEADKPYVIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPVTGEMFT 240
           ALIYHHGKLLALSEADKPYVIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPVTGEMFT
Sbjct: 181 ALIYHHGKLLALSEADKPYVIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPVTGEMFT 240

Query: 241 FGYSHSPPYVTYRVISKDGLMHDPVPITIPNPIMMHDFAITENYAIFMDLPLYFKPKEMV 300
           FGYSH+PPYVTYRVISKDGLMHDPVPITIPNP+MMHDFAITENYAIFMDLPLYFKPKEMV
Sbjct: 241 FGYSHTPPYVTYRVISKDGLMHDPVPITIPNPVMMHDFAITENYAIFMDLPLYFKPKEMV 300

Query: 301 KENKLIFTFDATKRARFGVLPRYARDDLLMRWFELPNCFIFHNANAWEEGDEVVLITCRL 360
           KENKLIFTFDATK+ARFGVLPRYA+DDLLMRWFELPNCFIFHNANAWEEGDEVVLITCRL
Sbjct: 301 KENKLIFTFDATKKARFGVLPRYAKDDLLMRWFELPNCFIFHNANAWEEGDEVVLITCRL 360

Query: 361 ENPDLDMVSGSVKEKLENFANELYEMRFNLKSGLASQKKLSESAVDFPRVNEWYTGR--- 420
           ENPDLDMVSGSVKEKLENF+NELYEMRFNLKSGL+SQ+KLSESAVDFPRVNE YTGR   
Sbjct: 361 ENPDLDMVSGSVKEKLENFSNELYEMRFNLKSGLSSQRKLSESAVDFPRVNECYTGRKQR 420

Query: 421 --YFNSLIFLV-------------------------------------FGKE--YLLKSM 480
             Y  +L  +                                      FG E  ++ +  
Sbjct: 421 YVYGTTLDSIAKVTGIAKFDLHAKRETGRTKIEVGGNVRGLYDLGPGRFGSEAIFVPRIP 480

Query: 481 GTYEETSNFGVELLFKADSR--KSVVNVIDAKTMSSEPVAVVELPHRVPYGFHAFFVTE 494
           GT  E  + G  +LF  D    KS VNV+DAKTMSSEPVAVVELPHRVPYGFHAFFV+E
Sbjct: 481 GTTSEEDD-GYLILFVHDENTGKSAVNVVDAKTMSSEPVAVVELPHRVPYGFHAFFVSE 538

BLAST of Spg014656 vs. ExPASy TrEMBL
Match: A0A1S3CFM3 (carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Cucumis melo OX=3656 GN=LOC103499930 PE=3 SV=1)

HSP 1 Score: 873.6 bits (2256), Expect = 4.3e-250
Identity = 440/539 (81.63%), Postives = 464/539 (86.09%), Query Frame = 0

Query: 1   MAEEKQKLNGGVGGRSLVEVNPKPSKGLASKATDLLEKLFVKLMYDSSKPQHYLSGNFAP 60
           MA+EKQKLNGG+ GRSLVEV+PKPSKGL SKATDLLEKLFVKLMYDSSKPQHYLSGNFAP
Sbjct: 1   MADEKQKLNGGLPGRSLVEVSPKPSKGLVSKATDLLEKLFVKLMYDSSKPQHYLSGNFAP 60

Query: 61  VREETPPITDLPVKGYLPECLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKAT 120
           V +ETPPITDLPVKGYLPECLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKA+
Sbjct: 61  VHDETPPITDLPVKGYLPECLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKAS 120

Query: 121 YVSRYVKTSRLKQEEHFGGAKFMKIGDLKGFFGLIMVNMQMLRAKLKVLDVSYGTGTSNT 180
           YVSRYV+TSRLKQEE+FG AKFMKIGDLKGFFGLIMVNMQMLRAKLKVLDVSYGTGT NT
Sbjct: 121 YVSRYVRTSRLKQEEYFGAAKFMKIGDLKGFFGLIMVNMQMLRAKLKVLDVSYGTGTGNT 180

Query: 181 ALIYHHGKLLALSEADKPYVIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPVTGEMFT 240
           ALIYHHGKLLALSE DKPYVIKVLEDGDLQTLGLLDYDKRLTH+FTAHPKVDPVTGEMFT
Sbjct: 181 ALIYHHGKLLALSEGDKPYVIKVLEDGDLQTLGLLDYDKRLTHTFTAHPKVDPVTGEMFT 240

Query: 241 FGYSHSPPYVTYRVISKDGLMHDPVPITIPNPIMMHDFAITENYAIFMDLPLYFKPKEMV 300
           FGYSHSPPYVTYRVISKDGLMHDPVPITIP P+MMHDFAITENYAIFMDLPLYFKPKEMV
Sbjct: 241 FGYSHSPPYVTYRVISKDGLMHDPVPITIPAPVMMHDFAITENYAIFMDLPLYFKPKEMV 300

Query: 301 KENKLIFTFDATKRARFGVLPRYARDDLLMRWFELPNCFIFHNANAWEEGDEVVLITCRL 360
           KENKLIFTFDATK+ARFGVLPRYA+DDLL++WFELPNCFIFHNANAWEEGDE+VLITCRL
Sbjct: 301 KENKLIFTFDATKKARFGVLPRYAKDDLLIQWFELPNCFIFHNANAWEEGDELVLITCRL 360

Query: 361 ENPDLDMVSGSVKEKLENFANELYEMRFNLKSGLASQKKLSESAVDFPRVNEWYTGR--- 420
           ENPDLDMVSGSVKEKLENF+NELYEMRFNLKSGLASQ+KLSESAVDFPRVNE YTGR   
Sbjct: 361 ENPDLDMVSGSVKEKLENFSNELYEMRFNLKSGLASQRKLSESAVDFPRVNESYTGRKQQ 420

Query: 421 --YFNSLIFLV-------------------------------------FGKE--YLLKSM 480
             Y  +L  +                                      FG E  ++ +  
Sbjct: 421 YVYGTTLDSIAKVTGIAKFDLHAEPETGKTKIEVGGNVQGLYDLGPGRFGSEAIFVPRVP 480

Query: 481 GTYEETSNFGVELLFKADSR--KSVVNVIDAKTMSSEPVAVVELPHRVPYGFHAFFVTE 494
           GT  E  + G  +LF  D    KS VNV+DAKTMSSEPVAVVELPHRVPYGFHAFFVTE
Sbjct: 481 GTTSEEDD-GYLILFVHDENTGKSAVNVVDAKTMSSEPVAVVELPHRVPYGFHAFFVTE 538

BLAST of Spg014656 vs. TAIR 10
Match: AT3G63520.1 (carotenoid cleavage dioxygenase 1 )

HSP 1 Score: 775.4 bits (2001), Expect = 3.1e-224
Identity = 373/522 (71.46%), Postives = 425/522 (81.42%), Query Frame = 0

Query: 14  GRSLVEVNPKPSKGLASKATDLLEKLFVKLMYDSSKPQHYLSGNFAPVREETPPITDLPV 73
           G S++ V+P+PSKG +SK  DLLE+L VKLM+D+S P HYLSGNFAP+R+ETPP+ DLPV
Sbjct: 8   GSSIISVHPRPSKGFSSKLLDLLERLVVKLMHDASLPLHYLSGNFAPIRDETPPVKDLPV 67

Query: 74  KGYLPECLNGEFVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKATYVSRYVKTSRLKQ 133
            G+LPECLNGEFVRVGPNPKF  VAGYHWFDGDGMIHG+RIKDGKATYVSRYVKTSRLKQ
Sbjct: 68  HGFLPECLNGEFVRVGPNPKFDAVAGYHWFDGDGMIHGVRIKDGKATYVSRYVKTSRLKQ 127

Query: 134 EEHFGGAKFMKIGDLKGFFGLIMVNMQMLRAKLKVLDVSYGTGTSNTALIYHHGKLLALS 193
           EE FG AKFMKIGDLKGFFGL+MVN+Q LR KLK+LD +YG GT+NTAL+YHHGKLLAL 
Sbjct: 128 EEFFGAAKFMKIGDLKGFFGLLMVNVQQLRTKLKILDNTYGNGTANTALVYHHGKLLALQ 187

Query: 194 EADKPYVIKVLEDGDLQTLGLLDYDKRLTHSFTAHPKVDPVTGEMFTFGYSHSPPYVTYR 253
           EADKPYVIKVLEDGDLQTLG++DYDKRLTHSFTAHPKVDPVTGEMFTFGYSH+PPY+TYR
Sbjct: 188 EADKPYVIKVLEDGDLQTLGIIDYDKRLTHSFTAHPKVDPVTGEMFTFGYSHTPPYLTYR 247

Query: 254 VISKDGLMHDPVPITIPNPIMMHDFAITENYAIFMDLPLYFKPKEMVKENKLIFTFDATK 313
           VISKDG+MHDPVPITI  PIMMHDFAITE YAIFMDLP++F+PKEMVKE K+I++FD TK
Sbjct: 248 VISKDGIMHDPVPITISEPIMMHDFAITETYAIFMDLPMHFRPKEMVKEKKMIYSFDPTK 307

Query: 314 RARFGVLPRYARDDLLMRWFELPNCFIFHNANAWEEGDEVVLITCRLENPDLDMVSGSVK 373
           +ARFGVLPRYA+D+L++RWFELPNCFIFHNANAWEE DEVVLITCRLENPDLDMVSG VK
Sbjct: 308 KARFGVLPRYAKDELMIRWFELPNCFIFHNANAWEEEDEVVLITCRLENPDLDMVSGKVK 367

Query: 374 EKLENFANELYEMRFNLKSGLASQKKLSESAVDFPRVNEWYTGR----YFNSLIFLV--- 433
           EKLENF NELYEMRFN+K+G ASQKKLS SAVDFPR+NE YTG+     + +++  +   
Sbjct: 368 EKLENFGNELYEMRFNMKTGSASQKKLSASAVDFPRINECYTGKKQRYVYGTILDSIAKV 427

Query: 434 -----------------------------------FGKEYLLKSMGTYEETSNFGVELLF 493
                                              +G E +     T EE   + +  + 
Sbjct: 428 TGIIKFDLHAEAETGKRMLEVGGNIKGIYDLGEGRYGSEAIYVPRETAEEDDGYLIFFVH 487

BLAST of Spg014656 vs. TAIR 10
Match: AT3G14440.1 (nine-cis-epoxycarotenoid dioxygenase 3 )

HSP 1 Score: 297.4 bits (760), Expect = 2.5e-80
Identity = 174/483 (36.02%), Postives = 258/483 (53.42%), Query Frame = 0

Query: 47  SSKPQHYLSGNFAPVREETPPITDLPVKGYLPECLNGEFVRVGPNPKFSPVAGYHWFDGD 106
           ++ P   ++GNFAPV E+ P   +LPV G LP+ + G +VR G NP   PV G+H+FDGD
Sbjct: 116 TADPSVQIAGNFAPVNEQ-PVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGHHFFDGD 175

Query: 107 GMIHGLRIKDGKATYVSRYVKTSRLKQEEHFGGAKFMK-IGDLKGFFGLIMVNMQMLRAK 166
           GM+H ++ + G A+Y  R+ +T+R  QE   G   F K IG+L G  G+  + +   RA 
Sbjct: 176 GMVHAVKFEHGSASYACRFTQTNRFVQERQLGRPVFPKAIGELHGHTGIARLMLFYARAA 235

Query: 167 LKVLDVSYGTGTSNTALIYHHGKLLALSEADKPYVIKVLEDGDLQTLGLLDYDKRLTHSF 226
             ++D ++GTG +N  L+Y +G+LLA+SE D PY +++  +GDL+T+G  D+D +L  + 
Sbjct: 236 AGIVDPAHGTGVANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQLESTM 295

Query: 227 TAHPKVDPVTGEMFTFGYS-HSPPYVTYRVISKDGLMHDPVPITIPNPIMMHDFAITENY 286
            AHPKVDP +GE+F   Y   S PY+ Y   S DG     V I +  P MMHDFAITEN+
Sbjct: 296 IAHPKVDPESGELFALSYDVVSKPYLKYFRFSPDGTKSPDVEIQLDQPTMMHDFAITENF 355

Query: 287 AIFMDLPLYFKPKEMVKENKLIFTFDATKRARFGVLPRYARDDLLMRWFELPNCFIFHNA 346
            +  D  + FK  EM++    +  +D  K ARFG+L +YA D   ++W + P+CF FH  
Sbjct: 356 VVVPDQQVVFKLPEMIRGGSPV-VYDKNKVARFGILDKYAEDSSNIKWIDAPDCFCFHLW 415

Query: 347 NAWE--EGDEVVLITCRLENPDLDMVSGSVKEKLENFANELYEMRFNLKSGLASQKKL-- 406
           NAWE  E DEVV+I   +  PD         E  EN  + L E+R NLK+G ++++ +  
Sbjct: 416 NAWEEPETDEVVVIGSCMTPPD-----SIFNESDENLKSVLSEIRLNLKTGESTRRPIIS 475

Query: 407 ---SESAVDFPRVNEWYTGRYFNSLIFLV-----------------------------FG 466
               +  ++   VN    GR        +                             +G
Sbjct: 476 NEDQQVNLEAGMVNRNMLGRKTKFAYLALAEPWPKVSGFAKVDLTTGEVKKHLYGDNRYG 535

Query: 467 KEYLLKSMGTYEETSNFGVELLFKADSRKSVVNVIDAKTMSSEPVAVVELPHRVPYGFHA 492
            E L       EE   + +  +    + KS + +++A  +S E  A V+LP RVPYGFH 
Sbjct: 536 GEPLFLPGEGGEEDEGYILCFVHDEKTWKSELQIVNA--VSLEVEATVKLPSRVPYGFHG 589

BLAST of Spg014656 vs. TAIR 10
Match: AT1G30100.1 (nine-cis-epoxycarotenoid dioxygenase 5 )

HSP 1 Score: 294.7 bits (753), Expect = 1.6e-79
Identity = 179/515 (34.76%), Postives = 273/515 (53.01%), Query Frame = 0

Query: 25  SKGLASKATDLLEKLFVKLMYDSSKPQHYLSGNFAPVREETPPITDLPVKGYLPECLNGE 84
           S  L    T LL +   K +  +  P+H +SGN+APV E++   + L V G +P+C++G 
Sbjct: 84  SAALDFAETALLRRERSKPLPKTVDPRHQISGNYAPVPEQSVK-SSLSVDGKIPDCIDGV 143

Query: 85  FVRVGPNPKFSPVAGYHWFDGDGMIHGLRIKDGKATYVSRYVKTSRLKQEEHFGGAKFMK 144
           ++R G NP F PV+G+H FDGDGM+H ++I +G A+Y  R+ +T RL QE+  G   F K
Sbjct: 144 YLRNGANPLFEPVSGHHLFDGDGMVHAVKITNGDASYSCRFTETERLVQEKQLGSPIFPK 203

Query: 145 -IGDLKGFFGLIMVNMQMLRAKLKVLDVSYGTGTSNTALIYHHGKLLALSEADKPYVIKV 204
            IG+L G  G+  + +   R    +L+   GTG +N  L+Y H +LLA+SE D PY ++V
Sbjct: 204 AIGELHGHSGIARLMLFYARGLFGLLNHKNGTGVANAGLVYFHDRLLAMSEDDLPYQVRV 263

Query: 205 LEDGDLQTLGLLDYDKRLTHSFTAHPKVDPVTGEMFTFGYS-HSPPYVTYRVISKDGLMH 264
            ++GDL+T+G  D+D +L+ +  AHPK+DPVT E+F   Y     PY+ Y   S +G   
Sbjct: 264 TDNGDLETIGRFDFDGQLSSAMIAHPKIDPVTKELFALSYDVVKKPYLKYFKFSPEGEKS 323

Query: 265 DPVPITIPNPIMMHDFAITENYAIFMDLPLYFKPKEMVKENKLIFTFDATKRARFGVLPR 324
             V I + +P MMHDFAITEN+ +  D  + FK  +M    K    +D  K +RFG+LPR
Sbjct: 324 PDVEIPLASPTMMHDFAITENFVVIPDQQVVFKLSDMFL-GKSPVKYDGEKISRFGILPR 383

Query: 325 YARDDLLMRWFELPNCFIFHNANAWE--EGDEVVLITCRLENPDLDMVSGSVKEKLENFA 384
            A+D   M W E P  F FH  NAWE  E DEVV+I   +     D +     E+L +  
Sbjct: 384 NAKDASEMVWVESPETFCFHLWNAWESPETDEVVVIGSCM--TPADSIFNECDEQLNSV- 443

Query: 385 NELYEMRFNLKSGLASQKKLSESAVD---------------------------FPRV--- 444
             L E+R NLK+G ++++ +   +V                            +P+V   
Sbjct: 444 --LSEIRLNLKTGKSTRRTIIPGSVQMNLEAGMVNRNLLGRKTRYAYLAIAEPWPKVSGF 503

Query: 445 -----------NEWYTGRYFNSLIFLVFGKEYLLKSMGTYEETSNFGVELLFKADSRKSV 495
                      N +Y G+ +    F      +L + + +  E   + +  +   +S +S 
Sbjct: 504 AKVDLSTGEVKNHFYGGKKYGGEPF------FLPRGLESDGEDDGYIMSFVHDEESWESE 563

BLAST of Spg014656 vs. TAIR 10
Match: AT1G78390.1 (nine-cis-epoxycarotenoid dioxygenase 9 )

HSP 1 Score: 290.4 bits (742), Expect = 3.0e-78
Identity = 171/482 (35.48%), Postives = 254/482 (52.70%), Query Frame = 0

Query: 47  SSKPQHYLSGNFAPVREETPPITDLPVKGYLPECLNGEFVRVGPNPKFSPVAGYHWFDGD 106
           ++ P   ++GNF PV E+ P + +LPV G +PEC+ G +VR G NP   PV+G+H FDGD
Sbjct: 176 TADPAVQIAGNFFPVPEK-PVVHNLPVTGTVPECIQGVYVRNGANPLHKPVSGHHLFDGD 235

Query: 107 GMIHGLRIKDGKATYVSRYVKTSRLKQEEHFGGAKFMK-IGDLKGFFGLIMVNMQMLRAK 166
           GM+H +R  +G  +Y  R+ +T+RL QE   G   F K IG+L G  G+  + +   R  
Sbjct: 236 GMVHAVRFDNGSVSYACRFTETNRLVQERECGRPVFPKAIGELHGHLGIAKLMLFNTRGL 295

Query: 167 LKVLDVSYGTGTSNTALIYHHGKLLALSEADKPYVIKVLEDGDLQTLGLLDYDKRLTHSF 226
             ++D + G G +N  L+Y +G LLA+SE D PY +KV + GDL+T G  D+D +L  + 
Sbjct: 296 FGLVDPTGGLGVANAGLVYFNGHLLAMSEDDLPYHVKVTQTGDLETSGRYDFDGQLKSTM 355

Query: 227 TAHPKVDPVTGEMFTFGYS-HSPPYVTYRVISKDGLMHDPVPITIPNPIMMHDFAITENY 286
            AHPK+DP T E+F   Y   S PY+ Y   + DG     V I +  P M+HDFAITEN+
Sbjct: 356 IAHPKIDPETRELFALSYDVVSKPYLKYFRFTSDGEKSPDVEIPLDQPTMIHDFAITENF 415

Query: 287 AIFMDLPLYFKPKEMVKENKLIFTFDATKRARFGVLPRYARDDLLMRWFELPNCFIFHNA 346
            +  D  + F+  EM++    +  +D  K++RFG+L + A+D   ++W E+P+CF FH  
Sbjct: 416 VVIPDQQVVFRLPEMIRGGSPV-VYDEKKKSRFGILNKNAKDASSIQWIEVPDCFCFHLW 475

Query: 347 NAWE--EGDEVVLITCRLENPDLDMVSGSVKEKLENFANELYEMRFNLKSGLASQKKLSE 406
           N+WE  E DEVV+I   +  PD         E  E   + L E+R NLK+G ++++ +  
Sbjct: 476 NSWEEPETDEVVVIGSCMTPPD-----SIFNEHDETLQSVLSEIRLNLKTGESTRRPVIS 535

Query: 407 SAVDFP--RVNEWYTGRYFNSLIFLV-----------------------------FGKEY 466
             V+     VN    GR        +                             +G E 
Sbjct: 536 EQVNLEAGMVNRNLLGRKTRYAYLALTEPWPKVSGFAKVDLSTGEIRKYIYGEGKYGGEP 595

Query: 467 LLKSMGTYEETSNFGVELLFKADSRKSVVNVIDAKTMSSEPVAVVELPHRVPYGFHAFFV 494
           L    G  EE   + +  +   +  KS + +I+A  M  E  A V LP RVPYGFH  F+
Sbjct: 596 LFLPSGDGEEDGGYIMVFVHDEEKVKSELQLINAVNMKLE--ATVTLPSRVPYGFHGTFI 648

BLAST of Spg014656 vs. TAIR 10
Match: AT4G19170.1 (nine-cis-epoxycarotenoid dioxygenase 4 )

HSP 1 Score: 280.0 bits (715), Expect = 4.1e-75
Identity = 172/495 (34.75%), Postives = 255/495 (51.52%), Query Frame = 0

Query: 47  SSKPQHYLSGNFAPVREETPPITDLPVKGYLPECLNGEFVRVGPNPKFSPVAGYHWFDGD 106
           S  P+H LS NFAPV +E PP     + G LP  LNG ++R GPNP+F P   YH FDGD
Sbjct: 104 SVDPKHVLSDNFAPVLDELPPTDCEIIHGTLPLSLNGAYIRNGPNPQFLPRGPYHLFDGD 163

Query: 107 GMIHGLRIKDGKATYVSRYVKTSRLKQEEHFGGAKFMKIGDLKGFFGLIMVNMQMLRAKL 166
           GM+H ++I +GKAT  SRYVKT +   E+  G      +    GF G+     +      
Sbjct: 164 GMLHAIKIHNGKATLCSRYVKTYKYNVEKQTGAPVMPNV--FSGFNGVTASVARGALTAA 223

Query: 167 KVLDVSY----GTGTSNTALIYHHGKLLALSEADKPYVIKVLEDGDLQTLGLLDYDKRLT 226
           +VL   Y    G G +NT+L +   +L AL E+D PY +++ E GD++T+G  D+D +L 
Sbjct: 224 RVLTGQYNPVNGIGLANTSLAFFSNRLFALGESDLPYAVRLTESGDIETIGRYDFDGKLA 283

Query: 227 HSFTAHPKVDPVTGEMFTFGYSHSPPYVTYRVISKDGLMHDPVPI-TIPNPIMMHDFAIT 286
            S TAHPK DP+TGE F F Y   PP++TY      G     VPI ++ +P  +HDFAIT
Sbjct: 284 MSMTAHPKTDPITGETFAFRYGPVPPFLTYFRFDSAGKKQRDVPIFSMTSPSFLHDFAIT 343

Query: 287 ENYAIFMDLPLYFKPK--EMVKENKLIFTFDATKRARFGVLPRYARDDLLMRWFELPNCF 346
           + +AIF ++ L  +    ++V E       D  K  R GV+P+YA D+  M+WFE+P   
Sbjct: 344 KRHAIFAEIQLGMRMNMLDLVLEGGSPVGTDNGKTPRLGVIPKYAGDESEMKWFEVPGFN 403

Query: 347 IFHNANAWEE--GDEVVLITCRLENPDLDMVSGSVKEKLENFANELYEMRFNLKSGLASQ 406
           I H  NAW+E  G+ VVLI      P++  +  ++ E+++     + +++ +L +G+  +
Sbjct: 404 IIHAINAWDEDDGNSVVLIA-----PNIMSIEHTL-ERMDLVHALVEKVKIDLVTGIVRR 463

Query: 407 KKLSESAVDFPRVNEWYTGRYFNSLIFLV------------------------------- 466
             +S   +DF  +N  + GR    +   +                               
Sbjct: 464 HPISARNLDFAVINPAFLGRCSRYVYAAIGDPMPKISGVVKLDVSKGDRDDCTVARRMYG 523

Query: 467 ---FGKE--YLLKSMGTYEETSNFGVELLFKAD--SRKSVVNVIDAKTMSSEPVAVVELP 495
              +G E  ++ +  G  E   + G  + +  D  + +S   V+DAK+   E VA V LP
Sbjct: 524 SGCYGGEPFFVARDPGNPEAEEDDGYVVTYVHDEVTGESKFLVMDAKSPELEIVAAVRLP 583

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022148582.11.3e-25382.71carotenoid 9,10(9',10')-cleavage dioxygenase 1 [Momordica charantia][more]
AFU91489.13.0e-25382.53carotenoid cleavage dioxygenase 1 [Momordica charantia][more]
KAG6575691.11.9e-25282.56Carotenoid 9,10(9',10')-cleavage dioxygenase 1, partial [Cucurbita argyrosperma ... [more]
XP_022953563.14.3e-25282.37carotenoid 9,10(9',10')-cleavage dioxygenase 1 isoform X1 [Cucurbita moschata][more]
XP_022991870.14.3e-25282.37carotenoid 9,10(9',10')-cleavage dioxygenase 1 isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
O655724.3e-22371.46Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Arabidopsis thaliana OX=3702 G... [more]
Q84KG53.4e-22071.89Carotenoid 9,10(9',10')-cleavage dioxygenase OS=Crocus sativus OX=82528 GN=CCD P... [more]
Q8LP175.7e-21570.69Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Pisum sativum OX=3888 GN=CCD1 ... [more]
Q94IR27.0e-21369.92Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Phaseolus vulgaris OX=3885 GN=... [more]
C3VEQ41.1e-20567.48Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Oncidium hybrid cultivar OX=14... [more]
Match NameE-valueIdentityDescription
A0A6J1D5G16.5e-25482.71carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Momordica charantia OX=3673 GN... [more]
K4K0F31.4e-25382.53Carotenoid cleavage dioxygenase 1 OS=Momordica charantia OX=3673 GN=CCD1 PE=2 SV... [more]
A0A6J1JU532.1e-25282.37carotenoid 9,10(9',10')-cleavage dioxygenase 1 isoform X1 OS=Cucurbita maxima OX... [more]
A0A6J1GNN02.1e-25282.37carotenoid 9,10(9',10')-cleavage dioxygenase 1 isoform X1 OS=Cucurbita moschata ... [more]
A0A1S3CFM34.3e-25081.63carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Cucumis melo OX=3656 GN=LOC103... [more]
Match NameE-valueIdentityDescription
AT3G63520.13.1e-22471.46carotenoid cleavage dioxygenase 1 [more]
AT3G14440.12.5e-8036.02nine-cis-epoxycarotenoid dioxygenase 3 [more]
AT1G30100.11.6e-7934.76nine-cis-epoxycarotenoid dioxygenase 5 [more]
AT1G78390.13.0e-7835.48nine-cis-epoxycarotenoid dioxygenase 9 [more]
AT4G19170.14.1e-7534.75nine-cis-epoxycarotenoid dioxygenase 4 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004294Carotenoid oxygenasePFAMPF03055RPE65coord: 451..491
e-value: 2.9E-8
score: 33.2
coord: 58..417
e-value: 1.2E-94
score: 318.1
IPR004294Carotenoid oxygenasePANTHERPTHR10543BETA-CAROTENE DIOXYGENASEcoord: 439..495
coord: 13..418
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..24
NoneNo IPR availablePANTHERPTHR10543:SF124CAROTENOID CLEAVAGE DIOXYGENASE 1-3coord: 439..495
NoneNo IPR availablePANTHERPTHR10543:SF124CAROTENOID CLEAVAGE DIOXYGENASE 1-3coord: 13..418

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg014656.1Spg014656.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016121 carotene catabolic process
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0010436 carotenoid dioxygenase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen