Spg014527 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg014527
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Alpha/beta-Hydrolases superfamily protein
Location	scaffold3: 38709846 .. 38715605 (+)
RNA-Seq Expression	Spg014527
Synteny	Spg014527

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAATTTGAAAAGGTTTAGTTCGTTGAGGTATAGTTTAGGATTTTTGATAAGCGGAACTTTAGGAAAAATTCATTTTTTTGATATGTTTCAAAACCGTCCCGCTTTTGGTCATTCGGTCAAAATTCTCTAATATTTTTTCTAAATTAATTTTCAATTTGACAAAACTTGAAAATTTAAACCCAAATCTTTGGCTCATTTTCTAGTAATAAATAATCACCTCTAACAATTCCGGCTTCATAAATTCCCGGCTGGTGCAAAGAAAAAAAGAAAAAGAAAAAAGAAAAAAGAAAATAGCTCAGCTCAATTTACATAAAACTCACAATAGTGATCTAAAATGTGGTTTAAATCTCCCTTCAGAAGTCGTCGGAAAAAGAAAAAGAAAAAGAAAAGAAAAAAAAAAATTCCGGCCTCACTCTCCTGCCCTGCGTAATGAAGGTCCCGGCTCGTTAAGAAAAACCGCCAAAGATCTGCCGCAATCCCTCTCTCGAGCGTCAAATTCTTGCCGACGAAATTCTCCACCGAAATCCCTCCGCCGCCGTCATGGCCAGTCGAACTGCCAGGCGCCTTCTTCACAACTTCCGTAAGTGAAATTTTCTTTTACTTCTTTGTTCAATTGTTACTGCCTCATGAATGTCTGAGAACTTGTATCTGCTTCTTGATTGTGGAATCAGTGCGAGTTCAATGTCAGTTTGATGTCGTTTGCGGTTTTCTTTATGTGTTTCTTGGAGAATCTTGATACTCTATGAAAATGTGATGATGAATCGTGCGTTCTTTCTGTTACGCATTCTATATATGCTGCATTGAGAAGAAAAGTCGAGCTTGAGTTTCGTAACTTTGTTTTGTTCAATCTTTTAGTTAGTTTGGGATTGATAAGCTGTTGAAGTTGTTTGTGGAAGGCAATGTGATTTGCTTAATCTAATTCATTATCTTATCTGATGTCGAATTCGATTGGGTTCTTTAATTTTTAAGTTTTTCCAAACAGGGAAAATGGAAGTAGATATGAATTGAATTATAAATCTATAATTGATATAATTCAGTCTTAAATGGATTTGAATGCGAATGAACCTTCAGCTATTTTTGTATCTTGAGTAGGTTTGTTCCGTGCATAACTGTTAATTAACACGGAGAGTTGGTTAGTATGCCTAACTTGTAAAGGGTATTATTCTGATTTGGTCAATACTATTCAACTACCTAATTGCGAGGGTTTAGGGTTTCGGGTTTTAGGGGTTTTAGTTCTTATAGTTTACATTGATGAATGATTCTGGTCACACTTCTATCTTTAATCGTAAGTTATAAAATGGCTTTCTCGGGTATCAATTTAAGGTTTCATGGCTATAATGTAGATGTTTTTTCATATGGGGTATGATATTTACATGGATTGTTTTTTCTTCAACCCTGCAGATTATGCCAGAAGCTTTCTCAGGAAACAGGAGTGGCAGGCTAGCTCTGTAACTGTGAATAGTGTTCTTTTGCATTTTCAGAGGTCAAAAGCAGATAAGGTGGGACAAGGTGTGCATAAAGAAGGTGAAGTTTTACCTGGAGTTTCTGTAAGGTCGGAAATGGAGAATACTAGCAAAAAAGGTTCTAAACCTACGAAGTACTTTTCTGATGCTGAAGATATAAGTGATAGTAACCGGAGGTTAGACCTTGCATGGCTGACAGTGACAAAAGCTCTTGAACCAGCTTTGCAATTGTTTAGGTGGGCACTGTCATCAGGTCTGTTTATGTTAGTAGGATTTCTCTTCTCTCTTGTGGGCAATCATTATTTCTTTACTTTGATCATTACTTGCTTTCGGGAAAAAATGTATAGATTAAATATTTTATTGCTTAACGAACACAATTTACAGTCTTCTAATTTTCTGTAGATGTACTTCTGTTTGTTTAAGACTGATGGTGGCCTATACTGTCTGCATTTTATTTTTGCTTTTGTCATGTTCTTTTTAAAGTTGCAGGCTGTGTCTAAGTTATAGTCTTGGAGGCTAATTAAGTTTTTGTGACTTGCAGGGGATGAAATTCAGAATCAAATTCCACCTAGAAGTCGTTCTGTTTCAGAAATCATTGCAAGTATCCAGCGGAGTAAGATGGGAATCCAGGATTGGAGCTTGAGTGATCTTACAATTGGATTATGTCTTATATATCTCCGTCAAGCTTCAACAAATCCATTGGAGGACGTTAAGGGTGTCCAGATCTCTTCAGATGCAATTGTAATCTGTTCGCATCTTTTTTATTTTCTTATAAAATATATGGACTTATTGAAATTATGTTTTCTTTCCTTTAAGAAGTTTCAAACCCTTATAAGCCTGAATTGAAGATCTTATATAGGTAATGATTTTGTGAATCAGGTCGAAGACCTCATCTACTACCTTGAACTAGCTGATGGTTCTTATAAGGACTCTGCAGCTATGCTGGCAAGAAGAACCATGCTTCGAGAGTGCAACATATTGAAATTTGTTAAAGATTCCAGTGTTATGAGGCCTGGTTATTATATAGGGGTTGATACTAGAAAGAAACTTGTAATCTTTGGAATTCGAGGAACTCGCACAGTATATGATCTTATCACTGATATTATTACATCAAGCGACAGAGAAGAAGTGACATTTGAAGGATATTCTACGCACTTTGGCACTTCTGAATCTGCTCGCTGGTTTCTTGATCATGAGATCGGAATGATAAGAAGATGCTTGGAGAAATATCAGGTGGTATTTATGTTTACTAAAGTTCTTTTAGCTTATTCTTTATGTTCCTCCATTGCCCTGTTACCATTGTTGTGGCTGTATCTAATTCATTAACAGGGTTTTAGGTTAAGGCTGGTGGGTCATTCTCTTGGTGGAGCTACAGCTTCGTTACTAGCTGTAATGCTTCGTAAAATGTCAAATAAGGAGCTTGGATTTAGCCCTGATATTGTGTCTGCCATTGGATTTGCAACACCACCTTGCGTTTCAAGGAAACTTGCTGAGAGTTGTGCTGACTATGTTACCACCGTAGTGATGCAGGTTTGCCACATGCAGTCCTCATATGTATTCTAAGCAAAAGCTACAATTTTTTAAAACACAATTTCCTGGAGTTTTATTTCGGTAGTTCTTGTTTCATTCTGTTGCTTTACTTAAAGTCTCTGACTTTACTTTGGGCAGGATGATGTTATCCCTAGATTAAGTGTAGCTTCTCTAACCAGGCTAAGGATTGAAATTCTACAAACTGATTGGTAAGTTATTATGCTTTATTTGAAAGGCTAAAGGTAATTTTCTTCTATGGATGTGCTGAAAATAAATATGGCTGTTGAAGGTGTGAGGAGACATGTTGAAAGCATTTTTAATGATTATGATATGATAAACCGGGATTCAAAACTGTCCCTGTAAATCTAAGGCTCTAAGTTAAGCGATCACCTAATCCTCTTGAAAATAATTTTTATCTTTTATAATGTGATGACCACAATTTATATTCTGCATCTGAATCTTGGTTACAGTCTTATCACAATATCTGTTATGTGTTATCTAAAGCATGGGTCTTAAAAATCAAGCTTATAAATACTACTTCCACTCCACCTATGGATTTCTTTGTTTTGTTATCCACTTTTTACCTATGTTTTCAAAATTTAAGCCAAGTTTTGAAAACTAGAAAAAGTAAAAACTACTTTTTTTGTTTTTGTTTTTGTTTTTGTTTTGTTTTGTTTTGTTTTGTTTTTTTTTTTTTTTTTTTGTTTTTTTTTTGTTTTTTTTTTTTTGTATTTGGCTAAGAATTCAAAATTTTTATTGAGAGAGATGAAAAATATTGGGAAAAAATGGTTGAGAAAACAAGCATAATCTTTAAAAACCAAAAACCAAAAATAAAAAACCAAATGGTTATCAAATGGAGCCCAAGTAGTATATGCTAAGTATTACAACTGATAAATCTAGTTTAATTAGGAATTGGATTGAGAAATGTGATTTCAATTTCCTTATCATAGTTATCTTATGGAATTTTGTATTAATTGTATAGTGGACTGGTAGAATTCACTTAGATTCAAAAGATATTGAGATGCACTTAGGAAAGTTCGTGAGTCCCTTAAGTTTCACCTATTACTTAGTTACTTCTAGTCAAGTATGGACTGAATAGACATCGCCATGAAAGTTTGGTAACCAAAACTTAAAATTTAACTATAATATAAATATGTTGAATATATCAACCTTGTGCAGCTGTTAAACTTGTGATTTCGGAGTTTGCAAATTCAATTGCGGAACTTCTATTTACTATTCAGACATATGGTCAATGCTGCTTTTTAAAACTTCCATTCAATTAATTGCTCTGAGGATGACTAGATTTTGAAAATGTTACACATACCGTCAGGCATCAATCTAATTTGGCACGAGCTATTTTAAGGGATAAAAAAAGAAAATATTATGGAATTTAATTAAAGAGAGGAAATTAACATGCTGATTTCTTGTTATTGAACAAGCTAGTGGAGAAGGCAATCTCAAATTTCATGTTCCATCTGTTTCAGGATGAGTGTGATTGAAAAACAAGACTTGAAAGGTATCGTTGGTTTGGTCACAAATGCAAAGCAGGTGGTTACATCAGTTCAAGACGTTGCTCGAAAACTTGCAGATTTTGCAAAGTTCACAAGCAAGAAAAGATCCTCTGGTAAGCCAATCCTAATGTTCACAGGTTTACTTCACATTTTCCTCAGGCATAAATTAATTGAATATTATTGTTTTAATGGCTTTCAGATAGTTTTGGTCCTCGATTGGGTAAGATTAACCGAGCAGACTGAGCAGTCATGTGATGAAATTTTGTATTCATAATATTTTCTCCTTGACCGAATCTGCATCAAATTGATCAACTAACTTCTAGACTCTAAACAAGACTGTTCTTTTACCGTACGTTTTTATTTTTTATTTGCTATGTTTTTTCTCAAGCATATATTTACTAGCAAATGTAAATGTTCCTTGGTCAGAATTTATCTATTTCATGTCAATTTTACAGTTTTCGACTAAAATCCAATAAAAGATTAGGGACGATTTAGCCAATGTTTTCCAACAGGGAACTCTTCACAGAAGTATTTCGTTTTGATTTAATTTCAAGGAAATGTAGTAAAATCACACCTCCTCCACACTGCCCTATTTCTAGTTTCTACCTTCGCCTTAGCCACTCGCTTTCTTCCCTTTTTAACAGATGATAGCAATAGAAAAGAGTCTCATGTGGCTTCTGGACCTCCTCCTTCACACCCTACCGCTGCGCTGCAGAGTGTTGCCGCTGCTGAAAATGAAGCCGCAAAATGTAAAATTCCCGAGGAGCTATTCGTACCGGGAACGGTTTATTATCTGAAGAGGCACATGGACAGTACCCCAGAATACTTCACTCTTTGGAAGCGGCATCCTGATGAACATTTCCAGCAAATTGTGCTATCAAGCAACCTCATTTCAAATCATAAATGTGACAGCCACTACTATGCTTTGAGGGATGTACTTAAAAGCCTGCCATCATGCAACGATGAAGAAGGTATTTTCTCATAAAAGTCAATTTTAGCTCTATTGGGTTAATCACACATATAGCACAAGGGTTCAATACCCCAGTGGAAATAAACTACTCAACATTGTAATACAAAGAGAACAAAAGAGAGATCTCTTAGTGGGATTTTTGTAGTCTCAAGGCTTTTTTTTTTTTCTGTTGGCCATTTAGGGAGTCTTTTTGTTAACTCCATTGGTCGGTGTACATACTTTTCACTCCCTTTAATGGGAATTTTTGTTTCTGATGAGAAAAAAAAAATACAATTAGAGTTCCCTC

mRNA sequence

ATGGCCAGTCGAACTGCCAGGCGCCTTCTTCACAACTTCCATTATGCCAGAAGCTTTCTCAGGAAACAGGAGTGGCAGGCTAGCTCTGTAACTGTGAATAGTGTTCTTTTGCATTTTCAGAGGTCAAAAGCAGATAAGGTGGGACAAGGTGTGCATAAAGAAGGTGAAGTTTTACCTGGAGTTTCTGTAAGGTCGGAAATGGAGAATACTAGCAAAAAAGGTTCTAAACCTACGAAGTACTTTTCTGATGCTGAAGATATAAGTGATAGTAACCGGAGGTTAGACCTTGCATGGCTGACAGTGACAAAAGCTCTTGAACCAGCTTTGCAATTGTTTAGGTGGGCACTGTCATCAGGGGATGAAATTCAGAATCAAATTCCACCTAGAAGTCGTTCTGTTTCAGAAATCATTGCAAGTATCCAGCGGAGTAAGATGGGAATCCAGGATTGGAGCTTGAGTGATCTTACAATTGGATTATGTCTTATATATCTCCGTCAAGCTTCAACAAATCCATTGGAGGACGTTAAGGGTGTCCAGATCTCTTCAGATGCAATTGTCGAAGACCTCATCTACTACCTTGAACTAGCTGATGGTTCTTATAAGGACTCTGCAGCTATGCTGGCAAGAAGAACCATGCTTCGAGAGTGCAACATATTGAAATTTGTTAAAGATTCCAGTGTTATGAGGCCTGGTTATTATATAGGGGTTGATACTAGAAAGAAACTTGTAATCTTTGGAATTCGAGGAACTCGCACAGTATATGATCTTATCACTGATATTATTACATCAAGCGACAGAGAAGAAGTGACATTTGAAGGATATTCTACGCACTTTGGCACTTCTGAATCTGCTCGCTGGTTTCTTGATCATGAGATCGGAATGATAAGAAGATGCTTGGAGAAATATCAGGTGGGTTTTAGGTTAAGGCTGGTGGGTCATTCTCTTGGTGGAGCTACAGCTTCGTTACTAGCTGTAATGCTTCGTAAAATGTCAAATAAGGAGCTTGGATTTAGCCCTGATATTGTGTCTGCCATTGGATTTGCAACACCACCTTGCGTTTCAAGGAAACTTGCTGAGAGTTGTGCTGACTATGTTACCACCGTAGTGATGCAGGATGATGTTATCCCTAGATTAAGTGTAGCTTCTCTAACCAGGCTAAGGATTGAAATTCTACAAACTGATTGGATGAGTGTGATTGAAAAACAAGACTTGAAAGGTATCGTTGGTTTGGTCACAAATGCAAAGCAGGTGGTTACATCAGTTCAAGACGTTGCTCGAAAACTTGCAGATTTTGCAAAGTTCACAAGCAAGAAAAGATCCTCTGGTAAGCCAATCCTAATGTTCACAGATGATAGCAATAGAAAAGAGTCTCATGTGGCTTCTGGACCTCCTCCTTCACACCCTACCGCTGCGCTGCAGAGTGTTGCCGCTGCTGAAAATGAAGCCGCAAAATGTAAAATTCCCGAGGAGCTATTCGTACCGGGAACGGTTTATTATCTGAAGAGGCACATGGACAGTACCCCAGAATACTTCACTCTTTGGAAGCGGCATCCTGATGAACATTTCCAGCAAATTGTGCTATCAAGCAACCTCATTTCAAATCATAAATGTGACAGCCACTACTATGCTTTGAGGGATGTACTTAAAAGCCTGCCATCATGCAACGATGAAGAAGGTATTTTCTCATAA

Coding sequence (CDS)

ATGGCCAGTCGAACTGCCAGGCGCCTTCTTCACAACTTCCATTATGCCAGAAGCTTTCTCAGGAAACAGGAGTGGCAGGCTAGCTCTGTAACTGTGAATAGTGTTCTTTTGCATTTTCAGAGGTCAAAAGCAGATAAGGTGGGACAAGGTGTGCATAAAGAAGGTGAAGTTTTACCTGGAGTTTCTGTAAGGTCGGAAATGGAGAATACTAGCAAAAAAGGTTCTAAACCTACGAAGTACTTTTCTGATGCTGAAGATATAAGTGATAGTAACCGGAGGTTAGACCTTGCATGGCTGACAGTGACAAAAGCTCTTGAACCAGCTTTGCAATTGTTTAGGTGGGCACTGTCATCAGGGGATGAAATTCAGAATCAAATTCCACCTAGAAGTCGTTCTGTTTCAGAAATCATTGCAAGTATCCAGCGGAGTAAGATGGGAATCCAGGATTGGAGCTTGAGTGATCTTACAATTGGATTATGTCTTATATATCTCCGTCAAGCTTCAACAAATCCATTGGAGGACGTTAAGGGTGTCCAGATCTCTTCAGATGCAATTGTCGAAGACCTCATCTACTACCTTGAACTAGCTGATGGTTCTTATAAGGACTCTGCAGCTATGCTGGCAAGAAGAACCATGCTTCGAGAGTGCAACATATTGAAATTTGTTAAAGATTCCAGTGTTATGAGGCCTGGTTATTATATAGGGGTTGATACTAGAAAGAAACTTGTAATCTTTGGAATTCGAGGAACTCGCACAGTATATGATCTTATCACTGATATTATTACATCAAGCGACAGAGAAGAAGTGACATTTGAAGGATATTCTACGCACTTTGGCACTTCTGAATCTGCTCGCTGGTTTCTTGATCATGAGATCGGAATGATAAGAAGATGCTTGGAGAAATATCAGGTGGGTTTTAGGTTAAGGCTGGTGGGTCATTCTCTTGGTGGAGCTACAGCTTCGTTACTAGCTGTAATGCTTCGTAAAATGTCAAATAAGGAGCTTGGATTTAGCCCTGATATTGTGTCTGCCATTGGATTTGCAACACCACCTTGCGTTTCAAGGAAACTTGCTGAGAGTTGTGCTGACTATGTTACCACCGTAGTGATGCAGGATGATGTTATCCCTAGATTAAGTGTAGCTTCTCTAACCAGGCTAAGGATTGAAATTCTACAAACTGATTGGATGAGTGTGATTGAAAAACAAGACTTGAAAGGTATCGTTGGTTTGGTCACAAATGCAAAGCAGGTGGTTACATCAGTTCAAGACGTTGCTCGAAAACTTGCAGATTTTGCAAAGTTCACAAGCAAGAAAAGATCCTCTGGTAAGCCAATCCTAATGTTCACAGATGATAGCAATAGAAAAGAGTCTCATGTGGCTTCTGGACCTCCTCCTTCACACCCTACCGCTGCGCTGCAGAGTGTTGCCGCTGCTGAAAATGAAGCCGCAAAATGTAAAATTCCCGAGGAGCTATTCGTACCGGGAACGGTTTATTATCTGAAGAGGCACATGGACAGTACCCCAGAATACTTCACTCTTTGGAAGCGGCATCCTGATGAACATTTCCAGCAAATTGTGCTATCAAGCAACCTCATTTCAAATCATAAATGTGACAGCCACTACTATGCTTTGAGGGATGTACTTAAAAGCCTGCCATCATGCAACGATGAAGAAGGTATTTTCTCATAA

Protein sequence

MASRTARRLLHNFHYARSFLRKQEWQASSVTVNSVLLHFQRSKADKVGQGVHKEGEVLPGVSVRSEMENTSKKGSKPTKYFSDAEDISDSNRRLDLAWLTVTKALEPALQLFRWALSSGDEIQNQIPPRSRSVSEIIASIQRSKMGIQDWSLSDLTIGLCLIYLRQASTNPLEDVKGVQISSDAIVEDLIYYLELADGSYKDSAAMLARRTMLRECNILKFVKDSSVMRPGYYIGVDTRKKLVIFGIRGTRTVYDLITDIITSSDREEVTFEGYSTHFGTSESARWFLDHEIGMIRRCLEKYQVGFRLRLVGHSLGGATASLLAVMLRKMSNKELGFSPDIVSAIGFATPPCVSRKLAESCADYVTTVVMQDDVIPRLSVASLTRLRIEILQTDWMSVIEKQDLKGIVGLVTNAKQVVTSVQDVARKLADFAKFTSKKRSSGKPILMFTDDSNRKESHVASGPPPSHPTAALQSVAAAENEAAKCKIPEELFVPGTVYYLKRHMDSTPEYFTLWKRHPDEHFQQIVLSSNLISNHKCDSHYYALRDVLKSLPSCNDEEGIFS

Homology

[Close]

BLAST of Spg014527 vs. NCBI nr
Match: XP_038897252.1 (uncharacterized protein LOC120085373 isoform X1 [Benincasa hispida])

HSP 1 Score: 885.9 bits (2288), Expect = 1.8e-253
Identity = 464/557 (83.30%), Postives = 501/557 (89.95%), Query Frame = 0


Query: 1   MASRTARRLLHNFHYARSFLRKQEWQASSVTVNSVLLHFQRSKADKVGQGVHKEGEVLPG 60
           MA+ TARRLLHNFHYAR+FLRKQ+WQA SVTVN+VL+HFQR+K DKV +GVHKEGEVL  
Sbjct: 1   MANGTARRLLHNFHYARNFLRKQQWQARSVTVNNVLMHFQRTKVDKVEKGVHKEGEVLQE 60

Query: 61  VSVRSEMENTSKKGSKPTKYFSDAEDISDSNRRLDLAWLTVTKALEPALQLFRWALSSGD 120
           VS RSEM   SKKGSK TK  S+AED +DSNR L+LAW   TKA+EPALQL+RWALSSGD
Sbjct: 61  VSGRSEMGKNSKKGSKRTKSVSEAEDTTDSNRMLELAWW--TKAIEPALQLYRWALSSGD 120

Query: 121 EIQNQIPPRSRSVSEIIASIQRSKMGIQDWSLSDLTIGLCLIYLRQASTNPLEDVKGVQI 180
                IPPR+RSVSEIIASIQRSK GIQDWSLSDLTIGLCLIYLRQASTNPLED+KGVQI
Sbjct: 121 ----GIPPRTRSVSEIIASIQRSKTGIQDWSLSDLTIGLCLIYLRQASTNPLEDLKGVQI 180

Query: 181 SSDAIVEDLIYYLELADGSYKDSAAMLARRTMLRECNILKFVKDSSVMRPGYYIGVDTRK 240
           SSDA+VEDLIY++ELA GSYK+SAAMLA+ TMLRECNILKFVK+SSVMRPGYYIGVDTRK
Sbjct: 181 SSDAMVEDLIYFVELAKGSYKNSAAMLAKTTMLRECNILKFVKNSSVMRPGYYIGVDTRK 240

Query: 241 KLVIFGIRGTRTVYDLITDIITSSDREEVTFEGYSTHFGTSESARWFLDHEIGMIRRCLE 300
           KLVIFGIRGT TVYDLITDIIT+SD  +VTFEGYS HFGTSESARWFL HEIGMIRRCLE
Sbjct: 241 KLVIFGIRGTYTVYDLITDIITTSD-GDVTFEGYSMHFGTSESARWFLQHEIGMIRRCLE 300

Query: 301 KYQVGFRLRLVGHSLGGATASLLAVMLRKMSNKELGFSPDIVSAIGFATPPCVSRKLAES 360
           KYQ GFRL+LVGHSLGGA ASLLAVMLRKMSNKELGFSPDIVSAIG+ATPPCVSRKLAES
Sbjct: 301 KYQ-GFRLKLVGHSLGGAIASLLAVMLRKMSNKELGFSPDIVSAIGYATPPCVSRKLAES 360

Query: 361 CADYVTTVVMQDDVIPRLSVASLTRLRIEILQTDWMSVIEKQDLKGIVGLVTNAKQVVTS 420
           CADYVTTVVMQ DVIPRLSVASLTRLRIEILQTDWMS+I+K+D K ++GLVTNAKQVVTS
Sbjct: 361 CADYVTTVVMQGDVIPRLSVASLTRLRIEILQTDWMSLIDKEDWKSVIGLVTNAKQVVTS 420

Query: 421 VQDVARKLADFAKFTSKKRSSGKPILMFTDDSNRKESHVASGPPPSHPTAALQSVAAAEN 480
           VQDVARKLADFAKF SKKRSS        DDSNRK+S VASG PP HPTAALQS  A +N
Sbjct: 421 VQDVARKLADFAKFPSKKRSS--------DDSNRKKSDVASGSPP-HPTAALQSATATQN 480

Query: 481 EAAKCKIPEELFVPGTVYYLKRHMDSTPEYFTLWKRHPDEHFQQIVLSSNLISNHKCDSH 540
           EAA+CKIP+ELFVPGTVYYLKRH DSTPEYFTLWKR+PDEHFQQIVLSS LIS+H CDSH
Sbjct: 481 EAARCKIPDELFVPGTVYYLKRHTDSTPEYFTLWKRYPDEHFQQIVLSSILISDHTCDSH 540

Query: 541 YYALRDVLKSLPSCNDE 558
           YYALRDVLK LPSC+ +
Sbjct: 541 YYALRDVLKGLPSCSQQ 540

[Close]

BLAST of Spg014527 vs. NCBI nr
Match: XP_023513693.1 (sn1-specific diacylglycerol lipase beta [Cucurbita pepo subsp. pepo])

HSP 1 Score: 881.7 bits (2277), Expect = 3.3e-252
Identity = 467/564 (82.80%), Postives = 498/564 (88.30%), Query Frame = 0


Query: 1   MASRTARRLLHNFHYARSFLRKQEWQASSVTVNSVLLHFQRSKADKVGQGVHKEGEVLPG 60
           M++ TARRLLHN + ARS LRKQ+WQASSVTVNSVL+H QRSKAD V   V KE EVLP 
Sbjct: 1   MSNGTARRLLHNLYSARSSLRKQKWQASSVTVNSVLMHLQRSKADMVENNVRKENEVLPE 60

Query: 61  VSVRSEMENTSKKGSKPTKYFSDAEDISDSNRRLDLAWLTVTKALEPALQLFRWALSSGD 120
           VSVRSEME  SK+GSK  KYFSDAEDISDSNRRL+LAWL  TKAL+ A QL+RWALSSGD
Sbjct: 61  VSVRSEMEKNSKEGSKTPKYFSDAEDISDSNRRLELAWL--TKALKSASQLYRWALSSGD 120

Query: 121 EIQNQIPPRSRSVSEIIASIQRSKMGIQDWSLSDLTIGLCLIYLRQASTNPLEDVKGVQI 180
            IQNQI PRSRSVSEIIASIQRSKMGIQ+WSLSDLTIGL LIYLRQASTNPL D+KGVQI
Sbjct: 121 GIQNQIQPRSRSVSEIIASIQRSKMGIQEWSLSDLTIGLFLIYLRQASTNPLADIKGVQI 180

Query: 181 SSDAIVEDLIYYLELADGSYKDSAAMLARRTMLRECNILKFVKDSSVMRPGYYIGVDTRK 240
           +S+AIVEDLIYYLELA G YKDSAAMLAR TMLRE NILKF+KDSSVMRPGYYIGVDTRK
Sbjct: 181 TSNAIVEDLIYYLELAKGCYKDSAAMLARNTMLREPNILKFIKDSSVMRPGYYIGVDTRK 240

Query: 241 KLVIFGIRGTRTVYDLITDIITSSDREEVTFEGYSTHFGTSESARWFLDHEIGMIRRCLE 300
           KLVIFGIRGT +VYDLITDI TSSD EEVT EGYSTHFGTSESARWFLDHEI MIRRCL+
Sbjct: 241 KLVIFGIRGTHSVYDLITDITTSSD-EEVTLEGYSTHFGTSESARWFLDHEIEMIRRCLK 300

Query: 301 KYQVGFRLRLVGHSLGGATASLLAVMLRKMSNKELGFSPDIVSAIGFATPPCVSRKLAES 360
           KYQ GFRLRLVGHSLGGA ASLLAVMLRK SNKELGFSPDIVSAIGFATPPCVSRKLAES
Sbjct: 301 KYQ-GFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFSPDIVSAIGFATPPCVSRKLAES 360

Query: 361 CADYVTTVVMQDDVIPRLSVASLTRLRIEILQTDWMSVIEKQDLKGIVGLVTNAKQVVTS 420
           CADYVTTVVMQDD+IPRLS+ASLT+LR+EILQTDW SVIE+QD K I GL+TNAKQVVTS
Sbjct: 361 CADYVTTVVMQDDIIPRLSLASLTKLRVEILQTDWTSVIEEQDWKNIYGLLTNAKQVVTS 420

Query: 421 VQDVARKLADFAKFTSKKRSSGKPILMFTDDSNRKESHVASGPPPSHPTAALQSVAAAEN 480
           VQDVARKL D+AKFTSKK  S        DDS  K SHVASGPPPSHPTA +Q    A+N
Sbjct: 421 VQDVARKLGDYAKFTSKKGPS--------DDSYGKGSHVASGPPPSHPTAVVQRADTAQN 480

Query: 481 EAAKCKIPEELFVPGTVYYLKRH--MDSTPEYFTLWKRHPDEHFQQIVLSSNLISNHKCD 540
           EAA+CKIPEELFVPGTVYYLK+H  ++   EYFTLWKRHPDEHFQQIVLSSNLIS+HKCD
Sbjct: 481 EAARCKIPEELFVPGTVYYLKKHVNLEEAYEYFTLWKRHPDEHFQQIVLSSNLISDHKCD 540

Query: 541 SHYYALRDVLKSLPSCNDEEGIFS 563
           SHY+ALRDVLK LPSCND EGIFS
Sbjct: 541 SHYHALRDVLKGLPSCND-EGIFS 551

[Close]

BLAST of Spg014527 vs. NCBI nr
Match: XP_022959891.1 (sn1-specific diacylglycerol lipase beta [Cucurbita moschata])

HSP 1 Score: 878.2 bits (2268), Expect = 3.7e-251
Identity = 466/564 (82.62%), Postives = 497/564 (88.12%), Query Frame = 0


Query: 1   MASRTARRLLHNFHYARSFLRKQEWQASSVTVNSVLLHFQRSKADKVGQGVHKEGEVLPG 60
           M++ TARRLLHN + ARS LRKQ+WQASSVTVNSVL+H QRSKAD V   V KE EVLP 
Sbjct: 1   MSNGTARRLLHNLYSARSSLRKQKWQASSVTVNSVLMHLQRSKADMVENNVRKENEVLPE 60

Query: 61  VSVRSEMENTSKKGSKPTKYFSDAEDISDSNRRLDLAWLTVTKALEPALQLFRWALSSGD 120
           VSVRSEME  SK+GSK  KYFSDAEDISDSNRRL+LAWL  TKAL+ A QL+RWALSSGD
Sbjct: 61  VSVRSEMEKNSKEGSKTPKYFSDAEDISDSNRRLELAWL--TKALKSASQLYRWALSSGD 120

Query: 121 EIQNQIPPRSRSVSEIIASIQRSKMGIQDWSLSDLTIGLCLIYLRQASTNPLEDVKGVQI 180
            IQNQI PRSRSVSEIIASIQRSKMGIQ+WSLSDLTIGL LIYLRQASTNPL D+KGVQI
Sbjct: 121 GIQNQIQPRSRSVSEIIASIQRSKMGIQEWSLSDLTIGLFLIYLRQASTNPLADIKGVQI 180

Query: 181 SSDAIVEDLIYYLELADGSYKDSAAMLARRTMLRECNILKFVKDSSVMRPGYYIGVDTRK 240
           +S+AIVEDLIYYLELA G YKDSAAMLAR TMLRE NILKF+KDSSVMRPGYYIGVDTRK
Sbjct: 181 TSNAIVEDLIYYLELAKGCYKDSAAMLARNTMLREPNILKFIKDSSVMRPGYYIGVDTRK 240

Query: 241 KLVIFGIRGTRTVYDLITDIITSSDREEVTFEGYSTHFGTSESARWFLDHEIGMIRRCLE 300
           KLVIFGIRGT +VYDLITDI TSSD EEVT EGYSTHFGTSESARWFLDHEI MIRRCL+
Sbjct: 241 KLVIFGIRGTHSVYDLITDITTSSD-EEVTLEGYSTHFGTSESARWFLDHEIEMIRRCLK 300

Query: 301 KYQVGFRLRLVGHSLGGATASLLAVMLRKMSNKELGFSPDIVSAIGFATPPCVSRKLAES 360
           KYQ GFRLRLVGHSLGGA ASLLAVMLRK SNKELGFSPDIVSAIGFATPPCVSRKLAES
Sbjct: 301 KYQ-GFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFSPDIVSAIGFATPPCVSRKLAES 360

Query: 361 CADYVTTVVMQDDVIPRLSVASLTRLRIEILQTDWMSVIEKQDLKGIVGLVTNAKQVVTS 420
           CADYVTTVVMQDD+IPRLS+ASLT+LR+EILQTDW SVIE+QD K I GL+TNAKQVVTS
Sbjct: 361 CADYVTTVVMQDDIIPRLSLASLTKLRVEILQTDWTSVIEEQDWKNIYGLLTNAKQVVTS 420

Query: 421 VQDVARKLADFAKFTSKKRSSGKPILMFTDDSNRKESHVASGPPPSHPTAALQSVAAAEN 480
           VQDVARKL D+AKFTSKK  S        DDS  K SHVASGPPPS PTA +Q    A+N
Sbjct: 421 VQDVARKLGDYAKFTSKKGPS--------DDSYGKGSHVASGPPPSQPTALVQRADTAQN 480

Query: 481 EAAKCKIPEELFVPGTVYYLKRH--MDSTPEYFTLWKRHPDEHFQQIVLSSNLISNHKCD 540
           EAA+CKIPEELFVPGTVYYLK+H  ++   EYFTLWKRHPDEHFQQIVLSSNLIS+HKCD
Sbjct: 481 EAARCKIPEELFVPGTVYYLKKHVNLEEAYEYFTLWKRHPDEHFQQIVLSSNLISDHKCD 540

Query: 541 SHYYALRDVLKSLPSCNDEEGIFS 563
           SHY+ALRDVLK LPSCND EGIFS
Sbjct: 541 SHYHALRDVLKGLPSCND-EGIFS 551

[Close]

BLAST of Spg014527 vs. NCBI nr
Match: XP_023004816.1 (sn1-specific diacylglycerol lipase beta [Cucurbita maxima])

HSP 1 Score: 875.9 bits (2262), Expect = 1.8e-250
Identity = 465/564 (82.45%), Postives = 496/564 (87.94%), Query Frame = 0


Query: 1   MASRTARRLLHNFHYARSFLRKQEWQASSVTVNSVLLHFQRSKADKVGQGVHKEGEVLPG 60
           M++ TARRLL N + ARS+LRKQ+WQASSVTVNSVL+H QRSKAD V   V KE EVLP 
Sbjct: 1   MSNGTARRLLRNLYSARSYLRKQKWQASSVTVNSVLMHLQRSKADMVENNVRKENEVLPE 60

Query: 61  VSVRSEMENTSKKGSKPTKYFSDAEDISDSNRRLDLAWLTVTKALEPALQLFRWALSSGD 120
           VSVRSEME   KKGSK  KYFSDAEDISDSNRRL+LAWL  TKAL+ A QL+RWALSSGD
Sbjct: 61  VSVRSEMEKNIKKGSKTPKYFSDAEDISDSNRRLELAWL--TKALKSASQLYRWALSSGD 120

Query: 121 EIQNQIPPRSRSVSEIIASIQRSKMGIQDWSLSDLTIGLCLIYLRQASTNPLEDVKGVQI 180
            IQNQI PRSRSVSEIIASIQRSKMGIQ+WSLSDLTIGL LIYLRQASTNPL D+KGVQI
Sbjct: 121 GIQNQIQPRSRSVSEIIASIQRSKMGIQEWSLSDLTIGLFLIYLRQASTNPLADIKGVQI 180

Query: 181 SSDAIVEDLIYYLELADGSYKDSAAMLARRTMLRECNILKFVKDSSVMRPGYYIGVDTRK 240
           +S+AIVEDLIYYLELA G YKDSAAMLAR TMLRE NILKF+KDSSVMRPGYYIGVDTRK
Sbjct: 181 TSNAIVEDLIYYLELAKGCYKDSAAMLARNTMLREPNILKFIKDSSVMRPGYYIGVDTRK 240

Query: 241 KLVIFGIRGTRTVYDLITDIITSSDREEVTFEGYSTHFGTSESARWFLDHEIGMIRRCLE 300
           KLVIFGIRGT +VYDLITDI TSSD EEVTFEGYSTHFGTSESARWFLDHEI MIR+CL+
Sbjct: 241 KLVIFGIRGTHSVYDLITDITTSSD-EEVTFEGYSTHFGTSESARWFLDHEIEMIRQCLK 300

Query: 301 KYQVGFRLRLVGHSLGGATASLLAVMLRKMSNKELGFSPDIVSAIGFATPPCVSRKLAES 360
           KYQ GFRLRLVGHSLGGA ASLLAVMLRK SNKELGF PDIVSAIGFATPPCVSRKLAES
Sbjct: 301 KYQ-GFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFRPDIVSAIGFATPPCVSRKLAES 360

Query: 361 CADYVTTVVMQDDVIPRLSVASLTRLRIEILQTDWMSVIEKQDLKGIVGLVTNAKQVVTS 420
           CADYVTTVVMQDD+IPRLS+ASLT+LRIEILQTDW SVIE+QD K I GL+TNAKQVVTS
Sbjct: 361 CADYVTTVVMQDDIIPRLSLASLTKLRIEILQTDWTSVIEEQDWKNIYGLLTNAKQVVTS 420

Query: 421 VQDVARKLADFAKFTSKKRSSGKPILMFTDDSNRKESHVASGPPPSHPTAALQSVAAAEN 480
           VQDVARKL D+AKFTSKK  S        DDS  K SHVASGPPPS PTA +Q    A+N
Sbjct: 421 VQDVARKLGDYAKFTSKKGPS--------DDSYGKGSHVASGPPPSQPTAVVQRADTAQN 480

Query: 481 EAAKCKIPEELFVPGTVYYLKRH--MDSTPEYFTLWKRHPDEHFQQIVLSSNLISNHKCD 540
           EAA+CKIPEELFVPGTVYYLK+H  ++   EYFTLWKRHPDEHFQQIVLSSNLIS+HKCD
Sbjct: 481 EAARCKIPEELFVPGTVYYLKKHVNLEEAYEYFTLWKRHPDEHFQQIVLSSNLISDHKCD 540

Query: 541 SHYYALRDVLKSLPSCNDEEGIFS 563
           SHY+ALRDVLK LPSCND EGIFS
Sbjct: 541 SHYHALRDVLKGLPSCND-EGIFS 551

[Close]

BLAST of Spg014527 vs. NCBI nr
Match: XP_022156622.1 (uncharacterized protein LOC111023479 [Momordica charantia])

HSP 1 Score: 859.8 bits (2220), Expect = 1.4e-245
Identity = 462/563 (82.06%), Postives = 494/563 (87.74%), Query Frame = 0


Query: 1   MASRTARRLLHNFHYARSFLRKQEWQASSVTVNSVLLHFQRSKADKVGQGVHKEGEVLPG 60
           MAS   RRLLHNFH+ARSFLRKQE QASSVTVNSVL+HF RSKADKV  GVHKEGEVLP 
Sbjct: 1   MASGAVRRLLHNFHHARSFLRKQELQASSVTVNSVLMHFHRSKADKVEIGVHKEGEVLPE 60

Query: 61  VSVRSEMENTSKKGSKPTKYFSDAEDISDSNRRLDLAWLTVTKALEPALQLFRWALS-SG 120
           VSVRSEME  SKKGS+PTKYFSD E+ISDS RR +LAWL  TKALEPALQL+RWALS SG
Sbjct: 61  VSVRSEMEKNSKKGSEPTKYFSDDENISDSKRRSELAWL--TKALEPALQLYRWALSASG 120

Query: 121 DEIQNQIPPRSRSVSEIIASIQRSKMGIQDWSLSDLTIGLCLIYLRQASTNPLEDVKGVQ 180
           D I++Q PPRSRSVSEIIASIQ SKMGIQDWSLSDLTIGL LIYLRQASTNP ED+ GVQ
Sbjct: 121 DGIRDQTPPRSRSVSEIIASIQWSKMGIQDWSLSDLTIGLYLIYLRQASTNPFEDISGVQ 180

Query: 181 ISSDAIVEDLIYYLELADGSYKDSAAMLARRTMLRECNILKFVKDSSVMRPGYYIGVDTR 240
           ISSDAIV+DLIYYLELA GSYKDSAA LAR +MLRECNILKFV DSSVMRPGYYIGVDTR
Sbjct: 181 ISSDAIVQDLIYYLELAKGSYKDSAASLARNSMLRECNILKFVGDSSVMRPGYYIGVDTR 240

Query: 241 KKLVIFGIRGTRTVYDLITDIITSSDREEVTFEGYSTHFGTSESARWFLDHEIGMIRRCL 300
           KKLVIFGIRGT T+ DLITDI+TSSD  EVTFEGY THFGT+ESARWFLDHE+GMIRRCL
Sbjct: 241 KKLVIFGIRGTHTLSDLITDIVTSSD-GEVTFEGYVTHFGTAESARWFLDHEMGMIRRCL 300

Query: 301 EKYQVGFRLRLVGHSLGGATASLLAVMLRKMSNKELGFSPDIVSAIGFATPPCVSRKLAE 360
           EKYQ GFRLRLVGHSLGGA ASLLAVMLRK SNKELGFSPDIVSAIG+ATPPCVSRKLAE
Sbjct: 301 EKYQ-GFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFSPDIVSAIGYATPPCVSRKLAE 360

Query: 361 SCADYVTTVVMQDDVIPRLSVASLTRLRIEILQTDWMSVIEKQDLKGIVGLVTNAKQVVT 420
           SCADYVTTVVMQDD+IPRLS+ASLTRLR EILQTDW SVIEKQD K  +GLVTNA+QVV+
Sbjct: 361 SCADYVTTVVMQDDIIPRLSIASLTRLRNEILQTDWTSVIEKQDWKSFIGLVTNARQVVS 420

Query: 421 SVQDVARKLADFAKFTSKKRSSGKPILMFTDDSNRKESHVASGPPPSHPTAALQSVAAAE 480
           SVQDVARKLAD+AKFTSKK SS        D S RKESHVAS PP  HPTAA ++ AA  
Sbjct: 421 SVQDVARKLADYAKFTSKKISS--------DGSIRKESHVASAPP--HPTAAARNKAA-- 480

Query: 481 NEAAKCKIPEELFVPGTVYYLKRHMDSTPEYFTLWKRHPDEHFQQIVLSSNLISNHKCDS 540
              A+ K PEELFVPGTVYYLKR+ + TPEYFTLWKRHP EHFQ+IVLSSNLIS+H+CDS
Sbjct: 481 --TARYKTPEELFVPGTVYYLKRNTNGTPEYFTLWKRHPGEHFQRIVLSSNLISDHRCDS 540

Query: 541 HYYALRDVLKSLPSCNDEEGIFS 563
           HYYALRDVLK LP+ +D E IFS
Sbjct: 541 HYYALRDVLKGLPARSD-ESIFS 544

[Close]

BLAST of Spg014527 vs. ExPASy Swiss-Prot
Match: P0C1S9 (Diacylglycerol lipase-beta OS=Rattus norvegicus OX=10116 GN=Daglb PE=1 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 5.7e-13
Identity = 56/166 (33.73%), Postives = 93/166 (56.02%), Query Frame = 0


Query: 232 YYIGVDTRKKLVIFGIRGTRTVYDLITDIITSSDREEVTFE--GYSTHFGTSESARWFLD 291
           + + +D RK+ V+  +RGT ++ D++TD+   S+  E+  E      H G +++AR+   
Sbjct: 358 FIVVLDHRKESVVVAVRGTMSLQDVLTDLSAESENLELDIELQDCVAHKGIAQAARYIYR 417

Query: 292 HEI--GMIRRCLEKYQVGFRLRLVGHSLGGATASLLAVMLRKMSNKELGFSPDIVSAIGF 351
             +  G++ +        +RL +VGHSLG   A+LLA+MLR       G  P  V A  F
Sbjct: 418 RLVNDGILSQAF-SVAPEYRLVVVGHSLGAGAAALLAIMLR-------GAYPQ-VRAYAF 477

Query: 352 ATP-PCVSRKLAESCADYVTTVVMQDDVIPRLSVASLTRLRIEILQ 393
           + P   +S+ L E   D+V ++++  DVIPRLSVA++  L+  IL+
Sbjct: 478 SPPRGLLSKSLFEYSKDFVVSLILGMDVIPRLSVANMEDLKRRILR 514

[Close]

BLAST of Spg014527 vs. ExPASy Swiss-Prot
Match: Q8NCG7 (Diacylglycerol lipase-beta OS=Homo sapiens OX=9606 GN=DAGLB PE=1 SV=2)

HSP 1 Score: 75.1 bits (183), Expect = 2.8e-12
Identity = 55/166 (33.13%), Postives = 89/166 (53.61%), Query Frame = 0


Query: 232 YYIGVDTRKKLVIFGIRGTRTVYDLITDIITSSDREEVTFEGYS--THFGTSESARWFLD 291
           + + +D RK+ V+  +RGT ++ D++TD+   S+  +V  E      H G S++AR+   
Sbjct: 358 FLVALDHRKESVVVAVRGTMSLQDVLTDLSAESEVLDVECEVQDRLAHKGISQAARYVYQ 417

Query: 292 HEI--GMIRRCLEKYQVGFRLRLVGHSLGGATASLLAVMLRKMSNKELGFSPDIVSAIGF 351
             I  G++ +        +RL +VGHSLGG  A+LLA MLR    +        V    F
Sbjct: 418 RLINDGILSQAF-SIAPEYRLVIVGHSLGGGAAALLATMLRAAYPQ--------VRCYAF 477

Query: 352 ATPPCV-SRKLAESCADYVTTVVMQDDVIPRLSVASLTRLRIEILQ 393
           + P  + S+ L E    ++ ++V+  DVIPRLSV +L  L+  IL+
Sbjct: 478 SPPRGLWSKALQEYSQSFIVSLVLGKDVIPRLSVTNLEDLKRRILR 514

[Close]

BLAST of Spg014527 vs. ExPASy Swiss-Prot
Match: Q91WC9 (Diacylglycerol lipase-beta OS=Mus musculus OX=10090 GN=Daglb PE=1 SV=2)

HSP 1 Score: 75.1 bits (183), Expect = 2.8e-12
Identity = 58/193 (30.05%), Postives = 101/193 (52.33%), Query Frame = 0


Query: 205 AMLARRTMLRECNILKFVKDSSVMRPGYYIGVDTRKKLVIFGIRGTRTVYDLITDIITSS 264
           A + + T L+  + +       V    + + +D RK+ V+  +RGT ++ D++TD+   S
Sbjct: 331 ASILKTTGLQYRDFIHISFHDKVYELPFIVVLDHRKESVVVAVRGTMSLQDVLTDLSAES 390

Query: 265 DREE--VTFEGYSTHFGTSESARWFLDHEI--GMIRRCLEKYQVGFRLRLVGHSLGGATA 324
           +  E  +  +    H G +++AR+     +  G++ +        ++L LVGHSLG   A
Sbjct: 391 ETLELGIELQDCVAHKGIAQAARYIHRRLVNDGILSQAF-SVAPEYQLVLVGHSLGAGAA 450

Query: 325 SLLAVMLRKMSNKELGFSPDIVSAIGFATP-PCVSRKLAESCADYVTTVVMQDDVIPRLS 384
           +LLA+MLR       G  P  V A  F+ P   +S+ L E   D+V ++++  DVIPRLS
Sbjct: 451 ALLAIMLR-------GAYPQ-VRAYAFSPPRGLLSKSLYEYSKDFVVSLILGMDVIPRLS 510

Query: 385 VASLTRLRIEILQ 393
           V ++  L+  IL+
Sbjct: 511 VTNMEDLKRRILR 514

[Close]

BLAST of Spg014527 vs. ExPASy Swiss-Prot
Match: Q9Y4D2 (Diacylglycerol lipase-alpha OS=Homo sapiens OX=9606 GN=DAGLA PE=1 SV=3)

HSP 1 Score: 63.5 bits (153), Expect = 8.5e-09
Identity = 55/179 (30.73%), Postives = 90/179 (50.28%), Query Frame = 0


Query: 226 SVMRPGYYIGVDTRKKLVIFGIRGTRTVYDLITDIITSSDREEVTFEGYS----THFGTS 285
           +V    +Y+ VD  KK V+  IRGT +  D +TD+  + D E +  EG+      H G  
Sbjct: 376 AVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDL--TGDAERLPVEGHHGTWLGHKGMV 435

Query: 286 ESARWF---LDHEIGMIR---RCLEKYQVGFRLRLVGHSLGGATASLLAVMLRKMSNKEL 345
            SA +    L+ E+ + +   R L +    + L +VGHSLG  TA++L+ +LR       
Sbjct: 436 LSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLR------- 495

Query: 346 GFSPDIVSAIGFA-TPP--CVSRKLAESCADYVTTVVMQDDVIPRLSVASLTRLRIEIL 392
              P   +   FA +PP   +S    E   ++VT VV+  D++PR+ ++ L   R ++L
Sbjct: 496 ---PQYPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLL 542

[Close]

BLAST of Spg014527 vs. ExPASy Swiss-Prot
Match: Q6WQJ1 (Diacylglycerol lipase-alpha OS=Mus musculus OX=10090 GN=Dagla PE=1 SV=2)

HSP 1 Score: 63.5 bits (153), Expect = 8.5e-09
Identity = 55/179 (30.73%), Postives = 90/179 (50.28%), Query Frame = 0


Query: 226 SVMRPGYYIGVDTRKKLVIFGIRGTRTVYDLITDIITSSDREEVTFEGYS----THFGTS 285
           +V    +Y+ VD  KK V+  IRGT +  D +TD+  + D E +  EG+      H G  
Sbjct: 376 AVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDL--TGDAERLPVEGHRGTWLGHKGMV 435

Query: 286 ESARWF---LDHEIGMIR---RCLEKYQVGFRLRLVGHSLGGATASLLAVMLRKMSNKEL 345
            SA +    L+ E+ + +   R L +    + L +VGHSLG  TA++L+ +LR       
Sbjct: 436 LSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLR------- 495

Query: 346 GFSPDIVSAIGFA-TPP--CVSRKLAESCADYVTTVVMQDDVIPRLSVASLTRLRIEIL 392
              P   +   FA +PP   +S    E   ++VT VV+  D++PR+ ++ L   R ++L
Sbjct: 496 ---PQYPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLL 542

[Close]

BLAST of Spg014527 vs. ExPASy TrEMBL
Match: A0A6J1H649 (sn1-specific diacylglycerol lipase beta OS=Cucurbita moschata OX=3662 GN=LOC111460812 PE=4 SV=1)

HSP 1 Score: 878.2 bits (2268), Expect = 1.8e-251
Identity = 466/564 (82.62%), Postives = 497/564 (88.12%), Query Frame = 0


Query: 1   MASRTARRLLHNFHYARSFLRKQEWQASSVTVNSVLLHFQRSKADKVGQGVHKEGEVLPG 60
           M++ TARRLLHN + ARS LRKQ+WQASSVTVNSVL+H QRSKAD V   V KE EVLP 
Sbjct: 1   MSNGTARRLLHNLYSARSSLRKQKWQASSVTVNSVLMHLQRSKADMVENNVRKENEVLPE 60

Query: 61  VSVRSEMENTSKKGSKPTKYFSDAEDISDSNRRLDLAWLTVTKALEPALQLFRWALSSGD 120
           VSVRSEME  SK+GSK  KYFSDAEDISDSNRRL+LAWL  TKAL+ A QL+RWALSSGD
Sbjct: 61  VSVRSEMEKNSKEGSKTPKYFSDAEDISDSNRRLELAWL--TKALKSASQLYRWALSSGD 120

Query: 121 EIQNQIPPRSRSVSEIIASIQRSKMGIQDWSLSDLTIGLCLIYLRQASTNPLEDVKGVQI 180
            IQNQI PRSRSVSEIIASIQRSKMGIQ+WSLSDLTIGL LIYLRQASTNPL D+KGVQI
Sbjct: 121 GIQNQIQPRSRSVSEIIASIQRSKMGIQEWSLSDLTIGLFLIYLRQASTNPLADIKGVQI 180

Query: 181 SSDAIVEDLIYYLELADGSYKDSAAMLARRTMLRECNILKFVKDSSVMRPGYYIGVDTRK 240
           +S+AIVEDLIYYLELA G YKDSAAMLAR TMLRE NILKF+KDSSVMRPGYYIGVDTRK
Sbjct: 181 TSNAIVEDLIYYLELAKGCYKDSAAMLARNTMLREPNILKFIKDSSVMRPGYYIGVDTRK 240

Query: 241 KLVIFGIRGTRTVYDLITDIITSSDREEVTFEGYSTHFGTSESARWFLDHEIGMIRRCLE 300
           KLVIFGIRGT +VYDLITDI TSSD EEVT EGYSTHFGTSESARWFLDHEI MIRRCL+
Sbjct: 241 KLVIFGIRGTHSVYDLITDITTSSD-EEVTLEGYSTHFGTSESARWFLDHEIEMIRRCLK 300

Query: 301 KYQVGFRLRLVGHSLGGATASLLAVMLRKMSNKELGFSPDIVSAIGFATPPCVSRKLAES 360
           KYQ GFRLRLVGHSLGGA ASLLAVMLRK SNKELGFSPDIVSAIGFATPPCVSRKLAES
Sbjct: 301 KYQ-GFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFSPDIVSAIGFATPPCVSRKLAES 360

Query: 361 CADYVTTVVMQDDVIPRLSVASLTRLRIEILQTDWMSVIEKQDLKGIVGLVTNAKQVVTS 420
           CADYVTTVVMQDD+IPRLS+ASLT+LR+EILQTDW SVIE+QD K I GL+TNAKQVVTS
Sbjct: 361 CADYVTTVVMQDDIIPRLSLASLTKLRVEILQTDWTSVIEEQDWKNIYGLLTNAKQVVTS 420

Query: 421 VQDVARKLADFAKFTSKKRSSGKPILMFTDDSNRKESHVASGPPPSHPTAALQSVAAAEN 480
           VQDVARKL D+AKFTSKK  S        DDS  K SHVASGPPPS PTA +Q    A+N
Sbjct: 421 VQDVARKLGDYAKFTSKKGPS--------DDSYGKGSHVASGPPPSQPTALVQRADTAQN 480

Query: 481 EAAKCKIPEELFVPGTVYYLKRH--MDSTPEYFTLWKRHPDEHFQQIVLSSNLISNHKCD 540
           EAA+CKIPEELFVPGTVYYLK+H  ++   EYFTLWKRHPDEHFQQIVLSSNLIS+HKCD
Sbjct: 481 EAARCKIPEELFVPGTVYYLKKHVNLEEAYEYFTLWKRHPDEHFQQIVLSSNLISDHKCD 540

Query: 541 SHYYALRDVLKSLPSCNDEEGIFS 563
           SHY+ALRDVLK LPSCND EGIFS
Sbjct: 541 SHYHALRDVLKGLPSCND-EGIFS 551

[Close]

BLAST of Spg014527 vs. ExPASy TrEMBL
Match: A0A6J1KT65 (sn1-specific diacylglycerol lipase beta OS=Cucurbita maxima OX=3661 GN=LOC111498003 PE=4 SV=1)

HSP 1 Score: 875.9 bits (2262), Expect = 8.8e-251
Identity = 465/564 (82.45%), Postives = 496/564 (87.94%), Query Frame = 0


Query: 1   MASRTARRLLHNFHYARSFLRKQEWQASSVTVNSVLLHFQRSKADKVGQGVHKEGEVLPG 60
           M++ TARRLL N + ARS+LRKQ+WQASSVTVNSVL+H QRSKAD V   V KE EVLP 
Sbjct: 1   MSNGTARRLLRNLYSARSYLRKQKWQASSVTVNSVLMHLQRSKADMVENNVRKENEVLPE 60

Query: 61  VSVRSEMENTSKKGSKPTKYFSDAEDISDSNRRLDLAWLTVTKALEPALQLFRWALSSGD 120
           VSVRSEME   KKGSK  KYFSDAEDISDSNRRL+LAWL  TKAL+ A QL+RWALSSGD
Sbjct: 61  VSVRSEMEKNIKKGSKTPKYFSDAEDISDSNRRLELAWL--TKALKSASQLYRWALSSGD 120

Query: 121 EIQNQIPPRSRSVSEIIASIQRSKMGIQDWSLSDLTIGLCLIYLRQASTNPLEDVKGVQI 180
            IQNQI PRSRSVSEIIASIQRSKMGIQ+WSLSDLTIGL LIYLRQASTNPL D+KGVQI
Sbjct: 121 GIQNQIQPRSRSVSEIIASIQRSKMGIQEWSLSDLTIGLFLIYLRQASTNPLADIKGVQI 180

Query: 181 SSDAIVEDLIYYLELADGSYKDSAAMLARRTMLRECNILKFVKDSSVMRPGYYIGVDTRK 240
           +S+AIVEDLIYYLELA G YKDSAAMLAR TMLRE NILKF+KDSSVMRPGYYIGVDTRK
Sbjct: 181 TSNAIVEDLIYYLELAKGCYKDSAAMLARNTMLREPNILKFIKDSSVMRPGYYIGVDTRK 240

Query: 241 KLVIFGIRGTRTVYDLITDIITSSDREEVTFEGYSTHFGTSESARWFLDHEIGMIRRCLE 300
           KLVIFGIRGT +VYDLITDI TSSD EEVTFEGYSTHFGTSESARWFLDHEI MIR+CL+
Sbjct: 241 KLVIFGIRGTHSVYDLITDITTSSD-EEVTFEGYSTHFGTSESARWFLDHEIEMIRQCLK 300

Query: 301 KYQVGFRLRLVGHSLGGATASLLAVMLRKMSNKELGFSPDIVSAIGFATPPCVSRKLAES 360
           KYQ GFRLRLVGHSLGGA ASLLAVMLRK SNKELGF PDIVSAIGFATPPCVSRKLAES
Sbjct: 301 KYQ-GFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFRPDIVSAIGFATPPCVSRKLAES 360

Query: 361 CADYVTTVVMQDDVIPRLSVASLTRLRIEILQTDWMSVIEKQDLKGIVGLVTNAKQVVTS 420
           CADYVTTVVMQDD+IPRLS+ASLT+LRIEILQTDW SVIE+QD K I GL+TNAKQVVTS
Sbjct: 361 CADYVTTVVMQDDIIPRLSLASLTKLRIEILQTDWTSVIEEQDWKNIYGLLTNAKQVVTS 420

Query: 421 VQDVARKLADFAKFTSKKRSSGKPILMFTDDSNRKESHVASGPPPSHPTAALQSVAAAEN 480
           VQDVARKL D+AKFTSKK  S        DDS  K SHVASGPPPS PTA +Q    A+N
Sbjct: 421 VQDVARKLGDYAKFTSKKGPS--------DDSYGKGSHVASGPPPSQPTAVVQRADTAQN 480

Query: 481 EAAKCKIPEELFVPGTVYYLKRH--MDSTPEYFTLWKRHPDEHFQQIVLSSNLISNHKCD 540
           EAA+CKIPEELFVPGTVYYLK+H  ++   EYFTLWKRHPDEHFQQIVLSSNLIS+HKCD
Sbjct: 481 EAARCKIPEELFVPGTVYYLKKHVNLEEAYEYFTLWKRHPDEHFQQIVLSSNLISDHKCD 540

Query: 541 SHYYALRDVLKSLPSCNDEEGIFS 563
           SHY+ALRDVLK LPSCND EGIFS
Sbjct: 541 SHYHALRDVLKGLPSCND-EGIFS 551

[Close]

BLAST of Spg014527 vs. ExPASy TrEMBL
Match: A0A6J1DSG2 (uncharacterized protein LOC111023479 OS=Momordica charantia OX=3673 GN=LOC111023479 PE=4 SV=1)

HSP 1 Score: 859.8 bits (2220), Expect = 6.5e-246
Identity = 462/563 (82.06%), Postives = 494/563 (87.74%), Query Frame = 0


Query: 1   MASRTARRLLHNFHYARSFLRKQEWQASSVTVNSVLLHFQRSKADKVGQGVHKEGEVLPG 60
           MAS   RRLLHNFH+ARSFLRKQE QASSVTVNSVL+HF RSKADKV  GVHKEGEVLP 
Sbjct: 1   MASGAVRRLLHNFHHARSFLRKQELQASSVTVNSVLMHFHRSKADKVEIGVHKEGEVLPE 60

Query: 61  VSVRSEMENTSKKGSKPTKYFSDAEDISDSNRRLDLAWLTVTKALEPALQLFRWALS-SG 120
           VSVRSEME  SKKGS+PTKYFSD E+ISDS RR +LAWL  TKALEPALQL+RWALS SG
Sbjct: 61  VSVRSEMEKNSKKGSEPTKYFSDDENISDSKRRSELAWL--TKALEPALQLYRWALSASG 120

Query: 121 DEIQNQIPPRSRSVSEIIASIQRSKMGIQDWSLSDLTIGLCLIYLRQASTNPLEDVKGVQ 180
           D I++Q PPRSRSVSEIIASIQ SKMGIQDWSLSDLTIGL LIYLRQASTNP ED+ GVQ
Sbjct: 121 DGIRDQTPPRSRSVSEIIASIQWSKMGIQDWSLSDLTIGLYLIYLRQASTNPFEDISGVQ 180

Query: 181 ISSDAIVEDLIYYLELADGSYKDSAAMLARRTMLRECNILKFVKDSSVMRPGYYIGVDTR 240
           ISSDAIV+DLIYYLELA GSYKDSAA LAR +MLRECNILKFV DSSVMRPGYYIGVDTR
Sbjct: 181 ISSDAIVQDLIYYLELAKGSYKDSAASLARNSMLRECNILKFVGDSSVMRPGYYIGVDTR 240

Query: 241 KKLVIFGIRGTRTVYDLITDIITSSDREEVTFEGYSTHFGTSESARWFLDHEIGMIRRCL 300
           KKLVIFGIRGT T+ DLITDI+TSSD  EVTFEGY THFGT+ESARWFLDHE+GMIRRCL
Sbjct: 241 KKLVIFGIRGTHTLSDLITDIVTSSD-GEVTFEGYVTHFGTAESARWFLDHEMGMIRRCL 300

Query: 301 EKYQVGFRLRLVGHSLGGATASLLAVMLRKMSNKELGFSPDIVSAIGFATPPCVSRKLAE 360
           EKYQ GFRLRLVGHSLGGA ASLLAVMLRK SNKELGFSPDIVSAIG+ATPPCVSRKLAE
Sbjct: 301 EKYQ-GFRLRLVGHSLGGAIASLLAVMLRKKSNKELGFSPDIVSAIGYATPPCVSRKLAE 360

Query: 361 SCADYVTTVVMQDDVIPRLSVASLTRLRIEILQTDWMSVIEKQDLKGIVGLVTNAKQVVT 420
           SCADYVTTVVMQDD+IPRLS+ASLTRLR EILQTDW SVIEKQD K  +GLVTNA+QVV+
Sbjct: 361 SCADYVTTVVMQDDIIPRLSIASLTRLRNEILQTDWTSVIEKQDWKSFIGLVTNARQVVS 420

Query: 421 SVQDVARKLADFAKFTSKKRSSGKPILMFTDDSNRKESHVASGPPPSHPTAALQSVAAAE 480
           SVQDVARKLAD+AKFTSKK SS        D S RKESHVAS PP  HPTAA ++ AA  
Sbjct: 421 SVQDVARKLADYAKFTSKKISS--------DGSIRKESHVASAPP--HPTAAARNKAA-- 480

Query: 481 NEAAKCKIPEELFVPGTVYYLKRHMDSTPEYFTLWKRHPDEHFQQIVLSSNLISNHKCDS 540
              A+ K PEELFVPGTVYYLKR+ + TPEYFTLWKRHP EHFQ+IVLSSNLIS+H+CDS
Sbjct: 481 --TARYKTPEELFVPGTVYYLKRNTNGTPEYFTLWKRHPGEHFQRIVLSSNLISDHRCDS 540

Query: 541 HYYALRDVLKSLPSCNDEEGIFS 563
           HYYALRDVLK LP+ +D E IFS
Sbjct: 541 HYYALRDVLKGLPARSD-ESIFS 544

[Close]

BLAST of Spg014527 vs. ExPASy TrEMBL
Match: A0A5D3D3Q9 (Alpha/beta-Hydrolases superfamily protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold306G003180 PE=4 SV=1)

HSP 1 Score: 849.0 bits (2192), Expect = 1.2e-242
Identity = 448/559 (80.14%), Postives = 493/559 (88.19%), Query Frame = 0


Query: 1   MASRTARRLLHNFHYARSFLRKQEWQASSVTVNSVLLHFQRSKADKVGQGVHKEGEVLPG 60
           MA+ TARRL+HNFHYAR+FLRKQEWQA SVTVN+VL+HFQ SK DKV   V KEGEVL  
Sbjct: 1   MATGTARRLIHNFHYARNFLRKQEWQARSVTVNNVLMHFQSSKVDKVENDVIKEGEVLRE 60

Query: 61  VSVRSEMENTSKKGSKPTKYFSDAEDISDSNRRLDLAWLTVTKALEPALQLFRWALSSGD 120
           VS+RSEME  S    K ++Y    ED +DSN R +LAWL  TK LEPALQL+RWALSSGD
Sbjct: 61  VSLRSEMEKNSTIDCKRSEY---VEDSTDSNWRSELAWL--TKTLEPALQLYRWALSSGD 120

Query: 121 EIQNQIPPRSRSVSEIIASIQRSKMGIQDWSLSDLTIGLCLIYLRQASTNPLEDVKGVQI 180
                IP RSRSVSEIIASIQRSK GIQDWSLSDLTIGLCLIYLRQASTNPLED+KGVQI
Sbjct: 121 ----GIPHRSRSVSEIIASIQRSKTGIQDWSLSDLTIGLCLIYLRQASTNPLEDLKGVQI 180

Query: 181 SSDAIVEDLIYYLELADGSYKDSAAMLARRTMLRECNILKFVKDSSVMRPGYYIGVDTRK 240
           SS+AIVEDLIY++ELA+GSYK+S +MLA+ TMLRECNILKFVK+SSVMRPGYYIGVDTRK
Sbjct: 181 SSNAIVEDLIYFVELAEGSYKNSTSMLAKTTMLRECNILKFVKNSSVMRPGYYIGVDTRK 240

Query: 241 KLVIFGIRGTRTVYDLITDIITSSDREEVTFEGYSTHFGTSESARWFLDHEIGMIRRCLE 300
           KLVIFGIRGT TVYDLITDIIT+SDR +VTFEGYSTHFGTSESA+WFL +EIGMIRRCLE
Sbjct: 241 KLVIFGIRGTHTVYDLITDIITTSDR-DVTFEGYSTHFGTSESAQWFLQNEIGMIRRCLE 300

Query: 301 KYQVGFRLRLVGHSLGGATASLLAVMLRKMSNKELGFSPDIVSAIGFATPPCVSRKLAES 360
           KYQVGFRLRLVGHSLGGA ASLLAVMLRK S KELGFSPDIVSAIGF TPPCVSRKLAES
Sbjct: 301 KYQVGFRLRLVGHSLGGAIASLLAVMLRKKSKKELGFSPDIVSAIGFGTPPCVSRKLAES 360

Query: 361 CADYVTTVVMQDDVIPRLSVASLTRLRIEILQTDWMSVIEKQDLKGIVGLVTNAKQVVTS 420
           CADYVTTVVMQDDVIPRLSVASLTRLRIEILQTDWMS+I K+D K I+GLVTNAKQVVTS
Sbjct: 361 CADYVTTVVMQDDVIPRLSVASLTRLRIEILQTDWMSLIGKEDWKSIIGLVTNAKQVVTS 420

Query: 421 VQDVARKLADFAKFTSKKRSSGKPILMFTDDSNRKESHVASGPPPSHPTAALQSVAAAEN 480
           VQDVA+KLAD+AKFTSKK+SS        DD+N+KES VASG P S  T+ALQS  AA+N
Sbjct: 421 VQDVAQKLADYAKFTSKKKSS--------DDNNKKESDVASGSPRSCATSALQSATAAQN 480

Query: 481 EAAKCKIPEELFVPGTVYYLKRHMDSTPEYFTLWKRHPDEHFQQIVLSSNLISNHKCDSH 540
           +AA+CKI +ELF+PGTVYYLKRH+DSTPEYF+LWKRHPDEHFQQIVLS+ L+S+HKCDSH
Sbjct: 481 KAARCKISDELFIPGTVYYLKRHVDSTPEYFSLWKRHPDEHFQQIVLSNILLSDHKCDSH 540

Query: 541 YYALRDVLKSLP--SCNDE 558
           YYALRDVLK LP  SC++E
Sbjct: 541 YYALRDVLKGLPISSCSNE 541

[Close]

BLAST of Spg014527 vs. ExPASy TrEMBL
Match: A0A5A7TF50 (Alpha/beta-Hydrolases superfamily protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold67G005250 PE=4 SV=1)

HSP 1 Score: 842.8 bits (2176), Expect = 8.3e-241
Identity = 447/559 (79.96%), Postives = 492/559 (88.01%), Query Frame = 0


Query: 1   MASRTARRLLHNFHYARSFLRKQEWQASSVTVNSVLLHFQRSKADKVGQGVHKEGEVLPG 60
           MA+ TARRL+HNFHYAR+FLRKQEWQA SVTVN+VL+HFQ SK DKV   V KEGEVL  
Sbjct: 1   MATGTARRLIHNFHYARNFLRKQEWQARSVTVNNVLMHFQSSKVDKVENDVIKEGEVLRE 60

Query: 61  VSVRSEMENTSKKGSKPTKYFSDAEDISDSNRRLDLAWLTVTKALEPALQLFRWALSSGD 120
           VS+RSEME  S    K ++Y    ED +DSN R +LAWL  TK LEPALQL+RWALSSGD
Sbjct: 61  VSLRSEMEKNSTIDCKRSEY---VEDSTDSNWRSELAWL--TKTLEPALQLYRWALSSGD 120

Query: 121 EIQNQIPPRSRSVSEIIASIQRSKMGIQDWSLSDLTIGLCLIYLRQASTNPLEDVKGVQI 180
                IP RSRSVSEIIASIQRSK GIQDWSLSDLTIGLCLIYLRQASTNPLED+KGVQI
Sbjct: 121 ----GIPHRSRSVSEIIASIQRSKTGIQDWSLSDLTIGLCLIYLRQASTNPLEDLKGVQI 180

Query: 181 SSDAIVEDLIYYLELADGSYKDSAAMLARRTMLRECNILKFVKDSSVMRPGYYIGVDTRK 240
           SS+AIVEDLIY++ELA+GSYK+S +MLA+ TMLRECNILKFVK+SSVMRPGYYIGVDTRK
Sbjct: 181 SSNAIVEDLIYFVELAEGSYKNSTSMLAKTTMLRECNILKFVKNSSVMRPGYYIGVDTRK 240

Query: 241 KLVIFGIRGTRTVYDLITDIITSSDREEVTFEGYSTHFGTSESARWFLDHEIGMIRRCLE 300
           KLVIFGIRGT TVYDLITDIIT+SDR +VTFEGYSTHFGTSESA+WFL +EIGMIRRCLE
Sbjct: 241 KLVIFGIRGTHTVYDLITDIITTSDR-DVTFEGYSTHFGTSESAQWFLQNEIGMIRRCLE 300

Query: 301 KYQVGFRLRLVGHSLGGATASLLAVMLRKMSNKELGFSPDIVSAIGFATPPCVSRKLAES 360
           KYQ GFRLRLVGHSLGGA ASLLAVMLRK S KELGFSPDIVSAIGF TPPCVSRKLAES
Sbjct: 301 KYQ-GFRLRLVGHSLGGAIASLLAVMLRKKSKKELGFSPDIVSAIGFGTPPCVSRKLAES 360

Query: 361 CADYVTTVVMQDDVIPRLSVASLTRLRIEILQTDWMSVIEKQDLKGIVGLVTNAKQVVTS 420
           CADYVTTVVMQDDVIPRLSVASLTRLRIEILQTDWMS+I K+D K I+GLVTNAKQVVTS
Sbjct: 361 CADYVTTVVMQDDVIPRLSVASLTRLRIEILQTDWMSLIGKEDWKSIIGLVTNAKQVVTS 420

Query: 421 VQDVARKLADFAKFTSKKRSSGKPILMFTDDSNRKESHVASGPPPSHPTAALQSVAAAEN 480
           VQDVA+KLAD+AKFTSKK+SS        DD+N+KES VASG P S  T+ALQS  AA+N
Sbjct: 421 VQDVAQKLADYAKFTSKKKSS--------DDNNKKESDVASGSPRSCATSALQSATAAQN 480

Query: 481 EAAKCKIPEELFVPGTVYYLKRHMDSTPEYFTLWKRHPDEHFQQIVLSSNLISNHKCDSH 540
           +AA+CKI +ELF+PGTVYYLKRH+DSTPEYF+LWKRHPDEHFQQIVLS+ L+S+HKCDSH
Sbjct: 481 KAARCKISDELFIPGTVYYLKRHVDSTPEYFSLWKRHPDEHFQQIVLSNILLSDHKCDSH 540

Query: 541 YYALRDVLKSLP--SCNDE 558
           YYALRDVLK LP  SC++E
Sbjct: 541 YYALRDVLKGLPISSCSNE 540

[Close]

BLAST of Spg014527 vs. TAIR 10
Match: AT2G42450.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 577.8 bits (1488), Expect = 9.5e-165
Identity = 324/573 (56.54%), Postives = 401/573 (69.98%), Query Frame = 0


Query: 1   MASRTARRLLHNFHYARSFLRKQEWQASSVTVNSVLLHFQRSKADKVGQGVHKEGEVLPG 60
           MA+R   RLL NF    S  RK+      V  N V+  FQ S   + G+ V KE E    
Sbjct: 1   MANRALNRLLRNFFVESSPFRKRSGLYQGVVYN-VIKRFQ-SHLSESGK-VEKELEEQEA 60

Query: 61  VSVRSEMENTSKKGSKPTKYF--------SDAEDISDSNRRLDLAWLTVTKALEPALQLF 120
              R     +S+ G +  + F        S  ED  D   +LDLAWL  TKALEPA+QL 
Sbjct: 61  EKRRPSSVTSSRSGLEKRRQFESGIDNDSSSDEDTGDGKWKLDLAWL--TKALEPAMQLR 120

Query: 121 RWALSSGDEIQNQIPPRSRSVSEIIASIQRSKMGIQDWSLSDLTIGLCLIYLRQASTNPL 180
           RWAL +G++      P SRS+SEIIASIQRS++GI+ W+  DLTIGL LIYLRQAS +P 
Sbjct: 121 RWALPTGEKSL----PGSRSLSEIIASIQRSRLGIEGWTFGDLTIGLYLIYLRQASLSPF 180

Query: 181 EDVKGVQISSDAIVEDLIYYLELADGSYKDSAAMLARRTMLRECNILKFVKDSSVMRPGY 240
           EDVKGV++ S++ V DLIY  ELA G Y+DS + LA+ TMLRE NILKFVKDSSVMRPGY
Sbjct: 181 EDVKGVEVVSESTVYDLIYNAELAKGCYRDSVSGLAKNTMLRENNILKFVKDSSVMRPGY 240

Query: 241 YIGVDTRKKLVIFGIRGTRTVYDLITDIITSSDREEVTFEGYSTHFGTSESARWFLDHEI 300
           YIGVD R+KLV+FGIRGT T+YDLITDI++SSD EEVTFEGYSTHFGT+E+ARWFL+HE+
Sbjct: 241 YIGVDHRRKLVVFGIRGTHTIYDLITDIVSSSD-EEVTFEGYSTHFGTAEAARWFLNHEL 300

Query: 301 GMIRRCLEKYQVGFRLRLVGHSLGGATASLLAVMLRKMSNKELGFSPDIVSAIGFATPPC 360
             IRRCL KY+ G++LRLVGHSLGGA ASL+A+ML+KM  +ELGF  +I+SA+G+ATPPC
Sbjct: 301 QTIRRCLAKYE-GYKLRLVGHSLGGAIASLMAIMLKKMPREELGFDAEIISAVGYATPPC 360

Query: 361 VSRKLAESCADYVTTVVMQDDVIPRLSVASLTRLRIEILQTDWMSVIEKQDLKGIVGLVT 420
           VS++LAE+C+++VTT+VMQDD+IPRLS ASL RLR EILQTDW SVIEK++ K ++ LVT
Sbjct: 361 VSKELAENCSEFVTTIVMQDDIIPRLSAASLARLRNEILQTDWTSVIEKEEWKNVLDLVT 420

Query: 421 NAKQVVTSVQDVARKLADFAKFTSKKRSSGKPILMFTDDSNRKESHVASGPPPSHPTAAL 480
           NAKQVVTSVQDVARK++D+A F +KK     P       S   +S +      +      
Sbjct: 421 NAKQVVTSVQDVARKVSDYANFGNKKEVPEIP------SSKNNQSGILISDSTTKDVV-- 480

Query: 481 QSVAAAENEAAKCKIPEELFVPGTVYYLKRHMDSTP--------EYFTLWKRHPDEHFQQ 540
                        K+PEEL+VPG VYYL R +  TP        EY++LWKR P +HFQ+
Sbjct: 481 -------------KLPEELYVPGAVYYLMRSLRGTPKSSAGKQVEYYSLWKRDPGQHFQR 540

Query: 541 IVLSSNLISNHKCDSHYYALRDVLKSLPSCNDE 558
           I+LS N I++HKCDSHYYALRDVLK  PS  +E
Sbjct: 541 ILLSGNFITDHKCDSHYYALRDVLKGFPSFINE 541

[Close]

BLAST of Spg014527 vs. TAIR 10
Match: AT4G16070.1 (Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 )

HSP 1 Score: 92.4 bits (228), Expect = 1.2e-18
Identity = 89/361 (24.65%), Postives = 156/361 (43.21%), Query Frame = 0


Query: 150 WSLSDLTIGLCLIYLRQ---------ASTNPLEDVKGVQISSDAIVEDLIYYLELADGSY 209
           W ++DL  G+  +  RQ         A +N +E +KG +I  D  + +L+ +L L     
Sbjct: 81  WPIADLAFGINYLMRRQGNFPTASVYAGSNCIE-LKGPEIIMD--LTELLRFLTLCMLFS 140

Query: 210 KDSAAMLARRTMLRECNILKFVKDSSVMRPGYYIGVDTRKKLVIFGIRGTRTVYDLIT-- 269
           K   A+          ++L     + +M+P + I  DT  K ++  IRGT ++ D +T  
Sbjct: 141 KKPFAVFLESAGYTHEDVLLQKPKAGIMQPAFTIIRDTNSKCILLLIRGTHSIKDTLTAA 200

Query: 270 ---------DIITSSDREEVTFEGYSTHFGTSESARWFLDHEIGMIRRCLEKYQVGFRLR 329
                     ++       +   GY+ H G   +ARW     +  + + L++    F+++
Sbjct: 201 TGAVVPFHHSVLHDGGLSNLVL-GYA-HCGMVAAARWIAKLSVPCLLKALDE-NPSFKVQ 260

Query: 330 LVGHSLGGATASLLAVMLRKMSNKELGFSPDIVSAIGFATPPCVSRKLAESCADYVTTVV 389
           +VGHSLGG TASLL  +LR+   KE   +    +   FA   C++  LAES   ++TT++
Sbjct: 261 IVGHSLGGGTASLLTYILRE--QKEFASA----TCFTFAPAACMTWDLAESGKHFITTII 320

Query: 390 MQDDVIPRLSVASLTRLRIEILQTDWMSVIEKQDLKGIVGLVTNAKQVVTSVQDVARKLA 449
              D++P  S +S+  LR E+  + W       DL+  V        V  S   +  +L 
Sbjct: 321 NGSDLVPTFSASSVDDLRSEVTSSSW-----SNDLRDQVEHTRVLSVVYRSATAIGSRLP 380

Query: 450 DFAKFTSKKRSSG---KPILMFTDDSNRKESHVASGPPPSHPTAALQSVAAAENEAAKCK 488
             A   +K   +G   +P+   T    ++   VA     +  T +  S       A   +
Sbjct: 381 SIASAKAKVAGAGAILRPVSSGTQVMLKRAQDVAQAVVQTRSTLSSWSCIGPRRRAISSQ 424

[Close]

BLAST of Spg014527 vs. TAIR 10
Match: AT4G16070.2 (Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 )

HSP 1 Score: 87.4 bits (215), Expect = 3.9e-17
Identity = 86/361 (23.82%), Postives = 151/361 (41.83%), Query Frame = 0


Query: 150 WSLSDLTIGLCLIYLRQ---------ASTNPLEDVKGVQISSDAIVEDLIYYLELADGSY 209
           W ++DL  G+  +  RQ         A +N +E +KG +I  D  + +L+ +L L     
Sbjct: 81  WPIADLAFGINYLMRRQGNFPTASVYAGSNCIE-LKGPEIIMD--LTELLRFLTLCMLFS 140

Query: 210 KDSAAMLARRTMLRECNILKFVKDSSVMRPGYYIGVDTRKKLVIFGIRGTRTVYDLIT-- 269
           K   A+          ++L     + +M+P + I  DT  K ++  IRGT ++ D +T  
Sbjct: 141 KKPFAVFLESAGYTHEDVLLQKPKAGIMQPAFTIIRDTNSKCILLLIRGTHSIKDTLTAA 200

Query: 270 ---------DIITSSDREEVTFEGYSTHFGTSESARWFLDHEIGMIRRCLEKYQVGFRLR 329
                     ++       +   GY+ H G   +ARW     +  + + L++    F+++
Sbjct: 201 TGAVVPFHHSVLHDGGLSNLVL-GYA-HCGMVAAARWIAKLSVPCLLKALDE-NPSFKVQ 260

Query: 330 LVGHSLGGATASLLAVMLRKMSNKELGFSPDIVSAIGFATPPCVSRKLAESCADYVTTVV 389
           +VGHSLGG TASLL  +LR+                 FA+  C +   AES   ++TT++
Sbjct: 261 IVGHSLGGGTASLLTYILREQKE--------------FASATCFTFAPAESGKHFITTII 320

Query: 390 MQDDVIPRLSVASLTRLRIEILQTDWMSVIEKQDLKGIVGLVTNAKQVVTSVQDVARKLA 449
              D++P  S +S+  LR E+  + W       DL+  V        V  S   +  +L 
Sbjct: 321 NGSDLVPTFSASSVDDLRSEVTSSSW-----SNDLRDQVEHTRVLSVVYRSATAIGSRLP 380

Query: 450 DFAKFTSKKRSSG---KPILMFTDDSNRKESHVASGPPPSHPTAALQSVAAAENEAAKCK 488
             A   +K   +G   +P+   T    ++   VA     +  T +  S       A   +
Sbjct: 381 SIASAKAKVAGAGAILRPVSSGTQVMLKRAQDVAQAVVQTRSTLSSWSCIGPRRRAISSQ 416

[Close]

BLAST of Spg014527 vs. TAIR 10
Match: AT3G14075.1 (Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 )

HSP 1 Score: 84.3 bits (207), Expect = 3.3e-16
Identity = 88/372 (23.66%), Postives = 158/372 (42.47%), Query Frame = 0


Query: 150 WSLSDLTIGLCLIYLRQASTNPLEDVKG----VQISSDAIVEDLIYYLELADGSY---KD 209
           W + DL  G+  +  RQ   + ++ V G    V++    +  +L Y L L    +   K 
Sbjct: 83  WPIGDLAFGINFLIKRQGLLH-VDRVFGGKDSVELRGSEVATELKYLLHLLTLCWHFSKK 142

Query: 210 SAAMLARRTMLRECNILKFVKDSSVMRPGYYIGVDTRKKLVIFGIRGTRTVYDLI---TD 269
           S       T   + N+L     + +++P + + VD   K  +  IRGT ++ D +   T 
Sbjct: 143 SFPFFLEETGFTKENVLIHEPKAGILKPAFTVLVDHNTKYFLLLIRGTHSIKDTLTAATG 202

Query: 270 IITSSDREEVTFEGYS------THFGTSESARWFLDHEIGMIRRCLEKYQVGFRLRLVGH 329
            I       V   G S       H G   +AR         + + LE+Y   +++++VGH
Sbjct: 203 AIVPFHHTVVNERGVSNLVLGYAHCGMVAAARCIAKLATPCLLKGLEQYP-DYKIKIVGH 262

Query: 330 SLGGATASLLAVMLRKMSNKELGFSPDIVSAIGFATPPCVSRKLAESCADYVTTVVMQDD 389
           SLGG TA+LL  ++R+             + + FA   C++ +LA+S  D++ +V+   D
Sbjct: 263 SLGGGTAALLTYIMREQK------MLSTATCVTFAPAACMTWELADSGNDFIVSVINGAD 322

Query: 390 VIPRLSVASLTRLRIEILQTDWMSVIEKQDLKGIVGLVTNAKQVVTSVQDVARKLADFAK 449
           ++P  S A++  LR E+  + W++     DL+  +        V  S   +  +L   A 
Sbjct: 323 LVPTFSAAAVDDLRAEVTASAWLN-----DLRNQIEHTRILSTVYRSATALGSRLPSMAT 382

Query: 450 FTSKKRSSG---KPILMFTDDSNRKESHVASGPP----------PSHPTAALQSVAAAE- 490
             +K   +G   +P+   T    R+   + + P           P    +A QS++  + 
Sbjct: 383 AKAKVAGAGAMLRPVSSGTQVVMRRAQSMLTRPALSISSWSCMGPRRRASATQSISEHQL 441

[Close]

BLAST of Spg014527 vs. TAIR 10
Match: AT3G14075.2 (Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 )

HSP 1 Score: 84.3 bits (207), Expect = 3.3e-16
Identity = 88/372 (23.66%), Postives = 158/372 (42.47%), Query Frame = 0


Query: 150 WSLSDLTIGLCLIYLRQASTNPLEDVKG----VQISSDAIVEDLIYYLELADGSY---KD 209
           W + DL  G+  +  RQ   + ++ V G    V++    +  +L Y L L    +   K 
Sbjct: 83  WPIGDLAFGINFLIKRQGLLH-VDRVFGGKDSVELRGSEVATELKYLLHLLTLCWHFSKK 142

Query: 210 SAAMLARRTMLRECNILKFVKDSSVMRPGYYIGVDTRKKLVIFGIRGTRTVYDLI---TD 269
           S       T   + N+L     + +++P + + VD   K  +  IRGT ++ D +   T 
Sbjct: 143 SFPFFLEETGFTKENVLIHEPKAGILKPAFTVLVDHNTKYFLLLIRGTHSIKDTLTAATG 202

Query: 270 IITSSDREEVTFEGYS------THFGTSESARWFLDHEIGMIRRCLEKYQVGFRLRLVGH 329
            I       V   G S       H G   +AR         + + LE+Y   +++++VGH
Sbjct: 203 AIVPFHHTVVNERGVSNLVLGYAHCGMVAAARCIAKLATPCLLKGLEQYP-DYKIKIVGH 262

Query: 330 SLGGATASLLAVMLRKMSNKELGFSPDIVSAIGFATPPCVSRKLAESCADYVTTVVMQDD 389
           SLGG TA+LL  ++R+             + + FA   C++ +LA+S  D++ +V+   D
Sbjct: 263 SLGGGTAALLTYIMREQK------MLSTATCVTFAPAACMTWELADSGNDFIVSVINGAD 322

Query: 390 VIPRLSVASLTRLRIEILQTDWMSVIEKQDLKGIVGLVTNAKQVVTSVQDVARKLADFAK 449
           ++P  S A++  LR E+  + W++     DL+  +        V  S   +  +L   A 
Sbjct: 323 LVPTFSAAAVDDLRAEVTASAWLN-----DLRNQIEHTRILSTVYRSATALGSRLPSMAT 382

Query: 450 FTSKKRSSG---KPILMFTDDSNRKESHVASGPP----------PSHPTAALQSVAAAE- 490
             +K   +G   +P+   T    R+   + + P           P    +A QS++  + 
Sbjct: 383 AKAKVAGAGAMLRPVSSGTQVVMRRAQSMLTRPALSISSWSCMGPRRRASATQSISEHQL 441

The following BLAST results are available for this feature:

BLAST of Spg014527 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038897252.1	1.8e-253	83.30	uncharacterized protein LOC120085373 isoform X1 [Benincasa hispida]	[more]
XP_023513693.1	3.3e-252	82.80	sn1-specific diacylglycerol lipase beta [Cucurbita pepo subsp. pepo]	[more]
XP_022959891.1	3.7e-251	82.62	sn1-specific diacylglycerol lipase beta [Cucurbita moschata]	[more]
XP_023004816.1	1.8e-250	82.45	sn1-specific diacylglycerol lipase beta [Cucurbita maxima]	[more]
XP_022156622.1	1.4e-245	82.06	uncharacterized protein LOC111023479 [Momordica charantia]	[more]

BLAST of Spg014527 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P0C1S9	5.7e-13	33.73	Diacylglycerol lipase-beta OS=Rattus norvegicus OX=10116 GN=Daglb PE=1 SV=1	[more]
Q8NCG7	2.8e-12	33.13	Diacylglycerol lipase-beta OS=Homo sapiens OX=9606 GN=DAGLB PE=1 SV=2	[more]
Q91WC9	2.8e-12	30.05	Diacylglycerol lipase-beta OS=Mus musculus OX=10090 GN=Daglb PE=1 SV=2	[more]
Q9Y4D2	8.5e-09	30.73	Diacylglycerol lipase-alpha OS=Homo sapiens OX=9606 GN=DAGLA PE=1 SV=3	[more]
Q6WQJ1	8.5e-09	30.73	Diacylglycerol lipase-alpha OS=Mus musculus OX=10090 GN=Dagla PE=1 SV=2	[more]

BLAST of Spg014527 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H649	1.8e-251	82.62	sn1-specific diacylglycerol lipase beta OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1KT65	8.8e-251	82.45	sn1-specific diacylglycerol lipase beta OS=Cucurbita maxima OX=3661 GN=LOC111498...	[more]
A0A6J1DSG2	6.5e-246	82.06	uncharacterized protein LOC111023479 OS=Momordica charantia OX=3673 GN=LOC111023...	[more]
A0A5D3D3Q9	1.2e-242	80.14	Alpha/beta-Hydrolases superfamily protein OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A5A7TF50	8.3e-241	79.96	Alpha/beta-Hydrolases superfamily protein OS=Cucumis melo var. makuwa OX=1194695...	[more]

BLAST of Spg014527 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G42450.1	9.5e-165	56.54	alpha/beta-Hydrolases superfamily protein	[more]
AT4G16070.1	1.2e-18	24.65	Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3	[more]
AT4G16070.2	3.9e-17	23.82	Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3	[more]
AT3G14075.1	3.3e-16	23.66	Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3	[more]
AT3G14075.2	3.3e-16	23.66	Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 174..422 e-value: 1.2E-32 score: 115.1
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 187..381
IPR002921	Fungal lipase-like domain	PFAM	PF01764	Lipase_3	coord: 245..380 e-value: 2.7E-21 score: 75.9
None	No IPR available	PANTHER	PTHR47418	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 101..557
None	No IPR available	CDD	cd00519	Lipase_3	coord: 189..385 e-value: 1.22885E-37 score: 136.837

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg014527.1	Spg014527.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
molecular_function	GO:0016787	hydrolase activity