Spg014524 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg014524
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionrRNA N-glycosidase
Locationscaffold3: 36759802 .. 36760677 (-)
RNA-Seq ExpressionSpg014524
SyntenySpg014524
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGAGACTCTTACTTCCTCTGTTACTTCTCGCCATCTTCCCAAGAGCTCCCGCTGTCAAGGGCGACGTAAGCTTCCGTTTATCAGGCGCCACAACCAGTTCCTACGGAGTTTTCATTTCAAATCTCCGAAGGGCTCTTCCAAATGAAAAGCTTCTGTACAACATTCCTCTATTACGTTCCACTCTTCCAGGTGCACAACGCTACACACTCATCCACCTCTCCAACTATGCCGATCAAACCATCTCCGTCGCCGTCGACGTAACGAACGTCTACATAATGGGGTACCGCGCCGGCGACACATCCTATTTCTTCAACGAGGCCTCTGCAGCAGAAGCTGCAAAATACGTATTCAAAGATGCCAGAAGAAAAGTCACACTTCCATATTCTGGCAACTACGAAAGGCTTCAAATTGCTGCAGGCAAGATAAGAGAAAACATTCCCCTGGGCCTCCCGGCTTTGGACAGTGCCATTACCACCTTGTTTTACTACAACGCCAACTCTGCTGCGTCGGCGCTTCTCGTACTCATTCAGTCCACTGCCGAGGCTGCCAGGTATAAATTTATCGAGCAACAGATTGGGAAGCGTGTTGATAAAACGTTCCTGCCGAGCCTCGCGATTATAAGTTTGGAAAACAGCTGGTCGGCTCTCTCGAAACAGATTCAGATTGCGAGTACGAATAATGGACAATTTGAGAGCCCAGTGGTGCTTATAAATGGTAATAATGAGCGAGTTAGTATAACGAATGTTAATGCGGGAGTTGTGACATCCAACATTGCGTTGCTGCTGAACAGAAACAATGTTGTTGCAGCCATTGATGAGGATGATGTTCCTCGGACACTCGGCTTTGGATATGGAGGTTATGCTGTAGTGTAA

mRNA sequence

ATGATGAGACTCTTACTTCCTCTGTTACTTCTCGCCATCTTCCCAAGAGCTCCCGCTGTCAAGGGCGACGTAAGCTTCCGTTTATCAGGCGCCACAACCAGTTCCTACGGAGTTTTCATTTCAAATCTCCGAAGGGCTCTTCCAAATGAAAAGCTTCTGTACAACATTCCTCTATTACGTTCCACTCTTCCAGGTGCACAACGCTACACACTCATCCACCTCTCCAACTATGCCGATCAAACCATCTCCGTCGCCGTCGACGTAACGAACGTCTACATAATGGGGTACCGCGCCGGCGACACATCCTATTTCTTCAACGAGGCCTCTGCAGCAGAAGCTGCAAAATACGTATTCAAAGATGCCAGAAGAAAAGTCACACTTCCATATTCTGGCAACTACGAAAGGCTTCAAATTGCTGCAGGCAAGATAAGAGAAAACATTCCCCTGGGCCTCCCGGCTTTGGACAGTGCCATTACCACCTTGTTTTACTACAACGCCAACTCTGCTGCGTCGGCGCTTCTCGTACTCATTCAGTCCACTGCCGAGGCTGCCAGGTATAAATTTATCGAGCAACAGATTGGGAAGCGTGTTGATAAAACGTTCCTGCCGAGCCTCGCGATTATAAGTTTGGAAAACAGCTGGTCGGCTCTCTCGAAACAGATTCAGATTGCGAGTACGAATAATGGACAATTTGAGAGCCCAGTGGTGCTTATAAATGGTAATAATGAGCGAGTTAGTATAACGAATGTTAATGCGGGAGTTGTGACATCCAACATTGCGTTGCTGCTGAACAGAAACAATGTTGTTGCAGCCATTGATGAGGATGATGTTCCTCGGACACTCGGCTTTGGATATGGAGGTTATGCTGTAGTGTAA

Coding sequence (CDS)

ATGATGAGACTCTTACTTCCTCTGTTACTTCTCGCCATCTTCCCAAGAGCTCCCGCTGTCAAGGGCGACGTAAGCTTCCGTTTATCAGGCGCCACAACCAGTTCCTACGGAGTTTTCATTTCAAATCTCCGAAGGGCTCTTCCAAATGAAAAGCTTCTGTACAACATTCCTCTATTACGTTCCACTCTTCCAGGTGCACAACGCTACACACTCATCCACCTCTCCAACTATGCCGATCAAACCATCTCCGTCGCCGTCGACGTAACGAACGTCTACATAATGGGGTACCGCGCCGGCGACACATCCTATTTCTTCAACGAGGCCTCTGCAGCAGAAGCTGCAAAATACGTATTCAAAGATGCCAGAAGAAAAGTCACACTTCCATATTCTGGCAACTACGAAAGGCTTCAAATTGCTGCAGGCAAGATAAGAGAAAACATTCCCCTGGGCCTCCCGGCTTTGGACAGTGCCATTACCACCTTGTTTTACTACAACGCCAACTCTGCTGCGTCGGCGCTTCTCGTACTCATTCAGTCCACTGCCGAGGCTGCCAGGTATAAATTTATCGAGCAACAGATTGGGAAGCGTGTTGATAAAACGTTCCTGCCGAGCCTCGCGATTATAAGTTTGGAAAACAGCTGGTCGGCTCTCTCGAAACAGATTCAGATTGCGAGTACGAATAATGGACAATTTGAGAGCCCAGTGGTGCTTATAAATGGTAATAATGAGCGAGTTAGTATAACGAATGTTAATGCGGGAGTTGTGACATCCAACATTGCGTTGCTGCTGAACAGAAACAATGTTGTTGCAGCCATTGATGAGGATGATGTTCCTCGGACACTCGGCTTTGGATATGGAGGTTATGCTGTAGTGTAA

Protein sequence

MMRLLLPLLLLAIFPRAPAVKGDVSFRLSGATTSSYGVFISNLRRALPNEKLLYNIPLLRSTLPGAQRYTLIHLSNYADQTISVAVDVTNVYIMGYRAGDTSYFFNEASAAEAAKYVFKDARRKVTLPYSGNYERLQIAAGKIRENIPLGLPALDSAITTLFYYNANSAASALLVLIQSTAEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLINGNNERVSITNVNAGVVTSNIALLLNRNNVVAAIDEDDVPRTLGFGYGGYAVV
Homology
BLAST of Spg014524 vs. NCBI nr
Match: AAO72728.1 (trichosanthin precursor [Trichosanthes kirilowii])

HSP 1 Score: 468.8 bits (1205), Expect = 3.5e-128
Identity = 246/287 (85.71%), Postives = 266/287 (92.68%), Query Frame = 0

Query: 4   LLLPLLLLAIFPRAPAVKGDVSFRLSGATTSSYGVFISNLRRALPNEKLLYNIPLLRSTL 63
           L+L LL+L +F   PAV+GDVSFRLSGAT+SSYGVFISNLR+ALPNE+ LY+IPLLRSTL
Sbjct: 5   LVLSLLILTLFLTTPAVEGDVSFRLSGATSSSYGVFISNLRKALPNERKLYDIPLLRSTL 64

Query: 64  PGAQRYTLIHLSNYADQTISVAVDVTNVYIMGYRAGDTSYFFNEASAAEAAKYVFKDARR 123
           PG+QRY LIHL+NYAD+TISVA+DVTNVYIMGYRAGDTSYFFNEASA EAAKYVFKDA R
Sbjct: 65  PGSQRYALIHLTNYADETISVAIDVTNVYIMGYRAGDTSYFFNEASATEAAKYVFKDAMR 124

Query: 124 KVTLPYSGNYERLQIAAGKIRENIPLGLPALDSAITTLFYYNANSAASALLVLIQSTAEA 183
           KVTLPYSGNYERLQ AAGKIRENIPLGLPALDSAITTLFYYNANSAASAL+VLIQST+EA
Sbjct: 125 KVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLFYYNANSAASALMVLIQSTSEA 184

Query: 184 ARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLINGNNE 243
           ARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLIN  N+
Sbjct: 185 ARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLINAQNQ 244

Query: 244 RVSITNVNAGVVTSNIALLLNRNNVVAAIDEDDVPRTLGFGYGGYAV 291
           RV+ITNV+AGVVTSNIALLLNRNN+ A   +DDVP T  FG G YA+
Sbjct: 245 RVTITNVDAGVVTSNIALLLNRNNMAAM--DDDVPMTQSFGCGSYAI 289

BLAST of Spg014524 vs. NCBI nr
Match: P09989.2 (RecName: Full=Ribosome-inactivating protein alpha-trichosanthin; Short=Alpha-TCS; AltName: Full=rRNA N-glycosidase; Flags: Precursor [Trichosanthes kirilowii] >AAA34207.1 trichosanthin precursor [Trichosanthes kirilowii])

HSP 1 Score: 467.2 bits (1201), Expect = 1.0e-127
Identity = 245/287 (85.37%), Postives = 266/287 (92.68%), Query Frame = 0

Query: 4   LLLPLLLLAIFPRAPAVKGDVSFRLSGATTSSYGVFISNLRRALPNEKLLYNIPLLRSTL 63
           L+L LL+L +F   PAV+GDVSFRLSGAT+SSYGVFISNLR+ALPNE+ LY+IPLLRS+L
Sbjct: 5   LVLSLLILTLFLTTPAVEGDVSFRLSGATSSSYGVFISNLRKALPNERKLYDIPLLRSSL 64

Query: 64  PGAQRYTLIHLSNYADQTISVAVDVTNVYIMGYRAGDTSYFFNEASAAEAAKYVFKDARR 123
           PG+QRY LIHL+NYAD+TISVA+DVTNVYIMGYRAGDTSYFFNEASA EAAKYVFKDA R
Sbjct: 65  PGSQRYALIHLTNYADETISVAIDVTNVYIMGYRAGDTSYFFNEASATEAAKYVFKDAMR 124

Query: 124 KVTLPYSGNYERLQIAAGKIRENIPLGLPALDSAITTLFYYNANSAASALLVLIQSTAEA 183
           KVTLPYSGNYERLQ AAGKIRENIPLGLPALDSAITTLFYYNANSAASAL+VLIQST+EA
Sbjct: 125 KVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLFYYNANSAASALMVLIQSTSEA 184

Query: 184 ARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLINGNNE 243
           ARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLIN  N+
Sbjct: 185 ARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLINAQNQ 244

Query: 244 RVSITNVNAGVVTSNIALLLNRNNVVAAIDEDDVPRTLGFGYGGYAV 291
           RV+ITNV+AGVVTSNIALLLNRNN+ A   +DDVP T  FG G YA+
Sbjct: 245 RVTITNVDAGVVTSNIALLLNRNNMAAM--DDDVPMTQSFGCGSYAI 289

BLAST of Spg014524 vs. NCBI nr
Match: P24478.2 (RecName: Full=Ribosome-inactivating protein karasurin-C; AltName: Full=rRNA N-glycosidase; Contains: RecName: Full=Ribosome-inactivating protein karasurin-A; Flags: Precursor [Trichosanthes kirilowii] >BAA21786.1 karasurin precursor [Trichosanthes kirilowii var. japonica])

HSP 1 Score: 466.1 bits (1198), Expect = 2.3e-127
Identity = 246/287 (85.71%), Postives = 268/287 (93.38%), Query Frame = 0

Query: 4   LLLPLLLLAIFPRAPAVKGDVSFRLSGATTSSYGVFISNLRRALPNEKLLYNIPLLRSTL 63
           L+  LL+L +F  APAV+GDVSFRLSGAT+SSYGVFISNLR+ALP E+ LY+IPLLRSTL
Sbjct: 5   LVFSLLILTLFLTAPAVEGDVSFRLSGATSSSYGVFISNLRKALPYERKLYDIPLLRSTL 64

Query: 64  PGAQRYTLIHLSNYADQTISVAVDVTNVYIMGYRAGDTSYFFNEASAAEAAKYVFKDARR 123
           PG+QRY LIHL+NYAD+TISVA+DVTNVY+MGYRAGDTSYFFNEASA EAAKYVFKDA+R
Sbjct: 65  PGSQRYALIHLTNYADETISVAIDVTNVYVMGYRAGDTSYFFNEASATEAAKYVFKDAKR 124

Query: 124 KVTLPYSGNYERLQIAAGKIRENIPLGLPALDSAITTLFYYNANSAASALLVLIQSTAEA 183
           KVTLPYSGNYERLQIAAGKIRENIPLGLPALDSAITTLFYYNANSAASAL+VLIQST+EA
Sbjct: 125 KVTLPYSGNYERLQIAAGKIRENIPLGLPALDSAITTLFYYNANSAASALMVLIQSTSEA 184

Query: 184 ARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLINGNNE 243
           ARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFE+PVVLIN  N+
Sbjct: 185 ARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFETPVVLINAQNQ 244

Query: 244 RVSITNVNAGVVTSNIALLLNRNNVVAAIDEDDVPRTLGFGYGGYAV 291
           RV+ITNV+AGVVTSNIALLLNRNN +AAID DDVP    FG G YA+
Sbjct: 245 RVTITNVDAGVVTSNIALLLNRNN-MAAID-DDVPMAQSFGCGSYAI 289

BLAST of Spg014524 vs. NCBI nr
Match: AAK52960.1 (trichosanthin precursor [Trichosanthes kirilowii])

HSP 1 Score: 465.7 bits (1197), Expect = 2.9e-127
Identity = 243/287 (84.67%), Postives = 266/287 (92.68%), Query Frame = 0

Query: 4   LLLPLLLLAIFPRAPAVKGDVSFRLSGATTSSYGVFISNLRRALPNEKLLYNIPLLRSTL 63
           L+L LL+L +F   PAV+GDVSFRLSGAT+SSYGVFISNLR+ALPNE+ LY+IPLLRS+L
Sbjct: 5   LVLSLLILTLFLTTPAVEGDVSFRLSGATSSSYGVFISNLRKALPNERKLYDIPLLRSSL 64

Query: 64  PGAQRYTLIHLSNYADQTISVAVDVTNVYIMGYRAGDTSYFFNEASAAEAAKYVFKDARR 123
           PG+QRY LIHL+NYAD+TISVA+DVTNVYIMGYRAGDTSYFFNEASA EAAKYVFKD+ R
Sbjct: 65  PGSQRYALIHLTNYADETISVAIDVTNVYIMGYRAGDTSYFFNEASATEAAKYVFKDSMR 124

Query: 124 KVTLPYSGNYERLQIAAGKIRENIPLGLPALDSAITTLFYYNANSAASALLVLIQSTAEA 183
           K+TLPYSGNYERLQ AAGKIRENIPLGLPALDSAITTLFYYNANSAASAL+VLIQST+EA
Sbjct: 125 KITLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLFYYNANSAASALMVLIQSTSEA 184

Query: 184 ARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLINGNNE 243
           ARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLIN  N+
Sbjct: 185 ARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLINAQNQ 244

Query: 244 RVSITNVNAGVVTSNIALLLNRNNVVAAIDEDDVPRTLGFGYGGYAV 291
           RV+ITNV+AGVVTSNIALLLNRNN+ A   +DDVP T  FG G YA+
Sbjct: 245 RVTITNVDAGVVTSNIALLLNRNNMAAM--DDDVPMTQSFGCGSYAI 289

BLAST of Spg014524 vs. NCBI nr
Match: AAA34206.1 (trichosanthin [Trichosanthes kirilowii])

HSP 1 Score: 465.3 bits (1196), Expect = 3.8e-127
Identity = 244/287 (85.02%), Postives = 265/287 (92.33%), Query Frame = 0

Query: 4   LLLPLLLLAIFPRAPAVKGDVSFRLSGATTSSYGVFISNLRRALPNEKLLYNIPLLRSTL 63
           L+L LL+L +F   PAV+GDVSFRLSGAT+SSYGVFISNLR+ALPNE+ LY+IPLLRS+L
Sbjct: 5   LVLSLLILTLFLTTPAVEGDVSFRLSGATSSSYGVFISNLRKALPNERKLYDIPLLRSSL 64

Query: 64  PGAQRYTLIHLSNYADQTISVAVDVTNVYIMGYRAGDTSYFFNEASAAEAAKYVFKDARR 123
           PG+QRY LIHL+NYAD+TISVA+DVTNVYIMGYRAGDTSYFFNEASA EAAKYVFKDA R
Sbjct: 65  PGSQRYALIHLTNYADETISVAIDVTNVYIMGYRAGDTSYFFNEASATEAAKYVFKDAMR 124

Query: 124 KVTLPYSGNYERLQIAAGKIRENIPLGLPALDSAITTLFYYNANSAASALLVLIQSTAEA 183
           KVTLPYSGNYERLQ AAGKIRENIPLGLPALDSAITTLFYYNANSAASAL+VLIQST+EA
Sbjct: 125 KVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLFYYNANSAASALMVLIQSTSEA 184

Query: 184 ARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLINGNNE 243
           ARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFE+PVVLIN  N+
Sbjct: 185 ARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFETPVVLINAQNQ 244

Query: 244 RVSITNVNAGVVTSNIALLLNRNNVVAAIDEDDVPRTLGFGYGGYAV 291
           RV ITNV+AGVVTSNIALLLNRNN+ A   +DDVP T  FG G YA+
Sbjct: 245 RVMITNVDAGVVTSNIALLLNRNNMAAM--DDDVPMTQSFGCGSYAI 289

BLAST of Spg014524 vs. ExPASy Swiss-Prot
Match: P09989 (Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 467.2 bits (1201), Expect = 1.3e-130
Identity = 245/287 (85.37%), Postives = 266/287 (92.68%), Query Frame = 0

Query: 4   LLLPLLLLAIFPRAPAVKGDVSFRLSGATTSSYGVFISNLRRALPNEKLLYNIPLLRSTL 63
           L+L LL+L +F   PAV+GDVSFRLSGAT+SSYGVFISNLR+ALPNE+ LY+IPLLRS+L
Sbjct: 5   LVLSLLILTLFLTTPAVEGDVSFRLSGATSSSYGVFISNLRKALPNERKLYDIPLLRSSL 64

Query: 64  PGAQRYTLIHLSNYADQTISVAVDVTNVYIMGYRAGDTSYFFNEASAAEAAKYVFKDARR 123
           PG+QRY LIHL+NYAD+TISVA+DVTNVYIMGYRAGDTSYFFNEASA EAAKYVFKDA R
Sbjct: 65  PGSQRYALIHLTNYADETISVAIDVTNVYIMGYRAGDTSYFFNEASATEAAKYVFKDAMR 124

Query: 124 KVTLPYSGNYERLQIAAGKIRENIPLGLPALDSAITTLFYYNANSAASALLVLIQSTAEA 183
           KVTLPYSGNYERLQ AAGKIRENIPLGLPALDSAITTLFYYNANSAASAL+VLIQST+EA
Sbjct: 125 KVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLFYYNANSAASALMVLIQSTSEA 184

Query: 184 ARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLINGNNE 243
           ARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLIN  N+
Sbjct: 185 ARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLINAQNQ 244

Query: 244 RVSITNVNAGVVTSNIALLLNRNNVVAAIDEDDVPRTLGFGYGGYAV 291
           RV+ITNV+AGVVTSNIALLLNRNN+ A   +DDVP T  FG G YA+
Sbjct: 245 RVTITNVDAGVVTSNIALLLNRNNMAAM--DDDVPMTQSFGCGSYAI 289

BLAST of Spg014524 vs. ExPASy Swiss-Prot
Match: P24478 (Ribosome-inactivating protein karasurin-C OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 466.1 bits (1198), Expect = 3.0e-130
Identity = 246/287 (85.71%), Postives = 268/287 (93.38%), Query Frame = 0

Query: 4   LLLPLLLLAIFPRAPAVKGDVSFRLSGATTSSYGVFISNLRRALPNEKLLYNIPLLRSTL 63
           L+  LL+L +F  APAV+GDVSFRLSGAT+SSYGVFISNLR+ALP E+ LY+IPLLRSTL
Sbjct: 5   LVFSLLILTLFLTAPAVEGDVSFRLSGATSSSYGVFISNLRKALPYERKLYDIPLLRSTL 64

Query: 64  PGAQRYTLIHLSNYADQTISVAVDVTNVYIMGYRAGDTSYFFNEASAAEAAKYVFKDARR 123
           PG+QRY LIHL+NYAD+TISVA+DVTNVY+MGYRAGDTSYFFNEASA EAAKYVFKDA+R
Sbjct: 65  PGSQRYALIHLTNYADETISVAIDVTNVYVMGYRAGDTSYFFNEASATEAAKYVFKDAKR 124

Query: 124 KVTLPYSGNYERLQIAAGKIRENIPLGLPALDSAITTLFYYNANSAASALLVLIQSTAEA 183
           KVTLPYSGNYERLQIAAGKIRENIPLGLPALDSAITTLFYYNANSAASAL+VLIQST+EA
Sbjct: 125 KVTLPYSGNYERLQIAAGKIRENIPLGLPALDSAITTLFYYNANSAASALMVLIQSTSEA 184

Query: 184 ARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLINGNNE 243
           ARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFE+PVVLIN  N+
Sbjct: 185 ARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFETPVVLINAQNQ 244

Query: 244 RVSITNVNAGVVTSNIALLLNRNNVVAAIDEDDVPRTLGFGYGGYAV 291
           RV+ITNV+AGVVTSNIALLLNRNN +AAID DDVP    FG G YA+
Sbjct: 245 RVTITNVDAGVVTSNIALLLNRNN-MAAID-DDVPMAQSFGCGSYAI 289

BLAST of Spg014524 vs. ExPASy Swiss-Prot
Match: P33185 (Ribosome-inactivating protein bryodin I OS=Bryonia dioica OX=3652 PE=1 SV=3)

HSP 1 Score: 436.0 bits (1120), Expect = 3.3e-121
Identity = 231/287 (80.49%), Postives = 259/287 (90.24%), Query Frame = 0

Query: 4   LLLPLLLLAIFPRAPAVKGDVSFRLSGATTSSYGVFISNLRRALPNEKLLYNIPLLRSTL 63
           L+L LL+L IF ++P V+GDVSFRLSGATT+SYGVFI NLR ALP E+ +YNIPLLRS++
Sbjct: 5   LVLWLLILTIFLKSPTVEGDVSFRLSGATTTSYGVFIKNLREALPYERKVYNIPLLRSSI 64

Query: 64  PGAQRYTLIHLSNYADQTISVAVDVTNVYIMGYRAGDTSYFFNEASAAEAAKYVFKDARR 123
            G+ RYTL+HL+NYAD+TISVAVDVTNVYIMGY AGD SYFFNEASA EAAK+VFKDA++
Sbjct: 65  SGSGRYTLLHLTNYADETISVAVDVTNVYIMGYLAGDVSYFFNEASATEAAKFVFKDAKK 124

Query: 124 KVTLPYSGNYERLQIAAGKIRENIPLGLPALDSAITTLFYYNANSAASALLVLIQSTAEA 183
           KVTLPYSGNYERLQ AAGKIRENIPLGLPALDSAITTL+YY A+SAASALLVLIQSTAE+
Sbjct: 125 KVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLYYYTASSAASALLVLIQSTAES 184

Query: 184 ARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLINGNNE 243
           ARYKFIEQQIGKRVDKTFLPSLA ISLEN+WSALSKQIQIASTNNGQFESPVVLI+GNN+
Sbjct: 185 ARYKFIEQQIGKRVDKTFLPSLATISLENNWSALSKQIQIASTNNGQFESPVVLIDGNNQ 244

Query: 244 RVSITNVNAGVVTSNIALLLNRNNVVAAIDEDDVPRTLGFGYGGYAV 291
           RVSITN +A VVTSNIALLLNRNN +AAI ED     +GF +G Y +
Sbjct: 245 RVSITNASARVVTSNIALLLNRNN-IAAIGEDISMTLIGFEHGLYGI 290

BLAST of Spg014524 vs. ExPASy Swiss-Prot
Match: P16094 (Ribosome-inactivating protein momordin I OS=Momordica charantia OX=3673 PE=1 SV=2)

HSP 1 Score: 331.3 bits (848), Expect = 1.1e-89
Identity = 181/279 (64.87%), Postives = 216/279 (77.42%), Query Frame = 0

Query: 5   LLPLLLLAIFPRAPAVKGDVSFRLSGATTSSYGVFISNLRRALPNEKLLYNIPLLRSTLP 64
           +L  L+LAIF     VKGDVSFRLSGA   SYG+FI +LR ALP  + +YNIPLL  ++ 
Sbjct: 6   VLSFLILAIFLGGSIVKGDVSFRLSGADPRSYGMFIKDLRNALPFREKVYNIPLLLPSVS 65

Query: 65  GAQRYTLIHLSNYADQTISVAVDVTNVYIMGYRAGDTSYFFNEASAAEAAKYVFKDARRK 124
           GA RY L+HL NY  +TI+VAVDVTNVYIMGY A  TSYFFNE +A  A++YVF+DARRK
Sbjct: 66  GAGRYLLMHLFNYDGKTITVAVDVTNVYIMGYLADTTSYFFNEPAAELASQYVFRDARRK 125

Query: 125 VTLPYSGNYERLQIAAGKIRENIPLGLPALDSAITTLFYYNANSAASALLVLIQSTAEAA 184
           +TLPYSGNYERLQIAAGK RE IP+GLPALDSAI+TL +Y++ +AA ALLVLIQ+TAEAA
Sbjct: 126 ITLPYSGNYERLQIAAGKPREKIPIGLPALDSAISTLLHYDSTAAAGALLVLIQTTAEAA 185

Query: 185 RYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLINGNNER 244
           R+K+IEQQI +R  +  +PSLA ISLENSWS LSKQIQ+A  NNG F +P+VL++    R
Sbjct: 186 RFKYIEQQIQERAYRDEVPSLATISLENSWSGLSKQIQLAQGNNGIFRTPIVLVDNKGNR 245

Query: 245 VSITNVNAGVVTSNIALLLNRNNVVAAIDEDDVPRTLGF 284
           V ITNV + VVTSNI LLLN  N +A  D  DV  T GF
Sbjct: 246 VQITNVTSKVVTSNIQLLLNTRN-IAEGDNGDVSTTHGF 283

BLAST of Spg014524 vs. ExPASy Swiss-Prot
Match: Q00465 (Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 7.9e-83
Identity = 167/268 (62.31%), Postives = 207/268 (77.24%), Query Frame = 0

Query: 8   LLLLAIFPRAPAVKGDVSFRLSGATTSSYGVFISNLRRALPNEKLLYNIPLLRSTLPGAQ 67
           +L+LAIF  A  V+ DV F LSG++++SY  FI +LR+ALP+   +YNI LL S+  GA 
Sbjct: 6   VLILAIFVAASTVEADVRFSLSGSSSTSYSKFIGDLRKALPSNGTVYNITLLLSSASGAS 65

Query: 68  RYTLIHLSNYADQTISVAVDVTNVYIMGYRAGDTSYFFNEASAAEAAKYVFKDARRKVTL 127
           RYTL+ LSNY  + I+VAVDVTNVYIMGY    TSYFFNE+ A  A++YVFK +   VTL
Sbjct: 66  RYTLMTLSNYDGKAITVAVDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGS-TIVTL 125

Query: 128 PYSGNYERLQIAAGKIRENIPLGLPALDSAITTLFYYNANSAASALLVLIQSTAEAARYK 187
           PYSGNYE+LQ AAGKIRE IPLG PALDSAITTLF+Y++ +AA+A LV+IQ+TAEA+R+K
Sbjct: 126 PYSGNYEKLQTAAGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAEASRFK 185

Query: 188 FIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLINGNNERVSI 247
           +IE QI +R+ K  +PSLA ISLEN WSALSKQIQ+A TNNG F++PVV+ +   +RV I
Sbjct: 186 YIEGQIIERISKNQVPSLATISLENEWSALSKQIQLAQTNNGTFKTPVVITDDKGQRVEI 245

Query: 248 TNVNAGVVTSNIALLLNRNNVVAAIDED 276
           TNV + VVT NI LLLN    VAA DED
Sbjct: 246 TNVTSKVVTKNIQLLLNYKQNVAAFDED 272

BLAST of Spg014524 vs. ExPASy TrEMBL
Match: Q84SV8 (rRNA N-glycosidase OS=Trichosanthes kirilowii OX=3677 GN=TCS PE=3 SV=1)

HSP 1 Score: 468.8 bits (1205), Expect = 1.7e-128
Identity = 246/287 (85.71%), Postives = 266/287 (92.68%), Query Frame = 0

Query: 4   LLLPLLLLAIFPRAPAVKGDVSFRLSGATTSSYGVFISNLRRALPNEKLLYNIPLLRSTL 63
           L+L LL+L +F   PAV+GDVSFRLSGAT+SSYGVFISNLR+ALPNE+ LY+IPLLRSTL
Sbjct: 5   LVLSLLILTLFLTTPAVEGDVSFRLSGATSSSYGVFISNLRKALPNERKLYDIPLLRSTL 64

Query: 64  PGAQRYTLIHLSNYADQTISVAVDVTNVYIMGYRAGDTSYFFNEASAAEAAKYVFKDARR 123
           PG+QRY LIHL+NYAD+TISVA+DVTNVYIMGYRAGDTSYFFNEASA EAAKYVFKDA R
Sbjct: 65  PGSQRYALIHLTNYADETISVAIDVTNVYIMGYRAGDTSYFFNEASATEAAKYVFKDAMR 124

Query: 124 KVTLPYSGNYERLQIAAGKIRENIPLGLPALDSAITTLFYYNANSAASALLVLIQSTAEA 183
           KVTLPYSGNYERLQ AAGKIRENIPLGLPALDSAITTLFYYNANSAASAL+VLIQST+EA
Sbjct: 125 KVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLFYYNANSAASALMVLIQSTSEA 184

Query: 184 ARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLINGNNE 243
           ARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLIN  N+
Sbjct: 185 ARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLINAQNQ 244

Query: 244 RVSITNVNAGVVTSNIALLLNRNNVVAAIDEDDVPRTLGFGYGGYAV 291
           RV+ITNV+AGVVTSNIALLLNRNN+ A   +DDVP T  FG G YA+
Sbjct: 245 RVTITNVDAGVVTSNIALLLNRNNMAAM--DDDVPMTQSFGCGSYAI 289

BLAST of Spg014524 vs. ExPASy TrEMBL
Match: Q94KE4 (rRNA N-glycosidase OS=Trichosanthes kirilowii OX=3677 GN=TCS PE=3 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 1.4e-127
Identity = 243/287 (84.67%), Postives = 266/287 (92.68%), Query Frame = 0

Query: 4   LLLPLLLLAIFPRAPAVKGDVSFRLSGATTSSYGVFISNLRRALPNEKLLYNIPLLRSTL 63
           L+L LL+L +F   PAV+GDVSFRLSGAT+SSYGVFISNLR+ALPNE+ LY+IPLLRS+L
Sbjct: 5   LVLSLLILTLFLTTPAVEGDVSFRLSGATSSSYGVFISNLRKALPNERKLYDIPLLRSSL 64

Query: 64  PGAQRYTLIHLSNYADQTISVAVDVTNVYIMGYRAGDTSYFFNEASAAEAAKYVFKDARR 123
           PG+QRY LIHL+NYAD+TISVA+DVTNVYIMGYRAGDTSYFFNEASA EAAKYVFKD+ R
Sbjct: 65  PGSQRYALIHLTNYADETISVAIDVTNVYIMGYRAGDTSYFFNEASATEAAKYVFKDSMR 124

Query: 124 KVTLPYSGNYERLQIAAGKIRENIPLGLPALDSAITTLFYYNANSAASALLVLIQSTAEA 183
           K+TLPYSGNYERLQ AAGKIRENIPLGLPALDSAITTLFYYNANSAASAL+VLIQST+EA
Sbjct: 125 KITLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLFYYNANSAASALMVLIQSTSEA 184

Query: 184 ARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLINGNNE 243
           ARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLIN  N+
Sbjct: 185 ARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLINAQNQ 244

Query: 244 RVSITNVNAGVVTSNIALLLNRNNVVAAIDEDDVPRTLGFGYGGYAV 291
           RV+ITNV+AGVVTSNIALLLNRNN+ A   +DDVP T  FG G YA+
Sbjct: 245 RVTITNVDAGVVTSNIALLLNRNNMAAM--DDDVPMTQSFGCGSYAI 289

BLAST of Spg014524 vs. ExPASy TrEMBL
Match: Q41216 (rRNA N-glycosidase OS=Trichosanthes kirilowii OX=3677 GN=trichosanthin: TCS PE=3 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 2.1e-126
Identity = 241/287 (83.97%), Postives = 266/287 (92.68%), Query Frame = 0

Query: 4   LLLPLLLLAIFPRAPAVKGDVSFRLSGATTSSYGVFISNLRRALPNEKLLYNIPLLRSTL 63
           L+L LL+L +F   PAV+GDVSFRLSGAT+SSYGVFISNLR+ALPNE+ LY+IPLLRS+L
Sbjct: 5   LVLSLLILTLFLTTPAVEGDVSFRLSGATSSSYGVFISNLRKALPNERKLYDIPLLRSSL 64

Query: 64  PGAQRYTLIHLSNYADQTISVAVDVTNVYIMGYRAGDTSYFFNEASAAEAAKYVFKDARR 123
           PG+QRY L+HL+NYAD+TISVA+DVT+VYIMGYRAGDTSYFFNEASA EAAKYVFKDA R
Sbjct: 65  PGSQRYALVHLTNYADETISVAIDVTSVYIMGYRAGDTSYFFNEASATEAAKYVFKDAMR 124

Query: 124 KVTLPYSGNYERLQIAAGKIRENIPLGLPALDSAITTLFYYNANSAASALLVLIQSTAEA 183
           KVTLPYSGNYERLQ AAGKIRENIPLGLPALDSAITTLFYYNANSAASAL+VLIQST+EA
Sbjct: 125 KVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLFYYNANSAASALMVLIQSTSEA 184

Query: 184 ARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLINGNNE 243
           ARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFE+PVVLIN  N+
Sbjct: 185 ARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFETPVVLINAQNQ 244

Query: 244 RVSITNVNAGVVTSNIALLLNRNNVVAAIDEDDVPRTLGFGYGGYAV 291
           RV+ITNV+AGVVTSNIALLLNRN++ A   +DDVP T  FG G YA+
Sbjct: 245 RVTITNVDAGVVTSNIALLLNRNDMAAM--DDDVPMTQSFGCGSYAI 289

BLAST of Spg014524 vs. ExPASy TrEMBL
Match: Q6PRG5 (rRNA N-glycosidase (Fragment) OS=Trichosanthes kirilowii OX=3677 GN=tcm PE=3 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 1.5e-121
Identity = 235/265 (88.68%), Postives = 252/265 (95.09%), Query Frame = 0

Query: 4   LLLPLLLLAIFPRAPAVKGDVSFRLSGATTSSYGVFISNLRRALPNEKLLYNIPLLRSTL 63
           L+L LL+L +F  APAV+GDVSFRLSGAT+SSYGVFISNLR+ALP EK LY+IPLLRS+L
Sbjct: 5   LVLSLLILTLFLTAPAVEGDVSFRLSGATSSSYGVFISNLRKALPYEKKLYDIPLLRSSL 64

Query: 64  PGAQRYTLIHLSNYADQTISVAVDVTNVYIMGYRAGDTSYFFNEASAAEAAKYVFKDARR 123
            G+QRY LI+L+NYAD+TISVA+DVTNVYIMGYRAGD SYFFNEASA EAAKYVFKDA+R
Sbjct: 65  SGSQRYALIYLTNYADETISVAIDVTNVYIMGYRAGDISYFFNEASATEAAKYVFKDAKR 124

Query: 124 KVTLPYSGNYERLQIAAGKIRENIPLGLPALDSAITTLFYYNANSAASALLVLIQSTAEA 183
           KVTLPYSGNYERLQIAAGKIRENIPLGLPALDSAITTLFYYNANSAASALLVLIQSTAEA
Sbjct: 125 KVTLPYSGNYERLQIAAGKIRENIPLGLPALDSAITTLFYYNANSAASALLVLIQSTAEA 184

Query: 184 ARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLINGNNE 243
           ARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLIN  N+
Sbjct: 185 ARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLINAQNQ 244

Query: 244 RVSITNVNAGVVTSNIALLLNRNNV 269
           RV+I NVNAGVVTSNIALLLNRNN+
Sbjct: 245 RVTIANVNAGVVTSNIALLLNRNNM 269

BLAST of Spg014524 vs. ExPASy TrEMBL
Match: Q8LPV7 (rRNA N-glycosidase (Fragment) OS=Trichosanthes kirilowii OX=3677 GN=TCS PE=3 SV=1)

HSP 1 Score: 444.1 bits (1141), Expect = 4.4e-121
Identity = 234/265 (88.30%), Postives = 251/265 (94.72%), Query Frame = 0

Query: 4   LLLPLLLLAIFPRAPAVKGDVSFRLSGATTSSYGVFISNLRRALPNEKLLYNIPLLRSTL 63
           L+L LL+L +F  APAV+GDVSFRLSGAT+SSYGVFISNLR+ALP EK LY+IPLLRS+L
Sbjct: 5   LVLSLLILTLFLTAPAVEGDVSFRLSGATSSSYGVFISNLRKALPYEKKLYDIPLLRSSL 64

Query: 64  PGAQRYTLIHLSNYADQTISVAVDVTNVYIMGYRAGDTSYFFNEASAAEAAKYVFKDARR 123
            G+QRY LI+L+NYAD+TISVA+DVTNVYIMGYRAGD SYFFNEASA EAAKYVFKDA+R
Sbjct: 65  SGSQRYALIYLTNYADETISVAIDVTNVYIMGYRAGDISYFFNEASATEAAKYVFKDAKR 124

Query: 124 KVTLPYSGNYERLQIAAGKIRENIPLGLPALDSAITTLFYYNANSAASALLVLIQSTAEA 183
           KVTLPYSGNYERLQIAAGKIRENIPLGLPALDSAITTLFYYNANSAASALLVLIQSTAEA
Sbjct: 125 KVTLPYSGNYERLQIAAGKIRENIPLGLPALDSAITTLFYYNANSAASALLVLIQSTAEA 184

Query: 184 ARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLINGNNE 243
           ARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLIN  N+
Sbjct: 185 ARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFESPVVLINAQNQ 244

Query: 244 RVSITNVNAGVVTSNIALLLNRNNV 269
           RV+I NVNAGVVTSNIA LLNRNN+
Sbjct: 245 RVTIANVNAGVVTSNIAFLLNRNNM 269

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AAO72728.13.5e-12885.71trichosanthin precursor [Trichosanthes kirilowii][more]
P09989.21.0e-12785.37RecName: Full=Ribosome-inactivating protein alpha-trichosanthin; Short=Alpha-TCS... [more]
P24478.22.3e-12785.71RecName: Full=Ribosome-inactivating protein karasurin-C; AltName: Full=rRNA N-gl... [more]
AAK52960.12.9e-12784.67trichosanthin precursor [Trichosanthes kirilowii][more]
AAA34206.13.8e-12785.02trichosanthin [Trichosanthes kirilowii][more]
Match NameE-valueIdentityDescription
P099891.3e-13085.37Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=... [more]
P244783.0e-13085.71Ribosome-inactivating protein karasurin-C OS=Trichosanthes kirilowii OX=3677 PE=... [more]
P331853.3e-12180.49Ribosome-inactivating protein bryodin I OS=Bryonia dioica OX=3652 PE=1 SV=3[more]
P160941.1e-8964.87Ribosome-inactivating protein momordin I OS=Momordica charantia OX=3673 PE=1 SV=... [more]
Q004657.9e-8362.31Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
Q84SV81.7e-12885.71rRNA N-glycosidase OS=Trichosanthes kirilowii OX=3677 GN=TCS PE=3 SV=1[more]
Q94KE41.4e-12784.67rRNA N-glycosidase OS=Trichosanthes kirilowii OX=3677 GN=TCS PE=3 SV=1[more]
Q412162.1e-12683.97rRNA N-glycosidase OS=Trichosanthes kirilowii OX=3677 GN=trichosanthin: TCS PE=3... [more]
Q6PRG51.5e-12188.68rRNA N-glycosidase (Fragment) OS=Trichosanthes kirilowii OX=3677 GN=tcm PE=3 SV=... [more]
Q8LPV74.4e-12188.30rRNA N-glycosidase (Fragment) OS=Trichosanthes kirilowii OX=3677 GN=TCS PE=3 SV=... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR017989Ribosome-inactivating protein type 1/2PRINTSPR00396SHIGARICINcoord: 34..47
score: 44.92
coord: 144..158
score: 58.77
coord: 173..193
score: 60.78
coord: 205..222
score: 53.65
coord: 81..96
score: 50.66
IPR016138Ribosome-inactivating protein, subdomain 1GENE3D3.40.420.10Ricin (A subunit), domain 1coord: 23..184
e-value: 1.8E-45
score: 156.6
IPR016139Ribosome-inactivating protein, subdomain 2GENE3D4.10.470.10Ricin (A Subunit), domain 2coord: 185..269
e-value: 9.1E-30
score: 104.5
IPR001574Ribosome-inactivating proteinPFAMPF00161RIPcoord: 32..222
e-value: 5.0E-31
score: 107.9
IPR001574Ribosome-inactivating proteinPANTHERPTHR33453FAMILY NOT NAMEDcoord: 26..264
NoneNo IPR availablePANTHERPTHR33453:SF14RRNA N-GLYCOSIDASEcoord: 26..264
IPR017988Ribosome-inactivating protein conserved sitePROSITEPS00275SHIGA_RICINcoord: 177..193
IPR036041Ribosome-inactivating protein superfamilySUPERFAMILY56371Ribosome inactivating proteins (RIP)coord: 23..267

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg014524.1Spg014524.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035821 modulation of process of other organism
biological_process GO:0017148 negative regulation of translation
biological_process GO:0050688 regulation of defense response to virus
molecular_function GO:0030598 rRNA N-glycosylase activity
molecular_function GO:0090729 toxin activity