Spg014523 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg014523
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionF-box/kelch-repeat protein SKIP30-like
Locationscaffold3: 43067877 .. 43069365 (+)
RNA-Seq ExpressionSpg014523
SyntenySpg014523
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGATATAGTCTCTATTTTAAGGTAACAAAGAATGCAAGACATTTACCAAACCATTTTTGTTTTAGTGCTTCAAAAGGAATAGGAGATTTGTGAAAAGGATTTGGTAGAATTCTGGTTACTTTTGCCCCATCACATTGGCTGGTTTCTCATATTAGACTTTTCCAATAGACCTGGAACGAGGTTTCATCTATCAAAACTTGAATATTTTCTCAGATGTAAATTAAGTTTTCCTATTTGGAAGCTTAGTTATAAGTGTCTATTGCCTCAATCATCACAGTGGCCTAGGAATTCATTTTATTTTGCTCTTTATGATATCTTAGTTCCTTCGTATGATCTTTTTGGAACTAGAAGAGGGTGCTGATTTAATTTTATTCACCTCATATTTTTGTATGTTTCTTGATGTTTCGATTAGCTAGCTGCGCTGTGTGGAGATAATCTGGAATTGAAGATCATGTCTAGTCTGATTGAAGGTCTCCCTAATGAAGTTGCCCTTCGATGCCTCGCTTTTGTTCCCTTTTTCCTTCTCCCTAAGTTGGAACTCGTTTCTCGCTCGTGGAAAGCTGCCGTTCGTAGTGTTGAACTGTTCAAAGCTCGACAGGAGGTTGGTTCATTGGTGGATTTACTATGTGTATGCTATTACGATAAAGATAATGCATGGCAGCTTTATGACCCTGTTCAAGACCTTTGGATCACCCTCCCTCGACTTCCAACGACAAGAAAGCAACATTTTGGTGTCGTCTCCACTTCTCAAAAGCTGTTTGTTCTTGGTGGTTACAACTTAATTCTTGATCCATTGACTCGTGTTCTCGACGACGATGATATATGCAACGAGGTATGGTCCTTTGACCCCGTAACTCGCAATTGGTCGATGCGAGCACCAATGCTTGAGGCACGCGCACAGTTTGCTTGCTGTGTTTTGGATGGGAAGATCATTGTTGCGGGTGGCATCACTGGAAAACGTGAATCAACTTCTAAGGTAGAAATGTATGACTCTGTGAAGGATCTTTGGGTTCCATTACCTGATCTACCTGAAGCCAATGATTCAGGGACTGCATTAATGGTTGGAAGAAAAATGCATGTATTCTCCAATGGCCCCATAGTGCATACATTTGACAGTCTGGAGTGTAGGTGGAGTGTTGACACTGTTAAGATTAAGGATTACAGAAGGTTTTACCTCGACTGCGTGGCTGTTGCTCAAGATTCACTCTACATCATGACCAGTGAATACATCTTCAAACAGGATGGACAAGACTACAAAGTTTTTGTTTTGGCAAGTCAATTTCATGGCAGATCTAGGATGGCGATGATCTATTTTGGAGGCAAACTGTATGTGATTGGAGGATTACTTCACCACCCGAGCTATGCTGAACATCTGTCTGACGTGCACGTTCTTGCACTGGATAGCACGAGGCCAACTTGGTTTAATGCTGCTCCTATGAGTCGAGGATGTGGAGAGGTTCTTGGTTGTGCAGTCTTGAAACTTTAA

mRNA sequence

ATGAGATATAGTCTCTATTTTAAGCTAGCTGCGCTGTGTGGAGATAATCTGGAATTGAAGATCATGTCTAGTCTGATTGAAGGTCTCCCTAATGAAGTTGCCCTTCGATGCCTCGCTTTTGTTCCCTTTTTCCTTCTCCCTAAGTTGGAACTCGTTTCTCGCTCGTGGAAAGCTGCCGTTCGTAGTGTTGAACTGTTCAAAGCTCGACAGGAGGTTGGTTCATTGGTGGATTTACTATGTGTATGCTATTACGATAAAGATAATGCATGGCAGCTTTATGACCCTGTTCAAGACCTTTGGATCACCCTCCCTCGACTTCCAACGACAAGAAAGCAACATTTTGGTGTCGTCTCCACTTCTCAAAAGCTGTTTGTTCTTGGTGGTTACAACTTAATTCTTGATCCATTGACTCGTGTTCTCGACGACGATGATATATGCAACGAGGTATGGTCCTTTGACCCCGTAACTCGCAATTGGTCGATGCGAGCACCAATGCTTGAGGCACGCGCACAGTTTGCTTGCTGTGTTTTGGATGGGAAGATCATTGTTGCGGGTGGCATCACTGGAAAACGTGAATCAACTTCTAAGGTAGAAATGTATGACTCTGTGAAGGATCTTTGGGTTCCATTACCTGATCTACCTGAAGCCAATGATTCAGGGACTGCATTAATGGTTGGAAGAAAAATGCATGTATTCTCCAATGGCCCCATAGTGCATACATTTGACAGTCTGGAGTGTAGGTGGAGTGTTGACACTGTTAAGATTAAGGATTACAGAAGGTTTTACCTCGACTGCGTGGCTGTTGCTCAAGATTCACTCTACATCATGACCAGTGAATACATCTTCAAACAGGATGGACAAGACTACAAAGTTTTTGTTTTGGCAAGTCAATTTCATGGCAGATCTAGGATGGCGATGATCTATTTTGGAGGCAAACTGTATGTGATTGGAGGATTACTTCACCACCCGAGCTATGCTGAACATCTGTCTGACGTGCACGTTCTTGCACTGGATAGCACGAGGCCAACTTGGTTTAATGCTGCTCCTATGAGTCGAGGATGTGGAGAGGTTCTTGGTTGTGCAGTCTTGAAACTTTAA

Coding sequence (CDS)

ATGAGATATAGTCTCTATTTTAAGCTAGCTGCGCTGTGTGGAGATAATCTGGAATTGAAGATCATGTCTAGTCTGATTGAAGGTCTCCCTAATGAAGTTGCCCTTCGATGCCTCGCTTTTGTTCCCTTTTTCCTTCTCCCTAAGTTGGAACTCGTTTCTCGCTCGTGGAAAGCTGCCGTTCGTAGTGTTGAACTGTTCAAAGCTCGACAGGAGGTTGGTTCATTGGTGGATTTACTATGTGTATGCTATTACGATAAAGATAATGCATGGCAGCTTTATGACCCTGTTCAAGACCTTTGGATCACCCTCCCTCGACTTCCAACGACAAGAAAGCAACATTTTGGTGTCGTCTCCACTTCTCAAAAGCTGTTTGTTCTTGGTGGTTACAACTTAATTCTTGATCCATTGACTCGTGTTCTCGACGACGATGATATATGCAACGAGGTATGGTCCTTTGACCCCGTAACTCGCAATTGGTCGATGCGAGCACCAATGCTTGAGGCACGCGCACAGTTTGCTTGCTGTGTTTTGGATGGGAAGATCATTGTTGCGGGTGGCATCACTGGAAAACGTGAATCAACTTCTAAGGTAGAAATGTATGACTCTGTGAAGGATCTTTGGGTTCCATTACCTGATCTACCTGAAGCCAATGATTCAGGGACTGCATTAATGGTTGGAAGAAAAATGCATGTATTCTCCAATGGCCCCATAGTGCATACATTTGACAGTCTGGAGTGTAGGTGGAGTGTTGACACTGTTAAGATTAAGGATTACAGAAGGTTTTACCTCGACTGCGTGGCTGTTGCTCAAGATTCACTCTACATCATGACCAGTGAATACATCTTCAAACAGGATGGACAAGACTACAAAGTTTTTGTTTTGGCAAGTCAATTTCATGGCAGATCTAGGATGGCGATGATCTATTTTGGAGGCAAACTGTATGTGATTGGAGGATTACTTCACCACCCGAGCTATGCTGAACATCTGTCTGACGTGCACGTTCTTGCACTGGATAGCACGAGGCCAACTTGGTTTAATGCTGCTCCTATGAGTCGAGGATGTGGAGAGGTTCTTGGTTGTGCAGTCTTGAAACTTTAA

Protein sequence

MRYSLYFKLAALCGDNLELKIMSSLIEGLPNEVALRCLAFVPFFLLPKLELVSRSWKAAVRSVELFKARQEVGSLVDLLCVCYYDKDNAWQLYDPVQDLWITLPRLPTTRKQHFGVVSTSQKLFVLGGYNLILDPLTRVLDDDDICNEVWSFDPVTRNWSMRAPMLEARAQFACCVLDGKIIVAGGITGKRESTSKVEMYDSVKDLWVPLPDLPEANDSGTALMVGRKMHVFSNGPIVHTFDSLECRWSVDTVKIKDYRRFYLDCVAVAQDSLYIMTSEYIFKQDGQDYKVFVLASQFHGRSRMAMIYFGGKLYVIGGLLHHPSYAEHLSDVHVLALDSTRPTWFNAAPMSRGCGEVLGCAVLKL
Homology
BLAST of Spg014523 vs. NCBI nr
Match: KAG7014455.1 (F-box/kelch-repeat protein SKIP30 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 390.6 bits (1002), Expect = 1.5e-104
Identity = 202/356 (56.74%), Postives = 251/356 (70.51%), Query Frame = 0

Query: 12  LCGDNLELKIMSSLIEGLPNEVALRCLAFVPFFLLPKLELVSRSWKAAVRSVELFKARQE 71
           LC +  E KIMS LIEGLP+ +++RCLAFVP++L PKLELVSRSWKAA+RS ELF+ARQE
Sbjct: 53  LCTNEYERKIMSGLIEGLPDAISIRCLAFVPYYLHPKLELVSRSWKAAIRSTELFRARQE 112

Query: 72  VGSLVDLLCVCYYDKDNAWQLYDPVQDLWITLPRLPTTRKQ--HFGVVSTSQKLFVLGGY 131
           VGS  DLLCVC YD +N WQLYDP+Q+LW+TLP LP+  +   +FG VSTSQKLFVLGG 
Sbjct: 113 VGSSEDLLCVCSYDPNNTWQLYDPLQNLWMTLPELPSKIRHLANFGAVSTSQKLFVLGGR 172

Query: 132 NLILDPLTRVLDDDDICNEVWSFDPVTRNWSMRAPMLEARAQFACCVLDGKIIVAGGITG 191
              +DP+T   DD+   NEVWSFDPVTR WSMRAPML  R+ FACCV+DGKI+VAGG T 
Sbjct: 173 GDAVDPVTGDRDDNSSTNEVWSFDPVTRTWSMRAPMLVPRSTFACCVVDGKIVVAGGFTS 232

Query: 192 KRESTSKVEMYDSVKDLWVPLPDLPEANDSG-TALMVGRKMHVFSNG-PIVHTFDSLECR 251
             +STS+ EMYDS KD+W+PLPDL   +DS    LM+G +MH+   G   V  FDSLE +
Sbjct: 233 LSKSTSQAEMYDSEKDVWIPLPDLLHTHDSTCIGLMIGGRMHIMYGGVSTVQVFDSLELK 292

Query: 252 WSVDTVKIKDYRRFYLDCVAVAQDSLYIMTSEYIFKQDGQDYKVFVLASQFHGRSRMAMI 311
           W     +++DY  +     AV   SLY M+   +FKQ+ +D +  V ASQF  R  MAM+
Sbjct: 293 W-----RVEDY-GWLPGHKAVVGGSLYTMSQGLVFKQEERDAQAVVSASQFVQRIGMAMV 352

Query: 312 YFGGKLYVIGGLLHHPSY---AEHLSDVHVLALDSTRPTWFNAAPMSRGCGEVLGC 361
            F G+LYVIGG++    +      LSDVHVL + + +P+W  AAPMSR  G VLGC
Sbjct: 353 GFRGELYVIGGVVRPERFNGDLVKLSDVHVLQVRNEKPSWSCAAPMSRCSGTVLGC 402

BLAST of Spg014523 vs. NCBI nr
Match: XP_022150165.1 (F-box/kelch-repeat protein SKIP30-like [Momordica charantia])

HSP 1 Score: 390.2 bits (1001), Expect = 2.0e-104
Identity = 208/352 (59.09%), Postives = 250/352 (71.02%), Query Frame = 0

Query: 22  MSSLIEGLPNEVALRCLAFVPFFLLPKLELVSRSWKAAVRSVELFKARQEVGSLVDLLCV 81
           MS LIEGLP+ +ALRCLA VP++L PKLELVSRSWKAA RS ELF+ARQEVGS  DLLCV
Sbjct: 1   MSGLIEGLPDAIALRCLARVPYYLHPKLELVSRSWKAAFRSAELFRARQEVGSSEDLLCV 60

Query: 82  CYYDKDNAWQLYDPVQDLWITLPRLPTTRK--QHFGVVSTSQKLFVLGGYNLILDPLTRV 141
           C YD +N WQLYDP++DLWITLP LP+ R+   HFG  STSQ LFVLGG +  +DPLT  
Sbjct: 61  CSYDPNNTWQLYDPLRDLWITLPELPSKRRHLSHFGAASTSQYLFVLGGRSDAIDPLTGD 120

Query: 142 LDDDDICNEVWSFDPVTRNWSMRAPMLEARAQFACCVLDGKIIVAGGITGKRESTSKVEM 201
            DD+   NEVWSFDPVTRNW MRAPML  RA FACCVL+GKIIVAGG T   +STSK E+
Sbjct: 121 HDDNLSTNEVWSFDPVTRNWLMRAPMLVPRAMFACCVLEGKIIVAGGFTSTSKSTSKTEI 180

Query: 202 YDSVKDLWVPLPDLPEANDSG-TALMVGRKMHVFSNG-PIVHTFDSLECRWSVDTVKIKD 261
           YDS KDLWVPLPDL + +DS    +++G K+H+   G   V  FD+LE +W     +++D
Sbjct: 181 YDSEKDLWVPLPDLLQTHDSTCIGVVIGGKVHIMYKGQSTVQIFDTLELKW-----RVED 240

Query: 262 YRRFYLDCVAVAQDSLYIMTSEYIFKQDGQDYKVFVLA-SQFHGRSRMAMIYFGGKLYVI 321
           Y  +    +A  QDSLY+M    IFKQDG+D +V + A SQF  R  MAMI F G+LYVI
Sbjct: 241 Y-GWLPGPMAAVQDSLYVMCLGLIFKQDGRDRQVVIPAVSQFLHRIGMAMIGFRGELYVI 300

Query: 322 GGLLHH---PSYAEHLSDVHVLALDSTRPTWFNAAPMSRGCGEVLGCAVLKL 366
           GG++      +    LSDVHVL L   +PTW+  APMSR  G VLGC  L++
Sbjct: 301 GGVVRPVRLDADLVKLSDVHVLNLRGEKPTWYCVAPMSRCHGTVLGCTELRI 346

BLAST of Spg014523 vs. NCBI nr
Match: XP_011659773.1 (F-box/kelch-repeat protein SKIP30 [Cucumis sativus])

HSP 1 Score: 385.2 bits (988), Expect = 6.3e-103
Identity = 197/356 (55.34%), Postives = 249/356 (69.94%), Query Frame = 0

Query: 15  DNLELKIMSSLIEGLPNEVALRCLAFVPFFLLPKLELVSRSWKAAVRSVELFKARQEVGS 74
           D+LEL IMSSLIEGLP++VALRCLAFVPF+L   LE V  SW+ A+ S E++K R E G+
Sbjct: 33  DSLELNIMSSLIEGLPHDVALRCLAFVPFYLHATLEQVCHSWRDAICSGEIYKVRSECGT 92

Query: 75  LVDLLCVCYYDKDNAWQLYDPVQDLWITLPRLPTTRKQHFGVVSTSQKLFVLGGYNLILD 134
             DLL VC +D++N WQ YDP+++ W+TLP LP  RK +FGVVST QKLF+LGG  L+++
Sbjct: 93  AEDLLFVCCHDEENKWQFYDPIENFWVTLPELPGGRKHYFGVVSTHQKLFILGG--LLIN 152

Query: 135 PLTRVLDDDDICNEVWSFDPVTRNWSMRAPMLEARAQFACCVLDGKIIVAGGITGKREST 194
            +   +D+D  CNEVWSF+P+TR WS++APM EAR+ FAC +LDG IIV GG+  K EST
Sbjct: 153 AIDPSIDEDFSCNEVWSFNPMTRKWSIQAPMHEARSLFACGILDGMIIVVGGMNKKFEST 212

Query: 195 SKVEMYDSVKDLWVPLPDLPEANDSGTAL--MVGRKMHVFSNG-PIVHTFDSLECRWSVD 254
            K EMYD VKD+W+ LPDLP   DSG  +  +VGRKMH    G PIV TFD++E RW+  
Sbjct: 213 PKAEMYDPVKDVWIQLPDLPRICDSGICMGVVVGRKMHFIYKGLPIVQTFDTVEWRWT-- 272

Query: 255 TVKIKDYRRF-YLDCVAVAQDSLYIMTSEYIFKQDGQDYKVFVLASQFHGRSRMAMIYFG 314
              I+DY  F ++  +   +D +YIM+  YIF Q GQD KV + A QF+    M MI F 
Sbjct: 273 ---IEDYNWFSHIWLMTADRDRIYIMSQGYIFLQIGQDSKVVISADQFNLNDGMGMICFR 332

Query: 315 GKLYVIGGLLHHPSYAEHLSDVHVLALDST-RPTWFNAAPMSRGCGEVLGCAVLKL 366
           G+LYVIGG L+     E+LSDVHVL L S  R  W   APMSRG G VLGCA L++
Sbjct: 333 GELYVIGGTLYTDRDYEYLSDVHVLTLSSDFRTCWITIAPMSRGYGSVLGCAALRV 381

BLAST of Spg014523 vs. NCBI nr
Match: XP_022953500.1 (F-box/kelch-repeat protein SKIP30-like [Cucurbita moschata] >XP_022953501.1 F-box/kelch-repeat protein SKIP30-like [Cucurbita moschata])

HSP 1 Score: 385.2 bits (988), Expect = 6.3e-103
Identity = 199/351 (56.70%), Postives = 249/351 (70.94%), Query Frame = 0

Query: 22  MSSLIEGLPNEVALRCLAFVPFFLLPKLELVSRSWKAAVRSVELFKARQEVGSLVDLLCV 81
           MS LIEGLP+ +++RCLAFVP++L PKLELVSRSWKAA+RS ELF+ARQEVGS  DLLCV
Sbjct: 1   MSGLIEGLPDAISIRCLAFVPYYLHPKLELVSRSWKAAIRSTELFRARQEVGSSEDLLCV 60

Query: 82  CYYDKDNAWQLYDPVQDLWITLPRLPT--TRKQHFGVVSTSQKLFVLGGYNLILDPLTRV 141
           C YD +N WQLYDP+Q+LW+TLP LP+  T   +FGVVSTSQKLFVLGG    +DP+T  
Sbjct: 61  CSYDPNNTWQLYDPLQNLWMTLPELPSKITHLANFGVVSTSQKLFVLGGRGDAVDPVTGD 120

Query: 142 LDDDDICNEVWSFDPVTRNWSMRAPMLEARAQFACCVLDGKIIVAGGITGKRESTSKVEM 201
            +D+   NEVWSFDPVTR WSMRAPML  R  FACCV+DGKI+VAGG T   +STS+ EM
Sbjct: 121 REDNSSTNEVWSFDPVTRTWSMRAPMLVPRYTFACCVVDGKIVVAGGFTSLSKSTSQAEM 180

Query: 202 YDSVKDLWVPLPDLPEANDSG-TALMVGRKMHVFSNG-PIVHTFDSLECRWSVDTVKIKD 261
           YD+ KD+W+PLPDL   +DS    LM+G KMHV   G   V  FDSLE +W     +++D
Sbjct: 181 YDNEKDVWIPLPDLLHTHDSTCIGLMIGGKMHVMYGGVSTVQVFDSLELKW-----RVED 240

Query: 262 YRRFYLDCVAVAQDSLYIMTSEYIFKQDGQDYKVFVLASQFHGRSRMAMIYFGGKLYVIG 321
           Y  +     AV   SLY M+   +FKQ+ +D +  V ASQF  R  MA++ F G+LYVIG
Sbjct: 241 Y-GWLPGHKAVVGGSLYTMSQGLVFKQEERDAQAVVSASQFVQRIGMAIVGFRGELYVIG 300

Query: 322 GLLHHPSY---AEHLSDVHVLALDSTRPTWFNAAPMSRGCGEVLGCAVLKL 366
           G++    +      LSDVHVL + + +P+W+ AAPMSR  G VLGC V ++
Sbjct: 301 GVVRPERFDGDLVKLSDVHVLKVRNEKPSWYCAAPMSRCSGTVLGCTVWRI 345

BLAST of Spg014523 vs. NCBI nr
Match: XP_038896848.1 (F-box/kelch-repeat protein SKIP30-like [Benincasa hispida] >XP_038896849.1 F-box/kelch-repeat protein SKIP30-like [Benincasa hispida])

HSP 1 Score: 384.8 bits (987), Expect = 8.3e-103
Identity = 196/349 (56.16%), Postives = 249/349 (71.35%), Query Frame = 0

Query: 22  MSSLIEGLPNEVALRCLAFVPFFLLPKLELVSRSWKAAVRSVELFKARQEVGSLVDLLCV 81
           MSSLIEGLP++VALRCLAFVPF+L P LELV RSW+  + S E+++ R E G++ DLL V
Sbjct: 1   MSSLIEGLPHDVALRCLAFVPFYLHPTLELVCRSWRDVICSGEIYRVRWECGTVEDLLFV 60

Query: 82  CYYDKDNAWQLYDPVQDLWITLPRLPTTRKQHFGVVSTSQKLFVLGGYNLILDPLTRVLD 141
           C +D++N WQ YDP++D W+TLP LP  RK +FGVVST QKLF+LGG  L+++ +   +D
Sbjct: 61  CCHDEENKWQFYDPIEDFWVTLPELPGGRKHYFGVVSTYQKLFILGG--LLINTIDPSID 120

Query: 142 DDDICNEVWSFDPVTRNWSMRAPMLEARAQFACCVLDGKIIVAGGITGKRESTSKVEMYD 201
           +D  C EVWSF+P+TR W  +APM E+RA FAC VLDGKIIVAGG+  K ESTSK EMYD
Sbjct: 121 EDFSCTEVWSFNPMTRKWKQQAPMHESRALFACGVLDGKIIVAGGMNRKFESTSKAEMYD 180

Query: 202 SVKDLWVPLPDLPEANDSGTAL--MVGRKMHVFSNG-PIVHTFDSLECRWSVDTVKIKDY 261
            VKD+W+ LPDLP++ DSG  +  +VGRK+H    G PIV TFDS+E RW+V+     DY
Sbjct: 181 PVKDVWIELPDLPQSCDSGICMGVVVGRKLHFIYKGLPIVQTFDSVEWRWTVE-----DY 240

Query: 262 RRF-YLDCVAVAQDSLYIMTSEYIFKQDGQDYKVFVLASQFHGRSRMAMIYFGGKLYVIG 321
             F ++  +   +D +YIM+  YIF Q GQ+ KV + A QF+    M MI F G+LYVIG
Sbjct: 241 NWFSHIWLMTADRDVIYIMSQGYIFMQVGQNSKVVISADQFNLNDGMGMICFRGELYVIG 300

Query: 322 GLLHHPSYAEHLSDVHVLALDST-RPTWFNAAPMSRGCGEVLGCAVLKL 366
           G L+     E+LSDVHVL L S  R  W N APMSRG G VLGCA L++
Sbjct: 301 GTLYTDRDYEYLSDVHVLTLCSNLRTCWVNTAPMSRGYGTVLGCAALRV 342

BLAST of Spg014523 vs. ExPASy Swiss-Prot
Match: Q9M1W7 (F-box/kelch-repeat protein SKIP30 OS=Arabidopsis thaliana OX=3702 GN=SKIP30 PE=1 SV=2)

HSP 1 Score: 289.3 bits (739), Expect = 6.2e-77
Identity = 155/351 (44.16%), Postives = 214/351 (60.97%), Query Frame = 0

Query: 22  MSSLIEGLPNEVALRCLAFVPFFLLPKLELVSRSWKAAVRSVELFKARQEVGSLVDLLCV 81
           MS L++G+P  VALRCLA VP  L P LELVSRSW+AA+RS ELF+ R+E+ S   LLCV
Sbjct: 8   MSGLLDGIPEAVALRCLAHVPLHLHPNLELVSRSWRAAIRSHELFRVRKELRSSEHLLCV 67

Query: 82  CYYDKDNAWQLYDPVQDLWITLPRLPTTRKQ--HFGVVSTSQKLFVLGGYNLILDPLTRV 141
           C +D +N WQ+Y P  D W+TLP LP+  +   HFG V+T+  LFVLGG +  + P+T  
Sbjct: 68  CAFDPENIWQVYSPNCDRWLTLPLLPSRIRHLAHFGAVTTAGMLFVLGGGSDAVSPVTGD 127

Query: 142 LDDDDICNEVWSFDPVTRNWSMRAPMLEARAQFACCVLDGKIIVAGGITGKRESTSKVEM 201
            D     ++VWS+D V R W+ RA ML  RA FACCVL GKI+VAGG T  R+S S  EM
Sbjct: 128 HDGTFATDQVWSYDFVQRQWTPRASMLVPRAMFACCVLQGKIVVAGGFTTCRKSISGAEM 187

Query: 202 YDSVKDLWVPLPDLPEANDSG-TALMVGRKMHVFSNG-PIVHTFDSLECRWSVDTVKIKD 261
           YD   D+W  +PDL + ++S  + L+V  K+HV   G   V   +S++  W V     KD
Sbjct: 188 YDPENDVWTSIPDLHQTHNSACSGLVVNGKVHVLHKGLSTVQVLESVKLGWDV-----KD 247

Query: 262 YRRFYLDCVAVAQDSLYIMTSEYIFKQDGQDYKVFVLASQFHGRSRMAMIYFGGKLYVIG 321
           Y  +    + V +D LY+M+   +FKQ+G  +K+   AS+F  R  MAM     ++ ++G
Sbjct: 248 Y-GWPQGPMVVVEDVLYVMSHGLVFKQEGDTWKMVASASEFKRRIGMAMTSLSDEVLIVG 307

Query: 322 GLLHHPSY---AEHLSDVHVLALDSTRPTWFNAAPMSRGCGEVLGCAVLKL 366
           G++         + LSDV  L + + RP W + APM+R  G +LGC  L +
Sbjct: 308 GVIGPDRLNWDIKPLSDVDALTVGNDRPAWRSVAPMTRCRGTILGCTQLTI 352

BLAST of Spg014523 vs. ExPASy Swiss-Prot
Match: Q0WW40 (F-box/kelch-repeat protein At1g16250 OS=Arabidopsis thaliana OX=3702 GN=At1g16250 PE=2 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 7.4e-22
Identity = 74/221 (33.48%), Postives = 96/221 (43.44%), Query Frame = 0

Query: 21  IMSSLIEGLPNEVALRCLAFVPFFLLPKLELVSRSWKAAVRSVELFKARQEVGSLVDLLC 80
           I  S+I GLP+++ALRC+A +       LE VSR W+  VR  +    +   G     L 
Sbjct: 4   IEQSIIPGLPDDLALRCIAKLSHGYHGVLECVSRGWRDLVRGADYSCYKARNGWSGSWLF 63

Query: 81  VCYYDKDNAWQLYDPVQDLWITLPRLPTTR----KQHFGVVSTSQKLFVLGG-YNLILDP 140
           V      N W  YDP  D W  LPR    +       F  V  S  L V+GG Y   +  
Sbjct: 64  VLTERSKNQWVAYDPEADRWHPLPRTRAVQDGWHHSGFACVCVSNCLLVIGGCYAPSVSS 123

Query: 141 LTRVLDDDDICNEVWSFDPVTRNWSMRAPMLEARAQFACCVLDGKIIVAGG--ITGKRES 200
                    +  +V  FDP  + W M A M   R  FAC  + GK+ VAGG  +T  R  
Sbjct: 124 FPH--QKPVVTKDVMRFDPFKKQWKMVASMRTPRTHFACTSVSGKVYVAGGRNLTHSRGI 183

Query: 201 TSKVEMYDSVKDLWVPLPDLPEANDSGTALMVGRKMHVFSN 235
            S  E+YD V D W  LP +P      + L      HV S+
Sbjct: 184 PS-AEVYDPVADRWEELPAMPRPQMDCSGLSYRGCFHVLSD 221

BLAST of Spg014523 vs. ExPASy Swiss-Prot
Match: Q9CAG8 (F-box/kelch-repeat protein At1g67480 OS=Arabidopsis thaliana OX=3702 GN=At1g67480 PE=2 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 2.2e-21
Identity = 70/235 (29.79%), Postives = 103/235 (43.83%), Query Frame = 0

Query: 25  LIEGLPNEVALRCLAFVPFFLLPKLELVSRSWKAAVRSVELFKARQEVGSLVDLLCVCYY 84
           LI GLP++VA +CLA VP    P +  V + W+  V+S E    R+  G L + L V   
Sbjct: 39  LIPGLPDDVAKQCLALVPRARFPSMGSVCKKWRFVVQSKEFITVRRLAGMLEEWLYVLTM 98

Query: 85  D---KDNAWQLYDPVQDLWITLPRLPTTRKQHFGVVSTSQKLFVLGGYNLILDPLTRVLD 144
           +   KDN W++ D +     +LP +P   K  F VV    KL V+ G  +I   L    D
Sbjct: 99  NAGGKDNRWEVMDCLGQKLSSLPPMPGPAKTGFKVVVVDGKLLVIAGCCMINGSLVASAD 158

Query: 145 DDDICNEVWSFDPVTRNWSMRAPMLEARAQFACCVLDGKIIVAGGITGKRESTSKVEMYD 204
                  V+ +D    +WS  A +  AR  FAC  ++G + V GG     ES S  E+YD
Sbjct: 159 -------VYQYDTCLNSWSRLADLEVARYDFACAEVNGHVYVVGGHGVDGESLSSAEVYD 218

Query: 205 SVKDLWVPLPDL--------PEANDSGTALMVGRKMHVFSNGPIVHTFDSLECRW 249
                W  +  L          A +    +M GR      N  ++  +++    W
Sbjct: 219 PETCTWTFIESLRRPRWGCFASAFNGKLYVMGGRSNFTIGNSKLLDVYNTQCGSW 266

BLAST of Spg014523 vs. ExPASy Swiss-Prot
Match: Q8CA72 (Gigaxonin OS=Mus musculus OX=10090 GN=Gan PE=1 SV=2)

HSP 1 Score: 94.4 bits (233), Expect = 2.9e-18
Identity = 81/300 (27.00%), Postives = 119/300 (39.67%), Query Frame = 0

Query: 92  LYDPVQDLWITLPRLPTTRKQHFGVVSTSQKLFVLGGYNLILDPLTRVLDDDDICNEVWS 151
           LYDP + LWI L  L   R  H GV+S    LFVLGG +          ++    +    
Sbjct: 298 LYDPNRQLWIELAPLSMPRINH-GVLSAEGFLFVLGGQD----------ENKQTLSSGEK 357

Query: 152 FDPVTRNWSMRAPMLEARAQFACCVLDGKIIVAGGITGKRESTSKVEMYDSVKDLWVPLP 211
           +DP    W+   PM EAR  F    +DG + + GG  G RE  S +E YD     W   P
Sbjct: 358 YDPDANTWTALPPMHEARHNFGIVEIDGMLYILGGEDGDRELIS-MECYDIYSKTWTKQP 417

Query: 212 DLPEANDSGTALMVGRKMHVFSNGPIVHTFDSLEC------RWSVDTVKIKDYRRFYLDC 271
           DL      G    + +K++    G     F+S+EC      +W+     +K+ RRF    
Sbjct: 418 DLTMVRKIGCYAAMKKKIYAMGGGSYGKLFESVECYDPRTQQWTA-ICPLKE-RRFGAVA 477

Query: 272 VAVAQDSLYIMTSEYIFKQDGQDYKVFVLASQFHGRSRMAMIY----------------- 331
             VA + LY+       ++D Q  ++    S+F+       IY                 
Sbjct: 478 CGVAME-LYVF-GGVRSREDIQGSEMVTCKSEFYHDEFKRWIYLNDQNLCIPASSSFVYG 537

Query: 332 ---FGGKLYVIGGLLHHPSYAEHLSDVHVLALDSTRPTWFNAAPMSRGCGEVLGCAVLKL 366
               G  +YVIG L    +Y       +V     +  TW +  P+        GCA L++
Sbjct: 538 AVPIGASIYVIGDLDTGTNYD------YVREFKRSTGTWHHTKPLLPSDLRRTGCAALRI 575

BLAST of Spg014523 vs. ExPASy Swiss-Prot
Match: Q9LM55 (F-box/kelch-repeat protein At1g22040 OS=Arabidopsis thaliana OX=3702 GN=At1g22040 PE=2 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 8.0e-16
Identity = 63/228 (27.63%), Postives = 99/228 (43.42%), Query Frame = 0

Query: 25  LIEGLPNEVALRCLAFVPFFLLPKLELVSRSWKAAVRSVELFKARQEVGSLVDLLCVCY- 84
           LI  LP+E++++ LA +P      + LVSR W++AV + E++  R+E+G   + L V   
Sbjct: 43  LIPSLPDELSIQILARLPRICYSSVRLVSRRWRSAVSTSEVYSLRKELGRTEEWLYVLTK 102

Query: 85  -YDKDNAWQLYDPVQDLWITLPRLPT------TRKQHFGVVSTSQKLFVLG--------- 144
            ++    W   DPV   W  LP +P       +RK   G+ +     F +G         
Sbjct: 103 GHEDKLLWYALDPVSTKWQRLPPMPVVVYEEESRKSLSGLWNMITPSFNVGAIVRSFLGR 162

Query: 145 ------------------GYNLILDPLTRVLDDDDICNEVWSFDPVTRNWSMRAPMLEAR 204
                             G   ++  L+R        + VW FDP+  +WS  + ML +R
Sbjct: 163 RDSSEQMPFCGCAIGAVDGGLYVIGGLSR----SKTVSCVWRFDPILNSWSEVSSMLASR 222

Query: 205 AQFACCVLDGKIIVAGGITGKRESTS---KVEMYDSVKDLWVPLPDLP 215
           A     VL+ K+ V GG+   R   S     E+YD   D W  +P +P
Sbjct: 223 AYSKTGVLNKKLYVVGGVDRGRGGLSPLQSAEVYDPSTDAWSEVPSMP 266

BLAST of Spg014523 vs. ExPASy TrEMBL
Match: A0A6J1D7Q9 (F-box/kelch-repeat protein SKIP30-like OS=Momordica charantia OX=3673 GN=LOC111018411 PE=4 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 9.5e-105
Identity = 208/352 (59.09%), Postives = 250/352 (71.02%), Query Frame = 0

Query: 22  MSSLIEGLPNEVALRCLAFVPFFLLPKLELVSRSWKAAVRSVELFKARQEVGSLVDLLCV 81
           MS LIEGLP+ +ALRCLA VP++L PKLELVSRSWKAA RS ELF+ARQEVGS  DLLCV
Sbjct: 1   MSGLIEGLPDAIALRCLARVPYYLHPKLELVSRSWKAAFRSAELFRARQEVGSSEDLLCV 60

Query: 82  CYYDKDNAWQLYDPVQDLWITLPRLPTTRK--QHFGVVSTSQKLFVLGGYNLILDPLTRV 141
           C YD +N WQLYDP++DLWITLP LP+ R+   HFG  STSQ LFVLGG +  +DPLT  
Sbjct: 61  CSYDPNNTWQLYDPLRDLWITLPELPSKRRHLSHFGAASTSQYLFVLGGRSDAIDPLTGD 120

Query: 142 LDDDDICNEVWSFDPVTRNWSMRAPMLEARAQFACCVLDGKIIVAGGITGKRESTSKVEM 201
            DD+   NEVWSFDPVTRNW MRAPML  RA FACCVL+GKIIVAGG T   +STSK E+
Sbjct: 121 HDDNLSTNEVWSFDPVTRNWLMRAPMLVPRAMFACCVLEGKIIVAGGFTSTSKSTSKTEI 180

Query: 202 YDSVKDLWVPLPDLPEANDSG-TALMVGRKMHVFSNG-PIVHTFDSLECRWSVDTVKIKD 261
           YDS KDLWVPLPDL + +DS    +++G K+H+   G   V  FD+LE +W     +++D
Sbjct: 181 YDSEKDLWVPLPDLLQTHDSTCIGVVIGGKVHIMYKGQSTVQIFDTLELKW-----RVED 240

Query: 262 YRRFYLDCVAVAQDSLYIMTSEYIFKQDGQDYKVFVLA-SQFHGRSRMAMIYFGGKLYVI 321
           Y  +    +A  QDSLY+M    IFKQDG+D +V + A SQF  R  MAMI F G+LYVI
Sbjct: 241 Y-GWLPGPMAAVQDSLYVMCLGLIFKQDGRDRQVVIPAVSQFLHRIGMAMIGFRGELYVI 300

Query: 322 GGLLHH---PSYAEHLSDVHVLALDSTRPTWFNAAPMSRGCGEVLGCAVLKL 366
           GG++      +    LSDVHVL L   +PTW+  APMSR  G VLGC  L++
Sbjct: 301 GGVVRPVRLDADLVKLSDVHVLNLRGEKPTWYCVAPMSRCHGTVLGCTELRI 346

BLAST of Spg014523 vs. ExPASy TrEMBL
Match: A0A6J1GPT2 (F-box/kelch-repeat protein SKIP30-like OS=Cucurbita moschata OX=3662 GN=LOC111456033 PE=4 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 3.1e-103
Identity = 199/351 (56.70%), Postives = 249/351 (70.94%), Query Frame = 0

Query: 22  MSSLIEGLPNEVALRCLAFVPFFLLPKLELVSRSWKAAVRSVELFKARQEVGSLVDLLCV 81
           MS LIEGLP+ +++RCLAFVP++L PKLELVSRSWKAA+RS ELF+ARQEVGS  DLLCV
Sbjct: 1   MSGLIEGLPDAISIRCLAFVPYYLHPKLELVSRSWKAAIRSTELFRARQEVGSSEDLLCV 60

Query: 82  CYYDKDNAWQLYDPVQDLWITLPRLPT--TRKQHFGVVSTSQKLFVLGGYNLILDPLTRV 141
           C YD +N WQLYDP+Q+LW+TLP LP+  T   +FGVVSTSQKLFVLGG    +DP+T  
Sbjct: 61  CSYDPNNTWQLYDPLQNLWMTLPELPSKITHLANFGVVSTSQKLFVLGGRGDAVDPVTGD 120

Query: 142 LDDDDICNEVWSFDPVTRNWSMRAPMLEARAQFACCVLDGKIIVAGGITGKRESTSKVEM 201
            +D+   NEVWSFDPVTR WSMRAPML  R  FACCV+DGKI+VAGG T   +STS+ EM
Sbjct: 121 REDNSSTNEVWSFDPVTRTWSMRAPMLVPRYTFACCVVDGKIVVAGGFTSLSKSTSQAEM 180

Query: 202 YDSVKDLWVPLPDLPEANDSG-TALMVGRKMHVFSNG-PIVHTFDSLECRWSVDTVKIKD 261
           YD+ KD+W+PLPDL   +DS    LM+G KMHV   G   V  FDSLE +W     +++D
Sbjct: 181 YDNEKDVWIPLPDLLHTHDSTCIGLMIGGKMHVMYGGVSTVQVFDSLELKW-----RVED 240

Query: 262 YRRFYLDCVAVAQDSLYIMTSEYIFKQDGQDYKVFVLASQFHGRSRMAMIYFGGKLYVIG 321
           Y  +     AV   SLY M+   +FKQ+ +D +  V ASQF  R  MA++ F G+LYVIG
Sbjct: 241 Y-GWLPGHKAVVGGSLYTMSQGLVFKQEERDAQAVVSASQFVQRIGMAIVGFRGELYVIG 300

Query: 322 GLLHHPSY---AEHLSDVHVLALDSTRPTWFNAAPMSRGCGEVLGCAVLKL 366
           G++    +      LSDVHVL + + +P+W+ AAPMSR  G VLGC V ++
Sbjct: 301 GVVRPERFDGDLVKLSDVHVLKVRNEKPSWYCAAPMSRCSGTVLGCTVWRI 345

BLAST of Spg014523 vs. ExPASy TrEMBL
Match: A0A6J1JW98 (F-box/kelch-repeat protein SKIP30-like OS=Cucurbita maxima OX=3661 GN=LOC111488452 PE=4 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 1.3e-101
Identity = 195/351 (55.56%), Postives = 244/351 (69.52%), Query Frame = 0

Query: 22  MSSLIEGLPNEVALRCLAFVPFFLLPKLELVSRSWKAAVRSVELFKARQEVGSLVDLLCV 81
           MS LIEGLP+ +++RCLAFVP++L PKLELVSRSWKAA+RS ELF+ARQEVGS  DLLCV
Sbjct: 1   MSGLIEGLPDAISIRCLAFVPYYLHPKLELVSRSWKAAIRSTELFRARQEVGSSEDLLCV 60

Query: 82  CYYDKDNAWQLYDPVQDLWITLPRLPTTRKQ--HFGVVSTSQKLFVLGGYNLILDPLTRV 141
           C YD +N W+LYDP+Q+LW+TLP LP+  K   +FGVVSTSQKLFVLGG    +DP+T  
Sbjct: 61  CSYDPNNTWKLYDPLQNLWMTLPELPSKIKHLANFGVVSTSQKLFVLGGRGDAVDPVTGD 120

Query: 142 LDDDDICNEVWSFDPVTRNWSMRAPMLEARAQFACCVLDGKIIVAGGITGKRESTSKVEM 201
            ++    NEVWSFDPVTR WSMRAPML  R+ FACCV+DGKI+VAGG T   +STS+ EM
Sbjct: 121 REETSSTNEVWSFDPVTRMWSMRAPMLVPRSMFACCVVDGKIVVAGGFTSLSKSTSQAEM 180

Query: 202 YDSVKDLWVPLPDLPEANDSG-TALMVGRKMHVFSNG-PIVHTFDSLECRWSVDTVKIKD 261
           YD  KD+W+PLPDL    DS    LM+G KMH+   G   V  FDSLE +W V      +
Sbjct: 181 YDIEKDVWIPLPDLLHTRDSTCIGLMIGGKMHIMYGGVSTVQVFDSLELKWRV------E 240

Query: 262 YRRFYLDCVAVAQDSLYIMTSEYIFKQDGQDYKVFVLASQFHGRSRMAMIYFGGKLYVIG 321
           Y  +     AV   SLY M+   +FKQ+ +  +  V ASQF  R  MAM+ F G+LYVIG
Sbjct: 241 YYGWLPGHKAVVGGSLYTMSQGLVFKQEERGAQAVVSASQFVQRIGMAMVGFRGELYVIG 300

Query: 322 GLLHHPSY---AEHLSDVHVLALDSTRPTWFNAAPMSRGCGEVLGCAVLKL 366
           G++    +      LSDVHVL + + +P+W+ AAP SR  G +LGC VL++
Sbjct: 301 GIVRPERFDGDLVKLSDVHVLKVRNEKPSWYCAAPTSRCSGTILGCTVLRI 345

BLAST of Spg014523 vs. ExPASy TrEMBL
Match: A0A1S3BR74 (F-box/kelch-repeat protein SKIP30-like OS=Cucumis melo OX=3656 GN=LOC103492651 PE=4 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 4.9e-101
Identity = 193/349 (55.30%), Postives = 242/349 (69.34%), Query Frame = 0

Query: 22  MSSLIEGLPNEVALRCLAFVPFFLLPKLELVSRSWKAAVRSVELFKARQEVGSLVDLLCV 81
           MSSLIEGLP +VALRCLAFVPF+L P LE V RSW+ A+ S E+++ R E G   DLL V
Sbjct: 1   MSSLIEGLPRDVALRCLAFVPFYLHPTLEQVCRSWRDAICSGEIYRVRHECGKAEDLLFV 60

Query: 82  CYYDKDNAWQLYDPVQDLWITLPRLPTTRKQHFGVVSTSQKLFVLGGYNLILDPLTRVLD 141
           C +D++N WQ YDP+++ W+TLP LP  RK +FGVVST QKLF+LGG  L+++P+    D
Sbjct: 61  CCHDEENKWQFYDPIENFWVTLPELPGGRKHYFGVVSTYQKLFILGG--LLINPIDPSAD 120

Query: 142 DDDICNEVWSFDPVTRNWSMRAPMLEARAQFACCVLDGKIIVAGGITGKRESTSKVEMYD 201
           +D  CNEVWSF+P+TR WS++APM EAR+ FAC VLDG IIV GG+  K E+T K EMYD
Sbjct: 121 EDFSCNEVWSFNPMTRKWSIQAPMHEARSLFACGVLDGMIIVVGGMNKKFEATPKAEMYD 180

Query: 202 SVKDLWVPLPDLPEANDSGTAL--MVGRKMHVFSNG-PIVHTFDSLECRWSVDTVKIKDY 261
            VKD+W  LPDLP   DSG  +  +VGRKMH    G PIV TFD++E +W+V+     DY
Sbjct: 181 PVKDVWTQLPDLPRICDSGICMGVVVGRKMHFIYKGLPIVQTFDTVEWKWTVE-----DY 240

Query: 262 RRF-YLDCVAVAQDSLYIMTSEYIFKQDGQDYKVFVLASQFHGRSRMAMIYFGGKLYVIG 321
             F ++  +   +D +YIM+  YIF Q G D KV + A QFH    M MI F G+LYVIG
Sbjct: 241 NWFSHIWLMTADRDRIYIMSQGYIFLQIGLDSKVVISADQFHLNDGMGMICFRGELYVIG 300

Query: 322 GLLHHPSYAEHLSDVHVLALDST-RPTWFNAAPMSRGCGEVLGCAVLKL 366
           G L+     E+LSDVHVL L S  R  W + APMSRG G VLGCA L++
Sbjct: 301 GTLYTDHDYEYLSDVHVLTLSSDFRTCWIDIAPMSRGYGSVLGCAALRV 342

BLAST of Spg014523 vs. ExPASy TrEMBL
Match: A0A5D3DRI4 (F-box/kelch-repeat protein SKIP30-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold111G001440 PE=4 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 8.3e-101
Identity = 193/349 (55.30%), Postives = 242/349 (69.34%), Query Frame = 0

Query: 22  MSSLIEGLPNEVALRCLAFVPFFLLPKLELVSRSWKAAVRSVELFKARQEVGSLVDLLCV 81
           MSSLIEGLP +VALRCLAFVPF+L P LE V RSW+ A+ S E+++ R E G   DLL V
Sbjct: 1   MSSLIEGLPRDVALRCLAFVPFYLHPTLEQVCRSWRDAICSGEIYRVRHECGKAEDLLFV 60

Query: 82  CYYDKDNAWQLYDPVQDLWITLPRLPTTRKQHFGVVSTSQKLFVLGGYNLILDPLTRVLD 141
           C +D++N WQ YDP+++ W+TLP LP  RK +FGVVST QKLF+LGG  L+++P+    D
Sbjct: 61  CCHDEENKWQFYDPIENFWVTLPELPGGRKHYFGVVSTYQKLFILGG--LLINPIDPSAD 120

Query: 142 DDDICNEVWSFDPVTRNWSMRAPMLEARAQFACCVLDGKIIVAGGITGKRESTSKVEMYD 201
           +D  CNEVWSF+P+TR WS++APM EAR+ FAC VLDG IIV GG+  K E+T K EMYD
Sbjct: 121 EDFSCNEVWSFNPMTRKWSIQAPMHEARSLFACGVLDGMIIVVGGMNKKFEATPKAEMYD 180

Query: 202 SVKDLWVPLPDLPEANDSGTAL--MVGRKMHVFSNG-PIVHTFDSLECRWSVDTVKIKDY 261
            VKD+W  LPDLP   DSG  +  +VGRKMH    G PIV TFD++E +W+V+     DY
Sbjct: 181 PVKDVWTQLPDLPRICDSGICMGVVVGRKMHFIYKGLPIVQTFDTVEWKWTVE-----DY 240

Query: 262 RRF-YLDCVAVAQDSLYIMTSEYIFKQDGQDYKVFVLASQFHGRSRMAMIYFGGKLYVIG 321
             F ++  +   +D +YIM+  YIF Q G D KV + A QFH    M MI F G+LYVIG
Sbjct: 241 NWFSHIWLMTADRDRIYIMSQGYIFLQIGLDSKVVISADQFHLNDGMGMICFRGELYVIG 300

Query: 322 GLLHHPSYAEHLSDVHVLALDST-RPTWFNAAPMSRGCGEVLGCAVLKL 366
           G L+     E+LSDVHVL L S  R  W + APMSRG G VLGCA L++
Sbjct: 301 GTLYTDHDYEYLSDVHVLTLSSDFRTCWTDIAPMSRGYGSVLGCAALRV 342

BLAST of Spg014523 vs. TAIR 10
Match: AT3G63220.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 289.3 bits (739), Expect = 4.4e-78
Identity = 155/351 (44.16%), Postives = 214/351 (60.97%), Query Frame = 0

Query: 22  MSSLIEGLPNEVALRCLAFVPFFLLPKLELVSRSWKAAVRSVELFKARQEVGSLVDLLCV 81
           MS L++G+P  VALRCLA VP  L P LELVSRSW+AA+RS ELF+ R+E+ S   LLCV
Sbjct: 1   MSGLLDGIPEAVALRCLAHVPLHLHPNLELVSRSWRAAIRSHELFRVRKELRSSEHLLCV 60

Query: 82  CYYDKDNAWQLYDPVQDLWITLPRLPTTRKQ--HFGVVSTSQKLFVLGGYNLILDPLTRV 141
           C +D +N WQ+Y P  D W+TLP LP+  +   HFG V+T+  LFVLGG +  + P+T  
Sbjct: 61  CAFDPENIWQVYSPNCDRWLTLPLLPSRIRHLAHFGAVTTAGMLFVLGGGSDAVSPVTGD 120

Query: 142 LDDDDICNEVWSFDPVTRNWSMRAPMLEARAQFACCVLDGKIIVAGGITGKRESTSKVEM 201
            D     ++VWS+D V R W+ RA ML  RA FACCVL GKI+VAGG T  R+S S  EM
Sbjct: 121 HDGTFATDQVWSYDFVQRQWTPRASMLVPRAMFACCVLQGKIVVAGGFTTCRKSISGAEM 180

Query: 202 YDSVKDLWVPLPDLPEANDSG-TALMVGRKMHVFSNG-PIVHTFDSLECRWSVDTVKIKD 261
           YD   D+W  +PDL + ++S  + L+V  K+HV   G   V   +S++  W V     KD
Sbjct: 181 YDPENDVWTSIPDLHQTHNSACSGLVVNGKVHVLHKGLSTVQVLESVKLGWDV-----KD 240

Query: 262 YRRFYLDCVAVAQDSLYIMTSEYIFKQDGQDYKVFVLASQFHGRSRMAMIYFGGKLYVIG 321
           Y  +    + V +D LY+M+   +FKQ+G  +K+   AS+F  R  MAM     ++ ++G
Sbjct: 241 Y-GWPQGPMVVVEDVLYVMSHGLVFKQEGDTWKMVASASEFKRRIGMAMTSLSDEVLIVG 300

Query: 322 GLLHHPSY---AEHLSDVHVLALDSTRPTWFNAAPMSRGCGEVLGCAVLKL 366
           G++         + LSDV  L + + RP W + APM+R  G +LGC  L +
Sbjct: 301 GVIGPDRLNWDIKPLSDVDALTVGNDRPAWRSVAPMTRCRGTILGCTQLTI 345

BLAST of Spg014523 vs. TAIR 10
Match: AT3G63220.2 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 289.3 bits (739), Expect = 4.4e-78
Identity = 155/351 (44.16%), Postives = 214/351 (60.97%), Query Frame = 0

Query: 22  MSSLIEGLPNEVALRCLAFVPFFLLPKLELVSRSWKAAVRSVELFKARQEVGSLVDLLCV 81
           MS L++G+P  VALRCLA VP  L P LELVSRSW+AA+RS ELF+ R+E+ S   LLCV
Sbjct: 8   MSGLLDGIPEAVALRCLAHVPLHLHPNLELVSRSWRAAIRSHELFRVRKELRSSEHLLCV 67

Query: 82  CYYDKDNAWQLYDPVQDLWITLPRLPTTRKQ--HFGVVSTSQKLFVLGGYNLILDPLTRV 141
           C +D +N WQ+Y P  D W+TLP LP+  +   HFG V+T+  LFVLGG +  + P+T  
Sbjct: 68  CAFDPENIWQVYSPNCDRWLTLPLLPSRIRHLAHFGAVTTAGMLFVLGGGSDAVSPVTGD 127

Query: 142 LDDDDICNEVWSFDPVTRNWSMRAPMLEARAQFACCVLDGKIIVAGGITGKRESTSKVEM 201
            D     ++VWS+D V R W+ RA ML  RA FACCVL GKI+VAGG T  R+S S  EM
Sbjct: 128 HDGTFATDQVWSYDFVQRQWTPRASMLVPRAMFACCVLQGKIVVAGGFTTCRKSISGAEM 187

Query: 202 YDSVKDLWVPLPDLPEANDSG-TALMVGRKMHVFSNG-PIVHTFDSLECRWSVDTVKIKD 261
           YD   D+W  +PDL + ++S  + L+V  K+HV   G   V   +S++  W V     KD
Sbjct: 188 YDPENDVWTSIPDLHQTHNSACSGLVVNGKVHVLHKGLSTVQVLESVKLGWDV-----KD 247

Query: 262 YRRFYLDCVAVAQDSLYIMTSEYIFKQDGQDYKVFVLASQFHGRSRMAMIYFGGKLYVIG 321
           Y  +    + V +D LY+M+   +FKQ+G  +K+   AS+F  R  MAM     ++ ++G
Sbjct: 248 Y-GWPQGPMVVVEDVLYVMSHGLVFKQEGDTWKMVASASEFKRRIGMAMTSLSDEVLIVG 307

Query: 322 GLLHHPSY---AEHLSDVHVLALDSTRPTWFNAAPMSRGCGEVLGCAVLKL 366
           G++         + LSDV  L + + RP W + APM+R  G +LGC  L +
Sbjct: 308 GVIGPDRLNWDIKPLSDVDALTVGNDRPAWRSVAPMTRCRGTILGCTQLTI 352

BLAST of Spg014523 vs. TAIR 10
Match: AT1G16250.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 106.3 bits (264), Expect = 5.3e-23
Identity = 74/221 (33.48%), Postives = 96/221 (43.44%), Query Frame = 0

Query: 21  IMSSLIEGLPNEVALRCLAFVPFFLLPKLELVSRSWKAAVRSVELFKARQEVGSLVDLLC 80
           I  S+I GLP+++ALRC+A +       LE VSR W+  VR  +    +   G     L 
Sbjct: 4   IEQSIIPGLPDDLALRCIAKLSHGYHGVLECVSRGWRDLVRGADYSCYKARNGWSGSWLF 63

Query: 81  VCYYDKDNAWQLYDPVQDLWITLPRLPTTR----KQHFGVVSTSQKLFVLGG-YNLILDP 140
           V      N W  YDP  D W  LPR    +       F  V  S  L V+GG Y   +  
Sbjct: 64  VLTERSKNQWVAYDPEADRWHPLPRTRAVQDGWHHSGFACVCVSNCLLVIGGCYAPSVSS 123

Query: 141 LTRVLDDDDICNEVWSFDPVTRNWSMRAPMLEARAQFACCVLDGKIIVAGG--ITGKRES 200
                    +  +V  FDP  + W M A M   R  FAC  + GK+ VAGG  +T  R  
Sbjct: 124 FPH--QKPVVTKDVMRFDPFKKQWKMVASMRTPRTHFACTSVSGKVYVAGGRNLTHSRGI 183

Query: 201 TSKVEMYDSVKDLWVPLPDLPEANDSGTALMVGRKMHVFSN 235
            S  E+YD V D W  LP +P      + L      HV S+
Sbjct: 184 PS-AEVYDPVADRWEELPAMPRPQMDCSGLSYRGCFHVLSD 221

BLAST of Spg014523 vs. TAIR 10
Match: AT1G67480.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 104.8 bits (260), Expect = 1.5e-22
Identity = 70/235 (29.79%), Postives = 103/235 (43.83%), Query Frame = 0

Query: 25  LIEGLPNEVALRCLAFVPFFLLPKLELVSRSWKAAVRSVELFKARQEVGSLVDLLCVCYY 84
           LI GLP++VA +CLA VP    P +  V + W+  V+S E    R+  G L + L V   
Sbjct: 39  LIPGLPDDVAKQCLALVPRARFPSMGSVCKKWRFVVQSKEFITVRRLAGMLEEWLYVLTM 98

Query: 85  D---KDNAWQLYDPVQDLWITLPRLPTTRKQHFGVVSTSQKLFVLGGYNLILDPLTRVLD 144
           +   KDN W++ D +     +LP +P   K  F VV    KL V+ G  +I   L    D
Sbjct: 99  NAGGKDNRWEVMDCLGQKLSSLPPMPGPAKTGFKVVVVDGKLLVIAGCCMINGSLVASAD 158

Query: 145 DDDICNEVWSFDPVTRNWSMRAPMLEARAQFACCVLDGKIIVAGGITGKRESTSKVEMYD 204
                  V+ +D    +WS  A +  AR  FAC  ++G + V GG     ES S  E+YD
Sbjct: 159 -------VYQYDTCLNSWSRLADLEVARYDFACAEVNGHVYVVGGHGVDGESLSSAEVYD 218

Query: 205 SVKDLWVPLPDL--------PEANDSGTALMVGRKMHVFSNGPIVHTFDSLECRW 249
                W  +  L          A +    +M GR      N  ++  +++    W
Sbjct: 219 PETCTWTFIESLRRPRWGCFASAFNGKLYVMGGRSNFTIGNSKLLDVYNTQCGSW 266

BLAST of Spg014523 vs. TAIR 10
Match: AT1G67480.2 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 104.8 bits (260), Expect = 1.5e-22
Identity = 70/235 (29.79%), Postives = 103/235 (43.83%), Query Frame = 0

Query: 25  LIEGLPNEVALRCLAFVPFFLLPKLELVSRSWKAAVRSVELFKARQEVGSLVDLLCVCYY 84
           LI GLP++VA +CLA VP    P +  V + W+  V+S E    R+  G L + L V   
Sbjct: 39  LIPGLPDDVAKQCLALVPRARFPSMGSVCKKWRFVVQSKEFITVRRLAGMLEEWLYVLTM 98

Query: 85  D---KDNAWQLYDPVQDLWITLPRLPTTRKQHFGVVSTSQKLFVLGGYNLILDPLTRVLD 144
           +   KDN W++ D +     +LP +P   K  F VV    KL V+ G  +I   L    D
Sbjct: 99  NAGGKDNRWEVMDCLGQKLSSLPPMPGPAKTGFKVVVVDGKLLVIAGCCMINGSLVASAD 158

Query: 145 DDDICNEVWSFDPVTRNWSMRAPMLEARAQFACCVLDGKIIVAGGITGKRESTSKVEMYD 204
                  V+ +D    +WS  A +  AR  FAC  ++G + V GG     ES S  E+YD
Sbjct: 159 -------VYQYDTCLNSWSRLADLEVARYDFACAEVNGHVYVVGGHGVDGESLSSAEVYD 218

Query: 205 SVKDLWVPLPDL--------PEANDSGTALMVGRKMHVFSNGPIVHTFDSLECRW 249
                W  +  L          A +    +M GR      N  ++  +++    W
Sbjct: 219 PETCTWTFIESLRRPRWGCFASAFNGKLYVMGGRSNFTIGNSKLLDVYNTQCGSW 266

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7014455.11.5e-10456.74F-box/kelch-repeat protein SKIP30 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022150165.12.0e-10459.09F-box/kelch-repeat protein SKIP30-like [Momordica charantia][more]
XP_011659773.16.3e-10355.34F-box/kelch-repeat protein SKIP30 [Cucumis sativus][more]
XP_022953500.16.3e-10356.70F-box/kelch-repeat protein SKIP30-like [Cucurbita moschata] >XP_022953501.1 F-bo... [more]
XP_038896848.18.3e-10356.16F-box/kelch-repeat protein SKIP30-like [Benincasa hispida] >XP_038896849.1 F-box... [more]
Match NameE-valueIdentityDescription
Q9M1W76.2e-7744.16F-box/kelch-repeat protein SKIP30 OS=Arabidopsis thaliana OX=3702 GN=SKIP30 PE=1... [more]
Q0WW407.4e-2233.48F-box/kelch-repeat protein At1g16250 OS=Arabidopsis thaliana OX=3702 GN=At1g1625... [more]
Q9CAG82.2e-2129.79F-box/kelch-repeat protein At1g67480 OS=Arabidopsis thaliana OX=3702 GN=At1g6748... [more]
Q8CA722.9e-1827.00Gigaxonin OS=Mus musculus OX=10090 GN=Gan PE=1 SV=2[more]
Q9LM558.0e-1627.63F-box/kelch-repeat protein At1g22040 OS=Arabidopsis thaliana OX=3702 GN=At1g2204... [more]
Match NameE-valueIdentityDescription
A0A6J1D7Q99.5e-10559.09F-box/kelch-repeat protein SKIP30-like OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A6J1GPT23.1e-10356.70F-box/kelch-repeat protein SKIP30-like OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
A0A6J1JW981.3e-10155.56F-box/kelch-repeat protein SKIP30-like OS=Cucurbita maxima OX=3661 GN=LOC1114884... [more]
A0A1S3BR744.9e-10155.30F-box/kelch-repeat protein SKIP30-like OS=Cucumis melo OX=3656 GN=LOC103492651 P... [more]
A0A5D3DRI48.3e-10155.30F-box/kelch-repeat protein SKIP30-like OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
Match NameE-valueIdentityDescription
AT3G63220.14.4e-7844.16Galactose oxidase/kelch repeat superfamily protein [more]
AT3G63220.24.4e-7844.16Galactose oxidase/kelch repeat superfamily protein [more]
AT1G16250.15.3e-2333.48Galactose oxidase/kelch repeat superfamily protein [more]
AT1G67480.11.5e-2229.79Galactose oxidase/kelch repeat superfamily protein [more]
AT1G67480.21.5e-2229.79Galactose oxidase/kelch repeat superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006652Kelch repeat type 1SMARTSM00612kelc_smartcoord: 180..227
e-value: 1.6E-4
score: 31.0
coord: 122..179
e-value: 8.4E-7
score: 38.6
IPR006652Kelch repeat type 1PFAMPF01344Kelch_1coord: 113..165
e-value: 4.7E-5
score: 22.9
coord: 168..214
e-value: 7.4E-13
score: 47.9
coord: 301..351
e-value: 3.6E-4
score: 20.1
IPR001810F-box domainSMARTSM00256fbox_2coord: 29..69
e-value: 6.1E-5
score: 32.4
IPR001810F-box domainPFAMPF00646F-boxcoord: 26..66
e-value: 3.8E-4
score: 20.2
IPR001810F-box domainPROSITEPS50181FBOXcoord: 23..69
score: 8.888098
IPR015915Kelch-type beta propellerGENE3D2.120.10.80coord: 78..252
e-value: 6.2E-34
score: 119.2
IPR015915Kelch-type beta propellerGENE3D2.120.10.80coord: 253..363
e-value: 1.2E-5
score: 26.3
IPR015915Kelch-type beta propellerSUPERFAMILY117281Kelch motifcoord: 80..351
NoneNo IPR availablePANTHERPTHR24414F-BOX/KELCH-REPEAT PROTEIN SKIP4coord: 23..365
NoneNo IPR availablePANTHERPTHR24414:SF40F-BOX/KELCH-REPEAT PROTEIN SKIP30coord: 23..365
IPR036047F-box-like domain superfamilySUPERFAMILY81383F-box domaincoord: 25..66

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg014523.1Spg014523.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding