Spg014522 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg014522
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionRING-type E3 ubiquitin transferase
Locationscaffold3: 49251620 .. 49256918 (-)
RNA-Seq ExpressionSpg014522
SyntenySpg014522
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCCTCATCGGAGCTAGCCCTCGTTTCTCTGGTCTCCCGGTTTGCTTTATCGTTTGATGGAGCCGTTTTGGGCGCAGCCCTAGCTTACGCTACCGTTCGTACCGTCCTCAAACTCACTGCTTCCTGTTCCGCTCTCAAAGAGCTCCGTAAAGCTCCTTATGTTAGAGTTTCTGATCTTCGCCATATCATTTCTGAAGGAGATTCGGAACAATCCGGGTCTGATCAGAAGCTTGTTGTAGTTCGTGGCACCGTGGAGGCCAGATCGTCCGTGGAGGGTAATTGGAAGAGCTTACAACCTAATGTATTGATTTCTCAAGAGTCCGGAGATAGAGCGGTGATTATTCAGCGAACTCAAATGGTAACTCCTCTGGTATTTCTGTTAGTATCTGCGTCATTGTGATGATACATATATGTATTCTCTTTTTGGGGCTGCCGTTCTTCTGTAGGGGGATGGATGAAAACTGAGCTATGCAGTTTAGATTGCGTGATTAAAATTAATATTATTCCGTTTGTTAGAATGTTATGATTGCATAGTGTCGTGACAGTATTCGAGTAATTTGATAGCACCACTAAGATTGCTTATAAAGTTTTAACCAATTAAATTTTTTTCACATTACAAGTTCTCTAGTTTAGGTCCTCGGTTCAAGAGGCAGTTAAGATATGATTCTAGTCTGGATGAACAATGAAAGAGAAGGATCACCGTTTGAAATGACTGTTGAATTTATTTGCATAGTTCGTCTGGCTTGCCTGTGTTATATCTGTCACTCATTAATTTGTATAAGTATGATGTGCGGATTAAATATTGGGAAATCTTGAACTCCTTAATCAACATAATTAACTTATGAATTTAATCTTGGGGAAATGCATATCTCTTTTGCCAGTGTTACCTATCTTAACCTCATTGGTTCTGCAATTGTCTGTTTTTGTTCCCCTTGTTATTCAGTGTTATGGATGTGTGTATATAATATCCAGTATTATAAATTGAAGCCTTTTTGGTTGTAAATTCTGGCATTGTGTAGTGCATATTCAACGAATGGAAAGGCTTTTTTGGATGGACTTCTGATATACGTGCAATTATTGGAAGAACCTTTAGAAAGCAAGAGTCAACATCATTTAGAACGGTAAAGTCAAGGCTTCTTTCTATTATTTCTATGATTGATTCACTCTTTTCGTTACCCTTACTGACAAAAGATCTTTGTATTATATATAGCCATTTACCTTTCCACTTGAATGATGGACCTTTGTTACTCCTTCTATGAATAAAGAACCAATTGAAGTTGAATGATTTTTTATGTGGGGCATCTCTTGAATATGGGGCCATTCTGTTAGGTCCTTTTCCTGTGATTCTCTATTGGGTCTTTTTTTTTCTCTCGCTCATGGTGCGTGCAATTAGGTTTGTAAGTGCCACCTCTTTAGAAACGAGTTTTAACAGAGTAGGCTTGAAGCCCTCGCTATGGCTGAGATGATGGGGTGTAGGATTGAGAAAATTCCGTTTAACTACGTGGGGGTCTCTGTTGGCGGTAGGCCTAAGAAGGAAGCGTTCTGGAACCGGTAATAGACAACATTCGCAGCAAGTTAGCTCTTTGGAAAGGTTTCCCCATCTCCAAAGGTGGTAGAACTACCCTCATAAAGGCTGTTTTATCTAGCATCCCAGCCTGTTTTCTTTCTATTTTCAGGATACCAGTGGGGGTGAGCAAATGCATTGAGAAGTATTTTAGAAGATTCCTTTGGAAAGGGGCTGATGATATTAAAGGTTCCCATTTGGTGAATTGGGAGGCTGTGACAACTCTTAAATCGTTAGGAGGGCTTGGTATTGATAACATAAGAGCTAAGAACATGGCTCTTCTCTCAAAGTGGCTTTGGAGATTTGAAAAGGAGCCCGAGGCCTTATGGAGGAAGATCATTCTCAGTAAGTATGGGGATGGCTTTACTCGAGACCCCTTGAGCTATAAAAAGTGGAAATCGGTTTCCAGTTCGTGGGAGGATATAGCTAGATTAGCTGGTTTCTTAAAAAATAATAGTAAGTTAAGTATAAGGTGGGTAAAGGGGACAAAGCTTTGTTTTGGGAGGATGCCTGAGTGTCTGAAAGGCCCTTAAAGGATATGTTCCCTCGTATGTTTGTCATTTCGATGGCTAAAAAGGTGAAAGTTAACGAGCTTTGGAATGACTACTCCTGGGATCTTAGACTTAGAAGAGGAATGCTCGATAGGGAGGCTAACAAATGGTTTGGGCTTTGCTCTACTTTATCTCAGCACAGTTTCAACGGTAATGATGATGTCATTTGGTGGAAGCCTGAAAACTCTGGCATTTTCTCTGTTAAATCTGCTCTCTTAGCTTTGCAGCTGAATAGAAGATTGTTGGTGGAGGAGTTGAGCTCTCAAATATGGGAAGGTACCATTCCTAATAGGGTGAAGTTCTTCTTTTGGACTTTAGCTCTTAATGGAATCAACACTATGGACATGGTGCAGAACATGTGTGTTATGTGCTGTAGAAATGAGGAATCGGCTTCTCACCTATTGCTTCATTGTGATTTTGCGGTTCAAGTGTGGAATCGGTTTGCTGAAAGCTTTGGGCAGAAAGGTTGCAAGCCCAGAAATGTGGTGGACTGGTTACCTGAGATTTTGGTTGGAGGAGGATTAAAAGACAAAGCAAGAATCCTGTGGAAAAATTTCTCTAGAGCCATCATTTGGCACCTATGGAAGGAAAGAAATGCAAGGGTCTTCGCAGATAAGTTCTAGATATTTTTTGTGATATTGTACAGCTTTCGGCCTCTAATTGGAGTGCCCAAGATAGGTTATTTTGTAATTACTCAGCCGCCATGATTAACCTTCAATGGAAGGCTTTTTTGTAGTTTCTTTGGAGGTAGGGATGCCTTATCCCACCGTCCTTAGGCTGCTTTGGTTGTTGGCTTTTGTGAATGAAGATGTCTATTTCTTCTCTTAAAAAAAAAAAAAAAAAAAACAGAGTAGGCTTGAAGACATACCCAATAGTGTCAAGAAATAAGGAATTTATGGTAGCTAACTTGTTGTCTTTAAAAATTAAATCTACTTAATATGAGATTGATGTGTAGCCTTTGTGCTTCCTCCAGCCCCGCTATGGTTGTGATTTGTGAAGGTAAATTATATATTTTAGATATCTGGTTGAGCAACAGGGAACTATATTCCACTTATGATTGTACCAGAGTTCAGTGCAGTTTGCATATAAACTCTTATGTAATGCTGATATTTGAGAAATTTTTAACAGGTTCCTTTTGTCCTCGTTGAGGGTCCTTCACCATATTCTGATTTTATTGTTGTTAACATGGATGGCTCAAGACATCCCTTGCCTCTTACAACTGTGTATCATCAACTGGAGCCCGTTTGTGCTACTCCTTATACCTTCCTTCAAGCACTTTTTGGTCATGAGTACCCTGTGAGTTAGGCAATCTATATTGAAGTTGAATAACTTTTCTTTTATGGATTTTAGCTAAAATCTCTTTATCTTATTCTACTTTTGAAGGTTGGGGTGCTTGATGAGGAGAAAATACTTCCATTAGGAAAAAATATCAGTGCTGTTGGCATATGCAGTTTTAGAAATGGAGTTCCCGTGATAAAGTCATGCAAGGATTTTCCCCATTTCCTGTAAGATTATACATTTTGAAGTTGAAATAAAGTTGAGAAGGTCAAAATTATGTTATGTAATTTATGAAAGGTAGATCATATATGCATATCGTTTATTCTTTCCCTTGACTCATCCACATAAAATTGATAATAAATGTGTTGTTGTCAAACAGTGAGCCTCATTGATTTGGGTTTGGGGCTCTCGATAGGATTTTAACTAGGTCCTAGGATCAAGCATCTGAATAGAAAGCTTAAAAAGAAAACTTTGTCTGTTGGAGCTATATGGATTTGTATGGATTTGTTGTATAGGTCCCAATACTCTCTCTCTACACACACACACATGTGTGTGTTCCCATTTCCAGTTGAAGTGTGGGTTTTGGCTTATACCTAAGTTAATGATGCTACCAAATGGCTAGTCTCTCCCACACCCACCCAAAGAAAAAAAAAGAATAAATTGATTTTGCCTTTTGTTTGTAATAAACTGACTTCGAGATAATCTACATTGGAATAACGGATGTTATGGTGATATACTTTTCTTTGCAGATGTGAAATGACCAAGGATCAGATGATTTTAGATCTAGTCTTCAAAACAAAGATTCTGTTCTGGAGTAGTATTGTCCTTGGTTCGTTGTCAGTTGGCATTCTTGGCTATGCTGTTGTGAGGTATGCTTCCATTCTTTCTTAGTTTCTTTCTTCTTTTATTTTATTTTCTTGTCAAACTGTCGTGAGGTATCTTAATGTTGATATTCTTTTAGGCGAAGCAATGCACTCGTAATATATACCTTCCATGAAATGTTTATTTTGGATTCAATGCTGAAATGATCTTCAATCTTTTCTTTTTTGCCGTTGAATTTCTTTTACTTTTATGAACTCACCAATGTTCTTAATTGGACCTCAAATGCTGGTATGAATAATAACTTAGCTCAACTGTGTAGAGCGTGAGTGATGAATGACGAGACTGTTTCCAAGTATACTCACTTGTTTGGTTGCTTTCCTTCATCAAAAAATGATTACATTGTAAAACTTTGATGAATGTTTGAACTTAGTAGTGTCCATTCTCTTCAGAAATTGGAATAAATGGAAACGGTGGACGCAACAGGGACGGTTACAGGATTCAGGGAATGATTCCACCATGTCCCAACTGGATTCAAGCAATAATATTCCCATTTATGGTGATTCTGGAACTGCCTTAGACGACGAGGTAGCAGCGGTTTCAGATGACGAGGTATTGAACATTCCAGATGGACAGTTGTGCGTGATCTGTCTCATGAGGAGAAAGCGATCTGCTTTCATTCCATGTGGTCATTTAGTTTGCTGCGAACGCTGTGCCATATCGGTCGAACGTGATTCAGCTCCCAAATGTCCTGTCTGTCGCCAGCAGATCCATCGTTCTGTGCGGATATATGACTCATGAGGAGAAACAAATTATCCTTTTCAGGTTCTAACACATCTATATAGGTGACCCTTTTTGCTGATACTATTTGAGATTTTATTGTATAATGTATGCAAGTGATTCCCGAGGCAGCAGTTCTGGAGTTTGTTTCTGAACATCTATTGTAATTAGTTGTCCTTATATTCGTTATTTTGATGACAGAGCTGGTAACTTCCATGATAGCTCTTGTAAATACCAGATAATATACTCTCTCACACTGTAACTTGCTGATATGTGATGAAAATAAACTTCTATATTGAAGGCAC

mRNA sequence

ATGTCCTCATCGGAGCTAGCCCTCGTTTCTCTGGTCTCCCGGTTTGCTTTATCGTTTGATGGAGCCGTTTTGGGCGCAGCCCTAGCTTACGCTACCGTTCGTACCGTCCTCAAACTCACTGCTTCCTGTTCCGCTCTCAAAGAGCTCCGTAAAGCTCCTTATGTTAGAGTTTCTGATCTTCGCCATATCATTTCTGAAGGAGATTCGGAACAATCCGGGTCTGATCAGAAGCTTGTTGTAGTTCGTGGCACCGTGGAGGCCAGATCGTCCGTGGAGGGTAATTGGAAGAGCTTACAACCTAATGTATTGATTTCTCAAGAGTCCGGAGATAGAGCGGTGATTATTCAGCGAACTCAAATGGTAACTCCTCTGGTATTTCTTGCATATTCAACGAATGGAAAGGCTTTTTTGGATGGACTTCTGATATACGTGCAATTATTGGAAGAACCTTTAGAAAGCAAGAGTCAACATCATTTAGAACGGCCCTTAAAGGATATGTTCCCTCGTATGTTTGTCATTTCGATGGCTAAAAAGGTGAAAGTTAACGAGCTTTGGAATGACTACTCCTGGGATCTTAGACTTAGAAGAGGAATGCTCGATAGGGAGGCTAACAAATGGTTTGGGCTTTGCTCTACTTTATCTCAGCACAGTTTCAACGGTAATGATGATGTCATTTGGTGGAAGCCTGAAAACTCTGGCATTTTCTCTGTTAAATCTGCTCTCTTAGCTTTGCAGCTGAATAGAAGATTGTTGGTGGAGGAGTTGAGCTCTCAAATATGGGAAGGTACCATTCCTAATAGGGTGAAGTTCTTCTTTTGGACTTTAGCTCTTAATGGAATCAACACTATGGACATGGTGCAGAACATGTGTGTTATGTGCTGTAGAAATGAGGAATCGGCTTCTCACCTATTGCTTCATTGTGATTTTGCGGTTCAAGTGTGGAATCGGTTTGCTGAAAGCTTTGGGCAGAAAGGTTGCAAGCCCAGAAATGTGGTGGACTGGTTACCTGAGATTTTGGTTGGAGGAGGATTAAAAGACAAAGCAAGAATCCTGTGGAAAAATTTCTCTAGAGCCATCATTTGGCACCTATGGAAGGAAAGAAATGCAAGGGTTCCTTTTGTCCTCGTTGAGGGTCCTTCACCATATTCTGATTTTATTGTTGTTAACATGGATGGCTCAAGACATCCCTTGCCTCTTACAACTGTGTATCATCAACTGGAGCCCGTTTGTGCTACTCCTTATACCTTCCTTCAAGCACTTTTTGGTCATGAGTACCCTGTTGGGGTGCTTGATGAGGAGAAAATACTTCCATTAGGAAAAAATATCAGTGCTGTTGGCATATGCAGTTTTAGAAATGGAGTTCCCGTGATAAAGTCATGCAAGGATTTTCCCCATTTCCTATGTGAAATGACCAAGGATCAGATGATTTTAGATCTAGTCTTCAAAACAAAGATTCTGTTCTGGAGTAGTATTGTCCTTGGTTCGTTGTCAGTTGGCATTCTTGGCTATGCTGTTGTGAGAAATTGGAATAAATGGAAACGGTGGACGCAACAGGGACGGTTACAGGATTCAGGGAATGATTCCACCATGTCCCAACTGGATTCAAGCAATAATATTCCCATTTATGGTGATTCTGGAACTGCCTTAGACGACGAGGTAGCAGCGGTTTCAGATGACGAGGTATTGAACATTCCAGATGGACAGTTGTGCGTGATCTGTCTCATGAGGAGAAAGCGATCTGCTTTCATTCCATGTGGTCATTTAGTTTGCTGCGAACGCTGTGCCATATCGGTCGAACGTGATTCAGCTCCCAAATGTCCTGTCTGTCGCCAGCAGATCCATCGTTCTGTGCGGATATATGACTCATGA

Coding sequence (CDS)

ATGTCCTCATCGGAGCTAGCCCTCGTTTCTCTGGTCTCCCGGTTTGCTTTATCGTTTGATGGAGCCGTTTTGGGCGCAGCCCTAGCTTACGCTACCGTTCGTACCGTCCTCAAACTCACTGCTTCCTGTTCCGCTCTCAAAGAGCTCCGTAAAGCTCCTTATGTTAGAGTTTCTGATCTTCGCCATATCATTTCTGAAGGAGATTCGGAACAATCCGGGTCTGATCAGAAGCTTGTTGTAGTTCGTGGCACCGTGGAGGCCAGATCGTCCGTGGAGGGTAATTGGAAGAGCTTACAACCTAATGTATTGATTTCTCAAGAGTCCGGAGATAGAGCGGTGATTATTCAGCGAACTCAAATGGTAACTCCTCTGGTATTTCTTGCATATTCAACGAATGGAAAGGCTTTTTTGGATGGACTTCTGATATACGTGCAATTATTGGAAGAACCTTTAGAAAGCAAGAGTCAACATCATTTAGAACGGCCCTTAAAGGATATGTTCCCTCGTATGTTTGTCATTTCGATGGCTAAAAAGGTGAAAGTTAACGAGCTTTGGAATGACTACTCCTGGGATCTTAGACTTAGAAGAGGAATGCTCGATAGGGAGGCTAACAAATGGTTTGGGCTTTGCTCTACTTTATCTCAGCACAGTTTCAACGGTAATGATGATGTCATTTGGTGGAAGCCTGAAAACTCTGGCATTTTCTCTGTTAAATCTGCTCTCTTAGCTTTGCAGCTGAATAGAAGATTGTTGGTGGAGGAGTTGAGCTCTCAAATATGGGAAGGTACCATTCCTAATAGGGTGAAGTTCTTCTTTTGGACTTTAGCTCTTAATGGAATCAACACTATGGACATGGTGCAGAACATGTGTGTTATGTGCTGTAGAAATGAGGAATCGGCTTCTCACCTATTGCTTCATTGTGATTTTGCGGTTCAAGTGTGGAATCGGTTTGCTGAAAGCTTTGGGCAGAAAGGTTGCAAGCCCAGAAATGTGGTGGACTGGTTACCTGAGATTTTGGTTGGAGGAGGATTAAAAGACAAAGCAAGAATCCTGTGGAAAAATTTCTCTAGAGCCATCATTTGGCACCTATGGAAGGAAAGAAATGCAAGGGTTCCTTTTGTCCTCGTTGAGGGTCCTTCACCATATTCTGATTTTATTGTTGTTAACATGGATGGCTCAAGACATCCCTTGCCTCTTACAACTGTGTATCATCAACTGGAGCCCGTTTGTGCTACTCCTTATACCTTCCTTCAAGCACTTTTTGGTCATGAGTACCCTGTTGGGGTGCTTGATGAGGAGAAAATACTTCCATTAGGAAAAAATATCAGTGCTGTTGGCATATGCAGTTTTAGAAATGGAGTTCCCGTGATAAAGTCATGCAAGGATTTTCCCCATTTCCTATGTGAAATGACCAAGGATCAGATGATTTTAGATCTAGTCTTCAAAACAAAGATTCTGTTCTGGAGTAGTATTGTCCTTGGTTCGTTGTCAGTTGGCATTCTTGGCTATGCTGTTGTGAGAAATTGGAATAAATGGAAACGGTGGACGCAACAGGGACGGTTACAGGATTCAGGGAATGATTCCACCATGTCCCAACTGGATTCAAGCAATAATATTCCCATTTATGGTGATTCTGGAACTGCCTTAGACGACGAGGTAGCAGCGGTTTCAGATGACGAGGTATTGAACATTCCAGATGGACAGTTGTGCGTGATCTGTCTCATGAGGAGAAAGCGATCTGCTTTCATTCCATGTGGTCATTTAGTTTGCTGCGAACGCTGTGCCATATCGGTCGAACGTGATTCAGCTCCCAAATGTCCTGTCTGTCGCCAGCAGATCCATCGTTCTGTGCGGATATATGACTCATGA

Protein sequence

MSSSELALVSLVSRFALSFDGAVLGAALAYATVRTVLKLTASCSALKELRKAPYVRVSDLRHIISEGDSEQSGSDQKLVVVRGTVEARSSVEGNWKSLQPNVLISQESGDRAVIIQRTQMVTPLVFLAYSTNGKAFLDGLLIYVQLLEEPLESKSQHHLERPLKDMFPRMFVISMAKKVKVNELWNDYSWDLRLRRGMLDREANKWFGLCSTLSQHSFNGNDDVIWWKPENSGIFSVKSALLALQLNRRLLVEELSSQIWEGTIPNRVKFFFWTLALNGINTMDMVQNMCVMCCRNEESASHLLLHCDFAVQVWNRFAESFGQKGCKPRNVVDWLPEILVGGGLKDKARILWKNFSRAIIWHLWKERNARVPFVLVEGPSPYSDFIVVNMDGSRHPLPLTTVYHQLEPVCATPYTFLQALFGHEYPVGVLDEEKILPLGKNISAVGICSFRNGVPVIKSCKDFPHFLCEMTKDQMILDLVFKTKILFWSSIVLGSLSVGILGYAVVRNWNKWKRWTQQGRLQDSGNDSTMSQLDSSNNIPIYGDSGTALDDEVAAVSDDEVLNIPDGQLCVICLMRRKRSAFIPCGHLVCCERCAISVERDSAPKCPVCRQQIHRSVRIYDS
Homology
[Close]
BLAST of Spg014522 vs. NCBI nr
Match: XP_022964217.1 (E3 ubiquitin-protein ligase SPL2-like isoform X1 [Cucurbita moschata] >XP_022964218.1 E3 ubiquitin-protein ligase SPL2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 599.7 bits (1545), Expect = 2.8e-167
Identity = 355/623 (56.98%), Postives = 383/623 (61.48%), Query Frame = 0 

Query: 1   MSSSELALVSLVSRFALSFDGAVLGAALAYATVRTVLKLTASCSALKELRKAPYVRVSDL 60
           MSSSE ALVSL SRFALSFDGA LGAALAYA  RTVLKLTASCSAL ELRKAP VRVSDL
Sbjct: 1   MSSSEQALVSLFSRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL 60

Query: 61  RHIISEGDSEQSGSDQKLVVVRGTVEARSSVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120
           RH+IS+GDS QS SDQKLVVVRGTVEARS VEGNWKSLQPNVLISQESGDRAVIIQRTQM
Sbjct: 61  RHMISDGDSRQSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120

Query: 121 VTPLVFLAYSTNGKAFLDGLLIYVQLLEEPLESKSQHHLERPLKDMFPRMFVISMAKKVK 180
                        K FL                                           
Sbjct: 121 -------CIFNEWKGFL------------------------------------------- 180

Query: 181 VNELWNDYSWDLRLRRGMLDREANKWFGLCSTLSQHSFNGNDDVIWWKPENSGIFSVKSA 240
                  ++ DLR   G   R                          K E++   +VK +
Sbjct: 181 ------GWTSDLRAIFGRTFR--------------------------KQESTSFRTVKFS 240

Query: 241 LLALQLNRRLLVEELSSQIWEGTIPNRVKFFFWTLALNGINTMDMVQNMCVMCCRNEESA 300
              L L             W  +  +   FFF  L                         
Sbjct: 241 FSLLLL-------------WSFSCDSYWIFFFPLLVARA--------------------- 300

Query: 301 SHLLLHCDFAVQVWNRFAESFGQKGCKPRNVVDWLPEILVGGGLKDKARILWKNFSRAII 360
                         +RF  +   +   P  V           GLK               
Sbjct: 301 --------------SRFVSAISSER-GPNRV-----------GLK--------------- 360

Query: 361 WHLWKERNARVPFVLVEGPSPYSDFIVVNMDGSRHPLPLTTVYHQLEPVCATPYTFLQAL 420
                     VPFVLVEG SPYSDF VVNMDGSRH LPLTTVY QL+PVCATPYTFLQAL
Sbjct: 361 ----------VPFVLVEGHSPYSDFAVVNMDGSRHRLPLTTVYRQLQPVCATPYTFLQAL 420

Query: 421 FGHEYPVGVLDEEKILPLGKNISAVGICSFRNGVPVIKSCKDFPHFLCEMTKDQMILDLV 480
           FGHEYPVGVLDEEKILPLG+NIS VG+CSF+NGVP IKSCKDFPHFLCEMTKDQM+LDLV
Sbjct: 421 FGHEYPVGVLDEEKILPLGRNISVVGVCSFKNGVPEIKSCKDFPHFLCEMTKDQMVLDLV 456

Query: 481 FKTKILFWSSIVLGSLSVGILGYAVVRNWNKWKRWTQQGRLQDSGNDSTMSQLDSSNNIP 540
           FKTK LFWSSIVLGSLSVG+LG +V+RNWN+WK+W QQ RLQDSGNDSTMSQLDSSNNIP
Sbjct: 481 FKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWRQQRRLQDSGNDSTMSQLDSSNNIP 456

Query: 541 IYGDSGTALDDEVAAVSDDEV-LNIPDGQLCVICLMRRKRSAFIPCGHLVCCERCAISVE 600
           IYGD GTA DDEVA++ DDE+  N+PDGQLCVICLMRR+RSAFIPCGHLVCCERCAISVE
Sbjct: 541 IYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLMRRRRSAFIPCGHLVCCERCAISVE 456

Query: 601 RDSAPKCPVCRQQIHRSVRIYDS 623
           RDSAPKCPVCRQQI  SVRIYDS
Sbjct: 601 RDSAPKCPVCRQQIRSSVRIYDS 456

[Close]
BLAST of Spg014522 vs. NCBI nr
Match: XP_023000258.1 (E3 ubiquitin-protein ligase SPL2-like isoform X1 [Cucurbita maxima] >XP_023000259.1 E3 ubiquitin-protein ligase SPL2-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 597.0 bits (1538), Expect = 1.8e-166
Identity = 353/624 (56.57%), Postives = 385/624 (61.70%), Query Frame = 0 

Query: 1   MSSSELALVSLVSRFALSFDGAVLGAALAYATVRTVLKLTASCSALKELRKAPYVRVSDL 60
           MSSSE ALVSL SRFALSFDGA LGAALAYA  RTVLKLTASCSAL ELRKAP VRVSDL
Sbjct: 1   MSSSEQALVSLFSRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL 60

Query: 61  RHIISEGDSEQSGSDQKLVVVRGTVEARSSVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120
           RH+ S+GDS QS SDQKLVVVRGTVEARS VEGNWKSLQPNVLISQESGDRAVIIQRTQM
Sbjct: 61  RHMTSDGDSGQSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120

Query: 121 VTPLVFLAYSTNGKAFLDGLLIYVQLLEEPLESKSQHHLERPLKDMFPRMFVISMAKKVK 180
                        K FL                                           
Sbjct: 121 -------CIFNEWKGFL------------------------------------------- 180

Query: 181 VNELWNDYSWDLRLRRGMLDREANKWFGLCSTLSQHSFNGNDDVIWWKPENSGIFSVKSA 240
                  ++ DLR   G   R                          K E++   +VKS+
Sbjct: 181 ------GWTSDLRAIFGRTFR--------------------------KQESTSFRTVKSS 240

Query: 241 LLALQLNRRLLVEELSSQIWEGTIPNRVKFFFWTLALNGINTMDMVQNMCVMCCRNEESA 300
              L L                          W+ +                        
Sbjct: 241 FSLLLL--------------------------WSFS------------------------ 300

Query: 301 SHLLLHCDFAVQVWNRFAESFGQKGCKPRNVVDWLPEILVGGGLKDKARILWKNFSRAII 360
                 CD                     +++D L  +LV              F  AI 
Sbjct: 301 ------CD---------------------SLLDLLFPLLVARA---------SRFVSAIS 360

Query: 361 WHLWKER-NARVPFVLVEGPSPYSDFIVVNMDGSRHPLPLTTVYHQLEPVCATPYTFLQA 420
                 R   +VPFVLVEG SPYSDF+VVNMDGSRH LPLTTVY QL+PVCATPYTFLQA
Sbjct: 361 SERGPNRVGLKVPFVLVEGHSPYSDFVVVNMDGSRHRLPLTTVYRQLQPVCATPYTFLQA 420

Query: 421 LFGHEYPVGVLDEEKILPLGKNISAVGICSFRNGVPVIKSCKDFPHFLCEMTKDQMILDL 480
           LFGH+YPVGVLDEEKILPLGKNIS VG+CSF+NGVP IKSCKDFPHFLCEMTKDQM+LDL
Sbjct: 421 LFGHKYPVGVLDEEKILPLGKNISVVGVCSFKNGVPEIKSCKDFPHFLCEMTKDQMVLDL 456

Query: 481 VFKTKILFWSSIVLGSLSVGILGYAVVRNWNKWKRWTQQGRLQDSGNDSTMSQLDSSNNI 540
           VFKTK LFWSSIVLGSLSVG+LG +V+RNWN+WK+W QQ RLQDSGNDST+SQLDSSNNI
Sbjct: 481 VFKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWRQQRRLQDSGNDSTISQLDSSNNI 456

Query: 541 PIYGDSGTALDDEVAAVSDDEV-LNIPDGQLCVICLMRRKRSAFIPCGHLVCCERCAISV 600
           PIYGD GTA DDEVA++ DDE+  N+PDGQLCVICLMRR+RSAFIPCGHLVCCERCAISV
Sbjct: 541 PIYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLMRRRRSAFIPCGHLVCCERCAISV 456

Query: 601 ERDSAPKCPVCRQQIHRSVRIYDS 623
           ERDSAPKCPVCRQQI  SVRIYDS
Sbjct: 601 ERDSAPKCPVCRQQIRSSVRIYDS 456

[Close]
BLAST of Spg014522 vs. NCBI nr
Match: XP_023514151.1 (E3 ubiquitin-protein ligase SPL2-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 594.0 bits (1530), Expect = 1.5e-165
Identity = 352/624 (56.41%), Postives = 383/624 (61.38%), Query Frame = 0 

Query: 1   MSSSELALVSLVSRFALSFDGAVLGAALAYATVRTVLKLTASCSALKELRKAPYVRVSDL 60
           MSSSE ALVSL+SRFALSFDGA LGAALAYA  RTVLKLTASCSAL ELRKAP VRVSDL
Sbjct: 1   MSSSEQALVSLLSRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL 60

Query: 61  RHIISEGDSEQSGSDQKLVVVRGTVEARSSVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120
           RH+IS+GDS QS SDQKLVVVRGTVEARS VEGNWKSLQPNVLISQESGDRAVIIQRTQM
Sbjct: 61  RHMISDGDSRQSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120

Query: 121 VTPLVFLAYSTNGKAFLDGLLIYVQLLEEPLESKSQHHLERPLKDMFPRMFVISMAKKVK 180
               +F                                                      
Sbjct: 121 ---CIF------------------------------------------------------ 180

Query: 181 VNELWNDYSWDLRLRRGMLDREANKWFGLCSTLSQHSFNGNDDVIWWKPENSGIFSVKSA 240
                                  N+W G                + W  +   IF     
Sbjct: 181 -----------------------NEWKGF---------------LGWTSDLRAIFG---- 240

Query: 241 LLALQLNRRLLVEELSSQIWEGTIPNRVKFFFWTLALNGINTMDMVQNMCVMCCRNEESA 300
                          + +  E T    VKF F  L L   +                   
Sbjct: 241 --------------RTFRKQESTSFRTVKFSFSLLLLWSFS------------------- 300

Query: 301 SHLLLHCDFAVQVWNRFAESFGQKGCKPRNVVDWLPEILVGGGLKDKARILWKNFSRAII 360
                 CD                 C    ++D L  +LV              F  AI 
Sbjct: 301 ------CD-----------------C----LLDLLFPLLVARA---------SRFVSAIS 360

Query: 361 WHLWKER-NARVPFVLVEGPSPYSDFIVVNMDGSRHPLPLTTVYHQLEPVCATPYTFLQA 420
                 R   +VPFVLVEG SPYSDF+VVNMDGSRH LPLTTVY QL+PVCATPYTFLQA
Sbjct: 361 SERGPNRVGLKVPFVLVEGHSPYSDFVVVNMDGSRHRLPLTTVYRQLQPVCATPYTFLQA 420

Query: 421 LFGHEYPVGVLDEEKILPLGKNISAVGICSFRNGVPVIKSCKDFPHFLCEMTKDQMILDL 480
           LFGHEYPVGVLDEEKILPLG+NIS VG+CSF+NGVP IKSCKDFPHFLCEMTKDQM+LDL
Sbjct: 421 LFGHEYPVGVLDEEKILPLGRNISVVGVCSFKNGVPEIKSCKDFPHFLCEMTKDQMVLDL 456

Query: 481 VFKTKILFWSSIVLGSLSVGILGYAVVRNWNKWKRWTQQGRLQDSGNDSTMSQLDSSNNI 540
           VFKTK LFWSSIVLGSLSVG+LG +V+RNWN+WK+W QQ RLQDSGNDST+SQLDSSNN 
Sbjct: 481 VFKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWRQQRRLQDSGNDSTISQLDSSNND 456

Query: 541 PIYGDSGTALDDEVAAVSDDEV-LNIPDGQLCVICLMRRKRSAFIPCGHLVCCERCAISV 600
           PIYGD GTA DDE A++ DDE+  N+PDGQLCVICLMRR+RSAFIPCGHLVCCERCAISV
Sbjct: 541 PIYGDFGTASDDEAASIPDDELSSNVPDGQLCVICLMRRRRSAFIPCGHLVCCERCAISV 456

Query: 601 ERDSAPKCPVCRQQIHRSVRIYDS 623
           ERDSAPKCPVCRQQI  SVRIYDS
Sbjct: 601 ERDSAPKCPVCRQQIRSSVRIYDS 456

[Close]
BLAST of Spg014522 vs. NCBI nr
Match: KAG7013989.1 (E3 ubiquitin-protein ligase SPL2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 578.6 bits (1490), Expect = 6.7e-161
Identity = 348/624 (55.77%), Postives = 367/624 (58.81%), Query Frame = 0 

Query: 1   MSSSELALVSLVSRFALSFDGAVLGAALAYATVRTVLKLTASCSALKELRKAPYVRVSDL 60
           MSSSE ALVSL+S+FALSFDGAVLGAALAYA VRTVLKLTASCSAL ELRKAPYV VSDL
Sbjct: 1   MSSSEQALVSLLSQFALSFDGAVLGAALAYAAVRTVLKLTASCSALDELRKAPYVSVSDL 60

Query: 61  RHIISEGDSEQSGSDQKLVVVRGTVEARSSVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120
           RHIIS+GDS QS SD KLVVVRGTVEARSSVEGNWKS+QPNVLISQESGDRAVIIQRTQM
Sbjct: 61  RHIISDGDSGQSESDHKLVVVRGTVEARSSVEGNWKSVQPNVLISQESGDRAVIIQRTQM 120

Query: 121 VTPLVFLAYSTNGKAFLDGLLIYVQLLEEPLESKSQHHLERPLKDMFPRMFVISMAKKVK 180
               +F                                                      
Sbjct: 121 ---CIF------------------------------------------------------ 180

Query: 181 VNELWNDYSWDLRLRRGMLDREANKWFGLCSTLSQHSFNGNDDVIWWKPENSGIFSVKSA 240
                                  N+W G                                
Sbjct: 181 -----------------------NEWKG-------------------------------- 240

Query: 241 LLALQLNRRLLVEELSSQIWEGTIPNRVKFFFWTLALNGINTMDMVQNMCVMCCRNEESA 300
                                        FF WT  L  I              R +ES 
Sbjct: 241 -----------------------------FFGWTSDLRAIFGRTF---------RKQEST 300

Query: 301 SHLLLHCDFAVQVWNRFAESFGQKGCKPRNVVDWLPEILVGGGLKDKARILWKNFSRAII 360
           S                                                           
Sbjct: 301 S----------------------------------------------------------- 360

Query: 361 WHLWKERNARVPFVLV-EGPSPYSDFIVVNMDGSRHPLPLTTVYHQLEPVCATPYTFLQA 420
                     VPFVLV EG SPYSDF+VVNMDGSRHPLPLTTVYHQL+PV ATPYTFLQA
Sbjct: 361 -------LRTVPFVLVDEGHSPYSDFVVVNMDGSRHPLPLTTVYHQLQPVSATPYTFLQA 407

Query: 421 LFGHEYPVGVLDEEKILPLGKNISAVGICSFRNGVPVIKSCKDFPHFLCEMTKDQMILDL 480
           LFGHEYPVGVLDEEKILPLGKNISAVGICSF+NGVPVIKSCKDFPHFLCEMTKDQMILDL
Sbjct: 421 LFGHEYPVGVLDEEKILPLGKNISAVGICSFKNGVPVIKSCKDFPHFLCEMTKDQMILDL 407

Query: 481 VFKTKILFWSSIVLGSLSVGILGYAVVRNWNKWKRWTQQGRLQDSGNDSTMSQLDSSNNI 540
           VFKTK LFWSS+VLGSLSVG+LGY+VVRNWN+WK+W QQ RLQDSGNDSTMSQLD SNNI
Sbjct: 481 VFKTKFLFWSSVVLGSLSVGVLGYSVVRNWNRWKQWRQQRRLQDSGNDSTMSQLD-SNNI 407

Query: 541 PIYGDSGTALDDEVAAVSDDEV-LNIPDGQLCVICLMRRKRSAFIPCGHLVCCERCAISV 600
           PIYGD GTA D+E AA  +DEV  N+PDGQLCVICLMRRKRSAFIPCGH VCCERCA+SV
Sbjct: 541 PIYGDFGTASDNEEAADPEDEVSSNVPDGQLCVICLMRRKRSAFIPCGHFVCCERCAMSV 407

Query: 601 ERDSAPKCPVCRQQIHRSVRIYDS 623
           ERDSAPKCPVCRQQI  SVRIYDS
Sbjct: 601 ERDSAPKCPVCRQQIRSSVRIYDS 407

[Close]
BLAST of Spg014522 vs. NCBI nr
Match: XP_022964220.1 (E3 ubiquitin-protein ligase SPL2-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 576.6 bits (1485), Expect = 2.5e-160
Identity = 342/623 (54.90%), Postives = 362/623 (58.11%), Query Frame = 0 

Query: 1   MSSSELALVSLVSRFALSFDGAVLGAALAYATVRTVLKLTASCSALKELRKAPYVRVSDL 60
           MSSSE ALVSL SRFALSFDGA LGAALAYA  RTVLKLTASCSAL ELRKAP VRVSDL
Sbjct: 1   MSSSEQALVSLFSRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL 60

Query: 61  RHIISEGDSEQSGSDQKLVVVRGTVEARSSVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120
           RH+IS+GDS QS SDQKLVVVRGTVEARS VEGNWKSLQPNVLISQESGDRAVIIQRTQM
Sbjct: 61  RHMISDGDSRQSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120

Query: 121 VTPLVFLAYSTNGKAFLDGLLIYVQLLEEPLESKSQHHLERPLKDMFPRMFVISMAKKVK 180
               +F                                                      
Sbjct: 121 ---CIF------------------------------------------------------ 180

Query: 181 VNELWNDYSWDLRLRRGMLDREANKWFGLCSTLSQHSFNGNDDVIWWKPENSGIFSVKSA 240
                                  N+W G                                
Sbjct: 181 -----------------------NEWKG-------------------------------- 240

Query: 241 LLALQLNRRLLVEELSSQIWEGTIPNRVKFFFWTLALNGINTMDMVQNMCVMCCRNEESA 300
                                        F  WT  L  I              R +ES 
Sbjct: 241 -----------------------------FLGWTSDLRAIFGRTF---------RKQEST 300

Query: 301 SHLLLHCDFAVQVWNRFAESFGQKGCKPRNVVDWLPEILVGGGLKDKARILWKNFSRAII 360
           S                                                           
Sbjct: 301 SF---------------------------------------------------------- 360

Query: 361 WHLWKERNARVPFVLVEGPSPYSDFIVVNMDGSRHPLPLTTVYHQLEPVCATPYTFLQAL 420
                     VPFVLVEG SPYSDF VVNMDGSRH LPLTTVY QL+PVCATPYTFLQAL
Sbjct: 361 --------RTVPFVLVEGHSPYSDFAVVNMDGSRHRLPLTTVYRQLQPVCATPYTFLQAL 407

Query: 421 FGHEYPVGVLDEEKILPLGKNISAVGICSFRNGVPVIKSCKDFPHFLCEMTKDQMILDLV 480
           FGHEYPVGVLDEEKILPLG+NIS VG+CSF+NGVP IKSCKDFPHFLCEMTKDQM+LDLV
Sbjct: 421 FGHEYPVGVLDEEKILPLGRNISVVGVCSFKNGVPEIKSCKDFPHFLCEMTKDQMVLDLV 407

Query: 481 FKTKILFWSSIVLGSLSVGILGYAVVRNWNKWKRWTQQGRLQDSGNDSTMSQLDSSNNIP 540
           FKTK LFWSSIVLGSLSVG+LG +V+RNWN+WK+W QQ RLQDSGNDSTMSQLDSSNNIP
Sbjct: 481 FKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWRQQRRLQDSGNDSTMSQLDSSNNIP 407

Query: 541 IYGDSGTALDDEVAAVSDDEV-LNIPDGQLCVICLMRRKRSAFIPCGHLVCCERCAISVE 600
           IYGD GTA DDEVA++ DDE+  N+PDGQLCVICLMRR+RSAFIPCGHLVCCERCAISVE
Sbjct: 541 IYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLMRRRRSAFIPCGHLVCCERCAISVE 407

Query: 601 RDSAPKCPVCRQQIHRSVRIYDS 623
           RDSAPKCPVCRQQI  SVRIYDS
Sbjct: 601 RDSAPKCPVCRQQIRSSVRIYDS 407

[Close]
BLAST of Spg014522 vs. ExPASy Swiss-Prot
Match: Q9SYH3 (E3 ubiquitin-protein ligase SPL2 OS=Arabidopsis thaliana OX=3702 GN=SPL2 PE=2 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 3.5e-72
Identity = 203/629 (32.27%), Postives = 275/629 (43.72%), Query Frame = 0 

Query: 1   MSSSELALVSLVSRFALSFDGAVLGAALAYATVRTVLKLTASCSALKELRKAPYVRVSDL 60
           MSS E AL++L++  ALSFDGA+LG  LA + V + LK  ++ +ALK+++ AP V +SDL
Sbjct: 1   MSSPERALLNLLTDIALSFDGAILGLTLAVSAVGSALKYASTNAALKKIKDAPEVSISDL 60

Query: 61  RHII------SEGDSEQSGSDQKLVVVRGTVEARSSVEGNWKSLQPNVLISQESGDRAVI 120
           R ++      SE +  +  +DQ++VVVRG V+ + S +  +K+   NVLIS E+GD+A+I
Sbjct: 61  RSLLPASEDKSETNDNRKSNDQRIVVVRGVVKPKISGDEGYKN--NNVLISPETGDKALI 120

Query: 121 IQRTQMVTPLVFLAYSTNGKAFLDGLLIYVQLLEEPLESKSQHHLERPLKDMFPRMFVIS 180
           IQRTQ                                                       
Sbjct: 121 IQRTQ------------------------------------------------------- 180

Query: 181 MAKKVKVNELWNDYSWDLRLRRGMLDREANKWFGLCSTLSQHSFNGNDDVIWWKPENSGI 240
                                                                       
Sbjct: 181 ------------------------------------------------------------ 240

Query: 241 FSVKSALLALQLNRRLLVEELSSQIWEGTIPNRVKFFFWTLALNGINTMDMVQNMCVMCC 300
                                 + ++ G                                
Sbjct: 241 ----------------------TYVYSG-------------------------------- 300

Query: 301 RNEESASHLLLHCDFAVQVWNRFAESFGQKGCKPRNVVDWLPEILVGGGLKDKARILWKN 360
                              W R  +S G +    R++                 R    +
Sbjct: 301 -------------------WKRLFQSTGHRFMLERSL-----------------RKHGAD 360

Query: 361 FSRAIIWHLWKERNARVPFVLV-EGPSPYSDFIVVNMDGSRHPLPLTTVYHQLEPVCATP 420
           F+R             VPFV+V +     S F+ VNMDGSR PLPLTTVY++L+P+ +  
Sbjct: 361 FTRT------------VPFVIVGKDQQSNSSFVAVNMDGSRQPLPLTTVYNRLQPINS-- 383

Query: 421 YTFLQALFGHEYPVGVLDEEKILPLGKNISAVGICSFRNGVPVIKSCKDFPHFLCEMTKD 480
            +FLQA    +YPVG+LD EKILP GK+I+AVGI SF NGVP IKSC+D P+FL EMTKD
Sbjct: 421 -SFLQAFLYPDYPVGLLDIEKILPPGKDITAVGIYSFNNGVPEIKSCQDLPYFLSEMTKD 383

Query: 481 QMILDLVFKTKILFWSSIVLGSLSVGILGYAVVRNWNKWKRWTQQGRLQDSGNDSTMSQL 540
           +MI DL+ +T  +F  S++LG +SVGIL YA VR WNKWK+W  Q  L    NDS     
Sbjct: 481 KMIEDLMEQTNFIFLGSVILGIVSVGILSYAAVRTWNKWKQWNHQRELPQRPNDS----- 383

Query: 541 DSSNNIPIYGDSGTALDDEVAAVSDDEVLNIPDGQLCVICLMRRKRSAFIPCGHLVCCER 600
                          +DDE      ++   IPDG+LCVIC+ RR+  AFIPCGH+VCC R
Sbjct: 541 --------------VVDDE-----PEDADEIPDGELCVICVSRRRVPAFIPCGHVVCCRR 383

Query: 601 CAISVERDSAPKCPVCRQQIHRSVRIYDS 623
           CA +VER+  PKCPVC Q I  S+R+Y S
Sbjct: 601 CASTVERELNPKCPVCLQSIRGSMRVYYS 383

[Close]
BLAST of Spg014522 vs. ExPASy Swiss-Prot
Match: Q94HV7 (E3 ubiquitin-protein ligase SPL1 OS=Arabidopsis thaliana OX=3702 GN=SPL1 PE=2 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 3.2e-09
Identity = 67/268 (25.00%), Postives = 124/268 (46.27%), Query Frame = 0 

Query: 363 LWKERNARVPFVLVEGPSPYSDFIVVNMDGSRHPLPL-----TTVYHQLEPVCATPYTFL 422
           L +     VP+ L +G         VN+D S+  L L     + V+ + EPV     + +
Sbjct: 101 LMQPMTKEVPWYLDDGTGR------VNVDVSQGELGLALTVGSDVFEKAEPV-----SLV 160

Query: 423 QALFGHEYP---VGVLDEEKILPLGKNISAVGICSFRNGVPVIKSCK--DFPHFLCEMTK 482
           Q   G+      +GV   E+++P+G  ++ VG  + R+G+  ++  K    P ++  +  
Sbjct: 161 QGALGYLKGFKILGVRHVERVVPIGTPLTVVGE-AVRDGMGNVRIQKPEQGPFYVTYIPL 220

Query: 483 DQMILDLVFKTKILFWSSIVLGSLSVGILGYAVVRNWNKWKRWTQQGRLQDSGNDSTMSQ 542
           DQ+I  L   ++   ++S+ L  L V ++   V+    K    T + R +       +  
Sbjct: 221 DQLISKLGDLSRRFKYASMGLTVLGVILISKPVIEYILKRIEDTLERRRRQFALKRVVDA 280

Query: 543 LDSSNNIPIYGDSGTALDDEVAAVSDDEVLNIPDGQLCVICLMRRKRSAFIPCGHLVCCE 602
             +    P+ G  GT+ D +            PD  LCV+CL ++  +AF+ CGH+ CC 
Sbjct: 281 A-ARRAKPVTG-GGTSRDGDT-----------PD--LCVVCLDQKYNTAFVECGHMCCCT 336

Query: 603 RCAISVERDSAPKCPVCRQQIHRSVRIY 621
            C++ +       CP+CR++I + ++IY
Sbjct: 341 PCSLQLR-----TCPLCRERIQQVLKIY 336

[Close]
BLAST of Spg014522 vs. ExPASy Swiss-Prot
Match: P41437 (E3 ubiquitin-protein ligase IAP-3 OS=Orgyia pseudotsugata multicapsid polyhedrosis virus OX=262177 GN=IAP3 PE=3 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 2.3e-07
Identity = 26/57 (45.61%), Postives = 35/57 (61.40%), Query Frame = 0 

Query: 564 IPDGQLCVICLMRRKRSAFIPCGHLVCCERCAISVERDSAPKCPVCRQQIHRSVRIY 621
           + D +LC ICL   K   F+PCGH+V C +CA  V       CPVCR Q+ ++VR+Y
Sbjct: 215 VADDRLCKICLGAEKTVCFVPCGHVVACGKCAAGV-----TTCPVCRGQLDKAVRMY 266

[Close]
BLAST of Spg014522 vs. ExPASy Swiss-Prot
Match: O08863 (Baculoviral IAP repeat-containing protein 3 OS=Mus musculus OX=10090 GN=Birc3 PE=1 SV=2)

HSP 1 Score: 56.6 bits (135), Expect = 1.2e-06
Identity = 26/71 (36.62%), Postives = 41/71 (57.75%), Query Frame = 0 

Query: 550 DDEVAAVSDDEVLNIPDGQLCVICLMRRKRSAFIPCGHLVCCERCAISVERDSAPKCPVC 609
           DD  A   ++++  + + ++C +C+ R     FIPCGHLV C+ CA S+      KCP+C
Sbjct: 533 DDIAALPMEEQLRKLQEERMCKVCMDREVSIVFIPCGHLVVCKDCAPSLR-----KCPIC 592

Query: 610 RQQIHRSVRIY 621
           R  I  +VR +
Sbjct: 593 RGTIKGTVRTF 598

[Close]
BLAST of Spg014522 vs. ExPASy Swiss-Prot
Match: Q24306 (Death-associated inhibitor of apoptosis 1 OS=Drosophila melanogaster OX=7227 GN=Diap1 PE=1 SV=2)

HSP 1 Score: 56.6 bits (135), Expect = 1.2e-06
Identity = 26/60 (43.33%), Postives = 37/60 (61.67%), Query Frame = 0 

Query: 563 NIPDGQLCVICLMRRKRSAFIPCGHLVCCERCAISVERDSAPKCPVCRQQIHRSVRIYDS 622
           +IP+ +LC IC      +AF+PCGH+V C +CA SV      KCP+CR+     +R+Y S
Sbjct: 384 SIPEEKLCKICYGAEYNTAFLPCGHVVACAKCASSV-----TKCPLCRKPFTDVMRVYFS 438

[Close]
BLAST of Spg014522 vs. ExPASy TrEMBL
Match: A0A6J1HH89 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111464304 PE=4 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 1.3e-167
Identity = 355/623 (56.98%), Postives = 383/623 (61.48%), Query Frame = 0 

Query: 1   MSSSELALVSLVSRFALSFDGAVLGAALAYATVRTVLKLTASCSALKELRKAPYVRVSDL 60
           MSSSE ALVSL SRFALSFDGA LGAALAYA  RTVLKLTASCSAL ELRKAP VRVSDL
Sbjct: 1   MSSSEQALVSLFSRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL 60

Query: 61  RHIISEGDSEQSGSDQKLVVVRGTVEARSSVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120
           RH+IS+GDS QS SDQKLVVVRGTVEARS VEGNWKSLQPNVLISQESGDRAVIIQRTQM
Sbjct: 61  RHMISDGDSRQSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120

Query: 121 VTPLVFLAYSTNGKAFLDGLLIYVQLLEEPLESKSQHHLERPLKDMFPRMFVISMAKKVK 180
                        K FL                                           
Sbjct: 121 -------CIFNEWKGFL------------------------------------------- 180

Query: 181 VNELWNDYSWDLRLRRGMLDREANKWFGLCSTLSQHSFNGNDDVIWWKPENSGIFSVKSA 240
                  ++ DLR   G   R                          K E++   +VK +
Sbjct: 181 ------GWTSDLRAIFGRTFR--------------------------KQESTSFRTVKFS 240

Query: 241 LLALQLNRRLLVEELSSQIWEGTIPNRVKFFFWTLALNGINTMDMVQNMCVMCCRNEESA 300
              L L             W  +  +   FFF  L                         
Sbjct: 241 FSLLLL-------------WSFSCDSYWIFFFPLLVARA--------------------- 300

Query: 301 SHLLLHCDFAVQVWNRFAESFGQKGCKPRNVVDWLPEILVGGGLKDKARILWKNFSRAII 360
                         +RF  +   +   P  V           GLK               
Sbjct: 301 --------------SRFVSAISSER-GPNRV-----------GLK--------------- 360

Query: 361 WHLWKERNARVPFVLVEGPSPYSDFIVVNMDGSRHPLPLTTVYHQLEPVCATPYTFLQAL 420
                     VPFVLVEG SPYSDF VVNMDGSRH LPLTTVY QL+PVCATPYTFLQAL
Sbjct: 361 ----------VPFVLVEGHSPYSDFAVVNMDGSRHRLPLTTVYRQLQPVCATPYTFLQAL 420

Query: 421 FGHEYPVGVLDEEKILPLGKNISAVGICSFRNGVPVIKSCKDFPHFLCEMTKDQMILDLV 480
           FGHEYPVGVLDEEKILPLG+NIS VG+CSF+NGVP IKSCKDFPHFLCEMTKDQM+LDLV
Sbjct: 421 FGHEYPVGVLDEEKILPLGRNISVVGVCSFKNGVPEIKSCKDFPHFLCEMTKDQMVLDLV 456

Query: 481 FKTKILFWSSIVLGSLSVGILGYAVVRNWNKWKRWTQQGRLQDSGNDSTMSQLDSSNNIP 540
           FKTK LFWSSIVLGSLSVG+LG +V+RNWN+WK+W QQ RLQDSGNDSTMSQLDSSNNIP
Sbjct: 481 FKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWRQQRRLQDSGNDSTMSQLDSSNNIP 456

Query: 541 IYGDSGTALDDEVAAVSDDEV-LNIPDGQLCVICLMRRKRSAFIPCGHLVCCERCAISVE 600
           IYGD GTA DDEVA++ DDE+  N+PDGQLCVICLMRR+RSAFIPCGHLVCCERCAISVE
Sbjct: 541 IYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLMRRRRSAFIPCGHLVCCERCAISVE 456

Query: 601 RDSAPKCPVCRQQIHRSVRIYDS 623
           RDSAPKCPVCRQQI  SVRIYDS
Sbjct: 601 RDSAPKCPVCRQQIRSSVRIYDS 456

[Close]
BLAST of Spg014522 vs. ExPASy TrEMBL
Match: A0A6J1KJE8 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111494539 PE=4 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 8.7e-167
Identity = 353/624 (56.57%), Postives = 385/624 (61.70%), Query Frame = 0 

Query: 1   MSSSELALVSLVSRFALSFDGAVLGAALAYATVRTVLKLTASCSALKELRKAPYVRVSDL 60
           MSSSE ALVSL SRFALSFDGA LGAALAYA  RTVLKLTASCSAL ELRKAP VRVSDL
Sbjct: 1   MSSSEQALVSLFSRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL 60

Query: 61  RHIISEGDSEQSGSDQKLVVVRGTVEARSSVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120
           RH+ S+GDS QS SDQKLVVVRGTVEARS VEGNWKSLQPNVLISQESGDRAVIIQRTQM
Sbjct: 61  RHMTSDGDSGQSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120

Query: 121 VTPLVFLAYSTNGKAFLDGLLIYVQLLEEPLESKSQHHLERPLKDMFPRMFVISMAKKVK 180
                        K FL                                           
Sbjct: 121 -------CIFNEWKGFL------------------------------------------- 180

Query: 181 VNELWNDYSWDLRLRRGMLDREANKWFGLCSTLSQHSFNGNDDVIWWKPENSGIFSVKSA 240
                  ++ DLR   G   R                          K E++   +VKS+
Sbjct: 181 ------GWTSDLRAIFGRTFR--------------------------KQESTSFRTVKSS 240

Query: 241 LLALQLNRRLLVEELSSQIWEGTIPNRVKFFFWTLALNGINTMDMVQNMCVMCCRNEESA 300
              L L                          W+ +                        
Sbjct: 241 FSLLLL--------------------------WSFS------------------------ 300

Query: 301 SHLLLHCDFAVQVWNRFAESFGQKGCKPRNVVDWLPEILVGGGLKDKARILWKNFSRAII 360
                 CD                     +++D L  +LV              F  AI 
Sbjct: 301 ------CD---------------------SLLDLLFPLLVARA---------SRFVSAIS 360

Query: 361 WHLWKER-NARVPFVLVEGPSPYSDFIVVNMDGSRHPLPLTTVYHQLEPVCATPYTFLQA 420
                 R   +VPFVLVEG SPYSDF+VVNMDGSRH LPLTTVY QL+PVCATPYTFLQA
Sbjct: 361 SERGPNRVGLKVPFVLVEGHSPYSDFVVVNMDGSRHRLPLTTVYRQLQPVCATPYTFLQA 420

Query: 421 LFGHEYPVGVLDEEKILPLGKNISAVGICSFRNGVPVIKSCKDFPHFLCEMTKDQMILDL 480
           LFGH+YPVGVLDEEKILPLGKNIS VG+CSF+NGVP IKSCKDFPHFLCEMTKDQM+LDL
Sbjct: 421 LFGHKYPVGVLDEEKILPLGKNISVVGVCSFKNGVPEIKSCKDFPHFLCEMTKDQMVLDL 456

Query: 481 VFKTKILFWSSIVLGSLSVGILGYAVVRNWNKWKRWTQQGRLQDSGNDSTMSQLDSSNNI 540
           VFKTK LFWSSIVLGSLSVG+LG +V+RNWN+WK+W QQ RLQDSGNDST+SQLDSSNNI
Sbjct: 481 VFKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWRQQRRLQDSGNDSTISQLDSSNNI 456

Query: 541 PIYGDSGTALDDEVAAVSDDEV-LNIPDGQLCVICLMRRKRSAFIPCGHLVCCERCAISV 600
           PIYGD GTA DDEVA++ DDE+  N+PDGQLCVICLMRR+RSAFIPCGHLVCCERCAISV
Sbjct: 541 PIYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLMRRRRSAFIPCGHLVCCERCAISV 456

Query: 601 ERDSAPKCPVCRQQIHRSVRIYDS 623
           ERDSAPKCPVCRQQI  SVRIYDS
Sbjct: 601 ERDSAPKCPVCRQQIRSSVRIYDS 456

[Close]
BLAST of Spg014522 vs. ExPASy TrEMBL
Match: A0A6J1HK84 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111464304 PE=4 SV=1)

HSP 1 Score: 576.6 bits (1485), Expect = 1.2e-160
Identity = 342/623 (54.90%), Postives = 362/623 (58.11%), Query Frame = 0 

Query: 1   MSSSELALVSLVSRFALSFDGAVLGAALAYATVRTVLKLTASCSALKELRKAPYVRVSDL 60
           MSSSE ALVSL SRFALSFDGA LGAALAYA  RTVLKLTASCSAL ELRKAP VRVSDL
Sbjct: 1   MSSSEQALVSLFSRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL 60

Query: 61  RHIISEGDSEQSGSDQKLVVVRGTVEARSSVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120
           RH+IS+GDS QS SDQKLVVVRGTVEARS VEGNWKSLQPNVLISQESGDRAVIIQRTQM
Sbjct: 61  RHMISDGDSRQSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120

Query: 121 VTPLVFLAYSTNGKAFLDGLLIYVQLLEEPLESKSQHHLERPLKDMFPRMFVISMAKKVK 180
               +F                                                      
Sbjct: 121 ---CIF------------------------------------------------------ 180

Query: 181 VNELWNDYSWDLRLRRGMLDREANKWFGLCSTLSQHSFNGNDDVIWWKPENSGIFSVKSA 240
                                  N+W G                                
Sbjct: 181 -----------------------NEWKG-------------------------------- 240

Query: 241 LLALQLNRRLLVEELSSQIWEGTIPNRVKFFFWTLALNGINTMDMVQNMCVMCCRNEESA 300
                                        F  WT  L  I              R +ES 
Sbjct: 241 -----------------------------FLGWTSDLRAIFGRTF---------RKQEST 300

Query: 301 SHLLLHCDFAVQVWNRFAESFGQKGCKPRNVVDWLPEILVGGGLKDKARILWKNFSRAII 360
           S                                                           
Sbjct: 301 SF---------------------------------------------------------- 360

Query: 361 WHLWKERNARVPFVLVEGPSPYSDFIVVNMDGSRHPLPLTTVYHQLEPVCATPYTFLQAL 420
                     VPFVLVEG SPYSDF VVNMDGSRH LPLTTVY QL+PVCATPYTFLQAL
Sbjct: 361 --------RTVPFVLVEGHSPYSDFAVVNMDGSRHRLPLTTVYRQLQPVCATPYTFLQAL 407

Query: 421 FGHEYPVGVLDEEKILPLGKNISAVGICSFRNGVPVIKSCKDFPHFLCEMTKDQMILDLV 480
           FGHEYPVGVLDEEKILPLG+NIS VG+CSF+NGVP IKSCKDFPHFLCEMTKDQM+LDLV
Sbjct: 421 FGHEYPVGVLDEEKILPLGRNISVVGVCSFKNGVPEIKSCKDFPHFLCEMTKDQMVLDLV 407

Query: 481 FKTKILFWSSIVLGSLSVGILGYAVVRNWNKWKRWTQQGRLQDSGNDSTMSQLDSSNNIP 540
           FKTK LFWSSIVLGSLSVG+LG +V+RNWN+WK+W QQ RLQDSGNDSTMSQLDSSNNIP
Sbjct: 481 FKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWRQQRRLQDSGNDSTMSQLDSSNNIP 407

Query: 541 IYGDSGTALDDEVAAVSDDEV-LNIPDGQLCVICLMRRKRSAFIPCGHLVCCERCAISVE 600
           IYGD GTA DDEVA++ DDE+  N+PDGQLCVICLMRR+RSAFIPCGHLVCCERCAISVE
Sbjct: 541 IYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLMRRRRSAFIPCGHLVCCERCAISVE 407

Query: 601 RDSAPKCPVCRQQIHRSVRIYDS 623
           RDSAPKCPVCRQQI  SVRIYDS
Sbjct: 601 RDSAPKCPVCRQQIRSSVRIYDS 407

[Close]
BLAST of Spg014522 vs. ExPASy TrEMBL
Match: A0A6J1GS48 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111456504 PE=4 SV=1)

HSP 1 Score: 576.2 bits (1484), Expect = 1.6e-160
Identity = 347/624 (55.61%), Postives = 366/624 (58.65%), Query Frame = 0 

Query: 1   MSSSELALVSLVSRFALSFDGAVLGAALAYATVRTVLKLTASCSALKELRKAPYVRVSDL 60
           MSSSE ALVSL+S+FALSFDGAVLGAALAYA VRTVLKLTASCSAL ELRKAPYV VSDL
Sbjct: 1   MSSSEQALVSLLSQFALSFDGAVLGAALAYAAVRTVLKLTASCSALDELRKAPYVSVSDL 60

Query: 61  RHIISEGDSEQSGSDQKLVVVRGTVEARSSVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120
           RHIIS+GDS QS SD KLVVVRGTVEARSSVEGNWKS+QPNVLISQESGDRAVIIQRTQM
Sbjct: 61  RHIISDGDSGQSESDHKLVVVRGTVEARSSVEGNWKSVQPNVLISQESGDRAVIIQRTQM 120

Query: 121 VTPLVFLAYSTNGKAFLDGLLIYVQLLEEPLESKSQHHLERPLKDMFPRMFVISMAKKVK 180
               +F                                                      
Sbjct: 121 ---CIF------------------------------------------------------ 180

Query: 181 VNELWNDYSWDLRLRRGMLDREANKWFGLCSTLSQHSFNGNDDVIWWKPENSGIFSVKSA 240
                                  N+W G                                
Sbjct: 181 -----------------------NEWKG-------------------------------- 240

Query: 241 LLALQLNRRLLVEELSSQIWEGTIPNRVKFFFWTLALNGINTMDMVQNMCVMCCRNEESA 300
                                        FF WT  L  I              R +ES 
Sbjct: 241 -----------------------------FFGWTSDLRAIFGRTF---------RKQEST 300

Query: 301 SHLLLHCDFAVQVWNRFAESFGQKGCKPRNVVDWLPEILVGGGLKDKARILWKNFSRAII 360
           S                                                           
Sbjct: 301 S----------------------------------------------------------- 360

Query: 361 WHLWKERNARVPFVLV-EGPSPYSDFIVVNMDGSRHPLPLTTVYHQLEPVCATPYTFLQA 420
                     VPFVLV EG SPYSDF+VVNMDGSRHPLPLTTVYHQL+PV ATPYTFLQA
Sbjct: 361 -------LRTVPFVLVDEGHSPYSDFVVVNMDGSRHPLPLTTVYHQLQPVSATPYTFLQA 407

Query: 421 LFGHEYPVGVLDEEKILPLGKNISAVGICSFRNGVPVIKSCKDFPHFLCEMTKDQMILDL 480
           LFGHEYPVGVLDEEKILPLGK ISAVGICSF+NGVPVIKSCKDFPHFLCEMTKDQMILDL
Sbjct: 421 LFGHEYPVGVLDEEKILPLGKKISAVGICSFKNGVPVIKSCKDFPHFLCEMTKDQMILDL 407

Query: 481 VFKTKILFWSSIVLGSLSVGILGYAVVRNWNKWKRWTQQGRLQDSGNDSTMSQLDSSNNI 540
           VFKTK LFWSS+VLGSLSVG+LGY+VVRNWN+WK+W QQ RLQDSGNDSTMSQLD SNNI
Sbjct: 481 VFKTKFLFWSSVVLGSLSVGVLGYSVVRNWNRWKQWRQQRRLQDSGNDSTMSQLD-SNNI 407

Query: 541 PIYGDSGTALDDEVAAVSDDEV-LNIPDGQLCVICLMRRKRSAFIPCGHLVCCERCAISV 600
           PIYGD GTA D+E AA  +DEV  N+PDGQLCVICLMRRKRSAFIPCGH VCCERCA+SV
Sbjct: 541 PIYGDFGTASDNEEAADPEDEVSSNVPDGQLCVICLMRRKRSAFIPCGHFVCCERCAMSV 407

Query: 601 ERDSAPKCPVCRQQIHRSVRIYDS 623
           ERDSAPKCPVCRQQI  SVRIYDS
Sbjct: 601 ERDSAPKCPVCRQQIRSSVRIYDS 407

[Close]
BLAST of Spg014522 vs. ExPASy TrEMBL
Match: A0A6J1JT39 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111488642 PE=4 SV=1)

HSP 1 Score: 575.9 bits (1483), Expect = 2.1e-160
Identity = 346/624 (55.45%), Postives = 366/624 (58.65%), Query Frame = 0 

Query: 1   MSSSELALVSLVSRFALSFDGAVLGAALAYATVRTVLKLTASCSALKELRKAPYVRVSDL 60
           MSSSE ALVSL+S+FALSFDGAVLGAALAYA VRTVLKLTASCSAL ELRKAPYV VSDL
Sbjct: 1   MSSSEQALVSLLSQFALSFDGAVLGAALAYAAVRTVLKLTASCSALDELRKAPYVSVSDL 60

Query: 61  RHIISEGDSEQSGSDQKLVVVRGTVEARSSVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120
           RHIIS+GDS +S SD KLVVVRGTVEARSSVEGNWKS+QPNVLISQESGDRAVIIQRTQM
Sbjct: 61  RHIISDGDSGKSESDHKLVVVRGTVEARSSVEGNWKSVQPNVLISQESGDRAVIIQRTQM 120

Query: 121 VTPLVFLAYSTNGKAFLDGLLIYVQLLEEPLESKSQHHLERPLKDMFPRMFVISMAKKVK 180
               +F                                                      
Sbjct: 121 ---CIF------------------------------------------------------ 180

Query: 181 VNELWNDYSWDLRLRRGMLDREANKWFGLCSTLSQHSFNGNDDVIWWKPENSGIFSVKSA 240
                                  N+W G                                
Sbjct: 181 -----------------------NEWKG-------------------------------- 240

Query: 241 LLALQLNRRLLVEELSSQIWEGTIPNRVKFFFWTLALNGINTMDMVQNMCVMCCRNEESA 300
                                        FF WT  L  I              R +ES 
Sbjct: 241 -----------------------------FFGWTSDLRAIFGRTF---------RKQEST 300

Query: 301 SHLLLHCDFAVQVWNRFAESFGQKGCKPRNVVDWLPEILVGGGLKDKARILWKNFSRAII 360
           S                                                           
Sbjct: 301 SF---------------------------------------------------------- 360

Query: 361 WHLWKERNARVPFVLV-EGPSPYSDFIVVNMDGSRHPLPLTTVYHQLEPVCATPYTFLQA 420
                     VPFVLV EG SPYSDF+VVNMDGSRHPLPLTTVYHQL+PV ATPYTFLQA
Sbjct: 361 --------RTVPFVLVDEGHSPYSDFVVVNMDGSRHPLPLTTVYHQLQPVSATPYTFLQA 407

Query: 421 LFGHEYPVGVLDEEKILPLGKNISAVGICSFRNGVPVIKSCKDFPHFLCEMTKDQMILDL 480
           LFGHEYPVGVLDEEKILPLGKNISAVGICSF+NGVPVIKSCKDFPHFLCEMTKDQMILDL
Sbjct: 421 LFGHEYPVGVLDEEKILPLGKNISAVGICSFKNGVPVIKSCKDFPHFLCEMTKDQMILDL 407

Query: 481 VFKTKILFWSSIVLGSLSVGILGYAVVRNWNKWKRWTQQGRLQDSGNDSTMSQLDSSNNI 540
           VFKTK LFWSS+VLGSLSVG+LGY+VVRNWN+WK+W QQ RLQDSGNDSTMSQLD SNNI
Sbjct: 481 VFKTKFLFWSSVVLGSLSVGVLGYSVVRNWNRWKQWRQQRRLQDSGNDSTMSQLD-SNNI 407

Query: 541 PIYGDSGTALDDEVAAVSDDEV-LNIPDGQLCVICLMRRKRSAFIPCGHLVCCERCAISV 600
           PIYGD GTA D+E A   +DEV  N+PDGQLCVICLMRRKRSAFIPCGH VCCERCA+SV
Sbjct: 541 PIYGDFGTASDNEEAVDPEDEVSSNVPDGQLCVICLMRRKRSAFIPCGHFVCCERCAMSV 407

Query: 601 ERDSAPKCPVCRQQIHRSVRIYDS 623
           ERDSAPKCPVCRQQI  SVRIYDS
Sbjct: 601 ERDSAPKCPVCRQQIRSSVRIYDS 407

[Close]
BLAST of Spg014522 vs. TAIR 10
Match: AT1G54150.1 (E3 Ubiquitin ligase family protein )

HSP 1 Score: 274.2 bits (700), Expect = 2.5e-73
Identity = 203/629 (32.27%), Postives = 275/629 (43.72%), Query Frame = 0 

Query: 1   MSSSELALVSLVSRFALSFDGAVLGAALAYATVRTVLKLTASCSALKELRKAPYVRVSDL 60
           MSS E AL++L++  ALSFDGA+LG  LA + V + LK  ++ +ALK+++ AP V +SDL
Sbjct: 1   MSSPERALLNLLTDIALSFDGAILGLTLAVSAVGSALKYASTNAALKKIKDAPEVSISDL 60

Query: 61  RHII------SEGDSEQSGSDQKLVVVRGTVEARSSVEGNWKSLQPNVLISQESGDRAVI 120
           R ++      SE +  +  +DQ++VVVRG V+ + S +  +K+   NVLIS E+GD+A+I
Sbjct: 61  RSLLPASEDKSETNDNRKSNDQRIVVVRGVVKPKISGDEGYKN--NNVLISPETGDKALI 120

Query: 121 IQRTQMVTPLVFLAYSTNGKAFLDGLLIYVQLLEEPLESKSQHHLERPLKDMFPRMFVIS 180
           IQRTQ                                                       
Sbjct: 121 IQRTQ------------------------------------------------------- 180

Query: 181 MAKKVKVNELWNDYSWDLRLRRGMLDREANKWFGLCSTLSQHSFNGNDDVIWWKPENSGI 240
                                                                       
Sbjct: 181 ------------------------------------------------------------ 240

Query: 241 FSVKSALLALQLNRRLLVEELSSQIWEGTIPNRVKFFFWTLALNGINTMDMVQNMCVMCC 300
                                 + ++ G                                
Sbjct: 241 ----------------------TYVYSG-------------------------------- 300

Query: 301 RNEESASHLLLHCDFAVQVWNRFAESFGQKGCKPRNVVDWLPEILVGGGLKDKARILWKN 360
                              W R  +S G +    R++                 R    +
Sbjct: 301 -------------------WKRLFQSTGHRFMLERSL-----------------RKHGAD 360

Query: 361 FSRAIIWHLWKERNARVPFVLV-EGPSPYSDFIVVNMDGSRHPLPLTTVYHQLEPVCATP 420
           F+R             VPFV+V +     S F+ VNMDGSR PLPLTTVY++L+P+ +  
Sbjct: 361 FTRT------------VPFVIVGKDQQSNSSFVAVNMDGSRQPLPLTTVYNRLQPINS-- 383

Query: 421 YTFLQALFGHEYPVGVLDEEKILPLGKNISAVGICSFRNGVPVIKSCKDFPHFLCEMTKD 480
            +FLQA    +YPVG+LD EKILP GK+I+AVGI SF NGVP IKSC+D P+FL EMTKD
Sbjct: 421 -SFLQAFLYPDYPVGLLDIEKILPPGKDITAVGIYSFNNGVPEIKSCQDLPYFLSEMTKD 383

Query: 481 QMILDLVFKTKILFWSSIVLGSLSVGILGYAVVRNWNKWKRWTQQGRLQDSGNDSTMSQL 540
           +MI DL+ +T  +F  S++LG +SVGIL YA VR WNKWK+W  Q  L    NDS     
Sbjct: 481 KMIEDLMEQTNFIFLGSVILGIVSVGILSYAAVRTWNKWKQWNHQRELPQRPNDS----- 383

Query: 541 DSSNNIPIYGDSGTALDDEVAAVSDDEVLNIPDGQLCVICLMRRKRSAFIPCGHLVCCER 600
                          +DDE      ++   IPDG+LCVIC+ RR+  AFIPCGH+VCC R
Sbjct: 541 --------------VVDDE-----PEDADEIPDGELCVICVSRRRVPAFIPCGHVVCCRR 383

Query: 601 CAISVERDSAPKCPVCRQQIHRSVRIYDS 623
           CA +VER+  PKCPVC Q I  S+R+Y S
Sbjct: 601 CASTVERELNPKCPVCLQSIRGSMRVYYS 383

[Close]
BLAST of Spg014522 vs. TAIR 10
Match: AT1G59560.1 (E3 Ubiquitin ligase family protein )

HSP 1 Score: 65.1 bits (157), Expect = 2.3e-10
Identity = 67/268 (25.00%), Postives = 124/268 (46.27%), Query Frame = 0 

Query: 363 LWKERNARVPFVLVEGPSPYSDFIVVNMDGSRHPLPL-----TTVYHQLEPVCATPYTFL 422
           L +     VP+ L +G         VN+D S+  L L     + V+ + EPV     + +
Sbjct: 101 LMQPMTKEVPWYLDDGTGR------VNVDVSQGELGLALTVGSDVFEKAEPV-----SLV 160

Query: 423 QALFGHEYP---VGVLDEEKILPLGKNISAVGICSFRNGVPVIKSCK--DFPHFLCEMTK 482
           Q   G+      +GV   E+++P+G  ++ VG  + R+G+  ++  K    P ++  +  
Sbjct: 161 QGALGYLKGFKILGVRHVERVVPIGTPLTVVGE-AVRDGMGNVRIQKPEQGPFYVTYIPL 220

Query: 483 DQMILDLVFKTKILFWSSIVLGSLSVGILGYAVVRNWNKWKRWTQQGRLQDSGNDSTMSQ 542
           DQ+I  L   ++   ++S+ L  L V ++   V+    K    T + R +       +  
Sbjct: 221 DQLISKLGDLSRRFKYASMGLTVLGVILISKPVIEYILKRIEDTLERRRRQFALKRVVDA 280

Query: 543 LDSSNNIPIYGDSGTALDDEVAAVSDDEVLNIPDGQLCVICLMRRKRSAFIPCGHLVCCE 602
             +    P+ G  GT+ D +            PD  LCV+CL ++  +AF+ CGH+ CC 
Sbjct: 281 A-ARRAKPVTG-GGTSRDGDT-----------PD--LCVVCLDQKYNTAFVECGHMCCCT 336

Query: 603 RCAISVERDSAPKCPVCRQQIHRSVRIY 621
            C++ +       CP+CR++I + ++IY
Sbjct: 341 PCSLQLR-----TCPLCRERIQQVLKIY 336

[Close]
BLAST of Spg014522 vs. TAIR 10
Match: AT4G24204.1 (RING/U-box superfamily protein )

HSP 1 Score: 55.1 bits (131), Expect = 2.4e-07
Identity = 25/70 (35.71%), Postives = 38/70 (54.29%), Query Frame = 0 

Query: 550 DDEVAAVSDDEVLNIPDGQLCVICLMRRKRSAFIPCGHLVCCERCAISVERDSAPKCPVC 609
           D E A+ S  +  ++    LCVIC   R+   F+PCGH   C  CA  +  + +  CP+C
Sbjct: 51  DVETASFSSSD--DVDYSTLCVICFEERRNCFFVPCGHSATCRGCAQRILSEESKVCPIC 110

Query: 610 RQQIHRSVRI 620
           R+ I +S R+
Sbjct: 111 RRVIRKSKRL 118

[Close]
BLAST of Spg014522 vs. TAIR 10
Match: AT4G24204.2 (RING/U-box superfamily protein )

HSP 1 Score: 55.1 bits (131), Expect = 2.4e-07
Identity = 25/70 (35.71%), Postives = 38/70 (54.29%), Query Frame = 0 

Query: 550 DDEVAAVSDDEVLNIPDGQLCVICLMRRKRSAFIPCGHLVCCERCAISVERDSAPKCPVC 609
           D E A+ S  +  ++    LCVIC   R+   F+PCGH   C  CA  +  + +  CP+C
Sbjct: 85  DVETASFSSSD--DVDYSTLCVICFEERRNCFFVPCGHSATCRGCAQRILSEESKVCPIC 144

Query: 610 RQQIHRSVRI 620
           R+ I +S R+
Sbjct: 145 RRVIRKSKRL 152

[Close]
BLAST of Spg014522 vs. TAIR 10
Match: AT4G24204.3 (RING/U-box superfamily protein )

HSP 1 Score: 55.1 bits (131), Expect = 2.4e-07
Identity = 25/70 (35.71%), Postives = 38/70 (54.29%), Query Frame = 0 

Query: 550 DDEVAAVSDDEVLNIPDGQLCVICLMRRKRSAFIPCGHLVCCERCAISVERDSAPKCPVC 609
           D E A+ S  +  ++    LCVIC   R+   F+PCGH   C  CA  +  + +  CP+C
Sbjct: 106 DVETASFSSSD--DVDYSTLCVICFEERRNCFFVPCGHSATCRGCAQRILSEESKVCPIC 165

Query: 610 RQQIHRSVRI 620
           R+ I +S R+
Sbjct: 166 RRVIRKSKRL 173

The following BLAST results are available for this feature:
BLAST of Spg014522 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022964217.12.8e-16756.98E3 ubiquitin-protein ligase SPL2-like isoform X1 [Cucurbita moschata] >XP_022964... [more]
XP_023000258.11.8e-16656.57E3 ubiquitin-protein ligase SPL2-like isoform X1 [Cucurbita maxima] >XP_02300025... [more]
XP_023514151.11.5e-16556.41E3 ubiquitin-protein ligase SPL2-like isoform X1 [Cucurbita pepo subsp. pepo][more]
KAG7013989.16.7e-16155.77E3 ubiquitin-protein ligase SPL2 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022964220.12.5e-16054.90E3 ubiquitin-protein ligase SPL2-like isoform X2 [Cucurbita moschata][more]
BLAST of Spg014522 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9SYH33.5e-7232.27E3 ubiquitin-protein ligase SPL2 OS=Arabidopsis thaliana OX=3702 GN=SPL2 PE=2 SV... [more]
Q94HV73.2e-0925.00E3 ubiquitin-protein ligase SPL1 OS=Arabidopsis thaliana OX=3702 GN=SPL1 PE=2 SV... [more]
P414372.3e-0745.61E3 ubiquitin-protein ligase IAP-3 OS=Orgyia pseudotsugata multicapsid polyhedros... [more]
O088631.2e-0636.62Baculoviral IAP repeat-containing protein 3 OS=Mus musculus OX=10090 GN=Birc3 PE... [more]
Q243061.2e-0643.33Death-associated inhibitor of apoptosis 1 OS=Drosophila melanogaster OX=7227 GN=... [more]
BLAST of Spg014522 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1HH891.3e-16756.98RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111464304... [more]
A0A6J1KJE88.7e-16756.57RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111494539 P... [more]
A0A6J1HK841.2e-16054.90RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111464304... [more]
A0A6J1GS481.6e-16055.61RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111456504... [more]
A0A6J1JT392.1e-16055.45RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111488642 P... [more]
BLAST of Spg014522 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G54150.12.5e-7332.27E3 Ubiquitin ligase family protein [more]
AT1G59560.12.3e-1025.00E3 Ubiquitin ligase family protein [more]
AT4G24204.12.4e-0735.71RING/U-box superfamily protein [more]
AT4G24204.22.4e-0735.71RING/U-box superfamily protein [more]
AT4G24204.32.4e-0735.71RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 570..609
e-value: 0.0029
score: 26.8
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 570..610
score: 10.851695
IPR022170E3 Ubiquitin ligase MUL1-likePFAMPF12483GIDEcoord: 363..500
e-value: 1.8E-32
score: 112.0
IPR026960Reverse transcriptase zinc-binding domainPFAMPF13966zf-RVTcoord: 235..314
e-value: 5.4E-12
score: 46.2
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 550..622
e-value: 3.3E-16
score: 60.4
NoneNo IPR availablePFAMPF13920zf-C3HC4_3coord: 568..615
e-value: 1.9E-13
score: 50.0
NoneNo IPR availablePANTHERPTHR47355:SF1E3 UBIQUITIN-PROTEIN LIGASE SPL2coord: 352..622
NoneNo IPR availablePANTHERPTHR47355:SF1E3 UBIQUITIN-PROTEIN LIGASE SPL2coord: 1..121
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 564..616
IPR044247E3 ubiquitin-protein ligase SPL2-likePANTHERPTHR47355E3 UBIQUITIN-PROTEIN LIGASE SPL2coord: 352..622
IPR044247E3 ubiquitin-protein ligase SPL2-likePANTHERPTHR47355E3 UBIQUITIN-PROTEIN LIGASE SPL2coord: 1..121

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg014522.1Spg014522.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006996 organelle organization
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0110165 cellular anatomical entity
molecular_function GO:0004842 ubiquitin-protein transferase activity