Spg014463 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg014463
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Locationscaffold3: 42881531 .. 42885170 (+)
RNA-Seq ExpressionSpg014463
SyntenySpg014463
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCTTCTTCCTAATGCTGTTACAAAAAGCCCTAGAGAGCAGAGGGTGGATTCAGAAATGCAAGAACAGAGACCAAACATGGAGCAAAATCCTAAGATACTAGAAATCTACCAGCTCCAATATTCGGACCTCTTGCTCTTGTCTTCTCCTAACAATTCTTCATCTTCACCGCAAGAGAACGAACGGATGGAATCGGTAACCAAAGCGATTCTTGAAGCCCTAGGCCCCAATGGACCTGGCCTTCTCGCAATCACCGGCGTCCCCAATTCTTCTCCTCTCCGGCGAGCACTACTGCCTCTCGCTCGCAACCTCGCCTTGCTCAATCCTGATCATCGGAAACGGATTCTTAAGGTTTCATTTCTACATTTTTCCTTACTCCCCTGCTCTCTCTCTCTCCCTCTCTTGCTCCCTCCTTTTTTGTGACTATTTAGTGAGCGTTTGAGGAAAATGACTGAGGAATCTGATGTTGACGGTGCGTTTTGAAGTGGAATAGGACGTGGTCTTCGCCCCCCCACCAAAAATCTCGTCATTGTCACTTTGTTGAAAGCATGTCGATTTGTTTTTAATCGTTATCGTTTTCTTTCTGTTGTTCGAAGCTGAAATAGGGCAAGGAATAGTAACAGATCATTGAGAAATGTCTCGAGATAATTTCATAGTTGCATTGATTTTAACTTTCGGCATACGTGGTGACTTTTGGAGATTAAATTTTGATTGAATTCTAGTTGATCATCTGACATTTCTTTGTTTTATGTTCGACTGGAATGATTTAAGTTTCATAACCATTTGGTTTTTGAAAATTATGCTCTATGATGGTTCTGATCTTTCATTTGGTTTTTGAAAATTATGCCCTATGATGGTTATGAACTTTCTTAATGATCCAGTTGAATTCCTAGACAAATTTCAAAAACAAAAACAAGTTTTTGAAAACTATATATATTTCAAAATTTGACTTGGTTTTTTAAAACAAAGAAGATACTGAAACAAAGAAATTTATAGGTGGAAGTAGGTGTGTACTTAGTTAATCTGAAACCAAAAACCACTGGGTTCTTTTTTTTGTTTGGTTTTCATGAGATCTTAGCTAAAGTGCTTGTTCACTATGAACATTTTCCTATAACATCCTTCGAAGCAGTCACATTTAAGTTCAAAGGGGACAATCTTGCTAAAAACTCTGTTGCTTTTCATTTAATTTCTCAGGATCACAACTTAGGGAGTGATGTTCCCTTGAGGAATCCAGAAAGAAGCGTCTCCTCTTTTGCTATGCAACTCAAATACACAGAGAGTAAGCAATTCATGCAGAATAGCCAAAGCCAGAGAGAGGACAAACATTCACCTGGTTCAGAAATCGATCATTTTTGTGATTCAATCAAGAAGGAACTTCAGGAAAATGAATTTAAACATCTTGGTGATGCATTTAAAGAGCTAGGAAGTTGCATGATGGAACTGGGGCTTCACATTGCACGTATATGTGATCGGGAAATCGGAGGCCAAGAGTTAGAACAGAGCTTGTTAGAGTCATGCACTGCAAAAGGCCGTCTCATACACTACCATTCAGCTCTGGATGCCCAACTCTTAAGAAAATCAACCAACTGCAAAGGAACTGCAAGAAACCAAGCTAATTCTAGAAGAAATAAAGAACAAAGCATACACAATAGACAAGAGCCATCAGACAGTAATGGACTATGTCATTCAAGCACAAATCTATGGCAGCAATGGCATTATGACTATGGTATCTTCACAGTCCTAACAACTCCCATGTTTCTTTTGCCATCAAATATGCTCGAAACCGAATCACAAGATCTATGTTGCTATTGCGAGTGTTGTTCTCCCAGTGGACACTTGTATTTGCAGATTTTTGATCCTTGTAAGAATGACATTTTCATGGTTAACGCTCCACCAGAAAGTTTTATCATCCAGGTAGGCGAATCGGCTGATATTATATCACGAGGGAAGCTTCGCTCCACTCTTCACTCTGTGTGCAGACCTTCCAAGCAGGAAGACTTGTGCAGAGAAATGTTTGTTGTATTCTTGCAGCCAGCTTGGAACAAAACGTTTTCGATGTCTGATTATCCCATTGAAAGCTCGATGTTATCTGAAGACAGAAAAGATCTTGTTGACAGGGAGGGAACGGCAATAACTCGAGAAATCCAGAAGATAGTTCCACCATTAGCGTCAAGATTGAAGGAAGGAATGACATTTGCAGAGTTCTCACGTGAAACGACCAAGCAATATTACGGGGGAAATGGTTTGCAATCTAACAGATGATTCAGTCATTTGTAAGTTTTCTCCTTAAAAACCCCTTTTTTATCTTAATCTCCAATCAATCAATAGTTTCACCGGCTTAACTTTAGTTGATTGAGCAATTTTCTTATTGCATGCTCTTCTGGATAAGTTCTACATTTGACTGTTAAGAGTCATGACTTTAGTCTAACATAGTCGATCTTGAAATTTGCTTCTAAAAGATTCAAACCAATTCATCCTTTTGTTGGACCTTCCGTATTTTCCATAGTTTTTTTTTTTTTAAGTTTCTCTCACTCTATTTTACTTATTTATTTCTTTTAAATGAGATTGAGTTCAAAATTTTATCATCAAAACGAAAACTGTTTAGAAAATAACAAAGGGTGAAATTGGTAAAAACTGGTGATAGGAATAACTTTCTAAACTAACTATAACAAAGTCAATAAAGCAGTAGCAGTCCTGAAAAGAAAATGAAACATTGATTACTAGGCATAAAATGCTCCTGTCTTTAGCTGTGAAAGATTGACTATGTACATATATATCTATCTGTAAATGCATGTTGTTAACTTCAGCTCCAAATCCCACATATACATGATACAAATCATGGTGTCATATATTTCTCTGGTTCACACAGGGACAAAAAGGGCCAAGGATACACTTTTTGATCATCCTGCTTGAAAGGGTGTGATTGACTAATGTCTAGAATTCTCTCCTGAAGCAAAGTGATGGTGAAGAAAAAGAAAGCATTCTTATTCATGACTCTGCTACTCTAAAAATAGTCAACAGAGAGAGAAAGAGGTTGATGACATCCAAATAGATAGCCACAGCAGCCCAAATGTAATCATCATAAGAGTAAGTGAGGAGGTTCCCTGTGTCATATATGATGTATCCACAGAAAATGATTGAGGCCAAGCACCCATAGACCATTGTAGAGGTCCTACCCAATGGGAAAAAGAGCTGCAGCCATGAAAATGGAACCAGAGGTTAGAATATCAAAACCCCAGATGGAATATGGCATTTTTGTTTTGTTCTTGACAGAACTAAGAACTTGTCAATGGTGTTTGATTAAATGCCTGAGAGAACAAAGTACTTGACCTGAATCATTCCAAATACAATAAGAATCAGCAGAGCTCCAAACAGGAATGGACCAAGGAAGCTGAATTGAATCCCTCTTCTAGCAGCCCAAAATGTGTACAAAGTTAGACTGATGACTGCCACAGTGGTTAGCATTACAGATTCCAAAATCACTTTCCCTGTGAAGCATTGAGAAAGGTGGGAAAATTGTTGAAGTTTGAGATTATAGAGAGACAAATGAATACTGTTTAGATGATTGATCTGGTATGCAAAACATCTACAATCTGATGAGAGAGCAAGTTCTTTTTGTAACTTTTTTTTGCTTTTTCCC

mRNA sequence

ATGCAAGAACAGAGACCAAACATGGAGCAAAATCCTAAGATACTAGAAATCTACCAGCTCCAATATTCGGACCTCTTGCTCTTGTCTTCTCCTAACAATTCTTCATCTTCACCGCAAGAGAACGAACGGATGGAATCGGTAACCAAAGCGATTCTTGAAGCCCTAGGCCCCAATGGACCTGGCCTTCTCGCAATCACCGGCGTCCCCAATTCTTCTCCTCTCCGGCGAGCACTACTGCCTCTCGCTCGCAACCTCGCCTTGCTCAATCCTGATCATCGGAAACGGATTCTTAAGGATCACAACTTAGGGAGTGATGTTCCCTTGAGGAATCCAGAAAGAAGCGTCTCCTCTTTTGCTATGCAACTCAAATACACAGAGAGTAAGCAATTCATGCAGAATAGCCAAAGCCAGAGAGAGGACAAACATTCACCTGGTTCAGAAATCGATCATTTTTGTGATTCAATCAAGAAGGAACTTCAGGAAAATGAATTTAAACATCTTGGTGATGCATTTAAAGAGCTAGGAAGTTGCATGATGGAACTGGGGCTTCACATTGCACGTATATGTGATCGGGAAATCGGAGGCCAAGAGTTAGAACAGAGCTTGTTAGAGTCATGCACTGCAAAAGGCCGTCTCATACACTACCATTCAGCTCTGGATGCCCAACTCTTAAGAAAATCAACCAACTGCAAAGGAACTGCAAGAAACCAAGCTAATTCTAGAAGAAATAAAGAACAAAGCATACACAATAGACAAGAGCCATCAGACAGTAATGGACTATGTCATTCAAGCACAAATCTATGGCAGCAATGGCATTATGACTATGGTATCTTCACAGTCCTAACAACTCCCATGTTTCTTTTGCCATCAAATATGCTCGAAACCGAATCACAAGATCTATGTTGCTATTGCGAGTGTTGTTCTCCCAGTGGACACTTGTATTTGCAGATTTTTGATCCTTGTAAGAATGACATTTTCATGGTTAACGCTCCACCAGAAAGTTTTATCATCCAGGTAGGCGAATCGGCTGATATTATATCACGAGGGAAGCTTCGCTCCACTCTTCACTCTGTGTGCAGACCTTCCAAGCAGGAAGACTTGTGCAGAGAAATGTTTGTTGTATTCTTGCAGCCAGCTTGGAACAAAACGTTTTCGATGTCTGATTATCCCATTGAAAGCTCGATGTTATCTGAAGACAGAAAAGATCTTGTTGACAGGGAGGGAACGGCAATAACTCGAGAAATCCAGAAGATAGTTCCACCATTAGCGTCAAGATTGAAGGAAGGAATGACATTTGCAGAGTTCTCACGTGAAACGACCAAGCAATATTACGGGGGAAATGGTTTGCAATCTAACAGATGA

Coding sequence (CDS)

ATGCAAGAACAGAGACCAAACATGGAGCAAAATCCTAAGATACTAGAAATCTACCAGCTCCAATATTCGGACCTCTTGCTCTTGTCTTCTCCTAACAATTCTTCATCTTCACCGCAAGAGAACGAACGGATGGAATCGGTAACCAAAGCGATTCTTGAAGCCCTAGGCCCCAATGGACCTGGCCTTCTCGCAATCACCGGCGTCCCCAATTCTTCTCCTCTCCGGCGAGCACTACTGCCTCTCGCTCGCAACCTCGCCTTGCTCAATCCTGATCATCGGAAACGGATTCTTAAGGATCACAACTTAGGGAGTGATGTTCCCTTGAGGAATCCAGAAAGAAGCGTCTCCTCTTTTGCTATGCAACTCAAATACACAGAGAGTAAGCAATTCATGCAGAATAGCCAAAGCCAGAGAGAGGACAAACATTCACCTGGTTCAGAAATCGATCATTTTTGTGATTCAATCAAGAAGGAACTTCAGGAAAATGAATTTAAACATCTTGGTGATGCATTTAAAGAGCTAGGAAGTTGCATGATGGAACTGGGGCTTCACATTGCACGTATATGTGATCGGGAAATCGGAGGCCAAGAGTTAGAACAGAGCTTGTTAGAGTCATGCACTGCAAAAGGCCGTCTCATACACTACCATTCAGCTCTGGATGCCCAACTCTTAAGAAAATCAACCAACTGCAAAGGAACTGCAAGAAACCAAGCTAATTCTAGAAGAAATAAAGAACAAAGCATACACAATAGACAAGAGCCATCAGACAGTAATGGACTATGTCATTCAAGCACAAATCTATGGCAGCAATGGCATTATGACTATGGTATCTTCACAGTCCTAACAACTCCCATGTTTCTTTTGCCATCAAATATGCTCGAAACCGAATCACAAGATCTATGTTGCTATTGCGAGTGTTGTTCTCCCAGTGGACACTTGTATTTGCAGATTTTTGATCCTTGTAAGAATGACATTTTCATGGTTAACGCTCCACCAGAAAGTTTTATCATCCAGGTAGGCGAATCGGCTGATATTATATCACGAGGGAAGCTTCGCTCCACTCTTCACTCTGTGTGCAGACCTTCCAAGCAGGAAGACTTGTGCAGAGAAATGTTTGTTGTATTCTTGCAGCCAGCTTGGAACAAAACGTTTTCGATGTCTGATTATCCCATTGAAAGCTCGATGTTATCTGAAGACAGAAAAGATCTTGTTGACAGGGAGGGAACGGCAATAACTCGAGAAATCCAGAAGATAGTTCCACCATTAGCGTCAAGATTGAAGGAAGGAATGACATTTGCAGAGTTCTCACGTGAAACGACCAAGCAATATTACGGGGGAAATGGTTTGCAATCTAACAGATGA

Protein sequence

MQEQRPNMEQNPKILEIYQLQYSDLLLLSSPNNSSSSPQENERMESVTKAILEALGPNGPGLLAITGVPNSSPLRRALLPLARNLALLNPDHRKRILKDHNLGSDVPLRNPERSVSSFAMQLKYTESKQFMQNSQSQREDKHSPGSEIDHFCDSIKKELQENEFKHLGDAFKELGSCMMELGLHIARICDREIGGQELEQSLLESCTAKGRLIHYHSALDAQLLRKSTNCKGTARNQANSRRNKEQSIHNRQEPSDSNGLCHSSTNLWQQWHYDYGIFTVLTTPMFLLPSNMLETESQDLCCYCECCSPSGHLYLQIFDPCKNDIFMVNAPPESFIIQVGESADIISRGKLRSTLHSVCRPSKQEDLCREMFVVFLQPAWNKTFSMSDYPIESSMLSEDRKDLVDREGTAITREIQKIVPPLASRLKEGMTFAEFSRETTKQYYGGNGLQSNR
Homology
BLAST of Spg014463 vs. NCBI nr
Match: XP_022959987.1 (uncharacterized protein LOC111460876 [Cucurbita moschata])

HSP 1 Score: 790.4 bits (2040), Expect = 8.1e-225
Identity = 394/446 (88.34%), Postives = 418/446 (93.72%), Query Frame = 0

Query: 8   MEQNPKILEIYQLQYSDLLLLSSPNNSSSSPQENERMESVTKAILEALGPNGPGLLAITG 67
           ME+NP  LEIY+L+YSDL LLS+  +SSSS Q NER+ES+TK+I EALGP+GPGLLAITG
Sbjct: 1   MEENPNKLEIYELRYSDLFLLSTHCHSSSSLQGNERIESITKSIFEALGPSGPGLLAITG 60

Query: 68  VPNSSPLRRALLPLARNLALLNPDHRKRILKDHNLGSDVPLRNPERSVSSFAMQLKYTES 127
           VPNSS LRR LLPLAR LALLNPD RKRILKDHNLGSDVPLRNPERSVSSFAMQLKYTES
Sbjct: 61  VPNSSVLRRTLLPLARKLALLNPDDRKRILKDHNLGSDVPLRNPERSVSSFAMQLKYTES 120

Query: 128 KQFMQNSQSQREDKHSPGSEIDHFCDSIKKELQENEFKHLGDAFKELGSCMMELGLHIAR 187
           K+FMQN+QSQR DK SPGSEIDHFCDSI+KE+Q+NEFKHLGD+FKELGSCM+ELGLHIAR
Sbjct: 121 KEFMQNNQSQRGDKQSPGSEIDHFCDSIEKEVQDNEFKHLGDSFKELGSCMLELGLHIAR 180

Query: 188 ICDREIGGQELEQSLLESCTAKGRLIHYHSALDAQLLRKSTNCKGTARNQANSRRNKEQS 247
           ICD +IGGQELEQSLLESCTAKGRLIHYHSALDAQLLRKSTNCKGTARNQANSRRNKEQS
Sbjct: 181 ICDAQIGGQELEQSLLESCTAKGRLIHYHSALDAQLLRKSTNCKGTARNQANSRRNKEQS 240

Query: 248 IHNRQEPSDSNGLCHSSTNLWQQWHYDYGIFTVLTTPMFLLPSNMLETESQDLCCYCECC 307
           IH+RQEPSDSNGLC S TNLWQQWHYDYGIFTVLTTPMFL PSN    E+QDLCCY ECC
Sbjct: 241 IHSRQEPSDSNGLCQSITNLWQQWHYDYGIFTVLTTPMFLSPSNTHGNEAQDLCCYSECC 300

Query: 308 SPSGHLYLQIFDPCKNDIFMVNAPPESFIIQVGESADIISRGKLRSTLHSVCRPSKQEDL 367
           SPSGHLYLQIFDPCKNDIFMVNAPPESFIIQVGES+DIISRGKLRSTLHSVCRPSK EDL
Sbjct: 301 SPSGHLYLQIFDPCKNDIFMVNAPPESFIIQVGESSDIISRGKLRSTLHSVCRPSKHEDL 360

Query: 368 CREMFVVFLQPAWNKTFSMSDYPIESSMLSEDRKDLVDREGTAITREIQKIVPPLASRLK 427
           CREMFVVFLQPAWNKTFSMSDY IESS LSED++DLV++E T ITREIQKIVPPLASRLK
Sbjct: 361 CREMFVVFLQPAWNKTFSMSDYSIESSTLSEDKRDLVEQEATVITREIQKIVPPLASRLK 420

Query: 428 EGMTFAEFSRETTKQYYGGNGLQSNR 454
           EGMTFAEFSRETTKQYYGGNGLQSNR
Sbjct: 421 EGMTFAEFSRETTKQYYGGNGLQSNR 446

BLAST of Spg014463 vs. NCBI nr
Match: KAG6593098.1 (hypothetical protein SDJN03_12574, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 789.3 bits (2037), Expect = 1.8e-224
Identity = 394/446 (88.34%), Postives = 418/446 (93.72%), Query Frame = 0

Query: 8   MEQNPKILEIYQLQYSDLLLLSSPNNSSSSPQENERMESVTKAILEALGPNGPGLLAITG 67
           ME+NP  LEIY+L+YSDL LLS+  +SSSS Q NER+ES+TK+I EALGP+GPGLLAITG
Sbjct: 1   MEENPNKLEIYELRYSDLFLLSTHCHSSSSLQGNERIESITKSIFEALGPSGPGLLAITG 60

Query: 68  VPNSSPLRRALLPLARNLALLNPDHRKRILKDHNLGSDVPLRNPERSVSSFAMQLKYTES 127
           VPNSS LRR LLPLAR LALLNPD RKRILKDHNLGSDVPLRNPERSVSSFAMQLKYTES
Sbjct: 61  VPNSSVLRRTLLPLARKLALLNPDDRKRILKDHNLGSDVPLRNPERSVSSFAMQLKYTES 120

Query: 128 KQFMQNSQSQREDKHSPGSEIDHFCDSIKKELQENEFKHLGDAFKELGSCMMELGLHIAR 187
           K+FMQN+QSQR DK SPGSEIDHFCDSI+KE+Q+NEFKHLGD+FKELGSCM+ELGL IAR
Sbjct: 121 KEFMQNNQSQRGDKQSPGSEIDHFCDSIEKEVQDNEFKHLGDSFKELGSCMLELGLRIAR 180

Query: 188 ICDREIGGQELEQSLLESCTAKGRLIHYHSALDAQLLRKSTNCKGTARNQANSRRNKEQS 247
           ICD +IGGQELEQSLLESCTAKGRLIHYHSALDAQLLRKSTNCKGTARNQANSRRNKEQS
Sbjct: 181 ICDAQIGGQELEQSLLESCTAKGRLIHYHSALDAQLLRKSTNCKGTARNQANSRRNKEQS 240

Query: 248 IHNRQEPSDSNGLCHSSTNLWQQWHYDYGIFTVLTTPMFLLPSNMLETESQDLCCYCECC 307
           IH+RQEPSDSNGLC SSTNLWQQWHYDYGIFTVLTTPMFL PSN    E+QDLCCY ECC
Sbjct: 241 IHSRQEPSDSNGLCQSSTNLWQQWHYDYGIFTVLTTPMFLSPSNTHGNEAQDLCCYSECC 300

Query: 308 SPSGHLYLQIFDPCKNDIFMVNAPPESFIIQVGESADIISRGKLRSTLHSVCRPSKQEDL 367
           SPSGHLYLQIFDPCKNDIFMVNAPPESFIIQVGESADIISRGKLRSTLHSVCRPSK EDL
Sbjct: 301 SPSGHLYLQIFDPCKNDIFMVNAPPESFIIQVGESADIISRGKLRSTLHSVCRPSKHEDL 360

Query: 368 CREMFVVFLQPAWNKTFSMSDYPIESSMLSEDRKDLVDREGTAITREIQKIVPPLASRLK 427
           CREMFVVFLQPAWNKTFSMSDY IESS LSED++DLV++E T IT+EIQKIVPPLASRLK
Sbjct: 361 CREMFVVFLQPAWNKTFSMSDYSIESSTLSEDKRDLVEQEATVITQEIQKIVPPLASRLK 420

Query: 428 EGMTFAEFSRETTKQYYGGNGLQSNR 454
           EGMTFAEFSRETTKQYYGGNGLQSNR
Sbjct: 421 EGMTFAEFSRETTKQYYGGNGLQSNR 446

BLAST of Spg014463 vs. NCBI nr
Match: KAG7025506.1 (hypothetical protein SDJN02_12001 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 781.6 bits (2017), Expect = 3.8e-222
Identity = 389/444 (87.61%), Postives = 415/444 (93.47%), Query Frame = 0

Query: 8   MEQNPKILEIYQLQYSDLLLLSSPNNSSSSPQENERMESVTKAILEALGPNGPGLLAITG 67
           ME+NP  LEIY+L+YSDL LLS+  +SSSS Q NER+ES+TK+I EALGP+GPGLLAITG
Sbjct: 1   MEENPNKLEIYELRYSDLFLLSTDCHSSSSLQGNERIESITKSIFEALGPSGPGLLAITG 60

Query: 68  VPNSSPLRRALLPLARNLALLNPDHRKRILKDHNLGSDVPLRNPERSVSSFAMQLKYTES 127
           VPNSS LRR LLPLAR LALLNPD RKRILKDHNLGSDVPLRNPERSVSSFAMQLKYTES
Sbjct: 61  VPNSSVLRRTLLPLARKLALLNPDDRKRILKDHNLGSDVPLRNPERSVSSFAMQLKYTES 120

Query: 128 KQFMQNSQSQREDKHSPGSEIDHFCDSIKKELQENEFKHLGDAFKELGSCMMELGLHIAR 187
           K+FMQN+QSQR DK SPGSEIDHFCDSI+KE+Q+NEFKHLGD+FKELGSCM+ELGL IAR
Sbjct: 121 KEFMQNNQSQRGDKQSPGSEIDHFCDSIEKEVQDNEFKHLGDSFKELGSCMLELGLRIAR 180

Query: 188 ICDREIGGQELEQSLLESCTAKGRLIHYHSALDAQLLRKSTNCKGTARNQANSRRNKEQS 247
           ICD +IGGQELEQSLLESCTAKGRLIHYHSALDAQLLRKSTNCKGTARNQANSRRNKEQS
Sbjct: 181 ICDAQIGGQELEQSLLESCTAKGRLIHYHSALDAQLLRKSTNCKGTARNQANSRRNKEQS 240

Query: 248 IHNRQEPSDSNGLCHSSTNLWQQWHYDYGIFTVLTTPMFLLPSNMLETESQDLCCYCECC 307
           IH+RQEPSDSNGLC SSTNLWQQWHYDYGIFTVLTTPMFL PSN    E+QDLCCY ECC
Sbjct: 241 IHSRQEPSDSNGLCQSSTNLWQQWHYDYGIFTVLTTPMFLSPSNTHGNEAQDLCCYSECC 300

Query: 308 SPSGHLYLQIFDPCKNDIFMVNAPPESFIIQVGESADIISRGKLRSTLHSVCRPSKQEDL 367
           SPSGHLYLQIFDPCKNDIFMVNAPPESFIIQVGESADIISRGKLRSTLHSVCRPSK EDL
Sbjct: 301 SPSGHLYLQIFDPCKNDIFMVNAPPESFIIQVGESADIISRGKLRSTLHSVCRPSKHEDL 360

Query: 368 CREMFVVFLQPAWNKTFSMSDYPIESSMLSEDRKDLVDREGTAITREIQKIVPPLASRLK 427
           CREMFVVFLQPAWNKTFSMSDY IESS LSED++DLV++E T IT+EIQKIVPPLASRLK
Sbjct: 361 CREMFVVFLQPAWNKTFSMSDYSIESSTLSEDKRDLVEQEATVITQEIQKIVPPLASRLK 420

Query: 428 EGMTFAEFSRETTKQYYGGNGLQS 452
           EGMTFAEFSRETTKQYYGGNG ++
Sbjct: 421 EGMTFAEFSRETTKQYYGGNGTET 444

BLAST of Spg014463 vs. NCBI nr
Match: XP_023004799.1 (uncharacterized protein LOC111497992 isoform X1 [Cucurbita maxima])

HSP 1 Score: 781.2 bits (2016), Expect = 4.9e-222
Identity = 391/446 (87.67%), Postives = 417/446 (93.50%), Query Frame = 0

Query: 8   MEQNPKILEIYQLQYSDLLLLSSPNNSSSSPQENERMESVTKAILEALGPNGPGLLAITG 67
           ME+NP  LEIY+L+YS+L LLS+  +SSSS Q+NER+ES+TK+I EALGP+GPGLLAITG
Sbjct: 1   MEENPNKLEIYELRYSELFLLSTHCHSSSSLQDNERIESITKSIFEALGPSGPGLLAITG 60

Query: 68  VPNSSPLRRALLPLARNLALLNPDHRKRILKDHNLGSDVPLRNPERSVSSFAMQLKYTES 127
           VPNSS LRR LLPLAR LALLNPD RKRILKDH LGSDVPLRNPERSVSSFAMQLK+TES
Sbjct: 61  VPNSSVLRRTLLPLARKLALLNPDDRKRILKDHYLGSDVPLRNPERSVSSFAMQLKFTES 120

Query: 128 KQFMQNSQSQREDKHSPGSEIDHFCDSIKKELQENEFKHLGDAFKELGSCMMELGLHIAR 187
           K+FMQNSQSQR DK SPGSEIDHFCDSI+KE+Q+NEFKHLGD+FKELGSCM+ELGL IAR
Sbjct: 121 KEFMQNSQSQRGDKQSPGSEIDHFCDSIEKEVQDNEFKHLGDSFKELGSCMLELGLRIAR 180

Query: 188 ICDREIGGQELEQSLLESCTAKGRLIHYHSALDAQLLRKSTNCKGTARNQANSRRNKEQS 247
           ICD +IGGQELEQSLLESCTAKGRLIHYHSALDAQLLRKSTN KGTARNQANSRRNKEQS
Sbjct: 181 ICDAQIGGQELEQSLLESCTAKGRLIHYHSALDAQLLRKSTNFKGTARNQANSRRNKEQS 240

Query: 248 IHNRQEPSDSNGLCHSSTNLWQQWHYDYGIFTVLTTPMFLLPSNMLETESQDLCCYCECC 307
           IH+RQEPSDSNGLC SSTNLWQQWHYDYGIFTVLTTPMFL PSN    E+QDLCCY ECC
Sbjct: 241 IHSRQEPSDSNGLCQSSTNLWQQWHYDYGIFTVLTTPMFLSPSNTHGNEAQDLCCYRECC 300

Query: 308 SPSGHLYLQIFDPCKNDIFMVNAPPESFIIQVGESADIISRGKLRSTLHSVCRPSKQEDL 367
           SPSGHLYLQIFDPCKNDIFMVNAPPESFIIQVGESADIISRGKLRSTLHSVCRPSK EDL
Sbjct: 301 SPSGHLYLQIFDPCKNDIFMVNAPPESFIIQVGESADIISRGKLRSTLHSVCRPSKHEDL 360

Query: 368 CREMFVVFLQPAWNKTFSMSDYPIESSMLSEDRKDLVDREGTAITREIQKIVPPLASRLK 427
           CREMFVVFLQPAWNKTFSMSDY IESS LS+D++DLV++E T ITREIQKIVPPLASRLK
Sbjct: 361 CREMFVVFLQPAWNKTFSMSDYSIESSTLSDDKRDLVEQEATVITREIQKIVPPLASRLK 420

Query: 428 EGMTFAEFSRETTKQYYGGNGLQSNR 454
           EGMTFAEFSRETTKQYYGGNGLQSNR
Sbjct: 421 EGMTFAEFSRETTKQYYGGNGLQSNR 446

BLAST of Spg014463 vs. NCBI nr
Match: XP_023513669.1 (uncharacterized protein LOC111778210 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 777.3 bits (2006), Expect = 7.1e-221
Identity = 386/446 (86.55%), Postives = 415/446 (93.05%), Query Frame = 0

Query: 8   MEQNPKILEIYQLQYSDLLLLSSPNNSSSSPQENERMESVTKAILEALGPNGPGLLAITG 67
           ME+NP  LEIY+L+Y DL LLS+  +SSSS Q+NER+ES+TK+I EALGP+GPGLLAITG
Sbjct: 1   MEENPNKLEIYELRYLDLFLLSTHCHSSSSLQDNERIESITKSIFEALGPSGPGLLAITG 60

Query: 68  VPNSSPLRRALLPLARNLALLNPDHRKRILKDHNLGSDVPLRNPERSVSSFAMQLKYTES 127
           VPNSS LRR LLPLAR LALLNPD RKRILKDHNLGSDVPLRNPERSVSSFAMQLKYTES
Sbjct: 61  VPNSSVLRRTLLPLARKLALLNPDDRKRILKDHNLGSDVPLRNPERSVSSFAMQLKYTES 120

Query: 128 KQFMQNSQSQREDKHSPGSEIDHFCDSIKKELQENEFKHLGDAFKELGSCMMELGLHIAR 187
           K+FMQN+QSQR DK SPGSEIDHFCDSI+KE+ +NEFKHLGD+FKELGSCM+ELGL IAR
Sbjct: 121 KEFMQNNQSQRGDKQSPGSEIDHFCDSIEKEVHDNEFKHLGDSFKELGSCMLELGLRIAR 180

Query: 188 ICDREIGGQELEQSLLESCTAKGRLIHYHSALDAQLLRKSTNCKGTARNQANSRRNKEQS 247
           +CD +IGGQELEQSLLESC AKGRLIHYHSALDAQLLRKST+CKGTARNQANSRRNKEQS
Sbjct: 181 VCDAQIGGQELEQSLLESCIAKGRLIHYHSALDAQLLRKSTHCKGTARNQANSRRNKEQS 240

Query: 248 IHNRQEPSDSNGLCHSSTNLWQQWHYDYGIFTVLTTPMFLLPSNMLETESQDLCCYCECC 307
           IH++QEPSDSNGLC S TNLWQQWHYDYGIFTVLTTPMFL PSN    E+QDLCCY ECC
Sbjct: 241 IHSKQEPSDSNGLCQSCTNLWQQWHYDYGIFTVLTTPMFLSPSNTHGNEAQDLCCYSECC 300

Query: 308 SPSGHLYLQIFDPCKNDIFMVNAPPESFIIQVGESADIISRGKLRSTLHSVCRPSKQEDL 367
           SPSGHLYLQIFDPCKNDIFMVNAPPESFIIQVGESADIISRGKLRSTLHSVCRPSK EDL
Sbjct: 301 SPSGHLYLQIFDPCKNDIFMVNAPPESFIIQVGESADIISRGKLRSTLHSVCRPSKHEDL 360

Query: 368 CREMFVVFLQPAWNKTFSMSDYPIESSMLSEDRKDLVDREGTAITREIQKIVPPLASRLK 427
           CRE+FVVFLQPAWNKTFSMSDY IESSM S+D++DLV++E T ITREIQKIVPPLASRLK
Sbjct: 361 CREIFVVFLQPAWNKTFSMSDYSIESSMSSKDKRDLVEQEATVITREIQKIVPPLASRLK 420

Query: 428 EGMTFAEFSRETTKQYYGGNGLQSNR 454
           EGMTFAEFSRETTKQYYGGNGLQSNR
Sbjct: 421 EGMTFAEFSRETTKQYYGGNGLQSNR 446

BLAST of Spg014463 vs. ExPASy TrEMBL
Match: A0A6J1H636 (uncharacterized protein LOC111460876 OS=Cucurbita moschata OX=3662 GN=LOC111460876 PE=4 SV=1)

HSP 1 Score: 790.4 bits (2040), Expect = 3.9e-225
Identity = 394/446 (88.34%), Postives = 418/446 (93.72%), Query Frame = 0

Query: 8   MEQNPKILEIYQLQYSDLLLLSSPNNSSSSPQENERMESVTKAILEALGPNGPGLLAITG 67
           ME+NP  LEIY+L+YSDL LLS+  +SSSS Q NER+ES+TK+I EALGP+GPGLLAITG
Sbjct: 1   MEENPNKLEIYELRYSDLFLLSTHCHSSSSLQGNERIESITKSIFEALGPSGPGLLAITG 60

Query: 68  VPNSSPLRRALLPLARNLALLNPDHRKRILKDHNLGSDVPLRNPERSVSSFAMQLKYTES 127
           VPNSS LRR LLPLAR LALLNPD RKRILKDHNLGSDVPLRNPERSVSSFAMQLKYTES
Sbjct: 61  VPNSSVLRRTLLPLARKLALLNPDDRKRILKDHNLGSDVPLRNPERSVSSFAMQLKYTES 120

Query: 128 KQFMQNSQSQREDKHSPGSEIDHFCDSIKKELQENEFKHLGDAFKELGSCMMELGLHIAR 187
           K+FMQN+QSQR DK SPGSEIDHFCDSI+KE+Q+NEFKHLGD+FKELGSCM+ELGLHIAR
Sbjct: 121 KEFMQNNQSQRGDKQSPGSEIDHFCDSIEKEVQDNEFKHLGDSFKELGSCMLELGLHIAR 180

Query: 188 ICDREIGGQELEQSLLESCTAKGRLIHYHSALDAQLLRKSTNCKGTARNQANSRRNKEQS 247
           ICD +IGGQELEQSLLESCTAKGRLIHYHSALDAQLLRKSTNCKGTARNQANSRRNKEQS
Sbjct: 181 ICDAQIGGQELEQSLLESCTAKGRLIHYHSALDAQLLRKSTNCKGTARNQANSRRNKEQS 240

Query: 248 IHNRQEPSDSNGLCHSSTNLWQQWHYDYGIFTVLTTPMFLLPSNMLETESQDLCCYCECC 307
           IH+RQEPSDSNGLC S TNLWQQWHYDYGIFTVLTTPMFL PSN    E+QDLCCY ECC
Sbjct: 241 IHSRQEPSDSNGLCQSITNLWQQWHYDYGIFTVLTTPMFLSPSNTHGNEAQDLCCYSECC 300

Query: 308 SPSGHLYLQIFDPCKNDIFMVNAPPESFIIQVGESADIISRGKLRSTLHSVCRPSKQEDL 367
           SPSGHLYLQIFDPCKNDIFMVNAPPESFIIQVGES+DIISRGKLRSTLHSVCRPSK EDL
Sbjct: 301 SPSGHLYLQIFDPCKNDIFMVNAPPESFIIQVGESSDIISRGKLRSTLHSVCRPSKHEDL 360

Query: 368 CREMFVVFLQPAWNKTFSMSDYPIESSMLSEDRKDLVDREGTAITREIQKIVPPLASRLK 427
           CREMFVVFLQPAWNKTFSMSDY IESS LSED++DLV++E T ITREIQKIVPPLASRLK
Sbjct: 361 CREMFVVFLQPAWNKTFSMSDYSIESSTLSEDKRDLVEQEATVITREIQKIVPPLASRLK 420

Query: 428 EGMTFAEFSRETTKQYYGGNGLQSNR 454
           EGMTFAEFSRETTKQYYGGNGLQSNR
Sbjct: 421 EGMTFAEFSRETTKQYYGGNGLQSNR 446

BLAST of Spg014463 vs. ExPASy TrEMBL
Match: A0A6J1KXC5 (uncharacterized protein LOC111497992 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111497992 PE=4 SV=1)

HSP 1 Score: 781.2 bits (2016), Expect = 2.4e-222
Identity = 391/446 (87.67%), Postives = 417/446 (93.50%), Query Frame = 0

Query: 8   MEQNPKILEIYQLQYSDLLLLSSPNNSSSSPQENERMESVTKAILEALGPNGPGLLAITG 67
           ME+NP  LEIY+L+YS+L LLS+  +SSSS Q+NER+ES+TK+I EALGP+GPGLLAITG
Sbjct: 1   MEENPNKLEIYELRYSELFLLSTHCHSSSSLQDNERIESITKSIFEALGPSGPGLLAITG 60

Query: 68  VPNSSPLRRALLPLARNLALLNPDHRKRILKDHNLGSDVPLRNPERSVSSFAMQLKYTES 127
           VPNSS LRR LLPLAR LALLNPD RKRILKDH LGSDVPLRNPERSVSSFAMQLK+TES
Sbjct: 61  VPNSSVLRRTLLPLARKLALLNPDDRKRILKDHYLGSDVPLRNPERSVSSFAMQLKFTES 120

Query: 128 KQFMQNSQSQREDKHSPGSEIDHFCDSIKKELQENEFKHLGDAFKELGSCMMELGLHIAR 187
           K+FMQNSQSQR DK SPGSEIDHFCDSI+KE+Q+NEFKHLGD+FKELGSCM+ELGL IAR
Sbjct: 121 KEFMQNSQSQRGDKQSPGSEIDHFCDSIEKEVQDNEFKHLGDSFKELGSCMLELGLRIAR 180

Query: 188 ICDREIGGQELEQSLLESCTAKGRLIHYHSALDAQLLRKSTNCKGTARNQANSRRNKEQS 247
           ICD +IGGQELEQSLLESCTAKGRLIHYHSALDAQLLRKSTN KGTARNQANSRRNKEQS
Sbjct: 181 ICDAQIGGQELEQSLLESCTAKGRLIHYHSALDAQLLRKSTNFKGTARNQANSRRNKEQS 240

Query: 248 IHNRQEPSDSNGLCHSSTNLWQQWHYDYGIFTVLTTPMFLLPSNMLETESQDLCCYCECC 307
           IH+RQEPSDSNGLC SSTNLWQQWHYDYGIFTVLTTPMFL PSN    E+QDLCCY ECC
Sbjct: 241 IHSRQEPSDSNGLCQSSTNLWQQWHYDYGIFTVLTTPMFLSPSNTHGNEAQDLCCYRECC 300

Query: 308 SPSGHLYLQIFDPCKNDIFMVNAPPESFIIQVGESADIISRGKLRSTLHSVCRPSKQEDL 367
           SPSGHLYLQIFDPCKNDIFMVNAPPESFIIQVGESADIISRGKLRSTLHSVCRPSK EDL
Sbjct: 301 SPSGHLYLQIFDPCKNDIFMVNAPPESFIIQVGESADIISRGKLRSTLHSVCRPSKHEDL 360

Query: 368 CREMFVVFLQPAWNKTFSMSDYPIESSMLSEDRKDLVDREGTAITREIQKIVPPLASRLK 427
           CREMFVVFLQPAWNKTFSMSDY IESS LS+D++DLV++E T ITREIQKIVPPLASRLK
Sbjct: 361 CREMFVVFLQPAWNKTFSMSDYSIESSTLSDDKRDLVEQEATVITREIQKIVPPLASRLK 420

Query: 428 EGMTFAEFSRETTKQYYGGNGLQSNR 454
           EGMTFAEFSRETTKQYYGGNGLQSNR
Sbjct: 421 EGMTFAEFSRETTKQYYGGNGLQSNR 446

BLAST of Spg014463 vs. ExPASy TrEMBL
Match: A0A0A0K8T7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G387710 PE=4 SV=1)

HSP 1 Score: 739.6 bits (1908), Expect = 7.9e-210
Identity = 375/446 (84.08%), Postives = 406/446 (91.03%), Query Frame = 0

Query: 8   MEQNPKILEIYQLQYSDLLLLSSPNNSSSSPQENERMESVTKAILEALGPNGPGLLAITG 67
           M  NPK+LEIY+L YSDLLLLS+  +SSSS QEN+R+ES+TK+ILEALGPNGPGLLAITG
Sbjct: 1   MGGNPKVLEIYELPYSDLLLLSAAYHSSSSLQENQRIESITKSILEALGPNGPGLLAITG 60

Query: 68  VPNSSPLRRALLPLARNLALLNPDHRKRILKDHNLGSDVPLRNPERSVSSFAMQLKYTES 127
           VPNSS LRRALLPLAR LALLNPDHRK+ILKDHNLGSDVPLRNPERSVSSFAMQLKYTES
Sbjct: 61  VPNSSVLRRALLPLARKLALLNPDHRKQILKDHNLGSDVPLRNPERSVSSFAMQLKYTES 120

Query: 128 KQFMQNSQSQREDKHSPGSEIDHFCDSIKKELQENEFKHLGDAFKELGSCMMELGLHIAR 187
           K+FMQN+QSQ EDK S GSE+D FC SI+ +L++NEF+HLG++FKELGSCMMELGL IAR
Sbjct: 121 KEFMQNNQSQIEDKQSSGSELDSFCHSIENKLKDNEFEHLGNSFKELGSCMMELGLRIAR 180

Query: 188 ICDREIGGQELEQSLLESCTAKGRLIHYHSALDAQLLRKSTNCKGTARNQANSRRNKEQS 247
           ICDREIGG+ELE+SLLESCTAKGRLIHYHSALDAQLLRK  N KGTARNQA+SRRN+EQS
Sbjct: 181 ICDREIGGRELEESLLESCTAKGRLIHYHSALDAQLLRKPANSKGTARNQASSRRNREQS 240

Query: 248 IHNRQEPSDSNGLCHSSTNLWQQWHYDYGIFTVLTTPMFLLPSNMLETESQDLCCYCECC 307
           I +R +PSD  GLC SSTNLWQQWHYDYGIFTVLTTPMFL PSN LE+  QDL C  E  
Sbjct: 241 IQSRHDPSDRKGLCQSSTNLWQQWHYDYGIFTVLTTPMFLSPSNTLESGLQDLWCCSERT 300

Query: 308 SPSGHLYLQIFDPCKNDIFMVNAPPESFIIQVGESADIISRGKLRSTLHSVCRPSKQEDL 367
           SPSGHLYLQIFDPCKND+FMVN+PPESFIIQVGESADIISRGKLRSTLHSV RPSKQEDL
Sbjct: 301 SPSGHLYLQIFDPCKNDVFMVNSPPESFIIQVGESADIISRGKLRSTLHSVSRPSKQEDL 360

Query: 368 CREMFVVFLQPAWNKTFSMSDYPIESSMLSEDRKDLVDREGTAITREIQKIVPPLASRLK 427
           CREMFVVFLQPAWNKTFSMS +  ESSML EDRKDLV+ EGT ITREIQKIVPPL SRLK
Sbjct: 361 CREMFVVFLQPAWNKTFSMSGHLTESSMLPEDRKDLVEEEGTLITREIQKIVPPLVSRLK 420

Query: 428 EGMTFAEFSRETTKQYYGGNGLQSNR 454
           EGMTFAEFSRETTKQYYGG+GLQSNR
Sbjct: 421 EGMTFAEFSRETTKQYYGGSGLQSNR 446

BLAST of Spg014463 vs. ExPASy TrEMBL
Match: A0A6J1KVL4 (uncharacterized protein LOC111497992 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111497992 PE=4 SV=1)

HSP 1 Score: 728.0 bits (1878), Expect = 2.4e-206
Identity = 362/400 (90.50%), Postives = 378/400 (94.50%), Query Frame = 0

Query: 54  ALGPNGPGLLAITGVPNSSPLRRALLPLARNLALLNPDHRKRILKDHNLGSDVPLRNPER 113
           ALGP+GPGLLAITGVPNSS LRR LLPLAR LALLNPD RKRILKDH LGSDVPLRNPER
Sbjct: 4   ALGPSGPGLLAITGVPNSSVLRRTLLPLARKLALLNPDDRKRILKDHYLGSDVPLRNPER 63

Query: 114 SVSSFAMQLKYTESKQFMQNSQSQREDKHSPGSEIDHFCDSIKKELQENEFKHLGDAFKE 173
           SVSSFAMQLK+TESK+FMQNSQSQR DK SPGSEIDHFCDSI+KE+Q+NEFKHLGD+FKE
Sbjct: 64  SVSSFAMQLKFTESKEFMQNSQSQRGDKQSPGSEIDHFCDSIEKEVQDNEFKHLGDSFKE 123

Query: 174 LGSCMMELGLHIARICDREIGGQELEQSLLESCTAKGRLIHYHSALDAQLLRKSTNCKGT 233
           LGSCM+ELGL IARICD +IGGQELEQSLLESCTAKGRLIHYHSALDAQLLRKSTN KGT
Sbjct: 124 LGSCMLELGLRIARICDAQIGGQELEQSLLESCTAKGRLIHYHSALDAQLLRKSTNFKGT 183

Query: 234 ARNQANSRRNKEQSIHNRQEPSDSNGLCHSSTNLWQQWHYDYGIFTVLTTPMFLLPSNML 293
           ARNQANSRRNKEQSIH+RQEPSDSNGLC SSTNLWQQWHYDYGIFTVLTTPMFL PSN  
Sbjct: 184 ARNQANSRRNKEQSIHSRQEPSDSNGLCQSSTNLWQQWHYDYGIFTVLTTPMFLSPSNTH 243

Query: 294 ETESQDLCCYCECCSPSGHLYLQIFDPCKNDIFMVNAPPESFIIQVGESADIISRGKLRS 353
             E+QDLCCY ECCSPSGHLYLQIFDPCKNDIFMVNAPPESFIIQVGESADIISRGKLRS
Sbjct: 244 GNEAQDLCCYRECCSPSGHLYLQIFDPCKNDIFMVNAPPESFIIQVGESADIISRGKLRS 303

Query: 354 TLHSVCRPSKQEDLCREMFVVFLQPAWNKTFSMSDYPIESSMLSEDRKDLVDREGTAITR 413
           TLHSVCRPSK EDLCREMFVVFLQPAWNKTFSMSDY IESS LS+D++DLV++E T ITR
Sbjct: 304 TLHSVCRPSKHEDLCREMFVVFLQPAWNKTFSMSDYSIESSTLSDDKRDLVEQEATVITR 363

Query: 414 EIQKIVPPLASRLKEGMTFAEFSRETTKQYYGGNGLQSNR 454
           EIQKIVPPLASRLKEGMTFAEFSRETTKQYYGGNGLQSNR
Sbjct: 364 EIQKIVPPLASRLKEGMTFAEFSRETTKQYYGGNGLQSNR 403

BLAST of Spg014463 vs. ExPASy TrEMBL
Match: A0A5A7V7E3 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold111G001110 PE=4 SV=1)

HSP 1 Score: 724.5 bits (1869), Expect = 2.6e-205
Identity = 371/446 (83.18%), Postives = 401/446 (89.91%), Query Frame = 0

Query: 8   MEQNPKILEIYQLQYSDLLLLSSPNNSSSSPQENERMESVTKAILEALGPNGPGLLAITG 67
           M +NPKIL+I++L YSDLLLLS+P +S SS QE+ERMES+TK+ILEALGPNGPGLLAI G
Sbjct: 1   MGENPKILDIHELSYSDLLLLSTPYHSPSSLQESERMESITKSILEALGPNGPGLLAIIG 60

Query: 68  VPNSSPLRRALLPLARNLALLNPDHRKRILKDHNLGSDVPLRNPERSVSSFAMQLKYTES 127
           VPNSS LRRALLPLAR LALLNPDHRKRILKDHNLGSDVPLRNPERSVSSFAMQLKYTES
Sbjct: 61  VPNSSVLRRALLPLARKLALLNPDHRKRILKDHNLGSDVPLRNPERSVSSFAMQLKYTES 120

Query: 128 KQFMQNSQSQREDKHSPGSEIDHFCDSIKKELQENEFKHLGDAFKELGSCMMELGLHIAR 187
           K+FMQN+QSQ EDK S  SE D FC SI+ ++ ++EFKHLG++FKELGSCMMELGL IAR
Sbjct: 121 KEFMQNNQSQIEDKQSSVSEADSFCHSIENKVIDDEFKHLGNSFKELGSCMMELGLRIAR 180

Query: 188 ICDREIGGQELEQSLLESCTAKGRLIHYHSALDAQLLRKSTNCKGTARNQANSRRNKEQS 247
           ICDREIGGQELE+SLLESCTAKGRLIHYHSALDAQLL K  N KGTARNQA+SRRN+EQS
Sbjct: 181 ICDREIGGQELEESLLESCTAKGRLIHYHSALDAQLLSKPANSKGTARNQASSRRNREQS 240

Query: 248 IHNRQEPSDSNGLCHSSTNLWQQWHYDYGIFTVLTTPMFLLPSNMLETESQDLCCYCECC 307
           I +R + S+  GL  SSTNLWQQWHYDYGIFTVLTTPMFL PSN LET  QDL C  E  
Sbjct: 241 IQSRHDTSNRKGLSQSSTNLWQQWHYDYGIFTVLTTPMFLSPSNTLETGLQDLWCCSERT 300

Query: 308 SPSGHLYLQIFDPCKNDIFMVNAPPESFIIQVGESADIISRGKLRSTLHSVCRPSKQEDL 367
           SPSGHLYLQIFDPCKND+FMVN+PPESFIIQVGESADIISRGKLRSTLHSV RPSKQEDL
Sbjct: 301 SPSGHLYLQIFDPCKNDVFMVNSPPESFIIQVGESADIISRGKLRSTLHSVSRPSKQEDL 360

Query: 368 CREMFVVFLQPAWNKTFSMSDYPIESSMLSEDRKDLVDREGTAITREIQKIVPPLASRLK 427
           CREMFVVFLQPAWNKTFSMS +  ESSML EDRKDLV+ EGT ITREIQKIVPPLASRLK
Sbjct: 361 CREMFVVFLQPAWNKTFSMSGHLTESSMLPEDRKDLVEEEGTLITREIQKIVPPLASRLK 420

Query: 428 EGMTFAEFSRETTKQYYGGNGLQSNR 454
           EGMTFA+FSRETTKQYYGG+GLQSNR
Sbjct: 421 EGMTFAQFSRETTKQYYGGSGLQSNR 446

BLAST of Spg014463 vs. TAIR 10
Match: AT3G63290.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 413.7 bits (1062), Expect = 1.9e-115
Identity = 237/441 (53.74%), Postives = 296/441 (67.12%), Query Frame = 0

Query: 13  KILEIYQLQYSDLLLLSSPNNSSSSPQENERMESVTKAILEALGPNGPGLLAITGVPNSS 72
           +ILE YQL +SDLLL S             R + ++K +++ALGP GPGLL ITGV  S+
Sbjct: 5   EILEPYQLSFSDLLLSS-----------RNRSQWISKNVMDALGPTGPGLLCITGVLGSA 64

Query: 73  PLRRALLPLARNLALLNPDHRKRILKDHNLGSDVPLRNPERSVSSFAMQLKYTESKQFMQ 132
            LRR LLP+AR LALL+PD RK IL +H+LGSDVPL+NPER VSSFAMQL Y   ++   
Sbjct: 65  FLRRKLLPMARKLALLDPDKRKLILMEHHLGSDVPLKNPERDVSSFAMQLNY---ERTTY 124

Query: 133 NSQSQREDKHSPGSEIDHFCDSIKKELQENEFKHLGDAFKELGSCMMELGLHIARICDRE 192
            S   +      GS++D       +E  ++ F +LG AFKELG CM ELGL IAR+CDRE
Sbjct: 125 KSSLGKLWFDEAGSKLD------LQEDDDDAFTNLGGAFKELGFCMRELGLSIARLCDRE 184

Query: 193 IGGQELEQSLLESCTAKGRLIHYHSALDAQLLRKSTNCKGTARNQANSRRNKEQSIHNRQ 252
           IGG  LE+SLL+SCTAKGRLIHYHSA D   LR+S   +  + N+ +S+R  + +     
Sbjct: 185 IGGGLLEESLLDSCTAKGRLIHYHSAADKYALRESQR-RNQSGNRVSSKRRVQNAAEQEL 244

Query: 253 EPSDSNGLCHSSTNLWQQWHYDYGIFTVLTTPMFLLPSNMLETESQDLCCYCECCSPSGH 312
              +  GL  S  NLWQQWHYDYGIFTVLT PMFL P +           Y E    S H
Sbjct: 245 NRRNGAGLSGSHFNLWQQWHYDYGIFTVLTDPMFLSPYS-----------YQEFSLMSSH 304

Query: 313 LYLQIFDPCKNDIFMVNAPPESFIIQVGESADIISRGKLRSTLHSVCRPSKQEDLCREMF 372
            YLQI+ P KN  +MV  P +SF++Q+GESADI+S+GKLRSTLH VC+P K + + RE F
Sbjct: 305 SYLQIYHPSKNKFYMVKTPQDSFLVQIGESADILSKGKLRSTLHCVCKPEKLDHVSRETF 364

Query: 373 VVFLQPAWNKTFSMSDYPIESSMLSEDRKDLVDREGTAITREIQKIVPPLASRLKEGMTF 432
           VVFL P W++TFS+S+Y +E   L  D  ++V R       ++Q IVPPL+SRL++GMTF
Sbjct: 365 VVFLHPKWSQTFSVSEYTMEH--LRSD--EVVPRP------DLQNIVPPLSSRLRDGMTF 403

Query: 433 AEFSRETTKQYYGGNGLQSNR 454
           AEFSRETTKQYYGGNGLQSNR
Sbjct: 425 AEFSRETTKQYYGGNGLQSNR 403

BLAST of Spg014463 vs. TAIR 10
Match: AT3G63290.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 311.6 bits (797), Expect = 1.0e-84
Identity = 169/301 (56.15%), Postives = 209/301 (69.44%), Query Frame = 0

Query: 156 KKELQENE---FKHLGDAFKELGSCMMELGLHIARICDREIGGQELEQSLLESCTAKGRL 215
           K +LQE++   F +LG AFKELG CM ELGL IAR+CDREIGG  LE+SLL+SCTAKGRL
Sbjct: 25  KLDLQEDDDDAFTNLGGAFKELGFCMRELGLSIARLCDREIGGGLLEESLLDSCTAKGRL 84

Query: 216 IHYHSALDAQLLRKSTNCKGTARNQANSRRNKEQSIHNRQEPSDSNGLCHSSTNLWQQWH 275
           IHYHSA D   LR+S   +  + N+ +S+R  + +        +  GL  S  NLWQQWH
Sbjct: 85  IHYHSAADKYALRESQR-RNQSGNRVSSKRRVQNAAEQELNRRNGAGLSGSHFNLWQQWH 144

Query: 276 YDYGIFTVLTTPMFLLPSNMLETESQDLCCYCECCSPSGHLYLQIFDPCKNDIFMVNAPP 335
           YDYGIFTVLT PMFL P +           Y E    S H YLQI+ P KN  +MV  P 
Sbjct: 145 YDYGIFTVLTDPMFLSPYS-----------YQEFSLMSSHSYLQIYHPSKNKFYMVKTPQ 204

Query: 336 ESFIIQVGESADIISRGKLRSTLHSVCRPSKQEDLCREMFVVFLQPAWNKTFSMSDYPIE 395
           +SF++Q+GESADI+S+GKLRSTLH VC+P K + + RE FVVFL P W++TFS+S+Y +E
Sbjct: 205 DSFLVQIGESADILSKGKLRSTLHCVCKPEKLDHVSRETFVVFLHPKWSQTFSVSEYTME 264

Query: 396 SSMLSEDRKDLVDREGTAITREIQKIVPPLASRLKEGMTFAEFSRETTKQYYGGNGLQSN 454
              L  D  ++V R       ++Q IVPPL+SRL++GMTFAEFSRETTKQYYGGNGLQSN
Sbjct: 265 H--LRSD--EVVPRP------DLQNIVPPLSSRLRDGMTFAEFSRETTKQYYGGNGLQSN 303

BLAST of Spg014463 vs. TAIR 10
Match: AT4G13400.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 91.3 bits (225), Expect = 2.2e-18
Identity = 106/368 (28.80%), Postives = 148/368 (40.22%), Query Frame = 0

Query: 31  PNNSSSSPQENERMES---VTKAILEALGPNGPGLLAITGVPNSSPLRRALLPLARNLAL 90
           P  S+ +   +E  ES   ++  I E  GPNG G+L++  VP  S LR+ LL LA  LA 
Sbjct: 10  PTVSTVTISYSELKESNIDLSARIEEGFGPNGLGILSVKDVPGYSALRQNLLQLAPRLAG 69

Query: 91  LNPDHRKRILKD----HNLGSDVPLRNPERSVSSFAMQLKYTESKQFMQNSQSQREDKHS 150
           L P+  KR L+D    +N G        E           Y    Q +  S S    ++ 
Sbjct: 70  L-PEEVKRELEDPHSRYNFGWSHGKEKLESGKLDMLKGSYYANPLQDVPTSNSYEIQRY- 129

Query: 151 PGSEIDHFCDSIKKELQENEFKHLGDAFKELGSCMMELGLHIARICDREIG-------GQ 210
                  +C S       N    L  AFK LG  M E+GL +A  CD+ +         Q
Sbjct: 130 -----PSYCGS--NIWPRNSLPELEGAFKALGKLMFEVGLMVAYHCDQYVSKGIKQHEKQ 189

Query: 211 ELEQSLLESCTAKGRLIHYHSALDAQLLRKSTNCKGTARNQANSRRNKEQSIHNRQEPSD 270
            LE+ LL S   KGRL++Y  A                         +E S H+      
Sbjct: 190 NLEKILLGSRCHKGRLLYYFPA-------------------------QESSTHD------ 249

Query: 271 SNGLCHSSTNLWQQWHYDYGIFTVLTTPMFLLPSNMLETESQDLCCYCECCSPSGHLYLQ 330
                + S + W  WH D+G  T LT  +F          S+D      C  P+  LY+Q
Sbjct: 250 -----NDSISSWCGWHTDHGSLTGLTRAIF----------SRD-SVEVPCPDPASGLYIQ 309

Query: 331 IFDPCKNDIFMVNAPPESFIIQVGESADIISRGKLRSTLHSVCRPSKQE--DLCREMFVV 383
                   I  V    +    Q+GE+  I+S G L +T H V  P  +E   L R  F +
Sbjct: 310 ---TRSGQIVKVVYGEDEIAYQIGETTSILSSGYLCATPHCVRAPQGEEARGLERSTFAL 318

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022959987.18.1e-22588.34uncharacterized protein LOC111460876 [Cucurbita moschata][more]
KAG6593098.11.8e-22488.34hypothetical protein SDJN03_12574, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG7025506.13.8e-22287.61hypothetical protein SDJN02_12001 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023004799.14.9e-22287.67uncharacterized protein LOC111497992 isoform X1 [Cucurbita maxima][more]
XP_023513669.17.1e-22186.55uncharacterized protein LOC111778210 isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1H6363.9e-22588.34uncharacterized protein LOC111460876 OS=Cucurbita moschata OX=3662 GN=LOC1114608... [more]
A0A6J1KXC52.4e-22287.67uncharacterized protein LOC111497992 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A0A0K8T77.9e-21084.08Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G387710 PE=4 SV=1[more]
A0A6J1KVL42.4e-20690.50uncharacterized protein LOC111497992 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A5A7V7E32.6e-20583.18Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
Match NameE-valueIdentityDescription
AT3G63290.11.9e-11553.742-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G63290.21.0e-8456.152-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G13400.12.2e-1828.802-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 316..369
e-value: 1.0E-4
score: 22.7
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 33..411
e-value: 2.8E-27
score: 97.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 234..259
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 12..430
NoneNo IPR availablePANTHERPTHR10209:SF5532-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEINcoord: 12..430
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 42..447

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg014463.1Spg014463.1mRNA