Spg014393 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg014393
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionpalmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like
Locationscaffold3: 42459288 .. 42464459 (+)
RNA-Seq ExpressionSpg014393
SyntenySpg014393
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGTCTCAACTCAACTACACAATCCCTACAAATCTATTGGAATCTTCAAAGGAGCCAAAGGAGAAGAAGCCTTCAATGAAGTGGACTAAGTTAGATAATAATCATGTTGTTCCTACTTCTTATCTTCTCCTTATCCACATACTTTGCATTTTCGCACCATTCTATTTCAATTGGAATGCTTTTTGGGTTGCTTTAGCATTGGCTGTGGTTACCGGTATGACCATTACGGTCTCGTATCACAGAAACCTTTCACACAAAAGCTTCACACTCCCTAAATGGCTTGAGTATTTGCTTGCTTATTGTGCCGTTCATGCATTTCAGGTTTGATATTTCGATCCTCATTCTTAAATTAAGATGGAAGAAAAACAGAGATTATTAGTGGTTTAAATACTATTTCGGTTCCTATACTTTTGGTTTTGGTTCATTTTGGTCTTTAACTTTCAAAATGTTCATTTTAGTCCGTACTTTCAAAATGTTCATTTTGGTTTATGTACTTTGGGATTTGGTTCGTTTTGGTCCATTCATTTTCAATTTGTTCTTATTTTTTGGATTCATAATTTTAATATGACACTTTAATTACTAAATTTATTAAAATGTAATGATAAATTTGAGTAAAGAGTTTCGATTGTGTATAAAAGTTACGCTTAAATCTTGTATAAAATTTAGCATGACTAAAAATGTAATGCTTTTTTAAAATATAGAGACTAAAATGAACTTTTTGAAAGTAAAGGGACCAAAATGAACTAAATTCCAAAGTACAGTGACTAAAATGAACCAAAGTCAAAAGTACCGTGACCAAAATAGTATTTAAATCATAAATAAAAAGTTTTTTTTTTAAATACCATAAATAAAAAGTTCCTCGCCATTTTACTAGGAATGACCGTAAAGATAGTTGTAAATCGTATCATTTAGATAAAATAATACAAAAATGAAATCTAAAATAAAATATTCTCAATTATAGCAAAATATACGGAGCATGTAAATTAGTAATTAAAAGTAATCAATATGTAGGTTAATTAAGAATTGCTACTCATTATAAATAATCAATATTTAGGTTAATCAACAATAATCACAATATCACATAGAAACCAACAAAATTGTAGTTCAACTGGTATAAAGTTCGTATCTTCGACTAAGAGGTTAGAGGATTCAAATCCTCCACCCCACATATTGATATATATATATATATATATAGTTCGTATCTTCGACCAAGAGGTTAGAGGATTCAAATCCTCCACCCCACATGTTGATATATATATATATATATATAATAATAAAGAAAAACATAGTGATTAATGTTATTAAGTTAATATTTTTTTTCACCATCAATTTTTATTTTCAGCCACCTAATCTAGGGTTTTTTTCCTTTTCAAATAGTCGAGGTTGATTAAAACTTAATTGAATTATTTTTAGTTTTTAGTCTTTGAAAATCATTCTAATTTTCTTTCCATTGTTTTTATTTTGTTAATAAAGTCTTTTAATTCTTAACCAAATTCCAAAAACAATAACATAATTTTAAAACTACTCCTTTTTAATTTTTAGAACTTGACTTAGTTTTTAAAAACATGGATCTTTTCTTTTTTTGTCCTCTTTCTTTTTTCTTTTCCTTTCTATTTGTTTTTTTAAAAAAATTAAATAACCTTTTTTTCTTTTATTTAAAAAAAATCAAAAAAGGTAGTTACATTTTTTTTATGTTTTAAACGGATTGAATATTATATTACAATTTATTCTCTTTTTTTAAGAAATTGAAATTTCATGAAGTATTAATTATTTTGATTTTTTTCTTTTAATTTGATGTTACTTGCAGTTTATATATATATATATATATATATATATATATATATATATATAATCAAAGAAATTAGAAAAAAAAATAATATTTAATTAAATTTCAATTTCTTTAAGAAAATAAAATAAATTGTAATACAATATTCAATGTGTTTAAAATATTAAACAAAAAAAATGTAAAAAAAAGTTAAAAAGGTAGGTTATTTCATTTTTAAACAAAATAACAAATAAAAAGGAAAAGGAAAAAAAGAAAAAAAAAACGAAGGACAAATAAAAGAGAGGGAAAAAAAAAAAAGAAAAAAAGAAACATTTATAGGTCGTCTTTTTTTTTTATATAAAAAAATCGTGGTAGGGTCGTCTCTTGCTATCAAATTTTTTTTTTTTTTTTTTTTTTTTTGTAAAAAATTGTGACATATGGCTTGCACATTGAAGAGACGACTTACCAGATAGATTTAAAACCACAATATTCTTCTAATACGTTTCAAAATAAATTATTTTTTTTCTTCCTTTTCAAAATGAATTTATTACCATAATAAGTTTTAAAAATTATAATATTAATTAAAAACAAGCTTGTTATTTAAGGAGTCTAAGGAGTTCAATTTCATTATTGGTTTTGCAAAACCAAACCAAACTACACTAGGAAGAATATCAACTTGCTAAAATTTAAAGGTGACAAGCTATATACTAGAGCATTCTCTTTTACGGGAAAAAACTATAGATCCTTCTAGACTTGCTTCACAATTCTTTCAATTTCTAAATATTCGTGTTTTGAAATAATTGGTTTATTTTATTTTACTTTTAGGGTGATCCAATCGGTTGGGTGAGCATCCATAGATATCATCATCAATATATAGATACTGAAAGAGATCCACACAGTCCTATTGGAGGATTTTGGTTTAGTTATATAACATGGCTATTTGATTTAAATACTTTGAATAAAAAAGTTCAATTCGACCATGTTAGTGATTTTCGAGAGACAAAAAAAGACACGGGAATATTCCAACATAAAAATCAGGATAATGTCAATGACTTGAAGAATCAACTCTTCTATAGGCTGATGAATGAAACTTACATTCTTCATCCAATTGCTCTAGCAGTCTTGTTGTATGCCATCGGAGGAGTTCCTTTTATCCTATGGGGATTGGTAAACAAAATCACTCCTTTTTTCTTTTCCTTTTCACCATTAAAATGTCATTATTTTTAGGGAAAAATACGTTTTTGGTCCCCGAGGTTTGGGTAAACCCTAAATCTTAACAGGATTTTGACGTGGCACTGTTATTTTAATTAAAAAAATTAATTTTTATTTTTTTAAATAAAAATATATTAAAATAATAAAAAAAATTATTATTTTATTATTTTTTTTTAATTATTTCTCCCCCTCTTTCTTCTCCTTCTTCCTCTTTCCCATCTCCTTCTTCAACTCTGGTGGCCGGCCGCCGCCGGCTGCCGACGAACGGTGGCGGGGGCCACCGGATTTTTTTTTTTCTTCTTTGTTCTTCTTCCCTTCCTCCCCTTTCTTTTCTTTCTTCTATTTTTTTTTTTTTTTTTTTAAACAGAGGGAGATTTGGGGAGGAGGAGCTCCTCCCCCTCTCGTTTCAGATCTGAACAAGAACAACCCTGATGAGCAGATCCTCAAATCACTCGGCAGAAACCAAAACCACAAGGAAAAAGAATCACAAAAATCCAAAGAAACCCATGAAAAAACAACCAAAACGAAGAGGGGGTGAAACAAACAAACAAATTCTCCAATACCCACCAAGGGAAAATCGCAGGGGAAGATGAAACAGCAATACCCAGAAGACAAAAACAATAAAAAAAGAAAAGAGAAGAACCGTCCGCAGCAGCACAAGAAACGAAATGGGGGCAAGAGAATACGTACAACACAAGGAAGAAATAGCGAGAAACGCAACAAGAATGAAGAGAGAGAGAAGGAGAGGGAAATACGAAAGAGAAACCTATCGTTCTTTATGTTTGGTGCAGAGCAATGGCGTTGGTGCAAGGTGAAAACAAAGTGGGAATTAAAATAAGGAAGAATGGATTTAGAGAGATTGAAATGAAATGAGGGAAGAGCTTTTTTTGGGCAAAGGGAAGGAGAAGGAAAAGTGTTTATATCAGAAAGAGAGAGAAAGAGAAAAAAAAAAAAAAAACTCTTTGGTTCTTTGGGGTTTAGATTTCTCTGTGCAAACTTAGCACAGATTCCGGCGCCAATTTGGGGAGCTCCTGCTCCCCGAATCTTCCTCTGTTTTGGAAAAAAAAAAACTAGTAGAAAGAAAAAAAGGAGAAGAATGTAAGAAGAAGAAGGAAGAAAAAAAAAATCCGACAGCCGCCGCCGCCGTTCGCCGGCAGCAGGCGGCAGCCGGCCATTGGAGTTGAAGAAGAAGGAGATGGGAAAGAAGGAAGAAGGAGATGGAGAAGAGAAAGAAGAAGGAGAAGAAAGAGGGAGATAAATAATTAAAAAAAATAATAAAATAAAAAAAAAATTATTATTTTAATATATTTTTATTTAAAAAGTTAAAAATCAATTTTTTTTAATTAAAATAATAGTGTCAAGCCTATTAAGATTTAACGGCAAAATGGGTGGCAGAGACCAAATTGTTCTTTATCTCAAACATTTGGTTGAGTTTGAAAAGACCAAAGTGTAACTTAATCCAAATCTCAGGGATCAAAAGTGTATTTTTCTCTAATTTTTATTATAATTATGTATGTGTATATATATATATATAACACATAAAATATTTATGATATGAAATAATAGAAAATTTGTTTTATATTGAAAATTATATTTTTTACACCCTCGTATAGTAACTTATGTGATTTCTTAAAGCTAACTAAGTTTGACTTTTGTTATTTCTTTTATAATTTTAAACATTAGTTGTTATAAATATGATAATATGGAAATTTTGAAGGGATAGGTAAATCTTCTAAAATGTTGGTAAACAAGTGTCTTCTTATATATGAAAGTAATTATAAACATTTTTTTTTAAGAAAAAAAGTAGCTATAAACTAAATGATACGTTTTTTTTTTTTTTTTTTGTTATATGTATTATTATTATTATACTTATTTTAATTATGTTATAATTGCAATTAATTAATATTCTCTTGTCAAAAAAAATTAATTAATATTCTGATGGTCAATCTAAACATAGTTTAACTGATTAAAGCATATGTCCTGACTAAGGTCGCCACACAGTGATCAAGTATTATTGTTATTGTTTCTATCAATATTTTACTTTCTAAAATTGTTTATTTTTGTTCTTAAATCATTTGTAGTGTGTGCGGACCGTTGTGGTCTTACATATAACCTTTTTGGTAAATGCGATATGCCACAGATGGGGGAAGCGACAATGGAATAGTAATGATTTGTCAAAGAATAATTGGTAA

mRNA sequence

ATGAAGTCTCAACTCAACTACACAATCCCTACAAATCTATTGGAATCTTCAAAGGAGCCAAAGGAGAAGAAGCCTTCAATGAAGTGGACTAAGTTAGATAATAATCATGTTGTTCCTACTTCTTATCTTCTCCTTATCCACATACTTTGCATTTTCGCACCATTCTATTTCAATTGGAATGCTTTTTGGGTTGCTTTAGCATTGGCTGTGGTTACCGGTATGACCATTACGGTCTCGTATCACAGAAACCTTTCACACAAAAGCTTCACACTCCCTAAATGGCTTGAGTATTTGCTTGCTTATTGTGCCGTTCATGCATTTCAGGGTGATCCAATCGGTTGGGTGAGCATCCATAGATATCATCATCAATATATAGATACTGAAAGAGATCCACACAGTCCTATTGGAGGATTTTGGTTTAGTTATATAACATGGCTATTTGATTTAAATACTTTGAATAAAAAAGTTCAATTCGACCATGTTAGTGATTTTCGAGAGACAAAAAAAGACACGGGAATATTCCAACATAAAAATCAGGATAATGTCAATGACTTGAAGAATCAACTCTTCTATAGGCTGATGAATGAAACTTACATTCTTCATCCAATTGCTCTAGCAGTCTTGTTGTATGCCATCGGAGGAGTTCCTTTTATCCTATGGGGATTGTGTGTGCGGACCGTTGTGGTCTTACATATAACCTTTTTGGTAAATGCGATATGCCACAGATGGGGGAAGCGACAATGGAATAGTAATGATTTGTCAAAGAATAATTGGTAA

Coding sequence (CDS)

ATGAAGTCTCAACTCAACTACACAATCCCTACAAATCTATTGGAATCTTCAAAGGAGCCAAAGGAGAAGAAGCCTTCAATGAAGTGGACTAAGTTAGATAATAATCATGTTGTTCCTACTTCTTATCTTCTCCTTATCCACATACTTTGCATTTTCGCACCATTCTATTTCAATTGGAATGCTTTTTGGGTTGCTTTAGCATTGGCTGTGGTTACCGGTATGACCATTACGGTCTCGTATCACAGAAACCTTTCACACAAAAGCTTCACACTCCCTAAATGGCTTGAGTATTTGCTTGCTTATTGTGCCGTTCATGCATTTCAGGGTGATCCAATCGGTTGGGTGAGCATCCATAGATATCATCATCAATATATAGATACTGAAAGAGATCCACACAGTCCTATTGGAGGATTTTGGTTTAGTTATATAACATGGCTATTTGATTTAAATACTTTGAATAAAAAAGTTCAATTCGACCATGTTAGTGATTTTCGAGAGACAAAAAAAGACACGGGAATATTCCAACATAAAAATCAGGATAATGTCAATGACTTGAAGAATCAACTCTTCTATAGGCTGATGAATGAAACTTACATTCTTCATCCAATTGCTCTAGCAGTCTTGTTGTATGCCATCGGAGGAGTTCCTTTTATCCTATGGGGATTGTGTGTGCGGACCGTTGTGGTCTTACATATAACCTTTTTGGTAAATGCGATATGCCACAGATGGGGGAAGCGACAATGGAATAGTAATGATTTGTCAAAGAATAATTGGTAA

Protein sequence

MKSQLNYTIPTNLLESSKEPKEKKPSMKWTKLDNNHVVPTSYLLLIHILCIFAPFYFNWNAFWVALALAVVTGMTITVSYHRNLSHKSFTLPKWLEYLLAYCAVHAFQGDPIGWVSIHRYHHQYIDTERDPHSPIGGFWFSYITWLFDLNTLNKKVQFDHVSDFRETKKDTGIFQHKNQDNVNDLKNQLFYRLMNETYILHPIALAVLLYAIGGVPFILWGLCVRTVVVLHITFLVNAICHRWGKRQWNSNDLSKNNW
Homology
BLAST of Spg014393 vs. NCBI nr
Match: XP_022980838.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 321.6 bits (823), Expect = 6.1e-84
Identity = 146/233 (62.66%), Postives = 184/233 (78.97%), Query Frame = 0

Query: 28  KWTKLDNNHVVPTSYLLLIHILCIFAPFYFNWNAFWVALALAVVTGMTITVSYHRNLSHK 87
           KWTKLD    +    L ++H LCIFAPFYFNW+AFWV+ AL V+TG++I+VSYHRNL+HK
Sbjct: 18  KWTKLDQYISIA---LFILHFLCIFAPFYFNWHAFWVSFALYVITGLSISVSYHRNLAHK 77

Query: 88  SFTLPKWLEYLLAYCAVHAFQGDPIGWVSIHRYHHQYIDTERDPHSPIGGFWFSYITWLF 147
           SF LPK LEY  AYCA HA QGDPI WVS HR HHQ++DTERDPHSPI GFWFS+I WLF
Sbjct: 78  SFELPKLLEYFFAYCAAHALQGDPIDWVSTHRCHHQFVDTERDPHSPIQGFWFSHILWLF 137

Query: 148 DLNTLNKKVQFDHVSDFRETKKDTGIF--QHKNQDNVNDLKNQLFYRLMNETYILHPIAL 207
           D  TL K+V   + +DF++ ++   I+  +H   DNV DL+ Q FYR +++TY+LH +A 
Sbjct: 138 DSYTLTKRVCPKYFTDFKKIERSIIIWFSKHGRPDNVRDLEKQAFYRFIHKTYLLHHVAF 197

Query: 208 AVLLYAIGGVPFILWGLCVRTVVVLHITFLVNAICHRWGKRQWNSNDLSKNNW 259
           AVLLY +GG+PF++WG CVRTVVVLHITF+VN++CH WGKR+WN+ DLS+NNW
Sbjct: 198 AVLLYEVGGLPFLIWGTCVRTVVVLHITFMVNSVCHIWGKRRWNTKDLSRNNW 247

BLAST of Spg014393 vs. NCBI nr
Match: XP_022155827.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 321.2 bits (822), Expect = 7.9e-84
Identity = 151/247 (61.13%), Postives = 187/247 (75.71%), Query Frame = 0

Query: 16  SSKEPKE--KKPSMKWTKLDNNHVVPTSYLLLIHILCIFAPFYFNWNAFWVALALAVVTG 75
           S +EPKE  K P   + +         +  +L+H+LCI APF+FNW AFWVA  LA  TG
Sbjct: 4   SKEEPKEMVKPPPSPFERRMLRRRTVFAPFILLHLLCILAPFHFNWKAFWVAFGLATTTG 63

Query: 76  MTITVSYHRNLSHKSFTLPKWLEYLLAYCAVHAFQGDPIGWVSIHRYHHQYIDTERDPHS 135
           + I+VSYHRNL+H+SF LPKWLEYLL YCA HAFQGDPI WVS HR HH+Y+DTERDPHS
Sbjct: 64  LMISVSYHRNLAHRSFKLPKWLEYLLVYCAAHAFQGDPIDWVSTHRCHHRYVDTERDPHS 123

Query: 136 PIGGFWFSYITWLFDLNTLNKKVQFDHVSDFRETKKDTGI--FQHKNQDNVNDLKNQLFY 195
           P  GFWFS+ITWLFD  TL KKV  ++ + F + +K+  +   +H N DNV DL+ Q+FY
Sbjct: 124 PTQGFWFSHITWLFDSYTLTKKVCPNYSNGFGKIEKNIFMLFLKHGNPDNVPDLEKQVFY 183

Query: 196 RLMNETYILHPIALAVLLYAIGGVPFILWGLCVRTVVVLHITFLVNAICHRWGKRQWNSN 255
           R M +TY LHPIALAVLLYA+GGVPFI+WG+CVR  V LHITF+VN++CH WG++QW++ 
Sbjct: 184 RFMQKTYFLHPIALAVLLYAVGGVPFIIWGMCVRITVSLHITFMVNSVCHIWGEQQWDTG 243

Query: 256 DLSKNNW 259
           DLSKNNW
Sbjct: 244 DLSKNNW 250

BLAST of Spg014393 vs. NCBI nr
Match: XP_022155833.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 320.1 bits (819), Expect = 1.8e-83
Identity = 150/251 (59.76%), Postives = 190/251 (75.70%), Query Frame = 0

Query: 10  PTNLLESSKEPKEKKPSMKWTKLDNNHVVPTSYLLLIHILCIFAPFYFNWNAFWVALALA 69
           P +++     P E+K   KWTKLD N  V +S+ +L H+LCI APF FNW AFWV L + 
Sbjct: 8   PKDMVRLPLRPFERK---KWTKLDKN--VASSFFIL-HLLCILAPFNFNWGAFWVFLVVG 67

Query: 70  VVTGMTITVSYHRNLSHKSFTLPKWLEYLLAYCAVHAFQGDPIGWVSIHRYHHQYIDTER 129
            +TGM I+VSYHRNL+H+SF LPKWLEYLLAYCA HAFQGDPI WVS HR HH+Y+DTER
Sbjct: 68  SLTGMAISVSYHRNLAHRSFKLPKWLEYLLAYCAAHAFQGDPIDWVSTHRSHHRYVDTER 127

Query: 130 DPHSPIGGFWFSYITWLFDLNTLNKKVQFDHVSDFRETKKD--TGIFQHKNQDNVNDLKN 189
           DPHSPI GFWFS ITWLFD  +L  K   ++  DFR+ +++  TG  ++ N  NV DL+ 
Sbjct: 128 DPHSPINGFWFSQITWLFDSYSLTTKNCPNYFGDFRKIERNIFTGSLKYANPSNVPDLEK 187

Query: 190 QLFYRLMNETYILHPIALAVLLYAIGGVPFILWGLCVRTVVVLHITFLVNAICHRWGKRQ 249
           Q FYR M +TY+LH +AL +LLY IGGVP+I+WG+C+R VV  H+T +VN++CH WGK+Q
Sbjct: 188 QAFYRFMQKTYLLHLLALTILLYVIGGVPYIIWGMCMRIVVSFHLTSMVNSVCHMWGKKQ 247

Query: 250 WNSNDLSKNNW 259
           WN++DLSKNNW
Sbjct: 248 WNTDDLSKNNW 252

BLAST of Spg014393 vs. NCBI nr
Match: XP_022155832.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 319.3 bits (817), Expect = 3.0e-83
Identity = 151/252 (59.92%), Postives = 190/252 (75.40%), Query Frame = 0

Query: 16  SSKEPKE--KKP-----SMKWTKLDNNHVVPTSYLLLIHILCIFAPFYFNWNAFWVALAL 75
           S +EPKE  K P        WTKLD N     S L L+H+LCI APF+FNW AFW+ L L
Sbjct: 4   SKQEPKEMVKLPLRPFDRKTWTKLDKN---IASSLFLLHLLCILAPFHFNWGAFWIFLVL 63

Query: 76  AVVTGMTITVSYHRNLSHKSFTLPKWLEYLLAYCAVHAFQGDPIGWVSIHRYHHQYIDTE 135
             +TG+ I+VSYH+NL+H+SF LPKWLEYLLAY A HAFQGDPI WVS HR HHQY+DTE
Sbjct: 64  TNLTGIAISVSYHKNLAHRSFKLPKWLEYLLAYYAAHAFQGDPIDWVSTHRSHHQYVDTE 123

Query: 136 RDPHSPIGGFWFSYITWLFDLNTLNKKVQFDHVSDFRETKKDTGI--FQHKNQDNVNDLK 195
           RDPHSP  GFWFS+I WLFD  TL K+V  ++ +DF+E +++  +   +H N  NV+DL+
Sbjct: 124 RDPHSPTNGFWFSHIIWLFDSYTLTKRVCPNYFNDFQEIERNIFMTYLKHGNPSNVHDLE 183

Query: 196 NQLFYRLMNETYILHPIALAVLLYAIGGVPFILWGLCVRTVVVLHITFLVNAICHRWGKR 255
            Q FYR M +TY+LH  ALA+LLY +GGVP+I+WG+C+R VV LH+TF+VN++CH WGK+
Sbjct: 184 KQAFYRFMQKTYLLHIFALAILLYFVGGVPYIIWGMCMRIVVTLHLTFMVNSVCHIWGKQ 243

Query: 256 QWNSNDLSKNNW 259
           QWN+ DLSKNNW
Sbjct: 244 QWNTRDLSKNNW 252

BLAST of Spg014393 vs. NCBI nr
Match: XP_023526679.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 317.4 bits (812), Expect = 1.1e-82
Identity = 143/233 (61.37%), Postives = 184/233 (78.97%), Query Frame = 0

Query: 28  KWTKLDNNHVVPTSYLLLIHILCIFAPFYFNWNAFWVALALAVVTGMTITVSYHRNLSHK 87
           KWTKLD    +    L ++HILCIFAPF+FNW AFWV+ AL V+TG++I+VSYHRNL+HK
Sbjct: 18  KWTKLDQYISIA---LFILHILCIFAPFHFNWRAFWVSFALYVITGLSISVSYHRNLAHK 77

Query: 88  SFTLPKWLEYLLAYCAVHAFQGDPIGWVSIHRYHHQYIDTERDPHSPIGGFWFSYITWLF 147
           SF LPK LEY  AYCA HA QGDPI WVS HR HHQ++DTERDPHSP+ GFWFS+I WLF
Sbjct: 78  SFELPKLLEYFFAYCAAHALQGDPIDWVSTHRCHHQFVDTERDPHSPVQGFWFSHILWLF 137

Query: 148 DLNTLNKKVQFDHVSDFRETKKDTGIF--QHKNQDNVNDLKNQLFYRLMNETYILHPIAL 207
           D  TL K+V   + ++F++ ++   I+  +H   DNV DL+ Q FYR +++TY+LH +A 
Sbjct: 138 DSYTLTKRVCPKYFTNFKKIERSIIIWFSKHGRPDNVRDLEKQAFYRFIHKTYLLHHVAF 197

Query: 208 AVLLYAIGGVPFILWGLCVRTVVVLHITFLVNAICHRWGKRQWNSNDLSKNNW 259
           AVLLY +GG+PF++WG+CVR VV+LHITF+VN++CH WGKR+WN+ DLSKNNW
Sbjct: 198 AVLLYEVGGLPFLIWGMCVRIVVLLHITFMVNSVCHIWGKRRWNTKDLSKNNW 247

BLAST of Spg014393 vs. ExPASy Swiss-Prot
Match: Q949X0 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3 PE=1 SV=2)

HSP 1 Score: 259.2 bits (661), Expect = 4.9e-68
Identity = 124/232 (53.45%), Postives = 158/232 (68.10%), Query Frame = 0

Query: 28  KWTKLDNNHVVPTSYLLLIHILCIFAPFYFNWNAFWVALALAVVTGMT-ITVSYHRNLSH 87
           +W  +D   V   + +L +H+L + APF FNW A  VA  L +VTG+  IT+S+HRNLSH
Sbjct: 97  EWKAMDFGAV---AVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSFHRNLSH 156

Query: 88  KSFTLPKWLEYLLAYCAVHAFQGDPIGWVSIHRYHHQYIDTERDPHSPIGGFWFSYITWL 147
           K+F LPKWLEYL AYC   A QG+PI WVS HRYHHQ+ D++RDPHSP+ GFWFS++ W+
Sbjct: 157 KAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWFSHMNWM 216

Query: 148 FDLNTLNKKVQFDHVSDFRETKKDTGIFQHKNQDNVNDLKNQLFYRLMNETYILHPIALA 207
           FD NT+ ++                        +NV DL+ Q FYR +  TYILHP+ALA
Sbjct: 217 FDTNTITQRC--------------------GEPNNVGDLEKQPFYRFLRTTYILHPLALA 276

Query: 208 VLLYAIGGVPFILWGLCVRTVVVLHITFLVNAICHRWGKRQWNSNDLSKNNW 259
           V LYA+GG PFI+WG+ VR V V HIT+LVN+ CH WGK+ WN+ DLSKNNW
Sbjct: 277 VALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSKNNW 305

BLAST of Spg014393 vs. ExPASy Swiss-Prot
Match: Q9LVZ3 (Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3.2 PE=2 SV=3)

HSP 1 Score: 236.9 bits (603), Expect = 2.6e-61
Identity = 111/230 (48.26%), Postives = 149/230 (64.78%), Query Frame = 0

Query: 29  WTKLDNNHVVPTSYLLLIHILCIFAPFYFNWNAFWVALALAVVTGMTITVSYHRNLSHKS 88
           WT  D + +V     +  H+L + APFYF+W AFWV   L  + G+ IT+SYHRNLSH+S
Sbjct: 91  WTSWDVSKLV---IFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGICITLSYHRNLSHRS 150

Query: 89  FTLPKWLEYLLAYCAVHAFQGDPIGWVSIHRYHHQYIDTERDPHSPIGGFWFSYITWLFD 148
           F LPKWLEYL AY  V AFQGDPI WVS HRYHH++ +T+RDPHSP  GFWFS++ W+FD
Sbjct: 151 FDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQGFWFSHMAWIFD 210

Query: 149 LNTLNKKVQFDHVSDFRETKKDTGIFQHKNQDNVNDLKNQLFYRLMNETYILHPIALAVL 208
            +++ +                        ++NV+DL  Q FYR +  T +LH +A + L
Sbjct: 211 TSSILENC--------------------GGEENVDDLVRQPFYRFLQRTVLLHMMAYSFL 270

Query: 209 LYAIGGVPFILWGLCVRTVVVLHITFLVNAICHRWGKRQWNSNDLSKNNW 259
            Y  GG+P ++WG+ +   V LH+TFLVN++CH WG R WN++D SKNNW
Sbjct: 271 FYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSDFSKNNW 297

BLAST of Spg014393 vs. ExPASy Swiss-Prot
Match: P0DOW2 (Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 2.2e-60
Identity = 110/240 (45.83%), Postives = 148/240 (61.67%), Query Frame = 0

Query: 21  KEKKPSMKWT-KLDNNHVVPTSYLLLIHILCIFAPFYFNWNAFWVALALAVVTGM-TITV 80
           KEK   + W  K ++  V    ++  +H+LC+FAP  F+W +FWV  AL  + G+   T+
Sbjct: 28  KEKTKKITWVRKWNSTDVFHILFIGGLHVLCLFAPSTFSWKSFWVCFALYAICGVFGTTL 87

Query: 81  SYHRNLSHKSFTLPKWLEYLLAYCAVHAFQGDPIGWVSIHRYHHQYIDTERDPHSPIGGF 140
           S+HRNL+H+SF LPK+LEY  AY  +HA QGDP+ WVS HRYHH+Y DT  DPHSPI GF
Sbjct: 88  SFHRNLTHRSFKLPKYLEYFFAYVGLHALQGDPVWWVSTHRYHHKYTDTYLDPHSPIEGF 147

Query: 141 WFSYITWLFDLNTLNKKVQFDHVSDFRETKKDTGIFQHKNQDNVNDLKNQLFYRLMNETY 200
           WF +I WLFD   + +K                        +N  DL  Q +YR +  T+
Sbjct: 148 WFCHIFWLFDSKYIIEKC--------------------GRYENAGDLMKQSYYRFLERTF 207

Query: 201 ILHPIALAVLLYAIGGVPFILWGLCVRTVVVLHITFLVNAICHRWGKRQWNSNDLSKNNW 259
           + H    A LLY  GG PFI+WG+ VRT++  H+++LVN++CHRWG R WN+ DLS NNW
Sbjct: 208 VYHVYLQAALLYLFGGFPFIVWGMAVRTILGFHLSWLVNSVCHRWGNRPWNTGDLSTNNW 247

BLAST of Spg014393 vs. ExPASy Swiss-Prot
Match: Q9SID2 (Delta-9 acyl-lipid desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=ADS2 PE=2 SV=2)

HSP 1 Score: 229.6 bits (584), Expect = 4.1e-59
Identity = 107/231 (46.32%), Postives = 142/231 (61.47%), Query Frame = 0

Query: 28  KWTKLDNNHVVPTSYLLLIHILCIFAPFYFNWNAFWVALALAVVTGMTITVSYHRNLSHK 87
           +W +LD    V  S    +H L + APFYF W+A WV      + G+ ITVSYHRNL+H+
Sbjct: 35  RWRRLD---YVKFSASFTVHSLALLAPFYFTWSALWVTFLFYTIGGLGITVSYHRNLAHR 94

Query: 88  SFTLPKWLEYLLAYCAVHAFQGDPIGWVSIHRYHHQYIDTERDPHSPIGGFWFSYITWLF 147
           SF +PKWLEYLLAYCA+ A QGDPI WVS HRYHHQ+ D+ERDPHSP  GFWFS++ W++
Sbjct: 95  SFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGFWFSHLLWIY 154

Query: 148 DLNTLNKKVQFDHVSDFRETKKDTGIFQHKNQDNVNDLKNQLFYRLMNETYILHPIALAV 207
           D   L  K                       + NV DLK Q FYR + +T + H + L  
Sbjct: 155 DSAYLVSKC--------------------GRRANVEDLKRQWFYRFLQKTVLFHILGLGF 214

Query: 208 LLYAIGGVPFILWGLCVRTVVVLHITFLVNAICHRWGKRQWNSNDLSKNNW 259
            L+ +GG+ F+ WG+ V   + +H+T L+N++CH WG R W +ND S+N W
Sbjct: 215 FLFYLGGMSFVTWGMGVGAALEVHVTCLINSLCHIWGTRTWKTNDTSRNVW 242

BLAST of Spg014393 vs. ExPASy Swiss-Prot
Match: P0DOW3 (Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 1.7e-57
Identity = 109/243 (44.86%), Postives = 150/243 (61.73%), Query Frame = 0

Query: 21  KEKKPSMKWTKLDNN----HVVPTSYLLLIHILCIFAPFYFNWNAFWVALALAVVTGM-T 80
           K+K   + W ++ N+    H++    L   H+LC+ APF F+W+AFW++L L  V G+  
Sbjct: 25  KDKTKKITWVRVWNSTDIFHIILVGGL---HVLCLSAPFTFSWSAFWLSLTLYAVCGVFG 84

Query: 81  ITVSYHRNLSHKSFTLPKWLEYLLAYCAVHAFQGDPIGWVSIHRYHHQYIDTERDPHSPI 140
            T+SYHRNL+H+SF LPK+LEY  AY  +HA QGDP+ WVS HRYHH++ DT  DPHSPI
Sbjct: 85  TTLSYHRNLTHRSFKLPKYLEYFFAYVGLHALQGDPVWWVSTHRYHHKFTDTYLDPHSPI 144

Query: 141 GGFWFSYITWLFDLNTLNKKVQFDHVSDFRETKKDTGIFQHKNQDNVNDLKNQLFYRLMN 200
            GFWFS+I WLFD   +                    + +    +N  DL  Q +YR + 
Sbjct: 145 EGFWFSHIFWLFDSKYI--------------------LEECGRYENAGDLLKQSYYRFLE 204

Query: 201 ETYILHPIALAVLLYAIGGVPFILWGLCVRTVVVLHITFLVNAICHRWGKRQWNSNDLSK 259
            T++ H    A LLY  GG PFI+WG+ VRTV   H ++LVN++CHR+G + W++ DLS 
Sbjct: 205 RTFVFHVYLQAALLYMFGGFPFIVWGMAVRTVWGFHASWLVNSVCHRYGHQAWDTGDLST 244

BLAST of Spg014393 vs. ExPASy TrEMBL
Match: A0A6J1J0F3 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111480111 PE=3 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 2.9e-84
Identity = 146/233 (62.66%), Postives = 184/233 (78.97%), Query Frame = 0

Query: 28  KWTKLDNNHVVPTSYLLLIHILCIFAPFYFNWNAFWVALALAVVTGMTITVSYHRNLSHK 87
           KWTKLD    +    L ++H LCIFAPFYFNW+AFWV+ AL V+TG++I+VSYHRNL+HK
Sbjct: 18  KWTKLDQYISIA---LFILHFLCIFAPFYFNWHAFWVSFALYVITGLSISVSYHRNLAHK 77

Query: 88  SFTLPKWLEYLLAYCAVHAFQGDPIGWVSIHRYHHQYIDTERDPHSPIGGFWFSYITWLF 147
           SF LPK LEY  AYCA HA QGDPI WVS HR HHQ++DTERDPHSPI GFWFS+I WLF
Sbjct: 78  SFELPKLLEYFFAYCAAHALQGDPIDWVSTHRCHHQFVDTERDPHSPIQGFWFSHILWLF 137

Query: 148 DLNTLNKKVQFDHVSDFRETKKDTGIF--QHKNQDNVNDLKNQLFYRLMNETYILHPIAL 207
           D  TL K+V   + +DF++ ++   I+  +H   DNV DL+ Q FYR +++TY+LH +A 
Sbjct: 138 DSYTLTKRVCPKYFTDFKKIERSIIIWFSKHGRPDNVRDLEKQAFYRFIHKTYLLHHVAF 197

Query: 208 AVLLYAIGGVPFILWGLCVRTVVVLHITFLVNAICHRWGKRQWNSNDLSKNNW 259
           AVLLY +GG+PF++WG CVRTVVVLHITF+VN++CH WGKR+WN+ DLS+NNW
Sbjct: 198 AVLLYEVGGLPFLIWGTCVRTVVVLHITFMVNSVCHIWGKRRWNTKDLSRNNW 247

BLAST of Spg014393 vs. ExPASy TrEMBL
Match: A0A6J1DQF3 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111022855 PE=3 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 3.8e-84
Identity = 151/247 (61.13%), Postives = 187/247 (75.71%), Query Frame = 0

Query: 16  SSKEPKE--KKPSMKWTKLDNNHVVPTSYLLLIHILCIFAPFYFNWNAFWVALALAVVTG 75
           S +EPKE  K P   + +         +  +L+H+LCI APF+FNW AFWVA  LA  TG
Sbjct: 4   SKEEPKEMVKPPPSPFERRMLRRRTVFAPFILLHLLCILAPFHFNWKAFWVAFGLATTTG 63

Query: 76  MTITVSYHRNLSHKSFTLPKWLEYLLAYCAVHAFQGDPIGWVSIHRYHHQYIDTERDPHS 135
           + I+VSYHRNL+H+SF LPKWLEYLL YCA HAFQGDPI WVS HR HH+Y+DTERDPHS
Sbjct: 64  LMISVSYHRNLAHRSFKLPKWLEYLLVYCAAHAFQGDPIDWVSTHRCHHRYVDTERDPHS 123

Query: 136 PIGGFWFSYITWLFDLNTLNKKVQFDHVSDFRETKKDTGI--FQHKNQDNVNDLKNQLFY 195
           P  GFWFS+ITWLFD  TL KKV  ++ + F + +K+  +   +H N DNV DL+ Q+FY
Sbjct: 124 PTQGFWFSHITWLFDSYTLTKKVCPNYSNGFGKIEKNIFMLFLKHGNPDNVPDLEKQVFY 183

Query: 196 RLMNETYILHPIALAVLLYAIGGVPFILWGLCVRTVVVLHITFLVNAICHRWGKRQWNSN 255
           R M +TY LHPIALAVLLYA+GGVPFI+WG+CVR  V LHITF+VN++CH WG++QW++ 
Sbjct: 184 RFMQKTYFLHPIALAVLLYAVGGVPFIIWGMCVRITVSLHITFMVNSVCHIWGEQQWDTG 243

Query: 256 DLSKNNW 259
           DLSKNNW
Sbjct: 244 DLSKNNW 250

BLAST of Spg014393 vs. ExPASy TrEMBL
Match: A0A6J1DNZ3 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111022863 PE=3 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 8.6e-84
Identity = 150/251 (59.76%), Postives = 190/251 (75.70%), Query Frame = 0

Query: 10  PTNLLESSKEPKEKKPSMKWTKLDNNHVVPTSYLLLIHILCIFAPFYFNWNAFWVALALA 69
           P +++     P E+K   KWTKLD N  V +S+ +L H+LCI APF FNW AFWV L + 
Sbjct: 8   PKDMVRLPLRPFERK---KWTKLDKN--VASSFFIL-HLLCILAPFNFNWGAFWVFLVVG 67

Query: 70  VVTGMTITVSYHRNLSHKSFTLPKWLEYLLAYCAVHAFQGDPIGWVSIHRYHHQYIDTER 129
            +TGM I+VSYHRNL+H+SF LPKWLEYLLAYCA HAFQGDPI WVS HR HH+Y+DTER
Sbjct: 68  SLTGMAISVSYHRNLAHRSFKLPKWLEYLLAYCAAHAFQGDPIDWVSTHRSHHRYVDTER 127

Query: 130 DPHSPIGGFWFSYITWLFDLNTLNKKVQFDHVSDFRETKKD--TGIFQHKNQDNVNDLKN 189
           DPHSPI GFWFS ITWLFD  +L  K   ++  DFR+ +++  TG  ++ N  NV DL+ 
Sbjct: 128 DPHSPINGFWFSQITWLFDSYSLTTKNCPNYFGDFRKIERNIFTGSLKYANPSNVPDLEK 187

Query: 190 QLFYRLMNETYILHPIALAVLLYAIGGVPFILWGLCVRTVVVLHITFLVNAICHRWGKRQ 249
           Q FYR M +TY+LH +AL +LLY IGGVP+I+WG+C+R VV  H+T +VN++CH WGK+Q
Sbjct: 188 QAFYRFMQKTYLLHLLALTILLYVIGGVPYIIWGMCMRIVVSFHLTSMVNSVCHMWGKKQ 247

Query: 250 WNSNDLSKNNW 259
           WN++DLSKNNW
Sbjct: 248 WNTDDLSKNNW 252

BLAST of Spg014393 vs. ExPASy TrEMBL
Match: A0A6J1DQF8 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111022861 PE=3 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 1.5e-83
Identity = 151/252 (59.92%), Postives = 190/252 (75.40%), Query Frame = 0

Query: 16  SSKEPKE--KKP-----SMKWTKLDNNHVVPTSYLLLIHILCIFAPFYFNWNAFWVALAL 75
           S +EPKE  K P        WTKLD N     S L L+H+LCI APF+FNW AFW+ L L
Sbjct: 4   SKQEPKEMVKLPLRPFDRKTWTKLDKN---IASSLFLLHLLCILAPFHFNWGAFWIFLVL 63

Query: 76  AVVTGMTITVSYHRNLSHKSFTLPKWLEYLLAYCAVHAFQGDPIGWVSIHRYHHQYIDTE 135
             +TG+ I+VSYH+NL+H+SF LPKWLEYLLAY A HAFQGDPI WVS HR HHQY+DTE
Sbjct: 64  TNLTGIAISVSYHKNLAHRSFKLPKWLEYLLAYYAAHAFQGDPIDWVSTHRSHHQYVDTE 123

Query: 136 RDPHSPIGGFWFSYITWLFDLNTLNKKVQFDHVSDFRETKKDTGI--FQHKNQDNVNDLK 195
           RDPHSP  GFWFS+I WLFD  TL K+V  ++ +DF+E +++  +   +H N  NV+DL+
Sbjct: 124 RDPHSPTNGFWFSHIIWLFDSYTLTKRVCPNYFNDFQEIERNIFMTYLKHGNPSNVHDLE 183

Query: 196 NQLFYRLMNETYILHPIALAVLLYAIGGVPFILWGLCVRTVVVLHITFLVNAICHRWGKR 255
            Q FYR M +TY+LH  ALA+LLY +GGVP+I+WG+C+R VV LH+TF+VN++CH WGK+
Sbjct: 184 KQAFYRFMQKTYLLHIFALAILLYFVGGVPYIIWGMCMRIVVTLHLTFMVNSVCHIWGKQ 243

Query: 256 QWNSNDLSKNNW 259
           QWN+ DLSKNNW
Sbjct: 244 QWNTRDLSKNNW 252

BLAST of Spg014393 vs. ExPASy TrEMBL
Match: A0A6J1DIY2 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111020911 PE=3 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 5.5e-83
Identity = 151/256 (58.98%), Postives = 190/256 (74.22%), Query Frame = 0

Query: 13  LLESSKEPK--EKKP----SMKWTKLDNNHVVPTSYLLLIHILCIFAPFYFNWNAFWVAL 72
           ++++SKEPK   K P      KWTK+D  +      LL +H+LCIFAPF FNW+AFW+A 
Sbjct: 2   VMDASKEPKIIVKSPMSFGRRKWTKMDKTN---ACILLFLHVLCIFAPFQFNWSAFWIAF 61

Query: 73  ALAVVTGM-TITVSYHRNLSHKSFTLPKWLEYLLAYCAVHAFQGDPIGWVSIHRYHHQYI 132
           AL ++TG+  IT+SYHRNLSH+SF LPKWLEYL AYC V + QGDPI WVS HRYHHQ++
Sbjct: 62  ALYIITGLFGITISYHRNLSHRSFKLPKWLEYLFAYCGVQSLQGDPIYWVSTHRYHHQFV 121

Query: 133 DTERDPHSPIGGFWFSYITWLFDLNTLNKKVQFDHVSDFRE---TKKDTGIFQHKNQDNV 192
           DTE+DPHSPI GFWFSYI W+FD NTL  K     +S+F+E         I +H   DNV
Sbjct: 122 DTEKDPHSPIQGFWFSYIFWIFDSNTLTNK-----MSNFQERPDRSTSVMIMKHNRLDNV 181

Query: 193 NDLKNQLFYRLMNETYILHPIALAVLLYAIGGVPFILWGLCVRTVVVLHITFLVNAICHR 252
            DLK Q FY  ++ TYILHPIALAV+LYA GG+PF++WG+CVR+V++LH T+LVN+ICH 
Sbjct: 182 GDLKKQTFYTFIHNTYILHPIALAVILYAGGGIPFLIWGMCVRSVMLLHATYLVNSICHL 241

Query: 253 WGKRQWNSNDLSKNNW 259
           WGK+ W + DLS+NNW
Sbjct: 242 WGKQPWKTGDLSRNNW 249

BLAST of Spg014393 vs. TAIR 10
Match: AT3G15850.1 (fatty acid desaturase 5 )

HSP 1 Score: 259.2 bits (661), Expect = 3.5e-69
Identity = 124/232 (53.45%), Postives = 158/232 (68.10%), Query Frame = 0

Query: 28  KWTKLDNNHVVPTSYLLLIHILCIFAPFYFNWNAFWVALALAVVTGMT-ITVSYHRNLSH 87
           +W  +D   V   + +L +H+L + APF FNW A  VA  L +VTG+  IT+S+HRNLSH
Sbjct: 97  EWKAMDFGAV---AVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSFHRNLSH 156

Query: 88  KSFTLPKWLEYLLAYCAVHAFQGDPIGWVSIHRYHHQYIDTERDPHSPIGGFWFSYITWL 147
           K+F LPKWLEYL AYC   A QG+PI WVS HRYHHQ+ D++RDPHSP+ GFWFS++ W+
Sbjct: 157 KAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWFSHMNWM 216

Query: 148 FDLNTLNKKVQFDHVSDFRETKKDTGIFQHKNQDNVNDLKNQLFYRLMNETYILHPIALA 207
           FD NT+ ++                        +NV DL+ Q FYR +  TYILHP+ALA
Sbjct: 217 FDTNTITQRC--------------------GEPNNVGDLEKQPFYRFLRTTYILHPLALA 276

Query: 208 VLLYAIGGVPFILWGLCVRTVVVLHITFLVNAICHRWGKRQWNSNDLSKNNW 259
           V LYA+GG PFI+WG+ VR V V HIT+LVN+ CH WGK+ WN+ DLSKNNW
Sbjct: 277 VALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSKNNW 305

BLAST of Spg014393 vs. TAIR 10
Match: AT3G15870.1 (Fatty acid desaturase family protein )

HSP 1 Score: 236.9 bits (603), Expect = 1.8e-62
Identity = 111/230 (48.26%), Postives = 149/230 (64.78%), Query Frame = 0

Query: 29  WTKLDNNHVVPTSYLLLIHILCIFAPFYFNWNAFWVALALAVVTGMTITVSYHRNLSHKS 88
           WT  D + +V     +  H+L + APFYF+W AFWV   L  + G+ IT+SYHRNLSH+S
Sbjct: 91  WTSWDVSKLV---IFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGICITLSYHRNLSHRS 150

Query: 89  FTLPKWLEYLLAYCAVHAFQGDPIGWVSIHRYHHQYIDTERDPHSPIGGFWFSYITWLFD 148
           F LPKWLEYL AY  V AFQGDPI WVS HRYHH++ +T+RDPHSP  GFWFS++ W+FD
Sbjct: 151 FDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQGFWFSHMAWIFD 210

Query: 149 LNTLNKKVQFDHVSDFRETKKDTGIFQHKNQDNVNDLKNQLFYRLMNETYILHPIALAVL 208
            +++ +                        ++NV+DL  Q FYR +  T +LH +A + L
Sbjct: 211 TSSILENC--------------------GGEENVDDLVRQPFYRFLQRTVLLHMMAYSFL 270

Query: 209 LYAIGGVPFILWGLCVRTVVVLHITFLVNAICHRWGKRQWNSNDLSKNNW 259
            Y  GG+P ++WG+ +   V LH+TFLVN++CH WG R WN++D SKNNW
Sbjct: 271 FYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSDFSKNNW 297

BLAST of Spg014393 vs. TAIR 10
Match: AT2G31360.1 (16:0delta9 desaturase 2 )

HSP 1 Score: 229.6 bits (584), Expect = 2.9e-60
Identity = 107/231 (46.32%), Postives = 142/231 (61.47%), Query Frame = 0

Query: 28  KWTKLDNNHVVPTSYLLLIHILCIFAPFYFNWNAFWVALALAVVTGMTITVSYHRNLSHK 87
           +W +LD    V  S    +H L + APFYF W+A WV      + G+ ITVSYHRNL+H+
Sbjct: 35  RWRRLD---YVKFSASFTVHSLALLAPFYFTWSALWVTFLFYTIGGLGITVSYHRNLAHR 94

Query: 88  SFTLPKWLEYLLAYCAVHAFQGDPIGWVSIHRYHHQYIDTERDPHSPIGGFWFSYITWLF 147
           SF +PKWLEYLLAYCA+ A QGDPI WVS HRYHHQ+ D+ERDPHSP  GFWFS++ W++
Sbjct: 95  SFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGFWFSHLLWIY 154

Query: 148 DLNTLNKKVQFDHVSDFRETKKDTGIFQHKNQDNVNDLKNQLFYRLMNETYILHPIALAV 207
           D   L  K                       + NV DLK Q FYR + +T + H + L  
Sbjct: 155 DSAYLVSKC--------------------GRRANVEDLKRQWFYRFLQKTVLFHILGLGF 214

Query: 208 LLYAIGGVPFILWGLCVRTVVVLHITFLVNAICHRWGKRQWNSNDLSKNNW 259
            L+ +GG+ F+ WG+ V   + +H+T L+N++CH WG R W +ND S+N W
Sbjct: 215 FLFYLGGMSFVTWGMGVGAALEVHVTCLINSLCHIWGTRTWKTNDTSRNVW 242

BLAST of Spg014393 vs. TAIR 10
Match: AT1G06080.1 (delta 9 desaturase 1 )

HSP 1 Score: 223.8 bits (569), Expect = 1.6e-58
Identity = 110/243 (45.27%), Postives = 144/243 (59.26%), Query Frame = 0

Query: 19  EPKEKKPSM---KWTKLDNNHVVPTSYLLLIHILCIFAPFYFNWNAFWVALALAVVTGMT 78
           E   KK +M   KW +LD   +V     L +H LC+ APF F W A  VAL +  V G+ 
Sbjct: 21  EMGRKKRAMWERKWKRLD---IVKAFASLFVHFLCLLAPFNFTWPALRVALIVYTVGGLG 80

Query: 79  ITVSYHRNLSHKSFTLPKWLEYLLAYCAVHAFQGDPIGWVSIHRYHHQYIDTERDPHSPI 138
           ITVSYHRNL+H+SF +PKWLEY  AYC + A QGDPI WVS HRYHHQ+ D++RDPHSP 
Sbjct: 81  ITVSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDSDRDPHSPN 140

Query: 139 GGFWFSYITWLFDLNTLNKKVQFDHVSDFRETKKDTGIFQHKNQDNVNDLKNQLFYRLMN 198
            GFWFS++ WLFD   L +K                       + NV DLK Q +Y+ + 
Sbjct: 141 EGFWFSHLLWLFDTGYLVEKC--------------------GRRTNVEDLKRQWYYKFLQ 200

Query: 199 ETYILHPIALAVLLYAIGGVPFILWGLCVRTVVVLHITFLVNAICHRWGKRQWNSNDLSK 258
            T + H +    LLY  GG+ F+ WG+ +   +  H+T L+N++CH WG R W +ND S+
Sbjct: 201 RTVLYHILTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCLINSLCHVWGSRTWKTNDTSR 240

BLAST of Spg014393 vs. TAIR 10
Match: AT1G06120.1 (Fatty acid desaturase family protein )

HSP 1 Score: 220.3 bits (560), Expect = 1.8e-57
Identity = 107/246 (43.50%), Postives = 150/246 (60.98%), Query Frame = 0

Query: 15  ESSKEPKEKKPSM--KWTKLDNNHVVPTSYLLLIHILCIFAPFYFNWNAFWVALALAVVT 74
           +S    KEK+  +  KWT++D   V+  S +  +H+LC+ APF + W AF  A  + + T
Sbjct: 12  QSKAVRKEKRAFLFRKWTRVD---VMRVSAVGAVHLLCLLAPFNYTWEAFRFAAMVGIST 71

Query: 75  GMTITVSYHRNLSHKSFTLPKWLEYLLAYCAVHAFQGDPIGWVSIHRYHHQYIDTERDPH 134
            ++IT SYHRNL+H+SF LPKWLEY  AY A+ A QG PI WVS HR+HHQ+ D++RDPH
Sbjct: 72  NLSITFSYHRNLTHRSFKLPKWLEYPFAYSALFALQGHPIDWVSTHRFHHQFTDSDRDPH 131

Query: 135 SPIGGFWFSYITWLFDLNTLNKKVQFDHVSDFRETKKDTGIFQHKNQDNVNDLKNQLFYR 194
           SPI GFWFS++ W+FD + + +K                       +DNV DLK Q FYR
Sbjct: 132 SPIEGFWFSHVFWIFDTSYIREKC--------------------GGRDNVMDLKQQWFYR 191

Query: 195 LMNETYILHPIALAVLLYAIGGVPFILWGLCVRTVVVLHITFLVNAICHRWGKRQWNSND 254
            +  T  LH +   +L+Y  GG+P++ W + V   +  H T+L+N+ CH WG R WN+ D
Sbjct: 192 FLQNTIGLHILTFWILVYLWGGLPYLTWSVGVGGAIGYHATWLINSACHIWGSRAWNTKD 234

Query: 255 LSKNNW 259
            S+N W
Sbjct: 252 TSRNIW 234

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022980838.16.1e-8462.66palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [C... [more]
XP_022155827.17.9e-8461.13palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [M... [more]
XP_022155833.11.8e-8359.76palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [M... [more]
XP_022155832.13.0e-8359.92palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [M... [more]
XP_023526679.11.1e-8261.37palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like is... [more]
Match NameE-valueIdentityDescription
Q949X04.9e-6853.45Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arab... [more]
Q9LVZ32.6e-6148.26Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana OX=3702 ... [more]
P0DOW22.2e-6045.83Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1[more]
Q9SID24.1e-5946.32Delta-9 acyl-lipid desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=ADS2 PE=2 SV=... [more]
P0DOW31.7e-5744.86Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1J0F32.9e-8462.66palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1DQF33.8e-8461.13palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1DNZ38.6e-8459.76palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1DQF81.5e-8359.92palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1DIY25.5e-8358.98palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
Match NameE-valueIdentityDescription
AT3G15850.13.5e-6953.45fatty acid desaturase 5 [more]
AT3G15870.11.8e-6248.26Fatty acid desaturase family protein [more]
AT2G31360.12.9e-6046.3216:0delta9 desaturase 2 [more]
AT1G06080.11.6e-5845.27delta 9 desaturase 1 [more]
AT1G06120.11.8e-5743.50Fatty acid desaturase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015876Acyl-CoA desaturasePRINTSPR00075FACDDSATRASEcoord: 58..80
score: 19.68
coord: 81..101
score: 33.08
coord: 118..147
score: 47.89
IPR015876Acyl-CoA desaturasePANTHERPTHR11351ACYL-COA DESATURASEcoord: 32..258
IPR015876Acyl-CoA desaturaseCDDcd03505Delta9-FADS-likecoord: 58..258
e-value: 3.85915E-39
score: 132.294
IPR005804Fatty acid desaturase domainPFAMPF00487FA_desaturasecoord: 59..247
e-value: 2.4E-14
score: 53.8
NoneNo IPR availablePANTHERPTHR11351:SF93LIPID DESATURASE ADS3.2, CHLOROPLASTIC-RELATEDcoord: 32..258

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg014393.1Spg014393.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006633 fatty acid biosynthetic process
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016717 oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water