Spg014356 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg014356
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Pectin lyase-like superfamily protein
Location	scaffold3: 47531916 .. 47536040 (-)
RNA-Seq Expression	Spg014356
Synteny	Spg014356

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGTAGTATATAATGGATTGGCGCTTTGGGTATTAAAGATATTTCAAACTACAATTGGATTAGCCGTAAAAGCCTACAAATTCCGCGAAAGCACTTTTTACCATCGCTTGAAAGCTCTGTCTCTGAACATGAAGGGCCAACGATTGTGGCCCTCTCCAATACCCATCTTCGTATTTACTCTGTTGTCGAGCTTAATCTTTGAATCTTCAGCTGAATTTGACTCGTTATTGCAGCTTCCACGTTCTGGGTCGAACACAACTCGACCCAGATCCAAAAGGGTTCTCTATCTAGGCGATTTTGGGGCAAAAGGAGATGGGATTAGCAATGATACTCAGGTATTGTAATGAATGCCGAGATTGTATTCTTTTCGAGCTCTTTATGGACGTTGACTATTTATTGATGAAGATGGTGTTTTTCAGTTTCTTGAATTTCCTGCTATGAGTTAGAACGATTGCTTCATTGCTAAGATTGGAGTGGTGGAAGAATAATAACTTTCTTTGCAATTTTTTCATTGATTTTCTATCTTGATTCTTTGTAGCCTTTTGAATTTTTGGTGCGTTGTTCTCCTTTTTAAGCTCGATTAGACAGACATTTGACATTATGAAGTGGAATGTTTGTTTTTGCTAATGTTGTAGTGTTTTAGCTCTAGGCACTCTTGGCTGCCTGGAAGATGGCTTGTTCTTTCCCCACCAGAACACAAATTGTTTTTATAGCTAGTAACACATTTCTGGTGCACCCTATTGAGCTTCATGGTCCTTGCAGTGATTGTATATTTTCAAGCTTTTATGAACATTGACTATCTGATGATGATGATGTTTTCCCTTTCCTCGATGTTCCTGCTATGAGTTAGAATGATTGCTTCATTGCTTAGGTTGGAATGCTAGAAGTATAAGAACTTTCTCTACAACTTTCTCTTTATTTTCCATCTTGATTCTTTGTAGCATTTGAATGTTTGCTGTCTTCTTCTCCTTTTTAAGCTCGAGTCGACAGACATCTGTGACATTATGAAGTAGAAAATTCGTTTTAGTTGATGTAATAATTTTGGCTCTAGGCATCCTTTTTAAGCTTGATTAGGCAGACATCTGTGCCATTATGAAGTAGAATGTTCATTTTTGCTCATGTAGTGATAATTGTGGCTCTAGGCATTACTGGCTGCCTGGAAGGTGGCTTGTTCTTTCCCTACTAGAGCACACATTTTTTTTTCCGGCTGGTAACACATTTCTGGTGCACCCTATTGAGTTTTATGGTCCTTGCAGATATTGTATATTTTCAAGCTTTTTATGAACGTCGATTATCTGATGATGATGGTGCTGGTTTCCCTTTTCTTGATTTTCCTGTTATGGGTTAGAAGCTTAGGTTTAAATGAGAACTTTCTCTGCCACTTTCTCACGATTTTCCATCTTGATTTTTCTGTAGCCTTTTGAATGTTTGGTGCCTTCTTTGCCTTTTTAAGCTCAATTAGTCAGATGTTTGTAACATTATGAAGTAGAATGTTCGTTTTTGCTAATGTTGTATTGTAATAATTTTTGGCTCAAGGCATTCATGGAAGCCTGGAAGATGGCTTGTTCTTTCCCCACTAGAATACGAATTGTTTTTCCAGCTAGTGACACATTTCTGGTGCACCCTATTGAGCTTCATGGTCCTTGTCGGTCAAGGGTGACCTTGAGGGTATGAGCCAAATGCCATTCTTTTCCCCTTCGAACAGTACCTCTTTGCCTCTTATATGCTTTTAGATTTTTTATCCCTCAATTATTTCCTTACATTCAGTGTTGTAGTTTCTATTTTAAAAGATTCATGGCACAATCAATGCTCCCAAAGATCCAAATGCCTGGGCTGGTCGAAATCCACGGAAATGGCTTTACTTCCGTGGAGTGAACCACCTGACTCTTGAAGGCGGAGGAGAGATTAACGGAATGGGATATGAATGGTGGATGACATCTTGCAAGATTAACTCAACAAATGTATTTTCTTCTTTGATCCCTGTATCCTTCTGTCTCATTTCAAGATAACTTGCGTAAATCAGTCTCAAAGTAGGCAATAACCTTCATTTTTTCCATCTTTTGTATGGCAGCCGTGTCGACATGCTCCAACAGTGAGTGGTTCTTGTGCTATTTGTGCTTTGGTTTTTCAGCTGAGTTTTGACATGCATTGAAAATAAATTCTGCGTACAGTGACATTTTCACAATTCTATTGCAGGCTCTTACTTTCCATAAGTGCAAGAATTTAAAGATTCATCACCTATTGGTAATTGACAGTCAACAGATGCATGTTTCTTTCACAAGTTGTCTCCGCGTCGTAGCATCCAATCTCAAAGTGATCGCTCCTGGTTTTAGCCCAAATACCGATGGAATCCATATCAGCGCATCAAAGGGTGTCACAGTCAAGAATAGCATCATTAGTACAGGTCAGTCATCTCATCATTTAACAGATTATTCTATCTTCTGCTGAGTTTACATTTATGAAAGACATAATAATCAACCTTCAGATGTTTTCATAGTTGTTAAACTTGAATCCATAGTTGAATGAGAACTTTTTCTTAAGGCATGTTTGGAAGAGCACGAGACACAAGAAAGTCCTAGAGAACAAAGAAGATTTTCAGCCGAAAACTTCCAATTGCTGTGACCAAATGCAACAAATTTTTGTCAATTTAATTCAGTGACTGACATTCAAGGGCAATATATATGCAACTCAAGTAGGCTTTGTATTGCTCTCATAACGTTTGGCTTAGTTTGATGCCCGAATTTTCCCCAGCCATCTGATTTTGTGCACCAAGTTTTCTTTTTGCAAATTAGTCTGCAGATGAATGGGTTGATATCATATCAATTTTGGACCCTAATAGTTAAGATTCAATTTGTGGATGTGAGCTGAGTTGACAACATTCTCAAGTTCATTTTGGTATCTTTCAGCCCAATTCGACTCTCTAAATTGAAAATCTGACAAGAAAGATGATGATCAAACAGTATCCTTTGCTGTTATGTAATAGAGAATTCTAATGCAGATGCACTCTCTTTGCAGGGGATGACTGTATCTCCATTGTCAGTAATTCTTCAAGGGTCTTGATCAGAGACATTGTCTGTGGGCCAGGCCATGGAATAAGGTAACAAAACTCTTCGCTAGTTAGATTACTCTGATTGGATCAAAGAAGTTGGTGTGGATAAATCAATTTTTTGTTCATCAGTATTGGAAGCTTGGGAAAGTACAACACATGGGGGCAGGTACATAATGTAAGGGTCGATGGAGCTGTTTTGTCGAACACCGAAAATGGAGCAAGGATCAAAACTTGGCAGGTAAAATGTGTTTGTTCATGCTCGTTTCATATATACATTGTCTCCCACAAACTATTTTAGCTGATCTGTGCTCCTGTTTCATATATCCATCAGGGGGGCAATGGCTCTGCCACTGATATTACCTTCAAGAACATGTTGATGAAAAATGTATCAAATCCAATAATCATTGATCAGTATTACTGTGACTCGGATACACCATGTGCCAATCAGGTAAGGGTTCCAACTCATTCATTTCCGAACGCGAAAGACGTACCTGGCTTTTCCATTTCATTGACCCTTCAATCAAAATTACTACAACTTCTTGTTAATGAGATTGCAGACATCAGCTATCAAAGTGGAAGACATATCATTCATTCACATCAAAGGAACTACAGCAACAAAGGAAGCCATAAAATTTGCTTGCAGCGATAAGTCGCCATGTGAAGGCTTGTTTCTGGAAGACATTCTTCTATTATCGAACGATGGCGAGATGTCAACTTCTTTCTGTTGGCAAGCACATGGTTCGAATTCAGGGCCTGTTTATCCACCGCCGTGCTTTCTCGGTAACGAAAACTTCATTGAGCAGAAGGTCCAGCCTTACCCTGCTTCTCATTCTTTTTAGACACAACTTCTGAAGCTTTATAGTTGCATAAGCTTATGAGAGGTTCATTACCTATGCTACATATTGTATCTTTTCCTATCTCTGTTTTACTCTGCCTCCATTTCCCTGTTTATTTGCCTCTCAACAGATGAGGCTGTGATGTTATATTGTATGTATGAATGTATGAACTGTAGAAACTGAAAGAAAATTTCAATAAGACAGACTGAAATCCTAGCTTTAATTAATTAATATATTATAG

mRNA sequence

ATGAAGGGCCAACGATTGTGGCCCTCTCCAATACCCATCTTCGTATTTACTCTGTTGTCGAGCTTAATCTTTGAATCTTCAGCTGAATTTGACTCGTTATTGCAGCTTCCACGTTCTGGGTCGAACACAACTCGACCCAGATCCAAAAGGGTTCTCTATCTAGGCGATTTTGGGGCAAAAGGAGATGGGATTAGCAATGATACTCAGGCACTCTTGGCTGCCTGGAAGATGGCTTGTTCTTTCCCCACCAGAACACAAATTGTTTTTATAGCTAGTAACACATTTCTGGTGCACCCTATTGAGCTTCATGGTCCTTGCAGTGATTATATTGTATATTTTCAAGCTTTTTATGAACGTCGATTATCTGATGATGATGGTGCTGGTTTCCCTTTTCTTGATTTTCCTGCATTCATGGAAGCCTGGAAGATGGCTTGTTCTTTCCCCACTAGAATACGAATTGTTTTTCCAGCTAGTGACACATTTCTGGTGCACCCTATTGAGCTTCATGGTCCTTGTCGGTCAAGGGTGACCTTGAGGATTCATGGCACAATCAATGCTCCCAAAGATCCAAATGCCTGGGCTGGTCGAAATCCACGGAAATGGCTTTACTTCCGTGGAGTGAACCACCTGACTCTTGAAGGCGGAGGAGAGATTAACGGAATGGGATATGAATGGTGGATGACATCTTGCAAGATTAACTCAACAAATCCGTGTCGACATGCTCCAACAGCTCTTACTTTCCATAAGTGCAAGAATTTAAAGATTCATCACCTATTGGTAATTGACAGTCAACAGATGCATGTTTCTTTCACAAGTTGTCTCCGCGTCGTAGCATCCAATCTCAAAGTGATCGCTCCTGGTTTTAGCCCAAATACCGATGGAATCCATATCAGCGCATCAAAGGGTGTCACAGTCAAGAATAGCATCATTAGTACAGGGGATGACTGTATCTCCATTGTCAGTAATTCTTCAAGGGTCTTGATCAGAGACATTGTCTGTGGGCCAGGCCATGGAATAAGTATTGGAAGCTTGGGAAAGTACAACACATGGGGGCAGGTACATAATGTAAGGGTCGATGGAGCTGTTTTGTCGAACACCGAAAATGGAGCAAGGATCAAAACTTGGCAGGGGGGCAATGGCTCTGCCACTGATATTACCTTCAAGAACATGTTGATGAAAAATGTATCAAATCCAATAATCATTGATCAGTATTACTGTGACTCGGATACACCATGTGCCAATCAGACATCAGCTATCAAAGTGGAAGACATATCATTCATTCACATCAAAGGAACTACAGCAACAAAGGAAGCCATAAAATTTGCTTGCAGCGATAAGTCGCCATGTGAAGGCTTGTTTCTGGAAGACATTCTTCTATTATCGAACGATGGCGAGATGTCAACTTCTTTCTGTTGGCAAGCACATGGTTCGAATTCAGGGCCTGTTTATCCACCGCCGTGCTTTCTCGGTAACGAAAACTTCATTGAGCAGAAGGTCCAGCCTTACCCTGCTTCTCATTCTTTTTAG

Coding sequence (CDS)

ATGAAGGGCCAACGATTGTGGCCCTCTCCAATACCCATCTTCGTATTTACTCTGTTGTCGAGCTTAATCTTTGAATCTTCAGCTGAATTTGACTCGTTATTGCAGCTTCCACGTTCTGGGTCGAACACAACTCGACCCAGATCCAAAAGGGTTCTCTATCTAGGCGATTTTGGGGCAAAAGGAGATGGGATTAGCAATGATACTCAGGCACTCTTGGCTGCCTGGAAGATGGCTTGTTCTTTCCCCACCAGAACACAAATTGTTTTTATAGCTAGTAACACATTTCTGGTGCACCCTATTGAGCTTCATGGTCCTTGCAGTGATTATATTGTATATTTTCAAGCTTTTTATGAACGTCGATTATCTGATGATGATGGTGCTGGTTTCCCTTTTCTTGATTTTCCTGCATTCATGGAAGCCTGGAAGATGGCTTGTTCTTTCCCCACTAGAATACGAATTGTTTTTCCAGCTAGTGACACATTTCTGGTGCACCCTATTGAGCTTCATGGTCCTTGTCGGTCAAGGGTGACCTTGAGGATTCATGGCACAATCAATGCTCCCAAAGATCCAAATGCCTGGGCTGGTCGAAATCCACGGAAATGGCTTTACTTCCGTGGAGTGAACCACCTGACTCTTGAAGGCGGAGGAGAGATTAACGGAATGGGATATGAATGGTGGATGACATCTTGCAAGATTAACTCAACAAATCCGTGTCGACATGCTCCAACAGCTCTTACTTTCCATAAGTGCAAGAATTTAAAGATTCATCACCTATTGGTAATTGACAGTCAACAGATGCATGTTTCTTTCACAAGTTGTCTCCGCGTCGTAGCATCCAATCTCAAAGTGATCGCTCCTGGTTTTAGCCCAAATACCGATGGAATCCATATCAGCGCATCAAAGGGTGTCACAGTCAAGAATAGCATCATTAGTACAGGGGATGACTGTATCTCCATTGTCAGTAATTCTTCAAGGGTCTTGATCAGAGACATTGTCTGTGGGCCAGGCCATGGAATAAGTATTGGAAGCTTGGGAAAGTACAACACATGGGGGCAGGTACATAATGTAAGGGTCGATGGAGCTGTTTTGTCGAACACCGAAAATGGAGCAAGGATCAAAACTTGGCAGGGGGGCAATGGCTCTGCCACTGATATTACCTTCAAGAACATGTTGATGAAAAATGTATCAAATCCAATAATCATTGATCAGTATTACTGTGACTCGGATACACCATGTGCCAATCAGACATCAGCTATCAAAGTGGAAGACATATCATTCATTCACATCAAAGGAACTACAGCAACAAAGGAAGCCATAAAATTTGCTTGCAGCGATAAGTCGCCATGTGAAGGCTTGTTTCTGGAAGACATTCTTCTATTATCGAACGATGGCGAGATGTCAACTTCTTTCTGTTGGCAAGCACATGGTTCGAATTCAGGGCCTGTTTATCCACCGCCGTGCTTTCTCGGTAACGAAAACTTCATTGAGCAGAAGGTCCAGCCTTACCCTGCTTCTCATTCTTTTTAG

Protein sequence

MKGQRLWPSPIPIFVFTLLSSLIFESSAEFDSLLQLPRSGSNTTRPRSKRVLYLGDFGAKGDGISNDTQALLAAWKMACSFPTRTQIVFIASNTFLVHPIELHGPCSDYIVYFQAFYERRLSDDDGAGFPFLDFPAFMEAWKMACSFPTRIRIVFPASDTFLVHPIELHGPCRSRVTLRIHGTINAPKDPNAWAGRNPRKWLYFRGVNHLTLEGGGEINGMGYEWWMTSCKINSTNPCRHAPTALTFHKCKNLKIHHLLVIDSQQMHVSFTSCLRVVASNLKVIAPGFSPNTDGIHISASKGVTVKNSIISTGDDCISIVSNSSRVLIRDIVCGPGHGISIGSLGKYNTWGQVHNVRVDGAVLSNTENGARIKTWQGGNGSATDITFKNMLMKNVSNPIIIDQYYCDSDTPCANQTSAIKVEDISFIHIKGTTATKEAIKFACSDKSPCEGLFLEDILLLSNDGEMSTSFCWQAHGSNSGPVYPPPCFLGNENFIEQKVQPYPASHSF

Homology

[Close]

BLAST of Spg014356 vs. NCBI nr
Match: XP_023547734.1 (probable polygalacturonase At1g80170 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 797.7 bits (2059), Expect = 5.7e-227
Identity = 389/508 (76.57%), Postives = 412/508 (81.10%), Query Frame = 0


Query: 1   MKGQRLWPSPIPIFVFTLLSSLIFESSAEFDSLLQLPRSGSNTTRPRSKRVLYLGDFGAK 60
           MK  R W SPIPIFVFT+L+SL FE SAEFDSLLQLP SG+N +RPRSKRV YL DFGAK
Sbjct: 1   MKAHRPWRSPIPIFVFTVLASLSFECSAEFDSLLQLPLSGANRSRPRSKRVFYLADFGAK 60

Query: 61  GDGISNDTQALLAAWKMACSFPTRTQIVFIASNTFLVHPIELHGPCSDYIVYFQAFYERR 120
           GDGISNDTQ L+                                                
Sbjct: 61  GDGISNDTQPLI------------------------------------------------ 120

Query: 121 LSDDDGAGFPFLDFPAFMEAWKMACSFPTRIRIVFPASDTFLVHPIELHGPCRSRVTLRI 180
                             EAWK+ACSFPTR RIVFPAS+TFLVHPIELHGPCRSRVTLRI
Sbjct: 121 ------------------EAWKIACSFPTRTRIVFPASNTFLVHPIELHGPCRSRVTLRI 180

Query: 181 HGTINAPKDPNAWAGRNPRKWLYFRGVNHLTLEGGGEINGMGYEWWMTSCKINSTNPCRH 240
           HGTINAPKDPN W GRNPRKWLYFR VNHLTLEGGGEINGMGY+WWM SCKINSTNPCRH
Sbjct: 181 HGTINAPKDPNVWEGRNPRKWLYFRAVNHLTLEGGGEINGMGYKWWMRSCKINSTNPCRH 240

Query: 241 APTALTFHKCKNLKIHHLLVIDSQQMHVSFTSCLRVVASNLKVIAPGFSPNTDGIHISAS 300
           APTALTFHKCKNLKIHHLLVIDSQQMHVSFT+CLRVVASNLKVIAP FSPNTDGIHISAS
Sbjct: 241 APTALTFHKCKNLKIHHLLVIDSQQMHVSFTNCLRVVASNLKVIAPAFSPNTDGIHISAS 300

Query: 301 KGVTVKNSIISTGDDCISIVSNSSRVLIRDIVCGPGHGISIGSLGKYNTWGQVHNVRVDG 360
           KGVTVKNS++STGDDCISIV+NSSR+LI+DI CGPGHGISIGSLGK NTW QVHN+RVDG
Sbjct: 301 KGVTVKNSVVSTGDDCISIVNNSSRILIKDIACGPGHGISIGSLGKQNTWAQVHNIRVDG 360

Query: 361 AVLSNTENGARIKTWQGGNGSATDITFKNMLMKNVSNPIIIDQYYCDSDTPCANQTSAIK 420
           AVLSNTENGARIKTWQGG+G ATDITFKNMLMKNVSNPIIIDQYYCDS+ PCANQTSA+K
Sbjct: 361 AVLSNTENGARIKTWQGGSGFATDITFKNMLMKNVSNPIIIDQYYCDSNHPCANQTSAVK 420

Query: 421 VEDISFIHIKGTTATKEAIKFACSDKSPCEGLFLEDILLLSNDGEMSTSFCWQAHGSNSG 480
           V DISFIHIKGTTAT EAIKFACSD  PC  L++ED+LLLSNDGEM TSFCWQAHGS+SG
Sbjct: 421 VGDISFIHIKGTTATNEAIKFACSDTLPCVDLYMEDVLLLSNDGEMLTSFCWQAHGSSSG 442

Query: 481 PVYPPPCFLGNENFIEQKVQPYPASHSF 509
           PVYPPPCFLGNE+FIEQKVQP PASHSF
Sbjct: 481 PVYPPPCFLGNESFIEQKVQPRPASHSF 442

[Close]

BLAST of Spg014356 vs. NCBI nr
Match: XP_038897077.1 (probable polygalacturonase At1g80170 [Benincasa hispida])

HSP 1 Score: 797.3 bits (2058), Expect = 7.4e-227
Identity = 395/508 (77.76%), Postives = 412/508 (81.10%), Query Frame = 0


Query: 1   MKGQRLWPSPIPIFVFTLLSSLIFESSAEFDSLLQLPRSGSNTTRPRSKRVLYLGDFGAK 60
           MKGQRLWPSPIPIF+FT+L+SL FE SAEFDSLLQLP SG NTTRPRSK V YLGDFGAK
Sbjct: 1   MKGQRLWPSPIPIFLFTVLTSLSFECSAEFDSLLQLPLSGFNTTRPRSKGVFYLGDFGAK 60

Query: 61  GDGISNDTQALLAAWKMACSFPTRTQIVFIASNTFLVHPIELHGPCSDYIVYFQAFYERR 120
           GDGISNDTQAL                                                 
Sbjct: 61  GDGISNDTQAL------------------------------------------------- 120

Query: 121 LSDDDGAGFPFLDFPAFMEAWKMACSFPTRIRIVFPASDTFLVHPIELHGPCRSRVTLRI 180
                            MEAWKMAC FPTR RIVFPAS+TFLVHPIELHGPCRSRVTLRI
Sbjct: 121 -----------------MEAWKMACFFPTRARIVFPASNTFLVHPIELHGPCRSRVTLRI 180

Query: 181 HGTINAPKDPNAWAGRNPRKWLYFRGVNHLTLEGGGEINGMGYEWWMTSCKINSTNPCRH 240
           +GTINAPKDPNAWAG NPRKWLYFRGVNHLTLEGGGEINGMGYEWWM SCKINSTN CRH
Sbjct: 181 YGTINAPKDPNAWAGLNPRKWLYFRGVNHLTLEGGGEINGMGYEWWMRSCKINSTNLCRH 240

Query: 241 APTALTFHKCKNLKIHHLLVIDSQQMHVSFTSCLRVVASNLKVIAPGFSPNTDGIHISAS 300
           APTA+TFHKCKNLK+HHLL+IDSQQMHVSFT+CLRVVASNLKVIAP FSPNTDGIHISAS
Sbjct: 241 APTAITFHKCKNLKVHHLLIIDSQQMHVSFTNCLRVVASNLKVIAPAFSPNTDGIHISAS 300

Query: 301 KGVTVKNSIISTGDDCISIVSNSSRVLIRDIVCGPGHGISIGSLGKYNTWGQVHNVRVDG 360
           KGV VKNSIISTGDDC+SIVSNSSRVLIRDIVCGPGHGISIGSLGK NT  QV NVRVDG
Sbjct: 301 KGVMVKNSIISTGDDCVSIVSNSSRVLIRDIVCGPGHGISIGSLGKQNTTAQVRNVRVDG 360

Query: 361 AVLSNTENGARIKTWQGGNGSATDITFKNMLMKNVSNPIIIDQYYCDSDTPCANQTSAIK 420
           AVLSNTENGARIKTWQGG+G  TD+TFKNMLMKNVSNPIIIDQYYCDS+ PCANQTSA+K
Sbjct: 361 AVLSNTENGARIKTWQGGSGCVTDVTFKNMLMKNVSNPIIIDQYYCDSNLPCANQTSAVK 420

Query: 421 VEDISFIHIKGTTATKEAIKFACSDKSPCEGLFLEDILLLSNDGEMSTSFCWQAHGSNSG 480
           +EDISFIHIKGTTAT EAIKFACSD  PC GLFLEDILLLSN  E+STS CWQAHGS SG
Sbjct: 421 IEDISFIHIKGTTATTEAIKFACSDTLPCVGLFLEDILLLSNISEISTSSCWQAHGSISG 442

Query: 481 PVYPPPCFLGNENFIEQKVQPYPASHSF 509
           PVYPPPCFL NENFI+QKVQP PASHSF
Sbjct: 481 PVYPPPCFLSNENFIQQKVQPDPASHSF 442

[Close]

BLAST of Spg014356 vs. NCBI nr
Match: XP_022991643.1 (probable polygalacturonase At1g80170 [Cucurbita maxima])

HSP 1 Score: 788.5 bits (2035), Expect = 3.5e-224
Identity = 384/508 (75.59%), Postives = 408/508 (80.31%), Query Frame = 0


Query: 1   MKGQRLWPSPIPIFVFTLLSSLIFESSAEFDSLLQLPRSGSNTTRPRSKRVLYLGDFGAK 60
           MK  RLW SPIPIFVFT+L+ L FE SAEFDSLLQLP SG+N +R RSKRV YL DFGAK
Sbjct: 1   MKAHRLWRSPIPIFVFTVLARLSFECSAEFDSLLQLPLSGANRSRARSKRVFYLADFGAK 60

Query: 61  GDGISNDTQALLAAWKMACSFPTRTQIVFIASNTFLVHPIELHGPCSDYIVYFQAFYERR 120
           GDGISNDTQ L+                                                
Sbjct: 61  GDGISNDTQPLI------------------------------------------------ 120

Query: 121 LSDDDGAGFPFLDFPAFMEAWKMACSFPTRIRIVFPASDTFLVHPIELHGPCRSRVTLRI 180
                             EAWK+ACSFPTR RIVFPAS+TFLVHPIELHGPCRSRVTLRI
Sbjct: 121 ------------------EAWKIACSFPTRTRIVFPASNTFLVHPIELHGPCRSRVTLRI 180

Query: 181 HGTINAPKDPNAWAGRNPRKWLYFRGVNHLTLEGGGEINGMGYEWWMTSCKINSTNPCRH 240
           HGTINAPKDPN W  RNPRKWLYFR VNHLTL GGGEINGMGYEWWM SCKINSTNPCRH
Sbjct: 181 HGTINAPKDPNVWEHRNPRKWLYFRAVNHLTLNGGGEINGMGYEWWMRSCKINSTNPCRH 240

Query: 241 APTALTFHKCKNLKIHHLLVIDSQQMHVSFTSCLRVVASNLKVIAPGFSPNTDGIHISAS 300
           APTA+TFHKCKN+KIHHLLVIDSQQMHVSFT+CLRVVASNLKVIAP FSPNTDGIHISAS
Sbjct: 241 APTAITFHKCKNVKIHHLLVIDSQQMHVSFTNCLRVVASNLKVIAPAFSPNTDGIHISAS 300

Query: 301 KGVTVKNSIISTGDDCISIVSNSSRVLIRDIVCGPGHGISIGSLGKYNTWGQVHNVRVDG 360
           KGVTVKNS++STGDDCISIV+NSSR+LI+DI CGPGHGISIGSLGK NTW QVHN+RVDG
Sbjct: 301 KGVTVKNSVVSTGDDCISIVNNSSRILIKDIACGPGHGISIGSLGKQNTWAQVHNIRVDG 360

Query: 361 AVLSNTENGARIKTWQGGNGSATDITFKNMLMKNVSNPIIIDQYYCDSDTPCANQTSAIK 420
           AVLSNTENGARIKTWQGG+G ATDITFKNMLMKNVSNPIIIDQYYCDS+ PCANQTSA+K
Sbjct: 361 AVLSNTENGARIKTWQGGSGFATDITFKNMLMKNVSNPIIIDQYYCDSNHPCANQTSAVK 420

Query: 421 VEDISFIHIKGTTATKEAIKFACSDKSPCEGLFLEDILLLSNDGEMSTSFCWQAHGSNSG 480
           V DISFIHIKGTTAT EAIK ACSD  PC  L++ED+LLLSNDGEM TSFCWQAHGS+SG
Sbjct: 421 VRDISFIHIKGTTATNEAIKLACSDTLPCVDLYMEDVLLLSNDGEMLTSFCWQAHGSSSG 442

Query: 481 PVYPPPCFLGNENFIEQKVQPYPASHSF 509
           PVYPPPCFLGNE+FIEQKVQP PASHSF
Sbjct: 481 PVYPPPCFLGNESFIEQKVQPRPASHSF 442

[Close]

BLAST of Spg014356 vs. NCBI nr
Match: KAG7014128.1 (putative polygalacturonase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 785.0 bits (2026), Expect = 3.8e-223
Identity = 382/508 (75.20%), Postives = 407/508 (80.12%), Query Frame = 0


Query: 1   MKGQRLWPSPIPIFVFTLLSSLIFESSAEFDSLLQLPRSGSNTTRPRSKRVLYLGDFGAK 60
           M+  R W SPIPI VFT+L+ L FE SAEFDSLLQLP SG+N +R RSKRV YL DFGAK
Sbjct: 1   MEAHRFWRSPIPILVFTVLARLSFECSAEFDSLLQLPLSGANRSRSRSKRVFYLADFGAK 60

Query: 61  GDGISNDTQALLAAWKMACSFPTRTQIVFIASNTFLVHPIELHGPCSDYIVYFQAFYERR 120
           GDGISNDTQ L+                                                
Sbjct: 61  GDGISNDTQPLI------------------------------------------------ 120

Query: 121 LSDDDGAGFPFLDFPAFMEAWKMACSFPTRIRIVFPASDTFLVHPIELHGPCRSRVTLRI 180
                             EAWK ACSFPTR RIVFPAS+TFLVHPIELHGPCRSRVTLRI
Sbjct: 121 ------------------EAWKTACSFPTRTRIVFPASNTFLVHPIELHGPCRSRVTLRI 180

Query: 181 HGTINAPKDPNAWAGRNPRKWLYFRGVNHLTLEGGGEINGMGYEWWMTSCKINSTNPCRH 240
           HGTINAPKDPN W  RNPRKWLYFR VNHLTL GGGEINGMGY+WWM SCKINSTNPCRH
Sbjct: 181 HGTINAPKDPNVWERRNPRKWLYFRAVNHLTLNGGGEINGMGYKWWMRSCKINSTNPCRH 240

Query: 241 APTALTFHKCKNLKIHHLLVIDSQQMHVSFTSCLRVVASNLKVIAPGFSPNTDGIHISAS 300
           APTALTFHKCKNLKIHHLLVIDSQQMHVSFT+CLRVVASNLKVIAP FSPNTDGIHISAS
Sbjct: 241 APTALTFHKCKNLKIHHLLVIDSQQMHVSFTNCLRVVASNLKVIAPAFSPNTDGIHISAS 300

Query: 301 KGVTVKNSIISTGDDCISIVSNSSRVLIRDIVCGPGHGISIGSLGKYNTWGQVHNVRVDG 360
           KGVTVKNS++STGDDCISIV+NSSR+LI+DI CGPGHGISIGSLGK NTW QVHN+RVDG
Sbjct: 301 KGVTVKNSVVSTGDDCISIVNNSSRILIKDIACGPGHGISIGSLGKQNTWAQVHNIRVDG 360

Query: 361 AVLSNTENGARIKTWQGGNGSATDITFKNMLMKNVSNPIIIDQYYCDSDTPCANQTSAIK 420
           AVLSNTENGARIKTWQGG+G ATDITFKNMLMKNVSNPIIIDQYYCDS+ PCANQTSA++
Sbjct: 361 AVLSNTENGARIKTWQGGSGFATDITFKNMLMKNVSNPIIIDQYYCDSNHPCANQTSAVE 420

Query: 421 VEDISFIHIKGTTATKEAIKFACSDKSPCEGLFLEDILLLSNDGEMSTSFCWQAHGSNSG 480
           V DISFIHIKGTTAT EAIKFACSD  PC  L++ED+LLLSNDGEM TSFCWQAHGS+SG
Sbjct: 421 VRDISFIHIKGTTATNEAIKFACSDTLPCVDLYMEDVLLLSNDGEMLTSFCWQAHGSSSG 442

Query: 481 PVYPPPCFLGNENFIEQKVQPYPASHSF 509
           PVYPPPCFLGNE+FIEQKVQP+PASHSF
Sbjct: 481 PVYPPPCFLGNESFIEQKVQPHPASHSF 442

[Close]

BLAST of Spg014356 vs. NCBI nr
Match: KAG6575587.1 (putative polygalacturonase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 783.9 bits (2023), Expect = 8.5e-223
Identity = 382/508 (75.20%), Postives = 407/508 (80.12%), Query Frame = 0


Query: 1   MKGQRLWPSPIPIFVFTLLSSLIFESSAEFDSLLQLPRSGSNTTRPRSKRVLYLGDFGAK 60
           M+  R W SPIPI VFT+L+ L FE SAEFDSLLQLP SG+N +R RSKRV YL DFGAK
Sbjct: 1   MEAHRFWRSPIPILVFTVLARLSFECSAEFDSLLQLPLSGANRSRSRSKRVFYLADFGAK 60

Query: 61  GDGISNDTQALLAAWKMACSFPTRTQIVFIASNTFLVHPIELHGPCSDYIVYFQAFYERR 120
           GDGISNDTQ L+                                                
Sbjct: 61  GDGISNDTQPLI------------------------------------------------ 120

Query: 121 LSDDDGAGFPFLDFPAFMEAWKMACSFPTRIRIVFPASDTFLVHPIELHGPCRSRVTLRI 180
                             EAWK ACSFPTR RIVFPAS+TFLVHPIELHGPCRSRVTLRI
Sbjct: 121 ------------------EAWKTACSFPTRTRIVFPASNTFLVHPIELHGPCRSRVTLRI 180

Query: 181 HGTINAPKDPNAWAGRNPRKWLYFRGVNHLTLEGGGEINGMGYEWWMTSCKINSTNPCRH 240
           HGTINAPKDPN W  RNPRKWLYFR VNHLTL GGGEINGMGY+WWM SCKINSTNPCRH
Sbjct: 181 HGTINAPKDPNVWERRNPRKWLYFRAVNHLTLNGGGEINGMGYKWWMRSCKINSTNPCRH 240

Query: 241 APTALTFHKCKNLKIHHLLVIDSQQMHVSFTSCLRVVASNLKVIAPGFSPNTDGIHISAS 300
           APTALTFHKCKNLKIHHLLVIDSQQMHVSFT+CLRVVASNLKVIAP FSPNTDGIHISAS
Sbjct: 241 APTALTFHKCKNLKIHHLLVIDSQQMHVSFTNCLRVVASNLKVIAPAFSPNTDGIHISAS 300

Query: 301 KGVTVKNSIISTGDDCISIVSNSSRVLIRDIVCGPGHGISIGSLGKYNTWGQVHNVRVDG 360
           KGVTVKNS++STGDDCISIV+NSSR+LI+DI CGPGHGISIGSLGK NTW QVHN+RVDG
Sbjct: 301 KGVTVKNSVVSTGDDCISIVNNSSRILIKDIACGPGHGISIGSLGKQNTWAQVHNIRVDG 360

Query: 361 AVLSNTENGARIKTWQGGNGSATDITFKNMLMKNVSNPIIIDQYYCDSDTPCANQTSAIK 420
           AVLSNTENGARIKTWQGG+G ATDITFKNMLMKNVSNPIIIDQYYCDS+ PCANQTSA+K
Sbjct: 361 AVLSNTENGARIKTWQGGSGFATDITFKNMLMKNVSNPIIIDQYYCDSNHPCANQTSAVK 420

Query: 421 VEDISFIHIKGTTATKEAIKFACSDKSPCEGLFLEDILLLSNDGEMSTSFCWQAHGSNSG 480
           V DISFIHIKGTTAT EAIKFACSD  PC  L++ED+LLLSN+GEM TSFCWQAHGS+SG
Sbjct: 421 VGDISFIHIKGTTATNEAIKFACSDTLPCVDLYMEDVLLLSNNGEMLTSFCWQAHGSSSG 442

Query: 481 PVYPPPCFLGNENFIEQKVQPYPASHSF 509
           PVYPPPCFLGNE+FIEQKVQP+PASHSF
Sbjct: 481 PVYPPPCFLGNESFIEQKVQPHPASHSF 442

[Close]

BLAST of Spg014356 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 1.4e-148
Identity = 254/490 (51.84%), Postives = 334/490 (68.16%), Query Frame = 0


Query: 13  IFVFTLLSSLIFESSA-EFDSLLQLPRSGSNTTRPRSKRVLYLGDFGAKGDGISNDTQAL 72
           + +  + SSL   ++A  F+SLLQLPR  S +TRPRS+R+L++G+FGAKG+G+++DT+  
Sbjct: 13  LLLLVVASSLALTANANSFESLLQLPRRQSRSTRPRSERLLHVGNFGAKGNGVTDDTK-- 72

Query: 73  LAAWKMACSFPTRTQIVFIASNTFLVHPIELHGPCSDYIVYFQAFYERRLSDDDGAGFPF 132
                                                                       
Sbjct: 73  ------------------------------------------------------------ 132

Query: 133 LDFPAFMEAWKMACSFPTRIRIVFPASDTFLVHPIELHGPCRSRVTLRIHGTINAPKDPN 192
               AF +AWK ACS   + RI+ P + T L+ PI+L GPC++R+TL+I GTI AP DP+
Sbjct: 133 ----AFADAWKTACSSKVKTRILVPENYTCLLRPIDLSGPCKARLTLQISGTIIAPNDPD 192

Query: 193 AWAGRNPRKWLYFRGVNHLTLEGGGEINGMGYEWWMTSCKINSTNPCRHAPTALTFHKCK 252
            W G N RKWLYF G++ LT+EGGG +NGMG EWW  SCK N +NPCR APTALTFHKCK
Sbjct: 193 VWEGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCKHNHSNPCRGAPTALTFHKCK 252

Query: 253 NLKIHHLLVIDSQQMHVSFTSCLRVVASNLKVIAPGFSPNTDGIHISASKGVTVKNSIIS 312
           N+++ +L VIDSQQMH++ TSC RV  S LKVIAP  SPNTDGIHIS S+G+ + N+ +S
Sbjct: 253 NMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPATSPNTDGIHISVSRGIVIDNTTVS 312

Query: 313 TGDDCISIVSNSSRVLIRDIVCGPGHGISIGSLGKYNTWGQVHNVRVDGAVLSNTENGAR 372
           TGDDCISIV NS+++ I +I+CGPGHGISIGSLGK  +W +V ++ VD A++S+T NG R
Sbjct: 313 TGDDCISIVKNSTQISISNIICGPGHGISIGSLGKSKSWEEVRDITVDTAIISDTANGVR 372

Query: 373 IKTWQGGNGSATDITFKNMLMKNVSNPIIIDQYYCDSDTPCANQTSAIKVEDISFIHIKG 432
           IKTWQGG+G  + I F+N+ M NVSNPIIIDQYYCDS  PCANQTSAI +E+ISF+H++G
Sbjct: 373 IKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDSRKPCANQTSAISIENISFVHVRG 432

Query: 433 TTATKEAIKFACSDKSPCEGLFLEDILLLSNDGE-MSTSFCWQAHGSNSGPVYPPPCFLG 492
           T+A+KEAIK +CSD SPC  + L+DI L  ++G+  + SFCW+A+GS+SG VYPPPC   
Sbjct: 433 TSASKEAIKISCSDSSPCRNILLQDIDLEPSNGDGFTESFCWEAYGSSSGQVYPPPCLSD 436

Query: 493 NENFIEQKVQ 501
           + +F+EQ VQ
Sbjct: 493 DTSFLEQSVQ 436

[Close]

BLAST of Spg014356 vs. ExPASy Swiss-Prot
Match: Q02096 (Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 3.8e-101
Identity = 180/350 (51.43%), Postives = 239/350 (68.29%), Query Frame = 0


Query: 123 DDDGA-GFPFLDFPAFMEAWKMACSFPTRIRIVFPASDTFLVHPIELHGPCRSRVTLRIH 182
           DD GA G    D  AF +AWK ACS  +   ++ P +  +L+  I   GPC+S + ++I 
Sbjct: 88  DDFGARGDGTDDTKAFEKAWKDACS--SGSVLIVPENKNYLLKQITFSGPCKSDLRVKIR 147

Query: 183 GTINAPKDPNAWAGRNPRKWLYFRGVNHLTLEGGGEINGMGYEWWMTSCKINSTNPCRHA 242
           GTI A  D + W G N ++W+ F  +++LTLEGGG  NG G  WW +SCK   + PC+ A
Sbjct: 148 GTIEASSDQSDWVGHNRKRWIEFEDISNLTLEGGGTSNGNGETWWDSSCKRKKSLPCKSA 207

Query: 243 PTALTFHKCKNLKIHHLLVIDSQQMHVSFTSCLRVVASNLKVIAPGFSPNTDGIHISASK 302
           PTALTF  CKNL +  L + DSQ+MH+SF  C  V+ASNL V AP  SPNTDGIHI+ ++
Sbjct: 208 PTALTFRSCKNLIVSDLSIKDSQKMHLSFDKCQDVIASNLMVTAPEHSPNTDGIHITGTQ 267

Query: 303 GVTVKNSIISTGDDCISIVSNSSRVLIRDIVCGPGHGISIGSLGKYNTWGQVHNVRVDGA 362
            + V NS+I TGDDCISI S S  V+  +I CGPGHGISIGSLG  N+   V  V VDG 
Sbjct: 268 RIHVMNSVIGTGDDCISIESGSKMVIATNITCGPGHGISIGSLGDRNSEAHVSGVLVDGG 327

Query: 363 VLSNTENGARIKTWQGGNGSATDITFKNMLMKNVSNPIIIDQYYCDSDTPCANQTSAIKV 422
            L +T NG RIKTWQGG+GSA +I F+N++M NV+NPIIIDQYYCDS  PC  Q SA+KV
Sbjct: 328 NLFDTTNGLRIKTWQGGSGSAKNIKFQNIVMHNVTNPIIIDQYYCDSKDPCPEQESAVKV 387

Query: 423 EDISFIHIKGTTATKEAIKFACSDKSPCEGLFLEDILLLSNDGEMSTSFC 472
            ++++++I+GT+A++ A+KF CS  SPC+G  + +I L+ N G+ +T  C
Sbjct: 388 SNVAYMNIRGTSASEVAVKFDCSKSSPCQGYIVGNINLVGNGGKETTMSC 435

[Close]

BLAST of Spg014356 vs. ExPASy Swiss-Prot
Match: P35336 (Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 7.5e-97
Identity = 179/363 (49.31%), Postives = 243/363 (66.94%), Query Frame = 0


Query: 123 DDDGA-GFPFLDFPAFMEAWKMACSFPTRIRIVFPASDTFLVHPIELHGPCRSRVTLRIH 182
           DD GA G    D  AF +AWK ACS  +   ++ P  + +LV PI   GPC+S +T++I+
Sbjct: 93  DDFGAKGDGRDDTKAFEKAWKAACSSTSSAVLLVPKKN-YLVRPISFSGPCKSGLTMQIY 152

Query: 183 GTINAPKDPNAWAGRNPRKWLYFRGVNHLTLEGGGEINGMGYEWWMTSCKINSTNPCRHA 242
           GTI A  D + +  ++ R WL F  V +L +EGGG ING G  WW  SCK N   PC+ A
Sbjct: 153 GTIEASDDRSDYR-KDGRHWLVFDSVQNLRVEGGGTINGNGKIWWQNSCKTNKALPCKDA 212

Query: 243 PTALTFHKCKNLKIHHLLVIDSQQMHVSFTSCLRVVASNLKVIAPGFSPNTDGIHISASK 302
           PTALTF+K K++ + +L + ++QQ+HVSF +C+ V ASNL V AP  SPNTDGIH++ ++
Sbjct: 213 PTALTFYKSKHVIVKNLKIENAQQIHVSFDNCVNVQASNLMVTAPENSPNTDGIHVTGTQ 272

Query: 303 GVTVKNSIISTGDDCISIVSNSSRVLIRDIVCGPGHGISIGSLGKYNTWGQVHNVRVDGA 362
            + + + +I TGDDCISIV+ S +V + DI CGPGHGISIGSLG  N+   V +V V+GA
Sbjct: 273 NIHISSCVIGTGDDCISIVNGSRKVRVNDITCGPGHGISIGSLGYGNSEAHVSDVVVNGA 332

Query: 363 VLSNTENGARIKTWQGGNGSATDITFKNMLMKNVSNPIIIDQYYCDSDTPCANQTSAIKV 422
            L  T NG RIKTWQGG+GSA++I F+N+ M NV NPIIIDQ YCD D PC  Q+SA++V
Sbjct: 333 KLCGTTNGVRIKTWQGGSGSASNIKFQNVEMHNVENPIIIDQNYCDQDKPCQEQSSAVQV 392

Query: 423 EDISFIHIKGTTATKEAIKFACSDKSPCEGLFLEDILLLSNDGEMSTSFCWQAHGSNSGP 482
           +++ + +IKGT A+  AI F CS + PC+G+ LED+ L    G  + + C     S +G 
Sbjct: 393 KNVVYQNIKGTCASNVAITFDCSKRFPCQGIVLEDVDLEIEGGAAAKALCNNVELSETGV 452

Query: 483 VYP 485
           V P
Sbjct: 453 VSP 453

[Close]

BLAST of Spg014356 vs. ExPASy Swiss-Prot
Match: P05117 (Polygalacturonase-2 OS=Solanum lycopersicum OX=4081 GN=PG2 PE=1 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 7.0e-95
Identity = 171/349 (49.00%), Postives = 232/349 (66.48%), Query Frame = 0


Query: 136 AFMEAWKMACSFPTRIRIVFPASDTFLVHPIELHGPCRSRVTLRIHGTINAPKDPNAWAG 195
           AF +AW  ACS  T ++ V P +  +L+  I   GPCRS ++++I G++ A    + +  
Sbjct: 94  AFEQAWNEACSSRTPVQFVVPKNKNYLLKQITFSGPCRSSISVKIFGSLEASSKISDYKD 153

Query: 196 RNPRKWLYFRGVNHLTLEGGGEINGMGYEWWMTSCKINSTNPCRHAPTALTFHKCKNLKI 255
           R  R W+ F  V +L + GGG ING G  WW +SCKIN + PCR APTALTF  CKNLK+
Sbjct: 154 R--RLWIAFDSVQNLVVGGGGTINGNGQVWWPSSCKINKSLPCRDAPTALTFWNCKNLKV 213

Query: 256 HHLLVIDSQQMHVSFTSCLRVVASNLKVIAPGFSPNTDGIHISASKGVTVKNSIISTGDD 315
           ++L   ++QQ+H+ F SC  VVASNL + A   SPNTDG+H+S ++ + + ++II TGDD
Sbjct: 214 NNLKSKNAQQIHIKFESCTNVVASNLMINASAKSPNTDGVHVSNTQYIQISDTIIGTGDD 273

Query: 316 CISIVSNSSRVLIRDIVCGPGHGISIGSLGKYNTWGQVHNVRVDGAVLSNTENGARIKTW 375
           CISIVS S  V   +I CGPGHGISIGSLG  N+   V NV V+ A +   ENG RIKTW
Sbjct: 274 CISIVSGSQNVQATNITCGPGHGISIGSLGSGNSEAYVSNVTVNEAKIIGAENGVRIKTW 333

Query: 376 QGGNGSATDITFKNMLMKNVSNPIIIDQYYCDSDTPCANQTSAIKVEDISFIHIKGTTAT 435
           QGG+G A++I F N+ M++V  PIIIDQ YCD   PC  Q SA++V+++ + +IKGT+AT
Sbjct: 334 QGGSGQASNIKFLNVEMQDVKYPIIIDQNYCDRVEPCIQQFSAVQVKNVVYENIKGTSAT 393

Query: 436 KEAIKFACSDKSPCEGLFLEDILLLSNDGEMSTSFCWQAHGSNSGPVYP 485
           K AIKF CS   PCEG+ +E+I L+   G+ S + C   H +N+  V P
Sbjct: 394 KVAIKFDCSTNFPCEGIIMENINLVGESGKPSEATCKNVHFNNAEHVTP 440

[Close]

BLAST of Spg014356 vs. ExPASy Swiss-Prot
Match: P48978 (Polygalacturonase OS=Malus domestica OX=3750 PE=2 SV=1)

HSP 1 Score: 330.9 bits (847), Expect = 2.6e-89
Identity = 171/346 (49.42%), Postives = 234/346 (67.63%), Query Frame = 0


Query: 123 DDDGA-GFPFLDFPAFMEAWKMACSFPTRIRIVFPASDTFLVHPIELHGPCRSRVTLRIH 182
           DD GA G    D  AF++AWK ACS    + +V P  + +LV PIE  GPC+S++TL+I+
Sbjct: 102 DDFGAKGNGADDTQAFVKAWKAACSSSGAMVLVVPQKN-YLVRPIEFSGPCKSQLTLQIY 161

Query: 183 GTINAPKDPNAWAGRNPRKWLYFRGVNHLTLEGGGEINGMGYEWWMTSCKINSTNPC-RH 242
           GTI A +D + +  ++   WL F  V +L + G G ING G  WW  SCKI    PC  +
Sbjct: 162 GTIEASEDRSIY--KDIDHWLIFDNVQNLLVVGPGTINGNGNIWWKNSCKIKPQPPCGTY 221

Query: 243 APTALTFHKCKNLKIHHLLVIDSQQMHVSFTSCLRVVASNLKVIAPGFSPNTDGIHISAS 302
           APTA+TF++C NL + +L + D+QQ+HV F +C+ V AS L V AP  SPNTDGIH++ +
Sbjct: 222 APTAVTFNRCNNLVVKNLNIQDAQQIHVIFQNCINVQASCLTVTAPEDSPNTDGIHVTNT 281

Query: 303 KGVTVKNSIISTGDDCISIVSNSSRVLIRDIVCGPGHGISIGSLGKYNTWGQVHNVRVDG 362
           + +T+ +S+I TGDDCISIVS S RV   DI CGPGHGISIGSLG+  +   V  V V+G
Sbjct: 282 QNITISSSVIGTGDDCISIVSGSQRVQATDITCGPGHGISIGSLGEDGSEDHVSGVFVNG 341

Query: 363 AVLSNTENGARIKTWQGGNGSATDITFKNMLMKNVSNPIIIDQYYCDSDT-PCANQTSAI 422
           A LS T NG RIKTW+GG+GSAT+I F+N+ M +V+NPIIIDQ YCD  T  C  Q SA+
Sbjct: 342 AKLSGTSNGLRIKTWKGGSGSATNIVFQNVQMNDVTNPIIIDQNYCDHKTKDCKQQKSAV 401

Query: 423 KVEDISFIHIKGTTATKEAIKFACSDKSPCEGLFLEDILLLSNDGE 466
           +V+++ + +I+GT+A+ +AI   CS   PC+G+ L+ + L +   E
Sbjct: 402 QVKNVLYQNIRGTSASGDAITLNCSQSVPCQGIVLQSVQLQNGRAE 444

[Close]

BLAST of Spg014356 vs. ExPASy TrEMBL
Match: A0A6J1JWW4 (probable polygalacturonase At1g80170 OS=Cucurbita maxima OX=3661 GN=LOC111488200 PE=3 SV=1)

HSP 1 Score: 788.5 bits (2035), Expect = 1.7e-224
Identity = 384/508 (75.59%), Postives = 408/508 (80.31%), Query Frame = 0


Query: 1   MKGQRLWPSPIPIFVFTLLSSLIFESSAEFDSLLQLPRSGSNTTRPRSKRVLYLGDFGAK 60
           MK  RLW SPIPIFVFT+L+ L FE SAEFDSLLQLP SG+N +R RSKRV YL DFGAK
Sbjct: 1   MKAHRLWRSPIPIFVFTVLARLSFECSAEFDSLLQLPLSGANRSRARSKRVFYLADFGAK 60

Query: 61  GDGISNDTQALLAAWKMACSFPTRTQIVFIASNTFLVHPIELHGPCSDYIVYFQAFYERR 120
           GDGISNDTQ L+                                                
Sbjct: 61  GDGISNDTQPLI------------------------------------------------ 120

Query: 121 LSDDDGAGFPFLDFPAFMEAWKMACSFPTRIRIVFPASDTFLVHPIELHGPCRSRVTLRI 180
                             EAWK+ACSFPTR RIVFPAS+TFLVHPIELHGPCRSRVTLRI
Sbjct: 121 ------------------EAWKIACSFPTRTRIVFPASNTFLVHPIELHGPCRSRVTLRI 180

Query: 181 HGTINAPKDPNAWAGRNPRKWLYFRGVNHLTLEGGGEINGMGYEWWMTSCKINSTNPCRH 240
           HGTINAPKDPN W  RNPRKWLYFR VNHLTL GGGEINGMGYEWWM SCKINSTNPCRH
Sbjct: 181 HGTINAPKDPNVWEHRNPRKWLYFRAVNHLTLNGGGEINGMGYEWWMRSCKINSTNPCRH 240

Query: 241 APTALTFHKCKNLKIHHLLVIDSQQMHVSFTSCLRVVASNLKVIAPGFSPNTDGIHISAS 300
           APTA+TFHKCKN+KIHHLLVIDSQQMHVSFT+CLRVVASNLKVIAP FSPNTDGIHISAS
Sbjct: 241 APTAITFHKCKNVKIHHLLVIDSQQMHVSFTNCLRVVASNLKVIAPAFSPNTDGIHISAS 300

Query: 301 KGVTVKNSIISTGDDCISIVSNSSRVLIRDIVCGPGHGISIGSLGKYNTWGQVHNVRVDG 360
           KGVTVKNS++STGDDCISIV+NSSR+LI+DI CGPGHGISIGSLGK NTW QVHN+RVDG
Sbjct: 301 KGVTVKNSVVSTGDDCISIVNNSSRILIKDIACGPGHGISIGSLGKQNTWAQVHNIRVDG 360

Query: 361 AVLSNTENGARIKTWQGGNGSATDITFKNMLMKNVSNPIIIDQYYCDSDTPCANQTSAIK 420
           AVLSNTENGARIKTWQGG+G ATDITFKNMLMKNVSNPIIIDQYYCDS+ PCANQTSA+K
Sbjct: 361 AVLSNTENGARIKTWQGGSGFATDITFKNMLMKNVSNPIIIDQYYCDSNHPCANQTSAVK 420

Query: 421 VEDISFIHIKGTTATKEAIKFACSDKSPCEGLFLEDILLLSNDGEMSTSFCWQAHGSNSG 480
           V DISFIHIKGTTAT EAIK ACSD  PC  L++ED+LLLSNDGEM TSFCWQAHGS+SG
Sbjct: 421 VRDISFIHIKGTTATNEAIKLACSDTLPCVDLYMEDVLLLSNDGEMLTSFCWQAHGSSSG 442

Query: 481 PVYPPPCFLGNENFIEQKVQPYPASHSF 509
           PVYPPPCFLGNE+FIEQKVQP PASHSF
Sbjct: 481 PVYPPPCFLGNESFIEQKVQPRPASHSF 442

[Close]

BLAST of Spg014356 vs. ExPASy TrEMBL
Match: A0A0A0KBV8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G433170 PE=3 SV=1)

HSP 1 Score: 783.1 bits (2021), Expect = 7.0e-223
Identity = 385/508 (75.79%), Postives = 410/508 (80.71%), Query Frame = 0


Query: 1   MKGQRLWPSPIPIFVFTLLSSLIFESSAEFDSLLQLPRSGSNTTRPRSKRVLYLGDFGAK 60
           MKGQRLWPS +PIF+FT+L+SL FE SA+FDSLLQLP S  N TRPRSK V YLGDFGAK
Sbjct: 1   MKGQRLWPSSLPIFLFTVLTSLTFECSADFDSLLQLPLSRLNITRPRSKGVFYLGDFGAK 60

Query: 61  GDGISNDTQALLAAWKMACSFPTRTQIVFIASNTFLVHPIELHGPCSDYIVYFQAFYERR 120
           GDGISNDTQAL                                                 
Sbjct: 61  GDGISNDTQAL------------------------------------------------- 120

Query: 121 LSDDDGAGFPFLDFPAFMEAWKMACSFPTRIRIVFPASDTFLVHPIELHGPCRSRVTLRI 180
                            MEAWKMACSFPTR+RIVFPAS+TFLVHP+ELHGPCRSRVTLRI
Sbjct: 121 -----------------MEAWKMACSFPTRVRIVFPASNTFLVHPLELHGPCRSRVTLRI 180

Query: 181 HGTINAPKDPNAWAGRNPRKWLYFRGVNHLTLEGGGEINGMGYEWWMTSCKINSTNPCRH 240
           HG INAPKDPNAWAG NPRKWLYFRGVNHLTLEGGGEINGMGYEWWM+SCKINSTNPCRH
Sbjct: 181 HGIINAPKDPNAWAGLNPRKWLYFRGVNHLTLEGGGEINGMGYEWWMSSCKINSTNPCRH 240

Query: 241 APTALTFHKCKNLKIHHLLVIDSQQMHVSFTSCLRVVASNLKVIAPGFSPNTDGIHISAS 300
           APTA+TFHKCKNLKIHHLLVIDSQQMHV+ TSCLRVVASNLKVIAP FSPNTDGIHISAS
Sbjct: 241 APTAITFHKCKNLKIHHLLVIDSQQMHVALTSCLRVVASNLKVIAPAFSPNTDGIHISAS 300

Query: 301 KGVTVKNSIISTGDDCISIVSNSSRVLIRDIVCGPGHGISIGSLGKYNTWGQVHNVRVDG 360
           KGV VKNSIISTGDDC+SIV NSSR+LI+DI CGPGHGISIGSLGK NT  QV NVRVDG
Sbjct: 301 KGVMVKNSIISTGDDCVSIVGNSSRILIKDITCGPGHGISIGSLGKRNTSAQVRNVRVDG 360

Query: 361 AVLSNTENGARIKTWQGGNGSATDITFKNMLMKNVSNPIIIDQYYCDSDTPCANQTSAIK 420
           AVLSNT+NGARIKTWQGG+GSATDI FKNMLMKNVSNPIIIDQYYCDS+ PCANQTSA+K
Sbjct: 361 AVLSNTKNGARIKTWQGGSGSATDIIFKNMLMKNVSNPIIIDQYYCDSNRPCANQTSAVK 420

Query: 421 VEDISFIHIKGTTATKEAIKFACSDKSPCEGLFLEDILLLSNDGEMSTSFCWQAHGSNSG 480
           VEDISFIHIKGTTAT +AIKFACSD SPC GL+LEDILLLS D ++STS CWQA+G +SG
Sbjct: 421 VEDISFIHIKGTTATNKAIKFACSDTSPCVGLYLEDILLLSEDAKISTSSCWQAYGLSSG 442

Query: 481 PVYPPPCFLGNENFIEQKVQPYPASHSF 509
           PVYPP CFLGNENFI+QKVQP P SHSF
Sbjct: 481 PVYPPSCFLGNENFIQQKVQPAPTSHSF 442

[Close]

BLAST of Spg014356 vs. ExPASy TrEMBL
Match: A0A6J1GP78 (probable polygalacturonase At1g80170 OS=Cucurbita moschata OX=3662 GN=LOC111455877 PE=3 SV=1)

HSP 1 Score: 780.8 bits (2015), Expect = 3.5e-222
Identity = 380/508 (74.80%), Postives = 406/508 (79.92%), Query Frame = 0


Query: 1   MKGQRLWPSPIPIFVFTLLSSLIFESSAEFDSLLQLPRSGSNTTRPRSKRVLYLGDFGAK 60
           M+  R W SPIPI VFT+L+ L FE SAEFDSLLQLP SG+N +R RSKRV YL DFGAK
Sbjct: 1   MEAHRFWRSPIPILVFTVLARLSFECSAEFDSLLQLPLSGANRSRSRSKRVFYLADFGAK 60

Query: 61  GDGISNDTQALLAAWKMACSFPTRTQIVFIASNTFLVHPIELHGPCSDYIVYFQAFYERR 120
           GDGISNDTQ L+                                                
Sbjct: 61  GDGISNDTQPLI------------------------------------------------ 120

Query: 121 LSDDDGAGFPFLDFPAFMEAWKMACSFPTRIRIVFPASDTFLVHPIELHGPCRSRVTLRI 180
                             EAWK ACSFPTR RIVFPAS+TFLVHPIELHGPCRSRVTLRI
Sbjct: 121 ------------------EAWKTACSFPTRTRIVFPASNTFLVHPIELHGPCRSRVTLRI 180

Query: 181 HGTINAPKDPNAWAGRNPRKWLYFRGVNHLTLEGGGEINGMGYEWWMTSCKINSTNPCRH 240
           HGTINAPKDPN W  RNPRKWLYFR VNHLTL GGGEINGMGY+WWM SCKINSTNPCRH
Sbjct: 181 HGTINAPKDPNVWERRNPRKWLYFRAVNHLTLNGGGEINGMGYKWWMRSCKINSTNPCRH 240

Query: 241 APTALTFHKCKNLKIHHLLVIDSQQMHVSFTSCLRVVASNLKVIAPGFSPNTDGIHISAS 300
           APTALTFHKCKNLKIHHLLVIDSQQMHVSFT+CLRVV SNLKVIAP FSPNTDGIHISAS
Sbjct: 241 APTALTFHKCKNLKIHHLLVIDSQQMHVSFTNCLRVVTSNLKVIAPAFSPNTDGIHISAS 300

Query: 301 KGVTVKNSIISTGDDCISIVSNSSRVLIRDIVCGPGHGISIGSLGKYNTWGQVHNVRVDG 360
           KGVTVKNS++STGDDCISIV+NSSR+LI+DI CGPGHGISIGSLGK NTW QVHN+RVDG
Sbjct: 301 KGVTVKNSVVSTGDDCISIVNNSSRILIKDIACGPGHGISIGSLGKQNTWAQVHNIRVDG 360

Query: 361 AVLSNTENGARIKTWQGGNGSATDITFKNMLMKNVSNPIIIDQYYCDSDTPCANQTSAIK 420
           AVLSNTENGARIKTWQGG+G ATDITFKNMLMKNVSNPIIIDQYYCDS+ PCANQTSA++
Sbjct: 361 AVLSNTENGARIKTWQGGSGFATDITFKNMLMKNVSNPIIIDQYYCDSNHPCANQTSAVE 420

Query: 421 VEDISFIHIKGTTATKEAIKFACSDKSPCEGLFLEDILLLSNDGEMSTSFCWQAHGSNSG 480
           V DISFIHIKGTTAT EAIKFACSD  PC  L++ED+LLLSNDGEM TSFCWQA+GS+SG
Sbjct: 421 VRDISFIHIKGTTATNEAIKFACSDTLPCVDLYMEDVLLLSNDGEMLTSFCWQANGSSSG 442

Query: 481 PVYPPPCFLGNENFIEQKVQPYPASHSF 509
           PVYPPPCFLGNE+FIEQKVQP+PASHSF
Sbjct: 481 PVYPPPCFLGNESFIEQKVQPHPASHSF 442

[Close]

BLAST of Spg014356 vs. ExPASy TrEMBL
Match: A0A1S3CF54 (probable polygalacturonase At1g80170 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499786 PE=3 SV=1)

HSP 1 Score: 778.1 bits (2008), Expect = 2.3e-221
Identity = 382/508 (75.20%), Postives = 409/508 (80.51%), Query Frame = 0


Query: 1   MKGQRLWPSPIPIFVFTLLSSLIFESSAEFDSLLQLPRSGSNTTRPRSKRVLYLGDFGAK 60
           MKGQRLWPS +PIF+FT+L+SL FE SAEFDSLLQLP S  NTTRPRSK + YLGDFGAK
Sbjct: 1   MKGQRLWPSSLPIFLFTVLTSLTFECSAEFDSLLQLPLSRLNTTRPRSKGIFYLGDFGAK 60

Query: 61  GDGISNDTQALLAAWKMACSFPTRTQIVFIASNTFLVHPIELHGPCSDYIVYFQAFYERR 120
           GDGISNDTQAL                                                 
Sbjct: 61  GDGISNDTQAL------------------------------------------------- 120

Query: 121 LSDDDGAGFPFLDFPAFMEAWKMACSFPTRIRIVFPASDTFLVHPIELHGPCRSRVTLRI 180
                            MEAWK+ACSFPTR RIVFPAS+TFLVHP+ELHGPCRSRVTLRI
Sbjct: 121 -----------------MEAWKVACSFPTRARIVFPASNTFLVHPLELHGPCRSRVTLRI 180

Query: 181 HGTINAPKDPNAWAGRNPRKWLYFRGVNHLTLEGGGEINGMGYEWWMTSCKINSTNPCRH 240
           HG INAPKDPNAWAG NPRKWLYF GVNHLTLEGGGEINGMGYEWWM+SCKINSTNPCRH
Sbjct: 181 HGIINAPKDPNAWAGLNPRKWLYFCGVNHLTLEGGGEINGMGYEWWMSSCKINSTNPCRH 240

Query: 241 APTALTFHKCKNLKIHHLLVIDSQQMHVSFTSCLRVVASNLKVIAPGFSPNTDGIHISAS 300
           APTA+TFHKCKN+KIHHLLVIDSQQMHV+FT+CLRVVASNLKVIAP FSPNTDGIHISAS
Sbjct: 241 APTAITFHKCKNVKIHHLLVIDSQQMHVAFTNCLRVVASNLKVIAPAFSPNTDGIHISAS 300

Query: 301 KGVTVKNSIISTGDDCISIVSNSSRVLIRDIVCGPGHGISIGSLGKYNTWGQVHNVRVDG 360
           +GV VKNSIISTGDDC+SIV NSSR+LI+DI CGPGHGISIGSLGK NT  QV NVRVDG
Sbjct: 301 RGVMVKNSIISTGDDCVSIVGNSSRILIKDIACGPGHGISIGSLGKQNTSAQVRNVRVDG 360

Query: 361 AVLSNTENGARIKTWQGGNGSATDITFKNMLMKNVSNPIIIDQYYCDSDTPCANQTSAIK 420
           AVLSNT+NGARIKTWQGG+GSATDITFKNMLMKNVSNPIIIDQYYCDS+ PCANQTSA+K
Sbjct: 361 AVLSNTKNGARIKTWQGGSGSATDITFKNMLMKNVSNPIIIDQYYCDSNLPCANQTSAVK 420

Query: 421 VEDISFIHIKGTTATKEAIKFACSDKSPCEGLFLEDILLLSNDGEMSTSFCWQAHGSNSG 480
           VE+ SFIHIKGTTAT EAIKFACSD  PC GL+LEDILLLS D E+STS CWQA+G +SG
Sbjct: 421 VENTSFIHIKGTTATNEAIKFACSDTLPCVGLYLEDILLLSKDAEISTSSCWQAYGLSSG 442

Query: 481 PVYPPPCFLGNENFIEQKVQPYPASHSF 509
           PVYPP CFLGNENFI+QKVQP P SHSF
Sbjct: 481 PVYPPSCFLGNENFIQQKVQPDPTSHSF 442

[Close]

BLAST of Spg014356 vs. ExPASy TrEMBL
Match: A0A6J1DK85 (probable polygalacturonase At1g80170 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111021679 PE=3 SV=1)

HSP 1 Score: 771.5 bits (1991), Expect = 2.1e-219
Identity = 383/508 (75.39%), Postives = 404/508 (79.53%), Query Frame = 0


Query: 1   MKGQRLWPSPIPIFVFTLLSSLIFESSAEFDSLLQLPRSGSNTTRPRSKRVLYLGDFGAK 60
           MKGQ   P P+PIFVF + +S  FES+AEFDSLLQLP SGSN TRP+SKRV  L DFGAK
Sbjct: 15  MKGQGSQPFPLPIFVFIVFTSFSFESTAEFDSLLQLPLSGSNATRPKSKRVFDLRDFGAK 74

Query: 61  GDGISNDTQALLAAWKMACSFPTRTQIVFIASNTFLVHPIELHGPCSDYIVYFQAFYERR 120
           GDG SNDTQALLAAWKMACSFPTRT                                   
Sbjct: 75  GDGFSNDTQALLAAWKMACSFPTRT----------------------------------- 134

Query: 121 LSDDDGAGFPFLDFPAFMEAWKMACSFPTRIRIVFPASDTFLVHPIELHGPCRSRVTLRI 180
                                          R+VFPAS+TFLVHPIELHGPCRSR+TLRI
Sbjct: 135 -------------------------------RVVFPASNTFLVHPIELHGPCRSRLTLRI 194

Query: 181 HGTINAPKDPNAWAGRNPRKWLYFRGVNHLTLEGGGEINGMGYEWWMTSCKINSTNPCRH 240
           +GTINAPKDP+AWAGRNPRKWLYFRGVNHLTLEGGGEINGMGYEWWM SCKINSTNPCRH
Sbjct: 195 YGTINAPKDPDAWAGRNPRKWLYFRGVNHLTLEGGGEINGMGYEWWMRSCKINSTNPCRH 254

Query: 241 APTALTFHKCKNLKIHHLLVIDSQQMHVSFTSCLRVVASNLKVIAPGFSPNTDGIHISAS 300
           APTA+TFHKCKNLKI HLLVIDSQQMHVSFT+CLRVVAS+LKVIAP FSPNTDGIHISAS
Sbjct: 255 APTAMTFHKCKNLKIRHLLVIDSQQMHVSFTNCLRVVASDLKVIAPAFSPNTDGIHISAS 314

Query: 301 KGVTVKNSIISTGDDCISIVSNSSRVLIRDIVCGPGHGISIGSLGKYNTWGQVHNVRVDG 360
           +GVTV NSIISTGDDCISIVSNSSRVLIR+I CGPGHGISIGSLGK NTW QV NV VDG
Sbjct: 315 RGVTVTNSIISTGDDCISIVSNSSRVLIRNIACGPGHGISIGSLGKQNTWAQVRNVMVDG 374

Query: 361 AVLSNTENGARIKTWQGGNGSATDITFKNMLMKNVSNPIIIDQYYCDSDTPCANQTSAIK 420
           AVLSNT+NG RIKTWQGG+G ATDITFKN+LMKNVSNPIIIDQYYCDSD PCANQTSA+K
Sbjct: 375 AVLSNTKNGVRIKTWQGGSGFATDITFKNILMKNVSNPIIIDQYYCDSDLPCANQTSAVK 434

Query: 421 VEDISFIHIKGTTATKEAIKFACSDKSPCEGLFLEDILLLSNDGEMSTSFCWQAHGSNSG 480
           V DISFIHIKGT ATKEAIKFACSD SPCE LFLEDILLLSNDG++ TS CWQA GSNSG
Sbjct: 435 VGDISFIHIKGTAATKEAIKFACSDTSPCEDLFLEDILLLSNDGDILTSLCWQAQGSNSG 456

Query: 481 PVYPPPCFLGNENFIEQKVQPYPASHSF 509
           PVYPP CFL NENFIEQ VQP P SHSF
Sbjct: 495 PVYPPSCFLSNENFIEQVVQPDPVSHSF 456

[Close]

BLAST of Spg014356 vs. TAIR 10
Match: AT1G80170.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 527.7 bits (1358), Expect = 1.0e-149
Identity = 254/490 (51.84%), Postives = 334/490 (68.16%), Query Frame = 0


Query: 13  IFVFTLLSSLIFESSA-EFDSLLQLPRSGSNTTRPRSKRVLYLGDFGAKGDGISNDTQAL 72
           + +  + SSL   ++A  F+SLLQLPR  S +TRPRS+R+L++G+FGAKG+G+++DT+  
Sbjct: 13  LLLLVVASSLALTANANSFESLLQLPRRQSRSTRPRSERLLHVGNFGAKGNGVTDDTK-- 72

Query: 73  LAAWKMACSFPTRTQIVFIASNTFLVHPIELHGPCSDYIVYFQAFYERRLSDDDGAGFPF 132
                                                                       
Sbjct: 73  ------------------------------------------------------------ 132

Query: 133 LDFPAFMEAWKMACSFPTRIRIVFPASDTFLVHPIELHGPCRSRVTLRIHGTINAPKDPN 192
               AF +AWK ACS   + RI+ P + T L+ PI+L GPC++R+TL+I GTI AP DP+
Sbjct: 133 ----AFADAWKTACSSKVKTRILVPENYTCLLRPIDLSGPCKARLTLQISGTIIAPNDPD 192

Query: 193 AWAGRNPRKWLYFRGVNHLTLEGGGEINGMGYEWWMTSCKINSTNPCRHAPTALTFHKCK 252
            W G N RKWLYF G++ LT+EGGG +NGMG EWW  SCK N +NPCR APTALTFHKCK
Sbjct: 193 VWEGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCKHNHSNPCRGAPTALTFHKCK 252

Query: 253 NLKIHHLLVIDSQQMHVSFTSCLRVVASNLKVIAPGFSPNTDGIHISASKGVTVKNSIIS 312
           N+++ +L VIDSQQMH++ TSC RV  S LKVIAP  SPNTDGIHIS S+G+ + N+ +S
Sbjct: 253 NMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPATSPNTDGIHISVSRGIVIDNTTVS 312

Query: 313 TGDDCISIVSNSSRVLIRDIVCGPGHGISIGSLGKYNTWGQVHNVRVDGAVLSNTENGAR 372
           TGDDCISIV NS+++ I +I+CGPGHGISIGSLGK  +W +V ++ VD A++S+T NG R
Sbjct: 313 TGDDCISIVKNSTQISISNIICGPGHGISIGSLGKSKSWEEVRDITVDTAIISDTANGVR 372

Query: 373 IKTWQGGNGSATDITFKNMLMKNVSNPIIIDQYYCDSDTPCANQTSAIKVEDISFIHIKG 432
           IKTWQGG+G  + I F+N+ M NVSNPIIIDQYYCDS  PCANQTSAI +E+ISF+H++G
Sbjct: 373 IKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDSRKPCANQTSAISIENISFVHVRG 432

Query: 433 TTATKEAIKFACSDKSPCEGLFLEDILLLSNDGE-MSTSFCWQAHGSNSGPVYPPPCFLG 492
           T+A+KEAIK +CSD SPC  + L+DI L  ++G+  + SFCW+A+GS+SG VYPPPC   
Sbjct: 433 TSASKEAIKISCSDSSPCRNILLQDIDLEPSNGDGFTESFCWEAYGSSSGQVYPPPCLSD 436

Query: 493 NENFIEQKVQ 501
           + +F+EQ VQ
Sbjct: 493 DTSFLEQSVQ 436

[Close]

BLAST of Spg014356 vs. TAIR 10
Match: AT1G70500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 343.6 bits (880), Expect = 2.7e-94
Identity = 166/352 (47.16%), Postives = 233/352 (66.19%), Query Frame = 0


Query: 133 DFPAFMEAWKMACSFPTRIRIVFPASDTFLVHPIELHGPCRSRVTLRIHGTINAPKDPNA 192
           D  AF+ AW MACS P  + +V P   ++LV+  +  GPC+ ++ ++I GTI AP +P+ 
Sbjct: 82  DTQAFIRAWTMACSAPNSVLLV-PQGRSYLVNATKFDGPCQEKLIIQIDGTIIAPDEPSQ 141

Query: 193 WAGRNPRKWLYFRGVNHLTLEGGGEINGMGYEWWMTSCKINSTNPCRHAPTALTFHKCKN 252
           W  + PR WL F  +  +  +G G I+G G +WW  SCK N +NPC  APTALT +   N
Sbjct: 142 WDPKFPRNWLQFSKLQGVVFQGNGVIDGSGTKWWAASCKKNKSNPCVGAPTALTIYSSSN 201

Query: 253 LKIHHLLVIDSQQMHVSFTSCLRVVASNLKVIAPGFSPNTDGIHISASKGVTVKNSIIST 312
           + +  L + +SQQMH+       V  S + V +PG SPNTDGIHI+AS  V V++S IST
Sbjct: 202 VYVRGLTIRNSQQMHLIIQRSTTVRISRVMVTSPGDSPNTDGIHITASTDVVVQDSKIST 261

Query: 313 GDDCISIVSNSSRVLIRDIVCGPGHGISIGSLGKYNTWGQVHNVRVDGAVLSNTENGARI 372
           GDDC+SIV+ S+++ ++ I CGPGHGISIGSLG+ ++ G V  V ++ A L NT NG RI
Sbjct: 262 GDDCVSIVNGSAKIKMKRIYCGPGHGISIGSLGQGHSKGTVTAVVLETAFLKNTTNGLRI 321

Query: 373 KTWQGGNGSATDITFKNMLMKNVSNPIIIDQYYCDSDTPCANQTSAIKVEDISFIHIKGT 432
           KTWQGGNG    + F+N++M++V+NPIIIDQ+YCDS + C NQTSA+ + +I + +I GT
Sbjct: 322 KTWQGGNGYVKGVRFENVVMQDVANPIIIDQFYCDSPSTCQNQTSAVHISEIMYRNITGT 381

Query: 433 TATKEAIKFACSDKSPCEGLFLEDILLLSNDGEMSTSFCWQAHGSNSGPVYP 485
           T + +AI F CSD  PC  + L +I L  NDG++  ++C  A G   G V+P
Sbjct: 382 TKSSKAINFKCSDAVPCSHIVLNNINLEGNDGKVE-AYCNSAEGFGYGVVHP 431

[Close]

BLAST of Spg014356 vs. TAIR 10
Match: AT1G23460.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 327.4 bits (838), Expect = 2.0e-89
Identity = 162/352 (46.02%), Postives = 225/352 (63.92%), Query Frame = 0


Query: 133 DFPAFMEAWKMACSFPTRIRIVFPASDTFLVHPIELHGPCRSRVTLRIHGTINAPKDPNA 192
           D  AF+ AW  ACS    + +V P    +LV+  + +GPC  ++ ++I GTI AP +P+ 
Sbjct: 82  DTQAFVSAWSKACSTSKSVFLV-PEGRRYLVNATKFNGPCEQKLIIQIDGTIVAPDEPSN 141

Query: 193 WAGRNPRKWLYFRGVNHLTLEGGGEINGMGYEWWMTSCKINSTNPCRHAPTALTFHKCKN 252
           W  +  R WL F  +  +  +G G I+G G +WW  SCK N +NPC+ APTALT      
Sbjct: 142 WDSKFQRIWLEFSKLKGVVFQGKGVIDGSGSKWWAASCKKNKSNPCKSAPTALTIESSSG 201

Query: 253 LKIHHLLVIDSQQMHVSFTSCLRVVASNLKVIAPGFSPNTDGIHISASKGVTVKNSIIST 312
           +K+  L + +SQQM+        V  S + V +PG SPNTDGIHI+ S  V +++  I T
Sbjct: 202 VKVSGLTIQNSQQMNFIIARSDSVRVSKVMVSSPGDSPNTDGIHITGSTNVILQDCKIGT 261

Query: 313 GDDCISIVSNSSRVLIRDIVCGPGHGISIGSLGKYNTWGQVHNVRVDGAVLSNTENGARI 372
           GDDC+SIV+ SS + +++I CGPGHGISIGSLGK NT G V  V +D A+L  T NG RI
Sbjct: 262 GDDCVSIVNASSNIKMKNIYCGPGHGISIGSLGKDNTTGIVTQVVLDTALLRETTNGLRI 321

Query: 373 KTWQGGNGSATDITFKNMLMKNVSNPIIIDQYYCDSDTPCANQTSAIKVEDISFIHIKGT 432
           KT+QGG+G    I F N+ M++V+NPI+IDQ+YCDS T C NQTSA+K+  I + +I GT
Sbjct: 322 KTYQGGSGYVQGIRFTNVEMQDVANPILIDQFYCDSPTTCQNQTSAVKISQIMYRNITGT 381

Query: 433 TATKEAIKFACSDKSPCEGLFLEDILLLSNDGEMSTSFCWQAHGSNSGPVYP 485
           T + +AIKFACSD  PC  + L ++ L  NDG++  ++C  A G   G ++P
Sbjct: 382 TKSAKAIKFACSDTVPCSHIVLNNVNLEGNDGQVE-AYCNSAEGFGYGVIHP 431

[Close]

BLAST of Spg014356 vs. TAIR 10
Match: AT3G07970.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 323.2 bits (827), Expect = 3.8e-88
Identity = 159/354 (44.92%), Postives = 230/354 (64.97%), Query Frame = 0


Query: 133 DFPAFMEAWKMACSFPTRIRIVFPASDTFLVHPIELHGPCRSR-VTLRIHGTINAPKDPN 192
           D  AFM+AW+ ACS    + IV P +  +++  +   GPC+S  +  +I+G I A ++P+
Sbjct: 85  DSKAFMKAWEAACSSTGIVYIVAPKNRDYMLKAVTFSGPCKSSLIIFKIYGRIEAWENPS 144

Query: 193 AWAGRNPRKWLYFRGVNHLTLEGGGEINGMGYEWWMTSCKINSTNPCRHAPTALTFHKCK 252
            +  R  R W+ F  VN+L +EGGG I+G G+ WW  SCKIN   PC  APTA+TF +C 
Sbjct: 145 DYKER--RHWIVFENVNNLRVEGGGRIDGNGHIWWPKSCKINPQLPCLGAPTAVTFVECN 204

Query: 253 NLKIHHLLVIDSQQMHVSFTSCLRVVASNLKVIAPGFSPNTDGIHISASKGVTVKNSIIS 312
           NL++ ++ + ++QQMH++F  C  V A NL V +P  SPNTDGIH+S ++ + +++SI+ 
Sbjct: 205 NLRVSNIRLENAQQMHLTFQDCKNVKALNLMVTSPADSPNTDGIHVSGTQNILIQDSIVR 264

Query: 313 TGDDCISIVSNSSRVLIRDIVCGPGHGISIGSLGKYNTWGQVHNVRVDGAVLSNTENGAR 372
           TGDDCISIVS S  V    I CGPGHGISIGSLG+ N+   V NV V+ A L  T NG R
Sbjct: 265 TGDDCISIVSGSENVRATGITCGPGHGISIGSLGEDNSEAYVSNVVVNKATLIGTTNGVR 324

Query: 373 IKTWQGGNGSATDITFKNMLMKNVSNPIIIDQYYCDSDTPCANQTSAIKVEDISFIHIKG 432
           IKTWQGG+G A +I F++++MKNV+NPIII+Q YCD    C  Q SA++V ++ + +I+G
Sbjct: 325 IKTWQGGHGMAKNIIFQDIIMKNVTNPIIINQDYCDRVEACPEQKSAVQVSNVLYKNIQG 384

Query: 433 TTATKEAIKFACSDKSPCEGLFLEDILLL-SNDGEMSTSFCWQAHGSNSGPVYP 485
           T++   A+KF CS   PC G+ ++++ L+     ++S + C        G V P
Sbjct: 385 TSSRPIAVKFVCSKNIPCRGISMQNVKLVDQTQQDVSKASCSNVKLDTRGNVSP 436

[Close]

BLAST of Spg014356 vs. TAIR 10
Match: AT2G41850.1 (polygalacturonase abscission zone A. thaliana )

HSP 1 Score: 320.5 bits (820), Expect = 2.5e-87
Identity = 165/354 (46.61%), Postives = 231/354 (65.25%), Query Frame = 0


Query: 133 DFPAFMEAWKMACSFPTRIRIVFPASDTFLVHPIELHGPCRSRVTLRIHGTINAPKDPNA 192
           D  AF+ AWK ACS    + ++ P  +T+L+  I+L GPC S +T++I GT++A +  + 
Sbjct: 83  DTQAFVNAWKKACSSNGAVNLLVPKGNTYLLKSIQLTGPCNSILTVQIFGTLSASQKRSD 142

Query: 193 WAGRNPRKWLYFRGVNHLTLEGG--GEINGMGYEWWMTSCKINSTNPCRHAPTALTFHKC 252
           +  ++  KW+ F GVN+L+++GG  G ++G G  WW  SCK N   PC  APTALTF+  
Sbjct: 143 Y--KDISKWIMFDGVNNLSVDGGDTGVVDGNGETWWQNSCKRNKAKPCTKAPTALTFYNS 202

Query: 253 KNLKIHHLLVIDSQQMHVSFTSCLRVVASNLKVIAPGFSPNTDGIHISASKGVTVKNSII 312
           K+L + +L V ++QQ+ +S   C  V  SN+ V AP  SPNTDGIHI+ ++ + V  SII
Sbjct: 203 KSLIVKNLKVRNAQQIQISIEKCSNVQVSNVVVTAPADSPNTDGIHITNTQNIRVSESII 262

Query: 313 STGDDCISIVSNSSRVLIRDIVCGPGHGISIGSLGKYNTWGQVHNVRVDGAVLSNTENGA 372
            TGDDCISI S S  V I DI CGPGHGISIGSLG  N+   V  V VDGA LS T+NG 
Sbjct: 263 GTGDDCISIESGSQNVQINDITCGPGHGISIGSLGDDNSKAFVSGVTVDGAKLSGTDNGV 322

Query: 373 RIKTWQGGNGSATDITFKNMLMKNVSNPIIIDQYYCDSDTPCANQTSAIKVEDISFIHIK 432
           RIKT+QGG+G+A++I F+N+ M NV NPIIIDQ YCD  + C  + SA++V+++ +  I 
Sbjct: 323 RIKTYQGGSGTASNIIFQNIQMDNVKNPIIIDQDYCDK-SKCTTEKSAVQVKNVVYRDIS 382

Query: 433 GTTATKEAIKFACSDKSPCEGLFLEDILLLSNDGEMSTSFCWQAHGSNSGPVYP 485
           GT+A++ AI F CS   PC+G+ L+ + +    G  +T  C  A+  + G V P
Sbjct: 383 GTSASENAITFNCSKNYPCQGIVLDRVNI---KGGKAT--CTNANVVDKGAVLP 428

The following BLAST results are available for this feature:

BLAST of Spg014356 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023547734.1	5.7e-227	76.57	probable polygalacturonase At1g80170 [Cucurbita pepo subsp. pepo]	[more]
XP_038897077.1	7.4e-227	77.76	probable polygalacturonase At1g80170 [Benincasa hispida]	[more]
XP_022991643.1	3.5e-224	75.59	probable polygalacturonase At1g80170 [Cucurbita maxima]	[more]
KAG7014128.1	3.8e-223	75.20	putative polygalacturonase [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6575587.1	8.5e-223	75.20	putative polygalacturonase, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Spg014356 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94AJ5	1.4e-148	51.84	Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017...	[more]
Q02096	3.8e-101	51.43	Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1	[more]
P35336	7.5e-97	49.31	Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1	[more]
P05117	7.0e-95	49.00	Polygalacturonase-2 OS=Solanum lycopersicum OX=4081 GN=PG2 PE=1 SV=1	[more]
P48978	2.6e-89	49.42	Polygalacturonase OS=Malus domestica OX=3750 PE=2 SV=1	[more]

BLAST of Spg014356 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JWW4	1.7e-224	75.59	probable polygalacturonase At1g80170 OS=Cucurbita maxima OX=3661 GN=LOC111488200...	[more]
A0A0A0KBV8	7.0e-223	75.79	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G433170 PE=3 SV=1	[more]
A0A6J1GP78	3.5e-222	74.80	probable polygalacturonase At1g80170 OS=Cucurbita moschata OX=3662 GN=LOC1114558...	[more]
A0A1S3CF54	2.3e-221	75.20	probable polygalacturonase At1g80170 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A6J1DK85	2.1e-219	75.39	probable polygalacturonase At1g80170 isoform X2 OS=Momordica charantia OX=3673 G...	[more]

BLAST of Spg014356 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G80170.1	1.0e-149	51.84	Pectin lyase-like superfamily protein	[more]
AT1G70500.1	2.7e-94	47.16	Pectin lyase-like superfamily protein	[more]
AT1G23460.1	2.0e-89	46.02	Pectin lyase-like superfamily protein	[more]
AT3G07970.1	3.8e-88	44.92	Pectin lyase-like superfamily protein	[more]
AT2G41850.1	2.5e-87	46.61	polygalacturonase abscission zone A. thaliana	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 382..403 e-value: 5300.0 score: 2.0 coord: 273..299 e-value: 2300.0 score: 4.6 coord: 323..343 e-value: 1500.0 score: 6.1 coord: 300..321 e-value: 47.0 score: 12.9 coord: 353..374 e-value: 2100.0 score: 5.0
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 145..468 e-value: 1.3E-95 score: 320.2
IPR000743	Glycoside hydrolase, family 28	PROSITE	PS00502	POLYGALACTURONASE	coord: 330..343
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 130..487 e-value: 3.7E-108 score: 364.1 coord: 39..129 e-value: 2.8E-10 score: 41.7
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 16..110 coord: 133..495
None	No IPR available	PANTHER	PTHR31375:SF108	GLYCOSIDE HYDROLASE, FAMILY 28	coord: 16..110 coord: 133..495
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 133..489
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 51..115

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg014356.1	Spg014356.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity