Spg014302 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg014302
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionisoflavone reductase-like protein
Locationscaffold3: 34298632 .. 34299780 (+)
RNA-Seq ExpressionSpg014302
SyntenySpg014302
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CAGCCATCGCCAAAATTTGGTTTGCTTCTTCGCCTAAAATTTGGATGAAAAGAAATGGGAGGCGAAAGCAAAAGCAAGATTCTGTTCATAGGAAGCAGTGGTTACATCGGTAAGTTTGTGGTGGAGGCGAGCGCCAAGGCTGGCCATCCCACCTATGTTCTTCTCAGAGATTCCATTCTCTCTGATCCTTCCAAATCTCACATCATCAACACTTTCAAGAGTTTAAGAGTCATTTTTATATCTGTAAGTGCAATTTCAAACCTCTGATTATATTGTTGAGAAATTAAAATTTGTGGAATTTGATCTTCTTTACTTGTAATTTGTAGGGCGATCTCTACGATCATGAGAGTCTGGTTAAGGCCATAAAACAAGTGGATGTGGTGATATCGACGGTCAGTCACAGTCAGTTAGCCAAGCAAGATAGGATCGTCTCGGCCATGAAAGAAGCAGGCAATGTTAAGAGATTTTTCCCTTCTGAGTATGGGAACGATGTGGATCGTGTGCATGGCGTTGAGCCAGCAAAATCAGCCTTCGCTGCAAAGGCAAAAATTCGTCGAGCTGTGGAGGTTGCAAAAATTCCCTATACCTTTGTATCCTCCAACTTTTGTTCTGGTTGGTTCCTTCCCTCTCTTGCTCAGCCTCAGCCTCAAGCCTCCACTCCACCTAAGGATAAGGTTGTTATCTTAGGGGATGGAAATTCCAAGGGGGTAAGAATTAAACTCCTTTGCCCAATAAAGTGTAGAACAATAGATAAAACGATCCTGATATCATATTAGATAACACGAGGTTCCATCTCAAAATCAATTGACAATTAGTAGAGTAACTAATGTATCTTATATATTGTGAGTTCTCCACTTATCTTTTCAATGTGAGATCCTCAACAAGCACAACTTATTGGTTTCATTTTCTTTTTAATTTTCTTTTTATTTTTCATTATCAGTAATATTCAAGGAGGAGCATGATATTGGAACCTATATTATTAGAGCTGTGGATGATCCAAGAACTTTGAACAAAATCCTCTACGTTAGACCTTCCCAAAACACTTACTCTTTCATTGATCTTGTGTCTCTTTGGGAGAAAAAATGTGGAAAAATCCTCGAGAAGACCTATGTTACCGAGGAACAATTGTTGAAGAACATCCAAGGTTAG

mRNA sequence

ATGGGAGGCGAAAGCAAAAGCAAGATTCTGTTCATAGGAAGCAGTGGTTACATCGGTAAGTTTGTGGTGGAGGCGAGCGCCAAGGCTGGCCATCCCACCTATGTTCTTCTCAGAGATTCCATTCTCTCTGATCCTTCCAAATCTCACATCATCAACACTTTCAAGAGTTTAAGAGTCATTTTTATATCTGGCGATCTCTACGATCATGAGAGTCTGGTTAAGGCCATAAAACAAGTGGATGTGGTGATATCGACGGTCAGTCACAGTCAGTTAGCCAAGCAAGATAGGATCGTCTCGGCCATGAAAGAAGCAGGCAATGTTAAGAGATTTTTCCCTTCTGAGTATGGGAACGATGTGGATCGTGTGCATGGCGTTGAGCCAGCAAAATCAGCCTTCGCTGCAAAGGCAAAAATTCGTCGAGCTGTGGAGGTTGCAAAAATTCCCTATACCTTTGTATCCTCCAACTTTTGTTCTGGTTGGTTCCTTCCCTCTCTTGCTCAGCCTCAGCCTCAAGCCTCCACTCCACCTAAGGATAAGGTTGTTATCTTAGGGGATGGAAATTCCAAGGGGGAGGAGCATGATATTGGAACCTATATTATTAGAGCTGTGGATGATCCAAGAACTTTGAACAAAATCCTCTACGTTAGACCTTCCCAAAACACTTACTCTTTCATTGATCTTGTGTCTCTTTGGGAGAAAAAATGTGGAAAAATCCTCGAGAAGACCTATGTTACCGAGGAACAATTGTTGAAGAACATCCAAGGTTAG

Coding sequence (CDS)

ATGGGAGGCGAAAGCAAAAGCAAGATTCTGTTCATAGGAAGCAGTGGTTACATCGGTAAGTTTGTGGTGGAGGCGAGCGCCAAGGCTGGCCATCCCACCTATGTTCTTCTCAGAGATTCCATTCTCTCTGATCCTTCCAAATCTCACATCATCAACACTTTCAAGAGTTTAAGAGTCATTTTTATATCTGGCGATCTCTACGATCATGAGAGTCTGGTTAAGGCCATAAAACAAGTGGATGTGGTGATATCGACGGTCAGTCACAGTCAGTTAGCCAAGCAAGATAGGATCGTCTCGGCCATGAAAGAAGCAGGCAATGTTAAGAGATTTTTCCCTTCTGAGTATGGGAACGATGTGGATCGTGTGCATGGCGTTGAGCCAGCAAAATCAGCCTTCGCTGCAAAGGCAAAAATTCGTCGAGCTGTGGAGGTTGCAAAAATTCCCTATACCTTTGTATCCTCCAACTTTTGTTCTGGTTGGTTCCTTCCCTCTCTTGCTCAGCCTCAGCCTCAAGCCTCCACTCCACCTAAGGATAAGGTTGTTATCTTAGGGGATGGAAATTCCAAGGGGGAGGAGCATGATATTGGAACCTATATTATTAGAGCTGTGGATGATCCAAGAACTTTGAACAAAATCCTCTACGTTAGACCTTCCCAAAACACTTACTCTTTCATTGATCTTGTGTCTCTTTGGGAGAAAAAATGTGGAAAAATCCTCGAGAAGACCTATGTTACCGAGGAACAATTGTTGAAGAACATCCAAGGTTAG

Protein sequence

MGGESKSKILFIGSSGYIGKFVVEASAKAGHPTYVLLRDSILSDPSKSHIINTFKSLRVIFISGDLYDHESLVKAIKQVDVVISTVSHSQLAKQDRIVSAMKEAGNVKRFFPSEYGNDVDRVHGVEPAKSAFAAKAKIRRAVEVAKIPYTFVSSNFCSGWFLPSLAQPQPQASTPPKDKVVILGDGNSKGEEHDIGTYIIRAVDDPRTLNKILYVRPSQNTYSFIDLVSLWEKKCGKILEKTYVTEEQLLKNIQG
Homology
BLAST of Spg014302 vs. NCBI nr
Match: XP_038888446.1 (phenylcoumaran benzylic ether reductase POP1-like [Benincasa hispida])

HSP 1 Score: 416.0 bits (1068), Expect = 2.3e-112
Identity = 209/257 (81.32%), Postives = 228/257 (88.72%), Query Frame = 0

Query: 1   MGGESKSKILFIGSSGYIGKFVVEASAKAGHPTYVLLRDSILSDPSKSHIINTFKSLRVI 60
           M  +SKSKILFIG SGYIGKF+VEAS KAGHPTY LLRDS LS+P+KSHIIN FKSL VI
Sbjct: 1   MATQSKSKILFIGGSGYIGKFMVEASTKAGHPTYALLRDSTLSNPTKSHIINHFKSLNVI 60

Query: 61  FISGDLYDHESLVKAIKQVDVVISTVSHSQLAKQDRIVSAMKEAGNVKRFFPSEYGNDVD 120
           F+SGDL DHESLVKAIKQVDVVIST+ H  LA QDRIVSA+KEAGNVKRFFPSEYG DVD
Sbjct: 61  FVSGDLCDHESLVKAIKQVDVVISTIGHDLLADQDRIVSAIKEAGNVKRFFPSEYGTDVD 120

Query: 121 RVHGVEPAKSAFAAKAKIRRAVEVAKIPYTFVSSNFCSGWFLPSLAQPQPQASTPPKDKV 180
           RVHGVEPAKS F AKA+IRRAVE A IPYT+VSSNFCSGWFLPSLAQP  +ASTPPKDKV
Sbjct: 121 RVHGVEPAKSLFNAKAQIRRAVEAANIPYTYVSSNFCSGWFLPSLAQPYLEASTPPKDKV 180

Query: 181 VILGDGNSK---GEEHDIGTYIIRAVDDPRTLNKILYVRPSQNTYSFIDLVSLWEKKCGK 240
            ILGDGNSK    +EHDI TYII+AVDD RTLNKILYV+P +N YS++DLVSLWEKKCGK
Sbjct: 181 FILGDGNSKVIFNDEHDIATYIIKAVDDSRTLNKILYVKPLRNIYSYVDLVSLWEKKCGK 240

Query: 241 ILEKTYVTEEQLLKNIQ 255
           ILEKTYV++EQLLKNI+
Sbjct: 241 ILEKTYVSDEQLLKNIR 257

BLAST of Spg014302 vs. NCBI nr
Match: XP_016900450.1 (PREDICTED: isoflavone reductase homolog isoform X2 [Cucumis melo])

HSP 1 Score: 396.0 bits (1016), Expect = 2.5e-106
Identity = 203/259 (78.38%), Postives = 223/259 (86.10%), Query Frame = 0

Query: 1   MGGESKSKILFIGSSGYIGKFVVEASAKAGHPTYVLLRDSILSDPSKSHIINTFKSLRVI 60
           M  +SK KILFIGS+GY+GKFVVEASAKAGHPTY LL DS LSDP+KSHIIN FKSL+VI
Sbjct: 4   MATQSKCKILFIGSTGYVGKFVVEASAKAGHPTYALLSDSTLSDPTKSHIINHFKSLKVI 63

Query: 61  FISGDLYDHESLVKAIKQVDVVISTVSHSQLAKQDRIVSAMKEAGNVKRFFPSEYGNDVD 120
           F+SG+LYDHESLVKAIKQVDVVISTV +   A Q RI+SA+KEAGNV+RF PSEYGNDVD
Sbjct: 64  FVSGNLYDHESLVKAIKQVDVVISTVGNDLFADQVRIISAIKEAGNVRRFLPSEYGNDVD 123

Query: 121 RVHGVEPAKSAFAAKAKIRRAVEVAKIPYTFVSSNFCSGWFLPSLAQPQP-QASTPPKDK 180
           RVHGVEPAKS F AKA+IR+AV+ A IP+TF+SSNFCS WFLPSLAQP    AS PPKDK
Sbjct: 124 RVHGVEPAKSLFNAKAQIRQAVKDANIPFTFISSNFCSAWFLPSLAQPYDLNASAPPKDK 183

Query: 181 VVILGDGNSK---GEEHDIGTYIIRAVDDPRTLNKILYVRPSQNTYSFIDLVSLWEKKCG 240
           V ILGDG SK    EEHDI TYII+AVDD RTLNKILYVRP QN YS++DLVSLWEKKCG
Sbjct: 184 VFILGDGISKVIFNEEHDIATYIIKAVDDSRTLNKILYVRPLQNIYSYVDLVSLWEKKCG 243

Query: 241 KILEKTYVTEEQLLKNIQG 256
            ILEK YV+EEQLLKNIQG
Sbjct: 244 IILEKIYVSEEQLLKNIQG 262

BLAST of Spg014302 vs. NCBI nr
Match: XP_016900451.1 (PREDICTED: isoflavone reductase-like protein isoform X3 [Cucumis melo])

HSP 1 Score: 396.0 bits (1016), Expect = 2.5e-106
Identity = 203/259 (78.38%), Postives = 223/259 (86.10%), Query Frame = 0

Query: 1   MGGESKSKILFIGSSGYIGKFVVEASAKAGHPTYVLLRDSILSDPSKSHIINTFKSLRVI 60
           M  +SK KILFIGS+GY+GKFVVEASAKAGHPTY LL DS LSDP+KSHIIN FKSL+VI
Sbjct: 4   MATQSKCKILFIGSTGYVGKFVVEASAKAGHPTYALLSDSTLSDPTKSHIINHFKSLKVI 63

Query: 61  FISGDLYDHESLVKAIKQVDVVISTVSHSQLAKQDRIVSAMKEAGNVKRFFPSEYGNDVD 120
           F+SG+LYDHESLVKAIKQVDVVISTV +   A Q RI+SA+KEAGNV+RF PSEYGNDVD
Sbjct: 64  FVSGNLYDHESLVKAIKQVDVVISTVGNDLFADQVRIISAIKEAGNVRRFLPSEYGNDVD 123

Query: 121 RVHGVEPAKSAFAAKAKIRRAVEVAKIPYTFVSSNFCSGWFLPSLAQPQP-QASTPPKDK 180
           RVHGVEPAKS F AKA+IR+AV+ A IP+TF+SSNFCS WFLPSLAQP    AS PPKDK
Sbjct: 124 RVHGVEPAKSLFNAKAQIRQAVKDANIPFTFISSNFCSAWFLPSLAQPYDLNASAPPKDK 183

Query: 181 VVILGDGNSK---GEEHDIGTYIIRAVDDPRTLNKILYVRPSQNTYSFIDLVSLWEKKCG 240
           V ILGDG SK    EEHDI TYII+AVDD RTLNKILYVRP QN YS++DLVSLWEKKCG
Sbjct: 184 VFILGDGISKVIFNEEHDIATYIIKAVDDSRTLNKILYVRPLQNIYSYVDLVSLWEKKCG 243

Query: 241 KILEKTYVTEEQLLKNIQG 256
            ILEK YV+EEQLLKNIQG
Sbjct: 244 IILEKIYVSEEQLLKNIQG 262

BLAST of Spg014302 vs. NCBI nr
Match: XP_008448084.2 (PREDICTED: isoflavone reductase-like protein isoform X1 [Cucumis melo])

HSP 1 Score: 393.7 bits (1010), Expect = 1.2e-105
Identity = 202/258 (78.29%), Postives = 222/258 (86.05%), Query Frame = 0

Query: 1   MGGESKSKILFIGSSGYIGKFVVEASAKAGHPTYVLLRDSILSDPSKSHIINTFKSLRVI 60
           M  +SK KILFIGS+GY+GKFVVEASAKAGHPTY LL DS LSDP+KSHIIN FKSL+VI
Sbjct: 4   MATQSKCKILFIGSTGYVGKFVVEASAKAGHPTYALLSDSTLSDPTKSHIINHFKSLKVI 63

Query: 61  FISGDLYDHESLVKAIKQVDVVISTVSHSQLAKQDRIVSAMKEAGNVKRFFPSEYGNDVD 120
           F+SG+LYDHESLVKAIKQVDVVISTV +   A Q RI+SA+KEAGNV+RF PSEYGNDVD
Sbjct: 64  FVSGNLYDHESLVKAIKQVDVVISTVGNDLFADQVRIISAIKEAGNVRRFLPSEYGNDVD 123

Query: 121 RVHGVEPAKSAFAAKAKIRRAVEVAKIPYTFVSSNFCSGWFLPSLAQPQP-QASTPPKDK 180
           RVHGVEPAKS F AKA+IR+AV+ A IP+TF+SSNFCS WFLPSLAQP    AS PPKDK
Sbjct: 124 RVHGVEPAKSLFNAKAQIRQAVKDANIPFTFISSNFCSAWFLPSLAQPYDLNASAPPKDK 183

Query: 181 VVILGDGNSK---GEEHDIGTYIIRAVDDPRTLNKILYVRPSQNTYSFIDLVSLWEKKCG 240
           V ILGDG SK    EEHDI TYII+AVDD RTLNKILYVRP QN YS++DLVSLWEKKCG
Sbjct: 184 VFILGDGISKVIFNEEHDIATYIIKAVDDSRTLNKILYVRPLQNIYSYVDLVSLWEKKCG 243

Query: 241 KILEKTYVTEEQLLKNIQ 255
            ILEK YV+EEQLLKNIQ
Sbjct: 244 IILEKIYVSEEQLLKNIQ 261

BLAST of Spg014302 vs. NCBI nr
Match: XP_022135658.1 (isoflavone reductase-like protein [Momordica charantia])

HSP 1 Score: 385.6 bits (989), Expect = 3.4e-103
Identity = 199/252 (78.97%), Postives = 219/252 (86.90%), Query Frame = 0

Query: 6   KSKILFIGSSGYIGKFVVEASAKAGHPTYVLLRDSILSDPSKSHIINTFKSLRVIFISGD 65
           KSKILFIGS+GY+GKFVVEASAKAGHPTYVLLRDS LS P+KS II  F+S  V F+SG+
Sbjct: 4   KSKILFIGSTGYVGKFVVEASAKAGHPTYVLLRDSTLSVPAKSQIIANFRSFGVNFLSGN 63

Query: 66  LYDHESLVKAIKQVDVVISTVSHSQLAKQDRIVSAMKEAGNVKRFFPSEYGNDVDRVHGV 125
           +YDHESLVKAIKQVDVVISTV H+QLA QDRIVSA+ EAGNVKRF PSEYGNDVDRVHGV
Sbjct: 64  MYDHESLVKAIKQVDVVISTVGHAQLADQDRIVSAIVEAGNVKRFLPSEYGNDVDRVHGV 123

Query: 126 EPAKSAFAAKAKIRRAVEVAKIPYTFVSSNFCSGWFLPSLAQPQPQASTPPKDKVVILGD 185
            PAKSAFAAKAKIRRAVE A+IP+TFVSSN  SGWFLPSLA  QP+AS+PP+DK VILGD
Sbjct: 124 APAKSAFAAKAKIRRAVEAAQIPFTFVSSNLFSGWFLPSLA--QPEASSPPRDKFVILGD 183

Query: 186 GNSK---GEEHDIGTYIIRAVDDPRTLNKILYVRPSQNTYSFIDLVSLWEKKCGKILEKT 245
           GN K    EEHDIGTYI+RAVDDPRTLNKILYVRP +N  SF DL SLW+KK GK L+K 
Sbjct: 184 GNPKAIFNEEHDIGTYIMRAVDDPRTLNKILYVRPPKNIISFKDLASLWDKKIGKTLQKI 243

Query: 246 YVTEEQLLKNIQ 255
           YV EEQ+LKNI+
Sbjct: 244 YVPEEQVLKNIE 253

BLAST of Spg014302 vs. ExPASy Swiss-Prot
Match: O81355 (Phenylcoumaran benzylic ether reductase Pyrc5 OS=Pyrus communis OX=23211 GN=PYRC5 PE=1 SV=1)

HSP 1 Score: 362.1 bits (928), Expect = 5.3e-99
Identity = 186/253 (73.52%), Postives = 213/253 (84.19%), Query Frame = 0

Query: 5   SKSKILFIGSSGYIGKFVVEASAKAGHPTYVLLRDSILSDPSKSHIINTFKSLRVIFISG 64
           SKS+ILFIG +GYIGKF+VEASAKAG+PTYVL+R++ LSDP+KS +I  FK+L V F+ G
Sbjct: 3   SKSQILFIGGTGYIGKFIVEASAKAGYPTYVLVREASLSDPAKSKVIENFKALGVNFVLG 62

Query: 65  DLYDHESLVKAIKQVDVVISTVSHSQLAKQDRIVSAMKEAGNVKRFFPSEYGNDVDRVHG 124
           DLYDHESLVKAIKQVDVVISTV H QLA Q +I++A+KEAGNVKRFFPSE+GNDVDR H 
Sbjct: 63  DLYDHESLVKAIKQVDVVISTVGHGQLADQGKIIAAIKEAGNVKRFFPSEFGNDVDRSHA 122

Query: 125 VEPAKSAFAAKAKIRRAVEVAKIPYTFVSSNFCSGWFLPSLAQPQPQASTPPKDKVVILG 184
           VEPAKSAF  KAKIRRAVE   IPYT+VSSNF +G+FLP+L   QP AS+ P+DKVVILG
Sbjct: 123 VEPAKSAFETKAKIRRAVEAEGIPYTYVSSNFFAGYFLPTL--NQPGASSAPRDKVVILG 182

Query: 185 DGNSK---GEEHDIGTYIIRAVDDPRTLNKILYVRPSQNTYSFIDLVSLWEKKCGKILEK 244
           DGN K    +E DIGTY IRAVDDPRTLNK+LY+RP  NT SF +LVSLWEKK GK LE+
Sbjct: 183 DGNPKAIFNKEDDIGTYTIRAVDDPRTLNKVLYIRPPANTISFNELVSLWEKKIGKTLER 242

Query: 245 TYVTEEQLLKNIQ 255
            YV EEQLLKNIQ
Sbjct: 243 IYVPEEQLLKNIQ 253

BLAST of Spg014302 vs. ExPASy Swiss-Prot
Match: B9HRL7 (Phenylcoumaran benzylic ether reductase POP1 OS=Populus trichocarpa OX=3694 GN=PCBER PE=1 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 3.8e-97
Identity = 182/251 (72.51%), Postives = 212/251 (84.46%), Query Frame = 0

Query: 7   SKILFIGSSGYIGKFVVEASAKAGHPTYVLLRDSILSDPSKSHIINTFKSLRVIFISGDL 66
           SKILFIG +GYIGKF+VEASAKAGHPT+VL+R+S LS+P+KS +I  FK+L V F+ GDL
Sbjct: 3   SKILFIGGTGYIGKFIVEASAKAGHPTFVLVRESTLSNPAKSVVIYNFKNLGVNFLIGDL 62

Query: 67  YDHESLVKAIKQVDVVISTVSHSQLAKQDRIVSAMKEAGNVKRFFPSEYGNDVDRVHGVE 126
           +DHESLVKAIKQVDVVISTV H+QL +QDRI++A+KEAGNVKRFFPSE+GNDVDRV+ VE
Sbjct: 63  FDHESLVKAIKQVDVVISTVGHAQLVEQDRIIAAIKEAGNVKRFFPSEFGNDVDRVNAVE 122

Query: 127 PAKSAFAAKAKIRRAVEVAKIPYTFVSSNFCSGWFLPSLAQPQPQASTPPKDKVVILGDG 186
           PAKSAFA KA +RRA+E   IPYT+VSSNF SG+FL  L+  QP A+ PP+DKVVILGDG
Sbjct: 123 PAKSAFATKANVRRAIEAEGIPYTYVSSNFFSGYFL--LSFNQPGATAPPRDKVVILGDG 182

Query: 187 NSK---GEEHDIGTYIIRAVDDPRTLNKILYVRPSQNTYSFIDLVSLWEKKCGKILEKTY 246
           N K    +E DI TY I+AVDDPRTLNKILY++P  NT SF DLVSLWEKK GK LE+ Y
Sbjct: 183 NPKAVFNKEDDIATYTIKAVDDPRTLNKILYIKPPANTISFNDLVSLWEKKIGKTLERIY 242

Query: 247 VTEEQLLKNIQ 255
           V EEQLLKNIQ
Sbjct: 243 VPEEQLLKNIQ 251

BLAST of Spg014302 vs. ExPASy Swiss-Prot
Match: Q9FUW6 (Phenylcoumaran benzylic ether reductase Betv6 OS=Betula pendula OX=3505 GN=BETV6 PE=1 SV=1)

HSP 1 Score: 342.4 bits (877), Expect = 4.3e-93
Identity = 169/252 (67.06%), Postives = 208/252 (82.54%), Query Frame = 0

Query: 6   KSKILFIGSSGYIGKFVVEASAKAGHPTYVLLRDSILSDPSKSHIINTFKSLRVIFISGD 65
           KSKIL IG +GYIGKF+VEASAK+GHPT+ L+R+S +SDP K  ++  FK L V  + GD
Sbjct: 4   KSKILIIGGTGYIGKFIVEASAKSGHPTFALVRESTVSDPVKGKLVEKFKGLGVTLLHGD 63

Query: 66  LYDHESLVKAIKQVDVVISTVSHSQLAKQDRIVSAMKEAGNVKRFFPSEYGNDVDRVHGV 125
           LYDHESLVKA KQVDVVISTV H QLA Q +I++A+KEAGN+KRFFPSE+GNDVDRVH V
Sbjct: 64  LYDHESLVKAFKQVDVVISTVGHLQLADQVKIIAAIKEAGNIKRFFPSEFGNDVDRVHAV 123

Query: 126 EPAKSAFAAKAKIRRAVEVAKIPYTFVSSNFCSGWFLPSLAQPQPQASTPPKDKVVILGD 185
           EPAK+AFA KA+IRR  E   IPYT+VSSNF +G+FLP+LA  QP  ++PP++KVVI GD
Sbjct: 124 EPAKTAFATKAEIRRKTEAEGIPYTYVSSNFFAGYFLPTLA--QPGLTSPPREKVVIFGD 183

Query: 186 GNSK---GEEHDIGTYIIRAVDDPRTLNKILYVRPSQNTYSFIDLVSLWEKKCGKILEKT 245
           GN++    +E DIGTY IRAVDDPRTLNKI+Y++P++N YSF ++V+LWEKK GK LEK 
Sbjct: 184 GNARAVFNKEDDIGTYTIRAVDDPRTLNKIVYIKPAKNIYSFNEIVALWEKKIGKTLEKI 243

Query: 246 YVTEEQLLKNIQ 255
           YV EE+LLK+IQ
Sbjct: 244 YVPEEKLLKDIQ 253

BLAST of Spg014302 vs. ExPASy Swiss-Prot
Match: Q9T030 (Phenylcoumaran benzylic ether reductase 1 OS=Arabidopsis thaliana OX=3702 GN=PCBER1 PE=1 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 5.3e-91
Identity = 172/252 (68.25%), Postives = 202/252 (80.16%), Query Frame = 0

Query: 5   SKSKILFIGSSGYIGKFVVEASAKAGHPTYVLLRDSILSDPSKSHIINTFKSLRVIFISG 64
           SKSKILFIG +GYIGK++VEASA++GHPT VL+R+S L+ PS+S  I  FK+L V F+ G
Sbjct: 3   SKSKILFIGGTGYIGKYIVEASARSGHPTLVLVRNSTLTSPSRSSTIENFKNLGVQFLLG 62

Query: 65  DLYDHESLVKAIKQVDVVISTVSHSQLAKQDRIVSAMKEAGNVKRFFPSEYGNDVDRVHG 124
           DL DH SLV +IKQ DVVISTV HS L  Q +I+SA+KEAGNVKRFFPSE+GNDVDRV  
Sbjct: 63  DLDDHTSLVNSIKQADVVISTVGHSLLGHQYKIISAIKEAGNVKRFFPSEFGNDVDRVFT 122

Query: 125 VEPAKSAFAAKAKIRRAVEVAKIPYTFVSSNFCSGWFLPSLAQPQPQASTPPKDKVVILG 184
           VEPAKSA+A KAKIRR +E   IPYT+VS NF +G+FLP+LA  QP A++ P+DKV++LG
Sbjct: 123 VEPAKSAYATKAKIRRTIEAEGIPYTYVSCNFFAGYFLPTLA--QPGATSAPRDKVIVLG 182

Query: 185 DGNSK---GEEHDIGTYIIRAVDDPRTLNKILYVRPSQNTYSFIDLVSLWEKKCGKILEK 244
           DGN K    +E DIGTY I AVDDPRTLNKILY+RP  NTYSF DLVSLWE K GK LE+
Sbjct: 183 DGNPKAVFNKEEDIGTYTINAVDDPRTLNKILYIRPPMNTYSFNDLVSLWENKIGKTLER 242

Query: 245 TYVTEEQLLKNI 254
            YV EEQLLK I
Sbjct: 243 IYVPEEQLLKQI 252

BLAST of Spg014302 vs. ExPASy Swiss-Prot
Match: P52578 (Isoflavone reductase homolog OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 1.2e-90
Identity = 170/252 (67.46%), Postives = 207/252 (82.14%), Query Frame = 0

Query: 6   KSKILFIGSSGYIGKFVVEASAKAGHPTYVLLRDSILSDPSKSHIINTFKSLRVIFISGD 65
           KSKILFIG +GYIGKF+VEASAKAGH T+VL+R+S LS+P+K+ +I+TFKS  V F+ GD
Sbjct: 4   KSKILFIGGTGYIGKFIVEASAKAGHDTFVLVRESTLSNPTKTKLIDTFKSFGVTFVHGD 63

Query: 66  LYDHESLVKAIKQVDVVISTVSHSQLAKQDRIVSAMKEAGNVKRFFPSEYGNDVDRVHGV 125
           LYDHESLVKAIKQVDVVISTV H+ LA Q ++++A+KEAGNVKRFFPSE+GNDVDRVH V
Sbjct: 64  LYDHESLVKAIKQVDVVISTVGHALLADQVKLIAAIKEAGNVKRFFPSEFGNDVDRVHAV 123

Query: 126 EPAKSAFAAKAKIRRAVEVAKIPYTFVSSNFCSGWFLPSLAQPQPQASTPPKDKVVILGD 185
           EPAK+AF  KA+IRR VE   IP+T+V++ F +G+ LP+LA  QP A+ PP DKVVILG 
Sbjct: 124 EPAKAAFNTKAQIRRVVEAEGIPFTYVATFFFAGYSLPNLA--QPGAAGPPNDKVVILGH 183

Query: 186 GNSK---GEEHDIGTYIIRAVDDPRTLNKILYVRPSQNTYSFIDLVSLWEKKCGKILEKT 245
           GN+K    +E DIGTY I AVDDP+TLNKILY++P  N  +  +LVSLWEKK GK LE+ 
Sbjct: 184 GNTKAVFNKEEDIGTYTINAVDDPKTLNKILYIKPPHNIITLNELVSLWEKKTGKNLERL 243

Query: 246 YVTEEQLLKNIQ 255
           YV EEQ+LKNIQ
Sbjct: 244 YVPEEQVLKNIQ 253

BLAST of Spg014302 vs. ExPASy TrEMBL
Match: A0A1S4DWU4 (isoflavone reductase-like protein isoform X3 OS=Cucumis melo OX=3656 GN=LOC103490369 PE=4 SV=1)

HSP 1 Score: 396.0 bits (1016), Expect = 1.2e-106
Identity = 203/259 (78.38%), Postives = 223/259 (86.10%), Query Frame = 0

Query: 1   MGGESKSKILFIGSSGYIGKFVVEASAKAGHPTYVLLRDSILSDPSKSHIINTFKSLRVI 60
           M  +SK KILFIGS+GY+GKFVVEASAKAGHPTY LL DS LSDP+KSHIIN FKSL+VI
Sbjct: 4   MATQSKCKILFIGSTGYVGKFVVEASAKAGHPTYALLSDSTLSDPTKSHIINHFKSLKVI 63

Query: 61  FISGDLYDHESLVKAIKQVDVVISTVSHSQLAKQDRIVSAMKEAGNVKRFFPSEYGNDVD 120
           F+SG+LYDHESLVKAIKQVDVVISTV +   A Q RI+SA+KEAGNV+RF PSEYGNDVD
Sbjct: 64  FVSGNLYDHESLVKAIKQVDVVISTVGNDLFADQVRIISAIKEAGNVRRFLPSEYGNDVD 123

Query: 121 RVHGVEPAKSAFAAKAKIRRAVEVAKIPYTFVSSNFCSGWFLPSLAQPQP-QASTPPKDK 180
           RVHGVEPAKS F AKA+IR+AV+ A IP+TF+SSNFCS WFLPSLAQP    AS PPKDK
Sbjct: 124 RVHGVEPAKSLFNAKAQIRQAVKDANIPFTFISSNFCSAWFLPSLAQPYDLNASAPPKDK 183

Query: 181 VVILGDGNSK---GEEHDIGTYIIRAVDDPRTLNKILYVRPSQNTYSFIDLVSLWEKKCG 240
           V ILGDG SK    EEHDI TYII+AVDD RTLNKILYVRP QN YS++DLVSLWEKKCG
Sbjct: 184 VFILGDGISKVIFNEEHDIATYIIKAVDDSRTLNKILYVRPLQNIYSYVDLVSLWEKKCG 243

Query: 241 KILEKTYVTEEQLLKNIQG 256
            ILEK YV+EEQLLKNIQG
Sbjct: 244 IILEKIYVSEEQLLKNIQG 262

BLAST of Spg014302 vs. ExPASy TrEMBL
Match: A0A1S4DXK7 (isoflavone reductase homolog isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490369 PE=4 SV=1)

HSP 1 Score: 396.0 bits (1016), Expect = 1.2e-106
Identity = 203/259 (78.38%), Postives = 223/259 (86.10%), Query Frame = 0

Query: 1   MGGESKSKILFIGSSGYIGKFVVEASAKAGHPTYVLLRDSILSDPSKSHIINTFKSLRVI 60
           M  +SK KILFIGS+GY+GKFVVEASAKAGHPTY LL DS LSDP+KSHIIN FKSL+VI
Sbjct: 4   MATQSKCKILFIGSTGYVGKFVVEASAKAGHPTYALLSDSTLSDPTKSHIINHFKSLKVI 63

Query: 61  FISGDLYDHESLVKAIKQVDVVISTVSHSQLAKQDRIVSAMKEAGNVKRFFPSEYGNDVD 120
           F+SG+LYDHESLVKAIKQVDVVISTV +   A Q RI+SA+KEAGNV+RF PSEYGNDVD
Sbjct: 64  FVSGNLYDHESLVKAIKQVDVVISTVGNDLFADQVRIISAIKEAGNVRRFLPSEYGNDVD 123

Query: 121 RVHGVEPAKSAFAAKAKIRRAVEVAKIPYTFVSSNFCSGWFLPSLAQPQP-QASTPPKDK 180
           RVHGVEPAKS F AKA+IR+AV+ A IP+TF+SSNFCS WFLPSLAQP    AS PPKDK
Sbjct: 124 RVHGVEPAKSLFNAKAQIRQAVKDANIPFTFISSNFCSAWFLPSLAQPYDLNASAPPKDK 183

Query: 181 VVILGDGNSK---GEEHDIGTYIIRAVDDPRTLNKILYVRPSQNTYSFIDLVSLWEKKCG 240
           V ILGDG SK    EEHDI TYII+AVDD RTLNKILYVRP QN YS++DLVSLWEKKCG
Sbjct: 184 VFILGDGISKVIFNEEHDIATYIIKAVDDSRTLNKILYVRPLQNIYSYVDLVSLWEKKCG 243

Query: 241 KILEKTYVTEEQLLKNIQG 256
            ILEK YV+EEQLLKNIQG
Sbjct: 244 IILEKIYVSEEQLLKNIQG 262

BLAST of Spg014302 vs. ExPASy TrEMBL
Match: A0A1S3BIV9 (isoflavone reductase-like protein isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490369 PE=4 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 6.0e-106
Identity = 202/258 (78.29%), Postives = 222/258 (86.05%), Query Frame = 0

Query: 1   MGGESKSKILFIGSSGYIGKFVVEASAKAGHPTYVLLRDSILSDPSKSHIINTFKSLRVI 60
           M  +SK KILFIGS+GY+GKFVVEASAKAGHPTY LL DS LSDP+KSHIIN FKSL+VI
Sbjct: 4   MATQSKCKILFIGSTGYVGKFVVEASAKAGHPTYALLSDSTLSDPTKSHIINHFKSLKVI 63

Query: 61  FISGDLYDHESLVKAIKQVDVVISTVSHSQLAKQDRIVSAMKEAGNVKRFFPSEYGNDVD 120
           F+SG+LYDHESLVKAIKQVDVVISTV +   A Q RI+SA+KEAGNV+RF PSEYGNDVD
Sbjct: 64  FVSGNLYDHESLVKAIKQVDVVISTVGNDLFADQVRIISAIKEAGNVRRFLPSEYGNDVD 123

Query: 121 RVHGVEPAKSAFAAKAKIRRAVEVAKIPYTFVSSNFCSGWFLPSLAQPQP-QASTPPKDK 180
           RVHGVEPAKS F AKA+IR+AV+ A IP+TF+SSNFCS WFLPSLAQP    AS PPKDK
Sbjct: 124 RVHGVEPAKSLFNAKAQIRQAVKDANIPFTFISSNFCSAWFLPSLAQPYDLNASAPPKDK 183

Query: 181 VVILGDGNSK---GEEHDIGTYIIRAVDDPRTLNKILYVRPSQNTYSFIDLVSLWEKKCG 240
           V ILGDG SK    EEHDI TYII+AVDD RTLNKILYVRP QN YS++DLVSLWEKKCG
Sbjct: 184 VFILGDGISKVIFNEEHDIATYIIKAVDDSRTLNKILYVRPLQNIYSYVDLVSLWEKKCG 243

Query: 241 KILEKTYVTEEQLLKNIQ 255
            ILEK YV+EEQLLKNIQ
Sbjct: 244 IILEKIYVSEEQLLKNIQ 261

BLAST of Spg014302 vs. ExPASy TrEMBL
Match: A0A6J1C5E9 (isoflavone reductase-like protein OS=Momordica charantia OX=3673 GN=LOC111007562 PE=4 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 1.6e-103
Identity = 199/252 (78.97%), Postives = 219/252 (86.90%), Query Frame = 0

Query: 6   KSKILFIGSSGYIGKFVVEASAKAGHPTYVLLRDSILSDPSKSHIINTFKSLRVIFISGD 65
           KSKILFIGS+GY+GKFVVEASAKAGHPTYVLLRDS LS P+KS II  F+S  V F+SG+
Sbjct: 4   KSKILFIGSTGYVGKFVVEASAKAGHPTYVLLRDSTLSVPAKSQIIANFRSFGVNFLSGN 63

Query: 66  LYDHESLVKAIKQVDVVISTVSHSQLAKQDRIVSAMKEAGNVKRFFPSEYGNDVDRVHGV 125
           +YDHESLVKAIKQVDVVISTV H+QLA QDRIVSA+ EAGNVKRF PSEYGNDVDRVHGV
Sbjct: 64  MYDHESLVKAIKQVDVVISTVGHAQLADQDRIVSAIVEAGNVKRFLPSEYGNDVDRVHGV 123

Query: 126 EPAKSAFAAKAKIRRAVEVAKIPYTFVSSNFCSGWFLPSLAQPQPQASTPPKDKVVILGD 185
            PAKSAFAAKAKIRRAVE A+IP+TFVSSN  SGWFLPSLA  QP+AS+PP+DK VILGD
Sbjct: 124 APAKSAFAAKAKIRRAVEAAQIPFTFVSSNLFSGWFLPSLA--QPEASSPPRDKFVILGD 183

Query: 186 GNSK---GEEHDIGTYIIRAVDDPRTLNKILYVRPSQNTYSFIDLVSLWEKKCGKILEKT 245
           GN K    EEHDIGTYI+RAVDDPRTLNKILYVRP +N  SF DL SLW+KK GK L+K 
Sbjct: 184 GNPKAIFNEEHDIGTYIMRAVDDPRTLNKILYVRPPKNIISFKDLASLWDKKIGKTLQKI 243

Query: 246 YVTEEQLLKNIQ 255
           YV EEQ+LKNI+
Sbjct: 244 YVPEEQVLKNIE 253

BLAST of Spg014302 vs. ExPASy TrEMBL
Match: A0A6J1GNQ2 (isoflavone reductase-like protein isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111455600 PE=4 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 3.1e-102
Identity = 192/254 (75.59%), Postives = 222/254 (87.40%), Query Frame = 0

Query: 5   SKSKILFIGSSGYIGKFVVEASAKAGHPTYVLLRDSILSDPSKSHIINTFKSLRVIFISG 64
           +KSKIL IG +GYIGKF+VEASA AGHPT+VL+R S LSDP+KS +IN F +L VIF+SG
Sbjct: 3   AKSKILIIGGTGYIGKFIVEASAMAGHPTHVLVRGSTLSDPAKSELINHFNTLGVIFVSG 62

Query: 65  DLYDHESLVKAIKQVDVVISTVSHSQLAKQDRIVSAMKEAGNVKRFFPSEYGNDVDRVHG 124
           DL+ HESLVKAIKQVDVVIST+ HS L  QDRI+SA+KEAGNVKRFFPSEYGNDVDRVHG
Sbjct: 63  DLFVHESLVKAIKQVDVVISTIGHSLLPDQDRIISAIKEAGNVKRFFPSEYGNDVDRVHG 122

Query: 125 VEPAKSAFAAKAKIRRAVEVAKIPYTFVSSNFCSGWFLPSLAQPQ-PQASTPPKDKVVIL 184
           +EPA++AF AKA IRRAVE A+IPYT+VSSNFCSGWFLPSLAQP   +A+ PPKDKVVIL
Sbjct: 123 IEPARTAFNAKAGIRRAVEAAQIPYTYVSSNFCSGWFLPSLAQPYLLEAAAPPKDKVVIL 182

Query: 185 GDGNSK---GEEHDIGTYIIRAVDDPRTLNKILYVRPSQNTYSFIDLVSLWEKKCGKILE 244
           GDGN K     E DI TYII+AVDDPRTLNKILYVRP QNTYS++DLV+LWE+K GKIL+
Sbjct: 183 GDGNFKAIFNVERDIATYIIKAVDDPRTLNKILYVRPLQNTYSYVDLVALWEQKYGKILD 242

Query: 245 KTYVTEEQLLKNIQ 255
           K+YV+E+QLL +IQ
Sbjct: 243 KSYVSEQQLLNDIQ 256

BLAST of Spg014302 vs. TAIR 10
Match: AT4G39230.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 335.5 bits (859), Expect = 3.7e-92
Identity = 172/252 (68.25%), Postives = 202/252 (80.16%), Query Frame = 0

Query: 5   SKSKILFIGSSGYIGKFVVEASAKAGHPTYVLLRDSILSDPSKSHIINTFKSLRVIFISG 64
           SKSKILFIG +GYIGK++VEASA++GHPT VL+R+S L+ PS+S  I  FK+L V F+ G
Sbjct: 3   SKSKILFIGGTGYIGKYIVEASARSGHPTLVLVRNSTLTSPSRSSTIENFKNLGVQFLLG 62

Query: 65  DLYDHESLVKAIKQVDVVISTVSHSQLAKQDRIVSAMKEAGNVKRFFPSEYGNDVDRVHG 124
           DL DH SLV +IKQ DVVISTV HS L  Q +I+SA+KEAGNVKRFFPSE+GNDVDRV  
Sbjct: 63  DLDDHTSLVNSIKQADVVISTVGHSLLGHQYKIISAIKEAGNVKRFFPSEFGNDVDRVFT 122

Query: 125 VEPAKSAFAAKAKIRRAVEVAKIPYTFVSSNFCSGWFLPSLAQPQPQASTPPKDKVVILG 184
           VEPAKSA+A KAKIRR +E   IPYT+VS NF +G+FLP+LA  QP A++ P+DKV++LG
Sbjct: 123 VEPAKSAYATKAKIRRTIEAEGIPYTYVSCNFFAGYFLPTLA--QPGATSAPRDKVIVLG 182

Query: 185 DGNSK---GEEHDIGTYIIRAVDDPRTLNKILYVRPSQNTYSFIDLVSLWEKKCGKILEK 244
           DGN K    +E DIGTY I AVDDPRTLNKILY+RP  NTYSF DLVSLWE K GK LE+
Sbjct: 183 DGNPKAVFNKEEDIGTYTINAVDDPRTLNKILYIRPPMNTYSFNDLVSLWENKIGKTLER 242

Query: 245 TYVTEEQLLKNI 254
            YV EEQLLK I
Sbjct: 243 IYVPEEQLLKQI 252

BLAST of Spg014302 vs. TAIR 10
Match: AT1G75280.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 310.8 bits (795), Expect = 9.9e-85
Identity = 157/252 (62.30%), Postives = 193/252 (76.59%), Query Frame = 0

Query: 6   KSKILFIGSSGYIGKFVVEASAKAGHPTYVLLRDSILSDPSKSHIINTFKSLRVIFISGD 65
           KSKIL IG +GYIGKF+VEASAKAGH T+ L+R++ LSDP K   + +FK L V  + GD
Sbjct: 5   KSKILVIGGTGYIGKFLVEASAKAGHSTFALVREATLSDPVKGKTVQSFKDLGVTILHGD 64

Query: 66  LYDHESLVKAIKQVDVVISTVSHSQLAKQDRIVSAMKEAGNVKRFFPSEYGNDVDRVHGV 125
           L DHESLVKAIKQVDVVISTV   Q+  Q +I+SA+KEAGNVKRF PSE+G DVDR   V
Sbjct: 65  LNDHESLVKAIKQVDVVISTVGSMQILDQTKIISAIKEAGNVKRFLPSEFGVDVDRTSAV 124

Query: 126 EPAKSAFAAKAKIRRAVEVAKIPYTFVSSNFCSGWFLPSLAQPQPQASTPPKDKVVILGD 185
           EPAKSAFA K +IRR +E   IPYT+  +    G++LP+L Q +P  ++PP+DKV ILGD
Sbjct: 125 EPAKSAFAGKIQIRRTIEAEGIPYTYAVTGCFGGYYLPTLVQFEPGLTSPPRDKVTILGD 184

Query: 186 GNSK---GEEHDIGTYIIRAVDDPRTLNKILYVRPSQNTYSFIDLVSLWEKKCGKILEKT 245
           GN+K    +E DI  Y I+AVDDPRTLNKILY++PS NT S  ++V+LWEKK GK LEKT
Sbjct: 185 GNAKAVINKEEDIAAYTIKAVDDPRTLNKILYIKPSNNTLSMNEIVTLWEKKIGKSLEKT 244

Query: 246 YVTEEQLLKNIQ 255
           ++ EEQLLK+IQ
Sbjct: 245 HLPEEQLLKSIQ 256

BLAST of Spg014302 vs. TAIR 10
Match: AT1G75290.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 289.3 bits (739), Expect = 3.1e-78
Identity = 143/252 (56.75%), Postives = 184/252 (73.02%), Query Frame = 0

Query: 6   KSKILFIGSSGYIGKFVVEASAKAGHPTYVLLRDSILSDPSKSHIINTFKSLRVIFISGD 65
           KSKIL IG +G+IGK ++EAS KAGH T  L+R++ LSDP+K   +  FK   V  + GD
Sbjct: 5   KSKILVIGGTGHIGKLIIEASVKAGHSTLALVREASLSDPNKGKTVQNFKDFGVTLLHGD 64

Query: 66  LYDHESLVKAIKQVDVVISTVSHSQLAKQDRIVSAMKEAGNVKRFFPSEYGNDVDRVHGV 125
           L DHESLVKAIKQ DVVISTV   Q+  Q +I+SA+KEAGNVKRF PSE+G DVD+   V
Sbjct: 65  LNDHESLVKAIKQADVVISTVGSMQILDQTKIISAIKEAGNVKRFLPSEFGMDVDKSSAV 124

Query: 126 EPAKSAFAAKAKIRRAVEVAKIPYTFVSSNFCSGWFLPSLAQPQPQASTPPKDKVVILGD 185
           EPAKSAF  K + RR +E   IPYT++ +N+ +G++LP+L Q +P  ++PP+DKV I GD
Sbjct: 125 EPAKSAFGRKLQTRRDIEAEGIPYTYLVTNYFAGYYLPTLVQLEPGLTSPPRDKVKIFGD 184

Query: 186 GNSK---GEEHDIGTYIIRAVDDPRTLNKILYVRPSQNTYSFIDLVSLWEKKCGKILEKT 245
           GN K    +E DI  Y I+AVDDPRTLNK LY+ P  NT S  ++V+LWEKK GK +EK 
Sbjct: 185 GNVKAVINKEEDIAAYTIKAVDDPRTLNKTLYINPPNNTLSMNEIVTLWEKKIGKSVEKI 244

Query: 246 YVTEEQLLKNIQ 255
           Y++EEQ+ K+IQ
Sbjct: 245 YMSEEQIFKSIQ 256

BLAST of Spg014302 vs. TAIR 10
Match: AT1G75300.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 288.9 bits (738), Expect = 4.0e-78
Identity = 149/266 (56.02%), Postives = 192/266 (72.18%), Query Frame = 0

Query: 6   KSKILFIGSSGYIGKFVVEASAKAGHPTYVLLRDSILSDPSKSHIINTFKSLRVIFISGD 65
           KSKIL IG +GY+G+F+VE SAKAG+PT+ L+R++ LSDP KS  I +FK L V  + GD
Sbjct: 5   KSKILVIGGTGYMGEFIVEGSAKAGNPTFALVREASLSDPVKSKTIQSFKDLGVTILHGD 64

Query: 66  LYDHESLVKAIKQVDVVISTVSHSQLAKQDRIVSAMKEAGNVKRFFPSEYGNDVDRVHGV 125
           L DHESLVKAIKQVDVVIST+ H Q+  Q +I+SA+KEAGNVKRF P+E+G DV+R   V
Sbjct: 65  LNDHESLVKAIKQVDVVISTIGHKQIFDQTKIISAIKEAGNVKRFLPAEFGIDVERTSAV 124

Query: 126 EPAKSAFAAKAKIRRAVEVAKIPYTFVSSNFCSGWFLPSLAQPQP--------------Q 185
           EPAKS FA K +IRRA+E   IPYT+V SN  +G++L +L Q +                
Sbjct: 125 EPAKSLFAGKVQIRRAIEAEGIPYTYVVSNCSAGFYLRTLLQFESGLISHTRDKAIIFGD 184

Query: 186 ASTPPKDKVVILGDGNSK---GEEHDIGTYIIRAVDDPRTLNKILYVRPSQNTYSFIDLV 245
            + PP+DKV ILGDGN+K    +E D+  Y+I+AVDD RTLNK LY+ P  N  S  ++V
Sbjct: 185 KNVPPRDKVTILGDGNAKVVINKEEDVAAYMIKAVDDLRTLNKTLYISPPNNILSMNEMV 244

Query: 246 SLWEKKCGKILEKTYVTEEQLLKNIQ 255
           +LWEKK GK LEKT+++EEQ+LK+IQ
Sbjct: 245 TLWEKKIGKSLEKTHISEEQILKSIQ 270

BLAST of Spg014302 vs. TAIR 10
Match: AT1G19540.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 266.2 bits (679), Expect = 2.8e-71
Identity = 141/253 (55.73%), Postives = 176/253 (69.57%), Query Frame = 0

Query: 7   SKILFIGSSGYIGKFVVEASAKAGHPTYVLLRDSILSDPSKSHIINTFKSLRVIFISGDL 66
           SKIL IG++G IGK +VE SAK+GH T+ L+R++ LSDP K+ ++  FK L V  + G L
Sbjct: 3   SKILVIGATGLIGKVLVEESAKSGHATFALVREASLSDPVKAQLVERFKDLGVTILYGSL 62

Query: 67  YDHESLVKAIKQVDVVISTVS--HSQLAKQDRIVSAMKEAGNVKRFFPSEYGNDVDRVHG 126
            D ESLVKAIKQVDVVIS V    +++  Q  I+ A+KE+GNVKRF PSE+GNDVDR   
Sbjct: 63  SDKESLVKAIKQVDVVISAVGRFQTEILNQTNIIDAIKESGNVKRFLPSEFGNDVDRTVA 122

Query: 127 VEPAKSAFAAKAKIRRAVEVAKIPYTFVSSNFCSGWFLPSLAQPQPQASTPPKDKVVILG 186
           +EP  S F  KA+IRRA+E AKIPYT+V S   +G F+P L Q   +  +PP+DKV I  
Sbjct: 123 IEPTLSEFITKAQIRRAIEAAKIPYTYVVSGCFAGLFVPCLGQCHLRLRSPPRDKVSIYD 182

Query: 187 DGNSK---GEEHDIGTYIIRAVDDPRTLNKILYVRPSQNTYSFIDLVSLWEKKCGKILEK 246
            GN K     E DI  Y ++AVDDPRTLNKILY+ P     S  D+V LWE+K GK LEK
Sbjct: 183 TGNGKAIVNTEEDIVAYTLKAVDDPRTLNKILYIHPPNYIVSQNDMVGLWEEKIGKTLEK 242

Query: 247 TYVTEEQLLKNIQ 255
           TYV+EE+LLK IQ
Sbjct: 243 TYVSEEELLKTIQ 255

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038888446.12.3e-11281.32phenylcoumaran benzylic ether reductase POP1-like [Benincasa hispida][more]
XP_016900450.12.5e-10678.38PREDICTED: isoflavone reductase homolog isoform X2 [Cucumis melo][more]
XP_016900451.12.5e-10678.38PREDICTED: isoflavone reductase-like protein isoform X3 [Cucumis melo][more]
XP_008448084.21.2e-10578.29PREDICTED: isoflavone reductase-like protein isoform X1 [Cucumis melo][more]
XP_022135658.13.4e-10378.97isoflavone reductase-like protein [Momordica charantia][more]
Match NameE-valueIdentityDescription
O813555.3e-9973.52Phenylcoumaran benzylic ether reductase Pyrc5 OS=Pyrus communis OX=23211 GN=PYRC... [more]
B9HRL73.8e-9772.51Phenylcoumaran benzylic ether reductase POP1 OS=Populus trichocarpa OX=3694 GN=P... [more]
Q9FUW64.3e-9367.06Phenylcoumaran benzylic ether reductase Betv6 OS=Betula pendula OX=3505 GN=BETV6... [more]
Q9T0305.3e-9168.25Phenylcoumaran benzylic ether reductase 1 OS=Arabidopsis thaliana OX=3702 GN=PCB... [more]
P525781.2e-9067.46Isoflavone reductase homolog OS=Solanum tuberosum OX=4113 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S4DWU41.2e-10678.38isoflavone reductase-like protein isoform X3 OS=Cucumis melo OX=3656 GN=LOC10349... [more]
A0A1S4DXK71.2e-10678.38isoflavone reductase homolog isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490369 ... [more]
A0A1S3BIV96.0e-10678.29isoflavone reductase-like protein isoform X1 OS=Cucumis melo OX=3656 GN=LOC10349... [more]
A0A6J1C5E91.6e-10378.97isoflavone reductase-like protein OS=Momordica charantia OX=3673 GN=LOC111007562... [more]
A0A6J1GNQ23.1e-10275.59isoflavone reductase-like protein isoform X2 OS=Cucurbita moschata OX=3662 GN=LO... [more]
Match NameE-valueIdentityDescription
AT4G39230.13.7e-9268.25NmrA-like negative transcriptional regulator family protein [more]
AT1G75280.19.9e-8562.30NmrA-like negative transcriptional regulator family protein [more]
AT1G75290.13.1e-7856.75NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G75300.14.0e-7856.02NmrA-like negative transcriptional regulator family protein [more]
AT1G19540.12.8e-7155.73NmrA-like negative transcriptional regulator family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008030NmrA-like domainPFAMPF05368NmrAcoord: 9..237
e-value: 2.0E-71
score: 240.2
NoneNo IPR availableGENE3D3.40.50.720coord: 8..216
e-value: 1.7E-103
score: 347.8
NoneNo IPR availableGENE3D3.90.25.10coord: 156..254
e-value: 1.7E-103
score: 347.8
NoneNo IPR availablePANTHERPTHR43349:SF35PHENYLCOUMARAN BENZYLIC ETHER REDUCTASE 1coord: 5..254
NoneNo IPR availablePANTHERPTHR43349PINORESINOL REDUCTASE-RELATEDcoord: 5..254
NoneNo IPR availableCDDcd05259PCBER_SDR_acoord: 8..254
e-value: 2.34939E-67
score: 208.311
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 5..253

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg014302.1Spg014302.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009807 lignan biosynthetic process
molecular_function GO:0050664 oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor