Spg014170 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg014170
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	lactoylglutathione lyase
Location	scaffold3: 45916807 .. 45917865 (-)
RNA-Seq Expression	Spg014170
Synteny	Spg014170

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGAAAAACCTTTAAAAATCCATTTCCCAATGAAACAAAAGCAATCAATCAGCCGTACGTCTTCTCCGCATAAACTTCCACGTACAGCTCCATCTTCTTCCATGGCAGTTGGCACTCTCCTTCCTTCCCCACGTGTGCCCTATCCGCCGCCATTGTTGCCTCTTTTAAACCAACCACCCTCCACACTCTTCTTCCACTGCTCTGCTTCGATTCGACGCCAATCGCGAACACACATGGAAGAACAGATTGCAGATCGGAAGGCCATCGACGAGGAAGTCCAGACGAACAACAACGAATTAGAAGAATCACAAGAAATTCGTGAGAGAGACAGTACAAGAAAGGAGGAAGAACATCCACTTCTACTAATGGCGCTCAATCACGTCTCCAGAGTGTGCAGAAGCGTCAAGGATTCCGTCGATTTCTACACCAAGGTCCTAGGGTTCGTCCTGATCGAGAGGCCGCAGAGCTTCGATTTCGAAGGGGCTTGGTTGTTCAACTACGGCGTCGGGATTCACTTGATGCAATCTGAGGATGAAGATAATGGAGTTCGTCCAGGCGGCGATAAGGATCATCTCGATCCCTTGGACAATCACATCTCGTTTCAGGTCAGTTTCTGAAACAAAATCGAAAGAAAATTTTGGTTACATTTTTTAGGTTTTGTTTTGTTGAAATTGAGCTGTGGCAGTGCGAAGATATGGAAGCGATGGAGGAGAAACTGAAGGAATTGGGAGTGAAATACATGAGGAGGACATTGGAAGAGGAGAAGGGAGAATCGATTGAACAGCTTTTCTTCAACGATCCTGACGGATTCATGATCGAGATCTGCAACTGCGAGAATCTCAAGGTGGTTCCAGCGGGTTCCTCCGGCAGGATTAGGCTTCCGGCCGGTCGCCATAACCCTCCATTGGAATCGAAAGAATAGAAATAGTTTCTCCAAGGTCGTTTTACTTATTTTGTAATTCATAAGCATATCCTGTAAAATCTAAAATAATAGTTCCACATTTTTTCTGGGACTTCTTTTTTTCTTCATTCGTTTAAATGAAATCCTGGTAAATATACC

mRNA sequence

ATGAAACAAAAGCAATCAATCAGCCGTACGTCTTCTCCGCATAAACTTCCACGTACAGCTCCATCTTCTTCCATGGCAGTTGGCACTCTCCTTCCTTCCCCACGTGTGCCCTATCCGCCGCCATTGTTGCCTCTTTTAAACCAACCACCCTCCACACTCTTCTTCCACTGCTCTGCTTCGATTCGACGCCAATCGCGAACACACATGGAAGAACAGATTGCAGATCGGAAGGCCATCGACGAGGAAGTCCAGACGAACAACAACGAATTAGAAGAATCACAAGAAATTCGTGAGAGAGACAGTACAAGAAAGGAGGAAGAACATCCACTTCTACTAATGGCGCTCAATCACGTCTCCAGAGTGTGCAGAAGCGTCAAGGATTCCGTCGATTTCTACACCAAGGTCCTAGGGTTCGTCCTGATCGAGAGGCCGCAGAGCTTCGATTTCGAAGGGGCTTGGTTGTTCAACTACGGCGTCGGGATTCACTTGATGCAATCTGAGGATGAAGATAATGGAGTTCGTCCAGGCGGCGATAAGGATCATCTCGATCCCTTGGACAATCACATCTCGTTTCAGTGCGAAGATATGGAAGCGATGGAGGAGAAACTGAAGGAATTGGGAGTGAAATACATGAGGAGGACATTGGAAGAGGAGAAGGGAGAATCGATTGAACAGCTTTTCTTCAACGATCCTGACGGATTCATGATCGAGATCTGCAACTGCGAGAATCTCAAGGTGGTTCCAGCGGGTTCCTCCGGCAGGATTAGGCTTCCGGCCGGTCGCCATAACCCTCCATTGGAATCGAAAGAATAG

Coding sequence (CDS)

ATGAAACAAAAGCAATCAATCAGCCGTACGTCTTCTCCGCATAAACTTCCACGTACAGCTCCATCTTCTTCCATGGCAGTTGGCACTCTCCTTCCTTCCCCACGTGTGCCCTATCCGCCGCCATTGTTGCCTCTTTTAAACCAACCACCCTCCACACTCTTCTTCCACTGCTCTGCTTCGATTCGACGCCAATCGCGAACACACATGGAAGAACAGATTGCAGATCGGAAGGCCATCGACGAGGAAGTCCAGACGAACAACAACGAATTAGAAGAATCACAAGAAATTCGTGAGAGAGACAGTACAAGAAAGGAGGAAGAACATCCACTTCTACTAATGGCGCTCAATCACGTCTCCAGAGTGTGCAGAAGCGTCAAGGATTCCGTCGATTTCTACACCAAGGTCCTAGGGTTCGTCCTGATCGAGAGGCCGCAGAGCTTCGATTTCGAAGGGGCTTGGTTGTTCAACTACGGCGTCGGGATTCACTTGATGCAATCTGAGGATGAAGATAATGGAGTTCGTCCAGGCGGCGATAAGGATCATCTCGATCCCTTGGACAATCACATCTCGTTTCAGTGCGAAGATATGGAAGCGATGGAGGAGAAACTGAAGGAATTGGGAGTGAAATACATGAGGAGGACATTGGAAGAGGAGAAGGGAGAATCGATTGAACAGCTTTTCTTCAACGATCCTGACGGATTCATGATCGAGATCTGCAACTGCGAGAATCTCAAGGTGGTTCCAGCGGGTTCCTCCGGCAGGATTAGGCTTCCGGCCGGTCGCCATAACCCTCCATTGGAATCGAAAGAATAG

Protein sequence

MKQKQSISRTSSPHKLPRTAPSSSMAVGTLLPSPRVPYPPPLLPLLNQPPSTLFFHCSASIRRQSRTHMEEQIADRKAIDEEVQTNNNELEESQEIRERDSTRKEEEHPLLLMALNHVSRVCRSVKDSVDFYTKVLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDNGVRPGGDKDHLDPLDNHISFQCEDMEAMEEKLKELGVKYMRRTLEEEKGESIEQLFFNDPDGFMIEICNCENLKVVPAGSSGRIRLPAGRHNPPLESKE

Homology

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BLAST of Spg014170 vs. NCBI nr
Match: XP_023548789.1 (lactoylglutathione lyase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 352.4 bits (903), Expect = 3.4e-93
Identity = 180/201 (89.55%), Postives = 188/201 (93.53%), Query Frame = 0


Query: 69  MEEQIADRKA-IDEEVQTNNNELEESQEIRERDSTRKEEEHPLLLMALNHVSRVCRSVKD 128
           ME++IADRKA +D EVQ  N+   ES+EI ER+STRKEEEHPL LMALNHVSRVC SVKD
Sbjct: 1   MEKEIADRKATVDNEVQEKND--GESEEITERESTRKEEEHPLPLMALNHVSRVCSSVKD 60

Query: 129 SVDFYTKVLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDNGVRPGGDKDHLDPLDN 188
           SVDFYTKVLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDNGVR  GDKDHLDPLDN
Sbjct: 61  SVDFYTKVLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDNGVRQ-GDKDHLDPLDN 120

Query: 189 HISFQCEDMEAMEEKLKELGVKYMRRTLEEEKGESIEQLFFNDPDGFMIEICNCENLKVV 248
           HISFQCEDMEAMEE+LKELGVKYMRRTL+EE GESIEQLFFNDPDGFMIEICNCENLKVV
Sbjct: 121 HISFQCEDMEAMEERLKELGVKYMRRTLDEENGESIEQLFFNDPDGFMIEICNCENLKVV 180

Query: 249 PAGSSGRIRLPAGRHNPPLES 269
           PAGSSGRIRLPAGRHNPPLES
Sbjct: 181 PAGSSGRIRLPAGRHNPPLES 198

[Close]

BLAST of Spg014170 vs. NCBI nr
Match: KAG6575712.1 (Lactoylglutathione lyase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 351.7 bits (901), Expect = 5.7e-93
Identity = 179/201 (89.05%), Postives = 188/201 (93.53%), Query Frame = 0


Query: 69  MEEQIADRKA-IDEEVQTNNNELEESQEIRERDSTRKEEEHPLLLMALNHVSRVCRSVKD 128
           ME++IADRKA +  EVQ N+    ES+EI ER++TRKEEEHPL LMALNHVSRVCRSVKD
Sbjct: 1   MEKEIADRKATVGNEVQENDG---ESEEITERENTRKEEEHPLPLMALNHVSRVCRSVKD 60

Query: 129 SVDFYTKVLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDNGVRPGGDKDHLDPLDN 188
           SVDFYTKVLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDNGVR  GDKDHLDPLDN
Sbjct: 61  SVDFYTKVLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDNGVRQ-GDKDHLDPLDN 120

Query: 189 HISFQCEDMEAMEEKLKELGVKYMRRTLEEEKGESIEQLFFNDPDGFMIEICNCENLKVV 248
           HISFQCEDMEAMEE+LKELGVKYMRRTL+EE GESIEQLFFNDPDGFMIEICNCENLKVV
Sbjct: 121 HISFQCEDMEAMEERLKELGVKYMRRTLDEENGESIEQLFFNDPDGFMIEICNCENLKVV 180

Query: 249 PAGSSGRIRLPAGRHNPPLES 269
           PAGSSGRIRLPAGRHNPPLES
Sbjct: 181 PAGSSGRIRLPAGRHNPPLES 197

[Close]

BLAST of Spg014170 vs. NCBI nr
Match: XP_022953340.1 (lactoylglutathione lyase [Cucurbita moschata])

HSP 1 Score: 351.3 bits (900), Expect = 7.5e-93
Identity = 180/201 (89.55%), Postives = 188/201 (93.53%), Query Frame = 0


Query: 69  MEEQIADRKA-IDEEVQTNNNELEESQEIRERDSTRKEEEHPLLLMALNHVSRVCRSVKD 128
           ME++IADRKA +  EVQ NN+   ES+EI ER+STRKEEEHPL LMALNHVSRVCRSVKD
Sbjct: 1   MEKEIADRKATVINEVQENND--GESEEITERESTRKEEEHPLPLMALNHVSRVCRSVKD 60

Query: 129 SVDFYTKVLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDNGVRPGGDKDHLDPLDN 188
           SVDFYTKVLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDNGVR  GDKDHLDPLDN
Sbjct: 61  SVDFYTKVLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDNGVRQ-GDKDHLDPLDN 120

Query: 189 HISFQCEDMEAMEEKLKELGVKYMRRTLEEEKGESIEQLFFNDPDGFMIEICNCENLKVV 248
           HISFQCEDMEAMEE+LKELGVKYMRRTL+EE GE IEQLFFNDPDGFMIEICNCENLKVV
Sbjct: 121 HISFQCEDMEAMEERLKELGVKYMRRTLDEENGELIEQLFFNDPDGFMIEICNCENLKVV 180

Query: 249 PAGSSGRIRLPAGRHNPPLES 269
           PAGSSGRIRLPAGRHNPPLES
Sbjct: 181 PAGSSGRIRLPAGRHNPPLES 198

[Close]

BLAST of Spg014170 vs. NCBI nr
Match: XP_022991929.1 (lactoylglutathione lyase [Cucurbita maxima])

HSP 1 Score: 350.5 bits (898), Expect = 1.3e-92
Identity = 180/201 (89.55%), Postives = 188/201 (93.53%), Query Frame = 0


Query: 69  MEEQIADRKA-IDEEVQTNNNELEESQEIRERDSTRKEEEHPLLLMALNHVSRVCRSVKD 128
           ME++IADRKA +  EVQ NN+   ES+EI ER+STRKEEEHPL LMALNHVSRVCRSVKD
Sbjct: 1   MEKEIADRKATVGNEVQENND--GESEEITERESTRKEEEHPLPLMALNHVSRVCRSVKD 60

Query: 129 SVDFYTKVLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDNGVRPGGDKDHLDPLDN 188
           SVDFYTKVLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDNGVR  GDKDHLDPLDN
Sbjct: 61  SVDFYTKVLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDNGVRL-GDKDHLDPLDN 120

Query: 189 HISFQCEDMEAMEEKLKELGVKYMRRTLEEEKGESIEQLFFNDPDGFMIEICNCENLKVV 248
           HISFQCEDMEAMEE+LKEL VKYMRRTL+EE GESIEQLFFNDPDGFMIEICNCENLKVV
Sbjct: 121 HISFQCEDMEAMEERLKELEVKYMRRTLDEENGESIEQLFFNDPDGFMIEICNCENLKVV 180

Query: 249 PAGSSGRIRLPAGRHNPPLES 269
           PAGSSGRIRLPAGRHNPPLES
Sbjct: 181 PAGSSGRIRLPAGRHNPPLES 198

[Close]

BLAST of Spg014170 vs. NCBI nr
Match: XP_038899611.1 (lactoylglutathione lyase [Benincasa hispida])

HSP 1 Score: 317.8 bits (813), Expect = 9.2e-83
Identity = 169/203 (83.25%), Postives = 181/203 (89.16%), Query Frame = 0


Query: 70  EEQIADRK-AIDEEVQTNNNELEESQEIRERDSTRK-EEEHPLLLMALNHVSRVCRSVKD 129
           ++QIADR   +D E+Q+ NN  EESQE     +TRK EEEHPL LMALNHVSRVCRSVKD
Sbjct: 3   QQQIADRNPTVDNEIQSKNN--EESQE-----TTRKQEEEHPLPLMALNHVSRVCRSVKD 62

Query: 130 SVDFYTKVLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDN-GVRPGG-DKDHLDPL 189
           SVDFYTKVLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSE+EDN GVR G  D DHLDPL
Sbjct: 63  SVDFYTKVLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEEEDNFGVRGGNKDDDHLDPL 122

Query: 190 DNHISFQCEDMEAMEEKLKELGVKYMRRTLEEEK-GESIEQLFFNDPDGFMIEICNCENL 249
           DNHISFQCEDMEA+EE+LK LGVKYMRRTLEEE+ GESIEQLFFNDPDGFMIEICNCEN+
Sbjct: 123 DNHISFQCEDMEAIEERLKVLGVKYMRRTLEEEENGESIEQLFFNDPDGFMIEICNCENI 182

Query: 250 KVVPAGSSGRIRLPAGRHNPPLE 268
           KVVP GSSGRI+LPAGRHNPPLE
Sbjct: 183 KVVPTGSSGRIKLPAGRHNPPLE 198

[Close]

BLAST of Spg014170 vs. ExPASy Swiss-Prot
Match: Q9CPU0 (Lactoylglutathione lyase OS=Mus musculus OX=10090 GN=Glo1 PE=1 SV=3)

HSP 1 Score: 49.3 bits (116), Expect = 8.0e-05
Identity = 37/146 (25.34%), Postives = 64/146 (43.84%), Query Frame = 0


Query: 126 KDSVDFYTKVLGFVLIER---------------------PQSFDFEGAWLFNYGVGIHLM 185
           K S+DFYT+VLG  L+++                     P+    + AW F+    + L 
Sbjct: 43  KKSLDFYTRVLGLTLLQKLDFPAMKFSLYFLAYEDKNDIPKDKSEKTAWTFSRKATLELT 102

Query: 186 Q---SEDEDNGVRPGGDKDHLDPLDNHISFQCEDMEAMEEKLKELGVKYMRRTLEEEKGE 245
               +ED++      G+ D       HI     D+ +  ++ +ELGVK++++    + G+
Sbjct: 103 HNWGTEDDETQSYHNGNSDPRG--FGHIGIAVPDVYSACKRFEELGVKFVKK---PDDGK 162

Query: 246 SIEQLFFNDPDGFMIEICNCENLKVV 248
                F  DPDG+ IEI N   +  +
Sbjct: 163 MKGLAFIQDPDGYWIEILNPNKIATI 183

[Close]

BLAST of Spg014170 vs. ExPASy Swiss-Prot
Match: Q6P7Q4 (Lactoylglutathione lyase OS=Rattus norvegicus OX=10116 GN=Glo1 PE=1 SV=3)

HSP 1 Score: 47.4 bits (111), Expect = 3.0e-04
Identity = 37/146 (25.34%), Postives = 62/146 (42.47%), Query Frame = 0


Query: 126 KDSVDFYTKVLGFVLIER---------------------PQSFDFEGAWLFNYGVGIHLM 185
           K S+DFYT+VLG  L+++                     P+      AW F+    + L 
Sbjct: 43  KKSLDFYTRVLGLTLLQKLDFPSMKFSLYFLAYEDKNDIPKDKTERTAWAFSRKATLELT 102

Query: 186 Q---SEDEDNGVRPGGDKDHLDPLDNHISFQCEDMEAMEEKLKELGVKYMRRTLEEEKGE 245
               +ED++      G+ D       HI     D+    ++ +ELGVK++++    + G+
Sbjct: 103 HNWGTEDDETQSYHNGNSDPRG--FGHIGIAVPDVYEACKRFEELGVKFVKK---PDDGK 162

Query: 246 SIEQLFFNDPDGFMIEICNCENLKVV 248
                F  DPDG+ IEI N   +  +
Sbjct: 163 MKGLAFVQDPDGYWIEILNPNKMATI 183

[Close]

BLAST of Spg014170 vs. ExPASy Swiss-Prot
Match: A4IS40 (Metallothiol transferase FosB OS=Geobacillus thermodenitrificans (strain NG80-2) OX=420246 GN=fosB PE=3 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 5.2e-04
Identity = 37/128 (28.91%), Postives = 59/128 (46.09%), Query Frame = 0


Query: 115 LNHVSRVCRSVKDSVDFYTKVLGFVLIERPQS---FDFEGAWLFNYGVGIHLMQSEDEDN 174
           +NH++     ++ S+ FY  V G  L+ + ++   FD  G WL      +++ Q      
Sbjct: 6   INHLTFSVSDLEKSIHFYQNVFGAKLLVKGRNLAYFDLNGIWL-----ALNVQQD----- 65

Query: 175 GVRPGGDKDHLDPLDNHISFQC--EDMEAMEEKLKELGVKYMRRTLEEEKGESIEQLFFN 234
              P  D  H      HI+F    ED + + EKLKELGV  +     +E+ +    ++F 
Sbjct: 66  --IPRNDIQH---SYTHIAFSVKEEDFDHVVEKLKELGVNILPGRERDERDK--RSVYFT 116

Query: 235 DPDGFMIE 238
           DPDG   E
Sbjct: 126 DPDGHKFE 116

[Close]

BLAST of Spg014170 vs. ExPASy TrEMBL
Match: A0A6J1GPD4 (lactoylglutathione lyase OS=Cucurbita moschata OX=3662 GN=LOC111455922 PE=4 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 3.6e-93
Identity = 180/201 (89.55%), Postives = 188/201 (93.53%), Query Frame = 0


Query: 69  MEEQIADRKA-IDEEVQTNNNELEESQEIRERDSTRKEEEHPLLLMALNHVSRVCRSVKD 128
           ME++IADRKA +  EVQ NN+   ES+EI ER+STRKEEEHPL LMALNHVSRVCRSVKD
Sbjct: 1   MEKEIADRKATVINEVQENND--GESEEITERESTRKEEEHPLPLMALNHVSRVCRSVKD 60

Query: 129 SVDFYTKVLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDNGVRPGGDKDHLDPLDN 188
           SVDFYTKVLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDNGVR  GDKDHLDPLDN
Sbjct: 61  SVDFYTKVLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDNGVRQ-GDKDHLDPLDN 120

Query: 189 HISFQCEDMEAMEEKLKELGVKYMRRTLEEEKGESIEQLFFNDPDGFMIEICNCENLKVV 248
           HISFQCEDMEAMEE+LKELGVKYMRRTL+EE GE IEQLFFNDPDGFMIEICNCENLKVV
Sbjct: 121 HISFQCEDMEAMEERLKELGVKYMRRTLDEENGELIEQLFFNDPDGFMIEICNCENLKVV 180

Query: 249 PAGSSGRIRLPAGRHNPPLES 269
           PAGSSGRIRLPAGRHNPPLES
Sbjct: 181 PAGSSGRIRLPAGRHNPPLES 198

[Close]

BLAST of Spg014170 vs. ExPASy TrEMBL
Match: A0A6J1JW68 (lactoylglutathione lyase OS=Cucurbita maxima OX=3661 GN=LOC111488425 PE=4 SV=1)

HSP 1 Score: 350.5 bits (898), Expect = 6.2e-93
Identity = 180/201 (89.55%), Postives = 188/201 (93.53%), Query Frame = 0


Query: 69  MEEQIADRKA-IDEEVQTNNNELEESQEIRERDSTRKEEEHPLLLMALNHVSRVCRSVKD 128
           ME++IADRKA +  EVQ NN+   ES+EI ER+STRKEEEHPL LMALNHVSRVCRSVKD
Sbjct: 1   MEKEIADRKATVGNEVQENND--GESEEITERESTRKEEEHPLPLMALNHVSRVCRSVKD 60

Query: 129 SVDFYTKVLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDNGVRPGGDKDHLDPLDN 188
           SVDFYTKVLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDNGVR  GDKDHLDPLDN
Sbjct: 61  SVDFYTKVLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDNGVRL-GDKDHLDPLDN 120

Query: 189 HISFQCEDMEAMEEKLKELGVKYMRRTLEEEKGESIEQLFFNDPDGFMIEICNCENLKVV 248
           HISFQCEDMEAMEE+LKEL VKYMRRTL+EE GESIEQLFFNDPDGFMIEICNCENLKVV
Sbjct: 121 HISFQCEDMEAMEERLKELEVKYMRRTLDEENGESIEQLFFNDPDGFMIEICNCENLKVV 180

Query: 249 PAGSSGRIRLPAGRHNPPLES 269
           PAGSSGRIRLPAGRHNPPLES
Sbjct: 181 PAGSSGRIRLPAGRHNPPLES 198

[Close]

BLAST of Spg014170 vs. ExPASy TrEMBL
Match: A0A0A0K9N2 (VOC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G420800 PE=4 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 3.4e-83
Identity = 177/269 (65.80%), Postives = 201/269 (74.72%), Query Frame = 0


Query: 5   QSISRTSSPHKLPRTAPSSSMAVGTLLPSPRVPYPPPLLPLLNQPPSTLFFHCSASIRRQ 64
           + I+ +SSPH+LPR  P                           PPS+    C A     
Sbjct: 30  REITFSSSPHQLPRRVP---------------------------PPSS----CMAVFIAV 89

Query: 65  SRTHMEEQ-IADRK--AIDEEVQTNNNELEESQEIRERDSTRKEEEHPLLLMALNHVSRV 124
           +  HME+Q IAD+   A++ E+Q NN E           +TRK+EEHPL LMALNHVSRV
Sbjct: 90  ALLHMEQQLIADQNPTAVNNEIQANNEE-----------TTRKQEEHPLPLMALNHVSRV 149

Query: 125 CRSVKDSVDFYTKVLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDN--GVRPGGDK 184
           C++VKDSV FYTKVLGFVLIERPQSFDFEGAWLFNYGVGIHLMQ+E++D+  GVR G DK
Sbjct: 150 CKNVKDSVHFYTKVLGFVLIERPQSFDFEGAWLFNYGVGIHLMQTEEDDDSVGVR-GSDK 209

Query: 185 DHLDPLDNHISFQCEDMEAMEEKLKELGVKYMRRTL-EEEKGESIEQLFFNDPDGFMIEI 244
           DHLDP+DNHISFQCEDMEAMEE+LKELGVKYMRRTL EEEKGE+IEQLFFNDPDGFMIEI
Sbjct: 210 DHLDPMDNHISFQCEDMEAMEERLKELGVKYMRRTLEEEEKGETIEQLFFNDPDGFMIEI 255

Query: 245 CNCENLKVVPAGSSGRIRLPAGRHNPPLE 268
           CNCENLK+VPAGSSG+IRLPAGRHNPPLE
Sbjct: 270 CNCENLKLVPAGSSGKIRLPAGRHNPPLE 255

[Close]

BLAST of Spg014170 vs. ExPASy TrEMBL
Match: A0A5A7UYM4 (Metallothiol transferase FosB OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00230 PE=4 SV=1)

HSP 1 Score: 314.3 bits (804), Expect = 4.9e-82
Identity = 166/205 (80.98%), Postives = 183/205 (89.27%), Query Frame = 0


Query: 69  MEEQ-IADRK--AIDEEVQTNNNELEESQEIRERDSTRKEEEHPLLLMALNHVSRVCRSV 128
           ME+Q IAD+   A++ E+Q NN   EESQE +     RK+EEHPL LMALNHVSRVC++V
Sbjct: 1   MEQQHIADQNPTAVNNEIQANN---EESQETK-----RKQEEHPLPLMALNHVSRVCKNV 60

Query: 129 KDSVDFYTKVLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDN--GVRPGGDKDHLD 188
           KDSV FYTKVLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSE++D+  GVR G DKDHLD
Sbjct: 61  KDSVHFYTKVLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEEDDDSVGVR-GSDKDHLD 120

Query: 189 PLDNHISFQCEDMEAMEEKLKELGVKYMRRTL-EEEKGESIEQLFFNDPDGFMIEICNCE 248
           P+DNHISFQCEDMEAMEE+LKELGVKYMRRTL EEEKGE+IEQLFFNDPDGFMIEICNCE
Sbjct: 121 PMDNHISFQCEDMEAMEERLKELGVKYMRRTLEEEEKGETIEQLFFNDPDGFMIEICNCE 180

Query: 249 NLKVVPAGSSGRIRLPAGRHNPPLE 268
           NLK+VPAGSSG+IRLPAGRHNPPLE
Sbjct: 181 NLKLVPAGSSGKIRLPAGRHNPPLE 196

[Close]

BLAST of Spg014170 vs. ExPASy TrEMBL
Match: A0A1S3CFR4 (metallothiol transferase FosB OS=Cucumis melo OX=3656 GN=LOC103499962 PE=4 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 1.9e-81
Identity = 165/205 (80.49%), Postives = 182/205 (88.78%), Query Frame = 0


Query: 69  MEEQ-IADRK--AIDEEVQTNNNELEESQEIRERDSTRKEEEHPLLLMALNHVSRVCRSV 128
           ME+Q IAD+   A++ E+Q NN   EESQE +     RK+EEHPL LMALNHVSRVC++V
Sbjct: 1   MEQQHIADQNPTAVNNEIQANN---EESQETK-----RKQEEHPLPLMALNHVSRVCKNV 60

Query: 129 KDSVDFYTKVLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDN--GVRPGGDKDHLD 188
           KDSV FYT VLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSE++D+  GVR G DKDHLD
Sbjct: 61  KDSVHFYTNVLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEEDDDSVGVR-GSDKDHLD 120

Query: 189 PLDNHISFQCEDMEAMEEKLKELGVKYMRRTL-EEEKGESIEQLFFNDPDGFMIEICNCE 248
           P+DNHISFQCEDMEAMEE+LKELGVKYMRRTL EEEKGE+IEQLFFNDPDGFMIEICNCE
Sbjct: 121 PMDNHISFQCEDMEAMEERLKELGVKYMRRTLEEEEKGETIEQLFFNDPDGFMIEICNCE 180

Query: 249 NLKVVPAGSSGRIRLPAGRHNPPLE 268
           NLK+VPAGSSG+IRLPAGRHNPPLE
Sbjct: 181 NLKLVPAGSSGKIRLPAGRHNPPLE 196

[Close]

BLAST of Spg014170 vs. TAIR 10
Match: AT2G28420.1 (Lactoylglutathione lyase / glyoxalase I family protein )

HSP 1 Score: 233.0 bits (593), Expect = 2.8e-61
Identity = 107/155 (69.03%), Postives = 133/155 (85.81%), Query Frame = 0


Query: 112 LMALNHVSRVCRSVKDSVDFYTKVLGFVLIERPQSFDFEGAWLFNYGVGIHLMQSEDEDN 171
           LMALNHVSR+C+ VK S++FYTKVLGFV IERP SFDF+GAWLFNYGVGIHL+Q++D+D 
Sbjct: 18  LMALNHVSRLCKDVKKSLEFYTKVLGFVEIERPASFDFDGAWLFNYGVGIHLVQAKDQD- 77

Query: 172 GVRPGGDKDHLDPLDNHISFQCEDMEAMEEKLKELGVKYMRRTLEEEKGESIEQLFFNDP 231
             +   D DHLDP+DNHISFQCEDMEA+E++LKE+ VKY++RT+ +EK  +I+QLFFNDP
Sbjct: 78  --KLPSDTDHLDPMDNHISFQCEDMEALEKRLKEVKVKYIKRTVGDEKDAAIDQLFFNDP 137

Query: 232 DGFMIEICNCENLKVVPAGSSGRIRLPAGRHNPPL 267
           DGFM+EICNCENL++VP  S+  IRLP  RH PP+
Sbjct: 138 DGFMVEICNCENLELVPCHSADAIRLPEDRHAPPV 169

[Close]

BLAST of Spg014170 vs. TAIR 10
Match: AT1G80160.1 (Lactoylglutathione lyase / glyoxalase I family protein )

HSP 1 Score: 173.7 bits (439), Expect = 2.0e-43
Identity = 82/153 (53.59%), Postives = 115/153 (75.16%), Query Frame = 0


Query: 103 RKEEEHPLLLMALNHVSRVCRSVKDSVDFYTKVLGFVLIERPQSFDFEGAWLFNYGVGIH 162
           + E  +PL + +LNH+S +CRSV++S+ FY  VLGF+ I RP SFDF+GAWLF +G+GIH
Sbjct: 2   KDETGNPLHIKSLNHISLLCRSVEESISFYQNVLGFLPIRRPDSFDFDGAWLFGHGIGIH 61

Query: 163 LMQSEDEDNGVRPGGDKDHLDPLDNHISFQCEDMEAMEEKLKELGVKYMRRTLEEEKGES 222
           L+QS + +  ++    K  ++P DNHISFQCE MEA+E+KLKE+ ++Y+R  + EE G  
Sbjct: 62  LLQSPEPEKLLK----KTEINPKDNHISFQCESMEAVEKKLKEMEIEYVRAVV-EEGGIQ 121

Query: 223 IEQLFFNDPDGFMIEICNCENLKVVP-AGSSGR 255
           ++QLFF+DPD FMIEICNC++L V+P AG   R
Sbjct: 122 VDQLFFHDPDAFMIEICNCDSLPVIPLAGEMAR 149

[Close]

BLAST of Spg014170 vs. TAIR 10
Match: AT1G15380.1 (Lactoylglutathione lyase / glyoxalase I family protein )

HSP 1 Score: 167.5 bits (423), Expect = 1.4e-41
Identity = 83/160 (51.88%), Postives = 117/160 (73.12%), Query Frame = 0


Query: 103 RKEEEHPLLLMALNHVSRVCRSVKDSVDFYTKVLGFVLIERPQSFDFEGAWLFNYGVGIH 162
           +++  +PL L +LNHVS +CRSV +S++FY KVLGF+ I RP+S +FEGAWLF +G+GIH
Sbjct: 2   KEDAGNPLHLTSLNHVSVLCRSVDESMNFYQKVLGFIPIRRPESLNFEGAWLFGHGIGIH 61

Query: 163 LMQSEDEDNGVRPGGDKDHLDPLDNHISFQCEDMEAMEEKLKELGVKYMRRTLEEEKGES 222
           L+ + + +   +    K  ++P DNHISFQCE M  +E+KL+E+G+ Y+ R L EE G  
Sbjct: 62  LLCAPEPEKLPK----KTAINPKDNHISFQCESMGVVEKKLEEMGIDYV-RALVEEGGIQ 121

Query: 223 IEQLFFNDPDGFMIEICNCENLKVVP-----AGSSGRIRL 258
           ++QLFF+DPDGFMIEICNC++L VVP     A S  R++L
Sbjct: 122 VDQLFFHDPDGFMIEICNCDSLPVVPLVGEMARSCSRVKL 156

[Close]

BLAST of Spg014170 vs. TAIR 10
Match: AT1G15380.2 (Lactoylglutathione lyase / glyoxalase I family protein )

HSP 1 Score: 167.5 bits (423), Expect = 1.4e-41
Identity = 83/160 (51.88%), Postives = 117/160 (73.12%), Query Frame = 0


Query: 103 RKEEEHPLLLMALNHVSRVCRSVKDSVDFYTKVLGFVLIERPQSFDFEGAWLFNYGVGIH 162
           +++  +PL L +LNHVS +CRSV +S++FY KVLGF+ I RP+S +FEGAWLF +G+GIH
Sbjct: 2   KEDAGNPLHLTSLNHVSVLCRSVDESMNFYQKVLGFIPIRRPESLNFEGAWLFGHGIGIH 61

Query: 163 LMQSEDEDNGVRPGGDKDHLDPLDNHISFQCEDMEAMEEKLKELGVKYMRRTLEEEKGES 222
           L+ + + +   +    K  ++P DNHISFQCE M  +E+KL+E+G+ Y+ R L EE G  
Sbjct: 62  LLCAPEPEKLPK----KTAINPKDNHISFQCESMGVVEKKLEEMGIDYV-RALVEEGGIQ 121

Query: 223 IEQLFFNDPDGFMIEICNCENLKVVP-----AGSSGRIRL 258
           ++QLFF+DPDGFMIEICNC++L VVP     A S  R++L
Sbjct: 122 VDQLFFHDPDGFMIEICNCDSLPVVPLVGEMARSCSRVKL 156

[Close]

BLAST of Spg014170 vs. TAIR 10
Match: AT1G80160.2 (Lactoylglutathione lyase / glyoxalase I family protein )

HSP 1 Score: 111.3 bits (277), Expect = 1.2e-24
Identity = 54/102 (52.94%), Postives = 76/102 (74.51%), Query Frame = 0


Query: 154 LFNYGVGIHLMQSEDEDNGVRPGGDKDHLDPLDNHISFQCEDMEAMEEKLKELGVKYMRR 213
           LF +G+GIHL+QS + +  ++    K  ++P DNHISFQCE MEA+E+KLKE+ ++Y+R 
Sbjct: 27  LFGHGIGIHLLQSPEPEKLLK----KTEINPKDNHISFQCESMEAVEKKLKEMEIEYVRA 86

Query: 214 TLEEEKGESIEQLFFNDPDGFMIEICNCENLKVVP-AGSSGR 255
            + EE G  ++QLFF+DPD FMIEICNC++L V+P AG   R
Sbjct: 87  VV-EEGGIQVDQLFFHDPDAFMIEICNCDSLPVIPLAGEMAR 123

The following BLAST results are available for this feature:

BLAST of Spg014170 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023548789.1	3.4e-93	89.55	lactoylglutathione lyase [Cucurbita pepo subsp. pepo]	[more]
KAG6575712.1	5.7e-93	89.05	Lactoylglutathione lyase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022953340.1	7.5e-93	89.55	lactoylglutathione lyase [Cucurbita moschata]	[more]
XP_022991929.1	1.3e-92	89.55	lactoylglutathione lyase [Cucurbita maxima]	[more]
XP_038899611.1	9.2e-83	83.25	lactoylglutathione lyase [Benincasa hispida]	[more]

BLAST of Spg014170 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9CPU0	8.0e-05	25.34	Lactoylglutathione lyase OS=Mus musculus OX=10090 GN=Glo1 PE=1 SV=3	[more]
Q6P7Q4	3.0e-04	25.34	Lactoylglutathione lyase OS=Rattus norvegicus OX=10116 GN=Glo1 PE=1 SV=3	[more]
A4IS40	5.2e-04	28.91	Metallothiol transferase FosB OS=Geobacillus thermodenitrificans (strain NG80-2)...	[more]

BLAST of Spg014170 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GPD4	3.6e-93	89.55	lactoylglutathione lyase OS=Cucurbita moschata OX=3662 GN=LOC111455922 PE=4 SV=1	[more]
A0A6J1JW68	6.2e-93	89.55	lactoylglutathione lyase OS=Cucurbita maxima OX=3661 GN=LOC111488425 PE=4 SV=1	[more]
A0A0A0K9N2	3.4e-83	65.80	VOC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G420800 PE=4 SV...	[more]
A0A5A7UYM4	4.9e-82	80.98	Metallothiol transferase FosB OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
A0A1S3CFR4	1.9e-81	80.49	metallothiol transferase FosB OS=Cucumis melo OX=3656 GN=LOC103499962 PE=4 SV=1	[more]

BLAST of Spg014170 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G28420.1	2.8e-61	69.03	Lactoylglutathione lyase / glyoxalase I family protein	[more]
AT1G80160.1	2.0e-43	53.59	Lactoylglutathione lyase / glyoxalase I family protein	[more]
AT1G15380.1	1.4e-41	51.88	Lactoylglutathione lyase / glyoxalase I family protein	[more]
AT1G15380.2	1.4e-41	51.88	Lactoylglutathione lyase / glyoxalase I family protein	[more]
AT1G80160.2	1.2e-24	52.94	Lactoylglutathione lyase / glyoxalase I family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 269..270
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 91..105
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 70..84
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..29
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 70..105
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..32
None	No IPR available	PANTHER	PTHR46142	FAMILY NOT NAMED	coord: 97..268
None	No IPR available	PANTHER	PTHR46142:SF8	EXPRESSED PROTEIN	coord: 97..268
None	No IPR available	CDD	cd07245	VOC_like	coord: 115..238 e-value: 6.69797E-43 score: 140.531
IPR029068	Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase	GENE3D	3.10.180.10		coord: 116..241 e-value: 1.3E-24 score: 88.8
IPR029068	Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase	SUPERFAMILY	54593	Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase	coord: 109..240
IPR004360	Glyoxalase/fosfomycin resistance/dioxygenase domain	PFAM	PF00903	Glyoxalase	coord: 115..238 e-value: 3.1E-11 score: 43.6
IPR037523	Vicinal oxygen chelate (VOC) domain	PROSITE	PS51819	VOC	coord: 114..241 score: 18.481756

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg014170.1	Spg014170.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016829	lyase activity
molecular_function	GO:0016740	transferase activity