Spg014128 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg014128 vs. NCBI nr
Match: XP_022152896.1 (stachyose synthase-like [Momordica charantia])

HSP 1 Score: 1594.3 bits (4127), Expect = 0.0e+00
Identity = 759/855 (88.77%), Postives = 807/855 (94.39%), Query Frame = 0

Query: 1   MSSLLKPKSLENHFDLSNGKINVKGVPLLSEVPSNVFFTPFSSIHQSSDAPLPLLQRVCG 60
           +SS  K ++LEN+ D S GKI+V+GVPLLSEVPSNVFF+PFSSI ++SDAPLPLLQRV G
Sbjct: 11  ISSFHKSETLENYIDCSKGKISVEGVPLLSEVPSNVFFSPFSSIFRTSDAPLPLLQRVHG 70

Query: 61  LSHKGGLLGFDQKQPSDRLMNSLGKFKGREFVSNFRFKTWWSTMWVGNSGSDLQMETQLV 120
           LS KGG LGF+Q QPSDRLMNSLGKF+GREFVS FRFKTWWSTMW+GNSGSDLQMETQLV
Sbjct: 71  LSRKGGFLGFEQMQPSDRLMNSLGKFEGREFVSVFRFKTWWSTMWIGNSGSDLQMETQLV 130

Query: 121 ILNVPEIRSYVVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYIHVSDN 180
           ILNVPEIRSYVVIIPIIEGSFRSALHPGTDGHV+IWAESGSTQVKASSF+AIAYIHVSDN
Sbjct: 131 ILNVPEIRSYVVIIPIIEGSFRSALHPGTDGHVLIWAESGSTQVKASSFNAIAYIHVSDN 190

Query: 181 PYNLMKEAYAAIRVHLNTFRLLEEKTVPTLVDRFGWCTWNAFFLNVNPVGVWNGVNDFAE 240
           PYNLMKEAYAAIRVHLNTFRLLEEK+VP L D+FGWCTWNAFFLNV PVGVWNGVNDFAE
Sbjct: 191 PYNLMKEAYAAIRVHLNTFRLLEEKSVPNLADKFGWCTWNAFFLNVEPVGVWNGVNDFAE 250

Query: 241 GGISLRFLIIDDGWQSINIDGEDPNQDMKYIVLGANQMTARLYKFEECDKFRKYKGGSLL 300
           GGISLRFLIIDDGWQSINIDGEDPN+DMK IVLGA+QMTARLYKFEECDKF+KYKGG+LL
Sbjct: 251 GGISLRFLIIDDGWQSINIDGEDPNRDMKNIVLGASQMTARLYKFEECDKFKKYKGGTLL 310

Query: 301 GPNAPPFDPKKPKLLISKAIEIVQAEKDRDNAIQSGVTDVSQFEAKVQQLKQELIDIFGE 360
           GPN PPFDPKKPKLLISK+IE+ +AEKDRDNAIQSGVTDVS+FEAKVQ+LKQE+ID+FG+
Sbjct: 311 GPNPPPFDPKKPKLLISKSIELDRAEKDRDNAIQSGVTDVSKFEAKVQKLKQEMIDMFGK 370

Query: 361 EEEEEESSVVSNGSGNCSYKADDFGMKAFTRDLRTKFKGLDDIYVWHALAGAWGGVRPDS 420
           EE  EESS VSN   + SY AD+FGMKAFTRDLRTKFKGLDDIYVWHALAGAWGGVRP S
Sbjct: 371 EEGGEESSAVSNDCESRSYTADNFGMKAFTRDLRTKFKGLDDIYVWHALAGAWGGVRPGS 430

Query: 421 THLSSKITPCKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQASDLFDSMHSYLSKVGITGV 480
           THLSSKI PCK SPGLDGTMTDLAVVK++EGKIGLVHPDQA DLFDSMHSYLSKVGITGV
Sbjct: 431 THLSSKIIPCKPSPGLDGTMTDLAVVKIMEGKIGLVHPDQAYDLFDSMHSYLSKVGITGV 490

Query: 481 KVDVIHTLEYVSEEYGGRVDLAKAYYKGLADSIVKNFKGTNLISSMQQCNDFFFLGTKQN 540
           KVDVIHTLEYVSEEYGGRVDLAKAYY GL +SI+KNFKGTNLISSMQQCNDFFFLGTKQN
Sbjct: 491 KVDVIHTLEYVSEEYGGRVDLAKAYYNGLTNSIIKNFKGTNLISSMQQCNDFFFLGTKQN 550

Query: 541 SIGRVSDDFWFHDPHGDPMGVYWLQGVHMVHCAFNSMWMGQIIQPDWDMFQSDHKCAKFH 600
           +IGRVSDDFWFHDP GDPMGVYWLQGVHMVHCA+NSMWMGQ IQPDWDMFQSDH+CAKFH
Sbjct: 551 AIGRVSDDFWFHDPQGDPMGVYWLQGVHMVHCAYNSMWMGQFIQPDWDMFQSDHQCAKFH 610

Query: 601 AGSRAICGGPVYVSDSVGGHDFDLIKKLAYPDGTIPRCQHFALPTRDCLFKNPLFDNKTV 660
           AGSRAICGGPVYVSDSVGGHDFDLIKKLAYPDGTIPRCQHFALPTRDCLFKNP+FDNKTV
Sbjct: 611 AGSRAICGGPVYVSDSVGGHDFDLIKKLAYPDGTIPRCQHFALPTRDCLFKNPIFDNKTV 670

Query: 661 LKIWNLNKYGGVIGAFNCQGAGWDRKEKRIKGRLECYKPMSTTVHVNDVEWDQKPEAAPM 720
           LKIWNLNKYGG+IGAFNCQGAGWD KE+RIKG  ECY PMSTTVHVNDVEW+QKPEAAPM
Sbjct: 671 LKIWNLNKYGGIIGAFNCQGAGWDPKEQRIKGHPECYNPMSTTVHVNDVEWNQKPEAAPM 730

Query: 721 GNFVEYLVYLNQAEQILLTTPKSEPLKVTLQPSTFEIFSFIPLRKLNSNIKFAPIGLTNM 780
           GNFVEY+VYLNQAEQIL TTPKSEPLKVTLQPSTFE+FSFIPLRK+ S+IKFAPIGLTNM
Sbjct: 731 GNFVEYIVYLNQAEQILHTTPKSEPLKVTLQPSTFELFSFIPLRKIGSSIKFAPIGLTNM 790

Query: 781 FNSSGTIQHLKYNDNGAELKVKGGGSFLAYSSGSPKKCLSNGIEVEFEWDSDGKLRLDLP 840
           FNSSGTIQHLKYN+NG ELKVKGGGSFLAYSSGSPKKC+SNG EVEFEWDS GKL  DLP
Sbjct: 791 FNSSGTIQHLKYNENGVELKVKGGGSFLAYSSGSPKKCVSNGTEVEFEWDSQGKLGFDLP 850

Query: 841 WIEEGGGISNLDIFF 856
             EE GG+SNLDIFF
Sbjct: 851 GNEEAGGVSNLDIFF 865

BLAST of Spg014128 vs. NCBI nr
Match: KAG7014305.1 (Stachyose synthase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1580.1 bits (4090), Expect = 0.0e+00
Identity = 756/857 (88.21%), Postives = 802/857 (93.58%), Query Frame = 0

Query: 1   MSSLLKPKSLENHFDLSNGKINVKGVPLLSEVPSNVFFTPFSSIHQSSDAPLPLLQRVCG 60
           MS L + KSL+N+  LSNG I+VKGVPLLS+V SNVFF+PFSS+ +SSDAPLPLLQRV  
Sbjct: 1   MSPLHQSKSLDNYVHLSNGNISVKGVPLLSDVSSNVFFSPFSSV-RSSDAPLPLLQRVHA 60

Query: 61  LSHKGGLLGFD--QKQPSDRLMNSLGKFKGREFVSNFRFKTWWSTMWVGNSGSDLQMETQ 120
           LSHKGG LGFD  Q QPSDRL+NSLGKF+GREFVS FRFKTWWSTMWVG+SGSD+QMETQ
Sbjct: 61  LSHKGGFLGFDQSQSQPSDRLINSLGKFEGREFVSIFRFKTWWSTMWVGSSGSDIQMETQ 120

Query: 121 LVILNVPEIRSYVVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYIHVS 180
            +ILNV EI SYVVIIPIIEGSFRSALHPG DGHVMIWAESGST VKASSFDAIAYIHVS
Sbjct: 121 WIILNVHEISSYVVIIPIIEGSFRSALHPGIDGHVMIWAESGSTHVKASSFDAIAYIHVS 180

Query: 181 DNPYNLMKEAYAAIRVHLNTFRLLEEKTVPTLVDRFGWCTWNAFFLNVNPVGVWNGVNDF 240
           DNPYNLMKEAYAAIRVHLNTFRLLEEK+VP LVDRFGWCTWNAFFLNVNPVGVWNGVNDF
Sbjct: 181 DNPYNLMKEAYAAIRVHLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVWNGVNDF 240

Query: 241 AEGGISLRFLIIDDGWQSINIDGEDPNQDMKYIVLGANQMTARLYKFEECDKFRKYKGGS 300
            EGGISLRFLIIDDGWQSIN+DGEDP++DMKYIVLG+NQMTARLYKFEECDKF KYKGG+
Sbjct: 241 TEGGISLRFLIIDDGWQSINVDGEDPHRDMKYIVLGSNQMTARLYKFEECDKFGKYKGGT 300

Query: 301 LLGPNAPPFDPKKPKLLISKAIEIVQAEKDRDNAIQSGVTDVSQFEAKVQQLKQELIDIF 360
           +LGPNAPPFDPKKPKLLISK+IEI  AEKDRDNAIQSGVTD+S+FEAKVQ+LKQELIDIF
Sbjct: 301 MLGPNAPPFDPKKPKLLISKSIEIDNAEKDRDNAIQSGVTDISKFEAKVQKLKQELIDIF 360

Query: 361 GEEEEEEESSVVSNGSGNCSYKADDFGMKAFTRDLRTKFKGLDDIYVWHALAGAWGGVRP 420
           G +++EEESSV SNG+GN SYK D+ GMKAFTRDLR+KFKGLDDIYVWHALAGAWGGVRP
Sbjct: 361 GNDQDEEESSVESNGNGNGSYKYDNIGMKAFTRDLRSKFKGLDDIYVWHALAGAWGGVRP 420

Query: 421 DSTHLSSKITPCKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQASDLFDSMHSYLSKVGIT 480
            STHL SKIT  KSSPGLDGTMTDLAVVKVIEGKI LVHPDQA DL+DSMHSYLS+ GIT
Sbjct: 421 GSTHLRSKITSLKSSPGLDGTMTDLAVVKVIEGKIALVHPDQAYDLYDSMHSYLSEAGIT 480

Query: 481 GVKVDVIHTLEYVSEEYGGRVDLAKAYYKGLADSIVKNFKGTNLISSMQQCNDFFFLGTK 540
           GVKVDVIHTLEYVSEEYGGRVDLAK YYKGL DSIVKNFKGTNLISSMQQCNDFFFLGTK
Sbjct: 481 GVKVDVIHTLEYVSEEYGGRVDLAKTYYKGLTDSIVKNFKGTNLISSMQQCNDFFFLGTK 540

Query: 541 QNSIGRVSDDFWFHDPHGDPMGVYWLQGVHMVHCAFNSMWMGQIIQPDWDMFQSDHKCAK 600
           QNSIGR SDDFWFHDPHGDPMG YWLQGVHMVHCA+NSMWMGQIIQPDWDMFQSDH CAK
Sbjct: 541 QNSIGRASDDFWFHDPHGDPMGEYWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDHVCAK 600

Query: 601 FHAGSRAICGGPVYVSDSVGGHDFDLIKKLAYPDGTIPRCQHFALPTRDCLFKNPLFDNK 660
           FHAGSRAICGGP+YVSDSVG HDFDL+K+L YPDGTIPRCQ+FALPTRDCLFKNPLFDNK
Sbjct: 601 FHAGSRAICGGPIYVSDSVGCHDFDLMKQLVYPDGTIPRCQYFALPTRDCLFKNPLFDNK 660

Query: 661 TVLKIWNLNKYGGVIGAFNCQGAGWDRKEKRIKGRLECYKPMSTTVHVNDVEWDQKPEAA 720
           T+LKIWNLNKYGGVIGAFNCQGAGWD KE+RIKGR ECYKPMSTTVHVNDVEWDQKPEAA
Sbjct: 661 TILKIWNLNKYGGVIGAFNCQGAGWDPKEQRIKGRPECYKPMSTTVHVNDVEWDQKPEAA 720

Query: 721 PMGNFVEYLVYLNQAEQILLTTPKSEPLKVTLQPSTFEIFSFIPLRKLNSNIKFAPIGLT 780
           PMGNFVEY+VYLNQAEQIL TTPKSEPL++TLQPSTFE+F+FIPL KL SNIKFAPIGLT
Sbjct: 721 PMGNFVEYVVYLNQAEQILHTTPKSEPLELTLQPSTFELFNFIPLEKLGSNIKFAPIGLT 780

Query: 781 NMFNSSGTIQHLKYNDNGAELKVKGGGSFLAYSSGSPKKCLSNGIEVEFEWDSDGKLRLD 840
           NMFNSSGTIQHLKYN NG ELKVKGGG+FLAYSSGSPKKCLSNGIEVEFEWD DGKL  D
Sbjct: 781 NMFNSSGTIQHLKYNKNGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWDYDGKLSFD 840

Query: 841 LPWIEEGGGISNLDIFF 856
           LPWIEE GG+SNLDIFF
Sbjct: 841 LPWIEEAGGVSNLDIFF 856

BLAST of Spg014128 vs. NCBI nr
Match: XP_022991745.1 (stachyose synthase-like [Cucurbita maxima])

HSP 1 Score: 1570.1 bits (4064), Expect = 0.0e+00
Identity = 747/846 (88.30%), Postives = 796/846 (94.09%), Query Frame = 0

Query: 12  NHFDLSNGKINVKGVPLLSEVPSNVFFTPFSSIHQSSDAPLPLLQRVCGLSHKGGLLGFD 71
           N+FDLSNG INVKGVPLLSEVPSNVFFTPFS + +SSDAP+PLLQRV  LSHKGG LGFD
Sbjct: 4   NYFDLSNGNINVKGVPLLSEVPSNVFFTPFSLV-RSSDAPVPLLQRVHALSHKGGFLGFD 63

Query: 72  --QKQPSDRLMNSLGKFKGREFVSNFRFKTWWSTMWVGNSGSDLQMETQLVILNVPEIRS 131
             Q QPSDRL+NSLGKFKGRE +S FRFKTWWSTMWVG+SGSD+QMETQ VILNVPEIRS
Sbjct: 64  QSQSQPSDRLINSLGKFKGRESLSIFRFKTWWSTMWVGSSGSDIQMETQWVILNVPEIRS 123

Query: 132 YVVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYIHVSDNPYNLMKEAY 191
           YVVIIPIIEGSFRSALHPGTDGHVMIWAESGST VKASSFDAIAYIHVSDNPYNLMKEAY
Sbjct: 124 YVVIIPIIEGSFRSALHPGTDGHVMIWAESGSTHVKASSFDAIAYIHVSDNPYNLMKEAY 183

Query: 192 AAIRVHLNTFRLLEEKTVPTLVDRFGWCTWNAFFLNVNPVGVWNGVNDFAEGGISLRFLI 251
           AAIRVHLNTFRLLEEK+VP LVDRFGWCTWNAFFLNVNPVGVW+GVNDF EGG+SLRFLI
Sbjct: 184 AAIRVHLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVWHGVNDFTEGGVSLRFLI 243

Query: 252 IDDGWQSINIDGEDPNQDMKYIVLGANQMTARLYKFEECDKFRKYKGGSLLGPNAPPFDP 311
           IDDGWQSIN+DGEDP++DMK+IVLGANQMTARLYKFEECDKF KYKGG++LGPNAPPFDP
Sbjct: 244 IDDGWQSINVDGEDPHRDMKHIVLGANQMTARLYKFEECDKFGKYKGGTMLGPNAPPFDP 303

Query: 312 KKPKLLISKAIEIVQAEKDRDNAIQSGVTDVSQFEAKVQQLKQELIDIFGEEEEEEESSV 371
           KKPKLLISK+IEI  AEKDRDNAIQSGVTD+S+FEAKVQ+LKQEL+DIFG +++EEE   
Sbjct: 304 KKPKLLISKSIEIDNAEKDRDNAIQSGVTDISKFEAKVQKLKQELMDIFGNDQDEEE--- 363

Query: 372 VSNGSGNCSYKADDFGMKAFTRDLRTKFKGLDDIYVWHALAGAWGGVRPDSTHLSSKITP 431
            +N + NC YK DD GMKAFTRDLRTKFKGLDDIYVWHALAGAWGGVRP STHL SKITP
Sbjct: 364 -NNRNENCCYKGDDMGMKAFTRDLRTKFKGLDDIYVWHALAGAWGGVRPGSTHLKSKITP 423

Query: 432 CKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQASDLFDSMHSYLSKVGITGVKVDVIHTLE 491
            KSSPGLDGTMTDLAVVKVIEGKI LVHPDQA DL+DSMHSYLSK GITGVKVDVIHTLE
Sbjct: 424 LKSSPGLDGTMTDLAVVKVIEGKIALVHPDQAYDLYDSMHSYLSKAGITGVKVDVIHTLE 483

Query: 492 YVSEEYGGRVDLAKAYYKGLADSIVKNFKGTNLISSMQQCNDFFFLGTKQNSIGRVSDDF 551
           YV+EEYGGRVDLAK YYKGL DSIVKNFKGTNLISSMQQCNDFFFLGTKQNSIGRVSDDF
Sbjct: 484 YVAEEYGGRVDLAKVYYKGLTDSIVKNFKGTNLISSMQQCNDFFFLGTKQNSIGRVSDDF 543

Query: 552 WFHDPHGDPMGVYWLQGVHMVHCAFNSMWMGQIIQPDWDMFQSDHKCAKFHAGSRAICGG 611
           WFHDPHGDP GVYWLQGVHMVHCA+NSMWMGQIIQPDWDMFQSDH CAKFHAGSRAICGG
Sbjct: 544 WFHDPHGDPTGVYWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDHMCAKFHAGSRAICGG 603

Query: 612 PVYVSDSVGGHDFDLIKKLAYPDGTIPRCQHFALPTRDCLFKNPLFDNKTVLKIWNLNKY 671
           PVYVSDSVGGHDFDLIKKLAYPDGTIPRCQHFALPTRDCLFKNPLFDNKT+LKIWNLNKY
Sbjct: 604 PVYVSDSVGGHDFDLIKKLAYPDGTIPRCQHFALPTRDCLFKNPLFDNKTILKIWNLNKY 663

Query: 672 GGVIGAFNCQGAGWDRKEKRIKGRLECYKPMSTTVHVNDVEWDQKPEAAPMGNFVEYLVY 731
           GG+IGAFNCQGAGW+RKEKRIKG  ECYKP+STTVHVNDVEWDQKPE APMGNFVEY+VY
Sbjct: 664 GGIIGAFNCQGAGWNRKEKRIKGYPECYKPISTTVHVNDVEWDQKPETAPMGNFVEYIVY 723

Query: 732 LNQAEQILLTTPKSEPLKVTLQPSTFEIFSFIPLRKLNSNIKFAPIGLTNMFNSSGTIQH 791
           LNQAE+IL TT KS+PL++TLQPSTFE+F+FIPL+KL+SNIKFAPIGLTNMFNSSGTIQH
Sbjct: 724 LNQAEKILHTTLKSKPLELTLQPSTFELFNFIPLKKLDSNIKFAPIGLTNMFNSSGTIQH 783

Query: 792 LKYNDNGAELKVKGGGSFLAYSSGSPKKCLSNGIEVEFEWDSDGKLRLDLPWIEEGGGIS 851
           LKYN+NG ELKVKG G+FLAYSSGSPKKC+SNG+EVEFEWDS GKL  DLPW+EE GG+S
Sbjct: 784 LKYNENGVELKVKGEGNFLAYSSGSPKKCISNGMEVEFEWDSKGKLSFDLPWMEEVGGVS 843

Query: 852 NLDIFF 856
           NLDI F
Sbjct: 844 NLDILF 844

BLAST of Spg014128 vs. NCBI nr
Match: XP_022953244.1 (stachyose synthase-like [Cucurbita moschata])

HSP 1 Score: 1567.0 bits (4056), Expect = 0.0e+00
Identity = 746/846 (88.18%), Postives = 796/846 (94.09%), Query Frame = 0

Query: 12  NHFDLSNGKINVKGVPLLSEVPSNVFFTPFSSIHQSSDAPLPLLQRVCGLSHKGGLLGFD 71
           N+FDLSNG INVKGVPLLSEVPSNVFF+PFSS+ +SSDAPLPLLQRV  LSHKGG LGFD
Sbjct: 4   NYFDLSNGNINVKGVPLLSEVPSNVFFSPFSSV-RSSDAPLPLLQRVHALSHKGGFLGFD 63

Query: 72  --QKQPSDRLMNSLGKFKGREFVSNFRFKTWWSTMWVGNSGSDLQMETQLVILNVPEIRS 131
             Q QPSDRL+NSLGKF+GRE +S FRFKTWWSTMWVG+SGSD+QMETQ VILNVPEIRS
Sbjct: 64  QSQSQPSDRLINSLGKFEGRESLSIFRFKTWWSTMWVGSSGSDIQMETQWVILNVPEIRS 123

Query: 132 YVVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYIHVSDNPYNLMKEAY 191
           YVVI+PIIEGSFRSALHPGTDGHVMIWAESGSTQVKAS FDAIAYIHVSDNPYNLMKEAY
Sbjct: 124 YVVIVPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASRFDAIAYIHVSDNPYNLMKEAY 183

Query: 192 AAIRVHLNTFRLLEEKTVPTLVDRFGWCTWNAFFLNVNPVGVWNGVNDFAEGGISLRFLI 251
           AAIR+HLNTFRLLEEK+VP LVDRFGWCTWNAFFLNVNPVGVW+GVNDF EGG+SLRFLI
Sbjct: 184 AAIRIHLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVWHGVNDFTEGGVSLRFLI 243

Query: 252 IDDGWQSINIDGEDPNQDMKYIVLGANQMTARLYKFEECDKFRKYKGGSLLGPNAPPFDP 311
           IDDGWQSIN DGEDP++DMK+IVLG+NQMTARLYKFEECDKF KYKGG++LGPNAPPFDP
Sbjct: 244 IDDGWQSINADGEDPHRDMKHIVLGSNQMTARLYKFEECDKFGKYKGGTMLGPNAPPFDP 303

Query: 312 KKPKLLISKAIEIVQAEKDRDNAIQSGVTDVSQFEAKVQQLKQELIDIFGEEEEEEESSV 371
           KKPKLLISK+IEI  AEKDRDNAIQSGVTD+S+FEAKVQ+LKQEL+DIFG +++EEE   
Sbjct: 304 KKPKLLISKSIEIDNAEKDRDNAIQSGVTDISKFEAKVQKLKQELMDIFGNDQDEEE--- 363

Query: 372 VSNGSGNCSYKADDFGMKAFTRDLRTKFKGLDDIYVWHALAGAWGGVRPDSTHLSSKITP 431
            +NG+GN  YK DD GMKAFTRDLRTKFKGLDDIYVWHALAGAWGGVRP STHL SKITP
Sbjct: 364 -NNGNGN--YKGDDMGMKAFTRDLRTKFKGLDDIYVWHALAGAWGGVRPGSTHLKSKITP 423

Query: 432 CKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQASDLFDSMHSYLSKVGITGVKVDVIHTLE 491
            KSSPGLDGTMTDLAVVKVIEGKI LVHPDQA DL+DSMHSYLSK GITGVKVDVIHTLE
Sbjct: 424 LKSSPGLDGTMTDLAVVKVIEGKIALVHPDQAYDLYDSMHSYLSKAGITGVKVDVIHTLE 483

Query: 492 YVSEEYGGRVDLAKAYYKGLADSIVKNFKGTNLISSMQQCNDFFFLGTKQNSIGRVSDDF 551
           YV+EEYGGRVDLAK YYKGL DSIVKNFKGTNLISSMQQCNDFFFLGTKQNSIGRVSDDF
Sbjct: 484 YVAEEYGGRVDLAKVYYKGLTDSIVKNFKGTNLISSMQQCNDFFFLGTKQNSIGRVSDDF 543

Query: 552 WFHDPHGDPMGVYWLQGVHMVHCAFNSMWMGQIIQPDWDMFQSDHKCAKFHAGSRAICGG 611
           WFHDP GDP GVYWLQGVHMVHCA+NSMWMGQIIQPDWDMFQSDH CAKFHAGSRAICGG
Sbjct: 544 WFHDPQGDPTGVYWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDHMCAKFHAGSRAICGG 603

Query: 612 PVYVSDSVGGHDFDLIKKLAYPDGTIPRCQHFALPTRDCLFKNPLFDNKTVLKIWNLNKY 671
           PVYVSDSVGGHDFDLIKKLAYPDGTIPRCQHFALPTRDCLFKNPLFDNKTVLKIWNLNKY
Sbjct: 604 PVYVSDSVGGHDFDLIKKLAYPDGTIPRCQHFALPTRDCLFKNPLFDNKTVLKIWNLNKY 663

Query: 672 GGVIGAFNCQGAGWDRKEKRIKGRLECYKPMSTTVHVNDVEWDQKPEAAPMGNFVEYLVY 731
           GG+IGAFNCQGAGWDRKEKRIKG  ECYKP+STTVHVNDVEWDQKPE APMGN VEY+VY
Sbjct: 664 GGIIGAFNCQGAGWDRKEKRIKGHPECYKPISTTVHVNDVEWDQKPETAPMGNSVEYIVY 723

Query: 732 LNQAEQILLTTPKSEPLKVTLQPSTFEIFSFIPLRKLNSNIKFAPIGLTNMFNSSGTIQH 791
           LNQAE+IL TTPKS+PL++TLQPSTFE+F+F P++KL+SNIKFAPIGLTNMFNSSGTIQH
Sbjct: 724 LNQAEEILHTTPKSKPLELTLQPSTFELFNFTPIKKLDSNIKFAPIGLTNMFNSSGTIQH 783

Query: 792 LKYNDNGAELKVKGGGSFLAYSSGSPKKCLSNGIEVEFEWDSDGKLRLDLPWIEEGGGIS 851
           LKYN NG ELKVKGGG+FLAYSSGSPKKC+SNG+EVEFEWDS GKL LDLPW+EE GG+S
Sbjct: 784 LKYNKNGVELKVKGGGNFLAYSSGSPKKCISNGMEVEFEWDSKGKLSLDLPWMEEVGGVS 842

Query: 852 NLDIFF 856
           NLDI F
Sbjct: 844 NLDILF 842

BLAST of Spg014128 vs. NCBI nr
Match: XP_023549394.1 (stachyose synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1566.2 bits (4054), Expect = 0.0e+00
Identity = 747/849 (87.99%), Postives = 792/849 (93.29%), Query Frame = 0

Query: 9   SLENHFDLSNGKINVKGVPLLSEVPSNVFFTPFSSIHQSSDAPLPLLQRVCGLSHKGGLL 68
           SL+N+  LSNG I+V+GVPLLS+VPSNVFF+PFSS+ +SSDAPLPL QRV  LSHKGG L
Sbjct: 3   SLDNYVHLSNGNISVQGVPLLSDVPSNVFFSPFSSV-RSSDAPLPLFQRVHALSHKGGFL 62

Query: 69  GFD--QKQPSDRLMNSLGKFKGREFVSNFRFKTWWSTMWVGNSGSDLQMETQLVILNVPE 128
           GFD  Q QPSDRL+NSLGKF+GREFVS FRFKTWWSTMWVG+SGSD+QMETQ VILNVPE
Sbjct: 63  GFDQTQSQPSDRLINSLGKFEGREFVSIFRFKTWWSTMWVGSSGSDIQMETQWVILNVPE 122

Query: 129 IRSYVVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYIHVSDNPYNLMK 188
           IRSYVVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYIHV DNPYNLMK
Sbjct: 123 IRSYVVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYIHVCDNPYNLMK 182

Query: 189 EAYAAIRVHLNTFRLLEEKTVPTLVDRFGWCTWNAFFLNVNPVGVWNGVNDFAEGGISLR 248
           EAYA IRVHLNTFRLLEEK+VP LVDRFGWCTWNAFFLNVNPVGVWNGVNDF EGG+SLR
Sbjct: 183 EAYATIRVHLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVWNGVNDFFEGGVSLR 242

Query: 249 FLIIDDGWQSINIDGEDPNQDMKYIVLGANQMTARLYKFEECDKFRKYKGGSLLGPNAPP 308
           FLIIDDGWQSINIDGEDP++DMKYIVLGANQMTARLYKFEECDKF KYKGG++LGPNAPP
Sbjct: 243 FLIIDDGWQSINIDGEDPHRDMKYIVLGANQMTARLYKFEECDKFEKYKGGTMLGPNAPP 302

Query: 309 FDPKKPKLLISKAIEIVQAEKDRDNAIQSGVTDVSQFEAKVQQLKQELIDIFGEEEEEEE 368
           FDPKKPKLLISK+IEI  AEK+RDNAIQSGV DV +FE +V++LKQELIDIF   + +EE
Sbjct: 303 FDPKKPKLLISKSIEIDNAEKNRDNAIQSGVNDVFKFETEVKKLKQELIDIFW-NDRDEE 362

Query: 369 SSVVSNGSGNCSYKADDFGMKAFTRDLRTKFKGLDDIYVWHALAGAWGGVRPDSTHLSSK 428
           ++V SNG+GNC YK DD GMKAFTRDLRTKFKGLDDIYVWHAL GAWGGVRP STHL SK
Sbjct: 363 NNVESNGNGNCCYKGDDMGMKAFTRDLRTKFKGLDDIYVWHALTGAWGGVRPGSTHLKSK 422

Query: 429 ITPCKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQASDLFDSMHSYLSKVGITGVKVDVIH 488
           ITP KSSPGLDGTMTDLAVVKVIEGKIGLVHPDQA DLFDSMHSYLS+ GITGVKVDVIH
Sbjct: 423 ITPLKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQAYDLFDSMHSYLSEAGITGVKVDVIH 482

Query: 489 TLEYVSEEYGGRVDLAKAYYKGLADSIVKNFKGTNLISSMQQCNDFFFLGTKQNSIGRVS 548
           TLEYVSEEYGGRVDLAK YYKGL DSIVKNFKGTNL+SSMQQCNDFFFLGTKQNSIGR S
Sbjct: 483 TLEYVSEEYGGRVDLAKTYYKGLTDSIVKNFKGTNLLSSMQQCNDFFFLGTKQNSIGRAS 542

Query: 549 DDFWFHDPHGDPMGVYWLQGVHMVHCAFNSMWMGQIIQPDWDMFQSDHKCAKFHAGSRAI 608
           DDFWFHDPHGDPMG+YWLQGVHMVHCA+NSMWMGQIIQPDWDMFQSDH CAKFHAGSRAI
Sbjct: 543 DDFWFHDPHGDPMGMYWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDHVCAKFHAGSRAI 602

Query: 609 CGGPVYVSDSVGGHDFDLIKKLAYPDGTIPRCQHFALPTRDCLFKNPLFDNKTVLKIWNL 668
           CGGP+YVSDSVG HDF LIKKLAYPDGTIPRC+HFALPTRDCLFKNPLFDNKTVLKIWNL
Sbjct: 603 CGGPIYVSDSVGSHDFYLIKKLAYPDGTIPRCRHFALPTRDCLFKNPLFDNKTVLKIWNL 662

Query: 669 NKYGGVIGAFNCQGAGWDRKEKRIKGRLECYKPMSTTVHVNDVEWDQKPEAAPMGNFVEY 728
           N YGGVIGAFNCQGAGWDRKE+RIKG  ECYKPMSTTVHVNDVEWDQKPEAAPMGNFVEY
Sbjct: 663 NMYGGVIGAFNCQGAGWDRKERRIKGHPECYKPMSTTVHVNDVEWDQKPEAAPMGNFVEY 722

Query: 729 LVYLNQAEQILLTTPKSEPLKVTLQPSTFEIFSFIPLRKLNSNIKFAPIGLTNMFNSSGT 788
           +VYLNQAEQIL TTPKSEPL++ L+PSTFE+F+FIPL+KL+SNIKFAPIGLTNMFNSSGT
Sbjct: 723 VVYLNQAEQILHTTPKSEPLELNLEPSTFELFNFIPLKKLDSNIKFAPIGLTNMFNSSGT 782

Query: 789 IQHLKYNDNGAELKVKGGGSFLAYSSGSPKKCLSNGIEVEFEWDSDGKLRLDLPWIEEGG 848
           IQHLKYN NG ELKVKGGG+FLAYSSGSPKKCLSNGIEVEFEWD DGKL  DL W EE G
Sbjct: 783 IQHLKYNKNGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWDYDGKLSFDLCWTEEAG 842

Query: 849 GISNLDIFF 856
           G SNLDIFF
Sbjct: 843 GFSNLDIFF 849

BLAST of Spg014128 vs. ExPASy Swiss-Prot
Match: Q93XK2 (Stachyose synthase OS=Pisum sativum OX=3888 GN=STS1 PE=1 SV=1)

HSP 1 Score: 1206.0 bits (3119), Expect = 0.0e+00
Identity = 560/856 (65.42%), Postives = 691/856 (80.72%), Query Frame = 0

Query: 2   SSLLKPKSLENHFDLSNGKINVKGVPLLSEVPSNVFFTPFSSI--HQSSDAPLPLLQRVC 61
           S+L+K +S+   FDLS  K  VKG PL  +VP NV F  FSSI     S+AP  LLQ+V 
Sbjct: 10  SNLIKTESI---FDLSERKFKVKGFPLFHDVPENVSFRSFSSICKPSESNAPPSLLQKVL 69

Query: 62  GLSHKGGLLGFDQKQPSDRLMNSLGKFKGREFVSNFRFKTWWSTMWVGNSGSDLQMETQL 121
             SHKGG  GF  + PSDRLMNS+G F G++F+S FRFKTWWST W+G SGSDLQMETQ 
Sbjct: 70  AYSHKGGFFGFSHETPSDRLMNSIGSFNGKDFLSIFRFKTWWSTQWIGKSGSDLQMETQW 129

Query: 122 VILNVPEIRSYVVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYIHVSD 181
           +++ VPE +SYVVIIPIIE  FRSAL PG + HV I AESGST+VK S+F++IAY+H S+
Sbjct: 130 ILIEVPETKSYVVIIPIIEKCFRSALFPGFNDHVKIIAESGSTKVKESTFNSIAYVHFSE 189

Query: 182 NPYNLMKEAYAAIRVHLNTFRLLEEKTVPTLVDRFGWCTWNAFFLNVNPVGVWNGVNDFA 241
           NPY+LMKEAY+AIRVHLN+FRLLEEKT+P LVD+FGWCTW+AF+L VNP+G+++G++DF+
Sbjct: 190 NPYDLMKEAYSAIRVHLNSFRLLEEKTIPNLVDKFGWCTWDAFYLTVNPIGIFHGLDDFS 249

Query: 242 EGGISLRFLIIDDGWQSINIDGEDPNQDMKYIVLGANQMTARLYKFEECDKFRKYKGGSL 301
           +GG+  RF+IIDDGWQSI+ DG DPN+D K +VLG  QM+ RL++F+EC KFRKY+ G L
Sbjct: 250 KGGVEPRFVIIDDGWQSISFDGYDPNEDAKNLVLGGEQMSGRLHRFDECYKFRKYESGLL 309

Query: 302 LGPNAPPFDPKKPKLLISKAIEIVQAEKDRDNAIQSGVTDVSQFEAKVQQLKQELIDIFG 361
           LGPN+PP+DP     LI K IE  +  K R+ AI S  +D+++ E+K++++ +E+ D+FG
Sbjct: 310 LGPNSPPYDPNNFTDLILKGIEHEKLRKKREEAISSKSSDLAEIESKIKKVVKEIDDLFG 369

Query: 362 EEEEEEESSVVSNGSGNCSYKADDFGMKAFTRDLRTKFKGLDDIYVWHALAGAWGGVRPD 421
            E+           SG  S    ++G+KAFT+DLRTKFKGLDD+YVWHAL GAWGGVRP+
Sbjct: 370 GEQ---------FSSGEKSEMKSEYGLKAFTKDLRTKFKGLDDVYVWHALCGAWGGVRPE 429

Query: 422 STHLSSKITPCKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQASDLFDSMHSYLSKVGITG 481
           +THL +KI PCK SPGLDGTM DLAVV++ +  +GLVHP QA++L+DSMHSYL++ GITG
Sbjct: 430 TTHLDTKIVPCKLSPGLDGTMEDLAVVEISKASLGLVHPSQANELYDSMHSYLAESGITG 489

Query: 482 VKVDVIHTLEYVSEEYGGRVDLAKAYYKGLADSIVKNFKGTNLISSMQQCNDFFFLGTKQ 541
           VKVDVIH+LEYV +EYGGRVDLAK YY+GL  SIVKNF G  +I+SMQ CNDFFFLGTKQ
Sbjct: 490 VKVDVIHSLEYVCDEYGGRVDLAKVYYEGLTKSIVKNFNGNGMIASMQHCNDFFFLGTKQ 549

Query: 542 NSIGRVSDDFWFHDPHGDPMGVYWLQGVHMVHCAFNSMWMGQIIQPDWDMFQSDHKCAKF 601
            S+GRV DDFWF DP+GDPMG +WLQGVHM+HC++NS+WMGQ+IQPDWDMFQSDH CAKF
Sbjct: 550 ISMGRVGDDFWFQDPNGDPMGSFWLQGVHMIHCSYNSLWMGQMIQPDWDMFQSDHVCAKF 609

Query: 602 HAGSRAICGGPVYVSDSVGGHDFDLIKKLAYPDGTIPRCQHFALPTRDCLFKNPLFDNKT 661
           HAGSRAICGGP+YVSD+VG HDFDLIKKL +PDGTIP+C +F LPTRDCLFKNPLFD+ T
Sbjct: 610 HAGSRAICGGPIYVSDNVGSHDFDLIKKLVFPDGTIPKCIYFPLPTRDCLFKNPLFDHTT 669

Query: 662 VLKIWNLNKYGGVIGAFNCQGAGWDRKEKRIKGRLECYKPMSTTVHVNDVEWDQKPEAAP 721
           VLKIWN NKYGGVIGAFNCQGAGWD   ++ +G  ECYKP+  TVHV +VEWDQK E + 
Sbjct: 670 VLKIWNFNKYGGVIGAFNCQGAGWDPIMQKFRGFPECYKPIPGTVHVTEVEWDQKEETSH 729

Query: 722 MGNFVEYLVYLNQAEQILLTTPKSEPLKVTLQPSTFEIFSFIPLRKLNSNIKFAPIGLTN 781
           +G   EY+VYLNQAE++ L T KSEP++ T+QPSTFE++SF+P+ KL   IKFAPIGLTN
Sbjct: 730 LGKAEEYVVYLNQAEELSLMTLKSEPIQFTIQPSTFELYSFVPVTKLCGGIKFAPIGLTN 789

Query: 782 MFNSSGTIQHLKYNDNGAELKVKGGGSFLAYSSGSPKKCLSNGIEVEFEWDSDGKLRLDL 841
           MFNS GT+  L+Y  NGA++KVKGGGSFLAYSS SPKK   NG EV+FEW  DGKL +++
Sbjct: 790 MFNSGGTVIDLEYVGNGAKIKVKGGGSFLAYSSESPKKFQLNGCEVDFEWLGDGKLCVNV 849

Query: 842 PWIEEGGGISNLDIFF 856
           PWIEE  G+S+++IFF
Sbjct: 850 PWIEEACGVSDMEIFF 853

BLAST of Spg014128 vs. ExPASy Swiss-Prot
Match: Q9SYJ4 (Probable galactinol--sucrose galactosyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=RFS4 PE=2 SV=3)

HSP 1 Score: 1061.6 bits (2744), Expect = 4.7e-309
Identity = 513/864 (59.38%), Postives = 653/864 (75.58%), Query Frame = 0

Query: 4   LLKPKSLENHFDLSNGKINVK-GVPLLSEVPSNVFFTPFSSIHQSSDAPLPLLQRVCGLS 63
           + KP    N F+LS G +  K   P+L +VP NV FTPFSS   S+DAPLP+L RV   +
Sbjct: 24  ITKPILQPNSFNLSEGSLCAKDSTPILFDVPQNVTFTPFSSHSISTDAPLPILLRVQANA 83

Query: 64  HKGGLLGFDQKQPSDRLMNSLGKFKGREFVSNFRFKTWWSTMWVGNSGSDLQMETQLVIL 123
           HKGG LGF ++ PSDRL NSLG+F+ REF+S FRFK WWST W+G SGSDLQ ETQ V+L
Sbjct: 84  HKGGFLGFTKESPSDRLTNSLGRFEDREFLSLFRFKMWWSTAWIGKSGSDLQAETQWVML 143

Query: 124 NVPEIRSYVVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYIHVSDNPY 183
            +PEI SYV IIP IEG+FR++L PG  G+V+I AESGST+VK SSF +IAYIH+ DNPY
Sbjct: 144 KIPEIDSYVAIIPTIEGAFRASLTPGEKGNVLICAESGSTKVKESSFKSIAYIHICDNPY 203

Query: 184 NLMKEAYAAIRVHLNTFRLLEEKTVPTLVDRFGWCTWNAFFLNVNPVGVWNGVNDFAEGG 243
           NLMKEA++A+RVH+NTF+LLEEK +P +VD+FGWCTW+A +L V+P  +W GV +F +GG
Sbjct: 204 NLMKEAFSALRVHMNTFKLLEEKKLPKIVDKFGWCTWDACYLTVDPATIWTGVKEFEDGG 263

Query: 244 ISLRFLIIDDGWQSINIDGEDPNQDMKYIVLGANQMTARLYKFEECDKFRKYKGGSLLGP 303
           +  +F+IIDDGWQSIN DG++ ++D + +VLG  QMTARL  F+EC KFR YKGGS +  
Sbjct: 264 VCPKFVIIDDGWQSINFDGDELDKDAENLVLGGEQMTARLTSFKECKKFRNYKGGSFITS 323

Query: 304 NAPPFDPKKPKLLISKAIEIVQAEKDRDNAI-QSGVTDVSQFEAKVQQLKQELIDIFGEE 363
           +A  F+P KPK+LI KA E +QA   R   + +SG  D+++ + K++ L +EL  +F E 
Sbjct: 324 DASHFNPLKPKMLIYKATERIQAIILRRKLVKESGEQDLTELDEKIKILSEELNAMFDEV 383

Query: 364 EEEEESSVVSNGSGNCSYKADDFGMKAFTRDLRTKFKGLDDIYVWHALAGAWGGVRPDS- 423
           E+EE     S GS + S      GM AFT+DLR +FK LDDIYVWHAL GAW GVRP++ 
Sbjct: 384 EKEE-----SLGSDDVS----GSGMAAFTKDLRLRFKSLDDIYVWHALCGAWNGVRPETM 443

Query: 424 THLSSKITPCKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQASDLFDSMHSYLSKVGITGV 483
             L +K+ P + SP L  TM DLAV KV+E  IGLVHP +A + +DSMHSYL+ VG+TG 
Sbjct: 444 MDLKAKVAPFELSPSLGATMADLAVDKVVEAGIGLVHPSKAHEFYDSMHSYLASVGVTGA 503

Query: 484 KVDVIHTLEYVSEEYGGRVDLAKAYYKGLADSIVKNFKGTNLISSMQQCNDFFFLGTKQN 543
           K+DV  TLE ++EE+GGRV+LAKAYY GL +S++KNF GT++I+SMQQCN+FFFL TKQ 
Sbjct: 504 KIDVFQTLESLAEEHGGRVELAKAYYDGLTESMIKNFNGTDVIASMQQCNEFFFLATKQI 563

Query: 544 SIGRVSDDFWFHDPHGDPMGVYWLQGVHMVHCAFNSMWMGQIIQPDWDMFQSDHKCAKFH 603
           SIGRV DDFW+ DP+GDP GVYWLQGVHM+HC++NS+WMGQ+IQPDWDMFQSDH CA++H
Sbjct: 564 SIGRVGDDFWWQDPYGDPQGVYWLQGVHMIHCSYNSIWMGQMIQPDWDMFQSDHVCAEYH 623

Query: 604 AGSRAICGGPVYVSDSVG--GHDFDLIKKLAYPDGTIPRCQHFALPTRDCLFKNPLFDNK 663
           A SRAICGGPVY+SD +G   H+FDLIKKLA+ DGTIPRC H+ALPTRD LFKNPLFD +
Sbjct: 624 AASRAICGGPVYLSDHLGKASHNFDLIKKLAFFDGTIPRCVHYALPTRDSLFKNPLFDKE 683

Query: 664 TVLKIWNLNKYGGVIGAFNCQGAGWDRKEKRIKGRLECYKPMSTTVHVNDVEWDQKPEAA 723
           ++LKI+N NK+GGVIG FNCQGAGW  +E R KG  ECY  +S TVHV+D+EWDQ PEAA
Sbjct: 684 SILKIFNFNKFGGVIGTFNCQGAGWSPEEHRFKGYKECYTTVSGTVHVSDIEWDQNPEAA 743

Query: 724 PMGNFV----EYLVYLNQAEQILLTTPKSEPLKVTLQPSTFEIFSFIPLRKL-NSNIKFA 783
             G+ V    +YLVY  Q+E+IL    KSE +K+TL+PS F++ SF+P+ +L +S ++FA
Sbjct: 744 --GSQVTYTGDYLVYKQQSEEILFMNSKSEAMKITLEPSAFDLLSFVPVTELVSSGVRFA 803

Query: 784 PIGLTNMFNSSGTIQHLKY-NDNGAELKVKGGGSFLAYSSGSPKKCLSNGIEVEFEWDSD 843
           P+GL NMFN  GT+Q +K   DN   + VKG G F+AYSS +P KC  N  E EF+W+ +
Sbjct: 804 PLGLINMFNCVGTVQDMKVTGDNSIRVDVKGEGRFMAYSSSAPVKCYLNDKEAEFKWEEE 863

Query: 844 -GKLRLDLPWIEEGGGISNLDIFF 856
            GKL   +PW+EE GGIS+L   F
Sbjct: 864 TGKLSFFVPWVEESGGISHLSFTF 876

BLAST of Spg014128 vs. ExPASy Swiss-Prot
Match: Q9FND9 (Probable galactinol--sucrose galactosyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=RFS5 PE=1 SV=1)

HSP 1 Score: 726.1 bits (1873), Expect = 4.6e-208
Identity = 373/854 (43.68%), Postives = 505/854 (59.13%), Query Frame = 0

Query: 14  FDLSNGKINVKGVPLLSEVPSNVFFTPFSSIHQSSDAPLPLLQRVCGLSHKGGLLGFD-Q 73
           F L +  +   G  +L++VP NV  T    +      PL +          G  +GF+  
Sbjct: 22  FRLEDSTLLANGQVVLTDVPVNVTLTSSPYLVDKDGVPLDV--------SAGSFIGFNLD 81

Query: 74  KQPSDRLMNSLGKFKGREFVSNFRFKTWWSTMWVGNSGSDLQMETQLVILNVP------- 133
            +P    + S+GK K   F+S FRFK WW+T WVG++G D++ ETQ++IL+         
Sbjct: 82  GEPKSHHVASIGKLKNIRFMSIFRFKVWWTTHWVGSNGRDIENETQIIILDQSGSDSGPG 141

Query: 134 --EIRSYVVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYIHVSDNPYN 193
               R YV+++P++EGSFRS+   G D  V +  ESGST+V  S F  I Y+H  D+P+ 
Sbjct: 142 SGSGRPYVLLLPLLEGSFRSSFQSGEDDDVAVCVESGSTEVTGSEFRQIVYVHAGDDPFK 201

Query: 194 LMKEAYAAIRVHLNTFRLLEEKTVPTLVDRFGWCTWNAFFLNVNPVGVWNGVNDFAEGGI 253
           L+K+A   IRVH+NTF+LLEEK+ P +VD+FGWCTW+AF+L VNP GV  GV    +GG 
Sbjct: 202 LVKDAMKVIRVHMNTFKLLEEKSPPGIVDKFGWCTWDAFYLTVNPDGVHKGVKCLVDGGC 261

Query: 254 SLRFLIIDDGWQSINIDGEDPNQDMKYIVLGANQMTARLYKFEECDKFRKYKGGSLLGPN 313
               ++IDDGWQSI  D +  + +   I +   QM  RL KFEE  KF+ Y         
Sbjct: 262 PPGLVLIDDGWQSIGHDSDGIDVEGMNITVAGEQMPCRLLKFEENHKFKDY--------- 321

Query: 314 APPFDPKKPKLLISKAIEIVQAEKDRDNAIQSGVTDVSQFEAKVQQLKQELIDIFGEEEE 373
                                + KD+                                  
Sbjct: 322 --------------------VSPKDQ---------------------------------- 381

Query: 374 EEESSVVSNGSGNCSYKADDFGMKAFTRDLRTKFKGLDDIYVWHALAGAWGGVRPDSTHL 433
                             +D GMKAF RDL+ +F  +D IYVWHAL G WGG+RP++  L
Sbjct: 382 ------------------NDVGMKAFVRDLKDEFSTVDYIYVWHALCGYWGGLRPEAPAL 441

Query: 434 -SSKITPCKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQASDLFDSMHSYLSKVGITGVKV 493
             S I   + SPGL  TM DLAV K+IE  IG   PD A + ++ +HS+L   GI GVKV
Sbjct: 442 PPSTIIRPELSPGLKLTMEDLAVDKIIETGIGFASPDLAKEFYEGLHSHLQNAGIDGVKV 501

Query: 494 DVIHTLEYVSEEYGGRVDLAKAYYKGLADSIVKNFKGTNLISSMQQCNDFFFLGTKQNSI 553
           DVIH LE + ++YGGRVDLAKAY+K L  S+ K+F G  +I+SM+ CNDF FLGT+  S+
Sbjct: 502 DVIHILEMLCQKYGGRVDLAKAYFKALTSSVNKHFNGNGVIASMEHCNDFMFLGTEAISL 561

Query: 554 GRVSDDFWFHDPHGDPMGVYWLQGVHMVHCAFNSMWMGQIIQPDWDMFQSDHKCAKFHAG 613
           GRV DDFW  DP GDP G +WLQG HMVHCA+NS+WMG  IQPDWDMFQS H CA+FHA 
Sbjct: 562 GRVGDDFWCTDPSGDPNGTFWLQGCHMVHCAYNSLWMGNFIQPDWDMFQSTHPCAEFHAA 621

Query: 614 SRAICGGPVYVSDSVGGHDFDLIKKLAYPDGTIPRCQHFALPTRDCLFKNPLFDNKTVLK 673
           SRAI GGP+Y+SD VG HDFDL+K+L  P+G+I RC+++ALPTRD LF++PL D KT+LK
Sbjct: 622 SRAISGGPIYISDCVGKHDFDLLKRLVLPNGSILRCEYYALPTRDRLFEDPLHDGKTMLK 681

Query: 674 IWNLNKYGGVIGAFNCQGAGWDRKEKRIKGRLECYKPMSTTVHVNDVEWDQKPEAAPMGN 733
           IWNLNKY GVIGAFNCQG GW R+ +R +   EC   ++ T    DVEW+       + N
Sbjct: 682 IWNLNKYTGVIGAFNCQGGGWCRETRRNQCFSECVNTLTATTSPKDVEWNSGSSPISIAN 741

Query: 734 FVEYLVYLNQAEQILLTTPKSEPLKVTLQPSTFEIFSFIPLRKLNSN-IKFAPIGLTNMF 793
             E+ ++L+Q++++LL +  ++ L++TL+P  FE+ +  P+  +  N ++FAPIGL NM 
Sbjct: 742 VEEFALFLSQSKKLLL-SGLNDDLELTLEPFKFELITVSPVVTIEGNSVRFAPIGLVNML 783

Query: 794 NSSGTIQHLKYNDNGAELKVKGGGSFLAYSSGSPKKCLSNGIEVEFEWDSDGKLRLDLPW 853
           N+SG I+ L YND   E+ V G G F  Y+S  P  CL +G  VEF ++ D  + + +PW
Sbjct: 802 NTSGAIRSLVYNDESVEVGVFGAGEFRVYASKKPVSCLIDGEVVEFGYE-DSMVMVQVPW 783

Query: 854 IEEGGGISNLDIFF 856
                G+S++   F
Sbjct: 862 -SGPDGLSSIQYLF 783

BLAST of Spg014128 vs. ExPASy Swiss-Prot
Match: Q5VQG4 (Galactinol--sucrose galactosyltransferase OS=Oryza sativa subsp. japonica OX=39947 GN=RFS PE=1 SV=1)

HSP 1 Score: 706.8 bits (1823), Expect = 2.9e-202
Identity = 367/873 (42.04%), Postives = 501/873 (57.39%), Query Frame = 0

Query: 1   MSSLLKPKSLENHFDLSNGKINVKGVPLLSEVPSNVFFTPFSSIHQSSDAPLPLLQRVCG 60
           +  L+KP      F L    + V G P L +VP+N+  TP S++  +SD P         
Sbjct: 19  VDGLIKPP----RFTLKGKDLAVDGHPFLLDVPANIRLTPASTLVPNSDVP--------- 78

Query: 61  LSHKGGLLGFDQKQPSDRLMNSLGKFKGREFVSNFRFKTWWSTMWVGNSGSDLQMETQLV 120
            +  G  LGFD     DR +  +GK +   F+S FRFK WW+T WVG +G D++ ETQ++
Sbjct: 79  AAAAGSFLGFDAPAAKDRHVVPIGKLRDTRFMSIFRFKVWWTTHWVGTNGRDVENETQMM 138

Query: 121 ILNVPEIRS-------YVVIIPIIEGSFRSALHPG-TDGHVMIWAESGSTQVKASSFDAI 180
           IL+    +S       YV+++PI+EG FR+ L  G  + +V +  ESGS+ V+ S F + 
Sbjct: 139 ILDQSGTKSSPTGPRPYVLLLPIVEGPFRACLESGKAEDYVHMVLESGSSTVRGSVFRSA 198

Query: 181 AYIHVSDNPYNLMKEAYAAIRVHLNTFRLLEEKTVPTLVDRFGWCTWNAFFLNVNPVGVW 240
            Y+H  D+P++L+K+A   +R HL TFRL+EEKT P +VD+FGWCTW+AF+L V+P GVW
Sbjct: 199 VYLHAGDDPFDLVKDAMRVVRAHLGTFRLMEEKTPPPIVDKFGWCTWDAFYLKVHPEGVW 258

Query: 241 NGVNDFAEGGISLRFLIIDDGWQSINIDGEDPNQDMKYI--VLGANQMTARLYKFEECDK 300
            GV   A+GG     ++IDDGWQSI  D +D     + +       QM  RL KF+E  K
Sbjct: 259 EGVRRLADGGCPPGLVLIDDGWQSICHDDDDLGSGAEGMNRTSAGEQMPCRLIKFQENYK 318

Query: 301 FRKYKGGSLLGPNAPPFDPKKPKLLISKAIEIVQAEKDRDNAIQSGVTDVSQFEAKVQQL 360
           FR+YKG                                                      
Sbjct: 319 FREYKG------------------------------------------------------ 378

Query: 361 KQELIDIFGEEEEEEESSVVSNGSGNCSYKADDFGMKAFTRDLRTKFKGLDDIYVWHALA 420
                                             GM  F R+++  F  ++ +YVWHAL 
Sbjct: 379 ----------------------------------GMGGFVREMKAAFPTVEQVYVWHALC 438

Query: 421 GAWGGVRPDSTHL-SSKITPCKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQASDLFDSMH 480
           G WGG+RP +  L  +K+   + SPGL  TM DLAV K++   +GLV P +A +L++ +H
Sbjct: 439 GYWGGLRPGAPGLPPAKVVAPRLSPGLQRTMEDLAVDKIVNNGVGLVDPRRARELYEGLH 498

Query: 481 SYLSKVGITGVKVDVIHTLEYVSEEYGGRVDLAKAYYKGLADSIVKNFKGTNLISSMQQC 540
           S+L   GI GVKVDVIH LE V EEYGGRV+LAKAY+ GL +S+ ++F G  +I+SM+ C
Sbjct: 499 SHLQASGIDGVKVDVIHLLEMVCEEYGGRVELAKAYFAGLTESVRRHFNGNGVIASMEHC 558

Query: 541 NDFFFLGTKQNSIGRVSDDFWFHDPHGDPMGVYWLQGVHMVHCAFNSMWMGQIIQPDWDM 600
           NDF  LGT+  ++GRV DDFW  DP GDP G +WLQG HMVHCA+NS+WMG  I PDWDM
Sbjct: 559 NDFMLLGTEAVALGRVGDDFWCTDPSGDPDGTFWLQGCHMVHCAYNSLWMGAFIHPDWDM 618

Query: 601 FQSDHKCAKFHAGSRAICGGPVYVSDSVGGHDFDLIKKLAYPDGTIPRCQHFALPTRDCL 660
           FQS H CA FHA SRA+ GGPVYVSD+VG HDFDL+++LA PDGTI RC+ +ALPTRDCL
Sbjct: 619 FQSTHPCAAFHAASRAVSGGPVYVSDAVGCHDFDLLRRLALPDGTILRCERYALPTRDCL 678

Query: 661 FKNPLFDNKTVLKIWNLNKYGGVIGAFNCQGAGWDRKEKRIKGRLECYKPMSTTVHVNDV 720
           F +PL D KT+LKIWN+NK+ GV+GAFNCQG GW R+ +R         P++      DV
Sbjct: 679 FADPLHDGKTMLKIWNVNKFSGVLGAFNCQGGGWSREARRNMCAAGFSVPVTARASPADV 738

Query: 721 EWDQKPEAAPMGNFVEYLVYLNQAEQILLTTPKSEPLKVTLQPSTFEIFSFIPLRKLNS- 780
           EW         G    + VY  +A ++ L   + E +++TL+P T+E+    P+R + S 
Sbjct: 739 EWSHGG-----GGGDRFAVYFVEARKLQLLR-RDESVELTLEPFTYELLVVAPVRAIVSP 783

Query: 781 --NIKFAPIGLTNMFNSSGTIQHL----KYNDNGAELKVKGGGSFLAYSSGSPKKCLSNG 840
              I FAPIGL NM N+ G +Q      K  D  AE+ VKG G  +AYSS  P+ C  NG
Sbjct: 799 ELGIGFAPIGLANMLNAGGAVQGFEAARKDGDVAAEVAVKGAGEMVAYSSARPRLCKVNG 783

Query: 841 IEVEFEWDSDGKLRLDLPWIEEGGGISNLDIFF 856
            + EF+++ DG + +D+PW      +S ++ F+
Sbjct: 859 QDAEFKYE-DGIVTVDVPWTGSSKKLSRVEYFY 783

BLAST of Spg014128 vs. ExPASy Swiss-Prot
Match: Q8VWN6 (Galactinol--sucrose galactosyltransferase OS=Pisum sativum OX=3888 GN=RFS PE=1 SV=1)

HSP 1 Score: 706.8 bits (1823), Expect = 2.9e-202
Identity = 364/833 (43.70%), Postives = 493/833 (59.18%), Query Frame = 0

Query: 23  VKGVPLLSEVPSNVFFT------PFSSIHQSSDAPLPLLQRVCGLSHKGGLLGFDQKQPS 82
           V G P L++VP N+  T      PF     + D    +      L  +G  +GF+  +  
Sbjct: 39  VNGHPFLTQVPPNITTTTTSTPSPFLDFKSNKDT---IANNNNTLQQQGCFVGFNTTEAK 98

Query: 83  DRLMNSLGKFKGREFVSNFRFKTWWSTMWVGNSGSDLQMETQLVIL--NVPEIRSYVVII 142
              +  LGK KG +F S FRFK WW+T WVG +G +LQ ETQ++IL  N+   R YV+++
Sbjct: 99  SHHVVPLGKLKGIKFTSIFRFKVWWTTHWVGTNGHELQHETQILILDKNISLGRPYVLLL 158

Query: 143 PIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYIHVSDNPYNLMKEAYAAIRV 202
           PI+E SFR++L PG + +V +  ESGST V  S+F A  Y+H+S++PY L+KEA   I+ 
Sbjct: 159 PILENSFRTSLQPGLNDYVDMSVESGSTHVTGSTFKACLYLHLSNDPYRLVKEAVKVIQT 218

Query: 203 HLNTFRLLEEKTVPTLVDRFGWCTWNAFFLNVNPVGVWNGVNDFAEGGISLRFLIIDDGW 262
            L TF+ LEEKT P+++++FGWCTW+AF+L V+P GVW GV    +GG    F+IIDDGW
Sbjct: 219 KLGTFKTLEEKTPPSIIEKFGWCTWDAFYLKVHPKGVWEGVKALTDGGCPPGFVIIDDGW 278

Query: 263 QSINIDGEDP--NQDMKYIVLGANQMTARLYKFEECDKFRKYKGGSLLGPNAPPFDPKKP 322
           QSI+ D +DP   +D         QM  RL K+EE  KFR+Y+ G               
Sbjct: 279 QSISHDDDDPVTERDGMNRTSAGEQMPCRLIKYEENYKFREYENG--------------- 338

Query: 323 KLLISKAIEIVQAEKDRDNAIQSGVTDVSQFEAKVQQLKQELIDIFGEEEEEEESSVVSN 382
                                                                      N
Sbjct: 339 ----------------------------------------------------------DN 398

Query: 383 GSGNCSYKADDFGMKAFTRDLRTKFKGLDDIYVWHALAGAWGGVRPDSTHL-SSKITPCK 442
           G           G+  F RDL+ +F+ ++ +YVWHAL G WGGVRP    +  +K+   K
Sbjct: 399 GGKK--------GLVGFVRDLKEEFRSVESVYVWHALCGYWGGVRPKVCGMPEAKVVVPK 458

Query: 443 SSPGLDGTMTDLAVVKVIEGKIGLVHPDQASDLFDSMHSYLSKVGITGVKVDVIHTLEYV 502
            SPG+  TM DLAV K++E  +GLV P+ A ++FD +HS+L   GI GVKVDVIH LE +
Sbjct: 459 LSPGVKMTMEDLAVDKIVENGVGLVPPNLAQEMFDGIHSHLESAGIDGVKVDVIHLLELL 518

Query: 503 SEEYGGRVDLAKAYYKGLADSIVKNFKGTNLISSMQQCNDFFFLGTKQNSIGRVSDDFWF 562
           SEEYGGRV+LAKAYYK L  S+ K+FKG  +I+SM+ CNDFF LGT+  S+GRV DDFW 
Sbjct: 519 SEEYGGRVELAKAYYKALTSSVNKHFKGNGVIASMEHCNDFFLLGTEAISLGRVGDDFWC 578

Query: 563 HDPHGDPMGVYWLQGVHMVHCAFNSMWMGQIIQPDWDMFQSDHKCAKFHAGSRAICGGPV 622
            DP GDP G YWLQG HMVHCA+NS+WMG  I PDWDMFQS H CA+FHA SRAI GGPV
Sbjct: 579 CDPSGDPNGTYWLQGCHMVHCAYNSLWMGNFIHPDWDMFQSTHPCAEFHAASRAISGGPV 638

Query: 623 YVSDSVGGHDFDLIKKLAYPDGTIPRCQHFALPTRDCLFKNPLFDNKTVLKIWNLNKYGG 682
           YVSD VG H+F L+K    PDG+I RCQH+ALPTRDCLF++PL + KT+LKIWNLNKY G
Sbjct: 639 YVSDCVGNHNFKLLKSFVLPDGSILRCQHYALPTRDCLFEDPLHNGKTMLKIWNLNKYAG 698

Query: 683 VIGAFNCQGAGWDRKEKRIKGRLECYKPMSTTVHVNDVEWDQKPEAAPMGNFVEYLVYLN 742
           V+G FNCQG GW  + +R K   E    ++      D+EW        +     + VY  
Sbjct: 699 VLGLFNCQGGGWCPETRRNKSASEFSHAVTCYASPEDIEWCNGKTPMDIKGVDVFAVYFF 758

Query: 743 QAEQILLTTPKSEPLKVTLQPSTFEIFSFIPLRKLNSN-IKFAPIGLTNMFNSSGTIQHL 802
           + +++ L    S+ L+V+L+P +FE+ +  PL+  +   I+FAPIGL NM NS G +Q L
Sbjct: 759 KEKKLSL-MKCSDRLEVSLEPFSFELMTVSPLKVFSKRLIQFAPIGLVNMLNSGGAVQSL 785

Query: 803 KYNDNGAELK--VKGGGSFLAYSSGSPKKCLSNGIEVEFEWDSDGKLRLDLPW 842
           +++D+ + +K  V+G G    ++S  P  C  +G+ VEF+++ D  +R+ + W
Sbjct: 819 EFDDSASLVKIGVRGCGELSVFASEKPVCCKIDGVSVEFDYE-DKMVRVQILW 785

BLAST of Spg014128 vs. ExPASy TrEMBL
Match: A0A6J1DJ41 (stachyose synthase-like OS=Momordica charantia OX=3673 GN=LOC111020515 PE=3 SV=1)

HSP 1 Score: 1594.3 bits (4127), Expect = 0.0e+00
Identity = 759/855 (88.77%), Postives = 807/855 (94.39%), Query Frame = 0

Query: 1   MSSLLKPKSLENHFDLSNGKINVKGVPLLSEVPSNVFFTPFSSIHQSSDAPLPLLQRVCG 60
           +SS  K ++LEN+ D S GKI+V+GVPLLSEVPSNVFF+PFSSI ++SDAPLPLLQRV G
Sbjct: 11  ISSFHKSETLENYIDCSKGKISVEGVPLLSEVPSNVFFSPFSSIFRTSDAPLPLLQRVHG 70

Query: 61  LSHKGGLLGFDQKQPSDRLMNSLGKFKGREFVSNFRFKTWWSTMWVGNSGSDLQMETQLV 120
           LS KGG LGF+Q QPSDRLMNSLGKF+GREFVS FRFKTWWSTMW+GNSGSDLQMETQLV
Sbjct: 71  LSRKGGFLGFEQMQPSDRLMNSLGKFEGREFVSVFRFKTWWSTMWIGNSGSDLQMETQLV 130

Query: 121 ILNVPEIRSYVVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYIHVSDN 180
           ILNVPEIRSYVVIIPIIEGSFRSALHPGTDGHV+IWAESGSTQVKASSF+AIAYIHVSDN
Sbjct: 131 ILNVPEIRSYVVIIPIIEGSFRSALHPGTDGHVLIWAESGSTQVKASSFNAIAYIHVSDN 190

Query: 181 PYNLMKEAYAAIRVHLNTFRLLEEKTVPTLVDRFGWCTWNAFFLNVNPVGVWNGVNDFAE 240
           PYNLMKEAYAAIRVHLNTFRLLEEK+VP L D+FGWCTWNAFFLNV PVGVWNGVNDFAE
Sbjct: 191 PYNLMKEAYAAIRVHLNTFRLLEEKSVPNLADKFGWCTWNAFFLNVEPVGVWNGVNDFAE 250

Query: 241 GGISLRFLIIDDGWQSINIDGEDPNQDMKYIVLGANQMTARLYKFEECDKFRKYKGGSLL 300
           GGISLRFLIIDDGWQSINIDGEDPN+DMK IVLGA+QMTARLYKFEECDKF+KYKGG+LL
Sbjct: 251 GGISLRFLIIDDGWQSINIDGEDPNRDMKNIVLGASQMTARLYKFEECDKFKKYKGGTLL 310

Query: 301 GPNAPPFDPKKPKLLISKAIEIVQAEKDRDNAIQSGVTDVSQFEAKVQQLKQELIDIFGE 360
           GPN PPFDPKKPKLLISK+IE+ +AEKDRDNAIQSGVTDVS+FEAKVQ+LKQE+ID+FG+
Sbjct: 311 GPNPPPFDPKKPKLLISKSIELDRAEKDRDNAIQSGVTDVSKFEAKVQKLKQEMIDMFGK 370

Query: 361 EEEEEESSVVSNGSGNCSYKADDFGMKAFTRDLRTKFKGLDDIYVWHALAGAWGGVRPDS 420
           EE  EESS VSN   + SY AD+FGMKAFTRDLRTKFKGLDDIYVWHALAGAWGGVRP S
Sbjct: 371 EEGGEESSAVSNDCESRSYTADNFGMKAFTRDLRTKFKGLDDIYVWHALAGAWGGVRPGS 430

Query: 421 THLSSKITPCKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQASDLFDSMHSYLSKVGITGV 480
           THLSSKI PCK SPGLDGTMTDLAVVK++EGKIGLVHPDQA DLFDSMHSYLSKVGITGV
Sbjct: 431 THLSSKIIPCKPSPGLDGTMTDLAVVKIMEGKIGLVHPDQAYDLFDSMHSYLSKVGITGV 490

Query: 481 KVDVIHTLEYVSEEYGGRVDLAKAYYKGLADSIVKNFKGTNLISSMQQCNDFFFLGTKQN 540
           KVDVIHTLEYVSEEYGGRVDLAKAYY GL +SI+KNFKGTNLISSMQQCNDFFFLGTKQN
Sbjct: 491 KVDVIHTLEYVSEEYGGRVDLAKAYYNGLTNSIIKNFKGTNLISSMQQCNDFFFLGTKQN 550

Query: 541 SIGRVSDDFWFHDPHGDPMGVYWLQGVHMVHCAFNSMWMGQIIQPDWDMFQSDHKCAKFH 600
           +IGRVSDDFWFHDP GDPMGVYWLQGVHMVHCA+NSMWMGQ IQPDWDMFQSDH+CAKFH
Sbjct: 551 AIGRVSDDFWFHDPQGDPMGVYWLQGVHMVHCAYNSMWMGQFIQPDWDMFQSDHQCAKFH 610

Query: 601 AGSRAICGGPVYVSDSVGGHDFDLIKKLAYPDGTIPRCQHFALPTRDCLFKNPLFDNKTV 660
           AGSRAICGGPVYVSDSVGGHDFDLIKKLAYPDGTIPRCQHFALPTRDCLFKNP+FDNKTV
Sbjct: 611 AGSRAICGGPVYVSDSVGGHDFDLIKKLAYPDGTIPRCQHFALPTRDCLFKNPIFDNKTV 670

Query: 661 LKIWNLNKYGGVIGAFNCQGAGWDRKEKRIKGRLECYKPMSTTVHVNDVEWDQKPEAAPM 720
           LKIWNLNKYGG+IGAFNCQGAGWD KE+RIKG  ECY PMSTTVHVNDVEW+QKPEAAPM
Sbjct: 671 LKIWNLNKYGGIIGAFNCQGAGWDPKEQRIKGHPECYNPMSTTVHVNDVEWNQKPEAAPM 730

Query: 721 GNFVEYLVYLNQAEQILLTTPKSEPLKVTLQPSTFEIFSFIPLRKLNSNIKFAPIGLTNM 780
           GNFVEY+VYLNQAEQIL TTPKSEPLKVTLQPSTFE+FSFIPLRK+ S+IKFAPIGLTNM
Sbjct: 731 GNFVEYIVYLNQAEQILHTTPKSEPLKVTLQPSTFELFSFIPLRKIGSSIKFAPIGLTNM 790

Query: 781 FNSSGTIQHLKYNDNGAELKVKGGGSFLAYSSGSPKKCLSNGIEVEFEWDSDGKLRLDLP 840
           FNSSGTIQHLKYN+NG ELKVKGGGSFLAYSSGSPKKC+SNG EVEFEWDS GKL  DLP
Sbjct: 791 FNSSGTIQHLKYNENGVELKVKGGGSFLAYSSGSPKKCVSNGTEVEFEWDSQGKLGFDLP 850

Query: 841 WIEEGGGISNLDIFF 856
             EE GG+SNLDIFF
Sbjct: 851 GNEEAGGVSNLDIFF 865

BLAST of Spg014128 vs. ExPASy TrEMBL
Match: A0A6J1JTU2 (stachyose synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111488278 PE=3 SV=1)

HSP 1 Score: 1570.1 bits (4064), Expect = 0.0e+00
Identity = 747/846 (88.30%), Postives = 796/846 (94.09%), Query Frame = 0

Query: 12  NHFDLSNGKINVKGVPLLSEVPSNVFFTPFSSIHQSSDAPLPLLQRVCGLSHKGGLLGFD 71
           N+FDLSNG INVKGVPLLSEVPSNVFFTPFS + +SSDAP+PLLQRV  LSHKGG LGFD
Sbjct: 4   NYFDLSNGNINVKGVPLLSEVPSNVFFTPFSLV-RSSDAPVPLLQRVHALSHKGGFLGFD 63

Query: 72  --QKQPSDRLMNSLGKFKGREFVSNFRFKTWWSTMWVGNSGSDLQMETQLVILNVPEIRS 131
             Q QPSDRL+NSLGKFKGRE +S FRFKTWWSTMWVG+SGSD+QMETQ VILNVPEIRS
Sbjct: 64  QSQSQPSDRLINSLGKFKGRESLSIFRFKTWWSTMWVGSSGSDIQMETQWVILNVPEIRS 123

Query: 132 YVVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYIHVSDNPYNLMKEAY 191
           YVVIIPIIEGSFRSALHPGTDGHVMIWAESGST VKASSFDAIAYIHVSDNPYNLMKEAY
Sbjct: 124 YVVIIPIIEGSFRSALHPGTDGHVMIWAESGSTHVKASSFDAIAYIHVSDNPYNLMKEAY 183

Query: 192 AAIRVHLNTFRLLEEKTVPTLVDRFGWCTWNAFFLNVNPVGVWNGVNDFAEGGISLRFLI 251
           AAIRVHLNTFRLLEEK+VP LVDRFGWCTWNAFFLNVNPVGVW+GVNDF EGG+SLRFLI
Sbjct: 184 AAIRVHLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVWHGVNDFTEGGVSLRFLI 243

Query: 252 IDDGWQSINIDGEDPNQDMKYIVLGANQMTARLYKFEECDKFRKYKGGSLLGPNAPPFDP 311
           IDDGWQSIN+DGEDP++DMK+IVLGANQMTARLYKFEECDKF KYKGG++LGPNAPPFDP
Sbjct: 244 IDDGWQSINVDGEDPHRDMKHIVLGANQMTARLYKFEECDKFGKYKGGTMLGPNAPPFDP 303

Query: 312 KKPKLLISKAIEIVQAEKDRDNAIQSGVTDVSQFEAKVQQLKQELIDIFGEEEEEEESSV 371
           KKPKLLISK+IEI  AEKDRDNAIQSGVTD+S+FEAKVQ+LKQEL+DIFG +++EEE   
Sbjct: 304 KKPKLLISKSIEIDNAEKDRDNAIQSGVTDISKFEAKVQKLKQELMDIFGNDQDEEE--- 363

Query: 372 VSNGSGNCSYKADDFGMKAFTRDLRTKFKGLDDIYVWHALAGAWGGVRPDSTHLSSKITP 431
            +N + NC YK DD GMKAFTRDLRTKFKGLDDIYVWHALAGAWGGVRP STHL SKITP
Sbjct: 364 -NNRNENCCYKGDDMGMKAFTRDLRTKFKGLDDIYVWHALAGAWGGVRPGSTHLKSKITP 423

Query: 432 CKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQASDLFDSMHSYLSKVGITGVKVDVIHTLE 491
            KSSPGLDGTMTDLAVVKVIEGKI LVHPDQA DL+DSMHSYLSK GITGVKVDVIHTLE
Sbjct: 424 LKSSPGLDGTMTDLAVVKVIEGKIALVHPDQAYDLYDSMHSYLSKAGITGVKVDVIHTLE 483

Query: 492 YVSEEYGGRVDLAKAYYKGLADSIVKNFKGTNLISSMQQCNDFFFLGTKQNSIGRVSDDF 551
           YV+EEYGGRVDLAK YYKGL DSIVKNFKGTNLISSMQQCNDFFFLGTKQNSIGRVSDDF
Sbjct: 484 YVAEEYGGRVDLAKVYYKGLTDSIVKNFKGTNLISSMQQCNDFFFLGTKQNSIGRVSDDF 543

Query: 552 WFHDPHGDPMGVYWLQGVHMVHCAFNSMWMGQIIQPDWDMFQSDHKCAKFHAGSRAICGG 611
           WFHDPHGDP GVYWLQGVHMVHCA+NSMWMGQIIQPDWDMFQSDH CAKFHAGSRAICGG
Sbjct: 544 WFHDPHGDPTGVYWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDHMCAKFHAGSRAICGG 603

Query: 612 PVYVSDSVGGHDFDLIKKLAYPDGTIPRCQHFALPTRDCLFKNPLFDNKTVLKIWNLNKY 671
           PVYVSDSVGGHDFDLIKKLAYPDGTIPRCQHFALPTRDCLFKNPLFDNKT+LKIWNLNKY
Sbjct: 604 PVYVSDSVGGHDFDLIKKLAYPDGTIPRCQHFALPTRDCLFKNPLFDNKTILKIWNLNKY 663

Query: 672 GGVIGAFNCQGAGWDRKEKRIKGRLECYKPMSTTVHVNDVEWDQKPEAAPMGNFVEYLVY 731
           GG+IGAFNCQGAGW+RKEKRIKG  ECYKP+STTVHVNDVEWDQKPE APMGNFVEY+VY
Sbjct: 664 GGIIGAFNCQGAGWNRKEKRIKGYPECYKPISTTVHVNDVEWDQKPETAPMGNFVEYIVY 723

Query: 732 LNQAEQILLTTPKSEPLKVTLQPSTFEIFSFIPLRKLNSNIKFAPIGLTNMFNSSGTIQH 791
           LNQAE+IL TT KS+PL++TLQPSTFE+F+FIPL+KL+SNIKFAPIGLTNMFNSSGTIQH
Sbjct: 724 LNQAEKILHTTLKSKPLELTLQPSTFELFNFIPLKKLDSNIKFAPIGLTNMFNSSGTIQH 783

Query: 792 LKYNDNGAELKVKGGGSFLAYSSGSPKKCLSNGIEVEFEWDSDGKLRLDLPWIEEGGGIS 851
           LKYN+NG ELKVKG G+FLAYSSGSPKKC+SNG+EVEFEWDS GKL  DLPW+EE GG+S
Sbjct: 784 LKYNENGVELKVKGEGNFLAYSSGSPKKCISNGMEVEFEWDSKGKLSFDLPWMEEVGGVS 843

Query: 852 NLDIFF 856
           NLDI F
Sbjct: 844 NLDILF 844

BLAST of Spg014128 vs. ExPASy TrEMBL
Match: A0A6J1GP33 (stachyose synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111455850 PE=3 SV=1)

HSP 1 Score: 1567.0 bits (4056), Expect = 0.0e+00
Identity = 746/846 (88.18%), Postives = 796/846 (94.09%), Query Frame = 0

Query: 12  NHFDLSNGKINVKGVPLLSEVPSNVFFTPFSSIHQSSDAPLPLLQRVCGLSHKGGLLGFD 71
           N+FDLSNG INVKGVPLLSEVPSNVFF+PFSS+ +SSDAPLPLLQRV  LSHKGG LGFD
Sbjct: 4   NYFDLSNGNINVKGVPLLSEVPSNVFFSPFSSV-RSSDAPLPLLQRVHALSHKGGFLGFD 63

Query: 72  --QKQPSDRLMNSLGKFKGREFVSNFRFKTWWSTMWVGNSGSDLQMETQLVILNVPEIRS 131
             Q QPSDRL+NSLGKF+GRE +S FRFKTWWSTMWVG+SGSD+QMETQ VILNVPEIRS
Sbjct: 64  QSQSQPSDRLINSLGKFEGRESLSIFRFKTWWSTMWVGSSGSDIQMETQWVILNVPEIRS 123

Query: 132 YVVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYIHVSDNPYNLMKEAY 191
           YVVI+PIIEGSFRSALHPGTDGHVMIWAESGSTQVKAS FDAIAYIHVSDNPYNLMKEAY
Sbjct: 124 YVVIVPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASRFDAIAYIHVSDNPYNLMKEAY 183

Query: 192 AAIRVHLNTFRLLEEKTVPTLVDRFGWCTWNAFFLNVNPVGVWNGVNDFAEGGISLRFLI 251
           AAIR+HLNTFRLLEEK+VP LVDRFGWCTWNAFFLNVNPVGVW+GVNDF EGG+SLRFLI
Sbjct: 184 AAIRIHLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVWHGVNDFTEGGVSLRFLI 243

Query: 252 IDDGWQSINIDGEDPNQDMKYIVLGANQMTARLYKFEECDKFRKYKGGSLLGPNAPPFDP 311
           IDDGWQSIN DGEDP++DMK+IVLG+NQMTARLYKFEECDKF KYKGG++LGPNAPPFDP
Sbjct: 244 IDDGWQSINADGEDPHRDMKHIVLGSNQMTARLYKFEECDKFGKYKGGTMLGPNAPPFDP 303

Query: 312 KKPKLLISKAIEIVQAEKDRDNAIQSGVTDVSQFEAKVQQLKQELIDIFGEEEEEEESSV 371
           KKPKLLISK+IEI  AEKDRDNAIQSGVTD+S+FEAKVQ+LKQEL+DIFG +++EEE   
Sbjct: 304 KKPKLLISKSIEIDNAEKDRDNAIQSGVTDISKFEAKVQKLKQELMDIFGNDQDEEE--- 363

Query: 372 VSNGSGNCSYKADDFGMKAFTRDLRTKFKGLDDIYVWHALAGAWGGVRPDSTHLSSKITP 431
            +NG+GN  YK DD GMKAFTRDLRTKFKGLDDIYVWHALAGAWGGVRP STHL SKITP
Sbjct: 364 -NNGNGN--YKGDDMGMKAFTRDLRTKFKGLDDIYVWHALAGAWGGVRPGSTHLKSKITP 423

Query: 432 CKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQASDLFDSMHSYLSKVGITGVKVDVIHTLE 491
            KSSPGLDGTMTDLAVVKVIEGKI LVHPDQA DL+DSMHSYLSK GITGVKVDVIHTLE
Sbjct: 424 LKSSPGLDGTMTDLAVVKVIEGKIALVHPDQAYDLYDSMHSYLSKAGITGVKVDVIHTLE 483

Query: 492 YVSEEYGGRVDLAKAYYKGLADSIVKNFKGTNLISSMQQCNDFFFLGTKQNSIGRVSDDF 551
           YV+EEYGGRVDLAK YYKGL DSIVKNFKGTNLISSMQQCNDFFFLGTKQNSIGRVSDDF
Sbjct: 484 YVAEEYGGRVDLAKVYYKGLTDSIVKNFKGTNLISSMQQCNDFFFLGTKQNSIGRVSDDF 543

Query: 552 WFHDPHGDPMGVYWLQGVHMVHCAFNSMWMGQIIQPDWDMFQSDHKCAKFHAGSRAICGG 611
           WFHDP GDP GVYWLQGVHMVHCA+NSMWMGQIIQPDWDMFQSDH CAKFHAGSRAICGG
Sbjct: 544 WFHDPQGDPTGVYWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDHMCAKFHAGSRAICGG 603

Query: 612 PVYVSDSVGGHDFDLIKKLAYPDGTIPRCQHFALPTRDCLFKNPLFDNKTVLKIWNLNKY 671
           PVYVSDSVGGHDFDLIKKLAYPDGTIPRCQHFALPTRDCLFKNPLFDNKTVLKIWNLNKY
Sbjct: 604 PVYVSDSVGGHDFDLIKKLAYPDGTIPRCQHFALPTRDCLFKNPLFDNKTVLKIWNLNKY 663

Query: 672 GGVIGAFNCQGAGWDRKEKRIKGRLECYKPMSTTVHVNDVEWDQKPEAAPMGNFVEYLVY 731
           GG+IGAFNCQGAGWDRKEKRIKG  ECYKP+STTVHVNDVEWDQKPE APMGN VEY+VY
Sbjct: 664 GGIIGAFNCQGAGWDRKEKRIKGHPECYKPISTTVHVNDVEWDQKPETAPMGNSVEYIVY 723

Query: 732 LNQAEQILLTTPKSEPLKVTLQPSTFEIFSFIPLRKLNSNIKFAPIGLTNMFNSSGTIQH 791
           LNQAE+IL TTPKS+PL++TLQPSTFE+F+F P++KL+SNIKFAPIGLTNMFNSSGTIQH
Sbjct: 724 LNQAEEILHTTPKSKPLELTLQPSTFELFNFTPIKKLDSNIKFAPIGLTNMFNSSGTIQH 783

Query: 792 LKYNDNGAELKVKGGGSFLAYSSGSPKKCLSNGIEVEFEWDSDGKLRLDLPWIEEGGGIS 851
           LKYN NG ELKVKGGG+FLAYSSGSPKKC+SNG+EVEFEWDS GKL LDLPW+EE GG+S
Sbjct: 784 LKYNKNGVELKVKGGGNFLAYSSGSPKKCISNGMEVEFEWDSKGKLSLDLPWMEEVGGVS 842

Query: 852 NLDIFF 856
           NLDI F
Sbjct: 844 NLDILF 842

BLAST of Spg014128 vs. ExPASy TrEMBL
Match: A0A6J1JSF9 (stachyose synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111487982 PE=3 SV=1)

HSP 1 Score: 1555.4 bits (4026), Expect = 0.0e+00
Identity = 742/849 (87.40%), Postives = 788/849 (92.82%), Query Frame = 0

Query: 10  LENHFDLSNGKINVKGVPLLSEVPSNVFFTPFSSIHQSSDAPLPLLQRVCGLSHKGGLLG 69
           L+N+  LSNG I+VKGVPLLSEVPSNVFFTPFSS+ +SSD PLPLLQRV  LSHKGG LG
Sbjct: 4   LDNYVHLSNGNIDVKGVPLLSEVPSNVFFTPFSSV-RSSDVPLPLLQRVRALSHKGGFLG 63

Query: 70  FD--QKQPSDRLMNSLGKFKGREFVSNFRFKTWWSTMWVGNSGSDLQMETQLVILNVPEI 129
           FD  Q QPSDRL+NSLGKF+ REFVS FRFKTWWSTMWVG+SGSD+QMETQ VILNVPEI
Sbjct: 64  FDQTQSQPSDRLINSLGKFESREFVSIFRFKTWWSTMWVGSSGSDIQMETQWVILNVPEI 123

Query: 130 RSYVVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYIHVSDNPYNLMKE 189
           RSYVVIIPIIEGSFRSALHPGTDGHVMIWAESGST VKASSFDAIAYIHVSDNPYNLMKE
Sbjct: 124 RSYVVIIPIIEGSFRSALHPGTDGHVMIWAESGSTHVKASSFDAIAYIHVSDNPYNLMKE 183

Query: 190 AYAAIRVHLNTFRLLEEKTVPTLVDRFGWCTWNAFFLNVNPVGVWNGVNDFAEGGISLRF 249
           AYAAIRVHLNTFRLLEEK+VP LVDRFGWCTWNAFFLNVNPVGVW+GVNDF EGG+SLRF
Sbjct: 184 AYAAIRVHLNTFRLLEEKSVPDLVDRFGWCTWNAFFLNVNPVGVWHGVNDFTEGGVSLRF 243

Query: 250 LIIDDGWQSINIDGEDPNQDMKYIVLGANQMTARLYKFEECDKFRKYKGGSLLGPNAPPF 309
           LIIDDGWQSIN DGEDP++DMK+IVLGANQMTARLYKFEECDKF KYKGG++LGPNAPPF
Sbjct: 244 LIIDDGWQSINADGEDPHRDMKHIVLGANQMTARLYKFEECDKFGKYKGGTMLGPNAPPF 303

Query: 310 DPKKPKLLISKAIEIVQAEKDRDNAIQSGVTDVSQFEAKVQQLKQELIDIFGEEEEEEES 369
           DPKKPKLLISK+IEI  AEKDRDN IQSGVTD+S+FEAKVQ+LKQEL+DIFG +++EEE 
Sbjct: 304 DPKKPKLLISKSIEIDNAEKDRDNVIQSGVTDISKFEAKVQKLKQELMDIFGNDQDEEE- 363

Query: 370 SVVSNGSGNCSYK-ADDFGMKAFTRDLRTKFKGLDDIYVWHALAGAWGGVRPDSTHLSSK 429
              +N + NC YK  DD GMKAFTRDLRTKFKGLDDIYVWHALAGAWGGVRP STHL SK
Sbjct: 364 ---NNRNENCCYKGGDDMGMKAFTRDLRTKFKGLDDIYVWHALAGAWGGVRPGSTHLKSK 423

Query: 430 ITPCKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQASDLFDSMHSYLSKVGITGVKVDVIH 489
           ITP KSSPGLDGTMTDLAVVKVIEGKIGLVHPDQA DL+DSMHSYLSK GITGVKVDVIH
Sbjct: 424 ITPLKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQAYDLYDSMHSYLSKAGITGVKVDVIH 483

Query: 490 TLEYVSEEYGGRVDLAKAYYKGLADSIVKNFKGTNLISSMQQCNDFFFLGTKQNSIGRVS 549
           TLEYVSEEYGGRVDLAK YYKGL DSI+KNFKGTNLISSMQQCNDFFFLGTKQNSIGR S
Sbjct: 484 TLEYVSEEYGGRVDLAKTYYKGLTDSIMKNFKGTNLISSMQQCNDFFFLGTKQNSIGRAS 543

Query: 550 DDFWFHDPHGDPMGVYWLQGVHMVHCAFNSMWMGQIIQPDWDMFQSDHKCAKFHAGSRAI 609
           DDFWFHDPHGDPMG +WLQGVHMVHCA+NSMWMGQIIQPDWDMFQSDH CAKFHAGSRA+
Sbjct: 544 DDFWFHDPHGDPMGEFWLQGVHMVHCAYNSMWMGQIIQPDWDMFQSDHVCAKFHAGSRAV 603

Query: 610 CGGPVYVSDSVGGHDFDLIKKLAYPDGTIPRCQHFALPTRDCLFKNPLFDNKTVLKIWNL 669
           CGGP+YVSDSVG HDFDLIKKL YPDGTIPRC+HFALPTRDCLFKNPLFDNKTVLKIWNL
Sbjct: 604 CGGPIYVSDSVGSHDFDLIKKLVYPDGTIPRCKHFALPTRDCLFKNPLFDNKTVLKIWNL 663

Query: 670 NKYGGVIGAFNCQGAGWDRKEKRIKGRLECYKPMSTTVHVNDVEWDQKPEAAPMGNFVEY 729
           NKYGGVIGAFNCQGAGWDRKE+RIKG  ECYKPMSTTVHVNDVEWDQKPE APMGNFVEY
Sbjct: 664 NKYGGVIGAFNCQGAGWDRKERRIKGHPECYKPMSTTVHVNDVEWDQKPETAPMGNFVEY 723

Query: 730 LVYLNQAEQILLTTPKSEPLKVTLQPSTFEIFSFIPLRKLNSNIKFAPIGLTNMFNSSGT 789
           +VYLNQA+QIL TTP SEPL++TLQPSTFE+F+FIPL KL SNIKFAPIGLTNMFNSSGT
Sbjct: 724 VVYLNQAKQILYTTPNSEPLELTLQPSTFELFNFIPLEKLGSNIKFAPIGLTNMFNSSGT 783

Query: 790 IQHLKYNDNGAELKVKGGGSFLAYSSGSPKKCLSNGIEVEFEWDSDGKLRLDLPWIEEGG 849
           IQHLKYN+N  ELKVKGGG+FLAYSSGSPKKC+SNG+EV FEWD DGKL  DL WIEE G
Sbjct: 784 IQHLKYNENDVELKVKGGGNFLAYSSGSPKKCVSNGMEVAFEWDYDGKLSFDLCWIEEAG 843

Query: 850 GISNLDIFF 856
           G+SNLDI F
Sbjct: 844 GVSNLDILF 847

BLAST of Spg014128 vs. ExPASy TrEMBL
Match: A0A1S3BRI8 (stachyose synthase OS=Cucumis melo OX=3656 GN=LOC103492745 PE=3 SV=1)

HSP 1 Score: 1520.4 bits (3935), Expect = 0.0e+00
Identity = 717/854 (83.96%), Postives = 783/854 (91.69%), Query Frame = 0

Query: 2   SSLLKPKSLENHFDLSNGKINVKGVPLLSEVPSNVFFTPFSSIHQSSDAPLPLLQRVCGL 61
           +S+LK   LEN  D S+GKI+VKGVP+LSEVP+NVFF+PFSSI QSSDAPLPLLQRV  L
Sbjct: 12  ASVLKSDGLENLIDFSDGKISVKGVPVLSEVPNNVFFSPFSSISQSSDAPLPLLQRVHSL 71

Query: 62  SHKGGLLGFDQKQPSDRLMNSLGKFKGREFVSNFRFKTWWSTMWVGNSGSDLQMETQLVI 121
           S+KGG LGFDQ QPSDRL NSLGKFKGREFVS FRFKTWWSTMWVGNSGSDLQMETQ V+
Sbjct: 72  SYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSGSDLQMETQWVM 131

Query: 122 LNVPEIRSYVVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYIHVSDNP 181
           LN+PEI+SYVVIIPIIEGSFRSALHPGTDG V+I AESGST VKASSFDAIAY+HVSDNP
Sbjct: 132 LNIPEIKSYVVIIPIIEGSFRSALHPGTDGQVLICAESGSTHVKASSFDAIAYVHVSDNP 191

Query: 182 YNLMKEAYAAIRVHLNTFRLLEEKTVPTLVDRFGWCTWNAFFLNVNPVGVWNGVNDFAEG 241
           Y LMKEAYAA+RVHLNTFRLLEEK V  LVD+FGWCTW+AF+L V+PVG+WNGVNDF EG
Sbjct: 192 YRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVGIWNGVNDFVEG 251

Query: 242 GISLRFLIIDDGWQSINIDGEDPNQDMKYIVLGANQMTARLYKFEECDKFRKYKGGSLLG 301
           GIS RFLIIDDGWQSIN+DGEDP +D K +VLG  QMTARLY F+EC+KFRKYKGGSL+G
Sbjct: 252 GISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYSFDECEKFRKYKGGSLMG 311

Query: 302 PNAPPFDPKKPKLLISKAIEIVQAEKDRDNAIQSGVTDVSQFEAKVQQLKQELIDIFGEE 361
           PNAP FDPKKPKLLI+KAIEI  AEK+RD AI SGVT+VS+FE K+Q+LK+EL++IFG  
Sbjct: 312 PNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKLKEELLEIFG-N 371

Query: 362 EEEEESSVVSNGSGNCSYKADDFGMKAFTRDLRTKFKGLDDIYVWHALAGAWGGVRPDST 421
           EEEEES+ ++ G  +CS KAD+ GMKAFTRDLRTKFKGLDDI+VWHALAGAWGGVRP +T
Sbjct: 372 EEEEESTAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAGAWGGVRPGAT 431

Query: 422 HLSSKITPCKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQASDLFDSMHSYLSKVGITGVK 481
           HL+SKI PCK SPGLDGTMTDLAVVK+IEG IGLVHPDQA D FDSMHSYLSKVGITGVK
Sbjct: 432 HLNSKIIPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSYLSKVGITGVK 491

Query: 482 VDVIHTLEYVSEEYGGRVDLAKAYYKGLADSIVKNFKGTNLISSMQQCNDFFFLGTKQNS 541
           VDV+HTLEYVSEEYGGRVDLAKAYYKGL +S++KNFKGT L SSMQQCNDFF+LGTKQNS
Sbjct: 492 VDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLLKNFKGTGLFSSMQQCNDFFYLGTKQNS 551

Query: 542 IGRVSDDFWFHDPHGDPMGVYWLQGVHMVHCAFNSMWMGQIIQPDWDMFQSDHKCAKFHA 601
           IGRV DDFWF DP+GDPMGVYWLQGVHM+HCA+NSMWMGQIIQPDWDMFQSDH CAKFHA
Sbjct: 552 IGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQSDHLCAKFHA 611

Query: 602 GSRAICGGPVYVSDSVGGHDFDLIKKLAYPDGTIPRCQHFALPTRDCLFKNPLFDNKTVL 661
           GSRAICGGPVYVSDSVGGH+FDLIK+L YPDGTIPRCQHFALPTRDCLFKNPLFDNKTVL
Sbjct: 612 GSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFKNPLFDNKTVL 671

Query: 662 KIWNLNKYGGVIGAFNCQGAGWDRKEKRIKGRLECYKPMSTTVHVNDVEWDQKPEAAPMG 721
           KIWNLNKYGGVIG FNCQGAGWD KE+RIKG  ECYKPMSTTVHVND+EWDQKPEAAPMG
Sbjct: 672 KIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDIEWDQKPEAAPMG 731

Query: 722 NFVEYLVYLNQAEQILLTTPKSEPLKVTLQPSTFEIFSFIPLRKLNSNIKFAPIGLTNMF 781
           NFVEY+VYLNQAEQI+ TTPKSEPLK T+QPSTFE+F+FIPLRKL SNIKFAPIGLTNMF
Sbjct: 732 NFVEYIVYLNQAEQIIHTTPKSEPLKATIQPSTFELFNFIPLRKLGSNIKFAPIGLTNMF 791

Query: 782 NSSGTIQHLKYNDNGAELKVKGGGSFLAYSSGSPKKCLSNGIEVEFEWDSDGKLRLDLPW 841
           NSSGTIQHLKYN+NG ELKVKGGG+FLAYSSGSPKKCLSNG E++F W+SDGKL  D+ W
Sbjct: 792 NSSGTIQHLKYNENGVELKVKGGGNFLAYSSGSPKKCLSNGTELKFVWNSDGKLSFDVHW 851

Query: 842 IEEGGGISNLDIFF 856
           IEE GGISNLDIFF
Sbjct: 852 IEEAGGISNLDIFF 864

BLAST of Spg014128 vs. TAIR 10
Match: AT4G01970.1 (stachyose synthase )

HSP 1 Score: 1061.6 bits (2744), Expect = 3.3e-310
Identity = 513/864 (59.38%), Postives = 653/864 (75.58%), Query Frame = 0

Query: 4   LLKPKSLENHFDLSNGKINVK-GVPLLSEVPSNVFFTPFSSIHQSSDAPLPLLQRVCGLS 63
           + KP    N F+LS G +  K   P+L +VP NV FTPFSS   S+DAPLP+L RV   +
Sbjct: 24  ITKPILQPNSFNLSEGSLCAKDSTPILFDVPQNVTFTPFSSHSISTDAPLPILLRVQANA 83

Query: 64  HKGGLLGFDQKQPSDRLMNSLGKFKGREFVSNFRFKTWWSTMWVGNSGSDLQMETQLVIL 123
           HKGG LGF ++ PSDRL NSLG+F+ REF+S FRFK WWST W+G SGSDLQ ETQ V+L
Sbjct: 84  HKGGFLGFTKESPSDRLTNSLGRFEDREFLSLFRFKMWWSTAWIGKSGSDLQAETQWVML 143

Query: 124 NVPEIRSYVVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYIHVSDNPY 183
            +PEI SYV IIP IEG+FR++L PG  G+V+I AESGST+VK SSF +IAYIH+ DNPY
Sbjct: 144 KIPEIDSYVAIIPTIEGAFRASLTPGEKGNVLICAESGSTKVKESSFKSIAYIHICDNPY 203

Query: 184 NLMKEAYAAIRVHLNTFRLLEEKTVPTLVDRFGWCTWNAFFLNVNPVGVWNGVNDFAEGG 243
           NLMKEA++A+RVH+NTF+LLEEK +P +VD+FGWCTW+A +L V+P  +W GV +F +GG
Sbjct: 204 NLMKEAFSALRVHMNTFKLLEEKKLPKIVDKFGWCTWDACYLTVDPATIWTGVKEFEDGG 263

Query: 244 ISLRFLIIDDGWQSINIDGEDPNQDMKYIVLGANQMTARLYKFEECDKFRKYKGGSLLGP 303
           +  +F+IIDDGWQSIN DG++ ++D + +VLG  QMTARL  F+EC KFR YKGGS +  
Sbjct: 264 VCPKFVIIDDGWQSINFDGDELDKDAENLVLGGEQMTARLTSFKECKKFRNYKGGSFITS 323

Query: 304 NAPPFDPKKPKLLISKAIEIVQAEKDRDNAI-QSGVTDVSQFEAKVQQLKQELIDIFGEE 363
           +A  F+P KPK+LI KA E +QA   R   + +SG  D+++ + K++ L +EL  +F E 
Sbjct: 324 DASHFNPLKPKMLIYKATERIQAIILRRKLVKESGEQDLTELDEKIKILSEELNAMFDEV 383

Query: 364 EEEEESSVVSNGSGNCSYKADDFGMKAFTRDLRTKFKGLDDIYVWHALAGAWGGVRPDS- 423
           E+EE     S GS + S      GM AFT+DLR +FK LDDIYVWHAL GAW GVRP++ 
Sbjct: 384 EKEE-----SLGSDDVS----GSGMAAFTKDLRLRFKSLDDIYVWHALCGAWNGVRPETM 443

Query: 424 THLSSKITPCKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQASDLFDSMHSYLSKVGITGV 483
             L +K+ P + SP L  TM DLAV KV+E  IGLVHP +A + +DSMHSYL+ VG+TG 
Sbjct: 444 MDLKAKVAPFELSPSLGATMADLAVDKVVEAGIGLVHPSKAHEFYDSMHSYLASVGVTGA 503

Query: 484 KVDVIHTLEYVSEEYGGRVDLAKAYYKGLADSIVKNFKGTNLISSMQQCNDFFFLGTKQN 543
           K+DV  TLE ++EE+GGRV+LAKAYY GL +S++KNF GT++I+SMQQCN+FFFL TKQ 
Sbjct: 504 KIDVFQTLESLAEEHGGRVELAKAYYDGLTESMIKNFNGTDVIASMQQCNEFFFLATKQI 563

Query: 544 SIGRVSDDFWFHDPHGDPMGVYWLQGVHMVHCAFNSMWMGQIIQPDWDMFQSDHKCAKFH 603
           SIGRV DDFW+ DP+GDP GVYWLQGVHM+HC++NS+WMGQ+IQPDWDMFQSDH CA++H
Sbjct: 564 SIGRVGDDFWWQDPYGDPQGVYWLQGVHMIHCSYNSIWMGQMIQPDWDMFQSDHVCAEYH 623

Query: 604 AGSRAICGGPVYVSDSVG--GHDFDLIKKLAYPDGTIPRCQHFALPTRDCLFKNPLFDNK 663
           A SRAICGGPVY+SD +G   H+FDLIKKLA+ DGTIPRC H+ALPTRD LFKNPLFD +
Sbjct: 624 AASRAICGGPVYLSDHLGKASHNFDLIKKLAFFDGTIPRCVHYALPTRDSLFKNPLFDKE 683

Query: 664 TVLKIWNLNKYGGVIGAFNCQGAGWDRKEKRIKGRLECYKPMSTTVHVNDVEWDQKPEAA 723
           ++LKI+N NK+GGVIG FNCQGAGW  +E R KG  ECY  +S TVHV+D+EWDQ PEAA
Sbjct: 684 SILKIFNFNKFGGVIGTFNCQGAGWSPEEHRFKGYKECYTTVSGTVHVSDIEWDQNPEAA 743

Query: 724 PMGNFV----EYLVYLNQAEQILLTTPKSEPLKVTLQPSTFEIFSFIPLRKL-NSNIKFA 783
             G+ V    +YLVY  Q+E+IL    KSE +K+TL+PS F++ SF+P+ +L +S ++FA
Sbjct: 744 --GSQVTYTGDYLVYKQQSEEILFMNSKSEAMKITLEPSAFDLLSFVPVTELVSSGVRFA 803

Query: 784 PIGLTNMFNSSGTIQHLKY-NDNGAELKVKGGGSFLAYSSGSPKKCLSNGIEVEFEWDSD 843
           P+GL NMFN  GT+Q +K   DN   + VKG G F+AYSS +P KC  N  E EF+W+ +
Sbjct: 804 PLGLINMFNCVGTVQDMKVTGDNSIRVDVKGEGRFMAYSSSAPVKCYLNDKEAEFKWEEE 863

Query: 844 -GKLRLDLPWIEEGGGISNLDIFF 856
            GKL   +PW+EE GGIS+L   F
Sbjct: 864 TGKLSFFVPWVEESGGISHLSFTF 876

BLAST of Spg014128 vs. TAIR 10
Match: AT5G40390.1 (Raffinose synthase family protein )

HSP 1 Score: 726.1 bits (1873), Expect = 3.3e-209
Identity = 373/854 (43.68%), Postives = 505/854 (59.13%), Query Frame = 0

Query: 14  FDLSNGKINVKGVPLLSEVPSNVFFTPFSSIHQSSDAPLPLLQRVCGLSHKGGLLGFD-Q 73
           F L +  +   G  +L++VP NV  T    +      PL +          G  +GF+  
Sbjct: 22  FRLEDSTLLANGQVVLTDVPVNVTLTSSPYLVDKDGVPLDV--------SAGSFIGFNLD 81

Query: 74  KQPSDRLMNSLGKFKGREFVSNFRFKTWWSTMWVGNSGSDLQMETQLVILNVP------- 133
            +P    + S+GK K   F+S FRFK WW+T WVG++G D++ ETQ++IL+         
Sbjct: 82  GEPKSHHVASIGKLKNIRFMSIFRFKVWWTTHWVGSNGRDIENETQIIILDQSGSDSGPG 141

Query: 134 --EIRSYVVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYIHVSDNPYN 193
               R YV+++P++EGSFRS+   G D  V +  ESGST+V  S F  I Y+H  D+P+ 
Sbjct: 142 SGSGRPYVLLLPLLEGSFRSSFQSGEDDDVAVCVESGSTEVTGSEFRQIVYVHAGDDPFK 201

Query: 194 LMKEAYAAIRVHLNTFRLLEEKTVPTLVDRFGWCTWNAFFLNVNPVGVWNGVNDFAEGGI 253
           L+K+A   IRVH+NTF+LLEEK+ P +VD+FGWCTW+AF+L VNP GV  GV    +GG 
Sbjct: 202 LVKDAMKVIRVHMNTFKLLEEKSPPGIVDKFGWCTWDAFYLTVNPDGVHKGVKCLVDGGC 261

Query: 254 SLRFLIIDDGWQSINIDGEDPNQDMKYIVLGANQMTARLYKFEECDKFRKYKGGSLLGPN 313
               ++IDDGWQSI  D +  + +   I +   QM  RL KFEE  KF+ Y         
Sbjct: 262 PPGLVLIDDGWQSIGHDSDGIDVEGMNITVAGEQMPCRLLKFEENHKFKDY--------- 321

Query: 314 APPFDPKKPKLLISKAIEIVQAEKDRDNAIQSGVTDVSQFEAKVQQLKQELIDIFGEEEE 373
                                + KD+                                  
Sbjct: 322 --------------------VSPKDQ---------------------------------- 381

Query: 374 EEESSVVSNGSGNCSYKADDFGMKAFTRDLRTKFKGLDDIYVWHALAGAWGGVRPDSTHL 433
                             +D GMKAF RDL+ +F  +D IYVWHAL G WGG+RP++  L
Sbjct: 382 ------------------NDVGMKAFVRDLKDEFSTVDYIYVWHALCGYWGGLRPEAPAL 441

Query: 434 -SSKITPCKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQASDLFDSMHSYLSKVGITGVKV 493
             S I   + SPGL  TM DLAV K+IE  IG   PD A + ++ +HS+L   GI GVKV
Sbjct: 442 PPSTIIRPELSPGLKLTMEDLAVDKIIETGIGFASPDLAKEFYEGLHSHLQNAGIDGVKV 501

Query: 494 DVIHTLEYVSEEYGGRVDLAKAYYKGLADSIVKNFKGTNLISSMQQCNDFFFLGTKQNSI 553
           DVIH LE + ++YGGRVDLAKAY+K L  S+ K+F G  +I+SM+ CNDF FLGT+  S+
Sbjct: 502 DVIHILEMLCQKYGGRVDLAKAYFKALTSSVNKHFNGNGVIASMEHCNDFMFLGTEAISL 561

Query: 554 GRVSDDFWFHDPHGDPMGVYWLQGVHMVHCAFNSMWMGQIIQPDWDMFQSDHKCAKFHAG 613
           GRV DDFW  DP GDP G +WLQG HMVHCA+NS+WMG  IQPDWDMFQS H CA+FHA 
Sbjct: 562 GRVGDDFWCTDPSGDPNGTFWLQGCHMVHCAYNSLWMGNFIQPDWDMFQSTHPCAEFHAA 621

Query: 614 SRAICGGPVYVSDSVGGHDFDLIKKLAYPDGTIPRCQHFALPTRDCLFKNPLFDNKTVLK 673
           SRAI GGP+Y+SD VG HDFDL+K+L  P+G+I RC+++ALPTRD LF++PL D KT+LK
Sbjct: 622 SRAISGGPIYISDCVGKHDFDLLKRLVLPNGSILRCEYYALPTRDRLFEDPLHDGKTMLK 681

Query: 674 IWNLNKYGGVIGAFNCQGAGWDRKEKRIKGRLECYKPMSTTVHVNDVEWDQKPEAAPMGN 733
           IWNLNKY GVIGAFNCQG GW R+ +R +   EC   ++ T    DVEW+       + N
Sbjct: 682 IWNLNKYTGVIGAFNCQGGGWCRETRRNQCFSECVNTLTATTSPKDVEWNSGSSPISIAN 741

Query: 734 FVEYLVYLNQAEQILLTTPKSEPLKVTLQPSTFEIFSFIPLRKLNSN-IKFAPIGLTNMF 793
             E+ ++L+Q++++LL +  ++ L++TL+P  FE+ +  P+  +  N ++FAPIGL NM 
Sbjct: 742 VEEFALFLSQSKKLLL-SGLNDDLELTLEPFKFELITVSPVVTIEGNSVRFAPIGLVNML 783

Query: 794 NSSGTIQHLKYNDNGAELKVKGGGSFLAYSSGSPKKCLSNGIEVEFEWDSDGKLRLDLPW 853
           N+SG I+ L YND   E+ V G G F  Y+S  P  CL +G  VEF ++ D  + + +PW
Sbjct: 802 NTSGAIRSLVYNDESVEVGVFGAGEFRVYASKKPVSCLIDGEVVEFGYE-DSMVMVQVPW 783

Query: 854 IEEGGGISNLDIFF 856
                G+S++   F
Sbjct: 862 -SGPDGLSSIQYLF 783

BLAST of Spg014128 vs. TAIR 10
Match: AT3G57520.1 (seed imbibition 2 )

HSP 1 Score: 454.5 bits (1168), Expect = 1.9e-127
Identity = 274/878 (31.21%), Postives = 424/878 (48.29%), Query Frame = 0

Query: 9   SLENHFDLSNGKINVKGVPLLSEVPSNVFFTPFSSIHQSSDAPLPLLQRVCGLSHKGGLL 68
           ++ ++  + N  + V+G  +L+++P N+  TP +     S                G  +
Sbjct: 2   TITSNISVQNDNLVVQGKTILTKIPDNIILTPVTGNGFVS----------------GSFI 61

Query: 69  GFDQKQPSDRLMNSLGKFKGREFVSNFRFKTWWSTMWVGNSGSDLQMETQLVIL------ 128
           G   +Q     +  +G  +G  F+  FRFK WW T  +G+ G D+ +ETQ ++L      
Sbjct: 62  GATFEQSKSLHVFPIGVLEGLRFMCCFRFKLWWMTQRMGSCGKDIPLETQFMLLESKDEV 121

Query: 129 -----NVPEIRSYVVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYIHV 188
                + P +  Y V +P++EG FR+ L       + I  ESG   V+ S    + Y+H 
Sbjct: 122 EGNGDDAPTV--YTVFLPLLEGQFRAVLQGNEKNEIEICFESGDKAVETSQGTHLVYVHA 181

Query: 189 SDNPYNLMKEAYAAIRVHLNTFRLLEEKTVPTLVDRFGWCTWNAFFLNVNPVGVWNGVND 248
             NP+ +++++  A+  H+ TF   E+K +P+ +D FGWCTW+AF+ +V   GV  G+  
Sbjct: 182 GTNPFEVIRQSVKAVERHMQTFHHREKKKLPSFLDWFGWCTWDAFYTDVTAEGVDEGLKS 241

Query: 249 FAEGGISLRFLIIDDGWQSINIDGEDPNQDMKYIVLGANQMTARLYKFEECDKFRKYKGG 308
            +EGG   +FLIIDDGWQ I    E+  +D   +V    Q   RL   +E  KF+K    
Sbjct: 242 LSEGGTPPKFLIIDDGWQQI----ENKEKDENCVVQEGAQFATRLVGIKENAKFQK---- 301

Query: 309 SLLGPNAPPFDPKKPKLLISKAIEIVQAEKDRDNAIQSGVTDVSQFEAKVQQLKQELIDI 368
                                       +KD         T VS  ++ V   KQ     
Sbjct: 302 --------------------------SDQKD---------TQVSGLKSVVDNAKQR---- 361

Query: 369 FGEEEEEEESSVVSNGSGNCSYKADDFGMKAFTRDLRTKFKGLDDIYVWHALAGAWGGVR 428
                                                     +  +Y WHALAG WGGV+
Sbjct: 362 ----------------------------------------HNVKQVYAWHALAGYWGGVK 421

Query: 429 PDST---HLSSKITPCKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQASDLFDSMHSYLSK 488
           P ++   H  S +     SPG+ G   D+ +  +    +GLV+P +  + ++ +HSYL+ 
Sbjct: 422 PAASGMEHYDSALAYPVQSPGVLGNQPDIVMDSLAVHGLGLVNPKKVFNFYNELHSYLAS 481

Query: 489 VGITGVKVDVIHTLEYVSEEYGGRVDLAKAYYKGLADSIVKNFKGTNLISSMQQCNDFFF 548
            GI GVKVDV + +E +    GGRV L ++Y + L  SI +NF     IS M    D  +
Sbjct: 482 CGIDGVKVDVQNIIETLGAGLGGRVSLTRSYQQALEASIARNFTDNGCISCMCHNTDGLY 541

Query: 549 LGTKQNSIGRVSDDFWFHDPHGDPMGVYWLQGVHMVHCAFNSMWMGQIIQPDWDMFQSDH 608
              KQ +I R SDDF+  DP            +H+   A+NS+++G+ +QPDWDMF S H
Sbjct: 542 -SAKQTAIVRASDDFYPRDPAS--------HTIHIASVAYNSLFLGEFMQPDWDMFHSLH 601

Query: 609 KCAKFHAGSRAICGGPVYVSDSVGGHDFDLIKKLAYPDGTIPRCQHFALPTRDCLFKNPL 668
             A++HA +RA+ G  +YVSD  G H+FDL++KL  PDG++ R +    PTRDCLF +P 
Sbjct: 602 PTAEYHAAARAVGGCAIYVSDKPGNHNFDLLRKLVLPDGSVLRAKLPGRPTRDCLFADPA 661

Query: 669 FDNKTVLKIWNLNKYGGVIGAFNCQGAGWDRKEKRIKGRLECYKPMSTTVHVNDVEWDQK 728
            D  ++LKIWN+NK+ G++G FNCQGAGW ++ K  K ++    P + T  +   + D  
Sbjct: 662 RDGISLLKIWNMNKFTGIVGVFNCQGAGWCKETK--KNQIHDTSPGTLTGSIRADDADLI 721

Query: 729 PEAAPMGNFVEYLVYLNQAEQILLTTPKSEPLKVTLQPSTFEIFSFIPLRKLNSNIKFAP 788
            + A      + +VY  ++ ++ +  PK   + +TL+   +E+F   PL+++  NI FAP
Sbjct: 722 SQVAGEDWSGDSIVYAYRSGEV-VRLPKGASIPLTLKVLEYELFHISPLKEITENISFAP 762

Query: 789 IGLTNMFNSSGTIQHLKYN----------------------DNGA-----ELKVKGGGSF 845
           IGL +MFNSSG I+ +  N                      DN +      + V+G G F
Sbjct: 782 IGLVDMFNSSGAIESIDINHVTDKNPEFFDGEISSASPALSDNRSPTALVSVSVRGCGRF 762

BLAST of Spg014128 vs. TAIR 10
Match: AT1G55740.1 (seed imbibition 1 )

HSP 1 Score: 445.7 bits (1145), Expect = 8.6e-125
Identity = 271/839 (32.30%), Postives = 411/839 (48.99%), Query Frame = 0

Query: 16  LSNGKINVKGVPLLSEVPSNVFFTPFSSIHQSSDAPLPLLQRVCGLSHKGGLLGFDQKQP 75
           +++  + V G  +L  VP NV  TP S                      G  +G    Q 
Sbjct: 9   VTDSDLVVLGHRVLHGVPENVLVTPASG----------------NALIDGAFIGVTSDQT 68

Query: 76  SDRLMNSLGKFKGREFVSNFRFKTWWSTMWVGNSGSDLQMETQLVILNV---------PE 135
               + SLGK +   F+  FRFK WW T  +G +G ++  ETQ +I+            +
Sbjct: 69  GSHRVFSLGKLEDLRFMCVFRFKLWWMTQRMGTNGKEIPCETQFLIVEANQGSDLGGRDQ 128

Query: 136 IRSYVVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYIHVSDNPYNLMK 195
             SYVV +PI+EG FR+ L       + I  ESG   V       + ++    +P++++ 
Sbjct: 129 SSSYVVFLPILEGDFRAVLQGNEANELEICLESGDPTVDQFEGSHLVFVAAGSDPFDVIT 188

Query: 196 EAYAAIRVHLNTFRLLEEKTVPTLVDRFGWCTWNAFFLNVNPVGVWNGVNDFAEGGISLR 255
           +A  A+  HL TF   E K +P +++ FGWCTW+AF+ NV    V  G+     GG++ +
Sbjct: 189 KAVKAVEQHLQTFSHRERKKMPDMLNWFGWCTWDAFYTNVTAKDVKQGLESLKAGGVTPK 248

Query: 256 FLIIDDGWQSINIDGEDPNQDMKYIVLGANQMTARLYKFEECDKFRKYKGGSLLGPNAPP 315
           F+IIDDGWQS+ +D       +++    A     RL   +E  KF+K             
Sbjct: 249 FVIIDDGWQSVGMD----ETSVEFNADNAANFANRLTHIKENHKFQK------------- 308

Query: 316 FDPKKPKLLISKAIEIVQAEKDRDNAIQSG--VTDVSQFEAKVQQLKQELIDIFGEEEEE 375
            D K             +  +  D ++  G  +TD+                        
Sbjct: 309 -DGK-------------EGHRVDDPSLSLGHVITDIK----------------------- 368

Query: 376 EESSVVSNGSGNCSYKADDFGMKAFTRDLRTKFKGLDDIYVWHALAGAWGGVRPDST--- 435
                 SN S                         L  +YVWHA+ G WGGV+P  +   
Sbjct: 369 ------SNNS-------------------------LKYVYVWHAITGYWGGVKPGVSGME 428

Query: 436 HLSSKITPCKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQASDLFDSMHSYLSKVGITGVK 495
           H  SK+    SSPG+  +     +  + +  +GLV+P++    ++ +HSYL+ VG+ GVK
Sbjct: 429 HYESKVAYPVSSPGVMSSENCGCLESITKNGLGLVNPEKVFSFYNDLHSYLASVGVDGVK 488

Query: 496 VDVIHTLEYVSEEYGGRVDLAKAYYKGLADSIVKNFKGTNLISSMQQCNDFFFLGTKQNS 555
           VDV + LE +   +GGRV LAK Y++ L  SI +NF    +IS M    D  +   K+ +
Sbjct: 489 VDVQNILETLGAGHGGRVKLAKKYHQALEASISRNFPDNGIISCMSHNTDGLY-SAKKTA 548

Query: 556 IGRVSDDFWFHDPHGDPMGVYWLQGVHMVHCAFNSMWMGQIIQPDWDMFQSDHKCAKFHA 615
           + R SDDFW  DP            +H+   A+N++++G+ +QPDWDMF S H  A++HA
Sbjct: 549 VIRASDDFWPRDPAS--------HTIHIASVAYNTLFLGEFMQPDWDMFHSLHPMAEYHA 608

Query: 616 GSRAICGGPVYVSDSVGGHDFDLIKKLAYPDGTIPRCQHFALPTRDCLFKNPLFDNKTVL 675
            +RA+ G  +YVSD  G HDF+L++KL   DG+I R +    PT DC F +P+ DNK++L
Sbjct: 609 AARAVGGCAIYVSDKPGQHDFNLLRKLVLRDGSILRAKLPGRPTSDCFFSDPVRDNKSLL 668

Query: 676 KIWNLNKYGGVIGAFNCQGAGWDRKEKRIKGRLECYKPMSTTVHVNDVEWDQKPEAAPMG 735
           KIWNLN++ GVIG FNCQGAGW + EKR     +    +S  V  NDV +  K  A    
Sbjct: 669 KIWNLNEFTGVIGVFNCQGAGWCKNEKRYLIHDQEPGTISGCVRTNDVHYLHKVAAFEWT 728

Query: 736 NFVEYLVYLNQAEQILLTTPKSEPLKVTLQPSTFEIFSFIPLRKLNSNIKFAPIGLTNMF 795
              + +VY +   + L+  PK   L VTL P  +E+F+ +P+++ +   KFAP+GL  MF
Sbjct: 729 G--DSIVYSHLRGE-LVYLPKDTSLPVTLMPREYEVFTVVPVKEFSDGSKFAPVGLMEMF 734

Query: 796 NSSGTIQHLKYNDNGAE----LKVKGGGSFLAYSS-GSPKKCLSNGIEVEFEWDSDGKL 836
           NS G I  L+Y+D G +    +K++G G    YSS   P+    +  +VE+ ++ +  L
Sbjct: 789 NSGGAIVSLRYDDEGTKFVVRMKLRGSGLVGVYSSVRRPRSVTVDSDDVEYRYEPESGL 734

BLAST of Spg014128 vs. TAIR 10
Match: AT5G20250.1 (Raffinose synthase family protein )

HSP 1 Score: 445.7 bits (1145), Expect = 8.6e-125
Identity = 273/837 (32.62%), Postives = 400/837 (47.79%), Query Frame = 0

Query: 16  LSNGKINVKGVPLLSEVPSNVFFTPFSSIHQSSDAPLPLLQRVCGLSHKGGLLGFDQKQP 75
           +S+G + +K   +L+ VP NV  T       S   P+           +G  +G    + 
Sbjct: 9   ISDGNLIIKNRTILTGVPDNVITT-----SASEAGPV-----------EGVFVGAVFNKE 68

Query: 76  SDRLMNSLGKFKGREFVSNFRFKTWWSTMWVGNSGSDLQMETQLVILNVPE--------- 135
             + +  +G  +   F+S FRFK WW    +G  G D+  ETQ +++   +         
Sbjct: 69  ESKHIVPIGTLRNSRFMSCFRFKLWWMAQRMGEMGRDIPYETQFLLVESNDGSHLESDGA 128

Query: 136 ------IRSYVVIIPIIEGSFRSALHPGTDGHVMIWAESGSTQVKASSFDAIAYIHVSDN 195
                  + Y V +P+IEGSFRS L    +  V +  ESG    K SSF    YIH   +
Sbjct: 129 NGVECNQKVYTVFLPLIEGSFRSCLQGNVNDEVELCLESGDVDTKRSSFTHSLYIHAGTD 188

Query: 196 PYNLMKEAYAAIRVHLNTFRLLEEKTVPTLVDRFGWCTWNAFFLNVNPVGVWNGVNDFAE 255
           P+  + +A   +++HLN+FR   EK +P +VD FGWCTW+AF+  V   GV  G+   A 
Sbjct: 189 PFQTITDAIRTVKLHLNSFRQRHEKKLPGIVDYFGWCTWDAFYQEVTQEGVEAGLKSLAA 248

Query: 256 GGISLRFLIIDDGWQSINIDGEDPNQDMKYIVLGANQMTARLYKFEECDKFRKYKGGSLL 315
           GG   +F+IIDDGWQS+  D      D K           RL   +E +KF+K       
Sbjct: 249 GGTPPKFVIIDDGWQSVERDATVEAGDEK-----KESPIFRLTGIKENEKFKK------- 308

Query: 316 GPNAPPFDPKKPKLLISKAIEIVQAEKDRDNAIQSGVTDVSQFEAKVQQLKQELIDIFGE 375
                                     KD  N                             
Sbjct: 309 --------------------------KDDPNV---------------------------- 368

Query: 376 EEEEEESSVVSNGSGNCSYKADDFGMKAFTRDLRTKFKGLDDIYVWHALAGAWGGVRPDS 435
                                   G+K   +  + K  GL  +YVWHA+ G WGGVRP  
Sbjct: 369 ------------------------GIKNIVKIAKEK-HGLKYVYVWHAITGYWGGVRPGE 428

Query: 436 THLSSKITPCKSSPGLDGTMTDLAVVKVIEGKIGLVHPDQASDLFDSMHSYLSKVGITGV 495
            + S    P  S   ++   T    V  ++G +GLV P +    ++ +HSYL+  G+ GV
Sbjct: 429 EYGSVMKYPNMSKGVVENDPTWKTDVMTLQG-LGLVSPKKVYKFYNELHSYLADAGVDGV 488

Query: 496 KVDVIHTLEYVSEEYGGRVDLAKAYYKGLADSIVKNFKGTNLISSMQQCNDFFFLGTKQN 555
           KVDV   LE +    GGRV+L + +++ L  S+ KNF     I+ M    D  +  +KQ 
Sbjct: 489 KVDVQCVLETLGGGLGGRVELTRQFHQALDSSVAKNFPDNGCIACMSHNTDALYC-SKQA 548

Query: 556 SIGRVSDDFWFHDPHGDPMGVYWLQGVHMVHCAFNSMWMGQIIQPDWDMFQSDHKCAKFH 615
           ++ R SDDF+  DP            +H+   A+NS+++G+ +QPDWDMF S H  A++H
Sbjct: 549 AVIRASDDFYPRDPVS--------HTIHIASVAYNSVFLGEFMQPDWDMFHSVHPAAEYH 608

Query: 616 AGSRAICGGPVYVSDSVGGHDFDLIKKLAYPDGTIPRCQHFALPTRDCLFKNPLFDNKTV 675
           A +RAI GGP+YVSDS G H+F+L++KL  PDG+I R +    PTRDCLF +P  D  ++
Sbjct: 609 ASARAISGGPLYVSDSPGKHNFELLRKLVLPDGSILRARLPGRPTRDCLFADPARDGVSL 668

Query: 676 LKIWNLNKYGGVIGAFNCQGAGWDRKEKRIKGRLECYKPMSTTVHVNDVEWDQKPEAAPM 735
           LKIWN+NKY GV+G +NCQGA W   E++          ++ ++   DV    +    P 
Sbjct: 669 LKIWNMNKYTGVLGVYNCQGAAWSSTERKNIFHQTKTDSLTGSIRGRDVHSISEASTDPT 727

Query: 736 GNFVEYLVYLNQAEQILLTTPKSEPLKVTLQPSTFEIFSFIPLRKLNSNIKFAPIGLTNM 795
               +  VY +Q+   L+  P +  L V+L+    EIF+  P+  L   + FAPIGL NM
Sbjct: 729 TWNGDCAVY-SQSRGELIVMPYNVSLPVSLKIREHEIFTVSPISHLVDGVSFAPIGLVNM 727

Query: 796 FNSSGTIQHLKYNDNGAE--LKVKGGGSFLAYSSGSPKKCLSNGIEVEFEWDSDGKL 836
           +NS G I+ L+Y     +  ++VKG G F +YSS  PK+C+    E+ FE+DS   L
Sbjct: 789 YNSGGAIEGLRYEAEKMKVVMEVKGCGKFGSYSSVKPKRCVVESNEIAFEYDSSSGL 727