Spg014118 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg014118
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionTP_methylase domain-containing protein
Locationscaffold3: 46552235 .. 46555777 (+)
RNA-Seq ExpressionSpg014118
SyntenySpg014118
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTATAATATTGACAACAAGTAAGTTATATCAAACTCTAAAAAAAAATTTCAACACAAGGATCTCAAATGGTAATAATTTGAGAAGGACCTCAAATTACATTTGAATTCTTTCAGAAAAAAAGTGCATCACTTTAAATCTAAATTAAAAAAATATATATATTAAAAAAAAAAAAAAAAAAAAAAAAAAAACCACCAAAATCCCCAAGTTTCCAATTCACTCAAGCTAGTCCATAGAAATACCAAGTTGTCAAATTCATGGAAGCCAATAGCCAGTCCAAAGGAATTTCAAGCTGGTCTCTCACACATCTCAAAAGGCCACTCTTTTTTGCCTTCTCATCTTCCTCAAAGTCCATGTATTAGAACGACGAGCCGCCTCTTATTTATCCCATATTTATTCCAACAATATTCCCCTTACAACATCGCGTTTTTCCCTCTTCTCCTTCGCCATGGCTCGTGTTTACGAGCTTCAATCGCTTTCATCTCGATTTTCATCTCGCCCAACAGTACCCAGGTCCCCAAATTTCAAACCCATTTGCTCATTTCACTGCAGCTCCTCTTCTTCCTCTTCTTCTTCCCCATTTACAGAAAAACACTCCGTCAAGAGATACCAAAGAGACGATTGGCTGTACAAGAACCAAGCGGACCAAAGTTCGATTGCTCCATCGTGTTCTATTCCATGTGATTCTGAGTCCATACGGCAGAACGACATTGCCTTGCAGCTGCCGGAGCTGAAGAAATTGCTGCAGGTGCTGAGAGAAAAGAGGATAAATAGTGGTTGCGATGATGGAAAATGTGGGCCTGGGAATGTGTTTCTGGTGGGGACTGGCCCTGGAGATCCAGAGCTTTTGACATTGAAGGCGGTGAAAGTGATTCAGAGTGCTGATTTGCTCTTGTATGATCGATTGGTCTCTAATGATGTGTTGGATTTGGTGGGTCCTGATGCTAGGCTTCTCTATGTGGGCAAGACTGCAGGTTACCATAGCAGAACCCAGGTGAGGATTTTCTTCTGAGAAATCTCTAGTCATGAGGGTTCTTTATAAATATCTATCTGGGTTTTGCATTTTTAACATTCTTGTGAAAAGGGTAGAGTGTTAAGTGTTTGAGTTTTGTTGCTGAATGGGGATTGTTTTAGGAGGAGATTCATGAGTTACTTCTGAACTTTGCTGAAGCTGGAGCTACAGTTGTGAGGCTTAAAGGAGGAGACCCTCTTGTAAGTGTATATTTTGGATGGTTGAAGTGTAATTGTGAATATGGTCTTGTCATTCAAGTGATTTTTCGTGTTGTCATCCTGAAAGTGTTATCGTTGTATTTTGTAGGTGTTTGGAAGGGGTGGGGAGGAGATGGATTTCTTGCAACAACAGGGTATTCAAGTAAAAATCGTCCCTGGTAAGTCTATTTCTGAATGCAGTTACACTAATACAATCCTTTGAGAGTTTCAATGAAGCTTAATTGAATGTGTTCTGCATCACCATTTGTTAGAAAGATTTGTTTGTTTAATGGTGCCATCATGACTAAAAGCCAACTGTACTAGTGCATGTGAATATTTGCAGTTCTGAGCACTTGTTCCTAGTGTCTTCAAGTGACTCCAATGTATGCAAATTCCTGTTTGTGAAGTTTGTACTCGATATCCTGAGTACTTTAGTTATGCTAGTGTATGGACCTGAACTATGGTGCCCTTATTTCTCTTTGAACCATATTAAGTTAGAATGTAACACCTCCTGTTTGAGATGGAAAATTTCGTAGGGAAGGAAAAAATGCTTGTAGGCCCTCGCTCCAAATGGGTCATTACATTCTAACACCCTGATGGAGAGAATTTCCCACCCTGACCTGTCCCCAACAGAGAGAATGGAATGACATTAAGTCATGTGGGATAGAACCAGTAGGAGTAAGCTTGATTGAAATGGGGTTTTAGAATGGGAAATTAGTTTTGCTAATCTGTCAACAGCCACAAGTAACACGAACTGTCATAAGTATATTACATTTCTCATTCCCAATGTCATGTCTTTGTCTATGCATCACCATTTTAAGGAAACTACTATTAGAGTAACCTCATATTAAAAGATGTGTCTATGCCTTGTAGGTATAACTGCTGCTTCAGGCATATCAGCTGAATTGGGGATTCCTTTAACACACAGGGGCGTCGCAACAAGCGTTAGGTTCCTTACTGGTCACTCTAGGAAGGGTGGAACCGATCCTCTATATGTAGCAGAAAATGCAGCTGATCCAGATTCAACTCTGGTGGTATATATGGGTCTGTCAACTCTTCCATCTCTGGCCCTTAAGTTGATGCATCATGGTCTGCCCCCCGATACCCCGGCTGCTGCCGTTGAACGAGGAACAACACCCCAACAAAGAACTGTAAGCACAATTCTCTGCCACCTTCTACCTTGTCCTTGGTTTGACCATATAATCGGTTTCATAAAAAAAAGTCTCATGAACTCTTAATGCCTTGGTGCATTACCATTCTACTTAATCCAGGAGATCTCTCATATGTCTTTTTTCTTATCTGTGCTGGATTCTGTACCTCATTAGCTACTTTTTGTGAACATCCGGTTCTAGACATTTATATTGAGGGGGCTGGACATGATTACACTTCATTTCATTTTGCCAGTACCTATAACGAATCTTGCTTGGATCTATTACAATTATGCAGGTTTTTGCAGAACTGAAGGACCTCGCAGATGAAATCAAAGCAGCAGAGTTGGTTTCTCCTACTTTAATCATAATTGGAAAAGTGGTTTCTCTCTCACCACATTGGTCTCTTTCTTCCAAAGATGCCTCCTATTTGGTGGAGGCTTCATAGAATTAAAGAGCCTAGAAAAGGTTATGTAAACCGGGCATAGTTCTTCATTCTGACACTTAACGATTACTTCGAGGAGCAACCGAGATAAAAAGCTCGGGTTGTCGAGTTCTTCCACAAGATTTTATCGATTTTGTGGAGAAAACATTGAAACCATGACAGAATAGAAAAAGAAGAATTGGGGACTCTCCTGTCCCTTCTACATTTGATTGGTCTGCCCATTGCATTAGAACGATTGGATTCAGACTTTTTCTGAAGGATTGGACTGATGCAGCGATTTTCTTTCTCAGATGCTACCGGAGCTCAGTTAGCAGCATGTTCTTGTAATCTCGTACCATTTCTTGCATTGATTCAAATAAAAGTTCCAAACTTCAATTTTTTCTTAGTTCATGAGGATTCTGGATTTCTGGTGCGGTGAGAATACTTCATCAGGGACCAATTTTGTTCACGAAGTCTTCTTGTTGATAAAGTAGTTGGTGAAAATTTTTGCAGAACTTTCCTTGGAATATCTCATGTATTGATTAAACTCTGTTTTTGTTTCTTTTGTCCCCTTAATTACTTCCAGTTCTAGAATTTTCTTTATCTTTCGAGTAAAAGTTTTAGAACTAACAAAAAAAGCATGTGTGGAAATTCACCTCAAACATGACTCTGTAGTTCCTGCAGACTGGGATTTATCTCTCTGACATATAATTTGGATAAAAATGTTGGTTTGATTAAGAGCATTTTGGAAGTGTCTGATGTTT

mRNA sequence

ATGGCTCGTGTTTACGAGCTTCAATCGCTTTCATCTCGATTTTCATCTCGCCCAACAGTACCCAGGTCCCCAAATTTCAAACCCATTTGCTCATTTCACTGCAGCTCCTCTTCTTCCTCTTCTTCTTCCCCATTTACAGAAAAACACTCCGTCAAGAGATACCAAAGAGACGATTGGCTGTACAAGAACCAAGCGGACCAAAGTTCGATTGCTCCATCGTGTTCTATTCCATGTGATTCTGAGTCCATACGGCAGAACGACATTGCCTTGCAGCTGCCGGAGCTGAAGAAATTGCTGCAGGTGCTGAGAGAAAAGAGGATAAATAGTGGTTGCGATGATGGAAAATGTGGGCCTGGGAATGTGTTTCTGGTGGGGACTGGCCCTGGAGATCCAGAGCTTTTGACATTGAAGGCGGTGAAAGTGATTCAGAGTGCTGATTTGCTCTTGTATGATCGATTGGTCTCTAATGATGTGTTGGATTTGGTGGGTCCTGATGCTAGGCTTCTCTATGTGGGCAAGACTGCAGGTTACCATAGCAGAACCCAGGAGGAGATTCATGAGTTACTTCTGAACTTTGCTGAAGCTGGAGCTACAGTTGTGAGGCTTAAAGGAGGAGACCCTCTTGTGTTTGGAAGGGGTGGGGAGGAGATGGATTTCTTGCAACAACAGGGTATTCAAGTAAAAATCGTCCCTGGTATAACTGCTGCTTCAGGCATATCAGCTGAATTGGGGATTCCTTTAACACACAGGGGCGTCGCAACAAGCGTTAGGTTCCTTACTGGTCACTCTAGGAAGGGTGGAACCGATCCTCTATATGTAGCAGAAAATGCAGCTGATCCAGATTCAACTCTGGTGGTATATATGGGTCTGTCAACTCTTCCATCTCTGGCCCTTAAGTTGATGCATCATGGTCTGCCCCCCGATACCCCGGCTGCTGCCGTTGAACGAGGAACAACACCCCAACAAAGAACTGTTTTTGCAGAACTGAAGGACCTCGCAGATGAAATCAAAGCAGCAGAGTTGGTTTCTCCTACTTTAATCATAATTGGAAAAGTGGTTTCTCTCTCACCACATTGGTCTCTTTCTTCCAAAGATGCCTCCTATTTGGTGGAGGCTTCATAG

Coding sequence (CDS)

ATGGCTCGTGTTTACGAGCTTCAATCGCTTTCATCTCGATTTTCATCTCGCCCAACAGTACCCAGGTCCCCAAATTTCAAACCCATTTGCTCATTTCACTGCAGCTCCTCTTCTTCCTCTTCTTCTTCCCCATTTACAGAAAAACACTCCGTCAAGAGATACCAAAGAGACGATTGGCTGTACAAGAACCAAGCGGACCAAAGTTCGATTGCTCCATCGTGTTCTATTCCATGTGATTCTGAGTCCATACGGCAGAACGACATTGCCTTGCAGCTGCCGGAGCTGAAGAAATTGCTGCAGGTGCTGAGAGAAAAGAGGATAAATAGTGGTTGCGATGATGGAAAATGTGGGCCTGGGAATGTGTTTCTGGTGGGGACTGGCCCTGGAGATCCAGAGCTTTTGACATTGAAGGCGGTGAAAGTGATTCAGAGTGCTGATTTGCTCTTGTATGATCGATTGGTCTCTAATGATGTGTTGGATTTGGTGGGTCCTGATGCTAGGCTTCTCTATGTGGGCAAGACTGCAGGTTACCATAGCAGAACCCAGGAGGAGATTCATGAGTTACTTCTGAACTTTGCTGAAGCTGGAGCTACAGTTGTGAGGCTTAAAGGAGGAGACCCTCTTGTGTTTGGAAGGGGTGGGGAGGAGATGGATTTCTTGCAACAACAGGGTATTCAAGTAAAAATCGTCCCTGGTATAACTGCTGCTTCAGGCATATCAGCTGAATTGGGGATTCCTTTAACACACAGGGGCGTCGCAACAAGCGTTAGGTTCCTTACTGGTCACTCTAGGAAGGGTGGAACCGATCCTCTATATGTAGCAGAAAATGCAGCTGATCCAGATTCAACTCTGGTGGTATATATGGGTCTGTCAACTCTTCCATCTCTGGCCCTTAAGTTGATGCATCATGGTCTGCCCCCCGATACCCCGGCTGCTGCCGTTGAACGAGGAACAACACCCCAACAAAGAACTGTTTTTGCAGAACTGAAGGACCTCGCAGATGAAATCAAAGCAGCAGAGTTGGTTTCTCCTACTTTAATCATAATTGGAAAAGTGGTTTCTCTCTCACCACATTGGTCTCTTTCTTCCAAAGATGCCTCCTATTTGGTGGAGGCTTCATAG

Protein sequence

MARVYELQSLSSRFSSRPTVPRSPNFKPICSFHCSSSSSSSSSPFTEKHSVKRYQRDDWLYKNQADQSSIAPSCSIPCDSESIRQNDIALQLPELKKLLQVLREKRINSGCDDGKCGPGNVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGISAELGIPLTHRGVATSVRFLTGHSRKGGTDPLYVAENAADPDSTLVVYMGLSTLPSLALKLMHHGLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSSKDASYLVEAS
Homology
BLAST of Spg014118 vs. NCBI nr
Match: KAG6575668.1 (hypothetical protein SDJN03_26307, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 680.6 bits (1755), Expect = 7.5e-192
Identity = 350/372 (94.09%), Postives = 361/372 (97.04%), Query Frame = 0

Query: 1   MARVYELQSLSSRFSSRPTVPRSPNFKPICSFHCSSSSSSSSSPFTEKHSVKRYQRDDWL 60
           MARV +LQSLSS FSSRPT+PRS NFKPICSFHC SSSSSSSSPFTEKHSVKRYQRDDWL
Sbjct: 1   MARVCDLQSLSSLFSSRPTIPRSSNFKPICSFHC-SSSSSSSSPFTEKHSVKRYQRDDWL 60

Query: 61  YKNQADQSSIAPSCSIPCDSESIRQNDIALQLPELKKLLQVLREKRINSGCDDGKCGPGN 120
           YKNQAD+SS+A SCSIP DSESIRQNDIALQLPELKKLLQVLREKR +SG DDGKCGPGN
Sbjct: 61  YKNQADESSVASSCSIPSDSESIRQNDIALQLPELKKLLQVLREKRPSSGGDDGKCGPGN 120

Query: 121 VFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHSR 180
           VFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVG DARLLYVGKTAG+HSR
Sbjct: 121 VFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGFHSR 180

Query: 181 TQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIS 240
           TQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGI+
Sbjct: 181 TQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIA 240

Query: 241 AELGIPLTHRGVATSVRFLTGHSRKGGTDPLYVAENAADPDSTLVVYMGLSTLPSLALKL 300
           AELGIPLTHRGVATSVRFLTGHSR+GGTDPL+VAENAADPDSTLVVYMGLSTLPSLALKL
Sbjct: 241 AELGIPLTHRGVATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKL 300

Query: 301 MHHGLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWS 360
           MHHGLPPDTPAAA+ERGTTPQQR VFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWS
Sbjct: 301 MHHGLPPDTPAAAIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWS 360

Query: 361 LSSKDASYLVEA 373
           LSSK+AS LVEA
Sbjct: 361 LSSKEASSLVEA 371

BLAST of Spg014118 vs. NCBI nr
Match: XP_022954326.1 (uncharacterized protein LOC111456604 [Cucurbita moschata])

HSP 1 Score: 680.6 bits (1755), Expect = 7.5e-192
Identity = 350/375 (93.33%), Postives = 362/375 (96.53%), Query Frame = 0

Query: 1   MARVYELQSLSSRFSSRPTVPRSPNFKPICSFHC---SSSSSSSSSPFTEKHSVKRYQRD 60
           MARV +LQSLSS F+SRPT+PRS NFKPICSFHC   SSSSSSSSSPFTEKHSVKRYQRD
Sbjct: 1   MARVCDLQSLSSLFASRPTIPRSSNFKPICSFHCSSSSSSSSSSSSPFTEKHSVKRYQRD 60

Query: 61  DWLYKNQADQSSIAPSCSIPCDSESIRQNDIALQLPELKKLLQVLREKRINSGCDDGKCG 120
           DWLYKNQAD+SS+A SCSIP DSESIRQNDIALQLPELKKLLQVLREKR +SG DDGKCG
Sbjct: 61  DWLYKNQADESSVASSCSIPSDSESIRQNDIALQLPELKKLLQVLREKRASSGGDDGKCG 120

Query: 121 PGNVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGY 180
           PGNVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVG DARLLYVGKTAG+
Sbjct: 121 PGNVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGF 180

Query: 181 HSRTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAAS 240
           HSRTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAAS
Sbjct: 181 HSRTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAAS 240

Query: 241 GISAELGIPLTHRGVATSVRFLTGHSRKGGTDPLYVAENAADPDSTLVVYMGLSTLPSLA 300
           GI+AELGIPLTHRGVATSVRFLTGHSR+GGTDPL+VAENAADPDSTLVVYMGLSTLPSLA
Sbjct: 241 GIAAELGIPLTHRGVATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSLA 300

Query: 301 LKLMHHGLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSP 360
           LKLMHHGLPPDTPAAA+ERGTTPQQR VFAELKDLADEIKAAELVSPTLIIIGKVVSLSP
Sbjct: 301 LKLMHHGLPPDTPAAAIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSLSP 360

Query: 361 HWSLSSKDASYLVEA 373
           HWSLSSK+AS LVEA
Sbjct: 361 HWSLSSKEASSLVEA 375

BLAST of Spg014118 vs. NCBI nr
Match: XP_022991905.1 (uncharacterized protein LOC111488402 [Cucurbita maxima])

HSP 1 Score: 680.6 bits (1755), Expect = 7.5e-192
Identity = 347/372 (93.28%), Postives = 359/372 (96.51%), Query Frame = 0

Query: 1   MARVYELQSLSSRFSSRPTVPRSPNFKPICSFHCSSSSSSSSSPFTEKHSVKRYQRDDWL 60
           MARV +LQSLSS FSSRPT+PRS NFKPICSFHC    SSSSSPFTEKHSVKRYQRDDW+
Sbjct: 1   MARVCDLQSLSSLFSSRPTIPRSSNFKPICSFHC----SSSSSPFTEKHSVKRYQRDDWV 60

Query: 61  YKNQADQSSIAPSCSIPCDSESIRQNDIALQLPELKKLLQVLREKRINSGCDDGKCGPGN 120
           YKNQAD+SS+A SCSI CDSESIRQNDIALQLPELKKLLQVLREKR +SGCDDGKCGPGN
Sbjct: 61  YKNQADESSVASSCSISCDSESIRQNDIALQLPELKKLLQVLREKRASSGCDDGKCGPGN 120

Query: 121 VFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHSR 180
           VFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVG DARLLYVGKTAG+HSR
Sbjct: 121 VFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGFHSR 180

Query: 181 TQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIS 240
           TQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGI+
Sbjct: 181 TQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIA 240

Query: 241 AELGIPLTHRGVATSVRFLTGHSRKGGTDPLYVAENAADPDSTLVVYMGLSTLPSLALKL 300
           AELGIPLTHRGVATSVRFLTGHSR+GGTDPL+VAENAADPDSTLVVYMGLSTLPSLALKL
Sbjct: 241 AELGIPLTHRGVATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKL 300

Query: 301 MHHGLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWS 360
           MHHGLPPDTPAAA+ERGTTPQQR VFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWS
Sbjct: 301 MHHGLPPDTPAAAIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWS 360

Query: 361 LSSKDASYLVEA 373
           LSSK+AS LVEA
Sbjct: 361 LSSKEASSLVEA 368

BLAST of Spg014118 vs. NCBI nr
Match: XP_008461254.1 (PREDICTED: uroporphyrinogen-III C-methyltransferase [Cucumis melo])

HSP 1 Score: 680.6 bits (1755), Expect = 7.5e-192
Identity = 343/372 (92.20%), Postives = 359/372 (96.51%), Query Frame = 0

Query: 1   MARVYELQSLSSRFSSRPTVPRSPNFKPICSFHCSSSSSSSSSPFTEKHSVKRYQRDDWL 60
           MAR  +LQSLSS FSS PT+PRSPNFKPI SFHCS SSSSSSSPFTEKHS+KRYQRDDWL
Sbjct: 1   MARFCDLQSLSSPFSSHPTIPRSPNFKPIFSFHCSYSSSSSSSPFTEKHSIKRYQRDDWL 60

Query: 61  YKNQADQSSIAPSCSIPCDSESIRQNDIALQLPELKKLLQVLREKRINSGCDDGKCGPGN 120
           YKNQ+DQ S+  SCSIP DSES+RQNDIA+QLPELKKLL+VLREKR++SGCDDGKCGPGN
Sbjct: 61  YKNQSDQPSVTSSCSIPYDSESMRQNDIAMQLPELKKLLEVLREKRVSSGCDDGKCGPGN 120

Query: 121 VFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHSR 180
           VFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVL+LVGPDARLLYVGKTAGYHSR
Sbjct: 121 VFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLELVGPDARLLYVGKTAGYHSR 180

Query: 181 TQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIS 240
           TQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGI+
Sbjct: 181 TQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIA 240

Query: 241 AELGIPLTHRGVATSVRFLTGHSRKGGTDPLYVAENAADPDSTLVVYMGLSTLPSLALKL 300
           AELGIPLTHRGVATSVRFLTGHSRKGGTDPL+VAENAADPDSTLVVYMGLSTLPSLALKL
Sbjct: 241 AELGIPLTHRGVATSVRFLTGHSRKGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKL 300

Query: 301 MHHGLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWS 360
           MHHGLPPDTPAAAVERGTTPQQRTVFA LKDLADEIKAAELVSPTLI+IG+VVSLSPHWS
Sbjct: 301 MHHGLPPDTPAAAVERGTTPQQRTVFAHLKDLADEIKAAELVSPTLIVIGRVVSLSPHWS 360

Query: 361 LSSKDASYLVEA 373
           LSS +AS LVEA
Sbjct: 361 LSSNEASSLVEA 372

BLAST of Spg014118 vs. NCBI nr
Match: XP_023548332.1 (uncharacterized protein LOC111807000 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 679.9 bits (1753), Expect = 1.3e-191
Identity = 347/372 (93.28%), Postives = 359/372 (96.51%), Query Frame = 0

Query: 1   MARVYELQSLSSRFSSRPTVPRSPNFKPICSFHCSSSSSSSSSPFTEKHSVKRYQRDDWL 60
           MARV +LQSLSS F+SRPT+PRS NFKPICSFHC    SSSSSPFTEKHSVKRYQRDDWL
Sbjct: 1   MARVCDLQSLSSLFASRPTIPRSSNFKPICSFHC----SSSSSPFTEKHSVKRYQRDDWL 60

Query: 61  YKNQADQSSIAPSCSIPCDSESIRQNDIALQLPELKKLLQVLREKRINSGCDDGKCGPGN 120
           YKNQAD+SS+A SCSIP DSESIRQNDIALQLPELKKLLQVLREKR +SGCDDGKCGPGN
Sbjct: 61  YKNQADESSVASSCSIPSDSESIRQNDIALQLPELKKLLQVLREKRASSGCDDGKCGPGN 120

Query: 121 VFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHSR 180
           VFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVG DARLLYVGKTAG+HSR
Sbjct: 121 VFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGFHSR 180

Query: 181 TQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIS 240
           TQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGI+
Sbjct: 181 TQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIA 240

Query: 241 AELGIPLTHRGVATSVRFLTGHSRKGGTDPLYVAENAADPDSTLVVYMGLSTLPSLALKL 300
           AELGIPLTHRGVATSVRFLTGHSR+GGTDPL+VAENAADPDSTLVVYMGLSTLPSLALKL
Sbjct: 241 AELGIPLTHRGVATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKL 300

Query: 301 MHHGLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWS 360
           MHHGLPPDTPAAA+ERGTTPQQR VFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWS
Sbjct: 301 MHHGLPPDTPAAAIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWS 360

Query: 361 LSSKDASYLVEA 373
           LSSK+AS LVEA
Sbjct: 361 LSSKEASSLVEA 368

BLAST of Spg014118 vs. ExPASy Swiss-Prot
Match: Q42606 (S-adenosyl-L-methionine-dependent uroporphyrinogen III methyltransferase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=UPM1 PE=1 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 7.3e-150
Identity = 277/347 (79.83%), Postives = 301/347 (86.74%), Query Frame = 0

Query: 25  NFKPICSFHCSSSSSSSSSPFTEKHSVKRYQRDDWLYKNQADQSSIAPSCSIPCDSESIR 84
           N  PIC  H  +++SSSSSPFTEKHSV+RYQRD WLYK         PS S   D   +R
Sbjct: 23  NLTPICCLH-YNTASSSSSPFTEKHSVERYQRDQWLYKAVEPTPPSTPSPSPFEDEVFVR 82

Query: 85  QNDIALQLPELKKLLQVLREKRINSGCDDGKCGPGNVFLVGTGPGDPELLTLKAVKVIQS 144
           +NDIA QLPELKKLL VL+EKR+  GC  G CGPG+V+LVGTGPGDPELLTLKAV+VIQS
Sbjct: 83  ENDIASQLPELKKLLAVLKEKRV-KGCKGGDCGPGDVYLVGTGPGDPELLTLKAVRVIQS 142

Query: 145 ADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLKG 204
           ADLLLYDRLVSNDVL+LV PDARLLYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLKG
Sbjct: 143 ADLLLYDRLVSNDVLELVAPDARLLYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLKG 202

Query: 205 GDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGISAELGIPLTHRGVATSVRFLTGHSR 264
           GDPLVFGRGGEEMDFLQQQGI+V+++PGITAASGI+AELGIPLTHRGVATSVRFLTGHSR
Sbjct: 203 GDPLVFGRGGEEMDFLQQQGIRVQVIPGITAASGIAAELGIPLTHRGVATSVRFLTGHSR 262

Query: 265 KGGTDPLYVAENAADPDSTLVVYMGLSTLPSLALKLMHHGLPPDTPAAAVERGTTPQQRT 324
           KGGTDPL+VAENAADPD+TLVVYMGL TLPSLA KLM HGLP DTPA AVERGTTP QRT
Sbjct: 263 KGGTDPLFVAENAADPDTTLVVYMGLGTLPSLAQKLMDHGLPSDTPAVAVERGTTPLQRT 322

Query: 325 VFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSSKDASYLVE 372
           VFAELKD A EI++A LVSPTLIIIGKVV LSP W   +K++S LVE
Sbjct: 323 VFAELKDFATEIQSAGLVSPTLIIIGKVVELSPLWPHCTKESSCLVE 367

BLAST of Spg014118 vs. ExPASy Swiss-Prot
Match: A0KP37 (Siroheme synthase 2 OS=Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / BCRC 13018 / CCUG 14551 / JCM 1027 / KCTC 2358 / NCIMB 9240 / NCTC 8049) OX=380703 GN=cysG2 PE=3 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 6.8e-63
Identity = 132/252 (52.38%), Postives = 173/252 (68.65%), Query Frame = 0

Query: 104 EKRINSGCDDGKCGPGNVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVG 163
           E+ +N G D  K   G V LVG GPGDP LLTLKA++ IQ A+++LYD+LVS ++LDLV 
Sbjct: 199 EQWLNDGLDQAKNEVGEVVLVGAGPGDPGLLTLKALQQIQQAEVVLYDQLVSPEILDLVR 258

Query: 164 PDARLLYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQ 223
            DA L+ VGK AG HS  QEE + LL+ +A+AG  VVRLKGGDP +FGRGGEE++ L ++
Sbjct: 259 RDATLVSVGKKAGAHSVPQEETNRLLVEYAKAGNRVVRLKGGDPFMFGRGGEELEVLAEE 318

Query: 224 GIQVKIVPGITAASGISAELGIPLTHRGVATSVRFLTGHSRKGGTDPLYVAENAADPDST 283
           GI   +VPGITAA+G +A  GIPLTHR  A S  F+TGH +  G +P +  +  A    T
Sbjct: 319 GIPFSVVPGITAAAGATAYAGIPLTHRDHAQSAVFITGHCQIDGKEPDW--QQLAATSQT 378

Query: 284 LVVYMGLSTLPSLALKLMHHGLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVS 343
           LV+YMGL     +  +L+ HG    TP A +ERGTT +QR +   L DLA+   AA+ VS
Sbjct: 379 LVIYMGLMRSEHIQQQLVSHGRSSATPIAIIERGTTARQRVLTGTLADLAE--LAAQAVS 438

Query: 344 PTLIIIGKVVSL 356
           P+LI+IG+VV+L
Sbjct: 439 PSLIVIGEVVAL 446

BLAST of Spg014118 vs. ExPASy Swiss-Prot
Match: B8GUD3 (Siroheme synthase OS=Thioalkalivibrio sulfidiphilus (strain HL-EbGR7) OX=396588 GN=cysG PE=3 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 8.9e-63
Identity = 125/252 (49.60%), Postives = 170/252 (67.46%), Query Frame = 0

Query: 104 EKRINSGCDDGKCGPGNVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVG 163
           EK + +G D    G G VFLVG GPGDP+LLT +A++++Q AD+++YD LVS  +++LV 
Sbjct: 204 EKALETGLDTRDAG-GEVFLVGAGPGDPDLLTFRALRLMQLADVVVYDNLVSPAIIELVR 263

Query: 164 PDARLLYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQ 223
            DA ++Y GK    H+  QEEI++LL+  A+ G  V+RLKGGDP +FGRGGEE+D L Q+
Sbjct: 264 RDAEMIYAGKKRNLHTLPQEEINQLLVRLAKEGKRVLRLKGGDPFIFGRGGEEIDTLMQE 323

Query: 224 GIQVKIVPGITAASGISAELGIPLTHRGVATSVRFLTGHSRKGGTDPLYVAENAADPDST 283
           GI  ++VPGITAA+G ++  GIPLTHR  A +V F TGH R G  D  +  +  A P  T
Sbjct: 324 GIPFQVVPGITAAAGCASFSGIPLTHRDYAQAVVFATGHLRDGSIDLNW--KMLAQPRQT 383

Query: 284 LVVYMGLSTLPSLALKLMHHGLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVS 343
           +V YMGL  LP +  +LM HG+ PD P A VE+GTT  QR +   L  + D +K  ++  
Sbjct: 384 VVFYMGLLGLPIICRELMAHGVSPDMPMALVEQGTTQNQRVIVGTLASMPDLVKDYDVQP 443

Query: 344 PTLIIIGKVVSL 356
           PTLII+G+VV L
Sbjct: 444 PTLIIVGEVVKL 452

BLAST of Spg014118 vs. ExPASy Swiss-Prot
Match: Q6F8G6 (Siroheme synthase OS=Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1) OX=62977 GN=cysG PE=3 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 2.2e-61
Identity = 126/237 (53.16%), Postives = 162/237 (68.35%), Query Frame = 0

Query: 119 GNVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYH 178
           G V+LVG GPGDPELLTLKA++++Q AD+++YDRLVS  +L+L   DA  +YVGK    H
Sbjct: 216 GEVYLVGAGPGDPELLTLKALRLMQQADVVIYDRLVSAPILELCRRDAEKVYVGKARSNH 275

Query: 179 SRTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASG 238
           S  QE I+ LL+ +A+AG  V RLKGGDP +FGRGGEE+  L   GI  ++VPGITAASG
Sbjct: 276 SVPQEGINALLVKYAQAGKRVCRLKGGDPFIFGRGGEEIQELFAAGIPFQVVPGITAASG 335

Query: 239 ISAELGIPLTHRGVATSVRFLTGHSRKGGTDPLYVAENAADPDSTLVVYMGLSTLPSLAL 298
            SA  GIPLTHR  A SVRFLTGH ++G   P          + TLV+YMGL  L  +  
Sbjct: 336 CSAYAGIPLTHRDYAQSVRFLTGHLKEG--SPELPWSELVYENQTLVLYMGLVGLEHICQ 395

Query: 299 KLMHHGLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSL 356
           +L+ HG  PD P A V +GTTP+Q+ V   L ++A +I   ++ +PTL IIG+VVSL
Sbjct: 396 QLIAHGQRPDMPVALVSKGTTPEQKVVVGTLSNIASKIAEYQIHAPTLTIIGEVVSL 450

BLAST of Spg014118 vs. ExPASy Swiss-Prot
Match: Q0VQ05 (Siroheme synthase OS=Alcanivorax borkumensis (strain ATCC 700651 / DSM 11573 / NCIMB 13689 / SK2) OX=393595 GN=cysG PE=3 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 3.7e-61
Identity = 120/239 (50.21%), Postives = 162/239 (67.78%), Query Frame = 0

Query: 119 GNVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYH 178
           G V+LVG GPGDP+LLT +A++++Q AD++LYDRLV   ++DL   DA L+YVGK    H
Sbjct: 218 GEVYLVGAGPGDPDLLTFRALRLLQKADVVLYDRLVGKGIVDLARRDAELVYVGKARDKH 277

Query: 179 SRTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASG 238
           +  Q+ I+ELL+++A+ G  V RLKGGDP +FGRGGEE+D +  +GI  ++VPGITAASG
Sbjct: 278 ALPQDNINELLVHYAKQGKKVCRLKGGDPFIFGRGGEEIDLIVAEGIDFQVVPGITAASG 337

Query: 239 ISAELGIPLTHRGVATSVRFLTGHSRKGGTDPLYVAENAADPDSTLVVYMGLSTLPSLAL 298
            ++  GIPLTHR  A SVRF+TGH + G  D  +  ++      T+V YMGL  L  +  
Sbjct: 338 CASYAGIPLTHRDHAQSVRFVTGHRKDGSVDLDW--KHLVSETETVVFYMGLVGLREICS 397

Query: 299 KLMHHGLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSP 358
           +L+ HG   DTP A V RGTT  Q  +   L  L D+I+  E+ +PTLII+G VVSL P
Sbjct: 398 QLIAHGRGGDTPIALVSRGTTNLQEVITGRLDQLPDDIEGREIHAPTLIIVGSVVSLHP 454

BLAST of Spg014118 vs. ExPASy TrEMBL
Match: A0A6J1JU84 (uncharacterized protein LOC111488402 OS=Cucurbita maxima OX=3661 GN=LOC111488402 PE=3 SV=1)

HSP 1 Score: 680.6 bits (1755), Expect = 3.6e-192
Identity = 347/372 (93.28%), Postives = 359/372 (96.51%), Query Frame = 0

Query: 1   MARVYELQSLSSRFSSRPTVPRSPNFKPICSFHCSSSSSSSSSPFTEKHSVKRYQRDDWL 60
           MARV +LQSLSS FSSRPT+PRS NFKPICSFHC    SSSSSPFTEKHSVKRYQRDDW+
Sbjct: 1   MARVCDLQSLSSLFSSRPTIPRSSNFKPICSFHC----SSSSSPFTEKHSVKRYQRDDWV 60

Query: 61  YKNQADQSSIAPSCSIPCDSESIRQNDIALQLPELKKLLQVLREKRINSGCDDGKCGPGN 120
           YKNQAD+SS+A SCSI CDSESIRQNDIALQLPELKKLLQVLREKR +SGCDDGKCGPGN
Sbjct: 61  YKNQADESSVASSCSISCDSESIRQNDIALQLPELKKLLQVLREKRASSGCDDGKCGPGN 120

Query: 121 VFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHSR 180
           VFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVG DARLLYVGKTAG+HSR
Sbjct: 121 VFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGFHSR 180

Query: 181 TQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIS 240
           TQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGI+
Sbjct: 181 TQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIA 240

Query: 241 AELGIPLTHRGVATSVRFLTGHSRKGGTDPLYVAENAADPDSTLVVYMGLSTLPSLALKL 300
           AELGIPLTHRGVATSVRFLTGHSR+GGTDPL+VAENAADPDSTLVVYMGLSTLPSLALKL
Sbjct: 241 AELGIPLTHRGVATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKL 300

Query: 301 MHHGLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWS 360
           MHHGLPPDTPAAA+ERGTTPQQR VFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWS
Sbjct: 301 MHHGLPPDTPAAAIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWS 360

Query: 361 LSSKDASYLVEA 373
           LSSK+AS LVEA
Sbjct: 361 LSSKEASSLVEA 368

BLAST of Spg014118 vs. ExPASy TrEMBL
Match: A0A6J1GQM1 (uncharacterized protein LOC111456604 OS=Cucurbita moschata OX=3662 GN=LOC111456604 PE=3 SV=1)

HSP 1 Score: 680.6 bits (1755), Expect = 3.6e-192
Identity = 350/375 (93.33%), Postives = 362/375 (96.53%), Query Frame = 0

Query: 1   MARVYELQSLSSRFSSRPTVPRSPNFKPICSFHC---SSSSSSSSSPFTEKHSVKRYQRD 60
           MARV +LQSLSS F+SRPT+PRS NFKPICSFHC   SSSSSSSSSPFTEKHSVKRYQRD
Sbjct: 1   MARVCDLQSLSSLFASRPTIPRSSNFKPICSFHCSSSSSSSSSSSSPFTEKHSVKRYQRD 60

Query: 61  DWLYKNQADQSSIAPSCSIPCDSESIRQNDIALQLPELKKLLQVLREKRINSGCDDGKCG 120
           DWLYKNQAD+SS+A SCSIP DSESIRQNDIALQLPELKKLLQVLREKR +SG DDGKCG
Sbjct: 61  DWLYKNQADESSVASSCSIPSDSESIRQNDIALQLPELKKLLQVLREKRASSGGDDGKCG 120

Query: 121 PGNVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGY 180
           PGNVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVG DARLLYVGKTAG+
Sbjct: 121 PGNVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGF 180

Query: 181 HSRTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAAS 240
           HSRTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAAS
Sbjct: 181 HSRTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAAS 240

Query: 241 GISAELGIPLTHRGVATSVRFLTGHSRKGGTDPLYVAENAADPDSTLVVYMGLSTLPSLA 300
           GI+AELGIPLTHRGVATSVRFLTGHSR+GGTDPL+VAENAADPDSTLVVYMGLSTLPSLA
Sbjct: 241 GIAAELGIPLTHRGVATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSLA 300

Query: 301 LKLMHHGLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSP 360
           LKLMHHGLPPDTPAAA+ERGTTPQQR VFAELKDLADEIKAAELVSPTLIIIGKVVSLSP
Sbjct: 301 LKLMHHGLPPDTPAAAIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSLSP 360

Query: 361 HWSLSSKDASYLVEA 373
           HWSLSSK+AS LVEA
Sbjct: 361 HWSLSSKEASSLVEA 375

BLAST of Spg014118 vs. ExPASy TrEMBL
Match: A0A1S3CDT8 (uroporphyrinogen-III C-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103499894 PE=3 SV=1)

HSP 1 Score: 680.6 bits (1755), Expect = 3.6e-192
Identity = 343/372 (92.20%), Postives = 359/372 (96.51%), Query Frame = 0

Query: 1   MARVYELQSLSSRFSSRPTVPRSPNFKPICSFHCSSSSSSSSSPFTEKHSVKRYQRDDWL 60
           MAR  +LQSLSS FSS PT+PRSPNFKPI SFHCS SSSSSSSPFTEKHS+KRYQRDDWL
Sbjct: 1   MARFCDLQSLSSPFSSHPTIPRSPNFKPIFSFHCSYSSSSSSSPFTEKHSIKRYQRDDWL 60

Query: 61  YKNQADQSSIAPSCSIPCDSESIRQNDIALQLPELKKLLQVLREKRINSGCDDGKCGPGN 120
           YKNQ+DQ S+  SCSIP DSES+RQNDIA+QLPELKKLL+VLREKR++SGCDDGKCGPGN
Sbjct: 61  YKNQSDQPSVTSSCSIPYDSESMRQNDIAMQLPELKKLLEVLREKRVSSGCDDGKCGPGN 120

Query: 121 VFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHSR 180
           VFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVL+LVGPDARLLYVGKTAGYHSR
Sbjct: 121 VFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLELVGPDARLLYVGKTAGYHSR 180

Query: 181 TQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIS 240
           TQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGI+
Sbjct: 181 TQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIA 240

Query: 241 AELGIPLTHRGVATSVRFLTGHSRKGGTDPLYVAENAADPDSTLVVYMGLSTLPSLALKL 300
           AELGIPLTHRGVATSVRFLTGHSRKGGTDPL+VAENAADPDSTLVVYMGLSTLPSLALKL
Sbjct: 241 AELGIPLTHRGVATSVRFLTGHSRKGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKL 300

Query: 301 MHHGLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWS 360
           MHHGLPPDTPAAAVERGTTPQQRTVFA LKDLADEIKAAELVSPTLI+IG+VVSLSPHWS
Sbjct: 301 MHHGLPPDTPAAAVERGTTPQQRTVFAHLKDLADEIKAAELVSPTLIVIGRVVSLSPHWS 360

Query: 361 LSSKDASYLVEA 373
           LSS +AS LVEA
Sbjct: 361 LSSNEASSLVEA 372

BLAST of Spg014118 vs. ExPASy TrEMBL
Match: A0A0A0KBI8 (TP_methylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G428980 PE=3 SV=1)

HSP 1 Score: 679.5 bits (1752), Expect = 8.0e-192
Identity = 343/372 (92.20%), Postives = 360/372 (96.77%), Query Frame = 0

Query: 1   MARVYELQSLSSRFSSRPTVPRSPNFKPICSFHCSSSSSSSSSPFTEKHSVKRYQRDDWL 60
           MAR  +LQSLSS FSS PT+PRSPNFKPI SFHCSS+SSSSSSPFTEKHSVKRYQRDDWL
Sbjct: 1   MARFCDLQSLSSPFSSHPTIPRSPNFKPIFSFHCSSASSSSSSPFTEKHSVKRYQRDDWL 60

Query: 61  YKNQADQSSIAPSCSIPCDSESIRQNDIALQLPELKKLLQVLREKRINSGCDDGKCGPGN 120
           YK Q+DQ S+  SCSIP DSESIRQNDIA+QLPELKKLL+VLREKR+++GCDDGKCGPG+
Sbjct: 61  YKYQSDQPSVTSSCSIPYDSESIRQNDIAMQLPELKKLLEVLREKRVSNGCDDGKCGPGD 120

Query: 121 VFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHSR 180
           VFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVL+LVGPDARLLYVGKTAGYHSR
Sbjct: 121 VFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLELVGPDARLLYVGKTAGYHSR 180

Query: 181 TQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIS 240
           TQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGI+
Sbjct: 181 TQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIA 240

Query: 241 AELGIPLTHRGVATSVRFLTGHSRKGGTDPLYVAENAADPDSTLVVYMGLSTLPSLALKL 300
           AELGIPLTHRGVATSVRFLTGHSRKGGTDPLYVAENAADPDSTLVVYMGLSTLPSLALKL
Sbjct: 241 AELGIPLTHRGVATSVRFLTGHSRKGGTDPLYVAENAADPDSTLVVYMGLSTLPSLALKL 300

Query: 301 MHHGLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWS 360
           MHHGLPPDTPAAAVERGTTPQQRTVFA+LKDLADEIKAAELVSPTLI+IG+VVSLSPHWS
Sbjct: 301 MHHGLPPDTPAAAVERGTTPQQRTVFAQLKDLADEIKAAELVSPTLIVIGRVVSLSPHWS 360

Query: 361 LSSKDASYLVEA 373
           LSS +AS LVEA
Sbjct: 361 LSSNEASSLVEA 372

BLAST of Spg014118 vs. ExPASy TrEMBL
Match: A0A6J1D4K3 (uncharacterized protein LOC111017535 OS=Momordica charantia OX=3673 GN=LOC111017535 PE=3 SV=1)

HSP 1 Score: 664.8 bits (1714), Expect = 2.0e-187
Identity = 340/373 (91.15%), Postives = 356/373 (95.44%), Query Frame = 0

Query: 1   MARVYELQSLSSRFSSRPTVPRSPNFKPICSFHCSSSSSSSSSPFTEKHSVKRYQRDDWL 60
           MARVY+LQSLSS FSSRP  PR+P FKPI S HC    SSSSSPFTEKHS+KRYQRDDW+
Sbjct: 1   MARVYDLQSLSSPFSSRPRTPRTPIFKPISSIHC----SSSSSPFTEKHSIKRYQRDDWV 60

Query: 61  YKNQADQSSIAPSCSIPCDSESIRQNDIALQLPELKKLLQVLREKRINSGCDD-GKCGPG 120
           YKNQADQ+S A SCS+PCDS+SIRQ+DIALQLPELK+LL VLREKR+N GCDD G+CGPG
Sbjct: 61  YKNQADQASTALSCSVPCDSDSIRQDDIALQLPELKRLLMVLREKRVNGGCDDGGECGPG 120

Query: 121 NVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHS 180
           NVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHS
Sbjct: 121 NVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHS 180

Query: 181 RTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGI 240
           RTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQ+GIQVKIVPGITAASGI
Sbjct: 181 RTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQKGIQVKIVPGITAASGI 240

Query: 241 SAELGIPLTHRGVATSVRFLTGHSRKGGTDPLYVAENAADPDSTLVVYMGLSTLPSLALK 300
           +AELGIPLTHRGVAT+VRFLTGHSRKGGTDPL+VAENAAD DSTLVVYMGLSTLPSLALK
Sbjct: 241 AAELGIPLTHRGVATNVRFLTGHSRKGGTDPLFVAENAADLDSTLVVYMGLSTLPSLALK 300

Query: 301 LMHHGLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHW 360
           LMHHGLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHW
Sbjct: 301 LMHHGLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHW 360

Query: 361 SLSSKDASYLVEA 373
           SLSSK+AS LVEA
Sbjct: 361 SLSSKEASCLVEA 369

BLAST of Spg014118 vs. TAIR 10
Match: AT5G40850.1 (urophorphyrin methylase 1 )

HSP 1 Score: 531.6 bits (1368), Expect = 5.2e-151
Identity = 277/347 (79.83%), Postives = 301/347 (86.74%), Query Frame = 0

Query: 25  NFKPICSFHCSSSSSSSSSPFTEKHSVKRYQRDDWLYKNQADQSSIAPSCSIPCDSESIR 84
           N  PIC  H  +++SSSSSPFTEKHSV+RYQRD WLYK         PS S   D   +R
Sbjct: 23  NLTPICCLH-YNTASSSSSPFTEKHSVERYQRDQWLYKAVEPTPPSTPSPSPFEDEVFVR 82

Query: 85  QNDIALQLPELKKLLQVLREKRINSGCDDGKCGPGNVFLVGTGPGDPELLTLKAVKVIQS 144
           +NDIA QLPELKKLL VL+EKR+  GC  G CGPG+V+LVGTGPGDPELLTLKAV+VIQS
Sbjct: 83  ENDIASQLPELKKLLAVLKEKRV-KGCKGGDCGPGDVYLVGTGPGDPELLTLKAVRVIQS 142

Query: 145 ADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLKG 204
           ADLLLYDRLVSNDVL+LV PDARLLYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLKG
Sbjct: 143 ADLLLYDRLVSNDVLELVAPDARLLYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLKG 202

Query: 205 GDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGISAELGIPLTHRGVATSVRFLTGHSR 264
           GDPLVFGRGGEEMDFLQQQGI+V+++PGITAASGI+AELGIPLTHRGVATSVRFLTGHSR
Sbjct: 203 GDPLVFGRGGEEMDFLQQQGIRVQVIPGITAASGIAAELGIPLTHRGVATSVRFLTGHSR 262

Query: 265 KGGTDPLYVAENAADPDSTLVVYMGLSTLPSLALKLMHHGLPPDTPAAAVERGTTPQQRT 324
           KGGTDPL+VAENAADPD+TLVVYMGL TLPSLA KLM HGLP DTPA AVERGTTP QRT
Sbjct: 263 KGGTDPLFVAENAADPDTTLVVYMGLGTLPSLAQKLMDHGLPSDTPAVAVERGTTPLQRT 322

Query: 325 VFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSSKDASYLVE 372
           VFAELKD A EI++A LVSPTLIIIGKVV LSP W   +K++S LVE
Sbjct: 323 VFAELKDFATEIQSAGLVSPTLIIIGKVVELSPLWPHCTKESSCLVE 367

BLAST of Spg014118 vs. TAIR 10
Match: AT5G40850.2 (urophorphyrin methylase 1 )

HSP 1 Score: 477.2 bits (1227), Expect = 1.2e-134
Identity = 248/317 (78.23%), Postives = 269/317 (84.86%), Query Frame = 0

Query: 25  NFKPICSFHCSSSSSSSSSPFTEKHSVKRYQRDDWLYKNQADQSSIAPSCSIPCDSESIR 84
           N  PIC  H  +++SSSSSPFTEKHSV+RYQRD WLYK         PS S   D   +R
Sbjct: 23  NLTPICCLH-YNTASSSSSPFTEKHSVERYQRDQWLYKAVEPTPPSTPSPSPFEDEVFVR 82

Query: 85  QNDIALQLPELKKLLQVLREKRINSGCDDGKCGPGNVFLVGTGPGDPELLTLKAVKVIQS 144
           +NDIA QLPELKKLL VL+EKR+  GC  G CGPG+V+LVGTGPGDPELLTLKAV+VIQS
Sbjct: 83  ENDIASQLPELKKLLAVLKEKRV-KGCKGGDCGPGDVYLVGTGPGDPELLTLKAVRVIQS 142

Query: 145 ADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLKG 204
           ADLLLYDRLVSNDVL+LV PDARLLYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLKG
Sbjct: 143 ADLLLYDRLVSNDVLELVAPDARLLYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLKG 202

Query: 205 GDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGISAELGIPLTHRGVATSVRFLTGHSR 264
           GDPLVFGRGGEEMDFLQQQGI+V+++PGITAASGI+AELGIPLTHRGVATSVRFLTGHSR
Sbjct: 203 GDPLVFGRGGEEMDFLQQQGIRVQVIPGITAASGIAAELGIPLTHRGVATSVRFLTGHSR 262

Query: 265 KGGTDPLYVAENAADPDSTLVVYMGLSTLPSLALKLMHHGLPPDTPAAAVERGTTPQQRT 324
           KGGTDPL+VAENAADPD+TLVVYMGL TLPSLA KLM HGLP DTPA AVERGTTP QRT
Sbjct: 263 KGGTDPLFVAENAADPDTTLVVYMGLGTLPSLAQKLMDHGLPSDTPAVAVERGTTPLQRT 322

Query: 325 VFAELKDLADEIKAAEL 342
            F  L      +K  +L
Sbjct: 323 AFGILCRFLLSLKILQL 337

BLAST of Spg014118 vs. TAIR 10
Match: AT1G45110.1 (Tetrapyrrole (Corrin/Porphyrin) Methylases )

HSP 1 Score: 49.3 bits (116), Expect = 7.8e-06
Identity = 46/156 (29.49%), Postives = 71/156 (45.51%), Query Frame = 0

Query: 112 DDGKCGP--GNVFLVGTGPGDPELLTLKAVKVIQSADLLL-YDRLVSNDVLDLVGPDARL 171
           DD K GP    ++LVGT  G+ E +TL+A++V++SAD++L  D   S  +L      A+L
Sbjct: 46  DDSKRGPLKPGLYLVGTPIGNLEDITLRAIRVLRSADVILSEDTRHSGKLLQYYNIKAQL 105

Query: 172 LYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLK-GGDPLVFGRGGEEMDFLQQQGIQV 231
           L       YH   + +  + +L   + G  V  +   G P +   G +      ++ I V
Sbjct: 106 L------SYHKFNEAQREQAVLTRLKQGEIVALISDAGTPGISDPGTQLAKMCAKENIDV 165

Query: 232 KIVPGITAASGISAELGIPLTHRGVATSVRFLTGHS 264
             +PG   A  + A L          T V FL  HS
Sbjct: 166 IPIPG---ACAVVAALSASGLETDEFTFVGFLPKHS 192

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6575668.17.5e-19294.09hypothetical protein SDJN03_26307, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022954326.17.5e-19293.33uncharacterized protein LOC111456604 [Cucurbita moschata][more]
XP_022991905.17.5e-19293.28uncharacterized protein LOC111488402 [Cucurbita maxima][more]
XP_008461254.17.5e-19292.20PREDICTED: uroporphyrinogen-III C-methyltransferase [Cucumis melo][more]
XP_023548332.11.3e-19193.28uncharacterized protein LOC111807000 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q426067.3e-15079.83S-adenosyl-L-methionine-dependent uroporphyrinogen III methyltransferase, chloro... [more]
A0KP376.8e-6352.38Siroheme synthase 2 OS=Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 ... [more]
B8GUD38.9e-6349.60Siroheme synthase OS=Thioalkalivibrio sulfidiphilus (strain HL-EbGR7) OX=396588 ... [more]
Q6F8G62.2e-6153.16Siroheme synthase OS=Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1) OX=... [more]
Q0VQ053.7e-6150.21Siroheme synthase OS=Alcanivorax borkumensis (strain ATCC 700651 / DSM 11573 / N... [more]
Match NameE-valueIdentityDescription
A0A6J1JU843.6e-19293.28uncharacterized protein LOC111488402 OS=Cucurbita maxima OX=3661 GN=LOC111488402... [more]
A0A6J1GQM13.6e-19293.33uncharacterized protein LOC111456604 OS=Cucurbita moschata OX=3662 GN=LOC1114566... [more]
A0A1S3CDT83.6e-19292.20uroporphyrinogen-III C-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103499894... [more]
A0A0A0KBI88.0e-19292.20TP_methylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G42898... [more]
A0A6J1D4K32.0e-18791.15uncharacterized protein LOC111017535 OS=Momordica charantia OX=3673 GN=LOC111017... [more]
Match NameE-valueIdentityDescription
AT5G40850.15.2e-15179.83urophorphyrin methylase 1 [more]
AT5G40850.21.2e-13478.23urophorphyrin methylase 1 [more]
AT1G45110.17.8e-0629.49Tetrapyrrole (Corrin/Porphyrin) Methylases [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000878Tetrapyrrole methylasePFAMPF00590TP_methylasecoord: 121..333
e-value: 7.1E-50
score: 169.9
IPR014777Tetrapyrrole methylase, subdomain 1GENE3D3.40.1010.10coord: 112..234
e-value: 9.9E-42
score: 144.1
IPR014776Tetrapyrrole methylase, subdomain 2GENE3D3.30.950.10coord: 235..371
e-value: 1.8E-44
score: 153.0
IPR006366Uroporphyrin-III C-methyltransferaseTIGRFAMTIGR01469TIGR01469coord: 119..355
e-value: 8.1E-87
score: 288.9
IPR006366Uroporphyrin-III C-methyltransferaseCDDcd11642SUMTcoord: 124..353
e-value: 1.18014E-126
score: 361.369
NoneNo IPR availablePANTHERPTHR45790:SF3UROPORPHYRINOGEN-III C-METHYLTRANSFERASEcoord: 115..371
NoneNo IPR availablePANTHERPTHR45790SIROHEME SYNTHASE-RELATEDcoord: 115..371
IPR003043Uroporphiryn-III C-methyltransferase, conserved sitePROSITEPS00840SUMT_2coord: 199..232
IPR003043Uroporphiryn-III C-methyltransferase, conserved sitePROSITEPS00839SUMT_1coord: 124..138
IPR035996Tetrapyrrole methylase superfamilySUPERFAMILY53790Tetrapyrrole methylasecoord: 118..359

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg014118.1Spg014118.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
biological_process GO:0019354 siroheme biosynthetic process
molecular_function GO:0004851 uroporphyrin-III C-methyltransferase activity
molecular_function GO:0008168 methyltransferase activity