Spg013975 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg013975
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Description1,3-beta-glucan synthase
Locationscaffold3: 46639335 .. 46643829 (-)
RNA-Seq ExpressionSpg013975
SyntenySpg013975
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTAAACAGCATTGTAAAGCAGAATATTAAAAGAGAATTGGGCAAAGGAAATCACATCTCTTTTTGGCATGATGTCTGGATCCAAAATCACACTTTGGCTTCTTTATATCCAAGACTGTACAGACTTTCCACAAGTACTCATGCTTCGGTGGCCAATGTATGGAAAGCTGATAATGGAGTTTGGGACATGGGTTTCAGGAGAAACCTAAATGATTCTGAAATAGTTGAATGGGCCTCCTTGTCTCATTGCCTTTCATCTGTTGTTTTAAAAAATAACAATGACTCGTGGAGTTGGAATTATAGATCATCTAAAACTTTTTCAGTCCATTCGATGATGGAGGTTTTAACACTACGGGGAGGTTATTCTATAGAACCTTTATATTCAGTTATATGGAAAGGATCTTATCCCCAGAAAATTAAGATTTTCCTATGGGAGCTTAGCCATAGTGGTTTTAACACCATGGATCGCCTTCAACAACGACAACCTTATCTCTCCCTTTCACCTTCATGTTGTATTATGTGTTTTGCAAGTTCAGAATCTCCATGTCATCTCTTCGGTTATTGTTCCTTTGCTCAACAATATTGGAATTTTATTCCGACGGCCTTTGGATGGAATACAGTTCTACCTGGAAATATTTTCGACCTCTTATCTATGGTTTTTGTGGGTCACCCTCTCAAAGGGGATGCGAAAACCCTTTGGATGGCTATTAATCGATCCTTTTTCTGGACTCTTTGGAATGAACGGAACGGGAGGATCTTTAATGACACCATTGGATCTTTTGATGGTTTTCTAAATTTAGTTCTTTTTAATGCTTTATATTGGTGTAAATGTTCACCTCCTTTTAAAGATTACAGTCTCTCTTCTCTTATTAACCACTGGACATCCTTTTTGTAAGCCACTATTAGTGGTATCATTTCATTTATCAATGAAATAGTTATGTTTCTTTTCAAAAAAAAGATGGAGTCCCAATAAAAACTTTAACACTTTTCCATTGGAAAATGACTTCTAACCCAAGCCAATTAGGACCAAAATAACCACACCAACACGTCAGAGAGGATTCCAGCCACACATGCCTTCACGCACCGACACGTGGGAATGGCGACAAATTGGAGTGGCGGTAGAAGAGCCTCACATTCCGGTGAGAAGCGGACTGATTTTAGGGTTTGGCTGATCGATGACTAGGCTTCCTCTTGGAATGGGTGGTAACAGAAAAAGACGGAAAAAAAAAAAGACCAAACTTGCAACAACAGACAGTGGCGACGGCAACAGAGGCAGATACTAATAGCGGCAACGGGAATAGGTGGGAACAACAATTCCACAAAACCCAAGGTTGAAAACAAAAGGGTCCTAAATCTCAAACACAAAACACTCATGGTTAACAAAACCCTAATTGCCCTAGAAAAAACCTAGAGCTATGATTCCATGTTATTGCCATTCCAAATATGAGAGAATCATTCACCTTCTTTTTAATAGCGTTAAAAAAATATAAGCATACATGAAAGCCCTAAGCTAATAATAGTAAAATTACAATAAAGTACAGAAAACTAATAAGCTAATATATTTACATATATATACTATAACACTATTATTCCTCTATAAACATGGGTTTAAGGGATTTGTATTAGGAGAACTTGATATTCTTTGGAGATCGAATAAAGACTTTGTTTAATAGTTCTTTGAGAGATCTTCGGCTCCATCATAAGCAGAACAACAAGAAGGATTCATTATTGTACCATTTGTGATGATTAGAATGTACTATTAAGGTCTGAACTTCATTTTGTTAATTTTCTTTTGTTATAAATTATATTAGTGACCATCACATATTTTGGGTGTCTTAATGCTCAATCTTACTCACCTAAATTGAATGTATCATATACCTTTGGCTTCTTATTCAGAGTTCGATTCCATTATGGCCATCCAGATGTTTTTGATAGACTGTTTCACCTTACCAGAGGGGGTGTTAGTAAAGCTTCCAAAGTCATCAACCTGAGTGAGGACATATTTGCAGGCATGTTCATTGTCAAAAAAAAGCCCTTCGTTTTATATTCAAACGAGTAATCTGGTTTAATCATATATTCCATTCCTATGGATGTGATTTTGAATTGCAGGTTTCAACTCCACCTTGCGTGAAGGTAATGTTACTCATCATGAATACATACAAGTTGGTAAAGGGAGAGATGTTGGCCTTAACCAGATCTCCATGTTTGAGGCTAAGATAGCCAATGGTAATGGTGAACAGACATTAAGCCGTGACATATACAGGCTAGGTCACCGGTTTGATTTTTTTCGCATGCTATCTTGTTACTTCACCACCATTGGTTTCTATTTCAGTACTATGGTACGTATGAGAAGTCTGTTTTTATTGCATCTTTATCCACATTTCTTTGCTCATGAGACGGGCTTGATTCTTCATTGACAGCTGACTGTGCTCACAGTCTATGTCTTTCTATATGGCCGCCTTTACCTCGTTCTCAGTGGCCTTGAAAAACAATTGAGTACTCATCCTGCCATTAAAGACAACAAGTCCCTTCAAACAGCTCTTATTTCTCAGTCCGCTGTGCAAATTGGACTTCTGATGGCATTGCCTATGATGGTTGAAATTGGACTGGAAAGGGGCTTTCGGGCAGCTTTAAGTGATTTCATACTAATGCAGCTTCAGCTTGCCCCTGTGTTTTTCACATTCTCTTTGGGAACTAAGACGCATTATTATGGAAGGACATTGCTTCACGGGGGTGCACAGTACAGAGGTACAGGTCGTGGCTTTGTGGTGTTCCATGCAAAATTTGCAGACAATTACAGGATGTATTCTCGCAGCCATTTTGTCAAAGGAATTGAATTGATGATTCTTCTTATCGTTTACCACATACTTGGAGTTTCATATAGAGGTGTGGTTGCTCATGTATTGATCACCATATCAATATGGTTTATGGTAGGAACGTGGCTTTTTGCTCCTTTCCTCTTCAATCCATCAGGCTTTGAGTGGCAAAAGATAGTTGATGACTGGACAGATTGGAAAAAATGGATAGTCAACCGTGGAGGAATCGGGGTATCTCCTGATAAAAGTTGGGAGTCTTGGTGGGAAAAGGAACATGAACATCTACTTTACTCTGGAAAGCGTGGCATCTTTGTTGAGATATTACTGTCACTACGATTTTTCATTTTTCAGTATGGACTAGTATATCACTTGAAAATAATCGACTCCCAAAGTTTTCTGGTACGAATCTTTTCCCACATTCTTACGAATTCCTTGGTTTTATTTTTACCAAGCAGATAAGCATATTTATTCTTCTTAATTATCACTTAAAACTTCAGGTTTATGGCCTTTCCTGGGTTGTGATCATTTCAATATTGCTCCTGATGAAGGTAAGAAAAAATTTCTTCTGGATCCTCCTCCTTTACTTAGGTTAAACCACAAACTTGTGTTTAGGTATCTTATTTGGTTAGATCTTGTTAACTTATCAATAAATAGTTGGGCCTCCAAGTAGGGAGACTTTTTATAAACTAGGGCCATTTATATTATTATAGAAAAGAGAAACGTAGTCCTATATAGGGCAGGGATCCATAACGACACTCATATTTGCCTTAAGAAAGCCATAGATTGGGACTTCTCCATGTTGCGCTTGGGGATTTTTTTTTCCCAGGAGTTGAATTCCTGTAGATGGAGACTTTCTTCAAAAAAGGAGATTGATGGGACAAAATAGTTTGCTATTTTCCATTTTATAACCAGAAGTTAGAAATGTGTATTTTTTGTATGATTATTTTGTTTCTAAATAATCGAAAGCAGATCTAAAGGCCCAGAGGATATACTACTCAATGTAACTTTATAAACACTAAGAACTTTCATTACCAGTTCTTCATCAGCTCTGCTTATTTAAAATGAATAGTATGGAGCCACTACTTTCCTGTTGCTGCTGGGATAACCTTAACACCATTCAAATTGTGTAGGCTGTATCCGTTGGACGACGTAAATTCAGCGCTAGCTTTCAGTTGTTATTTCGGCTGGCCGAGGGTTTTATTTTCATTATCTGTGTAGCTGGTTTCATTACTTTAGTTGCAGTGCCTCATATGACAATCAGGGACATCATACTTTGCCTTCTTGCCTTCTTGCCAACAGGATGGGGCCTGCTCTTGGTCAGTTTTCTGAAACATCTCCATGCTAGATACTTTGGATGTTAGTATTACAATCCTTTTTGCTCACTAATTTATCAATTGTGTTCATCTTCATGGTTCAATACAGATTGCACAAGCTTGTAAGCGCTTAATTCATCATACTGCATTATGGGGATCTGTTCGTGCCCTGGCTCGGAGTTATGAAATTCTCATGGGCTTGCTTCTCTTCACACCTGTAGCATTCCTGGCCTGGTTTCCATTCGTTTCGGAGTTCCAGACACGAATGCTGTTCAACCAAGCATTCAGCAGAGGTTTGCAAATTTCTCGTATTCTTGGCGGGCCTAGGAAGAAGGAAAAGAGTTCCTCCACAGACAAAGAGTAA

mRNA sequence

ATGTTAAACAGCATTGTAAAGCAGAATATTAAAAGAGAATTGGGCAAAGGAAATCACATCTCTTTTTGGCATGATGTCTGGATCCAAAATCACACTTTGGCTTCTTTATATCCAAGACTGTACAGACTTTCCACAAGTACTCATGCTTCGGTGGCCAATGTATGGAAAGCTGATAATGGAGTTTGGGACATGGGTTTCAGGAGAAACCTAAATGATTCTGAAATAGTTGAATGGGCCTCCTTGTCTCATTGCCTTTCATCTGTTGTTTTAAAAAATAACAATGACTCGTGGAGTTGGAATTATAGATCATCTAAAACTTTTTCAGTCCATTCGATGATGGAGGTTTTAACACTACGGGGAGGTTATTCTATAGAACCTTTATATTCAGTTATATGGAAAGGATCTTATCCCCAGAAAATTAAGATTTTCCTATGGGAGCTTAGCCATAGTGGTTTTAACACCATGGATCGCCTTCAACAACGACAACCTTATCTCTCCCTTTCACCTTCATGTTGTATTATGTGTTTTGCAAGTTCAGAATCTCCATGTCATCTCTTCGGTTATTGTTCCTTTGCTCAACAATATTGGAATTTTATTCCGACGGCCTTTGGATGGAATACAGTTCTACCTGGAAATATTTTCGACCTCTTATCTATGGTTTTTGTGGGTCACCCTCTCAAAGGGGATGCGAAAACCCTTTGGATGGCTATTAATCGATCCTTTTTCTGGACTCTTTGGAATGAACGGAACGGGAGGATCTTTAATGACACCATTGGATCTTTTGATGGTTTCAACTCCACCTTGCGTGAAGGTAATGTTACTCATCATGAATACATACAAGTTGGTAAAGGGAGAGATGTTGGCCTTAACCAGATCTCCATGTTTGAGGCTAAGATAGCCAATGGTAATGGTGAACAGACATTAAGCCGTGACATATACAGGCTAGGTCACCGGTTTGATTTTTTTCGCATGCTATCTTGTTACTTCACCACCATTGGTTTCTATTTCAGTACTATGCTGACTGTGCTCACAGTCTATGTCTTTCTATATGGCCGCCTTTACCTCGTTCTCAGTGGCCTTGAAAAACAATTGAGTACTCATCCTGCCATTAAAGACAACAAGTCCCTTCAAACAGCTCTTATTTCTCAGTCCGCTGTGCAAATTGGACTTCTGATGGCATTGCCTATGATGGTTGAAATTGGACTGGAAAGGGGCTTTCGGGCAGCTTTAAGTGATTTCATACTAATGCAGCTTCAGCTTGCCCCTGTGTTTTTCACATTCTCTTTGGGAACTAAGACGCATTATTATGGAAGGACATTGCTTCACGGGGGTGCACAGTACAGAGGTACAGGTCGTGGCTTTGTGGTGTTCCATGCAAAATTTGCAGACAATTACAGGATGTATTCTCGCAGCCATTTTGTCAAAGGAATTGAATTGATGATTCTTCTTATCGTTTACCACATACTTGGAGTTTCATATAGAGGTGTGGTTGCTCATGTATTGATCACCATATCAATATGGTTTATGGTAGGAACGTGGCTTTTTGCTCCTTTCCTCTTCAATCCATCAGGCTTTGAGTGGCAAAAGATAGTTGATGACTGGACAGATTGGAAAAAATGGATAGTCAACCGTGGAGGAATCGGGGTATCTCCTGATAAAAGTTGGGAGTCTTGGTGGGAAAAGGAACATGAACATCTACTTTACTCTGGAAAGCGTGGCATCTTTGTTGAGATATTACTGTCACTACGATTTTTCATTTTTCAGTATGGACTAGTATATCACTTGAAAATAATCGACTCCCAAAGTTTTCTGGTTTATGGCCTTTCCTGGGTTGTGATCATTTCAATATTGCTCCTGATGAAGGCTGTATCCGTTGGACGACGTAAATTCAGCGCTAGCTTTCAGTTGTTATTTCGGCTGGCCGAGGGTTTTATTTTCATTATCTGTGTAGCTGGTTTCATTACTTTAGTTGCAGTGCCTCATATGACAATCAGGGACATCATACTTTGCCTTCTTGCCTTCTTGCCAACAGGATGGGGCCTGCTCTTGATTGCACAAGCTTGTAAGCGCTTAATTCATCATACTGCATTATGGGGATCTGTTCGTGCCCTGGCTCGGAGTTATGAAATTCTCATGGGCTTGCTTCTCTTCACACCTGTAGCATTCCTGGCCTGGTTTCCATTCGTTTCGGAGTTCCAGACACGAATGCTGTTCAACCAAGCATTCAGCAGAGGTTTGCAAATTTCTCGTATTCTTGGCGGGCCTAGGAAGAAGGAAAAGAGTTCCTCCACAGACAAAGAGTAA

Coding sequence (CDS)

ATGTTAAACAGCATTGTAAAGCAGAATATTAAAAGAGAATTGGGCAAAGGAAATCACATCTCTTTTTGGCATGATGTCTGGATCCAAAATCACACTTTGGCTTCTTTATATCCAAGACTGTACAGACTTTCCACAAGTACTCATGCTTCGGTGGCCAATGTATGGAAAGCTGATAATGGAGTTTGGGACATGGGTTTCAGGAGAAACCTAAATGATTCTGAAATAGTTGAATGGGCCTCCTTGTCTCATTGCCTTTCATCTGTTGTTTTAAAAAATAACAATGACTCGTGGAGTTGGAATTATAGATCATCTAAAACTTTTTCAGTCCATTCGATGATGGAGGTTTTAACACTACGGGGAGGTTATTCTATAGAACCTTTATATTCAGTTATATGGAAAGGATCTTATCCCCAGAAAATTAAGATTTTCCTATGGGAGCTTAGCCATAGTGGTTTTAACACCATGGATCGCCTTCAACAACGACAACCTTATCTCTCCCTTTCACCTTCATGTTGTATTATGTGTTTTGCAAGTTCAGAATCTCCATGTCATCTCTTCGGTTATTGTTCCTTTGCTCAACAATATTGGAATTTTATTCCGACGGCCTTTGGATGGAATACAGTTCTACCTGGAAATATTTTCGACCTCTTATCTATGGTTTTTGTGGGTCACCCTCTCAAAGGGGATGCGAAAACCCTTTGGATGGCTATTAATCGATCCTTTTTCTGGACTCTTTGGAATGAACGGAACGGGAGGATCTTTAATGACACCATTGGATCTTTTGATGGTTTCAACTCCACCTTGCGTGAAGGTAATGTTACTCATCATGAATACATACAAGTTGGTAAAGGGAGAGATGTTGGCCTTAACCAGATCTCCATGTTTGAGGCTAAGATAGCCAATGGTAATGGTGAACAGACATTAAGCCGTGACATATACAGGCTAGGTCACCGGTTTGATTTTTTTCGCATGCTATCTTGTTACTTCACCACCATTGGTTTCTATTTCAGTACTATGCTGACTGTGCTCACAGTCTATGTCTTTCTATATGGCCGCCTTTACCTCGTTCTCAGTGGCCTTGAAAAACAATTGAGTACTCATCCTGCCATTAAAGACAACAAGTCCCTTCAAACAGCTCTTATTTCTCAGTCCGCTGTGCAAATTGGACTTCTGATGGCATTGCCTATGATGGTTGAAATTGGACTGGAAAGGGGCTTTCGGGCAGCTTTAAGTGATTTCATACTAATGCAGCTTCAGCTTGCCCCTGTGTTTTTCACATTCTCTTTGGGAACTAAGACGCATTATTATGGAAGGACATTGCTTCACGGGGGTGCACAGTACAGAGGTACAGGTCGTGGCTTTGTGGTGTTCCATGCAAAATTTGCAGACAATTACAGGATGTATTCTCGCAGCCATTTTGTCAAAGGAATTGAATTGATGATTCTTCTTATCGTTTACCACATACTTGGAGTTTCATATAGAGGTGTGGTTGCTCATGTATTGATCACCATATCAATATGGTTTATGGTAGGAACGTGGCTTTTTGCTCCTTTCCTCTTCAATCCATCAGGCTTTGAGTGGCAAAAGATAGTTGATGACTGGACAGATTGGAAAAAATGGATAGTCAACCGTGGAGGAATCGGGGTATCTCCTGATAAAAGTTGGGAGTCTTGGTGGGAAAAGGAACATGAACATCTACTTTACTCTGGAAAGCGTGGCATCTTTGTTGAGATATTACTGTCACTACGATTTTTCATTTTTCAGTATGGACTAGTATATCACTTGAAAATAATCGACTCCCAAAGTTTTCTGGTTTATGGCCTTTCCTGGGTTGTGATCATTTCAATATTGCTCCTGATGAAGGCTGTATCCGTTGGACGACGTAAATTCAGCGCTAGCTTTCAGTTGTTATTTCGGCTGGCCGAGGGTTTTATTTTCATTATCTGTGTAGCTGGTTTCATTACTTTAGTTGCAGTGCCTCATATGACAATCAGGGACATCATACTTTGCCTTCTTGCCTTCTTGCCAACAGGATGGGGCCTGCTCTTGATTGCACAAGCTTGTAAGCGCTTAATTCATCATACTGCATTATGGGGATCTGTTCGTGCCCTGGCTCGGAGTTATGAAATTCTCATGGGCTTGCTTCTCTTCACACCTGTAGCATTCCTGGCCTGGTTTCCATTCGTTTCGGAGTTCCAGACACGAATGCTGTTCAACCAAGCATTCAGCAGAGGTTTGCAAATTTCTCGTATTCTTGGCGGGCCTAGGAAGAAGGAAAAGAGTTCCTCCACAGACAAAGAGTAA

Protein sequence

MLNSIVKQNIKRELGKGNHISFWHDVWIQNHTLASLYPRLYRLSTSTHASVANVWKADNGVWDMGFRRNLNDSEIVEWASLSHCLSSVVLKNNNDSWSWNYRSSKTFSVHSMMEVLTLRGGYSIEPLYSVIWKGSYPQKIKIFLWELSHSGFNTMDRLQQRQPYLSLSPSCCIMCFASSESPCHLFGYCSFAQQYWNFIPTAFGWNTVLPGNIFDLLSMVFVGHPLKGDAKTLWMAINRSFFWTLWNERNGRIFNDTIGSFDGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRDIYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTVYVFLYGRLYLVLSGLEKQLSTHPAIKDNKSLQTALISQSAVQIGLLMALPMMVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAQYRGTGRGFVVFHAKFADNYRMYSRSHFVKGIELMILLIVYHILGVSYRGVVAHVLITISIWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVSPDKSWESWWEKEHEHLLYSGKRGIFVEILLSLRFFIFQYGLVYHLKIIDSQSFLVYGLSWVVIISILLLMKAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAVPHMTIRDIILCLLAFLPTGWGLLLIAQACKRLIHHTALWGSVRALARSYEILMGLLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGPRKKEKSSSTDKE
Homology
BLAST of Spg013975 vs. NCBI nr
Match: XP_038898164.1 (callose synthase 1-like isoform X2 [Benincasa hispida])

HSP 1 Score: 972.6 bits (2513), Expect = 2.0e-279
Identity = 495/516 (95.93%), Postives = 506/516 (98.06%), Query Frame = 0

Query: 252  RIFNDTIGSFDGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD 311
            ++ N +   F GFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD
Sbjct: 1140 KVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD 1199

Query: 312  IYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTVYVFLYGRLYLVLSGLEKQLSTHPAIK 371
            +YRLGHRFDFFRMLSCYFTT+GFYFST+LTVLTVYVFLYGRLYLVLSGLEK LSTHPAIK
Sbjct: 1200 MYRLGHRFDFFRMLSCYFTTVGFYFSTLLTVLTVYVFLYGRLYLVLSGLEKALSTHPAIK 1259

Query: 372  DNKSLQTALISQSAVQIGLLMALPMMVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGT 431
            DNKSLQTALISQSAVQIGLLMALPMMVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGT
Sbjct: 1260 DNKSLQTALISQSAVQIGLLMALPMMVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGT 1319

Query: 432  KTHYYGRTLLHGGAQYRGTGRGFVVFHAKFADNYRMYSRSHFVKGIELMILLIVYHILGV 491
            KTHYYGRTLLHGGA+YRGTGRGFVVFHAKFADNYRMYSRSHFVKGIELMILL+VYHILGV
Sbjct: 1320 KTHYYGRTLLHGGAEYRGTGRGFVVFHAKFADNYRMYSRSHFVKGIELMILLLVYHILGV 1379

Query: 492  SYRGVVAHVLITISIWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVSP 551
            SYRGVVAHVLITISIWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVSP
Sbjct: 1380 SYRGVVAHVLITISIWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVSP 1439

Query: 552  DKSWESWWEKEHEHLLYSGKRGIFVEILLSLRFFIFQYGLVYHLKIIDSQSFLVYGLSWV 611
            DKSWESWWEKEH+HL YSGKRGIF EILLSLRFFIFQYGLVYHLKIIDSQSFLVYGLSWV
Sbjct: 1440 DKSWESWWEKEHDHLKYSGKRGIFAEILLSLRFFIFQYGLVYHLKIIDSQSFLVYGLSWV 1499

Query: 612  VIISILLLMKAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAVPHMTIRDIILCL 671
            VIISILLLMKAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVA+PHMTIRDIILCL
Sbjct: 1500 VIISILLLMKAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAIPHMTIRDIILCL 1559

Query: 672  LAFLPTGWGLLLIAQACKRLIHHTALWGSVRALARSYEILMGLLLFTPVAFLAWFPFVSE 731
            LAFLPTGWGLLLIAQACK LIHHTALWGSVRALARSYEI+MGLLLFTPVAFLAWFPFVSE
Sbjct: 1560 LAFLPTGWGLLLIAQACKPLIHHTALWGSVRALARSYEIVMGLLLFTPVAFLAWFPFVSE 1619

Query: 732  FQTRMLFNQAFSRGLQISRILGGPRKKEKSSSTDKE 768
            FQTRMLFNQAFSRGLQISRILGGPRKKEKSSSTDKE
Sbjct: 1620 FQTRMLFNQAFSRGLQISRILGGPRKKEKSSSTDKE 1655

BLAST of Spg013975 vs. NCBI nr
Match: XP_038898161.1 (callose synthase 1-like isoform X1 [Benincasa hispida] >XP_038898162.1 callose synthase 1-like isoform X1 [Benincasa hispida] >XP_038898163.1 callose synthase 1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 972.6 bits (2513), Expect = 2.0e-279
Identity = 495/516 (95.93%), Postives = 506/516 (98.06%), Query Frame = 0

Query: 252  RIFNDTIGSFDGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD 311
            ++ N +   F GFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD
Sbjct: 1422 KVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD 1481

Query: 312  IYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTVYVFLYGRLYLVLSGLEKQLSTHPAIK 371
            +YRLGHRFDFFRMLSCYFTT+GFYFST+LTVLTVYVFLYGRLYLVLSGLEK LSTHPAIK
Sbjct: 1482 MYRLGHRFDFFRMLSCYFTTVGFYFSTLLTVLTVYVFLYGRLYLVLSGLEKALSTHPAIK 1541

Query: 372  DNKSLQTALISQSAVQIGLLMALPMMVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGT 431
            DNKSLQTALISQSAVQIGLLMALPMMVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGT
Sbjct: 1542 DNKSLQTALISQSAVQIGLLMALPMMVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGT 1601

Query: 432  KTHYYGRTLLHGGAQYRGTGRGFVVFHAKFADNYRMYSRSHFVKGIELMILLIVYHILGV 491
            KTHYYGRTLLHGGA+YRGTGRGFVVFHAKFADNYRMYSRSHFVKGIELMILL+VYHILGV
Sbjct: 1602 KTHYYGRTLLHGGAEYRGTGRGFVVFHAKFADNYRMYSRSHFVKGIELMILLLVYHILGV 1661

Query: 492  SYRGVVAHVLITISIWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVSP 551
            SYRGVVAHVLITISIWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVSP
Sbjct: 1662 SYRGVVAHVLITISIWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVSP 1721

Query: 552  DKSWESWWEKEHEHLLYSGKRGIFVEILLSLRFFIFQYGLVYHLKIIDSQSFLVYGLSWV 611
            DKSWESWWEKEH+HL YSGKRGIF EILLSLRFFIFQYGLVYHLKIIDSQSFLVYGLSWV
Sbjct: 1722 DKSWESWWEKEHDHLKYSGKRGIFAEILLSLRFFIFQYGLVYHLKIIDSQSFLVYGLSWV 1781

Query: 612  VIISILLLMKAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAVPHMTIRDIILCL 671
            VIISILLLMKAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVA+PHMTIRDIILCL
Sbjct: 1782 VIISILLLMKAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAIPHMTIRDIILCL 1841

Query: 672  LAFLPTGWGLLLIAQACKRLIHHTALWGSVRALARSYEILMGLLLFTPVAFLAWFPFVSE 731
            LAFLPTGWGLLLIAQACK LIHHTALWGSVRALARSYEI+MGLLLFTPVAFLAWFPFVSE
Sbjct: 1842 LAFLPTGWGLLLIAQACKPLIHHTALWGSVRALARSYEIVMGLLLFTPVAFLAWFPFVSE 1901

Query: 732  FQTRMLFNQAFSRGLQISRILGGPRKKEKSSSTDKE 768
            FQTRMLFNQAFSRGLQISRILGGPRKKEKSSSTDKE
Sbjct: 1902 FQTRMLFNQAFSRGLQISRILGGPRKKEKSSSTDKE 1937

BLAST of Spg013975 vs. NCBI nr
Match: XP_022953403.1 (callose synthase 1 [Cucurbita moschata] >XP_022953404.1 callose synthase 1 [Cucurbita moschata] >XP_022953405.1 callose synthase 1 [Cucurbita moschata])

HSP 1 Score: 964.9 bits (2493), Expect = 4.1e-277
Identity = 491/516 (95.16%), Postives = 506/516 (98.06%), Query Frame = 0

Query: 252  RIFNDTIGSFDGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD 311
            ++ N +   F GFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD
Sbjct: 1420 KVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD 1479

Query: 312  IYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTVYVFLYGRLYLVLSGLEKQLSTHPAIK 371
            +YRLGHRFDFFRMLSCYFTT+GFYFSTMLTVLTVYVFLYGRLYLVLSGLEK LSTHPAIK
Sbjct: 1480 MYRLGHRFDFFRMLSCYFTTVGFYFSTMLTVLTVYVFLYGRLYLVLSGLEKALSTHPAIK 1539

Query: 372  DNKSLQTALISQSAVQIGLLMALPMMVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGT 431
            DNKSLQTALISQSAVQIGLLMALPMMVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGT
Sbjct: 1540 DNKSLQTALISQSAVQIGLLMALPMMVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGT 1599

Query: 432  KTHYYGRTLLHGGAQYRGTGRGFVVFHAKFADNYRMYSRSHFVKGIELMILLIVYHILGV 491
            KTHYYGRTLLHGGAQYRGTGRGFVVFHA+FADNYR+YSRSHFVKGIELMILL+VYHILGV
Sbjct: 1600 KTHYYGRTLLHGGAQYRGTGRGFVVFHARFADNYRLYSRSHFVKGIELMILLLVYHILGV 1659

Query: 492  SYRGVVAHVLITISIWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVSP 551
            SYRGVV HVLITISIWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVSP
Sbjct: 1660 SYRGVVVHVLITISIWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVSP 1719

Query: 552  DKSWESWWEKEHEHLLYSGKRGIFVEILLSLRFFIFQYGLVYHLKIIDSQSFLVYGLSWV 611
            D+SWESWWEKEHEHL YSGKRGIFVEILLSLRFFIFQYGLVYHL+II+SQSFLVYGLSWV
Sbjct: 1720 DRSWESWWEKEHEHLQYSGKRGIFVEILLSLRFFIFQYGLVYHLEIINSQSFLVYGLSWV 1779

Query: 612  VIISILLLMKAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAVPHMTIRDIILCL 671
            VIISILLLMKAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAVPHMTIRDIILCL
Sbjct: 1780 VIISILLLMKAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAVPHMTIRDIILCL 1839

Query: 672  LAFLPTGWGLLLIAQACKRLIHHTALWGSVRALARSYEILMGLLLFTPVAFLAWFPFVSE 731
            LAFLPTGWGLLLIAQAC+ LIH+TA+WGSVRALARSYEI+MGLLLFTPVAFLAWFPFVSE
Sbjct: 1840 LAFLPTGWGLLLIAQACRPLIHNTAIWGSVRALARSYEIVMGLLLFTPVAFLAWFPFVSE 1899

Query: 732  FQTRMLFNQAFSRGLQISRILGGPRKKEKSSSTDKE 768
            FQTRMLFNQAFSRGLQISRILGGPRKKEKSSSTDKE
Sbjct: 1900 FQTRMLFNQAFSRGLQISRILGGPRKKEKSSSTDKE 1935

BLAST of Spg013975 vs. NCBI nr
Match: KAG6575662.1 (Callose synthase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 964.9 bits (2493), Expect = 4.1e-277
Identity = 491/516 (95.16%), Postives = 506/516 (98.06%), Query Frame = 0

Query: 252  RIFNDTIGSFDGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD 311
            ++ N +   F GFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD
Sbjct: 1450 KVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD 1509

Query: 312  IYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTVYVFLYGRLYLVLSGLEKQLSTHPAIK 371
            +YRLGHRFDFFRMLSCYFTT+GFYFSTMLTVLTVYVFLYGRLYLVLSGLEK LSTHPAIK
Sbjct: 1510 MYRLGHRFDFFRMLSCYFTTVGFYFSTMLTVLTVYVFLYGRLYLVLSGLEKALSTHPAIK 1569

Query: 372  DNKSLQTALISQSAVQIGLLMALPMMVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGT 431
            DNKSLQTALISQSAVQIGLLMALPMMVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGT
Sbjct: 1570 DNKSLQTALISQSAVQIGLLMALPMMVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGT 1629

Query: 432  KTHYYGRTLLHGGAQYRGTGRGFVVFHAKFADNYRMYSRSHFVKGIELMILLIVYHILGV 491
            KTHYYGRTLLHGGAQYRGTGRGFVVFHA+FADNYR+YSRSHFVKGIELMILL+VYHILGV
Sbjct: 1630 KTHYYGRTLLHGGAQYRGTGRGFVVFHARFADNYRLYSRSHFVKGIELMILLLVYHILGV 1689

Query: 492  SYRGVVAHVLITISIWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVSP 551
            SYRGVV HVLITISIWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVSP
Sbjct: 1690 SYRGVVVHVLITISIWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVSP 1749

Query: 552  DKSWESWWEKEHEHLLYSGKRGIFVEILLSLRFFIFQYGLVYHLKIIDSQSFLVYGLSWV 611
            D+SWESWWEKEHEHL YSGKRGIFVEILLSLRFFIFQYGLVYHL+II+SQSFLVYGLSWV
Sbjct: 1750 DRSWESWWEKEHEHLQYSGKRGIFVEILLSLRFFIFQYGLVYHLEIINSQSFLVYGLSWV 1809

Query: 612  VIISILLLMKAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAVPHMTIRDIILCL 671
            VIISILLLMKAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAVPHMTIRDIILCL
Sbjct: 1810 VIISILLLMKAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAVPHMTIRDIILCL 1869

Query: 672  LAFLPTGWGLLLIAQACKRLIHHTALWGSVRALARSYEILMGLLLFTPVAFLAWFPFVSE 731
            LAFLPTGWGLLLIAQAC+ LIH+TA+WGSVRALARSYEI+MGLLLFTPVAFLAWFPFVSE
Sbjct: 1870 LAFLPTGWGLLLIAQACRPLIHNTAIWGSVRALARSYEIVMGLLLFTPVAFLAWFPFVSE 1929

Query: 732  FQTRMLFNQAFSRGLQISRILGGPRKKEKSSSTDKE 768
            FQTRMLFNQAFSRGLQISRILGGPRKKEKSSSTDKE
Sbjct: 1930 FQTRMLFNQAFSRGLQISRILGGPRKKEKSSSTDKE 1965

BLAST of Spg013975 vs. NCBI nr
Match: XP_022991842.1 (callose synthase 1 [Cucurbita maxima] >XP_022991843.1 callose synthase 1 [Cucurbita maxima] >XP_022991845.1 callose synthase 1 [Cucurbita maxima])

HSP 1 Score: 964.9 bits (2493), Expect = 4.1e-277
Identity = 491/516 (95.16%), Postives = 506/516 (98.06%), Query Frame = 0

Query: 252  RIFNDTIGSFDGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD 311
            ++ N +   F GFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD
Sbjct: 1420 KVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD 1479

Query: 312  IYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTVYVFLYGRLYLVLSGLEKQLSTHPAIK 371
            +YRLGHRFDFFRMLSCYFTT+GFYFSTMLTVLTVYVFLYGRLYLVLSGLEK LSTHPAIK
Sbjct: 1480 MYRLGHRFDFFRMLSCYFTTVGFYFSTMLTVLTVYVFLYGRLYLVLSGLEKALSTHPAIK 1539

Query: 372  DNKSLQTALISQSAVQIGLLMALPMMVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGT 431
            DNKSLQTALISQSAVQIGLLMALPMMVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGT
Sbjct: 1540 DNKSLQTALISQSAVQIGLLMALPMMVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGT 1599

Query: 432  KTHYYGRTLLHGGAQYRGTGRGFVVFHAKFADNYRMYSRSHFVKGIELMILLIVYHILGV 491
            KTHYYGRTLLHGGAQYRGTGRGFVVFHA+FADNYR+YSRSHFVKGIELMILL+VYHILGV
Sbjct: 1600 KTHYYGRTLLHGGAQYRGTGRGFVVFHARFADNYRLYSRSHFVKGIELMILLLVYHILGV 1659

Query: 492  SYRGVVAHVLITISIWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVSP 551
            SYRGVV HVLITISIWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVSP
Sbjct: 1660 SYRGVVVHVLITISIWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVSP 1719

Query: 552  DKSWESWWEKEHEHLLYSGKRGIFVEILLSLRFFIFQYGLVYHLKIIDSQSFLVYGLSWV 611
            D+SWESWWEKEHEHL YSGKRGIFVEILLSLRFFIFQYGLVYHL+II+SQSFLVYGLSWV
Sbjct: 1720 DRSWESWWEKEHEHLQYSGKRGIFVEILLSLRFFIFQYGLVYHLEIINSQSFLVYGLSWV 1779

Query: 612  VIISILLLMKAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAVPHMTIRDIILCL 671
            VIISILLLMKAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAVPHMTIRDIILCL
Sbjct: 1780 VIISILLLMKAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAVPHMTIRDIILCL 1839

Query: 672  LAFLPTGWGLLLIAQACKRLIHHTALWGSVRALARSYEILMGLLLFTPVAFLAWFPFVSE 731
            LAFLPTGWGLLLIAQAC+ LIH+TA+WGSVRALARSYEI+MGLLLFTPVAFLAWFPFVSE
Sbjct: 1840 LAFLPTGWGLLLIAQACRPLIHNTAIWGSVRALARSYEIVMGLLLFTPVAFLAWFPFVSE 1899

Query: 732  FQTRMLFNQAFSRGLQISRILGGPRKKEKSSSTDKE 768
            FQTRMLFNQAFSRGLQISRILGGPRKKEKSSSTDKE
Sbjct: 1900 FQTRMLFNQAFSRGLQISRILGGPRKKEKSSSTDKE 1935

BLAST of Spg013975 vs. ExPASy Swiss-Prot
Match: Q9LXT9 (Callose synthase 3 OS=Arabidopsis thaliana OX=3702 GN=CALS3 PE=3 SV=3)

HSP 1 Score: 809.7 bits (2090), Expect = 2.9e-233
Identity = 403/515 (78.25%), Postives = 457/515 (88.74%), Query Frame = 0

Query: 252  RIFNDTIGSFDGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD 311
            ++ N +   F GFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD
Sbjct: 1441 KVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD 1500

Query: 312  IYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTVYVFLYGRLYLVLSGLEKQLSTHPAIK 371
            IYRLGHRFDFFRM+SCYFTT+GFYFST++TVLTVY+FLYGRLYLVLSGLE+ LST   I+
Sbjct: 1501 IYRLGHRFDFFRMMSCYFTTVGFYFSTLITVLTVYIFLYGRLYLVLSGLEQGLSTQKGIR 1560

Query: 372  DNKSLQTALISQSAVQIGLLMALPMMVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGT 431
            DN  LQ AL SQS VQIG LMALPM++EIGLERGFR ALS+F+LMQLQLAPVFFTFSLGT
Sbjct: 1561 DNTPLQIALASQSFVQIGFLMALPMLMEIGLERGFRTALSEFVLMQLQLAPVFFTFSLGT 1620

Query: 432  KTHYYGRTLLHGGAQYRGTGRGFVVFHAKFADNYRMYSRSHFVKGIELMILLIVYHILGV 491
            KTHYYGRTLLHGGA+YR TGRGFVVFHAKFADNYR+YSRSHFVKG+E+M+LL+VY I G 
Sbjct: 1621 KTHYYGRTLLHGGAKYRSTGRGFVVFHAKFADNYRLYSRSHFVKGLEMMLLLVVYQIFGS 1680

Query: 492  SYRGVVAHVLITISIWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVSP 551
            +YRGV+A++LITIS+WFMVGTWLFAPFLFNPSGFEWQKIVDDWTDW KWI N GGIGV  
Sbjct: 1681 AYRGVLAYLLITISMWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWNKWINNIGGIGVPA 1740

Query: 552  DKSWESWWEKEHEHLLYSGKRGIFVEILLSLRFFIFQYGLVYHLKIID-SQSFLVYGLSW 611
            +KSWESWWE+E EHL YSGKRGI VEILL+LRFFI+QYGLVYHL I + +++FLVYG+SW
Sbjct: 1741 EKSWESWWEEEQEHLRYSGKRGIVVEILLALRFFIYQYGLVYHLTITEKTKNFLVYGVSW 1800

Query: 612  VVIISILLLMKAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAVPHMTIRDIILC 671
            +VI  IL +MK VSVGRR+FSASFQL+FRL +G IF+  +A  + L+ + HMTI+DII+C
Sbjct: 1801 LVIFLILFVMKTVSVGRRRFSASFQLMFRLIKGLIFMTFIAIIVILITLAHMTIQDIIVC 1860

Query: 672  LLAFLPTGWGLLLIAQACKRLIHHTALWGSVRALARSYEILMGLLLFTPVAFLAWFPFVS 731
            +LAF+PTGWG+LLIAQACK ++H    WGSVR LAR YEI+MGLLLFTPVAFLAWFPFVS
Sbjct: 1861 ILAFMPTGWGMLLIAQACKPVVHRAGFWGSVRTLARGYEIVMGLLLFTPVAFLAWFPFVS 1920

Query: 732  EFQTRMLFNQAFSRGLQISRILGGPRKKEKSSSTD 766
            EFQTRMLFNQAFSRGLQISRILGG RK   S + +
Sbjct: 1921 EFQTRMLFNQAFSRGLQISRILGGHRKDRSSRNKE 1955

BLAST of Spg013975 vs. ExPASy Swiss-Prot
Match: Q9AUE0 (Callose synthase 1 OS=Arabidopsis thaliana OX=3702 GN=CALS1 PE=1 SV=2)

HSP 1 Score: 789.3 bits (2037), Expect = 4.0e-227
Identity = 399/516 (77.33%), Postives = 445/516 (86.24%), Query Frame = 0

Query: 252  RIFNDTIGSFDGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD 311
            ++ N +   F GFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD
Sbjct: 1435 KVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD 1494

Query: 312  IYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTVYVFLYGRLYLVLSGLEKQLSTHPAIK 371
            +YRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTVYVFLYGRLYLVLSGLE+ LS+  A +
Sbjct: 1495 LYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTVYVFLYGRLYLVLSGLEEGLSSQRAFR 1554

Query: 372  DNKSLQTALISQSAVQIGLLMALPMMVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGT 431
            +NK L+ AL SQS VQIG LMALPMM+EIGLERGF  AL +F+LMQLQLA VFFTF LGT
Sbjct: 1555 NNKPLEAALASQSFVQIGFLMALPMMMEIGLERGFHNALIEFVLMQLQLASVFFTFQLGT 1614

Query: 432  KTHYYGRTLLHGGAQYRGTGRGFVVFHAKFADNYRMYSRSHFVKGIELMILLIVYHILGV 491
            KTHYYGRTL HGGA+YRGTGRGFVVFHAKFA+NYR YSRSHFVKGIELMILL+VY I G 
Sbjct: 1615 KTHYYGRTLFHGGAEYRGTGRGFVVFHAKFAENYRFYSRSHFVKGIELMILLLVYQIFGQ 1674

Query: 492  SYRGVVAHVLITISIWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVSP 551
            SYRGVV ++LIT+SIWFMV TWLFAPFLFNPSGFEWQKIVDDWTDW KWI NRGGIGV P
Sbjct: 1675 SYRGVVTYILITVSIWFMVVTWLFAPFLFNPSGFEWQKIVDDWTDWNKWIYNRGGIGVPP 1734

Query: 552  DKSWESWWEKEHEHLLYSGKRGIFVEILLSLRFFIFQYGLVYHLKII--DSQSFLVYGLS 611
            +KSWESWWEKE EHL +SG RGI +EI L+LRFFIFQYGLVYHL      +QSF VYG S
Sbjct: 1735 EKSWESWWEKELEHLRHSGVRGITLEIFLALRFFIFQYGLVYHLSTFKGKNQSFWVYGAS 1794

Query: 612  WVVIISILLLMKAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAVPHMTIRDIIL 671
            W VI+ ILL++K + VGRR+FS +FQLLFR+ +G +F+  VA  IT +A+P +TI+D+ +
Sbjct: 1795 WFVILFILLIVKGLGVGRRRFSTNFQLLFRIIKGLVFLTFVAILITFLALPLITIKDLFI 1854

Query: 672  CLLAFLPTGWGLLLIAQACKRLIHHTALWGSVRALARSYEILMGLLLFTPVAFLAWFPFV 731
            C+LAF+PTGWG+LLIAQACK LI    +W SVR LAR YEI+MGLLLFTPVAFLAWFPFV
Sbjct: 1855 CMLAFMPTGWGMLLIAQACKPLIQQLGIWSSVRTLARGYEIVMGLLLFTPVAFLAWFPFV 1914

Query: 732  SEFQTRMLFNQAFSRGLQISRILGGPRKKEKSSSTD 766
            SEFQTRMLFNQAFSRGLQISRILGG RK   S + +
Sbjct: 1915 SEFQTRMLFNQAFSRGLQISRILGGQRKDRSSKNKE 1950

BLAST of Spg013975 vs. ExPASy Swiss-Prot
Match: Q9SL03 (Callose synthase 2 OS=Arabidopsis thaliana OX=3702 GN=CALS2 PE=2 SV=3)

HSP 1 Score: 779.2 bits (2011), Expect = 4.2e-224
Identity = 395/516 (76.55%), Postives = 439/516 (85.08%), Query Frame = 0

Query: 252  RIFNDTIGSFDGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD 311
            ++ N +   F GFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD
Sbjct: 1435 KVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD 1494

Query: 312  IYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTVYVFLYGRLYLVLSGLEKQLSTHPAIK 371
            +YRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTVYVFLYGRLYLVLSGLE+ LS   A +
Sbjct: 1495 LYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTVYVFLYGRLYLVLSGLEEGLSNQKAFR 1554

Query: 372  DNKSLQTALISQSAVQIGLLMALPMMVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGT 431
             N  LQ AL SQS VQIG LMALPMM+EIGLERGF  AL DF+LMQLQLA VFFTF LGT
Sbjct: 1555 SNMPLQAALASQSFVQIGFLMALPMMMEIGLERGFHNALIDFVLMQLQLASVFFTFQLGT 1614

Query: 432  KTHYYGRTLLHGGAQYRGTGRGFVVFHAKFADNYRMYSRSHFVKGIELMILLIVYHILGV 491
            KTHYYGRTL HGGA+YRGTGRGFVVFHAKFA+NYR YSRSHFVKGIELMILL+VY I G 
Sbjct: 1615 KTHYYGRTLFHGGAEYRGTGRGFVVFHAKFAENYRFYSRSHFVKGIELMILLLVYQIFGH 1674

Query: 492  SYRGVVAHVLITISIWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVSP 551
            +YRGVV ++LIT+SIWFMV TWLFAPFLFNPSGFEWQKIVDDWTDW KWI NRGGIGV P
Sbjct: 1675 AYRGVVTYILITVSIWFMVVTWLFAPFLFNPSGFEWQKIVDDWTDWNKWIYNRGGIGVPP 1734

Query: 552  DKSWESWWEKEHEHLLYSGKRGIFVEILLSLRFFIFQYGLVYHLKII--DSQSFLVYGLS 611
            +KSWESWWEKE  HL +SGKRGI +EI+L+LRFFIFQYGLVY L     ++QS  +YG S
Sbjct: 1735 EKSWESWWEKEIGHLRHSGKRGIILEIVLALRFFIFQYGLVYQLSTFKQENQSLWIYGAS 1794

Query: 612  WVVIISILLLMKAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAVPHMTIRDIIL 671
            W VI+ ILL++K + VGR++FS +FQLLFR+ +GF+F+  +   IT +A+  +T +DI L
Sbjct: 1795 WFVILFILLIVKGLGVGRQRFSTNFQLLFRIIKGFVFLTFLGLLITFLALRFLTPKDIFL 1854

Query: 672  CLLAFLPTGWGLLLIAQACKRLIHHTALWGSVRALARSYEILMGLLLFTPVAFLAWFPFV 731
            C+LAF+PTGWG+LLIAQACK LI     W SVR LAR YEILMGLLLFTPVAFLAWFPFV
Sbjct: 1855 CMLAFMPTGWGMLLIAQACKPLIQRLGFWSSVRTLARGYEILMGLLLFTPVAFLAWFPFV 1914

Query: 732  SEFQTRMLFNQAFSRGLQISRILGGPRKKEKSSSTD 766
            SEFQTRMLFNQAFSRGLQISRILGG RK   S + +
Sbjct: 1915 SEFQTRMLFNQAFSRGLQISRILGGQRKDRSSKNKE 1950

BLAST of Spg013975 vs. ExPASy Swiss-Prot
Match: Q3B724 (Callose synthase 5 OS=Arabidopsis thaliana OX=3702 GN=CALS5 PE=1 SV=1)

HSP 1 Score: 674.5 bits (1739), Expect = 1.4e-192
Identity = 340/505 (67.33%), Postives = 406/505 (80.40%), Query Frame = 0

Query: 261  FDGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRDIYRLGHRFD 320
            F GFNSTLR GNVTHHEYIQVGKGRDVGLNQIS+FEAK+A GNGEQTLSRD+YRLGHRFD
Sbjct: 1419 FAGFNSTLRRGNVTHHEYIQVGKGRDVGLNQISLFEAKVACGNGEQTLSRDLYRLGHRFD 1478

Query: 321  FFRMLSCYFTTIGFYFSTMLTVLTVYVFLYGRLYLVLSGLEKQLSTHPAIKDNKSLQTAL 380
            FFRM+SCYFTT+GFY S+M+ VLTVY FLYGRLYL LSG+E+ +    A K + SL+ A+
Sbjct: 1479 FFRMMSCYFTTVGFYISSMIVVLTVYAFLYGRLYLSLSGVEEAIVKFAAAKGDSSLKAAM 1538

Query: 381  ISQSAVQIGLLMALPMMVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTL 440
             SQS VQ+GLLM LPM++EIGLERGFR ALSD I+MQLQLAPVFFTFSLGTK HYYGRT+
Sbjct: 1539 ASQSVVQLGLLMTLPMVMEIGLERGFRTALSDLIIMQLQLAPVFFTFSLGTKVHYYGRTI 1598

Query: 441  LHGGAQYRGTGRGFVVFHAKFADNYRMYSRSHFVKGIELMILLIVYHILGVSYRGVVAHV 500
            LHGG++YR TGRGFVV H KFA+NYRMYSRSHFVKG+ELM+LLI Y I G +    V + 
Sbjct: 1599 LHGGSKYRATGRGFVVKHEKFAENYRMYSRSHFVKGMELMVLLICYRIYGKAAEDSVGYA 1658

Query: 501  LITISIWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVSPDKSWESWWE 560
            L+  S WF+VG+WLFAPF FNPSGFEWQKIVDDW DW KWI +RGGIGV  +KSWESWWE
Sbjct: 1659 LVMGSTWFLVGSWLFAPFFFNPSGFEWQKIVDDWDDWNKWISSRGGIGVPANKSWESWWE 1718

Query: 561  KEHEHLLYSGKRGIFVEILLSLRFFIFQYGLVYHLKII------DSQSFLVYGLSWVVII 620
            +E EHLL+SG  G F EI LSLR+FI+QYG+VY L +          S +VYGLSW+VI+
Sbjct: 1719 EEQEHLLHSGFFGKFWEIFLSLRYFIYQYGIVYQLNLTKESRMGKQHSIIVYGLSWLVIV 1778

Query: 621  SILLLMKAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAVPHMTIRDIILCLLAF 680
            ++++++K VS+GR+KFSA FQL+FRL + F+FI  V     L     +T+ DI+  LLAF
Sbjct: 1779 AVMIVLKIVSMGRKKFSADFQLMFRLLKLFLFIGSVVIVGMLFHFLKLTVGDIMQSLLAF 1838

Query: 681  LPTGWGLLLIAQACKRLIHHTALWGSVRALARSYEILMGLLLFTPVAFLAWFPFVSEFQT 740
            LPTGW LL I+Q  + L+    +WGSV+ALAR YE +MG+++F PV  LAWFPFVSEFQT
Sbjct: 1839 LPTGWALLQISQVARPLMKTVGMWGSVKALARGYEYIMGVVIFMPVTVLAWFPFVSEFQT 1898

Query: 741  RMLFNQAFSRGLQISRILGGPRKKE 760
            R+LFNQAFSRGLQI RIL G +K++
Sbjct: 1899 RLLFNQAFSRGLQIQRILAGGKKQK 1923

BLAST of Spg013975 vs. ExPASy Swiss-Prot
Match: Q9SHJ3 (Callose synthase 7 OS=Arabidopsis thaliana OX=3702 GN=CALS7 PE=3 SV=3)

HSP 1 Score: 671.4 bits (1731), Expect = 1.2e-191
Identity = 329/513 (64.13%), Postives = 411/513 (80.12%), Query Frame = 0

Query: 252  RIFNDTIGSFDGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD 311
            +I N +   F G+NSTLR G VTHHEYIQ GKGRDVG+NQIS FEAK+ANGNGEQTLSRD
Sbjct: 1420 KIINLSEDIFAGYNSTLRGGYVTHHEYIQAGKGRDVGMNQISFFEAKVANGNGEQTLSRD 1479

Query: 312  IYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTVYVFLYGRLYLVLSGLEKQLSTHPAIK 371
            +YRLG RFDF+RMLS YFTT+GFYFS+M+TVLTVYVFLYGRLYLVLSGLEK +    ++ 
Sbjct: 1480 VYRLGRRFDFYRMLSFYFTTVGFYFSSMITVLTVYVFLYGRLYLVLSGLEKNILQSASVH 1539

Query: 372  DNKSLQTALISQSAVQIGLLMALPMMVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGT 431
            ++ +L+ AL +QS  Q+G LM LPM++EIGLE+GFR AL DFI+MQLQLA VFFTF LGT
Sbjct: 1540 ESNALEQALAAQSVFQLGFLMVLPMVMEIGLEKGFRTALGDFIIMQLQLASVFFTFQLGT 1599

Query: 432  KTHYYGRTLLHGGAQYRGTGRGFVVFHAKFADNYRMYSRSHFVKGIELMILLIVYHILGV 491
            K HY+GRT+LHGG++YR TGRGFVVFHAKFA+NYR+YSRSHFVKG+EL+ILL+VY + G 
Sbjct: 1600 KAHYFGRTILHGGSKYRATGRGFVVFHAKFAENYRLYSRSHFVKGLELVILLVVYQVYGT 1659

Query: 492  SYRGVVAHVLITISIWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVSP 551
            SYR    ++ IT S+WF+V +WLFAPF+FNPSGFEWQK VDDWTDWK+W+ NRGGIG+  
Sbjct: 1660 SYRSSSTYMYITFSMWFLVTSWLFAPFIFNPSGFEWQKTVDDWTDWKRWMGNRGGIGIVL 1719

Query: 552  DKSWESWWEKEHEHLLYSGKRGIFVEILLSLRFFIFQYGLVYHLKIIDSQ-SFLVYGLSW 611
            DKSWESWW+ E EHL ++  RG  +EILL+LRF ++QYG+VYHL I     +FLVYGLSW
Sbjct: 1720 DKSWESWWDIEQEHLKHTNLRGRVLEILLALRFLLYQYGIVYHLNIARRHTTFLVYGLSW 1779

Query: 612  VVIISILLLMKAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAVPHMTIRDIILC 671
             +++S+LL++K VS+GRRKF   FQ++FR+ +  +F+  ++    L  V  +TI D+   
Sbjct: 1780 AILLSVLLVLKMVSMGRRKFGTDFQVMFRILKALLFLGFLSVMTVLFVVCGLTISDLFAS 1839

Query: 672  LLAFLPTGWGLLLIAQACKRLIHHTALWGSVRALARSYEILMGLLLFTPVAFLAWFPFVS 731
            +LAFLPTGW +LLI QA + +      W SV+ L R+YE +MGL++FTP+A L+WFPFVS
Sbjct: 1840 ILAFLPTGWAILLIGQALRSVFKGLGFWDSVKELGRAYEYIMGLVIFTPIAVLSWFPFVS 1899

Query: 732  EFQTRMLFNQAFSRGLQISRILGGPRKKEKSSS 764
            EFQTR+LFNQAFSRGLQIS IL G + KE  S+
Sbjct: 1900 EFQTRLLFNQAFSRGLQISMILAGKKDKETPST 1932

BLAST of Spg013975 vs. ExPASy TrEMBL
Match: A0A6J1JU28 (1,3-beta-glucan synthase OS=Cucurbita maxima OX=3661 GN=LOC111488369 PE=3 SV=1)

HSP 1 Score: 964.9 bits (2493), Expect = 2.0e-277
Identity = 491/516 (95.16%), Postives = 506/516 (98.06%), Query Frame = 0

Query: 252  RIFNDTIGSFDGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD 311
            ++ N +   F GFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD
Sbjct: 1420 KVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD 1479

Query: 312  IYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTVYVFLYGRLYLVLSGLEKQLSTHPAIK 371
            +YRLGHRFDFFRMLSCYFTT+GFYFSTMLTVLTVYVFLYGRLYLVLSGLEK LSTHPAIK
Sbjct: 1480 MYRLGHRFDFFRMLSCYFTTVGFYFSTMLTVLTVYVFLYGRLYLVLSGLEKALSTHPAIK 1539

Query: 372  DNKSLQTALISQSAVQIGLLMALPMMVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGT 431
            DNKSLQTALISQSAVQIGLLMALPMMVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGT
Sbjct: 1540 DNKSLQTALISQSAVQIGLLMALPMMVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGT 1599

Query: 432  KTHYYGRTLLHGGAQYRGTGRGFVVFHAKFADNYRMYSRSHFVKGIELMILLIVYHILGV 491
            KTHYYGRTLLHGGAQYRGTGRGFVVFHA+FADNYR+YSRSHFVKGIELMILL+VYHILGV
Sbjct: 1600 KTHYYGRTLLHGGAQYRGTGRGFVVFHARFADNYRLYSRSHFVKGIELMILLLVYHILGV 1659

Query: 492  SYRGVVAHVLITISIWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVSP 551
            SYRGVV HVLITISIWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVSP
Sbjct: 1660 SYRGVVVHVLITISIWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVSP 1719

Query: 552  DKSWESWWEKEHEHLLYSGKRGIFVEILLSLRFFIFQYGLVYHLKIIDSQSFLVYGLSWV 611
            D+SWESWWEKEHEHL YSGKRGIFVEILLSLRFFIFQYGLVYHL+II+SQSFLVYGLSWV
Sbjct: 1720 DRSWESWWEKEHEHLQYSGKRGIFVEILLSLRFFIFQYGLVYHLEIINSQSFLVYGLSWV 1779

Query: 612  VIISILLLMKAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAVPHMTIRDIILCL 671
            VIISILLLMKAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAVPHMTIRDIILCL
Sbjct: 1780 VIISILLLMKAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAVPHMTIRDIILCL 1839

Query: 672  LAFLPTGWGLLLIAQACKRLIHHTALWGSVRALARSYEILMGLLLFTPVAFLAWFPFVSE 731
            LAFLPTGWGLLLIAQAC+ LIH+TA+WGSVRALARSYEI+MGLLLFTPVAFLAWFPFVSE
Sbjct: 1840 LAFLPTGWGLLLIAQACRPLIHNTAIWGSVRALARSYEIVMGLLLFTPVAFLAWFPFVSE 1899

Query: 732  FQTRMLFNQAFSRGLQISRILGGPRKKEKSSSTDKE 768
            FQTRMLFNQAFSRGLQISRILGGPRKKEKSSSTDKE
Sbjct: 1900 FQTRMLFNQAFSRGLQISRILGGPRKKEKSSSTDKE 1935

BLAST of Spg013975 vs. ExPASy TrEMBL
Match: A0A6J1GN91 (1,3-beta-glucan synthase OS=Cucurbita moschata OX=3662 GN=LOC111455968 PE=3 SV=1)

HSP 1 Score: 964.9 bits (2493), Expect = 2.0e-277
Identity = 491/516 (95.16%), Postives = 506/516 (98.06%), Query Frame = 0

Query: 252  RIFNDTIGSFDGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD 311
            ++ N +   F GFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD
Sbjct: 1420 KVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD 1479

Query: 312  IYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTVYVFLYGRLYLVLSGLEKQLSTHPAIK 371
            +YRLGHRFDFFRMLSCYFTT+GFYFSTMLTVLTVYVFLYGRLYLVLSGLEK LSTHPAIK
Sbjct: 1480 MYRLGHRFDFFRMLSCYFTTVGFYFSTMLTVLTVYVFLYGRLYLVLSGLEKALSTHPAIK 1539

Query: 372  DNKSLQTALISQSAVQIGLLMALPMMVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGT 431
            DNKSLQTALISQSAVQIGLLMALPMMVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGT
Sbjct: 1540 DNKSLQTALISQSAVQIGLLMALPMMVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGT 1599

Query: 432  KTHYYGRTLLHGGAQYRGTGRGFVVFHAKFADNYRMYSRSHFVKGIELMILLIVYHILGV 491
            KTHYYGRTLLHGGAQYRGTGRGFVVFHA+FADNYR+YSRSHFVKGIELMILL+VYHILGV
Sbjct: 1600 KTHYYGRTLLHGGAQYRGTGRGFVVFHARFADNYRLYSRSHFVKGIELMILLLVYHILGV 1659

Query: 492  SYRGVVAHVLITISIWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVSP 551
            SYRGVV HVLITISIWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVSP
Sbjct: 1660 SYRGVVVHVLITISIWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVSP 1719

Query: 552  DKSWESWWEKEHEHLLYSGKRGIFVEILLSLRFFIFQYGLVYHLKIIDSQSFLVYGLSWV 611
            D+SWESWWEKEHEHL YSGKRGIFVEILLSLRFFIFQYGLVYHL+II+SQSFLVYGLSWV
Sbjct: 1720 DRSWESWWEKEHEHLQYSGKRGIFVEILLSLRFFIFQYGLVYHLEIINSQSFLVYGLSWV 1779

Query: 612  VIISILLLMKAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAVPHMTIRDIILCL 671
            VIISILLLMKAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAVPHMTIRDIILCL
Sbjct: 1780 VIISILLLMKAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAVPHMTIRDIILCL 1839

Query: 672  LAFLPTGWGLLLIAQACKRLIHHTALWGSVRALARSYEILMGLLLFTPVAFLAWFPFVSE 731
            LAFLPTGWGLLLIAQAC+ LIH+TA+WGSVRALARSYEI+MGLLLFTPVAFLAWFPFVSE
Sbjct: 1840 LAFLPTGWGLLLIAQACRPLIHNTAIWGSVRALARSYEIVMGLLLFTPVAFLAWFPFVSE 1899

Query: 732  FQTRMLFNQAFSRGLQISRILGGPRKKEKSSSTDKE 768
            FQTRMLFNQAFSRGLQISRILGGPRKKEKSSSTDKE
Sbjct: 1900 FQTRMLFNQAFSRGLQISRILGGPRKKEKSSSTDKE 1935

BLAST of Spg013975 vs. ExPASy TrEMBL
Match: A0A0A0K8F7 (1,3-beta-glucan synthase OS=Cucumis sativus OX=3659 GN=Csa_7G429550 PE=3 SV=1)

HSP 1 Score: 961.4 bits (2484), Expect = 2.2e-276
Identity = 487/516 (94.38%), Postives = 505/516 (97.87%), Query Frame = 0

Query: 252  RIFNDTIGSFDGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD 311
            ++ N +   F GFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD
Sbjct: 1432 KVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD 1491

Query: 312  IYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTVYVFLYGRLYLVLSGLEKQLSTHPAIK 371
            +YRLGHRFDFFRMLSCYFTT+GFYFSTMLTVLTVYVFLYGRLYLVLSGLE+ LSTHPAIK
Sbjct: 1492 MYRLGHRFDFFRMLSCYFTTVGFYFSTMLTVLTVYVFLYGRLYLVLSGLERALSTHPAIK 1551

Query: 372  DNKSLQTALISQSAVQIGLLMALPMMVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGT 431
            DNKSLQTALISQSAVQIGLLMALPMMVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGT
Sbjct: 1552 DNKSLQTALISQSAVQIGLLMALPMMVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGT 1611

Query: 432  KTHYYGRTLLHGGAQYRGTGRGFVVFHAKFADNYRMYSRSHFVKGIELMILLIVYHILGV 491
            KTHYYGRTLLHGGA+YRGTGRGFVVFHAKFA+NYRMYSRSHFVKGIELMILL+VYHILGV
Sbjct: 1612 KTHYYGRTLLHGGAEYRGTGRGFVVFHAKFAENYRMYSRSHFVKGIELMILLLVYHILGV 1671

Query: 492  SYRGVVAHVLITISIWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVSP 551
            SYRGVVAHVLITISIWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVSP
Sbjct: 1672 SYRGVVAHVLITISIWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVSP 1731

Query: 552  DKSWESWWEKEHEHLLYSGKRGIFVEILLSLRFFIFQYGLVYHLKIIDSQSFLVYGLSWV 611
            DKSWESWWEKEH+HL +SGKRGIFVEILLS+RFFIFQYGLVYHLKII+SQSFLVYGLSWV
Sbjct: 1732 DKSWESWWEKEHDHLKFSGKRGIFVEILLSIRFFIFQYGLVYHLKIIESQSFLVYGLSWV 1791

Query: 612  VIISILLLMKAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAVPHMTIRDIILCL 671
            VIISILLLMKAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVA+PHMTIRDIILC+
Sbjct: 1792 VIISILLLMKAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAIPHMTIRDIILCI 1851

Query: 672  LAFLPTGWGLLLIAQACKRLIHHTALWGSVRALARSYEILMGLLLFTPVAFLAWFPFVSE 731
            LAFLPTGWGLLLIAQACK LIH T LWGSVRALARSYEI+MGLLLFTP+AFLAWFPFVSE
Sbjct: 1852 LAFLPTGWGLLLIAQACKPLIHQTPLWGSVRALARSYEIVMGLLLFTPIAFLAWFPFVSE 1911

Query: 732  FQTRMLFNQAFSRGLQISRILGGPRKKEKSSSTDKE 768
            FQTRMLFNQAFSRGLQISRILGGPRKKEKSS+TDKE
Sbjct: 1912 FQTRMLFNQAFSRGLQISRILGGPRKKEKSSTTDKE 1947

BLAST of Spg013975 vs. ExPASy TrEMBL
Match: A0A5A7UU13 (1,3-beta-glucan synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G001200 PE=3 SV=1)

HSP 1 Score: 959.1 bits (2478), Expect = 1.1e-275
Identity = 486/504 (96.43%), Postives = 499/504 (99.01%), Query Frame = 0

Query: 264  FNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRDIYRLGHRFDFFR 323
            FNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD+YRLGHRFDFFR
Sbjct: 1480 FNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRDMYRLGHRFDFFR 1539

Query: 324  MLSCYFTTIGFYFSTMLTVLTVYVFLYGRLYLVLSGLEKQLSTHPAIKDNKSLQTALISQ 383
            MLSCYFTT+GFYFSTMLTVLTVYVFLYGRLYLVLSGLEK LSTHPAIKDNKSLQTALISQ
Sbjct: 1540 MLSCYFTTVGFYFSTMLTVLTVYVFLYGRLYLVLSGLEKALSTHPAIKDNKSLQTALISQ 1599

Query: 384  SAVQIGLLMALPMMVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHG 443
            SAVQIGLLMALPMMVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHG
Sbjct: 1600 SAVQIGLLMALPMMVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGTKTHYYGRTLLHG 1659

Query: 444  GAQYRGTGRGFVVFHAKFADNYRMYSRSHFVKGIELMILLIVYHILGVSYRGVVAHVLIT 503
            GA+YRGTGRGFVVFHAKFA+NYRMYSRSHFVKGIELMILL+VYHILGVSYRGVVAHVLIT
Sbjct: 1660 GAEYRGTGRGFVVFHAKFAENYRMYSRSHFVKGIELMILLLVYHILGVSYRGVVAHVLIT 1719

Query: 504  ISIWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVSPDKSWESWWEKEH 563
            ISIWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVSPDKSWESWWEKEH
Sbjct: 1720 ISIWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVSPDKSWESWWEKEH 1779

Query: 564  EHLLYSGKRGIFVEILLSLRFFIFQYGLVYHLKIIDSQSFLVYGLSWVVIISILLLMKAV 623
            EHL +SGKRGIFVEILLS+RFFIFQYGLVYHLKII+SQSFLVYGLSWVVIISILLLMKAV
Sbjct: 1780 EHLKFSGKRGIFVEILLSIRFFIFQYGLVYHLKIIESQSFLVYGLSWVVIISILLLMKAV 1839

Query: 624  SVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAVPHMTIRDIILCLLAFLPTGWGLLL 683
            SVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVA+PHMTIRDIILC+LAFLPTGWGLLL
Sbjct: 1840 SVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAIPHMTIRDIILCILAFLPTGWGLLL 1899

Query: 684  IAQACKRLIHHTALWGSVRALARSYEILMGLLLFTPVAFLAWFPFVSEFQTRMLFNQAFS 743
            IAQACK LIH T LWGSVRALARSYEI+MGLLLFTP+AFLAWFPFVSEFQTRMLFNQAFS
Sbjct: 1900 IAQACKPLIHQTPLWGSVRALARSYEIVMGLLLFTPIAFLAWFPFVSEFQTRMLFNQAFS 1959

Query: 744  RGLQISRILGGPRKKEKSSSTDKE 768
            RGLQISRILGGPRKKEKSS+TDKE
Sbjct: 1960 RGLQISRILGGPRKKEKSSTTDKE 1983

BLAST of Spg013975 vs. ExPASy TrEMBL
Match: A0A6J1CXR8 (1,3-beta-glucan synthase OS=Momordica charantia OX=3673 GN=LOC111015422 PE=3 SV=1)

HSP 1 Score: 951.4 bits (2458), Expect = 2.3e-273
Identity = 481/516 (93.22%), Postives = 500/516 (96.90%), Query Frame = 0

Query: 252  RIFNDTIGSFDGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD 311
            ++ N +   F GFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD
Sbjct: 1309 KVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD 1368

Query: 312  IYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTVYVFLYGRLYLVLSGLEKQLSTHPAIK 371
            IYRLGHRFDFFRMLSCYFTT+GFYFSTMLTVLTVY+FLYGRLYLVLSGLEK LSTHPAIK
Sbjct: 1369 IYRLGHRFDFFRMLSCYFTTVGFYFSTMLTVLTVYIFLYGRLYLVLSGLEKALSTHPAIK 1428

Query: 372  DNKSLQTALISQSAVQIGLLMALPMMVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGT 431
            DNKSLQTALISQSAVQIGLLMALPM+VEIGLERGFRAALSDFILMQLQLAPVFFTFSLGT
Sbjct: 1429 DNKSLQTALISQSAVQIGLLMALPMVVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGT 1488

Query: 432  KTHYYGRTLLHGGAQYRGTGRGFVVFHAKFADNYRMYSRSHFVKGIELMILLIVYHILGV 491
            KTHYYGRTLLHGGAQYRGTGR FVVFHAKFADNYR+YSRSHFVKGIELMILL+VYHILGV
Sbjct: 1489 KTHYYGRTLLHGGAQYRGTGRRFVVFHAKFADNYRLYSRSHFVKGIELMILLLVYHILGV 1548

Query: 492  SYRGVVAHVLITISIWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVSP 551
            SYRG V H+LIT+SIWFM+GTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGV+P
Sbjct: 1549 SYRGAVVHILITVSIWFMIGTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVAP 1608

Query: 552  DKSWESWWEKEHEHLLYSGKRGIFVEILLSLRFFIFQYGLVYHLKIIDSQSFLVYGLSWV 611
            DKSWESWWEKE EHL +SGKRGIF EILLSLRFFIFQYGLVYHL+II+SQSFLVYGLSWV
Sbjct: 1609 DKSWESWWEKEQEHLHHSGKRGIFTEILLSLRFFIFQYGLVYHLEIINSQSFLVYGLSWV 1668

Query: 612  VIISILLLMKAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAVPHMTIRDIILCL 671
            VIISILLLMKAVSVGRR+FSASFQLLFRLAEGF+FIIC+AGFITLVAVPHMTIRDIILC+
Sbjct: 1669 VIISILLLMKAVSVGRRRFSASFQLLFRLAEGFVFIICLAGFITLVAVPHMTIRDIILCI 1728

Query: 672  LAFLPTGWGLLLIAQACKRLIHHTALWGSVRALARSYEILMGLLLFTPVAFLAWFPFVSE 731
            LAFLPTGWGLLLIAQACK LIHHT LWGSVRALARSYEILMGLLLFTPVAFLAWFPFVSE
Sbjct: 1729 LAFLPTGWGLLLIAQACKPLIHHTGLWGSVRALARSYEILMGLLLFTPVAFLAWFPFVSE 1788

Query: 732  FQTRMLFNQAFSRGLQISRILGGPRKKEKSSSTDKE 768
            FQTRMLFNQAFSRGLQISRILGGPRKKEKSSS DKE
Sbjct: 1789 FQTRMLFNQAFSRGLQISRILGGPRKKEKSSSNDKE 1824

BLAST of Spg013975 vs. TAIR 10
Match: AT5G13000.1 (glucan synthase-like 12 )

HSP 1 Score: 809.7 bits (2090), Expect = 2.0e-234
Identity = 403/515 (78.25%), Postives = 457/515 (88.74%), Query Frame = 0

Query: 252  RIFNDTIGSFDGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD 311
            ++ N +   F GFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD
Sbjct: 1441 KVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD 1500

Query: 312  IYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTVYVFLYGRLYLVLSGLEKQLSTHPAIK 371
            IYRLGHRFDFFRM+SCYFTT+GFYFST++TVLTVY+FLYGRLYLVLSGLE+ LST   I+
Sbjct: 1501 IYRLGHRFDFFRMMSCYFTTVGFYFSTLITVLTVYIFLYGRLYLVLSGLEQGLSTQKGIR 1560

Query: 372  DNKSLQTALISQSAVQIGLLMALPMMVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGT 431
            DN  LQ AL SQS VQIG LMALPM++EIGLERGFR ALS+F+LMQLQLAPVFFTFSLGT
Sbjct: 1561 DNTPLQIALASQSFVQIGFLMALPMLMEIGLERGFRTALSEFVLMQLQLAPVFFTFSLGT 1620

Query: 432  KTHYYGRTLLHGGAQYRGTGRGFVVFHAKFADNYRMYSRSHFVKGIELMILLIVYHILGV 491
            KTHYYGRTLLHGGA+YR TGRGFVVFHAKFADNYR+YSRSHFVKG+E+M+LL+VY I G 
Sbjct: 1621 KTHYYGRTLLHGGAKYRSTGRGFVVFHAKFADNYRLYSRSHFVKGLEMMLLLVVYQIFGS 1680

Query: 492  SYRGVVAHVLITISIWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVSP 551
            +YRGV+A++LITIS+WFMVGTWLFAPFLFNPSGFEWQKIVDDWTDW KWI N GGIGV  
Sbjct: 1681 AYRGVLAYLLITISMWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWNKWINNIGGIGVPA 1740

Query: 552  DKSWESWWEKEHEHLLYSGKRGIFVEILLSLRFFIFQYGLVYHLKIID-SQSFLVYGLSW 611
            +KSWESWWE+E EHL YSGKRGI VEILL+LRFFI+QYGLVYHL I + +++FLVYG+SW
Sbjct: 1741 EKSWESWWEEEQEHLRYSGKRGIVVEILLALRFFIYQYGLVYHLTITEKTKNFLVYGVSW 1800

Query: 612  VVIISILLLMKAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAVPHMTIRDIILC 671
            +VI  IL +MK VSVGRR+FSASFQL+FRL +G IF+  +A  + L+ + HMTI+DII+C
Sbjct: 1801 LVIFLILFVMKTVSVGRRRFSASFQLMFRLIKGLIFMTFIAIIVILITLAHMTIQDIIVC 1860

Query: 672  LLAFLPTGWGLLLIAQACKRLIHHTALWGSVRALARSYEILMGLLLFTPVAFLAWFPFVS 731
            +LAF+PTGWG+LLIAQACK ++H    WGSVR LAR YEI+MGLLLFTPVAFLAWFPFVS
Sbjct: 1861 ILAFMPTGWGMLLIAQACKPVVHRAGFWGSVRTLARGYEIVMGLLLFTPVAFLAWFPFVS 1920

Query: 732  EFQTRMLFNQAFSRGLQISRILGGPRKKEKSSSTD 766
            EFQTRMLFNQAFSRGLQISRILGG RK   S + +
Sbjct: 1921 EFQTRMLFNQAFSRGLQISRILGGHRKDRSSRNKE 1955

BLAST of Spg013975 vs. TAIR 10
Match: AT1G05570.1 (callose synthase 1 )

HSP 1 Score: 789.3 bits (2037), Expect = 2.8e-228
Identity = 399/516 (77.33%), Postives = 445/516 (86.24%), Query Frame = 0

Query: 252  RIFNDTIGSFDGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD 311
            ++ N +   F GFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD
Sbjct: 1435 KVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD 1494

Query: 312  IYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTVYVFLYGRLYLVLSGLEKQLSTHPAIK 371
            +YRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTVYVFLYGRLYLVLSGLE+ LS+  A +
Sbjct: 1495 LYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTVYVFLYGRLYLVLSGLEEGLSSQRAFR 1554

Query: 372  DNKSLQTALISQSAVQIGLLMALPMMVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGT 431
            +NK L+ AL SQS VQIG LMALPMM+EIGLERGF  AL +F+LMQLQLA VFFTF LGT
Sbjct: 1555 NNKPLEAALASQSFVQIGFLMALPMMMEIGLERGFHNALIEFVLMQLQLASVFFTFQLGT 1614

Query: 432  KTHYYGRTLLHGGAQYRGTGRGFVVFHAKFADNYRMYSRSHFVKGIELMILLIVYHILGV 491
            KTHYYGRTL HGGA+YRGTGRGFVVFHAKFA+NYR YSRSHFVKGIELMILL+VY I G 
Sbjct: 1615 KTHYYGRTLFHGGAEYRGTGRGFVVFHAKFAENYRFYSRSHFVKGIELMILLLVYQIFGQ 1674

Query: 492  SYRGVVAHVLITISIWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVSP 551
            SYRGVV ++LIT+SIWFMV TWLFAPFLFNPSGFEWQKIVDDWTDW KWI NRGGIGV P
Sbjct: 1675 SYRGVVTYILITVSIWFMVVTWLFAPFLFNPSGFEWQKIVDDWTDWNKWIYNRGGIGVPP 1734

Query: 552  DKSWESWWEKEHEHLLYSGKRGIFVEILLSLRFFIFQYGLVYHLKII--DSQSFLVYGLS 611
            +KSWESWWEKE EHL +SG RGI +EI L+LRFFIFQYGLVYHL      +QSF VYG S
Sbjct: 1735 EKSWESWWEKELEHLRHSGVRGITLEIFLALRFFIFQYGLVYHLSTFKGKNQSFWVYGAS 1794

Query: 612  WVVIISILLLMKAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAVPHMTIRDIIL 671
            W VI+ ILL++K + VGRR+FS +FQLLFR+ +G +F+  VA  IT +A+P +TI+D+ +
Sbjct: 1795 WFVILFILLIVKGLGVGRRRFSTNFQLLFRIIKGLVFLTFVAILITFLALPLITIKDLFI 1854

Query: 672  CLLAFLPTGWGLLLIAQACKRLIHHTALWGSVRALARSYEILMGLLLFTPVAFLAWFPFV 731
            C+LAF+PTGWG+LLIAQACK LI    +W SVR LAR YEI+MGLLLFTPVAFLAWFPFV
Sbjct: 1855 CMLAFMPTGWGMLLIAQACKPLIQQLGIWSSVRTLARGYEIVMGLLLFTPVAFLAWFPFV 1914

Query: 732  SEFQTRMLFNQAFSRGLQISRILGGPRKKEKSSSTD 766
            SEFQTRMLFNQAFSRGLQISRILGG RK   S + +
Sbjct: 1915 SEFQTRMLFNQAFSRGLQISRILGGQRKDRSSKNKE 1950

BLAST of Spg013975 vs. TAIR 10
Match: AT1G05570.2 (callose synthase 1 )

HSP 1 Score: 789.3 bits (2037), Expect = 2.8e-228
Identity = 399/516 (77.33%), Postives = 445/516 (86.24%), Query Frame = 0

Query: 252  RIFNDTIGSFDGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD 311
            ++ N +   F GFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD
Sbjct: 1394 KVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD 1453

Query: 312  IYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTVYVFLYGRLYLVLSGLEKQLSTHPAIK 371
            +YRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTVYVFLYGRLYLVLSGLE+ LS+  A +
Sbjct: 1454 LYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTVYVFLYGRLYLVLSGLEEGLSSQRAFR 1513

Query: 372  DNKSLQTALISQSAVQIGLLMALPMMVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGT 431
            +NK L+ AL SQS VQIG LMALPMM+EIGLERGF  AL +F+LMQLQLA VFFTF LGT
Sbjct: 1514 NNKPLEAALASQSFVQIGFLMALPMMMEIGLERGFHNALIEFVLMQLQLASVFFTFQLGT 1573

Query: 432  KTHYYGRTLLHGGAQYRGTGRGFVVFHAKFADNYRMYSRSHFVKGIELMILLIVYHILGV 491
            KTHYYGRTL HGGA+YRGTGRGFVVFHAKFA+NYR YSRSHFVKGIELMILL+VY I G 
Sbjct: 1574 KTHYYGRTLFHGGAEYRGTGRGFVVFHAKFAENYRFYSRSHFVKGIELMILLLVYQIFGQ 1633

Query: 492  SYRGVVAHVLITISIWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVSP 551
            SYRGVV ++LIT+SIWFMV TWLFAPFLFNPSGFEWQKIVDDWTDW KWI NRGGIGV P
Sbjct: 1634 SYRGVVTYILITVSIWFMVVTWLFAPFLFNPSGFEWQKIVDDWTDWNKWIYNRGGIGVPP 1693

Query: 552  DKSWESWWEKEHEHLLYSGKRGIFVEILLSLRFFIFQYGLVYHLKII--DSQSFLVYGLS 611
            +KSWESWWEKE EHL +SG RGI +EI L+LRFFIFQYGLVYHL      +QSF VYG S
Sbjct: 1694 EKSWESWWEKELEHLRHSGVRGITLEIFLALRFFIFQYGLVYHLSTFKGKNQSFWVYGAS 1753

Query: 612  WVVIISILLLMKAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAVPHMTIRDIIL 671
            W VI+ ILL++K + VGRR+FS +FQLLFR+ +G +F+  VA  IT +A+P +TI+D+ +
Sbjct: 1754 WFVILFILLIVKGLGVGRRRFSTNFQLLFRIIKGLVFLTFVAILITFLALPLITIKDLFI 1813

Query: 672  CLLAFLPTGWGLLLIAQACKRLIHHTALWGSVRALARSYEILMGLLLFTPVAFLAWFPFV 731
            C+LAF+PTGWG+LLIAQACK LI    +W SVR LAR YEI+MGLLLFTPVAFLAWFPFV
Sbjct: 1814 CMLAFMPTGWGMLLIAQACKPLIQQLGIWSSVRTLARGYEIVMGLLLFTPVAFLAWFPFV 1873

Query: 732  SEFQTRMLFNQAFSRGLQISRILGGPRKKEKSSSTD 766
            SEFQTRMLFNQAFSRGLQISRILGG RK   S + +
Sbjct: 1874 SEFQTRMLFNQAFSRGLQISRILGGQRKDRSSKNKE 1909

BLAST of Spg013975 vs. TAIR 10
Match: AT2G31960.1 (glucan synthase-like 3 )

HSP 1 Score: 779.2 bits (2011), Expect = 2.9e-225
Identity = 395/516 (76.55%), Postives = 439/516 (85.08%), Query Frame = 0

Query: 252  RIFNDTIGSFDGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD 311
            ++ N +   F GFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD
Sbjct: 1435 KVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD 1494

Query: 312  IYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTVYVFLYGRLYLVLSGLEKQLSTHPAIK 371
            +YRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTVYVFLYGRLYLVLSGLE+ LS   A +
Sbjct: 1495 LYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTVYVFLYGRLYLVLSGLEEGLSNQKAFR 1554

Query: 372  DNKSLQTALISQSAVQIGLLMALPMMVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGT 431
             N  LQ AL SQS VQIG LMALPMM+EIGLERGF  AL DF+LMQLQLA VFFTF LGT
Sbjct: 1555 SNMPLQAALASQSFVQIGFLMALPMMMEIGLERGFHNALIDFVLMQLQLASVFFTFQLGT 1614

Query: 432  KTHYYGRTLLHGGAQYRGTGRGFVVFHAKFADNYRMYSRSHFVKGIELMILLIVYHILGV 491
            KTHYYGRTL HGGA+YRGTGRGFVVFHAKFA+NYR YSRSHFVKGIELMILL+VY I G 
Sbjct: 1615 KTHYYGRTLFHGGAEYRGTGRGFVVFHAKFAENYRFYSRSHFVKGIELMILLLVYQIFGH 1674

Query: 492  SYRGVVAHVLITISIWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVSP 551
            +YRGVV ++LIT+SIWFMV TWLFAPFLFNPSGFEWQKIVDDWTDW KWI NRGGIGV P
Sbjct: 1675 AYRGVVTYILITVSIWFMVVTWLFAPFLFNPSGFEWQKIVDDWTDWNKWIYNRGGIGVPP 1734

Query: 552  DKSWESWWEKEHEHLLYSGKRGIFVEILLSLRFFIFQYGLVYHLKII--DSQSFLVYGLS 611
            +KSWESWWEKE  HL +SGKRGI +EI+L+LRFFIFQYGLVY L     ++QS  +YG S
Sbjct: 1735 EKSWESWWEKEIGHLRHSGKRGIILEIVLALRFFIFQYGLVYQLSTFKQENQSLWIYGAS 1794

Query: 612  WVVIISILLLMKAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAVPHMTIRDIIL 671
            W VI+ ILL++K + VGR++FS +FQLLFR+ +GF+F+  +   IT +A+  +T +DI L
Sbjct: 1795 WFVILFILLIVKGLGVGRQRFSTNFQLLFRIIKGFVFLTFLGLLITFLALRFLTPKDIFL 1854

Query: 672  CLLAFLPTGWGLLLIAQACKRLIHHTALWGSVRALARSYEILMGLLLFTPVAFLAWFPFV 731
            C+LAF+PTGWG+LLIAQACK LI     W SVR LAR YEILMGLLLFTPVAFLAWFPFV
Sbjct: 1855 CMLAFMPTGWGMLLIAQACKPLIQRLGFWSSVRTLARGYEILMGLLLFTPVAFLAWFPFV 1914

Query: 732  SEFQTRMLFNQAFSRGLQISRILGGPRKKEKSSSTD 766
            SEFQTRMLFNQAFSRGLQISRILGG RK   S + +
Sbjct: 1915 SEFQTRMLFNQAFSRGLQISRILGGQRKDRSSKNKE 1950

BLAST of Spg013975 vs. TAIR 10
Match: AT2G31960.2 (glucan synthase-like 3 )

HSP 1 Score: 779.2 bits (2011), Expect = 2.9e-225
Identity = 395/516 (76.55%), Postives = 439/516 (85.08%), Query Frame = 0

Query: 252  RIFNDTIGSFDGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD 311
            ++ N +   F GFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD
Sbjct: 1435 KVINLSEDIFAGFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRD 1494

Query: 312  IYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTVYVFLYGRLYLVLSGLEKQLSTHPAIK 371
            +YRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTVYVFLYGRLYLVLSGLE+ LS   A +
Sbjct: 1495 LYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTVYVFLYGRLYLVLSGLEEGLSNQKAFR 1554

Query: 372  DNKSLQTALISQSAVQIGLLMALPMMVEIGLERGFRAALSDFILMQLQLAPVFFTFSLGT 431
             N  LQ AL SQS VQIG LMALPMM+EIGLERGF  AL DF+LMQLQLA VFFTF LGT
Sbjct: 1555 SNMPLQAALASQSFVQIGFLMALPMMMEIGLERGFHNALIDFVLMQLQLASVFFTFQLGT 1614

Query: 432  KTHYYGRTLLHGGAQYRGTGRGFVVFHAKFADNYRMYSRSHFVKGIELMILLIVYHILGV 491
            KTHYYGRTL HGGA+YRGTGRGFVVFHAKFA+NYR YSRSHFVKGIELMILL+VY I G 
Sbjct: 1615 KTHYYGRTLFHGGAEYRGTGRGFVVFHAKFAENYRFYSRSHFVKGIELMILLLVYQIFGH 1674

Query: 492  SYRGVVAHVLITISIWFMVGTWLFAPFLFNPSGFEWQKIVDDWTDWKKWIVNRGGIGVSP 551
            +YRGVV ++LIT+SIWFMV TWLFAPFLFNPSGFEWQKIVDDWTDW KWI NRGGIGV P
Sbjct: 1675 AYRGVVTYILITVSIWFMVVTWLFAPFLFNPSGFEWQKIVDDWTDWNKWIYNRGGIGVPP 1734

Query: 552  DKSWESWWEKEHEHLLYSGKRGIFVEILLSLRFFIFQYGLVYHLKII--DSQSFLVYGLS 611
            +KSWESWWEKE  HL +SGKRGI +EI+L+LRFFIFQYGLVY L     ++QS  +YG S
Sbjct: 1735 EKSWESWWEKEIGHLRHSGKRGIILEIVLALRFFIFQYGLVYQLSTFKQENQSLWIYGAS 1794

Query: 612  WVVIISILLLMKAVSVGRRKFSASFQLLFRLAEGFIFIICVAGFITLVAVPHMTIRDIIL 671
            W VI+ ILL++K + VGR++FS +FQLLFR+ +GF+F+  +   IT +A+  +T +DI L
Sbjct: 1795 WFVILFILLIVKGLGVGRQRFSTNFQLLFRIIKGFVFLTFLGLLITFLALRFLTPKDIFL 1854

Query: 672  CLLAFLPTGWGLLLIAQACKRLIHHTALWGSVRALARSYEILMGLLLFTPVAFLAWFPFV 731
            C+LAF+PTGWG+LLIAQACK LI     W SVR LAR YEILMGLLLFTPVAFLAWFPFV
Sbjct: 1855 CMLAFMPTGWGMLLIAQACKPLIQRLGFWSSVRTLARGYEILMGLLLFTPVAFLAWFPFV 1914

Query: 732  SEFQTRMLFNQAFSRGLQISRILGGPRKKEKSSSTD 766
            SEFQTRMLFNQAFSRGLQISRILGG RK   S + +
Sbjct: 1915 SEFQTRMLFNQAFSRGLQISRILGGQRKDRSSKNKE 1950

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038898164.12.0e-27995.93callose synthase 1-like isoform X2 [Benincasa hispida][more]
XP_038898161.12.0e-27995.93callose synthase 1-like isoform X1 [Benincasa hispida] >XP_038898162.1 callose s... [more]
XP_022953403.14.1e-27795.16callose synthase 1 [Cucurbita moschata] >XP_022953404.1 callose synthase 1 [Cucu... [more]
KAG6575662.14.1e-27795.16Callose synthase 1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022991842.14.1e-27795.16callose synthase 1 [Cucurbita maxima] >XP_022991843.1 callose synthase 1 [Cucurb... [more]
Match NameE-valueIdentityDescription
Q9LXT92.9e-23378.25Callose synthase 3 OS=Arabidopsis thaliana OX=3702 GN=CALS3 PE=3 SV=3[more]
Q9AUE04.0e-22777.33Callose synthase 1 OS=Arabidopsis thaliana OX=3702 GN=CALS1 PE=1 SV=2[more]
Q9SL034.2e-22476.55Callose synthase 2 OS=Arabidopsis thaliana OX=3702 GN=CALS2 PE=2 SV=3[more]
Q3B7241.4e-19267.33Callose synthase 5 OS=Arabidopsis thaliana OX=3702 GN=CALS5 PE=1 SV=1[more]
Q9SHJ31.2e-19164.13Callose synthase 7 OS=Arabidopsis thaliana OX=3702 GN=CALS7 PE=3 SV=3[more]
Match NameE-valueIdentityDescription
A0A6J1JU282.0e-27795.161,3-beta-glucan synthase OS=Cucurbita maxima OX=3661 GN=LOC111488369 PE=3 SV=1[more]
A0A6J1GN912.0e-27795.161,3-beta-glucan synthase OS=Cucurbita moschata OX=3662 GN=LOC111455968 PE=3 SV=1[more]
A0A0A0K8F72.2e-27694.381,3-beta-glucan synthase OS=Cucumis sativus OX=3659 GN=Csa_7G429550 PE=3 SV=1[more]
A0A5A7UU131.1e-27596.431,3-beta-glucan synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A6J1CXR82.3e-27393.221,3-beta-glucan synthase OS=Momordica charantia OX=3673 GN=LOC111015422 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT5G13000.12.0e-23478.25glucan synthase-like 12 [more]
AT1G05570.12.8e-22877.33callose synthase 1 [more]
AT1G05570.22.8e-22877.33callose synthase 1 [more]
AT2G31960.12.9e-22576.55glucan synthase-like 3 [more]
AT2G31960.22.9e-22576.55glucan synthase-like 3 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003440Glycosyl transferase, family 48PFAMPF02364Glucan_synthasecoord: 261..572
e-value: 7.3E-98
score: 328.8
IPR026960Reverse transcriptase zinc-binding domainPFAMPF13966zf-RVTcoord: 107..196
e-value: 8.4E-13
score: 48.8
NoneNo IPR availablePANTHERPTHR12741LYST-INTERACTING PROTEIN LIP5 DOPAMINE RESPONSIVE PROTEIN DRG-1coord: 253..760
IPR026953Callose synthasePANTHERPTHR12741:SF70CALLOSE SYNTHASE 2coord: 253..760

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg013975.1Spg013975.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006075 (1->3)-beta-D-glucan biosynthetic process
biological_process GO:0071555 cell wall organization
biological_process GO:0008360 regulation of cell shape
cellular_component GO:0000148 1,3-beta-D-glucan synthase complex
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0003843 1,3-beta-D-glucan synthase activity