Spg013960 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg013960
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionAdenine DNA glycosylase
Locationscaffold3: 41365399 .. 41368404 (+)
RNA-Seq ExpressionSpg013960
SyntenySpg013960
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCCCAAGCTGCTCAACATGCCCTGTATTTGATCACTGCAAGGCCCTTTCGATCTCAAAGCATGATAGTTCAGTTCTTGTCACAGATTATCCCGCTAAGGGGATAAAGACCAAACAAAGACATGATTATTCTGCTGTATGTGTGGTTGAGATATTGGAAAATCAGGGCACATCTGAGTTAGAGCAATCTAGTAGATTTCTTCTTGTAAAGAGACCCGATGAAGGTTTGCTTGCTGGTCTATGGGAATTCCCATCTGTTTTGTTGGATGGAGAAGCTGATTTAAGTACAAGGAGAGAATCCATTAATAACCTCTTGAGTCAATGCTTTGGACTTGAACCAAAAAAGAATTTTGAAATAGTTATTAGAGAAGATGTTGGAGATTTTGTCCATGTTTTCACACACATCCGTCTCAAGATATATGTTGAACACTTGGTGTTATGTTTAAAAGGTTCGAGTTACTTTCTCCTTTCATCCCTATCTGTGCATTGAATATACATAAACTCAGTGACTAGAAACTTCCGACAAAACTTAACCCATATGAGATTTAATTATCTTATAGGCTGTCAAACATCGTATGTATGTTAAATATTTATTTCTTCCATGTCGTCTTTCACCGATGATTTATTTTCAATTGTCATAGATGGAAAACTATCACTGAAACTGGTCTATGAGGCAAACCATCAAATAAAGTTCAGACTTTTGAAACATTACTCTGTATAATTTTAAGCTTTCAAATTCCAATTATCATTTTAGTTTAGGATTCAGTTAGTATTTTGAATTTCTCATTGACTGCCCTCTCAGATTTGTTTTGTACTGCTTCTTTATTAATGCCTATTGTGCTAATTTTTCTGTGGGCTCCCTTGGCTTCAATGGGATATCTTATACTTTTCTCCCTTCGCAATTTTATTTTTTCCAATGAAGTTTCTGTTCCCTATACACACATAAAATAGGAATAAATAACCTCTTAAATTTACATTCTATTCACCATCGAAATAAATTCTTATTCTGTTTATGTAAGTGTTTGAAACTTCATGGCTCTGAACCATCGCTAGTGTAACAAAGGAGCACTTGATGGTTTATTGTTCATAGAATTGCGGTTACACTGTGTTTAAATTGCCTGAAACAAAACATCACTTAGTCCTTAGTATTCGGCTGCAACACCTATACTCAATCTGGAGTCAGACAAGGGTATGAAGTGATTTTCATTTTTCCTACTAATATTTTATCTTTCCTTGACTGTTGCATTTGCGACATGTAATATCTTATTGTTAAATACACTTGAATTTTTTAATAAATGGAATAAGTCATTGTGATTTAGTATGGCAAACCATGTTGATCTGAATTATAATTTACATTATAAATTGATTTCAACGGCATTTATTAGGTGTAAAATGATTCTATTTTCATATTTATAGTAAATGGTATTGTTGATGTCTAAAATTTTATTCTAGCACAATAAATTGCTCTACTGCCGAGCAGGTGTCTAGTTATTTATCAATCTTTTTAATTTTTACTGTCGTTGATTCTTAGTAATTAGTCATCCTCGTAGATCATTTTGAAGCATTTCAAGCTGATAATAATGAAAGCTGCTTACATTTACTTGGAATTGGTTTTCATCAGTTTTCCTGAATCTTATGCTGTAGATAGGCGTGTTTTCTGTGACTATGCGAAGTACTTTGCTCATCAAAGGCCATGGGACATGACTATGGAAAAAACAGTTCTTTTTGGAAATTTGTGAATCATAGTCCTGTTTTTCCATTTCAGTAAATTTACTTCACATATTTATATGTTCTTAAAGGTGAAGGTAGCAAGTTGTTCCGGAGACGGGAGAAGAAATCTACATTATGGAAATGTGTGGACAACAAGGTTATGTCAACCATGGGATTGACGTCCAGTGTGAGGAAGGTAAGCACAGATGGTGCATTAGATGACTTCTCATTGTGTTCCTTTATTTTTACTTTCATAAATATCTAATTCAACGGTTTTATTTTCATCTACTTAAAATCTTAATCTATCTATACAATCCGGCATCGTTAAATTTGGAAGAAAGTTCTTTCAGATTTCTTAGTACCAAAATTTATGTACCAACCCAGATTCGATGCAGAGTAAACAGAGATTTGATATTTGTAACTGCAAGTTTTGATGAGAGTCGAGCATAATATGAAATATCTTCTTGCTGTCCCATGGGGACATAATTATAAACCGTATGATTCTAGTAAATGCTGGTACCAGAACTATATACTGTCACTTTTTTTTCCCTTTTTTCTTGGGCACGTCGTTGATTGGGATAAGAACAGATACTACACTTGATCTTAGCCAAAAGTCCGAGAAAGGTATTAGAGTTTAGGGTTATACTGGATGTTTGAGCTTCAATTAGCAACATCTAGCAGATTATAAAATCTGAATATCAACTTTATTCAAGGAAAATTTAAATTTCCTTGAACATCCATATCAGTTTAGTTATCGAATGTGGGTTGTTCATAAGAGAGAATTGTTTTTTTATCATGGAAATGTGTGATAAGACAATGAGTTATTAGCAACTAATTTGACAACTTTCTGACAGGCCTATGCCATGGTCGAGAAATTTCAGGCAGAGAAGACATGTTCTAGCCGTGCACTAGCCCGAAAAAGACAGAGAGTTACTTCAACAAGCTTGAAGTAAAATAGAAGGGAAAATTCGTGGGGAGAGTTATGGTTTGATTTCTCCTTGATAGATAAGAGTTGGATAAATAGATACTGTTGAGTAGCAAAATGTTCAAGAATGTATCCACTCAGAATTCGTACACCAAAATATTTGACTGCACCGGAATTTGGAGGAATGTACTCAAGCCAGTGGCTATTCAAGGCGCACATGGAATTTACTCTTGTTGAAGGGTGGCTGTTGATGGATGTGGTGATGCTCTAAGAGAACATGGAGCTTCATCCAATATTGTTTTAAACATGGAGTTTGATTCCAATGAGCAAATGGAAGCTTCAGTGGATTTTCTGAACTTTATCATTTGA

mRNA sequence

ATGAGCCCAAGCTGCTCAACATGCCCTGTATTTGATCACTGCAAGGCCCTTTCGATCTCAAAGCATGATAGTTCAGTTCTTGTCACAGATTATCCCGCTAAGGGGATAAAGACCAAACAAAGACATGATTATTCTGCTGTATGTGTGGTTGAGATATTGGAAAATCAGGGCACATCTGAGTTAGAGCAATCTAGTAGATTTCTTCTTGTAAAGAGACCCGATGAAGGTTTGCTTGCTGGTCTATGGGAATTCCCATCTGTTTTGTTGGATGGAGAAGCTGATTTAAGTACAAGGAGAGAATCCATTAATAACCTCTTGAGTCAATGCTTTGGACTTGAACCAAAAAAGAATTTTGAAATAGTTATTAGAGAAGATGTTGGAGATTTTGTCCATGTTTTCACACACATCCGTCTCAAGATATATGTTGAACACTTGGTGTTATGTTTAAAAGGCGTGTTTTCTGTGACTATGCGAAGTGAAGGTAGCAAGTTGTTCCGGAGACGGGAGAAGAAATCTACATTATGGAAATGTGTGGACAACAAGGTTATGTCAACCATGGGATTGACGTCCAGTGTGAGGAAGGCCTATGCCATGGTCGAGAAATTTCAGGCAGAGAAGACATGTTCTAGCCGTGCACTAGCCCGAAAAAGACAGAGAGTTACTTCAACAAGCTTGAAGGTGGCTGTTGATGGATGTGGTGATGCTCTAAGAGAACATGGAGCTTCATCCAATATTGTTTTAAACATGGAGTTTGATTCCAATGAGCAAATGGAAGCTTCAGTGGATTTTCTGAACTTTATCATTTGA

Coding sequence (CDS)

ATGAGCCCAAGCTGCTCAACATGCCCTGTATTTGATCACTGCAAGGCCCTTTCGATCTCAAAGCATGATAGTTCAGTTCTTGTCACAGATTATCCCGCTAAGGGGATAAAGACCAAACAAAGACATGATTATTCTGCTGTATGTGTGGTTGAGATATTGGAAAATCAGGGCACATCTGAGTTAGAGCAATCTAGTAGATTTCTTCTTGTAAAGAGACCCGATGAAGGTTTGCTTGCTGGTCTATGGGAATTCCCATCTGTTTTGTTGGATGGAGAAGCTGATTTAAGTACAAGGAGAGAATCCATTAATAACCTCTTGAGTCAATGCTTTGGACTTGAACCAAAAAAGAATTTTGAAATAGTTATTAGAGAAGATGTTGGAGATTTTGTCCATGTTTTCACACACATCCGTCTCAAGATATATGTTGAACACTTGGTGTTATGTTTAAAAGGCGTGTTTTCTGTGACTATGCGAAGTGAAGGTAGCAAGTTGTTCCGGAGACGGGAGAAGAAATCTACATTATGGAAATGTGTGGACAACAAGGTTATGTCAACCATGGGATTGACGTCCAGTGTGAGGAAGGCCTATGCCATGGTCGAGAAATTTCAGGCAGAGAAGACATGTTCTAGCCGTGCACTAGCCCGAAAAAGACAGAGAGTTACTTCAACAAGCTTGAAGGTGGCTGTTGATGGATGTGGTGATGCTCTAAGAGAACATGGAGCTTCATCCAATATTGTTTTAAACATGGAGTTTGATTCCAATGAGCAAATGGAAGCTTCAGTGGATTTTCTGAACTTTATCATTTGA

Protein sequence

MSPSCSTCPVFDHCKALSISKHDSSVLVTDYPAKGIKTKQRHDYSAVCVVEILENQGTSELEQSSRFLLVKRPDEGLLAGLWEFPSVLLDGEADLSTRRESINNLLSQCFGLEPKKNFEIVIREDVGDFVHVFTHIRLKIYVEHLVLCLKGVFSVTMRSEGSKLFRRREKKSTLWKCVDNKVMSTMGLTSSVRKAYAMVEKFQAEKTCSSRALARKRQRVTSTSLKVAVDGCGDALREHGASSNIVLNMEFDSNEQMEASVDFLNFII
Homology
BLAST of Spg013960 vs. NCBI nr
Match: XP_038874808.1 (adenine DNA glycosylase isoform X1 [Benincasa hispida])

HSP 1 Score: 365.5 bits (937), Expect = 3.8e-97
Identity = 185/218 (84.86%), Postives = 202/218 (92.66%), Query Frame = 0

Query: 2   SPSCSTCPVFDHCKALSISKHDSSVLVTDYPAKGIKTKQRHDYSAVCVVEILENQGTSEL 61
           +PSCSTCPVFDHC+ALSISKHD+S+LVTDYPAKGIKTKQRHDYSAVCVVEIL+NQGTSEL
Sbjct: 292 NPSCSTCPVFDHCEALSISKHDNSILVTDYPAKGIKTKQRHDYSAVCVVEILDNQGTSEL 351

Query: 62  EQSSRFLLVKRPDEGLLAGLWEFPSVLLDGEADLSTRRESINNLLSQCFGLEPKKNFEIV 121
           EQSSRFLLVKRPDEGLLAGLWEFPSVLLDGEADL+TRRESIN+ LS+CFGLE KKNFEIV
Sbjct: 352 EQSSRFLLVKRPDEGLLAGLWEFPSVLLDGEADLNTRRESINSHLSKCFGLELKKNFEIV 411

Query: 122 IREDVGDFVHVFTHIRLKIYVEHLVLCLKGVFSVTMRSEGSKLFRRREKKSTLWKCVDNK 181
           IREDVGDF+HVFTHIRLKIYVEHLVLCLKG        EGS+LF ++EKKS LW+CVDNK
Sbjct: 412 IREDVGDFIHVFTHIRLKIYVEHLVLCLKG--------EGSELFLKQEKKSILWRCVDNK 471

Query: 182 VMSTMGLTSSVRKAYAMVEKFQAEKTCSSRALARKRQR 220
            MS+MGLTSSVRK YAMVEKFQA+KT SSRAL RK+Q+
Sbjct: 472 AMSSMGLTSSVRKTYAMVEKFQAKKT-SSRALPRKKQK 500

BLAST of Spg013960 vs. NCBI nr
Match: XP_038874810.1 (adenine DNA glycosylase isoform X3 [Benincasa hispida])

HSP 1 Score: 365.5 bits (937), Expect = 3.8e-97
Identity = 185/218 (84.86%), Postives = 202/218 (92.66%), Query Frame = 0

Query: 2   SPSCSTCPVFDHCKALSISKHDSSVLVTDYPAKGIKTKQRHDYSAVCVVEILENQGTSEL 61
           +PSCSTCPVFDHC+ALSISKHD+S+LVTDYPAKGIKTKQRHDYSAVCVVEIL+NQGTSEL
Sbjct: 252 NPSCSTCPVFDHCEALSISKHDNSILVTDYPAKGIKTKQRHDYSAVCVVEILDNQGTSEL 311

Query: 62  EQSSRFLLVKRPDEGLLAGLWEFPSVLLDGEADLSTRRESINNLLSQCFGLEPKKNFEIV 121
           EQSSRFLLVKRPDEGLLAGLWEFPSVLLDGEADL+TRRESIN+ LS+CFGLE KKNFEIV
Sbjct: 312 EQSSRFLLVKRPDEGLLAGLWEFPSVLLDGEADLNTRRESINSHLSKCFGLELKKNFEIV 371

Query: 122 IREDVGDFVHVFTHIRLKIYVEHLVLCLKGVFSVTMRSEGSKLFRRREKKSTLWKCVDNK 181
           IREDVGDF+HVFTHIRLKIYVEHLVLCLKG        EGS+LF ++EKKS LW+CVDNK
Sbjct: 372 IREDVGDFIHVFTHIRLKIYVEHLVLCLKG--------EGSELFLKQEKKSILWRCVDNK 431

Query: 182 VMSTMGLTSSVRKAYAMVEKFQAEKTCSSRALARKRQR 220
            MS+MGLTSSVRK YAMVEKFQA+KT SSRAL RK+Q+
Sbjct: 432 AMSSMGLTSSVRKTYAMVEKFQAKKT-SSRALPRKKQK 460

BLAST of Spg013960 vs. NCBI nr
Match: XP_038874809.1 (adenine DNA glycosylase isoform X2 [Benincasa hispida])

HSP 1 Score: 365.5 bits (937), Expect = 3.8e-97
Identity = 185/218 (84.86%), Postives = 202/218 (92.66%), Query Frame = 0

Query: 2   SPSCSTCPVFDHCKALSISKHDSSVLVTDYPAKGIKTKQRHDYSAVCVVEILENQGTSEL 61
           +PSCSTCPVFDHC+ALSISKHD+S+LVTDYPAKGIKTKQRHDYSAVCVVEIL+NQGTSEL
Sbjct: 281 NPSCSTCPVFDHCEALSISKHDNSILVTDYPAKGIKTKQRHDYSAVCVVEILDNQGTSEL 340

Query: 62  EQSSRFLLVKRPDEGLLAGLWEFPSVLLDGEADLSTRRESINNLLSQCFGLEPKKNFEIV 121
           EQSSRFLLVKRPDEGLLAGLWEFPSVLLDGEADL+TRRESIN+ LS+CFGLE KKNFEIV
Sbjct: 341 EQSSRFLLVKRPDEGLLAGLWEFPSVLLDGEADLNTRRESINSHLSKCFGLELKKNFEIV 400

Query: 122 IREDVGDFVHVFTHIRLKIYVEHLVLCLKGVFSVTMRSEGSKLFRRREKKSTLWKCVDNK 181
           IREDVGDF+HVFTHIRLKIYVEHLVLCLKG        EGS+LF ++EKKS LW+CVDNK
Sbjct: 401 IREDVGDFIHVFTHIRLKIYVEHLVLCLKG--------EGSELFLKQEKKSILWRCVDNK 460

Query: 182 VMSTMGLTSSVRKAYAMVEKFQAEKTCSSRALARKRQR 220
            MS+MGLTSSVRK YAMVEKFQA+KT SSRAL RK+Q+
Sbjct: 461 AMSSMGLTSSVRKTYAMVEKFQAKKT-SSRALPRKKQK 489

BLAST of Spg013960 vs. NCBI nr
Match: XP_022953220.1 (adenine DNA glycosylase [Cucurbita moschata])

HSP 1 Score: 364.0 bits (933), Expect = 1.1e-96
Identity = 188/224 (83.93%), Postives = 202/224 (90.18%), Query Frame = 0

Query: 2   SPSCSTCPVFDHCKALSISKHDSSVLVTDYPAKGIKTKQRHDYSAVCVVEILENQGTSEL 61
           SPSCSTCPVFDHC+ALS SK DSSVLVTDYPAKG+KTKQRHDYSAVCVVEILENQGTSEL
Sbjct: 244 SPSCSTCPVFDHCEALSSSKDDSSVLVTDYPAKGVKTKQRHDYSAVCVVEILENQGTSEL 303

Query: 62  EQSSRFLLVKRPDEGLLAGLWEFPSVLLDGEADLSTRRESINNLLSQCFGLEPKKNFEIV 121
           +QSSRFLLVKRPDEGLLAGLWEFPSVLL+GEAD STRRESIN+LLS+ FGLEPKKNFEIV
Sbjct: 304 KQSSRFLLVKRPDEGLLAGLWEFPSVLLNGEADSSTRRESINSLLSKSFGLEPKKNFEIV 363

Query: 122 IREDVGDFVHVFTHIRLKIYVEHLVLCLKGVFSVTMRSEGSKLFRRREKKSTLWKCVDNK 181
           IREDVGDFVHVF+HIRLKIYVEHLVL LKG        EGSKLFR++EKKS  WKCVDNK
Sbjct: 364 IREDVGDFVHVFSHIRLKIYVEHLVLRLKG--------EGSKLFRKQEKKSISWKCVDNK 423

Query: 182 VMSTMGLTSSVRKAYAMVEKFQAEKTCSSRALARKRQRVTSTSL 226
           VMS+MGLTSSVRK YAMVEKF+AEK   S A+A K+QR TST+L
Sbjct: 424 VMSSMGLTSSVRKVYAMVEKFEAEKISPSPAVATKKQRPTSTNL 459

BLAST of Spg013960 vs. NCBI nr
Match: KAG6576094.1 (Adenine DNA glycosylase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 361.3 bits (926), Expect = 7.2e-96
Identity = 187/224 (83.48%), Postives = 201/224 (89.73%), Query Frame = 0

Query: 2   SPSCSTCPVFDHCKALSISKHDSSVLVTDYPAKGIKTKQRHDYSAVCVVEILENQGTSEL 61
           SPSCSTCPVFDHC+ALSISK DSSVLVTDYPAKGIKTKQRHDYSAVCVVEILENQ TSEL
Sbjct: 244 SPSCSTCPVFDHCEALSISKDDSSVLVTDYPAKGIKTKQRHDYSAVCVVEILENQSTSEL 303

Query: 62  EQSSRFLLVKRPDEGLLAGLWEFPSVLLDGEADLSTRRESINNLLSQCFGLEPKKNFEIV 121
           +QSSRFLLVKRPDEGLLAGLWEFPSVLL+GEAD STRRESIN+LLS+ FGLEPKKNFEIV
Sbjct: 304 KQSSRFLLVKRPDEGLLAGLWEFPSVLLNGEADSSTRRESINSLLSKSFGLEPKKNFEIV 363

Query: 122 IREDVGDFVHVFTHIRLKIYVEHLVLCLKGVFSVTMRSEGSKLFRRREKKSTLWKCVDNK 181
           IREDVGDFVHVF+HIRLKIYVEHLV+ LKG        EGSKLFR++EKKS  WKCVD K
Sbjct: 364 IREDVGDFVHVFSHIRLKIYVEHLVIRLKG--------EGSKLFRKQEKKSISWKCVDKK 423

Query: 182 VMSTMGLTSSVRKAYAMVEKFQAEKTCSSRALARKRQRVTSTSL 226
           VMS+MGLTSSVRK YAMVEKF+AEK   S A+A K+QR TST+L
Sbjct: 424 VMSSMGLTSSVRKVYAMVEKFEAEKISPSPAVATKKQRPTSTNL 459

BLAST of Spg013960 vs. ExPASy Swiss-Prot
Match: F4JRF4 (Adenine DNA glycosylase OS=Arabidopsis thaliana OX=3702 GN=MYH PE=3 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 1.9e-46
Identity = 99/194 (51.03%), Postives = 131/194 (67.53%), Query Frame = 0

Query: 3   PSCSTCPVFDHCKALSISKHDSSVLVTDYPAKGIKTKQRHDYSAVCVVEILENQGTSELE 62
           PSCS+CPV   C+A S+S+ + ++ VTDYP K IK K RHD+  VCV+EI         +
Sbjct: 346 PSCSSCPVSSQCRAFSLSEENRTISVTDYPTKVIKAKPRHDFCCVCVLEI---HNLERNQ 405

Query: 63  QSSRFLLVKRPDEGLLAGLWEFPSVLLDGEADLSTRRESINNLLSQC--FGLEPKKNFEI 122
              RF+LVKRP++GLLAGLWEFPSV+L+ EAD +TRR +IN  L +   F +E KK   I
Sbjct: 406 SGGRFVLVKRPEQGLLAGLWEFPSVILNEEADSATRRNAINVYLKEAFRFHVELKKACTI 465

Query: 123 VIREDVGDFVHVFTHIRLKIYVEHLVLCLKGVFSVTMRSEGSKLFRRREKKSTLWKCVDN 182
           V RE++G+FVH+FTHIR K+YVE LV+ L G            LF+ + K +  WKCV +
Sbjct: 466 VSREELGEFVHIFTHIRRKVYVELLVVQLTG--------GTEDLFKGQAKDTLTWKCVSS 525

Query: 183 KVMSTMGLTSSVRK 195
            V+ST+GLTS+VRK
Sbjct: 526 DVLSTLGLTSAVRK 528

BLAST of Spg013960 vs. ExPASy Swiss-Prot
Match: Q99P21 (Adenine DNA glycosylase OS=Mus musculus OX=10090 GN=Mutyh PE=1 SV=2)

HSP 1 Score: 69.7 bits (169), Expect = 5.7e-11
Identity = 55/177 (31.07%), Postives = 75/177 (42.37%), Query Frame = 0

Query: 3   PSCSTCPVFDHCKA--------------------------------LSISKHDSSVLVTD 62
           P CS CPV   C+A                                 S S  D S+ V +
Sbjct: 266 PLCSHCPVQSLCRAYQRVQRGQLSALPGRPDIEECALNTRQCQLCLTSSSPWDPSMGVAN 325

Query: 63  YPAKGIKTKQRHDYSAVCVVEILENQGTSELEQSSRFLLVKRPDEGLLAGLWEFPSVLLD 122
           +P K  +   R +YSA CVVE     G          LLV+RPD GLLAGLWEFPSV L+
Sbjct: 326 FPRKASRRPPREEYSATCVVEQPGAIG------GPLVLLVQRPDSGLLAGLWEFPSVTLE 385

Query: 123 GEADLSTRRESINNLLSQCFGLEPKKNFEIVIREDVGDFVHVFTHIRLKIYVEHLVL 148
             ++    +  +  L   C  L        +  + +G+ +H+F+HI+L   V  L L
Sbjct: 386 -PSEQHQHKALLQELQRWCGPL------PAIRLQHLGEVIHIFSHIKLTYQVYSLAL 429

BLAST of Spg013960 vs. ExPASy Swiss-Prot
Match: Q9UIF7 (Adenine DNA glycosylase OS=Homo sapiens OX=9606 GN=MUTYH PE=1 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 1.4e-09
Identity = 59/200 (29.50%), Postives = 95/200 (47.50%), Query Frame = 0

Query: 23  DSSVLVTDYPAKGIKTKQRHDYSAVCVVEILENQGTSELEQSSRFLLVKRPDEGLLAGLW 82
           D ++ V ++P K  +   R + SA CV   LE  G       ++ LLV+RP+ GLLAGLW
Sbjct: 347 DQTLGVVNFPRKASRKPPREESSATCV---LEQPGA----LGAQILLVQRPNSGLLAGLW 406

Query: 83  EFPSVLLDGEADLSTRRESINNLLSQCFGLEPKKNFEIVIREDVGDFVHVFTHIRLKIYV 142
           EFPSV  +    L  +R+++   L +  G  P  +        +G+ VH F+HI+L   V
Sbjct: 407 EFPSVTWEPSEQL--QRKALLQELQRWAGPLPATHL-----RHLGEVVHTFSHIKLTYQV 466

Query: 143 EHLVLCLKGVFSVTMRSEGSKLFRRREKKSTLWKCVDNKVMSTMGLTSSVRKAYAMVEKF 202
               L L+G   VT    G++   + E               T  ++++++K +  V + 
Sbjct: 467 --YGLALEGQTPVTTVPPGARWLTQEE-------------FHTAAVSTAMKKVF-RVYQG 512

Query: 203 QAEKTCSSRALARKRQRVTS 223
           Q   TC    +  KR +V+S
Sbjct: 527 QQPGTC----MGSKRSQVSS 512

BLAST of Spg013960 vs. ExPASy Swiss-Prot
Match: Q8R5G2 (Adenine DNA glycosylase OS=Rattus norvegicus OX=10116 GN=Mutyh PE=2 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 2.4e-09
Identity = 45/130 (34.62%), Postives = 64/130 (49.23%), Query Frame = 0

Query: 18  SISKHDSSVLVTDYPAKGIKTKQRHDYSAVCVVEILENQGTSELEQSSRFLLVKRPDEGL 77
           S +  D ++ V ++P K  +   R +YSA CVVE     G          LLV+RP+ GL
Sbjct: 313 STNPWDPNMGVVNFPRKASRRPPREEYSATCVVEQPGATG------GPLILLVQRPNSGL 372

Query: 78  LAGLWEFPSVLLDGEADLSTRRESINNLLSQCFGLEPKKNFEIVIREDVGDFVHVFTHIR 137
           LAGLWEFPSV L  E     + +++   L       P         + +G+ +HVF+HI+
Sbjct: 373 LAGLWEFPSVTL--EPSGQHQHKALLQELQHWSAPLPTTPL-----QHLGEVIHVFSHIK 429

Query: 138 LKIYVEHLVL 148
           L   V  L L
Sbjct: 433 LTYQVYSLAL 429

BLAST of Spg013960 vs. ExPASy Swiss-Prot
Match: Q10159 (Adenine DNA glycosylase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=myh1 PE=1 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.5e-08
Identity = 47/173 (27.17%), Postives = 75/173 (43.35%), Query Frame = 0

Query: 2   SPSCSTCPVFDHCKAL---------SISKHD-----SSVLVTD--------------YPA 61
           SP CS CP+ + CKA          +  K+D      ++ +TD              YP 
Sbjct: 230 SPRCSVCPISEICKAYQEQNVIRDGNTIKYDIEDVPCNICITDIPSKEDLQNWVVARYPV 289

Query: 62  KGIKTKQRHDYSAVCVVEILENQGTSELEQSSRFLLVKRPDEGLLAGLWEFPSVLLDGEA 121
              KTKQR + + V +      Q T    +   FL+ KRP  GLLAGLW+FP++    E+
Sbjct: 290 HPAKTKQREERALVVIF-----QKTDPSTKEKFFLIRKRPSAGLLAGLWDFPTIEFGQES 349

Query: 122 DLSTRRESINNLLSQCFGLEPKKNFEIVIREDVGDFVHVFTHIRLKIYVEHLV 147
                       ++Q    + +    I   +  G ++H+F+HIR   +V + +
Sbjct: 350 WPKDMDAEFQKSIAQWISNDSRS--LIKKYQSRGRYLHIFSHIRKTSHVFYAI 395

BLAST of Spg013960 vs. ExPASy TrEMBL
Match: A0A6J1GP17 (Adenine DNA glycosylase OS=Cucurbita moschata OX=3662 GN=LOC111455831 PE=3 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 5.4e-97
Identity = 188/224 (83.93%), Postives = 202/224 (90.18%), Query Frame = 0

Query: 2   SPSCSTCPVFDHCKALSISKHDSSVLVTDYPAKGIKTKQRHDYSAVCVVEILENQGTSEL 61
           SPSCSTCPVFDHC+ALS SK DSSVLVTDYPAKG+KTKQRHDYSAVCVVEILENQGTSEL
Sbjct: 244 SPSCSTCPVFDHCEALSSSKDDSSVLVTDYPAKGVKTKQRHDYSAVCVVEILENQGTSEL 303

Query: 62  EQSSRFLLVKRPDEGLLAGLWEFPSVLLDGEADLSTRRESINNLLSQCFGLEPKKNFEIV 121
           +QSSRFLLVKRPDEGLLAGLWEFPSVLL+GEAD STRRESIN+LLS+ FGLEPKKNFEIV
Sbjct: 304 KQSSRFLLVKRPDEGLLAGLWEFPSVLLNGEADSSTRRESINSLLSKSFGLEPKKNFEIV 363

Query: 122 IREDVGDFVHVFTHIRLKIYVEHLVLCLKGVFSVTMRSEGSKLFRRREKKSTLWKCVDNK 181
           IREDVGDFVHVF+HIRLKIYVEHLVL LKG        EGSKLFR++EKKS  WKCVDNK
Sbjct: 364 IREDVGDFVHVFSHIRLKIYVEHLVLRLKG--------EGSKLFRKQEKKSISWKCVDNK 423

Query: 182 VMSTMGLTSSVRKAYAMVEKFQAEKTCSSRALARKRQRVTSTSL 226
           VMS+MGLTSSVRK YAMVEKF+AEK   S A+A K+QR TST+L
Sbjct: 424 VMSSMGLTSSVRKVYAMVEKFEAEKISPSPAVATKKQRPTSTNL 459

BLAST of Spg013960 vs. ExPASy TrEMBL
Match: A0A6J1JU23 (Adenine DNA glycosylase OS=Cucurbita maxima OX=3661 GN=LOC111488366 PE=3 SV=1)

HSP 1 Score: 360.9 bits (925), Expect = 4.5e-96
Identity = 186/224 (83.04%), Postives = 201/224 (89.73%), Query Frame = 0

Query: 2   SPSCSTCPVFDHCKALSISKHDSSVLVTDYPAKGIKTKQRHDYSAVCVVEILENQGTSEL 61
           SPSCSTCPVFDHC+ALSISK DSSVLVTDYPAKGIKTKQRHDYSAVCVVE+LEN+GTSEL
Sbjct: 244 SPSCSTCPVFDHCEALSISKDDSSVLVTDYPAKGIKTKQRHDYSAVCVVEMLENRGTSEL 303

Query: 62  EQSSRFLLVKRPDEGLLAGLWEFPSVLLDGEADLSTRRESINNLLSQCFGLEPKKNFEIV 121
           +Q SRFLLVKRPDEGLLAGLWEFPSVLL+GEAD STRRESIN+LLS+ FGLEPKKNFEIV
Sbjct: 304 KQCSRFLLVKRPDEGLLAGLWEFPSVLLNGEADSSTRRESINSLLSKSFGLEPKKNFEIV 363

Query: 122 IREDVGDFVHVFTHIRLKIYVEHLVLCLKGVFSVTMRSEGSKLFRRREKKSTLWKCVDNK 181
           IREDVGDFVHVF+HIRLKIYVEHLVL LKG        EGSKLFR++EKKS  WKCVDNK
Sbjct: 364 IREDVGDFVHVFSHIRLKIYVEHLVLRLKG--------EGSKLFRKQEKKSISWKCVDNK 423

Query: 182 VMSTMGLTSSVRKAYAMVEKFQAEKTCSSRALARKRQRVTSTSL 226
           VMS+MGLTSSVRK Y MVEKF+AE    SRA+A K+QR TST+L
Sbjct: 424 VMSSMGLTSSVRKVYDMVEKFEAEMISPSRAVATKKQRATSTNL 459

BLAST of Spg013960 vs. ExPASy TrEMBL
Match: A0A5A7T8X3 (Adenine DNA glycosylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold122G001490 PE=3 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 5.9e-96
Identity = 187/220 (85.00%), Postives = 200/220 (90.91%), Query Frame = 0

Query: 2   SPSCSTCPVFDHCKALSISKHDSSVLVTDYPAKGIKTKQRHDYSAVCVVEILENQGTSEL 61
           +PSCSTCPVFDHC+ALSISK DSSVLVTDYPAKGIKTKQRHDYSAVCVVEILE+QGTSEL
Sbjct: 277 NPSCSTCPVFDHCEALSISKRDSSVLVTDYPAKGIKTKQRHDYSAVCVVEILESQGTSEL 336

Query: 62  EQSSRFLLVKRPDEGLLAGLWEFPSVLLDGEADLSTRRESINNLLSQCFGLEPKKNFEIV 121
            QSSRFLLVKRPDEGLLAGLWEFPSV LDGEADL+TRRESI++LLS+ FGLEPKKNFEIV
Sbjct: 337 GQSSRFLLVKRPDEGLLAGLWEFPSVSLDGEADLTTRRESIDSLLSKNFGLEPKKNFEIV 396

Query: 122 IREDVGDFVHVFTHIRLKIYVEHLVLCLKGVFSVTMRSEGSKLFRRREKKSTLWKCVDNK 181
            REDVGDF+HVFTHIRLKIYVEHLVLCLKG        EGSKLFR++EKKS LWKCV+NK
Sbjct: 397 NREDVGDFIHVFTHIRLKIYVEHLVLCLKG--------EGSKLFRKQEKKSILWKCVENK 456

Query: 182 VMSTMGLTSSVRKAYAMVEKFQAEKT--CSSRALARKRQR 220
           VMSTMGLTSSVRKAYAMVEKFQA KT   SSR L RK+Q+
Sbjct: 457 VMSTMGLTSSVRKAYAMVEKFQAGKTSSSSSRVLPRKKQK 488

BLAST of Spg013960 vs. ExPASy TrEMBL
Match: A0A0A0KC27 (Adenine DNA glycosylase OS=Cucumis sativus OX=3659 GN=Csa_6G088720 PE=3 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 2.9e-95
Identity = 186/220 (84.55%), Postives = 198/220 (90.00%), Query Frame = 0

Query: 2   SPSCSTCPVFDHCKALSISKHDSSVLVTDYPAKGIKTKQRHDYSAVCVVEILENQGTSEL 61
           +PSCSTCPVFDHC+ALSISKHDSSVLVTDYPAKGIK KQRHDYSAVCVVEILE+QGT EL
Sbjct: 306 NPSCSTCPVFDHCEALSISKHDSSVLVTDYPAKGIKIKQRHDYSAVCVVEILESQGTPEL 365

Query: 62  EQSSRFLLVKRPDEGLLAGLWEFPSVLLDGEADLSTRRESINNLLSQCFGLEPKKNFEIV 121
            QSSRFLLVKRPDEGLLAGLWEFPSV LDGEADLSTRRESIN+LLS+ FGLE KKNFEIV
Sbjct: 366 GQSSRFLLVKRPDEGLLAGLWEFPSVSLDGEADLSTRRESINSLLSKNFGLEAKKNFEIV 425

Query: 122 IREDVGDFVHVFTHIRLKIYVEHLVLCLKGVFSVTMRSEGSKLFRRREKKSTLWKCVDNK 181
            REDVGDF+H+FTHIRLKIYVEHLVLCLKG        EGSKLFR++EKKS LWKCV+NK
Sbjct: 426 NREDVGDFIHIFTHIRLKIYVEHLVLCLKG--------EGSKLFRKQEKKSILWKCVENK 485

Query: 182 VMSTMGLTSSVRKAYAMVEKFQAEKTCSSR--ALARKRQR 220
           VMSTMGLTSSVRKAYAMVEKFQA KT SS   AL RK+Q+
Sbjct: 486 VMSTMGLTSSVRKAYAMVEKFQAGKTSSSSNCALPRKKQK 517

BLAST of Spg013960 vs. ExPASy TrEMBL
Match: A0A1S3CBT2 (Adenine DNA glycosylase OS=Cucumis melo OX=3656 GN=LOC103498904 PE=3 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 1.1e-94
Identity = 186/220 (84.55%), Postives = 198/220 (90.00%), Query Frame = 0

Query: 2   SPSCSTCPVFDHCKALSISKHDSSVLVTDYPAKGIKTKQRHDYSAVCVVEILENQGTSEL 61
           +PSCSTCPVFDHC+ALSISK DSSVLVTDYPAKGIKTKQRHDYSAVCVVEILE+QGTSEL
Sbjct: 252 NPSCSTCPVFDHCEALSISKRDSSVLVTDYPAKGIKTKQRHDYSAVCVVEILESQGTSEL 311

Query: 62  EQSSRFLLVKRPDEGLLAGLWEFPSVLLDGEADLSTRRESINNLLSQCFGLEPKKNFEIV 121
            QSSRFLLVKRPDEGLLAGLWEFPSV LDGEAD STRRESI++LLS+ FGLEPKKNFEIV
Sbjct: 312 GQSSRFLLVKRPDEGLLAGLWEFPSVSLDGEADSSTRRESIDSLLSKNFGLEPKKNFEIV 371

Query: 122 IREDVGDFVHVFTHIRLKIYVEHLVLCLKGVFSVTMRSEGSKLFRRREKKSTLWKCVDNK 181
            REDVGDF+HVFTHIRLKIYVEHLVLCLKG        EGSKLFR++EKKS LWKCV+NK
Sbjct: 372 NREDVGDFIHVFTHIRLKIYVEHLVLCLKG--------EGSKLFRKQEKKSILWKCVENK 431

Query: 182 VMSTMGLTSSVRKAYAMVEKFQAEKT--CSSRALARKRQR 220
           VMSTMGLTSSVRKAYAMVEKFQA KT   SSR L  K+Q+
Sbjct: 432 VMSTMGLTSSVRKAYAMVEKFQAGKTSSSSSRVLPIKKQK 463

BLAST of Spg013960 vs. TAIR 10
Match: AT4G12740.1 (HhH-GPD base excision DNA repair family protein )

HSP 1 Score: 187.6 bits (475), Expect = 1.3e-47
Identity = 99/194 (51.03%), Postives = 131/194 (67.53%), Query Frame = 0

Query: 3   PSCSTCPVFDHCKALSISKHDSSVLVTDYPAKGIKTKQRHDYSAVCVVEILENQGTSELE 62
           PSCS+CPV   C+A S+S+ + ++ VTDYP K IK K RHD+  VCV+EI         +
Sbjct: 346 PSCSSCPVSSQCRAFSLSEENRTISVTDYPTKVIKAKPRHDFCCVCVLEI---HNLERNQ 405

Query: 63  QSSRFLLVKRPDEGLLAGLWEFPSVLLDGEADLSTRRESINNLLSQC--FGLEPKKNFEI 122
              RF+LVKRP++GLLAGLWEFPSV+L+ EAD +TRR +IN  L +   F +E KK   I
Sbjct: 406 SGGRFVLVKRPEQGLLAGLWEFPSVILNEEADSATRRNAINVYLKEAFRFHVELKKACTI 465

Query: 123 VIREDVGDFVHVFTHIRLKIYVEHLVLCLKGVFSVTMRSEGSKLFRRREKKSTLWKCVDN 182
           V RE++G+FVH+FTHIR K+YVE LV+ L G            LF+ + K +  WKCV +
Sbjct: 466 VSREELGEFVHIFTHIRRKVYVELLVVQLTG--------GTEDLFKGQAKDTLTWKCVSS 525

Query: 183 KVMSTMGLTSSVRK 195
            V+ST+GLTS+VRK
Sbjct: 526 DVLSTLGLTSAVRK 528

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038874808.13.8e-9784.86adenine DNA glycosylase isoform X1 [Benincasa hispida][more]
XP_038874810.13.8e-9784.86adenine DNA glycosylase isoform X3 [Benincasa hispida][more]
XP_038874809.13.8e-9784.86adenine DNA glycosylase isoform X2 [Benincasa hispida][more]
XP_022953220.11.1e-9683.93adenine DNA glycosylase [Cucurbita moschata][more]
KAG6576094.17.2e-9683.48Adenine DNA glycosylase, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
F4JRF41.9e-4651.03Adenine DNA glycosylase OS=Arabidopsis thaliana OX=3702 GN=MYH PE=3 SV=1[more]
Q99P215.7e-1131.07Adenine DNA glycosylase OS=Mus musculus OX=10090 GN=Mutyh PE=1 SV=2[more]
Q9UIF71.4e-0929.50Adenine DNA glycosylase OS=Homo sapiens OX=9606 GN=MUTYH PE=1 SV=1[more]
Q8R5G22.4e-0934.62Adenine DNA glycosylase OS=Rattus norvegicus OX=10116 GN=Mutyh PE=2 SV=1[more]
Q101591.5e-0827.17Adenine DNA glycosylase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) O... [more]
Match NameE-valueIdentityDescription
A0A6J1GP175.4e-9783.93Adenine DNA glycosylase OS=Cucurbita moschata OX=3662 GN=LOC111455831 PE=3 SV=1[more]
A0A6J1JU234.5e-9683.04Adenine DNA glycosylase OS=Cucurbita maxima OX=3661 GN=LOC111488366 PE=3 SV=1[more]
A0A5A7T8X35.9e-9685.00Adenine DNA glycosylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A0A0KC272.9e-9584.55Adenine DNA glycosylase OS=Cucumis sativus OX=3659 GN=Csa_6G088720 PE=3 SV=1[more]
A0A1S3CBT21.1e-9484.55Adenine DNA glycosylase OS=Cucumis melo OX=3656 GN=LOC103498904 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G12740.11.3e-4751.03HhH-GPD base excision DNA repair family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029119MutY, C-terminalPFAMPF14815NUDIX_4coord: 64..154
e-value: 2.0E-13
score: 50.3
IPR029119MutY, C-terminalCDDcd03431DNA_Glycosylase_Ccoord: 41..147
e-value: 7.46661E-21
score: 83.1551
NoneNo IPR availableGENE3D3.90.79.10Nucleoside Triphosphate Pyrophosphohydrolasecoord: 37..208
e-value: 7.7E-31
score: 108.5
IPR044298Adenine/Thymine-DNA glycosylasePANTHERPTHR42944ADENINE DNA GLYCOSYLASEcoord: 2..216
IPR015797NUDIX hydrolase-like domain superfamilySUPERFAMILY55811Nudixcoord: 32..144

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg013960.1Spg013960.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006284 base-excision repair
biological_process GO:0006298 mismatch repair
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005634 nucleus
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0034039 8-oxo-7,8-dihydroguanine DNA N-glycosylase activity
molecular_function GO:0035485 adenine/guanine mispair binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0032357 oxidized purine DNA binding
molecular_function GO:0000701 purine-specific mismatch base pair DNA N-glycosylase activity
molecular_function GO:0016798 hydrolase activity, acting on glycosyl bonds