Spg013857 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg013857
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like
Location	scaffold3: 42283739 .. 42285791 (-)
RNA-Seq Expression	Spg013857
Synteny	Spg013857

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGGCCTCAACTATAGCAAAAAAGAGAAGAAAAAAAAGCCCTTTAATGGAAGAATCAAAACAAGAGCCAAAGGAGATGGTGAAGTCTCCTCCAAGGCTCTATAGGAGGAAAAGTTGGACCAAGATGGATAAAAATGTTGCTTTCTTCTTTCTCATTTTGCATCTACTTTGTCTTTTGGCGCCCTTCGATTTCAGTTGGAGTGGTTTTTTGCTTTCCGTTGTGATAGGCAATACTACAGGTATGGCAATTAGTGTTTCGTTTCATAGAAATCTTGCACATCGGAGCTTCAAACTTCCCAAATGGCTCGAGTATTCGCTTGCTTATTGTGCATCTCATGCACTTCAGGTTCGATATTTTGAATCCTTTCTCTTAATTAAAAATGAACTAAGCTAGTGTACACATTATTCTTATTTTGTAGTGTAAAAATGATTATAAATTTATAACTATGTTTTATGTAGTAACTTGCATAATACCTTATATCATCAAATCAAAGAGTTAGCTTTTACTATTGTATAAAAAAAAGAGTGGCCTTGTGTGATTGCTGCATGTCAATAAAAGCATTGACAATGAGGCCCACATGGATTTTTTTTTTTTTTTTTTTTTTTTTTGGAACTAGAAGATGAACTTGCCATAAAACTAGTGAAAAAACTATAGTATCATTTTGCTAATTATCTTTGATCTCTTAACATATCTTTTATGTTTGGTAACATTTTTGTTGTTTTCTGAATTTTAGGGTGATCCAATTGATTGGGTAAGCACACACAGGTGTCATCATCGATATGTTGATACAGAAAGAGACCCACATAGTCCTATATATGGATTCTGGTTTAGTCAAATAACGTGGTTTTTTGATTCCTATAACTTGACTAGAAAAGTGTGCCCAAATTATTTCAATAATTTTCAAAAGGTAGAAAGAAATAGCTTCGTGTTGTTCTTGAAGCATGGGTATCCAGACAATGTCAACGACTTGGAGAGACAAGCTTTCTATAGGTTTATGCACAAAACATATATGCTACATCCACTTGCTCTTTCAGCATTGTTGTATGCCATCGGAGGATTTCATTTTATTCTATGGGGAATGGTAAAATGAATCTACTCTCATTCTTTTTCCTTTTAATTATATGAATGAAAGCAAATATAATATCGTTGAACTCAAAAAAAAAAAAAAAAAAAAAAATCTATTATAACCATGGGCCCATGACTATGATATATACTAGTTTTGGTAATTCTCTTGTATTAAATATTTTACTTACGAAAGTCTTTTTTTTTTTTACTATTCATAGTGCGTGAGGTACATTATGAGCTTGCATATAACATCTATGATAAATTCAGTATGTCATATATGGGGAAAACAACCATGGAATACTGGCGATTTGTCTCGAAACAATTGGTAAGATACTTATCCATCTCATTTTATCTCTACCCTTCACAATTCTCTCTCTTTCATCCAACCGTGCAAAACTACAGATTTCATTGCACCGGGAGCACCTAAAATTTCTCACAAGGGAAAGACGCTTTAAATAGGATTTAACATGACAAAAGTTTATATGGAAATCACTCACCTAACCTAGGACTTATTGATTAGAAAAAGTGAATAAAAGATTTGTTTATATCAATTTAATTTATTAAACATTAATAGCAATTAGAAAAGTGATTTTGGTTTAATATTATTTTTGTTATTAGGTTGGTGGCTTTATTTACCTTTGGAGAAGGATGGCATAATAACCACCATGCTTTTGAGTATTCAGCAAGGCATGGTCATGAATGGTGGCAAATTGACTTTGGTTGGTACATTATTAAGTTTCTTCATGTCATTGGACTGGCAACTAATGTGAAATTACCTTCTCCAAGCAAAAAGTTGGCAGCTTTGGACAAATCAAAATTCACCCTTCTTAAGCCTATGATTAATTAAGATCTATGTCATAATATTGGCACATTTATGAGAATGTGTGATAATGTAATGTTTTAATGATTTTTGTGTTGCAAGATGGTGTATTCCAAACATTATATTATAAGTATAATCTTATTATGGTATATGGTTTTATGTGCC

mRNA sequence

ATGAAGGCCTCAACTATAGCAAAAAAGAGAAGAAAAAAAAGCCCTTTAATGGAAGAATCAAAACAAGAGCCAAAGGAGATGGTGAAGTCTCCTCCAAGGCTCTATAGGAGGAAAAGTTGGACCAAGATGGATAAAAATGTTGCTTTCTTCTTTCTCATTTTGCATCTACTTTGTCTTTTGGCGCCCTTCGATTTCAGTTGGAGTGGTTTTTTGCTTTCCGTTGTGATAGGCAATACTACAGGTATGGCAATTAGTGTTTCGTTTCATAGAAATCTTGCACATCGGAGCTTCAAACTTCCCAAATGGCTCGAGTATTCGCTTGCTTATTGTGCATCTCATGCACTTCAGGGTGATCCAATTGATTGGGTAAGCACACACAGGTGTCATCATCGATATGTTGATACAGAAAGAGACCCACATAGTCCTATATATGGATTCTGGTTTAGTCAAATAACGTGGTTTTTTGATTCCTATAACTTGACTAGAAAAGTGTGCCCAAATTATTTCAATAATTTTCAAAAGGTAGAAAGAAATAGCTTCGTGTTGTTCTTGAAGCATGGGTATCCAGACAATGTCAACGACTTGGAGAGACAAGCTTTCTATAGGTTTATGCACAAAACATATATGCTACATCCACTTGCTCTTTCAGCATTGTTGTATGCCATCGGAGGATTTCATTTTATTCTATGGGGAATGTGCGTGAGGTACATTATGAGCTTGCATATAACATCTATGATAAATTCAGTATGTCATATATGGGGAAAACAACCATGGAATACTGGCGATTTGTCTCGAAACAATTGGTTGGTGGCTTTATTTACCTTTGGAGAAGGATGGCATAATAACCACCATGCTTTTGAGTATTCAGCAAGGCATGGTCATGAATGGTGGCAAATTGACTTTGGTTGGTACATTATTAAGTTTCTTCATGTCATTGGACTGGCAACTAATGTGAAATTACCTTCTCCAAGCAAAAAGTTGGCAGCTTTGGACAAATCAAAATTCACCCTTCTTAAGCCTATGATTAATTAA

Coding sequence (CDS)

ATGAAGGCCTCAACTATAGCAAAAAAGAGAAGAAAAAAAAGCCCTTTAATGGAAGAATCAAAACAAGAGCCAAAGGAGATGGTGAAGTCTCCTCCAAGGCTCTATAGGAGGAAAAGTTGGACCAAGATGGATAAAAATGTTGCTTTCTTCTTTCTCATTTTGCATCTACTTTGTCTTTTGGCGCCCTTCGATTTCAGTTGGAGTGGTTTTTTGCTTTCCGTTGTGATAGGCAATACTACAGGTATGGCAATTAGTGTTTCGTTTCATAGAAATCTTGCACATCGGAGCTTCAAACTTCCCAAATGGCTCGAGTATTCGCTTGCTTATTGTGCATCTCATGCACTTCAGGGTGATCCAATTGATTGGGTAAGCACACACAGGTGTCATCATCGATATGTTGATACAGAAAGAGACCCACATAGTCCTATATATGGATTCTGGTTTAGTCAAATAACGTGGTTTTTTGATTCCTATAACTTGACTAGAAAAGTGTGCCCAAATTATTTCAATAATTTTCAAAAGGTAGAAAGAAATAGCTTCGTGTTGTTCTTGAAGCATGGGTATCCAGACAATGTCAACGACTTGGAGAGACAAGCTTTCTATAGGTTTATGCACAAAACATATATGCTACATCCACTTGCTCTTTCAGCATTGTTGTATGCCATCGGAGGATTTCATTTTATTCTATGGGGAATGTGCGTGAGGTACATTATGAGCTTGCATATAACATCTATGATAAATTCAGTATGTCATATATGGGGAAAACAACCATGGAATACTGGCGATTTGTCTCGAAACAATTGGTTGGTGGCTTTATTTACCTTTGGAGAAGGATGGCATAATAACCACCATGCTTTTGAGTATTCAGCAAGGCATGGTCATGAATGGTGGCAAATTGACTTTGGTTGGTACATTATTAAGTTTCTTCATGTCATTGGACTGGCAACTAATGTGAAATTACCTTCTCCAAGCAAAAAGTTGGCAGCTTTGGACAAATCAAAATTCACCCTTCTTAAGCCTATGATTAATTAA

Protein sequence

MKASTIAKKRRKKSPLMEESKQEPKEMVKSPPRLYRRKSWTKMDKNVAFFFLILHLLCLLAPFDFSWSGFLLSVVIGNTTGMAISVSFHRNLAHRSFKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRYVDTERDPHSPIYGFWFSQITWFFDSYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNVNDLERQAFYRFMHKTYMLHPLALSALLYAIGGFHFILWGMCVRYIMSLHITSMINSVCHIWGKQPWNTGDLSRNNWLVALFTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVIGLATNVKLPSPSKKLAALDKSKFTLLKPMIN

Homology

[Close]

BLAST of Spg013857 vs. NCBI nr
Match: XP_038899717.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 586.3 bits (1510), Expect = 1.8e-163
Identity = 266/327 (81.35%), Postives = 298/327 (91.13%), Query Frame = 0


Query: 18  EESKQEPKEMV-KSPPRLYRRKSWTKMDKNVAFFFLILHLLCLLAPFDFSWSGFLLSVVI 77
           E SK+ PKEMV KSPPRL+RRK WTK D+NVAF+FLILHLLC+LAPF FSW+   L++ +
Sbjct: 3   EASKEGPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLILHLLCILAPFYFSWTCLFLALGL 62

Query: 78  GNTTGMAISVSFHRNLAHRSFKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRYVDTE 137
              TGM ISVS+HRNLAHRSFKLPKWLEYSLAY A+HA+QGDPIDWVSTHRCHHRYVD+E
Sbjct: 63  SYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRYVDSE 122

Query: 138 RDPHSPIYGFWFSQITWFFDSYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNVNDLE 197
           RDPHSPIYGFWFSQ+TWFFDSYNLTRKVCPNYFNNFQKVERN+F+ ++KHG+PDNV DLE
Sbjct: 123 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQKVERNAFMFYMKHGHPDNVTDLE 182

Query: 198 RQAFYRFMHKTYMLHPLALSALLYAIGGFHFILWGMCVRYIMSLHITSMINSVCHIWGKQ 257
           +Q FYRF+HKTY LHPLALS LLYAIGG  FI+WGMCVR++ SLH+TSM+NSVCHIWGKQ
Sbjct: 183 KQRFYRFIHKTYWLHPLALSVLLYAIGGVPFIIWGMCVRFVFSLHVTSMVNSVCHIWGKQ 242

Query: 258 PWNTGDLSRNNWLVALFTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVIGLAT 317
           PWNTGDLS+NNW+VA+ TFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFL VIGLAT
Sbjct: 243 PWNTGDLSKNNWMVAILTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLQVIGLAT 302

Query: 318 NVKLPSPSKKLAALDKSKFTLLKPMIN 344
           NVKLPS  +KLAAL+KSK T LKPMI+
Sbjct: 303 NVKLPSEKQKLAALNKSKPTFLKPMID 329

[Close]

BLAST of Spg013857 vs. NCBI nr
Match: XP_008451258.1 (PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucumis melo])

HSP 1 Score: 576.6 bits (1485), Expect = 1.4e-160
Identity = 262/327 (80.12%), Postives = 294/327 (89.91%), Query Frame = 0


Query: 18  EESKQEPKEMV-KSPPRLYRRKSWTKMDKNVAFFFLILHLLCLLAPFDFSWSGFLLSVVI 77
           E SK+E KEMV  SP RL++RK WTK DKNVAF+FL+LHLLC+LAPF FSW+   LS+ +
Sbjct: 3   EASKEESKEMVMNSPSRLFKRKKWTKFDKNVAFYFLLLHLLCILAPFYFSWTCLFLSLGL 62

Query: 78  GNTTGMAISVSFHRNLAHRSFKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRYVDTE 137
            N TGM ISVS+HRNLAHRSFKLPKWLEYSLAY A+HA+QGDPIDWVSTHRCHHR+VD+E
Sbjct: 63  ANLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSE 122

Query: 138 RDPHSPIYGFWFSQITWFFDSYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNVNDLE 197
           RDPHSPIYGFWFSQ+TWFFDSYNLTRKVCPNYFNNFQKVERN+F+ ++KHGY DNV DLE
Sbjct: 123 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQKVERNAFMFYMKHGYADNVTDLE 182

Query: 198 RQAFYRFMHKTYMLHPLALSALLYAIGGFHFILWGMCVRYIMSLHITSMINSVCHIWGKQ 257
           +Q FYRF+HKTY LHPLALS LLYAIGG  FI+WGMCVR+++SLH TSMINSVCHIWG Q
Sbjct: 183 KQRFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVISLHFTSMINSVCHIWGNQ 242

Query: 258 PWNTGDLSRNNWLVALFTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVIGLAT 317
           PWNTGDLS+NNW+VA+FTFGEGWHNNHHAFEYSARHGHEWWQID+GWYIIKFL VIG AT
Sbjct: 243 PWNTGDLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGFAT 302

Query: 318 NVKLPSPSKKLAALDKSKFTLLKPMIN 344
           NVKLPS  +K AAL+KSK T LKPMI+
Sbjct: 303 NVKLPSEKQKWAALNKSKPTFLKPMID 329

[Close]

BLAST of Spg013857 vs. NCBI nr
Match: XP_023549408.1 (delta-9 acyl-lipid desaturase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 572.0 bits (1473), Expect = 3.4e-159
Identity = 253/315 (80.32%), Postives = 287/315 (91.11%), Query Frame = 0


Query: 20  SKQEPKEMVKSPPRLYRRKSWTKMDKNVAFFFLILHLLCLLAPFDFSWSGFLLSVVIGNT 79
           +K+ P  MV+SPP L+ RK WTK D+NVA +FL+LHLLC+LAPF F+W+ F L++++GN 
Sbjct: 17  TKKHPNNMVQSPPMLFTRKKWTKFDRNVASYFLLLHLLCILAPFYFTWTCFFLTIILGNL 76

Query: 80  TGMAISVSFHRNLAHRSFKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRYVDTERDP 139
           TGMAISVS+HRNLAHR+FKLPKWLEYSLAYCA+HA+QGDPIDWVSTHRCHHRYVDTERDP
Sbjct: 77  TGMAISVSYHRNLAHRAFKLPKWLEYSLAYCAAHAIQGDPIDWVSTHRCHHRYVDTERDP 136

Query: 140 HSPIYGFWFSQITWFFDSYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNVNDLERQA 199
           HSPIYGFWFSQ+TWFFDSYNLTRKVCPNYF NF+KVERN F+ F+KHGYPDNV DLE+Q 
Sbjct: 137 HSPIYGFWFSQMTWFFDSYNLTRKVCPNYFKNFEKVERNMFMFFMKHGYPDNVRDLEKQW 196

Query: 200 FYRFMHKTYMLHPLALSALLYAIGGFHFILWGMCVRYIMSLHITSMINSVCHIWGKQPWN 259
           FYRFMHKTY LHPLAL+ LLYA+GG  +++WGMCVR+++SLH+TSMINSVCHIWGKQPWN
Sbjct: 197 FYRFMHKTYWLHPLALAGLLYAVGGVPYVIWGMCVRFVVSLHVTSMINSVCHIWGKQPWN 256

Query: 260 TGDLSRNNWLVALFTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVIGLATNVK 319
           TGDLSRNNWLVAL TFGEGWHNNHHAFEYSARHGH WWQIDFGWYIIKFL VIGLAT VK
Sbjct: 257 TGDLSRNNWLVALITFGEGWHNNHHAFEYSARHGHVWWQIDFGWYIIKFLQVIGLATCVK 316

Query: 320 LPSPSKKLAALDKSK 335
           LPS +  LAAL+KSK
Sbjct: 317 LPSQTHNLAALNKSK 331

[Close]

BLAST of Spg013857 vs. NCBI nr
Match: XP_011659770.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic [Cucumis sativus])

HSP 1 Score: 570.1 bits (1468), Expect = 1.3e-158
Identity = 260/326 (79.75%), Postives = 295/326 (90.49%), Query Frame = 0


Query: 20  SKQEPKEMV-KSPPRLYRRKSWTKMDKNVAFFFLILHLLCLLAP-FDFSWSGFLLSVVIG 79
           SK++PKEMV  SP RL++RK WTK DKNVAF+FL+LHLL +LAP F FSW   LLS+ + 
Sbjct: 5   SKEDPKEMVMSSPSRLFKRKKWTKFDKNVAFYFLLLHLLSILAPLFYFSWGCLLLSLGLA 64

Query: 80  NTTGMAISVSFHRNLAHRSFKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRYVDTER 139
           N TGMAISVS+HRNLAHRSFKLPKWLEYSLAY A+HA+QGDPIDWVSTHRCHHR+VD+ER
Sbjct: 65  NLTGMAISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSER 124

Query: 140 DPHSPIYGFWFSQITWFFDSYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNVNDLER 199
           DPHSPIYGFWFSQ+TWFFDSYNLTRKVCPNYFNN+QKVERN F+ ++KHGYPDNV DLE+
Sbjct: 125 DPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVTDLEK 184

Query: 200 QAFYRFMHKTYMLHPLALSALLYAIGGFHFILWGMCVRYIMSLHITSMINSVCHIWGKQP 259
           Q FYRF+HKTY LHPLALS LLYA+GG  FI+WG+CVR+++SLH TSMINSVCHIWG QP
Sbjct: 185 QKFYRFIHKTYWLHPLALSLLLYALGGLPFIIWGVCVRFVLSLHFTSMINSVCHIWGNQP 244

Query: 260 WNTGDLSRNNWLVALFTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVIGLATN 319
           WNTGDLS+NNW+VA+FTFGEGWHNNHHAFEYSARHGHEWWQID GWYIIKFL VIGLATN
Sbjct: 245 WNTGDLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDNGWYIIKFLQVIGLATN 304

Query: 320 VKLPSPSKKLAALDKSKFTLLKPMIN 344
           VKLPS  +K+AAL+KSK T L+PMI+
Sbjct: 305 VKLPSEKQKMAALNKSKPTFLRPMID 330

[Close]

BLAST of Spg013857 vs. NCBI nr
Match: XP_022953867.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 569.3 bits (1466), Expect = 2.2e-158
Identity = 252/318 (79.25%), Postives = 289/318 (90.88%), Query Frame = 0


Query: 17  MEESKQEPKEMVKSPPRLYRRKSWTKMDKNVAFFFLILHLLCLLAPFDFSWSGFLLSVVI 76
           ME SKQ P  MV+SPP L+ RK WTK D+NVA +FL+LHLLC+LAPF F+W+ F L++++
Sbjct: 1   MEASKQHP-NMVQSPPMLFTRKKWTKFDRNVASYFLLLHLLCILAPFYFTWTSFFLTIIL 60

Query: 77  GNTTGMAISVSFHRNLAHRSFKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRYVDTE 136
            N TGMAISVS+HRNLAHR+FKLPKWLEYSLAYCA+HA+QGDPIDWVSTHRCHHRYVDTE
Sbjct: 61  ANLTGMAISVSYHRNLAHRAFKLPKWLEYSLAYCAAHAIQGDPIDWVSTHRCHHRYVDTE 120

Query: 137 RDPHSPIYGFWFSQITWFFDSYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNVNDLE 196
           RDPHSPIYGFWFSQ+TWFFDSYNLTRKVCPNYF NF+KVERN F+ F+KHGYPDNV DLE
Sbjct: 121 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFKNFEKVERNMFMFFMKHGYPDNVRDLE 180

Query: 197 RQAFYRFMHKTYMLHPLALSALLYAIGGFHFILWGMCVRYIMSLHITSMINSVCHIWGKQ 256
           +Q FYRFM KTY LHPLAL+ LLYA+GG  +++WGMCVR+++SLHITSM+NSVCHIWG Q
Sbjct: 181 KQWFYRFMRKTYWLHPLALAGLLYAVGGVPYVIWGMCVRFVLSLHITSMVNSVCHIWGTQ 240

Query: 257 PWNTGDLSRNNWLVALFTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVIGLAT 316
           PWNTGDLSRNNW+VAL TFGEGWHNNHHAFEYSARHGHEWW+ID+GWY+IKFL VIGLAT
Sbjct: 241 PWNTGDLSRNNWMVALITFGEGWHNNHHAFEYSARHGHEWWEIDYGWYVIKFLQVIGLAT 300

Query: 317 NVKLPSPSKKLAALDKSK 335
            VKLPS ++KLAAL+KSK
Sbjct: 301 CVKLPSQTQKLAALNKSK 317

[Close]

BLAST of Spg013857 vs. ExPASy Swiss-Prot
Match: Q949X0 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3 PE=1 SV=2)

HSP 1 Score: 358.6 bits (919), Expect = 7.8e-98
Identity = 162/290 (55.86%), Postives = 205/290 (70.69%), Query Frame = 0


Query: 38  KSWTKMDKNVAFFFLILHLLCLLAPFDFSWSGFLLSVVIGNTTG-MAISVSFHRNLAHRS 97
           + W  MD       L +HLL LLAPF F+W    ++  +   TG + I++SFHRNL+H++
Sbjct: 96  REWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSFHRNLSHKA 155

Query: 98  FKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRYVDTERDPHSPIYGFWFSQITWFFD 157
           FKLPKWLEY  AYC + ALQG+PIDWVSTHR HH++ D++RDPHSP+ GFWFS + W FD
Sbjct: 156 FKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWFSHMNWMFD 215

Query: 158 SYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNVNDLERQAFYRFMHKTYMLHPLALS 217
           +  +T++                       G P+NV DLE+Q FYRF+  TY+LHPLAL+
Sbjct: 216 TNTITQRC----------------------GEPNNVGDLEKQPFYRFLRTTYILHPLALA 275

Query: 218 ALLYAIGGFHFILWGMCVRYIMSLHITSMINSVCHIWGKQPWNTGDLSRNNWLVALFTFG 277
             LYA+GGF FI+WGM VR +   HIT ++NS CH+WGKQ WNTGDLS+NNW VA   FG
Sbjct: 276 VALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSKNNWWVAALAFG 335

Query: 278 EGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVIGLATNVKLPSPSKK 327
           EGWHNNHHAFE+SARHG EWWQ+D  WY++KFL  IGLAT+VKLPS ++K
Sbjct: 336 EGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLPSEAQK 363

[Close]

BLAST of Spg013857 vs. ExPASy Swiss-Prot
Match: O65797 (Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 2.5e-88
Identity = 152/314 (48.41%), Postives = 201/314 (64.01%), Query Frame = 0


Query: 19  ESKQEPKEMVKSPPRLYRRK------SWTKMDKNVAFFFLILHLLCLLAPFDFSWSGFLL 78
           E ++  K+M      + R+K       W ++D   AF  L +H LCLLAPF+F+W    +
Sbjct: 7   EKEENNKKMAADKAEMGRKKRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRV 66

Query: 79  SVVIGNTTGMAISVSFHRNLAHRSFKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRY 138
           ++++    G+ I+VS+HRNLAHRSFK+PKWLEY  AYC   A+QGDPIDWVSTHR HH++
Sbjct: 67  ALIVYTVGGLGITVSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQF 126

Query: 139 VDTERDPHSPIYGFWFSQITWFFDSYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNV 198
            D++RDPHSP  GFWFS + W FD+  L  K                       G   NV
Sbjct: 127 TDSDRDPHSPNEGFWFSHLLWLFDTGYLVEKC----------------------GRRTNV 186

Query: 199 NDLERQAFYRFMHKTYMLHPLALSALLYAIGGFHFILWGMCVRYIMSLHITSMINSVCHI 258
            DL+RQ +Y+F+ +T + H L    LLY  GG  F+ WGM +   M  H+T +INS+CH+
Sbjct: 187 EDLKRQWYYKFLQRTVLYHILTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCLINSLCHV 246

Query: 259 WGKQPWNTGDLSRNNWLVALFTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVI 318
           WG + W T D SRN W +++F+FGE WHNNHHAFE SAR G EWWQID  WYI++FL +I
Sbjct: 247 WGSRTWKTNDTSRNVWWLSVFSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFLEII 298

Query: 319 GLATNVKLPSPSKK 327
           GLAT+VKLPS S++
Sbjct: 307 GLATDVKLPSESQR 298

[Close]

BLAST of Spg013857 vs. ExPASy Swiss-Prot
Match: Q9FV68 (Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii OX=28973 PE=1 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 4.8e-87
Identity = 160/299 (53.51%), Postives = 192/299 (64.21%), Query Frame = 0


Query: 37  RKSWTKMDKNVAFFFLILHLLCLLAPFDFSWSGFLLS-VVIGNTTG-MAISVSFHRNLAH 96
           R+ W   D   A   L+LH L  +APF FSW  F +S +++G  +G + I++ FHR L H
Sbjct: 78  RRKWNPRDIQNAVTLLVLHALAAMAPFYFSWDAFWISFILLGFASGVLGITLCFHRCLTH 137

Query: 97  RSFKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRYVDTERDPHSPIYGFWFSQITWF 156
             FKLPK +EY  AYC S ALQGDP++WVS HR HH++VDTERD HSP  GFWF  I W 
Sbjct: 138 GGFKLPKLVEYFFAYCGSLALQGDPMEWVSNHRYHHQFVDTERDVHSPTQGFWFCHIGWV 197

Query: 157 FDSYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNVNDLERQAFYRFMHKTYMLHPLA 216
            D      K                     + G  +NVNDL++QAFYRF+ KTYM H LA
Sbjct: 198 LDKDLFVEK---------------------RGGRRNNVNDLKKQAFYRFLQKTYMYHQLA 257

Query: 217 LSALLYAIGGFHFILWGMCVRYIMSLHITSMINSVCHIWGKQPWNTGDLSRNNWLVALFT 276
           L ALLY +GGF +I+WGM  R +   H T  INSVCH WG +PWNTGDLS NN  VAL  
Sbjct: 258 LIALLYYVGGFPYIVWGMGFRLVFMFHSTFAINSVCHKWGGRPWNTGDLSTNNMFVALCA 317

Query: 277 FGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVIGLATNVKLPSPSKKLAALDKS 334
           FGEGWHNNHHAFE SARHG EWW+ID  WY+I+ L  IGLATNVKLP+ ++K     KS
Sbjct: 318 FGEGWHNNHHAFEQSARHGLEWWEIDVTWYVIRTLQAIGLATNVKLPTEAQKQKLKAKS 355

[Close]

BLAST of Spg013857 vs. ExPASy Swiss-Prot
Match: P0DOW2 (Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 6.2e-87
Identity = 153/290 (52.76%), Postives = 186/290 (64.14%), Query Frame = 0


Query: 38  KSWTKMDKNVAFFFLILHLLCLLAPFDFSWSGFLLSVVIGNTTGM-AISVSFHRNLAHRS 97
           + W   D     F   LH+LCL AP  FSW  F +   +    G+   ++SFHRNL HRS
Sbjct: 38  RKWNSTDVFHILFIGGLHVLCLFAPSTFSWKSFWVCFALYAICGVFGTTLSFHRNLTHRS 97

Query: 98  FKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRYVDTERDPHSPIYGFWFSQITWFFD 157
           FKLPK+LEY  AY   HALQGDP+ WVSTHR HH+Y DT  DPHSPI GFWF  I W FD
Sbjct: 98  FKLPKYLEYFFAYVGLHALQGDPVWWVSTHRYHHKYTDTYLDPHSPIEGFWFCHIFWLFD 157

Query: 158 SYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNVNDLERQAFYRFMHKTYMLHPLALS 217
           S  +  K C  Y                     +N  DL +Q++YRF+ +T++ H    +
Sbjct: 158 SKYIIEK-CGRY---------------------ENAGDLMKQSYYRFLERTFVYHVYLQA 217

Query: 218 ALLYAIGGFHFILWGMCVRYIMSLHITSMINSVCHIWGKQPWNTGDLSRNNWLVALFTFG 277
           ALLY  GGF FI+WGM VR I+  H++ ++NSVCH WG +PWNTGDLS NNW +A+ T G
Sbjct: 218 ALLYLFGGFPFIVWGMAVRTILGFHLSWLVNSVCHRWGNRPWNTGDLSTNNWFIAMLTSG 277

Query: 278 EGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVIGLATNVKLPSPSKK 327
           EGWHNNHHAFEYSARHG EWWQID  WYIIK L  +GLAT++K+PS   K
Sbjct: 278 EGWHNNHHAFEYSARHGIEWWQIDTTWYIIKLLEYLGLATDIKVPSEIHK 305

[Close]

BLAST of Spg013857 vs. ExPASy Swiss-Prot
Match: Q9LVZ3 (Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3.2 PE=2 SV=3)

HSP 1 Score: 317.0 bits (811), Expect = 2.6e-85
Identity = 146/293 (49.83%), Postives = 190/293 (64.85%), Query Frame = 0


Query: 33  RLYRRKSWTKMDKNVAFFFLILHLLCLLAPFDFSWSGFLLSVVIGNTTGMAISVSFHRNL 92
           R +  +SWT  D +    F+  HLL LLAPF FSW  F +   +    G+ I++S+HRNL
Sbjct: 84  RSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGICITLSYHRNL 143

Query: 93  AHRSFKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRYVDTERDPHSPIYGFWFSQIT 152
           +HRSF LPKWLEY  AY    A QGDPI+WVS HR HH++ +T+RDPHSP  GFWFS + 
Sbjct: 144 SHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQGFWFSHMA 203

Query: 153 WFFDSYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNVNDLERQAFYRFMHKTYMLHP 212
           W FD+ ++                          G  +NV+DL RQ FYRF+ +T +LH 
Sbjct: 204 WIFDTSSILENC----------------------GGEENVDDLVRQPFYRFLQRTVLLHM 263

Query: 213 LALSALLYAIGGFHFILWGMCVRYIMSLHITSMINSVCHIWGKQPWNTGDLSRNNWLVAL 272
           +A S L Y  GG   ++WG+ +   + LH+T ++NSVCHIWG + WNT D S+NNW VA+
Sbjct: 264 MAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSDFSKNNWWVAI 323

Query: 273 FTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVIGLATNVKLPSPSK 326
            T GEGWHNNHHAFE+SARHG EWWQ+D  W +I+FL  IGLATNVKLP+ ++
Sbjct: 324 LTLGEGWHNNHHAFEFSARHGLEWWQLDITWCLIRFLEAIGLATNVKLPTETQ 354

[Close]

BLAST of Spg013857 vs. ExPASy TrEMBL
Match: A0A1S3BS49 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103492608 PE=3 SV=1)

HSP 1 Score: 576.6 bits (1485), Expect = 6.7e-161
Identity = 262/327 (80.12%), Postives = 294/327 (89.91%), Query Frame = 0


Query: 18  EESKQEPKEMV-KSPPRLYRRKSWTKMDKNVAFFFLILHLLCLLAPFDFSWSGFLLSVVI 77
           E SK+E KEMV  SP RL++RK WTK DKNVAF+FL+LHLLC+LAPF FSW+   LS+ +
Sbjct: 3   EASKEESKEMVMNSPSRLFKRKKWTKFDKNVAFYFLLLHLLCILAPFYFSWTCLFLSLGL 62

Query: 78  GNTTGMAISVSFHRNLAHRSFKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRYVDTE 137
            N TGM ISVS+HRNLAHRSFKLPKWLEYSLAY A+HA+QGDPIDWVSTHRCHHR+VD+E
Sbjct: 63  ANLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSE 122

Query: 138 RDPHSPIYGFWFSQITWFFDSYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNVNDLE 197
           RDPHSPIYGFWFSQ+TWFFDSYNLTRKVCPNYFNNFQKVERN+F+ ++KHGY DNV DLE
Sbjct: 123 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQKVERNAFMFYMKHGYADNVTDLE 182

Query: 198 RQAFYRFMHKTYMLHPLALSALLYAIGGFHFILWGMCVRYIMSLHITSMINSVCHIWGKQ 257
           +Q FYRF+HKTY LHPLALS LLYAIGG  FI+WGMCVR+++SLH TSMINSVCHIWG Q
Sbjct: 183 KQRFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVISLHFTSMINSVCHIWGNQ 242

Query: 258 PWNTGDLSRNNWLVALFTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVIGLAT 317
           PWNTGDLS+NNW+VA+FTFGEGWHNNHHAFEYSARHGHEWWQID+GWYIIKFL VIG AT
Sbjct: 243 PWNTGDLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGFAT 302

Query: 318 NVKLPSPSKKLAALDKSKFTLLKPMIN 344
           NVKLPS  +K AAL+KSK T LKPMI+
Sbjct: 303 NVKLPSEKQKWAALNKSKPTFLKPMID 329

[Close]

BLAST of Spg013857 vs. ExPASy TrEMBL
Match: A0A0A0KAB1 (FA_desaturase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G376370 PE=3 SV=1)

HSP 1 Score: 571.2 bits (1471), Expect = 2.8e-159
Identity = 259/327 (79.20%), Postives = 294/327 (89.91%), Query Frame = 0


Query: 18  EESKQEPKEMV-KSPPRLYRRKSWTKMDKNVAFFFLILHLLCLLAPFDFSWSGFLLSVVI 77
           E SK++PKEMV KSPPRL+RRK WTK D+NVAF+FL LHLLC+LAPF FSW+  LL++ +
Sbjct: 3   EVSKEKPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLALGL 62

Query: 78  GNTTGMAISVSFHRNLAHRSFKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRYVDTE 137
              TGM ISVS+HRNLAHRSFKLPKWLEYSLAY A+HA+QGDPIDWVSTHRCHHR+VD+ 
Sbjct: 63  SYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVDSA 122

Query: 138 RDPHSPIYGFWFSQITWFFDSYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNVNDLE 197
           RDPHSPIYGFWFSQ+TWFFDSYNLTRKVCPNYFNN+QKVERN F+ ++KHGYPDNV DLE
Sbjct: 123 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVTDLE 182

Query: 198 RQAFYRFMHKTYMLHPLALSALLYAIGGFHFILWGMCVRYIMSLHITSMINSVCHIWGKQ 257
           +Q FYRF+HKTY LHPLALS LLYAIGG  FI+WGMCVR++ SLH+TSM+NSVCHIWG Q
Sbjct: 183 KQKFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWGNQ 242

Query: 258 PWNTGDLSRNNWLVALFTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVIGLAT 317
           PWNTGDLS+NNW+VAL TFGEGWHNNHHAFEYSARHGHEWWQID+GWY+I+FL VIGLAT
Sbjct: 243 PWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGLAT 302

Query: 318 NVKLPSPSKKLAALDKSKFTLLKPMIN 344
           NVKLPS  +KLAAL+KSK   LK MI+
Sbjct: 303 NVKLPSEKQKLAALNKSKPIFLKLMID 329

[Close]

BLAST of Spg013857 vs. ExPASy TrEMBL
Match: A0A6J1GPG5 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111456275 PE=3 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 1.1e-158
Identity = 252/318 (79.25%), Postives = 289/318 (90.88%), Query Frame = 0


Query: 17  MEESKQEPKEMVKSPPRLYRRKSWTKMDKNVAFFFLILHLLCLLAPFDFSWSGFLLSVVI 76
           ME SKQ P  MV+SPP L+ RK WTK D+NVA +FL+LHLLC+LAPF F+W+ F L++++
Sbjct: 1   MEASKQHP-NMVQSPPMLFTRKKWTKFDRNVASYFLLLHLLCILAPFYFTWTSFFLTIIL 60

Query: 77  GNTTGMAISVSFHRNLAHRSFKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRYVDTE 136
            N TGMAISVS+HRNLAHR+FKLPKWLEYSLAYCA+HA+QGDPIDWVSTHRCHHRYVDTE
Sbjct: 61  ANLTGMAISVSYHRNLAHRAFKLPKWLEYSLAYCAAHAIQGDPIDWVSTHRCHHRYVDTE 120

Query: 137 RDPHSPIYGFWFSQITWFFDSYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNVNDLE 196
           RDPHSPIYGFWFSQ+TWFFDSYNLTRKVCPNYF NF+KVERN F+ F+KHGYPDNV DLE
Sbjct: 121 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFKNFEKVERNMFMFFMKHGYPDNVRDLE 180

Query: 197 RQAFYRFMHKTYMLHPLALSALLYAIGGFHFILWGMCVRYIMSLHITSMINSVCHIWGKQ 256
           +Q FYRFM KTY LHPLAL+ LLYA+GG  +++WGMCVR+++SLHITSM+NSVCHIWG Q
Sbjct: 181 KQWFYRFMRKTYWLHPLALAGLLYAVGGVPYVIWGMCVRFVLSLHITSMVNSVCHIWGTQ 240

Query: 257 PWNTGDLSRNNWLVALFTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVIGLAT 316
           PWNTGDLSRNNW+VAL TFGEGWHNNHHAFEYSARHGHEWW+ID+GWY+IKFL VIGLAT
Sbjct: 241 PWNTGDLSRNNWMVALITFGEGWHNNHHAFEYSARHGHEWWEIDYGWYVIKFLQVIGLAT 300

Query: 317 NVKLPSPSKKLAALDKSK 335
            VKLPS ++KLAAL+KSK
Sbjct: 301 CVKLPSQTQKLAALNKSK 317

[Close]

BLAST of Spg013857 vs. ExPASy TrEMBL
Match: A0A6J1JUL6 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111488510 PE=3 SV=1)

HSP 1 Score: 565.8 bits (1457), Expect = 1.2e-157
Identity = 252/318 (79.25%), Postives = 286/318 (89.94%), Query Frame = 0


Query: 17  MEESKQEPKEMVKSPPRLYRRKSWTKMDKNVAFFFLILHLLCLLAPFDFSWSGFLLSVVI 76
           ME SKQ P  MV+SPP L+ RK WTK D+NVA +FL+LHLLC+LAPF F+W+ F L++++
Sbjct: 1   MEASKQHP-NMVQSPPMLFTRKKWTKFDRNVASYFLLLHLLCILAPFYFTWTCFFLTIIL 60

Query: 77  GNTTGMAISVSFHRNLAHRSFKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRYVDTE 136
            N TGMAISVS+HRNLAHR+FKLPKWLEYSLAYCA+HA+QGDPIDWVSTHRCHHRYVDTE
Sbjct: 61  ANLTGMAISVSYHRNLAHRAFKLPKWLEYSLAYCAAHAIQGDPIDWVSTHRCHHRYVDTE 120

Query: 137 RDPHSPIYGFWFSQITWFFDSYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNVNDLE 196
           RDPHSPIYGFWFSQ+TWFFDSYNLTRKVCPNYF NF+KVERN F+ F+KHGYPDNV DLE
Sbjct: 121 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFKNFEKVERNMFMFFMKHGYPDNVRDLE 180

Query: 197 RQAFYRFMHKTYMLHPLALSALLYAIGGFHFILWGMCVRYIMSLHITSMINSVCHIWGKQ 256
           +Q FYRFM KTY LHPLAL+ LLYA GG  +++WGMCVR+++SLH+TSM+NSVCHIWG Q
Sbjct: 181 KQWFYRFMRKTYWLHPLALAGLLYAAGGIPYVIWGMCVRFVLSLHVTSMVNSVCHIWGNQ 240

Query: 257 PWNTGDLSRNNWLVALFTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVIGLAT 316
           PWNTGDLSRNNW+VAL TFGEGWHNNHHAFEYSARHGHEWWQID+GWYIIKFL VIGLAT
Sbjct: 241 PWNTGDLSRNNWMVALITFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGLAT 300

Query: 317 NVKLPSPSKKLAALDKSK 335
            VKLPS +  LAAL+KSK
Sbjct: 301 CVKLPSQTHNLAALNKSK 317

[Close]

BLAST of Spg013857 vs. ExPASy TrEMBL
Match: A0A1S3BQG5 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103492609 PE=3 SV=1)

HSP 1 Score: 557.0 bits (1434), Expect = 5.5e-155
Identity = 250/328 (76.22%), Postives = 289/328 (88.11%), Query Frame = 0


Query: 18  EESKQEPKEMV--KSPPRLYRRKSWTKMDKNVAFFFLILHLLCLLAPFDFSWSGFLLSVV 77
           E SK++PKE++  KSPP+L+RRK WTK D+N AF+FL  HLLC+LAPF FSW+  LL++ 
Sbjct: 4   EVSKEKPKEIILMKSPPKLFRRKKWTKFDRNFAFYFLTFHLLCILAPFCFSWTRLLLALG 63

Query: 78  IGNTTGMAISVSFHRNLAHRSFKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRYVDT 137
           +   +GM ISVS+HRNLAHRSFKLPKW EYSLAY A+HA+QGDPIDWVSTHRCHHR+VD+
Sbjct: 64  LSCLSGMGISVSYHRNLAHRSFKLPKWFEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDS 123

Query: 138 ERDPHSPIYGFWFSQITWFFDSYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNVNDL 197
           ERDPHSPIYGFWFSQ+TW FDSYNLTRKVCPNYFNN+QK ERN F+L++KHGYPDNV DL
Sbjct: 124 ERDPHSPIYGFWFSQMTWLFDSYNLTRKVCPNYFNNYQKAERNIFMLYMKHGYPDNVPDL 183

Query: 198 ERQAFYRFMHKTYMLHPLALSALLYAIGGFHFILWGMCVRYIMSLHITSMINSVCHIWGK 257
           E+Q FYRF+HKTY LHPLALS LLYAIGG  FI+WGMCVR++ S+H+TSM+NSVCH+WG 
Sbjct: 184 EKQWFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSVHVTSMVNSVCHMWGN 243

Query: 258 QPWNTGDLSRNNWLVALFTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVIGLA 317
           QPWNTGDLS+NNW+VAL TFGEGWHNNHHAFEYSARHG EWWQID+GWY I+FL VIGLA
Sbjct: 244 QPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGLEWWQIDYGWYFIRFLEVIGLA 303

Query: 318 TNVKLPSPSKKLAALDKSKFTLLKPMIN 344
           TNVK PS  +KLAAL+KSK T LKP IN
Sbjct: 304 TNVKFPSEKQKLAALNKSKPTFLKPTIN 331

[Close]

BLAST of Spg013857 vs. TAIR 10
Match: AT3G15850.1 (fatty acid desaturase 5 )

HSP 1 Score: 358.6 bits (919), Expect = 5.6e-99
Identity = 162/290 (55.86%), Postives = 205/290 (70.69%), Query Frame = 0


Query: 38  KSWTKMDKNVAFFFLILHLLCLLAPFDFSWSGFLLSVVIGNTTG-MAISVSFHRNLAHRS 97
           + W  MD       L +HLL LLAPF F+W    ++  +   TG + I++SFHRNL+H++
Sbjct: 96  REWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSFHRNLSHKA 155

Query: 98  FKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRYVDTERDPHSPIYGFWFSQITWFFD 157
           FKLPKWLEY  AYC + ALQG+PIDWVSTHR HH++ D++RDPHSP+ GFWFS + W FD
Sbjct: 156 FKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWFSHMNWMFD 215

Query: 158 SYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNVNDLERQAFYRFMHKTYMLHPLALS 217
           +  +T++                       G P+NV DLE+Q FYRF+  TY+LHPLAL+
Sbjct: 216 TNTITQRC----------------------GEPNNVGDLEKQPFYRFLRTTYILHPLALA 275

Query: 218 ALLYAIGGFHFILWGMCVRYIMSLHITSMINSVCHIWGKQPWNTGDLSRNNWLVALFTFG 277
             LYA+GGF FI+WGM VR +   HIT ++NS CH+WGKQ WNTGDLS+NNW VA   FG
Sbjct: 276 VALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSKNNWWVAALAFG 335

Query: 278 EGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVIGLATNVKLPSPSKK 327
           EGWHNNHHAFE+SARHG EWWQ+D  WY++KFL  IGLAT+VKLPS ++K
Sbjct: 336 EGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLPSEAQK 363

[Close]

BLAST of Spg013857 vs. TAIR 10
Match: AT1G06080.1 (delta 9 desaturase 1 )

HSP 1 Score: 327.0 bits (837), Expect = 1.8e-89
Identity = 152/314 (48.41%), Postives = 201/314 (64.01%), Query Frame = 0


Query: 19  ESKQEPKEMVKSPPRLYRRK------SWTKMDKNVAFFFLILHLLCLLAPFDFSWSGFLL 78
           E ++  K+M      + R+K       W ++D   AF  L +H LCLLAPF+F+W    +
Sbjct: 7   EKEENNKKMAADKAEMGRKKRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRV 66

Query: 79  SVVIGNTTGMAISVSFHRNLAHRSFKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRY 138
           ++++    G+ I+VS+HRNLAHRSFK+PKWLEY  AYC   A+QGDPIDWVSTHR HH++
Sbjct: 67  ALIVYTVGGLGITVSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQF 126

Query: 139 VDTERDPHSPIYGFWFSQITWFFDSYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNV 198
            D++RDPHSP  GFWFS + W FD+  L  K                       G   NV
Sbjct: 127 TDSDRDPHSPNEGFWFSHLLWLFDTGYLVEKC----------------------GRRTNV 186

Query: 199 NDLERQAFYRFMHKTYMLHPLALSALLYAIGGFHFILWGMCVRYIMSLHITSMINSVCHI 258
            DL+RQ +Y+F+ +T + H L    LLY  GG  F+ WGM +   M  H+T +INS+CH+
Sbjct: 187 EDLKRQWYYKFLQRTVLYHILTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCLINSLCHV 246

Query: 259 WGKQPWNTGDLSRNNWLVALFTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVI 318
           WG + W T D SRN W +++F+FGE WHNNHHAFE SAR G EWWQID  WYI++FL +I
Sbjct: 247 WGSRTWKTNDTSRNVWWLSVFSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFLEII 298

Query: 319 GLATNVKLPSPSKK 327
           GLAT+VKLPS S++
Sbjct: 307 GLATDVKLPSESQR 298

[Close]

BLAST of Spg013857 vs. TAIR 10
Match: AT3G15870.1 (Fatty acid desaturase family protein )

HSP 1 Score: 317.0 bits (811), Expect = 1.9e-86
Identity = 146/293 (49.83%), Postives = 190/293 (64.85%), Query Frame = 0


Query: 33  RLYRRKSWTKMDKNVAFFFLILHLLCLLAPFDFSWSGFLLSVVIGNTTGMAISVSFHRNL 92
           R +  +SWT  D +    F+  HLL LLAPF FSW  F +   +    G+ I++S+HRNL
Sbjct: 84  RSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGICITLSYHRNL 143

Query: 93  AHRSFKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRYVDTERDPHSPIYGFWFSQIT 152
           +HRSF LPKWLEY  AY    A QGDPI+WVS HR HH++ +T+RDPHSP  GFWFS + 
Sbjct: 144 SHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQGFWFSHMA 203

Query: 153 WFFDSYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNVNDLERQAFYRFMHKTYMLHP 212
           W FD+ ++                          G  +NV+DL RQ FYRF+ +T +LH 
Sbjct: 204 WIFDTSSILENC----------------------GGEENVDDLVRQPFYRFLQRTVLLHM 263

Query: 213 LALSALLYAIGGFHFILWGMCVRYIMSLHITSMINSVCHIWGKQPWNTGDLSRNNWLVAL 272
           +A S L Y  GG   ++WG+ +   + LH+T ++NSVCHIWG + WNT D S+NNW VA+
Sbjct: 264 MAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSDFSKNNWWVAI 323

Query: 273 FTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVIGLATNVKLPSPSK 326
            T GEGWHNNHHAFE+SARHG EWWQ+D  W +I+FL  IGLATNVKLP+ ++
Sbjct: 324 LTLGEGWHNNHHAFEFSARHGLEWWQLDITWCLIRFLEAIGLATNVKLPTETQ 354

[Close]

BLAST of Spg013857 vs. TAIR 10
Match: AT2G31360.1 (16:0delta9 desaturase 2 )

HSP 1 Score: 311.6 bits (797), Expect = 7.8e-85
Identity = 151/326 (46.32%), Postives = 205/326 (62.88%), Query Frame = 0


Query: 1   MKASTIAKKRRKKSPLMEESKQEPKEMVKSPPRLYRRKSWTKMDKNVAFFFLILHLLCLL 60
           M  ++  ++  +K+P    + +E K+       ++  + W ++D         +H L LL
Sbjct: 1   MSVTSTVEENHQKNPSTPAAVEEKKKR----RWVFWDRRWRRLDYVKFSASFTVHSLALL 60

Query: 61  APFDFSWSGFLLSVVIGNTTGMAISVSFHRNLAHRSFKLPKWLEYSLAYCASHALQGDPI 120
           APF F+WS   ++ +     G+ I+VS+HRNLAHRSFK+PKWLEY LAYCA  A+QGDPI
Sbjct: 61  APFYFTWSALWVTFLFYTIGGLGITVSYHRNLAHRSFKVPKWLEYLLAYCALLAIQGDPI 120

Query: 121 DWVSTHRCHHRYVDTERDPHSPIYGFWFSQITWFFDSYNLTRKVCPNYFNNFQKVERNSF 180
           DWVSTHR HH++ D+ERDPHSP  GFWFS + W +DS  L  K                 
Sbjct: 121 DWVSTHRYHHQFTDSERDPHSPKEGFWFSHLLWIYDSAYLVSKC---------------- 180

Query: 181 VLFLKHGYPDNVNDLERQAFYRFMHKTYMLHPLALSALLYAIGGFHFILWGMCVRYIMSL 240
                 G   NV DL+RQ FYRF+ KT + H L L   L+ +GG  F+ WGM V   + +
Sbjct: 181 ------GRRANVEDLKRQWFYRFLQKTVLFHILGLGFFLFYLGGMSFVTWGMGVGAALEV 240

Query: 241 HITSMINSVCHIWGKQPWNTGDLSRNNWLVALFTFGEGWHNNHHAFEYSARHGHEWWQID 300
           H+T +INS+CHIWG + W T D SRN W +++F+FGE WHNNHHAFE SAR G EWWQID
Sbjct: 241 HVTCLINSLCHIWGTRTWKTNDTSRNVWWLSVFSFGESWHNNHHAFESSARQGLEWWQID 300

Query: 301 FGWYIIKFLHVIGLATNVKLPSPSKK 327
             WYI++F  +IGLAT+VK+P+ +++
Sbjct: 301 ISWYIVRFFEIIGLATDVKVPTEAQR 300

[Close]

BLAST of Spg013857 vs. TAIR 10
Match: AT1G06120.1 (Fatty acid desaturase family protein )

HSP 1 Score: 305.8 bits (782), Expect = 4.3e-83
Identity = 147/309 (47.57%), Postives = 194/309 (62.78%), Query Frame = 0


Query: 18  EESKQEPKEMVKSPPRLYRRKSWTKMDKNVAFFFLILHLLCLLAPFDFSWSGFLLSVVIG 77
           ++      + V+   R +  + WT++D         +HLLCLLAPF+++W  F  + ++G
Sbjct: 6   KDDSSSQSKAVRKEKRAFLFRKWTRVDVMRVSAVGAVHLLCLLAPFNYTWEAFRFAAMVG 65

Query: 78  NTTGMAISVSFHRNLAHRSFKLPKWLEYSLAYCASHALQGDPIDWVSTHRCHHRYVDTER 137
            +T ++I+ S+HRNL HRSFKLPKWLEY  AY A  ALQG PIDWVSTHR HH++ D++R
Sbjct: 66  ISTNLSITFSYHRNLTHRSFKLPKWLEYPFAYSALFALQGHPIDWVSTHRFHHQFTDSDR 125

Query: 138 DPHSPIYGFWFSQITWFFDSYNLTRKVCPNYFNNFQKVERNSFVLFLKHGYPDNVNDLER 197
           DPHSPI GFWFS + W FD+ +  R+ C                     G  DNV DL++
Sbjct: 126 DPHSPIEGFWFSHVFWIFDT-SYIREKC---------------------GGRDNVMDLKQ 185

Query: 198 QAFYRFMHKTYMLHPLALSALLYAIGGFHFILWGMCVRYIMSLHITSMINSVCHIWGKQP 257
           Q FYRF+  T  LH L    L+Y  GG  ++ W + V   +  H T +INS CHIWG + 
Sbjct: 186 QWFYRFLQNTIGLHILTFWILVYLWGGLPYLTWSVGVGGAIGYHATWLINSACHIWGSRA 245

Query: 258 WNTGDLSRNNWLVALFTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLHVIGLATN 317
           WNT D SRN W +  FT GE WHNNHHAFE SARHG EW+Q+D  WY+I F  V+GLAT+
Sbjct: 246 WNTKDTSRNIWWLGPFTMGESWHNNHHAFEASARHGLEWYQVDLTWYLIWFFQVLGLATD 292

Query: 318 VKLPSPSKK 327
           VKLP+ ++K
Sbjct: 306 VKLPTDAQK 292

The following BLAST results are available for this feature:

BLAST of Spg013857 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038899717.1	1.8e-163	81.35	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [B...	[more]
XP_008451258.1	1.4e-160	80.12	PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplas...	[more]
XP_023549408.1	3.4e-159	80.32	delta-9 acyl-lipid desaturase 2-like [Cucurbita pepo subsp. pepo]	[more]
XP_011659770.1	1.3e-158	79.75	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic [Cucumi...	[more]
XP_022953867.1	2.2e-158	79.25	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [C...	[more]

BLAST of Spg013857 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q949X0	7.8e-98	55.86	Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arab...	[more]
O65797	2.5e-88	48.41	Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=...	[more]
Q9FV68	4.8e-87	53.51	Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii OX=28973 PE=1 SV=1	[more]
P0DOW2	6.2e-87	52.76	Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1	[more]
Q9LVZ3	2.6e-85	49.83	Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]

BLAST of Spg013857 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BS49	6.7e-161	80.12	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]
A0A0A0KAB1	2.8e-159	79.20	FA_desaturase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G3763...	[more]
A0A6J1GPG5	1.1e-158	79.25	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]
A0A6J1JUL6	1.2e-157	79.25	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]
A0A1S3BQG5	5.5e-155	76.22	palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS...	[more]

BLAST of Spg013857 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G15850.1	5.6e-99	55.86	fatty acid desaturase 5	[more]
AT1G06080.1	1.8e-89	48.41	delta 9 desaturase 1	[more]
AT3G15870.1	1.9e-86	49.83	Fatty acid desaturase family protein	[more]
AT2G31360.1	7.8e-85	46.32	16:0delta9 desaturase 2	[more]
AT1G06120.1	4.3e-83	47.57	Fatty acid desaturase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015876	Acyl-CoA desaturase	PRINTS	PR00075	FACDDSATRASE	coord: 89..109 score: 37.09 coord: 126..155 score: 46.32
IPR015876	Acyl-CoA desaturase	PANTHER	PTHR11351	ACYL-COA DESATURASE	coord: 32..328
IPR015876	Acyl-CoA desaturase	CDD	cd03505	Delta9-FADS-like	coord: 66..319 e-value: 5.08443E-71 score: 217.038
IPR005804	Fatty acid desaturase domain	PFAM	PF00487	FA_desaturase	coord: 66..303 e-value: 9.1E-16 score: 58.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..30
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 11..30
None	No IPR available	PANTHER	PTHR11351:SF87	FATTY ACID DESATURASE, TYPE 1, CORE-RELATED	coord: 32..328

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg013857.1	Spg013857.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006633	fatty acid biosynthetic process
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0009979	16:0 monogalactosyldiacylglycerol desaturase activity
molecular_function	GO:0016717	oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water