Spg013841 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg013841
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Thiamine pyrophosphokinase
Location	scaffold3: 44585218 .. 44588741 (+)
RNA-Seq Expression	Spg013841
Synteny	Spg013841

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATCGGTTTCTGGAAGTTCTTATACTTTGACCACCGAAAAAAGAAAAGAAAAAATCGATTTGCCAATCGAATCGAATCTTCATTCACAAAAATTTTCAATCGCTCTCATTTTCGCCATGGGCGTCATGTTCCACTCTTCAGACTTTCTTCTCCCTCCTCCTCCTCCTCCTCCTCCTTCTTCTTCTCCGGCCGACAGCCGCCGTTCTTCAACCTACGCCCTTGTCCTGCTCAATCAACGCCTCCCCAAATTCACTCCCCTGCTCTGGAAGCACGGTTAATCTACGAATCCCTTTTCACTCTGTACTTTTTTGGTTCTGGGTTTTGATTTTTGAGGGGTTTTGTTCGAGGGTTTTGATGAACTTTTACGTTTTGTGTTGATGTGTTGTTATGTGTGTTGTTGTAGAGCAGCACAACTTCGTATTTGTGCCGATGGAGGCGCCAATCGCGTATTCGATGAATTGCCTTTGCTGCTTCCTCATCTCGACGCTTTGGATGTTCGTAATAGGTTTGTTTCGGTGTCCTTTTCCTCTTTTTCGTTTTAGTATGCATTTGAACCATTATTAGTGTTTTTTTGTTCTTTCTTCTTGTTTTGATTTAGCTTCTCTCTTTAGTAATGGAGTTTCGTGCCTGGGGGGTTTTGTTCTCTGTTTGTTTTGGAAATTTTAGATGGTCAATGCGTGAAAACATAAACCTTCAAAAGAAGGAAAAAGAAAAAGAAAAAGAAAATCTTTTAAGGGAGTGATTATTGATGTCATGGACCACGCCCTGGATGGTACTTTTCAGTTTATTTACTATCGATTGTCAAACTTTTAGGGTTTATTTGTTTTTGGTTAGATTACTATTTTAATTTGGTACTTGTGGTTCTTTTCTCCAATACGTTTCTTTCATTATCAAATGTTAACAGTTTGTTATTAAGTTATATGTATGTTTTCTTTGGATACCAACTCATCGCCTCCTTGGGAAGTACGTTGAGTAAATGTTCTTTGAAAGGTGTTTTTCTTATTTGGGTTTCTTTTTTTTTTCACTGTTATTGAAATTTTTCTTTGATAATCATGGACTTGTGGTTAGTGATCTTATGTGTTAAGTAATCTCAATTTGTACAAATCACACAATCACCATTCTTAAGAATAATCTTCTTTCCGAAAAGAATGAAAAAGAAAGTCCTAAATATTCCCCAAGTTTCAAGATGCGATTGACACTAAAAAAACAAATTCTAGTGGCTGCCTGAATAAGAAATTTAAAAATATTTTGTTGGTATTTGATGTCTATGTATCATTTTCGAGCATTTCATGATAGTTGATATTATTGTTGTCAAATGAATAAACTATTCTAGAATTAGATATCTTATGTTTTGCATTTCATGAATGTTGATATTATTGTTGTCAAATGAATAAACTATTCTAGAATTAGATATCTTATGTTTTGGTGACCTTGACTGTACTTTGATCCTATCTAATGATTTTTTTTTGTAATTCCAGCTATAAGCCAGATGTTATCAGAGGAGACATGGATTCAATTCGGACAGAAGTACTAGATTTTTATGCTAAGCAAGTACGTTATTCTGCCCTTCTCTCTGTCCAAAGAATTTAGCTTTTGCTATCTATTTTGAGACCTTGCATAGTTCTGATTGCTCTTTCTGCATCTTGTGTTTAGTTTTGCAATTTTTATGTGTTGATGAAATTCACTTGCATTACATGTTGTATTTACTATTTTGTTATCATGTGTATATTGCTGGCTCAAGATATCCATATTTACATGGTGCAGGGAACCAAGATATTTGATGAATCTGAAGATCAGGATACCACAGACCTACATAAATGTGTTGCATATATTCTTCAGTCTATGCCAAATGAGGAGGGTTCTAATGTAGGTTGGATCATGATGTAAATTTTAAGTGGCTTGTTATTTAAGATAGAACTTCATAATCTTAAAAAAGAAAAAAGAAAAAAGAAAAAAAGGCTAGGACTTTATTTGGCTGTTCACATTCAATAATGTTGAGTTGTTCATTTTCAGCTGTGCATACTTGTTGCTGGAGCTCTAGGCGGACGGTTTGATCATGAAATAGGAAACATCAATGTTCTATGTCGTTTCTCAACCACGCATATAATCCTTCTGTCCGATGATTGCCTTATTCATCTCCTGCCAAGCACTCATCACCATGAAATTCATGTTCACTCATCTGTTGAGGGTCCACATTGTGGTCTTGTTCCAATTGGGATGCCCTCTGGAAGCACTACAACCACTGGTCTCCAATGGGATTTGAGTAAGTACTGTCAATATCATCCCACCTTCTGGATCTAATGCTCTGTTTATTCTCAATGAATGGCCGATCGTGCTATGTCGTATTGTTCTTATGTGCATCCTATGCTGTTCATGCCTTTGGTTTCAAGCAATTTTGAGATGCAAAATGAGCTGACAACGTGATCCATCCGTTTTATTCCTCTTGAGTTCAAATCTTAACTTCACTTTTCATAAAACCTTCATTTGGTACATGCATAGAATAGTTCTATTCAGCAGCTTTATGCTCAATTTCTTTAGCTTCAAATTCTTATCTTATGATACTTTTAGACAAGGTCTAAAAGGATGTTTGGGACTCCATTTTCTTTTTCAATTGGAACTCTGCTTTTCTGCTCTGTTTTAACTTCAACTTGGAAGCTTTCTTGTAATAGAATGCTTAGTTAATTCTAATGCCTGCTCTGTTAACTTTTGGTTTTAATGTCAATTCCAGCTGACACAGAGATGAAGTTTGGGGGTCTAATTAGCACATCAAATATAGTTAAAGGAGAGAAAGTAACAGTGCAATCTGACTCCGATCTTCTCTGGACGATATCGTTAAAAAGCAATGAGATAACGCATTCTTGCAGCACGTAAAGCCAAATTTAGCAATGCTTCTTTTCATTTTCAGAATTAGGTAATGGAATCTTCTTTCTCCACTTAAAGTAATAAACATTTTGAAAGATCTACTCCTTGATCTTAGTCCTTTTCATAGCATTTGATTGATTGATTATCAATTAAATTCATTTTTATGTGATTAGGAAGTTGTATCTGCAATGGCTAAACTCTCTCTCTCTCTCTCTCTCTCTGATTCTGGAAGTCAGTGCCATTATATGTATGTCATTTGTATTTTGTAACACTTAAGTACTCTATATATTTCATTGAGAGCTGAAGCATATTTTATCAGCAACATTTTTATTCCTATGGTTTTAGAAGTATAATGTTTGGTTTTATTCAAGTCGTATTCATGAGTTTGTAACTGGAATGTATCTGAATTTGTACTATGATAAGTTCATTTGTTCCTTCACTTAATCCAATTACATATATAATATGCTTTCTGATTAAATAAAGATTGAGCCCTAAAATAAGTAGCTTTCTTGCTATCACCTTAATTGCCTCTTATTTGCTCTTGCTACATGCCAACTTTGGAGAGTACTTAATGCTATCAAGTTGAGCGTATATTATAGTGACTTAAAAATTTTCATTGCTAGGTCCCTAATGATATTGTTTAAATGTATTTTAATCCATGTG

mRNA sequence

ATGGGCGTCATGTTCCACTCTTCAGACTTTCTTCTCCCTCCTCCTCCTCCTCCTCCTCCTTCTTCTTCTCCGGCCGACAGCCGCCGTTCTTCAACCTACGCCCTTGTCCTGCTCAATCAACGCCTCCCCAAATTCACTCCCCTGCTCTGGAAGCACGCAGCACAACTTCGTATTTGTGCCGATGGAGGCGCCAATCGCGTATTCGATGAATTGCCTTTGCTGCTTCCTCATCTCGACGCTTTGGATGTTCGTAATAGCTATAAGCCAGATGTTATCAGAGGAGACATGGATTCAATTCGGACAGAAGTACTAGATTTTTATGCTAAGCAAGGAACCAAGATATTTGATGAATCTGAAGATCAGGATACCACAGACCTACATAAATGTGTTGCATATATTCTTCAGTCTATGCCAAATGAGGAGGGTTCTAATCTGTGCATACTTGTTGCTGGAGCTCTAGGCGGACGGTTTGATCATGAAATAGGAAACATCAATGTTCTATGTCGTTTCTCAACCACGCATATAATCCTTCTGTCCGATGATTGCCTTATTCATCTCCTGCCAAGCACTCATCACCATGAAATTCATGTTCACTCATCTGTTGAGGGTCCACATTGTGGTCTTGTTCCAATTGGGATGCCCTCTGGAAGCACTACAACCACTGGTCTCCAATGGGATTTGACTGACACAGAGATGAAGTTTGGGGGTCTAATTAGCACATCAAATATAGTTAAAGGAGAGAAAGTAACAGTGCAATCTGACTCCGATCTTCTCTGGACGATATCGTTAAAAAGCAATGAGATAACGCATTCTTGCAGCACGTAA

Coding sequence (CDS)

ATGGGCGTCATGTTCCACTCTTCAGACTTTCTTCTCCCTCCTCCTCCTCCTCCTCCTCCTTCTTCTTCTCCGGCCGACAGCCGCCGTTCTTCAACCTACGCCCTTGTCCTGCTCAATCAACGCCTCCCCAAATTCACTCCCCTGCTCTGGAAGCACGCAGCACAACTTCGTATTTGTGCCGATGGAGGCGCCAATCGCGTATTCGATGAATTGCCTTTGCTGCTTCCTCATCTCGACGCTTTGGATGTTCGTAATAGCTATAAGCCAGATGTTATCAGAGGAGACATGGATTCAATTCGGACAGAAGTACTAGATTTTTATGCTAAGCAAGGAACCAAGATATTTGATGAATCTGAAGATCAGGATACCACAGACCTACATAAATGTGTTGCATATATTCTTCAGTCTATGCCAAATGAGGAGGGTTCTAATCTGTGCATACTTGTTGCTGGAGCTCTAGGCGGACGGTTTGATCATGAAATAGGAAACATCAATGTTCTATGTCGTTTCTCAACCACGCATATAATCCTTCTGTCCGATGATTGCCTTATTCATCTCCTGCCAAGCACTCATCACCATGAAATTCATGTTCACTCATCTGTTGAGGGTCCACATTGTGGTCTTGTTCCAATTGGGATGCCCTCTGGAAGCACTACAACCACTGGTCTCCAATGGGATTTGACTGACACAGAGATGAAGTTTGGGGGTCTAATTAGCACATCAAATATAGTTAAAGGAGAGAAAGTAACAGTGCAATCTGACTCCGATCTTCTCTGGACGATATCGTTAAAAAGCAATGAGATAACGCATTCTTGCAGCACGTAA

Protein sequence

MGVMFHSSDFLLPPPPPPPPSSSPADSRRSSTYALVLLNQRLPKFTPLLWKHAAQLRICADGGANRVFDELPLLLPHLDALDVRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESEDQDTTDLHKCVAYILQSMPNEEGSNLCILVAGALGGRFDHEIGNINVLCRFSTTHIILLSDDCLIHLLPSTHHHEIHVHSSVEGPHCGLVPIGMPSGSTTTTGLQWDLTDTEMKFGGLISTSNIVKGEKVTVQSDSDLLWTISLKSNEITHSCST

Homology

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BLAST of Spg013841 vs. NCBI nr
Match: KAG7030224.1 (Thiamine pyrophosphokinase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 483.0 bits (1242), Expect = 1.7e-132
Identity = 238/271 (87.82%), Postives = 247/271 (91.14%), Query Frame = 0


Query: 1   MGVMFHSSDFLLPPPPPPPPSSSPADSRRSSTYALVLLNQRLPKFTPLLWKHAAQLRICA 60
           MGV FHSSDFLL     P  +SSPA    SSTYALVLLNQ LPKFTPLLWKH A+LR+CA
Sbjct: 1   MGVFFHSSDFLL-----PSSTSSPAGHPPSSTYALVLLNQSLPKFTPLLWKH-AKLRLCA 60

Query: 61  DGGANRVFDELPLLLPHLDALDVRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESED 120
           DGGANRVFDELPLL PH DA D+RNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESED
Sbjct: 61  DGGANRVFDELPLLFPHQDATDIRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESED 120

Query: 121 QDTTDLHKCVAYILQSMPNEEGSNLCILVAGALGGRFDHEIGNINVLCRFSTTHIILLSD 180
           QDTTDLHKCVAYILQSMPN+E SNLCILVAGALGGRFDHE+GNINVLCRFSTT IILLSD
Sbjct: 121 QDTTDLHKCVAYILQSMPNQEESNLCILVAGALGGRFDHEMGNINVLCRFSTTRIILLSD 180

Query: 181 DCLIHLLPSTHHHEIHVHSSVEGPHCGLVPIGMPSGSTTTTGLQWDLTDTEMKFGGLIST 240
           DCLIHLLP THHHEIHVHSSVEGPHCGL+PIGMPSG TTTTGLQWDLTDTEMKFGGL+ST
Sbjct: 181 DCLIHLLPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDTEMKFGGLVST 240

Query: 241 SNIVKGEKVTVQSDSDLLWTISLKSNEITHS 272
           SNIVKGEKVTVQSDSDLLWT+SLK NE  HS
Sbjct: 241 SNIVKGEKVTVQSDSDLLWTLSLKGNETRHS 265

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BLAST of Spg013841 vs. NCBI nr
Match: XP_023515266.1 (thiamine pyrophosphokinase 1 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 481.9 bits (1239), Expect = 3.7e-132
Identity = 237/271 (87.45%), Postives = 248/271 (91.51%), Query Frame = 0


Query: 1   MGVMFHSSDFLLPPPPPPPPSSSPADSRRSSTYALVLLNQRLPKFTPLLWKHAAQLRICA 60
           MGV FHSSDFLL     P  +SSPA    SSTYALVLLNQ LPKFTPLLWKH A+LR+CA
Sbjct: 1   MGVFFHSSDFLL-----PSSTSSPAGHPPSSTYALVLLNQSLPKFTPLLWKH-AKLRLCA 60

Query: 61  DGGANRVFDELPLLLPHLDALDVRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESED 120
           DGGANRVFDELPLLLPH DA D+RNSYKPDVIRGDMDSIRTEVL+FYAKQGTKIFDESED
Sbjct: 61  DGGANRVFDELPLLLPHQDATDIRNSYKPDVIRGDMDSIRTEVLEFYAKQGTKIFDESED 120

Query: 121 QDTTDLHKCVAYILQSMPNEEGSNLCILVAGALGGRFDHEIGNINVLCRFSTTHIILLSD 180
           QDTTDLHKCVAYILQSMPN+E SNLCILVAGALGGRFDHE+GN+NVLCRFS+T IILLSD
Sbjct: 121 QDTTDLHKCVAYILQSMPNQEESNLCILVAGALGGRFDHEMGNVNVLCRFSSTRIILLSD 180

Query: 181 DCLIHLLPSTHHHEIHVHSSVEGPHCGLVPIGMPSGSTTTTGLQWDLTDTEMKFGGLIST 240
           DCLIHLLP THHHEIHVHSSVEGPHCGL+PIGMPSG TTTTGLQWDLTDTEMKFGGL+ST
Sbjct: 181 DCLIHLLPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDTEMKFGGLVST 240

Query: 241 SNIVKGEKVTVQSDSDLLWTISLKSNEITHS 272
           SNIVKGEKVTVQSDSDLLWTISLK NE  HS
Sbjct: 241 SNIVKGEKVTVQSDSDLLWTISLKGNETRHS 265

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BLAST of Spg013841 vs. NCBI nr
Match: XP_023004625.1 (thiamine pyrophosphokinase 1 isoform X1 [Cucurbita maxima])

HSP 1 Score: 479.6 bits (1233), Expect = 1.9e-131
Identity = 236/267 (88.39%), Postives = 246/267 (92.13%), Query Frame = 0


Query: 1   MGVMFHSSDFLLPPPPPPPPSSSPADSRRSSTYALVLLNQRLPKFTPLLWKHAAQLRICA 60
           MGV FHSSDFLL     P  +SSPA    SSTYALVLLNQRLPKFTPLLWKH A+LR+CA
Sbjct: 1   MGVFFHSSDFLL-----PSSTSSPAGHPPSSTYALVLLNQRLPKFTPLLWKH-AKLRLCA 60

Query: 61  DGGANRVFDELPLLLPHLDALDVRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESED 120
           DGGANRVFDELPLL PH DA D+RNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESED
Sbjct: 61  DGGANRVFDELPLLFPHQDATDIRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESED 120

Query: 121 QDTTDLHKCVAYILQSMPNEEGSNLCILVAGALGGRFDHEIGNINVLCRFSTTHIILLSD 180
           QDTTDLHKCVAYILQSMPN+E SNLCILVAGALGGRFDHE+GNINVLCRFS+T IILLSD
Sbjct: 121 QDTTDLHKCVAYILQSMPNQEESNLCILVAGALGGRFDHEMGNINVLCRFSSTRIILLSD 180

Query: 181 DCLIHLLPSTHHHEIHVHSSVEGPHCGLVPIGMPSGSTTTTGLQWDLTDTEMKFGGLIST 240
           DCLIHLLP THHHEIHVHSSVEGPHCGL+PIGMPSG TTTTGLQWDLTD+EMKFGGL+ST
Sbjct: 181 DCLIHLLPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDSEMKFGGLVST 240

Query: 241 SNIVKGEKVTVQSDSDLLWTISLKSNE 268
           SNIVKGEKVTVQSDSDLLWTISLK NE
Sbjct: 241 SNIVKGEKVTVQSDSDLLWTISLKGNE 261

[Close]

BLAST of Spg013841 vs. NCBI nr
Match: KAG6593191.1 (Thiamine pyrophosphokinase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 478.4 bits (1230), Expect = 4.1e-131
Identity = 238/272 (87.50%), Postives = 248/272 (91.18%), Query Frame = 0


Query: 1   MGVMFHSSDFLLPPPPPPPPSSSPADSRRSSTYALVLLNQRLPKFTPLLWKHAAQLRICA 60
           MGV FHSSDFLL     P  +SSPA    SSTYALVLLNQ LPKFTPLLWKH A+LR+CA
Sbjct: 1   MGVFFHSSDFLL-----PSSTSSPAGHPPSSTYALVLLNQSLPKFTPLLWKH-AKLRLCA 60

Query: 61  DGGANRVFDELPLLLPHLDALDVRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESED 120
           DGGANRVFDELPLL PH DA D+RNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESED
Sbjct: 61  DGGANRVFDELPLLFPHQDATDIRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESED 120

Query: 121 QDTTDLHKCVAYILQSMPNEEGSNLCILVAGALGGRFDHEIGNINVLCRFSTTHIILLSD 180
           QDTTDLHKCVAYILQSMPN+E SNLCILVAGALGGRFDHE+GNINVLCRFSTT IILLSD
Sbjct: 121 QDTTDLHKCVAYILQSMPNQEESNLCILVAGALGGRFDHEMGNINVLCRFSTTRIILLSD 180

Query: 181 DCLIHLLPSTHHHEIHVHSSVEGPHCGLVPIGMPSGSTTTTGLQWDLTDTEMKFGGLIST 240
           DCLIHLLP THHHEIHVHSSVEGPHCGL+PIGMPSG TTTTGLQWDLTDTEMKFGGL+ST
Sbjct: 181 DCLIHLLPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDTEMKFGGLVST 240

Query: 241 SNIVKGEKVTVQSDSDLLWTISLK-SNEITHS 272
           SNIV+GEKVTVQSDSDLLWT+SLK SNE  HS
Sbjct: 241 SNIVEGEKVTVQSDSDLLWTLSLKGSNETRHS 266

[Close]

BLAST of Spg013841 vs. NCBI nr
Match: XP_008451592.1 (PREDICTED: thiamine pyrophosphokinase 1 isoform X1 [Cucumis melo])

HSP 1 Score: 477.6 bits (1228), Expect = 7.0e-131
Identity = 238/265 (89.81%), Postives = 249/265 (93.96%), Query Frame = 0


Query: 1   MGVMFHSSDFLLPPPPPPPPSSSPADSRRSSTYALVLLNQRLPKFTPLLWKHAAQLRICA 60
           M V+FHSSDFLL     P PSSSP + RRSSTYALVLLNQRLPKFTPLLWKH AQLR+CA
Sbjct: 1   MEVLFHSSDFLL-----PSPSSSPPNHRRSSTYALVLLNQRLPKFTPLLWKH-AQLRLCA 60

Query: 61  DGGANRVFDELPLLLPHLDALDVRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESED 120
           DGGANRVFD+LPL+ PHLDALDVRNSYKPDVIRGDMDSIRTEVL+FYAKQGTKIFDESED
Sbjct: 61  DGGANRVFDDLPLMFPHLDALDVRNSYKPDVIRGDMDSIRTEVLEFYAKQGTKIFDESED 120

Query: 121 QDTTDLHKCVAYILQSMPNEEGSNLCILVAGALGGRFDHEIGNINVLCRFSTTHIILLSD 180
           QDTTDLHKCVAYILQS+PN+E SNLCILVAGALGGRFDHEIGNINVLCRFSTT IILLSD
Sbjct: 121 QDTTDLHKCVAYILQSIPNQE-SNLCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSD 180

Query: 181 DCLIHLLPSTHHHEIHVHSSVEGPHCGLVPIGMPSGSTTTTGLQWDLTDTEMKFGGLIST 240
           DCLIHLLP THHHEI +HSSVEGPHCGL+PIGMPSGSTTTTGLQWDL+DTEMKFGGLIST
Sbjct: 181 DCLIHLLPRTHHHEILIHSSVEGPHCGLIPIGMPSGSTTTTGLQWDLSDTEMKFGGLIST 240

Query: 241 SNIVKGEKVTVQSDSDLLWTISLKS 266
           SNIVK EKVTVQSDSDLLWTISLKS
Sbjct: 241 SNIVKEEKVTVQSDSDLLWTISLKS 258

[Close]

BLAST of Spg013841 vs. ExPASy Swiss-Prot
Match: F4IV16 (Thiamine pyrophosphokinase 2 OS=Arabidopsis thaliana OX=3702 GN=TPK2 PE=2 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 3.0e-100
Identity = 175/264 (66.29%), Postives = 207/264 (78.41%), Query Frame = 0


Query: 1   MGVMFHSSDFLLPPPPPPPPSSSPADSRRSSTYALVLLNQRLPKFTPLLWKHAAQLRICA 60
           M VM HSS FLL           P D    + YALV+LNQ LP+FTPLLW+H A+LR+CA
Sbjct: 5   MDVMIHSSSFLL-----------PCDETCGTRYALVVLNQNLPRFTPLLWEH-AKLRLCA 64

Query: 61  DGGANRVFDELPLLLPHLDALDVRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESED 120
           DGGANR++DELPL  PH D   +RN YKPDVI+GDMDSIR +VLDFY   GTK+ DES D
Sbjct: 65  DGGANRIYDELPLFFPHEDPFVIRNRYKPDVIKGDMDSIRRDVLDFYVYWGTKVIDESHD 124

Query: 121 QDTTDLHKCVAYILQSMPNEEGSNLCILVAGALGGRFDHEIGNINVLCRFSTTHIILLSD 180
           QDTTDL KC++YI  S  N+E S L IL  GALGGRFDHE GN+NVL R+  T I+LLSD
Sbjct: 125 QDTTDLDKCISYIRHSTLNQESSRLQILATGALGGRFDHEAGNLNVLYRYPDTRIVLLSD 184

Query: 181 DCLIHLLPSTHHHEIHVHSSVEGPHCGLVPIGMPSGSTTTTGLQWDLTDTEMKFGGLIST 240
           DCLI LLP TH HEIH+HSS++GPHCGL+PIG PS +TTT+GL+WDL++TEM+FGGLIST
Sbjct: 185 DCLIQLLPKTHRHEIHIHSSLQGPHCGLIPIGTPSANTTTSGLKWDLSNTEMRFGGLIST 244

Query: 241 SNIVKGEKVTVQSDSDLLWTISLK 265
           SN+VK E +TV+SDSDLLWTIS+K
Sbjct: 245 SNLVKEEIITVESDSDLLWTISIK 256

[Close]

BLAST of Spg013841 vs. ExPASy Swiss-Prot
Match: B9DGU7 (Thiamine pyrophosphokinase 1 OS=Arabidopsis thaliana OX=3702 GN=TPK1 PE=2 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 6.7e-100
Identity = 177/269 (65.80%), Postives = 210/269 (78.07%), Query Frame = 0


Query: 1   MGVMFHSSDFLLPPPPPPPPSSSPADSRRSSTYALVLLNQRLPKFTPLLWKHAAQLRICA 60
           M VM HSS FLLP            ++   + YALV+LNQ LP+FTPLLW+H A+LR+CA
Sbjct: 4   MDVMIHSSSFLLPCD----------ETSTGTRYALVVLNQSLPRFTPLLWEH-AKLRLCA 63

Query: 61  DGGANRVFDELPLLLPHLDALDVRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESED 120
           DGGANR++DELPL  P+ DAL +RN YKPDVI+GDMDSIR +VLDFY   GTK+ DES D
Sbjct: 64  DGGANRIYDELPLFFPNEDALAIRNRYKPDVIKGDMDSIRRDVLDFYINLGTKVIDESHD 123

Query: 121 QDTTDLHKCVAYILQSMPNEEGSNLCILVAGALGGRFDHEIGNINVLCRFSTTHIILLSD 180
           QDTTDL KC+ YI  S  N+E S L IL  GALGGRFDHE GN+NVL R+  T I+LLSD
Sbjct: 124 QDTTDLDKCILYIRHSTLNQETSGLQILATGALGGRFDHEAGNLNVLYRYPDTRIVLLSD 183

Query: 181 DCLIHLLPSTHHHEIHVHSSVEGPHCGLVPIGMPSGSTTTTGLQWDLTDTEMKFGGLIST 240
           DCLI LLP TH HEIH+ SS+EGPHCGL+PIG PS  TTT+GLQWDL++TEM+FGGLIST
Sbjct: 184 DCLIQLLPKTHRHEIHIQSSLEGPHCGLIPIGTPSAKTTTSGLQWDLSNTEMRFGGLIST 243

Query: 241 SNIVKGEKVTVQSDSDLLWTISLKSNEIT 270
           SN+VK EK+TV+SDSDLLWTIS+K   ++
Sbjct: 244 SNLVKEEKITVESDSDLLWTISIKKTGLS 261

[Close]

BLAST of Spg013841 vs. ExPASy Swiss-Prot
Match: Q5JK24 (Thiamine pyrophosphokinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=TPK1 PE=2 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 9.7e-91
Identity = 171/263 (65.02%), Postives = 202/263 (76.81%), Query Frame = 0


Query: 4   MFHSSDFL-LPPPPPPPPSSSPADSRRSSTYALVLLNQRLPKFTPLLWKHAAQLRICADG 63
           M HSS FL LP    P P   PAD   S+ YA+V+LNQRLP+F PLLW   A+LR+CADG
Sbjct: 6   MTHSSSFLRLPATSSPHP--PPADD-ASAAYAVVVLNQRLPRFAPLLWDR-ARLRVCADG 65

Query: 64  GANRVFDELPLLLPHLDALDVRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESEDQD 123
           GANRVFD +P LLP  D   VR  YKPDVI+GDMDSIR EV ++Y+  G +I DES DQD
Sbjct: 66  GANRVFDGMPELLPAEDPDQVRMRYKPDVIKGDMDSIRPEVKEYYSNLGAEIVDESHDQD 125

Query: 124 TTDLHKCVAYILQSMPNEEGSNLCILVAGALGGRFDHEIGNINVLCRFSTTHIILLSDDC 183
           TTDLHKCV++I ++ P  E SNL ILV GALGGRFDHE+GNINVL RFS   I+LLSDDC
Sbjct: 126 TTDLHKCVSFITRNPPGSEESNLYILVLGALGGRFDHEMGNINVLYRFSNIRIVLLSDDC 185

Query: 184 LIHLLPSTHHHEIHVHSSVEGPHCGLVPIGMPSGSTTTTGLQWDLTDTEMKFGGLISTSN 243
            I LLP TH HEIH+  S+EGPHCGL+P+G PS STTTTGL+W+L +T M +GGLISTSN
Sbjct: 186 SIFLLPKTHSHEIHIERSIEGPHCGLIPMGSPSASTTTTGLRWNLDNTSMSYGGLISTSN 245

Query: 244 IVKGEKVTVQSDSDLLWTISLKS 266
           IV+ E V + SDSDL+WTISL++
Sbjct: 246 IVEEETVRITSDSDLIWTISLRN 264

[Close]

BLAST of Spg013841 vs. ExPASy Swiss-Prot
Match: Q60DX1 (Thiamine pyrophosphokinase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=TPK3 PE=2 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 1.5e-88
Identity = 165/263 (62.74%), Postives = 198/263 (75.29%), Query Frame = 0


Query: 4   MFHSSDFLLPPPPPPPPSSSPADSRRSSTYALVLLNQRLPKFTPLLWKHAAQLRICADGG 63
           M HSS FLL   P P  +++ AD+   + YAL++LNQRLP+F P LW   AQ+R+CADGG
Sbjct: 7   MSHSSAFLL---PSPSAAAAGADA-DGAAYALLVLNQRLPRFAPRLWDR-AQVRVCADGG 66

Query: 64  ANRVFDELPLLLPHLDALDVRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESEDQDT 123
           ANRVFD +P L P  D  +VR  YKPDVI+GD+DS+R EV ++Y+  GT+I DES DQDT
Sbjct: 67  ANRVFDGMPELFPGQDPDEVRRRYKPDVIKGDLDSVRPEVKEYYSNMGTQIVDESHDQDT 126

Query: 124 TDLHKCVAYILQSMPNEEGSNLCILVAGALGGRFDHEIGNINVLCRFSTTHIILLSDDCL 183
           TDLHKCVA+I ++      SNLCI   GALGGRFDHE+GNINVL  F    IILLSDDCL
Sbjct: 127 TDLHKCVAFITENSAIPNKSNLCIFALGALGGRFDHEMGNINVLHLFPNNRIILLSDDCL 186

Query: 184 IHLLPSTHHHEIHVHSSVEGPHCGLVPIGMPSGSTTTTGLQWDLTDTEMKFGGLISTSNI 243
           I LLP TH H IH+  S+EGPHCGL+PIG PS +TTTTGLQW+L +T M FGGLISTSNI
Sbjct: 187 IFLLPRTHTHNIHIERSIEGPHCGLIPIGAPSATTTTTGLQWNLDNTSMSFGGLISTSNI 246

Query: 244 VKGEK--VTVQSDSDLLWTISLK 265
           V+ E   VT+ SDSDL+WTISL+
Sbjct: 247 VREESTVVTITSDSDLIWTISLR 264

[Close]

BLAST of Spg013841 vs. ExPASy Swiss-Prot
Match: Q0JMW0 (Thiamine pyrophosphokinase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=TPK2 PE=2 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 2.0e-88
Identity = 164/265 (61.89%), Postives = 200/265 (75.47%), Query Frame = 0


Query: 1   MGVMFHSSDFLLPPPPPPPPSSSPADSRRSSTYALVLLNQRLPKFTPLLWKHAAQLRICA 60
           M VM HSS FLL  P    P++SPA       YALV+LNQ LP+F P LW H A+LRICA
Sbjct: 9   MEVMVHSSTFLL--PKLHQPANSPA-----KKYALVVLNQNLPRFVPRLWTH-AKLRICA 68

Query: 61  DGGANRVFDELPLLLPHLDALDVRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESED 120
           DGGANR+FDE+  +    D    R  Y P++I GDMDSIR EV  FY+ QG+KI D+S +
Sbjct: 69  DGGANRIFDEMFQMTNDPDYESTRKRYIPEIIEGDMDSIRPEVKQFYSSQGSKISDKSHN 128

Query: 121 QDTTDLHKCVAYILQSMPNEEGSNLCILVAGALGGRFDHEIGNINVLCRFSTTHIILLSD 180
           Q+TTDLHKC++ I +  P+ E +NLC+LV GALGGRFDHE  NIN+L  FS   I+LLSD
Sbjct: 129 QETTDLHKCISRIHRCTPDHEKTNLCVLVTGALGGRFDHEAANINILYLFSDMRIVLLSD 188

Query: 181 DCLIHLLPSTHHHEIHVHSSVEGPHCGLVPIGMPSGSTTTTGLQWDLTDTEMKFGGLIST 240
           DCLI LLP TH HEI++ SSVEGPHCGL P+G PSGSTTTTGL+W+L++ +M+FG +IST
Sbjct: 189 DCLIRLLPKTHKHEIYIESSVEGPHCGLFPVGAPSGSTTTTGLKWNLSEAKMRFGSMIST 248

Query: 241 SNIVKGEKVTVQSDSDLLWTISLKS 266
           SNIV  EKVTVQSD+DLLWTISL++
Sbjct: 249 SNIVHAEKVTVQSDADLLWTISLRN 265

[Close]

BLAST of Spg013841 vs. ExPASy TrEMBL
Match: A0A6J1KV43 (Thiamine pyrophosphokinase OS=Cucurbita maxima OX=3661 GN=LOC111497866 PE=3 SV=1)

HSP 1 Score: 479.6 bits (1233), Expect = 9.0e-132
Identity = 236/267 (88.39%), Postives = 246/267 (92.13%), Query Frame = 0


Query: 1   MGVMFHSSDFLLPPPPPPPPSSSPADSRRSSTYALVLLNQRLPKFTPLLWKHAAQLRICA 60
           MGV FHSSDFLL     P  +SSPA    SSTYALVLLNQRLPKFTPLLWKH A+LR+CA
Sbjct: 1   MGVFFHSSDFLL-----PSSTSSPAGHPPSSTYALVLLNQRLPKFTPLLWKH-AKLRLCA 60

Query: 61  DGGANRVFDELPLLLPHLDALDVRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESED 120
           DGGANRVFDELPLL PH DA D+RNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESED
Sbjct: 61  DGGANRVFDELPLLFPHQDATDIRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESED 120

Query: 121 QDTTDLHKCVAYILQSMPNEEGSNLCILVAGALGGRFDHEIGNINVLCRFSTTHIILLSD 180
           QDTTDLHKCVAYILQSMPN+E SNLCILVAGALGGRFDHE+GNINVLCRFS+T IILLSD
Sbjct: 121 QDTTDLHKCVAYILQSMPNQEESNLCILVAGALGGRFDHEMGNINVLCRFSSTRIILLSD 180

Query: 181 DCLIHLLPSTHHHEIHVHSSVEGPHCGLVPIGMPSGSTTTTGLQWDLTDTEMKFGGLIST 240
           DCLIHLLP THHHEIHVHSSVEGPHCGL+PIGMPSG TTTTGLQWDLTD+EMKFGGL+ST
Sbjct: 181 DCLIHLLPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDSEMKFGGLVST 240

Query: 241 SNIVKGEKVTVQSDSDLLWTISLKSNE 268
           SNIVKGEKVTVQSDSDLLWTISLK NE
Sbjct: 241 SNIVKGEKVTVQSDSDLLWTISLKGNE 261

[Close]

BLAST of Spg013841 vs. ExPASy TrEMBL
Match: A0A1S3BSZ4 (Thiamine pyrophosphokinase OS=Cucumis melo OX=3656 GN=LOC103492826 PE=3 SV=1)

HSP 1 Score: 477.6 bits (1228), Expect = 3.4e-131
Identity = 238/265 (89.81%), Postives = 249/265 (93.96%), Query Frame = 0


Query: 1   MGVMFHSSDFLLPPPPPPPPSSSPADSRRSSTYALVLLNQRLPKFTPLLWKHAAQLRICA 60
           M V+FHSSDFLL     P PSSSP + RRSSTYALVLLNQRLPKFTPLLWKH AQLR+CA
Sbjct: 1   MEVLFHSSDFLL-----PSPSSSPPNHRRSSTYALVLLNQRLPKFTPLLWKH-AQLRLCA 60

Query: 61  DGGANRVFDELPLLLPHLDALDVRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESED 120
           DGGANRVFD+LPL+ PHLDALDVRNSYKPDVIRGDMDSIRTEVL+FYAKQGTKIFDESED
Sbjct: 61  DGGANRVFDDLPLMFPHLDALDVRNSYKPDVIRGDMDSIRTEVLEFYAKQGTKIFDESED 120

Query: 121 QDTTDLHKCVAYILQSMPNEEGSNLCILVAGALGGRFDHEIGNINVLCRFSTTHIILLSD 180
           QDTTDLHKCVAYILQS+PN+E SNLCILVAGALGGRFDHEIGNINVLCRFSTT IILLSD
Sbjct: 121 QDTTDLHKCVAYILQSIPNQE-SNLCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSD 180

Query: 181 DCLIHLLPSTHHHEIHVHSSVEGPHCGLVPIGMPSGSTTTTGLQWDLTDTEMKFGGLIST 240
           DCLIHLLP THHHEI +HSSVEGPHCGL+PIGMPSGSTTTTGLQWDL+DTEMKFGGLIST
Sbjct: 181 DCLIHLLPRTHHHEILIHSSVEGPHCGLIPIGMPSGSTTTTGLQWDLSDTEMKFGGLIST 240

Query: 241 SNIVKGEKVTVQSDSDLLWTISLKS 266
           SNIVK EKVTVQSDSDLLWTISLKS
Sbjct: 241 SNIVKEEKVTVQSDSDLLWTISLKS 258

[Close]

BLAST of Spg013841 vs. ExPASy TrEMBL
Match: A0A6J1H6C2 (Thiamine pyrophosphokinase OS=Cucurbita moschata OX=3662 GN=LOC111460928 PE=3 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 5.8e-131
Identity = 237/271 (87.45%), Postives = 247/271 (91.14%), Query Frame = 0


Query: 1   MGVMFHSSDFLLPPPPPPPPSSSPADSRRSSTYALVLLNQRLPKFTPLLWKHAAQLRICA 60
           MGV FHSSDFLL     P  +SSPA    SSTYALVLLNQ LPKFTPLLWKH A+LR+CA
Sbjct: 1   MGVFFHSSDFLL-----PSSTSSPAGHPPSSTYALVLLNQSLPKFTPLLWKH-AKLRLCA 60

Query: 61  DGGANRVFDELPLLLPHLDALDVRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESED 120
           DGGANRVFDELPLL PH DA D+RNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESED
Sbjct: 61  DGGANRVFDELPLLFPHQDATDIRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESED 120

Query: 121 QDTTDLHKCVAYILQSMPNEEGSNLCILVAGALGGRFDHEIGNINVLCRFSTTHIILLSD 180
           QDTTDLHKCVAYILQSMPN+E SNLCILVAGALGGRFDHE+GNINVLCRFS+T IILLSD
Sbjct: 121 QDTTDLHKCVAYILQSMPNQE-SNLCILVAGALGGRFDHEMGNINVLCRFSSTRIILLSD 180

Query: 181 DCLIHLLPSTHHHEIHVHSSVEGPHCGLVPIGMPSGSTTTTGLQWDLTDTEMKFGGLIST 240
           DCLIHLLP THHHEIHVHSSVEGPHCGL+PIGMPSG TTTTGLQWDLTDTEMKFGGL+ST
Sbjct: 181 DCLIHLLPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDTEMKFGGLVST 240

Query: 241 SNIVKGEKVTVQSDSDLLWTISLKSNEITHS 272
           SNIVKGEKV+VQSDSDLLWTISLK NE  HS
Sbjct: 241 SNIVKGEKVSVQSDSDLLWTISLKGNETRHS 264

[Close]

BLAST of Spg013841 vs. ExPASy TrEMBL
Match: A0A6J1D8K1 (Thiamine pyrophosphokinase OS=Momordica charantia OX=3673 GN=LOC111017915 PE=3 SV=1)

HSP 1 Score: 474.6 bits (1220), Expect = 2.9e-130
Identity = 236/271 (87.08%), Postives = 248/271 (91.51%), Query Frame = 0


Query: 1   MGVMFHSSDFLLPPPPPPPPSSSPADSRRSSTYALVLLNQRLPKFTPLLWKHAAQLRICA 60
           M  MFHSSDFLL       PSS+PAD+RRSSTYALVLLNQRLP+FTPLLWKH AQLR+CA
Sbjct: 1   MAAMFHSSDFLL-------PSSTPADNRRSSTYALVLLNQRLPRFTPLLWKH-AQLRLCA 60

Query: 61  DGGANRVFDELPLLLPHLDALDVRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESED 120
           DGGANRV+DELP   PHLDALDVRNSYKPDVIRGDMDSIRTEVLDFYAK+GTK+FDESED
Sbjct: 61  DGGANRVYDELPSFFPHLDALDVRNSYKPDVIRGDMDSIRTEVLDFYAKKGTKVFDESED 120

Query: 121 QDTTDLHKCVAYILQSMPNEEGSNLCILVAGALGGRFDHEIGNINVLCRFSTTHIILLSD 180
           QDTTDLHKCVAYILQSMP++E SNLCI+VAGALGGRFDHEIGNINVLCRFSTT IILLSD
Sbjct: 121 QDTTDLHKCVAYILQSMPSQE-SNLCIVVAGALGGRFDHEIGNINVLCRFSTTRIILLSD 180

Query: 181 DCLIHLLPSTHHHEIHVHSSVEGPHCGLVPIGMPSGSTTTTGLQWDLTDTEMKFGGLIST 240
           DCLIHLLP T  HEI VHSSVEGPHCGL+PIGMPSGSTTTTGL+WDLTDTEMKFGGLIST
Sbjct: 181 DCLIHLLPKTRAHEIQVHSSVEGPHCGLIPIGMPSGSTTTTGLKWDLTDTEMKFGGLIST 240

Query: 241 SNIVKGEKVTVQSDSDLLWTISLKSNEITHS 272
           SNIVKGEKVTVQSDSDLLWTISLKS    HS
Sbjct: 241 SNIVKGEKVTVQSDSDLLWTISLKSTGTAHS 262

[Close]

BLAST of Spg013841 vs. ExPASy TrEMBL
Match: A0A0A0KAV4 (Thiamine pyrophosphokinase OS=Cucumis sativus OX=3659 GN=Csa_7G396410 PE=3 SV=1)

HSP 1 Score: 471.1 bits (1211), Expect = 3.2e-129
Identity = 237/266 (89.10%), Postives = 247/266 (92.86%), Query Frame = 0


Query: 1   MGVMFHSSDFLLPPPPPPPPSSSPADSRRSSTYALVLLNQRLPKFTPLLWKHAAQLRICA 60
           M V+FHSSDFLLP P     SS P D RRSSTYALVLLNQRLPKFTPLLWKH AQLR+CA
Sbjct: 1   MEVLFHSSDFLLPSPSSSSSSSLP-DRRRSSTYALVLLNQRLPKFTPLLWKH-AQLRLCA 60

Query: 61  DGGANRVFDELPLLLPHLDALDVRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESED 120
           DGGANRVFDE PL+ PHLDALDVRNSYKPDVIRGDMDSIRTEVL+FYA QGTKIFDESED
Sbjct: 61  DGGANRVFDEFPLMFPHLDALDVRNSYKPDVIRGDMDSIRTEVLEFYAMQGTKIFDESED 120

Query: 121 QDTTDLHKCVAYILQSMPNEEGSNLCILVAGALGGRFDHEIGNINVLCRFSTTHIILLSD 180
           QDTTDLHKCVAYILQS+PN+E SNLCILVAGALGGRFDHEIGNINVLCRFSTT IILLSD
Sbjct: 121 QDTTDLHKCVAYILQSIPNQE-SNLCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSD 180

Query: 181 DCLIHLLPSTHHHEIHVHSSVEGPHCGLVPIGMPSGSTTTTGLQWDLTDTEMKFGGLIST 240
           DCLIHLLP THHHEI VHSSVEGPHCGL+PIGMPSGSTTTTGL+WDL+DTEMKFGGLIST
Sbjct: 181 DCLIHLLPRTHHHEILVHSSVEGPHCGLIPIGMPSGSTTTTGLEWDLSDTEMKFGGLIST 240

Query: 241 SNIVKGEKVTVQSDSDLLWTISLKSN 267
           SNIVK EKVTVQSDSDLLWTISLKS+
Sbjct: 241 SNIVKEEKVTVQSDSDLLWTISLKSH 263

[Close]

BLAST of Spg013841 vs. TAIR 10
Match: AT2G44750.2 (thiamin pyrophosphokinase 2 )

HSP 1 Score: 366.3 bits (939), Expect = 2.1e-101
Identity = 175/264 (66.29%), Postives = 207/264 (78.41%), Query Frame = 0


Query: 1   MGVMFHSSDFLLPPPPPPPPSSSPADSRRSSTYALVLLNQRLPKFTPLLWKHAAQLRICA 60
           M VM HSS FLL           P D    + YALV+LNQ LP+FTPLLW+H A+LR+CA
Sbjct: 5   MDVMIHSSSFLL-----------PCDETCGTRYALVVLNQNLPRFTPLLWEH-AKLRLCA 64

Query: 61  DGGANRVFDELPLLLPHLDALDVRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESED 120
           DGGANR++DELPL  PH D   +RN YKPDVI+GDMDSIR +VLDFY   GTK+ DES D
Sbjct: 65  DGGANRIYDELPLFFPHEDPFVIRNRYKPDVIKGDMDSIRRDVLDFYVYWGTKVIDESHD 124

Query: 121 QDTTDLHKCVAYILQSMPNEEGSNLCILVAGALGGRFDHEIGNINVLCRFSTTHIILLSD 180
           QDTTDL KC++YI  S  N+E S L IL  GALGGRFDHE GN+NVL R+  T I+LLSD
Sbjct: 125 QDTTDLDKCISYIRHSTLNQESSRLQILATGALGGRFDHEAGNLNVLYRYPDTRIVLLSD 184

Query: 181 DCLIHLLPSTHHHEIHVHSSVEGPHCGLVPIGMPSGSTTTTGLQWDLTDTEMKFGGLIST 240
           DCLI LLP TH HEIH+HSS++GPHCGL+PIG PS +TTT+GL+WDL++TEM+FGGLIST
Sbjct: 185 DCLIQLLPKTHRHEIHIHSSLQGPHCGLIPIGTPSANTTTSGLKWDLSNTEMRFGGLIST 244

Query: 241 SNIVKGEKVTVQSDSDLLWTISLK 265
           SN+VK E +TV+SDSDLLWTIS+K
Sbjct: 245 SNLVKEEIITVESDSDLLWTISIK 256

[Close]

BLAST of Spg013841 vs. TAIR 10
Match: AT1G02880.2 (thiamin pyrophosphokinase1 )

HSP 1 Score: 365.2 bits (936), Expect = 4.7e-101
Identity = 177/269 (65.80%), Postives = 210/269 (78.07%), Query Frame = 0


Query: 1   MGVMFHSSDFLLPPPPPPPPSSSPADSRRSSTYALVLLNQRLPKFTPLLWKHAAQLRICA 60
           M VM HSS FLLP            ++   + YALV+LNQ LP+FTPLLW+H A+LR+CA
Sbjct: 1   MDVMIHSSSFLLPCD----------ETSTGTRYALVVLNQSLPRFTPLLWEH-AKLRLCA 60

Query: 61  DGGANRVFDELPLLLPHLDALDVRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESED 120
           DGGANR++DELPL  P+ DAL +RN YKPDVI+GDMDSIR +VLDFY   GTK+ DES D
Sbjct: 61  DGGANRIYDELPLFFPNEDALAIRNRYKPDVIKGDMDSIRRDVLDFYINLGTKVIDESHD 120

Query: 121 QDTTDLHKCVAYILQSMPNEEGSNLCILVAGALGGRFDHEIGNINVLCRFSTTHIILLSD 180
           QDTTDL KC+ YI  S  N+E S L IL  GALGGRFDHE GN+NVL R+  T I+LLSD
Sbjct: 121 QDTTDLDKCILYIRHSTLNQETSGLQILATGALGGRFDHEAGNLNVLYRYPDTRIVLLSD 180

Query: 181 DCLIHLLPSTHHHEIHVHSSVEGPHCGLVPIGMPSGSTTTTGLQWDLTDTEMKFGGLIST 240
           DCLI LLP TH HEIH+ SS+EGPHCGL+PIG PS  TTT+GLQWDL++TEM+FGGLIST
Sbjct: 181 DCLIQLLPKTHRHEIHIQSSLEGPHCGLIPIGTPSAKTTTSGLQWDLSNTEMRFGGLIST 240

Query: 241 SNIVKGEKVTVQSDSDLLWTISLKSNEIT 270
           SN+VK EK+TV+SDSDLLWTIS+K   ++
Sbjct: 241 SNLVKEEKITVESDSDLLWTISIKKTGLS 258

[Close]

BLAST of Spg013841 vs. TAIR 10
Match: AT1G02880.3 (thiamin pyrophosphokinase1 )

HSP 1 Score: 365.2 bits (936), Expect = 4.7e-101
Identity = 177/269 (65.80%), Postives = 210/269 (78.07%), Query Frame = 0


Query: 1   MGVMFHSSDFLLPPPPPPPPSSSPADSRRSSTYALVLLNQRLPKFTPLLWKHAAQLRICA 60
           M VM HSS FLLP            ++   + YALV+LNQ LP+FTPLLW+H A+LR+CA
Sbjct: 4   MDVMIHSSSFLLPCD----------ETSTGTRYALVVLNQSLPRFTPLLWEH-AKLRLCA 63

Query: 61  DGGANRVFDELPLLLPHLDALDVRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESED 120
           DGGANR++DELPL  P+ DAL +RN YKPDVI+GDMDSIR +VLDFY   GTK+ DES D
Sbjct: 64  DGGANRIYDELPLFFPNEDALAIRNRYKPDVIKGDMDSIRRDVLDFYINLGTKVIDESHD 123

Query: 121 QDTTDLHKCVAYILQSMPNEEGSNLCILVAGALGGRFDHEIGNINVLCRFSTTHIILLSD 180
           QDTTDL KC+ YI  S  N+E S L IL  GALGGRFDHE GN+NVL R+  T I+LLSD
Sbjct: 124 QDTTDLDKCILYIRHSTLNQETSGLQILATGALGGRFDHEAGNLNVLYRYPDTRIVLLSD 183

Query: 181 DCLIHLLPSTHHHEIHVHSSVEGPHCGLVPIGMPSGSTTTTGLQWDLTDTEMKFGGLIST 240
           DCLI LLP TH HEIH+ SS+EGPHCGL+PIG PS  TTT+GLQWDL++TEM+FGGLIST
Sbjct: 184 DCLIQLLPKTHRHEIHIQSSLEGPHCGLIPIGTPSAKTTTSGLQWDLSNTEMRFGGLIST 243

Query: 241 SNIVKGEKVTVQSDSDLLWTISLKSNEIT 270
           SN+VK EK+TV+SDSDLLWTIS+K   ++
Sbjct: 244 SNLVKEEKITVESDSDLLWTISIKKTGLS 261

[Close]

BLAST of Spg013841 vs. TAIR 10
Match: AT2G44750.1 (thiamin pyrophosphokinase 2 )

HSP 1 Score: 360.9 bits (925), Expect = 8.9e-100
Identity = 174/264 (65.91%), Postives = 206/264 (78.03%), Query Frame = 0


Query: 1   MGVMFHSSDFLLPPPPPPPPSSSPADSRRSSTYALVLLNQRLPKFTPLLWKHAAQLRICA 60
           M VM HSS FLL           P D    + YALV+LNQ LP+FTPLLW+H A+LR+CA
Sbjct: 5   MDVMIHSSSFLL-----------PCDETCGTRYALVVLNQNLPRFTPLLWEH-AKLRLCA 64

Query: 61  DGGANRVFDELPLLLPHLDALDVRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESED 120
           DGGANR++DELPL  PH D   +RN YKPDVI+GDMDSIR +VLDFY   GTK+ DES D
Sbjct: 65  DGGANRIYDELPLFFPHEDPFVIRNRYKPDVIKGDMDSIRRDVLDFYVYWGTKVIDESHD 124

Query: 121 QDTTDLHKCVAYILQSMPNEEGSNLCILVAGALGGRFDHEIGNINVLCRFSTTHIILLSD 180
           QDTTDL KC++YI  S  N+E S   IL  GALGGRFDHE GN+NVL R+  T I+LLSD
Sbjct: 125 QDTTDLDKCISYIRHSTLNQESSR--ILATGALGGRFDHEAGNLNVLYRYPDTRIVLLSD 184

Query: 181 DCLIHLLPSTHHHEIHVHSSVEGPHCGLVPIGMPSGSTTTTGLQWDLTDTEMKFGGLIST 240
           DCLI LLP TH HEIH+HSS++GPHCGL+PIG PS +TTT+GL+WDL++TEM+FGGLIST
Sbjct: 185 DCLIQLLPKTHRHEIHIHSSLQGPHCGLIPIGTPSANTTTSGLKWDLSNTEMRFGGLIST 244

Query: 241 SNIVKGEKVTVQSDSDLLWTISLK 265
           SN+VK E +TV+SDSDLLWTIS+K
Sbjct: 245 SNLVKEEIITVESDSDLLWTISIK 254

[Close]

BLAST of Spg013841 vs. TAIR 10
Match: AT1G02880.1 (thiamin pyrophosphokinase1 )

HSP 1 Score: 273.5 bits (698), Expect = 1.9e-73
Identity = 129/183 (70.49%), Postives = 150/183 (81.97%), Query Frame = 0


Query: 87  YKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESEDQDTTDLHKCVAYILQSMPNEEGSNLC 146
           YKPDVI+GDMDSIR +VLDFY   GTK+ DES DQDTTDL KC+ YI  S  N+E S L 
Sbjct: 9   YKPDVIKGDMDSIRRDVLDFYINLGTKVIDESHDQDTTDLDKCILYIRHSTLNQETSGLQ 68

Query: 147 ILVAGALGGRFDHEIGNINVLCRFSTTHIILLSDDCLIHLLPSTHHHEIHVHSSVEGPHC 206
           IL  GALGGRFDHE GN+NVL R+  T I+LLSDDCLI LLP TH HEIH+ SS+EGPHC
Sbjct: 69  ILATGALGGRFDHEAGNLNVLYRYPDTRIVLLSDDCLIQLLPKTHRHEIHIQSSLEGPHC 128

Query: 207 GLVPIGMPSGSTTTTGLQWDLTDTEMKFGGLISTSNIVKGEKVTVQSDSDLLWTISLKSN 266
           GL+PIG PS  TTT+GLQWDL++TEM+FGGLISTSN+VK EK+TV+SDSDLLWTIS+K  
Sbjct: 129 GLIPIGTPSAKTTTSGLQWDLSNTEMRFGGLISTSNLVKEEKITVESDSDLLWTISIKKT 188

Query: 267 EIT 270
            ++
Sbjct: 189 GLS 191

The following BLAST results are available for this feature:

BLAST of Spg013841 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7030224.1	1.7e-132	87.82	Thiamine pyrophosphokinase 1 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023515266.1	3.7e-132	87.45	thiamine pyrophosphokinase 1 isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_023004625.1	1.9e-131	88.39	thiamine pyrophosphokinase 1 isoform X1 [Cucurbita maxima]	[more]
KAG6593191.1	4.1e-131	87.50	Thiamine pyrophosphokinase 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_008451592.1	7.0e-131	89.81	PREDICTED: thiamine pyrophosphokinase 1 isoform X1 [Cucumis melo]	[more]

BLAST of Spg013841 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4IV16	3.0e-100	66.29	Thiamine pyrophosphokinase 2 OS=Arabidopsis thaliana OX=3702 GN=TPK2 PE=2 SV=1	[more]
B9DGU7	6.7e-100	65.80	Thiamine pyrophosphokinase 1 OS=Arabidopsis thaliana OX=3702 GN=TPK1 PE=2 SV=1	[more]
Q5JK24	9.7e-91	65.02	Thiamine pyrophosphokinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=TPK1 PE...	[more]
Q60DX1	1.5e-88	62.74	Thiamine pyrophosphokinase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=TPK3 PE...	[more]
Q0JMW0	2.0e-88	61.89	Thiamine pyrophosphokinase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=TPK2 PE...	[more]

BLAST of Spg013841 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KV43	9.0e-132	88.39	Thiamine pyrophosphokinase OS=Cucurbita maxima OX=3661 GN=LOC111497866 PE=3 SV=1	[more]
A0A1S3BSZ4	3.4e-131	89.81	Thiamine pyrophosphokinase OS=Cucumis melo OX=3656 GN=LOC103492826 PE=3 SV=1	[more]
A0A6J1H6C2	5.8e-131	87.45	Thiamine pyrophosphokinase OS=Cucurbita moschata OX=3662 GN=LOC111460928 PE=3 SV...	[more]
A0A6J1D8K1	2.9e-130	87.08	Thiamine pyrophosphokinase OS=Momordica charantia OX=3673 GN=LOC111017915 PE=3 S...	[more]
A0A0A0KAV4	3.2e-129	89.10	Thiamine pyrophosphokinase OS=Cucumis sativus OX=3659 GN=Csa_7G396410 PE=3 SV=1	[more]

BLAST of Spg013841 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G44750.2	2.1e-101	66.29	thiamin pyrophosphokinase 2	[more]
AT1G02880.2	4.7e-101	65.80	thiamin pyrophosphokinase1	[more]
AT1G02880.3	4.7e-101	65.80	thiamin pyrophosphokinase1	[more]
AT2G44750.1	8.9e-100	65.91	thiamin pyrophosphokinase 2	[more]
AT1G02880.1	1.9e-73	70.49	thiamin pyrophosphokinase1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007373	Thiamin pyrophosphokinase, thiamin-binding domain	SMART	SM00983	TPK_B1_binding_a_2_a	coord: 191..257 e-value: 1.7E-11 score: 54.2
IPR007373	Thiamin pyrophosphokinase, thiamin-binding domain	PFAM	PF04265	TPK_B1_binding	coord: 192..258 e-value: 2.6E-22 score: 78.3
IPR006282	Thiamin pyrophosphokinase	TIGRFAM	TIGR01378	TIGR01378	coord: 35..261 e-value: 2.9E-60 score: 201.2
IPR007371	Thiamin pyrophosphokinase, catalytic domain	PFAM	PF04263	TPK_catalytic	coord: 48..174 e-value: 3.5E-39 score: 133.6
IPR007371	Thiamin pyrophosphokinase, catalytic domain	CDD	cd07995	TPK	coord: 34..261 e-value: 1.83232E-58 score: 183.515
None	No IPR available	GENE3D	2.60.120.320		coord: 175..265 e-value: 5.3E-30 score: 105.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 10..24
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..28
None	No IPR available	PANTHER	PTHR13622	THIAMIN PYROPHOSPHOKINASE	coord: 3..264
None	No IPR available	PANTHER	PTHR13622:SF12	THIAMINE PYROPHOSPHOKINASE 1	coord: 3..264
IPR036759	Thiamin pyrophosphokinase, catalytic domain superfamily	GENE3D	3.40.50.10240	Thiamin pyrophosphokinase, catalytic domain	coord: 31..174 e-value: 5.0E-44 score: 152.1
IPR036759	Thiamin pyrophosphokinase, catalytic domain superfamily	SUPERFAMILY	63999	Thiamin pyrophosphokinase, catalytic domain	coord: 25..183
IPR016966	Thiamin pyrophosphokinase, eukaryotic	PIRSF	PIRSF031057	TPK1	coord: 17..271 e-value: 2.2E-66 score: 221.7
IPR036371	Thiamin pyrophosphokinase, thiamin-binding domain superfamily	SUPERFAMILY	63862	Thiamin pyrophosphokinase, substrate-binding domain	coord: 183..267

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg013841.1	Spg013841.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006298	mismatch repair
biological_process	GO:0000398	mRNA splicing, via spliceosome
biological_process	GO:0000956	nuclear-transcribed mRNA catabolic process
biological_process	GO:0016310	phosphorylation
biological_process	GO:0009229	thiamine diphosphate biosynthetic process
biological_process	GO:0006772	thiamine metabolic process
cellular_component	GO:0005829	cytosol
cellular_component	GO:0032300	mismatch repair complex
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016887	ATP hydrolysis activity
molecular_function	GO:0016301	kinase activity
molecular_function	GO:0030983	mismatched DNA binding
molecular_function	GO:0030975	thiamine binding
molecular_function	GO:0004788	thiamine diphosphokinase activity