Spg013836 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg013836
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Cytochrome p450
Location	scaffold3: 48938655 .. 48942637 (-)
RNA-Seq Expression	Spg013836
Synteny	Spg013836

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGGTCAGAGCTGTGGTGATGATGATTAAGCATATTTAAGAGAAGTACAGAGAAAGATAGAGAGACAGACAGGCAGGGGAGGCAGAGGAAATCTTTGTCCAAAATGGACAGTTTATGGGATGTTGTGGTTGTGACAGCCATTCTTTTGACCACCATTATTCTCTATAGGAACTGCTTTAGACTTCTCAGATCAAAATTCTGCAACCAACTCCCTCTAGGCTCTCTTGGCTGGCCTTTCATTGGTGAGACTATTGAGTTCATCTCCTGTGCTTATTCCGACCGCCCTGAAACCTTCATGGACAAGCGTCGTCGACTGTAAGTTATCCACAAAAACGAACTTCGTGACATCCCAATTTCTTCTAATGCTGATATGACTTGTGGTTGTTTGTCAATGGAGTTGCTTAGTTTTCTTATGAACCGGTGCTTCATTTTGGTTTGCAGGTATGGGAAGGTGTTCAAGTCACATATTTTTGGAAGTCCTACCATTGTTTCTACAGATGCAGAAGTCAGTAAGTTCATTCTGCAGAGTGATGCAAAGGCTTTCGTACCATCTTACCCGAAATCTTTGACAGAATTGATGGGGAAATCTTCCATTTTGCTGATAAACGGGAGCTTGCAGAAGAGAATCCATGGTCTTGTGGGAGCTTTCTTCAAGTCTTCACATCTCAAAGCTCAAATAACCAAGGATATGGAGTGTTATGTCAAACTATCAATGGCAAATTGGACTGAGAACAATCCTATTTACCTTCAAGATGAAACCAAAAATGTCAGCTTTCTCTCTCTTTCTCTCCCTCTTTTGAAACTTGAAATTAACCTGTGAATTCTCTTTTTTCCCCCTTACATGAAGTTAACAAAAAGACAATAGATAAAAAATGTCCCACCATTACTGTGCTGATACTGATACTAGACAAAAACAGTGACTTTCTTTACCGGATTTGAGTGAATCATTGATGTCCAAATTTCTCTAGCTCCTACTTGGGTTTAAAGACGATTTCTGTCAAAAGTAAGAGAACCCCACAGTTTTTTTTTTTGCTATCATTCTGTTCAAATCATAGTGTCCAAGAATTTACCTTTGTAATCATTAGCTATCTATTTTCCTGTTCAAAAAGTTACTAAGTGAACCCCTTTTGTCTCCAGATTTCCTTTCAAGTATTAGTAAAGATATTGATTAGTTTAAATCCTGGTCAAAGGATGGAGTTTCTCAAGCAACAGTTTCAAGAATTCATCGCGGGGCTTATGGCTTTGCCAGTGAACATTCCCGGAAGCAGGCTTTACCGATCGTTGCAGGCAAGTTGGTCGTAAAAGGTTTAATATCCTCCTTTACTAGTAACTTTTTACTATACTCTAAAAAATGATGGAGCTTCATTTTATTCTTGAATTTCAGGCCAAAAGGAAAATGGTTAAACTAGTAAAGCAGATAATACAAGAAAGAAAAAGCAGTGGAATTTCCCCTGTCCCAAGAGATGTCATGGAAGTTTTGCTCAAAGATGAAAGTGAACAACTAACAGATGATCTAATAGCAGTGAACATGATTGACATGATGATTCCTGGGGAAGATTCAGTGCCAATCTTAATGACTCTTGCAGTAAAATACCTCTCCGATTGCCCAACTGCACTTCATCAGTTAACGGTACGCTATTTTTCTTACCAACTCTCGATCTTTATTACTGTTATGTTAAATTACTACTGAACGCAAAAGCTTAAGTTGATGAGTTATGATAATTGCACTTTAATCACTTGTGGGCTTGGGGTCCCAATGAGTGATTATCAATGTTAATGACACTACAGGATTTCGAATATACAACCTTTTACTTTGATACCATGTTAGAATCACAATCAAAGCTTAAGCTGATGGGATACAACTTACAATAGACTTAATCTTTTAGACTGTCCTTCCAATGGAATACGAATATACTCATCGAATTTATCTAGTTGTACTTTCTATCAATTGGAAAGGAAATTTCCTTGTCCACTTTTACAATGTCCCTTTCTGACGTGGTCCTTTTATGCCTGTCACATGGTCCTCAGGTGCGTTAGCTAGATTGATGTCGAACCGACTTCCTAAATTACTAATTGTTCTGCAAGTTCATGTATGAATTAATTTTATGGAAACCATGGCTAGGATACAAGTTAAATGCATACATTCGTGTCTATTAGTAACACTTAGTGTAAAGCAAAGAAGGTTAATCTATGCTCTCTCTCGGTTTCTTTGAATAGTTCTTAAACGATATGCTTGTTTGGACCCGTATCAGGAGGAAAATTTGAAGATGAAAAGACTCAAAGAGCAGTTCGGAGAGCCGTTGCATTGGAGCGATTACTTATCACTGCCATTTACGCAGAGTGTAATACATTAACCTTTTGATAAAGTAGAAAGACCAGAATGCTTGCAGTATCATGATTGTATTGAATATGGTTTCATATGATTAGGTGATCACGGAGACACTAAGGATGGGAAACATCATAATCGGGGTGATGCGGAAGGCCATGAAAGACGTGAAGATAAAAGGATACCTAATACCGAAAGGTTGGTCGGTGCTTACGTACTTTAGATCCGTTCATCTCGACGACAATCACTATGACTGGCCTTACAAATTCAATCCATGGAGGTGGCAAGTAAGAAGGAACATATTTCTCTCTGTTTCTTTTTCTATCTTTTGGTTTTTATGTTTGTTTCTCCCTAATGGGTTTGTGGTCCAACAGGATAGAGACTTGAGTAGTTGTAACTTCACTCCTTTTGGAGGTGGACAAAGACTATGCCCTGGACTTGACTTGGCCAGGCTTGAAGCTTCCATCTTCCTTCATCACTTTGTCACTCAGTTCAGGTAAACTGCCAAAAATCACTTTTCCACCTCAACTTTTAGTTTTTACTGTTTTTCTCATTTCAGTTGGAATGAAAACAAAACCACTCAAAAAGTCCGGTGCCATATCTATCCCATTAGATATTTGATTTCGTGCCTTGTGTTCTTTTTTAAACAATACTTGACTGTATACCGATACGTAGAGGTCTCCCTACATATCTCATTAAAATCTTGACAACTAAGAAAGAGAAAGGTAAAAATGAGAACATTTTTTTATCTCTCACGGTTTTCAACATTTTAATGGTGGTATGTGGGAAGATTTCTACATATCGATATGCTCCCAAGTTTTTTCTTTCTTTTTTATAATTGGAGTGTTAGGACCTATTCCTTACTTTATGCTCATAGCCCACCCCACTAATCCCTATAAAGGCTTTGTCCATCCCAAGACTAGCTAGCTCTTGGGAGACAACAAAGATTTGATATCTTCTGTTAAATTTTCTTTCTAGTACTTTTTCCCTGCTTTTACAAAATTATCTTATATATATATATATATATTCTCTCCCAATCTCACACATATAACTTGTCTATATATATCTTGTTCGAATACGAGCGTAAAGATGGCTTTCGAATAATTTTCTAACTGAACCATATGGCATATTACAGATGGATTGCTGAGGAAGATAGAATCATAAACTTCCCCACAGTGAGGATGAAGAAGAGGATGCCAGTTTGGGTGAAGAGGAGAAGGGACTCATGACAAGGCCAAGGAGACCTGCCTTTTTGCCAAAACCACAACTTCAATTAACTTCACCAATTACACAACAACCCAACATACCTTTGAATAATAATCCCACATTTAACCAAGGGAATGTGGGTAAAATAGAGGCCAGTTCAAACATGACTCCGAAAGATCGATAAAATCCGAAAGGTCCCATTCAACATGGCTCGTAGTTTACATTAGGGTTGTTCGTCGGTCGGTCGAGTCAGTTTTCAGTCTATTTTTTAACACCCCTAATTTACTTACATGCTTGTTTGTTGTGTATTATAAATGATATATGTGTGTGTATAGTGTACCTATTTGAATTGATAGTCATAGGTCTTGACAAGTAATGTGTAATATAGTTACTGGTTATATTGTTTTGATCTTTCTAAATATTTTTTGTGATATGTAGATCATCGAGTT

mRNA sequence

ATGGACAGTTTATGGGATGTTGTGGTTGTGACAGCCATTCTTTTGACCACCATTATTCTCTATAGGAACTGCTTTAGACTTCTCAGATCAAAATTCTGCAACCAACTCCCTCTAGGCTCTCTTGGCTGGCCTTTCATTGGTGAGACTATTGAGTTCATCTCCTGTGCTTATTCCGACCGCCCTGAAACCTTCATGGACAAGCGTCGTCGACTGTATGGGAAGGTGTTCAAGTCACATATTTTTGGAAGTCCTACCATTGTTTCTACAGATGCAGAAGTCAGTAAGTTCATTCTGCAGAGTGATGCAAAGGCTTTCGTACCATCTTACCCGAAATCTTTGACAGAATTGATGGGGAAATCTTCCATTTTGCTGATAAACGGGAGCTTGCAGAAGAGAATCCATGGTCTTGTGGGAGCTTTCTTCAAGTCTTCACATCTCAAAGCTCAAATAACCAAGGATATGGAGTGTTATGATGGAGTTTCTCAAGCAACAGTTTCAAGAATTCATCGCGGGGCTTATGGCTTTGCCAGTGAACATTCCCGGAAGCAGGCTTTACCGATCGTTGCAGGCAAGTTGGCCAAAAGGAAAATGGTTAAACTAGTAAAGCAGATAATACAAGAAAGAAAAAGCAGTGGAATTTCCCCTGTCCCAAGAGATGTCATGGAAGTTTTGCTCAAAGATGAAAGTGAACAACTAACAGATGATCTAATAGCAGTGAACATGATTGACATGATGATTCCTGGGGAAGATTCAGTGCCAATCTTAATGACTCTTGCAGTAAAATACCTCTCCGATTGCCCAACTGCACTTCATCAGTTAACGGTGATCACGGAGACACTAAGGATGGGAAACATCATAATCGGGGTGATGCGGAAGGCCATGAAAGACGTGAAGATAAAAGGATACCTAATACCGAAAGGTTGGTCGGTGCTTACGTACTTTAGATCCGTTCATCTCGACGACAATCACTATGACTGGCCTTACAAATTCAATCCATGGAGGTGGCAAGATAGAGACTTGAGTAGTTGTAACTTCACTCCTTTTGGAGGTGGACAAAGACTATGCCCTGGACTTGACTTGGCCAGGCTTGAAGCTTCCATCTTCCTTCATCACTTTGTCACTCAGTTCAGATGGATTGCTGAGGAAGATAGAATCATAAACTTCCCCACAGTGAGGATGAAGAAGAGGATGCCAGTTTGGGTGAAGAGGAGAAGGGACTCATGA

Coding sequence (CDS)

ATGGACAGTTTATGGGATGTTGTGGTTGTGACAGCCATTCTTTTGACCACCATTATTCTCTATAGGAACTGCTTTAGACTTCTCAGATCAAAATTCTGCAACCAACTCCCTCTAGGCTCTCTTGGCTGGCCTTTCATTGGTGAGACTATTGAGTTCATCTCCTGTGCTTATTCCGACCGCCCTGAAACCTTCATGGACAAGCGTCGTCGACTGTATGGGAAGGTGTTCAAGTCACATATTTTTGGAAGTCCTACCATTGTTTCTACAGATGCAGAAGTCAGTAAGTTCATTCTGCAGAGTGATGCAAAGGCTTTCGTACCATCTTACCCGAAATCTTTGACAGAATTGATGGGGAAATCTTCCATTTTGCTGATAAACGGGAGCTTGCAGAAGAGAATCCATGGTCTTGTGGGAGCTTTCTTCAAGTCTTCACATCTCAAAGCTCAAATAACCAAGGATATGGAGTGTTATGATGGAGTTTCTCAAGCAACAGTTTCAAGAATTCATCGCGGGGCTTATGGCTTTGCCAGTGAACATTCCCGGAAGCAGGCTTTACCGATCGTTGCAGGCAAGTTGGCCAAAAGGAAAATGGTTAAACTAGTAAAGCAGATAATACAAGAAAGAAAAAGCAGTGGAATTTCCCCTGTCCCAAGAGATGTCATGGAAGTTTTGCTCAAAGATGAAAGTGAACAACTAACAGATGATCTAATAGCAGTGAACATGATTGACATGATGATTCCTGGGGAAGATTCAGTGCCAATCTTAATGACTCTTGCAGTAAAATACCTCTCCGATTGCCCAACTGCACTTCATCAGTTAACGGTGATCACGGAGACACTAAGGATGGGAAACATCATAATCGGGGTGATGCGGAAGGCCATGAAAGACGTGAAGATAAAAGGATACCTAATACCGAAAGGTTGGTCGGTGCTTACGTACTTTAGATCCGTTCATCTCGACGACAATCACTATGACTGGCCTTACAAATTCAATCCATGGAGGTGGCAAGATAGAGACTTGAGTAGTTGTAACTTCACTCCTTTTGGAGGTGGACAAAGACTATGCCCTGGACTTGACTTGGCCAGGCTTGAAGCTTCCATCTTCCTTCATCACTTTGTCACTCAGTTCAGATGGATTGCTGAGGAAGATAGAATCATAAACTTCCCCACAGTGAGGATGAAGAAGAGGATGCCAGTTTGGGTGAAGAGGAGAAGGGACTCATGA

Protein sequence

MDSLWDVVVVTAILLTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDRPETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKSSILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYDGVSQATVSRIHRGAYGFASEHSRKQALPIVAGKLAKRKMVKLVKQIIQERKSSGISPVPRDVMEVLLKDESEQLTDDLIAVNMIDMMIPGEDSVPILMTLAVKYLSDCPTALHQLTVITETLRMGNIIIGVMRKAMKDVKIKGYLIPKGWSVLTYFRSVHLDDNHYDWPYKFNPWRWQDRDLSSCNFTPFGGGQRLCPGLDLARLEASIFLHHFVTQFRWIAEEDRIINFPTVRMKKRMPVWVKRRRDS

Homology

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BLAST of Spg013836 vs. NCBI nr
Match: XP_008462361.1 (PREDICTED: 3-epi-6-deoxocathasterone 23-monooxygenase [Cucumis melo])

HSP 1 Score: 671.8 bits (1732), Expect = 3.8e-189
Identity = 359/473 (75.90%), Postives = 375/473 (79.28%), Query Frame = 0


Query: 1   MDSLWDVVVVTAILLTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60
           MDSLWDV+VVTAI   TIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR
Sbjct: 1   MDSLWDVLVVTAI---TIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60

Query: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120
           PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYDGVSQAT---------------- 180
           SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECY  +S A+                
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMASWTENNPIYLQDETKNI 180

Query: 181 -----VSRIHRGAYGFASEHSRKQALPIVAGKL----------------AKRKMVKLVKQ 240
                V  +     G + E  +KQ    +AG +                AKR MVKLVK+
Sbjct: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK 240

Query: 241 IIQERKSSGISPVPRDVMEVLLKDESEQLTDDLIAVNMIDMMIPGEDSVPILMTLAVKYL 300
           IIQERKS GIS VP+DVMEVLLKDE+E+LTDDLIA NMIDMMIPGEDSVPILMTLAVKYL
Sbjct: 241 IIQERKSCGISTVPKDVMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300

Query: 301 SDCPTALHQLT------------------------------VITETLRMGNIIIGVMRKA 360
           SDCP AL QLT                              VITETLRMGNIIIGVMRKA
Sbjct: 301 SDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRKA 360

Query: 361 MKDVKIKGYLIPKGWSVLTYFRSVHLDDNHYDWPYKFNPWRWQDRDLSSCNFTPFGGGQR 407
           MKD+KIKGYLI KGWSVLTYFRSVHLDDNHYD PYKFNPWRWQ+R+LSSCNFTPFGGGQR
Sbjct: 361 MKDMKIKGYLILKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQERELSSCNFTPFGGGQR 420

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BLAST of Spg013836 vs. NCBI nr
Match: KAG6575481.1 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 634.0 bits (1634), Expect = 8.7e-178
Identity = 343/504 (68.06%), Postives = 367/504 (72.82%), Query Frame = 0


Query: 1   MDSLWDVVVVTAILLTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60
           MDS WDV+V+TAI LT I+LYRNCF LLRSK C+ LPLGSLGWPFIGET+EFI+CAYSDR
Sbjct: 1   MDSFWDVLVLTAIFLTIILLYRNCFSLLRSKLCSHLPLGSLGWPFIGETLEFIACAYSDR 60

Query: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120
           PE FMDKRRRLYGKVFKSH+FGSPTIVSTDAEVSKFILQSDAKAFVP YPKSLTELMGKS
Sbjct: 61  PEAFMDKRRRLYGKVFKSHVFGSPTIVSTDAEVSKFILQSDAKAFVPCYPKSLTELMGKS 120

Query: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYDGVSQATVSRIHRGAY------- 180
           SILLINGSLQKRIHGLVGAFFKSS+LKAQITKDMECY  ++ A  S  +   Y       
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSYLKAQITKDMECYVKLAMANWSE-NSPVYLQDETKN 180

Query: 181 ---------------GFASEHSRKQALPIVAGKL-------------------------- 240
                          G   E  ++Q    +AG +                          
Sbjct: 181 ISFQVLVKILISLNPGQRMEFVKQQFQEFIAGLMALPVNIPGSRLYQSLQASWIVKALPS 240

Query: 241 ------------------AKRKMVKLVKQIIQERKSSGISP--VPRDVMEVLLKDESEQL 300
                             AKR MVKLVK+IIQER+S G SP  VP+DVMEVLLKDESE L
Sbjct: 241 SFTITIFLMELPFMLELQAKRAMVKLVKKIIQERRSCGSSPVAVPQDVMEVLLKDESELL 300

Query: 301 TDDLIAVNMIDMMIPGEDSVPILMTLAVKYLSDCPTALHQLT------------------ 360
           TDDLIA NMIDMMIPGEDSVPILMTLAVKYLSDCP AL QLT                  
Sbjct: 301 TDDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCPEALQQLTEENLKMKRLKEQAGEALH 360

Query: 361 ------------VITETLRMGNIIIGVMRKAMKDVKIKGYLIPKGWSVLTYFRSVHLDDN 407
                       VITETLRMGNIIIGVMRKAMKD+ IKGYLIPKGWSVLTYFRSVHLDD+
Sbjct: 361 WSDYLSMPFTQNVITETLRMGNIIIGVMRKAMKDMNIKGYLIPKGWSVLTYFRSVHLDDS 420

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BLAST of Spg013836 vs. NCBI nr
Match: XP_038898401.1 (LOW QUALITY PROTEIN: cytochrome P450 90D2-like [Benincasa hispida])

HSP 1 Score: 627.9 bits (1618), Expect = 6.3e-176
Identity = 338/460 (73.48%), Postives = 355/460 (77.17%), Query Frame = 0


Query: 1   MDSLWDVVVVTAILLTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60
           MDSLWDV+VVTAI  TTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR
Sbjct: 1   MDSLWDVLVVTAIFFTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60

Query: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120
           PETFM+KRRRLYGKVFKSHIFGSPTIVSTD+EVSKFILQSDAKAFVPSYPKSLTELMGKS
Sbjct: 61  PETFMNKRRRLYGKVFKSHIFGSPTIVSTDSEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYDGVSQAT---------------- 180
           SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECY  +S A                 
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVQLSMANWTENNLIYLQDETKNI 180

Query: 181 -----VSRIHRGAYGFASEHSRKQALPIVAGKL----------------AKRKMVKLVKQ 240
                V  +     G   E  +KQ    +AG +                AKR MVKLVK+
Sbjct: 181 SFQILVKILISLNPGQRMEFLKKQFQDFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK 240

Query: 241 IIQERKSSGISPVPRDVMEVLLKDESEQLTDDLIAVNMIDMMIPGEDSVPILMTLAVKYL 300
           IIQERKS GISPVP+DVMEVLLKDE+E+LTDDLIA NMIDMMIPGEDSVPILMTLAVKYL
Sbjct: 241 IIQERKSCGISPVPKDVMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300

Query: 301 SDCPTALHQLT------------------------------VITETLRMGNIIIGVMRKA 360
           SDCP AL QLT                              VITETLRMGNIIIGVMRKA
Sbjct: 301 SDCPAALQQLTEENLKMKGLKEQLGETLHWSDYLSMPFTQSVITETLRMGNIIIGVMRKA 360

Query: 361 MKDVKIKGYLIPKGWSVLTYFRSVHLDDNHYDWPYKFNPWRWQDRDLSSCNFTPFGGGQR 394
           MKD+KIKGYLIPKGWSVLTYFRSVHLDDNHYD PYKFNPWRWQDRDLSSCNFTPFGGGQR
Sbjct: 361 MKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQDRDLSSCNFTPFGGGQR 420

[Close]

BLAST of Spg013836 vs. NCBI nr
Match: XP_011659645.1 (LOW QUALITY PROTEIN: 3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 [Cucumis sativus])

HSP 1 Score: 621.7 bits (1602), Expect = 4.5e-174
Identity = 336/466 (72.10%), Postives = 355/466 (76.18%), Query Frame = 0


Query: 1   MDSLWDVVVVTAILLTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60
           MDSLWDV+VVTAI  TTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFIS AYSDR
Sbjct: 1   MDSLWDVLVVTAIFFTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISSAYSDR 60

Query: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120
           PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYDGVS------------------- 180
           SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECY  +S                   
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMANWTENNPIYLQDETKNI 180

Query: 181 --QATVSRIHRGAYGFASEHSRKQALPIVAGKL----------------AKRKMVKLVKQ 240
             Q  V  +     G + E  +KQ    +AG +                AKR MVKLVK+
Sbjct: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK 240

Query: 241 IIQERKSSGISPVPRDVMEVLLKDESEQLTDDLIAVNMIDMMIPGEDSVPILMTLAVKYL 300
           IIQERKS GIS VP+D+MEVLLKDE+E+LTDDLIA NMIDMMIPGEDSVPILMTLAVKYL
Sbjct: 241 IIQERKSCGISTVPKDIMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300

Query: 301 SDCPTALHQLT------------------------------VITETLRMGNIIIGVMRKA 360
           SDCP AL QLT                              VITETLRMGNIIIGVMRKA
Sbjct: 301 SDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRKA 360

Query: 361 MKDVKIKGYLIPKGWSVLTYFRSVHLDDNHYDWPYKFNPWRWQDRDLSSCNFTPFGGGQR 400
           MKD+KIKGYLIPKGWSVLTYFRSVHLDDNHYD PYKFNPWRWQDR++SSCNFTPFGGGQR
Sbjct: 361 MKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQDREMSSCNFTPFGGGQR 420

[Close]

BLAST of Spg013836 vs. NCBI nr
Match: KAE8646515.1 (hypothetical protein Csa_016429 [Cucumis sativus])

HSP 1 Score: 619.4 bits (1596), Expect = 2.2e-173
Identity = 331/444 (74.55%), Postives = 346/444 (77.93%), Query Frame = 0


Query: 1   MDSLWDVVVVTAILLTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60
           MDSLWDV+VVTAI  TTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFIS AYSDR
Sbjct: 1   MDSLWDVLVVTAIFFTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISSAYSDR 60

Query: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120
           PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYDGVS------------------- 180
           SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECY  +S                   
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMANWTENNPIYLQDETKNI 180

Query: 181 --QATVSRIHRGAYGFASEHSRKQALPIVAGKL----------------AKRKMVKLVKQ 240
             Q  V  +     G + E  +KQ    +AG +                AKR MVKLVK+
Sbjct: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK 240

Query: 241 IIQERKSSGISPVPRDVMEVLLKDESEQLTDDLIAVNMIDMMIPGEDSVPILMTLAVKYL 300
           IIQERKS GIS VP+D+MEVLLKDE+E+LTDDLIA NMIDMMIPGEDSVPILMTLAVKYL
Sbjct: 241 IIQERKSCGISTVPKDIMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300

Query: 301 SDCPTALHQLT------------------------------VITETLRMGNIIIGVMRKA 360
           SDCP AL QLT                              VITETLRMGNIIIGVMRKA
Sbjct: 301 SDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRKA 360

Query: 361 MKDVKIKGYLIPKGWSVLTYFRSVHLDDNHYDWPYKFNPWRWQDRDLSSCNFTPFGGGQR 378
           MKD+KIKGYLIPKGWSVLTYFRSVHLDDNHYD PYKFNPWRWQDR++SSCNFTPFGGGQR
Sbjct: 361 MKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQDREMSSCNFTPFGGGQR 420

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BLAST of Spg013836 vs. ExPASy Swiss-Prot
Match: Q94IW5 (Cytochrome P450 90D2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90D2 PE=1 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 5.4e-130
Identity = 245/442 (55.43%), Postives = 313/442 (70.81%), Query Frame = 0


Query: 35  QLPLGSLGWPFIGETIEFISCAYSDRPETFMDKRRRLYGK-VFKSHIFGSPTIVSTDAEV 94
           +LP GS GWP +GET+EF+SCAYS RPE F+DKRR+L+G  VF+SH+FGS T+V+ DAEV
Sbjct: 48  RLPPGSFGWPVVGETLEFVSCAYSPRPEAFVDKRRKLHGSAVFRSHLFGSATVVTADAEV 107

Query: 95  SKFILQSDAKAFVPSYPKSLTELMGKSSILLINGSLQKRIHGLVGAFFKSSHLKAQITKD 154
           S+F+LQSDA+AFVP YP+SLTELMGKSSILLING+LQ+R+HGLVGAFFKSSHLK+Q+T D
Sbjct: 108 SRFVLQSDARAFVPWYPRSLTELMGKSSILLINGALQRRVHGLVGAFFKSSHLKSQLTAD 167

Query: 155 ME----------------CYDGVSQATVSRI-HRGAYGFASEHSRKQ------------- 214
           M                     ++++ V  I  RG  G  +    +Q             
Sbjct: 168 MRRRLSPALSSFPDSSLLHVQHLAKSVVFEILVRGLIGLEAGEEMQQLKQQFQEFIVGLM 227

Query: 215 ALPI-------VAGKLAKRKMVKLVKQIIQERKS--SGISPVPRDVMEVLLKDESEQLTD 274
           +LPI            AK+KM +L+++II+E+++  +  SP PRD ++VL+ D S++LTD
Sbjct: 228 SLPIKLPGTRLYRSLQAKKKMARLIQRIIREKRARRAAASP-PRDAIDVLIGDGSDELTD 287

Query: 275 DLIAVNMIDMMIPGEDSVPILMTLAVKYLSDCPTALHQLT-------------------- 334
           +LI+ NMID+MIP EDSVP+L+TLAVK+LS+CP ALHQL                     
Sbjct: 288 ELISDNMIDLMIPAEDSVPVLITLAVKFLSECPLALHQLEEENIQLKRRKTDMGETLQWT 347

Query: 335 ----------VITETLRMGNIIIGVMRKAMKDVKIKGYLIPKGWSVLTYFRSVHLDDNHY 394
                     VITETLR+GNII G+MRKA++DV++KG+LIPKGW V  YFRSVHLDD  Y
Sbjct: 348 DYMSLSFTQHVITETLRLGNIIGGIMRKAVRDVEVKGHLIPKGWCVFVYFRSVHLDDTLY 407

Query: 395 DWPYKFNPWRWQDRDLSSCNFTPFGGGQRLCPGLDLARLEASIFLHHFVTQFRWIAEEDR 407
           D PYKFNPWRW+++D+S+ +FTPFGGGQRLCPGLDLARLEASIFLHH VT FRW+AEED 
Sbjct: 408 DEPYKFNPWRWKEKDMSNGSFTPFGGGQRLCPGLDLARLEASIFLHHLVTSFRWVAEEDH 467

[Close]

BLAST of Spg013836 vs. ExPASy Swiss-Prot
Match: Q94IA6 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 OS=Arabidopsis thaliana OX=3702 GN=CYP90D1 PE=1 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 1.2e-126
Identity = 243/441 (55.10%), Postives = 299/441 (67.80%), Query Frame = 0


Query: 35  QLPLGSLGWPFIGETIEFISCAYSDRPETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVS 94
           + P GSLGWP IGETIEF+S AYSDRPE+FMDKRR +YG+VFKSHIFG+ TIVSTDAEV+
Sbjct: 50  KFPHGSLGWPVIGETIEFVSSAYSDRPESFMDKRRLMYGRVFKSHIFGTATIVSTDAEVN 109

Query: 95  KFILQSDAKAFVPSYPKSLTELMGKSSILLINGSLQKRIHGLVGAFFKSSHLKAQITKDM 154
           + +LQSD+ AFVP YPK++ ELMGKSSILLINGSL +R HGLVG+F KS  LKAQI +DM
Sbjct: 110 RAVLQSDSTAFVPFYPKTVRELMGKSSILLINGSLHRRFHGLVGSFLKSPLLKAQIVRDM 169

Query: 155 ECY----------------DGVSQATVSRIHRGAY-----GFASEHSRKQALPIVAGKL- 214
             +                  VS+    ++   A      G   E  +++    ++G + 
Sbjct: 170 HKFLSESMDLWSEDQPVLLQDVSKTVAFKVLAKALISVEKGEDLEELKREFENFISGLMS 229

Query: 215 ---------------AKRKMVKLVKQIIQ-----ERKSSGISPVPRDVMEVLLKDESEQL 274
                          AK+ MVK V++II+      +       + +DV++VLLKD SE L
Sbjct: 230 LPINFPGTQLHRSLQAKKNMVKQVERIIEGKIRKTKNKEEDDVIAKDVVDVLLKDSSEHL 289

Query: 275 TDDLIAVNMIDMMIPGEDSVPILMTLAVKYLSDCPTALHQLT------------------ 334
           T +LIA NMIDMMIPG DSVP+L+TLAVK+LSD P AL+ LT                  
Sbjct: 290 THNLIANNMIDMMIPGHDSVPVLITLAVKFLSDSPAALNLLTEENMKLKSLKELTGEPLY 349

Query: 335 ------------VITETLRMGNIIIGVMRKAMKDVKIKGYLIPKGWSVLTYFRSVHLDDN 394
                       VITETLRMGN+IIGVMRKAMKDV+IKGY+IPKGW  L Y RSVHLD  
Sbjct: 350 WNDYLSLPFTQKVITETLRMGNVIIGVMRKAMKDVEIKGYVIPKGWCFLAYLRSVHLDKL 409

Query: 395 HYDWPYKFNPWRWQDRDLSSCNFTPFGGGQRLCPGLDLARLEASIFLHHFVTQFRWIAEE 404
           +Y+ PYKFNPWRWQ+RD+++ +F+PFGGGQRLCPGLDLARLE S+FLHH VT+FRWIAEE
Sbjct: 410 YYESPYKFNPWRWQERDMNTSSFSPFGGGQRLCPGLDLARLETSVFLHHLVTRFRWIAEE 469

[Close]

BLAST of Spg013836 vs. ExPASy Swiss-Prot
Match: Q9M066 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90C1 OS=Arabidopsis thaliana OX=3702 GN=CYP90C1 PE=1 SV=3)

HSP 1 Score: 375.2 bits (962), Expect = 9.6e-103
Identity = 207/451 (45.90%), Postives = 281/451 (62.31%), Query Frame = 0


Query: 36  LPLGSLGWPFIGETIEFISCAYSDRPETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSK 95
           +P GSLGWP IGET+ FI+C YS RP TFMDKR+ LYGKVFK++I G+P I+STDAEV+K
Sbjct: 69  IPNGSLGWPVIGETLNFIACGYSSRPVTFMDKRKSLYGKVFKTNIIGTPIIISTDAEVNK 128

Query: 96  FILQSDAKAFVPSYPKSLTELMGKSSILLINGSLQKRIHGLVGAFFKSSHLKAQITKDME 155
            +LQ+    FVP+YPKS+TEL+G++SIL ING  QKR+H L+GAF +S HLK +IT+D+E
Sbjct: 129 VVLQNHGNTFVPAYPKSITELLGENSILSINGPHQKRLHTLIGAFLRSPHLKDRITRDIE 188

Query: 156 CYDGVSQATVSRIHRGAYGFASEHSRKQALPIVAGKL----------------------- 215
               ++ A+ +++         +  +K    I+   L                       
Sbjct: 189 ASVVLTLASWAQL---PLVHVQDEIKKMTFEILVKVLMSTSPGEDMNILKLEFEEFIKGL 248

Query: 216 -----------------AKRKMVKLVKQIIQERKSSGISPVP-RDVMEVLLKD----ESE 275
                            AK +++K+VK++++ER+ +  +  P  DV++VLL+D    E +
Sbjct: 249 ICIPIKFPGTRLYKSLKAKERLIKMVKKVVEERQVAMTTTSPANDVVDVLLRDGGDSEKQ 308

Query: 276 QLTDDLIAVNMIDMMIPGEDSVPILMTLAVKYLSDCPTALHQL----------------- 335
               D ++  +++MMIPGE+++P  MTLAVK+LSD P AL +L                 
Sbjct: 309 SQPSDFVSGKIVEMMIPGEETMPTAMTLAVKFLSDNPVALAKLVEENMEMKRRKLELGEE 368

Query: 336 -------------TVITETLRMGNIIIGVMRKAMKDVKIKGYLIPKGWSVLTYFRSVHLD 395
                         VI ETLRM NII GV RKA+KDV+IKGYLIPKGW VL  F SVH+D
Sbjct: 369 YKWTDYMSLSFTQNVINETLRMANIINGVWRKALKDVEIKGYLIPKGWCVLASFISVHMD 428

Query: 396 DNHYDWPYKFNPWRWQDRDLSSCN----FTPFGGGQRLCPGLDLARLEASIFLHHFVTQF 408
           ++ YD PY+F+PWRW DR   S N    FTPFGGGQRLCPGL+L++LE SIFLHH VT++
Sbjct: 429 EDIYDNPYQFDPWRW-DRINGSANSSICFTPFGGGQRLCPGLELSKLEISIFLHHLVTRY 488

[Close]

BLAST of Spg013836 vs. ExPASy Swiss-Prot
Match: Q42569 (Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 1.1e-74
Identity = 168/439 (38.27%), Postives = 240/439 (54.67%), Query Frame = 0


Query: 36  LPLGSLGWPFIGETIEFISCAYSDRPETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSK 95
           LP GSLG P IGET + I    ++ PE F+D+R   YG VF +H+FG PTI S D E ++
Sbjct: 31  LPPGSLGLPLIGETFQLIGAYKTENPEPFIDERVARYGSVFMTHLFGEPTIFSADPETNR 90

Query: 96  FILQSDAKAFVPSYPKSLTELMGKSSILLINGSLQKRIHGLVGAFFKSSHLKAQITKDM- 155
           F+LQ++ K F  SYP S+  L+GK S+LL+ GSL KR+H L  +F  SS +K  +  D+ 
Sbjct: 91  FVLQNEGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMSFANSSIIKDHLMLDID 150

Query: 156 ------------------ECYDGVSQATVSRIHRGAYGFASEHSRKQALPIVAGKL---- 215
                             E      + TV ++     G  SE  RK+ L ++ G      
Sbjct: 151 RLVRFNLDSWSSRVLLMEEAKKITFELTVKQLMSFDPGEWSESLRKEYLLVIEGFFSLPL 210

Query: 216 ------------AKRKMVKLVKQIIQERKSSGISPVPR--DVMEVLLKDESEQLTDDLIA 275
                       A+RK+ + +  ++ +R+        R  D++  LL  + +  +D+ I 
Sbjct: 211 PLFSTTYRKAIQARRKVAEALTVVVMKRREEEEEGAERKKDMLAALLAAD-DGFSDEEIV 270

Query: 276 VNMIDMMIPGEDSVPILMTLAVKYLSDCPTALHQL------------------------- 335
             ++ +++ G ++   +MTLAVK+L++ P AL QL                         
Sbjct: 271 DFLVALLVAGYETTSTIMTLAVKFLTETPLALAQLKEEHEKIRAMKSDSYSLEWSDYKSM 330

Query: 336 ----TVITETLRMGNIIIGVMRKAMKDVKIKGYLIPKGWSVLTYFRSVHLDDNHYDWPYK 395
                V+ ETLR+ NII GV R+AM DV+IKGY IPKGW V + FR+VHLD NH+     
Sbjct: 331 PFTQCVVNETLRVANIIGGVFRRAMTDVEIKGYKIPKGWKVFSSFRAVHLDPNHFKDART 390

Query: 396 FNPWRWQDRDLS---SCNFTPFGGGQRLCPGLDLARLEASIFLHHFVTQFRWI-AEEDRI 405
           FNPWRWQ   ++   S  FTPFGGG RLCPG +LAR+  S+FLH  VT F W+ AE+D++
Sbjct: 391 FNPWRWQSNSVTTGPSNVFTPFGGGPRLCPGYELARVALSVFLHRLVTGFSWVPAEQDKL 450

[Close]

BLAST of Spg013836 vs. ExPASy Swiss-Prot
Match: Q8GSQ1 (Cytochrome P450 85A1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP85A1 PE=1 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 3.2e-58
Identity = 144/470 (30.64%), Postives = 242/470 (51.49%), Query Frame = 0


Query: 7   VVVVTAILLTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDRPETFMD 66
           VVV  A+++++++L  N  R  R +    LP G++GWP  GET EF+      +  +FM 
Sbjct: 8   VVVAAAVVVSSLLLRWNEVRYSRKR---GLPPGTMGWPLFGETTEFLK-----QGPSFMK 67

Query: 67  KRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKSSILLIN 126
            RR  YG VF++HI G PT+V  +AE+++  L S+ + FVP YP+S+ +++G+++I  + 
Sbjct: 68  ARRLRYGSVFRTHILGCPTVVCMEAELNRRALASEGRGFVPGYPQSMLDILGRNNIAAVQ 127

Query: 127 GSLQKRIHGLVGAFFKSSHLKAQITKDMECY--------DGVSQATVSRIHRGAYGFASE 186
           G L + + G + +  + + +++ +   ++ +           S + V  I       A  
Sbjct: 128 GPLHRAMRGAMLSLVRPAMIRSSLLPKIDAFMRSHLAAWSSSSSSAVVDIQAKTKEMALL 187

Query: 187 HSRKQALPIVAGKL--------------------------------AKRKMVKLVKQIIQ 246
            + +Q   + AG L                                A++K+V +++Q+I 
Sbjct: 188 SALRQIAGVSAGPLSDALKAELYTLVLGTISLPINLPGTNYYQGFKARKKLVAMLEQMIA 247

Query: 247 ERKSSGISPVPRDVMEVLL---KDESEQLTDDLIAVNMIDMMIPGEDSVPILMTLAVKYL 306
           ER+SSG   V  D+++ LL   +   E+LTD+ I   +I ++  G +++     +AVKYL
Sbjct: 248 ERRSSG--QVHDDMLDALLTGVEGTREKLTDEQIIDLIITLIYSGYETMSTTSMMAVKYL 307

Query: 307 SDCPTALHQL-----------------------------TVITETLRMGNIIIGVMRKAM 366
           SD P AL QL                              VI ETLR+  ++ G++RK  
Sbjct: 308 SDHPKALEQLRKEHFDIRKGKAPEDAIDWNDFKSMTFTRAVIFETLRLATVVNGLLRKTT 367

Query: 367 KDVKIKGYLIPKGWSVLTYFRSVHLDDNHYDWPYKFNPWRWQDRDLSS-CNFTPFGGGQR 403
           +DV++ GY+IPKGW +  Y R ++ D   Y  P  FNPWRW ++++ S  +F  FGGG R
Sbjct: 368 QDVEMNGYVIPKGWRIYVYTREINYDPFLYPDPMTFNPWRWLEKNMESHPHFMLFGGGSR 427

[Close]

BLAST of Spg013836 vs. ExPASy TrEMBL
Match: A0A0A0K7W8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G451390 PE=3 SV=1)

HSP 1 Score: 678.3 bits (1749), Expect = 2.0e-191
Identity = 359/473 (75.90%), Postives = 375/473 (79.28%), Query Frame = 0


Query: 1   MDSLWDVVVVTAILLTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60
           MDSLWDV+VVTAI  TTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFIS AYSDR
Sbjct: 1   MDSLWDVLVVTAIFFTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISSAYSDR 60

Query: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120
           PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYDGVS------------------- 180
           SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECY  +S                   
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMANWTENNPIYLQDETKNI 180

Query: 181 --QATVSRIHRGAYGFASEHSRKQALPIVAGKL----------------AKRKMVKLVKQ 240
             Q  V  +     G + E  +KQ    +AG +                AKR MVKLVK+
Sbjct: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK 240

Query: 241 IIQERKSSGISPVPRDVMEVLLKDESEQLTDDLIAVNMIDMMIPGEDSVPILMTLAVKYL 300
           IIQERKS GIS VP+D+MEVLLKDE+E+LTDDLIA NMIDMMIPGEDSVPILMTLAVKYL
Sbjct: 241 IIQERKSCGISTVPKDIMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300

Query: 301 SDCPTALHQLT------------------------------VITETLRMGNIIIGVMRKA 360
           SDCP AL QLT                              VITETLRMGNIIIGVMRKA
Sbjct: 301 SDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRKA 360

Query: 361 MKDVKIKGYLIPKGWSVLTYFRSVHLDDNHYDWPYKFNPWRWQDRDLSSCNFTPFGGGQR 407
           MKD+KIKGYLIPKGWSVLTYFRSVHLDDNHYD PYKFNPWRWQDR++SSCNFTPFGGGQR
Sbjct: 361 MKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQDREMSSCNFTPFGGGQR 420

[Close]

BLAST of Spg013836 vs. ExPASy TrEMBL
Match: A0A1S3CGU6 (3-epi-6-deoxocathasterone 23-monooxygenase OS=Cucumis melo OX=3656 GN=LOC103500734 PE=3 SV=1)

HSP 1 Score: 671.8 bits (1732), Expect = 1.8e-189
Identity = 359/473 (75.90%), Postives = 375/473 (79.28%), Query Frame = 0


Query: 1   MDSLWDVVVVTAILLTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60
           MDSLWDV+VVTAI   TIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR
Sbjct: 1   MDSLWDVLVVTAI---TIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60

Query: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120
           PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYDGVSQAT---------------- 180
           SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECY  +S A+                
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMASWTENNPIYLQDETKNI 180

Query: 181 -----VSRIHRGAYGFASEHSRKQALPIVAGKL----------------AKRKMVKLVKQ 240
                V  +     G + E  +KQ    +AG +                AKR MVKLVK+
Sbjct: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK 240

Query: 241 IIQERKSSGISPVPRDVMEVLLKDESEQLTDDLIAVNMIDMMIPGEDSVPILMTLAVKYL 300
           IIQERKS GIS VP+DVMEVLLKDE+E+LTDDLIA NMIDMMIPGEDSVPILMTLAVKYL
Sbjct: 241 IIQERKSCGISTVPKDVMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300

Query: 301 SDCPTALHQLT------------------------------VITETLRMGNIIIGVMRKA 360
           SDCP AL QLT                              VITETLRMGNIIIGVMRKA
Sbjct: 301 SDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRKA 360

Query: 361 MKDVKIKGYLIPKGWSVLTYFRSVHLDDNHYDWPYKFNPWRWQDRDLSSCNFTPFGGGQR 407
           MKD+KIKGYLI KGWSVLTYFRSVHLDDNHYD PYKFNPWRWQ+R+LSSCNFTPFGGGQR
Sbjct: 361 MKDMKIKGYLILKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQERELSSCNFTPFGGGQR 420

[Close]

BLAST of Spg013836 vs. ExPASy TrEMBL
Match: A0A5A7UW93 (Cytochrome p450 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold242G001660 PE=3 SV=1)

HSP 1 Score: 617.5 bits (1591), Expect = 4.1e-173
Identity = 337/470 (71.70%), Postives = 356/470 (75.74%), Query Frame = 0


Query: 1   MDSLWDVVVVTAILLTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60
           MDSLWDV+VVTAI   TIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR
Sbjct: 1   MDSLWDVLVVTAI---TIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60

Query: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120
           PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYDGVSQAT---------------- 180
           SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECY  +S A+                
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMASWTENNPIYLQDETKNI 180

Query: 181 -----VSRIHRGAYGFASEHSRKQALPIVAGKL--------------------AKRKMVK 240
                V  +     G + E  +KQ    +AG +                    AKR MVK
Sbjct: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQASWTAKRNMVK 240

Query: 241 LVKQIIQERKSSGISPVPRDVMEVLLKDESEQLTDDLIAVNMIDMMIPGEDSVPILMTLA 300
           LVK+IIQERKS GIS VP+DVMEVLLKDE+E+LTDDLIA NMIDMMIPGEDSVPILMTLA
Sbjct: 241 LVKKIIQERKSCGISTVPKDVMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLA 300

Query: 301 VKYLSDCPTALHQLT------------------------------VITETLRMGNIIIGV 360
           VKYLSDCP AL QLT                              VITETLRMGNIIIGV
Sbjct: 301 VKYLSDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGV 360

Query: 361 MRKAMKDVKIKGYLIPKGWSVLTYFRSVHLDDNHYDWPYKFNPWRWQDRDLSSCNFTPFG 400
           MRKAMKD+KIKGYLIPKGWSVLTYFRSVHLDDNHYD PYKFNPWRWQ+R+LSSCNFTPFG
Sbjct: 361 MRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQERELSSCNFTPFG 420

[Close]

BLAST of Spg013836 vs. ExPASy TrEMBL
Match: E5GCA5 (Cytochrome p450 OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 615.1 bits (1585), Expect = 2.0e-172
Identity = 336/466 (72.10%), Postives = 355/466 (76.18%), Query Frame = 0


Query: 1   MDSLWDVVVVTAILLTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60
           MDSLWDV+VVTAI   TIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR
Sbjct: 1   MDSLWDVLVVTAI---TIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60

Query: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120
           PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYDGVSQAT---------------- 180
           SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECY  +S A+                
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMASWTENNPIYLQDETKNI 180

Query: 181 -----VSRIHRGAYGFASEHSRKQALPIVAGKL----------------AKRKMVKLVKQ 240
                V  +     G + E  +KQ    +AG +                AKR MVKLVK+
Sbjct: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK 240

Query: 241 IIQERKSSGISPVPRDVMEVLLKDESEQLTDDLIAVNMIDMMIPGEDSVPILMTLAVKYL 300
           IIQERKS GIS VP+DVMEVLLKDE+E+LTDDLIA NMIDMMIPGEDSVPILMTLAVKYL
Sbjct: 241 IIQERKSCGISTVPKDVMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300

Query: 301 SDCPTALHQLT------------------------------VITETLRMGNIIIGVMRKA 360
           SDCP AL QLT                              VITETLRMGNIIIGVMRKA
Sbjct: 301 SDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRKA 360

Query: 361 MKDVKIKGYLIPKGWSVLTYFRSVHLDDNHYDWPYKFNPWRWQDRDLSSCNFTPFGGGQR 400
           MKD+KIKGYLI KGWSVLTYFRSVHLDDNHYD PYKFNPWRWQ+R+LSSCNFTPFGGGQR
Sbjct: 361 MKDMKIKGYLILKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQERELSSCNFTPFGGGQR 420

[Close]

BLAST of Spg013836 vs. ExPASy TrEMBL
Match: A0A5N6QIE4 (Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_003593 PE=3 SV=1)

HSP 1 Score: 595.1 bits (1533), Expect = 2.2e-166
Identity = 312/437 (71.40%), Postives = 344/437 (78.72%), Query Frame = 0


Query: 1   MDSLWDVVVVTAILLTTIILYR-NCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSD 60
           MD++W VV+ TAI L+ IILYR N FRL+RSK  + LPLG+LG PF+GETIEF+S AYSD
Sbjct: 1   MDNMW-VVLGTAIFLSAIILYRKNRFRLIRSKHRSPLPLGTLGLPFLGETIEFVSFAYSD 60

Query: 61  RPETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGK 120
           RPETFMDKRRR+YGKVFKSHIFGSPTIVSTDAEVSKF+LQSDAK FVPSYPKSLTELMGK
Sbjct: 61  RPETFMDKRRRIYGKVFKSHIFGSPTIVSTDAEVSKFVLQSDAKTFVPSYPKSLTELMGK 120

Query: 121 SSILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYDGVSQATVSRIHRGAYGFASEH 180
           SSILLINGSLQKRIHGL+GAFFKS HLKAQIT+DM  Y   S A                
Sbjct: 121 SSILLINGSLQKRIHGLIGAFFKSPHLKAQITRDMHKYVQESLA---------------- 180

Query: 181 SRKQALPIVAGKLAKRKMVKLVKQIIQERKSSGISPVPRDVMEVLLKDESEQLTDDLIAV 240
           + K   PI      K     LV+ IIQ R+ SG+S VP+DV++VLL D SEQLTDDLIA 
Sbjct: 181 NWKDDTPIYIQDETK----NLVQNIIQARRHSGLSTVPKDVVDVLLNDSSEQLTDDLIAD 240

Query: 241 NMIDMMIPGEDSVPILMTLAVKYLSDCPTALHQL-------------------------- 300
           N IDMMIPGEDSVP+LMTLA+KYLSDCPTAL QL                          
Sbjct: 241 NTIDMMIPGEDSVPVLMTLAIKYLSDCPTALQQLTEENLKMKKLKQQLGEPLSWSDYLSL 300

Query: 301 ----TVITETLRMGNIIIGVMRKAMKDVKIKGYLIPKGWSVLTYFRSVHLDDNHYDWPYK 360
               TVITETLRMGNIIIGVMRKAMKDV+IKGYLIPKGW V TYFRSVHLD+NHY+WPYK
Sbjct: 301 PFTQTVITETLRMGNIIIGVMRKAMKDVEIKGYLIPKGWCVFTYFRSVHLDENHYEWPYK 360

Query: 361 FNPWRWQDRDLSSCNFTPFGGGQRLCPGLDLARLEASIFLHHFVTQFRWIAEEDRIINFP 407
           FNPWRWQD+D+SSC+FTPFGGGQRLCPGLDLARLEASIFLHHFVTQFRW+AEED I+NFP
Sbjct: 361 FNPWRWQDKDMSSCSFTPFGGGQRLCPGLDLARLEASIFLHHFVTQFRWVAEEDTIVNFP 416

[Close]

BLAST of Spg013836 vs. TAIR 10
Match: AT3G13730.1 (cytochrome P450, family 90, subfamily D, polypeptide 1 )

HSP 1 Score: 454.5 bits (1168), Expect = 8.8e-128
Identity = 243/441 (55.10%), Postives = 299/441 (67.80%), Query Frame = 0


Query: 35  QLPLGSLGWPFIGETIEFISCAYSDRPETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVS 94
           + P GSLGWP IGETIEF+S AYSDRPE+FMDKRR +YG+VFKSHIFG+ TIVSTDAEV+
Sbjct: 50  KFPHGSLGWPVIGETIEFVSSAYSDRPESFMDKRRLMYGRVFKSHIFGTATIVSTDAEVN 109

Query: 95  KFILQSDAKAFVPSYPKSLTELMGKSSILLINGSLQKRIHGLVGAFFKSSHLKAQITKDM 154
           + +LQSD+ AFVP YPK++ ELMGKSSILLINGSL +R HGLVG+F KS  LKAQI +DM
Sbjct: 110 RAVLQSDSTAFVPFYPKTVRELMGKSSILLINGSLHRRFHGLVGSFLKSPLLKAQIVRDM 169

Query: 155 ECY----------------DGVSQATVSRIHRGAY-----GFASEHSRKQALPIVAGKL- 214
             +                  VS+    ++   A      G   E  +++    ++G + 
Sbjct: 170 HKFLSESMDLWSEDQPVLLQDVSKTVAFKVLAKALISVEKGEDLEELKREFENFISGLMS 229

Query: 215 ---------------AKRKMVKLVKQIIQ-----ERKSSGISPVPRDVMEVLLKDESEQL 274
                          AK+ MVK V++II+      +       + +DV++VLLKD SE L
Sbjct: 230 LPINFPGTQLHRSLQAKKNMVKQVERIIEGKIRKTKNKEEDDVIAKDVVDVLLKDSSEHL 289

Query: 275 TDDLIAVNMIDMMIPGEDSVPILMTLAVKYLSDCPTALHQLT------------------ 334
           T +LIA NMIDMMIPG DSVP+L+TLAVK+LSD P AL+ LT                  
Sbjct: 290 THNLIANNMIDMMIPGHDSVPVLITLAVKFLSDSPAALNLLTEENMKLKSLKELTGEPLY 349

Query: 335 ------------VITETLRMGNIIIGVMRKAMKDVKIKGYLIPKGWSVLTYFRSVHLDDN 394
                       VITETLRMGN+IIGVMRKAMKDV+IKGY+IPKGW  L Y RSVHLD  
Sbjct: 350 WNDYLSLPFTQKVITETLRMGNVIIGVMRKAMKDVEIKGYVIPKGWCFLAYLRSVHLDKL 409

Query: 395 HYDWPYKFNPWRWQDRDLSSCNFTPFGGGQRLCPGLDLARLEASIFLHHFVTQFRWIAEE 404
           +Y+ PYKFNPWRWQ+RD+++ +F+PFGGGQRLCPGLDLARLE S+FLHH VT+FRWIAEE
Sbjct: 410 YYESPYKFNPWRWQERDMNTSSFSPFGGGQRLCPGLDLARLETSVFLHHLVTRFRWIAEE 469

[Close]

BLAST of Spg013836 vs. TAIR 10
Match: AT4G36380.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 375.2 bits (962), Expect = 6.8e-104
Identity = 207/451 (45.90%), Postives = 281/451 (62.31%), Query Frame = 0


Query: 36  LPLGSLGWPFIGETIEFISCAYSDRPETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSK 95
           +P GSLGWP IGET+ FI+C YS RP TFMDKR+ LYGKVFK++I G+P I+STDAEV+K
Sbjct: 69  IPNGSLGWPVIGETLNFIACGYSSRPVTFMDKRKSLYGKVFKTNIIGTPIIISTDAEVNK 128

Query: 96  FILQSDAKAFVPSYPKSLTELMGKSSILLINGSLQKRIHGLVGAFFKSSHLKAQITKDME 155
            +LQ+    FVP+YPKS+TEL+G++SIL ING  QKR+H L+GAF +S HLK +IT+D+E
Sbjct: 129 VVLQNHGNTFVPAYPKSITELLGENSILSINGPHQKRLHTLIGAFLRSPHLKDRITRDIE 188

Query: 156 CYDGVSQATVSRIHRGAYGFASEHSRKQALPIVAGKL----------------------- 215
               ++ A+ +++         +  +K    I+   L                       
Sbjct: 189 ASVVLTLASWAQL---PLVHVQDEIKKMTFEILVKVLMSTSPGEDMNILKLEFEEFIKGL 248

Query: 216 -----------------AKRKMVKLVKQIIQERKSSGISPVP-RDVMEVLLKD----ESE 275
                            AK +++K+VK++++ER+ +  +  P  DV++VLL+D    E +
Sbjct: 249 ICIPIKFPGTRLYKSLKAKERLIKMVKKVVEERQVAMTTTSPANDVVDVLLRDGGDSEKQ 308

Query: 276 QLTDDLIAVNMIDMMIPGEDSVPILMTLAVKYLSDCPTALHQL----------------- 335
               D ++  +++MMIPGE+++P  MTLAVK+LSD P AL +L                 
Sbjct: 309 SQPSDFVSGKIVEMMIPGEETMPTAMTLAVKFLSDNPVALAKLVEENMEMKRRKLELGEE 368

Query: 336 -------------TVITETLRMGNIIIGVMRKAMKDVKIKGYLIPKGWSVLTYFRSVHLD 395
                         VI ETLRM NII GV RKA+KDV+IKGYLIPKGW VL  F SVH+D
Sbjct: 369 YKWTDYMSLSFTQNVINETLRMANIINGVWRKALKDVEIKGYLIPKGWCVLASFISVHMD 428

Query: 396 DNHYDWPYKFNPWRWQDRDLSSCN----FTPFGGGQRLCPGLDLARLEASIFLHHFVTQF 408
           ++ YD PY+F+PWRW DR   S N    FTPFGGGQRLCPGL+L++LE SIFLHH VT++
Sbjct: 429 EDIYDNPYQFDPWRW-DRINGSANSSICFTPFGGGQRLCPGLELSKLEISIFLHHLVTRY 488

[Close]

BLAST of Spg013836 vs. TAIR 10
Match: AT5G05690.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 282.0 bits (720), Expect = 7.8e-76
Identity = 168/439 (38.27%), Postives = 240/439 (54.67%), Query Frame = 0


Query: 36  LPLGSLGWPFIGETIEFISCAYSDRPETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSK 95
           LP GSLG P IGET + I    ++ PE F+D+R   YG VF +H+FG PTI S D E ++
Sbjct: 31  LPPGSLGLPLIGETFQLIGAYKTENPEPFIDERVARYGSVFMTHLFGEPTIFSADPETNR 90

Query: 96  FILQSDAKAFVPSYPKSLTELMGKSSILLINGSLQKRIHGLVGAFFKSSHLKAQITKDM- 155
           F+LQ++ K F  SYP S+  L+GK S+LL+ GSL KR+H L  +F  SS +K  +  D+ 
Sbjct: 91  FVLQNEGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMSFANSSIIKDHLMLDID 150

Query: 156 ------------------ECYDGVSQATVSRIHRGAYGFASEHSRKQALPIVAGKL---- 215
                             E      + TV ++     G  SE  RK+ L ++ G      
Sbjct: 151 RLVRFNLDSWSSRVLLMEEAKKITFELTVKQLMSFDPGEWSESLRKEYLLVIEGFFSLPL 210

Query: 216 ------------AKRKMVKLVKQIIQERKSSGISPVPR--DVMEVLLKDESEQLTDDLIA 275
                       A+RK+ + +  ++ +R+        R  D++  LL  + +  +D+ I 
Sbjct: 211 PLFSTTYRKAIQARRKVAEALTVVVMKRREEEEEGAERKKDMLAALLAAD-DGFSDEEIV 270

Query: 276 VNMIDMMIPGEDSVPILMTLAVKYLSDCPTALHQL------------------------- 335
             ++ +++ G ++   +MTLAVK+L++ P AL QL                         
Sbjct: 271 DFLVALLVAGYETTSTIMTLAVKFLTETPLALAQLKEEHEKIRAMKSDSYSLEWSDYKSM 330

Query: 336 ----TVITETLRMGNIIIGVMRKAMKDVKIKGYLIPKGWSVLTYFRSVHLDDNHYDWPYK 395
                V+ ETLR+ NII GV R+AM DV+IKGY IPKGW V + FR+VHLD NH+     
Sbjct: 331 PFTQCVVNETLRVANIIGGVFRRAMTDVEIKGYKIPKGWKVFSSFRAVHLDPNHFKDART 390

Query: 396 FNPWRWQDRDLS---SCNFTPFGGGQRLCPGLDLARLEASIFLHHFVTQFRWI-AEEDRI 405
           FNPWRWQ   ++   S  FTPFGGG RLCPG +LAR+  S+FLH  VT F W+ AE+D++
Sbjct: 391 FNPWRWQSNSVTTGPSNVFTPFGGGPRLCPGYELARVALSVFLHRLVTGFSWVPAEQDKL 450

[Close]

BLAST of Spg013836 vs. TAIR 10
Match: AT3G50660.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 215.7 bits (548), Expect = 6.9e-56
Identity = 149/498 (29.92%), Postives = 245/498 (49.20%), Query Frame = 0


Query: 12  AILLTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDRPETFMDKRRRL 71
           ++LL  I+L R   R  +++F   LP G  GWPF+GETI ++    +     FM +    
Sbjct: 20  SLLLFLILLKR---RNRKTRF--NLPPGKSGWPFLGETIGYLKPYTATTLGDFMQQHVSK 79

Query: 72  YGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKSSILLINGSLQK 131
           YGK+++S++FG PTIVS DA +++FILQ++ + F  SYP+S+  ++GK S+L++ G + +
Sbjct: 80  YGKIYRSNLFGEPTIVSADAGLNRFILQNEGRLFECSYPRSIGGILGKWSMLVLVGDMHR 139

Query: 132 RIHGLVGAFFKSSHLKAQITKDMECY-----DGVSQATVSRIHRGAYGFA---------- 191
            +  +   F   + L+  + KD+E +     D   Q ++      A  F           
Sbjct: 140 DMRSISLNFLSHARLRTILLKDVERHTLFVLDSWQQNSIFSAQDEAKKFTFNLMAKHIMS 199

Query: 192 -------SEHSRKQALPIVAGKL----------------AKRKMVKLVKQIIQERKSSGI 251
                  +E  +K+ +  + G +                ++  ++K +++ ++ERK   I
Sbjct: 200 MDPGEEETEQLKKEYVTFMKGVVSAPLNLPGTAYHKALQSRATILKFIERKMEERKLD-I 259

Query: 252 SPVPRDVMEVLLKDESE---------QLTDD-----------LIAVNMIDMMI----PGE 311
               ++  EV  +DE+E         Q TDD           L    ++D+++     G 
Sbjct: 260 KEEDQEEEEVKTEDEAEMSKSDHVRKQRTDDDLLGWVLKHSNLSTEQILDLILSLLFAGH 319

Query: 312 DSVPILMTLAVKYLSDCPTALHQL-------------------------------TVITE 371
           ++  + + LA+ +L  CP A+ +L                                VI E
Sbjct: 320 ETSSVAIALAIFFLQACPKAVEELREEHLEIARAKKELGESELNWDDYKKMDFTQCVINE 379

Query: 372 TLRMGNIIIGVMRKAMKDVKIKGYLIPKGWSVLTYFRSVHLDDNHYDWPYKFNPWRWQDR 404
           TLR+GN++  + RKA+KDV+ KGY IP GW VL    +VHLD++ YD P  FNPWRWQ +
Sbjct: 380 TLRLGNVVRFLHRKALKDVRYKGYDIPSGWKVLPVISAVHLDNSRYDQPNLFNPWRWQQQ 439

[Close]

BLAST of Spg013836 vs. TAIR 10
Match: AT5G38970.1 (brassinosteroid-6-oxidase 1 )

HSP 1 Score: 213.8 bits (543), Expect = 2.6e-55
Identity = 144/458 (31.44%), Postives = 229/458 (50.00%), Query Frame = 0


Query: 7   VVVVTAILLTTIILYRNCFRLLRSKFC-NQLPLGSLGWPFIGETIEFISCAYSDRPETFM 66
           ++V+  +LL  + L     R  + ++  N LP G++GWP  GET EF+      +   FM
Sbjct: 4   MMVMMGLLLIIVSLCSALLRWNQMRYTKNGLPPGTMGWPIFGETTEFLK-----QGPNFM 63

Query: 67  DKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKSSILLI 126
             +R  YG  FKSH+ G PT++S D+EV+++IL++++K  VP YP+S+ +++G  ++  +
Sbjct: 64  RNQRLRYGSFFKSHLLGCPTLISMDSEVNRYILKNESKGLVPGYPQSMLDILGTCNMAAV 123

Query: 127 NGSLQKRIHG----LVGAFFKSSHLKAQITKDMECY-DGVSQATVSRIHRGAYGFA---- 186
           +GS  + + G    L+ +     H+  ++   M  Y D  ++  V  I       A    
Sbjct: 124 HGSSHRLMRGSLLSLISSTMMRDHILPKVDHFMRSYLDQWNELEVIDIQDKTKHMAFLSS 183

Query: 187 ------------SEHSRKQALPIVAGKL----------------AKRKMVKLVKQIIQER 246
                        E  +     +V G L                A+  + +L+++++QER
Sbjct: 184 LTQIAGNLRKPFVEEFKTAFFKLVVGTLSVPIDLPGTNYRCGIQARNNIDRLLRELMQER 243

Query: 247 KSSGISPVPRDVMEVLLKDESEQ--LTDDLIAVNMIDMMIPGEDSVPILMTLAVKYLSDC 306
           + SG      D++  L+K E  +  LTD+ I   ++ ++  G ++V     +A+KYL D 
Sbjct: 244 RDSG--ETFTDMLGYLMKKEGNRYPLTDEEIRDQVVTILYSGYETVSTTSMMALKYLHDH 303

Query: 307 PTALHQL-----------------------------TVITETLRMGNIIIGVMRKAMKDV 366
           P AL +L                              VI ET R+  I+ GV+RK  +D+
Sbjct: 304 PKALQELRAEHLAFRERKRQDEPLGLEDVKSMKFTRAVIYETSRLATIVNGVLRKTTRDL 363

Query: 367 KIKGYLIPKGWSVLTYFRSVHLDDNHYDWPYKFNPWRWQDRDLSSCNFT-PFGGGQRLCP 392
           +I GYLIPKGW +  Y R ++ D N Y+ P  FNPWRW  + L S N    FGGG RLCP
Sbjct: 364 EINGYLIPKGWRIYVYTREINYDANLYEDPLIFNPWRWMKKSLESQNSCFVFGGGTRLCP 423

The following BLAST results are available for this feature:

BLAST of Spg013836 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008462361.1	3.8e-189	75.90	PREDICTED: 3-epi-6-deoxocathasterone 23-monooxygenase [Cucumis melo]	[more]
KAG6575481.1	8.7e-178	68.06	3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1, partial [Cucurbita argyrospe...	[more]
XP_038898401.1	6.3e-176	73.48	LOW QUALITY PROTEIN: cytochrome P450 90D2-like [Benincasa hispida]	[more]
XP_011659645.1	4.5e-174	72.10	LOW QUALITY PROTEIN: 3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 [Cucumis...	[more]
KAE8646515.1	2.2e-173	74.55	hypothetical protein Csa_016429 [Cucumis sativus]	[more]

BLAST of Spg013836 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94IW5	5.4e-130	55.43	Cytochrome P450 90D2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90D2 PE=1 SV...	[more]
Q94IA6	1.2e-126	55.10	3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 OS=Arabidopsis thaliana OX=37...	[more]
Q9M066	9.6e-103	45.90	3-epi-6-deoxocathasterone 23-monooxygenase CYP90C1 OS=Arabidopsis thaliana OX=37...	[more]
Q42569	1.1e-74	38.27	Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1	[more]
Q8GSQ1	3.2e-58	30.64	Cytochrome P450 85A1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP85A1 PE=1 SV...	[more]

BLAST of Spg013836 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K7W8	2.0e-191	75.90	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G451390 PE=3 SV=1	[more]
A0A1S3CGU6	1.8e-189	75.90	3-epi-6-deoxocathasterone 23-monooxygenase OS=Cucumis melo OX=3656 GN=LOC1035007...	[more]
A0A5A7UW93	4.1e-173	71.70	Cytochrome p450 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold242G0016...	[more]
E5GCA5	2.0e-172	72.10	Cytochrome p450 OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1	[more]
A0A5N6QIE4	2.2e-166	71.40	Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_003593 PE=3 SV=1	[more]

BLAST of Spg013836 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G13730.1	8.8e-128	55.10	cytochrome P450, family 90, subfamily D, polypeptide 1	[more]
AT4G36380.1	6.8e-104	45.90	Cytochrome P450 superfamily protein	[more]
AT5G05690.1	7.8e-76	38.27	Cytochrome P450 superfamily protein	[more]
AT3G50660.1	6.9e-56	29.92	Cytochrome P450 superfamily protein	[more]
AT5G38970.1	2.6e-55	31.44	brassinosteroid-6-oxidase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 345..355 score: 51.88 coord: 313..337 score: 26.33 coord: 355..378 score: 32.35 coord: 69..88 score: 25.43
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 275..383 e-value: 3.6E-31 score: 108.4
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 30..404 e-value: 6.5E-67 score: 228.2
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 35..404
None	No IPR available	PANTHER	PTHR24286:SF30	3-EPI-6-DEOXOCATHASTERONE 23-MONOOXYGENASE CYP90D1	coord: 275..399 coord: 15..158
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 192..274
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 275..399 coord: 15..158
None	No IPR available	PANTHER	PTHR24286:SF30	3-EPI-6-DEOXOCATHASTERONE 23-MONOOXYGENASE CYP90D1	coord: 192..274
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 348..357

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg013836.1	Spg013836.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016132	brassinosteroid biosynthetic process
biological_process	GO:0010268	brassinosteroid homeostasis
biological_process	GO:0016125	sterol metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016709	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen