Spg013797 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg013797
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionAuxin-responsive GH3 family protein
Locationscaffold3: 36564365 .. 36567729 (-)
RNA-Seq ExpressionSpg013797
SyntenySpg013797
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTGGAGGGAAGTAGCCACTGGAGTTTCCTCTTACAAAAATTCCTCAGCTCCCCGTTCTTTTCTCCTATAAATCATCCCACTCAGTTTCACTTTTCACTCAGATTCTCAAACCCATTTCTCTTCTTCCTTTTTCCCTCAATCATGTTCCTCAAATTCTTCAACCAATCCCCAAATTCCTCGTTTCAATTTCCCTTCTGATTCCTCTTCAAATTCCTTGTTTCTTCGTTTCGCCTTGCTCTTCCTGGTAATTTCTCTACTTCCCTCTCTAATTTCTGAGTATGAGCTTTTCCCCTTCAATTTCCTTTTGCAATTCGAAAAACATGTTCTGGGTGTTGATTTTTCCATCGATTCATTTCTGGGTTTTGCTGCTCTGCTTTCAATTATTAGTGAATAGTGTTTTTCTTTTTCAATTCCTGTAATCGATGTTCTGGTTCATGCTCGCACATTATTGAATTAGTGTTAAACAAGTGTCATTCTCAGAATAGCTAGCTGTTGTTTCTTGGACTTTTCTCCTCTGTTTGTTTCTGGAATTCGGATCTTAAAAGTTTCTTTTACTAATTTTATATTCTTCCCTCTGTTTGGTTGCTCAGAAAAGAGAAAAAAGGAAGAGGAAATTTTATCCAACTCTGATTCCTAAATAGTTGACTTTGACGTTTTTTTTTGTATTCTTTGGCATCTTAGCCCAGTTCAATTATGCCTTGAGATTGATTGCTGTTAGTTTTGATTACTGTTTTAAGTTATATATATATATTGATCTTCATTTGCTCTGCACAAAAAAAAAAAAAAAAATAGTTGGGTTTGTGAGGATGTTAGAGAAGATGGAGGAGTTTGATATGGAGAAGGTGATTGAGGAATTTGAGGAATTGACTAAAGATGCTGAGAGAGTTCAAAGAGAGACTTTGAAAATGATTTTGGAAGAAAATGGATCTGCTGAGTATTTGCAGAATTTGGGGCTTAATGGAAAAACGGATCCTGTGAGTTTCAAGGCTTGTGTTCCCATTGTAACACATGAGGACTTGGAGCCTTATATTCAGAGAATTGCTGACGGTCCTTGTGCTCCGATTCTTACCGGAAAACCGATCACTACGATCTCGTTAAGGTAGTTTAGTCTGGTTGCTTTAGTTTTAACCTTGTTTGAATATCCTTGAATGGAAGATTGAGATTGTTTTGGTGTATGTTGTAGTTCTGGTACTACTCAGGGGAAACCAAAGTTCGTCCCATTCAATGATGAGTTGATTGAAACTACAATACAGATATTTCGGACTTCTTTCGCGTTTAGAAACCGGTATGAAACAATTTGGTACTTTTACAGTTTGGACTATGTTGAGTGATTCAATCATGTTGTGTGATTTGTTGAAGAGGATTCATCTGTTTGTGCAGAGATTTTCCAATTGAGAAAGGGAGGTCCTTGATGTTTATTTATGGCAGCAAGCAGTTCAAAACCAATGGAGATTTAATGGCTGCTACTGCCACGAGTAACCTGTACCGCAATCCGAGATTTAAGAGCACAATGAAAGCAATCATGTCCACGACCTGCAGCCCAGATGAAGTCATGTTTGGTTCTGATTTCCATCAATCACTGTATTGTCATCTGCTGTGTGGGCTTATTTTTAGGGATGAAATTCAGATGATATTCTCGACGTTTGCTCATAGCATCGTTCATGCGTTTAGAACGTTTGAAAACATCTGGGAAGAGCTATGTTGTGACATTCAAAGTGGTGTTCTCTCCAGCCGAATCACGTCACCTTCCATTCGTGCAGGGATGTCGAAATTGCTGAAACCGAATCCCGAACTGGCGAATTTGGTGTACGAAAAGTGTGTTGGATTGAGTAATTGGTATGGATTGATACCCCAACTCTTCCCTAACGCAAAGTACATATATGGAATCATGACTGGATCGATGGAACCATATGTGAAAAAGTTGAGACACTATGCAGGAGAGCTGCCTTTGATGAGTGCTGATTATGGTTCATCCGAAGGATGGATTGGTGCAAATACAAACCCGAAAATGTCTCCCGAATTCGCTACTTTCACTGTGCTTCCCAATATTGGTTACTTTGAATTCATTCCTCTCGGGGACGACATCTTCGAGCACGATAAGACTCAATCTTCTTCAATCTGCAACGAGTTGAAACCGGTGGATCTTACTCAAGTCCAAGTTGGTAAAGAGTATGAGATTCTAATCACAAGTGTTGCAGGTACTTCTGTCTTGATTGTTTGCCAATCCATTATACTTTTGTTGTTATTTTACCTCACTTTCAAGATGTCCCATAACTAGTTTTAGTAACCAAAAGAGAAGGATTCCAGGTCTGTATAGGTATAGGCTTGGAGATGTGGTTAAGGTCACAGGCTTCCATAACTCGACCCCGGTACTCAAATTCGTCTGTCGGAGAAACCTCCTGCTAACGATCAACATCGACAAGAACACGGAGAAAGACTTGCAGATAGCTGTTGAGGAAGCCTCAAAGCTACTAGCAGAGGAGAAACTCGAAGTTGTCGATTTCACGAGCTACGTGGATCGATCTACAGAGCCTGGCCACTACGTCATCTTCTGGGAGATGAGTGGTGAAGCTTATAACGACGACATTTTGAAGCAATGCTGCAACTGTTTGGATAGTTCCTTTGTTGATGCAGGCTATGTTAGCTCACGTAGAGTCAAGGCAATTGGCGCGTTGGAACTTCGGGTGGTCCAGAGAGGAACATTCCAGAAGATTCTGGATCACTTCCTGTCGTTAGGGGCGGCGGTGAGTCAGTTTAAAACGCCTCGATGCATCGGTCCAGCCAACAATAAGGTGCTGCAGATCTTGTGCAGTAATGTCAACAAGGTCTACTTCAGTACTGCTTTTCAGTAAGAGCAGCAAGAGAGCTGGAGAAAGAAATGCTATCCTTTTCCCTCAAAACAGTCATAACATTAATCAAACTGTAAAGTTACAAAAAGAAAAAAAGGTTAATTTTTATAGTTTCAATGATGCCCTCAAATATATAAATTGTTTTAAAGATGCCCTTAAGTTTGTTTAGGAGAAATGATTCTTCATTTCTTTTTTCCAAACAGAATTAGGAATACAAATATTTTAAAAATAATTGATAAATTTGAGGGCATTTTTGAAATGTTAAAACTTTAAGATTAAACTGTACTTCAAGGTCATAAAGTAAGGTTTAGGTAGAAAGAAGGAAAGATAGTAAAAAAAAAAAAAAAAAGTGCTGCAGTTAGATTCAATTCAGGCTGTACATGAATGTATTCAGATGTCAAATCCCTTTGTGACAAATGAAAAGGTTAAAATATGCCATTAGCTCTTTGTACTTTATCTTTTATAATGCATTGGTTCTCATCTCATATACATGAATATAATGATTTTGTCTTTACC

mRNA sequence

ATGTTAGAGAAGATGGAGGAGTTTGATATGGAGAAGGTGATTGAGGAATTTGAGGAATTGACTAAAGATGCTGAGAGAGTTCAAAGAGAGACTTTGAAAATGATTTTGGAAGAAAATGGATCTGCTGAGTATTTGCAGAATTTGGGGCTTAATGGAAAAACGGATCCTGTGAGTTTCAAGGCTTGTGTTCCCATTGTAACACATGAGGACTTGGAGCCTTATATTCAGAGAATTGCTGACGGTCCTTGTGCTCCGATTCTTACCGGAAAACCGATCACTACGATCTCGTTAAGTTCTGGTACTACTCAGGGGAAACCAAAGTTCGTCCCATTCAATGATGAGTTGATTGAAACTACAATACAGATATTTCGGACTTCTTTCGCGTTTAGAAACCGAGATTTTCCAATTGAGAAAGGGAGGTCCTTGATGTTTATTTATGGCAGCAAGCAGTTCAAAACCAATGGAGATTTAATGGCTGCTACTGCCACGAGTAACCTGTACCGCAATCCGAGATTTAAGAGCACAATGAAAGCAATCATGTCCACGACCTGCAGCCCAGATGAAGTCATGTTTGGTTCTGATTTCCATCAATCACTGTATTGTCATCTGCTGTGTGGGCTTATTTTTAGGGATGAAATTCAGATGATATTCTCGACGTTTGCTCATAGCATCGTTCATGCGTTTAGAACGTTTGAAAACATCTGGGAAGAGCTATGTTGTGACATTCAAAGTGGTGTTCTCTCCAGCCGAATCACGTCACCTTCCATTCGTGCAGGGATGTCGAAATTGCTGAAACCGAATCCCGAACTGGCGAATTTGGTGTACGAAAAGTGTGTTGGATTGAGTAATTGGTATGGATTGATACCCCAACTCTTCCCTAACGCAAAGTACATATATGGAATCATGACTGGATCGATGGAACCATATGTGAAAAAGTTGAGACACTATGCAGGAGAGCTGCCTTTGATGAGTGCTGATTATGGTTCATCCGAAGGATGGATTGGTGCAAATACAAACCCGAAAATGTCTCCCGAATTCGCTACTTTCACTGTGCTTCCCAATATTGGTTACTTTGAATTCATTCCTCTCGGGGACGACATCTTCGAGCACGATAAGACTCAATCTTCTTCAATCTGCAACGAGTTGAAACCGGTGGATCTTACTCAAGTCCAAGTTGGTAAAGAGTATGAGATTCTAATCACAAGTGTTGCAGGTCTGTATAGGTATAGGCTTGGAGATGTGGTTAAGGTCACAGGCTTCCATAACTCGACCCCGGTACTCAAATTCGTCTGTCGGAGAAACCTCCTGCTAACGATCAACATCGACAAGAACACGGAGAAAGACTTGCAGATAGCTGTTGAGGAAGCCTCAAAGCTACTAGCAGAGGAGAAACTCGAAGTTGTCGATTTCACGAGCTACGTGGATCGATCTACAGAGCCTGGCCACTACGTCATCTTCTGGGAGATGAGTGGTGAAGCTTATAACGACGACATTTTGAAGCAATGCTGCAACTGTTTGGATAGTTCCTTTGTTGATGCAGGCTATGTTAGCTCACGTAGAGTCAAGGCAATTGGCGCGTTGGAACTTCGGGTGGTCCAGAGAGGAACATTCCAGAAGATTCTGGATCACTTCCTGTCGTTAGGGGCGGCGGTGAGTCAGTTTAAAACGCCTCGATGCATCGGTCCAGCCAACAATAAGGTGCTGCAGATCTTGTGCAGTAATGTCAACAAGGTCTACTTCAGTACTGCTTTTCAGTAA

Coding sequence (CDS)

ATGTTAGAGAAGATGGAGGAGTTTGATATGGAGAAGGTGATTGAGGAATTTGAGGAATTGACTAAAGATGCTGAGAGAGTTCAAAGAGAGACTTTGAAAATGATTTTGGAAGAAAATGGATCTGCTGAGTATTTGCAGAATTTGGGGCTTAATGGAAAAACGGATCCTGTGAGTTTCAAGGCTTGTGTTCCCATTGTAACACATGAGGACTTGGAGCCTTATATTCAGAGAATTGCTGACGGTCCTTGTGCTCCGATTCTTACCGGAAAACCGATCACTACGATCTCGTTAAGTTCTGGTACTACTCAGGGGAAACCAAAGTTCGTCCCATTCAATGATGAGTTGATTGAAACTACAATACAGATATTTCGGACTTCTTTCGCGTTTAGAAACCGAGATTTTCCAATTGAGAAAGGGAGGTCCTTGATGTTTATTTATGGCAGCAAGCAGTTCAAAACCAATGGAGATTTAATGGCTGCTACTGCCACGAGTAACCTGTACCGCAATCCGAGATTTAAGAGCACAATGAAAGCAATCATGTCCACGACCTGCAGCCCAGATGAAGTCATGTTTGGTTCTGATTTCCATCAATCACTGTATTGTCATCTGCTGTGTGGGCTTATTTTTAGGGATGAAATTCAGATGATATTCTCGACGTTTGCTCATAGCATCGTTCATGCGTTTAGAACGTTTGAAAACATCTGGGAAGAGCTATGTTGTGACATTCAAAGTGGTGTTCTCTCCAGCCGAATCACGTCACCTTCCATTCGTGCAGGGATGTCGAAATTGCTGAAACCGAATCCCGAACTGGCGAATTTGGTGTACGAAAAGTGTGTTGGATTGAGTAATTGGTATGGATTGATACCCCAACTCTTCCCTAACGCAAAGTACATATATGGAATCATGACTGGATCGATGGAACCATATGTGAAAAAGTTGAGACACTATGCAGGAGAGCTGCCTTTGATGAGTGCTGATTATGGTTCATCCGAAGGATGGATTGGTGCAAATACAAACCCGAAAATGTCTCCCGAATTCGCTACTTTCACTGTGCTTCCCAATATTGGTTACTTTGAATTCATTCCTCTCGGGGACGACATCTTCGAGCACGATAAGACTCAATCTTCTTCAATCTGCAACGAGTTGAAACCGGTGGATCTTACTCAAGTCCAAGTTGGTAAAGAGTATGAGATTCTAATCACAAGTGTTGCAGGTCTGTATAGGTATAGGCTTGGAGATGTGGTTAAGGTCACAGGCTTCCATAACTCGACCCCGGTACTCAAATTCGTCTGTCGGAGAAACCTCCTGCTAACGATCAACATCGACAAGAACACGGAGAAAGACTTGCAGATAGCTGTTGAGGAAGCCTCAAAGCTACTAGCAGAGGAGAAACTCGAAGTTGTCGATTTCACGAGCTACGTGGATCGATCTACAGAGCCTGGCCACTACGTCATCTTCTGGGAGATGAGTGGTGAAGCTTATAACGACGACATTTTGAAGCAATGCTGCAACTGTTTGGATAGTTCCTTTGTTGATGCAGGCTATGTTAGCTCACGTAGAGTCAAGGCAATTGGCGCGTTGGAACTTCGGGTGGTCCAGAGAGGAACATTCCAGAAGATTCTGGATCACTTCCTGTCGTTAGGGGCGGCGGTGAGTCAGTTTAAAACGCCTCGATGCATCGGTCCAGCCAACAATAAGGTGCTGCAGATCTTGTGCAGTAATGTCAACAAGGTCTACTTCAGTACTGCTTTTCAGTAA

Protein sequence

MLEKMEEFDMEKVIEEFEELTKDAERVQRETLKMILEENGSAEYLQNLGLNGKTDPVSFKACVPIVTHEDLEPYIQRIADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELIETTIQIFRTSFAFRNRDFPIEKGRSLMFIYGSKQFKTNGDLMAATATSNLYRNPRFKSTMKAIMSTTCSPDEVMFGSDFHQSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFENIWEELCCDIQSGVLSSRITSPSIRAGMSKLLKPNPELANLVYEKCVGLSNWYGLIPQLFPNAKYIYGIMTGSMEPYVKKLRHYAGELPLMSADYGSSEGWIGANTNPKMSPEFATFTVLPNIGYFEFIPLGDDIFEHDKTQSSSICNELKPVDLTQVQVGKEYEILITSVAGLYRYRLGDVVKVTGFHNSTPVLKFVCRRNLLLTINIDKNTEKDLQIAVEEASKLLAEEKLEVVDFTSYVDRSTEPGHYVIFWEMSGEAYNDDILKQCCNCLDSSFVDAGYVSSRRVKAIGALELRVVQRGTFQKILDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVNKVYFSTAFQ
Homology
BLAST of Spg013797 vs. NCBI nr
Match: XP_022158613.1 (jasmonic acid-amido synthetase JAR1-like isoform X1 [Momordica charantia])

HSP 1 Score: 1059.7 bits (2739), Expect = 9.3e-306
Identity = 522/585 (89.23%), Postives = 557/585 (95.21%), Query Frame = 0

Query: 1   MLEKMEEFDMEKVIEEFEELTKDAERVQRETLKMILEENGSAEYLQNLGLNGKTDPVSFK 60
           MLEKMEEFD EKVIEE E ++K+A RVQRETLK ILEENGS+EYLQNLGLNG TDP SFK
Sbjct: 1   MLEKMEEFDTEKVIEEVEAMSKEAGRVQRETLKRILEENGSSEYLQNLGLNGNTDPESFK 60

Query: 61  ACVPIVTHEDLEPYIQRIADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELIETTI 120
           ACVPIVTHEDLEPYIQRIAD   A ILT KPITTISLSSGTT+GKPKFVPFNDEL+++TI
Sbjct: 61  ACVPIVTHEDLEPYIQRIADSHHASILTAKPITTISLSSGTTEGKPKFVPFNDELVDSTI 120

Query: 121 QIFRTSFAFRNRDFPIEKGRSLMFIYGSKQFKTNGDLMAATATSNLYRNPRFKSTMKAIM 180
           QIF+ SFAFRNRDFPI KGRSLMFIYGSKQFKTNG L AATATSNLYR+P+FKSTMK+IM
Sbjct: 121 QIFQASFAFRNRDFPIGKGRSLMFIYGSKQFKTNGGLTAATATSNLYRDPKFKSTMKSIM 180

Query: 181 STTCSPDEVMFGSDFHQSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFENIWEELCC 240
           S +CSPDEV++GSDFHQSLYCHLLCGLIFRDEIQ+IFSTFAHSIVHAFRTFE+IWEELCC
Sbjct: 181 SMSCSPDEVIYGSDFHQSLYCHLLCGLIFRDEIQLIFSTFAHSIVHAFRTFEHIWEELCC 240

Query: 241 DIQSGVLSSRITSPSIRAGMSKLLKPNPELANLVYEKCVGLSNWYGLIPQLFPNAKYIYG 300
           DI++G+L+SRITSPSIRAGMS LLKPNPELA+LVYEKCVGLS WYGLIPQLFPNAKYIYG
Sbjct: 241 DIRNGILTSRITSPSIRAGMSNLLKPNPELADLVYEKCVGLSTWYGLIPQLFPNAKYIYG 300

Query: 301 IMTGSMEPYVKKLRHYAGELPLMSADYGSSEGWIGANTNPKMSPEFATFTVLPNIGYFEF 360
           IMTGSMEPYVKKLRHYAG+LPLMSADYGSSEGWIGAN NPK+SPEFATFTVLPNIGYFEF
Sbjct: 301 IMTGSMEPYVKKLRHYAGDLPLMSADYGSSEGWIGANINPKLSPEFATFTVLPNIGYFEF 360

Query: 361 IPLGDDIFEHDKTQSSSICNELKPVDLTQVQVGKEYEILITSVAGLYRYRLGDVVKVTGF 420
           IPLG +I E+DK+QSS IC+ELKPVDLTQVQVGKEYEILITSVAG+YRYRLGDVVKVTGF
Sbjct: 361 IPLG-EISENDKSQSSLICSELKPVDLTQVQVGKEYEILITSVAGMYRYRLGDVVKVTGF 420

Query: 421 HNSTPVLKFVCRRNLLLTINIDKNTEKDLQIAVEEASKLLAEEKLEVVDFTSYVDRSTEP 480
           +NSTP LKFVCRRNLLLTINIDKNTEKDLQIAV EASKLLAEEKLEVVDFTSYVDRST+P
Sbjct: 421 YNSTPELKFVCRRNLLLTINIDKNTEKDLQIAVGEASKLLAEEKLEVVDFTSYVDRSTDP 480

Query: 481 GHYVIFWEMSGEAYNDDILKQCCNCLDSSFVDAGYVSSRRVKAIGALELRVVQRGTFQKI 540
           GHYVIFWEMSGEAYNDD+L +CCN LD+SFVDAGYVSSRRVK+IGALELRVVQRGTFQKI
Sbjct: 481 GHYVIFWEMSGEAYNDDLLNRCCNRLDTSFVDAGYVSSRRVKSIGALELRVVQRGTFQKI 540

Query: 541 LDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVNKVYFSTAFQ 586
           LDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNV KVYFSTAFQ
Sbjct: 541 LDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVIKVYFSTAFQ 584

BLAST of Spg013797 vs. NCBI nr
Match: XP_022158615.1 (jasmonic acid-amido synthetase JAR1-like isoform X2 [Momordica charantia])

HSP 1 Score: 982.2 bits (2538), Expect = 1.9e-282
Identity = 494/585 (84.44%), Postives = 524/585 (89.57%), Query Frame = 0

Query: 1   MLEKMEEFDMEKVIEEFEELTKDAERVQRETLKMILEENGSAEYLQNLGLNGKTDPVSFK 60
           MLEKMEEFD EKVIEE E ++K+A RVQRETLK ILEENGS+EYLQNLGLNG TDP SFK
Sbjct: 1   MLEKMEEFDTEKVIEEVEAMSKEAGRVQRETLKRILEENGSSEYLQNLGLNGNTDPESFK 60

Query: 61  ACVPIVTHEDLEPYIQRIADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELIETTI 120
           ACVPIVTHEDLEPYIQRIAD   A ILT KPITTISL                       
Sbjct: 61  ACVPIVTHEDLEPYIQRIADSHHASILTAKPITTISL----------------------- 120

Query: 121 QIFRTSFAFRNRDFPIEKGRSLMFIYGSKQFKTNGDLMAATATSNLYRNPRFKSTMKAIM 180
                      RDFPI KGRSLMFIYGSKQFKTNG L AATATSNLYR+P+FKSTMK+IM
Sbjct: 121 -----------RDFPIGKGRSLMFIYGSKQFKTNGGLTAATATSNLYRDPKFKSTMKSIM 180

Query: 181 STTCSPDEVMFGSDFHQSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFENIWEELCC 240
           S +CSPDEV++GSDFHQSLYCHLLCGLIFRDEIQ+IFSTFAHSIVHAFRTFE+IWEELCC
Sbjct: 181 SMSCSPDEVIYGSDFHQSLYCHLLCGLIFRDEIQLIFSTFAHSIVHAFRTFEHIWEELCC 240

Query: 241 DIQSGVLSSRITSPSIRAGMSKLLKPNPELANLVYEKCVGLSNWYGLIPQLFPNAKYIYG 300
           DI++G+L+SRITSPSIRAGMS LLKPNPELA+LVYEKCVGLS WYGLIPQLFPNAKYIYG
Sbjct: 241 DIRNGILTSRITSPSIRAGMSNLLKPNPELADLVYEKCVGLSTWYGLIPQLFPNAKYIYG 300

Query: 301 IMTGSMEPYVKKLRHYAGELPLMSADYGSSEGWIGANTNPKMSPEFATFTVLPNIGYFEF 360
           IMTGSMEPYVKKLRHYAG+LPLMSADYGSSEGWIGAN NPK+SPEFATFTVLPNIGYFEF
Sbjct: 301 IMTGSMEPYVKKLRHYAGDLPLMSADYGSSEGWIGANINPKLSPEFATFTVLPNIGYFEF 360

Query: 361 IPLGDDIFEHDKTQSSSICNELKPVDLTQVQVGKEYEILITSVAGLYRYRLGDVVKVTGF 420
           IPLG +I E+DK+QSS IC+ELKPVDLTQVQVGKEYEILITSVAG+YRYRLGDVVKVTGF
Sbjct: 361 IPLG-EISENDKSQSSLICSELKPVDLTQVQVGKEYEILITSVAGMYRYRLGDVVKVTGF 420

Query: 421 HNSTPVLKFVCRRNLLLTINIDKNTEKDLQIAVEEASKLLAEEKLEVVDFTSYVDRSTEP 480
           +NSTP LKFVCRRNLLLTINIDKNTEKDLQIAV EASKLLAEEKLEVVDFTSYVDRST+P
Sbjct: 421 YNSTPELKFVCRRNLLLTINIDKNTEKDLQIAVGEASKLLAEEKLEVVDFTSYVDRSTDP 480

Query: 481 GHYVIFWEMSGEAYNDDILKQCCNCLDSSFVDAGYVSSRRVKAIGALELRVVQRGTFQKI 540
           GHYVIFWEMSGEAYNDD+L +CCN LD+SFVDAGYVSSRRVK+IGALELRVVQRGTFQKI
Sbjct: 481 GHYVIFWEMSGEAYNDDLLNRCCNRLDTSFVDAGYVSSRRVKSIGALELRVVQRGTFQKI 540

Query: 541 LDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVNKVYFSTAFQ 586
           LDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNV KVYFSTAFQ
Sbjct: 541 LDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVIKVYFSTAFQ 550

BLAST of Spg013797 vs. NCBI nr
Match: XP_041001957.1 (jasmonoyl--L-amino acid synthetase JAR4-like isoform X3 [Juglans microcarpa x Juglans regia] >XP_041001958.1 jasmonoyl--L-amino acid synthetase JAR4-like isoform X3 [Juglans microcarpa x Juglans regia])

HSP 1 Score: 949.9 bits (2454), Expect = 1.0e-272
Identity = 458/586 (78.16%), Postives = 523/586 (89.25%), Query Frame = 0

Query: 1   MLEKMEEFDMEKVIEEFEELTKDAERVQRETLKMILEENGSAEYLQNLGLNGKTDPVSFK 60
           MLEKMEEFD EKVIEEFE +T+DA +VQRETLK ILEENGSAEYLQ+LGLNG+TDP SFK
Sbjct: 1   MLEKMEEFDPEKVIEEFEAITQDAGKVQRETLKKILEENGSAEYLQSLGLNGRTDPESFK 60

Query: 61  ACVPIVTHEDLEPYIQRIADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELIETTI 120
            CVP+VTHE+LEPYI RIADG  +PILTGKPITTISLSSGTTQGKPKFVPFNDEL+E+T+
Sbjct: 61  TCVPLVTHEELEPYIHRIADGDPSPILTGKPITTISLSSGTTQGKPKFVPFNDELMESTM 120

Query: 121 QIFRTSFAFRNRDFPIEKGRSLMFIYGSKQFKTNGDLMAATATSNLYRNPRFKSTMKAIM 180
           QI+RTSFAFRNR+FPI  G++L FIY SKQF+T G L A TAT+N+YRN +FK TMKAI 
Sbjct: 121 QIYRTSFAFRNREFPIGNGKALQFIYSSKQFQTKGGLAAGTATTNVYRNSQFKETMKAIQ 180

Query: 181 STTCSPDEVMFGSDFHQSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFENIWEELCC 240
           S +C PDEV+FG DFHQSLYCHLLCGLIFRDE+Q +FSTFAHSI+HAFRTFE +WEELC 
Sbjct: 181 SQSCCPDEVIFGPDFHQSLYCHLLCGLIFRDEVQFVFSTFAHSIIHAFRTFEQVWEELCI 240

Query: 241 DIQSGVLSSRITSPSIRAGMSKLLKPNPELANLVYEKCVGLSNWYGLIPQLFPNAKYIYG 300
           DI+ GVLSSR+++PSIRA MSKLLKPNPELA+LVY+K  GLSNWY LIP+LFPNAKYIYG
Sbjct: 241 DIREGVLSSRVSAPSIRAAMSKLLKPNPELADLVYKKITGLSNWYRLIPELFPNAKYIYG 300

Query: 301 IMTGSMEPYVKKLRHYAGELPLMSADYGSSEGWIGANTNPKMSPEFATFTVLPNIGYFEF 360
           IMTGSMEPY++KLRHYAGELPL+SADYGSSEGWIGAN NPK+ PE ATF VLPNIG+FEF
Sbjct: 301 IMTGSMEPYLRKLRHYAGELPLLSADYGSSEGWIGANVNPKLPPELATFAVLPNIGFFEF 360

Query: 361 IPLGDDIFEHDKTQ--SSSICNELKPVDLTQVQVGKEYEILITSVAGLYRYRLGDVVKVT 420
           IPL  +I + D+ Q   S    E +PVDLT+V++G++YEI++T+ AGLYRYRLGDVVKV 
Sbjct: 361 IPLKHNIEDQDQNQMDPSFFQIEPEPVDLTEVRIGEDYEIIVTNFAGLYRYRLGDVVKVM 420

Query: 421 GFHNSTPVLKFVCRRNLLLTINIDKNTEKDLQIAVEEASKLLAEEKLEVVDFTSYVDRST 480
           GFHNSTP LKFVCRRNLLLTINIDKNTEKDLQ+AVEEASKLL EEKLEVVD++S+VD ST
Sbjct: 421 GFHNSTPELKFVCRRNLLLTINIDKNTEKDLQLAVEEASKLLGEEKLEVVDYSSHVDSST 480

Query: 481 EPGHYVIFWEMSGEAYNDDILKQCCNCLDSSFVDAGYVSSRRVKAIGALELRVVQRGTFQ 540
           +PGHYVIFWE+SGEA  +++LK+CCNCLD SFVDAGYVSSR+V AIGALELRVV+RGTFQ
Sbjct: 481 DPGHYVIFWEISGEA-GEEVLKECCNCLDRSFVDAGYVSSRKVNAIGALELRVVRRGTFQ 540

Query: 541 KILDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVNKVYFSTAF 585
           KILDH+L LG+AVSQFKTPRC+GP NN VLQILC NV + YFSTAF
Sbjct: 541 KILDHYLGLGSAVSQFKTPRCVGPTNNLVLQILCDNVVESYFSTAF 585

BLAST of Spg013797 vs. NCBI nr
Match: XP_041001955.1 (jasmonoyl--L-amino acid synthetase JAR4-like isoform X2 [Juglans microcarpa x Juglans regia] >XP_041001956.1 jasmonoyl--L-amino acid synthetase JAR4-like isoform X2 [Juglans microcarpa x Juglans regia])

HSP 1 Score: 949.9 bits (2454), Expect = 1.0e-272
Identity = 458/586 (78.16%), Postives = 523/586 (89.25%), Query Frame = 0

Query: 1   MLEKMEEFDMEKVIEEFEELTKDAERVQRETLKMILEENGSAEYLQNLGLNGKTDPVSFK 60
           MLEKMEEFD EKVIEEFE +T+DA +VQRETLK ILEENGSAEYLQ+LGLNG+TDP SFK
Sbjct: 22  MLEKMEEFDPEKVIEEFEAITQDAGKVQRETLKKILEENGSAEYLQSLGLNGRTDPESFK 81

Query: 61  ACVPIVTHEDLEPYIQRIADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELIETTI 120
            CVP+VTHE+LEPYI RIADG  +PILTGKPITTISLSSGTTQGKPKFVPFNDEL+E+T+
Sbjct: 82  TCVPLVTHEELEPYIHRIADGDPSPILTGKPITTISLSSGTTQGKPKFVPFNDELMESTM 141

Query: 121 QIFRTSFAFRNRDFPIEKGRSLMFIYGSKQFKTNGDLMAATATSNLYRNPRFKSTMKAIM 180
           QI+RTSFAFRNR+FPI  G++L FIY SKQF+T G L A TAT+N+YRN +FK TMKAI 
Sbjct: 142 QIYRTSFAFRNREFPIGNGKALQFIYSSKQFQTKGGLAAGTATTNVYRNSQFKETMKAIQ 201

Query: 181 STTCSPDEVMFGSDFHQSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFENIWEELCC 240
           S +C PDEV+FG DFHQSLYCHLLCGLIFRDE+Q +FSTFAHSI+HAFRTFE +WEELC 
Sbjct: 202 SQSCCPDEVIFGPDFHQSLYCHLLCGLIFRDEVQFVFSTFAHSIIHAFRTFEQVWEELCI 261

Query: 241 DIQSGVLSSRITSPSIRAGMSKLLKPNPELANLVYEKCVGLSNWYGLIPQLFPNAKYIYG 300
           DI+ GVLSSR+++PSIRA MSKLLKPNPELA+LVY+K  GLSNWY LIP+LFPNAKYIYG
Sbjct: 262 DIREGVLSSRVSAPSIRAAMSKLLKPNPELADLVYKKITGLSNWYRLIPELFPNAKYIYG 321

Query: 301 IMTGSMEPYVKKLRHYAGELPLMSADYGSSEGWIGANTNPKMSPEFATFTVLPNIGYFEF 360
           IMTGSMEPY++KLRHYAGELPL+SADYGSSEGWIGAN NPK+ PE ATF VLPNIG+FEF
Sbjct: 322 IMTGSMEPYLRKLRHYAGELPLLSADYGSSEGWIGANVNPKLPPELATFAVLPNIGFFEF 381

Query: 361 IPLGDDIFEHDKTQ--SSSICNELKPVDLTQVQVGKEYEILITSVAGLYRYRLGDVVKVT 420
           IPL  +I + D+ Q   S    E +PVDLT+V++G++YEI++T+ AGLYRYRLGDVVKV 
Sbjct: 382 IPLKHNIEDQDQNQMDPSFFQIEPEPVDLTEVRIGEDYEIIVTNFAGLYRYRLGDVVKVM 441

Query: 421 GFHNSTPVLKFVCRRNLLLTINIDKNTEKDLQIAVEEASKLLAEEKLEVVDFTSYVDRST 480
           GFHNSTP LKFVCRRNLLLTINIDKNTEKDLQ+AVEEASKLL EEKLEVVD++S+VD ST
Sbjct: 442 GFHNSTPELKFVCRRNLLLTINIDKNTEKDLQLAVEEASKLLGEEKLEVVDYSSHVDSST 501

Query: 481 EPGHYVIFWEMSGEAYNDDILKQCCNCLDSSFVDAGYVSSRRVKAIGALELRVVQRGTFQ 540
           +PGHYVIFWE+SGEA  +++LK+CCNCLD SFVDAGYVSSR+V AIGALELRVV+RGTFQ
Sbjct: 502 DPGHYVIFWEISGEA-GEEVLKECCNCLDRSFVDAGYVSSRKVNAIGALELRVVRRGTFQ 561

Query: 541 KILDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVNKVYFSTAF 585
           KILDH+L LG+AVSQFKTPRC+GP NN VLQILC NV + YFSTAF
Sbjct: 562 KILDHYLGLGSAVSQFKTPRCVGPTNNLVLQILCDNVVESYFSTAF 606

BLAST of Spg013797 vs. NCBI nr
Match: XP_041001954.1 (jasmonoyl--L-amino acid synthetase JAR4-like isoform X1 [Juglans microcarpa x Juglans regia])

HSP 1 Score: 949.9 bits (2454), Expect = 1.0e-272
Identity = 458/586 (78.16%), Postives = 523/586 (89.25%), Query Frame = 0

Query: 1   MLEKMEEFDMEKVIEEFEELTKDAERVQRETLKMILEENGSAEYLQNLGLNGKTDPVSFK 60
           MLEKMEEFD EKVIEEFE +T+DA +VQRETLK ILEENGSAEYLQ+LGLNG+TDP SFK
Sbjct: 46  MLEKMEEFDPEKVIEEFEAITQDAGKVQRETLKKILEENGSAEYLQSLGLNGRTDPESFK 105

Query: 61  ACVPIVTHEDLEPYIQRIADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELIETTI 120
            CVP+VTHE+LEPYI RIADG  +PILTGKPITTISLSSGTTQGKPKFVPFNDEL+E+T+
Sbjct: 106 TCVPLVTHEELEPYIHRIADGDPSPILTGKPITTISLSSGTTQGKPKFVPFNDELMESTM 165

Query: 121 QIFRTSFAFRNRDFPIEKGRSLMFIYGSKQFKTNGDLMAATATSNLYRNPRFKSTMKAIM 180
           QI+RTSFAFRNR+FPI  G++L FIY SKQF+T G L A TAT+N+YRN +FK TMKAI 
Sbjct: 166 QIYRTSFAFRNREFPIGNGKALQFIYSSKQFQTKGGLAAGTATTNVYRNSQFKETMKAIQ 225

Query: 181 STTCSPDEVMFGSDFHQSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFENIWEELCC 240
           S +C PDEV+FG DFHQSLYCHLLCGLIFRDE+Q +FSTFAHSI+HAFRTFE +WEELC 
Sbjct: 226 SQSCCPDEVIFGPDFHQSLYCHLLCGLIFRDEVQFVFSTFAHSIIHAFRTFEQVWEELCI 285

Query: 241 DIQSGVLSSRITSPSIRAGMSKLLKPNPELANLVYEKCVGLSNWYGLIPQLFPNAKYIYG 300
           DI+ GVLSSR+++PSIRA MSKLLKPNPELA+LVY+K  GLSNWY LIP+LFPNAKYIYG
Sbjct: 286 DIREGVLSSRVSAPSIRAAMSKLLKPNPELADLVYKKITGLSNWYRLIPELFPNAKYIYG 345

Query: 301 IMTGSMEPYVKKLRHYAGELPLMSADYGSSEGWIGANTNPKMSPEFATFTVLPNIGYFEF 360
           IMTGSMEPY++KLRHYAGELPL+SADYGSSEGWIGAN NPK+ PE ATF VLPNIG+FEF
Sbjct: 346 IMTGSMEPYLRKLRHYAGELPLLSADYGSSEGWIGANVNPKLPPELATFAVLPNIGFFEF 405

Query: 361 IPLGDDIFEHDKTQ--SSSICNELKPVDLTQVQVGKEYEILITSVAGLYRYRLGDVVKVT 420
           IPL  +I + D+ Q   S    E +PVDLT+V++G++YEI++T+ AGLYRYRLGDVVKV 
Sbjct: 406 IPLKHNIEDQDQNQMDPSFFQIEPEPVDLTEVRIGEDYEIIVTNFAGLYRYRLGDVVKVM 465

Query: 421 GFHNSTPVLKFVCRRNLLLTINIDKNTEKDLQIAVEEASKLLAEEKLEVVDFTSYVDRST 480
           GFHNSTP LKFVCRRNLLLTINIDKNTEKDLQ+AVEEASKLL EEKLEVVD++S+VD ST
Sbjct: 466 GFHNSTPELKFVCRRNLLLTINIDKNTEKDLQLAVEEASKLLGEEKLEVVDYSSHVDSST 525

Query: 481 EPGHYVIFWEMSGEAYNDDILKQCCNCLDSSFVDAGYVSSRRVKAIGALELRVVQRGTFQ 540
           +PGHYVIFWE+SGEA  +++LK+CCNCLD SFVDAGYVSSR+V AIGALELRVV+RGTFQ
Sbjct: 526 DPGHYVIFWEISGEA-GEEVLKECCNCLDRSFVDAGYVSSRKVNAIGALELRVVRRGTFQ 585

Query: 541 KILDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVNKVYFSTAF 585
           KILDH+L LG+AVSQFKTPRC+GP NN VLQILC NV + YFSTAF
Sbjct: 586 KILDHYLGLGSAVSQFKTPRCVGPTNNLVLQILCDNVVESYFSTAF 630

BLAST of Spg013797 vs. ExPASy Swiss-Prot
Match: A0A314KSQ4 (Jasmonoyl--L-amino acid synthetase JAR6 OS=Nicotiana attenuata OX=49451 GN=JAR6 PE=1 SV=2)

HSP 1 Score: 877.9 bits (2267), Expect = 6.5e-254
Identity = 423/585 (72.31%), Postives = 494/585 (84.44%), Query Frame = 0

Query: 1   MLEKME-EFDMEKVIEEFEELTKDAERVQRETLKMILEENGSAEYLQNLGLNGKTDPVSF 60
           ++EK+E +FD EKVIEEFE+LTKDA ++Q ETLK ILE+NG  EYLQ  GLNG+TDP +F
Sbjct: 4   LVEKIEKKFDQEKVIEEFEDLTKDAGKIQEETLKKILEQNGGTEYLQLWGLNGRTDPQTF 63

Query: 61  KACVPIVTHEDLEPYIQRIADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELIETT 120
           K CVPIVTH DLEPYIQRIADG  +PILTGKPI TISLSSGTTQGKPKFVPFNDEL+E+T
Sbjct: 64  KNCVPIVTHNDLEPYIQRIADGDLSPILTGKPIETISLSSGTTQGKPKFVPFNDELMEST 123

Query: 121 IQIFRTSFAFRNRDFPIEKGRSLMFIYGSKQFKTNGDLMAATATSNLYRNPRFKSTMKAI 180
           +QIF+TSFAFRNR+FPI  G++L FIY SKQFKT G L A TAT+N+YRN +FK TMKA+
Sbjct: 124 MQIFKTSFAFRNREFPIGNGKALQFIYSSKQFKTKGGLAAGTATTNVYRNAQFKKTMKAM 183

Query: 181 MSTTCSPDEVMFGSDFHQSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFENIWEELC 240
            +  CSPDEV+FG DFHQSLYCHLLCGLIF DE+Q++ STFAHSIVHAFRTFE +WE L 
Sbjct: 184 CTPCCSPDEVIFGPDFHQSLYCHLLCGLIFHDEVQVVSSTFAHSIVHAFRTFEQVWEALV 243

Query: 241 CDIQSGVLSSRITSPSIRAGMSKLLKPNPELANLVYEKCVGLSNWYGLIPQLFPNAKYIY 300
            DI+ GVLSSR+T PSIR  MSKLLKP+PELA+ +Y KC  LSNWYGLIP LFPN +YIY
Sbjct: 244 VDIREGVLSSRVTVPSIRLAMSKLLKPDPELADTIYNKCSRLSNWYGLIPDLFPNTRYIY 303

Query: 301 GIMTGSMEPYVKKLRHYAGELPLMSADYGSSEGWIGANTNPKMSPEFATFTVLPNIGYFE 360
           GIMTGSMEPY+KKLRHYAGELPL+SADYGSSEGW+G N NPK+ PE  T+ VLPNIGYFE
Sbjct: 304 GIMTGSMEPYLKKLRHYAGELPLLSADYGSSEGWVGVNVNPKLPPELVTYAVLPNIGYFE 363

Query: 361 FIPLGDDIFEHDKTQSSSICNELKPVDLTQVQVGKEYEILITSVAGLYRYRLGDVVKVTG 420
           FIPLG ++   ++  S        PV LT+V++G+EYE++ T+ AGLYRYRLGDVVKV G
Sbjct: 364 FIPLGGNLNGIEQANS--------PVGLTEVKLGEEYEVVFTNFAGLYRYRLGDVVKVKG 423

Query: 421 FHNSTPVLKFVCRRNLLLTINIDKNTEKDLQIAVEEASKLLAEEKLEVVDFTSYVDRSTE 480
           FHN TP L+FVCR NLLL+INIDKNTEKDLQ+AVE A+K L +EKLEVVDFTS+V+ S +
Sbjct: 424 FHNGTPELQFVCRSNLLLSINIDKNTEKDLQLAVEAAAKRLVDEKLEVVDFTSHVNVSAD 483

Query: 481 PGHYVIFWEMSGEAYNDDILKQCCNCLDSSFVDAGYVSSRRVKAIGALELRVVQRGTFQK 540
           PGHYVIFWE+SGEA  D++L+ CCNCLD SF+DAGYVSSR+V AIGALELR+V+RGTF K
Sbjct: 484 PGHYVIFWELSGEA-TDEMLQDCCNCLDRSFIDAGYVSSRKVNAIGALELRIVKRGTFHK 543

Query: 541 ILDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVNKVYFSTAF 585
           ILDHF+ LG AVSQFKTPRC+GP N+ +LQIL SNV + Y STAF
Sbjct: 544 ILDHFVGLGGAVSQFKTPRCVGPKNSSLLQILSSNVVETYVSTAF 579

BLAST of Spg013797 vs. ExPASy Swiss-Prot
Match: A0A1J6KGJ9 (Jasmonoyl--L-amino acid synthetase JAR4 OS=Nicotiana attenuata OX=49451 GN=JAR4 PE=1 SV=1)

HSP 1 Score: 874.8 bits (2259), Expect = 5.5e-253
Identity = 419/584 (71.75%), Postives = 499/584 (85.45%), Query Frame = 0

Query: 1   MLEKMEEFDMEKVIEEFEELTKDAERVQRETLKMILEENGSAEYLQNLGLNGKTDPVSFK 60
           ++EK E+FD E+VIEEFE LTKDA ++Q ETL+ ILEENG  EYLQ  GLNGKTD +SFK
Sbjct: 4   VVEKTEKFDPEEVIEEFEVLTKDAGKIQEETLQKILEENGGTEYLQQWGLNGKTDSLSFK 63

Query: 61  ACVPIVTHEDLEPYIQRIADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELIETTI 120
            C+PIVTH+DLEPYI RIADG  +PILTGKPITTISLSSGTTQGKPKFVPFN+EL+E+T+
Sbjct: 64  NCIPIVTHKDLEPYIHRIADGDLSPILTGKPITTISLSSGTTQGKPKFVPFNEELMESTM 123

Query: 121 QIFRTSFAFRNRDFPIEKGRSLMFIYGSKQFKTNGDLMAATATSNLYRNPRFKSTMKAIM 180
           QIF+TSF FRNR+FP+  G++L FIYGSKQFKT G L A TAT+N+YRN +FK TMKA+ 
Sbjct: 124 QIFKTSFVFRNREFPVVNGKALQFIYGSKQFKTKGGLAAGTATTNVYRNAQFKKTMKAMQ 183

Query: 181 STTCSPDEVMFGSDFHQSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFENIWEELCC 240
           +  CSPDEV+FG DF QSLYCHLLCGLIFRDE+Q++ STFAHSIVHAFR FE IW+EL  
Sbjct: 184 TPCCSPDEVIFGPDFQQSLYCHLLCGLIFRDEVQVVSSTFAHSIVHAFRNFEQIWQELVT 243

Query: 241 DIQSGVLSSRITSPSIRAGMSKLLKPNPELANLVYEKCVGLSNWYGLIPQLFPNAKYIYG 300
           +I+ GVLSSR+  PS+RA MSKLLKP+PELA+ ++ KC  LSNWYGLIP+LFPN +YIYG
Sbjct: 244 NIREGVLSSRVIVPSMRAAMSKLLKPDPELADTIFNKCSRLSNWYGLIPELFPNTRYIYG 303

Query: 301 IMTGSMEPYVKKLRHYAGELPLMSADYGSSEGWIGANTNPKMSPEFATFTVLPNIGYFEF 360
           IMTGSMEPY+KKLRHYAG+LPL+SADYGSSEGWIGAN NP++ PE  T+ VLPNIGYFEF
Sbjct: 304 IMTGSMEPYLKKLRHYAGDLPLLSADYGSSEGWIGANVNPELPPELVTYAVLPNIGYFEF 363

Query: 361 IPLGDDIFEHDKTQSSSICNELKPVDLTQVQVGKEYEILITSVAGLYRYRLGDVVKVTGF 420
           IPL +++             E  PV LT+V++G+EYEI++T+ AGLYRYRLGDVVK+ GF
Sbjct: 364 IPLMENLDG----------LEPMPVGLTEVKLGEEYEIVVTNFAGLYRYRLGDVVKIKGF 423

Query: 421 HNSTPVLKFVCRRNLLLTINIDKNTEKDLQIAVEEASKLLAEEKLEVVDFTSYVDRSTEP 480
           HN TP L+F+CRRNLLL+INIDKNTEKDLQ+AVE A+K+L++EKLEVVDFTS+V+ S +P
Sbjct: 424 HNGTPELQFICRRNLLLSINIDKNTEKDLQLAVEAAAKILSDEKLEVVDFTSHVNVSADP 483

Query: 481 GHYVIFWEMSGEAYNDDILKQCCNCLDSSFVDAGYVSSRRVKAIGALELRVVQRGTFQKI 540
           GHYVIFWE++GEA +++ILK+CCNCLD SFVDAGYV SR+V AIGALELR+V+RGTF KI
Sbjct: 484 GHYVIFWELNGEA-SEEILKECCNCLDKSFVDAGYVGSRKVHAIGALELRIVKRGTFHKI 543

Query: 541 LDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVNKVYFSTAF 585
           LDHF+ LGAAVSQFKTPRC+GP N  VLQIL SNV + YFSTAF
Sbjct: 544 LDHFVGLGAAVSQFKTPRCVGPTNLSVLQILSSNVVESYFSTAF 576

BLAST of Spg013797 vs. ExPASy Swiss-Prot
Match: Q6I581 (Jasmonoyl--L-amino acid synthetase GH3.5 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 824.7 bits (2129), Expect = 6.6e-238
Identity = 379/574 (66.03%), Postives = 479/574 (83.45%), Query Frame = 0

Query: 11  EKVIEEFEELTKDAERVQRETLKMILEENGSAEYLQNLGLNGKTDPVSFKACVPIVTHED 70
           E+ I EFE LT+DA RVQ++TLK ILE N SAEYLQN GL G+TD  S+K+C+P+  H D
Sbjct: 7   EETINEFEMLTRDAARVQKDTLKKILEINASAEYLQNFGLGGRTDAESYKSCIPLCVHND 66

Query: 71  LEPYIQRIADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELIETTIQIFRTSFAFR 130
           +EPYIQRI DG  +P++TG+PIT +SLSSGTT GKPKF+PFNDEL+ETT+QI+RTS+AFR
Sbjct: 67  IEPYIQRIVDGDTSPVVTGEPITNLSLSSGTTHGKPKFIPFNDELLETTLQIYRTSYAFR 126

Query: 131 NRDFPIEKGRSLMFIYGSKQFKTNGDLMAATATSNLYRNPRFKSTMKAIMSTTCSPDEVM 190
           NR++PI +G++L F+YGSKQ  T G ++A TAT+NLYR  R+K  MK I S  CSPDEV+
Sbjct: 127 NREYPIGQGKALQFVYGSKQVITKGGILATTATTNLYRRQRYKEGMKDIQSQCCSPDEVI 186

Query: 191 FGSDFHQSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFENIWEELCCDIQSGVLSSR 250
           FG DFHQSLYCHLLCGLI+ +E+  +FSTFAHS+VHAF+TFE +WE+LC DI+ GVLS +
Sbjct: 187 FGPDFHQSLYCHLLCGLIYSEEVHSVFSTFAHSLVHAFQTFEEVWEDLCTDIRDGVLSKK 246

Query: 251 ITSPSIRAGMSKLLKPNPELANLVYEKCVGLSNWYGLIPQLFPNAKYIYGIMTGSMEPYV 310
           +T+PSIR  +SK+LKPNPELA+ +Y+KC+GLSNWYG+IP L+PNAKY+YGIMTGSMEPY+
Sbjct: 247 VTAPSIREAVSKILKPNPELADSIYKKCIGLSNWYGVIPALWPNAKYVYGIMTGSMEPYL 306

Query: 311 KKLRHYAGELPLMSADYGSSEGWIGANTNPKMSPEFATFTVLPNIGYFEFIPLGDDIFEH 370
           KKLRHYAG LPL+SADYG+SEGW+G+N +P + PE  T+ VLP +GYFEFIPL   I E 
Sbjct: 307 KKLRHYAGNLPLISADYGASEGWVGSNIDPTVPPEQVTYAVLPQVGYFEFIPLEKPIGEE 366

Query: 371 DKTQSSSICNELKPVDLTQVQVGKEYEILITSVAGLYRYRLGDVVKVTGFHNSTPVLKFV 430
            +  +S    E  PV LT+V+VGK YE++IT+ AGLYRYRLGDVVK+  FHNSTP L+F+
Sbjct: 367 TENSASIHYIESDPVGLTEVEVGKIYEVVITNFAGLYRYRLGDVVKIARFHNSTPELQFI 426

Query: 431 CRRNLLLTINIDKNTEKDLQIAVEEASKLLAEEKLEVVDFTSYVDRSTEPGHYVIFWEMS 490
           CRR+L+L+INIDKNTEKDLQ+AVEEASK L  EKLEV+DFTS+V+RS++PG YVIFWE+S
Sbjct: 427 CRRSLVLSINIDKNTEKDLQLAVEEASKFLEGEKLEVMDFTSFVERSSDPGRYVIFWELS 486

Query: 491 GEAYNDDILKQCCNCLDSSFVDAGYVSSRRVKAIGALELRVVQRGTFQKILDHFLSLGAA 550
           G+A +D++L  C N LD +F+DAGY  SR++K IG LELR++++GTF++ILDHFLSLG A
Sbjct: 487 GDA-SDEVLSSCANALDLAFIDAGYTGSRKIKTIGPLELRILRKGTFKEILDHFLSLGGA 546

Query: 551 VSQFKTPRCIGPANNKVLQILCSNVNKVYFSTAF 585
           VSQFKTPR + P+N+KVLQIL  NV + YFSTA+
Sbjct: 547 VSQFKTPRFVNPSNSKVLQILSRNVTQSYFSTAY 579

BLAST of Spg013797 vs. ExPASy Swiss-Prot
Match: Q9SKE2 (Jasmonoyl--L-amino acid synthetase JAR1 OS=Arabidopsis thaliana OX=3702 GN=JAR1 PE=1 SV=2)

HSP 1 Score: 803.5 bits (2074), Expect = 1.6e-231
Identity = 381/587 (64.91%), Postives = 480/587 (81.77%), Query Frame = 0

Query: 1   MLEKMEEFDMEKVIEEFEELTKDAERVQRETLKMILEENGSAEYLQNLGLNGK-TDP-VS 60
           MLEK+E FDM +VI+EF+E+T++A +VQ++TLK IL +N SA YLQN GLNG  TDP  +
Sbjct: 1   MLEKVETFDMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNCGLNGNATDPEEA 60

Query: 61  FKACVPIVTHEDLEPYIQRIADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELIET 120
           FK+ VP+VT  +LEPYI+R+ DG  +PILTG P+  ISLSSGT+QG+PKF+PF DEL+E 
Sbjct: 61  FKSMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMEN 120

Query: 121 TIQIFRTSFAFRNRDFPI-EKGRSLMFIYGSKQFKTNGDLMAATATSNLYRNPRFKSTMK 180
           T+Q+FRT+FAFRNRDFPI + G++L FI+ SKQ+ + G +   TAT+N+YRNP FK+ MK
Sbjct: 121 TLQLFRTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMK 180

Query: 181 AIMSTTCSPDEVMFGSDFHQSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFENIWEE 240
           +I S +CSPDEV+F  D HQ+LYCHLL G++FRD++Q +F+ FAH +VHAFRTFE +WEE
Sbjct: 181 SITSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEE 240

Query: 241 LCCDIQSGVLSSRITSPSIRAGMSKLLKPNPELANLVYEKCVGLSNWYGLIPQLFPNAKY 300
           +  DI+ GVLS+RIT PS+R  MSKLL PNPELA  +  KC+ LSNWYGLIP LFPNAKY
Sbjct: 241 IVTDIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSLSNWYGLIPALFPNAKY 300

Query: 301 IYGIMTGSMEPYVKKLRHYAGELPLMSADYGSSEGWIGANTNPKMSPEFATFTVLPNIGY 360
           +YGIMTGSMEPYV KLRHYAG+LPL+S DYGSSEGWI AN  P++SPE ATF V+PN+GY
Sbjct: 301 VYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGY 360

Query: 361 FEFIPLGDDIFEHDKTQSSSICNELKPVDLTQVQVGKEYEILITSVAGLYRYRLGDVVKV 420
           FEF+P+           S +   E KPV LTQV++G+EYE++IT+ AGLYRYRLGDVVKV
Sbjct: 361 FEFLPV-----------SETGEGEEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGDVVKV 420

Query: 421 TGFHNSTPVLKFVCRRNLLLTINIDKNTEKDLQIAVEEASKLLAEEKLEVVDFTSYVDRS 480
            GF+N+TP LKF+CRRNL+L+INIDKNTE+DLQ++VE A+K L+EEK+EV+DF+SY+D S
Sbjct: 421 IGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEKIEVIDFSSYIDVS 480

Query: 481 TEPGHYVIFWEMSGEAYNDDILKQCCNCLDSSFVDAGYVSSRRVKAIGALELRVVQRGTF 540
           T+PGHY IFWE+SGE  N+D+L+ CCNCLD +F+DAGYVSSR+ K IGALELRVV +GTF
Sbjct: 481 TDPGHYAIFWEISGET-NEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAKGTF 540

Query: 541 QKILDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVNKVYFSTAF 585
           +KI +HFL LG++  QFK PRC+ P+N KVLQILC NV   YFSTAF
Sbjct: 541 RKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 575

BLAST of Spg013797 vs. ExPASy Swiss-Prot
Match: Q53P49 (Probable indole-3-acetic acid-amido synthetase GH3.12 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.12 PE=2 SV=1)

HSP 1 Score: 614.0 bits (1582), Expect = 1.8e-174
Identity = 311/610 (50.98%), Postives = 423/610 (69.34%), Query Frame = 0

Query: 1   MLEKMEEFDMEKVIEE----FEELTKDAERVQRETLKMILEENGSAEYLQNLGLNGKTDP 60
           MLEK  +  M +  EE    FE  T+DA  VQRETL+ IL EN   EYL+ LGL G TD 
Sbjct: 1   MLEKEGKLIMSREDEEIMAWFERTTRDAADVQRETLRRILAENAGVEYLRELGLAGLTDA 60

Query: 61  VSFKACVPIVTHEDLEPYIQRIADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELI 120
            SF+A VP+VTH DL+PYIQR+ADG  +P+LT KP+T ISLSSGTTQGK K + FND+L+
Sbjct: 61  GSFRARVPVVTHADLDPYIQRVADGDASPVLTAKPVTAISLSSGTTQGKRKRLLFNDDLL 120

Query: 121 ETTIQIFRTSFAFRNRDFPIEKGRSLMFIYGSKQFKTNGDLMAATATSNLYRNPRFKSTM 180
            ++I+ F  S+AF NR FP+E GR L F+YGS+   T G L A T  +NL R+  F ++M
Sbjct: 121 RSSIRFFHASYAFTNRAFPVEDGRVLQFMYGSRHETTKGGLTATTVMTNLLRSEEFTASM 180

Query: 181 KAIMS---TTCSPDEVMFGSDFHQSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFEN 240
            A       +CSP EV+F  DF +SLYCHLLCGL+   E++ + ++FAHSIV A +  E 
Sbjct: 181 AARSRPRLPSCSPSEVVFSPDFDESLYCHLLCGLLLAGEVRAVSASFAHSIVVALQALER 240

Query: 241 IWEELCCDIQSGVLS-SRITSPSIRAGMSKLL-KPNPELANLVYEKCVGLSNWYGLIPQL 300
           +W ELC DI+ G  S +R+T+P++R  ++ +L  PNP LA+ +  +C  L +W G+IP L
Sbjct: 241 VWRELCADIRRGAASPARVTTPAVRRAVAPILAAPNPALADALERRCAALGDWSGVIPAL 300

Query: 301 FPNAKYIYGIMTGSMEPYVKKLRHYAGELPLMSADYGSSEGWIGANTNPKMSPEFATFTV 360
           +PNA+Y+   MTGSME YVKKLRHYAG +PL+S +Y SSEG IG N      PE   FTV
Sbjct: 301 WPNARYVQATMTGSMEHYVKKLRHYAGGVPLVSGNYASSEGVIGINAEQHAPPESVVFTV 360

Query: 361 LPNIGYFEFIPL----GDDIFEHDK-TQSSSIC----NELKPVDLTQVQVGKEYEILITS 420
           LP+  YFEFIPL     D   + D    + S C    ++  PV LT V VG+ YE+++T+
Sbjct: 361 LPDAAYFEFIPLKPPCTDAAADDDNPAAAGSSCYVDADDANPVGLTDVVVGEHYEVVMTT 420

Query: 421 VAGLYRYRLGDVVKVTGFHNSTPVLKFVCRRNLLLTINIDKNTEKDLQIAVEEASKLLA- 480
             GLYRYRLGDVVKV GFH++TP L+FVCRR+L+L+IN+DKN+E DLQ+AV+ A+K+LA 
Sbjct: 421 FTGLYRYRLGDVVKVAGFHHATPKLRFVCRRSLILSINVDKNSEHDLQLAVDSAAKILAG 480

Query: 481 ----EEKLEVVDFTSYVDRSTEPGHYVIFWEMSGEAYND--DILKQCCNCLDSSF-VDAG 540
                ++LE+ D+TS+ D S++PGHYV+FWE++G    D   +L++CC+ +D +F  DAG
Sbjct: 481 DGENHKQLEIADYTSHADTSSDPGHYVVFWELNGGGEEDGGGVLQRCCDEMDRAFGADAG 540

Query: 541 YVSSRRVKAIGALELRVVQRGTFQKILDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSN 585
           Y  SR+  AIGALELRV++RG FQ++L H+++ G++  QFK PRC+ P+N  VL++L  N
Sbjct: 541 YAQSRKTCAIGALELRVLRRGAFQEVLRHYVAGGSSAGQFKMPRCVAPSNAGVLRVLKDN 600

BLAST of Spg013797 vs. ExPASy TrEMBL
Match: A0A6J1DWL3 (jasmonic acid-amido synthetase JAR1-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111025068 PE=3 SV=1)

HSP 1 Score: 1059.7 bits (2739), Expect = 4.5e-306
Identity = 522/585 (89.23%), Postives = 557/585 (95.21%), Query Frame = 0

Query: 1   MLEKMEEFDMEKVIEEFEELTKDAERVQRETLKMILEENGSAEYLQNLGLNGKTDPVSFK 60
           MLEKMEEFD EKVIEE E ++K+A RVQRETLK ILEENGS+EYLQNLGLNG TDP SFK
Sbjct: 1   MLEKMEEFDTEKVIEEVEAMSKEAGRVQRETLKRILEENGSSEYLQNLGLNGNTDPESFK 60

Query: 61  ACVPIVTHEDLEPYIQRIADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELIETTI 120
           ACVPIVTHEDLEPYIQRIAD   A ILT KPITTISLSSGTT+GKPKFVPFNDEL+++TI
Sbjct: 61  ACVPIVTHEDLEPYIQRIADSHHASILTAKPITTISLSSGTTEGKPKFVPFNDELVDSTI 120

Query: 121 QIFRTSFAFRNRDFPIEKGRSLMFIYGSKQFKTNGDLMAATATSNLYRNPRFKSTMKAIM 180
           QIF+ SFAFRNRDFPI KGRSLMFIYGSKQFKTNG L AATATSNLYR+P+FKSTMK+IM
Sbjct: 121 QIFQASFAFRNRDFPIGKGRSLMFIYGSKQFKTNGGLTAATATSNLYRDPKFKSTMKSIM 180

Query: 181 STTCSPDEVMFGSDFHQSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFENIWEELCC 240
           S +CSPDEV++GSDFHQSLYCHLLCGLIFRDEIQ+IFSTFAHSIVHAFRTFE+IWEELCC
Sbjct: 181 SMSCSPDEVIYGSDFHQSLYCHLLCGLIFRDEIQLIFSTFAHSIVHAFRTFEHIWEELCC 240

Query: 241 DIQSGVLSSRITSPSIRAGMSKLLKPNPELANLVYEKCVGLSNWYGLIPQLFPNAKYIYG 300
           DI++G+L+SRITSPSIRAGMS LLKPNPELA+LVYEKCVGLS WYGLIPQLFPNAKYIYG
Sbjct: 241 DIRNGILTSRITSPSIRAGMSNLLKPNPELADLVYEKCVGLSTWYGLIPQLFPNAKYIYG 300

Query: 301 IMTGSMEPYVKKLRHYAGELPLMSADYGSSEGWIGANTNPKMSPEFATFTVLPNIGYFEF 360
           IMTGSMEPYVKKLRHYAG+LPLMSADYGSSEGWIGAN NPK+SPEFATFTVLPNIGYFEF
Sbjct: 301 IMTGSMEPYVKKLRHYAGDLPLMSADYGSSEGWIGANINPKLSPEFATFTVLPNIGYFEF 360

Query: 361 IPLGDDIFEHDKTQSSSICNELKPVDLTQVQVGKEYEILITSVAGLYRYRLGDVVKVTGF 420
           IPLG +I E+DK+QSS IC+ELKPVDLTQVQVGKEYEILITSVAG+YRYRLGDVVKVTGF
Sbjct: 361 IPLG-EISENDKSQSSLICSELKPVDLTQVQVGKEYEILITSVAGMYRYRLGDVVKVTGF 420

Query: 421 HNSTPVLKFVCRRNLLLTINIDKNTEKDLQIAVEEASKLLAEEKLEVVDFTSYVDRSTEP 480
           +NSTP LKFVCRRNLLLTINIDKNTEKDLQIAV EASKLLAEEKLEVVDFTSYVDRST+P
Sbjct: 421 YNSTPELKFVCRRNLLLTINIDKNTEKDLQIAVGEASKLLAEEKLEVVDFTSYVDRSTDP 480

Query: 481 GHYVIFWEMSGEAYNDDILKQCCNCLDSSFVDAGYVSSRRVKAIGALELRVVQRGTFQKI 540
           GHYVIFWEMSGEAYNDD+L +CCN LD+SFVDAGYVSSRRVK+IGALELRVVQRGTFQKI
Sbjct: 481 GHYVIFWEMSGEAYNDDLLNRCCNRLDTSFVDAGYVSSRRVKSIGALELRVVQRGTFQKI 540

Query: 541 LDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVNKVYFSTAFQ 586
           LDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNV KVYFSTAFQ
Sbjct: 541 LDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVIKVYFSTAFQ 584

BLAST of Spg013797 vs. ExPASy TrEMBL
Match: A0A6J1DWB6 (jasmonic acid-amido synthetase JAR1-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111025068 PE=3 SV=1)

HSP 1 Score: 982.2 bits (2538), Expect = 9.1e-283
Identity = 494/585 (84.44%), Postives = 524/585 (89.57%), Query Frame = 0

Query: 1   MLEKMEEFDMEKVIEEFEELTKDAERVQRETLKMILEENGSAEYLQNLGLNGKTDPVSFK 60
           MLEKMEEFD EKVIEE E ++K+A RVQRETLK ILEENGS+EYLQNLGLNG TDP SFK
Sbjct: 1   MLEKMEEFDTEKVIEEVEAMSKEAGRVQRETLKRILEENGSSEYLQNLGLNGNTDPESFK 60

Query: 61  ACVPIVTHEDLEPYIQRIADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELIETTI 120
           ACVPIVTHEDLEPYIQRIAD   A ILT KPITTISL                       
Sbjct: 61  ACVPIVTHEDLEPYIQRIADSHHASILTAKPITTISL----------------------- 120

Query: 121 QIFRTSFAFRNRDFPIEKGRSLMFIYGSKQFKTNGDLMAATATSNLYRNPRFKSTMKAIM 180
                      RDFPI KGRSLMFIYGSKQFKTNG L AATATSNLYR+P+FKSTMK+IM
Sbjct: 121 -----------RDFPIGKGRSLMFIYGSKQFKTNGGLTAATATSNLYRDPKFKSTMKSIM 180

Query: 181 STTCSPDEVMFGSDFHQSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFENIWEELCC 240
           S +CSPDEV++GSDFHQSLYCHLLCGLIFRDEIQ+IFSTFAHSIVHAFRTFE+IWEELCC
Sbjct: 181 SMSCSPDEVIYGSDFHQSLYCHLLCGLIFRDEIQLIFSTFAHSIVHAFRTFEHIWEELCC 240

Query: 241 DIQSGVLSSRITSPSIRAGMSKLLKPNPELANLVYEKCVGLSNWYGLIPQLFPNAKYIYG 300
           DI++G+L+SRITSPSIRAGMS LLKPNPELA+LVYEKCVGLS WYGLIPQLFPNAKYIYG
Sbjct: 241 DIRNGILTSRITSPSIRAGMSNLLKPNPELADLVYEKCVGLSTWYGLIPQLFPNAKYIYG 300

Query: 301 IMTGSMEPYVKKLRHYAGELPLMSADYGSSEGWIGANTNPKMSPEFATFTVLPNIGYFEF 360
           IMTGSMEPYVKKLRHYAG+LPLMSADYGSSEGWIGAN NPK+SPEFATFTVLPNIGYFEF
Sbjct: 301 IMTGSMEPYVKKLRHYAGDLPLMSADYGSSEGWIGANINPKLSPEFATFTVLPNIGYFEF 360

Query: 361 IPLGDDIFEHDKTQSSSICNELKPVDLTQVQVGKEYEILITSVAGLYRYRLGDVVKVTGF 420
           IPLG +I E+DK+QSS IC+ELKPVDLTQVQVGKEYEILITSVAG+YRYRLGDVVKVTGF
Sbjct: 361 IPLG-EISENDKSQSSLICSELKPVDLTQVQVGKEYEILITSVAGMYRYRLGDVVKVTGF 420

Query: 421 HNSTPVLKFVCRRNLLLTINIDKNTEKDLQIAVEEASKLLAEEKLEVVDFTSYVDRSTEP 480
           +NSTP LKFVCRRNLLLTINIDKNTEKDLQIAV EASKLLAEEKLEVVDFTSYVDRST+P
Sbjct: 421 YNSTPELKFVCRRNLLLTINIDKNTEKDLQIAVGEASKLLAEEKLEVVDFTSYVDRSTDP 480

Query: 481 GHYVIFWEMSGEAYNDDILKQCCNCLDSSFVDAGYVSSRRVKAIGALELRVVQRGTFQKI 540
           GHYVIFWEMSGEAYNDD+L +CCN LD+SFVDAGYVSSRRVK+IGALELRVVQRGTFQKI
Sbjct: 481 GHYVIFWEMSGEAYNDDLLNRCCNRLDTSFVDAGYVSSRRVKSIGALELRVVQRGTFQKI 540

Query: 541 LDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVNKVYFSTAFQ 586
           LDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNV KVYFSTAFQ
Sbjct: 541 LDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVIKVYFSTAFQ 550

BLAST of Spg013797 vs. ExPASy TrEMBL
Match: A0A2I4E0S1 (jasmonoyl--L-amino acid synthetase JAR4-like isoform X2 OS=Juglans regia OX=51240 GN=LOC108985239 PE=3 SV=1)

HSP 1 Score: 943.0 bits (2436), Expect = 6.1e-271
Identity = 453/586 (77.30%), Postives = 522/586 (89.08%), Query Frame = 0

Query: 1   MLEKMEEFDMEKVIEEFEELTKDAERVQRETLKMILEENGSAEYLQNLGLNGKTDPVSFK 60
           MLEKMEEFD EKVIEEFE +T+DA +VQRETLK ILEENGSAEYLQ+LGLNG+TDP SFK
Sbjct: 11  MLEKMEEFDPEKVIEEFEAITRDAGKVQRETLKKILEENGSAEYLQSLGLNGRTDPESFK 70

Query: 61  ACVPIVTHEDLEPYIQRIADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELIETTI 120
            CVP+VTH++LEPYI RIADG  +PILTGKPITTISLSSGTTQGKPKFVPFNDEL+E+T+
Sbjct: 71  TCVPLVTHKELEPYIHRIADGDPSPILTGKPITTISLSSGTTQGKPKFVPFNDELMESTM 130

Query: 121 QIFRTSFAFRNRDFPIEKGRSLMFIYGSKQFKTNGDLMAATATSNLYRNPRFKSTMKAIM 180
           QI+RTSFAFRNR+FPI  G+ L FIY SKQF+T G L A TAT+N+YRN +FK TMKAI 
Sbjct: 131 QIYRTSFAFRNREFPIGNGKGLQFIYSSKQFQTKGGLAAGTATTNVYRNSQFKETMKAIQ 190

Query: 181 STTCSPDEVMFGSDFHQSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFENIWEELCC 240
           S +C PDEV+FG DFHQSLYCHLLCGLIFRDE+Q +FSTFAHSI+HAFRTFE +WEELC 
Sbjct: 191 SQSCCPDEVIFGPDFHQSLYCHLLCGLIFRDEVQFVFSTFAHSILHAFRTFEQVWEELCT 250

Query: 241 DIQSGVLSSRITSPSIRAGMSKLLKPNPELANLVYEKCVGLSNWYGLIPQLFPNAKYIYG 300
           DI+ GVLS+R+++PSIRA MSKLLKPNPELA+LVY+K  GLSNWY LIP+LFPNAKYIYG
Sbjct: 251 DIREGVLSNRVSAPSIRAAMSKLLKPNPELADLVYKKITGLSNWYRLIPELFPNAKYIYG 310

Query: 301 IMTGSMEPYVKKLRHYAGELPLMSADYGSSEGWIGANTNPKMSPEFATFTVLPNIGYFEF 360
           IMTGSMEPY++KLRHYAGELPL+SADYGSSEGWIGAN NPK+ PE ATF VLPNIG+FEF
Sbjct: 311 IMTGSMEPYLRKLRHYAGELPLLSADYGSSEGWIGANVNPKLPPESATFAVLPNIGFFEF 370

Query: 361 IPLGDDIFEHDKTQ--SSSICNELKPVDLTQVQVGKEYEILITSVAGLYRYRLGDVVKVT 420
           IPL  +I + D+ Q   S    E +PVDLT+V++G++YEI++T+ AGLYRY+LGDVVKV 
Sbjct: 371 IPLKHNIEDQDQNQMDPSFFQIEHEPVDLTEVKIGEDYEIIVTNFAGLYRYKLGDVVKVM 430

Query: 421 GFHNSTPVLKFVCRRNLLLTINIDKNTEKDLQIAVEEASKLLAEEKLEVVDFTSYVDRST 480
           GFHNSTP LKFVCRRNLLLTINIDKNTEKDLQ+AVEEASKLL EEKLEVVD++S+VD ST
Sbjct: 431 GFHNSTPELKFVCRRNLLLTINIDKNTEKDLQLAVEEASKLLGEEKLEVVDYSSHVDSST 490

Query: 481 EPGHYVIFWEMSGEAYNDDILKQCCNCLDSSFVDAGYVSSRRVKAIGALELRVVQRGTFQ 540
           +PGHYVIFWE+SGEA  +++LK+CCNCLD SF+DAGYVSSR+V AIGALELRVV+RGTFQ
Sbjct: 491 DPGHYVIFWEISGEA-GEEVLKECCNCLDRSFIDAGYVSSRKVNAIGALELRVVRRGTFQ 550

Query: 541 KILDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVNKVYFSTAF 585
           KILDH+L LG+A+SQFKTPRC+GP NN VLQILC NV + YFSTAF
Sbjct: 551 KILDHYLGLGSALSQFKTPRCVGPTNNLVLQILCGNVVESYFSTAF 595

BLAST of Spg013797 vs. ExPASy TrEMBL
Match: A0A6P9E9C2 (jasmonoyl--L-amino acid synthetase JAR4-like isoform X1 OS=Juglans regia OX=51240 GN=LOC108985239 PE=3 SV=1)

HSP 1 Score: 943.0 bits (2436), Expect = 6.1e-271
Identity = 453/586 (77.30%), Postives = 522/586 (89.08%), Query Frame = 0

Query: 1   MLEKMEEFDMEKVIEEFEELTKDAERVQRETLKMILEENGSAEYLQNLGLNGKTDPVSFK 60
           MLEKMEEFD EKVIEEFE +T+DA +VQRETLK ILEENGSAEYLQ+LGLNG+TDP SFK
Sbjct: 46  MLEKMEEFDPEKVIEEFEAITRDAGKVQRETLKKILEENGSAEYLQSLGLNGRTDPESFK 105

Query: 61  ACVPIVTHEDLEPYIQRIADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELIETTI 120
            CVP+VTH++LEPYI RIADG  +PILTGKPITTISLSSGTTQGKPKFVPFNDEL+E+T+
Sbjct: 106 TCVPLVTHKELEPYIHRIADGDPSPILTGKPITTISLSSGTTQGKPKFVPFNDELMESTM 165

Query: 121 QIFRTSFAFRNRDFPIEKGRSLMFIYGSKQFKTNGDLMAATATSNLYRNPRFKSTMKAIM 180
           QI+RTSFAFRNR+FPI  G+ L FIY SKQF+T G L A TAT+N+YRN +FK TMKAI 
Sbjct: 166 QIYRTSFAFRNREFPIGNGKGLQFIYSSKQFQTKGGLAAGTATTNVYRNSQFKETMKAIQ 225

Query: 181 STTCSPDEVMFGSDFHQSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFENIWEELCC 240
           S +C PDEV+FG DFHQSLYCHLLCGLIFRDE+Q +FSTFAHSI+HAFRTFE +WEELC 
Sbjct: 226 SQSCCPDEVIFGPDFHQSLYCHLLCGLIFRDEVQFVFSTFAHSILHAFRTFEQVWEELCT 285

Query: 241 DIQSGVLSSRITSPSIRAGMSKLLKPNPELANLVYEKCVGLSNWYGLIPQLFPNAKYIYG 300
           DI+ GVLS+R+++PSIRA MSKLLKPNPELA+LVY+K  GLSNWY LIP+LFPNAKYIYG
Sbjct: 286 DIREGVLSNRVSAPSIRAAMSKLLKPNPELADLVYKKITGLSNWYRLIPELFPNAKYIYG 345

Query: 301 IMTGSMEPYVKKLRHYAGELPLMSADYGSSEGWIGANTNPKMSPEFATFTVLPNIGYFEF 360
           IMTGSMEPY++KLRHYAGELPL+SADYGSSEGWIGAN NPK+ PE ATF VLPNIG+FEF
Sbjct: 346 IMTGSMEPYLRKLRHYAGELPLLSADYGSSEGWIGANVNPKLPPESATFAVLPNIGFFEF 405

Query: 361 IPLGDDIFEHDKTQ--SSSICNELKPVDLTQVQVGKEYEILITSVAGLYRYRLGDVVKVT 420
           IPL  +I + D+ Q   S    E +PVDLT+V++G++YEI++T+ AGLYRY+LGDVVKV 
Sbjct: 406 IPLKHNIEDQDQNQMDPSFFQIEHEPVDLTEVKIGEDYEIIVTNFAGLYRYKLGDVVKVM 465

Query: 421 GFHNSTPVLKFVCRRNLLLTINIDKNTEKDLQIAVEEASKLLAEEKLEVVDFTSYVDRST 480
           GFHNSTP LKFVCRRNLLLTINIDKNTEKDLQ+AVEEASKLL EEKLEVVD++S+VD ST
Sbjct: 466 GFHNSTPELKFVCRRNLLLTINIDKNTEKDLQLAVEEASKLLGEEKLEVVDYSSHVDSST 525

Query: 481 EPGHYVIFWEMSGEAYNDDILKQCCNCLDSSFVDAGYVSSRRVKAIGALELRVVQRGTFQ 540
           +PGHYVIFWE+SGEA  +++LK+CCNCLD SF+DAGYVSSR+V AIGALELRVV+RGTFQ
Sbjct: 526 DPGHYVIFWEISGEA-GEEVLKECCNCLDRSFIDAGYVSSRKVNAIGALELRVVRRGTFQ 585

Query: 541 KILDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVNKVYFSTAF 585
           KILDH+L LG+A+SQFKTPRC+GP NN VLQILC NV + YFSTAF
Sbjct: 586 KILDHYLGLGSALSQFKTPRCVGPTNNLVLQILCGNVVESYFSTAF 630

BLAST of Spg013797 vs. ExPASy TrEMBL
Match: A0A2I4E0R7 (jasmonoyl--L-amino acid synthetase JAR4-like isoform X3 OS=Juglans regia OX=51240 GN=LOC108985239 PE=3 SV=1)

HSP 1 Score: 943.0 bits (2436), Expect = 6.1e-271
Identity = 453/586 (77.30%), Postives = 522/586 (89.08%), Query Frame = 0

Query: 1   MLEKMEEFDMEKVIEEFEELTKDAERVQRETLKMILEENGSAEYLQNLGLNGKTDPVSFK 60
           MLEKMEEFD EKVIEEFE +T+DA +VQRETLK ILEENGSAEYLQ+LGLNG+TDP SFK
Sbjct: 1   MLEKMEEFDPEKVIEEFEAITRDAGKVQRETLKKILEENGSAEYLQSLGLNGRTDPESFK 60

Query: 61  ACVPIVTHEDLEPYIQRIADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELIETTI 120
            CVP+VTH++LEPYI RIADG  +PILTGKPITTISLSSGTTQGKPKFVPFNDEL+E+T+
Sbjct: 61  TCVPLVTHKELEPYIHRIADGDPSPILTGKPITTISLSSGTTQGKPKFVPFNDELMESTM 120

Query: 121 QIFRTSFAFRNRDFPIEKGRSLMFIYGSKQFKTNGDLMAATATSNLYRNPRFKSTMKAIM 180
           QI+RTSFAFRNR+FPI  G+ L FIY SKQF+T G L A TAT+N+YRN +FK TMKAI 
Sbjct: 121 QIYRTSFAFRNREFPIGNGKGLQFIYSSKQFQTKGGLAAGTATTNVYRNSQFKETMKAIQ 180

Query: 181 STTCSPDEVMFGSDFHQSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFENIWEELCC 240
           S +C PDEV+FG DFHQSLYCHLLCGLIFRDE+Q +FSTFAHSI+HAFRTFE +WEELC 
Sbjct: 181 SQSCCPDEVIFGPDFHQSLYCHLLCGLIFRDEVQFVFSTFAHSILHAFRTFEQVWEELCT 240

Query: 241 DIQSGVLSSRITSPSIRAGMSKLLKPNPELANLVYEKCVGLSNWYGLIPQLFPNAKYIYG 300
           DI+ GVLS+R+++PSIRA MSKLLKPNPELA+LVY+K  GLSNWY LIP+LFPNAKYIYG
Sbjct: 241 DIREGVLSNRVSAPSIRAAMSKLLKPNPELADLVYKKITGLSNWYRLIPELFPNAKYIYG 300

Query: 301 IMTGSMEPYVKKLRHYAGELPLMSADYGSSEGWIGANTNPKMSPEFATFTVLPNIGYFEF 360
           IMTGSMEPY++KLRHYAGELPL+SADYGSSEGWIGAN NPK+ PE ATF VLPNIG+FEF
Sbjct: 301 IMTGSMEPYLRKLRHYAGELPLLSADYGSSEGWIGANVNPKLPPESATFAVLPNIGFFEF 360

Query: 361 IPLGDDIFEHDKTQ--SSSICNELKPVDLTQVQVGKEYEILITSVAGLYRYRLGDVVKVT 420
           IPL  +I + D+ Q   S    E +PVDLT+V++G++YEI++T+ AGLYRY+LGDVVKV 
Sbjct: 361 IPLKHNIEDQDQNQMDPSFFQIEHEPVDLTEVKIGEDYEIIVTNFAGLYRYKLGDVVKVM 420

Query: 421 GFHNSTPVLKFVCRRNLLLTINIDKNTEKDLQIAVEEASKLLAEEKLEVVDFTSYVDRST 480
           GFHNSTP LKFVCRRNLLLTINIDKNTEKDLQ+AVEEASKLL EEKLEVVD++S+VD ST
Sbjct: 421 GFHNSTPELKFVCRRNLLLTINIDKNTEKDLQLAVEEASKLLGEEKLEVVDYSSHVDSST 480

Query: 481 EPGHYVIFWEMSGEAYNDDILKQCCNCLDSSFVDAGYVSSRRVKAIGALELRVVQRGTFQ 540
           +PGHYVIFWE+SGEA  +++LK+CCNCLD SF+DAGYVSSR+V AIGALELRVV+RGTFQ
Sbjct: 481 DPGHYVIFWEISGEA-GEEVLKECCNCLDRSFIDAGYVSSRKVNAIGALELRVVRRGTFQ 540

Query: 541 KILDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVNKVYFSTAF 585
           KILDH+L LG+A+SQFKTPRC+GP NN VLQILC NV + YFSTAF
Sbjct: 541 KILDHYLGLGSALSQFKTPRCVGPTNNLVLQILCGNVVESYFSTAF 585

BLAST of Spg013797 vs. TAIR 10
Match: AT2G46370.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 803.5 bits (2074), Expect = 1.1e-232
Identity = 381/587 (64.91%), Postives = 480/587 (81.77%), Query Frame = 0

Query: 1   MLEKMEEFDMEKVIEEFEELTKDAERVQRETLKMILEENGSAEYLQNLGLNGK-TDP-VS 60
           MLEK+E FDM +VI+EF+E+T++A +VQ++TLK IL +N SA YLQN GLNG  TDP  +
Sbjct: 1   MLEKVETFDMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNCGLNGNATDPEEA 60

Query: 61  FKACVPIVTHEDLEPYIQRIADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELIET 120
           FK+ VP+VT  +LEPYI+R+ DG  +PILTG P+  ISLSSGT+QG+PKF+PF DEL+E 
Sbjct: 61  FKSMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMEN 120

Query: 121 TIQIFRTSFAFRNRDFPI-EKGRSLMFIYGSKQFKTNGDLMAATATSNLYRNPRFKSTMK 180
           T+Q+FRT+FAFRNRDFPI + G++L FI+ SKQ+ + G +   TAT+N+YRNP FK+ MK
Sbjct: 121 TLQLFRTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMK 180

Query: 181 AIMSTTCSPDEVMFGSDFHQSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFENIWEE 240
           +I S +CSPDEV+F  D HQ+LYCHLL G++FRD++Q +F+ FAH +VHAFRTFE +WEE
Sbjct: 181 SITSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEE 240

Query: 241 LCCDIQSGVLSSRITSPSIRAGMSKLLKPNPELANLVYEKCVGLSNWYGLIPQLFPNAKY 300
           +  DI+ GVLS+RIT PS+R  MSKLL PNPELA  +  KC+ LSNWYGLIP LFPNAKY
Sbjct: 241 IVTDIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSLSNWYGLIPALFPNAKY 300

Query: 301 IYGIMTGSMEPYVKKLRHYAGELPLMSADYGSSEGWIGANTNPKMSPEFATFTVLPNIGY 360
           +YGIMTGSMEPYV KLRHYAG+LPL+S DYGSSEGWI AN  P++SPE ATF V+PN+GY
Sbjct: 301 VYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGY 360

Query: 361 FEFIPLGDDIFEHDKTQSSSICNELKPVDLTQVQVGKEYEILITSVAGLYRYRLGDVVKV 420
           FEF+P+           S +   E KPV LTQV++G+EYE++IT+ AGLYRYRLGDVVKV
Sbjct: 361 FEFLPV-----------SETGEGEEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGDVVKV 420

Query: 421 TGFHNSTPVLKFVCRRNLLLTINIDKNTEKDLQIAVEEASKLLAEEKLEVVDFTSYVDRS 480
            GF+N+TP LKF+CRRNL+L+INIDKNTE+DLQ++VE A+K L+EEK+EV+DF+SY+D S
Sbjct: 421 IGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEKIEVIDFSSYIDVS 480

Query: 481 TEPGHYVIFWEMSGEAYNDDILKQCCNCLDSSFVDAGYVSSRRVKAIGALELRVVQRGTF 540
           T+PGHY IFWE+SGE  N+D+L+ CCNCLD +F+DAGYVSSR+ K IGALELRVV +GTF
Sbjct: 481 TDPGHYAIFWEISGET-NEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAKGTF 540

Query: 541 QKILDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVNKVYFSTAF 585
           +KI +HFL LG++  QFK PRC+ P+N KVLQILC NV   YFSTAF
Sbjct: 541 RKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 575

BLAST of Spg013797 vs. TAIR 10
Match: AT2G46370.2 (Auxin-responsive GH3 family protein )

HSP 1 Score: 803.5 bits (2074), Expect = 1.1e-232
Identity = 381/587 (64.91%), Postives = 480/587 (81.77%), Query Frame = 0

Query: 1   MLEKMEEFDMEKVIEEFEELTKDAERVQRETLKMILEENGSAEYLQNLGLNGK-TDP-VS 60
           MLEK+E FDM +VI+EF+E+T++A +VQ++TLK IL +N SA YLQN GLNG  TDP  +
Sbjct: 1   MLEKVETFDMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNCGLNGNATDPEEA 60

Query: 61  FKACVPIVTHEDLEPYIQRIADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELIET 120
           FK+ VP+VT  +LEPYI+R+ DG  +PILTG P+  ISLSSGT+QG+PKF+PF DEL+E 
Sbjct: 61  FKSMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMEN 120

Query: 121 TIQIFRTSFAFRNRDFPI-EKGRSLMFIYGSKQFKTNGDLMAATATSNLYRNPRFKSTMK 180
           T+Q+FRT+FAFRNRDFPI + G++L FI+ SKQ+ + G +   TAT+N+YRNP FK+ MK
Sbjct: 121 TLQLFRTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMK 180

Query: 181 AIMSTTCSPDEVMFGSDFHQSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFENIWEE 240
           +I S +CSPDEV+F  D HQ+LYCHLL G++FRD++Q +F+ FAH +VHAFRTFE +WEE
Sbjct: 181 SITSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEE 240

Query: 241 LCCDIQSGVLSSRITSPSIRAGMSKLLKPNPELANLVYEKCVGLSNWYGLIPQLFPNAKY 300
           +  DI+ GVLS+RIT PS+R  MSKLL PNPELA  +  KC+ LSNWYGLIP LFPNAKY
Sbjct: 241 IVTDIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSLSNWYGLIPALFPNAKY 300

Query: 301 IYGIMTGSMEPYVKKLRHYAGELPLMSADYGSSEGWIGANTNPKMSPEFATFTVLPNIGY 360
           +YGIMTGSMEPYV KLRHYAG+LPL+S DYGSSEGWI AN  P++SPE ATF V+PN+GY
Sbjct: 301 VYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGY 360

Query: 361 FEFIPLGDDIFEHDKTQSSSICNELKPVDLTQVQVGKEYEILITSVAGLYRYRLGDVVKV 420
           FEF+P+           S +   E KPV LTQV++G+EYE++IT+ AGLYRYRLGDVVKV
Sbjct: 361 FEFLPV-----------SETGEGEEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGDVVKV 420

Query: 421 TGFHNSTPVLKFVCRRNLLLTINIDKNTEKDLQIAVEEASKLLAEEKLEVVDFTSYVDRS 480
            GF+N+TP LKF+CRRNL+L+INIDKNTE+DLQ++VE A+K L+EEK+EV+DF+SY+D S
Sbjct: 421 IGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEKIEVIDFSSYIDVS 480

Query: 481 TEPGHYVIFWEMSGEAYNDDILKQCCNCLDSSFVDAGYVSSRRVKAIGALELRVVQRGTF 540
           T+PGHY IFWE+SGE  N+D+L+ CCNCLD +F+DAGYVSSR+ K IGALELRVV +GTF
Sbjct: 481 TDPGHYAIFWEISGET-NEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAKGTF 540

Query: 541 QKILDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVNKVYFSTAF 585
           +KI +HFL LG++  QFK PRC+ P+N KVLQILC NV   YFSTAF
Sbjct: 541 RKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 575

BLAST of Spg013797 vs. TAIR 10
Match: AT2G46370.4 (Auxin-responsive GH3 family protein )

HSP 1 Score: 803.5 bits (2074), Expect = 1.1e-232
Identity = 381/587 (64.91%), Postives = 480/587 (81.77%), Query Frame = 0

Query: 1   MLEKMEEFDMEKVIEEFEELTKDAERVQRETLKMILEENGSAEYLQNLGLNGK-TDP-VS 60
           MLEK+E FDM +VI+EF+E+T++A +VQ++TLK IL +N SA YLQN GLNG  TDP  +
Sbjct: 12  MLEKVETFDMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNCGLNGNATDPEEA 71

Query: 61  FKACVPIVTHEDLEPYIQRIADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELIET 120
           FK+ VP+VT  +LEPYI+R+ DG  +PILTG P+  ISLSSGT+QG+PKF+PF DEL+E 
Sbjct: 72  FKSMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMEN 131

Query: 121 TIQIFRTSFAFRNRDFPI-EKGRSLMFIYGSKQFKTNGDLMAATATSNLYRNPRFKSTMK 180
           T+Q+FRT+FAFRNRDFPI + G++L FI+ SKQ+ + G +   TAT+N+YRNP FK+ MK
Sbjct: 132 TLQLFRTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMK 191

Query: 181 AIMSTTCSPDEVMFGSDFHQSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFENIWEE 240
           +I S +CSPDEV+F  D HQ+LYCHLL G++FRD++Q +F+ FAH +VHAFRTFE +WEE
Sbjct: 192 SITSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEE 251

Query: 241 LCCDIQSGVLSSRITSPSIRAGMSKLLKPNPELANLVYEKCVGLSNWYGLIPQLFPNAKY 300
           +  DI+ GVLS+RIT PS+R  MSKLL PNPELA  +  KC+ LSNWYGLIP LFPNAKY
Sbjct: 252 IVTDIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSLSNWYGLIPALFPNAKY 311

Query: 301 IYGIMTGSMEPYVKKLRHYAGELPLMSADYGSSEGWIGANTNPKMSPEFATFTVLPNIGY 360
           +YGIMTGSMEPYV KLRHYAG+LPL+S DYGSSEGWI AN  P++SPE ATF V+PN+GY
Sbjct: 312 VYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGY 371

Query: 361 FEFIPLGDDIFEHDKTQSSSICNELKPVDLTQVQVGKEYEILITSVAGLYRYRLGDVVKV 420
           FEF+P+           S +   E KPV LTQV++G+EYE++IT+ AGLYRYRLGDVVKV
Sbjct: 372 FEFLPV-----------SETGEGEEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGDVVKV 431

Query: 421 TGFHNSTPVLKFVCRRNLLLTINIDKNTEKDLQIAVEEASKLLAEEKLEVVDFTSYVDRS 480
            GF+N+TP LKF+CRRNL+L+INIDKNTE+DLQ++VE A+K L+EEK+EV+DF+SY+D S
Sbjct: 432 IGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEKIEVIDFSSYIDVS 491

Query: 481 TEPGHYVIFWEMSGEAYNDDILKQCCNCLDSSFVDAGYVSSRRVKAIGALELRVVQRGTF 540
           T+PGHY IFWE+SGE  N+D+L+ CCNCLD +F+DAGYVSSR+ K IGALELRVV +GTF
Sbjct: 492 TDPGHYAIFWEISGET-NEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAKGTF 551

Query: 541 QKILDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVNKVYFSTAF 585
           +KI +HFL LG++  QFK PRC+ P+N KVLQILC NV   YFSTAF
Sbjct: 552 RKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 586

BLAST of Spg013797 vs. TAIR 10
Match: AT2G46370.3 (Auxin-responsive GH3 family protein )

HSP 1 Score: 719.9 bits (1857), Expect = 1.6e-207
Identity = 333/508 (65.55%), Postives = 416/508 (81.89%), Query Frame = 0

Query: 78  IADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELIETTIQIFRTSFAFRNRDFPI- 137
           + DG  +PILTG P+  ISLSSGT+QG+PKF+PF DEL+E T+Q+FRT+FAFRNRDFPI 
Sbjct: 1   MVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMENTLQLFRTAFAFRNRDFPID 60

Query: 138 EKGRSLMFIYGSKQFKTNGDLMAATATSNLYRNPRFKSTMKAIMSTTCSPDEVMFGSDFH 197
           + G++L FI+ SKQ+ + G +   TAT+N+YRNP FK+ MK+I S +CSPDEV+F  D H
Sbjct: 61  DNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMKSITSPSCSPDEVIFSPDVH 120

Query: 198 QSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFENIWEELCCDIQSGVLSSRITSPSI 257
           Q+LYCHLL G++FRD++Q +F+ FAH +VHAFRTFE +WEE+  DI+ GVLS+RIT PS+
Sbjct: 121 QALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEEIVTDIKDGVLSNRITVPSV 180

Query: 258 RAGMSKLLKPNPELANLVYEKCVGLSNWYGLIPQLFPNAKYIYGIMTGSMEPYVKKLRHY 317
           R  MSKLL PNPELA  +  KC+ LSNWYGLIP LFPNAKY+YGIMTGSMEPYV KLRHY
Sbjct: 181 RTAMSKLLTPNPELAETIRTKCMSLSNWYGLIPALFPNAKYVYGIMTGSMEPYVPKLRHY 240

Query: 318 AGELPLMSADYGSSEGWIGANTNPKMSPEFATFTVLPNIGYFEFIPLGDDIFEHDKTQSS 377
           AG+LPL+S DYGSSEGWI AN  P++SPE ATF V+PN+GYFEF+P+           S 
Sbjct: 241 AGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGYFEFLPV-----------SE 300

Query: 378 SICNELKPVDLTQVQVGKEYEILITSVAGLYRYRLGDVVKVTGFHNSTPVLKFVCRRNLL 437
           +   E KPV LTQV++G+EYE++IT+ AGLYRYRLGDVVKV GF+N+TP LKF+CRRNL+
Sbjct: 301 TGEGEEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGDVVKVIGFYNNTPQLKFICRRNLI 360

Query: 438 LTINIDKNTEKDLQIAVEEASKLLAEEKLEVVDFTSYVDRSTEPGHYVIFWEMSGEAYND 497
           L+INIDKNTE+DLQ++VE A+K L+EEK+EV+DF+SY+D ST+PGHY IFWE+SGE  N+
Sbjct: 361 LSINIDKNTERDLQLSVESAAKRLSEEKIEVIDFSSYIDVSTDPGHYAIFWEISGET-NE 420

Query: 498 DILKQCCNCLDSSFVDAGYVSSRRVKAIGALELRVVQRGTFQKILDHFLSLGAAVSQFKT 557
           D+L+ CCNCLD +F+DAGYVSSR+ K IGALELRVV +GTF+KI +HFL LG++  QFK 
Sbjct: 421 DVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAKGTFRKIQEHFLGLGSSAGQFKM 480

Query: 558 PRCIGPANNKVLQILCSNVNKVYFSTAF 585
           PRC+ P+N KVLQILC NV   YFSTAF
Sbjct: 481 PRCVKPSNAKVLQILCENVVSSYFSTAF 496

BLAST of Spg013797 vs. TAIR 10
Match: AT4G03400.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 580.5 bits (1495), Expect = 1.5e-165
Identity = 304/591 (51.44%), Postives = 405/591 (68.53%), Query Frame = 0

Query: 11  EKVIEEFEELTKDAERVQRETLKMILEENGSAEYLQN-LG------LNGKTDPVSFKACV 70
           + VI  FE ++++A +VQ ETL+ ILE N   EYL+  LG      ++  T    F + V
Sbjct: 9   DDVIGWFEHVSENACKVQSETLRRILELNSGVEYLRKWLGTVDVEKMDDYTLETLFTSLV 68

Query: 71  PIVTHEDLEPYIQRIADGPCAPILTGKPITTISLSSGTTQGKPKFVPFNDELIETTIQIF 130
           PIV+H DL+PYIQRIADG  +P+LT +PIT +SLSSGTT+G+ K+VPF     +TT+QIF
Sbjct: 69  PIVSHADLDPYIQRIADGETSPLLTQEPITVLSLSSGTTEGRQKYVPFTRHSAQTTLQIF 128

Query: 131 RTSFAFRNRDFPI-EKGRSLMFIYGSKQFKTNGDLMAATATSNLYRNPRFKSTMKAIMST 190
           R S A+R+R +PI E GR L FIY  K+FKT G L   TAT++ Y +  FK+  +   S 
Sbjct: 129 RLSAAYRSRFYPIREGGRILEFIYAGKEFKTLGGLTVGTATTHYYASEEFKTKQETTKSF 188

Query: 191 TCSPDEVMFGSDFHQSLYCHLLCGLIFRDEIQMIFSTFAHSIVHAFRTFENIWEELCCDI 250
           TCSP EV+ G DF Q  YCHLL GL +  +++ + S F+++IV AF  FE IW E+C DI
Sbjct: 189 TCSPQEVISGGDFGQCTYCHLLLGLHYSSQVEFVASAFSYTIVQAFSFFEEIWREICADI 248

Query: 251 QSGVLSSRITSPSIRAGMSKLLKPNPELANLVYEKCVGLS---NWYGLIPQLFPNAKYIY 310
           + G LSSRIT P +R  +  L++PNP LA+ + E C+ L     W+GLI +L+PNAK+I 
Sbjct: 249 KEGNLSSRITLPKMRKAVLALIRPNPSLASHIEEICLELETNLGWFGLISKLWPNAKFIS 308

Query: 311 GIMTGSMEPYVKKLRHYAGELPLMSADYGSSEGWIGANTNPKMSPEFATFTVLPNIGYFE 370
            IMTGSM PY+ KLRHYAG LPL+SADYGS+E WIG N +P + PE  +F V+P   YFE
Sbjct: 309 SIMTGSMLPYLNKLRHYAGGLPLVSADYGSTESWIGVNVDPHLPPEDVSFAVIPTFSYFE 368

Query: 371 FIPLGDDIFEHDKTQSSSIC-----NELKPVDLTQVQVGKEYEILITSVAGLYRYRLGDV 430
           FIPL      + +   S IC      E KPV L+QV++G+EYE+++T+  GLYRYRLGDV
Sbjct: 369 FIPL------YRRQNQSDICIDGDFVEDKPVPLSQVKLGQEYELVLTTFTGLYRYRLGDV 428

Query: 431 VKVTGFHNSTPVLKFVCRRNLLLTINIDKNTEKDLQIAVEEASKLLAEE-KLEVVDFTSY 490
           V+VT FH  TP L F+ RR L+LTINIDKNTEKDLQ  V++AS+LL+   + EVVDFTS+
Sbjct: 429 VEVTSFHKGTPKLSFIYRRKLILTINIDKNTEKDLQRVVDKASQLLSRSTRAEVVDFTSH 488

Query: 491 VDRSTEPGHYVIFWEMSGEAYNDDILKQCCNCLDSSFVDAGYVSSRRVKAIGALELRVVQ 550
            D    PGHYVI+WE+ GEA +D  L++CC  +D++FVD GYV SRR+ +IG LELRVV+
Sbjct: 489 ADVIARPGHYVIYWEIRGEA-DDKALEECCREMDTAFVDYGYVVSRRMNSIGPLELRVVE 548

Query: 551 RGTFQKILDHFLSLGAAVSQFKTPRCIGPANNKVLQILCSNVNKVYFSTAF 585
           RGTF K+ +  +     ++QFKTPRC    N+ +L IL  +  K + S+A+
Sbjct: 549 RGTFGKVAERCVGKCGGLNQFKTPRC--TTNSVMLDILNDSTIKRFRSSAY 590

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022158613.19.3e-30689.23jasmonic acid-amido synthetase JAR1-like isoform X1 [Momordica charantia][more]
XP_022158615.11.9e-28284.44jasmonic acid-amido synthetase JAR1-like isoform X2 [Momordica charantia][more]
XP_041001957.11.0e-27278.16jasmonoyl--L-amino acid synthetase JAR4-like isoform X3 [Juglans microcarpa x Ju... [more]
XP_041001955.11.0e-27278.16jasmonoyl--L-amino acid synthetase JAR4-like isoform X2 [Juglans microcarpa x Ju... [more]
XP_041001954.11.0e-27278.16jasmonoyl--L-amino acid synthetase JAR4-like isoform X1 [Juglans microcarpa x Ju... [more]
Match NameE-valueIdentityDescription
A0A314KSQ46.5e-25472.31Jasmonoyl--L-amino acid synthetase JAR6 OS=Nicotiana attenuata OX=49451 GN=JAR6 ... [more]
A0A1J6KGJ95.5e-25371.75Jasmonoyl--L-amino acid synthetase JAR4 OS=Nicotiana attenuata OX=49451 GN=JAR4 ... [more]
Q6I5816.6e-23866.03Jasmonoyl--L-amino acid synthetase GH3.5 OS=Oryza sativa subsp. japonica OX=3994... [more]
Q9SKE21.6e-23164.91Jasmonoyl--L-amino acid synthetase JAR1 OS=Arabidopsis thaliana OX=3702 GN=JAR1 ... [more]
Q53P491.8e-17450.98Probable indole-3-acetic acid-amido synthetase GH3.12 OS=Oryza sativa subsp. jap... [more]
Match NameE-valueIdentityDescription
A0A6J1DWL34.5e-30689.23jasmonic acid-amido synthetase JAR1-like isoform X1 OS=Momordica charantia OX=36... [more]
A0A6J1DWB69.1e-28384.44jasmonic acid-amido synthetase JAR1-like isoform X2 OS=Momordica charantia OX=36... [more]
A0A2I4E0S16.1e-27177.30jasmonoyl--L-amino acid synthetase JAR4-like isoform X2 OS=Juglans regia OX=5124... [more]
A0A6P9E9C26.1e-27177.30jasmonoyl--L-amino acid synthetase JAR4-like isoform X1 OS=Juglans regia OX=5124... [more]
A0A2I4E0R76.1e-27177.30jasmonoyl--L-amino acid synthetase JAR4-like isoform X3 OS=Juglans regia OX=5124... [more]
Match NameE-valueIdentityDescription
AT2G46370.11.1e-23264.91Auxin-responsive GH3 family protein [more]
AT2G46370.21.1e-23264.91Auxin-responsive GH3 family protein [more]
AT2G46370.41.1e-23264.91Auxin-responsive GH3 family protein [more]
AT2G46370.31.6e-20765.55Auxin-responsive GH3 family protein [more]
AT4G03400.11.5e-16551.44Auxin-responsive GH3 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 10..30
NoneNo IPR availableCOILSCoilCoilcoord: 446..466
NoneNo IPR availablePANTHERPTHR31901:SF40JASMONIC ACID-AMIDO SYNTHETASE JAR1-LIKE ISOFORM X1coord: 6..569
IPR004993GH3 familyPFAMPF03321GH3coord: 16..560
e-value: 1.5E-184
score: 614.7
IPR004993GH3 familyPANTHERPTHR31901GH3 DOMAIN-CONTAINING PROTEINcoord: 6..569

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg013797.1Spg013797.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016881 acid-amino acid ligase activity
molecular_function GO:0102053 (-)-jasmonoyl-isoleucine synthetase activity
molecular_function GO:0102058 jasmonoyl-leucine synthetase activity
molecular_function GO:0102057 jasmonoyl-valine synthetase activity