Spg013747 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg013747 vs. NCBI nr
Match: KAG7030212.1 (hypothetical protein SDJN02_08559, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 470.7 bits (1210), Expect = 1.2e-128
Identity = 251/370 (67.84%), Postives = 282/370 (76.22%), Query Frame = 0

Query: 1   MSFSKLMASFLFLAFISFSS-SLVSAQKALTAYDILQQYGFPVGILPVGVTGYELNRATG 60
           MSFSKL+  FL LAFIS SS  L  AQK+L+AYDILQQYGFPVGILPVGVTGYE N+ATG
Sbjct: 1   MSFSKLLVPFLLLAFISLSSIPLACAQKSLSAYDILQQYGFPVGILPVGVTGYEFNKATG 60

Query: 61  EFSLFLNQKCKFSIDSYELEYKPTINGVISQGRLRKLKGVSVKVVLLWLSIVEVVNDGDD 120
           EFSLFLN+KC+F IDSYELEYKPT+ GVISQG ++ LKGVSVK++L+W +IVEVV+D D+
Sbjct: 61  EFSLFLNRKCRFWIDSYELEYKPTVKGVISQGVIKNLKGVSVKILLIWFNIVEVVSDSDE 120

Query: 121 LQFSVGIASANFPIDSFYESPQCGCGFDCNKAESLILKNMPSSWSQGAFELLYWYSLDSF 180
           L+FS+G+ASANFPI+SFYESPQCGCGFDC+                              
Sbjct: 121 LRFSIGVASANFPINSFYESPQCGCGFDCD------------------------------ 180

Query: 181 LRELKPQTHIDWRSQLAIAIAIATLATFFHISMSPAAASPSASPSTSDGEAPSVYDILRE 240
               KPQ                 L TFFHISMS AA    ASP+T++GEAPSVYDILRE
Sbjct: 181 ----KPQ---------------PPLPTFFHISMSTAA----ASPATNNGEAPSVYDILRE 240

Query: 241 FNFPIGLLPEGVMGWKLDRTTGKLEAYLNEAFHFSPDDPYELKYKPTISGQISKNKLTDL 300
           FNFPIGLLPEGV+G  LDRTTGKLEAYLNEA  FSP+DPYEL+YK TISGQISKN+LTDL
Sbjct: 241 FNFPIGLLPEGVVGCSLDRTTGKLEAYLNEACQFSPEDPYELRYKATISGQISKNRLTDL 300

Query: 301 KGVSVKFMFFWVNIVEVVRNGDDLQFSIGMATASFPVDNFSEC-PQCEFGVDCKDGKVRK 360
           KGV+VKFMFFWVNIVEVVRNGDDL FS+G+ATASFPVDNFSEC P    G DCK+GK  K
Sbjct: 301 KGVTVKFMFFWVNIVEVVRNGDDLLFSVGVATASFPVDNFSECLPPGGCGADCKNGKGGK 317

Query: 361 IKAKSLVSSA 369
           IK KSLVS A
Sbjct: 361 IKTKSLVSPA 317

BLAST of Spg013747 vs. NCBI nr
Match: KAG7014357.1 (hypothetical protein SDJN02_24534, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 408.7 bits (1049), Expect = 5.4e-110
Identity = 224/368 (60.87%), Postives = 251/368 (68.21%), Query Frame = 0

Query: 1   MSFSKLMASFLFLAFISFSSSLVSAQKALTAYDILQQYGFPVGILPVGVTGYELNRATGE 60
           MSFSKLMAS LFLAF+SFSS LVSAQKALTAYDI+QQYGFPVGILP+GVTGY+L+R TG 
Sbjct: 42  MSFSKLMASLLFLAFVSFSSPLVSAQKALTAYDIIQQYGFPVGILPLGVTGYKLDRDTGS 101

Query: 61  FSLFLNQKCKFSIDSYELEYKPTINGVISQGRLRKLKGVSVKVVLLWLSIVEVVNDGDDL 120
           FSLFL+QKCKF+IDSYELEYKPT+ GVISQG+L KLKGVSVK+++LW SIVEVV+DGD+L
Sbjct: 102 FSLFLDQKCKFAIDSYELEYKPTVTGVISQGKLSKLKGVSVKILILWFSIVEVVSDGDEL 161

Query: 121 QFSVGIASANFPIDSFYESPQCGCGFDCNKAESLILKNMPSSWSQGAFELLYWYSLDSFL 180
            FSVGIASANFPIDSFYESP      +C                                
Sbjct: 162 GFSVGIASANFPIDSFYESPH-----NC-------------------------------- 221

Query: 181 RELKPQTHIDWRSQLAIAIAIATLATFFHISMSPAAASPSASPSTSDGEAPSVYDILREF 240
                                                           +A +VYDILREF
Sbjct: 222 ------------------------------------------------KAATVYDILREF 281

Query: 241 NFPIGLLPEGVMGWKLDRTTGKLEAYLNEAFHFSPDDPYELKYKPTISGQISKNKLTDLK 300
           N PIGLLP+  +  KLDR  GKLE +L  + HFS  +PYELKYK T+SG IS NKLT+LK
Sbjct: 282 NLPIGLLPDCAVDCKLDRGKGKLEVHLKGSCHFSLQEPYELKYKSTVSGHISGNKLTNLK 324

Query: 301 GVSVKFMFFWVNIVEVVRNGDDLQFSIGMATASFPVDNFSECPQCEFGVDCKDGKVRKIK 360
           GVSVKFMFFWVNIVEVVRNGDDLQFS+GM TASFPVD F+ CPQ   GVDCKDGK+RKIK
Sbjct: 342 GVSVKFMFFWVNIVEVVRNGDDLQFSVGMTTASFPVDIFTVCPQGGCGVDCKDGKLRKIK 324

Query: 361 AKSLVSSA 369
           AKSLVSSA
Sbjct: 402 AKSLVSSA 324

BLAST of Spg013747 vs. NCBI nr
Match: KAF4384885.1 (hypothetical protein G4B88_000281 [Cannabis sativa])

HSP 1 Score: 310.1 bits (793), Expect = 2.6e-80
Identity = 189/465 (40.65%), Postives = 257/465 (55.27%), Query Frame = 0

Query: 9    SFLFLAFISFSSSLVSAQ--KALTAYDILQQYGFPVGILPVGVTGYELNRATGEFSLFLN 68
            S + + F S ++S  SA     L+AY +LQQYGFP G+LP GVTGYELNR TGEFS   N
Sbjct: 827  SAILIFFTSATTSFASADDYNILSAYQVLQQYGFPQGLLPKGVTGYELNRDTGEFSAHWN 886

Query: 69   QKCKFSID-SYELEYKPTINGVISQGRLRKLKGVSVKVVLLWLSIVEVVNDGDDLQFSVG 128
              CKF+++ SY LEYK TI+G+IS+ RL+ LKGVSVKV+  WL+IVEVV DGD+LQFSVG
Sbjct: 887  GTCKFNLEGSYSLEYKSTISGIISKERLKNLKGVSVKVLFFWLNIVEVVRDGDELQFSVG 946

Query: 129  IASANFPIDSFYESPQCGCGFDCNKAESLILKNMPS----------------------SW 188
            IASANFPID+F ESPQCGCG +  +     +  +P+                      S+
Sbjct: 947  IASANFPIDNFEESPQCGCGLNLLEGYDFPVGLLPNGVLGYKLDNFTGTFFAYLNGSCSF 1006

Query: 189  SQGAFELLY--------------------------WYSLDSFLR---------------- 248
            +  ++EL Y                          W  + S  R                
Sbjct: 1007 TVDSYELKYNPIIAGKITSNKLYYLSGIEVKIAFFWLKIVSVTRNNDELEFSVGFASANF 1066

Query: 249  -----ELKP----------------------------------QTHIDWRSQLAIAIAIA 308
                 E  P                                  +T I+  S  AI IA+A
Sbjct: 1067 DVSNFEESPTCGCGFDCVGYGNAAMKGTTKLSNRLVSFSSKNRKTRIETMSHTAILIAVA 1126

Query: 309  TLATFFHISMSPAAASPSASPSTSDGEAPSVYDILREFNFPIGLLPEGVMGWKLDRTTGK 368
                   +++   +   ++SP   D    S Y++L++F+ P+G+LP+GV  ++LDR+ G+
Sbjct: 1127 -------VAVVMLSQLVTSSPDVEDKR--SAYEVLQDFDLPMGILPKGVNSYELDRSNGR 1186

BLAST of Spg013747 vs. NCBI nr
Match: KAF4364443.1 (hypothetical protein F8388_007020 [Cannabis sativa])

HSP 1 Score: 310.1 bits (793), Expect = 2.6e-80
Identity = 189/465 (40.65%), Postives = 257/465 (55.27%), Query Frame = 0

Query: 9   SFLFLAFISFSSSLVSAQ--KALTAYDILQQYGFPVGILPVGVTGYELNRATGEFSLFLN 68
           S + + F S ++S  SA     L+AY +LQQYGFP G+LP GVTGYELNR TGEFS   N
Sbjct: 8   SAILIFFTSATTSFASADDYNILSAYQVLQQYGFPQGLLPKGVTGYELNRDTGEFSAHWN 67

Query: 69  QKCKFSID-SYELEYKPTINGVISQGRLRKLKGVSVKVVLLWLSIVEVVNDGDDLQFSVG 128
             CKF+++ SY LEYK TI+G+IS+ RL+ LKGVSVKV+  WL+IVEVV DGD+LQFSVG
Sbjct: 68  GTCKFNLEGSYSLEYKSTISGIISKERLKNLKGVSVKVLFFWLNIVEVVRDGDELQFSVG 127

Query: 129 IASANFPIDSFYESPQCGCGFDCNKAESLILKNMPS----------------------SW 188
           IASANFPID+F ESPQCGCG +  +     +  +P+                      S+
Sbjct: 128 IASANFPIDNFEESPQCGCGLNLLEGYDFPVGLLPNGVLGYKLDNFTGTFFAYLNGSCSF 187

Query: 189 SQGAFELLY--------------------------WYSLDSFLR---------------- 248
           +  ++EL Y                          W  + S  R                
Sbjct: 188 TVDSYELKYNPIIAGKITSNKLYYLSGIEVKIAFFWLKIVSVTRNNDELEFSVGFASANF 247

Query: 249 -----ELKP----------------------------------QTHIDWRSQLAIAIAIA 308
                E  P                                  +T I+  S  AI IA+A
Sbjct: 248 DVSNFEESPTCGCGFDCVGYGNAAMKGTTKLSNRLVSFSSKNRKTRIETMSHTAILIAVA 307

Query: 309 TLATFFHISMSPAAASPSASPSTSDGEAPSVYDILREFNFPIGLLPEGVMGWKLDRTTGK 368
                  +++   +   ++SP   D    S Y++L++F+ P+G+LP+GV  ++LDR+ G+
Sbjct: 308 -------VAVVMLSQLVTSSPDVEDKR--SAYEVLQDFDLPMGILPKGVNSYELDRSNGR 367

BLAST of Spg013747 vs. NCBI nr
Match: KAF4366655.1 (hypothetical protein G4B88_010730 [Cannabis sativa])

HSP 1 Score: 307.4 bits (786), Expect = 1.7e-79
Identity = 167/359 (46.52%), Postives = 231/359 (64.35%), Query Frame = 0

Query: 10  FLFLAFI-SFSSSLVSAQKALTAYDILQQYGFPVGILPVGVTGYELNRATGEFSLFLNQK 69
           FL L F+ SF++ L S     TAY++L+ Y FPVG+LP GV GY+L+  TG F  +LN  
Sbjct: 6   FLVLFFLMSFTTPLSSTS---TAYEVLEGYDFPVGLLPNGVLGYKLDNFTGTFFAYLNGS 65

Query: 70  CKFSIDSYELEYKPTINGVISQGRLRKLKGVSVKVVLLWLSIVEVVNDGDDLQFSVGIAS 129
           C F++DSYEL+Y P I G I+  +L  L G+ VK+   WL IV V  + D+L+FSVG AS
Sbjct: 66  CSFTVDSYELKYNPIIAGKITSNKLYYLSGIEVKIAFFWLKIVSVTRNNDELEFSVGFAS 125

Query: 130 ANFPIDSFYESPQCGCGFDCNKAESLILKNMPSSWSQGAFELLYWYSLDSFLRELKPQTH 189
           ANF + +F ESP CGCGFDC    +  +K      ++          L SF  + + +T 
Sbjct: 126 ANFDVSNFEESPTCGCGFDCVGYGNAAMKGTTKLSNR----------LVSFSSKNR-KTR 185

Query: 190 IDWRSQLAIAIAIATLATFFHISMSPAAASPSASPSTSDGEAPSVYDILREFNFPIGLLP 249
           I+  S  AI IA+A       + +S  A S   SP   D    S Y++L++F+ P+G+LP
Sbjct: 186 IETMSHTAILIAVAVAV----VMLSQLATS---SPDVEDKR--SAYEVLQDFDLPMGILP 245

Query: 250 EGVMGWKLDRTTGKLEAYLNEAFHFSPDDPYELKYKPTISGQISKNKLTDLKGVSVKFMF 309
           +GV  ++LDR+ G+  A+ N +  FS +  Y+LKYK TISG ISK+KLT L GVSVK +F
Sbjct: 246 KGVNSYELDRSNGRFYAHFNGSCSFSLEGSYQLKYKSTISGYISKDKLTKLTGVSVKVLF 305

Query: 310 FWVNIVEVVRNGDDLQFSIGMATASFPVDNFSECPQCEFGVDCKDGKVRKIKAKSLVSS 368
            W+NIVEVVR+G+DL+FS+G+A+ASF +DNF ECPQC  G++C +G+VRK+K    VSS
Sbjct: 306 LWLNIVEVVRDGNDLEFSVGLASASFSIDNFFECPQCGCGLNCNNGQVRKLKFNPSVSS 341

BLAST of Spg013747 vs. ExPASy Swiss-Prot
Match: Q9M015 (Uncharacterized protein At5g01610 OS=Arabidopsis thaliana OX=3702 GN=At5g01610 PE=1 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 2.5e-09
Identity = 28/95 (29.47%), Postives = 49/95 (51.58%), Query Frame = 0

Query: 235 DILREFNFPIGLLPEGVMGWKLDRTTGKLEAYLNEAFHFSPDDPYELKYKPTISGQISKN 294
           ++L+E++ PIG+ P     ++ D  T KL   +         D   LK+  T++G + K 
Sbjct: 56  ELLKEYDLPIGIFPGDATNYEFDEETKKLTVLIPSICEVGYKDSSVLKFTTTVTGHLEKG 115

Query: 295 KLTDLKGVSVKFMFFWVNIVEVVRNGDDLQFSIGM 330
           KLTD++G+  K M  WV +  +  +   + F+ GM
Sbjct: 116 KLTDVEGIKTKVM-IWVKVTSISTDASKVYFTAGM 149

BLAST of Spg013747 vs. ExPASy TrEMBL
Match: A0A7J6F157 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_007020 PE=4 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 1.3e-80
Identity = 189/465 (40.65%), Postives = 257/465 (55.27%), Query Frame = 0

Query: 9   SFLFLAFISFSSSLVSAQ--KALTAYDILQQYGFPVGILPVGVTGYELNRATGEFSLFLN 68
           S + + F S ++S  SA     L+AY +LQQYGFP G+LP GVTGYELNR TGEFS   N
Sbjct: 8   SAILIFFTSATTSFASADDYNILSAYQVLQQYGFPQGLLPKGVTGYELNRDTGEFSAHWN 67

Query: 69  QKCKFSID-SYELEYKPTINGVISQGRLRKLKGVSVKVVLLWLSIVEVVNDGDDLQFSVG 128
             CKF+++ SY LEYK TI+G+IS+ RL+ LKGVSVKV+  WL+IVEVV DGD+LQFSVG
Sbjct: 68  GTCKFNLEGSYSLEYKSTISGIISKERLKNLKGVSVKVLFFWLNIVEVVRDGDELQFSVG 127

Query: 129 IASANFPIDSFYESPQCGCGFDCNKAESLILKNMPS----------------------SW 188
           IASANFPID+F ESPQCGCG +  +     +  +P+                      S+
Sbjct: 128 IASANFPIDNFEESPQCGCGLNLLEGYDFPVGLLPNGVLGYKLDNFTGTFFAYLNGSCSF 187

Query: 189 SQGAFELLY--------------------------WYSLDSFLR---------------- 248
           +  ++EL Y                          W  + S  R                
Sbjct: 188 TVDSYELKYNPIIAGKITSNKLYYLSGIEVKIAFFWLKIVSVTRNNDELEFSVGFASANF 247

Query: 249 -----ELKP----------------------------------QTHIDWRSQLAIAIAIA 308
                E  P                                  +T I+  S  AI IA+A
Sbjct: 248 DVSNFEESPTCGCGFDCVGYGNAAMKGTTKLSNRLVSFSSKNRKTRIETMSHTAILIAVA 307

Query: 309 TLATFFHISMSPAAASPSASPSTSDGEAPSVYDILREFNFPIGLLPEGVMGWKLDRTTGK 368
                  +++   +   ++SP   D    S Y++L++F+ P+G+LP+GV  ++LDR+ G+
Sbjct: 308 -------VAVVMLSQLVTSSPDVEDKR--SAYEVLQDFDLPMGILPKGVNSYELDRSNGR 367

BLAST of Spg013747 vs. ExPASy TrEMBL
Match: A0A7J6GPM8 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=G4B88_000281 PE=3 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 1.3e-80
Identity = 189/465 (40.65%), Postives = 257/465 (55.27%), Query Frame = 0

Query: 9    SFLFLAFISFSSSLVSAQ--KALTAYDILQQYGFPVGILPVGVTGYELNRATGEFSLFLN 68
            S + + F S ++S  SA     L+AY +LQQYGFP G+LP GVTGYELNR TGEFS   N
Sbjct: 827  SAILIFFTSATTSFASADDYNILSAYQVLQQYGFPQGLLPKGVTGYELNRDTGEFSAHWN 886

Query: 69   QKCKFSID-SYELEYKPTINGVISQGRLRKLKGVSVKVVLLWLSIVEVVNDGDDLQFSVG 128
              CKF+++ SY LEYK TI+G+IS+ RL+ LKGVSVKV+  WL+IVEVV DGD+LQFSVG
Sbjct: 887  GTCKFNLEGSYSLEYKSTISGIISKERLKNLKGVSVKVLFFWLNIVEVVRDGDELQFSVG 946

Query: 129  IASANFPIDSFYESPQCGCGFDCNKAESLILKNMPS----------------------SW 188
            IASANFPID+F ESPQCGCG +  +     +  +P+                      S+
Sbjct: 947  IASANFPIDNFEESPQCGCGLNLLEGYDFPVGLLPNGVLGYKLDNFTGTFFAYLNGSCSF 1006

Query: 189  SQGAFELLY--------------------------WYSLDSFLR---------------- 248
            +  ++EL Y                          W  + S  R                
Sbjct: 1007 TVDSYELKYNPIIAGKITSNKLYYLSGIEVKIAFFWLKIVSVTRNNDELEFSVGFASANF 1066

Query: 249  -----ELKP----------------------------------QTHIDWRSQLAIAIAIA 308
                 E  P                                  +T I+  S  AI IA+A
Sbjct: 1067 DVSNFEESPTCGCGFDCVGYGNAAMKGTTKLSNRLVSFSSKNRKTRIETMSHTAILIAVA 1126

Query: 309  TLATFFHISMSPAAASPSASPSTSDGEAPSVYDILREFNFPIGLLPEGVMGWKLDRTTGK 368
                   +++   +   ++SP   D    S Y++L++F+ P+G+LP+GV  ++LDR+ G+
Sbjct: 1127 -------VAVVMLSQLVTSSPDVEDKR--SAYEVLQDFDLPMGILPKGVNSYELDRSNGR 1186

BLAST of Spg013747 vs. ExPASy TrEMBL
Match: A0A7J6F7M6 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=G4B88_010730 PE=4 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 8.2e-80
Identity = 167/359 (46.52%), Postives = 231/359 (64.35%), Query Frame = 0

Query: 10  FLFLAFI-SFSSSLVSAQKALTAYDILQQYGFPVGILPVGVTGYELNRATGEFSLFLNQK 69
           FL L F+ SF++ L S     TAY++L+ Y FPVG+LP GV GY+L+  TG F  +LN  
Sbjct: 6   FLVLFFLMSFTTPLSSTS---TAYEVLEGYDFPVGLLPNGVLGYKLDNFTGTFFAYLNGS 65

Query: 70  CKFSIDSYELEYKPTINGVISQGRLRKLKGVSVKVVLLWLSIVEVVNDGDDLQFSVGIAS 129
           C F++DSYEL+Y P I G I+  +L  L G+ VK+   WL IV V  + D+L+FSVG AS
Sbjct: 66  CSFTVDSYELKYNPIIAGKITSNKLYYLSGIEVKIAFFWLKIVSVTRNNDELEFSVGFAS 125

Query: 130 ANFPIDSFYESPQCGCGFDCNKAESLILKNMPSSWSQGAFELLYWYSLDSFLRELKPQTH 189
           ANF + +F ESP CGCGFDC    +  +K      ++          L SF  + + +T 
Sbjct: 126 ANFDVSNFEESPTCGCGFDCVGYGNAAMKGTTKLSNR----------LVSFSSKNR-KTR 185

Query: 190 IDWRSQLAIAIAIATLATFFHISMSPAAASPSASPSTSDGEAPSVYDILREFNFPIGLLP 249
           I+  S  AI IA+A       + +S  A S   SP   D    S Y++L++F+ P+G+LP
Sbjct: 186 IETMSHTAILIAVAVAV----VMLSQLATS---SPDVEDKR--SAYEVLQDFDLPMGILP 245

Query: 250 EGVMGWKLDRTTGKLEAYLNEAFHFSPDDPYELKYKPTISGQISKNKLTDLKGVSVKFMF 309
           +GV  ++LDR+ G+  A+ N +  FS +  Y+LKYK TISG ISK+KLT L GVSVK +F
Sbjct: 246 KGVNSYELDRSNGRFYAHFNGSCSFSLEGSYQLKYKSTISGYISKDKLTKLTGVSVKVLF 305

Query: 310 FWVNIVEVVRNGDDLQFSIGMATASFPVDNFSECPQCEFGVDCKDGKVRKIKAKSLVSS 368
            W+NIVEVVR+G+DL+FS+G+A+ASF +DNF ECPQC  G++C +G+VRK+K    VSS
Sbjct: 306 LWLNIVEVVRDGNDLEFSVGLASASFSIDNFFECPQCGCGLNCNNGQVRKLKFNPSVSS 341

BLAST of Spg013747 vs. ExPASy TrEMBL
Match: A0A5E4FHY3 (PREDICTED: At5g01610 OS=Prunus dulcis OX=3755 GN=ALMOND_2B007890 PE=4 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 5.3e-79
Identity = 160/336 (47.62%), Postives = 221/336 (65.77%), Query Frame = 0

Query: 34  ILQQYGFPVGILPVGVTGYELNRATGEFSLFLNQKCKFSID-SYELEYKPTINGVISQGR 93
           +LQQY FP+G+LP GVTGYEL+R TG F  + N  C FS++ SY+L Y+ TI GV+S+ R
Sbjct: 1   MLQQYDFPIGLLPKGVTGYELDRDTGNFKAYFNGTCSFSLENSYQLRYRSTITGVLSKDR 60

Query: 94  LRKLKGVSVKVVLLWLSIVEVVNDGDDLQFSVGIASANFPIDSFYESPQCGCGFDCNKAE 153
           L+ LKGVSVKV+  W+ IVEVV +GD+LQFSVGIASA+F ID+F ESPQCGCGF C   +
Sbjct: 61  LKNLKGVSVKVLFFWIDIVEVVRNGDELQFSVGIASADFSIDNFEESPQCGCGFAC---Q 120

Query: 154 SLILKNMPSSWSQGAFELLYWYSLDSFLRELKPQTHIDWRSQLAIAIAIATLATFFHISM 213
            L+   +PS  S                  L  QT +   S ++    +  L+ F  +S 
Sbjct: 121 QLVSSAVPSHSS------------------LHNQT-VKTSSTMSPKETLLWLSLFLLVSF 180

Query: 214 SPAAASPSASPSTSDGEAPSVYDILREFNFPIGLLPEGVMGWKLDRTTGKLEAYLNEAFH 273
           S        +PS S  +  +VY++L +++FP+G+LP+G +G++LD +TGK   YLN    
Sbjct: 181 S--------TPSASVNDELTVYEVLEDYDFPVGILPKGALGYELDNSTGKFTVYLNGTCS 240

Query: 274 FSPDDPYELKYKPTISGQISKNKLTDLKGVSVKFMFFWVNIVEVVRNGDDLQFSIGMATA 333
           FS D  Y+LKYK TI+G I+ +K++ L G+ VK +F W++IV+V+R+ D L+FS+G+A+A
Sbjct: 241 FSIDS-YKLKYKSTITGVITDDKISSLSGIKVKVLFLWLSIVKVIRDDDKLEFSVGIASA 300

Query: 334 SFPVDNFSECPQCEFGVDCKDGKVRKIKAKSLVSSA 369
            FPV NF E P C  G DC +GK RKIK  +LVSSA
Sbjct: 301 DFPVSNFVESPTCGCGFDCVNGKGRKIKINNLVSSA 305

BLAST of Spg013747 vs. ExPASy TrEMBL
Match: A0A371H333 (Uncharacterized protein (Fragment) OS=Mucuna pruriens OX=157652 GN=CR513_20056 PE=4 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 5.9e-78
Identity = 162/360 (45.00%), Postives = 226/360 (62.78%), Query Frame = 0

Query: 4   SKLMASFLFLAFISFSSSLVSAQKALTAYDILQQYGFPVGILPVGVTGYELNRATGEFSL 63
           S +   ++ +  ++ S +L   QK L+AYD+L +YGFPVG+LP G  GY LNR TGEF++
Sbjct: 2   SSIRLVWILVVCLTASCALSQPQK-LSAYDVLVEYGFPVGLLPKGAIGYSLNRDTGEFAV 61

Query: 64  FLNQKCKFSIDSYELEYKPTINGVISQGRLRKLKGVSVKVVLLWLSIVEVVNDGDDLQFS 123
           + ++ C F I+SY L YK TI GVIS+ RL KLKGV+VKV+LLWL+IVEV    DD+ FS
Sbjct: 62  YFDKSCSFDIESYSLNYKSTITGVISKTRLYKLKGVTVKVLLLWLNIVEVTRKADDILFS 121

Query: 124 VGIASANFPIDSFYESPQCGCGFDCNKAESLILKNMPSSWSQGAFELLYWYSLDSFLREL 183
           VGIASA+F +++F ESPQCGCGFDCNK     L     S S  A  LL   S        
Sbjct: 122 VGIASADFGVENFLESPQCGCGFDCNK-----LPLNGDSMSMSALLLLLLVS-------- 181

Query: 184 KPQTHIDWRSQLAIAIAIATLATFFHISMSPAAASPSASPSTSDGEAPSVYDILREFNFP 243
                                        +P AAS           A + Y++L  F+FP
Sbjct: 182 -----------------------------APIAAS----------GALTAYEMLESFHFP 241

Query: 244 IGLLPEGVMGWKLDRTTGKLEAYLNEAFHFSPDDPYELKYKPTISGQISKNKLTDLKGVS 303
            G+LP+GV G++L+R++GK  A LN +  F+ +  Y+L Y+PTI+G IS  +LT+L+G+S
Sbjct: 242 EGILPKGVTGYELERSSGKFRADLNGSCSFALEGSYKLSYQPTITGYISDGRLTELRGIS 301

Query: 304 VKFMFFWVNIVEVVRNGDDLQFSIGMATASFPVDNFSECPQCEFGVDCKDGKVRKIKAKS 363
           VK +FFW+NI++V R GDDL FS+G+A+ASFP++NF   PQC  G+DC D ++R++K ++
Sbjct: 302 VKVLFFWLNIIDVTRVGDDLYFSVGVASASFPLNNFFVSPQCGCGLDCDDFRIRQLKFRN 308

BLAST of Spg013747 vs. TAIR 10
Match: AT1G02816.1 (Protein of unknown function, DUF538 )

HSP 1 Score: 165.6 bits (418), Expect = 7.4e-41
Identity = 81/157 (51.59%), Postives = 107/157 (68.15%), Query Frame = 0

Query: 6   LMASFLFLAFISFSSSLVSA---QKALTAYDILQQYGFPVGILPVGVTGYELNRATGEFS 65
           L  S  F       S L++A       TAY +LQ Y FPVGILP GV  Y+L+++TG+F 
Sbjct: 4   LPISIFFFFLFCIPSPLIAAANDDDIPTAYTLLQSYNFPVGILPKGVVSYDLDKSTGQFH 63

Query: 66  LFLNQKCKFSID-SYELEYKPTINGVISQGRLRKLKGVSVKVVLLWLSIVEVVNDGDDLQ 125
            + N+ C F++  SY+L+YK TI+G IS+ ++ KL GV VKV+ LWL+IVEV+ +GD+L+
Sbjct: 64  AYFNKSCSFALQGSYQLDYKSTISGYISENKITKLTGVKVKVLFLWLNIVEVIRNGDELE 123

Query: 126 FSVGIASANFPIDSFYESPQCGCGFDCNKAESLILKN 159
           FSVGI SANF ID FYESPQCGCGFDC      + +N
Sbjct: 124 FSVGITSANFEIDEFYESPQCGCGFDCKTKTKTLGRN 160

BLAST of Spg013747 vs. TAIR 10
Match: AT4G02370.1 (Protein of unknown function, DUF538 )

HSP 1 Score: 164.5 bits (415), Expect = 1.7e-40
Identity = 78/156 (50.00%), Postives = 110/156 (70.51%), Query Frame = 0

Query: 6   LMASFLFLAFISFSSSLVSAQKALTAYDILQQYGFPVGILPVGVTGYELNRATGEFSLFL 65
           L+AS LFL+ ++ +    +     TAY +LQ Y FPVGILP GV  Y+L+  TG+F  + 
Sbjct: 7   LIASCLFLSSLTAAVVTAAESDTPTAYSLLQSYNFPVGILPKGVVAYDLDTTTGKFHAYF 66

Query: 66  NQKCKFS-IDSYELEYKPTINGVISQGRLRKLKGVSVKVVLLWLSIVEVVNDGDDLQFSV 125
           N  C F+ + SY+L YK TI+G IS+ +L+KL GV VKV+ LWL+IVEV+ +GD+++FSV
Sbjct: 67  NDSCSFNLVGSYQLNYKSTISGYISENKLKKLTGVKVKVLFLWLNIVEVIRNGDEMEFSV 126

Query: 126 GIASANFPIDSFYESPQCGCGFDCNKAESLILKNMP 161
           GI SANF I  F ESPQCGCGF+C  ++  +++ +P
Sbjct: 127 GITSANFAIQEFLESPQCGCGFECKDSKLDMIERIP 162

BLAST of Spg013747 vs. TAIR 10
Match: AT4G02360.1 (Protein of unknown function, DUF538 )

HSP 1 Score: 161.4 bits (407), Expect = 1.4e-39
Identity = 84/149 (56.38%), Postives = 103/149 (69.13%), Query Frame = 0

Query: 1   MSFSKLMASFLFLAFISFSSSLVSAQKALTAYDILQQYGFPVGILPVGVTGYELNRATGE 60
           MS      S +F  F + S + VS QK  TAYD ++ Y  P GILP GV  YELN  TG 
Sbjct: 1   MSLDANTCSIVFFLFFTVSFA-VSGQKP-TAYDAVKLYNLPPGILPKGVVDYELNPKTGN 60

Query: 61  FSLFLNQKCKFSIDSYELEYKPTINGVISQGRLRKLKGVSVKVVLLWLSIVEVVNDGDDL 120
           F ++ N  C+F+I SY+L+YK TI+GVIS G ++ LKGVSVKV+  W++I EV  DG DL
Sbjct: 61  FKVYFNDTCEFTIQSYQLKYKSTISGVISPGHVKNLKGVSVKVLFFWVNIAEVSLDGADL 120

Query: 121 QFSVGIASANFPIDSFYESPQCGCGFDCN 150
            FSVGIASA+FP  +F ESPQCGCGFDCN
Sbjct: 121 DFSVGIASASFPAANFEESPQCGCGFDCN 147

BLAST of Spg013747 vs. TAIR 10
Match: AT1G02813.1 (Protein of unknown function, DUF538 )

HSP 1 Score: 146.4 bits (368), Expect = 4.6e-35
Identity = 70/142 (49.30%), Postives = 97/142 (68.31%), Query Frame = 0

Query: 7   MASFLFLAFISFSSSLVSAQKALTAYDILQQYGFPVGILPVGVTGYELNRATGEFSLFLN 66
           M+ F+    +  +S  VS QK  + Y +L+ Y  P GILP GV  Y+LNR TG F +  N
Sbjct: 1   MSIFIIFLLLLSTSVSVSGQKKRSVYQVLENYTLPRGILPEGVHDYDLNRRTGVFKVRFN 60

Query: 67  QKCKFSIDSYELEYKPTINGVISQGRLRKLKGVSVKVVLLWLSIVEVVNDGDDLQFSVGI 126
             C+FSIDSY+++YKP I+G+I++GR+ +L GVSVKV+  W++I EV  DGDD++F VG 
Sbjct: 61  TTCQFSIDSYKVKYKPVISGIITRGRVIRLIGVSVKVLFFWINISEVSRDGDDVEFFVGA 120

Query: 127 ASANFPIDSFYESPQCGCGFDC 149
           AS  F    F +SP+CGCGF+C
Sbjct: 121 ASEEFSSKYFVDSPKCGCGFNC 142

BLAST of Spg013747 vs. TAIR 10
Match: AT5G19590.1 (Protein of unknown function, DUF538 )

HSP 1 Score: 92.4 bits (228), Expect = 8.0e-19
Identity = 53/144 (36.81%), Postives = 79/144 (54.86%), Query Frame = 0

Query: 1   MSFSKLMASFLFLAFISFSSSLVSAQKALTAYDILQQYGFPVGILPVGVTGYELNRATGE 60
           M  S  + S L L  IS        +K   A+  L  +GFP+G+LP+ V  Y LN+ +G+
Sbjct: 1   MKISLFLLSILILLPISLQDPTPEVKKPTRAHAELTNHGFPIGLLPLSVKDYFLNQTSGD 60

Query: 61  FSLFLNQKCKFSI--DSYELEYKPTINGVISQGRLRKLKGVSVKVVLLWLSIVEVVNDGD 120
           FSLFLN  CK ++  D+Y   Y   + G ISQG++ +L+G+ V+      SI  + + GD
Sbjct: 61  FSLFLNGACKITLPPDNYIATYSNKVTGRISQGKIAELQGIRVRAFFKSWSITGIRSSGD 120

Query: 121 DLQFSVGIASANFPIDSFYESPQC 143
           +L F V   +A +P  +F ES  C
Sbjct: 121 NLVFEVAGITAKYPSKNFDESLDC 144