Spg013703 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg013703
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionmitogen-activated protein kinase kinase kinase 17-like
Locationscaffold3: 45318666 .. 45319598 (+)
RNA-Seq ExpressionSpg013703
SyntenySpg013703
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGAACTGGGTTCGACTGAAGATCTTAGGCGAAGGTTCTTACGGCGTAGTATTCTTAGCAAAACCAACAACCGGAACCCTCGACAACTTCTTCGCCATCAAAGCAACTCACCTTCAACACTATTCTTCACTCTCCAAAGAAAAACAGATTCTAAAACATTTCATCGGATGTCCCGAAATCGTTCAATATTTAGGGAGCGAAATAACCATAGAAAAATACTCCCCGGACATGGCCTTTTACAATCTGAAACTGGAGTACGCCGCCGGCGGAACTCTAGCCGACCTGATCAAACAGAAGCAGAAGCTGCCGGAGAACGAAGTGAAGCAGTACCTCAAAATGATCCTCAGAGGACTCTCCTGCATTCATCGCAAAGGGTTCGTCCACGTCGATCTCAAGCCCAGCAACATCCTCGCATTCCCTCAAAGCGACGGCAAGACGAAGCTGAAGATCGCCGACTTCGGGCTTTCGAGGAGACGCGGCGAGGACGACGGTGAGGATTATCGCGGAGTTCCGAGGTTTAAAGGGACGCCGAAATACATGTCGCCGGAATCGGTTATTTTCAGTGAGGTGAAGAGTTCTCTGGATATCTGGTCGCTGGGCTGCATTTTGGTTGAAATGGTTTCGGGAAGATCGGTGTGGGAGGATTGTCGGAGTCGCAGAGAGTTTGTTTCGAAACTTGTGTGTAGCGAGGAAATGCCGGAGATTCCTGAGGAATTGTCGGAAGAAGGGAAGGATTTCATTGGAAGATGCTTTGATCGGGACCATGAACGGCGGTGGACGGCGGATATGTTACTCCGGCATCCGTATGTTAGAGAAGAAAAGGAGGAAGGGATGACAGCTAAACTTCCGGGAAGACCAAAATTTCCGAAAGGGATTTATTTGCCTCATCTACAATGCAAGCTTCGATCGTGTATTCAAATTGCTTCATAA

mRNA sequence

ATGACGAACTGGGTTCGACTGAAGATCTTAGGCGAAGGTTCTTACGGCGTAGTATTCTTAGCAAAACCAACAACCGGAACCCTCGACAACTTCTTCGCCATCAAAGCAACTCACCTTCAACACTATTCTTCACTCTCCAAAGAAAAACAGATTCTAAAACATTTCATCGGATGTCCCGAAATCGTTCAATATTTAGGGAGCGAAATAACCATAGAAAAATACTCCCCGGACATGGCCTTTTACAATCTGAAACTGGAGTACGCCGCCGGCGGAACTCTAGCCGACCTGATCAAACAGAAGCAGAAGCTGCCGGAGAACGAAGTGAAGCAGTACCTCAAAATGATCCTCAGAGGACTCTCCTGCATTCATCGCAAAGGGTTCGTCCACGTCGATCTCAAGCCCAGCAACATCCTCGCATTCCCTCAAAGCGACGGCAAGACGAAGCTGAAGATCGCCGACTTCGGGCTTTCGAGGAGACGCGGCGAGGACGACGGTGAGGATTATCGCGGAGTTCCGAGGTTTAAAGGGACGCCGAAATACATGTCGCCGGAATCGGTTATTTTCAGTGAGGTGAAGAGTTCTCTGGATATCTGGTCGCTGGGCTGCATTTTGGTTGAAATGGTTTCGGGAAGATCGGTGTGGGAGGATTGTCGGAGTCGCAGAGAGTTTGTTTCGAAACTTGTGTGTAGCGAGGAAATGCCGGAGATTCCTGAGGAATTGTCGGAAGAAGGGAAGGATTTCATTGGAAGATGCTTTGATCGGGACCATGAACGGCGGTGGACGGCGGATATGTTACTCCGGCATCCGTATGTTAGAGAAGAAAAGGAGGAAGGGATGACAGCTAAACTTCCGGGAAGACCAAAATTTCCGAAAGGGATTTATTTGCCTCATCTACAATGCAAGCTTCGATCGTGTATTCAAATTGCTTCATAA

Coding sequence (CDS)

ATGACGAACTGGGTTCGACTGAAGATCTTAGGCGAAGGTTCTTACGGCGTAGTATTCTTAGCAAAACCAACAACCGGAACCCTCGACAACTTCTTCGCCATCAAAGCAACTCACCTTCAACACTATTCTTCACTCTCCAAAGAAAAACAGATTCTAAAACATTTCATCGGATGTCCCGAAATCGTTCAATATTTAGGGAGCGAAATAACCATAGAAAAATACTCCCCGGACATGGCCTTTTACAATCTGAAACTGGAGTACGCCGCCGGCGGAACTCTAGCCGACCTGATCAAACAGAAGCAGAAGCTGCCGGAGAACGAAGTGAAGCAGTACCTCAAAATGATCCTCAGAGGACTCTCCTGCATTCATCGCAAAGGGTTCGTCCACGTCGATCTCAAGCCCAGCAACATCCTCGCATTCCCTCAAAGCGACGGCAAGACGAAGCTGAAGATCGCCGACTTCGGGCTTTCGAGGAGACGCGGCGAGGACGACGGTGAGGATTATCGCGGAGTTCCGAGGTTTAAAGGGACGCCGAAATACATGTCGCCGGAATCGGTTATTTTCAGTGAGGTGAAGAGTTCTCTGGATATCTGGTCGCTGGGCTGCATTTTGGTTGAAATGGTTTCGGGAAGATCGGTGTGGGAGGATTGTCGGAGTCGCAGAGAGTTTGTTTCGAAACTTGTGTGTAGCGAGGAAATGCCGGAGATTCCTGAGGAATTGTCGGAAGAAGGGAAGGATTTCATTGGAAGATGCTTTGATCGGGACCATGAACGGCGGTGGACGGCGGATATGTTACTCCGGCATCCGTATGTTAGAGAAGAAAAGGAGGAAGGGATGACAGCTAAACTTCCGGGAAGACCAAAATTTCCGAAAGGGATTTATTTGCCTCATCTACAATGCAAGCTTCGATCGTGTATTCAAATTGCTTCATAA

Protein sequence

MTNWVRLKILGEGSYGVVFLAKPTTGTLDNFFAIKATHLQHYSSLSKEKQILKHFIGCPEIVQYLGSEITIEKYSPDMAFYNLKLEYAAGGTLADLIKQKQKLPENEVKQYLKMILRGLSCIHRKGFVHVDLKPSNILAFPQSDGKTKLKIADFGLSRRRGEDDGEDYRGVPRFKGTPKYMSPESVIFSEVKSSLDIWSLGCILVEMVSGRSVWEDCRSRREFVSKLVCSEEMPEIPEELSEEGKDFIGRCFDRDHERRWTADMLLRHPYVREEKEEGMTAKLPGRPKFPKGIYLPHLQCKLRSCIQIAS
Homology
BLAST of Spg013703 vs. NCBI nr
Match: XP_022150204.1 (mitogen-activated protein kinase kinase kinase 17-like [Momordica charantia])

HSP 1 Score: 371.3 bits (952), Expect = 8.0e-99
Identity = 192/308 (62.34%), Postives = 234/308 (75.97%), Query Frame = 0

Query: 1   MTNWVRLKILGEGSYGVVFLAKPTTGTLDNFFAIKATHLQHYSSLSKEKQILKHFIGCPE 60
           MTNWVR+  LG+GSYG VFLAKPTT +L  +FA+K   LQHYSSL  EK+ILKHF+G PE
Sbjct: 1   MTNWVRVXDLGKGSYGKVFLAKPTTRSL-KYFAVKVAELQHYSSLLNEKRILKHFVGFPE 60

Query: 61  IVQYLGSEITIEKYSPDMAFYNLKLEYAAGGTLADLIKQKQKLPENEVKQYLKMILRGLS 120
           IVQ  G E+  E  S D AFY+L LEYAAGGTLADLI++++KL EN VK+YLKMILRGLS
Sbjct: 61  IVQCFGGEMITEGDSFDSAFYSLVLEYAAGGTLADLIRKQKKLSENTVKEYLKMILRGLS 120

Query: 121 CIHRKGFVHVDLKPSNILAFPQSDGKTKLKIADFGLSRRRGEDDGEDYRGVPRFKGTPKY 180
           CIH+ GFVHVDLKP+NILAFPQ DGK KLKIADFGLSRR  +    +++   +F+GTP Y
Sbjct: 121 CIHKLGFVHVDLKPANILAFPQIDGKMKLKIADFGLSRRCNKKRECNFK--LKFRGTPMY 180

Query: 181 MSPESVIFSEVKSSLDIWSLGCILVEMVSGRSVWEDCRSRREFVSKLVCSEEMPEIPEEL 240
           MSPES I+ EV + LDIWSLGCILVEMV+G+  W+DC    + + KL  + E P+IPEEL
Sbjct: 181 MSPES-IYGEVNTPLDIWSLGCILVEMVTGKHAWDDCIDLEQLMLKLRVNMETPKIPEEL 240

Query: 241 SEEGKDFIGRCFDRDHERRWTADMLLRHPYVREEKEEGMTAKLPGRPKFPKGIYLPHLQC 300
           S+EGKDF+ +CFDR+ +RRWTADMLL+HPY+ +EKE     K    P +    YLPH+  
Sbjct: 241 SKEGKDFLEKCFDRNPKRRWTADMLLQHPYLEQEKE---AIKKDDDPTYS---YLPHILY 298

Query: 301 KLRSCIQI 309
           K +S IQI
Sbjct: 301 KFQSTIQI 298

BLAST of Spg013703 vs. NCBI nr
Match: XP_038896325.1 (mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida])

HSP 1 Score: 369.0 bits (946), Expect = 4.0e-98
Identity = 186/301 (61.79%), Postives = 228/301 (75.75%), Query Frame = 0

Query: 1   MTNWVRLKILGEGSYGVVFLAKPTTGTLDN---FFAIKATHLQHYSSLSKEKQILKHFIG 60
           M NWV +KI+G GSYGVV LAK  T    +   +FA+K T LQ  SSL  E+Q+LKHF  
Sbjct: 1   MENWVLVKIIGRGSYGVVCLAKQMTKEKSDRHYYFAVKLTSLQRKSSLLWEEQVLKHFKD 60

Query: 61  CPEIVQYLGSEITIEKYSPDMAFYNLKLEYAAGGTLADLIKQKQKLPENEVKQYLKMILR 120
           CPEIVQ LGSEIT      DM  YNLKLEYA GGTLADLIKQ++KLPE+EVK YL+MIL+
Sbjct: 61  CPEIVQCLGSEITFGDIPDDMVLYNLKLEYAPGGTLADLIKQRKKLPEDEVKDYLRMILK 120

Query: 121 GLSCIHRKGFVHVDLKPSNILAFPQSDGKTKLKIADFGLSRR-RGEDDGEDYRGVPRFKG 180
           GLSCIHR+GFVHVDLKP+N+L FPQSDGK KLKIADFGL+ R +   D +D   + +FKG
Sbjct: 121 GLSCIHRRGFVHVDLKPANVLVFPQSDGKMKLKIADFGLAERCKHIVDDQDRGYLRKFKG 180

Query: 181 TPKYMSPESVIFSEVKSSLDIWSLGCILVEMVSGRSVWEDCRSRREFVSKLVCSEEMPEI 240
           TPKYMSPES+ F+EV  + DIWSLGCILV+MVSG+SVW+DCR   + +++L+  + +P I
Sbjct: 181 TPKYMSPESIFFNEVNGTHDIWSLGCILVKMVSGKSVWDDCRGSEQLMTRLLNDKNIPTI 240

Query: 241 PEELSEEGKDFIGRCFDRDHERRWTADMLLRHPYVREEKEEGMTAKLPGRPKFPKGIYLP 298
           P+ELS++GKDFI RCF R++++RWTADMLL+HPY+ EE E   T K     K  K I LP
Sbjct: 241 PKELSKQGKDFIKRCFIRNYKQRWTADMLLQHPYLNEENE--ATKKGDDIVKLIKAIRLP 299

BLAST of Spg013703 vs. NCBI nr
Match: XP_004136151.1 (mitogen-activated protein kinase kinase kinase 17 [Cucumis sativus] >KGN45019.1 hypothetical protein Csa_015671 [Cucumis sativus])

HSP 1 Score: 360.1 bits (923), Expect = 1.9e-95
Identity = 188/309 (60.84%), Postives = 230/309 (74.43%), Query Frame = 0

Query: 3   NWVRLKILGEGSYGVVFLAKPTT---GTLDNFFAIKATHLQH-YSSLSKEKQILKHFIGC 62
           +WV +K LGEGS G+V LAK  T     L  +FA+K   L++  SSL  E+ +LKHF  C
Sbjct: 4   DWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLWEEHVLKHFTDC 63

Query: 63  PEIVQYLGSEIT-IEKYSPDMAFYNLKLEYAAGGTLADLIKQKQKLPENEVKQYLKMILR 122
           PEIVQYLGSE+T    +  D   YNLKLEYAAGGTLADLIKQ+ KLPE+EVK+YL+MIL+
Sbjct: 64  PEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQMILK 123

Query: 123 GLSCIHRKGFVHVDLKPSNILAFPQSDGKTKLKIADFGLSRR---RGEDDGED-----YR 182
           GLSCIHRKGFVHVDLKP NILAFPQSDGK KLKIADFGL+ R   RGEDD ED     Y 
Sbjct: 124 GLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGSKYYS 183

Query: 183 GVPRFKGTPKYMSPESVIFSEVKSSLDIWSLGCILVEMVSGRSVWEDCRSRREFVSKLVC 242
           G  + + T +YMSPES++FSE+    DIWSLGC LV+MVSG  VW DC+S  E ++KL+ 
Sbjct: 184 GALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCKSYEELITKLLI 243

Query: 243 SEEMPEIPEELSEEGKDFIGRCFDRDHERRWTADMLLRHPYVREEKEEGMTAKLPGRPKF 298
           SEE+P IPEELS++GKDF+ +CF R++E+RWTADMLL+HPY+ EE ++  T     + K 
Sbjct: 244 SEEIPTIPEELSKQGKDFLEKCFVRNYEQRWTADMLLQHPYLNEENKD--TKNGDEKLKL 303

BLAST of Spg013703 vs. NCBI nr
Match: KAA0046699.1 (serine/threonine-protein kinase Chk1-like [Cucumis melo var. makuwa] >TYK18235.1 serine/threonine-protein kinase Chk1-like [Cucumis melo var. makuwa])

HSP 1 Score: 347.8 bits (891), Expect = 9.5e-92
Identity = 179/308 (58.12%), Postives = 227/308 (73.70%), Query Frame = 0

Query: 1   MTNWVRLKILGEGSYGVVFLAKPTT---GTLDNFFAIKATHLQHYS-SLSKEKQILKHFI 60
           M +WV LK+LGEGS G+V LAK  T     L  +FA+K   L++ S SL  E+ +LKH  
Sbjct: 1   MEDWVLLKVLGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSASLLWEEHVLKHLT 60

Query: 61  GCPEIVQYLGSEIT-IEKYSPDMAFYNLKLEYAAGGTLADLIKQKQKLPENEVKQYLKMI 120
            CPEIVQYLGSE+T    +  D   YNLKLEYAAGGTLADLIKQ+ KLPE+EVK+YL+MI
Sbjct: 61  DCPEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQMI 120

Query: 121 LRGLSCIHRKGFVHVDLKPSNILAFPQSDGKTKLKIADFGLSRR--RGEDDGED----YR 180
           L+GLSCIHRKGFVHVDLKP NILAFPQSDGK KLKIADFGL+ R   GEDD ED    + 
Sbjct: 121 LKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERFKHGEDDQEDKGCEHY 180

Query: 181 GVPRFKGTPKYMSPESVIFSEVKSSLDIWSLGCILVEMVSGRSVWEDCRSRREFVSKLVC 240
           G  R + T +YMSPES++  E+    DIWSLGC+LV+MVSG  VW DC++  E ++KL+ 
Sbjct: 181 GALRVRATHRYMSPESIVLKEIDGLHDIWSLGCVLVQMVSGERVWNDCKNYEELMTKLLN 240

Query: 241 SEEMPEIPEELSEEGKDFIGRCFDRDHERRWTADMLLRHPYVREEKEEGMTAKLPGRPKF 298
           S+E+P IP+ELS++G DF+ +CF RD+++RWTADMLL+HPY+ E+ ++    +   + K 
Sbjct: 241 SKEIPIIPKELSKQGIDFLEKCFVRDYKQRWTADMLLQHPYLNEQNKDTSDDE---KLKL 300

BLAST of Spg013703 vs. NCBI nr
Match: KAA0059705.1 (mitogen-activated protein kinase kinase kinase ANP1-like [Cucumis melo var. makuwa] >TYK26143.1 mitogen-activated protein kinase kinase kinase ANP1-like [Cucumis melo var. makuwa])

HSP 1 Score: 347.4 bits (890), Expect = 1.2e-91
Identity = 175/297 (58.92%), Postives = 221/297 (74.41%), Query Frame = 0

Query: 1   MTNWVRLKILGEGSYGVVFLAKPTT-------GTLDNFFAIKATHLQHYSSLSKEKQILK 60
           M NWV +K+LG+GSYGVV LAK ++         L  +FA+K + LQH SSL  E+Q+LK
Sbjct: 1   MENWVPVKVLGQGSYGVVCLAKQSSAIRKKSDSNLPYYFAVKLSPLQHNSSLLWEEQVLK 60

Query: 61  HFIGCPEIVQYLGSEITIEK--YSPDMAFYNLKLEYAAGGTLADLIKQKQKLPENEVKQY 120
            F  CPEIVQYLGSEIT  +   +  + FY LKLEYAAGGTL DLI+Q+ KLPE EVK Y
Sbjct: 61  QFKDCPEIVQYLGSEITRGRDIRNDHVDFYTLKLEYAAGGTLDDLIEQRDKLPEAEVKDY 120

Query: 121 LKMILRGLSCIHRKGFVHVDLKPSNILAFPQSDGKTKLKIADFGLSRRRGEDDGEDYRGV 180
           L+MIL+GLSCIH KGFVHVDLKP+NILAFPQSDGK KLKIADFG + R      + ++  
Sbjct: 121 LRMILKGLSCIHSKGFVHVDLKPNNILAFPQSDGKMKLKIADFGQAERSKYRVDDQHKQY 180

Query: 181 P------RFKGTPKYMSPESVIFSEVKSSLDIWSLGCILVEMVSGRSVWEDCRSRREFVS 240
           P      +FKGTP+YMSPESV+F+EV  + DIWSLGCILV+M+SG+S W+DC+  ++ + 
Sbjct: 181 PPCCSSFKFKGTPRYMSPESVVFNEVNDADDIWSLGCILVKMISGKSAWDDCKDSKQLMI 240

Query: 241 KLVCSEEMPEIPEELSEEGKDFIGRCFDRDHERRWTADMLLRHPYVREEKEEGMTAK 283
           KL+ ++ MP IP ELSE+GKDFI +CF R+++ RWTA+MLL+HPY+ EE E  M  K
Sbjct: 241 KLLDNKIMPTIPGELSEQGKDFIKKCFIRNYKERWTAEMLLQHPYLNEENEAPMNNK 297

BLAST of Spg013703 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 7.2e-50
Identity = 116/281 (41.28%), Postives = 170/281 (60.50%), Query Frame = 0

Query: 4   WVRLKILGEGSYGVVFLAKPT--TGTLDNFFAIKATHLQHYSSLSKEKQILKHFIGCPEI 63
           WVR + +G G++  V  A  +  +G      A+K+T     +SLS EK +L     CPEI
Sbjct: 3   WVRGETIGFGTFSTVSTATKSRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDCPEI 62

Query: 64  VQYLGSEITIEKYSPDMAFYNLKLEYAAGGTLADLIKQ--KQKLPENEVKQYLKMILRGL 123
           ++  G + T+E        +NL LEYA+ G+LA  +K+   + LPE+ V+++   +LRGL
Sbjct: 63  IRCYGEDSTVEN---GEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGL 122

Query: 124 SCIHRKGFVHVDLKPSNILAFPQSDGKTKLKIADFGLSRRRGEDDGEDYRGVPRFKGTPK 183
             IH KGF H D+K +NIL F  +DG   +KIADFGL+  R + D    R     +GTP 
Sbjct: 123 RHIHAKGFAHCDIKLANILLF--NDG--SVKIADFGLA-MRVDGDLTALRKSVEIRGTPL 182

Query: 184 YMSPESVIFSEVKSSLDIWSLGCILVEMVSGRSVWEDCRSRREFVSKLV---CSEEMPEI 243
           YM+PE V  +E  S+ D+W+LGC +VEM SG++ W   +    F+S L+     +E+P+I
Sbjct: 183 YMAPECVNDNEYGSAADVWALGCAVVEMFSGKTAW-SVKEGSHFMSLLIRIGVGDELPKI 242

Query: 244 PEELSEEGKDFIGRCFDRDHERRWTADMLLRHPYVREEKEE 278
           PE LSEEGKDF+ +CF +D  +RWTA+MLL H +V  + E+
Sbjct: 243 PEMLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLED 274

BLAST of Spg013703 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 3.3e-39
Identity = 101/272 (37.13%), Postives = 143/272 (52.57%), Query Frame = 0

Query: 4   WVRLKILGEGSYGVVFLAKPTTGTLDNFFAIKATHLQHYSSLSKEKQILKHFIGCPEIVQ 63
           W R +ILG GS   V+ A       D   A+K++ +     L +E +IL   +  P ++ 
Sbjct: 3   WTRGRILGRGSTATVYAAAGHNS--DEILAVKSSEVHRSEFLQREAKILSS-LSSPYVIG 62

Query: 64  YLGSEITIEKYSPDMAFYNLKLEYAAGGTLAD-LIKQKQKLPENEVKQYLKMILRGLSCI 123
           Y GSE   ++ S  +  YNL +EYA  GTL D   K   ++ E  V +Y + IL+GL  I
Sbjct: 63  YRGSE--TKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGRVDETRVVKYTRDILKGLEYI 122

Query: 124 HRKGFVHVDLKPSNILAFPQSDGKTKLKIADFGLSRRRGEDDGEDYRGVPRFKGTPKYMS 183
           H KG VH D+K SN++       K + KIADFG ++R       D        GTP +M+
Sbjct: 123 HSKGIVHCDVKGSNVVI----SEKGEAKIADFGCAKR------VDPVFESPVMGTPAFMA 182

Query: 184 PESVIFSEVKSSLDIWSLGCILVEMVSGRSVWEDCRSRREFVSKLV---CSEEMPEIPEE 243
           PE     +     DIW++GC ++EMV+G   W    SR + VS L     S E PE+P  
Sbjct: 183 PEVARGEKQGKESDIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETPELPCL 242

Query: 244 LSEEGKDFIGRCFDRDHERRWTADMLLRHPYV 272
           L+EE KDF+ +C  R+   RWTA  LL HP++
Sbjct: 243 LAEEAKDFLEKCLKREANERWTATQLLNHPFL 259

BLAST of Spg013703 vs. ExPASy Swiss-Prot
Match: Q54R82 (Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=44689 GN=mkkA PE=1 SV=2)

HSP 1 Score: 152.5 bits (384), Expect = 7.7e-36
Identity = 96/282 (34.04%), Postives = 148/282 (52.48%), Query Frame = 0

Query: 4   WVRLKILGEGSYGVVFLA-KPTTGTLDNFFAIKATHLQHYSSLSKEKQILKHF------- 63
           W + +ILG G YG V+L     TG L   FA+K   +   +S  K K ++  F       
Sbjct: 170 WQKGQILGRGGYGSVYLGLNKDTGEL---FAVKQLEIVDINSDPKLKNMILSFSKEIEVM 229

Query: 64  --IGCPEIVQYLGSEITIEKYSPDMAFYNLKLEYAAGGTLADLIKQKQKLPENEVKQYLK 123
             +    IV+YLG+ +       D +F ++ LEY  GG+++ L+ +     EN +K Y K
Sbjct: 230 RSLRHDNIVRYLGTSL-------DQSFLSVFLEYIPGGSISSLLGKFGAFSENVIKVYTK 289

Query: 124 MILRGLSCIHRKGFVHVDLKPSNILAFPQSDGKTKLKIADFGLSRRRGEDDGEDYRG-VP 183
            IL+GLS +H    +H D+K +NIL     D K  +K++DFG S+         + G V 
Sbjct: 290 QILQGLSFLHANSIIHRDIKGANILI----DTKGIVKLSDFGCSK--------SFSGIVS 349

Query: 184 RFK---GTPKYMSPESVIFSEVKSSLDIWSLGCILVEMVSGRSVWEDCRSRREFVSKLVC 243
           +FK   GTP +M+PE +  +    S DIWSLGC++VEM + +  W +       +  +  
Sbjct: 350 QFKSMQGTPYWMAPEVIKQTGHGRSSDIWSLGCVIVEMATAQPPWSNITELAAVMYHIAS 409

Query: 244 SEEMPEIPEELSEEGKDFIGRCFDRDHERRWTADMLLRHPYV 272
           S  +P IP  +S+E  DF+  CF RD + R  A+ LL+HP++
Sbjct: 410 SNSIPNIPSHMSQEAFDFLNLCFKRDPKERPDANQLLKHPFI 429

BLAST of Spg013703 vs. ExPASy Swiss-Prot
Match: Q9ZVP5 (Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK18 PE=1 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 2.2e-35
Identity = 98/291 (33.68%), Postives = 145/291 (49.83%), Query Frame = 0

Query: 3   NWVRLKILGEGSYGVVFLAKPTTGTLDNFFAIKATHLQHYSSLSKEKQILKHFIGCPEIV 62
           NW R K LG GS   V  A  T        A+K+        L +E +IL   +  P ++
Sbjct: 2   NWTRGKTLGRGSTATVSAA--TCHESGETLAVKSAEFHRSEFLQREAKILSS-LNSPYVI 61

Query: 63  QYLGSEITIEKY--SPDMAFYNLKLEYAAGGTLADL-IKQKQKLPENEVKQYLKMILRGL 122
            Y G EIT E +  + +   Y+L +EYA  GTL D+  K    + E  V +Y + IL GL
Sbjct: 62  GYRGCEITREPFHNNGEATTYSLLMEYAPYGTLTDVATKNGGFIDEARVVKYTRQILLGL 121

Query: 123 SCIHR-KGFVHVDLKPSNILAFPQSDGKTKLKIADFGLSRRRGEDDGEDYRGVPRFKGTP 182
             IH  KG  H D+K SN+L     +     KIADFG ++    +  E  R      GTP
Sbjct: 122 EYIHNSKGIAHCDIKGSNVLVGENGEA----KIADFGCAKWVEPEITEPVR------GTP 181

Query: 183 KYMSPESVIFSEVKSSLDIWSLGCILVEMVSGRSVW--EDCRSRREFVSKLVCSEEMPEI 242
            +M+PE+          DIW++GC ++EMV+G   W   D       + ++    E+PE+
Sbjct: 182 AFMAPEAARGERQGKESDIWAVGCTVIEMVTGSQPWIGADFTDPVSVLYRVGYLGELPEL 241

Query: 243 PEELSEEGKDFIGRCFDRDHERRWTADMLLRHPYVREEKEEGMTAKLPGRP 288
           P  L+E+ KDF+G+C  ++   RWTA  LL HP++  ++ E +T  +   P
Sbjct: 242 PCSLTEQAKDFLGKCLKKEATERWTASQLLNHPFLVNKEPELVTGLVTNSP 279

BLAST of Spg013703 vs. ExPASy Swiss-Prot
Match: Q40541 (Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097 GN=NPK1 PE=1 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 1.5e-34
Identity = 88/285 (30.88%), Postives = 147/285 (51.58%), Query Frame = 0

Query: 4   WVRLKILGEGSYGVVFLAKPTTGTLDNFFAIKATHL-----------QHYSSLSKEKQIL 63
           W + +++G G++G V++            AIK   +            H   L +E  +L
Sbjct: 80  WRKGEMIGCGAFGRVYMGMNVDS--GELLAIKEVSIAMNGASRERAQAHVRELEEEVNLL 139

Query: 64  KHFIGCPEIVQYLGSEITIEKYSPDMAFYNLKLEYAAGGTLADLIKQKQKLPENEVKQYL 123
           K+ +  P IV+YLG+       + +    N+ LE+  GG+++ L+ +    PE+ ++ Y 
Sbjct: 140 KN-LSHPNIVRYLGT-------AREAGSLNILLEFVPGGSISSLLGKFGSFPESVIRMYT 199

Query: 124 KMILRGLSCIHRKGFVHVDLKPSNILAFPQSDGKTKLKIADFGLSRRRGEDDGEDYRGVP 183
           K +L GL  +H+ G +H D+K +NIL     D K  +K+ADFG S++  E       G  
Sbjct: 200 KQLLLGLEYLHKNGIMHRDIKGANILV----DNKGCIKLADFGASKKVVE--LATMTGAK 259

Query: 184 RFKGTPKYMSPESVIFSEVKSSLDIWSLGCILVEMVSGRSVWEDCRSRREFVSKLVCSEE 243
             KGTP +M+PE ++ +    S DIWS+GC ++EM +G+  W         +  +  ++ 
Sbjct: 260 SMKGTPYWMAPEVILQTGHSFSADIWSVGCTIIEMATGKPPWSQQYQEVAALFHIGTTKS 319

Query: 244 MPEIPEELSEEGKDFIGRCFDRDHERRWTADMLLRHPYVREEKEE 278
            P IPE LS E KDF+ +C  ++   R +A  LL+HP+V  E +E
Sbjct: 320 HPPIPEHLSAESKDFLLKCLQKEPHLRHSASNLLQHPFVTAEHQE 348

BLAST of Spg013703 vs. ExPASy TrEMBL
Match: A0A6J1D9A6 (mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX=3673 GN=LOC111018431 PE=4 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 3.9e-99
Identity = 192/308 (62.34%), Postives = 234/308 (75.97%), Query Frame = 0

Query: 1   MTNWVRLKILGEGSYGVVFLAKPTTGTLDNFFAIKATHLQHYSSLSKEKQILKHFIGCPE 60
           MTNWVR+  LG+GSYG VFLAKPTT +L  +FA+K   LQHYSSL  EK+ILKHF+G PE
Sbjct: 1   MTNWVRVXDLGKGSYGKVFLAKPTTRSL-KYFAVKVAELQHYSSLLNEKRILKHFVGFPE 60

Query: 61  IVQYLGSEITIEKYSPDMAFYNLKLEYAAGGTLADLIKQKQKLPENEVKQYLKMILRGLS 120
           IVQ  G E+  E  S D AFY+L LEYAAGGTLADLI++++KL EN VK+YLKMILRGLS
Sbjct: 61  IVQCFGGEMITEGDSFDSAFYSLVLEYAAGGTLADLIRKQKKLSENTVKEYLKMILRGLS 120

Query: 121 CIHRKGFVHVDLKPSNILAFPQSDGKTKLKIADFGLSRRRGEDDGEDYRGVPRFKGTPKY 180
           CIH+ GFVHVDLKP+NILAFPQ DGK KLKIADFGLSRR  +    +++   +F+GTP Y
Sbjct: 121 CIHKLGFVHVDLKPANILAFPQIDGKMKLKIADFGLSRRCNKKRECNFK--LKFRGTPMY 180

Query: 181 MSPESVIFSEVKSSLDIWSLGCILVEMVSGRSVWEDCRSRREFVSKLVCSEEMPEIPEEL 240
           MSPES I+ EV + LDIWSLGCILVEMV+G+  W+DC    + + KL  + E P+IPEEL
Sbjct: 181 MSPES-IYGEVNTPLDIWSLGCILVEMVTGKHAWDDCIDLEQLMLKLRVNMETPKIPEEL 240

Query: 241 SEEGKDFIGRCFDRDHERRWTADMLLRHPYVREEKEEGMTAKLPGRPKFPKGIYLPHLQC 300
           S+EGKDF+ +CFDR+ +RRWTADMLL+HPY+ +EKE     K    P +    YLPH+  
Sbjct: 241 SKEGKDFLEKCFDRNPKRRWTADMLLQHPYLEQEKE---AIKKDDDPTYS---YLPHILY 298

Query: 301 KLRSCIQI 309
           K +S IQI
Sbjct: 301 KFQSTIQI 298

BLAST of Spg013703 vs. ExPASy TrEMBL
Match: A0A0A0K835 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G407720 PE=4 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 9.0e-96
Identity = 188/309 (60.84%), Postives = 230/309 (74.43%), Query Frame = 0

Query: 3   NWVRLKILGEGSYGVVFLAKPTT---GTLDNFFAIKATHLQH-YSSLSKEKQILKHFIGC 62
           +WV +K LGEGS G+V LAK  T     L  +FA+K   L++  SSL  E+ +LKHF  C
Sbjct: 4   DWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLWEEHVLKHFTDC 63

Query: 63  PEIVQYLGSEIT-IEKYSPDMAFYNLKLEYAAGGTLADLIKQKQKLPENEVKQYLKMILR 122
           PEIVQYLGSE+T    +  D   YNLKLEYAAGGTLADLIKQ+ KLPE+EVK+YL+MIL+
Sbjct: 64  PEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQMILK 123

Query: 123 GLSCIHRKGFVHVDLKPSNILAFPQSDGKTKLKIADFGLSRR---RGEDDGED-----YR 182
           GLSCIHRKGFVHVDLKP NILAFPQSDGK KLKIADFGL+ R   RGEDD ED     Y 
Sbjct: 124 GLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGSKYYS 183

Query: 183 GVPRFKGTPKYMSPESVIFSEVKSSLDIWSLGCILVEMVSGRSVWEDCRSRREFVSKLVC 242
           G  + + T +YMSPES++FSE+    DIWSLGC LV+MVSG  VW DC+S  E ++KL+ 
Sbjct: 184 GALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCKSYEELITKLLI 243

Query: 243 SEEMPEIPEELSEEGKDFIGRCFDRDHERRWTADMLLRHPYVREEKEEGMTAKLPGRPKF 298
           SEE+P IPEELS++GKDF+ +CF R++E+RWTADMLL+HPY+ EE ++  T     + K 
Sbjct: 244 SEEIPTIPEELSKQGKDFLEKCFVRNYEQRWTADMLLQHPYLNEENKD--TKNGDEKLKL 303

BLAST of Spg013703 vs. ExPASy TrEMBL
Match: A0A5A7TZP4 (Serine/threonine-protein kinase Chk1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001750 PE=4 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 4.6e-92
Identity = 179/308 (58.12%), Postives = 227/308 (73.70%), Query Frame = 0

Query: 1   MTNWVRLKILGEGSYGVVFLAKPTT---GTLDNFFAIKATHLQHYS-SLSKEKQILKHFI 60
           M +WV LK+LGEGS G+V LAK  T     L  +FA+K   L++ S SL  E+ +LKH  
Sbjct: 1   MEDWVLLKVLGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSASLLWEEHVLKHLT 60

Query: 61  GCPEIVQYLGSEIT-IEKYSPDMAFYNLKLEYAAGGTLADLIKQKQKLPENEVKQYLKMI 120
            CPEIVQYLGSE+T    +  D   YNLKLEYAAGGTLADLIKQ+ KLPE+EVK+YL+MI
Sbjct: 61  DCPEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQMI 120

Query: 121 LRGLSCIHRKGFVHVDLKPSNILAFPQSDGKTKLKIADFGLSRR--RGEDDGED----YR 180
           L+GLSCIHRKGFVHVDLKP NILAFPQSDGK KLKIADFGL+ R   GEDD ED    + 
Sbjct: 121 LKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERFKHGEDDQEDKGCEHY 180

Query: 181 GVPRFKGTPKYMSPESVIFSEVKSSLDIWSLGCILVEMVSGRSVWEDCRSRREFVSKLVC 240
           G  R + T +YMSPES++  E+    DIWSLGC+LV+MVSG  VW DC++  E ++KL+ 
Sbjct: 181 GALRVRATHRYMSPESIVLKEIDGLHDIWSLGCVLVQMVSGERVWNDCKNYEELMTKLLN 240

Query: 241 SEEMPEIPEELSEEGKDFIGRCFDRDHERRWTADMLLRHPYVREEKEEGMTAKLPGRPKF 298
           S+E+P IP+ELS++G DF+ +CF RD+++RWTADMLL+HPY+ E+ ++    +   + K 
Sbjct: 241 SKEIPIIPKELSKQGIDFLEKCFVRDYKQRWTADMLLQHPYLNEQNKDTSDDE---KLKL 300

BLAST of Spg013703 vs. ExPASy TrEMBL
Match: A0A5D3DR57 (Mitogen-activated protein kinase kinase kinase ANP1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold111G00640 PE=4 SV=1)

HSP 1 Score: 347.4 bits (890), Expect = 6.0e-92
Identity = 175/297 (58.92%), Postives = 221/297 (74.41%), Query Frame = 0

Query: 1   MTNWVRLKILGEGSYGVVFLAKPTT-------GTLDNFFAIKATHLQHYSSLSKEKQILK 60
           M NWV +K+LG+GSYGVV LAK ++         L  +FA+K + LQH SSL  E+Q+LK
Sbjct: 1   MENWVPVKVLGQGSYGVVCLAKQSSAIRKKSDSNLPYYFAVKLSPLQHNSSLLWEEQVLK 60

Query: 61  HFIGCPEIVQYLGSEITIEK--YSPDMAFYNLKLEYAAGGTLADLIKQKQKLPENEVKQY 120
            F  CPEIVQYLGSEIT  +   +  + FY LKLEYAAGGTL DLI+Q+ KLPE EVK Y
Sbjct: 61  QFKDCPEIVQYLGSEITRGRDIRNDHVDFYTLKLEYAAGGTLDDLIEQRDKLPEAEVKDY 120

Query: 121 LKMILRGLSCIHRKGFVHVDLKPSNILAFPQSDGKTKLKIADFGLSRRRGEDDGEDYRGV 180
           L+MIL+GLSCIH KGFVHVDLKP+NILAFPQSDGK KLKIADFG + R      + ++  
Sbjct: 121 LRMILKGLSCIHSKGFVHVDLKPNNILAFPQSDGKMKLKIADFGQAERSKYRVDDQHKQY 180

Query: 181 P------RFKGTPKYMSPESVIFSEVKSSLDIWSLGCILVEMVSGRSVWEDCRSRREFVS 240
           P      +FKGTP+YMSPESV+F+EV  + DIWSLGCILV+M+SG+S W+DC+  ++ + 
Sbjct: 181 PPCCSSFKFKGTPRYMSPESVVFNEVNDADDIWSLGCILVKMISGKSAWDDCKDSKQLMI 240

Query: 241 KLVCSEEMPEIPEELSEEGKDFIGRCFDRDHERRWTADMLLRHPYVREEKEEGMTAK 283
           KL+ ++ MP IP ELSE+GKDFI +CF R+++ RWTA+MLL+HPY+ EE E  M  K
Sbjct: 241 KLLDNKIMPTIPGELSEQGKDFIKKCFIRNYKERWTAEMLLQHPYLNEENEAPMNNK 297

BLAST of Spg013703 vs. ExPASy TrEMBL
Match: A0A1S3CQQ0 (mitogen-activated protein kinase kinase kinase ANP1-like OS=Cucumis melo OX=3656 GN=LOC103503238 PE=4 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 3.0e-91
Identity = 174/297 (58.59%), Postives = 220/297 (74.07%), Query Frame = 0

Query: 1   MTNWVRLKILGEGSYGVVFLAKPTT-------GTLDNFFAIKATHLQHYSSLSKEKQILK 60
           M NWV +K+LG+GSY VV LAK ++         L  +FA+K + LQH SSL  E+Q+LK
Sbjct: 1   MENWVPVKVLGQGSYAVVCLAKQSSAIRKKSDNNLPYYFAVKLSPLQHNSSLLWEEQVLK 60

Query: 61  HFIGCPEIVQYLGSEITIEK--YSPDMAFYNLKLEYAAGGTLADLIKQKQKLPENEVKQY 120
            F  CPEIVQYLGSEIT  +   +  + FY LKLEYAAGGTL DLI+Q+ KLPE EVK Y
Sbjct: 61  QFKDCPEIVQYLGSEITRGRDIRNDHVDFYTLKLEYAAGGTLDDLIEQRDKLPEAEVKDY 120

Query: 121 LKMILRGLSCIHRKGFVHVDLKPSNILAFPQSDGKTKLKIADFGLSRRRGEDDGEDYRGV 180
           L+MIL+GLSCIH KGFVHVDLKP+NILAFPQSDGK KLKIADFG + R      + ++  
Sbjct: 121 LRMILKGLSCIHSKGFVHVDLKPNNILAFPQSDGKMKLKIADFGQAERSKYRVDDQHKQY 180

Query: 181 P------RFKGTPKYMSPESVIFSEVKSSLDIWSLGCILVEMVSGRSVWEDCRSRREFVS 240
           P      +FKGTP+YMSPESV+F+EV  + DIWSLGCILV+M+SG+S W+DC+  ++ + 
Sbjct: 181 PPCCSSFKFKGTPRYMSPESVVFNEVNDADDIWSLGCILVKMISGKSAWDDCKDSKQLMI 240

Query: 241 KLVCSEEMPEIPEELSEEGKDFIGRCFDRDHERRWTADMLLRHPYVREEKEEGMTAK 283
           KL+ ++ MP IP ELSE+GKDFI +CF R+++ RWTA+MLL+HPY+ EE E  M  K
Sbjct: 241 KLLDNKIMPTIPGELSEQGKDFIKKCFIRNYKERWTAEMLLQHPYLNEENEAPMNNK 297

BLAST of Spg013703 vs. TAIR 10
Match: AT4G36950.1 (mitogen-activated protein kinase kinase kinase 21 )

HSP 1 Score: 201.1 bits (510), Expect = 1.3e-51
Identity = 116/284 (40.85%), Postives = 169/284 (59.51%), Query Frame = 0

Query: 4   WVRLKILGEGSYGVVFLAKPTTGTLDNFF----AIKATHLQHYSSLSKEKQILKHFIGCP 63
           W+R + +G GS+  V LA  T+G+    F    A+K++ +   ++L  E+ +L     C 
Sbjct: 3   WIRRETIGHGSFSTVSLA-TTSGSSSKAFPSLMAVKSSGVVCSAALRNERDVLDDLGDCS 62

Query: 64  EIVQYLGSEITIEKYSPDMAFYNLKLEYAAGGTLADLIKQK-QKLPENEVKQYLKMILRG 123
           EIV+  G   T+E        YNL LEYA+GG+LAD IK   + LPE EV+++ + I++G
Sbjct: 63  EIVRCFGEGRTVEN---GEEIYNLFLEYASGGSLADRIKSSGEALPEFEVRRFTRSIVKG 122

Query: 124 LSCIHRKGFVHVDLKPSNILAFPQSDGKTKLKIADFGLSRRRGEDDGEDYRGVPRFKGTP 183
           L  IH  GF H D+K  N+L F   D    +KI+DFGL++RR  +   + R      GTP
Sbjct: 123 LCHIHGNGFTHCDIKLENVLVFGDGD----VKISDFGLAKRRSGEVCVEIR------GTP 182

Query: 184 KYMSPESVIFSEVKSSLDIWSLGCILVEMVSGRSVW--ED--CRSRREFVSKLVCSEEMP 243
            YM+PESV   E +S  DIW+LGC +VEM SG++ W  ED    +    + ++   +E+P
Sbjct: 183 LYMAPESVNHGEFESPADIWALGCSVVEMSSGKTAWCLEDGVMNNVMSLLVRIGSGDEVP 242

Query: 244 EIPEELSEEGKDFIGRCFDRDHERRWTADMLLRHPYVREEKEEG 279
            IP ELSEEGKDF+ +CF ++   RWTA+MLL HP++  + E G
Sbjct: 243 RIPVELSEEGKDFVSKCFVKNAAERWTAEMLLDHPFLAVDDESG 272

BLAST of Spg013703 vs. TAIR 10
Match: AT3G50310.1 (mitogen-activated protein kinase kinase kinase 20 )

HSP 1 Score: 199.1 bits (505), Expect = 5.1e-51
Identity = 116/281 (41.28%), Postives = 170/281 (60.50%), Query Frame = 0

Query: 4   WVRLKILGEGSYGVVFLAKPT--TGTLDNFFAIKATHLQHYSSLSKEKQILKHFIGCPEI 63
           WVR + +G G++  V  A  +  +G      A+K+T     +SLS EK +L     CPEI
Sbjct: 3   WVRGETIGFGTFSTVSTATKSRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDCPEI 62

Query: 64  VQYLGSEITIEKYSPDMAFYNLKLEYAAGGTLADLIKQ--KQKLPENEVKQYLKMILRGL 123
           ++  G + T+E        +NL LEYA+ G+LA  +K+   + LPE+ V+++   +LRGL
Sbjct: 63  IRCYGEDSTVEN---GEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGL 122

Query: 124 SCIHRKGFVHVDLKPSNILAFPQSDGKTKLKIADFGLSRRRGEDDGEDYRGVPRFKGTPK 183
             IH KGF H D+K +NIL F  +DG   +KIADFGL+  R + D    R     +GTP 
Sbjct: 123 RHIHAKGFAHCDIKLANILLF--NDG--SVKIADFGLA-MRVDGDLTALRKSVEIRGTPL 182

Query: 184 YMSPESVIFSEVKSSLDIWSLGCILVEMVSGRSVWEDCRSRREFVSKLV---CSEEMPEI 243
           YM+PE V  +E  S+ D+W+LGC +VEM SG++ W   +    F+S L+     +E+P+I
Sbjct: 183 YMAPECVNDNEYGSAADVWALGCAVVEMFSGKTAW-SVKEGSHFMSLLIRIGVGDELPKI 242

Query: 244 PEELSEEGKDFIGRCFDRDHERRWTADMLLRHPYVREEKEE 278
           PE LSEEGKDF+ +CF +D  +RWTA+MLL H +V  + E+
Sbjct: 243 PEMLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLED 274

BLAST of Spg013703 vs. TAIR 10
Match: AT5G67080.1 (mitogen-activated protein kinase kinase kinase 19 )

HSP 1 Score: 194.9 bits (494), Expect = 9.6e-50
Identity = 114/288 (39.58%), Postives = 174/288 (60.42%), Query Frame = 0

Query: 4   WVRLKILGEGSYGVVFLAKPT---TGTLDNFFAIKATHLQHYSSLSKEKQILKHF-IGCP 63
           W+R + +G G++  V LA  +   +G      A+K+      +SL+ EK +L +    C 
Sbjct: 3   WIRGETIGYGTFSTVSLATRSNNDSGEFPPLMAVKSADSYGAASLANEKSVLDNLGDDCN 62

Query: 64  EIVQYLGSEITIEKYSPDMAFYNLKLEYAAGGTLADLIKQ--KQKLPENEVKQYLKMILR 123
           EIV+  G + T+E        +NL LEYA+ G+L   +K+   + +PE+ V+++   +LR
Sbjct: 63  EIVRCFGEDRTVEN---GEEMHNLFLEYASRGSLESYLKKLAGEGVPESTVRRHTGSVLR 122

Query: 124 GLSCIHRKGFVHVDLKPSNILAFPQSDGKTKLKIADFGLSRRRGEDDGEDYRGVPRFKGT 183
           GL  IH  GF H DLK  NIL F    G   +KIADFGL++R G+    +Y GV + +GT
Sbjct: 123 GLRHIHANGFAHCDLKLGNILLF----GDGAVKIADFGLAKRIGDLTALNY-GV-QIRGT 182

Query: 184 PKYMSPESVIFSEVKSSLDIWSLGCILVEMVSGRSVWEDCRSRREFVSKLV---CSEEMP 243
           P YM+PESV  +E  S  D+W+LGC++VEM SG++ W   +    F+S L+     +E+P
Sbjct: 183 PLYMAPESVNDNEYGSEGDVWALGCVVVEMFSGKTAW-SLKEGSNFMSLLLRIGVGDEVP 242

Query: 244 EIPEELSEEGKDFIGRCFDRDHERRWTADMLLRHPYVREEKEEGMTAK 283
            IPEELSE+G+DF+ +CF +D ++RWTA+MLL HP+V  + +  +  K
Sbjct: 243 MIPEELSEQGRDFLSKCFVKDPKKRWTAEMLLNHPFVTVDVDHDVLVK 280

BLAST of Spg013703 vs. TAIR 10
Match: AT2G41930.1 (Protein kinase superfamily protein )

HSP 1 Score: 185.7 bits (470), Expect = 5.8e-47
Identity = 111/281 (39.50%), Postives = 168/281 (59.79%), Query Frame = 0

Query: 7   LKILGEGSYGVV--FLAKPTTGTLDNFFAIKATHLQHYSSLSKEKQILKHFIGCPEIVQY 66
           +K+LG+G+YG V  F  K   G+L  + A+K    ++Y S+ +E +IL    GCP IVQ 
Sbjct: 8   VKVLGKGTYGSVELFSHKQNDGSL-LYNAVKIMDSENYGSIDQEFRILSELRGCPCIVQL 67

Query: 67  LGSEITIEKYSPDMAFYNLKLEYAAGGTLADLIKQ-KQKLPENEVKQYLKMILRGLSCIH 126
            G+ +           Y + +EYAA GTL + IK+ + KL ++ +K + +MIL+GL  IH
Sbjct: 68  CGNSLVQGIDCNGKKVYMMSMEYAAAGTLTNFIKRNRTKLSDSVIKDFTRMILQGLVSIH 127

Query: 127 RKGFVHVDLKPSNILAFPQSDGKT-----KLKIADFGLSRRRGEDDGEDYRGVPRFKGTP 186
             G+VH DLKP NIL FP  D  T     +LKI+DFG+S R G+  G  +R    + GT 
Sbjct: 128 NHGYVHCDLKPDNILLFPLYDKDTWNCSYELKISDFGISTRAGDKSG-CWRVDEPWVGTS 187

Query: 187 KYMSPESVI-FSEVKSSLDIWSLGCILVEMVSGRSVWEDCRSRREFVSKLVCSEEMPEIP 246
            YMSPESV   + V+ +LD+WSLGCI+++M +G+  W       + V  L+ +++ PEIP
Sbjct: 188 IYMSPESVSDGTTVEKTLDLWSLGCIVLKMYTGKRPW---LGFEKDVKSLLLNQKAPEIP 247

Query: 247 EELSEEGKDFIGRCFDRDHERRWTADMLLRHPYVREEKEEG 279
           E L  + + F+ +CF R  E R +A  LL HP++  ++++G
Sbjct: 248 ETLPCDARLFLEKCFSRKPEERGSASELLLHPFLTGDEKKG 283

BLAST of Spg013703 vs. TAIR 10
Match: AT4G26890.1 (mitogen-activated protein kinase kinase kinase 16 )

HSP 1 Score: 183.3 bits (464), Expect = 2.9e-46
Identity = 103/292 (35.27%), Postives = 159/292 (54.45%), Query Frame = 0

Query: 3   NWVRLKILGEGSYGVVFLAKPTTGTLDNFFAIKATHLQHYSSLSKEKQILKHFIGCPEIV 62
           NW R  I+G GS   V +A  ++G L   FA+K+  L   S L KE+ IL   +  P +V
Sbjct: 4   NWTRGPIIGRGSTATVSIAISSSGEL---FAVKSADLSSSSLLQKEQSILS-TLSSPHMV 63

Query: 63  QYLGSEITIEKYSPDMAFYNLKLEYAAGGTLADLIKQK-QKLPENEVKQYLKMILRGLSC 122
           +Y+G+ +T E    +   YN+ +EY +GG L DLIK    KLPE E++ Y + IL GL  
Sbjct: 64  KYIGTGLTRES---NGLVYNILMEYVSGGNLHDLIKNSGGKLPEPEIRSYTRQILNGLVY 123

Query: 123 IHRKGFVHVDLKPSNILAFPQSDGKTKLKIADFGLSRRRGEDDGEDYRGVPRFKGTPKYM 182
           +H +G VH DLK  N+L     +    LKIAD G ++   + +         F GTP +M
Sbjct: 124 LHERGIVHCDLKSHNVLV----EENGVLKIADMGCAKSVDKSE---------FSGTPAFM 183

Query: 183 SPESVIFSEVKSSLDIWSLGCILVEMVSGRSVWEDCRSRREFVSKLVCSEEMPEIPEELS 242
           +PE     E +   D+W+LGC ++EM++G S W +       + K+  S E P IP  +S
Sbjct: 184 APEVARGEEQRFPADVWALGCTMIEMMTGSSPWPELNDVVAAMYKIGFSGESPAIPAWIS 243

Query: 243 EEGKDFIGRCFDRDHERRWTADMLLRHPYVREEKEEGMTAKLPGRPKFPKGI 294
           ++ KDF+  C   D ++RWT + LL+HP++ +++E   +  L  +   P  +
Sbjct: 244 DKAKDFLKNCLKEDQKQRWTVEELLKHPFLDDDEESQTSDCLKNKTSSPSTV 275

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022150204.18.0e-9962.34mitogen-activated protein kinase kinase kinase 17-like [Momordica charantia][more]
XP_038896325.14.0e-9861.79mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida][more]
XP_004136151.11.9e-9560.84mitogen-activated protein kinase kinase kinase 17 [Cucumis sativus] >KGN45019.1 ... [more]
KAA0046699.19.5e-9258.12serine/threonine-protein kinase Chk1-like [Cucumis melo var. makuwa] >TYK18235.1... [more]
KAA0059705.11.2e-9158.92mitogen-activated protein kinase kinase kinase ANP1-like [Cucumis melo var. maku... [more]
Match NameE-valueIdentityDescription
Q9SND67.2e-5041.28Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370... [more]
O808883.3e-3937.13Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=370... [more]
Q54R827.7e-3634.04Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=... [more]
Q9ZVP52.2e-3533.68Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=370... [more]
Q405411.5e-3430.88Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097... [more]
Match NameE-valueIdentityDescription
A0A6J1D9A63.9e-9962.34mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX... [more]
A0A0A0K8359.0e-9660.84Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G407... [more]
A0A5A7TZP44.6e-9258.12Serine/threonine-protein kinase Chk1-like OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A5D3DR576.0e-9258.92Mitogen-activated protein kinase kinase kinase ANP1-like OS=Cucumis melo var. ma... [more]
A0A1S3CQQ03.0e-9158.59mitogen-activated protein kinase kinase kinase ANP1-like OS=Cucumis melo OX=3656... [more]
Match NameE-valueIdentityDescription
AT4G36950.11.3e-5140.85mitogen-activated protein kinase kinase kinase 21 [more]
AT3G50310.15.1e-5141.28mitogen-activated protein kinase kinase kinase 20 [more]
AT5G67080.19.6e-5039.58mitogen-activated protein kinase kinase kinase 19 [more]
AT2G41930.15.8e-4739.50Protein kinase superfamily protein [more]
AT4G26890.12.9e-4635.27mitogen-activated protein kinase kinase kinase 16 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 4..271
e-value: 5.1E-65
score: 232.0
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 6..271
e-value: 2.6E-54
score: 184.4
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 4..271
score: 41.332775
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 1..288
e-value: 5.7E-63
score: 215.1
NoneNo IPR availablePIRSRPIRSR000605-51PIRSR000605-51coord: 7..262
e-value: 5.1E-22
score: 76.1
NoneNo IPR availablePIRSRPIRSR037921-2PIRSR037921-2coord: 7..217
e-value: 1.6E-9
score: 35.3
NoneNo IPR availablePIRSRPIRSR000620-2PIRSR000620-2coord: 8..261
e-value: 8.2E-12
score: 42.6
NoneNo IPR availablePANTHERPTHR48011:SF51PROTEIN KINASE SUPERFAMILY PROTEINcoord: 1..279
NoneNo IPR availablePANTHERPTHR48011CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATEDcoord: 1..279
NoneNo IPR availableCDDcd06606STKc_MAPKKKcoord: 3..271
e-value: 2.42522E-96
score: 283.257
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 10..35
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 3..277

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg013703.1Spg013703.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function GO:0004672 protein kinase activity