Spg013678 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg013678
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	tRNA (cytidine(34)-2'-O)-methyltransferase
Location	scaffold3: 46447607 .. 46450455 (-)
RNA-Seq Expression	Spg013678
Synteny	Spg013678

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCTGAGATTCGAAGCAAGCTTCGTCAGAGAGACACACAGTCTATAGCAGAAGAGCGACAGCGAGAAGAAGAAGATTTCTTAGCTAAAAATGCCATTCATGGACACAACAACAATCACTCTCAGAGCTTTGCACCCATTTTCGTCGAGGGCTCCTCATCTTCTTCGCCCTTACTGTTTTCCCCATTTCAGCCACAAACAGCTCCATCCGTGTTCTTCTTCCTCTCGTCGGCTCCTCCCCTTCTGCTCCTTCTGTGAGTTTATATCTGTAATTAAATGTTCTTGCATCCTCTTCAATCTGTAATTTCAGCTGTGGATTGGGTTGAAGTGCTTTTTTGAGTTTGATTTTGATGTGCTAATGGCTCAGCGGAAAGAGGGGAGGGTTCTAGTTCCCTGCCGCGCGGTATTGGTGAGGCCGTCAATGGGGTTTCGCAGCCAAAGCTTCTTCAAGTTGTATTGGTCTCTCCCCTGGTAATTACGAAGCACATAGCCAGTGCCCTTATCCATATTACTTGAAATTGCTTAGCTGATTCTTTTTCTTTCTCTTCTTTTCTGCCCTCTTCTTTCAAGTCGTTGCTATCTTCAGCTATTGTTTTTGTTCCTAATTGCATTTGGGGGGTCGTTTGGTCTGGCTTGATTGAAAACTTTGAAGAAGTTTCACATTGGTTTAACCTCTGTTTGATTATCAAGGACGAAATTTCTTATGGTTGGAAAATTTTGATCACATGTGAAATGGCTGTATATTTAAGAACTTTAAAGTTAGCTGTGACTTGCAGCTAATACAGTAGGGAAGCTTCGAACCTTGGGGTGAGCTTAATAAGAAAAACCTTTGATGTCTCCTAGTCTGGGCACGGGTGCCCCAAATTAGTGGGGGCGAGGCCCTCGATATTAGGTTATCAAAAAATATAAGGTAGCTTTTCTTAGATTCTGCATGTTCTTTGAATCACTGTTCACCATTTTTATTTGAACTTCTCATCTAGACAAACAAAAGAAATTCAGAGAACTCTAGCGTAGGGAAATTTGCAACATTCAAAGTGGTGTTTTGTATGAAAGCAAATCGATAGGGAACCTCTGATTAGATATTTGTATAAATCACTGTGGTGTGCGAAAGTCTGATGGTAAACCAGAGATGTTAGGTGATAGTATTAGATGATTGGCAAGTTGGAATTAGTAGAGCTGCTGGCAATTGACATTGACAAAGCCTGCTTGGCAACAGTCTTCTTCAAATTGAGTGAGGATCGAGGAAACATTGTCGAACAGAAATAACAATAGATTGCTCGAAAACTTGTGGGGCGTATTTGTTACTGAGGTCACATTAGAGCTAAAGATTGTGTGATGTGAAGCATAAACTTTTATTTTGGTTTATACGTGTGTACACAATTGTTCAGCTTATTAGTTTTTATATCATGGCACGAGTTATATGCAGTTGTGTGAAACGCGTTGAACTCCAAATCATAATTTCTCCATTTTCATTTTTGGGTTGCAGACAACCTCAAGGCTGGCTCTGAGATTTCTGTTGTCCTTGACAGATTCCTGGGAACACTGGCAGCATTGCCAGAACATGTGCTGCCTCAGCTGTTGGACTACATCTAGTTGGGGTTAGTATATTGTAGCATCGCTACTTGAGTGCAATTGCTCTGATTTCCCCTTCTAAATTCTAGCTGAAGATCCCCAAAAAGTTATGTATGGTGCTGCTACTGACTTAATAACTTATCTGCTTGTGTCGTTTCTCTCTCTCTTTCTTTTTCAGCCATTGGGATTTAAGGTGGATGACACGAAGTTAAAGCGGGCTGGTTTAGATTACTGGCCGTATCCCATGATGCATTATGAACATGATTATATTGCTCTCATCTACAGTTAAAGATTAGGGATTCGAGTTCTTTGAATGTTAGGAAATTGTGCTTTACATTTACTTGAACTGTTTGAGTTTGCTATTTTCTTGATTATAGATTCCTTCCGCACCTTCATTTTGCTTTCCTCCTTAAATTTTATTTCCCATTTTGAACTGAGATTTGTTAAACCAATCTGTCACTATACATACCAATGTTTTCAAGTATTAACATCATGTTTTAGATACGTCGTCGTTAAAATTCATAGCACGTGGGCAGAGTTCCGAGACTACTTCAATAAACAGGTAATCTTCATTCACACACTTATCCCAGACCAACTTTAAATTGCTTTTGTCTCATGTCTGTATAGATCCTATTCATTGATCTATGAAAATGTGCAAAAAGAAACTCTATTTGCTTGCCTCTTCAATGAGTTTCTTCCTGCATCCTGCACTGTCAAGTTATATCTTCCAATCCAATCGATACGTACAGGAAGGAGAGAAGCGGCTACTAGCATTTACAAAGAGAGGAACTGCACTTCACTCGGCAAGTGCCTATTTCTCTGATGATTTTTATTCCCAAAAACGTCAATTATGATCGTTCTCTTTTCATTTCTGCAGGATTTTTCGTACAAACGGGGCGATTACTTGTTGTTTGGCTCGGAAACTAGTGGCTTACCACCTGAAGCTCTTGAGGACTGCAAAAACCAGCCATTTGGTGGAGGGACCATAAGAATTCCAATGGTTGAAACTTATGTTAGATGCCTCAATCTTTCTGTAAGTGTTGGCATTGCTATATATGAAGCTTCAAGACAACTAAATTACGAGCAGCTTCAGTTTGCACCAGAGGGTTGTACTAACAATGATCAATCTATCATTACGGAGGATCTTTTTGCTTGATTCTTTGATATGAAATTTTGTACCATTTTGAATTGATTTGAAGACAGCAAAGAAGCACGTGCTGTGAAAAGTGGAAGAAACATTGTTTACCTAAATGATGAAATACTTATCTGATGATTAG

mRNA sequence

ATGCCTGAGATTCGAAGCAAGCTTCGTCAGAGAGACACACAGTCTATAGCAGAAGAGCGACAGCGAGAAGAAGAAGATTTCTTAGCTAAAAATGCCATTCATGGACACAACAACAATCACTCTCAGAGCTTTGCACCCATTTTCGTCGAGGGCTCCTCATCTTCTTCGCCCTTACTGTTTTCCCCATTTCAGCCACAAACAGCTCCATCCGTGTTCTTCTTCCTCTCGTCGGCTCCTCCCCTTCTGCTCCTTCTAGGGGAGGGTTCTAGTTCCCTGCCGCGCGGTATTGGTGAGGCCGTCAATGGGGTTTCGCAGCCAAAGCTTCTTCAAGTTGTATTGGTCTCTCCCCTGATTCCTGGGAACACTGGCAGCATTGCCAGAACATGTGCTGCCTCAGCTGTTGGACTACATCTAGTTGGGCCATTGGGATTTAAGGTGGATGACACGAAGTTAAAGCGGGCTGGTTTAGATTACTGGCCATACGTCGTCGTTAAAATTCATAGCACGTGGGCAGAGTTCCGAGACTACTTCAATAAACAGGATTTTTCGTACAAACGGGGCGATTACTTGTTGTTTGGCTCGGAAACTAGTGGCTTACCACCTGAAGCTCTTGAGGACTGCAAAAACCAGCCATTTGGTGGAGGGACCATAAGAATTCCAATGGTTGAAACTTATGTTAGATGCCTCAATCTTTCTGTAAGTGTTGGCATTGCTATATATGAAGCTTCAAGACAACTAAATTACGAGCAGCTTCAGTTTGCACCAGAGGGTTGTACTAACAATGATCAATCTATCATTACGGAGGATCTTTTTGCTTGA

Coding sequence (CDS)

ATGCCTGAGATTCGAAGCAAGCTTCGTCAGAGAGACACACAGTCTATAGCAGAAGAGCGACAGCGAGAAGAAGAAGATTTCTTAGCTAAAAATGCCATTCATGGACACAACAACAATCACTCTCAGAGCTTTGCACCCATTTTCGTCGAGGGCTCCTCATCTTCTTCGCCCTTACTGTTTTCCCCATTTCAGCCACAAACAGCTCCATCCGTGTTCTTCTTCCTCTCGTCGGCTCCTCCCCTTCTGCTCCTTCTAGGGGAGGGTTCTAGTTCCCTGCCGCGCGGTATTGGTGAGGCCGTCAATGGGGTTTCGCAGCCAAAGCTTCTTCAAGTTGTATTGGTCTCTCCCCTGATTCCTGGGAACACTGGCAGCATTGCCAGAACATGTGCTGCCTCAGCTGTTGGACTACATCTAGTTGGGCCATTGGGATTTAAGGTGGATGACACGAAGTTAAAGCGGGCTGGTTTAGATTACTGGCCATACGTCGTCGTTAAAATTCATAGCACGTGGGCAGAGTTCCGAGACTACTTCAATAAACAGGATTTTTCGTACAAACGGGGCGATTACTTGTTGTTTGGCTCGGAAACTAGTGGCTTACCACCTGAAGCTCTTGAGGACTGCAAAAACCAGCCATTTGGTGGAGGGACCATAAGAATTCCAATGGTTGAAACTTATGTTAGATGCCTCAATCTTTCTGTAAGTGTTGGCATTGCTATATATGAAGCTTCAAGACAACTAAATTACGAGCAGCTTCAGTTTGCACCAGAGGGTTGTACTAACAATGATCAATCTATCATTACGGAGGATCTTTTTGCTTGA

Protein sequence

MPEIRSKLRQRDTQSIAEERQREEEDFLAKNAIHGHNNNHSQSFAPIFVEGSSSSSPLLFSPFQPQTAPSVFFFLSSAPPLLLLLGEGSSSLPRGIGEAVNGVSQPKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKVDDTKLKRAGLDYWPYVVVKIHSTWAEFRDYFNKQDFSYKRGDYLLFGSETSGLPPEALEDCKNQPFGGGTIRIPMVETYVRCLNLSVSVGIAIYEASRQLNYEQLQFAPEGCTNNDQSIITEDLFA

Homology

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BLAST of Spg013678 vs. NCBI nr
Match: XP_004135918.1 (uncharacterized protein LOC101203702 [Cucumis sativus] >KGN45148.1 hypothetical protein Csa_016173 [Cucumis sativus])

HSP 1 Score: 345.1 bits (884), Expect = 5.4e-91
Identity = 169/204 (82.84%), Postives = 179/204 (87.75%), Query Frame = 0


Query: 87  EGSSSLPRGIGEAVNGVSQPKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKV 146
           EGS SLP G+GEAVNGVS PKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKV
Sbjct: 65  EGSGSLPTGVGEAVNGVSHPKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKV 124

Query: 147 DDTKLKRAGLDYWPYVVVKIHSTWAEFRDYFNKQ------------------DFSYKRGD 206
           DDTKLKRAGLDYWPYVVVKIH+TWA+F++YF KQ                  DFSYKRGD
Sbjct: 125 DDTKLKRAGLDYWPYVVVKIHNTWADFQEYFKKQEGEKRLLAFTKRGTTHHSDFSYKRGD 184

Query: 207 YLLFGSETSGLPPEALEDCKNQPFGGGTIRIPMVETYVRCLNLSVSVGIAIYEASRQLNY 266
           YLLFGSET+GLPPEALEDCKNQPFGGGT+RIPMVETYVRCLNLSVSVGIAIYEASRQLNY
Sbjct: 185 YLLFGSETTGLPPEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASRQLNY 244

Query: 267 EQLQFAPEGCTNNDQSIITEDLFA 273
           EQLQ + E CTNN+QSIITED+FA
Sbjct: 245 EQLQVSSESCTNNEQSIITEDVFA 268

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BLAST of Spg013678 vs. NCBI nr
Match: XP_022145830.1 (uncharacterized protein LOC111015191 [Momordica charantia])

HSP 1 Score: 343.2 bits (879), Expect = 2.1e-90
Identity = 165/205 (80.49%), Postives = 180/205 (87.80%), Query Frame = 0


Query: 86  GEGSSSLPRGIGEAVNGVSQPKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFK 145
           GEG +SLPRG+GE++NGVS PKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFK
Sbjct: 61  GEGYASLPRGVGESINGVSHPKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFK 120

Query: 146 VDDTKLKRAGLDYWPYVVVKIHSTWAEFRDYFNKQ------------------DFSYKRG 205
           VDDTKLKRAGLDYWPYVV+KIHSTWA+F+DYF KQ                  +FSY+RG
Sbjct: 121 VDDTKLKRAGLDYWPYVVIKIHSTWADFQDYFKKQEGEKRLLAFTKRGTAIHSEFSYRRG 180

Query: 206 DYLLFGSETSGLPPEALEDCKNQPFGGGTIRIPMVETYVRCLNLSVSVGIAIYEASRQLN 265
           DYLLFGSETSGLP EALEDCKNQPFGGGT+RIPMVETYVRCLNLSVSVGIA+YEASRQLN
Sbjct: 181 DYLLFGSETSGLPAEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAVYEASRQLN 240

Query: 266 YEQLQFAPEGCTNNDQSIITEDLFA 273
           YEQLQF  EGC N++QS+ITED+FA
Sbjct: 241 YEQLQFPAEGCNNDEQSVITEDVFA 265

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BLAST of Spg013678 vs. NCBI nr
Match: KAA0058644.1 (putative tRNA (cytidine(34)-2'-O)-methyltransferase [Cucumis melo var. makuwa] >TYK10451.1 putative tRNA (cytidine(34)-2'-O)-methyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 342.4 bits (877), Expect = 3.5e-90
Identity = 167/204 (81.86%), Postives = 179/204 (87.75%), Query Frame = 0


Query: 87  EGSSSLPRGIGEAVNGVSQPKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKV 146
           EGS SLP G+GEAVNGVS PK+LQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKV
Sbjct: 113 EGSGSLPSGVGEAVNGVSHPKILQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKV 172

Query: 147 DDTKLKRAGLDYWPYVVVKIHSTWAEFRDYFNKQ------------------DFSYKRGD 206
           DDTKLKRAGLDYWPYVVVKIH+TWA+F++YF KQ                  DFSYKRGD
Sbjct: 173 DDTKLKRAGLDYWPYVVVKIHNTWADFQEYFKKQEGEKRLLAFTKRGTTHHSDFSYKRGD 232

Query: 207 YLLFGSETSGLPPEALEDCKNQPFGGGTIRIPMVETYVRCLNLSVSVGIAIYEASRQLNY 266
           YLLFGSET+GLPPEALEDCKNQPFGGGT+RIPMVETYVRCLNLSVSVGIAIYEASRQLNY
Sbjct: 233 YLLFGSETTGLPPEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASRQLNY 292

Query: 267 EQLQFAPEGCTNNDQSIITEDLFA 273
           EQLQ + E CTN++QSIITED+FA
Sbjct: 293 EQLQVSSESCTNDEQSIITEDVFA 316

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BLAST of Spg013678 vs. NCBI nr
Match: XP_008461270.1 (PREDICTED: putative tRNA (cytidine(34)-2'-O)-methyltransferase [Cucumis melo])

HSP 1 Score: 342.4 bits (877), Expect = 3.5e-90
Identity = 167/204 (81.86%), Postives = 179/204 (87.75%), Query Frame = 0


Query: 87  EGSSSLPRGIGEAVNGVSQPKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKV 146
           EGS SLP G+GEAVNGVS PK+LQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKV
Sbjct: 65  EGSGSLPSGVGEAVNGVSHPKILQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKV 124

Query: 147 DDTKLKRAGLDYWPYVVVKIHSTWAEFRDYFNKQ------------------DFSYKRGD 206
           DDTKLKRAGLDYWPYVVVKIH+TWA+F++YF KQ                  DFSYKRGD
Sbjct: 125 DDTKLKRAGLDYWPYVVVKIHNTWADFQEYFKKQEGEKRLLAFTKRGTTHHSDFSYKRGD 184

Query: 207 YLLFGSETSGLPPEALEDCKNQPFGGGTIRIPMVETYVRCLNLSVSVGIAIYEASRQLNY 266
           YLLFGSET+GLPPEALEDCKNQPFGGGT+RIPMVETYVRCLNLSVSVGIAIYEASRQLNY
Sbjct: 185 YLLFGSETTGLPPEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASRQLNY 244

Query: 267 EQLQFAPEGCTNNDQSIITEDLFA 273
           EQLQ + E CTN++QSIITED+FA
Sbjct: 245 EQLQVSSESCTNDEQSIITEDVFA 268

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BLAST of Spg013678 vs. NCBI nr
Match: XP_023004870.1 (uncharacterized protein LOC111498049 [Cucurbita maxima])

HSP 1 Score: 341.3 bits (874), Expect = 7.8e-90
Identity = 195/296 (65.88%), Postives = 216/296 (72.97%), Query Frame = 0


Query: 5   RSKLRQRDTQSIAEERQREEEDFLAKNAIHGHNNNHSQS---FAPIFVEGSSSSSPLLFS 64
           R +   R+ +S AEER+R+EED  AK  +        ++   F+P  +  S+S   L   
Sbjct: 24  RPEQLNREARSTAEERRRQEEDCSAKMPLMDTTATTLRTLHPFSPFLLRSSTSHRLLRAR 83

Query: 65  PFQPQTAPSVFFFLSSAPPLLLLL-----GEGSS-SLPRGIGEAVNGVSQPKLLQVVLVS 124
            F   +        SS+  LL        GEGSS SLPRG+GEA+NGVSQ K+LQVVLVS
Sbjct: 84  CFSHFSHKHRHPCSSSSRRLLPFCSFSEKGEGSSGSLPRGVGEALNGVSQQKVLQVVLVS 143

Query: 125 PLIPGNTGSIARTCAASAVGLHLVGPLGFKVDDTKLKRAGLDYWPYVVVKIHSTWAEFRD 184
           PLIPGNTGSIARTCAASAVGLHLVGPLGFKVDDTKLKRAGLDYWPYVVVKIH+TWAEFRD
Sbjct: 144 PLIPGNTGSIARTCAASAVGLHLVGPLGFKVDDTKLKRAGLDYWPYVVVKIHNTWAEFRD 203

Query: 185 YFNKQ------------------DFSYKRGDYLLFGSETSGLPPEALEDCKNQPFGGGTI 244
           YF KQ                  DFSYK+GDYLLF SETSGLP EALEDCKNQ FGGGT+
Sbjct: 204 YFKKQEGEKRLLAFTKRGTTLHSDFSYKQGDYLLFRSETSGLPAEALEDCKNQAFGGGTL 263

Query: 245 RIPMVETYVRCLNLSVSVGIAIYEASRQLNYEQLQFAPEGCT-NNDQSIITEDLFA 273
           RIPMVETYVRCLNLSVSVGIAIYEASRQLNY+QLQ  PEGCT NN+QSIITEDLFA
Sbjct: 264 RIPMVETYVRCLNLSVSVGIAIYEASRQLNYQQLQIEPEGCTNNNEQSIITEDLFA 319

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BLAST of Spg013678 vs. ExPASy Swiss-Prot
Match: P74516 (Putative tRNA (cytidine(34)-2'-O)-methyltransferase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=slr0992 PE=3 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 2.7e-29
Identity = 71/155 (45.81%), Postives = 97/155 (62.58%), Query Frame = 0


Query: 108 LLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKVDDTKLKRAGLDYWPYVVVKIH 167
           ++++VLV+P IP NTG+IARTCAA+   LHLVGPLGF++ D  LKRAGLDYWPYV +  H
Sbjct: 1   MVKLVLVNPQIPPNTGNIARTCAATGTELHLVGPLGFELGDRYLKRAGLDYWPYVDLHYH 60

Query: 168 STWAEFRDYFNKQ----------------DFSYKRGDYLLFGSETSGLPPEALEDCKNQP 227
           ++  EF  ++  Q                D++Y+  D+L+ GSET GL P+ + D   Q 
Sbjct: 61  NSCEEFITHWRGQGGNLLGFSVTGTVNFWDYTYQSTDWLMMGSETDGL-PQMMRDLSTQ- 120

Query: 228 FGGGTIRIPMVETYVRCLNLSVSVGIAIYEASRQL 247
                + IPM  + VR LNL+ S  IA++EA RQL
Sbjct: 121 ----LLCIPMPHSEVRSLNLASSAAIALFEAGRQL 149

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BLAST of Spg013678 vs. ExPASy Swiss-Prot
Match: O31590 (Putative tRNA (cytidine(34)-2'-O)-methyltransferase OS=Bacillus subtilis (strain 168) OX=224308 GN=cspR PE=3 SV=2)

HSP 1 Score: 120.2 bits (300), Expect = 3.7e-26
Identity = 70/161 (43.48%), Postives = 92/161 (57.14%), Query Frame = 0


Query: 109 LQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKVDDTKLKRAGLDYWPYVVVKIHS 168
           L VVL  P IP NTG+IARTCAA+   LHL+ PLGF  DD  LKRAGLDYW +V V  H 
Sbjct: 6   LHVVLYQPEIPANTGNIARTCAATNTTLHLIRPLGFSTDDKMLKRAGLDYWEFVNVVYHD 65

Query: 169 TWAEFRDYFNKQDFSY--KRGD---------------YLLFGSETSGLPPEALEDCKNQP 228
           +  E  + + K  F +  K G                + +FG ETSGLP + +++  ++ 
Sbjct: 66  SLEELFEAYKKGKFFFITKFGQQPHTSFDYTDLDEDYFFVFGRETSGLPKDLIQNNMDR- 125

Query: 229 FGGGTIRIPMVETYVRCLNLSVSVGIAIYEASRQLNYEQLQ 253
                +R+PM E +VR LNLS +  I +YEA RQ NY  L+
Sbjct: 126 ----CLRLPMTE-HVRSLNLSNTAAILVYEALRQQNYRDLK 160

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BLAST of Spg013678 vs. ExPASy Swiss-Prot
Match: P32813 (Putative tRNA (cytidine(34)-2'-O)-methyltransferase OS=Geobacillus stearothermophilus OX=1422 PE=3 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 9.1e-25
Identity = 67/160 (41.88%), Postives = 90/160 (56.25%), Query Frame = 0


Query: 109 LQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKVDDTKLKRAGLDYWPYVVVKIHS 168
           L VVL  P IP NTG+IARTCAA+   LHL+ PLGF  DD  L+RAGLDYW +V +  + 
Sbjct: 3   LHVVLFQPEIPANTGNIARTCAATNTALHLIRPLGFSTDDKMLRRAGLDYWEFVNIHYYD 62

Query: 169 TWAEF----------------RDYFNKQDFSYKRGD-YLLFGSETSGLPPEALEDCKNQP 228
           +   F                + Y+   DFS    + + +FG ET+GLP E L + +++ 
Sbjct: 63  SLDHFFQQNQNGDFYYITKFGKRYYTSYDFSDPSKEIFFIFGRETTGLPKELLAENEDR- 122

Query: 229 FGGGTIRIPMVETYVRCLNLSVSVGIAIYEASRQLNYEQL 252
                +RIPM E  VR LNLS +  I +YEA RQ ++  L
Sbjct: 123 ----CLRIPMTEN-VRALNLSNTAAILVYEALRQQSFHGL 156

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BLAST of Spg013678 vs. ExPASy Swiss-Prot
Match: A0KAS4 (tRNA (cytidine(34)-2'-O)-methyltransferase OS=Burkholderia cenocepacia (strain HI2424) OX=331272 GN=trmL PE=3 SV=2)

HSP 1 Score: 112.8 bits (281), Expect = 5.9e-24
Identity = 62/158 (39.24%), Postives = 87/158 (55.06%), Query Frame = 0


Query: 108 LLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKVDDTKLKRAGLDYWPYVVVKIH 167
           +  VVLV+P IP NTG++ R CA +   LHL+ PLGF +DD +++RAGLDY  Y  +++H
Sbjct: 1   MFNVVLVAPEIPPNTGNVIRLCANTGAHLHLIEPLGFPLDDARMRRAGLDYHEYAQMRVH 60

Query: 168 STWAEF----------------RDYFNKQDFSYKRGDYLLFGSETSGLPPEALEDCKNQP 227
             W  F                R      D ++  GD+ +FGSET GLP E LE   N+ 
Sbjct: 61  RDWDAFVAAETPDPARMFAFTTRGSGRFHDHAFLSGDWFVFGSETRGLPAELLERFPNEQ 120

Query: 228 FGGGTIRIPMVETYVRCLNLSVSVGIAIYEASRQLNYE 250
                +R+PM     R LNLS +V + ++EA RQ  +E
Sbjct: 121 ----RVRLPM-RPDNRSLNLSNTVAVVVFEAWRQAGFE 153

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BLAST of Spg013678 vs. ExPASy Swiss-Prot
Match: Q0BBL1 (tRNA (cytidine(34)-2'-O)-methyltransferase OS=Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) OX=339670 GN=trmL PE=3 SV=2)

HSP 1 Score: 112.5 bits (280), Expect = 7.7e-24
Identity = 61/158 (38.61%), Postives = 87/158 (55.06%), Query Frame = 0


Query: 108 LLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKVDDTKLKRAGLDYWPYVVVKIH 167
           +  V+LV+P IP NTG++ R CA +   LHL+ PLGF +DD K++RAGLDY  Y  +++H
Sbjct: 1   MFNVILVAPEIPPNTGNVIRLCANTGARLHLIEPLGFPLDDAKMRRAGLDYHEYAEMRVH 60

Query: 168 STWAEF----------------RDYFNKQDFSYKRGDYLLFGSETSGLPPEALEDCKNQP 227
             W  F                R      D ++  GD+ +FGSET GLP + LE   N+ 
Sbjct: 61  RDWDAFVAAEAPDPARMFAFTTRGSGRFHDHAFVPGDWFVFGSETRGLPADVLERFPNEQ 120

Query: 228 FGGGTIRIPMVETYVRCLNLSVSVGIAIYEASRQLNYE 250
                +R+PM     R LNLS +V + ++EA RQ  +E
Sbjct: 121 ----RVRLPM-RPGNRSLNLSNTVAVVVFEAWRQAGFE 153

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BLAST of Spg013678 vs. ExPASy TrEMBL
Match: A0A0A0K6L9 (SpoU_methylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G428850 PE=3 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 2.6e-91
Identity = 169/204 (82.84%), Postives = 179/204 (87.75%), Query Frame = 0


Query: 87  EGSSSLPRGIGEAVNGVSQPKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKV 146
           EGS SLP G+GEAVNGVS PKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKV
Sbjct: 65  EGSGSLPTGVGEAVNGVSHPKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKV 124

Query: 147 DDTKLKRAGLDYWPYVVVKIHSTWAEFRDYFNKQ------------------DFSYKRGD 206
           DDTKLKRAGLDYWPYVVVKIH+TWA+F++YF KQ                  DFSYKRGD
Sbjct: 125 DDTKLKRAGLDYWPYVVVKIHNTWADFQEYFKKQEGEKRLLAFTKRGTTHHSDFSYKRGD 184

Query: 207 YLLFGSETSGLPPEALEDCKNQPFGGGTIRIPMVETYVRCLNLSVSVGIAIYEASRQLNY 266
           YLLFGSET+GLPPEALEDCKNQPFGGGT+RIPMVETYVRCLNLSVSVGIAIYEASRQLNY
Sbjct: 185 YLLFGSETTGLPPEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASRQLNY 244

Query: 267 EQLQFAPEGCTNNDQSIITEDLFA 273
           EQLQ + E CTNN+QSIITED+FA
Sbjct: 245 EQLQVSSESCTNNEQSIITEDVFA 268

[Close]

BLAST of Spg013678 vs. ExPASy TrEMBL
Match: A0A6J1CVM2 (uncharacterized protein LOC111015191 OS=Momordica charantia OX=3673 GN=LOC111015191 PE=3 SV=1)

HSP 1 Score: 343.2 bits (879), Expect = 1.0e-90
Identity = 165/205 (80.49%), Postives = 180/205 (87.80%), Query Frame = 0


Query: 86  GEGSSSLPRGIGEAVNGVSQPKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFK 145
           GEG +SLPRG+GE++NGVS PKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFK
Sbjct: 61  GEGYASLPRGVGESINGVSHPKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFK 120

Query: 146 VDDTKLKRAGLDYWPYVVVKIHSTWAEFRDYFNKQ------------------DFSYKRG 205
           VDDTKLKRAGLDYWPYVV+KIHSTWA+F+DYF KQ                  +FSY+RG
Sbjct: 121 VDDTKLKRAGLDYWPYVVIKIHSTWADFQDYFKKQEGEKRLLAFTKRGTAIHSEFSYRRG 180

Query: 206 DYLLFGSETSGLPPEALEDCKNQPFGGGTIRIPMVETYVRCLNLSVSVGIAIYEASRQLN 265
           DYLLFGSETSGLP EALEDCKNQPFGGGT+RIPMVETYVRCLNLSVSVGIA+YEASRQLN
Sbjct: 181 DYLLFGSETSGLPAEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAVYEASRQLN 240

Query: 266 YEQLQFAPEGCTNNDQSIITEDLFA 273
           YEQLQF  EGC N++QS+ITED+FA
Sbjct: 241 YEQLQFPAEGCNNDEQSVITEDVFA 265

[Close]

BLAST of Spg013678 vs. ExPASy TrEMBL
Match: A0A5D3CFA4 (Putative tRNA (Cytidine(34)-2'-O)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G001000 PE=3 SV=1)

HSP 1 Score: 342.4 bits (877), Expect = 1.7e-90
Identity = 167/204 (81.86%), Postives = 179/204 (87.75%), Query Frame = 0


Query: 87  EGSSSLPRGIGEAVNGVSQPKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKV 146
           EGS SLP G+GEAVNGVS PK+LQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKV
Sbjct: 113 EGSGSLPSGVGEAVNGVSHPKILQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKV 172

Query: 147 DDTKLKRAGLDYWPYVVVKIHSTWAEFRDYFNKQ------------------DFSYKRGD 206
           DDTKLKRAGLDYWPYVVVKIH+TWA+F++YF KQ                  DFSYKRGD
Sbjct: 173 DDTKLKRAGLDYWPYVVVKIHNTWADFQEYFKKQEGEKRLLAFTKRGTTHHSDFSYKRGD 232

Query: 207 YLLFGSETSGLPPEALEDCKNQPFGGGTIRIPMVETYVRCLNLSVSVGIAIYEASRQLNY 266
           YLLFGSET+GLPPEALEDCKNQPFGGGT+RIPMVETYVRCLNLSVSVGIAIYEASRQLNY
Sbjct: 233 YLLFGSETTGLPPEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASRQLNY 292

Query: 267 EQLQFAPEGCTNNDQSIITEDLFA 273
           EQLQ + E CTN++QSIITED+FA
Sbjct: 293 EQLQVSSESCTNDEQSIITEDVFA 316

[Close]

BLAST of Spg013678 vs. ExPASy TrEMBL
Match: A0A1S3CFJ1 (putative tRNA (Cytidine(34)-2'-O)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103499907 PE=3 SV=1)

HSP 1 Score: 342.4 bits (877), Expect = 1.7e-90
Identity = 167/204 (81.86%), Postives = 179/204 (87.75%), Query Frame = 0


Query: 87  EGSSSLPRGIGEAVNGVSQPKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKV 146
           EGS SLP G+GEAVNGVS PK+LQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKV
Sbjct: 65  EGSGSLPSGVGEAVNGVSHPKILQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKV 124

Query: 147 DDTKLKRAGLDYWPYVVVKIHSTWAEFRDYFNKQ------------------DFSYKRGD 206
           DDTKLKRAGLDYWPYVVVKIH+TWA+F++YF KQ                  DFSYKRGD
Sbjct: 125 DDTKLKRAGLDYWPYVVVKIHNTWADFQEYFKKQEGEKRLLAFTKRGTTHHSDFSYKRGD 184

Query: 207 YLLFGSETSGLPPEALEDCKNQPFGGGTIRIPMVETYVRCLNLSVSVGIAIYEASRQLNY 266
           YLLFGSET+GLPPEALEDCKNQPFGGGT+RIPMVETYVRCLNLSVSVGIAIYEASRQLNY
Sbjct: 185 YLLFGSETTGLPPEALEDCKNQPFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASRQLNY 244

Query: 267 EQLQFAPEGCTNNDQSIITEDLFA 273
           EQLQ + E CTN++QSIITED+FA
Sbjct: 245 EQLQVSSESCTNDEQSIITEDVFA 268

[Close]

BLAST of Spg013678 vs. ExPASy TrEMBL
Match: A0A6J1KVT5 (uncharacterized protein LOC111498049 OS=Cucurbita maxima OX=3661 GN=LOC111498049 PE=3 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 3.8e-90
Identity = 195/296 (65.88%), Postives = 216/296 (72.97%), Query Frame = 0


Query: 5   RSKLRQRDTQSIAEERQREEEDFLAKNAIHGHNNNHSQS---FAPIFVEGSSSSSPLLFS 64
           R +   R+ +S AEER+R+EED  AK  +        ++   F+P  +  S+S   L   
Sbjct: 24  RPEQLNREARSTAEERRRQEEDCSAKMPLMDTTATTLRTLHPFSPFLLRSSTSHRLLRAR 83

Query: 65  PFQPQTAPSVFFFLSSAPPLLLLL-----GEGSS-SLPRGIGEAVNGVSQPKLLQVVLVS 124
            F   +        SS+  LL        GEGSS SLPRG+GEA+NGVSQ K+LQVVLVS
Sbjct: 84  CFSHFSHKHRHPCSSSSRRLLPFCSFSEKGEGSSGSLPRGVGEALNGVSQQKVLQVVLVS 143

Query: 125 PLIPGNTGSIARTCAASAVGLHLVGPLGFKVDDTKLKRAGLDYWPYVVVKIHSTWAEFRD 184
           PLIPGNTGSIARTCAASAVGLHLVGPLGFKVDDTKLKRAGLDYWPYVVVKIH+TWAEFRD
Sbjct: 144 PLIPGNTGSIARTCAASAVGLHLVGPLGFKVDDTKLKRAGLDYWPYVVVKIHNTWAEFRD 203

Query: 185 YFNKQ------------------DFSYKRGDYLLFGSETSGLPPEALEDCKNQPFGGGTI 244
           YF KQ                  DFSYK+GDYLLF SETSGLP EALEDCKNQ FGGGT+
Sbjct: 204 YFKKQEGEKRLLAFTKRGTTLHSDFSYKQGDYLLFRSETSGLPAEALEDCKNQAFGGGTL 263

Query: 245 RIPMVETYVRCLNLSVSVGIAIYEASRQLNYEQLQFAPEGCT-NNDQSIITEDLFA 273
           RIPMVETYVRCLNLSVSVGIAIYEASRQLNY+QLQ  PEGCT NN+QSIITEDLFA
Sbjct: 264 RIPMVETYVRCLNLSVSVGIAIYEASRQLNYQQLQIEPEGCTNNNEQSIITEDLFA 319

[Close]

BLAST of Spg013678 vs. TAIR 10
Match: AT5G26880.2 (AGAMOUS-like 26 )

HSP 1 Score: 290.8 bits (743), Expect = 1.1e-78
Identity = 138/199 (69.35%), Postives = 161/199 (80.90%), Query Frame = 0


Query: 92  LPRGIGEAVNGVSQPKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKVDDTKL 151
           LPRG GE V   ++ KLL VVLVSP IPGNTG IARTCAASAVGLHLVGPLGF+VDD ++
Sbjct: 65  LPRGAGEGVKEDARSKLLHVVLVSPQIPGNTGCIARTCAASAVGLHLVGPLGFQVDDARV 124

Query: 152 KRAGLDYWPYVVVKIHSTWAEFRDYFNKQ------------------DFSYKRGDYLLFG 211
           KRAGLDYWP+VVVK HS+WAEF++YF  Q                  DFSY+ GDYLLFG
Sbjct: 125 KRAGLDYWPFVVVKAHSSWAEFQEYFRLQEGEKRMIAFTKRGTRIHSDFSYRSGDYLLFG 184

Query: 212 SETSGLPPEALEDCKNQPFGGGTIRIPMVETYVRCLNLSVSVGIAIYEASRQLNYEQLQF 271
           SETSGLPPEAL DC ++P+GGGT+RIPMVETYVRCLNLSVSVGIA+YEASRQLNYEQ++ 
Sbjct: 185 SETSGLPPEALSDCNHEPYGGGTLRIPMVETYVRCLNLSVSVGIALYEASRQLNYEQIEC 244

Query: 272 APEGCTNNDQSIITEDLFA 273
           AP+GC N ++ ++TED+FA
Sbjct: 245 APQGCVNGEEPLLTEDIFA 263

[Close]

BLAST of Spg013678 vs. TAIR 10
Match: AT5G26880.1 (AGAMOUS-like 26 )

HSP 1 Score: 231.5 bits (589), Expect = 8.1e-61
Identity = 111/160 (69.38%), Postives = 126/160 (78.75%), Query Frame = 0


Query: 92  LPRGIGEAVNGVSQPKLLQVVLVSPLIPGNTGSIARTCAASAVGLHLVGPLGFKVDDTKL 151
           LPRG GE V   ++ KLL VVLVSP IPGNTG IARTCAASAVGLHLVGPLGF+VDD ++
Sbjct: 65  LPRGAGEGVKEDARSKLLHVVLVSPQIPGNTGCIARTCAASAVGLHLVGPLGFQVDDARV 124

Query: 152 KRAGLDYWPYVVVKIHSTWAEFRDYFNKQ------------------DFSYKRGDYLLFG 211
           KRAGLDYWP+VVVK HS+WAEF++YF  Q                  DFSY+ GDYLLFG
Sbjct: 125 KRAGLDYWPFVVVKAHSSWAEFQEYFRLQEGEKRMIAFTKRGTRIHSDFSYRSGDYLLFG 184

Query: 212 SETSGLPPEALEDCKNQPFGGGTIRIPMVETYVRCLNLSV 234
           SETSGLPPEAL DC ++P+GGGT+RIPMVETYVRCLNLS+
Sbjct: 185 SETSGLPPEALSDCNHEPYGGGTLRIPMVETYVRCLNLSI 224

The following BLAST results are available for this feature:

BLAST of Spg013678 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004135918.1	5.4e-91	82.84	uncharacterized protein LOC101203702 [Cucumis sativus] >KGN45148.1 hypothetical ...	[more]
XP_022145830.1	2.1e-90	80.49	uncharacterized protein LOC111015191 [Momordica charantia]	[more]
KAA0058644.1	3.5e-90	81.86	putative tRNA (cytidine(34)-2'-O)-methyltransferase [Cucumis melo var. makuwa] >...	[more]
XP_008461270.1	3.5e-90	81.86	PREDICTED: putative tRNA (cytidine(34)-2'-O)-methyltransferase [Cucumis melo]	[more]
XP_023004870.1	7.8e-90	65.88	uncharacterized protein LOC111498049 [Cucurbita maxima]	[more]

BLAST of Spg013678 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P74516	2.7e-29	45.81	Putative tRNA (cytidine(34)-2'-O)-methyltransferase OS=Synechocystis sp. (strain...	[more]
O31590	3.7e-26	43.48	Putative tRNA (cytidine(34)-2'-O)-methyltransferase OS=Bacillus subtilis (strain...	[more]
P32813	9.1e-25	41.88	Putative tRNA (cytidine(34)-2'-O)-methyltransferase OS=Geobacillus stearothermop...	[more]
A0KAS4	5.9e-24	39.24	tRNA (cytidine(34)-2'-O)-methyltransferase OS=Burkholderia cenocepacia (strain H...	[more]
Q0BBL1	7.7e-24	38.61	tRNA (cytidine(34)-2'-O)-methyltransferase OS=Burkholderia ambifaria (strain ATC...	[more]

BLAST of Spg013678 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K6L9	2.6e-91	82.84	SpoU_methylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G428...	[more]
A0A6J1CVM2	1.0e-90	80.49	uncharacterized protein LOC111015191 OS=Momordica charantia OX=3673 GN=LOC111015...	[more]
A0A5D3CFA4	1.7e-90	81.86	Putative tRNA (Cytidine(34)-2'-O)-methyltransferase OS=Cucumis melo var. makuwa ...	[more]
A0A1S3CFJ1	1.7e-90	81.86	putative tRNA (Cytidine(34)-2'-O)-methyltransferase OS=Cucumis melo OX=3656 GN=L...	[more]
A0A6J1KVT5	3.8e-90	65.88	uncharacterized protein LOC111498049 OS=Cucurbita maxima OX=3661 GN=LOC111498049...	[more]

BLAST of Spg013678 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G26880.2	1.1e-78	69.35	AGAMOUS-like 26	[more]
AT5G26880.1	8.1e-61	69.38	AGAMOUS-like 26	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029026	tRNA (guanine-N1-)-methyltransferase, N-terminal	GENE3D	3.40.1280.10		coord: 98..260 e-value: 1.3E-34 score: 121.1
IPR001537	tRNA/rRNA methyltransferase, SpoU type	PFAM	PF00588	SpoU_methylase	coord: 109..240 e-value: 1.3E-23 score: 83.8
None	No IPR available	PIRSR	PIRSR029256-1	PIRSR029256-1	coord: 109..248 e-value: 3.0E-39 score: 131.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..22
IPR016914	tRNA (cytidine/uridine-2'-O-)-methyltransferase	PANTHER	PTHR42971	TRNA (CYTIDINE(34)-2'-O)-METHYLTRANSFERASE	coord: 102..270
IPR016914	tRNA (cytidine/uridine-2'-O-)-methyltransferase	HAMAP	MF_01885	tRNA_methyltr_TrmL	coord: 109..249 score: 28.725311
IPR016914	tRNA (cytidine/uridine-2'-O-)-methyltransferase	CDD	cd18094	SpoU-like_TrmL	coord: 110..246 e-value: 1.35749E-70 score: 212.313
IPR029028	Alpha/beta knot methyltransferases	SUPERFAMILY	75217	alpha/beta knot	coord: 109..251

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg013678.1	Spg013678.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0002131	wobble position cytosine ribose methylation
biological_process	GO:0002132	wobble position uridine ribose methylation
biological_process	GO:0001510	RNA methylation
biological_process	GO:0006396	RNA processing
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0008173	RNA methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity