Spg013562 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg013562
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionAuxin-responsive GH3 family protein
Locationscaffold1: 8263893 .. 8266437 (-)
RNA-Seq ExpressionSpg013562
SyntenySpg013562
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCACCGCTCCATTATAACATTACTGCAATCTCTCTCTCTTTCTCTTTAAATTCTTGCTCTCTTCGTAATCTAAAATTAATGGCCGTTGATTCTCAGCTTTCCTCTCCTCTTGGGCCGCCGGCATGCGACAAGGATGCCAAAGCCCTTCGTTTCATACACGACACCACTACAAATGCCGACTCGGTTCAGCAGAGGGTCTTGGCCGAGATTCTGACCCGGAACGCCCACACTGAGTACGTGACCCGCTTCCGCCTCAATGGAGCCACCGACCGGGACACCTTTAAGTCCAAACTCCCGGTGGTTACTTACGAGGATCTCCAGCCCGACATCCAACGCATCGCTAATGGCGATCGCTCTCCTATTTTTTCATCCCATCCCATTTCTGAGTTTCTTACCAGGTGACAACTTTTAATCTCAACATATACAAATTAATTAAGCTACAGTATGTTAAGGTCGATTTTATTCAGATTTATAAATGGGGTTTTTATAATTTGCAGTTCTGGGACATCGGCTGGGGAGAGAAAACTGATGCCCACAATTAAAGAAGAAATGGAACGTCGTCAGTTGCTTTACAGCCTTCTCATGCCCATCATGAATCTGTAAGAATATAATTCACTCTCTATTCAATTTCGATTTCAATTCAGTCACTTTTTTAATTCAAGACAAGAATTTCATTTTGACGAATTCACAAAATTTTCAGGTACGTGCCGGGGTTGGACAAAGGGAAAGGGCTCTACTTTTTATTCGTGAAGGCCGAAACCAAGACCCCTGGAGGGCTGGTGGCTCGACCAGTGCTCACCAGCTACTACAAGAGCGACATCTTCAAGACTAGACCCTACGACCCTTTCAATGACTACACCAGCCCAAACGAGGCCATTCTTTGCGCCGATTCCTTCCAAAGCATGTACACTCAGATGCTATGTGGCCTCTTGATGCGCGGCCAAGTCCTCCGGGTCGGCGCCGTCTTCGCCTCCGGCCTCCTCCGTGCCATTCACTTCCTCCAGCTCAACTGGAAGCAGCTTGCACACGACATCTCCACCGGAACCCTTAACCCCAAAATCACTGACCCTTCTCTCCGTGAATGCATAATCTCCAAATACTTGACAAACCCAAATCCCGAGCTGGCCGAATTCATTTCCAAGGAGTGCTCCACAGAGGAATGGGAAGGAATCGTCACACGAATCTGGCCAAACACAAAATACCTGGATGTGATCGTGACAGGAGCGATGGCTCAGTACATTCCGACGCTGGAATTTTACAGCGGGGGATTACCCATGGCTTGCACCATGTACGCTTCCTCGGAGTGCTATTTCGGGGTTAATTTGAAGCCAATGTGCAAACCCTCGGAAGTAACCTACACCATCATGCCCAACATGGGCTACTTCGAATTCCTCCCGCACGACACGTCGTCCCCTCCAGCCCTCTCCCGCGACTCCCCGCCTCGACTCGTCGACCTCGCTGACGTGGAAGTCGGGAAAGAGTACGAGCTCGTGATCACAACCTACTCCGGACTGTGCCGCTACCGCGTCGGCGACATCCTACAGGTAACTGGGTTCCACAACGCGGCCCCACAGTTCCGATTCGTGAGGCGAAAGAACGTTCTGCTGAGCATCGATTCCGACAAGACCGACGAGGCGGAGCTCCAGAAGGCAATCGAGAACGCTTCATCTCTCCTGCGAGAGTTCAACACGAGCGTGGTGGAATACACGAGCTACGCAGACACCAAAACAATCCCGGGACATTACGTCATATATTGGGAGCTTCGTGTCAAGGACGCTGCAAATTCCCCGCCGGAGGAGATTCTAAACCAGTGCTGCCTGGCCATGGAAGAGAGCCTGAACTCGGTGTACCGCCAAGGCCGGGTGGCGGATAACTCGATCGGACCGCTGGAGATCAGGGTGGTGAAAAACGGGACGTTTGAAGAGCTAATGGACTACGCGATCTCAAGGGGCGCGTCGATCAATCAGTACAAAGCGCCACGCTGCGTAAACTTCACACCCATTGTCGAACTCCTCGACTCTAGGGTAACTTCAGTGCACTTCAGCCCATCGAAGCCGCACTGGACGCCGGAACGACGCCGTTGAAATCAACCAACAAAAATAATATAAATAAAAATTCACATTAAAGGGAAAAAAGAAAAAAAGGAAAAAGACCCATATGTATAAAAGATTGTATGTGATGATGATGTAGCAGCCAAGCAGAAAAGGATAGAGTTTGAGTTTGTTGGATTATGCATATGGCTTTTAGAAGTGTCTTTGATTTTAGGGTTTTTTTTTTTTTTTTTTTTGGGGGGGGTTAGTTGATTTAGTGTCTGTGTAATAAGGTTCTGAGTAATGTCTAAAATATGGGCTTCATGATGTAATTGTTCTTTTACATATGTGTCTGTTGGTCTATTGCATTATCTTTTTGTCTCTGGCTTTCTTTCTGTTTTTCCTTTCCTTTTGAGAGTAAAGAGAGAGTGGAAAAGAAAAGAATAATAACAAAAAAGCAAAGAAAAGTTGTTGAAATTAAAGTGTGTGTTGTTTTAATAAATATGGGTTTCAT

mRNA sequence

ATGGCCGTTGATTCTCAGCTTTCCTCTCCTCTTGGGCCGCCGGCATGCGACAAGGATGCCAAAGCCCTTCGTTTCATACACGACACCACTACAAATGCCGACTCGGTTCAGCAGAGGGTCTTGGCCGAGATTCTGACCCGGAACGCCCACACTGAGTACGTGACCCGCTTCCGCCTCAATGGAGCCACCGACCGGGACACCTTTAAGTCCAAACTCCCGGTGGTTACTTACGAGGATCTCCAGCCCGACATCCAACGCATCGCTAATGGCGATCGCTCTCCTATTTTTTCATCCCATCCCATTTCTGAGTTTCTTACCAGTTCTGGGACATCGGCTGGGGAGAGAAAACTGATGCCCACAATTAAAGAAGAAATGGAACGTCGTCAGTTGCTTTACAGCCTTCTCATGCCCATCATGAATCTGTACGTGCCGGGGTTGGACAAAGGGAAAGGGCTCTACTTTTTATTCGTGAAGGCCGAAACCAAGACCCCTGGAGGGCTGGTGGCTCGACCAGTGCTCACCAGCTACTACAAGAGCGACATCTTCAAGACTAGACCCTACGACCCTTTCAATGACTACACCAGCCCAAACGAGGCCATTCTTTGCGCCGATTCCTTCCAAAGCATGTACACTCAGATGCTATGTGGCCTCTTGATGCGCGGCCAAGTCCTCCGGGTCGGCGCCGTCTTCGCCTCCGGCCTCCTCCGTGCCATTCACTTCCTCCAGCTCAACTGGAAGCAGCTTGCACACGACATCTCCACCGGAACCCTTAACCCCAAAATCACTGACCCTTCTCTCCGTGAATGCATAATCTCCAAATACTTGACAAACCCAAATCCCGAGCTGGCCGAATTCATTTCCAAGGAGTGCTCCACAGAGGAATGGGAAGGAATCGTCACACGAATCTGGCCAAACACAAAATACCTGGATGTGATCGTGACAGGAGCGATGGCTCAGTACATTCCGACGCTGGAATTTTACAGCGGGGGATTACCCATGGCTTGCACCATGTACGCTTCCTCGGAGTGCTATTTCGGGGTTAATTTGAAGCCAATGTGCAAACCCTCGGAAGTAACCTACACCATCATGCCCAACATGGGCTACTTCGAATTCCTCCCGCACGACACGTCGTCCCCTCCAGCCCTCTCCCGCGACTCCCCGCCTCGACTCGTCGACCTCGCTGACGTGGAAGTCGGGAAAGAGTACGAGCTCGTGATCACAACCTACTCCGGACTGTGCCGCTACCGCGTCGGCGACATCCTACAGGTAACTGGGTTCCACAACGCGGCCCCACAGTTCCGATTCGTGAGGCGAAAGAACGTTCTGCTGAGCATCGATTCCGACAAGACCGACGAGGCGGAGCTCCAGAAGGCAATCGAGAACGCTTCATCTCTCCTGCGAGAGTTCAACACGAGCGTGGTGGAATACACGAGCTACGCAGACACCAAAACAATCCCGGGACATTACGTCATATATTGGGAGCTTCGTGTCAAGGACGCTGCAAATTCCCCGCCGGAGGAGATTCTAAACCAGTGCTGCCTGGCCATGGAAGAGAGCCTGAACTCGGTGTACCGCCAAGGCCGGGTGGCGGATAACTCGATCGGACCGCTGGAGATCAGGGTGGTGAAAAACGGGACGTTTGAAGAGCTAATGGACTACGCGATCTCAAGGGGCGCGTCGATCAATCAGTACAAAGCGCCACGCTGCGTAAACTTCACACCCATTGTCGAACTCCTCGACTCTAGGGTAACTTCAGTGCACTTCAGCCCATCGAAGCCGCACTGGACGCCGGAACGACGCCGTTGA

Coding sequence (CDS)

ATGGCCGTTGATTCTCAGCTTTCCTCTCCTCTTGGGCCGCCGGCATGCGACAAGGATGCCAAAGCCCTTCGTTTCATACACGACACCACTACAAATGCCGACTCGGTTCAGCAGAGGGTCTTGGCCGAGATTCTGACCCGGAACGCCCACACTGAGTACGTGACCCGCTTCCGCCTCAATGGAGCCACCGACCGGGACACCTTTAAGTCCAAACTCCCGGTGGTTACTTACGAGGATCTCCAGCCCGACATCCAACGCATCGCTAATGGCGATCGCTCTCCTATTTTTTCATCCCATCCCATTTCTGAGTTTCTTACCAGTTCTGGGACATCGGCTGGGGAGAGAAAACTGATGCCCACAATTAAAGAAGAAATGGAACGTCGTCAGTTGCTTTACAGCCTTCTCATGCCCATCATGAATCTGTACGTGCCGGGGTTGGACAAAGGGAAAGGGCTCTACTTTTTATTCGTGAAGGCCGAAACCAAGACCCCTGGAGGGCTGGTGGCTCGACCAGTGCTCACCAGCTACTACAAGAGCGACATCTTCAAGACTAGACCCTACGACCCTTTCAATGACTACACCAGCCCAAACGAGGCCATTCTTTGCGCCGATTCCTTCCAAAGCATGTACACTCAGATGCTATGTGGCCTCTTGATGCGCGGCCAAGTCCTCCGGGTCGGCGCCGTCTTCGCCTCCGGCCTCCTCCGTGCCATTCACTTCCTCCAGCTCAACTGGAAGCAGCTTGCACACGACATCTCCACCGGAACCCTTAACCCCAAAATCACTGACCCTTCTCTCCGTGAATGCATAATCTCCAAATACTTGACAAACCCAAATCCCGAGCTGGCCGAATTCATTTCCAAGGAGTGCTCCACAGAGGAATGGGAAGGAATCGTCACACGAATCTGGCCAAACACAAAATACCTGGATGTGATCGTGACAGGAGCGATGGCTCAGTACATTCCGACGCTGGAATTTTACAGCGGGGGATTACCCATGGCTTGCACCATGTACGCTTCCTCGGAGTGCTATTTCGGGGTTAATTTGAAGCCAATGTGCAAACCCTCGGAAGTAACCTACACCATCATGCCCAACATGGGCTACTTCGAATTCCTCCCGCACGACACGTCGTCCCCTCCAGCCCTCTCCCGCGACTCCCCGCCTCGACTCGTCGACCTCGCTGACGTGGAAGTCGGGAAAGAGTACGAGCTCGTGATCACAACCTACTCCGGACTGTGCCGCTACCGCGTCGGCGACATCCTACAGGTAACTGGGTTCCACAACGCGGCCCCACAGTTCCGATTCGTGAGGCGAAAGAACGTTCTGCTGAGCATCGATTCCGACAAGACCGACGAGGCGGAGCTCCAGAAGGCAATCGAGAACGCTTCATCTCTCCTGCGAGAGTTCAACACGAGCGTGGTGGAATACACGAGCTACGCAGACACCAAAACAATCCCGGGACATTACGTCATATATTGGGAGCTTCGTGTCAAGGACGCTGCAAATTCCCCGCCGGAGGAGATTCTAAACCAGTGCTGCCTGGCCATGGAAGAGAGCCTGAACTCGGTGTACCGCCAAGGCCGGGTGGCGGATAACTCGATCGGACCGCTGGAGATCAGGGTGGTGAAAAACGGGACGTTTGAAGAGCTAATGGACTACGCGATCTCAAGGGGCGCGTCGATCAATCAGTACAAAGCGCCACGCTGCGTAAACTTCACACCCATTGTCGAACTCCTCGACTCTAGGGTAACTTCAGTGCACTTCAGCCCATCGAAGCCGCACTGGACGCCGGAACGACGCCGTTGA

Protein sequence

MAVDSQLSSPLGPPACDKDAKALRFIHDTTTNADSVQQRVLAEILTRNAHTEYVTRFRLNGATDRDTFKSKLPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFKTRPYDPFNDYTSPNEAILCADSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHFLQLNWKQLAHDISTGTLNPKITDPSLRECIISKYLTNPNPELAEFISKECSTEEWEGIVTRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTYTIMPNMGYFEFLPHDTSSPPALSRDSPPRLVDLADVEVGKEYELVITTYSGLCRYRVGDILQVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAIENASSLLREFNTSVVEYTSYADTKTIPGHYVIYWELRVKDAANSPPEEILNQCCLAMEESLNSVYRQGRVADNSIGPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPERRR
Homology
BLAST of Spg013562 vs. NCBI nr
Match: XP_023543141.1 (probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1150.6 bits (2975), Expect = 0.0e+00
Identity = 568/603 (94.20%), Postives = 584/603 (96.85%), Query Frame = 0

Query: 1   MAVDSQLSSPLGPPACDKDAKALRFIHDTTTNADSVQQRVLAEILTRNAHTEYVTRFRLN 60
           MAVDSQLSSPL PP  DKDAKALRFI + TTN D+VQQRVLAEIL+RNA+TEYV RFRLN
Sbjct: 1   MAVDSQLSSPLPPPPSDKDAKALRFIQEVTTNTDAVQQRVLAEILSRNANTEYVNRFRLN 60

Query: 61  GATDRDTFKSKLPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPT 120
           GATDRDT+KSK+PVVTYEDLQPDIQRIANGDRSPIFSS PISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDRDTYKSKIPVVTYEDLQPDIQRIANGDRSPIFSSTPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD 180
           I+EEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD
Sbjct: 121 IQEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD 180

Query: 181 IFKTRPYDPFNDYTSPNEAILCADSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHF 240
           IFKTRPYDPFNDYTSPNEA+LCADSFQSMYTQMLCGLLMR QVLRVGAVFASGLLRAI F
Sbjct: 181 IFKTRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIRF 240

Query: 241 LQLNWKQLAHDISTGTLNPKITDPSLRECIISKYLTNPNPELAEFISKECSTEEWEGIVT 300
           LQLNWKQLAHDISTGTLNPKITDP LRECI+SKYLTNPNPE+AEFISKECSTEEWEGI+T
Sbjct: 241 LQLNWKQLAHDISTGTLNPKITDPCLRECIVSKYLTNPNPEVAEFISKECSTEEWEGIIT 300

Query: 301 RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTY 360
           RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTY
Sbjct: 301 RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTY 360

Query: 361 TIMPNMGYFEFLPHDTSSPPALSRDSPPRLVDLADVEVGKEYELVITTYSGLCRYRVGDI 420
           TIMPNMGYFEFLPHDTSSPP LSRDSPPRLVDLADVEVGKEYELVITTY+GL RYRVGD+
Sbjct: 361 TIMPNMGYFEFLPHDTSSPPVLSRDSPPRLVDLADVEVGKEYELVITTYAGLRRYRVGDV 420

Query: 421 LQVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAIENASSLLREFNTSVVEYTSYA 480
           L+VTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKA+ENASSLLRE NTSVVEYTSYA
Sbjct: 421 LEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLRELNTSVVEYTSYA 480

Query: 481 DTKTIPGHYVIYWELRVKD-AANS-PPEEILNQCCLAMEESLNSVYRQGRVADNSIGPLE 540
           DTKTIPGHYVIYWEL VKD AANS PP EILNQCCLA+EESLNSVYRQGRVADNSIGPLE
Sbjct: 481 DTKTIPGHYVIYWELLVKDEAANSPPPAEILNQCCLAIEESLNSVYRQGRVADNSIGPLE 540

Query: 541 IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPE 600
           IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRV SVHFSPSKPHWTPE
Sbjct: 541 IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVASVHFSPSKPHWTPE 600

Query: 601 RRR 602
           RRR
Sbjct: 601 RRR 603

BLAST of Spg013562 vs. NCBI nr
Match: KAG6604151.1 (putative indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1149.4 bits (2972), Expect = 0.0e+00
Identity = 567/603 (94.03%), Postives = 584/603 (96.85%), Query Frame = 0

Query: 1   MAVDSQLSSPLGPPACDKDAKALRFIHDTTTNADSVQQRVLAEILTRNAHTEYVTRFRLN 60
           MAVDSQLSSPL PP  DKDAKALRFI + TTN D+VQQRVLAEIL+RNA+TEYV RFRLN
Sbjct: 1   MAVDSQLSSPLPPPPSDKDAKALRFIEEVTTNTDAVQQRVLAEILSRNANTEYVNRFRLN 60

Query: 61  GATDRDTFKSKLPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPT 120
           GATDRDT+KSK+PVVTYEDLQPDIQRIANGDRSPIFSS PISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDRDTYKSKIPVVTYEDLQPDIQRIANGDRSPIFSSTPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD 180
           I+EEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD
Sbjct: 121 IQEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD 180

Query: 181 IFKTRPYDPFNDYTSPNEAILCADSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHF 240
           IFKTRPYDPFNDYTSPNEA+LCADSFQSMYTQMLCGLLMR QVLRVGAVFASGLLRAI F
Sbjct: 181 IFKTRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIRF 240

Query: 241 LQLNWKQLAHDISTGTLNPKITDPSLRECIISKYLTNPNPELAEFISKECSTEEWEGIVT 300
           LQLNWKQLAHDISTGTLNPKITDP LRECI+SKYLTNPNPE+AEFISKECSTEEWEGI+T
Sbjct: 241 LQLNWKQLAHDISTGTLNPKITDPCLRECIVSKYLTNPNPEVAEFISKECSTEEWEGIIT 300

Query: 301 RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTY 360
           RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTY
Sbjct: 301 RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTY 360

Query: 361 TIMPNMGYFEFLPHDTSSPPALSRDSPPRLVDLADVEVGKEYELVITTYSGLCRYRVGDI 420
           TIMPNMGYFEFLPHDTSSPP LSRDSPPRLVDLADVEVGKEYELVITTY+GL RYRVGD+
Sbjct: 361 TIMPNMGYFEFLPHDTSSPPVLSRDSPPRLVDLADVEVGKEYELVITTYAGLRRYRVGDV 420

Query: 421 LQVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAIENASSLLREFNTSVVEYTSYA 480
           L+VTGFHNAAPQFRFVRRKNVLLSID+DKTDEAELQKA+ENASSLLRE NTSVVEYTSYA
Sbjct: 421 LEVTGFHNAAPQFRFVRRKNVLLSIDADKTDEAELQKAVENASSLLRELNTSVVEYTSYA 480

Query: 481 DTKTIPGHYVIYWELRVKD-AANS-PPEEILNQCCLAMEESLNSVYRQGRVADNSIGPLE 540
           DTKTIPGHYVIYWEL VKD AANS PP EILNQCCLA+EESLNSVYRQGRVADNSIGPLE
Sbjct: 481 DTKTIPGHYVIYWELLVKDEAANSPPPAEILNQCCLAIEESLNSVYRQGRVADNSIGPLE 540

Query: 541 IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPE 600
           IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRV SVHFSPSKPHWTPE
Sbjct: 541 IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVASVHFSPSKPHWTPE 600

Query: 601 RRR 602
           RRR
Sbjct: 601 RRR 603

BLAST of Spg013562 vs. NCBI nr
Match: XP_022950930.1 (probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita moschata])

HSP 1 Score: 1146.3 bits (2964), Expect = 0.0e+00
Identity = 566/603 (93.86%), Postives = 583/603 (96.68%), Query Frame = 0

Query: 1   MAVDSQLSSPLGPPACDKDAKALRFIHDTTTNADSVQQRVLAEILTRNAHTEYVTRFRLN 60
           MAVDSQLSSPL PP  DKDAKALRFI + TTN D+VQQRVLAEIL+RNA+TEYV RFRLN
Sbjct: 1   MAVDSQLSSPLPPPPSDKDAKALRFIEEVTTNTDAVQQRVLAEILSRNANTEYVNRFRLN 60

Query: 61  GATDRDTFKSKLPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPT 120
           GATDRDT+KSK+PVVTYEDLQPDIQRIANGDRSPIFSS PISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDRDTYKSKIPVVTYEDLQPDIQRIANGDRSPIFSSTPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD 180
           I+EEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD
Sbjct: 121 IQEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD 180

Query: 181 IFKTRPYDPFNDYTSPNEAILCADSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHF 240
           IFKTRPYDPFNDYTSPNEA+LCADSFQSMYTQMLCGLLMR QVLRVGAVFASGLLRAI F
Sbjct: 181 IFKTRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIRF 240

Query: 241 LQLNWKQLAHDISTGTLNPKITDPSLRECIISKYLTNPNPELAEFISKECSTEEWEGIVT 300
           LQLNWKQLAHDISTGTLNPKITDP LRECI+SKYLTN NPE+AEFISKECSTEEWEGI+T
Sbjct: 241 LQLNWKQLAHDISTGTLNPKITDPCLRECIVSKYLTNTNPEVAEFISKECSTEEWEGIIT 300

Query: 301 RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTY 360
           RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTY
Sbjct: 301 RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTY 360

Query: 361 TIMPNMGYFEFLPHDTSSPPALSRDSPPRLVDLADVEVGKEYELVITTYSGLCRYRVGDI 420
           TIMPNMGYFEFLPHDTSSPP LSRDSPPRLVDLADVEVGKEYELVITTY+GL RYRVGD+
Sbjct: 361 TIMPNMGYFEFLPHDTSSPPVLSRDSPPRLVDLADVEVGKEYELVITTYAGLRRYRVGDV 420

Query: 421 LQVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAIENASSLLREFNTSVVEYTSYA 480
           L+VTGFHNAAPQFRFVRRKNVLLSID+DKTDEAELQKA+ENASSLLRE NTSVVEYTSYA
Sbjct: 421 LEVTGFHNAAPQFRFVRRKNVLLSIDADKTDEAELQKAVENASSLLRELNTSVVEYTSYA 480

Query: 481 DTKTIPGHYVIYWELRVKD-AANS-PPEEILNQCCLAMEESLNSVYRQGRVADNSIGPLE 540
           DTKTIPGHYVIYWEL VKD AANS PP EILNQCCLA+EESLNSVYRQGRVADNSIGPLE
Sbjct: 481 DTKTIPGHYVIYWELLVKDEAANSPPPAEILNQCCLAIEESLNSVYRQGRVADNSIGPLE 540

Query: 541 IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPE 600
           IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRV SVHFSPSKPHWTPE
Sbjct: 541 IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVASVHFSPSKPHWTPE 600

Query: 601 RRR 602
           RRR
Sbjct: 601 RRR 603

BLAST of Spg013562 vs. NCBI nr
Match: KAG7034313.1 (putative indole-3-acetic acid-amido synthetase GH3.1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1143.6 bits (2957), Expect = 0.0e+00
Identity = 563/601 (93.68%), Postives = 582/601 (96.84%), Query Frame = 0

Query: 3    VDSQLSSPLGPPACDKDAKALRFIHDTTTNADSVQQRVLAEILTRNAHTEYVTRFRLNGA 62
            +DSQLSSPL PP  DKDAKALRFI + TTN D+VQQRVLAEIL+RNA+TEYV RFRLNGA
Sbjct: 591  LDSQLSSPLPPPPSDKDAKALRFIEEVTTNTDAVQQRVLAEILSRNANTEYVNRFRLNGA 650

Query: 63   TDRDTFKSKLPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIK 122
            TDRDT+KSK+PVVTYEDLQPDIQRIANGDRSPIFSS PISEFLTSSGTSAGERKLMPTI+
Sbjct: 651  TDRDTYKSKIPVVTYEDLQPDIQRIANGDRSPIFSSTPISEFLTSSGTSAGERKLMPTIQ 710

Query: 123  EEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIF 182
            EEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIF
Sbjct: 711  EEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIF 770

Query: 183  KTRPYDPFNDYTSPNEAILCADSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHFLQ 242
            KTRPYDPFNDYTSPNEA+LCADSFQSMYTQMLCGLLMR QVLRVGAVFASGLLRAI FLQ
Sbjct: 771  KTRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIRFLQ 830

Query: 243  LNWKQLAHDISTGTLNPKITDPSLRECIISKYLTNPNPELAEFISKECSTEEWEGIVTRI 302
            LNWKQLAHDISTGTLNPKITDP LRECI++KYLTNPNPE+AEFISKECSTEEWEGI+TRI
Sbjct: 831  LNWKQLAHDISTGTLNPKITDPCLRECIVTKYLTNPNPEVAEFISKECSTEEWEGIITRI 890

Query: 303  WPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTYTI 362
            WPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTYTI
Sbjct: 891  WPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTYTI 950

Query: 363  MPNMGYFEFLPHDTSSPPALSRDSPPRLVDLADVEVGKEYELVITTYSGLCRYRVGDILQ 422
            MPNMGYFEFLPHDTSSPP LSRDSPPRLVDLADVEVGKEYELVITTY+GL RYRVGD+L+
Sbjct: 951  MPNMGYFEFLPHDTSSPPVLSRDSPPRLVDLADVEVGKEYELVITTYAGLRRYRVGDVLE 1010

Query: 423  VTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAIENASSLLREFNTSVVEYTSYADT 482
            VTGFHNAAPQFRFVRRKNVLLSID+DKTDEAELQKA+ENASSLLRE NTSVVEYTSYADT
Sbjct: 1011 VTGFHNAAPQFRFVRRKNVLLSIDADKTDEAELQKAVENASSLLRELNTSVVEYTSYADT 1070

Query: 483  KTIPGHYVIYWELRVKD-AANS-PPEEILNQCCLAMEESLNSVYRQGRVADNSIGPLEIR 542
            KTIPGHYVIYWEL VKD AANS PP EILNQCCLA+EESLNSVYRQGRVADNSIGPLEIR
Sbjct: 1071 KTIPGHYVIYWELLVKDEAANSPPPAEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIR 1130

Query: 543  VVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPERR 602
            VVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRV SVHFSPSKPHWTPERR
Sbjct: 1131 VVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVASVHFSPSKPHWTPERR 1190

BLAST of Spg013562 vs. NCBI nr
Match: XP_022978925.1 (probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita maxima])

HSP 1 Score: 1143.3 bits (2956), Expect = 0.0e+00
Identity = 564/603 (93.53%), Postives = 583/603 (96.68%), Query Frame = 0

Query: 1   MAVDSQLSSPLGPPACDKDAKALRFIHDTTTNADSVQQRVLAEILTRNAHTEYVTRFRLN 60
           MAV SQLSSPL PP  D+DAKALRFI + TTNAD+VQQRVLAEIL+RNA+TEYV RFRLN
Sbjct: 1   MAVGSQLSSPLPPPPSDRDAKALRFIEEVTTNADAVQQRVLAEILSRNANTEYVNRFRLN 60

Query: 61  GATDRDTFKSKLPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPT 120
           GATDRDT+KSK+PVVTYEDLQPDIQRIANGDRSPIFSS PISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDRDTYKSKIPVVTYEDLQPDIQRIANGDRSPIFSSTPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD 180
           I+EEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD
Sbjct: 121 IQEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD 180

Query: 181 IFKTRPYDPFNDYTSPNEAILCADSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHF 240
           IFKTRPYDPFNDYTSPNEA+LCADSFQSMYTQMLCGLLMR QVLRVGAVFASGLLRAI F
Sbjct: 181 IFKTRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIRF 240

Query: 241 LQLNWKQLAHDISTGTLNPKITDPSLRECIISKYLTNPNPELAEFISKECSTEEWEGIVT 300
           LQLNWKQLAHDISTGTLNPKITDP LRECI+SKYLTNPNPE+AEFISKECSTEEWEGI+T
Sbjct: 241 LQLNWKQLAHDISTGTLNPKITDPCLRECIVSKYLTNPNPEVAEFISKECSTEEWEGIIT 300

Query: 301 RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTY 360
           RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTY
Sbjct: 301 RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTY 360

Query: 361 TIMPNMGYFEFLPHDTSSPPALSRDSPPRLVDLADVEVGKEYELVITTYSGLCRYRVGDI 420
           TIMPNMGYFEFLPH TSSPP LSRDSPPRLVDLADVEVGKEYELVITTY+GL RYRVGD+
Sbjct: 361 TIMPNMGYFEFLPHHTSSPPVLSRDSPPRLVDLADVEVGKEYELVITTYAGLRRYRVGDV 420

Query: 421 LQVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAIENASSLLREFNTSVVEYTSYA 480
           L+VTGFHNAAPQFRFVRRKNVLLSID+DKTDEAELQKA+ENASSLLRE NTSVVEYTSYA
Sbjct: 421 LEVTGFHNAAPQFRFVRRKNVLLSIDADKTDEAELQKAVENASSLLRELNTSVVEYTSYA 480

Query: 481 DTKTIPGHYVIYWELRVKD-AANS-PPEEILNQCCLAMEESLNSVYRQGRVADNSIGPLE 540
           DTKTIPGHYVIYWEL VKD AANS PP EILNQCCLA+EESLNSVYRQGRVADNSIGPLE
Sbjct: 481 DTKTIPGHYVIYWELLVKDEAANSPPPAEILNQCCLAIEESLNSVYRQGRVADNSIGPLE 540

Query: 541 IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPE 600
           IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRV SVHFSP+KPHWTPE
Sbjct: 541 IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVVSVHFSPTKPHWTPE 600

Query: 601 RRR 602
           RRR
Sbjct: 601 RRR 603

BLAST of Spg013562 vs. ExPASy Swiss-Prot
Match: O82333 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=3702 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 964.5 bits (2492), Expect = 5.5e-280
Identity = 469/602 (77.91%), Postives = 524/602 (87.04%), Query Frame = 0

Query: 1   MAVDSQLSSPLGPPACDKDAKALRFIHDTTTNADSVQQRVLAEILTRNAHTEYVTRFRLN 60
           MAVDS LSSPLGPPAC+KDAKALRFI + T NAD+VQ+ +LAEIL RNA TEY+ RF L 
Sbjct: 1   MAVDSNLSSPLGPPACEKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLC 60

Query: 61  GATDRDTFKSKLPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPT 120
           GATDRDTFK+K+PV+TYEDLQP+IQRIA+GDRSPI S+HPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDRDTFKTKIPVITYEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD 180
           IKEE++RRQLLYSLLMP+MNLYVPGLDKGKG+YFLFVK+ETKTPGGL ARPVLTSYYKS+
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSE 180

Query: 181 IFKTRPYDPFNDYTSPNEAILCADSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHF 240
            F++RPYDP+N YTSPNEAILC DSFQSMYTQMLCGLL R  VLRVGAVFASGLLRAI F
Sbjct: 181 HFRSRPYDPYNVYTSPNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRF 240

Query: 241 LQLNWKQLAHDISTGTLNPKITDPSLRECIISKYLTNPNPELAEFISKECSTEEWEGIVT 300
           LQL+W + AHDI  G L+ +ITDPS+R+C+    +  P+P LAEFI +EC ++ WE I+T
Sbjct: 241 LQLHWSRFAHDIELGCLDSEITDPSIRQCMSG--ILKPDPVLAEFIRRECKSDNWEKIIT 300

Query: 301 RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTY 360
           RIWPNTKYLDVIVTGAMAQYIPTLE+YSGGLPMACTMYASSECYFG+NL PM KPSEV+Y
Sbjct: 301 RIWPNTKYLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSY 360

Query: 361 TIMPNMGYFEFLPHDTSSPPALSRDSPPRLVDLADVEVGKEYELVITTYSGLCRYRVGDI 420
           TIMPNM YFEF+P   +           + V+L DV +GKEYELV+TTY+GLCRYRVGDI
Sbjct: 361 TIMPNMAYFEFIPLGGT-----------KAVELVDVNIGKEYELVVTTYAGLCRYRVGDI 420

Query: 421 LQVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAIENASSLL-REFNTSVVEYTSY 480
           L+VTGFHN+APQF FVRRKNVLLSIDSDKTDE+ELQKA+ENASS+L  E  + V EYTSY
Sbjct: 421 LRVTGFHNSAPQFHFVRRKNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSY 480

Query: 481 ADTKTIPGHYVIYWELRVKDAANSPPEEILNQCCLAMEESLNSVYRQGRVADNSIGPLEI 540
           ADT TIPGHYV+YWEL V+D A  P  E L +CCL MEESLNSVYRQ RVADNS+GPLEI
Sbjct: 481 ADTSTIPGHYVLYWELLVRDGARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEI 540

Query: 541 RVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPER 600
           RVV+NGTFEELMDYAISRGASINQYK PRCVNFTPIVELLDSRV S HFSPS PHWTPER
Sbjct: 541 RVVRNGTFEELMDYAISRGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPER 589

Query: 601 RR 602
           RR
Sbjct: 601 RR 589

BLAST of Spg013562 vs. ExPASy Swiss-Prot
Match: O22190 (Indole-3-acetic acid-amido synthetase GH3.3 OS=Arabidopsis thaliana OX=3702 GN=GH3.3 PE=1 SV=1)

HSP 1 Score: 918.3 bits (2372), Expect = 4.5e-266
Identity = 458/602 (76.08%), Postives = 515/602 (85.55%), Query Frame = 0

Query: 1   MAVDSQLSSPLGPPACDKDAKALRFIHDTTTNADSVQQRVLAEILTRNAHTEYVTRFRLN 60
           M VDS L SP+      KD KALRFI + T N D VQ++V+ EIL+RN+ TEY+ RF L 
Sbjct: 1   MTVDSALRSPMMHSPSTKDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLK 60

Query: 61  GATDRDTFKSKLPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPT 120
           G TDR TFK+K+PVV Y+DL+P+IQRIANGDRS I SS+PI+EFLTSSGTSAGERKLMPT
Sbjct: 61  GFTDRKTFKTKVPVVIYDDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPT 120

Query: 121 IKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD 180
           I E+M+RRQLLYSLLMP+MNLYVPGLDKGK LYFLFVK E+KTPGGL ARPVLTSYYKS+
Sbjct: 121 IDEDMDRRQLLYSLLMPVMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSE 180

Query: 181 IFKTRPYDPFNDYTSPNEAILCADSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHF 240
            FK RP DP+N YTSPNEAILC DS QSMYTQMLCGLLMR +VLR+GAVFASGLLRAI F
Sbjct: 181 QFKRRPNDPYNVYTSPNEAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGF 240

Query: 241 LQLNWKQLAHDISTGTLNPKITDPSLRECIISKYLTNPNPELAEFISKECSTE-EWEGIV 300
           LQ NWK+LA DISTGTL+ +I+DP+++E  +SK LT P+ ELA+FI+  C  +  WEGI+
Sbjct: 241 LQTNWKELADDISTGTLSSRISDPAIKES-MSKILTKPDQELADFITSVCGQDNSWEGII 300

Query: 301 TRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVT 360
           T+IWPNTKYLDVIVTGAMAQYIP LE+YSGGLPMACTMYASSE YFG+NLKPMCKPSEV+
Sbjct: 301 TKIWPNTKYLDVIVTGAMAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVS 360

Query: 361 YTIMPNMGYFEFLPHDTSSPPALSRDSPPRLVDLADVEVGKEYELVITTYSGLCRYRVGD 420
           YTIMPNM YFEFLPH    P   S      LV+LADVEVGKEYELVITTY+GL RYRVGD
Sbjct: 361 YTIMPNMAYFEFLPHH-EVPTEKS-----ELVELADVEVGKEYELVITTYAGLNRYRVGD 420

Query: 421 ILQVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAIENASSLLREFNTSVVEYTSY 480
           ILQVTGF+N+APQF+FVRRKNVLLSI+SDKTDEAELQ A+ENAS LL E  T V+EYTSY
Sbjct: 421 ILQVTGFYNSAPQFKFVRRKNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSY 480

Query: 481 ADTKTIPGHYVIYWELRVKDAANSPPEEILNQCCLAMEESLNSVYRQGRVADNSIGPLEI 540
           A+TKTIPGHYVIYWEL VKD  N P +E++ +CCL MEESLNSVYRQ RVAD SIGPLEI
Sbjct: 481 AETKTIPGHYVIYWELLVKDQTNPPNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLEI 540

Query: 541 RVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPER 600
           RVVKNGTFEELMDYAISRGASINQYK PRCV+FTPI+ELLDSRV S HFSP+ PHW+PER
Sbjct: 541 RVVKNGTFEELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSTHFSPALPHWSPER 595

Query: 601 RR 602
           RR
Sbjct: 601 RR 595

BLAST of Spg013562 vs. ExPASy Swiss-Prot
Match: A3BLS0 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=39946 GN=GH3.8 PE=1 SV=2)

HSP 1 Score: 894.4 bits (2310), Expect = 6.9e-259
Identity = 444/595 (74.62%), Postives = 503/595 (84.54%), Query Frame = 0

Query: 7   LSSPLGPPACDKDAKALRFIHDTTTNADSVQQRVLAEILTRNAHTEYVTRFRLNGATDRD 66
           L +P      + D + LRFI + TTN D+VQ+RVL EIL RNA TEY+T+  L+GATDR 
Sbjct: 14  LRTPAAGAVKEGDVEKLRFIDEMTTNVDAVQERVLGEILGRNAGTEYLTKCGLDGATDRA 73

Query: 67  TFKSKLPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEME 126
            F++K+PVV+Y+DLQP IQRIANGDRSPI S+HP+SEFLTSSGTSAGERKLMPTI +E++
Sbjct: 74  AFRAKVPVVSYDDLQPYIQRIANGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIMDELD 133

Query: 127 RRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFKTRP 186
           RRQLLYSLLMP+MNLYVPGLDKGKGLYFLFVK+ETKTPGGL ARPVLTSYYKSD FK RP
Sbjct: 134 RRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETKTPGGLTARPVLTSYYKSDHFKNRP 193

Query: 187 YDPFNDYTSPNEAILCADSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHFLQLNWK 246
           YDP+++YTSP  AILCAD+FQSMY QM+CGL  R  VLR+GAVFASGLLRAI FLQLNW+
Sbjct: 194 YDPYHNYTSPTAAILCADAFQSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLNWE 253

Query: 247 QLAHDISTGTLNPKITDPSLRECIISKYLTNPNPELAEFISKECSTEEWEGIVTRIWPNT 306
           QLA DI +G L P++TDPS+RE + +  L  P+PELA+ I  ECS  +W GI+TR+WPNT
Sbjct: 254 QLADDIESGELTPRVTDPSVREAVAAILL--PDPELAKLIRAECSKGDWAGIITRVWPNT 313

Query: 307 KYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTYTIMPNM 366
           KYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFG+NL+PMC PSEV+YTIMPNM
Sbjct: 314 KYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNM 373

Query: 367 GYFEFLPHDTSSPPALSRDSPPRLVDLADVEVGKEYELVITTYSGLCRYRVGDILQVTGF 426
           GYFEFLP D +   A S D+  +LVDLA VEVG+EYELVITTY+GL RYRVGD+L+VTGF
Sbjct: 374 GYFEFLPVDETG--AASGDA-TQLVDLARVEVGREYELVITTYAGLNRYRVGDVLRVTGF 433

Query: 427 HNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAIENASSLLREFNTSVVEYTSYADTKTIP 486
           HNAAPQFRFVRRKNVLLSI+SDKTDEAELQ+A+E AS+LLR    SVVEYTS A TK IP
Sbjct: 434 HNAAPQFRFVRRKNVLLSIESDKTDEAELQRAVERASALLRPHGASVVEYTSQACTKRIP 493

Query: 487 GHYVIYWELRVKDA-ANSPPEEILNQCCLAMEESLNSVYRQGRVADNSIGPLEIRVVKNG 546
           GHYVIYWEL  K A A     + L +CCL MEE+LN+VYRQ RVAD SIGPLEIRVV+ G
Sbjct: 494 GHYVIYWELLTKGAGATVVDADTLGRCCLEMEEALNTVYRQSRVADGSIGPLEIRVVRPG 553

Query: 547 TFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPERR 601
           TFEELMDYAISRGASINQYK PRCV F PIVELLDSRV S HFSP+ PHWTP RR
Sbjct: 554 TFEELMDYAISRGASINQYKVPRCVTFPPIVELLDSRVVSSHFSPALPHWTPARR 603

BLAST of Spg013562 vs. ExPASy Swiss-Prot
Match: Q0D4Z6 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.8 PE=1 SV=1)

HSP 1 Score: 894.4 bits (2310), Expect = 6.9e-259
Identity = 444/595 (74.62%), Postives = 503/595 (84.54%), Query Frame = 0

Query: 7   LSSPLGPPACDKDAKALRFIHDTTTNADSVQQRVLAEILTRNAHTEYVTRFRLNGATDRD 66
           L +P      + D + LRFI + TTN D+VQ+RVL EIL RNA TEY+T+  L+GATDR 
Sbjct: 14  LRTPAAGAVKEGDVEKLRFIDEMTTNVDAVQERVLGEILGRNAGTEYLTKCGLDGATDRA 73

Query: 67  TFKSKLPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEME 126
            F++K+PVV+Y+DLQP IQRIANGDRSPI S+HP+SEFLTSSGTSAGERKLMPTI +E++
Sbjct: 74  AFRAKVPVVSYDDLQPYIQRIANGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIMDELD 133

Query: 127 RRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFKTRP 186
           RRQLLYSLLMP+MNLYVPGLDKGKGLYFLFVK+ETKTPGGL ARPVLTSYYKSD FK RP
Sbjct: 134 RRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETKTPGGLTARPVLTSYYKSDHFKNRP 193

Query: 187 YDPFNDYTSPNEAILCADSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHFLQLNWK 246
           YDP+++YTSP  AILCAD+FQSMY QM+CGL  R  VLR+GAVFASGLLRAI FLQLNW+
Sbjct: 194 YDPYHNYTSPTAAILCADAFQSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLNWE 253

Query: 247 QLAHDISTGTLNPKITDPSLRECIISKYLTNPNPELAEFISKECSTEEWEGIVTRIWPNT 306
           QLA DI +G L P++TDPS+RE + +  L  P+PELA+ I  ECS  +W GI+TR+WPNT
Sbjct: 254 QLADDIESGELTPRVTDPSVREAVAAILL--PDPELAKLIRAECSKGDWAGIITRVWPNT 313

Query: 307 KYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTYTIMPNM 366
           KYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFG+NL+PMC PSEV+YTIMPNM
Sbjct: 314 KYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNM 373

Query: 367 GYFEFLPHDTSSPPALSRDSPPRLVDLADVEVGKEYELVITTYSGLCRYRVGDILQVTGF 426
           GYFEFLP D +   A S D+  +LVDLA VEVG+EYELVITTY+GL RYRVGD+L+VTGF
Sbjct: 374 GYFEFLPVDETG--AASGDA-TQLVDLARVEVGREYELVITTYAGLNRYRVGDVLRVTGF 433

Query: 427 HNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAIENASSLLREFNTSVVEYTSYADTKTIP 486
           HNAAPQFRFVRRKNVLLSI+SDKTDEAELQ+A+E AS+LLR    SVVEYTS A TK IP
Sbjct: 434 HNAAPQFRFVRRKNVLLSIESDKTDEAELQRAVERASALLRPHGASVVEYTSQACTKRIP 493

Query: 487 GHYVIYWELRVKDA-ANSPPEEILNQCCLAMEESLNSVYRQGRVADNSIGPLEIRVVKNG 546
           GHYVIYWEL  K A A     + L +CCL MEE+LN+VYRQ RVAD SIGPLEIRVV+ G
Sbjct: 494 GHYVIYWELLTKGAGATVVDADTLGRCCLEMEEALNTVYRQSRVADGSIGPLEIRVVRPG 553

Query: 547 TFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPERR 601
           TFEELMDYAISRGASINQYK PRCV F PIVELLDSRV S HFSP+ PHWTP RR
Sbjct: 554 TFEELMDYAISRGASINQYKVPRCVTFPPIVELLDSRVVSSHFSPALPHWTPARR 603

BLAST of Spg013562 vs. ExPASy Swiss-Prot
Match: P0C0M2 (Probable indole-3-acetic acid-amido synthetase GH3.2 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.2 PE=2 SV=1)

HSP 1 Score: 887.5 bits (2292), Expect = 8.5e-257
Identity = 430/593 (72.51%), Postives = 508/593 (85.67%), Query Frame = 0

Query: 15  ACDKDAKALRFIHDTTTNADSVQQRVLAEILTRNAHTEYVTRFRLNGATDRDTFKSKLPV 74
           AC++DA+ L FI + T   D+VQ+RVLA IL RN   EY+ R  + G TDR+ FK+++PV
Sbjct: 25  ACERDAEKLEFIEEMTRGFDAVQERVLAAILARNNGAEYLRRHGMEGRTDREAFKARVPV 84

Query: 75  VTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQLLYSL 134
           VTYEDL+P+I+RIANGDRS I SSHPI+EFLTSSGTSAGERKLMPTI++E++RRQ+LYSL
Sbjct: 85  VTYEDLRPEIERIANGDRSNIISSHPITEFLTSSGTSAGERKLMPTIEDELDRRQMLYSL 144

Query: 135 LMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFKTRPYDPFNDYT 194
           LMP+MNLYVPGLDKGKGLYFLF+K+ETKTPGGL ARPVLTSYYKSD FK RP+DP+N YT
Sbjct: 145 LMPVMNLYVPGLDKGKGLYFLFIKSETKTPGGLPARPVLTSYYKSDHFKHRPFDPYNVYT 204

Query: 195 SPNEAILCADSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHFLQLNWKQLAHDIST 254
           SP  AILC D+FQSMY QMLCGL+ R +VLRVGAVFASGLLRAI FLQL+W++LAHDI T
Sbjct: 205 SPTAAILCTDAFQSMYAQMLCGLVARAEVLRVGAVFASGLLRAIRFLQLHWRELAHDIRT 264

Query: 255 GTLNPKITDPSLRECIISKYLTNPNPELAEFISKECSTEEWEGIVTRIWPNTKYLDVIVT 314
           GTL+ K+T+PS+R+  +++ L  P+ ELA F+  EC  ++WEGI+TR+WPNTKYLDVIVT
Sbjct: 265 GTLSAKVTEPSIRDA-VAEVLAAPDAELAAFVEAECGKDKWEGIITRMWPNTKYLDVIVT 324

Query: 315 GAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTYTIMPNMGYFEFLPH 374
           GAMAQYIPTL+FYSGGLPMACTMYASSECYFG+NL+PMC PSEV+YTIMPNMGYFE +PH
Sbjct: 325 GAMAQYIPTLKFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFELMPH 384

Query: 375 DTSSPPALSRDS-PPRLVDLADVEVGKEYELVITTYSGLCRYRVGDILQVTGFHNAAPQF 434
           D  +PP L RD+ PPRLVDLAD EVG+EYELVITTY+GLCRYRVGDILQVTGFHNAAPQF
Sbjct: 385 DPDAPP-LPRDAPPPRLVDLADAEVGREYELVITTYAGLCRYRVGDILQVTGFHNAAPQF 444

Query: 435 RFVRRKNVLLSIDSDKTDEAELQKAIENASSLLREFNTSVVEYTSYADTKTIPGHYVIYW 494
           RFVRRKNVLLSIDSDKTDEAELQ A+E AS+LL  +  S+VEYTS AD  TIPGHYV+YW
Sbjct: 445 RFVRRKNVLLSIDSDKTDEAELQAAVERASALLSPYGASIVEYTSQADATTIPGHYVVYW 504

Query: 495 ELRVKDAANSPP---EE---ILNQCCLAMEESLNSVYRQGRVADNSIGPLEIRVVKNGTF 554
           EL V++    PP   EE   +  +CCL MEE+LN+VYRQGR  + +IGPLEIRVV+ GTF
Sbjct: 505 ELMVREGGAWPPPAEEEGRGVFERCCLEMEEALNAVYRQGRNGE-AIGPLEIRVVRAGTF 564

Query: 555 EELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPERR 601
           EE+MDYAISRGASINQYKAPRCV+F PI+ELL+SRV S HFSP+ P ++P ++
Sbjct: 565 EEVMDYAISRGASINQYKAPRCVSFGPIIELLNSRVISKHFSPACPKYSPHKK 614

BLAST of Spg013562 vs. ExPASy TrEMBL
Match: A0A6J1GH54 (probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita moschata OX=3662 GN=LOC111453877 PE=3 SV=1)

HSP 1 Score: 1146.3 bits (2964), Expect = 0.0e+00
Identity = 566/603 (93.86%), Postives = 583/603 (96.68%), Query Frame = 0

Query: 1   MAVDSQLSSPLGPPACDKDAKALRFIHDTTTNADSVQQRVLAEILTRNAHTEYVTRFRLN 60
           MAVDSQLSSPL PP  DKDAKALRFI + TTN D+VQQRVLAEIL+RNA+TEYV RFRLN
Sbjct: 1   MAVDSQLSSPLPPPPSDKDAKALRFIEEVTTNTDAVQQRVLAEILSRNANTEYVNRFRLN 60

Query: 61  GATDRDTFKSKLPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPT 120
           GATDRDT+KSK+PVVTYEDLQPDIQRIANGDRSPIFSS PISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDRDTYKSKIPVVTYEDLQPDIQRIANGDRSPIFSSTPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD 180
           I+EEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD
Sbjct: 121 IQEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD 180

Query: 181 IFKTRPYDPFNDYTSPNEAILCADSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHF 240
           IFKTRPYDPFNDYTSPNEA+LCADSFQSMYTQMLCGLLMR QVLRVGAVFASGLLRAI F
Sbjct: 181 IFKTRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIRF 240

Query: 241 LQLNWKQLAHDISTGTLNPKITDPSLRECIISKYLTNPNPELAEFISKECSTEEWEGIVT 300
           LQLNWKQLAHDISTGTLNPKITDP LRECI+SKYLTN NPE+AEFISKECSTEEWEGI+T
Sbjct: 241 LQLNWKQLAHDISTGTLNPKITDPCLRECIVSKYLTNTNPEVAEFISKECSTEEWEGIIT 300

Query: 301 RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTY 360
           RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTY
Sbjct: 301 RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTY 360

Query: 361 TIMPNMGYFEFLPHDTSSPPALSRDSPPRLVDLADVEVGKEYELVITTYSGLCRYRVGDI 420
           TIMPNMGYFEFLPHDTSSPP LSRDSPPRLVDLADVEVGKEYELVITTY+GL RYRVGD+
Sbjct: 361 TIMPNMGYFEFLPHDTSSPPVLSRDSPPRLVDLADVEVGKEYELVITTYAGLRRYRVGDV 420

Query: 421 LQVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAIENASSLLREFNTSVVEYTSYA 480
           L+VTGFHNAAPQFRFVRRKNVLLSID+DKTDEAELQKA+ENASSLLRE NTSVVEYTSYA
Sbjct: 421 LEVTGFHNAAPQFRFVRRKNVLLSIDADKTDEAELQKAVENASSLLRELNTSVVEYTSYA 480

Query: 481 DTKTIPGHYVIYWELRVKD-AANS-PPEEILNQCCLAMEESLNSVYRQGRVADNSIGPLE 540
           DTKTIPGHYVIYWEL VKD AANS PP EILNQCCLA+EESLNSVYRQGRVADNSIGPLE
Sbjct: 481 DTKTIPGHYVIYWELLVKDEAANSPPPAEILNQCCLAIEESLNSVYRQGRVADNSIGPLE 540

Query: 541 IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPE 600
           IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRV SVHFSPSKPHWTPE
Sbjct: 541 IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVASVHFSPSKPHWTPE 600

Query: 601 RRR 602
           RRR
Sbjct: 601 RRR 603

BLAST of Spg013562 vs. ExPASy TrEMBL
Match: A0A6J1IRM5 (probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita maxima OX=3661 GN=LOC111478730 PE=3 SV=1)

HSP 1 Score: 1143.3 bits (2956), Expect = 0.0e+00
Identity = 564/603 (93.53%), Postives = 583/603 (96.68%), Query Frame = 0

Query: 1   MAVDSQLSSPLGPPACDKDAKALRFIHDTTTNADSVQQRVLAEILTRNAHTEYVTRFRLN 60
           MAV SQLSSPL PP  D+DAKALRFI + TTNAD+VQQRVLAEIL+RNA+TEYV RFRLN
Sbjct: 1   MAVGSQLSSPLPPPPSDRDAKALRFIEEVTTNADAVQQRVLAEILSRNANTEYVNRFRLN 60

Query: 61  GATDRDTFKSKLPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPT 120
           GATDRDT+KSK+PVVTYEDLQPDIQRIANGDRSPIFSS PISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDRDTYKSKIPVVTYEDLQPDIQRIANGDRSPIFSSTPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD 180
           I+EEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD
Sbjct: 121 IQEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD 180

Query: 181 IFKTRPYDPFNDYTSPNEAILCADSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHF 240
           IFKTRPYDPFNDYTSPNEA+LCADSFQSMYTQMLCGLLMR QVLRVGAVFASGLLRAI F
Sbjct: 181 IFKTRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAIRF 240

Query: 241 LQLNWKQLAHDISTGTLNPKITDPSLRECIISKYLTNPNPELAEFISKECSTEEWEGIVT 300
           LQLNWKQLAHDISTGTLNPKITDP LRECI+SKYLTNPNPE+AEFISKECSTEEWEGI+T
Sbjct: 241 LQLNWKQLAHDISTGTLNPKITDPCLRECIVSKYLTNPNPEVAEFISKECSTEEWEGIIT 300

Query: 301 RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTY 360
           RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTY
Sbjct: 301 RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTY 360

Query: 361 TIMPNMGYFEFLPHDTSSPPALSRDSPPRLVDLADVEVGKEYELVITTYSGLCRYRVGDI 420
           TIMPNMGYFEFLPH TSSPP LSRDSPPRLVDLADVEVGKEYELVITTY+GL RYRVGD+
Sbjct: 361 TIMPNMGYFEFLPHHTSSPPVLSRDSPPRLVDLADVEVGKEYELVITTYAGLRRYRVGDV 420

Query: 421 LQVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAIENASSLLREFNTSVVEYTSYA 480
           L+VTGFHNAAPQFRFVRRKNVLLSID+DKTDEAELQKA+ENASSLLRE NTSVVEYTSYA
Sbjct: 421 LEVTGFHNAAPQFRFVRRKNVLLSIDADKTDEAELQKAVENASSLLRELNTSVVEYTSYA 480

Query: 481 DTKTIPGHYVIYWELRVKD-AANS-PPEEILNQCCLAMEESLNSVYRQGRVADNSIGPLE 540
           DTKTIPGHYVIYWEL VKD AANS PP EILNQCCLA+EESLNSVYRQGRVADNSIGPLE
Sbjct: 481 DTKTIPGHYVIYWELLVKDEAANSPPPAEILNQCCLAIEESLNSVYRQGRVADNSIGPLE 540

Query: 541 IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPE 600
           IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRV SVHFSP+KPHWTPE
Sbjct: 541 IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVVSVHFSPTKPHWTPE 600

Query: 601 RRR 602
           RRR
Sbjct: 601 RRR 603

BLAST of Spg013562 vs. ExPASy TrEMBL
Match: A0A5D3CNV8 (Putative indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G004400 PE=3 SV=1)

HSP 1 Score: 1137.5 bits (2941), Expect = 0.0e+00
Identity = 561/602 (93.19%), Postives = 579/602 (96.18%), Query Frame = 0

Query: 4   DSQLSSPLGPPACDKDAKALRFIHDTTTNADSVQQRVLAEILTRNAHTEYVTRFRLNGAT 63
           +S+ SSPLGPPAC+KDAKALRFI +TTTNAD VQQRVLAEILT+NAHTEYV RFRLNGAT
Sbjct: 6   NSEFSSPLGPPACEKDAKALRFIEETTTNADLVQQRVLAEILTQNAHTEYVNRFRLNGAT 65

Query: 64  DRDTFKSKLPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKE 123
           DRDTFKSK PVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKE
Sbjct: 66  DRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKE 125

Query: 124 EMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFK 183
           EMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFK
Sbjct: 126 EMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFK 185

Query: 184 TRPYDPFNDYTSPNEAILCADSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHFLQL 243
           TRPYDPFNDYTSPNEA+LCADSFQSMYTQMLCGLLMR QVLRVGAVFASGLLRAI FLQL
Sbjct: 186 TRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIRFLQL 245

Query: 244 NWKQLAHDISTGTLNPKITDPSLRECIISKYLTNPNPELAEFISKECSTEEWEGIVTRIW 303
           NWKQLAHDISTGTLNPKITD  LRECI SKYLTNPNPELAEFIS EC TEEWEGI+TRIW
Sbjct: 246 NWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPNPELAEFISNECYTEEWEGIITRIW 305

Query: 304 PNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTYTIM 363
           PNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNL PMCKPS+VTYTIM
Sbjct: 306 PNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIM 365

Query: 364 PNMGYFEFLPHDTSS-PPALSRDSPPRLVDLADVEVGKEYELVITTYSGLCRYRVGDILQ 423
           PNMGYFEFLPHD+SS   ALSRDSPPRLVDLADVEVGKEYELVITTY+GLCRYRVGD+L+
Sbjct: 366 PNMGYFEFLPHDSSSRASALSRDSPPRLVDLADVEVGKEYELVITTYAGLCRYRVGDVLE 425

Query: 424 VTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAIENASSLLREFNTSVVEYTSYADT 483
           VTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKA+ENASSLLR+FNT+VVEYTSYADT
Sbjct: 426 VTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLRDFNTTVVEYTSYADT 485

Query: 484 KTIPGHYVIYWELRVKD--AANS-PPEEILNQCCLAMEESLNSVYRQGRVADNSIGPLEI 543
           KTIPGHYVIYWEL VKD    NS P +EILNQCCLA+EESLNSVYRQGRVADNSIGPLEI
Sbjct: 486 KTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSIGPLEI 545

Query: 544 RVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPER 602
           RVV+NGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPER
Sbjct: 546 RVVRNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPER 605

BLAST of Spg013562 vs. ExPASy TrEMBL
Match: A0A1S3B0G2 (probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo OX=3656 GN=LOC103484816 PE=3 SV=1)

HSP 1 Score: 1137.5 bits (2941), Expect = 0.0e+00
Identity = 561/602 (93.19%), Postives = 579/602 (96.18%), Query Frame = 0

Query: 4   DSQLSSPLGPPACDKDAKALRFIHDTTTNADSVQQRVLAEILTRNAHTEYVTRFRLNGAT 63
           +S+ SSPLGPPAC+KDAKALRFI +TTTNAD VQQRVLAEILT+NAHTEYV RFRLNGAT
Sbjct: 6   NSEFSSPLGPPACEKDAKALRFIEETTTNADLVQQRVLAEILTQNAHTEYVNRFRLNGAT 65

Query: 64  DRDTFKSKLPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKE 123
           DRDTFKSK PVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKE
Sbjct: 66  DRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKE 125

Query: 124 EMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFK 183
           EMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFK
Sbjct: 126 EMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFK 185

Query: 184 TRPYDPFNDYTSPNEAILCADSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHFLQL 243
           TRPYDPFNDYTSPNEA+LCADSFQSMYTQMLCGLLMR QVLRVGAVFASGLLRAI FLQL
Sbjct: 186 TRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIRFLQL 245

Query: 244 NWKQLAHDISTGTLNPKITDPSLRECIISKYLTNPNPELAEFISKECSTEEWEGIVTRIW 303
           NWKQLAHDISTGTLNPKITD  LRECI SKYLTNPNPELAEFIS EC TEEWEGI+TRIW
Sbjct: 246 NWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPNPELAEFISNECYTEEWEGIITRIW 305

Query: 304 PNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTYTIM 363
           PNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNL PMCKPS+VTYTIM
Sbjct: 306 PNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIM 365

Query: 364 PNMGYFEFLPHDTSS-PPALSRDSPPRLVDLADVEVGKEYELVITTYSGLCRYRVGDILQ 423
           PNMGYFEFLPHD+SS   ALSRDSPPRLVDLADVEVGKEYELVITTY+GLCRYRVGD+L+
Sbjct: 366 PNMGYFEFLPHDSSSRASALSRDSPPRLVDLADVEVGKEYELVITTYAGLCRYRVGDVLE 425

Query: 424 VTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAIENASSLLREFNTSVVEYTSYADT 483
           VTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKA+ENASSLLR+FNT+VVEYTSYADT
Sbjct: 426 VTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLRDFNTTVVEYTSYADT 485

Query: 484 KTIPGHYVIYWELRVKD--AANS-PPEEILNQCCLAMEESLNSVYRQGRVADNSIGPLEI 543
           KTIPGHYVIYWEL VKD    NS P +EILNQCCLA+EESLNSVYRQGRVADNSIGPLEI
Sbjct: 486 KTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSIGPLEI 545

Query: 544 RVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPER 602
           RVV+NGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPER
Sbjct: 546 RVVRNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPER 605

BLAST of Spg013562 vs. ExPASy TrEMBL
Match: A0A0A0KJM5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G493310 PE=3 SV=1)

HSP 1 Score: 1131.3 bits (2925), Expect = 0.0e+00
Identity = 559/602 (92.86%), Postives = 576/602 (95.68%), Query Frame = 0

Query: 4   DSQLSSPLGPPACDKDAKALRFIHDTTTNADSVQQRVLAEILTRNAHTEYVTRFRLNGAT 63
           +S+ S+PLGPPAC+KDAKALRFI +TTTN DSVQQRVLAEILT+NAHTEYV RFRLNGAT
Sbjct: 6   NSEFSTPLGPPACEKDAKALRFIEETTTNTDSVQQRVLAEILTQNAHTEYVNRFRLNGAT 65

Query: 64  DRDTFKSKLPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKE 123
           DRDTFKSK PVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKE
Sbjct: 66  DRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKE 125

Query: 124 EMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFK 183
           EMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFK
Sbjct: 126 EMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFK 185

Query: 184 TRPYDPFNDYTSPNEAILCADSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHFLQL 243
           TR YDPFNDYTSPNEA+LCADSFQSMYTQMLCGLLMR QVLRVGAVFASGLLRAI FLQL
Sbjct: 186 TRHYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIRFLQL 245

Query: 244 NWKQLAHDISTGTLNPKITDPSLRECIISKYLTNPNPELAEFISKECSTEEWEGIVTRIW 303
           NWKQLAHDISTGTLNPKITD  LRECI SKYLTNP PELAEFIS EC TEEWEGI+TRIW
Sbjct: 246 NWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPKPELAEFISSECCTEEWEGIITRIW 305

Query: 304 PNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTYTIM 363
           PNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNL PMCKPS+VTYTIM
Sbjct: 306 PNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIM 365

Query: 364 PNMGYFEFLPHDTSS-PPALSRDSPPRLVDLADVEVGKEYELVITTYSGLCRYRVGDILQ 423
           PNMGYFEFLPHD+SS  PALSRDSPPRLVDLADVEVGKEYELVITTY+GL RYRVGD+L+
Sbjct: 366 PNMGYFEFLPHDSSSRAPALSRDSPPRLVDLADVEVGKEYELVITTYAGLVRYRVGDVLE 425

Query: 424 VTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAIENASSLLREFNTSVVEYTSYADT 483
           VTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKA+ENASSLLREFNT+VVEYTSYADT
Sbjct: 426 VTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYTSYADT 485

Query: 484 KTIPGHYVIYWELRVKD--AANS-PPEEILNQCCLAMEESLNSVYRQGRVADNSIGPLEI 543
           KTIPGHYVIYWEL VKD    NS P +EILNQCCLA+EESLNSVYRQGRVADNSIGPLEI
Sbjct: 486 KTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSIGPLEI 545

Query: 544 RVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPER 602
           RVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTS HFSPSKPHWTPER
Sbjct: 546 RVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSFHFSPSKPHWTPER 605

BLAST of Spg013562 vs. TAIR 10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 964.5 bits (2492), Expect = 3.9e-281
Identity = 469/602 (77.91%), Postives = 524/602 (87.04%), Query Frame = 0

Query: 1   MAVDSQLSSPLGPPACDKDAKALRFIHDTTTNADSVQQRVLAEILTRNAHTEYVTRFRLN 60
           MAVDS LSSPLGPPAC+KDAKALRFI + T NAD+VQ+ +LAEIL RNA TEY+ RF L 
Sbjct: 1   MAVDSNLSSPLGPPACEKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLC 60

Query: 61  GATDRDTFKSKLPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPT 120
           GATDRDTFK+K+PV+TYEDLQP+IQRIA+GDRSPI S+HPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDRDTFKTKIPVITYEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD 180
           IKEE++RRQLLYSLLMP+MNLYVPGLDKGKG+YFLFVK+ETKTPGGL ARPVLTSYYKS+
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSE 180

Query: 181 IFKTRPYDPFNDYTSPNEAILCADSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHF 240
            F++RPYDP+N YTSPNEAILC DSFQSMYTQMLCGLL R  VLRVGAVFASGLLRAI F
Sbjct: 181 HFRSRPYDPYNVYTSPNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRF 240

Query: 241 LQLNWKQLAHDISTGTLNPKITDPSLRECIISKYLTNPNPELAEFISKECSTEEWEGIVT 300
           LQL+W + AHDI  G L+ +ITDPS+R+C+    +  P+P LAEFI +EC ++ WE I+T
Sbjct: 241 LQLHWSRFAHDIELGCLDSEITDPSIRQCMSG--ILKPDPVLAEFIRRECKSDNWEKIIT 300

Query: 301 RIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTY 360
           RIWPNTKYLDVIVTGAMAQYIPTLE+YSGGLPMACTMYASSECYFG+NL PM KPSEV+Y
Sbjct: 301 RIWPNTKYLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSY 360

Query: 361 TIMPNMGYFEFLPHDTSSPPALSRDSPPRLVDLADVEVGKEYELVITTYSGLCRYRVGDI 420
           TIMPNM YFEF+P   +           + V+L DV +GKEYELV+TTY+GLCRYRVGDI
Sbjct: 361 TIMPNMAYFEFIPLGGT-----------KAVELVDVNIGKEYELVVTTYAGLCRYRVGDI 420

Query: 421 LQVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAIENASSLL-REFNTSVVEYTSY 480
           L+VTGFHN+APQF FVRRKNVLLSIDSDKTDE+ELQKA+ENASS+L  E  + V EYTSY
Sbjct: 421 LRVTGFHNSAPQFHFVRRKNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSY 480

Query: 481 ADTKTIPGHYVIYWELRVKDAANSPPEEILNQCCLAMEESLNSVYRQGRVADNSIGPLEI 540
           ADT TIPGHYV+YWEL V+D A  P  E L +CCL MEESLNSVYRQ RVADNS+GPLEI
Sbjct: 481 ADTSTIPGHYVLYWELLVRDGARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEI 540

Query: 541 RVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPER 600
           RVV+NGTFEELMDYAISRGASINQYK PRCVNFTPIVELLDSRV S HFSPS PHWTPER
Sbjct: 541 RVVRNGTFEELMDYAISRGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPER 589

Query: 601 RR 602
           RR
Sbjct: 601 RR 589

BLAST of Spg013562 vs. TAIR 10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 925.6 bits (2391), Expect = 2.0e-269
Identity = 458/604 (75.83%), Postives = 521/604 (86.26%), Query Frame = 0

Query: 1   MAVDSQLSSPL-GPPACDKDAKALRFIHDTTTNADSVQQRVLAEILTRNAHTEYVTRFRL 60
           MAVDS L S +      +KD KAL+FI + T N DSVQ++VL EILTRN++TEY+ RF L
Sbjct: 1   MAVDSPLQSRMVSATTSEKDVKALKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRFDL 60

Query: 61  NGATDRDTFKSKLPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMP 120
           +G  DR TFKSK+PVVTYEDL+P+IQRI+NGD SPI SSHPI+EFLTSSGTSAGERKLMP
Sbjct: 61  DGVVDRKTFKSKVPVVTYEDLKPEIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMP 120

Query: 121 TIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKS 180
           TI+E+++RRQLLYSLLMP+MNLYVPGLDKGKGLYFLFVK+E+KT GGL ARPVLTSYYKS
Sbjct: 121 TIEEDLDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSESKTSGGLPARPVLTSYYKS 180

Query: 181 DIFKTRPYDPFNDYTSPNEAILCADSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIH 240
           D FK RPYDP+N YTSPNEAILC+DS QSMY QMLCGLLMR +VLR+GAVFASGLLRAI 
Sbjct: 181 DHFKRRPYDPYNVYTSPNEAILCSDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAIS 240

Query: 241 FLQLNWKQLAHDISTGTLNPKITDPSLRECIISKYLTNPNPELAEFISKECSTEEWEGIV 300
           FLQ NWK+LA DISTGTL+ +I DP+++   +SK LT P+ ELAEF+   CS E WEGI+
Sbjct: 241 FLQNNWKELARDISTGTLSSRIFDPAIKN-RMSKILTKPDQELAEFLVGVCSQENWEGII 300

Query: 301 TRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVT 360
           T+IWPNTKYLDVIVTGAMAQYIPTLE+YSGGLPMACTMYASSE YFG+NLKPMCKPSEV+
Sbjct: 301 TKIWPNTKYLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVS 360

Query: 361 YTIMPNMGYFEFLPHDTSSPPALSRD-SPPRLVDLADVEVGKEYELVITTYSGLCRYRVG 420
           YTIMPNM YFEFLPH+     A         LV+LA+VEVGKEYELVITTY+GL RYRVG
Sbjct: 361 YTIMPNMAYFEFLPHNHDGDGAAEASLDETSLVELANVEVGKEYELVITTYAGLYRYRVG 420

Query: 421 DILQVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAIENASSLLREFNTSVVEYTS 480
           DIL+VTGFHN+APQF+F+RRKNVLLS++SDKTDEAELQKA+ENAS L  E  T V+EYTS
Sbjct: 421 DILRVTGFHNSAPQFKFIRRKNVLLSVESDKTDEAELQKAVENASRLFAEQGTRVIEYTS 480

Query: 481 YADTKTIPGHYVIYWELRVKDAANS-PPEEILNQCCLAMEESLNSVYRQGRVADNSIGPL 540
           YA+TKTIPGHYVIYWEL  +D +N+   EE++ +CCL MEESLNSVYRQ RVAD SIGPL
Sbjct: 481 YAETKTIPGHYVIYWELLGRDQSNALMSEEVMAKCCLEMEESLNSVYRQSRVADKSIGPL 540

Query: 541 EIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTP 600
           EIRVV+NGTFEELMDYAISRGASINQYK PRCV+FTPI+ELLDSRV S HFSPS PHW+P
Sbjct: 541 EIRVVRNGTFEELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSAHFSPSLPHWSP 600

Query: 601 ERRR 602
           ERRR
Sbjct: 601 ERRR 603

BLAST of Spg013562 vs. TAIR 10
Match: AT2G23170.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 918.3 bits (2372), Expect = 3.2e-267
Identity = 458/602 (76.08%), Postives = 515/602 (85.55%), Query Frame = 0

Query: 1   MAVDSQLSSPLGPPACDKDAKALRFIHDTTTNADSVQQRVLAEILTRNAHTEYVTRFRLN 60
           M VDS L SP+      KD KALRFI + T N D VQ++V+ EIL+RN+ TEY+ RF L 
Sbjct: 1   MTVDSALRSPMMHSPSTKDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLK 60

Query: 61  GATDRDTFKSKLPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPT 120
           G TDR TFK+K+PVV Y+DL+P+IQRIANGDRS I SS+PI+EFLTSSGTSAGERKLMPT
Sbjct: 61  GFTDRKTFKTKVPVVIYDDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPT 120

Query: 121 IKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSD 180
           I E+M+RRQLLYSLLMP+MNLYVPGLDKGK LYFLFVK E+KTPGGL ARPVLTSYYKS+
Sbjct: 121 IDEDMDRRQLLYSLLMPVMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSE 180

Query: 181 IFKTRPYDPFNDYTSPNEAILCADSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHF 240
            FK RP DP+N YTSPNEAILC DS QSMYTQMLCGLLMR +VLR+GAVFASGLLRAI F
Sbjct: 181 QFKRRPNDPYNVYTSPNEAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGF 240

Query: 241 LQLNWKQLAHDISTGTLNPKITDPSLRECIISKYLTNPNPELAEFISKECSTE-EWEGIV 300
           LQ NWK+LA DISTGTL+ +I+DP+++E  +SK LT P+ ELA+FI+  C  +  WEGI+
Sbjct: 241 LQTNWKELADDISTGTLSSRISDPAIKES-MSKILTKPDQELADFITSVCGQDNSWEGII 300

Query: 301 TRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVT 360
           T+IWPNTKYLDVIVTGAMAQYIP LE+YSGGLPMACTMYASSE YFG+NLKPMCKPSEV+
Sbjct: 301 TKIWPNTKYLDVIVTGAMAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVS 360

Query: 361 YTIMPNMGYFEFLPHDTSSPPALSRDSPPRLVDLADVEVGKEYELVITTYSGLCRYRVGD 420
           YTIMPNM YFEFLPH    P   S      LV+LADVEVGKEYELVITTY+GL RYRVGD
Sbjct: 361 YTIMPNMAYFEFLPHH-EVPTEKS-----ELVELADVEVGKEYELVITTYAGLNRYRVGD 420

Query: 421 ILQVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAIENASSLLREFNTSVVEYTSY 480
           ILQVTGF+N+APQF+FVRRKNVLLSI+SDKTDEAELQ A+ENAS LL E  T V+EYTSY
Sbjct: 421 ILQVTGFYNSAPQFKFVRRKNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSY 480

Query: 481 ADTKTIPGHYVIYWELRVKDAANSPPEEILNQCCLAMEESLNSVYRQGRVADNSIGPLEI 540
           A+TKTIPGHYVIYWEL VKD  N P +E++ +CCL MEESLNSVYRQ RVAD SIGPLEI
Sbjct: 481 AETKTIPGHYVIYWELLVKDQTNPPNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLEI 540

Query: 541 RVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPER 600
           RVVKNGTFEELMDYAISRGASINQYK PRCV+FTPI+ELLDSRV S HFSP+ PHW+PER
Sbjct: 541 RVVKNGTFEELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSTHFSPALPHWSPER 595

Query: 601 RR 602
           RR
Sbjct: 601 RR 595

BLAST of Spg013562 vs. TAIR 10
Match: AT1G59500.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 862.4 bits (2227), Expect = 2.1e-250
Identity = 430/602 (71.43%), Postives = 503/602 (83.55%), Query Frame = 0

Query: 1   MAVDSQLSSPL-GPPACDKDAKALRFIHDTTTNADSVQQRVLAEILTRNAHTEYVTRFRL 60
           MAVDS L S +  P   + + KAL+FI + T N DSVQ++VL EIL+RN++TEY+ RF L
Sbjct: 1   MAVDSLLQSGMASPTTSETEVKALKFIEEITRNPDSVQEKVLGEILSRNSNTEYLKRFDL 60

Query: 61  NGATDRDTFKSKLPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMP 120
           NGA DR +FKSK+PVV YEDL+ DIQRI+NGDRSPI SSHPI+EFLTSSGTSAGERKLMP
Sbjct: 61  NGAVDRKSFKSKVPVVIYEDLKTDIQRISNGDRSPILSSHPITEFLTSSGTSAGERKLMP 120

Query: 121 TIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKS 180
           TI+E++ RRQLL +LLMP+MNLYVPGLDKGKGLYFLFVK+E+ T GGL ARP LTSYYKS
Sbjct: 121 TIEEDINRRQLLGNLLMPVMNLYVPGLDKGKGLYFLFVKSESTTSGGLPARPALTSYYKS 180

Query: 181 DIFKTRPYDPFNDYTSPNEAILCADSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIH 240
           D F+T   D  + YTSP EAILC DS QSMYTQMLCGLLMR +V R+GAVF SGLLRAI 
Sbjct: 181 DYFRTSDSD--SVYTSPKEAILCCDSSQSMYTQMLCGLLMRHEVNRLGAVFPSGLLRAIS 240

Query: 241 FLQLNWKQLAHDISTGTLNPKITDPSLRECIISKYLTNPNPELAEFISKECSTEEWEGIV 300
           FLQ NWK+L+ DISTGTL+ KI D +++   +S  L  P+ ELAEF+   CS E WEGI+
Sbjct: 241 FLQNNWKELSQDISTGTLSSKIFDHAIK-TRMSNILNKPDQELAEFLIGVCSQENWEGII 300

Query: 301 TRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVT 360
           T+IWPNTKYLDVIVTGAMA+YIP LE+YSGGLPMA  +YASSE YFG+NL PMCKPSEV+
Sbjct: 301 TKIWPNTKYLDVIVTGAMAEYIPMLEYYSGGLPMASMIYASSESYFGINLNPMCKPSEVS 360

Query: 361 YTIMPNMGYFEFLPHDTSSPPALSRDSPPRLVDLADVEVGKEYELVITTYSGLCRYRVGD 420
           YTI PNM YFEFLPH+      +   S   LV+LADVEVGKEYELVITTY+GL RYRVGD
Sbjct: 361 YTIFPNMAYFEFLPHNHDGDGGVEATS---LVELADVEVGKEYELVITTYAGLYRYRVGD 420

Query: 421 ILQVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAIENASSLLREFNTSVVEYTSY 480
           IL+VTGFHN+APQF+F+RR+NVLLSI+SDKTDEA+LQKA+ENAS LL E  T V+EYTSY
Sbjct: 421 ILRVTGFHNSAPQFKFIRRENVLLSIESDKTDEADLQKAVENASRLLAEQGTRVIEYTSY 480

Query: 481 ADTKTIPGHYVIYWELRVKDAANS-PPEEILNQCCLAMEESLNSVYRQGRVADNSIGPLE 540
           ADTKTIPGHYVIYWEL  +D +N+ P +E++ +CCL MEESLN+VYRQ RV+D SIGPLE
Sbjct: 481 ADTKTIPGHYVIYWELLSRDQSNALPSDEVMAKCCLEMEESLNAVYRQSRVSDKSIGPLE 540

Query: 541 IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPE 600
           IRVV+NGTFEELMD++ISRG+SINQYK PRCV+ TPI++LLDSRV S HFSPS PHW+PE
Sbjct: 541 IRVVQNGTFEELMDFSISRGSSINQYKVPRCVSLTPIMKLLDSRVVSAHFSPSLPHWSPE 596

BLAST of Spg013562 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 805.8 bits (2080), Expect = 2.3e-233
Identity = 385/594 (64.81%), Postives = 475/594 (79.97%), Query Frame = 0

Query: 17  DKDAKALRFIHDTTTNADSVQQRVLAEILTRNAHTEYVTRFRLNGATDRDTFKSKLPVVT 76
           +K+   L+FI D TTNAD VQ+RVL EIL+RNA  EY+ R  L G TDR+TFK  +PVVT
Sbjct: 19  EKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPVVT 78

Query: 77  YEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQLLYSLLM 136
           YED+QP+I RIANGD+S +  S+PISEFLTSSGTS GERKLMPTI+EE++RR LLYSLLM
Sbjct: 79  YEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLM 138

Query: 137 PIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFKTRPYDPFNDYTSP 196
           P+M+ +VPGLDKGKG+YFLF+K+E+KTPGGL ARPVLTSYYKS  FK RPYDP+ +YTSP
Sbjct: 139 PVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYTSP 198

Query: 197 NEAILCADSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAIHFLQLNWKQLAHDISTGT 256
           N+ ILC+DS+QSMY+QMLCGL    +VLRVGAVFASG +RAI FL+ +W +LA DI TGT
Sbjct: 199 NQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRTGT 258

Query: 257 LNPKITDPSLRECIISKYLTNPNPELAEFISKECSTEEWEGIVTRIWPNTKYLDVIVTGA 316
           L+ +ITD S+RE +    +  P+P+LA+F+  EC    W+GI+TR+WPNTKY+DVIVTG 
Sbjct: 259 LSSEITDSSVREAV--GEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGT 318

Query: 317 MAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEVTYTIMPNMGYFEFLP--- 376
           M+QYIPTL++YS GLP+ CTMYASSECYFGVNL+P+CKPSEV+YT++PNM YFEFLP   
Sbjct: 319 MSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHR 378

Query: 377 -----HDTSSPPALSRDSPPRLVDLADVEVGKEYELVITTYSGLCRYRVGDILQVTGFHN 436
                   S P AL+      LVDL DV++G+EYELV+TTY+GL RYRVGD+L V GF N
Sbjct: 379 NSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFKN 438

Query: 437 AAPQFRFVRRKNVLLSIDSDKTDEAELQKAIENASSLLREFNTSVVEYTSYADTKTIPGH 496
            APQF F+ RKNV+LSIDSDKTDE ELQ A++NA + L  F+ S+ EYTSYADT +IPGH
Sbjct: 439 NAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGH 498

Query: 497 YVIYWELRVKDAANSP-PEEILNQCCLAMEESLNSVYRQGRVADNSIGPLEIRVVKNGTF 556
           YV++WEL +    N+P P  +   CCL +EESLNSVYRQGRV+D SIGPLEI++V++GTF
Sbjct: 499 YVLFWELCLN--GNTPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESGTF 558

Query: 557 EELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPHWTPERRR 602
           ++LMDYAIS GASINQYK PRCV F PI+ELL+SRV   +FSP  P W+P  ++
Sbjct: 559 DKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSPGHKQ 608

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023543141.10.0e+0094.20probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita pepo subsp. pepo... [more]
KAG6604151.10.0e+0094.03putative indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma ... [more]
XP_022950930.10.0e+0093.86probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita moschata][more]
KAG7034313.10.0e+0093.68putative indole-3-acetic acid-amido synthetase GH3.1, partial [Cucurbita argyros... [more]
XP_022978925.10.0e+0093.53probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
O823335.5e-28077.91Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=... [more]
O221904.5e-26676.08Indole-3-acetic acid-amido synthetase GH3.3 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
A3BLS06.9e-25974.62Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=399... [more]
Q0D4Z66.9e-25974.62Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. japonica OX=3... [more]
P0C0M28.5e-25772.51Probable indole-3-acetic acid-amido synthetase GH3.2 OS=Oryza sativa subsp. japo... [more]
Match NameE-valueIdentityDescription
A0A6J1GH540.0e+0093.86probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita moschata OX=36... [more]
A0A6J1IRM50.0e+0093.53probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita maxima OX=3661... [more]
A0A5D3CNV80.0e+0093.19Putative indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo var. makuwa... [more]
A0A1S3B0G20.0e+0093.19probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo OX=3656 GN=... [more]
A0A0A0KJM50.0e+0092.86Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G493310 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G14960.13.9e-28177.91Auxin-responsive GH3 family protein [more]
AT4G37390.12.0e-26975.83Auxin-responsive GH3 family protein [more]
AT2G23170.13.2e-26776.08Auxin-responsive GH3 family protein [more]
AT1G59500.12.1e-25071.43Auxin-responsive GH3 family protein [more]
AT5G54510.12.3e-23364.81Auxin-responsive GH3 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004993GH3 familyPFAMPF03321GH3coord: 26..572
e-value: 9.4E-203
score: 674.8
IPR004993GH3 familyPANTHERPTHR31901GH3 DOMAIN-CONTAINING PROTEINcoord: 5..601
NoneNo IPR availablePANTHERPTHR31901:SF7INDOLE-3-ACETIC ACID-AMIDO SYNTHETASE GH3.2-RELATEDcoord: 5..601

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg013562.1Spg013562.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
molecular_function GO:0016881 acid-amino acid ligase activity