Spg013515 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg013515
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionsalicylic acid-binding protein 2-like
Locationscaffold1: 9156904 .. 9158414 (+)
RNA-Seq ExpressionSpg013515
SyntenySpg013515
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCTCAATCCCTCTCTCTCTTCATCTCTTAAACCTCATTTCTGATTAAATTAATCTAGACTTATATTCAAATGCATGAATTACATTTAATTACATACTTTGAATCTCTTTTTCTGAACAATCTCTTCTCTTCTCTCTCGATTTGTGTTGTGAGAGTCTACTACGTTACCAATAGTCCTTTACTGAAAGGAAAAAGAGGCCTGTAATCACATGGAGCAGAAGCATTTTGTGCTGGTTCATGGGGCTGGCCATGGAGCATGGACATGGTACAGGATCAAGCCGCTGCTCGAGGCGGCCAGCCACCGTGTAACCGTGCTGGACATGGTGGGGGCCGGGATCAACAGGAAGGCAATCCAGGAGGTTAAGTCGGTCGTAGAGTATGCAGAACCATTGTTGGAGACCATGGTAAGCGTTGGCCCAAATGAAAAGGTGATATTGGTGGGTCATAGCTTCGGTGGCATTAGTTTGGCTGTGGCCATGGAGAAGTTCCCTGAGAAGATATCAGCTTCTGTTTTCTTGACTGCCTTTGCTCCTGATACCCAACACAAGCCTTCTTATGTCGTAGATCGGGTACGTGTATGTTTGTATGCAAATATTCTCCTTTCGTAAATTAAAGTAAAGTATAATAAGAAGGGTTTTTTTAACATTTTTCATGGTGTTTGTTGATATGAAAGTTCATCGAGAGCGTTCCAAAGGAAATATGGGCGGATTCCCAGTTTTGTTCTCATGAAAACAGAGTTGATGGACCTTCAACTTCGTTCTCGTTTGGCCCCAAATGCCTGGCCGACAGTGTGTATCAACTCTCCTCTCCTGAGGTTCCCTCTATATATTTCTGATCTGTCTTTTTTTTTTTTTGTTGTTGTTCTTTCGAAGGGATTTTAATTTAATTTTTTTTTAACCACAATGATTAATGAAAATTAGGATGTAGCTTTGGCGAAGGCTCTGGTGAGACAAGCGTCACTGTTCCCAGAAGAACTGGGAAAAGCAGAGAACTTTACAGAGAAAAATTACGGATCAGTTAAAAAAGTGTACGTGGTTTGCAATGAAGACAAGCTTATCACGCCGGAGTTGCAGAAGTGGATAATTCAGAACTATGGGATTGAAAATGTGATGGAAATTGAAGGAGCTGATCACATGGCTATGTTTTCAAAGCCTCTCCAACTTTTCCAATGCCTTCTTCAGGTTGCTTGCAACTACACTTAAATATGAGCTCCCCTGTCCATTGGCCTTGTAATAATAAAAGCATTTGAATCTCTCACCACAACTGTTTACGTGTTAATTCGAGGTAGCTCACGACTACACTTAGATATGAGCTGCCCTCGTAATAATAGAAGTCCAAGACTGCATTTGAATCTCTAACCACTATTTACGTGTTTGATGGGATTTTCTACCAAAAATGTGTTTAATTGTATATTTGAAAAATTGGATGTGTTGTCTAATATATGTAGGTTGCTATTTGAGGTAGGTTGTAAAAAGGGAATCAATGATTGTGTATGTGTATGTGTATAAGTC

mRNA sequence

ATGGAGCAGAAGCATTTTGTGCTGGTTCATGGGGCTGGCCATGGAGCATGGACATGGTACAGGATCAAGCCGCTGCTCGAGGCGGCCAGCCACCGTGTAACCGTGCTGGACATGGTGGGGGCCGGGATCAACAGGAAGGCAATCCAGGAGGTTAAGTCGGTCGTAGAGTATGCAGAACCATTGTTGGAGACCATGGTAAGCGTTGGCCCAAATGAAAAGGTGATATTGGTGGGTCATAGCTTCGGTGGCATTAGTTTGGCTGTGGCCATGGAGAAGTTCCCTGAGAAGATATCAGCTTCTGTTTTCTTGACTGCCTTTGCTCCTGATACCCAACACAAGCCTTCTTATGTCGTAGATCGGTTCATCGAGAGCGTTCCAAAGGAAATATGGGCGGATTCCCAGTTTTGTTCTCATGAAAACAGAGTTGATGGACCTTCAACTTCGTTCTCGTTTGGCCCCAAATGCCTGGCCGACAGTGTGTATCAACTCTCCTCTCCTGAGGATGTAGCTTTGGCGAAGGCTCTGGTGAGACAAGCGTCACTGTTCCCAGAAGAACTGGGAAAAGCAGAGAACTTTACAGAGAAAAATTACGGATCAGTTAAAAAAGTGTACGTGGTTTGCAATGAAGACAAGCTTATCACGCCGGAGTTGCAGAAGTGGATAATTCAGAACTATGGGATTGAAAATGTGATGGAAATTGAAGGAGCTGATCACATGGCTATGTTTTCAAAGCCTCTCCAACTTTTCCAATGCCTTCTTCAGGTTGCTTGCAACTACACTTAA

Coding sequence (CDS)

ATGGAGCAGAAGCATTTTGTGCTGGTTCATGGGGCTGGCCATGGAGCATGGACATGGTACAGGATCAAGCCGCTGCTCGAGGCGGCCAGCCACCGTGTAACCGTGCTGGACATGGTGGGGGCCGGGATCAACAGGAAGGCAATCCAGGAGGTTAAGTCGGTCGTAGAGTATGCAGAACCATTGTTGGAGACCATGGTAAGCGTTGGCCCAAATGAAAAGGTGATATTGGTGGGTCATAGCTTCGGTGGCATTAGTTTGGCTGTGGCCATGGAGAAGTTCCCTGAGAAGATATCAGCTTCTGTTTTCTTGACTGCCTTTGCTCCTGATACCCAACACAAGCCTTCTTATGTCGTAGATCGGTTCATCGAGAGCGTTCCAAAGGAAATATGGGCGGATTCCCAGTTTTGTTCTCATGAAAACAGAGTTGATGGACCTTCAACTTCGTTCTCGTTTGGCCCCAAATGCCTGGCCGACAGTGTGTATCAACTCTCCTCTCCTGAGGATGTAGCTTTGGCGAAGGCTCTGGTGAGACAAGCGTCACTGTTCCCAGAAGAACTGGGAAAAGCAGAGAACTTTACAGAGAAAAATTACGGATCAGTTAAAAAAGTGTACGTGGTTTGCAATGAAGACAAGCTTATCACGCCGGAGTTGCAGAAGTGGATAATTCAGAACTATGGGATTGAAAATGTGATGGAAATTGAAGGAGCTGATCACATGGCTATGTTTTCAAAGCCTCTCCAACTTTTCCAATGCCTTCTTCAGGTTGCTTGCAACTACACTTAA

Protein sequence

MEQKHFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGINRKAIQEVKSVVEYAEPLLETMVSVGPNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVDRFIESVPKEIWADSQFCSHENRVDGPSTSFSFGPKCLADSVYQLSSPEDVALAKALVRQASLFPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQNYGIENVMEIEGADHMAMFSKPLQLFQCLLQVACNYT
Homology
BLAST of Spg013515 vs. NCBI nr
Match: XP_038881260.1 (salicylic acid-binding protein 2-like isoform X1 [Benincasa hispida])

HSP 1 Score: 396.4 bits (1017), Expect = 2.0e-106
Identity = 191/262 (72.90%), Postives = 220/262 (83.97%), Query Frame = 0

Query: 1   MEQKHFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGINRKAIQEVKSVVEYAEP 60
           MEQ+HFVLVHGA HGAW WY+I+PLLEAA HRVTVLDM GAG++RKAIQEVKS+ EY+EP
Sbjct: 1   MEQRHFVLVHGACHGAWCWYKIQPLLEAAGHRVTVLDMAGAGVHRKAIQEVKSLEEYSEP 60

Query: 61  LLETMVSVGPNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVDR 120
           LL+TM  VGPNEKVILVGHSFGG+SLA+AMEKFP  ISASVFLTA APDT H PSYV ++
Sbjct: 61  LLKTMACVGPNEKVILVGHSFGGMSLALAMEKFPHNISASVFLTALAPDTHHHPSYVSEQ 120

Query: 121 FIESVPKEIWADSQFCSHENRVDGPSTS--FSFGPKCLADSVYQLSSPEDVALAKALVRQ 180
           F+ES+PKE W D+Q C +ENRVD  ++S  F FGPKC+A+ +Y LS  ED+AL K+LVR 
Sbjct: 121 FLESLPKEFWMDTQSCGYENRVDDEASSSYFLFGPKCMANFIYHLSPSEDLALGKSLVRP 180

Query: 181 ASLFPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQNYGIENVMEIEGADH 240
            SL  EEL KAE FTE+NYGSVKKVYV+C EDK I    QKW+IQNYGI+NVMEIEGADH
Sbjct: 181 GSLLVEELAKAEKFTEENYGSVKKVYVICGEDKTIPKHFQKWMIQNYGIQNVMEIEGADH 240

Query: 241 MAMFSKPLQLFQCLLQVACNYT 261
           M MFSKPLQ+ QCLLQVA N+T
Sbjct: 241 MPMFSKPLQVLQCLLQVAHNFT 262

BLAST of Spg013515 vs. NCBI nr
Match: XP_011657843.2 (salicylic acid-binding protein 2 [Cucumis sativus] >KAE8647492.1 hypothetical protein Csa_003158 [Cucumis sativus])

HSP 1 Score: 374.8 bits (961), Expect = 6.1e-100
Identity = 184/262 (70.23%), Postives = 218/262 (83.21%), Query Frame = 0

Query: 2   EQKHFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGINRKAIQEVKSVVEYAEPL 61
           + KHFVLVHGA HGAW WY+IKPLLEAA HRVT+LDM GAG+NRKAIQEV+S  EY+EPL
Sbjct: 3   QMKHFVLVHGACHGAWCWYKIKPLLEAAGHRVTMLDMGGAGVNRKAIQEVESFEEYSEPL 62

Query: 62  LETMVSVGPNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVDRF 121
           L+TM  +GPNEKVILVGHSFGG+SLA+AME FP KISASVF+TAF PDT H PSYV+++F
Sbjct: 63  LKTMACLGPNEKVILVGHSFGGMSLALAMENFPHKISASVFITAFVPDTHHPPSYVLEQF 122

Query: 122 IESVPKEIWADSQFCSHENRVDGPSTS-FSFGPKCLADSVYQLSSPEDVALAKALVRQAS 181
           +ES+P+E W D++F   ENR DG S+S F FGPKC+A+ +YQ S  ED AL  +LVR A 
Sbjct: 123 LESLPREFWMDTEF--GENREDGGSSSWFLFGPKCMANKIYQFSPTEDQALGSSLVRPAK 182

Query: 182 LFPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQN--YGIENVMEIEGADH 241
           LF E LGKAE FTE+NYGSVKKVYV+C ED+ I+ +LQKW+IQN   GI+NVMEI+ ADH
Sbjct: 183 LFIENLGKAEKFTEENYGSVKKVYVICGEDRTISKQLQKWMIQNSGKGIQNVMEIDEADH 242

Query: 242 MAMFSKPLQLFQCLLQVACNYT 261
           MAMFSKPLQ+ QCLLQVA +YT
Sbjct: 243 MAMFSKPLQVLQCLLQVAQSYT 262

BLAST of Spg013515 vs. NCBI nr
Match: XP_022977269.1 (salicylic acid-binding protein 2-like [Cucurbita maxima])

HSP 1 Score: 372.1 bits (954), Expect = 4.0e-99
Identity = 181/258 (70.16%), Postives = 216/258 (83.72%), Query Frame = 0

Query: 1   MEQKHFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGINRKAIQEVKSVVEYAEP 60
           MEQKHFVLVHGA HGAWTWY+IKPLLEAA HRVTVLDM GAG+N KAIQEVKS VEY+EP
Sbjct: 46  MEQKHFVLVHGACHGAWTWYKIKPLLEAAGHRVTVLDMAGAGVNMKAIQEVKSFVEYSEP 105

Query: 61  LLETMVSVGPNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVDR 120
           LL+ +  VGPNEKVILVGHS GG+SLAVAMEKFP KI+ASVF+TAF PDT H+PS V+++
Sbjct: 106 LLQALSCVGPNEKVILVGHSLGGMSLAVAMEKFPHKIAASVFVTAFVPDTHHQPSDVIEQ 165

Query: 121 FIESVPKEIWADSQFCSHENRVDGPSTSFSFGPKCLADSVYQLSSPEDVALAKALVRQAS 180
           F ES+PKE W D+Q  ++    D PS+ F FGPKC A+++YQLS  ED+ALA  L+R  S
Sbjct: 166 FRESLPKEFWQDTQVSTN----DKPSSWFIFGPKCTANNIYQLSPSEDLALAMTLMRPGS 225

Query: 181 LFPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQNYGIENVMEIEGADHMA 240
           LF E+L  AE FTEKNYGSVKKVYV+C+EDK I+ + QK +I+++GI++VMEI+GADHM 
Sbjct: 226 LFKEDLANAEKFTEKNYGSVKKVYVICSEDKTISKQFQKRMIESHGIQDVMEIDGADHMP 285

Query: 241 MFSKPLQLFQCLLQVACN 259
           MFSKPLQLF+CLLQVA N
Sbjct: 286 MFSKPLQLFKCLLQVAHN 299

BLAST of Spg013515 vs. NCBI nr
Match: XP_022949785.1 (salicylic acid-binding protein 2-like [Cucurbita moschata])

HSP 1 Score: 368.2 bits (944), Expect = 5.7e-98
Identity = 180/260 (69.23%), Postives = 215/260 (82.69%), Query Frame = 0

Query: 1   MEQKHFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGINRKAIQEVKSVVEYAEP 60
           MEQKHFV+VHGA HGAWTWY+IKPLLEAA HRV VLDM GAG+N KAIQEVKS VEY+EP
Sbjct: 23  MEQKHFVMVHGACHGAWTWYKIKPLLEAAGHRVMVLDMAGAGVNMKAIQEVKSFVEYSEP 82

Query: 61  LLETMVSVGPNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVDR 120
           LL+ M  VGPNEKVILVGHS GG+SLAVAMEKFP KI+ASVF+TAF PDT H+PS V+++
Sbjct: 83  LLQAMSCVGPNEKVILVGHSLGGMSLAVAMEKFPHKIAASVFVTAFVPDTHHQPSDVIEQ 142

Query: 121 FIESVPKEIWADSQFCSHENRVDGPSTSFSFGPKCLADSVYQLSSPEDVALAKALVRQAS 180
           F ES+P E W D+Q  ++    D PS+ F FGPKC A+++YQLS  ED+ALA  L+R  S
Sbjct: 143 FRESLPMEFWQDTQVPTN----DKPSSWFIFGPKCTANNIYQLSPSEDLALAMTLMRPGS 202

Query: 181 LFPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQNYGIENVMEIEGADHMA 240
           LF E+L  AE FTE+NYGSVKKVYV+C+EDK I+ + QK +I+NYGI++VMEI+GADHM 
Sbjct: 203 LFKEDLANAEKFTEENYGSVKKVYVMCSEDKTISKQFQKRMIENYGIQDVMEIDGADHMP 262

Query: 241 MFSKPLQLFQCLLQVACNYT 261
           MFSKPLQLF+CLL+VA N T
Sbjct: 263 MFSKPLQLFKCLLKVAHNCT 278

BLAST of Spg013515 vs. NCBI nr
Match: KAG6604201.1 (Salicylic acid-binding protein 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 365.9 bits (938), Expect = 2.8e-97
Identity = 179/260 (68.85%), Postives = 214/260 (82.31%), Query Frame = 0

Query: 1   MEQKHFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGINRKAIQEVKSVVEYAEP 60
           MEQKHFV+VHGA HGAWTWY+IKPLLEAA HRVTVLDM GAG+N KAIQEVKS VEY+EP
Sbjct: 1   MEQKHFVMVHGACHGAWTWYKIKPLLEAAGHRVTVLDMAGAGVNMKAIQEVKSFVEYSEP 60

Query: 61  LLETMVSVGPNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVDR 120
           LL+ M  V P EKVILVGHS GG+SLAVAMEKFP KI+ASVF+TAF PDT H+PS V+++
Sbjct: 61  LLQAMSCVDPKEKVILVGHSLGGMSLAVAMEKFPHKIAASVFVTAFVPDTHHQPSAVIEQ 120

Query: 121 FIESVPKEIWADSQFCSHENRVDGPSTSFSFGPKCLADSVYQLSSPEDVALAKALVRQAS 180
           F ES+P E W D+Q  ++    D PS+ F FGPKC A+++YQLS  ED+ALA  L+R  S
Sbjct: 121 FRESLPMEFWQDTQVPTN----DKPSSWFIFGPKCTANNIYQLSPSEDLALAMTLMRPGS 180

Query: 181 LFPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQNYGIENVMEIEGADHMA 240
           LF E+L  AE FTE+NYGSVKKVYV+C+EDK I+ + QK +I+NYGI++VMEI+GADHM 
Sbjct: 181 LFKEDLANAEKFTEENYGSVKKVYVMCSEDKTISKQFQKRMIENYGIQDVMEIDGADHMP 240

Query: 241 MFSKPLQLFQCLLQVACNYT 261
           MFSKPLQLF+CLL+VA N T
Sbjct: 241 MFSKPLQLFKCLLKVAHNCT 256

BLAST of Spg013515 vs. ExPASy Swiss-Prot
Match: Q6RYA0 (Salicylic acid-binding protein 2 OS=Nicotiana tabacum OX=4097 GN=SABP2 PE=1 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 1.7e-76
Identity = 137/258 (53.10%), Postives = 188/258 (72.87%), Query Frame = 0

Query: 2   EQKHFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGINRKAIQEVKSVVEYAEPL 61
           E KHFVLVHGA HG W+WY++KPLLEAA H+VT LD+  +G + + I+E++++ +Y  PL
Sbjct: 3   EGKHFVLVHGACHGGWSWYKLKPLLEAAGHKVTALDLAASGTDLRKIEELRTLYDYTLPL 62

Query: 62  LETMVSVGPNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVDRF 121
           +E M S+  +EKVILVGHS GG++L +AMEK+P+KI A+VFL AF PD+ H  S+V++++
Sbjct: 63  MELMESLSADEKVILVGHSLGGMNLGLAMEKYPQKIYAAVFLAAFMPDSVHNSSFVLEQY 122

Query: 122 IESVPKEIWADSQFCSHENRVDGPSTSFSFGPKCLADSVYQLSSPEDVALAKALVRQASL 181
            E  P E W D+QF  +    + P TS  FGPK LA  +YQL SPED+ALA +LVR +SL
Sbjct: 123 NERTPAENWLDTQFLPY-GSPEEPLTSMFFGPKFLAHKLYQLCSPEDLALASSLVRPSSL 182

Query: 182 FPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQNYGIENVMEIEGADHMAM 241
           F E+L KA+ FT++ +GSVK+VY+VC EDK I  E Q+W I N G+   +EI+GADHMAM
Sbjct: 183 FMEDLSKAKYFTDERFGSVKRVYIVCTEDKGIPEEFQRWQIDNIGVTEAIEIKGADHMAM 242

Query: 242 FSKPLQLFQCLLQVACNY 260
             +P +L   LL++A  Y
Sbjct: 243 LCEPQKLCASLLEIAHKY 259

BLAST of Spg013515 vs. ExPASy Swiss-Prot
Match: Q8S8S9 (Methylesterase 1 OS=Arabidopsis thaliana OX=3702 GN=MES1 PE=1 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 2.0e-69
Identity = 125/255 (49.02%), Postives = 180/255 (70.59%), Query Frame = 0

Query: 3   QKHFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGIN-RKAIQEVKSVVEYAEPL 62
           ++HFVLVHG+ HGAW WY++KPLLEA  HRVT +D+  +GI+  ++I ++ +  +Y+EPL
Sbjct: 7   KQHFVLVHGSCHGAWCWYKVKPLLEAVGHRVTAVDLAASGIDTTRSITDIPTCEQYSEPL 66

Query: 63  LETMVSVGPNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVDRF 122
            + + S+  +EKV+LVGHSFGG++LA+AMEKFPEKIS +VFLTAF PDT+H PS+V+D+F
Sbjct: 67  TKLLTSLPNDEKVVLVGHSFGGLNLAIAMEKFPEKISVAVFLTAFMPDTEHSPSFVLDKF 126

Query: 123 IESVPKEIWADSQFCSHENRVDGPSTSFSFGPKCLADSVYQLSSPEDVALAKALVRQASL 182
             ++P+E W  ++F  + +  D    S  F P  +   +YQLS  ED+ L   L+R  SL
Sbjct: 127 GSNMPQEAWMGTEFEPYGS--DNSGLSMFFSPDFMKLGLYQLSPVEDLELGLLLMRPGSL 186

Query: 183 FPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQNYGIENVMEIEGADHMAM 242
           F  +L K +NF+++ YGSV +V++VC EDK I  E Q+W+I N+ +  VME+E  DHM M
Sbjct: 187 FINDLSKMKNFSDEGYGSVPRVFIVCKEDKAIPEERQRWMIDNFPVNLVMEMEETDHMPM 246

Query: 243 FSKPLQLFQCLLQVA 257
           F KP QL    L++A
Sbjct: 247 FCKPQQLSDYFLKIA 259

BLAST of Spg013515 vs. ExPASy Swiss-Prot
Match: Q9SE93 (Polyneuridine-aldehyde esterase OS=Rauvolfia serpentina OX=4060 GN=PNAE PE=1 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 4.4e-69
Identity = 121/255 (47.45%), Postives = 177/255 (69.41%), Query Frame = 0

Query: 2   EQKHFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGINRKAIQEVKSVVEYAEPL 61
           +QKHFVLVHG   GAW WY++KPLLE+A H+VT +D+  AGIN + + E+ +  +Y+EPL
Sbjct: 9   QQKHFVLVHGGCLGAWIWYKLKPLLESAGHKVTAVDLSAAGINPRRLDEIHTFRDYSEPL 68

Query: 62  LETMVSVGPNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVDRF 121
           +E M S+ P+EKV+L+GHSFGG+SL +AME +PEKIS +VF++A  PD  H  +Y  +++
Sbjct: 69  MEVMASIPPDEKVVLLGHSFGGMSLGLAMETYPEKISVAVFMSAMMPDPNHSLTYPFEKY 128

Query: 122 IESVPKEIWADSQFCSHENRVDGPSTSFSFGPKCLADSVYQLSSPEDVALAKALVRQASL 181
            E  P ++  DSQF ++ N  + P  S   GP+ +A  ++Q  S ED+ LAK L R  SL
Sbjct: 129 NEKCPADMMLDSQFSTYGN-PENPGMSMILGPQFMALKMFQNCSVEDLELAKMLTRPGSL 188

Query: 182 FPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQNYGIENVMEIEGADHMAM 241
           F ++L KA+ F+ + YGSVK+ Y+ CNEDK    E QKW +++ G + V EI+ ADHM M
Sbjct: 189 FFQDLAKAKKFSTERYGSVKRAYIFCNEDKSFPVEFQKWFVESVGADKVKEIKEADHMGM 248

Query: 242 FSKPLQLFQCLLQVA 257
            S+P ++ +CLL ++
Sbjct: 249 LSQPREVCKCLLDIS 262

BLAST of Spg013515 vs. ExPASy Swiss-Prot
Match: O80476 (Methylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=MES2 PE=1 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 2.9e-68
Identity = 128/258 (49.61%), Postives = 177/258 (68.60%), Query Frame = 0

Query: 3   QKHFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGIN-RKAIQEVKSVVEYAEPL 62
           ++HFVLVHGA HGAW WY++KPLLEA  HRVT LD+  +GI+  ++I ++ +  +Y+EPL
Sbjct: 7   KQHFVLVHGACHGAWCWYKVKPLLEALGHRVTALDLAASGIDTTRSITDISTCEQYSEPL 66

Query: 63  LETMVSVGPNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVDRF 122
           ++ M S+  +EKV+LVGHSFGG+SLA+AM+KFP+KIS SVF+TAF PDT+H PS+V ++F
Sbjct: 67  MQLMTSLPNDEKVVLVGHSFGGLSLALAMDKFPDKISVSVFVTAFMPDTKHSPSFVEEKF 126

Query: 123 IESVPKEIWADSQFCSHENRVDGPSTSFSFGPKCLADSVYQLSSPEDVALAKALVRQASL 182
             S+  E W  S+  ++ +  D    S  F    +   +YQLS  ED+ L   L R +SL
Sbjct: 127 ASSMTPEGWMGSELETYGS--DNSGLSVFFSTDFMKHRLYQLSPVEDLELGLLLKRPSSL 186

Query: 183 FPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQNYGIENVMEIEGADHMAM 242
           F  EL K ENF+EK YGSV + Y+VC ED +I+ + Q+W+I NY    V+E+E  DHM M
Sbjct: 187 FINELSKMENFSEKGYGSVPRAYIVCKEDNIISEDHQRWMIHNYPANLVIEMEETDHMPM 246

Query: 243 FSKPLQLFQCLLQVACNY 260
           F KP  L   LL +A N+
Sbjct: 247 FCKPQLLSDHLLAIADNF 262

BLAST of Spg013515 vs. ExPASy Swiss-Prot
Match: Q9LFT6 (Alpha-hydroxynitrile lyase OS=Arabidopsis thaliana OX=3702 GN=HNL PE=1 SV=1)

HSP 1 Score: 255.8 bits (652), Expect = 5.4e-67
Identity = 130/260 (50.00%), Postives = 180/260 (69.23%), Query Frame = 0

Query: 1   MEQK-HFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGINRKAIQEVKSVVEYAE 60
           ME+K HFVLVH A HGAW WY++KPLLE+A HRVT +++  +GI+ + IQ V++V EY++
Sbjct: 1   MERKHHFVLVHNAYHGAWIWYKLKPLLESAGHRVTAVELAASGIDPRPIQAVETVDEYSK 60

Query: 61  PLLETMVSVGPNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVD 120
           PL+ET+ S+  NE+VILVG SFGGI++A+A + FP KI   VFL AF PDT H PS+V+D
Sbjct: 61  PLIETLKSLPENEEVILVGFSFGGINIALAADIFPAKIKVLVFLNAFLPDTTHVPSHVLD 120

Query: 121 RFIESVPKEIWADSQFCSHENRVDGPSTSFSFGPKCLADSVYQLSSPEDVALAKALVRQA 180
           +++E +P  +  D +F SHE R +G  +    GPK +   +YQ    ED  LAK L RQ 
Sbjct: 121 KYME-MPGGL-GDCEFSSHETR-NGTMSLLKMGPKFMKARLYQNCPIEDYELAKMLHRQG 180

Query: 181 SLFPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQNYGIENVMEIEGADHM 240
           S F E+L K E F+E+ YGSV++VYV+ +EDK I  +  +W+I N+ +  V EI+G DHM
Sbjct: 181 SFFTEDLSKKEKFSEEGYGSVQRVYVMSSEDKAIPCDFIRWMIDNFNVSKVYEIDGGDHM 240

Query: 241 AMFSKPLQLFQCLLQVACNY 260
            M SKP +LF  L  +A +Y
Sbjct: 241 VMLSKPQKLFDSLSAIATDY 257

BLAST of Spg013515 vs. ExPASy TrEMBL
Match: A0A0A0KG72 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G490080 PE=4 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 6.6e-100
Identity = 183/262 (69.85%), Postives = 218/262 (83.21%), Query Frame = 0

Query: 2   EQKHFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGINRKAIQEVKSVVEYAEPL 61
           + KHFVLVHGA HGAW WY+IKPLLEAA HRVT+LDM GAG+NR+AIQEV+S  EY+EPL
Sbjct: 3   QMKHFVLVHGACHGAWCWYKIKPLLEAAGHRVTMLDMGGAGVNRRAIQEVESFEEYSEPL 62

Query: 62  LETMVSVGPNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVDRF 121
           L+TM  +GPNEKVILVGHSFGG+SLA+AME FP KISASVF+TAF PDT H PSYV+++F
Sbjct: 63  LKTMACLGPNEKVILVGHSFGGMSLALAMENFPHKISASVFITAFVPDTHHPPSYVLEQF 122

Query: 122 IESVPKEIWADSQFCSHENRVDGPSTS-FSFGPKCLADSVYQLSSPEDVALAKALVRQAS 181
           +ES+P+E W D++F   ENR DG S+S F FGPKC+A+ +YQ S  ED AL  +LVR A 
Sbjct: 123 LESLPREFWMDTEF--GENREDGGSSSWFLFGPKCMANKIYQFSPTEDQALGSSLVRPAK 182

Query: 182 LFPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQN--YGIENVMEIEGADH 241
           LF E LGKAE FTE+NYGSVKKVYV+C ED+ I+ +LQKW+IQN   GI+NVMEI+ ADH
Sbjct: 183 LFIENLGKAEKFTEENYGSVKKVYVICGEDRTISKQLQKWMIQNSGKGIQNVMEIDEADH 242

Query: 242 MAMFSKPLQLFQCLLQVACNYT 261
           MAMFSKPLQ+ QCLLQVA +YT
Sbjct: 243 MAMFSKPLQVLQCLLQVAQSYT 262

BLAST of Spg013515 vs. ExPASy TrEMBL
Match: A0A6J1IQY1 (salicylic acid-binding protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111477637 PE=4 SV=1)

HSP 1 Score: 372.1 bits (954), Expect = 1.9e-99
Identity = 181/258 (70.16%), Postives = 216/258 (83.72%), Query Frame = 0

Query: 1   MEQKHFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGINRKAIQEVKSVVEYAEP 60
           MEQKHFVLVHGA HGAWTWY+IKPLLEAA HRVTVLDM GAG+N KAIQEVKS VEY+EP
Sbjct: 46  MEQKHFVLVHGACHGAWTWYKIKPLLEAAGHRVTVLDMAGAGVNMKAIQEVKSFVEYSEP 105

Query: 61  LLETMVSVGPNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVDR 120
           LL+ +  VGPNEKVILVGHS GG+SLAVAMEKFP KI+ASVF+TAF PDT H+PS V+++
Sbjct: 106 LLQALSCVGPNEKVILVGHSLGGMSLAVAMEKFPHKIAASVFVTAFVPDTHHQPSDVIEQ 165

Query: 121 FIESVPKEIWADSQFCSHENRVDGPSTSFSFGPKCLADSVYQLSSPEDVALAKALVRQAS 180
           F ES+PKE W D+Q  ++    D PS+ F FGPKC A+++YQLS  ED+ALA  L+R  S
Sbjct: 166 FRESLPKEFWQDTQVSTN----DKPSSWFIFGPKCTANNIYQLSPSEDLALAMTLMRPGS 225

Query: 181 LFPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQNYGIENVMEIEGADHMA 240
           LF E+L  AE FTEKNYGSVKKVYV+C+EDK I+ + QK +I+++GI++VMEI+GADHM 
Sbjct: 226 LFKEDLANAEKFTEKNYGSVKKVYVICSEDKTISKQFQKRMIESHGIQDVMEIDGADHMP 285

Query: 241 MFSKPLQLFQCLLQVACN 259
           MFSKPLQLF+CLLQVA N
Sbjct: 286 MFSKPLQLFKCLLQVAHN 299

BLAST of Spg013515 vs. ExPASy TrEMBL
Match: A0A6J1GDS1 (salicylic acid-binding protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111453076 PE=4 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 2.8e-98
Identity = 180/260 (69.23%), Postives = 215/260 (82.69%), Query Frame = 0

Query: 1   MEQKHFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGINRKAIQEVKSVVEYAEP 60
           MEQKHFV+VHGA HGAWTWY+IKPLLEAA HRV VLDM GAG+N KAIQEVKS VEY+EP
Sbjct: 23  MEQKHFVMVHGACHGAWTWYKIKPLLEAAGHRVMVLDMAGAGVNMKAIQEVKSFVEYSEP 82

Query: 61  LLETMVSVGPNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVDR 120
           LL+ M  VGPNEKVILVGHS GG+SLAVAMEKFP KI+ASVF+TAF PDT H+PS V+++
Sbjct: 83  LLQAMSCVGPNEKVILVGHSLGGMSLAVAMEKFPHKIAASVFVTAFVPDTHHQPSDVIEQ 142

Query: 121 FIESVPKEIWADSQFCSHENRVDGPSTSFSFGPKCLADSVYQLSSPEDVALAKALVRQAS 180
           F ES+P E W D+Q  ++    D PS+ F FGPKC A+++YQLS  ED+ALA  L+R  S
Sbjct: 143 FRESLPMEFWQDTQVPTN----DKPSSWFIFGPKCTANNIYQLSPSEDLALAMTLMRPGS 202

Query: 181 LFPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQNYGIENVMEIEGADHMA 240
           LF E+L  AE FTE+NYGSVKKVYV+C+EDK I+ + QK +I+NYGI++VMEI+GADHM 
Sbjct: 203 LFKEDLANAEKFTEENYGSVKKVYVMCSEDKTISKQFQKRMIENYGIQDVMEIDGADHMP 262

Query: 241 MFSKPLQLFQCLLQVACNYT 261
           MFSKPLQLF+CLL+VA N T
Sbjct: 263 MFSKPLQLFKCLLKVAHNCT 278

BLAST of Spg013515 vs. ExPASy TrEMBL
Match: A0A6J1HDY0 (salicylic acid-binding protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111463111 PE=4 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 3.6e-90
Identity = 164/256 (64.06%), Postives = 200/256 (78.12%), Query Frame = 0

Query: 1   MEQKHFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGINRKAIQEVKSVVEYAEP 60
           MEQKHFV VHGA HGAW+W++IKPLLEAA HRVT+LDM  +GI+RK IQ+V S+V+Y+EP
Sbjct: 27  MEQKHFVFVHGACHGAWSWFKIKPLLEAAGHRVTLLDMAASGIHRKVIQDVHSIVDYSEP 86

Query: 61  LLETMVSVGPNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVDR 120
           LL+ M  + PN+KVILVGHS GG +LAVAME+FP+KI+ASVF+ AF PDT HKPSYV+D+
Sbjct: 87  LLKVMACLPPNQKVILVGHSLGGFNLAVAMERFPDKIAASVFVAAFVPDTDHKPSYVLDQ 146

Query: 121 FIESVPKEIWADSQFCSHENRVDGPSTSFSFGPKCLADSVYQLSSPEDVALAKALVRQAS 180
           + E  P E W D+QF  + N    PSTS  FGP  +A  +YQLS PED+AL  +LVR +S
Sbjct: 147 YNEQTPMEAWLDTQFSPYGNEAK-PSTSMFFGPDFVAKKLYQLSPPEDLALGLSLVRPSS 206

Query: 181 LFPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQNYGIENVMEIEGADHMA 240
           LF ++L KA NF+++NYGSVKKVY+VC ED  I  E QKW+I N GIENVMEI G+DHM 
Sbjct: 207 LFMQDLSKANNFSKQNYGSVKKVYIVCTEDIGIPEEFQKWMIHNGGIENVMEINGSDHMP 266

Query: 241 MFSKPLQLFQCLLQVA 257
           MF KP QL  CLLQVA
Sbjct: 267 MFCKPHQLLHCLLQVA 281

BLAST of Spg013515 vs. ExPASy TrEMBL
Match: A0A6J1KUH6 (salicylic acid-binding protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111497310 PE=4 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 4.7e-90
Identity = 162/256 (63.28%), Postives = 200/256 (78.12%), Query Frame = 0

Query: 1   MEQKHFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGINRKAIQEVKSVVEYAEP 60
           MEQKHFV VHGA HGAW+W++IKPLLEAA HRVT+LDM  +GI+RK IQ+V S+V+Y+EP
Sbjct: 1   MEQKHFVFVHGACHGAWSWFKIKPLLEAAGHRVTLLDMAASGIHRKVIQDVHSIVDYSEP 60

Query: 61  LLETMVSVGPNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVDR 120
           LL+ M  + PN+KVILVGHS GG +LAVAME+FP+KI+ASVF+ AF PDT HKPSYV+D+
Sbjct: 61  LLKAMACLPPNQKVILVGHSLGGFNLAVAMERFPDKIAASVFVAAFVPDTNHKPSYVLDQ 120

Query: 121 FIESVPKEIWADSQFCSHENRVDGPSTSFSFGPKCLADSVYQLSSPEDVALAKALVRQAS 180
           + E  P E W D+QF  +    + PSTS  FGP  +A  +YQLS PED+AL  +L+R +S
Sbjct: 121 YNEQTPLEAWLDTQFSPYGTEAE-PSTSMFFGPNFVAKKLYQLSPPEDLALGLSLLRPSS 180

Query: 181 LFPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQNYGIENVMEIEGADHMA 240
           LF ++L KA NF+++ YGSVKKVY+VC EDK I  E QKW+I N GIENVMEI G+DHM 
Sbjct: 181 LFMQDLSKANNFSKQKYGSVKKVYIVCTEDKGIPEEFQKWMIHNVGIENVMEINGSDHMP 240

Query: 241 MFSKPLQLFQCLLQVA 257
           MF KP QL  CLLQVA
Sbjct: 241 MFCKPRQLLHCLLQVA 255

BLAST of Spg013515 vs. TAIR 10
Match: AT2G23620.1 (methyl esterase 1 )

HSP 1 Score: 263.8 bits (673), Expect = 1.4e-70
Identity = 125/255 (49.02%), Postives = 180/255 (70.59%), Query Frame = 0

Query: 3   QKHFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGIN-RKAIQEVKSVVEYAEPL 62
           ++HFVLVHG+ HGAW WY++KPLLEA  HRVT +D+  +GI+  ++I ++ +  +Y+EPL
Sbjct: 7   KQHFVLVHGSCHGAWCWYKVKPLLEAVGHRVTAVDLAASGIDTTRSITDIPTCEQYSEPL 66

Query: 63  LETMVSVGPNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVDRF 122
            + + S+  +EKV+LVGHSFGG++LA+AMEKFPEKIS +VFLTAF PDT+H PS+V+D+F
Sbjct: 67  TKLLTSLPNDEKVVLVGHSFGGLNLAIAMEKFPEKISVAVFLTAFMPDTEHSPSFVLDKF 126

Query: 123 IESVPKEIWADSQFCSHENRVDGPSTSFSFGPKCLADSVYQLSSPEDVALAKALVRQASL 182
             ++P+E W  ++F  + +  D    S  F P  +   +YQLS  ED+ L   L+R  SL
Sbjct: 127 GSNMPQEAWMGTEFEPYGS--DNSGLSMFFSPDFMKLGLYQLSPVEDLELGLLLMRPGSL 186

Query: 183 FPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQNYGIENVMEIEGADHMAM 242
           F  +L K +NF+++ YGSV +V++VC EDK I  E Q+W+I N+ +  VME+E  DHM M
Sbjct: 187 FINDLSKMKNFSDEGYGSVPRVFIVCKEDKAIPEERQRWMIDNFPVNLVMEMEETDHMPM 246

Query: 243 FSKPLQLFQCLLQVA 257
           F KP QL    L++A
Sbjct: 247 FCKPQQLSDYFLKIA 259

BLAST of Spg013515 vs. TAIR 10
Match: AT2G23600.1 (acetone-cyanohydrin lyase )

HSP 1 Score: 260.0 bits (663), Expect = 2.0e-69
Identity = 128/258 (49.61%), Postives = 177/258 (68.60%), Query Frame = 0

Query: 3   QKHFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGIN-RKAIQEVKSVVEYAEPL 62
           ++HFVLVHGA HGAW WY++KPLLEA  HRVT LD+  +GI+  ++I ++ +  +Y+EPL
Sbjct: 7   KQHFVLVHGACHGAWCWYKVKPLLEALGHRVTALDLAASGIDTTRSITDISTCEQYSEPL 66

Query: 63  LETMVSVGPNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVDRF 122
           ++ M S+  +EKV+LVGHSFGG+SLA+AM+KFP+KIS SVF+TAF PDT+H PS+V ++F
Sbjct: 67  MQLMTSLPNDEKVVLVGHSFGGLSLALAMDKFPDKISVSVFVTAFMPDTKHSPSFVEEKF 126

Query: 123 IESVPKEIWADSQFCSHENRVDGPSTSFSFGPKCLADSVYQLSSPEDVALAKALVRQASL 182
             S+  E W  S+  ++ +  D    S  F    +   +YQLS  ED+ L   L R +SL
Sbjct: 127 ASSMTPEGWMGSELETYGS--DNSGLSVFFSTDFMKHRLYQLSPVEDLELGLLLKRPSSL 186

Query: 183 FPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQNYGIENVMEIEGADHMAM 242
           F  EL K ENF+EK YGSV + Y+VC ED +I+ + Q+W+I NY    V+E+E  DHM M
Sbjct: 187 FINELSKMENFSEKGYGSVPRAYIVCKEDNIISEDHQRWMIHNYPANLVIEMEETDHMPM 246

Query: 243 FSKPLQLFQCLLQVACNY 260
           F KP  L   LL +A N+
Sbjct: 247 FCKPQLLSDHLLAIADNF 262

BLAST of Spg013515 vs. TAIR 10
Match: AT5G10300.1 (methyl esterase 5 )

HSP 1 Score: 255.8 bits (652), Expect = 3.8e-68
Identity = 130/260 (50.00%), Postives = 180/260 (69.23%), Query Frame = 0

Query: 1   MEQK-HFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGINRKAIQEVKSVVEYAE 60
           ME+K HFVLVH A HGAW WY++KPLLE+A HRVT +++  +GI+ + IQ V++V EY++
Sbjct: 1   MERKHHFVLVHNAYHGAWIWYKLKPLLESAGHRVTAVELAASGIDPRPIQAVETVDEYSK 60

Query: 61  PLLETMVSVGPNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVD 120
           PL+ET+ S+  NE+VILVG SFGGI++A+A + FP KI   VFL AF PDT H PS+V+D
Sbjct: 61  PLIETLKSLPENEEVILVGFSFGGINIALAADIFPAKIKVLVFLNAFLPDTTHVPSHVLD 120

Query: 121 RFIESVPKEIWADSQFCSHENRVDGPSTSFSFGPKCLADSVYQLSSPEDVALAKALVRQA 180
           +++E +P  +  D +F SHE R +G  +    GPK +   +YQ    ED  LAK L RQ 
Sbjct: 121 KYME-MPGGL-GDCEFSSHETR-NGTMSLLKMGPKFMKARLYQNCPIEDYELAKMLHRQG 180

Query: 181 SLFPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQNYGIENVMEIEGADHM 240
           S F E+L K E F+E+ YGSV++VYV+ +EDK I  +  +W+I N+ +  V EI+G DHM
Sbjct: 181 SFFTEDLSKKEKFSEEGYGSVQRVYVMSSEDKAIPCDFIRWMIDNFNVSKVYEIDGGDHM 240

Query: 241 AMFSKPLQLFQCLLQVACNY 260
            M SKP +LF  L  +A +Y
Sbjct: 241 VMLSKPQKLFDSLSAIATDY 257

BLAST of Spg013515 vs. TAIR 10
Match: AT2G23610.1 (methyl esterase 3 )

HSP 1 Score: 249.2 bits (635), Expect = 3.6e-66
Identity = 120/258 (46.51%), Postives = 180/258 (69.77%), Query Frame = 0

Query: 3   QKHFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGIN-RKAIQEVKSVVEYAEPL 62
           ++H VLVHGA HGAW WY++KP LEA+ HRVT +D+  +GI+  ++I ++ +  +Y+EPL
Sbjct: 7   KQHVVLVHGACHGAWCWYKVKPQLEASGHRVTAVDLAASGIDMTRSITDISTCEQYSEPL 66

Query: 63  LETMVSVGPNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVDRF 122
           ++ M S+  +EKV+LVGHS GG+SLA+AM+ FP KIS SVF+TA  PDT+H PS+V D+ 
Sbjct: 67  MQLMTSLPDDEKVVLVGHSLGGLSLAMAMDMFPTKISVSVFVTAMMPDTKHSPSFVWDKL 126

Query: 123 IESVPKEIWADSQFCSHENRVDGPSTSFSFGPKCLADSVYQLSSPEDVALAKALVRQASL 182
            +   +E W D+ F S   + D PS  + FGP+ +A ++YQLS  +D+ LAK LVR   L
Sbjct: 127 RKETSREEWLDTVFTS--EKPDFPSEFWIFGPEFMAKNLYQLSPVQDLELAKMLVRANPL 186

Query: 183 FPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQNYGIENVMEIEGADHMAM 242
             +++ +  +F+E+ YGSV ++++VC +D +   + Q+ +I N+  + VMEI+ ADHM M
Sbjct: 187 IKKDMAERRSFSEEGYGSVTRIFIVCGKDLVSPEDYQRSMISNFPPKEVMEIKDADHMPM 246

Query: 243 FSKPLQLFQCLLQVACNY 260
           FSKP QL   LL++A  Y
Sbjct: 247 FSKPQQLCALLLEIANKY 262

BLAST of Spg013515 vs. TAIR 10
Match: AT2G23580.1 (methyl esterase 4 )

HSP 1 Score: 242.7 bits (618), Expect = 3.4e-64
Identity = 119/258 (46.12%), Postives = 174/258 (67.44%), Query Frame = 0

Query: 3   QKHFVLVHGAGHGAWTWYRIKPLLEAASHRVTVLDMVGAGINRKAIQEVKSVVEYAEPLL 62
           +K FVLVHG  HGAW WY++K  LEA  H VT +D+  +GIN   ++E++++ +Y +PLL
Sbjct: 6   KKRFVLVHGLCHGAWCWYKVKTHLEAVGHCVTAVDLAASGINMTRLEEIQTLKDYCKPLL 65

Query: 63  ETMVSVG-PNEKVILVGHSFGGISLAVAMEKFPEKISASVFLTAFAPDTQHKPSYVVDRF 122
           E + S+G  ++KVILV HS GGI  A+A + FP KI+  VFLTAF PDT++ P+YV  + 
Sbjct: 66  ELLNSLGSDDDKVILVAHSMGGIPAALASDIFPSKIATIVFLTAFMPDTRNLPAYVYQKL 125

Query: 123 IESVPKEIWADSQFCSHENRVDGPSTSFSFGPKCLADSVYQLSSPEDVALAKALVRQASL 182
           I SVP+E W D+ F ++  + + P     FGPK +A ++YQLS  +D+ LAK LVR   +
Sbjct: 126 IRSVPQEGWLDTVFGTY-GKHECPLEFALFGPKFMAKNLYQLSPVQDLELAKMLVRVNPI 185

Query: 183 FPEELGKAENFTEKNYGSVKKVYVVCNEDKLITPELQKWIIQNYGIENVMEIEGADHMAM 242
               L    +F+E+ YG+V ++Y+VC ED  +  + Q W+I+N+  + VMEI+ ADHMAM
Sbjct: 186 ITNNLAGTRSFSEEGYGTVTRIYIVCGEDMAVPEDYQWWMIKNFPPKEVMEIKCADHMAM 245

Query: 243 FSKPLQLFQCLLQVACNY 260
           FSKP +L   L+++AC Y
Sbjct: 246 FSKPHKLCALLVEIACKY 262

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038881260.12.0e-10672.90salicylic acid-binding protein 2-like isoform X1 [Benincasa hispida][more]
XP_011657843.26.1e-10070.23salicylic acid-binding protein 2 [Cucumis sativus] >KAE8647492.1 hypothetical pr... [more]
XP_022977269.14.0e-9970.16salicylic acid-binding protein 2-like [Cucurbita maxima][more]
XP_022949785.15.7e-9869.23salicylic acid-binding protein 2-like [Cucurbita moschata][more]
KAG6604201.12.8e-9768.85Salicylic acid-binding protein 2, partial [Cucurbita argyrosperma subsp. sororia... [more]
Match NameE-valueIdentityDescription
Q6RYA01.7e-7653.10Salicylic acid-binding protein 2 OS=Nicotiana tabacum OX=4097 GN=SABP2 PE=1 SV=1[more]
Q8S8S92.0e-6949.02Methylesterase 1 OS=Arabidopsis thaliana OX=3702 GN=MES1 PE=1 SV=1[more]
Q9SE934.4e-6947.45Polyneuridine-aldehyde esterase OS=Rauvolfia serpentina OX=4060 GN=PNAE PE=1 SV=... [more]
O804762.9e-6849.61Methylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=MES2 PE=1 SV=1[more]
Q9LFT65.4e-6750.00Alpha-hydroxynitrile lyase OS=Arabidopsis thaliana OX=3702 GN=HNL PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KG726.6e-10069.85AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G490... [more]
A0A6J1IQY11.9e-9970.16salicylic acid-binding protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC11147763... [more]
A0A6J1GDS12.8e-9869.23salicylic acid-binding protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111453... [more]
A0A6J1HDY03.6e-9064.06salicylic acid-binding protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111463... [more]
A0A6J1KUH64.7e-9063.28salicylic acid-binding protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC11149731... [more]
Match NameE-valueIdentityDescription
AT2G23620.11.4e-7049.02methyl esterase 1 [more]
AT2G23600.12.0e-6949.61acetone-cyanohydrin lyase [more]
AT5G10300.13.8e-6850.00methyl esterase 5 [more]
AT2G23610.13.6e-6646.51methyl esterase 3 [more]
AT2G23580.13.4e-6446.12methyl esterase 4 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000073Alpha/beta hydrolase fold-1PFAMPF12697Abhydrolase_6coord: 6..247
e-value: 7.1E-18
score: 66.0
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 1..260
e-value: 1.2E-72
score: 246.8
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 5..254
NoneNo IPR availablePANTHERPTHR10992METHYLESTERASE FAMILY MEMBERcoord: 3..259
NoneNo IPR availablePANTHERPTHR10992:SF1027METHYLESTERASE 1-RELATEDcoord: 3..259

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg013515.1Spg013515.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009694 jasmonic acid metabolic process
biological_process GO:0009696 salicylic acid metabolic process
molecular_function GO:0080030 methyl indole-3-acetate esterase activity
molecular_function GO:0080032 methyl jasmonate esterase activity
molecular_function GO:0080031 methyl salicylate esterase activity