Spg013498 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg013498 vs. NCBI nr
Match: XP_038880986.1 (L-ascorbate oxidase homolog [Benincasa hispida])

HSP 1 Score: 1064.3 bits (2751), Expect = 3.5e-307
Identity = 519/537 (96.65%), Postives = 524/537 (97.58%), Query Frame = 0

Query: 1   MEKLGLLRLICAALALLSISLVNADNPYRFYTWKVTYGTISPLGVPQQVILINGQFPGPK 60
           MEK GLLRLI  ALALLS+SLVNA+NPYRFYTW VTYGTISPLGVPQQVILINGQFPGPK
Sbjct: 1   MEKQGLLRLIFGALALLSVSLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPK 60

Query: 61  LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYAFQTKD 120
           LEVVTNDNIILNL NKLDEP LLTWNGIKQRKNSWQDGVLGTNC IPPNSNYTY+FQTKD
Sbjct: 61  LEVVTNDNIILNLFNKLDEPLLLTWNGIKQRKNSWQDGVLGTNCPIPPNSNYTYSFQTKD 120

Query: 121 QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPIQDGDFTLLIGDWYKTSHKSLQET 180
           QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPIQDGDFTLLIGDWYKTSHKSLQE+
Sbjct: 121 QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPIQDGDFTLLIGDWYKTSHKSLQES 180

Query: 181 LDAGSSLPSPDGVLINGQSHSTFTGDQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG 240
           LDAG SLP PDGVLINGQ+HSTFTGDQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG
Sbjct: 181 LDAGRSLPLPDGVLINGQTHSTFTGDQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG 240

Query: 241 SHTLQNLYDSLDVHVGQSLSVLVTLDQVPKDYYIVASTRFTKIVLTATAVLHYSNSNSPV 300
           SHTLQNLYDSLDVHVGQSLSVLVTLDQVPKDYYIVASTRFTKIV TATAVLHYSNSNSPV
Sbjct: 241 SHTLQNLYDSLDVHVGQSLSVLVTLDQVPKDYYIVASTRFTKIVHTATAVLHYSNSNSPV 300

Query: 301 SGPVPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSAPLIN 360
           SGPVPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSA LIN
Sbjct: 301 SGPVPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSAALIN 360

Query: 361 GKQRYAINRVSYVNSDTPLKLADHFNIPGVFSVDSLQSLPSDGPAFIATSVLPTALHDFV 420
           GKQRYAINRVSYVNSDTPLKLADHFNIPGVFSVDSLQSLPSDGPAFIATSVLPTALHDFV
Sbjct: 361 GKQRYAINRVSYVNSDTPLKLADHFNIPGVFSVDSLQSLPSDGPAFIATSVLPTALHDFV 420

Query: 421 EIVFQNNENTVQSFHLDGYDFWVVGYGNGQWSQASRNSYNLVDALTRHTAQVYPNSWTTI 480
           EIVFQNNENTVQSFHLDGYDFWVVGYG GQWSQ SRNSYNLVDALTRHTAQVYPNSWTTI
Sbjct: 421 EIVFQNNENTVQSFHLDGYDFWVVGYGYGQWSQDSRNSYNLVDALTRHTAQVYPNSWTTI 480

Query: 481 LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPSNALLCGKAVGRH 538
           LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPSNALLCGKAVGRH
Sbjct: 481 LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPSNALLCGKAVGRH 537

BLAST of Spg013498 vs. NCBI nr
Match: XP_023518323.1 (L-ascorbate oxidase homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1060.8 bits (2742), Expect = 3.8e-306
Identity = 512/537 (95.34%), Postives = 527/537 (98.14%), Query Frame = 0

Query: 1   MEKLGLLRLICAALALLSISLVNADNPYRFYTWKVTYGTISPLGVPQQVILINGQFPGPK 60
           MEK GLL LIC ALALLSISLVNA+NPYRFYTW VTYGTISPLGVPQQVILINGQFPGPK
Sbjct: 1   MEKQGLLPLICGALALLSISLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPK 60

Query: 61  LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYAFQTKD 120
           LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTY+FQTKD
Sbjct: 61  LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYSFQTKD 120

Query: 121 QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPIQDGDFTLLIGDWYKTSHKSLQET 180
           QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYP+QDGDFTLL+GDWYKTSHKSLQE+
Sbjct: 121 QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPVQDGDFTLLVGDWYKTSHKSLQES 180

Query: 181 LDAGSSLPSPDGVLINGQSHSTFTGDQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG 240
           LDAG SLP PDGVLINGQ+HSTFTG+QGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG
Sbjct: 181 LDAGRSLPLPDGVLINGQTHSTFTGNQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG 240

Query: 241 SHTLQNLYDSLDVHVGQSLSVLVTLDQVPKDYYIVASTRFTKIVLTATAVLHYSNSNSPV 300
           SHTLQNLYDSLDVHVGQSLSVLVTLDQ PKDYYIVASTRF++ VLTATAVLHYSNSNSPV
Sbjct: 241 SHTLQNLYDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRFSRTVLTATAVLHYSNSNSPV 300

Query: 301 SGPVPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSAPLIN 360
           SGP+PAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSAPLIN
Sbjct: 301 SGPLPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSAPLIN 360

Query: 361 GKQRYAINRVSYVNSDTPLKLADHFNIPGVFSVDSLQSLPSDGPAFIATSVLPTALHDFV 420
           GKQRYAINRVSYVNSDTPLKLAD+FNIPGVFSVDSLQSLPSDGPAFIATSVLPTALHDFV
Sbjct: 361 GKQRYAINRVSYVNSDTPLKLADYFNIPGVFSVDSLQSLPSDGPAFIATSVLPTALHDFV 420

Query: 421 EIVFQNNENTVQSFHLDGYDFWVVGYGNGQWSQASRNSYNLVDALTRHTAQVYPNSWTTI 480
           EIVFQNNE+T+QSFHLDGYDFWVVGYG GQWSQASRNSYNLVDALTRHTAQVYPNSWT I
Sbjct: 421 EIVFQNNEHTMQSFHLDGYDFWVVGYGYGQWSQASRNSYNLVDALTRHTAQVYPNSWTAI 480

Query: 481 LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPSNALLCGKAVGRH 538
           LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPSNA+LCGKA+GRH
Sbjct: 481 LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPSNAMLCGKAMGRH 537

BLAST of Spg013498 vs. NCBI nr
Match: XP_022963145.1 (L-ascorbate oxidase homolog [Cucurbita moschata])

HSP 1 Score: 1055.0 bits (2727), Expect = 2.1e-304
Identity = 510/537 (94.97%), Postives = 525/537 (97.77%), Query Frame = 0

Query: 1   MEKLGLLRLICAALALLSISLVNADNPYRFYTWKVTYGTISPLGVPQQVILINGQFPGPK 60
           MEK GLLRLIC ALALLSISLVNA+NPYRFYTW VTYGTISPLGVPQQVILINGQFPGPK
Sbjct: 1   MEKQGLLRLICGALALLSISLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPK 60

Query: 61  LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYAFQTKD 120
           LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTY+FQTKD
Sbjct: 61  LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYSFQTKD 120

Query: 121 QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPIQDGDFTLLIGDWYKTSHKSLQET 180
           QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYP+QDGDFTLL+GDWYKTSHKSLQE+
Sbjct: 121 QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPVQDGDFTLLVGDWYKTSHKSLQES 180

Query: 181 LDAGSSLPSPDGVLINGQSHSTFTGDQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG 240
           LDAG SLP PDGVLINGQ+HSTFTG+QGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG
Sbjct: 181 LDAGRSLPLPDGVLINGQTHSTFTGNQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG 240

Query: 241 SHTLQNLYDSLDVHVGQSLSVLVTLDQVPKDYYIVASTRFTKIVLTATAVLHYSNSNSPV 300
           SHTLQNLYDSLDVHVGQSLSVLVTLDQ PKDYYIVASTRF++ VLTATAVLHYSNSNSPV
Sbjct: 241 SHTLQNLYDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRFSRTVLTATAVLHYSNSNSPV 300

Query: 301 SGPVPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSAPLIN 360
           SGP+PAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSAPLIN
Sbjct: 301 SGPLPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSAPLIN 360

Query: 361 GKQRYAINRVSYVNSDTPLKLADHFNIPGVFSVDSLQSLPSDGPAFIATSVLPTALHDFV 420
           GKQRYAINRVSYVNSDTPLKLAD+FNIPGVFSVDSLQSLPS G AFIATSVLPTALHDFV
Sbjct: 361 GKQRYAINRVSYVNSDTPLKLADYFNIPGVFSVDSLQSLPSGGAAFIATSVLPTALHDFV 420

Query: 421 EIVFQNNENTVQSFHLDGYDFWVVGYGNGQWSQASRNSYNLVDALTRHTAQVYPNSWTTI 480
           EIVFQNNE+++QSFHLDGYDFWVVGYG GQWSQASRNSYNLVDALTRHTAQVYPNSWT I
Sbjct: 421 EIVFQNNEHSMQSFHLDGYDFWVVGYGYGQWSQASRNSYNLVDALTRHTAQVYPNSWTAI 480

Query: 481 LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPSNALLCGKAVGRH 538
           LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIP NALLCGKA+GRH
Sbjct: 481 LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPPNALLCGKAMGRH 537

BLAST of Spg013498 vs. NCBI nr
Match: KAG7027071.1 (hypothetical protein SDJN02_11080 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1054.7 bits (2726), Expect = 2.7e-304
Identity = 509/537 (94.79%), Postives = 525/537 (97.77%), Query Frame = 0

Query: 1   MEKLGLLRLICAALALLSISLVNADNPYRFYTWKVTYGTISPLGVPQQVILINGQFPGPK 60
           MEK GLLRLIC ALALLSISLVNA+NPYRFYTW VTYGTISPLGVPQQVILINGQFPGPK
Sbjct: 1   MEKQGLLRLICGALALLSISLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPK 60

Query: 61  LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYAFQTKD 120
           LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTY+FQTKD
Sbjct: 61  LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYSFQTKD 120

Query: 121 QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPIQDGDFTLLIGDWYKTSHKSLQET 180
           QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYP+QDGDFTLL+GDWYKTSHKSLQE+
Sbjct: 121 QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPVQDGDFTLLVGDWYKTSHKSLQES 180

Query: 181 LDAGSSLPSPDGVLINGQSHSTFTGDQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG 240
           LDAG SLP PDGVLINGQ+HSTFTG+QGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG
Sbjct: 181 LDAGRSLPLPDGVLINGQTHSTFTGNQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG 240

Query: 241 SHTLQNLYDSLDVHVGQSLSVLVTLDQVPKDYYIVASTRFTKIVLTATAVLHYSNSNSPV 300
           SHTLQNLYDSLDVHVGQSLSVLVTLDQ PKDYYIVASTRF++ VLTATAVLHYSNSNSPV
Sbjct: 241 SHTLQNLYDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRFSRTVLTATAVLHYSNSNSPV 300

Query: 301 SGPVPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSAPLIN 360
           SGP+PAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKI+PTKTIVLATSAPLIN
Sbjct: 301 SGPLPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIVPTKTIVLATSAPLIN 360

Query: 361 GKQRYAINRVSYVNSDTPLKLADHFNIPGVFSVDSLQSLPSDGPAFIATSVLPTALHDFV 420
           GKQRYAINRVSYVNSDTPLKLAD+FNIPGVFSVDSLQSLPS G AFIATSVLPTALHDFV
Sbjct: 361 GKQRYAINRVSYVNSDTPLKLADYFNIPGVFSVDSLQSLPSGGAAFIATSVLPTALHDFV 420

Query: 421 EIVFQNNENTVQSFHLDGYDFWVVGYGNGQWSQASRNSYNLVDALTRHTAQVYPNSWTTI 480
           EIVFQNNE+++QSFHLDGYDFWVVGYG GQWSQASRNSYNLVDALTRHTAQVYPNSWT I
Sbjct: 421 EIVFQNNEHSMQSFHLDGYDFWVVGYGYGQWSQASRNSYNLVDALTRHTAQVYPNSWTAI 480

Query: 481 LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPSNALLCGKAVGRH 538
           LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIP NALLCGKA+GRH
Sbjct: 481 LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPPNALLCGKAMGRH 537

BLAST of Spg013498 vs. NCBI nr
Match: XP_008440238.1 (PREDICTED: L-ascorbate oxidase homolog [Cucumis melo])

HSP 1 Score: 1052.7 bits (2721), Expect = 1.0e-303
Identity = 511/537 (95.16%), Postives = 521/537 (97.02%), Query Frame = 0

Query: 1   MEKLGLLRLICAALALLSISLVNADNPYRFYTWKVTYGTISPLGVPQQVILINGQFPGPK 60
           MEK GLLRLI  ALALLS+SLVNA+NPYRFYTW VTYGTISPLGVPQQVILINGQFPGPK
Sbjct: 1   MEKQGLLRLIFGALALLSVSLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPK 60

Query: 61  LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYAFQTKD 120
           LEVVTNDNIILNL NKLDEP LLTWNGIKQRKNSWQDGV+GTNC IPPNSNYTY+FQTKD
Sbjct: 61  LEVVTNDNIILNLFNKLDEPLLLTWNGIKQRKNSWQDGVVGTNCPIPPNSNYTYSFQTKD 120

Query: 121 QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPIQDGDFTLLIGDWYKTSHKSLQET 180
           QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPIQDGDFTLLIGDWYKTSHKSLQE+
Sbjct: 121 QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPIQDGDFTLLIGDWYKTSHKSLQES 180

Query: 181 LDAGSSLPSPDGVLINGQSHSTFTGDQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG 240
           LD+G SLP PDGVLINGQ+HSTFTGDQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG
Sbjct: 181 LDSGGSLPLPDGVLINGQTHSTFTGDQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG 240

Query: 241 SHTLQNLYDSLDVHVGQSLSVLVTLDQVPKDYYIVASTRFTKIVLTATAVLHYSNSNSPV 300
           SHTLQNLYDSLDVHVGQSLSVLVTLDQ PKDYYIVASTRFTK VLTATAVLHYSNSNSPV
Sbjct: 241 SHTLQNLYDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRFTKTVLTATAVLHYSNSNSPV 300

Query: 301 SGPVPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSAPLIN 360
           SGP+PAGPT EVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSA LIN
Sbjct: 301 SGPLPAGPTTEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSAALIN 360

Query: 361 GKQRYAINRVSYVNSDTPLKLADHFNIPGVFSVDSLQSLPSDGPAFIATSVLPTALHDFV 420
           GKQRYAINRVSYVNSDTPLKLAD+FNIPGVFSVDSLQSLPSDGPAFIATSVLPTALHDFV
Sbjct: 361 GKQRYAINRVSYVNSDTPLKLADYFNIPGVFSVDSLQSLPSDGPAFIATSVLPTALHDFV 420

Query: 421 EIVFQNNENTVQSFHLDGYDFWVVGYGNGQWSQASRNSYNLVDALTRHTAQVYPNSWTTI 480
           EIVFQNNENTVQSFHLDGYDFWVVGYG GQWSQ SRNSYNLVDALTRHTAQVYPNSWTTI
Sbjct: 421 EIVFQNNENTVQSFHLDGYDFWVVGYGYGQWSQDSRNSYNLVDALTRHTAQVYPNSWTTI 480

Query: 481 LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPSNALLCGKAVGRH 538
           LVSLDNQGMWNLRS+IWERQYLGQQFYLRVWT TQSLANEYNIPSNALLCGKAVGRH
Sbjct: 481 LVSLDNQGMWNLRSSIWERQYLGQQFYLRVWTPTQSLANEYNIPSNALLCGKAVGRH 537

BLAST of Spg013498 vs. ExPASy Swiss-Prot
Match: P29162 (L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 599.0 bits (1543), Expect = 5.4e-170
Identity = 280/537 (52.14%), Postives = 377/537 (70.20%), Query Frame = 0

Query: 12  AALALLSISLVNADNPYRFYTWKVTYGTISPLGVPQQVILINGQFPGPKLEVVTNDNIIL 71
           A L  LS+ ++ A++PY ++ W VTYGTI+PLGVPQQ ILINGQFPGP++   +N+NI++
Sbjct: 10  ALLLCLSVGVI-AEDPYLYFNWNVTYGTIAPLGVPQQGILINGQFPGPRINCTSNNNIVV 69

Query: 72  NLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYAFQTKDQIGSFAYFPST 131
           N+ N LDEPFL TWNG++ RKNSWQDG  GT C I P  N+TY FQ KDQIGS++YFP+T
Sbjct: 70  NVFNNLDEPFLFTWNGVQHRKNSWQDGTPGTMCPIMPGQNFTYRFQVKDQIGSYSYFPTT 129

Query: 132 QFHKAAGGFGALNVYQRPRIPIPYPIQDGDFTLLIGDWYKTSHKSLQETLDAGSSLPSPD 191
             H+AAGG+GALNV+ R  IP+P+     ++ + +GDWY   HK+L++ LD G ++  PD
Sbjct: 130 ALHRAAGGYGALNVHSRALIPVPFDNPADEYNVFVGDWYNKGHKTLKKILDGGRTIGRPD 189

Query: 192 GVLINGQS-------HSTFTGDQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEGSHTL 251
           G++ING+S          FT + GKTY +R  N+G+ +S+N R QGH MKLVE+EGSHT+
Sbjct: 190 GIIINGKSAKVGEAKEPLFTMEAGKTYRYRFCNLGMRSSVNIRFQGHPMKLVELEGSHTV 249

Query: 252 QNLYDSLDVHVGQSLSVLVTLDQVPKDYYIVASTRFTKIVLTATAVLHYSNSNSPVSGPV 311
           QN+YDSLD+HVGQ LSVLVT DQ PKDYY+V S+RF K  L++ A++ Y+N   P S  +
Sbjct: 250 QNIYDSLDLHVGQCLSVLVTADQEPKDYYLVVSSRFLKQALSSVAIIRYANGKGPASPEL 309

Query: 312 PAGP---TIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSAPLING 371
           P  P   T  + WS+ Q R+FRWNLTA+AARPNPQGS+HYG+I  T+TI +  S   + G
Sbjct: 310 PTPPPENTEGIAWSMNQFRSFRWNLTASAARPNPQGSYHYGQINITRTIKIFNSMSQVGG 369

Query: 372 KQRYAINRVSYVNSDTPLKLADHFNIPG-VFSVDSL-QSLPSD-GPAFIATSVLPTALHD 431
           K RY +N +S+ N +TPLKL ++F      F  D +    P+D     IAT+V      +
Sbjct: 370 KLRYGLNGISHTNGETPLKLVEYFGATNKAFKYDLMADEAPADPSKLTIATNVKNATYRN 429

Query: 432 FVEIVFQNNENTVQSFHLDGYDFWVVGYGNGQWSQASRNSYNLVDALTRHTAQVYPNSWT 491
           FVEI+F+N+E T++++HLDGY F+ V    G+WS   R +YNLVD L+R+  QVYPNSW 
Sbjct: 430 FVEIIFENHEKTIRTYHLDGYSFFAVAVEPGRWSPEKRKNYNLVDGLSRNNIQVYPNSWA 489

Query: 492 TILVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPSNALLCGKAVG 536
            I+++ DN GMWNLRS +WE+ YLG+Q Y  V + ++SL +EYNIP N  LCG   G
Sbjct: 490 AIMLTFDNAGMWNLRSEMWEKTYLGEQLYFSVLSPSRSLRDEYNIPDNHPLCGIVKG 545

BLAST of Spg013498 vs. ExPASy Swiss-Prot
Match: Q00624 (L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1)

HSP 1 Score: 590.9 bits (1522), Expect = 1.5e-167
Identity = 289/532 (54.32%), Postives = 368/532 (69.17%), Query Frame = 0

Query: 11  CAALALLSISLVNADNPYRFYTWKVTYGTISPLGVPQQVILINGQFPGPKLEVVTNDNII 70
           C  LA  +  +V+A++PY  + W VTYGT SPLGVPQQVILINGQFPGP +   +N+N+I
Sbjct: 10  CLYLAAAATVVVHAEDPYFHHVWNVTYGTASPLGVPQQVILINGQFPGPNINSTSNNNVI 69

Query: 71  LNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYAFQTKDQIGSFAYFPS 130
           +N+ N LDEPFLLTWNGI+ RKN WQDG  GT C I P +NYTY FQ KDQIGS+ Y+P+
Sbjct: 70  INVFNNLDEPFLLTWNGIQHRKNCWQDGTPGTMCPIMPGTNYTYHFQPKDQIGSYFYYPT 129

Query: 131 TQFHKAAGGFGALNVYQRPRIPIPYPIQDGDFTLLIGDWYKTSHKSLQETLDAGSSLPSP 190
           T  H+AAGG+G L V  R  IP+PY   + D+T+LIGDWY  SH  L++ LD G ++  P
Sbjct: 130 TGMHRAAGGYGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTQLKKFLDGGRTIGRP 189

Query: 191 DGVLINGQSHS-------TFTGDQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEGSHT 250
           DG++ING+S          FT   GKTY  RI NVG+ TS+NFRIQ HKMKLVE+EGSH 
Sbjct: 190 DGIVINGKSGKGDGSDAPLFTLKPGKTYRVRICNVGVKTSINFRIQNHKMKLVEMEGSHV 249

Query: 251 LQNLYDSLDVHVGQSLSVLVTLDQVPKDYYIVASTRFTKIVLTATAVLHYSNSNSPVSGP 310
           LQN YDSLDVHVGQ    +VT +Q PKDYY+VAS+RF K V+T T +L Y     P S  
Sbjct: 250 LQNDYDSLDVHVGQCFGTIVTANQEPKDYYMVASSRFLKTVITTTGLLRYEGGKGPASSQ 309

Query: 311 VPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSAPLINGKQ 370
           +PAGP +   WS+ Q R+FRWNLTA+AARPNPQGS+HYGKI  T+TI L  +   ++GK 
Sbjct: 310 LPAGP-VGWAWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNTQGKVDGKL 369

Query: 371 RYAINRVSYVNSDTPLKLADHFNIPG-VFSVDSLQSLPSDGP---AFIATSVLPTALHDF 430
           R+A+N VS+   +TPLKLA++F I   VF  D++   P+        I  +VL      F
Sbjct: 370 RFALNGVSHTEPETPLKLAEYFGISDKVFKYDTITDDPTPEQIKNIKIEPNVLNITHRTF 429

Query: 431 VEIVFQNNENTVQSFHLDGYDFWVVGYGNGQWSQASRNSYNLVDALTRHTAQVYPNSWTT 490
           VE+VF+N+E +VQS+HLDGY F+ V    G W+   R +YNL+DA++RHT QVYP  W  
Sbjct: 430 VEVVFENHEKSVQSWHLDGYSFFSVAVEPGTWTPEKRKNYNLLDAVSRHTVQVYPKCWAA 489

Query: 491 ILVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPSNALLCG 532
           IL++ DN GMWN+RS   ER+YLGQQ Y  V +  +SL +EYN+P  +L CG
Sbjct: 490 ILLTFDNCGMWNVRSENTERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCG 540

BLAST of Spg013498 vs. ExPASy Swiss-Prot
Match: Q9SU40 (Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=1 SV=1)

HSP 1 Score: 537.0 bits (1382), Expect = 2.5e-151
Identity = 270/537 (50.28%), Postives = 350/537 (65.18%), Query Frame = 0

Query: 17  LSISLVNADNPYRFYTWKVTYGTISPLGVPQQVILINGQFPGPKLEVVTNDNIILNLINK 76
           ++IS   A +PY FY ++V+Y T SPLGVPQQVI ING+FPGP + V TN+N+++N+ NK
Sbjct: 13  VNISFCFAADPYSFYNFEVSYITASPLGVPQQVIAINGKFPGPTINVTTNENLVVNVRNK 72

Query: 77  LDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYAFQTKDQIGSFAYFPSTQFHKA 136
           LDE  LL WNGI+QR+ SWQDGVLGTNC IPP  N+TY FQ KDQIGSF YFPS  F +A
Sbjct: 73  LDEGLLLHWNGIQQRRVSWQDGVLGTNCPIPPKWNWTYEFQVKDQIGSFFYFPSLHFQRA 132

Query: 137 AGGFGALNVYQRPRIPIPYPIQDGDFTLLIGDWYKTSHKSLQETLDAGSSLPSPDGVLIN 196
           +GGFG+  V  R  IP+P+   DGD T+ IGDWY  +H +L++ LD G  L  PDGVLIN
Sbjct: 133 SGGFGSFVVNPRAIIPVPFSTPDGDITVTIGDWYIRNHTALRKALDDGKDLGMPDGVLIN 192

Query: 197 GQ--------------SHSTFTGDQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEGSH 256
           G+                 T T   GKTY  R+SNVG+STSLNFRIQGH + L E EGS+
Sbjct: 193 GKGPYRYNDTLVADGIDFETITVHPGKTYRLRVSNVGISTSLNFRIQGHNLVLAESEGSY 252

Query: 257 TLQNLYDSLDVHVGQSLSVLVTLDQ-VPKDYYIVASTRFTKIVL----TATAVLHYSNSN 316
           T+Q  Y SLD+HVGQS S LVT+DQ    DYYIVAS R     +    T   +L Y+NS 
Sbjct: 253 TVQQNYTSLDIHVGQSYSFLVTMDQNASSDYYIVASARVVNETIWRRVTGVGILKYTNSK 312

Query: 317 SPVSGPVPAGPTIEVD--WSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATS 376
               G +P GP  E D  +S+ QAR+ RWN++A+ ARPNPQGSF YG I  T   VL   
Sbjct: 313 GKAKGQLPPGPQDEFDKTFSMNQARSIRWNVSASGARPNPQGSFKYGSINVTDVYVLRNM 372

Query: 377 APL-INGKQRYAINRVSYVNSDTPLKLADHFNIPGVFSVDSLQSLPSDGPAFIATSVLPT 436
            P+ I+GK+R  +N +S+ N  TP++LAD   +  V+ +D     P  GPA +ATS++  
Sbjct: 373 PPVTISGKRRTTLNGISFKNPSTPIRLADKLKVKDVYKLD-FPKRPLTGPAKVATSIING 432

Query: 437 ALHDFVEIVFQNNENTVQSFHLDGYDFWVVGYGNGQWSQASRNSYNLVDALTRHTAQVYP 496
               F+E+V QNN+  +QS+H+ GY F+VVG   G+W++ SR +YN  D + R T QVYP
Sbjct: 433 TYRGFMEVVLQNNDTKMQSYHMSGYAFFVVGMDYGEWTENSRGTYNKWDGIARSTIQVYP 492

Query: 497 NSWTTILVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPSNALLCG 532
            +W+ IL+SLDN G WNLR+   +  YLGQ+ Y+RV    ++   E+  P N L CG
Sbjct: 493 GAWSAILISLDNPGAWNLRTENLDSWYLGQETYVRVVNPDENNKTEFGHPDNVLYCG 548

BLAST of Spg013498 vs. ExPASy Swiss-Prot
Match: Q8VXX5 (Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=2 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 1.5e-148
Identity = 269/544 (49.45%), Postives = 351/544 (64.52%), Query Frame = 0

Query: 9   LICAALALLSISLVNADNPYRFYTWKVTYGTISPLGVPQQVILINGQFPGPKLEVVTNDN 68
           L+C AL L ++S   A +P+  Y ++V+Y T SPLGVPQQVI +NGQFPGP L   TN N
Sbjct: 12  LLCFAL-LSAVSF--AADPFVSYDFRVSYLTASPLGVPQQVIAVNGQFPGPLLNATTNYN 71

Query: 69  IILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYAFQTKDQIGSFAYF 128
           +++N+ N LDEP LLTW GI+ R+NSWQDGVLGTNC IPP  N+TY FQ KDQIGSF Y 
Sbjct: 72  VVVNVFNHLDEPLLLTWPGIQMRRNSWQDGVLGTNCPIPPRWNFTYQFQVKDQIGSFFYS 131

Query: 129 PSTQFHKAAGGFGALNVYQRPRIPIPYPIQDGDFTLLIGDWYKTSHKSLQETLDAGSSLP 188
           PS  F +A+GGFG + +  R  IPIP+P  DG+   +IGDWY   HK+L+  LD+G  L 
Sbjct: 132 PSLNFQRASGGFGPIVINNRDIIPIPFPQPDGELIFIIGDWYTQDHKALRRALDSGKELG 191

Query: 189 SPDGVLINGQ-------------SHSTFTGDQGKTYMFRISNVGLSTSLNFRIQGHKMKL 248
            PDGVLING+              + TF  + GKTY  R+ NVG+STSLNFRIQ H + L
Sbjct: 192 MPDGVLINGKGPYKYNSSVPDGIDYLTFHVEPGKTYRIRVHNVGISTSLNFRIQNHSLLL 251

Query: 249 VEVEGSHTLQNLYDSLDVHVGQSLSVLVTLDQ-VPKDYYIVASTRFTKIV----LTATAV 308
           VE EG +T Q  +   DVHVGQS S LVT+DQ    DYYIVAS RF        +T  A+
Sbjct: 252 VETEGHYTSQANFTDFDVHVGQSYSFLVTMDQDATSDYYIVASARFVNETVWQRVTGVAI 311

Query: 309 LHYSNSNSPVSGPVPAGPT-IEVDWS-VKQARTFRWNLTANAARPNPQGSFHYGKIIPTK 368
           LHYSNS  PVSGP+P   T +   WS + Q +T R N +A+ ARPNPQGSFHYG+I  T 
Sbjct: 312 LHYSNSKGPVSGPLPVPKTDVSSPWSAMSQPKTIRQNTSASGARPNPQGSFHYGQINITN 371

Query: 369 TIVLATSAP-LINGKQRYAINRVSYVNSDTPLKLADHFNIPGVFSVDSLQSLPSDGPAFI 428
           T +L +  P +ING  R  +N +S+VN  TP++LAD   + G + +D     P + P  +
Sbjct: 372 TYILRSLPPTIINGALRATLNGISFVNPSTPVRLADRNKVKGAYKLD-FPDRPFNRPLRL 431

Query: 429 ATSVLPTALHDFVEIVFQNNENTVQSFHLDGYDFWVVGYGNGQWSQASRNSYNLVDALTR 488
             S++      F+++VFQNN+  +QSFH+DGY F+VVG   G WS+  + SYN  DA++R
Sbjct: 432 DRSMINATYKGFIQVVFQNNDTKIQSFHVDGYSFFVVGMDFGIWSEDKKGSYNNWDAISR 491

Query: 489 HTAQVYPNSWTTILVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPSNA 532
            T +VYP  WT +L+SLDN G+WN+R    +R YLG++ Y+R+    +    E + P N 
Sbjct: 492 STIEVYPGGWTAVLISLDNVGVWNIRVENLDRWYLGEETYMRITNPEEDGKTEMDPPDNV 551

BLAST of Spg013498 vs. ExPASy Swiss-Prot
Match: Q9FHN6 (Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=2 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 2.1e-145
Identity = 254/529 (48.02%), Postives = 341/529 (64.46%), Query Frame = 0

Query: 24  ADNPYRFYTWKVTYGTISPLGVPQQVILINGQFPGPKLEVVTNDNIILNLINKLDEPFLL 83
           A +PY  Y + ++Y T SPLGVPQQVI +NG+FPGP +   TN N+ +N++N LDEP LL
Sbjct: 23  AGDPYVSYDFTLSYITASPLGVPQQVIAVNGKFPGPVINATTNYNVHVNVLNHLDEPLLL 82

Query: 84  TWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYAFQTKDQIGSFAYFPSTQFHKAAGGFGAL 143
           TW G++ R+NSWQDGVLGTNC IPPN N+TY FQ KDQIGS+ Y PS  F +A+GGFGAL
Sbjct: 83  TWPGVQMRRNSWQDGVLGTNCPIPPNWNFTYDFQLKDQIGSYFYSPSLNFQRASGGFGAL 142

Query: 144 NVYQRPRIPIPYPIQDGDFTLLIGDWYKTSHKSLQETLDAGSSLPSPDGVLINGQ----- 203
            +  R  +PIP+   DG+   +IGDWY  +H +L+  LD+G  L  PDGVLING+     
Sbjct: 143 IINNRDLVPIPFTEPDGEIIFIIGDWYTQNHTALRRILDSGKELGMPDGVLINGKGPFKY 202

Query: 204 --------SHSTFTGDQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEGSHTLQNLYDS 263
                    H T   D GKTY  R+ NVG+STSLNFRIQ HK+ L+E EG +T Q  +  
Sbjct: 203 NSSVPDGIEHETVNVDPGKTYRIRVHNVGISTSLNFRIQNHKLLLIETEGRYTSQMNFTD 262

Query: 264 LDVHVGQSLSVLVTLDQ-VPKDYYIVASTRFTKIV----LTATAVLHYSNSNSPVSGPVP 323
            DVHVGQS S LVT+DQ    DYYIVAS RF        +T   +LHYSNS  P SGP+P
Sbjct: 263 FDVHVGQSYSFLVTMDQNATSDYYIVASARFVNETVWQRVTGVGILHYSNSKGPASGPLP 322

Query: 324 AGPT-IEVDWS-VKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSAPL-INGK 383
              T +   WS + Q R  + N +A+ ARPNPQGSFHYG+I  T+T +L +  P  INGK
Sbjct: 323 VSATDVNHPWSAMNQPRAIKQNTSASGARPNPQGSFHYGQINITRTYILRSLPPTKINGK 382

Query: 384 QRYAINRVSYVNSDTPLKLADHFNIPGVFSVDSLQSLPSDGPAFIATSVLPTALHDFVEI 443
            R  +N +S+VN  TP++LAD   + G + +D       +    +++S++      F+++
Sbjct: 383 LRATLNGISFVNPSTPMRLADDHKVKGDYMLDFPDRPLDEKLPRLSSSIINATYKGFIQV 442

Query: 444 VFQNNENTVQSFHLDGYDFWVVGYGNGQWSQASRNSYNLVDALTRHTAQVYPNSWTTILV 503
           +FQNN+  +QSFH+DGY F+VV    G WS+   +SYN  DA+ R T +VYP +WT +L+
Sbjct: 443 IFQNNDTKIQSFHIDGYAFYVVAMDFGIWSEDRNSSYNNWDAVARSTVEVYPGAWTAVLI 502

Query: 504 SLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPSNALLCG 532
           SLDN G+WN+R    +R YLGQ+ Y+R+    ++ + E + P N + CG
Sbjct: 503 SLDNVGVWNIRVENLDRWYLGQETYMRIINPEENGSTEMDPPENVMYCG 551

BLAST of Spg013498 vs. ExPASy TrEMBL
Match: A0A6J1HH59 (L-ascorbate oxidase homolog OS=Cucurbita moschata OX=3662 GN=LOC111463442 PE=3 SV=1)

HSP 1 Score: 1055.0 bits (2727), Expect = 1.0e-304
Identity = 510/537 (94.97%), Postives = 525/537 (97.77%), Query Frame = 0

Query: 1   MEKLGLLRLICAALALLSISLVNADNPYRFYTWKVTYGTISPLGVPQQVILINGQFPGPK 60
           MEK GLLRLIC ALALLSISLVNA+NPYRFYTW VTYGTISPLGVPQQVILINGQFPGPK
Sbjct: 1   MEKQGLLRLICGALALLSISLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPK 60

Query: 61  LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYAFQTKD 120
           LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTY+FQTKD
Sbjct: 61  LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYSFQTKD 120

Query: 121 QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPIQDGDFTLLIGDWYKTSHKSLQET 180
           QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYP+QDGDFTLL+GDWYKTSHKSLQE+
Sbjct: 121 QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPVQDGDFTLLVGDWYKTSHKSLQES 180

Query: 181 LDAGSSLPSPDGVLINGQSHSTFTGDQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG 240
           LDAG SLP PDGVLINGQ+HSTFTG+QGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG
Sbjct: 181 LDAGRSLPLPDGVLINGQTHSTFTGNQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG 240

Query: 241 SHTLQNLYDSLDVHVGQSLSVLVTLDQVPKDYYIVASTRFTKIVLTATAVLHYSNSNSPV 300
           SHTLQNLYDSLDVHVGQSLSVLVTLDQ PKDYYIVASTRF++ VLTATAVLHYSNSNSPV
Sbjct: 241 SHTLQNLYDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRFSRTVLTATAVLHYSNSNSPV 300

Query: 301 SGPVPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSAPLIN 360
           SGP+PAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSAPLIN
Sbjct: 301 SGPLPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSAPLIN 360

Query: 361 GKQRYAINRVSYVNSDTPLKLADHFNIPGVFSVDSLQSLPSDGPAFIATSVLPTALHDFV 420
           GKQRYAINRVSYVNSDTPLKLAD+FNIPGVFSVDSLQSLPS G AFIATSVLPTALHDFV
Sbjct: 361 GKQRYAINRVSYVNSDTPLKLADYFNIPGVFSVDSLQSLPSGGAAFIATSVLPTALHDFV 420

Query: 421 EIVFQNNENTVQSFHLDGYDFWVVGYGNGQWSQASRNSYNLVDALTRHTAQVYPNSWTTI 480
           EIVFQNNE+++QSFHLDGYDFWVVGYG GQWSQASRNSYNLVDALTRHTAQVYPNSWT I
Sbjct: 421 EIVFQNNEHSMQSFHLDGYDFWVVGYGYGQWSQASRNSYNLVDALTRHTAQVYPNSWTAI 480

Query: 481 LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPSNALLCGKAVGRH 538
           LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIP NALLCGKA+GRH
Sbjct: 481 LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPPNALLCGKAMGRH 537

BLAST of Spg013498 vs. ExPASy TrEMBL
Match: A0A1S3B0P0 (L-ascorbate oxidase homolog OS=Cucumis melo OX=3656 GN=LOC103484753 PE=3 SV=1)

HSP 1 Score: 1052.7 bits (2721), Expect = 5.0e-304
Identity = 511/537 (95.16%), Postives = 521/537 (97.02%), Query Frame = 0

Query: 1   MEKLGLLRLICAALALLSISLVNADNPYRFYTWKVTYGTISPLGVPQQVILINGQFPGPK 60
           MEK GLLRLI  ALALLS+SLVNA+NPYRFYTW VTYGTISPLGVPQQVILINGQFPGPK
Sbjct: 1   MEKQGLLRLIFGALALLSVSLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPK 60

Query: 61  LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYAFQTKD 120
           LEVVTNDNIILNL NKLDEP LLTWNGIKQRKNSWQDGV+GTNC IPPNSNYTY+FQTKD
Sbjct: 61  LEVVTNDNIILNLFNKLDEPLLLTWNGIKQRKNSWQDGVVGTNCPIPPNSNYTYSFQTKD 120

Query: 121 QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPIQDGDFTLLIGDWYKTSHKSLQET 180
           QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPIQDGDFTLLIGDWYKTSHKSLQE+
Sbjct: 121 QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPIQDGDFTLLIGDWYKTSHKSLQES 180

Query: 181 LDAGSSLPSPDGVLINGQSHSTFTGDQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG 240
           LD+G SLP PDGVLINGQ+HSTFTGDQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG
Sbjct: 181 LDSGGSLPLPDGVLINGQTHSTFTGDQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG 240

Query: 241 SHTLQNLYDSLDVHVGQSLSVLVTLDQVPKDYYIVASTRFTKIVLTATAVLHYSNSNSPV 300
           SHTLQNLYDSLDVHVGQSLSVLVTLDQ PKDYYIVASTRFTK VLTATAVLHYSNSNSPV
Sbjct: 241 SHTLQNLYDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRFTKTVLTATAVLHYSNSNSPV 300

Query: 301 SGPVPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSAPLIN 360
           SGP+PAGPT EVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSA LIN
Sbjct: 301 SGPLPAGPTTEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSAALIN 360

Query: 361 GKQRYAINRVSYVNSDTPLKLADHFNIPGVFSVDSLQSLPSDGPAFIATSVLPTALHDFV 420
           GKQRYAINRVSYVNSDTPLKLAD+FNIPGVFSVDSLQSLPSDGPAFIATSVLPTALHDFV
Sbjct: 361 GKQRYAINRVSYVNSDTPLKLADYFNIPGVFSVDSLQSLPSDGPAFIATSVLPTALHDFV 420

Query: 421 EIVFQNNENTVQSFHLDGYDFWVVGYGNGQWSQASRNSYNLVDALTRHTAQVYPNSWTTI 480
           EIVFQNNENTVQSFHLDGYDFWVVGYG GQWSQ SRNSYNLVDALTRHTAQVYPNSWTTI
Sbjct: 421 EIVFQNNENTVQSFHLDGYDFWVVGYGYGQWSQDSRNSYNLVDALTRHTAQVYPNSWTTI 480

Query: 481 LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPSNALLCGKAVGRH 538
           LVSLDNQGMWNLRS+IWERQYLGQQFYLRVWT TQSLANEYNIPSNALLCGKAVGRH
Sbjct: 481 LVSLDNQGMWNLRSSIWERQYLGQQFYLRVWTPTQSLANEYNIPSNALLCGKAVGRH 537

BLAST of Spg013498 vs. ExPASy TrEMBL
Match: A0A5D3CN89 (L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G005040 PE=3 SV=1)

HSP 1 Score: 1052.4 bits (2720), Expect = 6.6e-304
Identity = 510/537 (94.97%), Postives = 521/537 (97.02%), Query Frame = 0

Query: 1   MEKLGLLRLICAALALLSISLVNADNPYRFYTWKVTYGTISPLGVPQQVILINGQFPGPK 60
           MEK GLLRLI  ALALLS+SLVNA+NPYRFYTW VTYGTISPLGVPQQVILINGQFPGPK
Sbjct: 1   MEKQGLLRLIFGALALLSVSLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPK 60

Query: 61  LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYAFQTKD 120
           LEVVTNDNIILNL NKLDEP LLTWNGIKQRKNSWQDGV+GTNC IPPNSNYTY+FQTKD
Sbjct: 61  LEVVTNDNIILNLFNKLDEPLLLTWNGIKQRKNSWQDGVVGTNCPIPPNSNYTYSFQTKD 120

Query: 121 QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPIQDGDFTLLIGDWYKTSHKSLQET 180
           QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPIQDGDFTLLIGDWYKTSHKSLQE+
Sbjct: 121 QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPIQDGDFTLLIGDWYKTSHKSLQES 180

Query: 181 LDAGSSLPSPDGVLINGQSHSTFTGDQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG 240
           LD+G SLP PDGVL+NGQ+HSTFTGDQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG
Sbjct: 181 LDSGGSLPLPDGVLVNGQTHSTFTGDQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG 240

Query: 241 SHTLQNLYDSLDVHVGQSLSVLVTLDQVPKDYYIVASTRFTKIVLTATAVLHYSNSNSPV 300
           SHTLQNLYDSLDVHVGQSLSVLVTLDQ PKDYYIVASTRFTK VLTATAVLHYSNSNSPV
Sbjct: 241 SHTLQNLYDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRFTKTVLTATAVLHYSNSNSPV 300

Query: 301 SGPVPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSAPLIN 360
           SGP+PAGPT EVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSA LIN
Sbjct: 301 SGPLPAGPTTEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSAALIN 360

Query: 361 GKQRYAINRVSYVNSDTPLKLADHFNIPGVFSVDSLQSLPSDGPAFIATSVLPTALHDFV 420
           GKQRYAINRVSYVNSDTPLKLAD+FNIPGVFSVDSLQSLPSDGPAFIATSVLPTALHDFV
Sbjct: 361 GKQRYAINRVSYVNSDTPLKLADYFNIPGVFSVDSLQSLPSDGPAFIATSVLPTALHDFV 420

Query: 421 EIVFQNNENTVQSFHLDGYDFWVVGYGNGQWSQASRNSYNLVDALTRHTAQVYPNSWTTI 480
           EIVFQNNENTVQSFHLDGYDFWVVGYG GQWSQ SRNSYNLVDALTRHTAQVYPNSWTTI
Sbjct: 421 EIVFQNNENTVQSFHLDGYDFWVVGYGYGQWSQDSRNSYNLVDALTRHTAQVYPNSWTTI 480

Query: 481 LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPSNALLCGKAVGRH 538
           LVSLDNQGMWNLRS+IWERQYLGQQFYLRVWT TQSLANEYNIPSNALLCGKAVGRH
Sbjct: 481 LVSLDNQGMWNLRSSIWERQYLGQQFYLRVWTPTQSLANEYNIPSNALLCGKAVGRH 537

BLAST of Spg013498 vs. ExPASy TrEMBL
Match: A0A6J1BV74 (L-ascorbate oxidase homolog OS=Momordica charantia OX=3673 GN=LOC111005834 PE=3 SV=1)

HSP 1 Score: 1050.8 bits (2716), Expect = 1.9e-303
Identity = 509/536 (94.96%), Postives = 524/536 (97.76%), Query Frame = 0

Query: 1   MEKLGLLRLICAALALLSISLVNADNPYRFYTWKVTYGTISPLGVPQQVILINGQFPGPK 60
           MEK G L LI AALA L++SLVNAD+PYRFYTW VTYGTISPLGVPQQVILINGQFPGPK
Sbjct: 1   MEKQGRLPLIFAALAFLTLSLVNADDPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPK 60

Query: 61  LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYAFQTKD 120
           LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNC IPPNSNYTYAFQTKD
Sbjct: 61  LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCPIPPNSNYTYAFQTKD 120

Query: 121 QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPIQDGDFTLLIGDWYKTSHKSLQET 180
           QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPIQDGDFTLLIGDWYK SHKSLQE+
Sbjct: 121 QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPIQDGDFTLLIGDWYKNSHKSLQES 180

Query: 181 LDAGSSLPSPDGVLINGQSHSTFTGDQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG 240
           LDAG SLP PDGVLINGQ+HSTF+GDQGKTYMFRISNVGLSTSLNFRIQGHK+KLVEVEG
Sbjct: 181 LDAGRSLPLPDGVLINGQTHSTFSGDQGKTYMFRISNVGLSTSLNFRIQGHKLKLVEVEG 240

Query: 241 SHTLQNLYDSLDVHVGQSLSVLVTLDQVPKDYYIVASTRFTKIVLTATAVLHYSNSNSPV 300
           SHTLQNLYDSLDVHVGQSLSVLVTLDQVPKDYYIVASTRFTKIVLTATAVLHYSNS+SPV
Sbjct: 241 SHTLQNLYDSLDVHVGQSLSVLVTLDQVPKDYYIVASTRFTKIVLTATAVLHYSNSHSPV 300

Query: 301 SGPVPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSAPLIN 360
           SGP+PAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSAPLIN
Sbjct: 301 SGPLPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSAPLIN 360

Query: 361 GKQRYAINRVSYVNSDTPLKLADHFNIPGVFSVDSLQSLPSDGPAFIATSVLPTALHDFV 420
           GKQRYAINRVS+VNSDTPLKLADHFNIPGVFSVDSLQSLPSDGPAF+ATSVLPT+LHDFV
Sbjct: 361 GKQRYAINRVSFVNSDTPLKLADHFNIPGVFSVDSLQSLPSDGPAFVATSVLPTSLHDFV 420

Query: 421 EIVFQNNENTVQSFHLDGYDFWVVGYGNGQWSQASRNSYNLVDALTRHTAQVYPNSWTTI 480
           EIVFQNNENT+QSFHLDGYDFWVVGYG GQW+QASRNSYNLVDALTRHTAQVYPNSWTTI
Sbjct: 421 EIVFQNNENTMQSFHLDGYDFWVVGYGYGQWTQASRNSYNLVDALTRHTAQVYPNSWTTI 480

Query: 481 LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPSNALLCGKAVGR 537
           LVSLDNQGMWNLRSA+WERQYLGQQFYLRVWTQTQSLANEY IPSNALLCGKAVGR
Sbjct: 481 LVSLDNQGMWNLRSAMWERQYLGQQFYLRVWTQTQSLANEYGIPSNALLCGKAVGR 536

BLAST of Spg013498 vs. ExPASy TrEMBL
Match: A0A6J1KP89 (L-ascorbate oxidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111497047 PE=3 SV=1)

HSP 1 Score: 1049.7 bits (2713), Expect = 4.3e-303
Identity = 507/537 (94.41%), Postives = 524/537 (97.58%), Query Frame = 0

Query: 1   MEKLGLLRLICAALALLSISLVNADNPYRFYTWKVTYGTISPLGVPQQVILINGQFPGPK 60
           MEK GLLRLIC ALALLSISLVNA+NPYRFYTW VTYGTISPLGVPQQVILINGQFPGPK
Sbjct: 1   MEKQGLLRLICRALALLSISLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPK 60

Query: 61  LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYAFQTKD 120
           LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTY+FQTKD
Sbjct: 61  LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYSFQTKD 120

Query: 121 QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPIQDGDFTLLIGDWYKTSHKSLQET 180
           QIGSFAYFPSTQF KA GGFGALNVYQRPRIPIPYP+QDGDFTLL+GDWYKTSHKSLQE+
Sbjct: 121 QIGSFAYFPSTQFQKAGGGFGALNVYQRPRIPIPYPVQDGDFTLLVGDWYKTSHKSLQES 180

Query: 181 LDAGSSLPSPDGVLINGQSHSTFTGDQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG 240
           LDAG SLP PDGVLINGQ+HSTFTG+QGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG
Sbjct: 181 LDAGRSLPLPDGVLINGQTHSTFTGNQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG 240

Query: 241 SHTLQNLYDSLDVHVGQSLSVLVTLDQVPKDYYIVASTRFTKIVLTATAVLHYSNSNSPV 300
           SHTLQNLYDSLDVHVGQSLSVLVTLDQ PKDYYIVASTRF++ VLTATAVLHYSNSNSPV
Sbjct: 241 SHTLQNLYDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRFSRTVLTATAVLHYSNSNSPV 300

Query: 301 SGPVPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSAPLIN 360
           SGP+PAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSAPLIN
Sbjct: 301 SGPLPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSAPLIN 360

Query: 361 GKQRYAINRVSYVNSDTPLKLADHFNIPGVFSVDSLQSLPSDGPAFIATSVLPTALHDFV 420
           GKQRYAINRVSYVNSDTPLKLAD+FNIPGVFSV+SLQSLPSDGPAFIATSVLPT LHDFV
Sbjct: 361 GKQRYAINRVSYVNSDTPLKLADYFNIPGVFSVNSLQSLPSDGPAFIATSVLPTFLHDFV 420

Query: 421 EIVFQNNENTVQSFHLDGYDFWVVGYGNGQWSQASRNSYNLVDALTRHTAQVYPNSWTTI 480
           EIVFQNNE+++QSFHLDGYDFWVVGYG GQWSQASRNSYNLVDALTRHTAQVYPNSWT I
Sbjct: 421 EIVFQNNEHSMQSFHLDGYDFWVVGYGYGQWSQASRNSYNLVDALTRHTAQVYPNSWTAI 480

Query: 481 LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPSNALLCGKAVGRH 538
           LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEY+IP NALLCGKA+GRH
Sbjct: 481 LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYSIPPNALLCGKAMGRH 537

BLAST of Spg013498 vs. TAIR 10
Match: AT5G66920.1 (SKU5 similar 17 )

HSP 1 Score: 842.8 bits (2176), Expect = 1.5e-244
Identity = 399/534 (74.72%), Postives = 454/534 (85.02%), Query Frame = 0

Query: 4   LGLLRLICAALALLSISLVNADNPYRFYTWKVTYGTISPLGVPQQVILINGQFPGPKLEV 63
           L  L L+ A   L S+ +V  ++PY+FYTW VTYG ISPLGVPQQVILINGQFPGPKLEV
Sbjct: 12  LNHLLLLGALTLLSSLVIVKGESPYKFYTWTVTYGIISPLGVPQQVILINGQFPGPKLEV 71

Query: 64  VTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYAFQTKDQIG 123
           VTNDNIILNLINKLD+PFLLTWNGIKQRKNSWQDGVLGTNC I PNSN+TY FQTKDQIG
Sbjct: 72  VTNDNIILNLINKLDQPFLLTWNGIKQRKNSWQDGVLGTNCPIQPNSNFTYKFQTKDQIG 131

Query: 124 SFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPIQDGDFTLLIGDWYKTSHKSLQETLDA 183
           +F YFPST FHKAAGGFGA+NVY RP IPIPYP+   DFTLL+GDW+KT+HK+LQ+ LD+
Sbjct: 132 TFNYFPSTAFHKAAGGFGAINVYARPGIPIPYPLPTADFTLLVGDWFKTNHKTLQQRLDS 191

Query: 184 GSSLPSPDGVLINGQSHSTFTGDQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEGSHT 243
           G  LP PDG+LINGQ+ STF+GDQGKTYM RISNVGLS++ NFRIQGH MK+VEVEGSH 
Sbjct: 192 GGVLPFPDGMLINGQTQSTFSGDQGKTYMLRISNVGLSSTFNFRIQGHTMKVVEVEGSHV 251

Query: 244 LQNLYDSLDVHVGQSLSVLVTLDQVPKDYYIVASTRFTKIVLTATAVLHYSNSNSPVSGP 303
           +Q  YDSLD+HVGQSL+VLVTL+Q PKDYYIVASTRF +  L+   +L YSNS  P SG 
Sbjct: 252 IQTDYDSLDIHVGQSLAVLVTLNQSPKDYYIVASTRFIRSKLSVMGLLRYSNSRVPASGD 311

Query: 304 VPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSAPLINGKQ 363
            PA P  E+ WS++QARTFRWNLTANAARPNPQGSFHYG I PTKT V + SAPLINGKQ
Sbjct: 312 PPALPPGELVWSMRQARTFRWNLTANAARPNPQGSFHYGMISPTKTFVFSNSAPLINGKQ 371

Query: 364 RYAINRVSYVNSDTPLKLADHFNIPGVFSVDSLQSLPSDGPAFIATSVLPTALHDFVEIV 423
           RYA+N VSYV S+TPLKLADHF I GVFS +++QS+PS+ P  +ATSV+ T+ HDF+EIV
Sbjct: 372 RYAVNGVSYVKSETPLKLADHFGISGVFSTNAIQSVPSNSPPTVATSVVQTSHHDFLEIV 431

Query: 424 FQNNENTVQSFHLDGYDFWVVGYGNGQWSQASRNSYNLVDALTRHTAQVYPNSWTTILVS 483
           FQNNE ++QS+HLDGYDFWVVG+G+GQW+ A R+ +NLVDALTRHT QVYP SWTTILVS
Sbjct: 432 FQNNEKSMQSWHLDGYDFWVVGFGSGQWTPAKRSLHNLVDALTRHTTQVYPESWTTILVS 491

Query: 484 LDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPSNALLCGKAVGRH 538
           LDNQGMWN+RSAIWERQY GQQFYL+VW   QSLANEYN P N  LCGKAVGRH
Sbjct: 492 LDNQGMWNMRSAIWERQYSGQQFYLKVWNSVQSLANEYNPPDNLQLCGKAVGRH 545

BLAST of Spg013498 vs. TAIR 10
Match: AT2G23630.1 (SKU5 similar 16 )

HSP 1 Score: 719.2 bits (1855), Expect = 2.5e-207
Identity = 346/536 (64.55%), Postives = 411/536 (76.68%), Query Frame = 0

Query: 4   LGLLRLICAALALLSISLVNADNPYRFYTWKVTYGTISPLGVPQQVILINGQFPGPKLEV 63
           LG L   C +    S+ ++NA++PY F+TW VTYGT SPLGVPQQVILINGQFPGP +E 
Sbjct: 9   LGFLLAYCFS----SVFVINAEDPYLFFTWTVTYGTRSPLGVPQQVILINGQFPGPPIEG 68

Query: 64  VTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYAFQTKDQIG 123
           VTN+NI++N+INKLDEPFL+TWNGIKQRK SWQDGVLGTNC I P S++TY FQ KDQIG
Sbjct: 69  VTNNNIVVNVINKLDEPFLITWNGIKQRKMSWQDGVLGTNCPIQPKSSWTYHFQLKDQIG 128

Query: 124 SFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPIQDGDFTLLIGDWYKTSHKSLQETLDA 183
           ++AYF ST  H+A+G FGALNV QR  I +PYP  D DFTLL+ DWYK  HK LQ  LD+
Sbjct: 129 TYAYFASTSMHRASGAFGALNVNQRSVIFVPYPKPDADFTLLVSDWYKMGHKELQRRLDS 188

Query: 184 GSSLPSPDGVLINGQSHS-TFTGDQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEGSH 243
             +LP PDG+LING S    FTG  GK Y FRISNVG+STS+NFRIQGH M LVEVEGSH
Sbjct: 189 SRALPPPDGLLINGASKGLVFTGQHGKIYRFRISNVGISTSINFRIQGHMMTLVEVEGSH 248

Query: 244 TLQNLYDSLDVHVGQSLSVLVTLDQVPKDYYIVASTRFTKIVLTATAVLHYSNSNSPVSG 303
           TLQ +Y+SLD+HVGQS++VLVTL    KDY+IVASTRFTK +LT T +L Y  S    S 
Sbjct: 249 TLQEVYESLDIHVGQSVTVLVTLKAPVKDYFIVASTRFTKPILTTTGILSYQGSKIRPSH 308

Query: 304 PVPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSAPLINGK 363
           P+P GPT  + WS+KQART R NLTANAARPNPQGSFHYG I   +T VLA    +INGK
Sbjct: 309 PLPIGPTYHIHWSMKQARTIRLNLTANAARPNPQGSFHYGTIPINRTFVLANGRAMINGK 368

Query: 364 QRYAINRVSYVNSDTPLKLADHFNIPGVFSVDSLQSLPSDGPAFIATSVLPTALHDFVEI 423
            RY +NRVSYVN  TPLKLAD FNIPGVF+  ++ ++P+ GP+ + TSV   ALH++VE 
Sbjct: 369 LRYTVNRVSYVNPATPLKLADWFNIPGVFNFKTIMNIPTPGPSILGTSVFDVALHEYVEF 428

Query: 424 VFQNNENTVQSFHLDGYDFWVVGYGNGQWSQASRNSYNLVDALTRHTAQVYPNSWTTILV 483
           VFQNNE ++QS+HLDG   +VVGYG+G W+ A R  YNLVDA++RHT QVYP SWT+ILV
Sbjct: 429 VFQNNEGSIQSWHLDGTSAYVVGYGSGTWNMAKRRGYNLVDAVSRHTFQVYPMSWTSILV 488

Query: 484 SLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPSNALLCGKAVGRHP 539
           SLDN+GMWNLRS IW R+YLGQ+ Y+RVW   +SL  E   P N L CGKA  +HP
Sbjct: 489 SLDNKGMWNLRSQIWSRRYLGQELYVRVWNNEKSLYTESEPPVNVLFCGKA--KHP 538

BLAST of Spg013498 vs. TAIR 10
Match: AT4G37160.1 (SKU5 similar 15 )

HSP 1 Score: 714.1 bits (1842), Expect = 8.2e-206
Identity = 345/537 (64.25%), Postives = 412/537 (76.72%), Query Frame = 0

Query: 1   MEKLGLL--RLICAALALLSISLVNADNPYRFYTWKVTYGTISPLGVPQQVILINGQFPG 60
           M++  LL  +L   AL  L   LVNA++PY FYTW VTYGT SPLGVPQQVILINGQFPG
Sbjct: 1   MKQTNLLVCKLFIGALFWLGSVLVNAEDPYMFYTWTVTYGTRSPLGVPQQVILINGQFPG 60

Query: 61  PKLEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYAFQT 120
           P +E VTN+NI++NLINKLDEPFL+TWNG+KQR+ SWQDGVLGTNC I PNSN+TY FQ 
Sbjct: 61  PAIEAVTNNNIVVNLINKLDEPFLITWNGVKQRRTSWQDGVLGTNCPIQPNSNWTYQFQL 120

Query: 121 KDQIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPIQDGDFTLLIGDWYKT-SHKSL 180
           KDQIG++ YF ST  H+A+G FGALN+ QR  I  PYP  DGDFTLL+ DW+   +HK L
Sbjct: 121 KDQIGTYTYFASTSLHRASGAFGALNINQRSVITTPYPTPDGDFTLLVSDWFSNMTHKDL 180

Query: 181 QETLDAGSSLPSPDGVLINGQSHS-TFTGDQGKTYMFRISNVGLSTSLNFRIQGHKMKLV 240
           +++LDAGS+LP PD +LING S    FTG QGKTY FR+SNVG++TS+NFRIQ H M L+
Sbjct: 181 RKSLDAGSALPLPDALLINGVSKGLIFTGQQGKTYKFRVSNVGIATSINFRIQNHTMSLI 240

Query: 241 EVEGSHTLQNLYDSLDVHVGQSLSVLVTLDQVPKDYYIVASTRFTKIVLTATAVLHYSNS 300
           EVEG+HTLQ  Y+SLDVHVGQS++VLVTL    +DY+IVASTRFTK VLT TA L Y  S
Sbjct: 241 EVEGAHTLQESYESLDVHVGQSMTVLVTLKASVRDYFIVASTRFTKPVLTTTASLRYQGS 300

Query: 301 NSPVSGPVPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSA 360
            +   GP+P GPT  + WS+KQART R NLTANAARPNPQGSFHYG I   +T+VLA +A
Sbjct: 301 KNAAYGPLPIGPTYHIHWSMKQARTIRMNLTANAARPNPQGSFHYGTIPINRTLVLANAA 360

Query: 361 PLINGKQRYAINRVSYVNSDTPLKLADHFNIPGVFSVDSLQSLPSDGPAFIATSVLPTAL 420
            LI GK RY +NR+SY+N  TPLKLAD +NI GVF   ++ S P+ GPA I TSV+   L
Sbjct: 361 TLIYGKLRYTVNRISYINPTTPLKLADWYNISGVFDFKTIISTPTTGPAHIGTSVIDVEL 420

Query: 421 HDFVEIVFQNNENTVQSFHLDGYDFWVVGYGNGQWSQASRNSYNLVDALTRHTAQVYPNS 480
           H+FVEIVFQN+E ++QS+H+DG   + VGYG+G W+   R  YNLVDA+ RHT QVYP S
Sbjct: 421 HEFVEIVFQNDERSIQSWHMDGTSAYAVGYGSGTWNVTMRKRYNLVDAVPRHTFQVYPLS 480

Query: 481 WTTILVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPSNALLCGKA 534
           WTTILVSLDN+GMWNLRS IW R+YLGQ+ Y+RVW   +SL  E   P N L CGKA
Sbjct: 481 WTTILVSLDNKGMWNLRSQIWSRRYLGQELYVRVWNDEKSLYTEAEPPLNVLYCGKA 537

BLAST of Spg013498 vs. TAIR 10
Match: AT4G22010.1 (SKU5 similar 4 )

HSP 1 Score: 708.8 bits (1828), Expect = 3.4e-204
Identity = 335/531 (63.09%), Postives = 413/531 (77.78%), Query Frame = 0

Query: 10  ICAALALLSISLVNADNPYRFYTWKVTYGTISPLGVPQQVILINGQFPGPKLEVVTNDNI 69
           +   L L+ I+ V  DNPYRF+TWK+TYG I PLGV QQ ILINGQFPGP ++ +TNDNI
Sbjct: 7   VSIVLLLVLINGVLGDNPYRFFTWKITYGDIYPLGVKQQGILINGQFPGPHIDAITNDNI 66

Query: 70  ILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYAFQTKDQIGSFAYFP 129
           I+++ N L EPFL++WNG++QRKNSWQDGV+GT C IPP  N+TY  Q KDQIGSF YFP
Sbjct: 67  IISVFNYLKEPFLISWNGVQQRKNSWQDGVVGTTCPIPPGKNFTYVIQVKDQIGSFYYFP 126

Query: 130 STQFHKAAGGFGALNVYQRPRIPIPYPIQDGDFTLLIGDWYKTSHKSLQETLDAGSSLPS 189
           S  FHKAAG FGA+ V+ RPRIP+P+   DGDF LL GDWYKT+H  L+  L+AG +LP+
Sbjct: 127 SLAFHKAAGAFGAIRVWSRPRIPVPFSPPDGDFWLLAGDWYKTNHYVLRRLLEAGRNLPN 186

Query: 190 PDGVLINGQ--SHSTFTGDQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEGSHTLQNL 249
           PDGVLING+    +TFT   GKTY FRISNVG++TSLNFRIQGH MKLVEVEGSHT+QN+
Sbjct: 187 PDGVLINGRGWGGNTFTVQPGKTYRFRISNVGVATSLNFRIQGHTMKLVEVEGSHTVQNI 246

Query: 250 YDSLDVHVGQSLSVLVTLDQVPKDYYIVASTRFTKIVLTATAVLHYSNSNSPVSGPVPAG 309
           Y SLD+H+GQS SVLVT +Q P+DYYIV S+RFT+ VLT T++LHYSNS   VSGPVP G
Sbjct: 247 YTSLDIHLGQSYSVLVTANQAPQDYYIVISSRFTRKVLTTTSILHYSNSRKGVSGPVPNG 306

Query: 310 PTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSAPLINGKQRYAI 369
           PT+++  S+ QART R NLTA+  RPNPQGS+HYG I P +TI+LA SAP INGKQRYA+
Sbjct: 307 PTLDIASSLYQARTIRRNLTASGPRPNPQGSYHYGLIKPGRTIILANSAPWINGKQRYAV 366

Query: 370 NRVSYVNSDTPLKLADHFNIPGVFSVDSLQSLPSDG-PAFIATSVLPTALHDFVEIVFQN 429
           N  S+V  DTPLKLAD+F IPGVF++ S+ + PS G   ++ +SV+     +F+E+VFQN
Sbjct: 367 NGASFVAPDTPLKLADYFKIPGVFNLGSIPTSPSGGNGGYLQSSVMAANFREFIEVVFQN 426

Query: 430 NENTVQSFHLDGYDFWVVGYGNGQWSQASRNSYNLVDALTRHTAQVYPNSWTTILVSLDN 489
            EN+VQS+H+ GY F+VVG   GQW+  SR  YNL DA++R T QVYP +WT I ++LDN
Sbjct: 427 WENSVQSWHVSGYSFFVVGMDGGQWTPGSRAKYNLRDAVSRSTVQVYPRAWTAIYIALDN 486

Query: 490 QGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPSNALLCGKAVGRH 538
            GMWN+RS  W RQYLGQQFYLRV+T + S  +EY  P NAL+CG+A GRH
Sbjct: 487 VGMWNIRSENWARQYLGQQFYLRVYTSSTSYRDEYPPPKNALMCGRAKGRH 537

BLAST of Spg013498 vs. TAIR 10
Match: AT1G76160.1 (SKU5 similar 5 )

HSP 1 Score: 693.0 bits (1787), Expect = 1.9e-199
Identity = 331/529 (62.57%), Postives = 398/529 (75.24%), Query Frame = 0

Query: 12  AALALLSISL---VNADNPYRFYTWKVTYGTISPLGVPQQVILINGQFPGPKLEVVTNDN 71
           AA   + +SL   V A++PYRF+ W +TYG I PLGV QQ ILING FPGP +  VTNDN
Sbjct: 8   AAALFIGLSLLFAVTAEDPYRFFEWNITYGDIYPLGVRQQGILINGAFPGPDIHSVTNDN 67

Query: 72  IILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYAFQTKDQIGSFAYF 131
           +I+N+ N LDEPFLL+WNGI+QR+NS+ DGV GT C IPP  NYTY  Q KDQIGSF YF
Sbjct: 68  LIINVYNSLDEPFLLSWNGIQQRRNSFVDGVYGTTCPIPPGKNYTYILQMKDQIGSFYYF 127

Query: 132 PSTQFHKAAGGFGALNVYQRPRIPIPYPIQDGDFTLLIGDWYKTSHKSLQETLDAGSSLP 191
           PS  FHKAAGGFG + +  RPRIP+P+P   GD T+LIGDWYK +H  L+  LD G  LP
Sbjct: 128 PSLGFHKAAGGFGGIRILSRPRIPVPFPDPAGDTTVLIGDWYKANHTDLRAQLDNGKKLP 187

Query: 192 SPDGVLINGQSH-STFTGDQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEGSHTLQNL 251
            PDG+LING+S  +T   +QGKTY FRISNVGL  SLNFRIQ HKMK+VEVEG+HTLQ  
Sbjct: 188 LPDGILINGRSSGATLNVEQGKTYRFRISNVGLQDSLNFRIQDHKMKVVEVEGTHTLQTT 247

Query: 252 YDSLDVHVGQSLSVLVTLDQVPKDYYIVASTRFTKIVLTATAVLHYSNSNSPVSGPVPAG 311
           + SLDVHVGQS SVLVT DQ P+DYY+V S+RFT  VLT T +  YSNS   VSGP+P G
Sbjct: 248 FSSLDVHVGQSYSVLVTADQTPRDYYVVVSSRFTSNVLTTTGIFRYSNSAGGVSGPIPGG 307

Query: 312 PTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSAPLINGKQRYAI 371
           PTI++DWS+ QAR  R NL+A+  RPNPQGS+HYG I  T+TI LA+SA  ++GKQRYA+
Sbjct: 308 PTIQIDWSLNQARAIRTNLSASGPRPNPQGSYHYGMINTTRTIRLASSAGQVDGKQRYAV 367

Query: 372 NRVSYVNSDTPLKLADHFNIPGVFSVDSLQSLPSDGPAFIATSVLPTALHDFVEIVFQNN 431
           N VS+  +DTPLK+AD+F I GV+   S+Q  P+ G  ++ TSV+      FVEI+F+N+
Sbjct: 368 NSVSFKPADTPLKIADYFKIDGVYRSGSIQYQPTGGGIYLDTSVMQVDYRTFVEIIFENS 427

Query: 432 ENTVQSFHLDGYDFWVVGYGNGQWSQASRNSYNLVDALTRHTAQVYPNSWTTILVSLDNQ 491
           E+ VQS+HLDGY FWVVG   GQWS  SRN YNL DA+ R T QVYP+SWT IL++LDN 
Sbjct: 428 EDIVQSWHLDGYSFWVVGMDGGQWSPDSRNEYNLRDAVARCTVQVYPSSWTAILIALDNV 487

Query: 492 GMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPSNALLCGKAVGR 537
           GMWNLRS  W RQYLGQQ YLRV+T + SL +EY IP NALLCG+A GR
Sbjct: 488 GMWNLRSEFWARQYLGQQLYLRVYTPSTSLRDEYPIPKNALLCGRASGR 536