Spg013448 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg013448
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Lipase_3 domain-containing protein
Location	scaffold1: 8719275 .. 8723969 (+)
RNA-Seq Expression	Spg013448
Synteny	Spg013448

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAGACTTGTTGCTCCATTTGTGGCAGCCCCCAAGAGAGATCTGAGATCTTGGTCATCAGGCATGGCTGCTGCTAGTGCCTGTAACTGTAACAAGGGCTTCTCCAACAGCTATTTGCTTCTGAAGCCGGAAGAAGTGAAGTTGTTGGACCTGCTGCGCCTCTTGTTTTCCAGTAACCTCAAGAAAAGAAAATTCGTCGATTCGAGCCATACGAGAGAATACAATTTCTGGCACAGATTCTTCATCTTTCTGTCTATTGTCGTCCTAAAGCTCCTCCGATTCTTCGCCACCCCACTCGCCTTGTTTGGCTTCTACCTCGAGTCCTGCCTCAACCTCTTGTCCCTCAACCACGGCTTTTCCGGCCTCCTCCTCAATATCTTAAGATGTAAGTCCCCTTTCATCTCATATTTTTTCTTACATACTTCAATTAACTTCGATCGAATCTCTCACTTCTTCGTGTTTATGTGTTTTTTAATTTTTTTTTTTTAAATGATGTTCTTTTCTTTGTTCAAGTTGCAGTTCGTCAAAGTAGAAAAATGTACTATCTTCAAAAAAAAAAAAAAAAAAAAAAGTACTCCGTATCTTGAACCTTTTTTGTATTATCATTAAAAAAAAATTGACAAATTTTATTGAGAAAAATACATTTTTGGTTCATAAGTTTTGAAGAATAGGTGCATTTGGTATCTAAATTTTCAAAATAGATTTTTTTAGTCTATAAGTTTTGAGAAATAGATTTAAAATGTCCCTAAATTTTCAAAATGAATCTTTTAGGGCCCGTTTGATAACCATTTCGTTTTCTGTTTTCTGTTTTTGTTTTTCATTTTTAAAAAACAGGAGTGTTTGATAACCATTTCTGTTTTCTGTTTTCTGTTTTTTAAAAACGTTTTTGAAATTATAAGTAAATTTCAAAAACCAAAAAAAGTAGTTTTTAAAAACAGATTTCTGTTTTTCAAAAACTCATTTTTCTTCTAATGCTTACTTTTCTCCTCTTTTACCTCTCTCTCCAACAAATTTCTTTCTCTATTTTTTTTTCTCTCTCTAAAATTTATTTTTCTCTCTCTAAAATTCTCTCTCAAAACTTTTTTTTTTCTCTCTCTAAAATTTATTTTTCTCTCTCTAAAATTCTCTCTCAAAACTTTTTTTTTCTCTATAACTTGTCTATTTCTAAAAACTTGTCAATATCTTTTCTCTCCTCAATAAACATGTTCTAATTTTTCTTTTGAAAAAAAAAAGTCACCAAGATTTTCTATCGCTACAACTTTTCTTTATTTTTCTAATTTATCGTTAGTTTTTTAGCATATCTTTTACTCTTAATTTTACTATCTCTCTAAATTTTATTTTTTCATTTAATTTTTTTAATCTAAAATTTATCGTTTTTACTCTAACTTGTTTTCTATTTTTTCTCTAACTAATATCTTTTCTCGAGTCTAACTAATATTCTTTCTCTAAACATTTTTTTTCATCTCTCTAAATTTTCTATGTTTAACTTTTCTCTCATCTTTTAAAAAAAAAAAATTCTATTGAAAACATTTATATTTGTGTCTCTAAATTTTTGTTTCAACGTCTTTACACATTTTTCTCTCTCTAAAATATCTTTTCATCTTTCTAATTTTTAAAAAACTGAAAACCAAAAACAGTTATCAAACAAGGTTGGTTTTCTGTTTTTTAAAAACAAAAAACAAAAAACAGTTATCAAACATATGTGGTTTTTGTTTTTAAAAAACAGGAAACCAAAAACCAAAAACCAAAAACAGAAAAACCAAAAACCAAAAACTGAAATGGTTATCAAACGGACCCTTAGTTTTTGAGTTTTGAGAAATATGTTTAAAATGTTCCTCATATTATTTTTTCAAAATTTTTAAAATAATATATATTTTTATTTTTTTATATTGAATGGTTTTTTAAATTAAAAAATTTTAAAAGTATTAATTTAAAAAAATAAAAATAATACGACCGACAATTTAAACTTATTTCTCAAGATTCGCAGACTAATTAAAAAGACCCATTTTGAAAACTTGGAGACCTTTTAAACATATTTCTCGAAACTCAAGAACTACAAAGGTCTATTTTAAAAATTCATGGACAAAATGCATCCATTCCTCAAAACTCAGATACTAAAAATGTATTTTTTTTTCTAATTTTATTTATTTATTTACTTTTAAAAATATCTCCCTTTCTCTCTCCAACTTGTCAAAATATTAATGTTGCCAACAAAAATTGTTGGCATCTCTGGCTTAACCAATCAATAAGTAAAATATTATTTGTTTAAAAGTACTGATAAAATAATTTCTTATTACTTGACTAGTCAGCAGAGAAATATCTTCATACTTACCGTATATTATTTAAGGATTGCATGGCAGGTTAATTTTGAACATGTTCAAACACAATGAACATAGTTATGAGTTAACACAATGAACTATATGTTAAAGATTCTTTTTTTTTTCTTTGATATCAAAGATATATTAAAGATGTATTAAAAACTTCTTAAAATACTAAACTTCTATAAACACAATCTTTAAAAGTTAGACATATTTGAATTTTGAAAATTACGGGATATAGTGGTGAAAGTTTAAAAAGTAAATTATTTCTGATTTAAGTTGTGCTCGGGGTTGATTTTAATTAAAAGAGATAGATGTGATTAGAAAAGAATTTTAACAGTTTTATGCATTTGGTAAAATAGATTATAAATTAATTTTAATAAAGATACTTTTCAACGGTTGATTTGCAAAATCTAATAATGGCAGCTTTCTAGCTTTTCTAAAAATTACACTTGAGACATAATTCACCATCAATCATTTTTTTATTTGAAGTTAATTTATTTTTTTCTATTATGTTATATTGAATTGAATTTATTTGTCTTTATTTTTTTATTTAAATTGAAATAACATTTTTTTTCAACATGACTTGTATCGAACTATAAAAATATCCTAGATTAAACACAATATTAAATTTTAAATCATATGAAATTTAAAATAATTATCTTATAGTAATTTTGAAATTATCTTATAAAAATTTGAATTAAATATGAATGGAATAATTATACCTATTTTGATTATTATAATTGAGCTACAACAATTTAGAATATTAATTTACCAAACATCTTAACTTGTTTTTTTCATCTATAAAACTAAAATTTATCAAACACTAATGTATTTTCTAATAAGTTGATTTTCTTAAAAACACATTTGACAACAATAACTTTAGAAGCTACCGAACGGCATAATAAATTAACACCCTGAAGAAAGGATTGAAATAAATAAATATATATCCTTGTTTTGTTATGCAGTGAGGGTGAAAAAACCGGACAGCACATCGGCGGAGTACCTGTCGATGATTGGTCATTTGGATAGCAGAGTAATGTTAGACAAAAATATAAAGCCTGGCGATGTCAACTACTTTGGGGCTCTCTGCATGATGGCTTCTAAAGTGGTCTATGAGAATGAAGCTCACGTTACTCAAATTGTTAAGGATGTCTGGAAGGTACACTACACTCCACCACTAATTACCACTTTTATATTTAAATTATTTTCTCACTTATTAACTAATTTTTCCTTTTAATGTTTTCTAGATGGAGTTCTTGGGATTTTTCAACTTTTGGAACGGTAATTTTTCCTTTTCCATTTTGCTAGTCAGAAGTGCATAAGTAATATAATGATACTTTTAATTGTATAAACTAGATTATCAAGAAACATGCTCTACACAAGCGTTCATGATGCGGGATAAAAAAACTGATCACGACACCATCATCGTGTCCTTCAGAGGCACAGAGCCCTTCAATGCCGACGACTGGAGCTCTGACTTCGACATTTCTTGGTACGAGATCCAAGGCATCGGAAAGATCCATGGCGGATTCATGAAAGCACTTGGCTTGCAGAAAACCTTCGGATGGCCCAAAGAAATAGCGCGACAAGATAGAAGACGACCTTTGGCTTACTACACCCTCAGAGAAAGGCTTAGAGAACTTCTCAAAGAAAATGAGCGAACGAGGTTCGTAGTGACTGGGCACAGCCTGGGCGGCGCTTTGGCCATCCTATTTCCTTCTATTCTGGCCTTCCATGAGGAAAAGTTACTGCTGGAAAGATTAGAGGGAGTGTACACTTTTGGACAACCTCGTGTTGGAGACGGAAAATTTGGAGAATTCATGGTGAAATCACTCTCGCAGTACAAGATTCGTTACTACAGATTTGTTTACGGTTTCGACATGGTCCCTCGGCTGCCCCTTGACGACAAGGCCTTGATGTTCAAACACTTCGGACCTTGTATTTATTTCGACTGGAACTATGTTGCGCAGGTAAAAGCTAACATTTTTATATATACATATCTATTGAAGAAACGTTTTTATTTGAATGAAACCCTTTTAATAATTTAGTGGGTGGTAATTAAATTTTTTGGGTGTTGGCAGATTCTTGAAGAAGAGCCATTCAAGAACTATTTCTCGATAGTGGGAGCCGTTCTGATGAGAATCCATGCATGTTTCGAGATAGGAAGAAGCTTCACGATTTCATGGAGAAGGGGAAAGGAGTACGAGGAAAGAGTGATGTTGAGAATTATGAGGTTGTTTGGATTGTTATTGCCTGGAATTCCTGCACATTGTCCTCAAGATTATGTTAATTCAACTCGCTTGGCTTCCACAGACATCTTCTTTTCTCCTCCCAAAGGTCGTTTTCACAGCGAGTACGATGTCAAGATTCACTAG

mRNA sequence

ATGGCTGCTGCTAGTGCCTGTAACTGTAACAAGGGCTTCTCCAACAGCTATTTGCTTCTGAAGCCGGAAGAAGTGAAGTTGTTGGACCTGCTGCGCCTCTTGTTTTCCAGTAACCTCAAGAAAAGAAAATTCGTCGATTCGAGCCATACGAGAGAATACAATTTCTGGCACAGATTCTTCATCTTTCTGTCTATTGTCGTCCTAAAGCTCCTCCGATTCTTCGCCACCCCACTCGCCTTGTTTGGCTTCTACCTCGAGTCCTGCCTCAACCTCTTGTCCCTCAACCACGGCTTTTCCGGCCTCCTCCTCAATATCTTAAGATTGAGGGTGAAAAAACCGGACAGCACATCGGCGGAGTACCTGTCGATGATTGGTCATTTGGATAGCAGAGTAATGTTAGACAAAAATATAAAGCCTGGCGATGTCAACTACTTTGGGGCTCTCTGCATGATGGCTTCTAAAGTGGTCTATGAGAATGAAGCTCACGTTACTCAAATTGTTAAGGATGTCTGGAAGATGGAGTTCTTGGGATTTTTCAACTTTTGGAACGATTATCAAGAAACATGCTCTACACAAGCGTTCATGATGCGGGATAAAAAAACTGATCACGACACCATCATCGTGTCCTTCAGAGGCACAGAGCCCTTCAATGCCGACGACTGGAGCTCTGACTTCGACATTTCTTGGTACGAGATCCAAGGCATCGGAAAGATCCATGGCGGATTCATGAAAGCACTTGGCTTGCAGAAAACCTTCGGATGGCCCAAAGAAATAGCGCGACAAGATAGAAGACGACCTTTGGCTTACTACACCCTCAGAGAAAGGCTTAGAGAACTTCTCAAAGAAAATGAGCGAACGAGGTTCGTAGTGACTGGGCACAGCCTGGGCGGCGCTTTGGCCATCCTATTTCCTTCTATTCTGGCCTTCCATGAGGAAAAGTTACTGCTGGAAAGATTAGAGGGAGTGTACACTTTTGGACAACCTCGTGTTGGAGACGGAAAATTTGGAGAATTCATGGTGAAATCACTCTCGCAGTACAAGATTCGTTACTACAGATTTGTTTACGGTTTCGACATGGTCCCTCGGCTGCCCCTTGACGACAAGGCCTTGATGTTCAAACACTTCGGACCTTGTATTTATTTCGACTGGAACTATGTTGCGCAGATTCTTGAAGAAGAGCCATTCAAGAACTATTTCTCGATAGTGGGAGCCGTTCTGATGAGAATCCATGCATGTTTCGAGATAGGAAGAAGCTTCACGATTTCATGGAGAAGGGGAAAGGAGTACGAGGAAAGAGTGATGTTGAGAATTATGAGGTTGTTTGGATTGTTATTGCCTGGAATTCCTGCACATTGTCCTCAAGATTATGTTAATTCAACTCGCTTGGCTTCCACAGACATCTTCTTTTCTCCTCCCAAAGGTCGTTTTCACAGCGAGTACGATGTCAAGATTCACTAG

Coding sequence (CDS)

ATGGCTGCTGCTAGTGCCTGTAACTGTAACAAGGGCTTCTCCAACAGCTATTTGCTTCTGAAGCCGGAAGAAGTGAAGTTGTTGGACCTGCTGCGCCTCTTGTTTTCCAGTAACCTCAAGAAAAGAAAATTCGTCGATTCGAGCCATACGAGAGAATACAATTTCTGGCACAGATTCTTCATCTTTCTGTCTATTGTCGTCCTAAAGCTCCTCCGATTCTTCGCCACCCCACTCGCCTTGTTTGGCTTCTACCTCGAGTCCTGCCTCAACCTCTTGTCCCTCAACCACGGCTTTTCCGGCCTCCTCCTCAATATCTTAAGATTGAGGGTGAAAAAACCGGACAGCACATCGGCGGAGTACCTGTCGATGATTGGTCATTTGGATAGCAGAGTAATGTTAGACAAAAATATAAAGCCTGGCGATGTCAACTACTTTGGGGCTCTCTGCATGATGGCTTCTAAAGTGGTCTATGAGAATGAAGCTCACGTTACTCAAATTGTTAAGGATGTCTGGAAGATGGAGTTCTTGGGATTTTTCAACTTTTGGAACGATTATCAAGAAACATGCTCTACACAAGCGTTCATGATGCGGGATAAAAAAACTGATCACGACACCATCATCGTGTCCTTCAGAGGCACAGAGCCCTTCAATGCCGACGACTGGAGCTCTGACTTCGACATTTCTTGGTACGAGATCCAAGGCATCGGAAAGATCCATGGCGGATTCATGAAAGCACTTGGCTTGCAGAAAACCTTCGGATGGCCCAAAGAAATAGCGCGACAAGATAGAAGACGACCTTTGGCTTACTACACCCTCAGAGAAAGGCTTAGAGAACTTCTCAAAGAAAATGAGCGAACGAGGTTCGTAGTGACTGGGCACAGCCTGGGCGGCGCTTTGGCCATCCTATTTCCTTCTATTCTGGCCTTCCATGAGGAAAAGTTACTGCTGGAAAGATTAGAGGGAGTGTACACTTTTGGACAACCTCGTGTTGGAGACGGAAAATTTGGAGAATTCATGGTGAAATCACTCTCGCAGTACAAGATTCGTTACTACAGATTTGTTTACGGTTTCGACATGGTCCCTCGGCTGCCCCTTGACGACAAGGCCTTGATGTTCAAACACTTCGGACCTTGTATTTATTTCGACTGGAACTATGTTGCGCAGATTCTTGAAGAAGAGCCATTCAAGAACTATTTCTCGATAGTGGGAGCCGTTCTGATGAGAATCCATGCATGTTTCGAGATAGGAAGAAGCTTCACGATTTCATGGAGAAGGGGAAAGGAGTACGAGGAAAGAGTGATGTTGAGAATTATGAGGTTGTTTGGATTGTTATTGCCTGGAATTCCTGCACATTGTCCTCAAGATTATGTTAATTCAACTCGCTTGGCTTCCACAGACATCTTCTTTTCTCCTCCCAAAGGTCGTTTTCACAGCGAGTACGATGTCAAGATTCACTAG

Protein sequence

MAAASACNCNKGFSNSYLLLKPEEVKLLDLLRLLFSSNLKKRKFVDSSHTREYNFWHRFFIFLSIVVLKLLRFFATPLALFGFYLESCLNLLSLNHGFSGLLLNILRLRVKKPDSTSAEYLSMIGHLDSRVMLDKNIKPGDVNYFGALCMMASKVVYENEAHVTQIVKDVWKMEFLGFFNFWNDYQETCSTQAFMMRDKKTDHDTIIVSFRGTEPFNADDWSSDFDISWYEIQGIGKIHGGFMKALGLQKTFGWPKEIARQDRRRPLAYYTLRERLRELLKENERTRFVVTGHSLGGALAILFPSILAFHEEKLLLERLEGVYTFGQPRVGDGKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLPLDDKALMFKHFGPCIYFDWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFTISWRRGKEYEERVMLRIMRLFGLLLPGIPAHCPQDYVNSTRLASTDIFFSPPKGRFHSEYDVKIH

Homology

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BLAST of Spg013448 vs. NCBI nr
Match: KAG6595033.1 (Lipase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027056.1 Lipase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 864.4 bits (2232), Expect = 4.7e-247
Identity = 420/484 (86.78%), Postives = 444/484 (91.74%), Query Frame = 0


Query: 1   MAAASACNCNKGFSNSYLLLKPEEVKLLDLLRLLFSSNLKKRKFVDSSHTREYNFWHRFF 60
           MAA  A NCN GFS+SY+LLKPEEVK LDL RLLFSSNLKKR+F+DSSH REYNFWHRFF
Sbjct: 1   MAARRAYNCNNGFSDSYMLLKPEEVKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFF 60

Query: 61  IFLSIVVLKLLRFFATPLALFGFYLESCLNLLSLNHGFSGLLLNILRLRVKKPDSTSAEY 120
           IFLSIVVLKLL F A PLALFGFYLES LN+LS NHGF G+LLNILRL++K PDS+SAEY
Sbjct: 61  IFLSIVVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY 120

Query: 121 LSMIGHLDSRVMLDKNIKPGDVNYFGALCMMASKVVYENEAHVTQIVKDVWKMEFLGFFN 180
           LS+IGHLDSRV LD++IKPGDVNYFGALCMMASKVVYENEAHV Q V DVWKMEFLGFFN
Sbjct: 121 LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLGFFN 180

Query: 181 FWNDYQETCSTQAFMMRDKKTDHDTIIVSFRGTEPFNADDWSSDFDISWYEIQGIGKIHG 240
           FWN+YQE CSTQAFMMRDK+ DHDTIIVSFRGTEPFNADDWSSDFDISWYEI+GIGKIHG
Sbjct: 181 FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG 240

Query: 241 GFMKALGLQKTFGWPKEIARQDRRRPLAYYTLRERLRELLKENERTRFVVTGHSLGGALA 300
           GFMKALGLQKT GWPKEI R+D+RRPLAYYTLRERLRELLKE+ERTRFVVTGHSLGGALA
Sbjct: 241 GFMKALGLQKTHGWPKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA 300

Query: 301 ILFPSILAFHEEKLLLERLEGVYTFGQPRVGDGKFGEFMVKSLSQYKIRYYRFVYGFDMV 360
           ILFPSILAFHEEKLLLERLEGVYTFGQPRVGD KFGEFMVKSLSQYKIRYYRFVYGFDMV
Sbjct: 301 ILFPSILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV 360

Query: 361 PRLPLDDKALMFKHFGPCIYFDWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT 420
           PRLPLD+  LM+KHFGPCIYF+WNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT
Sbjct: 361 PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT 420

Query: 421 ISWRRGKEYEERVMLRIMRLFGLLLPGIPAHCPQDYVNSTRLASTDIFFSPPKGRFHSEY 480
           I WRRGKEYEER+  RI+RL GLLLPGIPAHCPQDYVNSTRL ST        G  +SEY
Sbjct: 421 IGWRRGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGST--------GSLNSEY 476

Query: 481 DVKI 485
           DVKI
Sbjct: 481 DVKI 476

[Close]

BLAST of Spg013448 vs. NCBI nr
Match: XP_023518931.1 (uncharacterized protein LOC111782304 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 862.1 bits (2226), Expect = 2.3e-246
Identity = 419/484 (86.57%), Postives = 443/484 (91.53%), Query Frame = 0


Query: 1   MAAASACNCNKGFSNSYLLLKPEEVKLLDLLRLLFSSNLKKRKFVDSSHTREYNFWHRFF 60
           MAA  A NCN GFS+SY+LLKPEEVK LDL RLLFSSNLKKR+F+DSS  REYNFWHRFF
Sbjct: 1   MAARRAYNCNNGFSDSYMLLKPEEVKFLDLWRLLFSSNLKKRRFLDSSRAREYNFWHRFF 60

Query: 61  IFLSIVVLKLLRFFATPLALFGFYLESCLNLLSLNHGFSGLLLNILRLRVKKPDSTSAEY 120
           IFLSIVVLKLL F A PLALFGFYLES LN+LS NHGF G+LLNILRL++K PDS+SAEY
Sbjct: 61  IFLSIVVLKLLGFLANPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY 120

Query: 121 LSMIGHLDSRVMLDKNIKPGDVNYFGALCMMASKVVYENEAHVTQIVKDVWKMEFLGFFN 180
           LS+IGHLDSRV LD++IKPGDVNYFGALCMMASKVVYENEAHV Q V DVWKMEFLGFFN
Sbjct: 121 LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLGFFN 180

Query: 181 FWNDYQETCSTQAFMMRDKKTDHDTIIVSFRGTEPFNADDWSSDFDISWYEIQGIGKIHG 240
           FWN+YQE CSTQAFMMRDK+ DHDTIIVSFRGTEPFNADDWSSDFDISWYEI+GIGKIHG
Sbjct: 181 FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG 240

Query: 241 GFMKALGLQKTFGWPKEIARQDRRRPLAYYTLRERLRELLKENERTRFVVTGHSLGGALA 300
           GFMKALGLQKT GWPKEI R+D+RRPLAYYTLRERLRELLKE+ERTRFVVTGHSLGGALA
Sbjct: 241 GFMKALGLQKTHGWPKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA 300

Query: 301 ILFPSILAFHEEKLLLERLEGVYTFGQPRVGDGKFGEFMVKSLSQYKIRYYRFVYGFDMV 360
           ILFPSILAFHEEKLLLERLEGVYTFGQPRVGD KFGEFMVKSLSQYKIRYYRFVYGFDMV
Sbjct: 301 ILFPSILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV 360

Query: 361 PRLPLDDKALMFKHFGPCIYFDWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT 420
           PRLPLD+  LM+KHFGPCIYF+WNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT
Sbjct: 361 PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT 420

Query: 421 ISWRRGKEYEERVMLRIMRLFGLLLPGIPAHCPQDYVNSTRLASTDIFFSPPKGRFHSEY 480
           I WRRGKEYEER+  RI+RL GLLLPGIPAHCPQDYVNSTRL ST        G  +SEY
Sbjct: 421 IGWRRGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGST--------GSLNSEY 476

Query: 481 DVKI 485
           DVKI
Sbjct: 481 DVKI 476

[Close]

BLAST of Spg013448 vs. NCBI nr
Match: XP_022962880.1 (uncharacterized protein LOC111463247 [Cucurbita moschata])

HSP 1 Score: 857.8 bits (2215), Expect = 4.4e-245
Identity = 417/484 (86.16%), Postives = 442/484 (91.32%), Query Frame = 0


Query: 1   MAAASACNCNKGFSNSYLLLKPEEVKLLDLLRLLFSSNLKKRKFVDSSHTREYNFWHRFF 60
           MAA  A NCN GFS+SY+LLKPEE K LDL RLLFSSNLKKR+F+DSSH REYNFWHRFF
Sbjct: 1   MAARRAYNCNNGFSDSYMLLKPEEEKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFF 60

Query: 61  IFLSIVVLKLLRFFATPLALFGFYLESCLNLLSLNHGFSGLLLNILRLRVKKPDSTSAEY 120
           IFLSI+VLKLL F A PLALFGFYLES LN+LS NHGF G+LLNILRL++K PDS+SAEY
Sbjct: 61  IFLSILVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY 120

Query: 121 LSMIGHLDSRVMLDKNIKPGDVNYFGALCMMASKVVYENEAHVTQIVKDVWKMEFLGFFN 180
           LS+IGHLDSRV LD++IKPGDVNYFGALCMMASKVVYENEAHV Q V DVWKMEFL FFN
Sbjct: 121 LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLEFFN 180

Query: 181 FWNDYQETCSTQAFMMRDKKTDHDTIIVSFRGTEPFNADDWSSDFDISWYEIQGIGKIHG 240
           FWN+YQE CSTQAFMMRDK+ DHDTIIVSFRGTEPFNADDWSSDFDISWYEI+GIGKIHG
Sbjct: 181 FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG 240

Query: 241 GFMKALGLQKTFGWPKEIARQDRRRPLAYYTLRERLRELLKENERTRFVVTGHSLGGALA 300
           GFMKALGLQKT GWPKEI R+D+RRPLAYYTLRERLRELLKE+ERTRFVVTGHSLGGALA
Sbjct: 241 GFMKALGLQKTHGWPKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA 300

Query: 301 ILFPSILAFHEEKLLLERLEGVYTFGQPRVGDGKFGEFMVKSLSQYKIRYYRFVYGFDMV 360
           ILFPSILAFHEEKLLLERLEGVYTFGQPRVGD KFGEFMVKSLSQYKIRYYRFVYGFDMV
Sbjct: 301 ILFPSILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV 360

Query: 361 PRLPLDDKALMFKHFGPCIYFDWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT 420
           PRLPLD+  LM+KHFGPCIYF+WNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT
Sbjct: 361 PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT 420

Query: 421 ISWRRGKEYEERVMLRIMRLFGLLLPGIPAHCPQDYVNSTRLASTDIFFSPPKGRFHSEY 480
           I WRRGKEYEER+  RI+RL GLLLPGIPAHCPQDYVNSTRL ST        G  +SEY
Sbjct: 421 IGWRRGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGST--------GSLNSEY 476

Query: 481 DVKI 485
           DVKI
Sbjct: 481 DVKI 476

[Close]

BLAST of Spg013448 vs. NCBI nr
Match: XP_008440283.1 (PREDICTED: uncharacterized protein LOC107990286 [Cucumis melo])

HSP 1 Score: 856.7 bits (2212), Expect = 9.9e-245
Identity = 417/479 (87.06%), Postives = 437/479 (91.23%), Query Frame = 0


Query: 8   NCNKGFSNSYLLLKPEEVKLLDLLRLLFSSNLKKRKFVDSSHTREYNFWHRFFIFLSIVV 67
           NCN GFS+SY+LL PEEVK LDLLRLLFSSNLKKR+FVDSSH RE+NFWHRFFIFLSIVV
Sbjct: 18  NCNGGFSSSYMLLNPEEVKFLDLLRLLFSSNLKKRRFVDSSHAREHNFWHRFFIFLSIVV 77

Query: 68  LKLLRFFATPLALFGFYLESCLNLLSLNHGFSGLLLNILRLRVKKPDSTSAEYLSMIGHL 127
           LKLLRFF  PLAL GF+LES LN LSLN GFSG+LLN  RLR+K PDS+SAEYLSMIGHL
Sbjct: 78  LKLLRFFDKPLALLGFFLESSLNFLSLNGGFSGILLNFFRLRLKIPDSSSAEYLSMIGHL 137

Query: 128 DSRVMLDKNIKPGDVNYFGALCMMASKVVYENEAHVTQIVKDVWKMEFLGFFNFWNDYQE 187
           DSRV LD +IKPGDVNYFGALCMMASK+ YENEAHV QIVKDVWKMEFLGFFNFWNDYQE
Sbjct: 138 DSRVTLDSSIKPGDVNYFGALCMMASKLSYENEAHVAQIVKDVWKMEFLGFFNFWNDYQE 197

Query: 188 TCSTQAFMMRDKKTDHDTIIVSFRGTEPFNADDWSSDFDISWYEIQGIGKIHGGFMKALG 247
            CSTQAFMMRDKK DHDTI+VSFRGTEPFNADDWSSDFDISWYEI+GIGKIHGGFMKALG
Sbjct: 198 KCSTQAFMMRDKKVDHDTIVVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKALG 257

Query: 248 LQKTFGWPKEIARQ--DRRRPLAYYTLRERLRELLKENERTRFVVTGHSLGGALAILFPS 307
           LQK  GWPKEI RQ  D RRPLAYYTLRE+L+EL+KENERTRFVVTGHSLGGALAILFPS
Sbjct: 258 LQKCIGWPKEIERQGHDERRPLAYYTLREKLKELVKENERTRFVVTGHSLGGALAILFPS 317

Query: 308 ILAFHEEKLLLERLEGVYTFGQPRVGDGKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLPL 367
           IL FHEEKLLLERLEGVYTFGQPRVGD  FGEFMVKSL+QYKIRYYRFVYGFDMVPRLPL
Sbjct: 318 ILVFHEEKLLLERLEGVYTFGQPRVGDRAFGEFMVKSLAQYKIRYYRFVYGFDMVPRLPL 377

Query: 368 DDKALMFKHFGPCIYFDWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFTISWRR 427
           DDKALMFKHFGPCIYFDWNYVAQILEEEPFKNYFSIVGA+LMRIHAC EIGRSFTISWRR
Sbjct: 378 DDKALMFKHFGPCIYFDWNYVAQILEEEPFKNYFSIVGAILMRIHACLEIGRSFTISWRR 437

Query: 428 GKEYEERVMLRIMRLFGLLLPGIPAHCPQDYVNSTRLASTDIFFSPPKGRFHSEYDVKI 485
           GKEYEE+V LRI+RLFGLLLPGIPAHCPQDYVNSTRL ST          F SEYDVK+
Sbjct: 438 GKEYEEKVFLRIVRLFGLLLPGIPAHCPQDYVNSTRLGST--------SHFLSEYDVKV 488

[Close]

BLAST of Spg013448 vs. NCBI nr
Match: XP_023003657.1 (uncharacterized protein LOC111497186 [Cucurbita maxima])

HSP 1 Score: 855.9 bits (2210), Expect = 1.7e-244
Identity = 416/484 (85.95%), Postives = 443/484 (91.53%), Query Frame = 0


Query: 1   MAAASACNCNKGFSNSYLLLKPEEVKLLDLLRLLFSSNLKKRKFVDSSHTREYNFWHRFF 60
           MAA  A NC+ GFS+SY+LLKPEEVK LDL RLLFSSNLKKR+F+DSSH REYNFWHRFF
Sbjct: 1   MAARRAYNCSNGFSDSYMLLKPEEVKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFF 60

Query: 61  IFLSIVVLKLLRFFATPLALFGFYLESCLNLLSLNHGFSGLLLNILRLRVKKPDSTSAEY 120
           IFLSIVVLKLL F A PLALFGFYLES LN+LS NHGF G+LLNILRL++K PDS+SAEY
Sbjct: 61  IFLSIVVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY 120

Query: 121 LSMIGHLDSRVMLDKNIKPGDVNYFGALCMMASKVVYENEAHVTQIVKDVWKMEFLGFFN 180
           LS+IGHLDSRV LD++IKPGDVNYFGALCMMASKVVYENEAHV Q V DVWKMEFLGFFN
Sbjct: 121 LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLGFFN 180

Query: 181 FWNDYQETCSTQAFMMRDKKTDHDTIIVSFRGTEPFNADDWSSDFDISWYEIQGIGKIHG 240
           FWN+YQE CSTQAFMMRDK+ DHDTIIVSFRGTEPFNADDWSSDFDISWYEI+GIGKIHG
Sbjct: 181 FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG 240

Query: 241 GFMKALGLQKTFGWPKEIARQDRRRPLAYYTLRERLRELLKENERTRFVVTGHSLGGALA 300
           GFMKALGLQKT GW KEI R+D+RRPLAYYTLRERLRELLKE+ERTRFVVTGHSLGGALA
Sbjct: 241 GFMKALGLQKTHGWTKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA 300

Query: 301 ILFPSILAFHEEKLLLERLEGVYTFGQPRVGDGKFGEFMVKSLSQYKIRYYRFVYGFDMV 360
           ILFP+ILAFHEEKLLLERLEGVYTFGQPRVGD KFGEFMVKSLSQYKIRYYRFVYGFDMV
Sbjct: 301 ILFPTILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV 360

Query: 361 PRLPLDDKALMFKHFGPCIYFDWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT 420
           PRLPLD+  LM+KHFGPCIYF+WNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT
Sbjct: 361 PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT 420

Query: 421 ISWRRGKEYEERVMLRIMRLFGLLLPGIPAHCPQDYVNSTRLASTDIFFSPPKGRFHSEY 480
           I WRRGKEYEER+  RI+RLFGLLLPGIPAHCPQDYVN+TRL ST           +SEY
Sbjct: 421 IGWRRGKEYEERMCFRIVRLFGLLLPGIPAHCPQDYVNATRLGST--------VSLNSEY 476

Query: 481 DVKI 485
           DVKI
Sbjct: 481 DVKI 476

[Close]

BLAST of Spg013448 vs. ExPASy Swiss-Prot
Match: A0A1S3ZP85 (Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 1.8e-100
Identity = 208/477 (43.61%), Postives = 277/477 (58.07%), Query Frame = 0


Query: 57  HRFFIFLSIVVLKLLRFFATPLALFGFYLESCLNLLSLNHGFSGLLLNILRLRVKKPDST 116
           HR+ IF+SI+V KL+  F  P+   G+ +E  LNL SLN  F GLL NIL  +V  P   
Sbjct: 85  HRWVIFVSIIVRKLIAIFGKPMEWTGYLVEFFLNLFSLNGNFLGLLYNILHGKVVMPHRG 144

Query: 117 SAEYLSMIGHLDSRVMLDKN----IKPGDVNYFGA----------LCMMASKVVYENEAH 176
           S  ++S IGHLD R+ L K+     + G+ +++            LCMMASK+ YENE  
Sbjct: 145 SETFISAIGHLDGRINLYKSETLTKEIGEPDFWQKIGIGHRDLMDLCMMASKLAYENEKV 204

Query: 177 VTQIVKDVWKMEFLGFFNFWNDYQETCSTQAFMMRDKKTDHDTIIVSFRGTEPFNADDWS 236
           V  +V   WKM F+ F+N WND+++  STQ F++ DK  D + I+VSFRGTEPF+ADDW 
Sbjct: 205 VRNVVNLHWKMHFVDFYNCWNDFEKEMSTQVFLLCDKPKDANLILVSFRGTEPFDADDWI 264

Query: 237 SDFDISWYEIQGIGKIHGGFMKALGL-----QKTFGW--------------------PKE 296
           +DFD SWYEI  +GK+H GF++ALGL       TF                      P E
Sbjct: 265 TDFDYSWYEIPKLGKVHMGFLEALGLGNRTNASTFHEQLFVNNLKFANLENVHATIPPSE 324

Query: 297 IAR-----------------QDRRRP---------------LAYYTLRERLRELLKENER 356
            ++                  D  RP                AYY +R +L+ LLKE++ 
Sbjct: 325 SSKSSTSFSDSDAHTGSDLSSDSERPTDTRKKKFRLEIPERTAYYVVRSKLKRLLKEHKN 384

Query: 357 TRFVVTGHSLGGALAILFPSILAFHEEKLLLERLEGVYTFGQPRVGDGKFGEFMVKSLSQ 416
            +FVVTGHSLGGALAILFP++L  HEE  ++ERL G+YT+GQPRVG+ + G FM   L  
Sbjct: 385 AKFVVTGHSLGGALAILFPAVLVLHEEVDVMERLLGIYTYGQPRVGNRQLGRFMEAHLEH 444

Query: 417 YKIRYYRFVYGFDMVPRLPLDDKALMFKHFGPCIYFDWNYVAQILEEEPFKNYFSIVGAV 463
              +Y+R VY  D+VPRLP D+K  +FKHFG C Y++  Y+ Q + EEP  NYF +   V
Sbjct: 445 PVPKYFRVVYCNDLVPRLPYDNKTFLFKHFGICQYYNSLYIEQNINEEPNPNYFGMRFLV 504

[Close]

BLAST of Spg013448 vs. ExPASy Swiss-Prot
Match: F4JFU8 (Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 9.0e-100
Identity = 205/501 (40.92%), Postives = 294/501 (58.68%), Query Frame = 0


Query: 5   SACNCNKGFSNSYLLLKPEEVKLLDLLRL-LFSSNLKKRKFVDSSHTREYNFW------- 64
           S    N G  ++YL+++P     +DL R  +      K KF++    RE++         
Sbjct: 7   SGSGSNPGQVSNYLIVRPHRGGYIDLFRYGVRDDQTSKAKFLEMPDNREWSTITIDEEAE 66

Query: 65  -HRFFIFLSIVVLKLLRFFATPLALFGFYLESCLNLLSLNHGFSGLLLNILRLRVKKPDS 124
            HR+ I +SI+V K++R   TP+   GF ++  LNL S N GF GLLL +++ +V  P+ 
Sbjct: 67  DHRWVIVVSILVRKIIRLLRTPMEFTGFVVDFFLNLFSANGGFFGLLLRLIQAKVVIPER 126

Query: 125 TSAEYLSMIGHLDSRVMLDK------------NIKPGDVNY-FGA-----LCMMASKVVY 184
            S  ++S IG LD R+ L K            ++  G V    G+     LC+MASK+ Y
Sbjct: 127 GSVTFVSTIGQLDGRISLYKEWNFVEHLEGIDSVDSGRVKIELGSRGLMDLCVMASKLAY 186

Query: 185 ENEAHVTQIVKDVWKMEFLGFFNFWNDYQETCSTQAFMMRDKKTDHDTIIVSFRGTEPFN 244
           EN   V  +V   WKM  + F + WNDYQ+  STQ F+  DK+ D + I++SFRGTEPF+
Sbjct: 187 ENAKVVENVVDLHWKMNLVEFLDCWNDYQKQMSTQVFVFTDKQKDANLIVISFRGTEPFD 246

Query: 245 ADDWSSDFDISWYEIQGIGKIHGGFMKALGL-----QKTFGW--------PKEIARQ--- 304
           ADDW +DFD SWYE+  +GK+H GF++A+GL       TF +         +E +++   
Sbjct: 247 ADDWGTDFDYSWYEVPNVGKLHMGFLEAMGLGNRDDTTTFHYNLFEQTSSEEENSKKNLL 306

Query: 305 DRRRPLAYYTLRERLRELLKENERTRFVVTGHSLGGALAILFPSILAFHEEKLLLERLEG 364
           D     AYY +R  L+ LL E+E  RFVVTGHSLGGALAILFP++L  +EE  +++RL G
Sbjct: 307 DMVERSAYYAVRVILKRLLSEHENARFVVTGHSLGGALAILFPTLLVLNEETEIMKRLLG 366

Query: 365 VYTFGQPRVGDGKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLPLDDKALMFKHFGPCIYF 424
           VYTFGQPR+G+ + G FM   L+Q   RY+R VY  D+VPRLP DDK  ++KHFG C+++
Sbjct: 367 VYTFGQPRIGNREVGLFMKAQLNQPVDRYFRVVYCNDIVPRLPYDDKTFLYKHFGLCLFY 426

Query: 425 DWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFTISWRRGKEYEERVMLRIMRLF 463
           D  Y     E+EP  N + +   +L  + A +E+ R  T+ +  G +Y+E     + RL 
Sbjct: 427 DSFYNETKAEDEPDPNPYGLRYKILGHVIAVWELVRGLTMGYTHGPDYKEGWFRILFRLM 486

[Close]

BLAST of Spg013448 vs. ExPASy Swiss-Prot
Match: Q5VKJ7 (Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 1.4e-84
Identity = 190/505 (37.62%), Postives = 274/505 (54.26%), Query Frame = 0


Query: 14  SNSYLLLKPEEVKLLDLLRLLFSSNL-KKRKFVDSSHTR------EYNFWHRFFIFLSIV 73
           S S+L++ P+E   LDL + +  S+L    KF  +S  R       Y+ W      +S+ 
Sbjct: 9   STSHLIVSPDEGTFLDLFKHIVLSDLGSGAKFFRASDQRVPATAAYYSRWP-----VSVF 68

Query: 74  VLKLLRFFATPLALFGFYLESCLNLLSLNHGFSGLLLNILRLRVKKPDSTSAEYLSMIGH 133
           + K+L+ F  P A+ G   +  LN    NHGF G+L NI  +R+K P    A+++S IG+
Sbjct: 69  ICKILQLFQMPAAMLGHLTDFLLNFYYQNHGFLGILRNIFLIRLKIPKRGEADFISTIGY 128

Query: 134 LDSRVML------------------DKNIKPG-DVNYFGA--------LCMMASKVVYEN 193
           LDSR+ L                  + ++K G +    GA        LC+MASK+ YEN
Sbjct: 129 LDSRMDLHGTPMVSHQADEVISNADNPSLKEGHNSKIKGALGNRSLMDLCIMASKLAYEN 188

Query: 194 EAHVTQIVKDVWKMEFLGFFNFWNDYQETCSTQAFMMRDKKTDHDTIIVSFRGTEPFNAD 253
              V ++V + WKM F+  +   N +Q+  +T AF+  DK  D + I++SFRGT PF+  
Sbjct: 189 TKVVERVVAEHWKMHFVADYGGMNYFQDARNTHAFIFCDKPKDANLIVISFRGTGPFSIP 248

Query: 254 DWSSDFDISWYEIQGIGKIHGGFMKALGL--------------QKTFGWPKEIARQDRRR 313
           +W +DFD S   +   G +H GF++A+GL               K+ G   E+ ++    
Sbjct: 249 NWCTDFDFSLVGLGDAGSVHVGFLEAMGLGHRNSISSFETSINTKSPGSITELRKESEMA 308

Query: 314 P--------LAYYTLRERLRELLKENERTRFVVTGHSLGGALAILFPSILAFHEEKLLLE 373
           P          Y+     L+ LLK+++  +FVVTGHSLGGALAILF  IL   +E  +L+
Sbjct: 309 PDHLVWAYDGVYFLAASTLKGLLKDHKNAKFVVTGHSLGGALAILFTCILEIQQETEVLD 368

Query: 374 RLEGVYTFGQPRVGDGKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLPLDDKALMFKHFGP 433
           RL  VYTFGQPR+G+   G FM   L+  + RY+R VY  DMVPR+P DD    F+HFG 
Sbjct: 369 RLLNVYTFGQPRIGNYNLGYFMQNRLNFPERRYFRVVYCNDMVPRVPFDDVFFTFEHFGT 428

Query: 434 CIYFDWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFTISWRRGKEYEERVMLRI 463
           CIY+D  +     +EEP +N F I  A+   I A +E+ RSF ++   G EY+E    R+
Sbjct: 429 CIYYDSRFFGYFTKEEPSRNPFGIENAISAHITAWWELWRSFILNHVYGAEYKETWESRM 488

[Close]

BLAST of Spg013448 vs. ExPASy Swiss-Prot
Match: P19515 (Lipase OS=Rhizomucor miehei OX=4839 PE=1 SV=2)

HSP 1 Score: 62.0 bits (149), Expect = 2.1e-08
Identity = 57/175 (32.57%), Postives = 79/175 (45.14%), Query Frame = 0


Query: 205 TIIVSFRGTEPFNADDWSSDF---DISWYEIQGIGKIHGGFMKALGLQKTFGWPKEIARQ 264
           TI + FRG+      +W +D     +S+  + G  K+H GF+ + G         E+  +
Sbjct: 168 TIYIVFRGSSSIR--NWIADLTFVPVSYPPVSGT-KVHKGFLDSYG---------EVQNE 227

Query: 265 DRRRPLAYYTLRERLRELLKENERTRFVVTGHSLGGALAILFPSILAFHEEKLLLERLEG 324
                     L   + +  K+    +  VTGHSLGGA A+L    L   EE L    L  
Sbjct: 228 ----------LVATVLDQFKQYPSYKVAVTGHSLGGATALLCALDLYQREEGLSSSNL-F 287

Query: 325 VYTFGQPRVGDGKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLPLDDKALMFKHFG 377
           +YT GQPRVGD  F  ++V +     I Y R V   D+VP LP    A  F H G
Sbjct: 288 LYTQGQPRVGDPAFANYVVST----GIPYRRTVNERDIVPHLP--PAAFGFLHAG 313

[Close]

BLAST of Spg013448 vs. ExPASy Swiss-Prot
Match: P61871 (Lipase OS=Rhizopus niveus OX=4844 PE=1 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 1.1e-07
Identity = 49/174 (28.16%), Postives = 82/174 (47.13%), Query Frame = 0


Query: 191 TQAFMMRDKKTDHDTIIVSFRGTEPFNADDWSSDFDISWYEIQGIGKIHGGFMKALGLQK 250
           T  +++R  K    TI + FRGT  F +      F+ S Y+     K+H GF+ +     
Sbjct: 186 TNGYVLRSDK--QKTIYLVFRGTNSFRSAITDIVFNFSDYKPVKGAKVHAGFLSSY---- 245

Query: 251 TFGWPKEIARQDRRRPLAYYTLRERLRELLKENERTRFVVTGHSLGGALAILFPSILAFH 310
                +++          Y+ +   ++E L  +   + +VTGHSLGGA A+L    L   
Sbjct: 246 -----EQVVND-------YFPV---VQEQLTAHPTYKVIVTGHSLGGAQALLAGMDLYQR 305

Query: 311 EEKLLLERLEGVYTFGQPRVGDGKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLP 365
           E +L  + L  ++T G PRVG+  F  ++  +     I + R V+  D+VP +P
Sbjct: 306 EPRLSPKNL-SIFTVGGPRVGNPTFAYYVEST----GIPFQRTVHKRDIVPHVP 333

[Close]

BLAST of Spg013448 vs. ExPASy TrEMBL
Match: A0A6J1HGB4 (uncharacterized protein LOC111463247 OS=Cucurbita moschata OX=3662 GN=LOC111463247 PE=4 SV=1)

HSP 1 Score: 857.8 bits (2215), Expect = 2.1e-245
Identity = 417/484 (86.16%), Postives = 442/484 (91.32%), Query Frame = 0


Query: 1   MAAASACNCNKGFSNSYLLLKPEEVKLLDLLRLLFSSNLKKRKFVDSSHTREYNFWHRFF 60
           MAA  A NCN GFS+SY+LLKPEE K LDL RLLFSSNLKKR+F+DSSH REYNFWHRFF
Sbjct: 1   MAARRAYNCNNGFSDSYMLLKPEEEKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFF 60

Query: 61  IFLSIVVLKLLRFFATPLALFGFYLESCLNLLSLNHGFSGLLLNILRLRVKKPDSTSAEY 120
           IFLSI+VLKLL F A PLALFGFYLES LN+LS NHGF G+LLNILRL++K PDS+SAEY
Sbjct: 61  IFLSILVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY 120

Query: 121 LSMIGHLDSRVMLDKNIKPGDVNYFGALCMMASKVVYENEAHVTQIVKDVWKMEFLGFFN 180
           LS+IGHLDSRV LD++IKPGDVNYFGALCMMASKVVYENEAHV Q V DVWKMEFL FFN
Sbjct: 121 LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLEFFN 180

Query: 181 FWNDYQETCSTQAFMMRDKKTDHDTIIVSFRGTEPFNADDWSSDFDISWYEIQGIGKIHG 240
           FWN+YQE CSTQAFMMRDK+ DHDTIIVSFRGTEPFNADDWSSDFDISWYEI+GIGKIHG
Sbjct: 181 FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG 240

Query: 241 GFMKALGLQKTFGWPKEIARQDRRRPLAYYTLRERLRELLKENERTRFVVTGHSLGGALA 300
           GFMKALGLQKT GWPKEI R+D+RRPLAYYTLRERLRELLKE+ERTRFVVTGHSLGGALA
Sbjct: 241 GFMKALGLQKTHGWPKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA 300

Query: 301 ILFPSILAFHEEKLLLERLEGVYTFGQPRVGDGKFGEFMVKSLSQYKIRYYRFVYGFDMV 360
           ILFPSILAFHEEKLLLERLEGVYTFGQPRVGD KFGEFMVKSLSQYKIRYYRFVYGFDMV
Sbjct: 301 ILFPSILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV 360

Query: 361 PRLPLDDKALMFKHFGPCIYFDWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT 420
           PRLPLD+  LM+KHFGPCIYF+WNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT
Sbjct: 361 PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT 420

Query: 421 ISWRRGKEYEERVMLRIMRLFGLLLPGIPAHCPQDYVNSTRLASTDIFFSPPKGRFHSEY 480
           I WRRGKEYEER+  RI+RL GLLLPGIPAHCPQDYVNSTRL ST        G  +SEY
Sbjct: 421 IGWRRGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGST--------GSLNSEY 476

Query: 481 DVKI 485
           DVKI
Sbjct: 481 DVKI 476

[Close]

BLAST of Spg013448 vs. ExPASy TrEMBL
Match: A0A1S3B1H5 (uncharacterized protein LOC107990286 OS=Cucumis melo OX=3656 GN=LOC107990286 PE=4 SV=1)

HSP 1 Score: 856.7 bits (2212), Expect = 4.8e-245
Identity = 417/479 (87.06%), Postives = 437/479 (91.23%), Query Frame = 0


Query: 8   NCNKGFSNSYLLLKPEEVKLLDLLRLLFSSNLKKRKFVDSSHTREYNFWHRFFIFLSIVV 67
           NCN GFS+SY+LL PEEVK LDLLRLLFSSNLKKR+FVDSSH RE+NFWHRFFIFLSIVV
Sbjct: 18  NCNGGFSSSYMLLNPEEVKFLDLLRLLFSSNLKKRRFVDSSHAREHNFWHRFFIFLSIVV 77

Query: 68  LKLLRFFATPLALFGFYLESCLNLLSLNHGFSGLLLNILRLRVKKPDSTSAEYLSMIGHL 127
           LKLLRFF  PLAL GF+LES LN LSLN GFSG+LLN  RLR+K PDS+SAEYLSMIGHL
Sbjct: 78  LKLLRFFDKPLALLGFFLESSLNFLSLNGGFSGILLNFFRLRLKIPDSSSAEYLSMIGHL 137

Query: 128 DSRVMLDKNIKPGDVNYFGALCMMASKVVYENEAHVTQIVKDVWKMEFLGFFNFWNDYQE 187
           DSRV LD +IKPGDVNYFGALCMMASK+ YENEAHV QIVKDVWKMEFLGFFNFWNDYQE
Sbjct: 138 DSRVTLDSSIKPGDVNYFGALCMMASKLSYENEAHVAQIVKDVWKMEFLGFFNFWNDYQE 197

Query: 188 TCSTQAFMMRDKKTDHDTIIVSFRGTEPFNADDWSSDFDISWYEIQGIGKIHGGFMKALG 247
            CSTQAFMMRDKK DHDTI+VSFRGTEPFNADDWSSDFDISWYEI+GIGKIHGGFMKALG
Sbjct: 198 KCSTQAFMMRDKKVDHDTIVVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKALG 257

Query: 248 LQKTFGWPKEIARQ--DRRRPLAYYTLRERLRELLKENERTRFVVTGHSLGGALAILFPS 307
           LQK  GWPKEI RQ  D RRPLAYYTLRE+L+EL+KENERTRFVVTGHSLGGALAILFPS
Sbjct: 258 LQKCIGWPKEIERQGHDERRPLAYYTLREKLKELVKENERTRFVVTGHSLGGALAILFPS 317

Query: 308 ILAFHEEKLLLERLEGVYTFGQPRVGDGKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLPL 367
           IL FHEEKLLLERLEGVYTFGQPRVGD  FGEFMVKSL+QYKIRYYRFVYGFDMVPRLPL
Sbjct: 318 ILVFHEEKLLLERLEGVYTFGQPRVGDRAFGEFMVKSLAQYKIRYYRFVYGFDMVPRLPL 377

Query: 368 DDKALMFKHFGPCIYFDWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFTISWRR 427
           DDKALMFKHFGPCIYFDWNYVAQILEEEPFKNYFSIVGA+LMRIHAC EIGRSFTISWRR
Sbjct: 378 DDKALMFKHFGPCIYFDWNYVAQILEEEPFKNYFSIVGAILMRIHACLEIGRSFTISWRR 437

Query: 428 GKEYEERVMLRIMRLFGLLLPGIPAHCPQDYVNSTRLASTDIFFSPPKGRFHSEYDVKI 485
           GKEYEE+V LRI+RLFGLLLPGIPAHCPQDYVNSTRL ST          F SEYDVK+
Sbjct: 438 GKEYEEKVFLRIVRLFGLLLPGIPAHCPQDYVNSTRLGST--------SHFLSEYDVKV 488

[Close]

BLAST of Spg013448 vs. ExPASy TrEMBL
Match: A0A6J1KN75 (uncharacterized protein LOC111497186 OS=Cucurbita maxima OX=3661 GN=LOC111497186 PE=4 SV=1)

HSP 1 Score: 855.9 bits (2210), Expect = 8.1e-245
Identity = 416/484 (85.95%), Postives = 443/484 (91.53%), Query Frame = 0


Query: 1   MAAASACNCNKGFSNSYLLLKPEEVKLLDLLRLLFSSNLKKRKFVDSSHTREYNFWHRFF 60
           MAA  A NC+ GFS+SY+LLKPEEVK LDL RLLFSSNLKKR+F+DSSH REYNFWHRFF
Sbjct: 1   MAARRAYNCSNGFSDSYMLLKPEEVKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFF 60

Query: 61  IFLSIVVLKLLRFFATPLALFGFYLESCLNLLSLNHGFSGLLLNILRLRVKKPDSTSAEY 120
           IFLSIVVLKLL F A PLALFGFYLES LN+LS NHGF G+LLNILRL++K PDS+SAEY
Sbjct: 61  IFLSIVVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY 120

Query: 121 LSMIGHLDSRVMLDKNIKPGDVNYFGALCMMASKVVYENEAHVTQIVKDVWKMEFLGFFN 180
           LS+IGHLDSRV LD++IKPGDVNYFGALCMMASKVVYENEAHV Q V DVWKMEFLGFFN
Sbjct: 121 LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLGFFN 180

Query: 181 FWNDYQETCSTQAFMMRDKKTDHDTIIVSFRGTEPFNADDWSSDFDISWYEIQGIGKIHG 240
           FWN+YQE CSTQAFMMRDK+ DHDTIIVSFRGTEPFNADDWSSDFDISWYEI+GIGKIHG
Sbjct: 181 FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG 240

Query: 241 GFMKALGLQKTFGWPKEIARQDRRRPLAYYTLRERLRELLKENERTRFVVTGHSLGGALA 300
           GFMKALGLQKT GW KEI R+D+RRPLAYYTLRERLRELLKE+ERTRFVVTGHSLGGALA
Sbjct: 241 GFMKALGLQKTHGWTKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA 300

Query: 301 ILFPSILAFHEEKLLLERLEGVYTFGQPRVGDGKFGEFMVKSLSQYKIRYYRFVYGFDMV 360
           ILFP+ILAFHEEKLLLERLEGVYTFGQPRVGD KFGEFMVKSLSQYKIRYYRFVYGFDMV
Sbjct: 301 ILFPTILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV 360

Query: 361 PRLPLDDKALMFKHFGPCIYFDWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT 420
           PRLPLD+  LM+KHFGPCIYF+WNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT
Sbjct: 361 PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT 420

Query: 421 ISWRRGKEYEERVMLRIMRLFGLLLPGIPAHCPQDYVNSTRLASTDIFFSPPKGRFHSEY 480
           I WRRGKEYEER+  RI+RLFGLLLPGIPAHCPQDYVN+TRL ST           +SEY
Sbjct: 421 IGWRRGKEYEERMCFRIVRLFGLLLPGIPAHCPQDYVNATRLGST--------VSLNSEY 476

Query: 481 DVKI 485
           DVKI
Sbjct: 481 DVKI 476

[Close]

BLAST of Spg013448 vs. ExPASy TrEMBL
Match: A0A5D3CNF4 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G004760 PE=4 SV=1)

HSP 1 Score: 848.2 bits (2190), Expect = 1.7e-242
Identity = 411/472 (87.08%), Postives = 433/472 (91.74%), Query Frame = 0


Query: 8   NCNKGFSNSYLLLKPEEVKLLDLLRLLFSSNLKKRKFVDSSHTREYNFWHRFFIFLSIVV 67
           NCN GFS+SY+LL PEEVK LDLLRLLFSSNLKKR+FVDSSH RE+NFWHRFFIFLSIVV
Sbjct: 15  NCNGGFSSSYMLLNPEEVKFLDLLRLLFSSNLKKRRFVDSSHAREHNFWHRFFIFLSIVV 74

Query: 68  LKLLRFFATPLALFGFYLESCLNLLSLNHGFSGLLLNILRLRVKKPDSTSAEYLSMIGHL 127
           LKLLRFF  PLAL GF+LES LN LSLN GFSG+LLN  RLR+K PDS+SAEYLSMIGHL
Sbjct: 75  LKLLRFFDKPLALLGFFLESSLNFLSLNGGFSGILLNFFRLRLKIPDSSSAEYLSMIGHL 134

Query: 128 DSRVMLDKNIKPGDVNYFGALCMMASKVVYENEAHVTQIVKDVWKMEFLGFFNFWNDYQE 187
           DSRV LD++IKPGDVNYFGALCMMASK+ YENEAHV QIVKDVWKMEFLGFFNFWNDYQE
Sbjct: 135 DSRVTLDRSIKPGDVNYFGALCMMASKLSYENEAHVAQIVKDVWKMEFLGFFNFWNDYQE 194

Query: 188 TCSTQAFMMRDKKTDHDTIIVSFRGTEPFNADDWSSDFDISWYEIQGIGKIHGGFMKALG 247
            CSTQAFMMRDKK DHDTI+VSFRGTEPFNADDWSSDFDISWYEI+GIGKIHGGFMKALG
Sbjct: 195 KCSTQAFMMRDKKVDHDTIVVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKALG 254

Query: 248 LQKTFGWPKEIARQ--DRRRPLAYYTLRERLRELLKENERTRFVVTGHSLGGALAILFPS 307
           LQK  GWPKEI RQ  D RRPLAYYTLRE+L+EL+KENERTRFVVTGHSLGGALAILFPS
Sbjct: 255 LQKCIGWPKEIERQGHDERRPLAYYTLREKLKELVKENERTRFVVTGHSLGGALAILFPS 314

Query: 308 ILAFHEEKLLLERLEGVYTFGQPRVGDGKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLPL 367
           IL FHEEKLLLERLEGVYTFGQPRVGD  FGEFMVKSL+QYKIRYYRFVYGFDMVPRLPL
Sbjct: 315 ILVFHEEKLLLERLEGVYTFGQPRVGDRAFGEFMVKSLAQYKIRYYRFVYGFDMVPRLPL 374

Query: 368 DDKALMFKHFGPCIYFDWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFTISWRR 427
           DDKALMFKHFGPCIYFDWNYVAQILEEEPFKNYFSIVGA+LMRIHAC EIGRSFTISWRR
Sbjct: 375 DDKALMFKHFGPCIYFDWNYVAQILEEEPFKNYFSIVGAILMRIHACLEIGRSFTISWRR 434

Query: 428 GKEYEERVMLRIMRLFGLLLPGIPAHCPQDYVNSTRLASTDIFFSPPKGRFH 478
           GKEYEE+V LRI+RLFGLLLPGI AHCPQDYVNSTRL ST  F    K + +
Sbjct: 435 GKEYEEKVFLRIVRLFGLLLPGILAHCPQDYVNSTRLGSTSHFLKLRKEKLY 486

[Close]

BLAST of Spg013448 vs. ExPASy TrEMBL
Match: A0A0A0KFS7 (Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G490940 PE=4 SV=1)

HSP 1 Score: 845.5 bits (2183), Expect = 1.1e-241
Identity = 409/478 (85.56%), Postives = 436/478 (91.21%), Query Frame = 0


Query: 8   NCNKGFSNSYLLLKPEEVKLLDLLRLLFSSNLKKRKFVDSSHTREYNFWHRFFIFLSIVV 67
           NCN GFSNSY+LL PEEVK LDL RLLFSSNLKKR+FVDSSH RE+NFWHRFFIFLSIVV
Sbjct: 17  NCNGGFSNSYMLLNPEEVKFLDLFRLLFSSNLKKRRFVDSSHAREHNFWHRFFIFLSIVV 76

Query: 68  LKLLRFFATPLALFGFYLESCLNLLSLNHGFSGLLLNILRLRVKKPDSTSAEYLSMIGHL 127
           LKLLRFF  PLAL GF+LES LN LSLN GFSG+LLN  RLR+K P+++SAEYLSMIGHL
Sbjct: 77  LKLLRFFDKPLALLGFFLESSLNFLSLNGGFSGILLNFFRLRLKIPNTSSAEYLSMIGHL 136

Query: 128 DSRVMLDKNIKPGDVNYFGALCMMASKVVYENEAHVTQIVKDVWKMEFLGFFNFWNDYQE 187
           DSRV LD++IKPGDVNYFGALCMMASK+ YENEAHV QIVKDVWKMEFLGFFNFWNDYQE
Sbjct: 137 DSRVTLDRSIKPGDVNYFGALCMMASKLSYENEAHVAQIVKDVWKMEFLGFFNFWNDYQE 196

Query: 188 TCSTQAFMMRDKKTDHDTIIVSFRGTEPFNADDWSSDFDISWYEIQGIGKIHGGFMKALG 247
            CSTQAFMMRD+K +HDTIIVSFRGTEPFNADDWSSDFDISWYEI+GIGKIHGGFMKALG
Sbjct: 197 KCSTQAFMMRDRKDNHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKALG 256

Query: 248 LQKTFGWPKEIARQ-DRRRPLAYYTLRERLRELLKENERTRFVVTGHSLGGALAILFPSI 307
           LQK  GWPKE+ R+   R PLAYYTLRE+L+EL+KENERTRFVVTGHSLGGALAILFPSI
Sbjct: 257 LQKCVGWPKEMERKGHERHPLAYYTLREKLKELMKENERTRFVVTGHSLGGALAILFPSI 316

Query: 308 LAFHEEKLLLERLEGVYTFGQPRVGDGKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLPLD 367
           L FHEEKLLLERLEGVYTFGQPRVGD  FGEFMVKSL++YKIRYYRFVYGFDMVPRLPLD
Sbjct: 317 LVFHEEKLLLERLEGVYTFGQPRVGDRTFGEFMVKSLAEYKIRYYRFVYGFDMVPRLPLD 376

Query: 368 DKALMFKHFGPCIYFDWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFTISWRRG 427
           DKALMFKHFGPCIYFDWNYVAQILEEEPFKNYFSIVGA+LMRIHAC EIGRSFTISWRRG
Sbjct: 377 DKALMFKHFGPCIYFDWNYVAQILEEEPFKNYFSIVGAILMRIHACLEIGRSFTISWRRG 436

Query: 428 KEYEERVMLRIMRLFGLLLPGIPAHCPQDYVNSTRLASTDIFFSPPKGRFHSEYDVKI 485
           KEYEE+V LRI+RLFGLLLPGIPAHCPQDYVNSTRL ST          FH+EYDVK+
Sbjct: 437 KEYEEKVFLRIVRLFGLLLPGIPAHCPQDYVNSTRLGSTT--------PFHTEYDVKV 486

[Close]

BLAST of Spg013448 vs. TAIR 10
Match: AT5G67050.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 491.1 bits (1263), Expect = 1.0e-138
Identity = 245/460 (53.26%), Postives = 326/460 (70.87%), Query Frame = 0


Query: 17  YLLLKPEEVKLLDLLRLLFSSNLKKRKFVDSSHTREYNFWHRFFIFLSIVVLKLLRFFAT 76
           YL+L+PEE++  +L+RLLFS +++K + VDSS T E++F HR+ IF+S+V+LKLLRFF+ 
Sbjct: 13  YLILRPEELRPWELVRLLFSGDIEKPRSVDSSETEEHSFRHRWLIFVSLVLLKLLRFFSK 72

Query: 77  PLALFGFYLESCLNLLSLNHGFSGLLLNILRLRVKKPDSTSAEYLSMIGHLDSRVMLDKN 136
            LAL G  LE  LN LS N+ FSGL    LR  V  P  TS  Y S IGHLD+RV LD  
Sbjct: 73  LLALVGSALEFSLNFLS-NNSFSGL---FLRGEVVMPQRTSENYQSFIGHLDTRVSLDMT 132

Query: 137 IKPGD-VNYFGALCMMASKVVYENEAHVTQIVKDVWKMEFLGFFNFWNDYQETCSTQAFM 196
           +   D   Y+ AL +MASK+ YEN A +  +V++ W M++LG  ++WN+YQE  +TQAF+
Sbjct: 133 LNREDGEKYYAALSIMASKIAYENAARIKHVVENHWNMKYLGLVDYWNEYQEKETTQAFI 192

Query: 197 MRDKKT------DHDTIIVSFRGTEPFNADDWSSDFDISWYEIQGIGKIHGGFMKALGLQ 256
           M   +T         T++V+FRGTE FN++DW SDFDI+W+E+  IG IHGGFMKALGLQ
Sbjct: 193 MSTDETTTRSNGQETTVVVAFRGTELFNSEDWCSDFDITWFELPNIGNIHGGFMKALGLQ 252

Query: 257 KTFGWPKE-IARQDRRRPLAYYTLRERLRELLKENERTRFVVTGHSLGGALAILFPSILA 316
               WPKE ++  DR+ PLAYY++R+ L+ L+ +N+ T+FV+TGHSLGGALAILF ++L 
Sbjct: 253 NNCSWPKEPLSNPDRKSPLAYYSIRDSLKTLIAQNKNTKFVLTGHSLGGALAILFTAVLV 312

Query: 317 FHEEKLLLERLEGVYTFGQPRVGDGKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLPLDDK 376
            H E  LLER++GVYT+GQPRVGD KFGEFM K L +Y I+YYRFVY  D+VPRLP DDK
Sbjct: 313 IHHETELLERIQGVYTYGQPRVGDSKFGEFMEKKLEKYNIKYYRFVYNNDIVPRLPYDDK 372

Query: 377 ALMFKHFGPCIYFDWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFTISWRRGKE 436
            LMFKHFG CIY+D NY A+++ E+  +N+F + G + M   A  E  RSFTI   +G E
Sbjct: 373 DLMFKHFGTCIYYDQNYQAKVMREQSDENFFLLRGIIKMMWSAILEFIRSFTIVAEKGSE 432

Query: 437 YEERVMLRIMRLFGLLLPGIPAHCPQDYVNSTRLASTDIF 469
           Y E  +L+  R  G+++PG+  H PQDYVN+TRL    +F
Sbjct: 433 YSEGWLLKGGRALGIIVPGVSNHTPQDYVNATRLTLPCVF 468

[Close]

BLAST of Spg013448 vs. TAIR 10
Match: AT5G42930.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 451.4 bits (1160), Expect = 8.9e-127
Identity = 226/456 (49.56%), Postives = 312/456 (68.42%), Query Frame = 0


Query: 14  SNSYLLLKPEEVKLLDLLRLLFSSNLKKRKFVDSSHTREYN----FWHRFFIFLSIVVLK 73
           + +Y +L P E  + DL+ LLFSS+L  RKF+ SS  R  +    F  R+ IF+SIV+ K
Sbjct: 4   TKNYFVLDPREATVSDLIHLLFSSDLGDRKFIHSSEERIEDDLSRFRRRWIIFVSIVIQK 63

Query: 74  LLRFFATPLALFGFYLESCLNLLSLNHGFSGLLLNILRLRVKKPDSTSAEYLSMIGHLDS 133
           L+  F  PL   GF L   LNLLS N GF  +L N+ +  +  P+  SA + S+ G+LD 
Sbjct: 64  LIILFKKPLYFVGFSLACWLNLLSSNGGFLMILPNLFKGNIIWPEKASATFASLNGNLDR 123

Query: 134 RVMLDKNIKPGDVNYFGALCMMASKVVYENEAHVTQIVKDVWKMEFLGFFNFWNDYQETC 193
           RV L+  ++ G   Y   L +MASK+ YEN   V+ ++ + WKM+ LGF++ WN YQ+  
Sbjct: 124 RVELNPKVERGSKRYKAMLSIMASKLSYENINFVSSVLHNHWKMDLLGFYSCWNGYQKQR 183

Query: 194 STQAFMMRDKKTDHDTIIVSFRGTEPFNADDWSSDFDISWYEIQGIGKIHGGFMKALGLQ 253
           ST+  +++D  TD + IIVSFRGT+PF+ADDW +D D+SWYE++ +GKIHGGFMKALGLQ
Sbjct: 184 STEVIVIKDTSTDPNLIIVSFRGTDPFDADDWCTDLDLSWYEVKNVGKIHGGFMKALGLQ 243

Query: 254 KTFGWPKEI---ARQDRRRPLAYYTLRERLRELLKENERTRFVVTGHSLGGALAILFPSI 313
           K  GWPKE+     Q+     AYYT+R  L+E+L +N  ++F++TGHSLGGALAILF ++
Sbjct: 244 KE-GWPKEVNLDETQNATTLYAYYTVRRHLKEILDQNPTSKFILTGHSLGGALAILFTAV 303

Query: 314 LAFHEEKLLLERLEGVYTFGQPRVGDGKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLPLD 373
           L  H+E+ +LERLEGVYTFGQPRVGD +FG FM  SL ++ ++Y R+VY  DMVPRLP D
Sbjct: 304 LVMHDEEQMLERLEGVYTFGQPRVGDEEFGNFMKDSLKKFDVKYERYVYCNDMVPRLPFD 363

Query: 374 DKALMFKHFGPCIYFDWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFTISWRRG 433
           DK LMFKHFG C+Y+D  Y  ++ EEEP KNYF++V  +   ++A +E+ RSF + + +G
Sbjct: 364 DKTLMFKHFGACLYYDSFYKGKVEEEEPNKNYFNLVWVLPKIMNALWELIRSFVMPYWKG 423

Query: 434 KEYEERVMLRIMRLFGLLLPGIPAHCPQDYVNSTRL 463
            E+ E   LR  R+  LL+PG+PAH P +Y+N T L
Sbjct: 424 GEFREGWFLRCFRVVALLIPGLPAHFPNEYINVTLL 458

[Close]

BLAST of Spg013448 vs. TAIR 10
Match: AT1G56630.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 438.0 bits (1125), Expect = 1.0e-122
Identity = 227/458 (49.56%), Postives = 311/458 (67.90%), Query Frame = 0


Query: 16  SYLLLKPEEVKLLDLLRLLFSSNLKKRKFVDSS----HTREYNFWHRFFIFLSIVVLKLL 75
           +Y +L P E  + DL+RLLF S+L+ RKFVD+S      R   F  R+ IF+SIVV KLL
Sbjct: 6   NYFVLDPREATVTDLMRLLFYSDLENRKFVDTSVENLENRLCEFRGRWIIFVSIVVQKLL 65

Query: 76  RFFATPLALFGFYLESCLNLLSLNHGFSGLLLNILRLRVKKPDSTSAEYLSMIGHLDSRV 135
                PL+  GF L   LNL S N GF  + LN+++ R   P+ TSA + S+ G+LD +V
Sbjct: 66  IILRKPLSFLGFALGFWLNLPSSNGGFFKIFLNLVKGRFIWPEKTSATFASINGNLDQKV 125

Query: 136 M--LDKNIKPGDVNYFGALCMMASKVVYENEAHVTQIVKDVWKMEFLGFFNFWNDYQETC 195
              L ++IK GD  Y   L +MASK+ YENE  +  +++D W+M+ LGF++  ND+ +T 
Sbjct: 126 ELGLGRSIKIGDERYKPLLSIMASKLAYENEHFIRSVLQDHWQMDLLGFYSCPNDFDQTR 185

Query: 196 STQAFMMRDKKTDHDTIIVSFRGTEPFNADDWSSDFDISWYEIQGIGKIHGGFMKALGLQ 255
           ST+  ++RD K + + I+VSFRGT+PFNADDW +D D+SW+ +  +GKIHGGFMKALGL 
Sbjct: 186 STEVIVIRDTKDNPNLIVVSFRGTDPFNADDWCTDLDLSWHNVMNVGKIHGGFMKALGLP 245

Query: 256 KTFGWPKEI---ARQDRRRPLAYYTLRERLRELLKENERTRFVVTGHSLGGALAILFPSI 315
           K  GW +EI     Q++   LAYYT+  +L+E+ ++N  ++F+++GHSLGGALAILF ++
Sbjct: 246 KE-GWYEEINSDQTQNKTSQLAYYTILRQLKEVFEQNPTSKFILSGHSLGGALAILFTAV 305

Query: 316 LAFHEEKLLLERLEGVYTFGQPRVGDGKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLPLD 375
           L  H+EK +LERLEGVYTFGQPRVGD  FG +M   L ++ ++Y R+VY  DMVPRLP D
Sbjct: 306 LIMHDEKEMLERLEGVYTFGQPRVGDEDFGIYMKDKLKEFDVKYKRYVYCNDMVPRLPFD 365

Query: 376 DKALMFKHFGPCIYFDWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFTISWRRG 435
           DK LMFKHFG C+Y D  Y  ++ EEEP KNYF+I   +   I+A +E+ RSF IS  +G
Sbjct: 366 DKTLMFKHFGGCLYCDSFYKGKVEEEEPNKNYFNIFWVIPKIINAVWELIRSFIISCWQG 425

Query: 436 KEYEERVMLRIMRLFGLLLPGIPAHCPQDYVNSTRLAS 465
           +EY E  +L   RL  LL+PG+PAH P +YVN   L +
Sbjct: 426 REYREGWLLTCFRLVALLIPGLPAHFPNEYVNVALLGN 462

[Close]

BLAST of Spg013448 vs. TAIR 10
Match: AT1G45201.1 (triacylglycerol lipase-like 1 )

HSP 1 Score: 419.5 bits (1077), Expect = 3.8e-117
Identity = 213/458 (46.51%), Postives = 299/458 (65.28%), Query Frame = 0


Query: 10  NKGFSNSYLLLKPEEVKLLDLLRLLFSSNLKKRKFVDSSHTR----EYNFWHRFFIFLSI 69
           N  F NSY L+ P +   LDLL LLFSSNL   +F+DS          +F  R+ + L+I
Sbjct: 5   NMKFCNSYFLVDPTKASFLDLLLLLFSSNLTSARFIDSPPDTLKGFRRSFASRWILALAI 64

Query: 70  VVLKLLRFFATPLALFGFYLESCLNLLSLNHGFSGLLLNILRLRVKKPDSTSAEYLSMIG 129
            + K+L   + P A  G  L   LNLL+ N GF  L+LN++  ++ KPD +SA Y S IG
Sbjct: 65  FLQKVLMLLSKPFAFIGQKLTYWLNLLTANGGFFNLILNLMSGKLVKPDKSSATYTSFIG 124

Query: 130 HLDSRVMLDKNIKPGDVNYFGALCMMASKVVYENEAHVTQIVKDVWKMEFLGFFNFWNDY 189
             D R+ LD+ I  G + Y   L +MASK+ YE++ ++T +VK+ WKM+ +G ++F+N +
Sbjct: 125 CSDRRIELDEKINVGSIEYKSMLSIMASKISYESKPYITSVVKNTWKMDLVGNYDFYNAF 184

Query: 190 QETCSTQAFMMRDKKTDHDTIIVSFRGTEPFNADDWSSDFDISWYEIQGIGKIHGGFMKA 249
           QE+  TQAF+ +   T+ D I+VSFRGTEPF A DW +D D+SWYE++ +GK+H GF +A
Sbjct: 185 QESKLTQAFVFKTSSTNPDLIVVSFRGTEPFEAADWCTDLDLSWYEMKNVGKVHAGFSRA 244

Query: 250 LGLQKTFGWPKEIARQDRRRPLAYYTLRERLRELLKENERTRFVVTGHSLGGALAILFPS 309
           LGLQK  GWPKE          AYYT+R+ LR+ L  N+  ++++TGHSLGGALA LFP+
Sbjct: 245 LGLQKD-GWPKE--NISLLHQYAYYTIRQMLRDKLGRNKNLKYILTGHSLGGALAALFPA 304

Query: 310 ILAFHEEKLLLERLEGVYTFGQPRVGDGKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLPL 369
           ILA H E  LL++LEG+YTFGQPRVGD  FGEFM   + ++ I Y RFVY  D+VPR+P 
Sbjct: 305 ILAIHGEDELLDKLEGIYTFGQPRVGDEDFGEFMKGVVKKHGIEYERFVYNNDVVPRVPF 364

Query: 370 DDKALM-FKHFGPCIYFDWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFTISWR 429
           DDK L  +KH+GPC  F+  Y  ++ E+ P  NYF+++  +   +   +E  RSF + + 
Sbjct: 365 DDKYLFSYKHYGPCNSFNSLYKGKVREDAPNANYFNLLWLIPQLLTGLWEFIRSFILQFW 424

Query: 430 RGKEYEERVMLRIMRLFGLLLPGIPAHCPQDYVNSTRL 463
           +G EY+E  ++R +R+ G++ PG   H P DYVNSTRL
Sbjct: 425 KGDEYKENWLMRFVRVVGIVFPGGSNHFPFDYVNSTRL 459

[Close]

BLAST of Spg013448 vs. TAIR 10
Match: AT3G14360.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 365.5 bits (937), Expect = 6.4e-101
Identity = 205/501 (40.92%), Postives = 294/501 (58.68%), Query Frame = 0


Query: 5   SACNCNKGFSNSYLLLKPEEVKLLDLLRL-LFSSNLKKRKFVDSSHTREYNFW------- 64
           S    N G  ++YL+++P     +DL R  +      K KF++    RE++         
Sbjct: 7   SGSGSNPGQVSNYLIVRPHRGGYIDLFRYGVRDDQTSKAKFLEMPDNREWSTITIDEEAE 66

Query: 65  -HRFFIFLSIVVLKLLRFFATPLALFGFYLESCLNLLSLNHGFSGLLLNILRLRVKKPDS 124
            HR+ I +SI+V K++R   TP+   GF ++  LNL S N GF GLLL +++ +V  P+ 
Sbjct: 67  DHRWVIVVSILVRKIIRLLRTPMEFTGFVVDFFLNLFSANGGFFGLLLRLIQAKVVIPER 126

Query: 125 TSAEYLSMIGHLDSRVMLDK------------NIKPGDVNY-FGA-----LCMMASKVVY 184
            S  ++S IG LD R+ L K            ++  G V    G+     LC+MASK+ Y
Sbjct: 127 GSVTFVSTIGQLDGRISLYKEWNFVEHLEGIDSVDSGRVKIELGSRGLMDLCVMASKLAY 186

Query: 185 ENEAHVTQIVKDVWKMEFLGFFNFWNDYQETCSTQAFMMRDKKTDHDTIIVSFRGTEPFN 244
           EN   V  +V   WKM  + F + WNDYQ+  STQ F+  DK+ D + I++SFRGTEPF+
Sbjct: 187 ENAKVVENVVDLHWKMNLVEFLDCWNDYQKQMSTQVFVFTDKQKDANLIVISFRGTEPFD 246

Query: 245 ADDWSSDFDISWYEIQGIGKIHGGFMKALGL-----QKTFGW--------PKEIARQ--- 304
           ADDW +DFD SWYE+  +GK+H GF++A+GL       TF +         +E +++   
Sbjct: 247 ADDWGTDFDYSWYEVPNVGKLHMGFLEAMGLGNRDDTTTFHYNLFEQTSSEEENSKKNLL 306

Query: 305 DRRRPLAYYTLRERLRELLKENERTRFVVTGHSLGGALAILFPSILAFHEEKLLLERLEG 364
           D     AYY +R  L+ LL E+E  RFVVTGHSLGGALAILFP++L  +EE  +++RL G
Sbjct: 307 DMVERSAYYAVRVILKRLLSEHENARFVVTGHSLGGALAILFPTLLVLNEETEIMKRLLG 366

Query: 365 VYTFGQPRVGDGKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLPLDDKALMFKHFGPCIYF 424
           VYTFGQPR+G+ + G FM   L+Q   RY+R VY  D+VPRLP DDK  ++KHFG C+++
Sbjct: 367 VYTFGQPRIGNREVGLFMKAQLNQPVDRYFRVVYCNDIVPRLPYDDKTFLYKHFGLCLFY 426

Query: 425 DWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFTISWRRGKEYEERVMLRIMRLF 463
           D  Y     E+EP  N + +   +L  + A +E+ R  T+ +  G +Y+E     + RL 
Sbjct: 427 DSFYNETKAEDEPDPNPYGLRYKILGHVIAVWELVRGLTMGYTHGPDYKEGWFRILFRLM 486

The following BLAST results are available for this feature:

BLAST of Spg013448 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6595033.1	4.7e-247	86.78	Lipase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027056.1 Lipase, pa...	[more]
XP_023518931.1	2.3e-246	86.57	uncharacterized protein LOC111782304 [Cucurbita pepo subsp. pepo]	[more]
XP_022962880.1	4.4e-245	86.16	uncharacterized protein LOC111463247 [Cucurbita moschata]	[more]
XP_008440283.1	9.9e-245	87.06	PREDICTED: uncharacterized protein LOC107990286 [Cucumis melo]	[more]
XP_023003657.1	1.7e-244	85.95	uncharacterized protein LOC111497186 [Cucurbita maxima]	[more]

BLAST of Spg013448 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A1S3ZP85	1.8e-100	43.61	Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1	[more]
F4JFU8	9.0e-100	40.92	Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1	[more]
Q5VKJ7	1.4e-84	37.62	Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1	[more]
P19515	2.1e-08	32.57	Lipase OS=Rhizomucor miehei OX=4839 PE=1 SV=2	[more]
P61871	1.1e-07	28.16	Lipase OS=Rhizopus niveus OX=4844 PE=1 SV=1	[more]

BLAST of Spg013448 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HGB4	2.1e-245	86.16	uncharacterized protein LOC111463247 OS=Cucurbita moschata OX=3662 GN=LOC1114632...	[more]
A0A1S3B1H5	4.8e-245	87.06	uncharacterized protein LOC107990286 OS=Cucumis melo OX=3656 GN=LOC107990286 PE=...	[more]
A0A6J1KN75	8.1e-245	85.95	uncharacterized protein LOC111497186 OS=Cucurbita maxima OX=3661 GN=LOC111497186...	[more]
A0A5D3CNF4	1.7e-242	87.08	Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A0A0KFS7	1.1e-241	85.56	Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G490940 PE...	[more]

BLAST of Spg013448 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G67050.1	1.0e-138	53.26	alpha/beta-Hydrolases superfamily protein	[more]
AT5G42930.1	8.9e-127	49.56	alpha/beta-Hydrolases superfamily protein	[more]
AT1G56630.1	1.0e-122	49.56	alpha/beta-Hydrolases superfamily protein	[more]
AT1G45201.1	3.8e-117	46.51	triacylglycerol lipase-like 1	[more]
AT3G14360.1	6.4e-101	40.92	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002921	Fungal lipase-like domain	PFAM	PF01764	Lipase_3	coord: 207..367 e-value: 4.3E-38 score: 130.4
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 121..406 e-value: 2.5E-47 score: 163.9
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 148..405
IPR044819	Triacylglycerol lipase OBL1-like	PANTHER	PTHR46086	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 9..469
None	No IPR available	PANTHER	PTHR46086:SF17	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 9..469
None	No IPR available	CDD	cd00519	Lipase_3	coord: 148..382 e-value: 1.27872E-47 score: 162.26

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg013448.1	Spg013448.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004806	triglyceride lipase activity