Spg013422 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg013422
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionPhenylalanine ammonia-lyase
Locationscaffold1: 13145330 .. 13147480 (+)
RNA-Seq ExpressionSpg013422
SyntenySpg013422
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCACCTGCTCACTTCCAACAACTTGCCTGCCAGAATGGTTCCATTGAGAGCTTCTGCATCAGCCAAAATGATCCACTCAACTGGGGTGTGGCCGCCGAGTCCCTCAAGGGAAGCCATCTCGATGAGGTGAAGCGCATGGTAGAGGAGTACCGGAGGCCACTCGTCAAGCTTGGAGGTGAGACCCTCACCATTTCCCAGGTGGCTGCCATTGCTACTAGGGACTCTGATGTTACTGTTGAGCTTTCTGAATCTGCTAGAGCTGGTGTTAAAGCTAGTAGCGATTGGGTTATGGAGAGCATGAACAAAGGCACCGACAGTTATGGTGTTACTACTGGATTTGGTGCTACGTCTCATCGAAGGACCAAACAGGGTGGGGCTCTTCAGAAGGAGCTCATTCGATTCTTAAATGCTGGAATTTTTGGGAGCGGGGCTGAGTCGAATCATACTTTGCCTCACTCGGCTACGAGGGCGGCCATGTTGGTGAGAATCAATACTCTTCTTCAAGGCTACTCGGGTATTAGATTTGAGATTTTGGAAGCCATAACCAAACTCTTGAACCATAACATCACTCCATGCTTGCCTCTTCGTGGAACCATTACTGCTTCTGGAGACCTTGTTCCTCTTTCTTATATTGCCGGATTGCTCACTGGCCGACCAAACTCTAAGGCCATCGGGCCGAATGGAGAAAGTATTGATGCCAAGGAGGCTTTCCAACAAGCTGGTATTCCTTCTGGGTTCTTTGAGTTGCAGCCTAAGGAAGGTCTTGCACTTGTGAATGGCACCGCTGTTGGTTCTGGATTGGCTTCTATTGTCCTTTTTGAAGCAAATATTTTAGCCGTTTTGTCGGAAGTTCTTTCTGCCATTTTTGCTGAGGTCATGCAAGGAAAGCCTGAATTCACCGATCATTTGACTCATAAGTTGAAGCACCACCCTGGTCAGATTGAAGCGGCAGCAATCATGGAGCACATTTTGGATGGAAGTTCCTACATGAAAACAGCCAAGAAATTGCATGAAATTGATCCTCTCCAAAAGCCCAAACAAGATCGTTATGCTCTCAGGACTTCACCCCAATGGCTTGGTCCCTTGATTGAAGTGATTCGATTCTCCACCAAGTCCATCGAGCGAGAGATTAATTCAGTCAACGACAACCCATTGATTGATGTTTCAAGAAACAAAGCACTACATGGAGGCAACTTCCAAGGAACACCGATTGGAGTTTCTATGGACAACACTCGGTTGGCAATTGCTTCAATTGGAAAGCTCATGTTTGCCCAATTCTCAGAACTTGTCAACGATTTCTACAACAATGGTTTGCCTTCTAATCTTTCTGCAAGTAGAAATCCGAGCTTGGATTATGGGTTTAAAGGTGCTGAAATTGCCATGGCTTCATACTGCTCTGAGCTTCAATATCTTGCAAATCCAGTGACAAGTCATGTACAAAGTGCCGAGCAACACAATCAAGATGTTAACTCTCTTGGTTTAATTTCTTCAAGGAAAACTGTCGAAGCCATCGATATTCTGAAACTCATGTCATCTACATTTTTGGTTGCACTCTGTCAAGCAATTGACTTGAGGCATTTGGAGGAAAACTTGAAGAGCACTGTGAAAAGCACGGTTAGCCAAATAGCCAAGAAAGTGTTGACCACATCCTCTAATGGTGCACTTCACCCATCAAGATTTTGTGAGAAAGATCTACTTAAAGTTGTTGACAGGGAGTACACTTTTGCTTATATCGATGATCCATGCAGTGCTACTTACCCATTGAGTCAAAAGTTGAGACAAGTTCTTGTTGAACATGCACTTGCCAACGGTGAAGGTGAAAAGAACACAGACACCTCAATCTTCCAAAAGATTATTGCTTTCGAGGAGGAACTAAAAGCAGTATTACCAAAGGAGGTTGAAAATGCAAGGTTGTCATATGAGAGTGGAAATTCTAAAATTGAAAATCAGATCAAGGATTGCAGATCTTATCCATTGTATAGATTTGTGAGAGAAGAGTTGGGAACAAAATTGCTGACCGGAGAAAAAGTGATATCTCCAGGAGAAGAGTGCGAGAAGGTGTTCACAGCTTTGTGCCAAGGCAAGATGATTGACTCCATCTTAGAGTGCTTGAAGGAATGGAATGGTGCTCCAATTCCTATCTGCTAA

mRNA sequence

ATGGCACCTGCTCACTTCCAACAACTTGCCTGCCAGAATGGTTCCATTGAGAGCTTCTGCATCAGCCAAAATGATCCACTCAACTGGGGTGTGGCCGCCGAGTCCCTCAAGGGAAGCCATCTCGATGAGGTGAAGCGCATGGTAGAGGAGTACCGGAGGCCACTCGTCAAGCTTGGAGGTGAGACCCTCACCATTTCCCAGGTGGCTGCCATTGCTACTAGGGACTCTGATGTTACTGTTGAGCTTTCTGAATCTGCTAGAGCTGGTGTTAAAGCTAGTAGCGATTGGGTTATGGAGAGCATGAACAAAGGCACCGACAGTTATGGTGTTACTACTGGATTTGGTGCTACGTCTCATCGAAGGACCAAACAGGGTGGGGCTCTTCAGAAGGAGCTCATTCGATTCTTAAATGCTGGAATTTTTGGGAGCGGGGCTGAGTCGAATCATACTTTGCCTCACTCGGCTACGAGGGCGGCCATGTTGGTGAGAATCAATACTCTTCTTCAAGGCTACTCGGGTATTAGATTTGAGATTTTGGAAGCCATAACCAAACTCTTGAACCATAACATCACTCCATGCTTGCCTCTTCGTGGAACCATTACTGCTTCTGGAGACCTTGTTCCTCTTTCTTATATTGCCGGATTGCTCACTGGCCGACCAAACTCTAAGGCCATCGGGCCGAATGGAGAAAGTATTGATGCCAAGGAGGCTTTCCAACAAGCTGGTATTCCTTCTGGGTTCTTTGAGTTGCAGCCTAAGGAAGGTCTTGCACTTGTGAATGGCACCGCTGTTGGTTCTGGATTGGCTTCTATTGTCCTTTTTGAAGCAAATATTTTAGCCGTTTTGTCGGAAGTTCTTTCTGCCATTTTTGCTGAGGTCATGCAAGGAAAGCCTGAATTCACCGATCATTTGACTCATAAGTTGAAGCACCACCCTGGTCAGATTGAAGCGGCAGCAATCATGGAGCACATTTTGGATGGAAGTTCCTACATGAAAACAGCCAAGAAATTGCATGAAATTGATCCTCTCCAAAAGCCCAAACAAGATCGTTATGCTCTCAGGACTTCACCCCAATGGCTTGGTCCCTTGATTGAAGTGATTCGATTCTCCACCAAGTCCATCGAGCGAGAGATTAATTCAGTCAACGACAACCCATTGATTGATGTTTCAAGAAACAAAGCACTACATGGAGGCAACTTCCAAGGAACACCGATTGGAGTTTCTATGGACAACACTCGGTTGGCAATTGCTTCAATTGGAAAGCTCATGTTTGCCCAATTCTCAGAACTTGTCAACGATTTCTACAACAATGGTTTGCCTTCTAATCTTTCTGCAAGTAGAAATCCGAGCTTGGATTATGGGTTTAAAGGTGCTGAAATTGCCATGGCTTCATACTGCTCTGAGCTTCAATATCTTGCAAATCCAGTGACAAGTCATGTACAAAGTGCCGAGCAACACAATCAAGATGTTAACTCTCTTGGTTTAATTTCTTCAAGGAAAACTGTCGAAGCCATCGATATTCTGAAACTCATGTCATCTACATTTTTGGTTGCACTCTGTCAAGCAATTGACTTGAGGCATTTGGAGGAAAACTTGAAGAGCACTGTGAAAAGCACGGTTAGCCAAATAGCCAAGAAAGTGTTGACCACATCCTCTAATGGTGCACTTCACCCATCAAGATTTTGTGAGAAAGATCTACTTAAAGTTGTTGACAGGGAGTACACTTTTGCTTATATCGATGATCCATGCAGTGCTACTTACCCATTGAGTCAAAAGTTGAGACAAGTTCTTGTTGAACATGCACTTGCCAACGGTGAAGGTGAAAAGAACACAGACACCTCAATCTTCCAAAAGATTATTGCTTTCGAGGAGGAACTAAAAGCAGTATTACCAAAGGAGGTTGAAAATGCAAGGTTGTCATATGAGAGTGGAAATTCTAAAATTGAAAATCAGATCAAGGATTGCAGATCTTATCCATTGTATAGATTTGTGAGAGAAGAGTTGGGAACAAAATTGCTGACCGGAGAAAAAGTGATATCTCCAGGAGAAGAGTGCGAGAAGGTGTTCACAGCTTTGTGCCAAGGCAAGATGATTGACTCCATCTTAGAGTGCTTGAAGGAATGGAATGGTGCTCCAATTCCTATCTGCTAA

Coding sequence (CDS)

ATGGCACCTGCTCACTTCCAACAACTTGCCTGCCAGAATGGTTCCATTGAGAGCTTCTGCATCAGCCAAAATGATCCACTCAACTGGGGTGTGGCCGCCGAGTCCCTCAAGGGAAGCCATCTCGATGAGGTGAAGCGCATGGTAGAGGAGTACCGGAGGCCACTCGTCAAGCTTGGAGGTGAGACCCTCACCATTTCCCAGGTGGCTGCCATTGCTACTAGGGACTCTGATGTTACTGTTGAGCTTTCTGAATCTGCTAGAGCTGGTGTTAAAGCTAGTAGCGATTGGGTTATGGAGAGCATGAACAAAGGCACCGACAGTTATGGTGTTACTACTGGATTTGGTGCTACGTCTCATCGAAGGACCAAACAGGGTGGGGCTCTTCAGAAGGAGCTCATTCGATTCTTAAATGCTGGAATTTTTGGGAGCGGGGCTGAGTCGAATCATACTTTGCCTCACTCGGCTACGAGGGCGGCCATGTTGGTGAGAATCAATACTCTTCTTCAAGGCTACTCGGGTATTAGATTTGAGATTTTGGAAGCCATAACCAAACTCTTGAACCATAACATCACTCCATGCTTGCCTCTTCGTGGAACCATTACTGCTTCTGGAGACCTTGTTCCTCTTTCTTATATTGCCGGATTGCTCACTGGCCGACCAAACTCTAAGGCCATCGGGCCGAATGGAGAAAGTATTGATGCCAAGGAGGCTTTCCAACAAGCTGGTATTCCTTCTGGGTTCTTTGAGTTGCAGCCTAAGGAAGGTCTTGCACTTGTGAATGGCACCGCTGTTGGTTCTGGATTGGCTTCTATTGTCCTTTTTGAAGCAAATATTTTAGCCGTTTTGTCGGAAGTTCTTTCTGCCATTTTTGCTGAGGTCATGCAAGGAAAGCCTGAATTCACCGATCATTTGACTCATAAGTTGAAGCACCACCCTGGTCAGATTGAAGCGGCAGCAATCATGGAGCACATTTTGGATGGAAGTTCCTACATGAAAACAGCCAAGAAATTGCATGAAATTGATCCTCTCCAAAAGCCCAAACAAGATCGTTATGCTCTCAGGACTTCACCCCAATGGCTTGGTCCCTTGATTGAAGTGATTCGATTCTCCACCAAGTCCATCGAGCGAGAGATTAATTCAGTCAACGACAACCCATTGATTGATGTTTCAAGAAACAAAGCACTACATGGAGGCAACTTCCAAGGAACACCGATTGGAGTTTCTATGGACAACACTCGGTTGGCAATTGCTTCAATTGGAAAGCTCATGTTTGCCCAATTCTCAGAACTTGTCAACGATTTCTACAACAATGGTTTGCCTTCTAATCTTTCTGCAAGTAGAAATCCGAGCTTGGATTATGGGTTTAAAGGTGCTGAAATTGCCATGGCTTCATACTGCTCTGAGCTTCAATATCTTGCAAATCCAGTGACAAGTCATGTACAAAGTGCCGAGCAACACAATCAAGATGTTAACTCTCTTGGTTTAATTTCTTCAAGGAAAACTGTCGAAGCCATCGATATTCTGAAACTCATGTCATCTACATTTTTGGTTGCACTCTGTCAAGCAATTGACTTGAGGCATTTGGAGGAAAACTTGAAGAGCACTGTGAAAAGCACGGTTAGCCAAATAGCCAAGAAAGTGTTGACCACATCCTCTAATGGTGCACTTCACCCATCAAGATTTTGTGAGAAAGATCTACTTAAAGTTGTTGACAGGGAGTACACTTTTGCTTATATCGATGATCCATGCAGTGCTACTTACCCATTGAGTCAAAAGTTGAGACAAGTTCTTGTTGAACATGCACTTGCCAACGGTGAAGGTGAAAAGAACACAGACACCTCAATCTTCCAAAAGATTATTGCTTTCGAGGAGGAACTAAAAGCAGTATTACCAAAGGAGGTTGAAAATGCAAGGTTGTCATATGAGAGTGGAAATTCTAAAATTGAAAATCAGATCAAGGATTGCAGATCTTATCCATTGTATAGATTTGTGAGAGAAGAGTTGGGAACAAAATTGCTGACCGGAGAAAAAGTGATATCTCCAGGAGAAGAGTGCGAGAAGGTGTTCACAGCTTTGTGCCAAGGCAAGATGATTGACTCCATCTTAGAGTGCTTGAAGGAATGGAATGGTGCTCCAATTCCTATCTGCTAA

Protein sequence

MAPAHFQQLACQNGSIESFCISQNDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAIATRDSDVTVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFGSGAESNHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGESIDAKEAFQQAGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTVEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQIAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLSQKLRQVLVEHALANGEGEKNTDTSIFQKIIAFEEELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPLYRFVREELGTKLLTGEKVISPGEECEKVFTALCQGKMIDSILECLKEWNGAPIPIC
Homology
BLAST of Spg013422 vs. NCBI nr
Match: APO14269.1 (phenylalanine ammonia-lyase 3 [Luffa aegyptiaca])

HSP 1 Score: 1359.4 bits (3517), Expect = 0.0e+00
Identity = 693/700 (99.00%), Postives = 696/700 (99.43%), Query Frame = 0

Query: 17  ESFCISQNDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAIATRDS 76
           +S C S+NDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAIATRDS
Sbjct: 9   DSLCHSRNDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAIATRDS 68

Query: 77  DVTVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFL 136
           DVTVELSESARAGVKASSDWVMESMNKGT+SYGVTTGFGATSHRRTKQGGALQKELIRFL
Sbjct: 69  DVTVELSESARAGVKASSDWVMESMNKGTESYGVTTGFGATSHRRTKQGGALQKELIRFL 128

Query: 137 NAGIFGSGAESNHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCLPL 196
           NAGIFGSGAESNHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCLPL
Sbjct: 129 NAGIFGSGAESNHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCLPL 188

Query: 197 RGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGESIDAKEAFQQAGIPSGFFELQPKEGL 256
           RGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGESIDAKEAFQQAGIPSGFFELQPKEGL
Sbjct: 189 RGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGESIDAKEAFQQAGIPSGFFELQPKEGL 248

Query: 257 ALVNGTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEFTDHLTHKLKHHPGQIE 316
           ALVNGTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEFTDHLTHKLKHHPGQIE
Sbjct: 249 ALVNGTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEFTDHLTHKLKHHPGQIE 308

Query: 317 AAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIER 376
           AAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIER
Sbjct: 309 AAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIER 368

Query: 377 EINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYN 436
           EINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYN
Sbjct: 369 EINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYN 428

Query: 437 NGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLI 496
           NGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLI
Sbjct: 429 NGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLI 488

Query: 497 SSRKTVEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQIAKKVLTTSSNGAL 556
           SSRKT EAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQIAKKVLTTSSNGAL
Sbjct: 489 SSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQIAKKVLTTSSNGAL 548

Query: 557 HPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLSQKLRQVLVEHALANGEGEKNTDTSIF 616
           HPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLSQKLRQVLVEHALANGEGEKNTDTSIF
Sbjct: 549 HPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLSQKLRQVLVEHALANGEGEKNTDTSIF 608

Query: 617 QKIIAFEEELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPLYRFVREELGTKLLTGE 676
           QKII FEEELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPLYRFVREELGTKLLTGE
Sbjct: 609 QKIITFEEELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPLYRFVREELGTKLLTGE 668

Query: 677 KVISPGEECEKVFTALCQGKMIDSILECLKEWNGAPIPIC 717
           KVISPGEECEKVFTALCQGKMIDSILECLKEWNGAPIPIC
Sbjct: 669 KVISPGEECEKVFTALCQGKMIDSILECLKEWNGAPIPIC 708

BLAST of Spg013422 vs. NCBI nr
Match: XP_038881628.1 (phenylalanine ammonia-lyase-like [Benincasa hispida])

HSP 1 Score: 1349.3 bits (3491), Expect = 0.0e+00
Identity = 682/716 (95.25%), Postives = 701/716 (97.91%), Query Frame = 0

Query: 1   MAPAHFQQLACQNGSIESFCISQNDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGG 60
           MAP HFQQ   QNGS+ES CISQNDPLNWGVAA+SLKGSHLDEVK M+EEYRRP+VK+GG
Sbjct: 1   MAPTHFQQ---QNGSMESLCISQNDPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGG 60

Query: 61  ETLTISQVAAIATRDSDVTVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR 120
           E+LTISQVAAIATRDSDV VELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR
Sbjct: 61  ESLTISQVAAIATRDSDVIVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR 120

Query: 121 RTKQGGALQKELIRFLNAGIFGSGAESNHTLPHSATRAAMLVRINTLLQGYSGIRFEILE 180
           RTKQGGALQKELIRFLNAGIFG+GAES+HTLPHSATRAAMLVRINTLLQGYSGIRFEILE
Sbjct: 121 RTKQGGALQKELIRFLNAGIFGNGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILE 180

Query: 181 AITKLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGESIDAKEAFQQ 240
           AITKLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGE +DAKEAFQQ
Sbjct: 181 AITKLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQ 240

Query: 241 AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEF 300
           AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSE+LSAIFAEVMQGKPEF
Sbjct: 241 AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEF 300

Query: 301 TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWL 360
           TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWL
Sbjct: 301 TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWL 360

Query: 361 GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIG 420
           GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIG
Sbjct: 361 GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIG 420

Query: 421 KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV 480
           KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV
Sbjct: 421 KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV 480

Query: 481 QSAEQHNQDVNSLGLISSRKTVEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTV 540
           QSAEQHNQDVNSLGLISSRKT EAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTV
Sbjct: 481 QSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTV 540

Query: 541 SQIAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLSQKLRQVLVEH 600
           SQ+AKKVLTT SNGALHPSRFCEKDLLKVVDREYTFAYIDDPC+ATYPLSQKLRQVLVEH
Sbjct: 541 SQVAKKVLTTCSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEH 600

Query: 601 ALANGEGEKNTDTSIFQKIIAFEEELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPL 660
           ALANG+ EKN DTSIFQKI+ FEEELKA+LPKEVENARL+YE+GNSKIENQIKDCRSYP+
Sbjct: 601 ALANGDDEKNADTSIFQKIVLFEEELKALLPKEVENARLAYETGNSKIENQIKDCRSYPI 660

Query: 661 YRFVREELGTKLLTGEKVISPGEECEKVFTALCQGKMIDSILECLKEWNGAPIPIC 717
           YRFVREEL TK+LTGEKVISPGEECEKVF ALCQGKMIDSILECLKEWNGAPIPIC
Sbjct: 661 YRFVREELETKMLTGEKVISPGEECEKVFIALCQGKMIDSILECLKEWNGAPIPIC 713

BLAST of Spg013422 vs. NCBI nr
Match: XP_023518835.1 (phenylalanine ammonia-lyase-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1346.3 bits (3483), Expect = 0.0e+00
Identity = 681/716 (95.11%), Postives = 698/716 (97.49%), Query Frame = 0

Query: 1   MAPAHFQQLACQNGSIESFCISQNDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGG 60
           MAPA FQQLA QNGS+E+FCIS NDPLNWGVAA++LKGSHLDEVKRMVEEYRRPLVKLGG
Sbjct: 1   MAPAPFQQLAAQNGSVETFCISHNDPLNWGVAADALKGSHLDEVKRMVEEYRRPLVKLGG 60

Query: 61  ETLTISQVAAIATRDSDVTVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR 120
           ETLTISQVAAIATRD+DV VEL+ESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR
Sbjct: 61  ETLTISQVAAIATRDNDVIVELAESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR 120

Query: 121 RTKQGGALQKELIRFLNAGIFGSGAESNHTLPHSATRAAMLVRINTLLQGYSGIRFEILE 180
           RT QGGALQKELIRFLNAGIFGSGAES HTLPHSATRAAMLVRINTLLQGYSGIRFEILE
Sbjct: 121 RTNQGGALQKELIRFLNAGIFGSGAESTHTLPHSATRAAMLVRINTLLQGYSGIRFEILE 180

Query: 181 AITKLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGESIDAKEAFQQ 240
           AITKLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKA+GPNGES+DA EAFQQ
Sbjct: 181 AITKLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAVGPNGESLDAMEAFQQ 240

Query: 241 AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEF 300
           AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSE+LSAIFAEVMQGKPEF
Sbjct: 241 AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEF 300

Query: 301 TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWL 360
           TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHE DPLQKPKQDRYALRTSPQWL
Sbjct: 301 TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHETDPLQKPKQDRYALRTSPQWL 360

Query: 361 GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIG 420
           GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKA+HGGNFQGTPIGVSMDNTRLA+ASIG
Sbjct: 361 GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKAIHGGNFQGTPIGVSMDNTRLAVASIG 420

Query: 421 KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV 480
           KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV
Sbjct: 421 KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV 480

Query: 481 QSAEQHNQDVNSLGLISSRKTVEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTV 540
           QSAEQHNQDVNSLGLISSRKT EA+DILKLMSSTFLVALCQAIDLRHLEENLK+TVK+ V
Sbjct: 481 QSAEQHNQDVNSLGLISSRKTAEALDILKLMSSTFLVALCQAIDLRHLEENLKNTVKNCV 540

Query: 541 SQIAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLSQKLRQVLVEH 600
           SQ AKKVL TSSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLSQKLRQVLVEH
Sbjct: 541 SQTAKKVLITSSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLSQKLRQVLVEH 600

Query: 601 ALANGEGEKNTDTSIFQKIIAFEEELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPL 660
           AL NG+ EK+ DTSIFQKI AFEEELKAVLPKEVENARL YESGNSKIENQIKDCRSYPL
Sbjct: 601 ALGNGDCEKDADTSIFQKIGAFEEELKAVLPKEVENARLWYESGNSKIENQIKDCRSYPL 660

Query: 661 YRFVREELGTKLLTGEKVISPGEECEKVFTALCQGKMIDSILECLKEWNGAPIPIC 717
           YRFVREELGT LLTGEKVISPGEECEKVFTALC+GKMIDSILECLKEWNGAPIPIC
Sbjct: 661 YRFVREELGTNLLTGEKVISPGEECEKVFTALCEGKMIDSILECLKEWNGAPIPIC 716

BLAST of Spg013422 vs. NCBI nr
Match: XP_023518834.1 (phenylalanine ammonia-lyase-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1345.1 bits (3480), Expect = 0.0e+00
Identity = 680/716 (94.97%), Postives = 698/716 (97.49%), Query Frame = 0

Query: 1   MAPAHFQQLACQNGSIESFCISQNDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGG 60
           MAPA FQQLA QNGS+E+FCIS NDPLNWGVAA++LKGSHLDEVKRMVEEYRRPLVKLGG
Sbjct: 1   MAPAPFQQLAAQNGSVETFCISHNDPLNWGVAADALKGSHLDEVKRMVEEYRRPLVKLGG 60

Query: 61  ETLTISQVAAIATRDSDVTVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR 120
           ETLTISQVAAIATRD+DV VEL+ESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR
Sbjct: 61  ETLTISQVAAIATRDNDVIVELAESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR 120

Query: 121 RTKQGGALQKELIRFLNAGIFGSGAESNHTLPHSATRAAMLVRINTLLQGYSGIRFEILE 180
           RT QGGALQKELIRFLNAGIFGSGAES HTLPHSATRAAMLVRINTLLQGYSGIRFEILE
Sbjct: 121 RTNQGGALQKELIRFLNAGIFGSGAESTHTLPHSATRAAMLVRINTLLQGYSGIRFEILE 180

Query: 181 AITKLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGESIDAKEAFQQ 240
           AITKLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKA+GPNGES+DA EAFQQ
Sbjct: 181 AITKLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAVGPNGESLDAMEAFQQ 240

Query: 241 AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEF 300
           AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSE+LSAIFAEVMQGKPEF
Sbjct: 241 AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEF 300

Query: 301 TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWL 360
           TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHE DPLQKPKQDRYALRTSPQWL
Sbjct: 301 TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHETDPLQKPKQDRYALRTSPQWL 360

Query: 361 GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIG 420
           GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKA+HGGNFQGTPIGVSMDNTRLA+ASIG
Sbjct: 361 GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKAIHGGNFQGTPIGVSMDNTRLAVASIG 420

Query: 421 KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV 480
           KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV
Sbjct: 421 KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV 480

Query: 481 QSAEQHNQDVNSLGLISSRKTVEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTV 540
           QSAEQHNQDVNSLGLISSRKT EA+DILKLMSSTFLVALCQAIDLRHLEENLK+TVK+ V
Sbjct: 481 QSAEQHNQDVNSLGLISSRKTAEALDILKLMSSTFLVALCQAIDLRHLEENLKNTVKNCV 540

Query: 541 SQIAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLSQKLRQVLVEH 600
           SQ AKKVL TSSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLSQKLRQVLVEH
Sbjct: 541 SQTAKKVLITSSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLSQKLRQVLVEH 600

Query: 601 ALANGEGEKNTDTSIFQKIIAFEEELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPL 660
           AL NG+ EK+ DTSIFQKI AFEEELKAVLPKEVENARLSY SGNSKIENQIKDCRSYPL
Sbjct: 601 ALGNGDCEKDADTSIFQKIGAFEEELKAVLPKEVENARLSYGSGNSKIENQIKDCRSYPL 660

Query: 661 YRFVREELGTKLLTGEKVISPGEECEKVFTALCQGKMIDSILECLKEWNGAPIPIC 717
           YRFVREELGT L+TGEKVISPGEECEKVFTALC+GKMIDSILECLKEWNGAPIPIC
Sbjct: 661 YRFVREELGTNLVTGEKVISPGEECEKVFTALCEGKMIDSILECLKEWNGAPIPIC 716

BLAST of Spg013422 vs. NCBI nr
Match: KAG6595242.1 (Phenylalanine ammonia-lyase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1343.9 bits (3477), Expect = 0.0e+00
Identity = 680/716 (94.97%), Postives = 697/716 (97.35%), Query Frame = 0

Query: 1   MAPAHFQQLACQNGSIESFCISQNDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGG 60
           MAPA FQQLA QNGS+ESFC ++NDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGG
Sbjct: 1   MAPAPFQQLAAQNGSVESFCTTRNDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGG 60

Query: 61  ETLTISQVAAIATRDSDVTVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR 120
           ETLTISQVAAIATRD+DV VEL+ESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR
Sbjct: 61  ETLTISQVAAIATRDNDVMVELAESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR 120

Query: 121 RTKQGGALQKELIRFLNAGIFGSGAESNHTLPHSATRAAMLVRINTLLQGYSGIRFEILE 180
           RT QGGALQKELIRFLNAGIFGSGAES HTLPHSATRAAMLVRINTLLQGYSGIRFEILE
Sbjct: 121 RTNQGGALQKELIRFLNAGIFGSGAESTHTLPHSATRAAMLVRINTLLQGYSGIRFEILE 180

Query: 181 AITKLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGESIDAKEAFQQ 240
           AITKLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKA+G NGES+DAKEAFQQ
Sbjct: 181 AITKLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAVGQNGESLDAKEAFQQ 240

Query: 241 AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEF 300
           AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSE+LSAIFAEVMQGKPEF
Sbjct: 241 AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEF 300

Query: 301 TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWL 360
           TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMK AKKLHE DPLQKPKQDRYALRTSPQWL
Sbjct: 301 TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKAAKKLHETDPLQKPKQDRYALRTSPQWL 360

Query: 361 GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIG 420
           GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKA+HGGNFQGTPIGVSMDNTRLA+ASIG
Sbjct: 361 GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKAIHGGNFQGTPIGVSMDNTRLAVASIG 420

Query: 421 KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV 480
           KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV
Sbjct: 421 KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV 480

Query: 481 QSAEQHNQDVNSLGLISSRKTVEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTV 540
           QSAEQHNQDVNSLGLISSRKT EA+DILKLMSSTFLVALCQAIDLRHLEENLKSTVK+ V
Sbjct: 481 QSAEQHNQDVNSLGLISSRKTAEALDILKLMSSTFLVALCQAIDLRHLEENLKSTVKNCV 540

Query: 541 SQIAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLSQKLRQVLVEH 600
           SQ AKKVLTTSSNGALHPSRFCEKDLLKVVDRE+TFAYIDDPCSATYPLSQKLRQVLVEH
Sbjct: 541 SQTAKKVLTTSSNGALHPSRFCEKDLLKVVDREHTFAYIDDPCSATYPLSQKLRQVLVEH 600

Query: 601 ALANGEGEKNTDTSIFQKIIAFEEELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPL 660
           AL NG+ EK+ DTSIFQKI AFEEELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPL
Sbjct: 601 ALGNGDCEKDADTSIFQKIGAFEEELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPL 660

Query: 661 YRFVREELGTKLLTGEKVISPGEECEKVFTALCQGKMIDSILECLKEWNGAPIPIC 717
           YRFVREELGT LLTGEK ISPGEECEKVFTALC+GKMID +LECLKEWNGAPIPIC
Sbjct: 661 YRFVREELGTNLLTGEKAISPGEECEKVFTALCEGKMIDCMLECLKEWNGAPIPIC 716

BLAST of Spg013422 vs. ExPASy Swiss-Prot
Match: P45730 (Phenylalanine ammonia-lyase OS=Populus trichocarpa OX=3694 GN=PAL PE=2 SV=1)

HSP 1 Score: 1227.2 bits (3174), Expect = 0.0e+00
Identity = 612/706 (86.69%), Postives = 665/706 (94.19%), Query Frame = 0

Query: 12  QNGSIESFCISQNDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAI 71
           QNGS+ES C++Q DPL+WGVAAE++KGSHLDEVKRMV +YR+P+VKLGGETLTI+QVA+I
Sbjct: 10  QNGSLESLCVNQRDPLSWGVAAEAMKGSHLDEVKRMVADYRKPVVKLGGETLTIAQVASI 69

Query: 72  ATRDS-DVTVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQK 131
           A  D+ DV VELSESAR GVKASSDWVM+SM+KGTDSYGVTTGFGATSHRRTKQGGALQK
Sbjct: 70  AGHDTGDVKVELSESARPGVKASSDWVMDSMDKGTDSYGVTTGFGATSHRRTKQGGALQK 129

Query: 132 ELIRFLNAGIFGSGAESNHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNI 191
           ELIRFLNAGIFG+G E+ HTLPHSATRAAMLVRINTLLQGYSGIRFEILEAIT+LLN+NI
Sbjct: 130 ELIRFLNAGIFGNGTETCHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITRLLNNNI 189

Query: 192 TPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGESIDAKEAFQQAGIPSGFFEL 251
           TPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKA GP GE +DA EAF+ AGI SGFFEL
Sbjct: 190 TPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKATGPTGEVLDAAEAFKAAGIESGFFEL 249

Query: 252 QPKEGLALVNGTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEFTDHLTHKLKH 311
           QPKEGLALVNGTAVGSGLAS+VLFE N+LAVLSE+LSAIFAEVM GKPEFTDHLTHKLKH
Sbjct: 250 QPKEGLALVNGTAVGSGLASMVLFETNVLAVLSELLSAIFAEVMNGKPEFTDHLTHKLKH 309

Query: 312 HPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFS 371
           HPGQIEAAAIMEHILDGS+YMK AKKLHE DPLQKPKQDRYALRTSPQWLGP IEVIRFS
Sbjct: 310 HPGQIEAAAIMEHILDGSAYMKAAKKLHETDPLQKPKQDRYALRTSPQWLGPQIEVIRFS 369

Query: 372 TKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSEL 431
           TKSIEREINSVNDNPLIDVSRNKA+HGGNFQGTPIGVSMDN RLAIASIGKL+FAQFSEL
Sbjct: 370 TKSIEREINSVNDNPLIDVSRNKAIHGGNFQGTPIGVSMDNVRLAIASIGKLLFAQFSEL 429

Query: 432 VNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDV 491
           VNDFYNNGLPSNL+ASRNPSLDYGFKGAEIAMASYCSELQYLANPVT+HVQSAEQHNQDV
Sbjct: 430 VNDFYNNGLPSNLTASRNPSLDYGFKGAEIAMASYCSELQYLANPVTTHVQSAEQHNQDV 489

Query: 492 NSLGLISSRKTVEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQIAKKVLTT 551
           NSLGLISSRKT EA+DILKLMS+TFLVALCQAIDLRHLEENLKS VK+TVSQ++K+VLTT
Sbjct: 490 NSLGLISSRKTAEAVDILKLMSTTFLVALCQAIDLRHLEENLKSAVKNTVSQVSKRVLTT 549

Query: 552 SSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLSQKLRQVLVEHALANGEGEKN 611
            +NG LHPSRFCEK+LLKVVDREY FAY+DDPCSATYPL QKLRQV V+HAL NGE EKN
Sbjct: 550 GANGELHPSRFCEKELLKVVDREYVFAYVDDPCSATYPLMQKLRQVFVDHALENGENEKN 609

Query: 612 TDTSIFQKIIAFEEELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPLYRFVREELGT 671
             TS+FQKI AFEEELKA+LPKEVE+AR +Y+SGNS I+N+IK+CRSYPLY+FVREELGT
Sbjct: 610 FSTSVFQKIEAFEEELKALLPKEVESARAAYDSGNSAIDNKIKECRSYPLYKFVREELGT 669

Query: 672 KLLTGEKVISPGEECEKVFTALCQGKMIDSILECLKEWNGAPIPIC 717
            LLTGEKV SPGEE +KVFTA+CQGK+ID +LECL EWNG+P+PIC
Sbjct: 670 VLLTGEKVQSPGEEFDKVFTAMCQGKIIDPMLECLGEWNGSPLPIC 715

BLAST of Spg013422 vs. ExPASy Swiss-Prot
Match: P45732 (Phenylalanine ammonia-lyase OS=Stylosanthes humilis OX=35628 GN=PAL17.1 PE=2 SV=1)

HSP 1 Score: 1223.8 bits (3165), Expect = 0.0e+00
Identity = 609/706 (86.26%), Postives = 660/706 (93.48%), Query Frame = 0

Query: 18  SFCISQN-------DPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAA 77
           +FC++ N       DPLNW  AAE+LKGSHLDEVKRMV EYR+PLV LGG+TLTISQVAA
Sbjct: 10  TFCLTANNGQQPRHDPLNWAAAAEALKGSHLDEVKRMVSEYRKPLVNLGGQTLTISQVAA 69

Query: 78  IATRDSDVTVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQK 137
           IA  D  V+V+LSE++RAGVKASSDWVM+SMN GTDSYGVTTGFGATSHRRTKQGGALQK
Sbjct: 70  IAANDQGVSVQLSEASRAGVKASSDWVMDSMNNGTDSYGVTTGFGATSHRRTKQGGALQK 129

Query: 138 ELIRFLNAGIFGSGAESNHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNI 197
           ELIRFLNAGIFG+G E+N TLPH+ATRAAMLVRINTLLQGYSGIRFEILEAITKLLN+NI
Sbjct: 130 ELIRFLNAGIFGNGTETNCTLPHTATRAAMLVRINTLLQGYSGIRFEILEAITKLLNNNI 189

Query: 198 TPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGESIDAKEAFQQAGIPSGFFEL 257
           TPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKA+GPNGE+++AKEAFQ AGI S FFEL
Sbjct: 190 TPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAVGPNGETLNAKEAFQAAGIGSDFFEL 249

Query: 258 QPKEGLALVNGTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEFTDHLTHKLKH 317
           QPKEGLALVNGT VGSGLAS+VLFEANILAVLSEVLSAIFAEVMQGKPEFTDHLTHKLKH
Sbjct: 250 QPKEGLALVNGTPVGSGLASVVLFEANILAVLSEVLSAIFAEVMQGKPEFTDHLTHKLKH 309

Query: 318 HPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFS 377
           HPGQIEAAAIMEHILDGSSY+K AKKLHEIDPLQKPKQDRYALRTSPQWLGPL+EVIRFS
Sbjct: 310 HPGQIEAAAIMEHILDGSSYVKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPLVEVIRFS 369

Query: 378 TKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSEL 437
           TKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLA+ASIGKLMFAQFSEL
Sbjct: 370 TKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAVASIGKLMFAQFSEL 429

Query: 438 VNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDV 497
           VNDFYNNGLPSNLSASRNPSLDYGFKG EIAMASYCSELQYLANPVTSHVQSAEQHNQDV
Sbjct: 430 VNDFYNNGLPSNLSASRNPSLDYGFKGTEIAMASYCSELQYLANPVTSHVQSAEQHNQDV 489

Query: 498 NSLGLISSRKTVEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQIAKKVLTT 557
           NSLGLIS+RKT EA++ILKLMS T+L+ALCQAIDLRHLEENLK+TVK+TVSQ+AK+ LTT
Sbjct: 490 NSLGLISARKTNEAVEILKLMSPTYLIALCQAIDLRHLEENLKNTVKNTVSQVAKRTLTT 549

Query: 558 SSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLSQKLRQVLVEHALANGEGEKN 617
             NG LHPSRFCEKDLLK+VDREY FAYIDDPCSATYPL QKLRQVLVEHALAN E EKN
Sbjct: 550 GVNGELHPSRFCEKDLLKIVDREYCFAYIDDPCSATYPLMQKLRQVLVEHALANAENEKN 609

Query: 618 TDTSIFQKIIAFEEELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPLYRFVREELGT 677
            +TSIFQKI  FEEELK +LPKEVE AR++YE+G S I N+IK+CRSYPLY+FVREELGT
Sbjct: 610 VNTSIFQKITTFEEELKTLLPKEVEGARIAYENGQSAIPNKIKECRSYPLYKFVREELGT 669

Query: 678 KLLTGEKVISPGEECEKVFTALCQGKMIDSILECLKEWNGAPIPIC 717
           ++LTGEKV SPGEEC+K+FTA+CQGK+ID +LEC+ EWNGAP+P+C
Sbjct: 670 EMLTGEKVRSPGEECDKLFTAMCQGKIIDPLLECIGEWNGAPLPLC 715

BLAST of Spg013422 vs. ExPASy Swiss-Prot
Match: P45734 (Phenylalanine ammonia-lyase OS=Trifolium subterraneum OX=3900 GN=PAL1 PE=3 SV=1)

HSP 1 Score: 1218.0 bits (3150), Expect = 0.0e+00
Identity = 611/696 (87.79%), Postives = 660/696 (94.83%), Query Frame = 0

Query: 21  ISQNDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAIATRDSDVTV 80
           ++  DPLNWGVAAE++KGSHLDEVKRMVEEYR+P+V+LGGETLTISQVAAIA  D   TV
Sbjct: 31  VNAADPLNWGVAAEAMKGSHLDEVKRMVEEYRKPVVRLGGETLTISQVAAIAAHDG-ATV 90

Query: 81  ELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGI 140
           ELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGI
Sbjct: 91  ELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGI 150

Query: 141 FGSGAESNHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCLPLRGTI 200
           FG+G ESNHTLPH+ATRAAMLVRINTLLQGYSGIRFEILEAITKLLN+NITPCLPLRGTI
Sbjct: 151 FGNGTESNHTLPHTATRAAMLVRINTLLQGYSGIRFEILEAITKLLNNNITPCLPLRGTI 210

Query: 201 TASGDLVPLSYIAGLLTGRPNSKAIGPNGESIDAKEAFQQAGIPSGFFELQPKEGLALVN 260
           TASGDLVPLSYIAGLLTGR NSKA GP+GE ++AKEAFQ AGI + FFELQPKEGLALVN
Sbjct: 211 TASGDLVPLSYIAGLLTGRSNSKAHGPSGEMLNAKEAFQLAGINAEFFELQPKEGLALVN 270

Query: 261 GTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAI 320
           GTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAI
Sbjct: 271 GTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAI 330

Query: 321 MEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIEREINS 380
           MEHIL GS+Y+K AKKLHE+DPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIEREINS
Sbjct: 331 MEHILHGSAYVKDAKKLHEMDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIEREINS 390

Query: 381 VNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYNNGLP 440
           VNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLA+ASIGKL+FAQFSELVNDFYNNGLP
Sbjct: 391 VNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLALASIGKLLFAQFSELVNDFYNNGLP 450

Query: 441 SNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRK 500
           SNLSASRNPSLDYGFKG+EIAMASYCSELQYLANPVT+HVQSAEQHNQDVNSLGLISSRK
Sbjct: 451 SNLSASRNPSLDYGFKGSEIAMASYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISSRK 510

Query: 501 TVEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQIAKKVLTTSSNGALHPSR 560
           T EAI+IL+LMSSTFL+ALCQAIDLRHLEENLK++VK+TVSQ+AKK LT   +G LHPSR
Sbjct: 511 TKEAIEILQLMSSTFLIALCQAIDLRHLEENLKNSVKNTVSQVAKKTLTIGVSGELHPSR 570

Query: 561 FCEKDLLKVVDREYTFAYIDDPCSATYPLSQKLRQVLVEHALANGEGEKNTDTSIFQKII 620
           FCEKDLLKVVDRE+ F+YIDDPCSATYPL+QKLRQVLV+HAL NGE EKN++TSIFQKI 
Sbjct: 571 FCEKDLLKVVDREHVFSYIDDPCSATYPLAQKLRQVLVDHALVNGESEKNSNTSIFQKIA 630

Query: 621 AFEEELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPLYRFVREELGTKLLTGEKVIS 680
            FEEELK +LPKEVE+AR +YE+GNS I N+I  CRSYPLY+FVREELGT LLTGE+VIS
Sbjct: 631 TFEEELKTLLPKEVESARTAYENGNSTIANKINGCRSYPLYKFVREELGTSLLTGERVIS 690

Query: 681 PGEECEKVFTALCQGKMIDSILECLKEWNGAPIPIC 717
           PGEEC+K+FTA+CQGK+ID +L+CL EWNGAP+PIC
Sbjct: 691 PGEECDKLFTAMCQGKIIDPLLKCLGEWNGAPLPIC 725

BLAST of Spg013422 vs. ExPASy Swiss-Prot
Match: Q04593 (Phenylalanine ammonia-lyase 2 OS=Pisum sativum OX=3888 GN=PAL2 PE=2 SV=1)

HSP 1 Score: 1210.7 bits (3131), Expect = 0.0e+00
Identity = 608/713 (85.27%), Postives = 663/713 (92.99%), Query Frame = 0

Query: 13  NGSIESFC----------ISQNDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGET 72
           N   +SFC          +S +DPLNWGVAAE++KGSHLDEVKRMV+EYR+P+V+LGGET
Sbjct: 12  NSGYDSFCLTNAKNNNIKVSDSDPLNWGVAAEAMKGSHLDEVKRMVDEYRKPVVRLGGET 71

Query: 73  LTISQVAAIATRDSDVTVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRT 132
           LTISQVAAIA  D  V VELSESARAGVKASSDWVMESMNKGTDSYGVTT  GATSHRRT
Sbjct: 72  LTISQVAAIAAHDHGVKVELSESARAGVKASSDWVMESMNKGTDSYGVTTVHGATSHRRT 131

Query: 133 KQGGALQKELIRFLNAGIFGSGAESNHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAI 192
           KQGGALQKELIRFLNAGIFG+G+ES HTLPH+ATRAAMLVRINTLLQGYSGIRFEILEAI
Sbjct: 132 KQGGALQKELIRFLNAGIFGNGSESTHTLPHTATRAAMLVRINTLLQGYSGIRFEILEAI 191

Query: 193 TKLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGESIDAKEAFQQAG 252
           TKL+N+N+TPCL LRGTITASGDLVPLSYIAGLLTGRPNSKA GP+GE ++A+EAFQ AG
Sbjct: 192 TKLINNNVTPCL-LRGTITASGDLVPLSYIAGLLTGRPNSKAHGPSGEILNAREAFQSAG 251

Query: 253 IPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEFTD 312
           I  GFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEFTD
Sbjct: 252 INDGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEFTD 311

Query: 313 HLTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGP 372
           HLTHKLKHHPGQIEAAAIMEHILDGS+Y+K AKKLHE+DPLQKPKQDRYALRTSPQWLGP
Sbjct: 312 HLTHKLKHHPGQIEAAAIMEHILDGSAYVKAAKKLHEMDPLQKPKQDRYALRTSPQWLGP 371

Query: 373 LIEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKL 432
           LIEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLA+ASIGKL
Sbjct: 372 LIEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLALASIGKL 431

Query: 433 MFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQS 492
           +FAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKG+EIAMASYCSELQYLANPVT+HVQS
Sbjct: 432 LFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGSEIAMASYCSELQYLANPVTTHVQS 491

Query: 493 AEQHNQDVNSLGLISSRKTVEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQ 552
           AEQHNQDVNSLGLISSRKT EAI+IL+LMSSTFL+ALCQAIDLRHLEENLK++VK+ VS 
Sbjct: 492 AEQHNQDVNSLGLISSRKTYEAIEILQLMSSTFLIALCQAIDLRHLEENLKNSVKNMVSH 551

Query: 553 IAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLSQKLRQVLVEHAL 612
           +AK+ LTT  NG LHPSRFCEKDLL+VVDRE+ F+YIDDPCSATYPL QKLRQVLV+HAL
Sbjct: 552 VAKRTLTTGINGELHPSRFCEKDLLRVVDREHVFSYIDDPCSATYPLMQKLRQVLVDHAL 611

Query: 613 ANGEGEKNTDTSIFQKIIAFEEELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPLYR 672
            NGE EKN +TSIFQKI  FE+ELK +LPKEVE+AR +YE+GN+ I N+IK+CRSYPLY+
Sbjct: 612 VNGESEKNLNTSIFQKIATFEDELKTLLPKEVESARGAYENGNTTISNKIKECRSYPLYK 671

Query: 673 FVREELGTKLLTGEKVISPGEECEKVFTALCQGKMIDSILECLKEWNGAPIPI 716
           FVREELGT LLTGEKVISPGEEC+K+FTA+CQGK+ID +LECL +WNGAP+PI
Sbjct: 672 FVREELGTSLLTGEKVISPGEECDKLFTAICQGKIIDPLLECLGDWNGAPLPI 723

BLAST of Spg013422 vs. ExPASy Swiss-Prot
Match: P27990 (Phenylalanine ammonia-lyase OS=Medicago sativa OX=3879 PE=2 SV=1)

HSP 1 Score: 1210.3 bits (3130), Expect = 0.0e+00
Identity = 612/716 (85.47%), Postives = 658/716 (91.90%), Query Frame = 0

Query: 12  QNGSIESFC-----------ISQNDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGG 71
           +N + ESFC           +++ DPLNWGVAAE++KGSHLDEVKRMV EYR+P+V+LGG
Sbjct: 10  KNNANESFCLIHAKNNNNMKVNEADPLNWGVAAEAMKGSHLDEVKRMVAEYRKPVVRLGG 69

Query: 72  ETLTISQVAAIATRDSDVTVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR 131
           ETLTISQVAAIA  D  V V+LSESAR GVKASS+WVMESMNKGTDSYGVTTGFGATSH 
Sbjct: 70  ETLTISQVAAIAAHDHGVQVDLSESARDGVKASSEWVMESMNKGTDSYGVTTGFGATSHS 129

Query: 132 RTKQGGALQKELIRFLNAGIFGSGAESNHTLPHSATRAAMLVRINTLLQGYSGIRFEILE 191
           RTKQGGALQKELIRFLNAGIFG+G ESNHTLP +ATRAAMLVRINTLLQGYSGI FEILE
Sbjct: 130 RTKQGGALQKELIRFLNAGIFGNGTESNHTLPKTATRAAMLVRINTLLQGYSGIDFEILE 189

Query: 192 AITKLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGESIDAKEAFQQ 251
           AITK LN  +TPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKA GP+GE ++AKEAF  
Sbjct: 190 AITKPLNKTVTPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAHGPSGEVLNAKEAFNL 249

Query: 252 AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEF 311
           AGI + FFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEF
Sbjct: 250 AGINAEFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEF 309

Query: 312 TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWL 371
           TDHLTHKLKHHPGQIEAAAIMEHILDGSSY+K AKKLHEIDPLQKPKQDRYALRTSPQWL
Sbjct: 310 TDHLTHKLKHHPGQIEAAAIMEHILDGSSYVKAAKKLHEIDPLQKPKQDRYALRTSPQWL 369

Query: 372 GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIG 431
           GPL+EVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLA+ASIG
Sbjct: 370 GPLVEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLALASIG 429

Query: 432 KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV 491
           KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVT+HV
Sbjct: 430 KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTTHV 489

Query: 492 QSAEQHNQDVNSLGLISSRKTVEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTV 551
           QSAEQHNQDVNSLGLIS+RKT EAI+IL+LMSSTFL+ALCQAIDLRHLEENLK++VK+TV
Sbjct: 490 QSAEQHNQDVNSLGLISARKTNEAIEILQLMSSTFLIALCQAIDLRHLEENLKNSVKNTV 549

Query: 552 SQIAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLSQKLRQVLVEH 611
           SQ+AKK LT   NG LHPSRFCEKDLLKVVDRE+ FAYIDDPCSATYPLSQKLRQVLV+H
Sbjct: 550 SQVAKKTLTMGVNGELHPSRFCEKDLLKVVDREHVFAYIDDPCSATYPLSQKLRQVLVDH 609

Query: 612 ALANGEGEKNTDTSIFQKIIAFEEELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPL 671
           AL NGE EKN +TSIFQKI  FEEELK +LPKEVE+AR +YESGN  I N+I  CRSYPL
Sbjct: 610 ALVNGESEKNFNTSIFQKIATFEEELKTLLPKEVESARTAYESGNPTIPNKINGCRSYPL 669

Query: 672 YRFVREELGTKLLTGEKVISPGEECEKVFTALCQGKMIDSILECLKEWNGAPIPIC 717
           Y+FVREELGT LLTGE VISPGEEC+K+F+A+CQGK+ID +LECL EWNGAP+PIC
Sbjct: 670 YKFVREELGTGLLTGENVISPGEECDKLFSAMCQGKIIDPLLECLGEWNGAPLPIC 725

BLAST of Spg013422 vs. ExPASy TrEMBL
Match: A0A1L5JHV3 (Phenylalanine ammonia-lyase OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 1359.4 bits (3517), Expect = 0.0e+00
Identity = 693/700 (99.00%), Postives = 696/700 (99.43%), Query Frame = 0

Query: 17  ESFCISQNDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAIATRDS 76
           +S C S+NDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAIATRDS
Sbjct: 9   DSLCHSRNDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAIATRDS 68

Query: 77  DVTVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFL 136
           DVTVELSESARAGVKASSDWVMESMNKGT+SYGVTTGFGATSHRRTKQGGALQKELIRFL
Sbjct: 69  DVTVELSESARAGVKASSDWVMESMNKGTESYGVTTGFGATSHRRTKQGGALQKELIRFL 128

Query: 137 NAGIFGSGAESNHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCLPL 196
           NAGIFGSGAESNHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCLPL
Sbjct: 129 NAGIFGSGAESNHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCLPL 188

Query: 197 RGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGESIDAKEAFQQAGIPSGFFELQPKEGL 256
           RGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGESIDAKEAFQQAGIPSGFFELQPKEGL
Sbjct: 189 RGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGESIDAKEAFQQAGIPSGFFELQPKEGL 248

Query: 257 ALVNGTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEFTDHLTHKLKHHPGQIE 316
           ALVNGTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEFTDHLTHKLKHHPGQIE
Sbjct: 249 ALVNGTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEFTDHLTHKLKHHPGQIE 308

Query: 317 AAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIER 376
           AAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIER
Sbjct: 309 AAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIER 368

Query: 377 EINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYN 436
           EINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYN
Sbjct: 369 EINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYN 428

Query: 437 NGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLI 496
           NGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLI
Sbjct: 429 NGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLI 488

Query: 497 SSRKTVEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQIAKKVLTTSSNGAL 556
           SSRKT EAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQIAKKVLTTSSNGAL
Sbjct: 489 SSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQIAKKVLTTSSNGAL 548

Query: 557 HPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLSQKLRQVLVEHALANGEGEKNTDTSIF 616
           HPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLSQKLRQVLVEHALANGEGEKNTDTSIF
Sbjct: 549 HPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLSQKLRQVLVEHALANGEGEKNTDTSIF 608

Query: 617 QKIIAFEEELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPLYRFVREELGTKLLTGE 676
           QKII FEEELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPLYRFVREELGTKLLTGE
Sbjct: 609 QKIITFEEELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPLYRFVREELGTKLLTGE 668

Query: 677 KVISPGEECEKVFTALCQGKMIDSILECLKEWNGAPIPIC 717
           KVISPGEECEKVFTALCQGKMIDSILECLKEWNGAPIPIC
Sbjct: 669 KVISPGEECEKVFTALCQGKMIDSILECLKEWNGAPIPIC 708

BLAST of Spg013422 vs. ExPASy TrEMBL
Match: A0A6J1HGG8 (Phenylalanine ammonia-lyase OS=Cucurbita moschata OX=3662 GN=LOC111463284 PE=3 SV=1)

HSP 1 Score: 1340.9 bits (3469), Expect = 0.0e+00
Identity = 677/716 (94.55%), Postives = 699/716 (97.63%), Query Frame = 0

Query: 1   MAPAHFQQLACQNGSIESFCISQNDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGG 60
           MAPA FQQLA QNGS+E+FCI++NDPLNWGVAA++LKGSHLDEVKRMVEEYRRPLVKLGG
Sbjct: 1   MAPAPFQQLAAQNGSVETFCITRNDPLNWGVAADALKGSHLDEVKRMVEEYRRPLVKLGG 60

Query: 61  ETLTISQVAAIATRDSDVTVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR 120
           ETLTISQVAAIATRD+DV V+L+ESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR
Sbjct: 61  ETLTISQVAAIATRDNDVLVQLAESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR 120

Query: 121 RTKQGGALQKELIRFLNAGIFGSGAESNHTLPHSATRAAMLVRINTLLQGYSGIRFEILE 180
           RT QGGALQKELIRFLNAGIFGSGAES HTLPHSATRAAMLVRINTLLQGYSGIRFEILE
Sbjct: 121 RTNQGGALQKELIRFLNAGIFGSGAESTHTLPHSATRAAMLVRINTLLQGYSGIRFEILE 180

Query: 181 AITKLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGESIDAKEAFQQ 240
           AITKLLN NITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKA+GPNGES+DA EAFQQ
Sbjct: 181 AITKLLNSNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAVGPNGESLDAMEAFQQ 240

Query: 241 AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEF 300
           AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSE+LSAIFAEVMQGKPEF
Sbjct: 241 AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEF 300

Query: 301 TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWL 360
           TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMK AKKLHE DPLQKPKQDRYALRTSPQWL
Sbjct: 301 TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKAAKKLHETDPLQKPKQDRYALRTSPQWL 360

Query: 361 GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIG 420
           GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKA+HGGNFQGTPIGVSMDNTRLA+ASIG
Sbjct: 361 GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKAIHGGNFQGTPIGVSMDNTRLAVASIG 420

Query: 421 KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV 480
           KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV
Sbjct: 421 KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV 480

Query: 481 QSAEQHNQDVNSLGLISSRKTVEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTV 540
           QSAEQHNQDVNSLGLISSRKT EA+DILKLMSSTFLVALCQAIDLRHLEENLK+TVK+ V
Sbjct: 481 QSAEQHNQDVNSLGLISSRKTAEALDILKLMSSTFLVALCQAIDLRHLEENLKNTVKNCV 540

Query: 541 SQIAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLSQKLRQVLVEH 600
           SQ AKKVLTTSSNGALHPSRFCEKDLLKVVDRE+TFAYIDDPCSATYPLSQKLRQVLVEH
Sbjct: 541 SQTAKKVLTTSSNGALHPSRFCEKDLLKVVDREHTFAYIDDPCSATYPLSQKLRQVLVEH 600

Query: 601 ALANGEGEKNTDTSIFQKIIAFEEELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPL 660
           AL NG+ EK+ DTSIFQKI AFEEELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPL
Sbjct: 601 ALGNGDCEKDADTSIFQKIGAFEEELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPL 660

Query: 661 YRFVREELGTKLLTGEKVISPGEECEKVFTALCQGKMIDSILECLKEWNGAPIPIC 717
           YRFVREELGT L+TGEKVISPGEECEKVFTALC+GKMIDSILECLKEWNGAPIPIC
Sbjct: 661 YRFVREELGTNLVTGEKVISPGEECEKVFTALCEGKMIDSILECLKEWNGAPIPIC 716

BLAST of Spg013422 vs. ExPASy TrEMBL
Match: A0A6J1HG90 (Phenylalanine ammonia-lyase OS=Cucurbita moschata OX=3662 GN=LOC111463286 PE=3 SV=1)

HSP 1 Score: 1340.5 bits (3468), Expect = 0.0e+00
Identity = 677/716 (94.55%), Postives = 699/716 (97.63%), Query Frame = 0

Query: 1   MAPAHFQQLACQNGSIESFCISQNDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGG 60
           MAPA FQQLA QNGS+E+FCI++NDPLNWGVAA++LKGSHLDEVKRMVEEYRRPLVKLGG
Sbjct: 1   MAPAPFQQLAAQNGSVETFCITRNDPLNWGVAADALKGSHLDEVKRMVEEYRRPLVKLGG 60

Query: 61  ETLTISQVAAIATRDSDVTVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR 120
           ETLTISQVAAIATRD+DV V+L+ESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR
Sbjct: 61  ETLTISQVAAIATRDNDVLVQLAESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR 120

Query: 121 RTKQGGALQKELIRFLNAGIFGSGAESNHTLPHSATRAAMLVRINTLLQGYSGIRFEILE 180
           RT QGGALQKELIRFLNAGIFGSGAES HTLPHSATRAAMLVRINTLLQGYSGIRFEILE
Sbjct: 121 RTNQGGALQKELIRFLNAGIFGSGAESTHTLPHSATRAAMLVRINTLLQGYSGIRFEILE 180

Query: 181 AITKLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGESIDAKEAFQQ 240
           AITKLLN NITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKA+GPNGES+DA EAFQQ
Sbjct: 181 AITKLLNTNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAVGPNGESLDAMEAFQQ 240

Query: 241 AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEF 300
           AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSE+LSAIFAEVMQGKPEF
Sbjct: 241 AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEF 300

Query: 301 TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWL 360
           TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMK AKKLHE DPLQKPKQDRYALRTSPQWL
Sbjct: 301 TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKAAKKLHETDPLQKPKQDRYALRTSPQWL 360

Query: 361 GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIG 420
           GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKA+HGGNFQGTPIGVSMDNTRLA+ASIG
Sbjct: 361 GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKAIHGGNFQGTPIGVSMDNTRLAVASIG 420

Query: 421 KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV 480
           KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV
Sbjct: 421 KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV 480

Query: 481 QSAEQHNQDVNSLGLISSRKTVEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTV 540
           QSAEQHNQDVNSLGLISSRKT EA+DILKLMSSTFLVALCQAIDLRHLEENLK+TVK+ V
Sbjct: 481 QSAEQHNQDVNSLGLISSRKTAEALDILKLMSSTFLVALCQAIDLRHLEENLKNTVKNCV 540

Query: 541 SQIAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLSQKLRQVLVEH 600
           SQ AKKVLTTSSNGALHPSRFCEKDLLKVVDRE+TFAYIDDPCSATYPLSQKLRQVLVEH
Sbjct: 541 SQTAKKVLTTSSNGALHPSRFCEKDLLKVVDREHTFAYIDDPCSATYPLSQKLRQVLVEH 600

Query: 601 ALANGEGEKNTDTSIFQKIIAFEEELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPL 660
           AL NG+ EK+ DTSIFQKI AFEEELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPL
Sbjct: 601 ALGNGDCEKDADTSIFQKIGAFEEELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPL 660

Query: 661 YRFVREELGTKLLTGEKVISPGEECEKVFTALCQGKMIDSILECLKEWNGAPIPIC 717
           YRFVREELGT L+TGEKVISPGEECEKVFTALC+GKMIDSILECLKEWNGAPIPIC
Sbjct: 661 YRFVREELGTNLVTGEKVISPGEECEKVFTALCEGKMIDSILECLKEWNGAPIPIC 716

BLAST of Spg013422 vs. ExPASy TrEMBL
Match: A0A6J1HEN5 (Phenylalanine ammonia-lyase OS=Cucurbita moschata OX=3662 GN=LOC111463285 PE=3 SV=1)

HSP 1 Score: 1340.1 bits (3467), Expect = 0.0e+00
Identity = 677/716 (94.55%), Postives = 699/716 (97.63%), Query Frame = 0

Query: 1   MAPAHFQQLACQNGSIESFCISQNDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGG 60
           MAPA FQQLA QNGS+E+FCI++NDPLNWGVAA++LKGSHLDEVKRMVEEYRRPLVKLGG
Sbjct: 1   MAPAPFQQLAAQNGSVETFCITRNDPLNWGVAADALKGSHLDEVKRMVEEYRRPLVKLGG 60

Query: 61  ETLTISQVAAIATRDSDVTVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR 120
           ETLTISQVAAIATRD+DV V+L+ESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR
Sbjct: 61  ETLTISQVAAIATRDNDVLVQLAESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR 120

Query: 121 RTKQGGALQKELIRFLNAGIFGSGAESNHTLPHSATRAAMLVRINTLLQGYSGIRFEILE 180
           RT QGGALQKELIRFLNAGIFGSGAES HTLPHSATRAAMLVRINTLLQGYSGIRFEILE
Sbjct: 121 RTNQGGALQKELIRFLNAGIFGSGAESTHTLPHSATRAAMLVRINTLLQGYSGIRFEILE 180

Query: 181 AITKLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGESIDAKEAFQQ 240
           AITKLLN NITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKA+G NGES+DAKEAFQQ
Sbjct: 181 AITKLLNSNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAVGQNGESLDAKEAFQQ 240

Query: 241 AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEF 300
           AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSE+LSAIFAEVMQGKPEF
Sbjct: 241 AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEF 300

Query: 301 TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWL 360
           TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMK AKKLHE DPLQKPKQDRYALRTSPQWL
Sbjct: 301 TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKAAKKLHETDPLQKPKQDRYALRTSPQWL 360

Query: 361 GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIG 420
           GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKA+HGGNFQGTPIGVSMDNTRLA+ASIG
Sbjct: 361 GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKAIHGGNFQGTPIGVSMDNTRLAVASIG 420

Query: 421 KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV 480
           KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV
Sbjct: 421 KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV 480

Query: 481 QSAEQHNQDVNSLGLISSRKTVEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTV 540
           QSAEQHNQDVNSLGLISSRKT EA+DILKLMSSTFLVALCQAIDLRHLEENLK+TVK+ V
Sbjct: 481 QSAEQHNQDVNSLGLISSRKTAEALDILKLMSSTFLVALCQAIDLRHLEENLKNTVKNCV 540

Query: 541 SQIAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLSQKLRQVLVEH 600
           SQ AKKVLTTSSNGALHPSRFCEKDLLKVVDRE+TFAYIDDPCSATYPLSQKLRQVLVEH
Sbjct: 541 SQTAKKVLTTSSNGALHPSRFCEKDLLKVVDREHTFAYIDDPCSATYPLSQKLRQVLVEH 600

Query: 601 ALANGEGEKNTDTSIFQKIIAFEEELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPL 660
           AL NG+ EK+ DTSIFQKI AFEEELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPL
Sbjct: 601 ALGNGDCEKDADTSIFQKIGAFEEELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPL 660

Query: 661 YRFVREELGTKLLTGEKVISPGEECEKVFTALCQGKMIDSILECLKEWNGAPIPIC 717
           YRFVREELGT L+TGEKVISPGEECEKVFTALC+GKMIDSILECLKEWNGAPIPIC
Sbjct: 661 YRFVREELGTNLVTGEKVISPGEECEKVFTALCEGKMIDSILECLKEWNGAPIPIC 716

BLAST of Spg013422 vs. ExPASy TrEMBL
Match: A0A0A0KF39 (Phenylalanine ammonia-lyase OS=Cucumis sativus OX=3659 GN=Csa_6G445760 PE=3 SV=1)

HSP 1 Score: 1338.2 bits (3462), Expect = 0.0e+00
Identity = 682/716 (95.25%), Postives = 698/716 (97.49%), Query Frame = 0

Query: 1   MAPAHFQQLACQNGSIESFCISQNDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGG 60
           MA AHFQQ   +NGS+ES CI  NDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGG
Sbjct: 1   MAFAHFQQ---KNGSVESLCIGSNDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGG 60

Query: 61  ETLTISQVAAIATRDSDVTVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR 120
           ETLTISQVAAIATRD+DV VELSES+RAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR
Sbjct: 61  ETLTISQVAAIATRDTDVIVELSESSRAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR 120

Query: 121 RTKQGGALQKELIRFLNAGIFGSGAESNHTLPHSATRAAMLVRINTLLQGYSGIRFEILE 180
           RTKQGGALQKELIRFLNAGIFGSGAES+HTLPHSATRAAMLVRINTLLQGYSGIRFEILE
Sbjct: 121 RTKQGGALQKELIRFLNAGIFGSGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILE 180

Query: 181 AITKLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGESIDAKEAFQQ 240
           AITKLLN+NITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGE I AKEAFQQ
Sbjct: 181 AITKLLNNNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGERITAKEAFQQ 240

Query: 241 AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEF 300
           A IPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSE+LSAIFAEVMQGKPEF
Sbjct: 241 ADIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEF 300

Query: 301 TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWL 360
           TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWL
Sbjct: 301 TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWL 360

Query: 361 GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIG 420
           GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIG
Sbjct: 361 GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIG 420

Query: 421 KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV 480
           KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV
Sbjct: 421 KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV 480

Query: 481 QSAEQHNQDVNSLGLISSRKTVEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTV 540
           QSAEQHNQDVNSLGLISSRKT EAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVK+TV
Sbjct: 481 QSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKTTV 540

Query: 541 SQIAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLSQKLRQVLVEH 600
           SQIAKKVLTTSSNG LHPSRFCEKDLLKVVDREYTF+YIDDPCSATYPLSQKLRQVLVEH
Sbjct: 541 SQIAKKVLTTSSNGTLHPSRFCEKDLLKVVDREYTFSYIDDPCSATYPLSQKLRQVLVEH 600

Query: 601 ALANGEGEKNTDTSIFQKIIAFEEELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPL 660
           ALANG+ EK  DTSIFQKI +FEEELKAVLPKEVEN+RL+YESG+SKIENQIK+CRSYPL
Sbjct: 601 ALANGDNEKTVDTSIFQKIASFEEELKAVLPKEVENSRLAYESGSSKIENQIKNCRSYPL 660

Query: 661 YRFVREELGTKLLTGEKVISPGEECEKVFTALCQGKMIDSILECLKEWNGAPIPIC 717
           YRFVREEL TK+LTGEKVISPGEECEKVFTALCQGKMIDSILECLKEWNGAPIPIC
Sbjct: 661 YRFVREELETKMLTGEKVISPGEECEKVFTALCQGKMIDSILECLKEWNGAPIPIC 713

BLAST of Spg013422 vs. TAIR 10
Match: AT2G37040.1 (PHE ammonia lyase 1 )

HSP 1 Score: 1183.3 bits (3060), Expect = 0.0e+00
Identity = 590/700 (84.29%), Postives = 646/700 (92.29%), Query Frame = 0

Query: 17  ESFCISQNDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAIATRDS 76
           ++  I+  DPLNWG AAE +KGSHLDEVKRMV E+R+P+V LGGETLTI QVAAI+T  +
Sbjct: 26  KNMVINAEDPLNWGAAAEQMKGSHLDEVKRMVAEFRKPVVNLGGETLTIGQVAAISTIGN 85

Query: 77  DVTVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFL 136
            V VELSE+ARAGV ASSDWVMESMNKGTDSYGVTTGFGATSHRRTK G ALQKELIRFL
Sbjct: 86  SVKVELSETARAGVNASSDWVMESMNKGTDSYGVTTGFGATSHRRTKNGVALQKELIRFL 145

Query: 137 NAGIFGSGAESNHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCLPL 196
           NAGIFGS  E++HTLPHSATRAAMLVRINTLLQG+SGIRFEILEAIT  LN+NITP LPL
Sbjct: 146 NAGIFGSTKETSHTLPHSATRAAMLVRINTLLQGFSGIRFEILEAITSFLNNNITPSLPL 205

Query: 197 RGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGESIDAKEAFQQAGIPSGFFELQPKEGL 256
           RGTITASGDLVPLSYIAGLLTGRPNSKA GPNGE++ A+EAF+ AGI SGFF+LQPKEGL
Sbjct: 206 RGTITASGDLVPLSYIAGLLTGRPNSKATGPNGEALTAEEAFKLAGISSGFFDLQPKEGL 265

Query: 257 ALVNGTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEFTDHLTHKLKHHPGQIE 316
           ALVNGTAVGSG+AS+VLFE N+L+VL+E+LSA+FAEVM GKPEFTDHLTH+LKHHPGQIE
Sbjct: 266 ALVNGTAVGSGMASMVLFETNVLSVLAEILSAVFAEVMSGKPEFTDHLTHRLKHHPGQIE 325

Query: 317 AAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIER 376
           AAAIMEHILDGSSYMK A+KLHE+DPLQKPKQDRYALRTSPQWLGP IEVIR++TKSIER
Sbjct: 326 AAAIMEHILDGSSYMKLAQKLHEMDPLQKPKQDRYALRTSPQWLGPQIEVIRYATKSIER 385

Query: 377 EINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYN 436
           EINSVNDNPLIDVSRNKA+HGGNFQGTPIGVSMDNTRLAIA+IGKLMFAQFSELVNDFYN
Sbjct: 386 EINSVNDNPLIDVSRNKAIHGGNFQGTPIGVSMDNTRLAIAAIGKLMFAQFSELVNDFYN 445

Query: 437 NGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLI 496
           NGLPSNL+ASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLI
Sbjct: 446 NGLPSNLTASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLI 505

Query: 497 SSRKTVEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQIAKKVLTTSSNGAL 556
           SSRKT EA+DILKLMS+TFLVA+CQA+DLRHLEENL+ TVK+TVSQ+AKKVLTT  NG L
Sbjct: 506 SSRKTSEAVDILKLMSTTFLVAICQAVDLRHLEENLRQTVKNTVSQVAKKVLTTGVNGEL 565

Query: 557 HPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLSQKLRQVLVEHALANGEGEKNTDTSIF 616
           HPSRFCEKDLLKVVDRE  + Y DDPCSATYPL QKLRQV+V+HAL NGE EKN  TSIF
Sbjct: 566 HPSRFCEKDLLKVVDREQVYTYADDPCSATYPLIQKLRQVIVDHALINGESEKNAVTSIF 625

Query: 617 QKIIAFEEELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPLYRFVREELGTKLLTGE 676
            KI AFEEELKAVLPKEVE AR +Y++G S I N+IK+CRSYPLYRFVREELGT+LLTGE
Sbjct: 626 HKIGAFEEELKAVLPKEVEAARAAYDNGTSAIPNRIKECRSYPLYRFVREELGTELLTGE 685

Query: 677 KVISPGEECEKVFTALCQGKMIDSILECLKEWNGAPIPIC 717
           KV SPGEE +KVFTA+C+GK+ID ++ECL EWNGAPIPIC
Sbjct: 686 KVTSPGEEFDKVFTAICEGKIIDPMMECLNEWNGAPIPIC 725

BLAST of Spg013422 vs. TAIR 10
Match: AT3G53260.1 (phenylalanine ammonia-lyase 2 )

HSP 1 Score: 1180.6 bits (3053), Expect = 0.0e+00
Identity = 588/692 (84.97%), Postives = 644/692 (93.06%), Query Frame = 0

Query: 25  DPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAIATRDSDVTVELSE 84
           DPLNWG+AA+ +KGSHLDEVK+MVEEYRRP+V LGGETLTI QVAAI+T    V VEL+E
Sbjct: 26  DPLNWGLAADQMKGSHLDEVKKMVEEYRRPVVNLGGETLTIGQVAAISTVGGSVKVELAE 85

Query: 85  SARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFGSG 144
           ++RAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTK G ALQ ELIRFLNAGIFG+ 
Sbjct: 86  TSRAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKNGTALQTELIRFLNAGIFGNT 145

Query: 145 AESNHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCLPLRGTITASG 204
            E+ HTLP SATRAAMLVR+NTLLQGYSGIRFEILEAIT LLNHNI+P LPLRGTITASG
Sbjct: 146 KETCHTLPQSATRAAMLVRVNTLLQGYSGIRFEILEAITSLLNHNISPSLPLRGTITASG 205

Query: 205 DLVPLSYIAGLLTGRPNSKAIGPNGESIDAKEAFQQAGIPSGFFELQPKEGLALVNGTAV 264
           DLVPLSYIAGLLTGRPNSKA GP+GES+ AKEAF++AGI +GFF+LQPKEGLALVNGTAV
Sbjct: 206 DLVPLSYIAGLLTGRPNSKATGPDGESLTAKEAFEKAGISTGFFDLQPKEGLALVNGTAV 265

Query: 265 GSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEHI 324
           GSG+AS+VLFEAN+ AVL+EVLSAIFAEVM GKPEFTDHLTH+LKHHPGQIEAAAIMEHI
Sbjct: 266 GSGMASMVLFEANVQAVLAEVLSAIFAEVMSGKPEFTDHLTHRLKHHPGQIEAAAIMEHI 325

Query: 325 LDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIEREINSVNDN 384
           LDGSSYMK A+K+HE+DPLQKPKQDRYALRTSPQWLGP IEVIR +TKSIEREINSVNDN
Sbjct: 326 LDGSSYMKLAQKVHEMDPLQKPKQDRYALRTSPQWLGPQIEVIRQATKSIEREINSVNDN 385

Query: 385 PLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYNNGLPSNLS 444
           PLIDVSRNKA+HGGNFQGTPIGVSMDNTRLAIA+IGKLMFAQFSELVNDFYNNGLPSNL+
Sbjct: 386 PLIDVSRNKAIHGGNFQGTPIGVSMDNTRLAIAAIGKLMFAQFSELVNDFYNNGLPSNLT 445

Query: 445 ASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTVEA 504
           AS NPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKT EA
Sbjct: 446 ASSNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTSEA 505

Query: 505 IDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQIAKKVLTTSSNGALHPSRFCEK 564
           +DILKLMS+TFLV +CQA+DLRHLEENL+ TVK+TVSQ+AKKVLTT  NG LHPSRFCEK
Sbjct: 506 VDILKLMSTTFLVGICQAVDLRHLEENLRQTVKNTVSQVAKKVLTTGINGELHPSRFCEK 565

Query: 565 DLLKVVDREYTFAYIDDPCSATYPLSQKLRQVLVEHALANGEGEKNTDTSIFQKIIAFEE 624
           DLLKVVDRE  F Y+DDPCSATYPL Q+LRQV+V+HAL+NGE EKN  TSIFQKI AFEE
Sbjct: 566 DLLKVVDREQVFTYVDDPCSATYPLMQRLRQVIVDHALSNGETEKNAVTSIFQKIGAFEE 625

Query: 625 ELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPLYRFVREELGTKLLTGEKVISPGEE 684
           ELKAVLPKEVE AR +Y +G + I N+IK+CRSYPLYRFVREELGTKLLTGEKV+SPGEE
Sbjct: 626 ELKAVLPKEVEAARAAYGNGTAPIPNRIKECRSYPLYRFVREELGTKLLTGEKVVSPGEE 685

Query: 685 CEKVFTALCQGKMIDSILECLKEWNGAPIPIC 717
            +KVFTA+C+GK+ID +++CLKEWNGAPIPIC
Sbjct: 686 FDKVFTAMCEGKLIDPLMDCLKEWNGAPIPIC 717

BLAST of Spg013422 vs. TAIR 10
Match: AT3G10340.1 (phenylalanine ammonia-lyase 4 )

HSP 1 Score: 1130.2 bits (2922), Expect = 0.0e+00
Identity = 572/710 (80.56%), Postives = 629/710 (88.59%), Query Frame = 0

Query: 8   QLACQNGSIESFCISQNDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQ 67
           +L  QN  I +      DPLNW   AE+LKGSHLDEVKRMV+EYR+  VKLGGETLTI Q
Sbjct: 2   ELCNQNNHITAV---SGDPLNWNATAEALKGSHLDEVKRMVKEYRKEAVKLGGETLTIGQ 61

Query: 68  VAAIATRDSDVTVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGA 127
           VAA+A      TVEL+E ARAGVKASS+WVMESMN+GTDSYGVTTGFGATSHRRTKQGGA
Sbjct: 62  VAAVARGGGGSTVELAEEARAGVKASSEWVMESMNRGTDSYGVTTGFGATSHRRTKQGGA 121

Query: 128 LQKELIRFLNAGIFGSGA-ESNHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLL 187
           LQ ELIRFLNAGIFG GA +++HTLP   TRAAMLVR+NTLLQGYSGIRFEILEAITKLL
Sbjct: 122 LQNELIRFLNAGIFGPGAGDTSHTLPKPTTRAAMLVRVNTLLQGYSGIRFEILEAITKLL 181

Query: 188 NHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGESIDAKEAFQQAGIPSG 247
           NH ITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKA+GP+GE++ A EAF+ AG+ S 
Sbjct: 182 NHEITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAVGPSGETLTASEAFKLAGV-SS 241

Query: 248 FFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEFTDHLTH 307
           FFELQPKEGLALVNGTAVGSGLAS VLF+ANILAVLSEV+SA+FAEVMQGKPEFTDHLTH
Sbjct: 242 FFELQPKEGLALVNGTAVGSGLASTVLFDANILAVLSEVMSAMFAEVMQGKPEFTDHLTH 301

Query: 308 KLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEV 367
           KLKHHPGQIEAAAIMEHILDGSSY+K A+ LHE+DPLQKPKQDRYALRTSPQWLGP IEV
Sbjct: 302 KLKHHPGQIEAAAIMEHILDGSSYVKEAQLLHEMDPLQKPKQDRYALRTSPQWLGPQIEV 361

Query: 368 IRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQ 427
           IR +TK IEREINSVNDNPLIDVSRNKALHGGNFQGTPIGV+MDN+RLAIASIGKLMFAQ
Sbjct: 362 IRAATKMIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVAMDNSRLAIASIGKLMFAQ 421

Query: 428 FSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQH 487
           FSELVNDFYNNGLPSNLS  RNPSLDYGFKGAEIAMASYCSELQ+LANPVT+HVQSAEQH
Sbjct: 422 FSELVNDFYNNGLPSNLSGGRNPSLDYGFKGAEIAMASYCSELQFLANPVTNHVQSAEQH 481

Query: 488 NQDVNSLGLISSRKTVEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQIAKK 547
           NQDVNSLGLISSRKT EA+DILKLMS+T+LVALCQA+DLRHLEENLK  VKS VSQ+AK+
Sbjct: 482 NQDVNSLGLISSRKTAEAVDILKLMSTTYLVALCQAVDLRHLEENLKKAVKSAVSQVAKR 541

Query: 548 VLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLSQKLRQVLVEHALANGE 607
           VLT  +NG LHPSRF E+D+L+VVDREY F+Y DDPCS TYPL QKLR +LV+HALA+ E
Sbjct: 542 VLTVGANGELHPSRFTERDVLQVVDREYVFSYADDPCSLTYPLMQKLRHILVDHALADPE 601

Query: 608 GEKNTDTSIFQKIIAFEEELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPLYRFVRE 667
            E N+ TS+F KI AFE ELK +LPKEVE  R+ YE G S I N+IK+CRSYPLYRFVR+
Sbjct: 602 REANSATSVFHKIGAFEAELKLLLPKEVERVRVEYEEGTSAIANRIKECRSYPLYRFVRD 661

Query: 668 ELGTKLLTGEKVISPGEECEKVFTALCQGKMIDSILECLKEWNGAPIPIC 717
           EL T+LLTGE V SPGEE +KVF A+  GK+ID +LECLKEWNGAP+ IC
Sbjct: 662 ELNTELLTGENVRSPGEEFDKVFLAISDGKLIDPLLECLKEWNGAPVSIC 707

BLAST of Spg013422 vs. TAIR 10
Match: AT5G04230.1 (phenyl alanine ammonia-lyase 3 )

HSP 1 Score: 998.8 bits (2581), Expect = 2.2e-291
Identity = 521/696 (74.86%), Postives = 585/696 (84.05%), Query Frame = 0

Query: 24  NDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAIATRDSDVTVELS 83
           +DPLNW VAAE+LKGSHL+EVK+MV++YR+  V+LGGETLTI QVAA+A+     TVELS
Sbjct: 12  SDPLNWNVAAEALKGSHLEEVKKMVKDYRKGTVQLGGETLTIGQVAAVAS--GGPTVELS 71

Query: 84  ESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFGS 143
           E AR GVKASSDWVMESMN+ TD+YG+TTGFG++S RRT QG ALQKELIR+LNAGIF +
Sbjct: 72  EEARGGVKASSDWVMESMNRDTDTYGITTGFGSSSRRRTDQGAALQKELIRYLNAGIFAT 131

Query: 144 GAES---NHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCLPLRGTI 203
           G E    ++TLP  ATRAAML+R+NTLLQGYSGIRFEILEAIT LLN  ITP LPLRGTI
Sbjct: 132 GNEDDDRSNTLPRPATRAAMLIRVNTLLQGYSGIRFEILEAITTLLNCKITPLLPLRGTI 191

Query: 204 TASGDLVPLSYIAGLLTGRPNSKAIGPNGESIDAKEAFQQAGIPSGFFELQPKEGLALVN 263
           TASGDLVPLSYIAG L GRPNS+++GP+GE + A EAF+ AG+ S FFEL+PKEGLALVN
Sbjct: 192 TASGDLVPLSYIAGFLIGRPNSRSVGPSGEILTALEAFKLAGV-SSFFELRPKEGLALVN 251

Query: 264 GTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAI 323
           GTAVGS LAS VL++ANIL V SEV SA+FAEVMQGKPEFTDHLTHKLKHHPGQIEAAAI
Sbjct: 252 GTAVGSALASTVLYDANILVVFSEVASAMFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAI 311

Query: 324 MEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIEREINS 383
           MEHILDGSSY+K A  LH+IDPLQKPKQDRYALRTSPQWLGP IEVIR +TK IEREINS
Sbjct: 312 MEHILDGSSYVKEALHLHKIDPLQKPKQDRYALRTSPQWLGPQIEVIRAATKMIEREINS 371

Query: 384 VNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYNNGLP 443
           VNDNPLIDVSRNKA+HGGNFQGTPIGV+MDNTRLA+ASIGKLMFAQF+ELVNDFYNNGLP
Sbjct: 372 VNDNPLIDVSRNKAIHGGNFQGTPIGVAMDNTRLALASIGKLMFAQFTELVNDFYNNGLP 431

Query: 444 SNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRK 503
           SNLS  RNPSLDYG KGAE+AMASYCSELQ+LANPVT+HV+SA QHNQDVNSLGLISSR 
Sbjct: 432 SNLSGGRNPSLDYGLKGAEVAMASYCSELQFLANPVTNHVESASQHNQDVNSLGLISSRT 491

Query: 504 TVEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQIAKKVLTTSSNGALHPSR 563
           T EA+ ILKLMS+T+LVALCQA DLRHLEE LK  V   VS  AK VL      A+ P R
Sbjct: 492 TAEAVVILKLMSTTYLVALCQAFDLRHLEEILKKAVNEVVSHTAKSVL------AIEPFR 551

Query: 564 FCEKDLLKVVDREYTFAYIDDPCSATYPLSQKLRQVLVEHALANGEGEKNTDTSIFQKII 623
               D+L VV+REY F+Y+DDP S T PL QKLR VL + ALA  EGE  TDT +F+KI 
Sbjct: 552 -KHDDILGVVNREYVFSYVDDPSSLTNPLMQKLRHVLFDKALAEPEGE--TDT-VFRKIG 611

Query: 624 AFEEELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPLYRFVREELGTKLLTGEKVIS 683
           AFE ELK +LPKEVE  R  YE+G   + N+IK CRSYPLYRFVR EL T+LLTGE V S
Sbjct: 612 AFEAELKFLLPKEVERVRTEYENGTFNVANRIKKCRSYPLYRFVRNELETRLLTGEDVRS 671

Query: 684 PGEECEKVFTALCQGKMIDSILECLKEWNGAPIPIC 717
           PGE+ +KVF A+ QGK+ID + ECLKEWNGAPI IC
Sbjct: 672 PGEDFDKVFRAISQGKLIDPLFECLKEWNGAPISIC 694

BLAST of Spg013422 vs. TAIR 10
Match: AT5G04230.2 (phenyl alanine ammonia-lyase 3 )

HSP 1 Score: 993.0 bits (2566), Expect = 1.2e-289
Identity = 521/700 (74.43%), Postives = 585/700 (83.57%), Query Frame = 0

Query: 24  NDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAIATRDSDVTVELS 83
           +DPLNW VAAE+LKGSHL+EVK+MV++YR+  V+LGGETLTI QVAA+A+     TVELS
Sbjct: 12  SDPLNWNVAAEALKGSHLEEVKKMVKDYRKGTVQLGGETLTIGQVAAVAS--GGPTVELS 71

Query: 84  ESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFGS 143
           E AR GVKASSDWVMESMN+ TD+YG+TTGFG++S RRT QG ALQKELIR+LNAGIF +
Sbjct: 72  EEARGGVKASSDWVMESMNRDTDTYGITTGFGSSSRRRTDQGAALQKELIRYLNAGIFAT 131

Query: 144 GAES---NHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCLPLRGTI 203
           G E    ++TLP  ATRAAML+R+NTLLQGYSGIRFEILEAIT LLN  ITP LPLRGTI
Sbjct: 132 GNEDDDRSNTLPRPATRAAMLIRVNTLLQGYSGIRFEILEAITTLLNCKITPLLPLRGTI 191

Query: 204 TASGDLVPLSYIAGLLTGRPNSKAIGPNGESIDAKEAFQQAGIPSGFFELQPKEGLALVN 263
           TASGDLVPLSYIAG L GRPNS+++GP+GE + A EAF+ AG+ S FFEL+PKEGLALVN
Sbjct: 192 TASGDLVPLSYIAGFLIGRPNSRSVGPSGEILTALEAFKLAGV-SSFFELRPKEGLALVN 251

Query: 264 GTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAI 323
           GTAVGS LAS VL++ANIL V SEV SA+FAEVMQGKPEFTDHLTHKLKHHPGQIEAAAI
Sbjct: 252 GTAVGSALASTVLYDANILVVFSEVASAMFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAI 311

Query: 324 MEHILDGSSYMKTAKKLHEIDPLQKPKQDR----YALRTSPQWLGPLIEVIRFSTKSIER 383
           MEHILDGSSY+K A  LH+IDPLQKPKQDR    YALRTSPQWLGP IEVIR +TK IER
Sbjct: 312 MEHILDGSSYVKEALHLHKIDPLQKPKQDRYVLGYALRTSPQWLGPQIEVIRAATKMIER 371

Query: 384 EINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYN 443
           EINSVNDNPLIDVSRNKA+HGGNFQGTPIGV+MDNTRLA+ASIGKLMFAQF+ELVNDFYN
Sbjct: 372 EINSVNDNPLIDVSRNKAIHGGNFQGTPIGVAMDNTRLALASIGKLMFAQFTELVNDFYN 431

Query: 444 NGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLI 503
           NGLPSNLS  RNPSLDYG KGAE+AMASYCSELQ+LANPVT+HV+SA QHNQDVNSLGLI
Sbjct: 432 NGLPSNLSGGRNPSLDYGLKGAEVAMASYCSELQFLANPVTNHVESASQHNQDVNSLGLI 491

Query: 504 SSRKTVEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQIAKKVLTTSSNGAL 563
           SSR T EA+ ILKLMS+T+LVALCQA DLRHLEE LK  V   VS  AK VL      A+
Sbjct: 492 SSRTTAEAVVILKLMSTTYLVALCQAFDLRHLEEILKKAVNEVVSHTAKSVL------AI 551

Query: 564 HPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLSQKLRQVLVEHALANGEGEKNTDTSIF 623
            P R    D+L VV+REY F+Y+DDP S T PL QKLR VL + ALA  EGE  TDT +F
Sbjct: 552 EPFR-KHDDILGVVNREYVFSYVDDPSSLTNPLMQKLRHVLFDKALAEPEGE--TDT-VF 611

Query: 624 QKIIAFEEELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPLYRFVREELGTKLLTGE 683
           +KI AFE ELK +LPKEVE  R  YE+G   + N+IK CRSYPLYRFVR EL T+LLTGE
Sbjct: 612 RKIGAFEAELKFLLPKEVERVRTEYENGTFNVANRIKKCRSYPLYRFVRNELETRLLTGE 671

Query: 684 KVISPGEECEKVFTALCQGKMIDSILECLKEWNGAPIPIC 717
            V SPGE+ +KVF A+ QGK+ID + ECLKEWNGAPI IC
Sbjct: 672 DVRSPGEDFDKVFRAISQGKLIDPLFECLKEWNGAPISIC 698

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
APO14269.10.0e+0099.00phenylalanine ammonia-lyase 3 [Luffa aegyptiaca][more]
XP_038881628.10.0e+0095.25phenylalanine ammonia-lyase-like [Benincasa hispida][more]
XP_023518835.10.0e+0095.11phenylalanine ammonia-lyase-like isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_023518834.10.0e+0094.97phenylalanine ammonia-lyase-like isoform X1 [Cucurbita pepo subsp. pepo][more]
KAG6595242.10.0e+0094.97Phenylalanine ammonia-lyase, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
P457300.0e+0086.69Phenylalanine ammonia-lyase OS=Populus trichocarpa OX=3694 GN=PAL PE=2 SV=1[more]
P457320.0e+0086.26Phenylalanine ammonia-lyase OS=Stylosanthes humilis OX=35628 GN=PAL17.1 PE=2 SV=... [more]
P457340.0e+0087.79Phenylalanine ammonia-lyase OS=Trifolium subterraneum OX=3900 GN=PAL1 PE=3 SV=1[more]
Q045930.0e+0085.27Phenylalanine ammonia-lyase 2 OS=Pisum sativum OX=3888 GN=PAL2 PE=2 SV=1[more]
P279900.0e+0085.47Phenylalanine ammonia-lyase OS=Medicago sativa OX=3879 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1L5JHV30.0e+0099.00Phenylalanine ammonia-lyase OS=Luffa aegyptiaca OX=3670 PE=2 SV=1[more]
A0A6J1HGG80.0e+0094.55Phenylalanine ammonia-lyase OS=Cucurbita moschata OX=3662 GN=LOC111463284 PE=3 S... [more]
A0A6J1HG900.0e+0094.55Phenylalanine ammonia-lyase OS=Cucurbita moschata OX=3662 GN=LOC111463286 PE=3 S... [more]
A0A6J1HEN50.0e+0094.55Phenylalanine ammonia-lyase OS=Cucurbita moschata OX=3662 GN=LOC111463285 PE=3 S... [more]
A0A0A0KF390.0e+0095.25Phenylalanine ammonia-lyase OS=Cucumis sativus OX=3659 GN=Csa_6G445760 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G37040.10.0e+0084.29PHE ammonia lyase 1 [more]
AT3G53260.10.0e+0084.97phenylalanine ammonia-lyase 2 [more]
AT3G10340.10.0e+0080.56phenylalanine ammonia-lyase 4 [more]
AT5G04230.12.2e-29174.86phenyl alanine ammonia-lyase 3 [more]
AT5G04230.21.2e-28974.43phenyl alanine ammonia-lyase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.20.200.10Fumarase/aspartase (Central domain)coord: 263..711
e-value: 3.9E-243
score: 809.1
NoneNo IPR availablePANTHERPTHR10362:SF57PHENYLALANINE AMMONIA-LYASE 1-LIKEcoord: 22..716
IPR024083Fumarase/histidase, N-terminalGENE3D1.10.275.10coord: 17..262
e-value: 2.4E-99
score: 333.7
IPR005922Phenylalanine ammonia-lyaseTIGRFAMTIGR01226TIGR01226coord: 25..706
e-value: 0.0
score: 1173.5
IPR023144Phenylalanine ammonia-lyase, shielding domain superfamilyGENE3D1.10.274.20coord: 529..650
e-value: 3.9E-243
score: 809.1
IPR001106Aromatic amino acid lyasePFAMPF00221Lyase_aromaticcoord: 63..535
e-value: 2.0E-156
score: 521.2
IPR001106Aromatic amino acid lyasePANTHERPTHR10362HISTIDINE AMMONIA-LYASEcoord: 22..716
IPR001106Aromatic amino acid lyaseCDDcd00332PAL-HALcoord: 61..526
e-value: 0.0
score: 565.603
IPR022313Phenylalanine/histidine ammonia-lyases, active sitePROSITEPS00488PAL_HISTIDASEcoord: 198..214
IPR008948L-Aspartase-likeSUPERFAMILY48557L-aspartase-likecoord: 25..716

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg013422.1Spg013422.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009800 cinnamic acid biosynthetic process
biological_process GO:0006559 L-phenylalanine catabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0045548 phenylalanine ammonia-lyase activity
molecular_function GO:0016841 ammonia-lyase activity
molecular_function GO:0003824 catalytic activity