Spg013372 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg013372
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionmitochondrial uncoupling protein 5-like
Locationscaffold1: 7051230 .. 7052204 (-)
RNA-Seq ExpressionSpg013372
SyntenySpg013372
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTTTCAAGGGATTTCTTGAAGGAGGAATTGCTTCGATTGTTGCTGGGTGTTCGACCCACCCGCTTGATTTGATTAAGGTCCGAATGCAACTTCAGGGCGAGACTCAAGCTCCCGCGGCGGCGGTTCATAACCTCCGCCCGGCTCTCGCTTTTCAAACCACCTCTGTGACGGCTCCCAGGTCTATGAATATTCCTCCGCCGCCGCCTCCGGCGGCGCGTGTTGGCCCAGTCGCTGTCGGGATCCGTATTGTGCAGCAGGAAGGAGTTGCGGCTCTCTTCTCCGGCGTTTCCGCCACCGTTCTCCGGCAGACTATGTACTCGACTACCCGTATGGGGCTTTATGATATTCTCAAGCAGAAGTGGACCGATAAGGATACCGGAAAAATGCCGCTCGTTCGGAAGATCTCTGCCGGTTTGATCGCCGGTGCGGTCGGTGCTGCAGTCGGGAACCCGGCCGATGTTGCCATGGTTCGAATGCAAGCCGATGGGCGGCTGCCGCCGGCGCAGAGGCGGAACTACAAAAGCGTGGTCGACGCCATAACCCAGATGGCACGTGGCGAAGGCGTGACGAGCCTGTGGCGTGGTTCGTCGTTGACGGTGAACCGTGCAATGCTCGTAACGGCTTCGCAGCTTGCATCGTACGATCAGATTAAAGAGACGATTCTGCAGAAGGGATTGATGAAGGATGGGCTCGGGACCCACGTGACGGCGAGTTTCGCCGCGGGGTTTGTGGCGGCGGTGGCGTCGAACCCGGTTGACGTGATCAAAACGAGAGTGATGAACATGAAGGTGGAGCCCGGGGCGGCGGCGCCGTACTCCGGCGCGTTGGATTGCGCGTTGAAGACGGTACGGGCGGAGGGGCCGATGGCGCTGTACAAAGGGTTCATTCCGACGATTTCACGGCAGGGGCCGTTCACGGTGGTGCTGTTTGTGACGCTGGAACAAGTTCGAAAGGTGTTCAAAGATTTCTAA

mRNA sequence

ATGGGTTTCAAGGGATTTCTTGAAGGAGGAATTGCTTCGATTGTTGCTGGGTGTTCGACCCACCCGCTTGATTTGATTAAGGTCCGAATGCAACTTCAGGGCGAGACTCAAGCTCCCGCGGCGGCGGTTCATAACCTCCGCCCGGCTCTCGCTTTTCAAACCACCTCTGTGACGGCTCCCAGGTCTATGAATATTCCTCCGCCGCCGCCTCCGGCGGCGCGTGTTGGCCCAGTCGCTGTCGGGATCCGTATTGTGCAGCAGGAAGGAGTTGCGGCTCTCTTCTCCGGCGTTTCCGCCACCGTTCTCCGGCAGACTATGTACTCGACTACCCGTATGGGGCTTTATGATATTCTCAAGCAGAAGTGGACCGATAAGGATACCGGAAAAATGCCGCTCGTTCGGAAGATCTCTGCCGGTTTGATCGCCGGTGCGGTCGGTGCTGCAGTCGGGAACCCGGCCGATGTTGCCATGGTTCGAATGCAAGCCGATGGGCGGCTGCCGCCGGCGCAGAGGCGGAACTACAAAAGCGTGGTCGACGCCATAACCCAGATGGCACGTGGCGAAGGCGTGACGAGCCTGTGGCGTGGTTCGTCGTTGACGGTGAACCGTGCAATGCTCGTAACGGCTTCGCAGCTTGCATCGTACGATCAGATTAAAGAGACGATTCTGCAGAAGGGATTGATGAAGGATGGGCTCGGGACCCACGTGACGGCGAGTTTCGCCGCGGGGTTTGTGGCGGCGGTGGCGTCGAACCCGGTTGACGTGATCAAAACGAGAGTGATGAACATGAAGGTGGAGCCCGGGGCGGCGGCGCCGTACTCCGGCGCGTTGGATTGCGCGTTGAAGACGGTACGGGCGGAGGGGCCGATGGCGCTGTACAAAGGGTTCATTCCGACGATTTCACGGCAGGGGCCGTTCACGGTGGTGCTGTTTGTGACGCTGGAACAAGTTCGAAAGGTGTTCAAAGATTTCTAA

Coding sequence (CDS)

ATGGGTTTCAAGGGATTTCTTGAAGGAGGAATTGCTTCGATTGTTGCTGGGTGTTCGACCCACCCGCTTGATTTGATTAAGGTCCGAATGCAACTTCAGGGCGAGACTCAAGCTCCCGCGGCGGCGGTTCATAACCTCCGCCCGGCTCTCGCTTTTCAAACCACCTCTGTGACGGCTCCCAGGTCTATGAATATTCCTCCGCCGCCGCCTCCGGCGGCGCGTGTTGGCCCAGTCGCTGTCGGGATCCGTATTGTGCAGCAGGAAGGAGTTGCGGCTCTCTTCTCCGGCGTTTCCGCCACCGTTCTCCGGCAGACTATGTACTCGACTACCCGTATGGGGCTTTATGATATTCTCAAGCAGAAGTGGACCGATAAGGATACCGGAAAAATGCCGCTCGTTCGGAAGATCTCTGCCGGTTTGATCGCCGGTGCGGTCGGTGCTGCAGTCGGGAACCCGGCCGATGTTGCCATGGTTCGAATGCAAGCCGATGGGCGGCTGCCGCCGGCGCAGAGGCGGAACTACAAAAGCGTGGTCGACGCCATAACCCAGATGGCACGTGGCGAAGGCGTGACGAGCCTGTGGCGTGGTTCGTCGTTGACGGTGAACCGTGCAATGCTCGTAACGGCTTCGCAGCTTGCATCGTACGATCAGATTAAAGAGACGATTCTGCAGAAGGGATTGATGAAGGATGGGCTCGGGACCCACGTGACGGCGAGTTTCGCCGCGGGGTTTGTGGCGGCGGTGGCGTCGAACCCGGTTGACGTGATCAAAACGAGAGTGATGAACATGAAGGTGGAGCCCGGGGCGGCGGCGCCGTACTCCGGCGCGTTGGATTGCGCGTTGAAGACGGTACGGGCGGAGGGGCCGATGGCGCTGTACAAAGGGTTCATTCCGACGATTTCACGGCAGGGGCCGTTCACGGTGGTGCTGTTTGTGACGCTGGAACAAGTTCGAAAGGTGTTCAAAGATTTCTAA

Protein sequence

MGFKGFLEGGIASIVAGCSTHPLDLIKVRMQLQGETQAPAAAVHNLRPALAFQTTSVTAPRSMNIPPPPPPAARVGPVAVGIRIVQQEGVAALFSGVSATVLRQTMYSTTRMGLYDILKQKWTDKDTGKMPLVRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPPAQRRNYKSVVDAITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASFAAGFVAAVASNPVDVIKTRVMNMKVEPGAAAPYSGALDCALKTVRAEGPMALYKGFIPTISRQGPFTVVLFVTLEQVRKVFKDF
Homology
BLAST of Spg013372 vs. NCBI nr
Match: XP_008440559.1 (PREDICTED: mitochondrial uncoupling protein 5-like [Cucumis melo] >KAA0036336.1 mitochondrial uncoupling protein 5-like [Cucumis melo var. makuwa] >TYK12730.1 mitochondrial uncoupling protein 5-like [Cucumis melo var. makuwa])

HSP 1 Score: 596.3 bits (1536), Expect = 1.6e-166
Identity = 309/324 (95.37%), Postives = 317/324 (97.84%), Query Frame = 0

Query: 1   MGFKGFLEGGIASIVAGCSTHPLDLIKVRMQLQGETQAPAAAVHNLRPALAFQTTSVTAP 60
           MG KGF+EGGIASIVAGCSTHPLDLIKVRMQLQGET AP AAV+NLRPALAFQTTSVTAP
Sbjct: 1   MGVKGFVEGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTAAVNNLRPALAFQTTSVTAP 60

Query: 61  RSMNIPPPPPPAARVGPVAVGIRIVQQEGVAALFSGVSATVLRQTMYSTTRMGLYDILKQ 120
           RS+NIPPPPPP ARVGP+AVG+RIVQQEGVAALFSGVSATVLRQT+YSTTRMGLYDILKQ
Sbjct: 61  RSINIPPPPPPTARVGPIAVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDILKQ 120

Query: 121 KWTDKDTGKMPLVRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPPAQRRNYKSVVDA 180
           KWTD+DTGKMPL+RKISAGLIAGAVGAAVGNPADVAMVRMQADGRLP AQRRNYKSVVDA
Sbjct: 121 KWTDQDTGKMPLIRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPLAQRRNYKSVVDA 180

Query: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240
           ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF
Sbjct: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240

Query: 241 AAGFVAAVASNPVDVIKTRVMNMKVEPGAAAPYSGALDCALKTVRAEGPMALYKGFIPTI 300
           AAGFVAAVASNPVDVIKTRVMNMKVE GAA PYSGALDCALKTVRAEGPMALYKGFIPTI
Sbjct: 241 AAGFVAAVASNPVDVIKTRVMNMKVEAGAAPPYSGALDCALKTVRAEGPMALYKGFIPTI 300

Query: 301 SRQGPFTVVLFVTLEQVRKVFKDF 325
           SRQGPFTVVLFVTLEQVRKVFKDF
Sbjct: 301 SRQGPFTVVLFVTLEQVRKVFKDF 324

BLAST of Spg013372 vs. NCBI nr
Match: KAG6604059.1 (Mitochondrial uncoupling protein 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 595.1 bits (1533), Expect = 3.6e-166
Identity = 308/324 (95.06%), Postives = 316/324 (97.53%), Query Frame = 0

Query: 1   MGFKGFLEGGIASIVAGCSTHPLDLIKVRMQLQGETQAPAAAVHNLRPALAFQTTSVTAP 60
           MG KGF+EGGIASIVAGCSTHPLDLIKVRMQLQGET AP  AV NLRPALAFQTTSVTAP
Sbjct: 1   MGVKGFVEGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTGAVQNLRPALAFQTTSVTAP 60

Query: 61  RSMNIPPPPPPAARVGPVAVGIRIVQQEGVAALFSGVSATVLRQTMYSTTRMGLYDILKQ 120
           RSMNIP PPPPAARVGPVAVG+RIVQQEGVAALFSGVSATVLRQT+YSTTRMGLYD+LKQ
Sbjct: 61  RSMNIPSPPPPAARVGPVAVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDMLKQ 120

Query: 121 KWTDKDTGKMPLVRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPPAQRRNYKSVVDA 180
           KWTD+DTGK+PLVRKISAGLIAGA+GAAVGNPADVAMVRMQADGRLPPAQRRNYKSVVDA
Sbjct: 121 KWTDQDTGKLPLVRKISAGLIAGAIGAAVGNPADVAMVRMQADGRLPPAQRRNYKSVVDA 180

Query: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240
           ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF
Sbjct: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240

Query: 241 AAGFVAAVASNPVDVIKTRVMNMKVEPGAAAPYSGALDCALKTVRAEGPMALYKGFIPTI 300
           AAGFVAAVASNPVDVIKTRVMNMKVE GAAAPYSGALDCA+KTVRAEGPMALYKGFIPTI
Sbjct: 241 AAGFVAAVASNPVDVIKTRVMNMKVEAGAAAPYSGALDCAMKTVRAEGPMALYKGFIPTI 300

Query: 301 SRQGPFTVVLFVTLEQVRKVFKDF 325
           SRQGPFTVVLFVTLEQVRKV KDF
Sbjct: 301 SRQGPFTVVLFVTLEQVRKVLKDF 324

BLAST of Spg013372 vs. NCBI nr
Match: XP_038883604.1 (mitochondrial uncoupling protein 5-like [Benincasa hispida])

HSP 1 Score: 594.7 bits (1532), Expect = 4.7e-166
Identity = 309/324 (95.37%), Postives = 315/324 (97.22%), Query Frame = 0

Query: 1   MGFKGFLEGGIASIVAGCSTHPLDLIKVRMQLQGETQAPAAAVHNLRPALAFQTTSVTAP 60
           MG KGF+EGGIASIVAGCSTHPLDLIKVRMQLQGET AP AAVHNLRPALAFQTTSVTAP
Sbjct: 1   MGVKGFVEGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTAAVHNLRPALAFQTTSVTAP 60

Query: 61  RSMNIPPPPPPAARVGPVAVGIRIVQQEGVAALFSGVSATVLRQTMYSTTRMGLYDILKQ 120
           RSMNIPPPPPPAARVGP+ VG+RIVQQEGVAALFSGVSATVLRQT+YSTTRMGLYDILKQ
Sbjct: 61  RSMNIPPPPPPAARVGPITVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDILKQ 120

Query: 121 KWTDKDTGKMPLVRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPPAQRRNYKSVVDA 180
           KWTD+DTGKM LVRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLP AQRRNYKSVVDA
Sbjct: 121 KWTDQDTGKMSLVRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPVAQRRNYKSVVDA 180

Query: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240
           ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF
Sbjct: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240

Query: 241 AAGFVAAVASNPVDVIKTRVMNMKVEPGAAAPYSGALDCALKTVRAEGPMALYKGFIPTI 300
           AAGFVAAVASNPVDVIKTRVMNMKVE G+A PYSGALDCALKTVRAEGPMALYKGFIPTI
Sbjct: 241 AAGFVAAVASNPVDVIKTRVMNMKVEAGSAPPYSGALDCALKTVRAEGPMALYKGFIPTI 300

Query: 301 SRQGPFTVVLFVTLEQVRKVFKDF 325
           SRQGPFTVVLFVTLEQVRKV KDF
Sbjct: 301 SRQGPFTVVLFVTLEQVRKVLKDF 324

BLAST of Spg013372 vs. NCBI nr
Match: XP_004143448.1 (mitochondrial uncoupling protein 5 [Cucumis sativus] >KGN48714.1 hypothetical protein Csa_002838 [Cucumis sativus])

HSP 1 Score: 594.0 bits (1530), Expect = 8.0e-166
Identity = 307/324 (94.75%), Postives = 316/324 (97.53%), Query Frame = 0

Query: 1   MGFKGFLEGGIASIVAGCSTHPLDLIKVRMQLQGETQAPAAAVHNLRPALAFQTTSVTAP 60
           MG KGF+EGGIASIVAGCSTHPLDLIKVRMQLQGET AP AAVHNLRPALAFQTTSVTAP
Sbjct: 1   MGVKGFVEGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTAAVHNLRPALAFQTTSVTAP 60

Query: 61  RSMNIPPPPPPAARVGPVAVGIRIVQQEGVAALFSGVSATVLRQTMYSTTRMGLYDILKQ 120
           +S+NIPPPPPP ARVGP+AVG+RIVQQEGVAALFSGVSATVLRQT+YSTTRMGLYDILKQ
Sbjct: 61  KSINIPPPPPPPARVGPIAVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDILKQ 120

Query: 121 KWTDKDTGKMPLVRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPPAQRRNYKSVVDA 180
           KWTD+DTGKMPL+RKISAGLIAGAVGAAVGNPADVAMVRMQADGRLP AQRRNYKSVVDA
Sbjct: 121 KWTDQDTGKMPLIRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPLAQRRNYKSVVDA 180

Query: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240
           ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF
Sbjct: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240

Query: 241 AAGFVAAVASNPVDVIKTRVMNMKVEPGAAAPYSGALDCALKTVRAEGPMALYKGFIPTI 300
           AAGFVA+VASNPVDVIKTRVMNMKVE GAA PYSGALDCALKTVRAEGPMALYKGFIPTI
Sbjct: 241 AAGFVASVASNPVDVIKTRVMNMKVEAGAAPPYSGALDCALKTVRAEGPMALYKGFIPTI 300

Query: 301 SRQGPFTVVLFVTLEQVRKVFKDF 325
           SRQGPFTVVLFVTLEQVRKV KDF
Sbjct: 301 SRQGPFTVVLFVTLEQVRKVLKDF 324

BLAST of Spg013372 vs. NCBI nr
Match: XP_023544374.1 (mitochondrial uncoupling protein 5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 593.2 bits (1528), Expect = 1.4e-165
Identity = 307/324 (94.75%), Postives = 315/324 (97.22%), Query Frame = 0

Query: 1   MGFKGFLEGGIASIVAGCSTHPLDLIKVRMQLQGETQAPAAAVHNLRPALAFQTTSVTAP 60
           MG KGF+EGGIASIVAGCSTHPLDLIKVRMQLQGET AP  AV NLRPALAFQT SVTAP
Sbjct: 1   MGVKGFVEGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTGAVQNLRPALAFQTNSVTAP 60

Query: 61  RSMNIPPPPPPAARVGPVAVGIRIVQQEGVAALFSGVSATVLRQTMYSTTRMGLYDILKQ 120
           RSMNIP PPPPAARVGPVAVG+RIVQQEGVAALFSGVSATVLRQT+YSTTRMGLYD+LKQ
Sbjct: 61  RSMNIPSPPPPAARVGPVAVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDMLKQ 120

Query: 121 KWTDKDTGKMPLVRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPPAQRRNYKSVVDA 180
           KWTD+DTGK+PLVRKISAGLIAGA+GAAVGNPADVAMVRMQADGRLPPAQRRNYKSVVDA
Sbjct: 121 KWTDQDTGKLPLVRKISAGLIAGAIGAAVGNPADVAMVRMQADGRLPPAQRRNYKSVVDA 180

Query: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240
           ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF
Sbjct: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240

Query: 241 AAGFVAAVASNPVDVIKTRVMNMKVEPGAAAPYSGALDCALKTVRAEGPMALYKGFIPTI 300
           AAGFVAAVASNPVDVIKTRVMNMKVE GAAAPYSGALDCA+KTVRAEGPMALYKGFIPTI
Sbjct: 241 AAGFVAAVASNPVDVIKTRVMNMKVEAGAAAPYSGALDCAMKTVRAEGPMALYKGFIPTI 300

Query: 301 SRQGPFTVVLFVTLEQVRKVFKDF 325
           SRQGPFTVVLFVTLEQVRKV KDF
Sbjct: 301 SRQGPFTVVLFVTLEQVRKVLKDF 324

BLAST of Spg013372 vs. ExPASy Swiss-Prot
Match: Q9SJY5 (Mitochondrial uncoupling protein 5 OS=Arabidopsis thaliana OX=3702 GN=PUMP5 PE=2 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 7.1e-133
Identity = 248/324 (76.54%), Postives = 281/324 (86.73%), Query Frame = 0

Query: 1   MGFKGFLEGGIASIVAGCSTHPLDLIKVRMQLQGETQAPAAAVHNLRPALAFQTTSVTAP 60
           MG KGF EGGIASIVAGCSTHPLDLIKVRMQLQGE+   A    NLRPALAFQT++    
Sbjct: 1   MGLKGFAEGGIASIVAGCSTHPLDLIKVRMQLQGES---APIQTNLRPALAFQTST---- 60

Query: 61  RSMNIPPPPPPAARVGPVAVGIRIVQQEGVAALFSGVSATVLRQTMYSTTRMGLYDILKQ 120
            ++N PP      RVG + VG R++++EG+ ALFSGVSATVLRQT+YSTTRMGLYDI+K 
Sbjct: 61  -TVNAPP-----LRVGVIGVGSRLIREEGMRALFSGVSATVLRQTLYSTTRMGLYDIIKG 120

Query: 121 KWTDKDTGKMPLVRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPPAQRRNYKSVVDA 180
           +WTD +T  MPL++KI AG IAGA+GAAVGNPADVAMVRMQADGRLP   RRNYKSV+DA
Sbjct: 121 EWTDPETKTMPLMKKIGAGAIAGAIGAAVGNPADVAMVRMQADGRLPLTDRRNYKSVLDA 180

Query: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240
           ITQM RGEGVTSLWRGSSLT+NRAMLVT+SQLASYD +KETIL+KGL+KDGLGTHV+ASF
Sbjct: 181 ITQMIRGEGVTSLWRGSSLTINRAMLVTSSQLASYDSVKETILEKGLLKDGLGTHVSASF 240

Query: 241 AAGFVAAVASNPVDVIKTRVMNMKVEPGAAAPYSGALDCALKTVRAEGPMALYKGFIPTI 300
           AAGFVA+VASNPVDVIKTRVMNMKV  G A PY GA+DCALKTV+AEG M+LYKGFIPT+
Sbjct: 241 AAGFVASVASNPVDVIKTRVMNMKVVAGVAPPYKGAVDCALKTVKAEGIMSLYKGFIPTV 300

Query: 301 SRQGPFTVVLFVTLEQVRKVFKDF 325
           SRQ PFTVVLFVTLEQV+K+FKD+
Sbjct: 301 SRQAPFTVVLFVTLEQVKKLFKDY 311

BLAST of Spg013372 vs. ExPASy Swiss-Prot
Match: Q9SB52 (Mitochondrial uncoupling protein 4 OS=Arabidopsis thaliana OX=3702 GN=PUMP4 PE=2 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 4.8e-121
Identity = 229/324 (70.68%), Postives = 263/324 (81.17%), Query Frame = 0

Query: 1   MGFKGFLEGGIASIVAGCSTHPLDLIKVRMQLQGETQAPAAAVHNLRPALAFQTTSVTAP 60
           MG K F+EGGIAS++AGCSTHPLDLIKVR+QL GE  +    V  LRPALAF  +S  A 
Sbjct: 1   MGVKSFVEGGIASVIAGCSTHPLDLIKVRLQLHGEAPS-TTTVTLLRPALAFPNSSPAAF 60

Query: 61  RSMNIPPPPPPAARVGPVAVGIRIVQQEGVAALFSGVSATVLRQTMYSTTRMGLYDILKQ 120
                  P     +VGP+++GI IV+ EG AALFSGVSAT+LRQT+YSTTRMGLY++LK 
Sbjct: 61  LETTSSVP-----KVGPISLGINIVKSEGAAALFSGVSATLLRQTLYSTTRMGLYEVLKN 120

Query: 121 KWTDKDTGKMPLVRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPPAQRRNYKSVVDA 180
           KWTD ++GK+ L RKI AGL+AG +GAAVGNPADVAMVRMQADGRLP AQRRNY  V DA
Sbjct: 121 KWTDPESGKLNLSRKIGAGLVAGGIGAAVGNPADVAMVRMQADGRLPLAQRRNYAGVGDA 180

Query: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240
           I  M +GEGVTSLWRGS+LT+NRAM+VTA+QLASYDQ KE IL+ G+M DGLGTHV ASF
Sbjct: 181 IRSMVKGEGVTSLWRGSALTINRAMIVTAAQLASYDQFKEGILENGVMNDGLGTHVVASF 240

Query: 241 AAGFVAAVASNPVDVIKTRVMNMKVEPGAAAPYSGALDCALKTVRAEGPMALYKGFIPTI 300
           AAGFVA+VASNPVDVIKTRVMNMKV       Y GA DCA+KTV+AEG MALYKGF+PT+
Sbjct: 241 AAGFVASVASNPVDVIKTRVMNMKV-----GAYDGAWDCAVKTVKAEGAMALYKGFVPTV 300

Query: 301 SRQGPFTVVLFVTLEQVRKVFKDF 325
            RQGPFTVVLFVTLEQVRK+ +DF
Sbjct: 301 CRQGPFTVVLFVTLEQVRKLLRDF 313

BLAST of Spg013372 vs. ExPASy Swiss-Prot
Match: Q9FY68 (Mitochondrial uncoupling protein 6 OS=Arabidopsis thaliana OX=3702 GN=PUMP6 PE=2 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 5.5e-101
Identity = 211/339 (62.24%), Postives = 248/339 (73.16%), Query Frame = 0

Query: 1   MGFKGFLEGGIASIVAGCSTHPLDLIKVRMQLQGE-----TQAP---AAAVHNL-----R 60
           MGFK FLEGGIA+I+AG  THPLDLIKVRMQLQGE      Q P    +  HNL     R
Sbjct: 1   MGFKPFLEGGIAAIIAGALTHPLDLIKVRMQLQGEHSFSLDQNPNPNLSLDHNLPVKPYR 60

Query: 61  PALAFQTT--SVTAPRSMNIPPPPPPAARVGPVAVGIRIVQQEGVAALFSGVSATVLRQT 120
           P  A  +   S++        P     + + P AVG  IV+ EG AALFSGVSAT+LRQ 
Sbjct: 61  PVFALDSLIGSISLLPLHIHAPSSSTRSVMTPFAVGAHIVKTEGPAALFSGVSATILRQM 120

Query: 121 MYSTTRMGLYDILKQKWTDKDTGKMPLVRKISAGLIAGAVGAAVGNPADVAMVRMQADGR 180
           +YS TRMG+YD LK++WTD+ TG  PLV KI+AGLIAGAVG+ VGNPADVAMVRMQADG 
Sbjct: 121 LYSATRMGIYDFLKRRWTDQLTGNFPLVTKITAGLIAGAVGSVVGNPADVAMVRMQADGS 180

Query: 181 LPPAQRRNYKSVVDAITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQK 240
           LP  +RRNYKSVVDAI ++AR EGV+SLWRGS LTVNRAM+VTASQLA+YD +KE ++  
Sbjct: 181 LPLNRRRNYKSVVDAIDRIARQEGVSSLWRGSWLTVNRAMIVTASQLATYDHVKEILVAG 240

Query: 241 GL-MKDGLGTHVTASFAAGFVAAVASNPVDVIKTRVMNMKVEPGAAAPYSGALDCALKTV 300
           G     G+GTHV ASFAAG VAAVASNP+DV+KTR+MN   E      Y G LDCA+K V
Sbjct: 241 GRGTPGGIGTHVAASFAAGIVAAVASNPIDVVKTRMMNADKE-----IYGGPLDCAVKMV 300

Query: 301 RAEGPMALYKGFIPTISRQGPFTVVLFVTLEQVRKVFKD 324
             EGPMALYKG +PT +RQGPFT++LF+TLEQVR + KD
Sbjct: 301 AEEGPMALYKGLVPTATRQGPFTMILFLTLEQVRGLLKD 334

BLAST of Spg013372 vs. ExPASy Swiss-Prot
Match: Q54PY7 (Probable mitochondrial 2-oxoglutarate/malate carrier protein OS=Dictyostelium discoideum OX=44689 GN=ucpC PE=3 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 2.0e-63
Identity = 140/319 (43.89%), Postives = 191/319 (59.87%), Query Frame = 0

Query: 4   KGFLEGGIASIVAGCSTHPLDLIKVRMQLQGETQAPAAAVHNLRPALAFQTTSVTAPRSM 63
           K F+ GG+A +++   THP+D +KVRMQLQGE                   T V   R  
Sbjct: 26  KQFVIGGLAGMLSSAFTHPIDSLKVRMQLQGE------------------GTGVGPKR-- 85

Query: 64  NIPPPPPPAARVGPVAVGIRIVQQEGVAALFSGVSATVLRQTMYSTTRMGLYDILKQKWT 123
                       G + + + I Q EG   L+ G+SA++LRQ  Y+TTR GLYD++K    
Sbjct: 86  ------------GALKMLVHINQTEGFFTLYKGLSASLLRQATYTTTRFGLYDLIKD-IV 145

Query: 124 DKDTGKMPLVRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPPAQRRNYKSVVDAITQ 183
            KD   +P  +KI  G+++GA GA VG PAD+ MVRMQADG+LP   RRNYK+V D I +
Sbjct: 146 AKDDKPLPFTQKIMVGMLSGAGGAIVGTPADLTMVRMQADGKLPFNLRRNYKNVFDGIFR 205

Query: 184 MARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASFAAG 243
           +++ EG+ SLW+G S  + RAM +TA Q++SYDQ K+ +L  G   D + TH+ AS  A 
Sbjct: 206 ISKEEGIISLWKGCSPNLIRAMFMTAGQVSSYDQTKQLMLASGYFHDDIKTHLIASTTAA 265

Query: 244 FVAAVASNPVDVIKTRVMNMKVEPGAAAPYSGALDCALKTVRAEGPMALYKGFIPTISRQ 303
           FVAAVA++P+DVIKTR+MN          Y G  DC  KT+RAEG  A YKGF P   R 
Sbjct: 266 FVAAVATSPLDVIKTRIMNSPKTVTGELQYKGTFDCLSKTLRAEGFKAFYKGFNPYFMRL 311

Query: 304 GPFTVVLFVTLEQVRKVFK 323
           GP T++ F+ +EQ+  ++K
Sbjct: 326 GPQTILTFIFVEQLNILWK 311

BLAST of Spg013372 vs. ExPASy Swiss-Prot
Match: Q9CR62 (Mitochondrial 2-oxoglutarate/malate carrier protein OS=Mus musculus OX=10090 GN=Slc25a11 PE=1 SV=3)

HSP 1 Score: 221.5 bits (563), Expect = 1.4e-56
Identity = 130/317 (41.01%), Postives = 181/317 (57.10%), Query Frame = 0

Query: 6   FLEGGIASIVAGCSTHPLDLIKVRMQLQGETQAPAAAVHNLRPALAFQTTSVTAPRSMNI 65
           FL GG+A + A     PLDL+K RMQL GE     A     + +    T+          
Sbjct: 25  FLFGGLAGMGATVFVQPLDLVKNRMQLSGE----GAKTREYKTSFHALTS---------- 84

Query: 66  PPPPPPAARVGPVAVGIRIVQQEGVAALFSGVSATVLRQTMYSTTRMGLYDILKQKWTDK 125
                             I++ EG+  +++G+SA +LRQ  Y+TTR+G+Y +L ++ T  
Sbjct: 85  ------------------ILKTEGLKGIYTGLSAGLLRQATYTTTRLGIYTVLFERLTGA 144

Query: 126 DTGKMPLVRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPPAQRRNYKSVVDAITQMA 185
           D      + K   G+ AGA GA VG PA+VA++RM ADGRLP  QRR YK+V +A+ ++A
Sbjct: 145 DGTPPGFLLKALIGMTAGATGAFVGTPAEVALIRMTADGRLPADQRRGYKNVFNALVRIA 204

Query: 186 RGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASFAAGFV 245
           R EGV +LWRG   T+ RA++V A+QLASY Q K+ +L  G   D +  H  AS  +G V
Sbjct: 205 REEGVPTLWRGCIPTMARAVVVNAAQLASYSQSKQFLLDSGYFSDNILCHFCASMISGLV 264

Query: 246 AAVASNPVDVIKTRVMNMKVEPGAAAPYSGALDCALKTVRAEGPMALYKGFIPTISRQGP 305
              AS PVD++KTR+ NM++  G    Y   LD  LK VR EG  +L+KGF P  +R GP
Sbjct: 265 TTAASMPVDIVKTRIQNMRMIDG-KPEYKNGLDVLLKVVRYEGFFSLWKGFTPYYARLGP 308

Query: 306 FTVVLFVTLEQVRKVFK 323
            TV+ F+ LEQ+ K +K
Sbjct: 325 HTVLTFIFLEQMNKAYK 308

BLAST of Spg013372 vs. ExPASy TrEMBL
Match: A0A5A7T2E3 (Mitochondrial uncoupling protein 5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G003180 PE=3 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 7.8e-167
Identity = 309/324 (95.37%), Postives = 317/324 (97.84%), Query Frame = 0

Query: 1   MGFKGFLEGGIASIVAGCSTHPLDLIKVRMQLQGETQAPAAAVHNLRPALAFQTTSVTAP 60
           MG KGF+EGGIASIVAGCSTHPLDLIKVRMQLQGET AP AAV+NLRPALAFQTTSVTAP
Sbjct: 1   MGVKGFVEGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTAAVNNLRPALAFQTTSVTAP 60

Query: 61  RSMNIPPPPPPAARVGPVAVGIRIVQQEGVAALFSGVSATVLRQTMYSTTRMGLYDILKQ 120
           RS+NIPPPPPP ARVGP+AVG+RIVQQEGVAALFSGVSATVLRQT+YSTTRMGLYDILKQ
Sbjct: 61  RSINIPPPPPPTARVGPIAVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDILKQ 120

Query: 121 KWTDKDTGKMPLVRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPPAQRRNYKSVVDA 180
           KWTD+DTGKMPL+RKISAGLIAGAVGAAVGNPADVAMVRMQADGRLP AQRRNYKSVVDA
Sbjct: 121 KWTDQDTGKMPLIRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPLAQRRNYKSVVDA 180

Query: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240
           ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF
Sbjct: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240

Query: 241 AAGFVAAVASNPVDVIKTRVMNMKVEPGAAAPYSGALDCALKTVRAEGPMALYKGFIPTI 300
           AAGFVAAVASNPVDVIKTRVMNMKVE GAA PYSGALDCALKTVRAEGPMALYKGFIPTI
Sbjct: 241 AAGFVAAVASNPVDVIKTRVMNMKVEAGAAPPYSGALDCALKTVRAEGPMALYKGFIPTI 300

Query: 301 SRQGPFTVVLFVTLEQVRKVFKDF 325
           SRQGPFTVVLFVTLEQVRKVFKDF
Sbjct: 301 SRQGPFTVVLFVTLEQVRKVFKDF 324

BLAST of Spg013372 vs. ExPASy TrEMBL
Match: A0A1S3B1F2 (mitochondrial uncoupling protein 5-like OS=Cucumis melo OX=3656 GN=LOC103484939 PE=3 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 7.8e-167
Identity = 309/324 (95.37%), Postives = 317/324 (97.84%), Query Frame = 0

Query: 1   MGFKGFLEGGIASIVAGCSTHPLDLIKVRMQLQGETQAPAAAVHNLRPALAFQTTSVTAP 60
           MG KGF+EGGIASIVAGCSTHPLDLIKVRMQLQGET AP AAV+NLRPALAFQTTSVTAP
Sbjct: 1   MGVKGFVEGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTAAVNNLRPALAFQTTSVTAP 60

Query: 61  RSMNIPPPPPPAARVGPVAVGIRIVQQEGVAALFSGVSATVLRQTMYSTTRMGLYDILKQ 120
           RS+NIPPPPPP ARVGP+AVG+RIVQQEGVAALFSGVSATVLRQT+YSTTRMGLYDILKQ
Sbjct: 61  RSINIPPPPPPTARVGPIAVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDILKQ 120

Query: 121 KWTDKDTGKMPLVRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPPAQRRNYKSVVDA 180
           KWTD+DTGKMPL+RKISAGLIAGAVGAAVGNPADVAMVRMQADGRLP AQRRNYKSVVDA
Sbjct: 121 KWTDQDTGKMPLIRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPLAQRRNYKSVVDA 180

Query: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240
           ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF
Sbjct: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240

Query: 241 AAGFVAAVASNPVDVIKTRVMNMKVEPGAAAPYSGALDCALKTVRAEGPMALYKGFIPTI 300
           AAGFVAAVASNPVDVIKTRVMNMKVE GAA PYSGALDCALKTVRAEGPMALYKGFIPTI
Sbjct: 241 AAGFVAAVASNPVDVIKTRVMNMKVEAGAAPPYSGALDCALKTVRAEGPMALYKGFIPTI 300

Query: 301 SRQGPFTVVLFVTLEQVRKVFKDF 325
           SRQGPFTVVLFVTLEQVRKVFKDF
Sbjct: 301 SRQGPFTVVLFVTLEQVRKVFKDF 324

BLAST of Spg013372 vs. ExPASy TrEMBL
Match: A0A0A0KIS8 (Mitochondrial dicarboxylate carrier protein OS=Cucumis sativus OX=3659 GN=Csa_6G499090 PE=3 SV=1)

HSP 1 Score: 594.0 bits (1530), Expect = 3.9e-166
Identity = 307/324 (94.75%), Postives = 316/324 (97.53%), Query Frame = 0

Query: 1   MGFKGFLEGGIASIVAGCSTHPLDLIKVRMQLQGETQAPAAAVHNLRPALAFQTTSVTAP 60
           MG KGF+EGGIASIVAGCSTHPLDLIKVRMQLQGET AP AAVHNLRPALAFQTTSVTAP
Sbjct: 1   MGVKGFVEGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTAAVHNLRPALAFQTTSVTAP 60

Query: 61  RSMNIPPPPPPAARVGPVAVGIRIVQQEGVAALFSGVSATVLRQTMYSTTRMGLYDILKQ 120
           +S+NIPPPPPP ARVGP+AVG+RIVQQEGVAALFSGVSATVLRQT+YSTTRMGLYDILKQ
Sbjct: 61  KSINIPPPPPPPARVGPIAVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDILKQ 120

Query: 121 KWTDKDTGKMPLVRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPPAQRRNYKSVVDA 180
           KWTD+DTGKMPL+RKISAGLIAGAVGAAVGNPADVAMVRMQADGRLP AQRRNYKSVVDA
Sbjct: 121 KWTDQDTGKMPLIRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPLAQRRNYKSVVDA 180

Query: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240
           ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF
Sbjct: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240

Query: 241 AAGFVAAVASNPVDVIKTRVMNMKVEPGAAAPYSGALDCALKTVRAEGPMALYKGFIPTI 300
           AAGFVA+VASNPVDVIKTRVMNMKVE GAA PYSGALDCALKTVRAEGPMALYKGFIPTI
Sbjct: 241 AAGFVASVASNPVDVIKTRVMNMKVEAGAAPPYSGALDCALKTVRAEGPMALYKGFIPTI 300

Query: 301 SRQGPFTVVLFVTLEQVRKVFKDF 325
           SRQGPFTVVLFVTLEQVRKV KDF
Sbjct: 301 SRQGPFTVVLFVTLEQVRKVLKDF 324

BLAST of Spg013372 vs. ExPASy TrEMBL
Match: A0A6J1IN72 (mitochondrial uncoupling protein 5-like OS=Cucurbita maxima OX=3661 GN=LOC111477955 PE=3 SV=1)

HSP 1 Score: 591.7 bits (1524), Expect = 1.9e-165
Identity = 306/324 (94.44%), Postives = 315/324 (97.22%), Query Frame = 0

Query: 1   MGFKGFLEGGIASIVAGCSTHPLDLIKVRMQLQGETQAPAAAVHNLRPALAFQTTSVTAP 60
           MG KGF+EGGIASIVAGCSTHPLDLIKVRMQLQGET AP  AV NLRPALAFQT SVTAP
Sbjct: 1   MGVKGFVEGGIASIVAGCSTHPLDLIKVRMQLQGETPAPTGAVQNLRPALAFQTNSVTAP 60

Query: 61  RSMNIPPPPPPAARVGPVAVGIRIVQQEGVAALFSGVSATVLRQTMYSTTRMGLYDILKQ 120
           RSMNIP PPPPAARVGPVAVG+RIVQ+EGVAALFSGVSATVLRQT+YSTTRMGLYD+LKQ
Sbjct: 61  RSMNIPSPPPPAARVGPVAVGVRIVQKEGVAALFSGVSATVLRQTLYSTTRMGLYDMLKQ 120

Query: 121 KWTDKDTGKMPLVRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPPAQRRNYKSVVDA 180
           KWTD+DTGK+PLVRKISAGLIAGA+GAAVGNPADVAMVRMQADGRLPPAQRRNYKSVVDA
Sbjct: 121 KWTDQDTGKLPLVRKISAGLIAGAIGAAVGNPADVAMVRMQADGRLPPAQRRNYKSVVDA 180

Query: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240
           ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF
Sbjct: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240

Query: 241 AAGFVAAVASNPVDVIKTRVMNMKVEPGAAAPYSGALDCALKTVRAEGPMALYKGFIPTI 300
           AAGFVAAVASNPVDVIKTRVMNMKVE GAAAPYSGALDCA+KTVRAEGPMALYKGFIPTI
Sbjct: 241 AAGFVAAVASNPVDVIKTRVMNMKVEEGAAAPYSGALDCAMKTVRAEGPMALYKGFIPTI 300

Query: 301 SRQGPFTVVLFVTLEQVRKVFKDF 325
           SRQGPFTVVLFVTLEQVRKV KDF
Sbjct: 301 SRQGPFTVVLFVTLEQVRKVLKDF 324

BLAST of Spg013372 vs. ExPASy TrEMBL
Match: A0A6J1GDC4 (mitochondrial uncoupling protein 5-like OS=Cucurbita moschata OX=3662 GN=LOC111452954 PE=3 SV=1)

HSP 1 Score: 591.7 bits (1524), Expect = 1.9e-165
Identity = 306/324 (94.44%), Postives = 314/324 (96.91%), Query Frame = 0

Query: 1   MGFKGFLEGGIASIVAGCSTHPLDLIKVRMQLQGETQAPAAAVHNLRPALAFQTTSVTAP 60
           MG KGF+EGGIASIVAGCSTHPLDLIKVRMQLQGE  AP  AV NLRPALAFQTTSVTAP
Sbjct: 1   MGVKGFVEGGIASIVAGCSTHPLDLIKVRMQLQGEAPAPTGAVQNLRPALAFQTTSVTAP 60

Query: 61  RSMNIPPPPPPAARVGPVAVGIRIVQQEGVAALFSGVSATVLRQTMYSTTRMGLYDILKQ 120
           RSMNIP PPPPAARVGPVAVG+RIVQQEGVAALFSGVSATVLRQT+YSTTRMGLYD+LKQ
Sbjct: 61  RSMNIPSPPPPAARVGPVAVGVRIVQQEGVAALFSGVSATVLRQTLYSTTRMGLYDMLKQ 120

Query: 121 KWTDKDTGKMPLVRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPPAQRRNYKSVVDA 180
           KWTD+DTGK+PLVRKISAGLIAGA+GAAVGNPADVAMVRMQADGRLPPAQRRNYKSVVDA
Sbjct: 121 KWTDQDTGKLPLVRKISAGLIAGAIGAAVGNPADVAMVRMQADGRLPPAQRRNYKSVVDA 180

Query: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240
           ITQM RGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF
Sbjct: 181 ITQMVRGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240

Query: 241 AAGFVAAVASNPVDVIKTRVMNMKVEPGAAAPYSGALDCALKTVRAEGPMALYKGFIPTI 300
           AAGFVAAVASNPVDVIKTRVMNMKVE GAAAPYSGALDCA+KTVRAEGPMALYKGFIPTI
Sbjct: 241 AAGFVAAVASNPVDVIKTRVMNMKVEAGAAAPYSGALDCAMKTVRAEGPMALYKGFIPTI 300

Query: 301 SRQGPFTVVLFVTLEQVRKVFKDF 325
           SRQGPFTVVLFVTLEQVRKV KDF
Sbjct: 301 SRQGPFTVVLFVTLEQVRKVLKDF 324

BLAST of Spg013372 vs. TAIR 10
Match: AT2G22500.1 (uncoupling protein 5 )

HSP 1 Score: 474.9 bits (1221), Expect = 5.0e-134
Identity = 248/324 (76.54%), Postives = 281/324 (86.73%), Query Frame = 0

Query: 1   MGFKGFLEGGIASIVAGCSTHPLDLIKVRMQLQGETQAPAAAVHNLRPALAFQTTSVTAP 60
           MG KGF EGGIASIVAGCSTHPLDLIKVRMQLQGE+   A    NLRPALAFQT++    
Sbjct: 1   MGLKGFAEGGIASIVAGCSTHPLDLIKVRMQLQGES---APIQTNLRPALAFQTST---- 60

Query: 61  RSMNIPPPPPPAARVGPVAVGIRIVQQEGVAALFSGVSATVLRQTMYSTTRMGLYDILKQ 120
            ++N PP      RVG + VG R++++EG+ ALFSGVSATVLRQT+YSTTRMGLYDI+K 
Sbjct: 61  -TVNAPP-----LRVGVIGVGSRLIREEGMRALFSGVSATVLRQTLYSTTRMGLYDIIKG 120

Query: 121 KWTDKDTGKMPLVRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPPAQRRNYKSVVDA 180
           +WTD +T  MPL++KI AG IAGA+GAAVGNPADVAMVRMQADGRLP   RRNYKSV+DA
Sbjct: 121 EWTDPETKTMPLMKKIGAGAIAGAIGAAVGNPADVAMVRMQADGRLPLTDRRNYKSVLDA 180

Query: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240
           ITQM RGEGVTSLWRGSSLT+NRAMLVT+SQLASYD +KETIL+KGL+KDGLGTHV+ASF
Sbjct: 181 ITQMIRGEGVTSLWRGSSLTINRAMLVTSSQLASYDSVKETILEKGLLKDGLGTHVSASF 240

Query: 241 AAGFVAAVASNPVDVIKTRVMNMKVEPGAAAPYSGALDCALKTVRAEGPMALYKGFIPTI 300
           AAGFVA+VASNPVDVIKTRVMNMKV  G A PY GA+DCALKTV+AEG M+LYKGFIPT+
Sbjct: 241 AAGFVASVASNPVDVIKTRVMNMKVVAGVAPPYKGAVDCALKTVKAEGIMSLYKGFIPTV 300

Query: 301 SRQGPFTVVLFVTLEQVRKVFKDF 325
           SRQ PFTVVLFVTLEQV+K+FKD+
Sbjct: 301 SRQAPFTVVLFVTLEQVKKLFKDY 311

BLAST of Spg013372 vs. TAIR 10
Match: AT4G24570.1 (dicarboxylate carrier 2 )

HSP 1 Score: 435.6 bits (1119), Expect = 3.4e-122
Identity = 229/324 (70.68%), Postives = 263/324 (81.17%), Query Frame = 0

Query: 1   MGFKGFLEGGIASIVAGCSTHPLDLIKVRMQLQGETQAPAAAVHNLRPALAFQTTSVTAP 60
           MG K F+EGGIAS++AGCSTHPLDLIKVR+QL GE  +    V  LRPALAF  +S  A 
Sbjct: 1   MGVKSFVEGGIASVIAGCSTHPLDLIKVRLQLHGEAPS-TTTVTLLRPALAFPNSSPAAF 60

Query: 61  RSMNIPPPPPPAARVGPVAVGIRIVQQEGVAALFSGVSATVLRQTMYSTTRMGLYDILKQ 120
                  P     +VGP+++GI IV+ EG AALFSGVSAT+LRQT+YSTTRMGLY++LK 
Sbjct: 61  LETTSSVP-----KVGPISLGINIVKSEGAAALFSGVSATLLRQTLYSTTRMGLYEVLKN 120

Query: 121 KWTDKDTGKMPLVRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPPAQRRNYKSVVDA 180
           KWTD ++GK+ L RKI AGL+AG +GAAVGNPADVAMVRMQADGRLP AQRRNY  V DA
Sbjct: 121 KWTDPESGKLNLSRKIGAGLVAGGIGAAVGNPADVAMVRMQADGRLPLAQRRNYAGVGDA 180

Query: 181 ITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASF 240
           I  M +GEGVTSLWRGS+LT+NRAM+VTA+QLASYDQ KE IL+ G+M DGLGTHV ASF
Sbjct: 181 IRSMVKGEGVTSLWRGSALTINRAMIVTAAQLASYDQFKEGILENGVMNDGLGTHVVASF 240

Query: 241 AAGFVAAVASNPVDVIKTRVMNMKVEPGAAAPYSGALDCALKTVRAEGPMALYKGFIPTI 300
           AAGFVA+VASNPVDVIKTRVMNMKV       Y GA DCA+KTV+AEG MALYKGF+PT+
Sbjct: 241 AAGFVASVASNPVDVIKTRVMNMKV-----GAYDGAWDCAVKTVKAEGAMALYKGFVPTV 300

Query: 301 SRQGPFTVVLFVTLEQVRKVFKDF 325
            RQGPFTVVLFVTLEQVRK+ +DF
Sbjct: 301 CRQGPFTVVLFVTLEQVRKLLRDF 313

BLAST of Spg013372 vs. TAIR 10
Match: AT5G09470.1 (dicarboxylate carrier 3 )

HSP 1 Score: 369.0 bits (946), Expect = 3.9e-102
Identity = 211/339 (62.24%), Postives = 248/339 (73.16%), Query Frame = 0

Query: 1   MGFKGFLEGGIASIVAGCSTHPLDLIKVRMQLQGE-----TQAP---AAAVHNL-----R 60
           MGFK FLEGGIA+I+AG  THPLDLIKVRMQLQGE      Q P    +  HNL     R
Sbjct: 1   MGFKPFLEGGIAAIIAGALTHPLDLIKVRMQLQGEHSFSLDQNPNPNLSLDHNLPVKPYR 60

Query: 61  PALAFQTT--SVTAPRSMNIPPPPPPAARVGPVAVGIRIVQQEGVAALFSGVSATVLRQT 120
           P  A  +   S++        P     + + P AVG  IV+ EG AALFSGVSAT+LRQ 
Sbjct: 61  PVFALDSLIGSISLLPLHIHAPSSSTRSVMTPFAVGAHIVKTEGPAALFSGVSATILRQM 120

Query: 121 MYSTTRMGLYDILKQKWTDKDTGKMPLVRKISAGLIAGAVGAAVGNPADVAMVRMQADGR 180
           +YS TRMG+YD LK++WTD+ TG  PLV KI+AGLIAGAVG+ VGNPADVAMVRMQADG 
Sbjct: 121 LYSATRMGIYDFLKRRWTDQLTGNFPLVTKITAGLIAGAVGSVVGNPADVAMVRMQADGS 180

Query: 181 LPPAQRRNYKSVVDAITQMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQK 240
           LP  +RRNYKSVVDAI ++AR EGV+SLWRGS LTVNRAM+VTASQLA+YD +KE ++  
Sbjct: 181 LPLNRRRNYKSVVDAIDRIARQEGVSSLWRGSWLTVNRAMIVTASQLATYDHVKEILVAG 240

Query: 241 GL-MKDGLGTHVTASFAAGFVAAVASNPVDVIKTRVMNMKVEPGAAAPYSGALDCALKTV 300
           G     G+GTHV ASFAAG VAAVASNP+DV+KTR+MN   E      Y G LDCA+K V
Sbjct: 241 GRGTPGGIGTHVAASFAAGIVAAVASNPIDVVKTRMMNADKE-----IYGGPLDCAVKMV 300

Query: 301 RAEGPMALYKGFIPTISRQGPFTVVLFVTLEQVRKVFKD 324
             EGPMALYKG +PT +RQGPFT++LF+TLEQVR + KD
Sbjct: 301 AEEGPMALYKGLVPTATRQGPFTMILFLTLEQVRGLLKD 334

BLAST of Spg013372 vs. TAIR 10
Match: AT3G54110.1 (plant uncoupling mitochondrial protein 1 )

HSP 1 Score: 199.5 bits (506), Expect = 4.1e-51
Identity = 121/321 (37.69%), Postives = 179/321 (55.76%), Query Frame = 0

Query: 4   KGFLEGGIASIVAGCSTHPLDLIKVRMQLQGETQAPAAAVHNLRPALAFQTTSVTAPRSM 63
           K F     A+ V    T PLD  KVR+QLQ             + ALA     VT P+  
Sbjct: 13  KTFACSAFAACVGEVCTIPLDTAKVRLQLQ-------------KSALA---GDVTLPKYR 72

Query: 64  NIPPPPPPAARVGPVAVGIRIVQQEGVAALFSGVSATVLRQTMYSTTRMGLYDILKQKWT 123
            +         +G V     I ++EG+ +L+ GV   + RQ ++   R+G+Y+ +K  + 
Sbjct: 73  GL---------LGTVGT---IAREEGLRSLWKGVVPGLHRQCLFGGLRIGMYEPVKNLYV 132

Query: 124 DKD-TGKMPLVRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPPAQRRNYKSVVDAIT 183
            KD  G +PL +KI AGL  GA+G  V NP D+  VR+QA+G+L     R Y   ++A +
Sbjct: 133 GKDFVGDVPLSKKILAGLTTGALGIMVANPTDLVKVRLQAEGKLAAGAPRRYSGALNAYS 192

Query: 184 QMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLGTHVTASFAA 243
            + R EGV +LW G    V R  ++ A++LASYDQ+KETIL+     D + TH+ +   A
Sbjct: 193 TIVRQEGVRALWTGLGPNVARNAIINAAELASYDQVKETILKIPGFTDNVVTHILSGLGA 252

Query: 244 GFVAAVASNPVDVIKTRVMNMKVEPGAAAPYSGALDCALKTVRAEGPMALYKGFIPTISR 303
           GF A    +PVDV+K+R+M      G +  Y G +DC +KT++++GPMA YKGFIP   R
Sbjct: 253 GFFAVCIGSPVDVVKSRMM------GDSGAYKGTIDCFVKTLKSDGPMAFYKGFIPNFGR 299

Query: 304 QGPFTVVLFVTLEQVRKVFKD 324
            G + V++F+TLEQ +K  ++
Sbjct: 313 LGSWNVIMFLTLEQAKKYVRE 299

BLAST of Spg013372 vs. TAIR 10
Match: AT5G19760.1 (Mitochondrial substrate carrier family protein )

HSP 1 Score: 199.1 bits (505), Expect = 5.3e-51
Identity = 124/325 (38.15%), Postives = 179/325 (55.08%), Query Frame = 0

Query: 4   KGFLEGGIASIVAGCSTHPLDLIKVRMQLQGETQAPAAAVHNLRPALAFQTTSVTAPRSM 63
           K F+ GG + ++A C   P+D+IKVR+QL    Q  AA++          TT+       
Sbjct: 16  KPFVNGGASGMLATCVIQPIDMIKVRIQL---GQGSAASI----------TTN------- 75

Query: 64  NIPPPPPPAARVGPVAVGIRIVQQEGVAALFSGVSATVLRQTMYSTTRMGLYDILKQKWT 123
                               +++ EGV A + G+SA +LRQ  Y+T R+G + +L  K  
Sbjct: 76  --------------------MLKNEGVGAFYKGLSAGLLRQATYTTARLGSFKLLTAKAI 135

Query: 124 DKDTGK-MPLVRKISAGLIAGAVGAAVGNPADVAMVRMQADGRLPPAQRRNYKSVVDAIT 183
           + + GK +PL +K   GL AGA+GA VG+PAD+A++RMQAD  LP AQRRNY +   A+T
Sbjct: 136 ESNDGKPLPLYQKALCGLTAGAIGACVGSPADLALIRMQADNTLPLAQRRNYTNAFHALT 195

Query: 184 QMARGEGVTSLWRGSSLTVNRAMLVTASQLASYDQIKETILQKGLMKDGLG-----THVT 243
           +++  EGV +LW+G   TV RAM +    LASYDQ  E       M+D LG     T V 
Sbjct: 196 RISADEGVLALWKGCGPTVVRAMALNMGMLASYDQSAE------YMRDNLGFGEMSTVVG 255

Query: 244 ASFAAGFVAAVASNPVDVIKTRVMNMKVEPGAAAPYSGALDCALKTVRAEGPMALYKGFI 303
           AS  +GF AA  S P D +KT++  M+ +     PY+G+LDCA+KT++  GP+  Y GF 
Sbjct: 256 ASAVSGFCAAACSLPFDFVKTQIQKMQPDAQGKYPYTGSLDCAMKTLKEGGPLKFYSGFP 294

Query: 304 PTISRQGPFTVVLFVTLEQVRKVFK 323
               R  P  ++ ++ L Q+ K  K
Sbjct: 316 VYCVRIAPHVMMTWIFLNQITKFQK 294

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008440559.11.6e-16695.37PREDICTED: mitochondrial uncoupling protein 5-like [Cucumis melo] >KAA0036336.1 ... [more]
KAG6604059.13.6e-16695.06Mitochondrial uncoupling protein 5, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_038883604.14.7e-16695.37mitochondrial uncoupling protein 5-like [Benincasa hispida][more]
XP_004143448.18.0e-16694.75mitochondrial uncoupling protein 5 [Cucumis sativus] >KGN48714.1 hypothetical pr... [more]
XP_023544374.11.4e-16594.75mitochondrial uncoupling protein 5-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9SJY57.1e-13376.54Mitochondrial uncoupling protein 5 OS=Arabidopsis thaliana OX=3702 GN=PUMP5 PE=2... [more]
Q9SB524.8e-12170.68Mitochondrial uncoupling protein 4 OS=Arabidopsis thaliana OX=3702 GN=PUMP4 PE=2... [more]
Q9FY685.5e-10162.24Mitochondrial uncoupling protein 6 OS=Arabidopsis thaliana OX=3702 GN=PUMP6 PE=2... [more]
Q54PY72.0e-6343.89Probable mitochondrial 2-oxoglutarate/malate carrier protein OS=Dictyostelium di... [more]
Q9CR621.4e-5641.01Mitochondrial 2-oxoglutarate/malate carrier protein OS=Mus musculus OX=10090 GN=... [more]
Match NameE-valueIdentityDescription
A0A5A7T2E37.8e-16795.37Mitochondrial uncoupling protein 5-like OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3B1F27.8e-16795.37mitochondrial uncoupling protein 5-like OS=Cucumis melo OX=3656 GN=LOC103484939 ... [more]
A0A0A0KIS83.9e-16694.75Mitochondrial dicarboxylate carrier protein OS=Cucumis sativus OX=3659 GN=Csa_6G... [more]
A0A6J1IN721.9e-16594.44mitochondrial uncoupling protein 5-like OS=Cucurbita maxima OX=3661 GN=LOC111477... [more]
A0A6J1GDC41.9e-16594.44mitochondrial uncoupling protein 5-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
AT2G22500.15.0e-13476.54uncoupling protein 5 [more]
AT4G24570.13.4e-12270.68dicarboxylate carrier 2 [more]
AT5G09470.13.9e-10262.24dicarboxylate carrier 3 [more]
AT3G54110.14.1e-5137.69plant uncoupling mitochondrial protein 1 [more]
AT5G19760.15.3e-5138.15Mitochondrial substrate carrier family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002030Mitochondrial carrier UCP-likePRINTSPR00784MTUNCOUPLINGcoord: 309..324
score: 23.78
coord: 227..245
score: 38.06
coord: 115..127
score: 33.73
coord: 184..201
score: 41.45
IPR023395Mitochondrial carrier domain superfamilyGENE3D1.50.40.10Mitochondrial carrier domaincoord: 4..323
e-value: 2.2E-84
score: 284.8
IPR023395Mitochondrial carrier domain superfamilySUPERFAMILY103506Mitochondrial carriercoord: 4..317
IPR018108Mitochondrial substrate/solute carrierPFAMPF00153Mito_carrcoord: 233..322
e-value: 6.4E-19
score: 67.7
coord: 4..124
e-value: 5.5E-17
score: 61.5
coord: 130..224
e-value: 3.0E-18
score: 65.5
IPR018108Mitochondrial substrate/solute carrierPROSITEPS50920SOLCARcoord: 130..221
score: 26.009867
IPR018108Mitochondrial substrate/solute carrierPROSITEPS50920SOLCARcoord: 230..320
score: 25.795336
IPR018108Mitochondrial substrate/solute carrierPROSITEPS50920SOLCARcoord: 4..121
score: 17.535891
NoneNo IPR availablePANTHERPTHR45618MITOCHONDRIAL DICARBOXYLATE CARRIER-RELATEDcoord: 1..324
NoneNo IPR availablePANTHERPTHR45618:SF45MITOCHONDRIAL UNCOUPLING PROTEIN 5coord: 1..324

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg013372.1Spg013372.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071423 malate transmembrane transport
biological_process GO:0006839 mitochondrial transport
biological_process GO:1902356 oxaloacetate(2-) transmembrane transport
biological_process GO:0035435 phosphate ion transmembrane transport
biological_process GO:0071422 succinate transmembrane transport
biological_process GO:1902358 sulfate transmembrane transport
biological_process GO:0015709 thiosulfate transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0031966 mitochondrial membrane
molecular_function GO:0015297 antiporter activity
molecular_function GO:0015140 malate transmembrane transporter activity
molecular_function GO:0015131 oxaloacetate transmembrane transporter activity
molecular_function GO:0015141 succinate transmembrane transporter activity
molecular_function GO:0015116 sulfate transmembrane transporter activity
molecular_function GO:0015117 thiosulfate transmembrane transporter activity