Spg013281 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg013281
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionProtein DETOXIFICATION
Locationscaffold1: 6967746 .. 6972141 (-)
RNA-Seq ExpressionSpg013281
SyntenySpg013281
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTGAACAAGAACAACATACAGAATTCCCCTTGTTGGAGGATTCGGCTGCAATAATATTACAACCTCATGATCATGGTGATGGAGATGAGGAGGATCAAAATTGTGAACATATTGTAACGAGGGTGTGGATCGAATCCAAGAAGTTATGGCATATTGTGGGCCCTGCAATCTTCAGCAGGGTAACCTCGTACGCCGTGCTCGTTCTGTCCCAAGCCTCAGCCGGCCATCTGGGCGACCTTGACCTTGCTGCCTTTTCCATCGCCATTAACGTCATCGTCGGCCTCGACTTGGGCCTTCTGATGGGGATGTCAAGCGCGTTGGAGACGCTGTGCGGGCAGGCGTACGGGGCCAAGAAGCACTACATGCTGGGAGTGTATCTGCAGCGCTCCTGGATCGTTCTTCTCGTTTGCTGCATTTTGCTGTTGCCCGTGTTCGTGTTTGCTTCTCCGATTCTGAGGGCGATAGGGGAGCCTGATGACTTGGCGGAGCTGGCTGGGGTTGTGGCCAGATGGTTGATTCCGCTGCACTTCAGCTTCGCGTTTTACTTGCCGTTGCAGAGATTCTTGCAGAGCCAAGCCAAGGCGGGGGCGATTATGTGGACTGGGGTGGTGGCGCTTGTGGCGTATGCAGTGGCGAGTTGGGTGTTTGTGGGTCGCCTGAAAATGGGAGTGGCTGGCATTGCGGTGGCTTGCAATGTTGCTTGGGCAACTATGCCCGTTATTCTGATGGGTTATAGTGTGTGCGGCGGATGTCCGCAGACTTGGATTGGTTTTTCCGTTAAAGCCTTCTCGGATCTCTGGGAGTTTGTCAAGCTCTCTGCTGCTTCAGGGCTTATGCTATGGTCTCTCCTCTCATTCAACTCACTTTTTTTTTTTCCTCCAAAACTAGGATCCATTTCAAATACATTTTAATTTAAATGTTTTTTAAGAACGCATTTTTTAAAAAACATTTTGAATAAAAATTGTTAAGAATTTAAACACTGATATGTTTGGTTACATTTCCTCGTAAATGTTTTTCTACACAAGTTATTGTTTATTTAAAAAGTCTTTTTTTAAAAGTTGTTTCAATTGTTTCTCTCGAAACGGTTTATTTATCAATCTCATTTTCTAATAAGTCATTTTTAGTGGTTGTCAAACACTATAATTTTTTTCAAAATGATGTATTTTCAAAATTAAACACTTGAAAAGTTAAATCAAACACACCCCTAGTATAGTTTCGCTATATATATATATATATATTAATTATACCCTTTTAGGTTGTCGGTCCTAAAAATAACCCTAACTATTTATTAGAAATATAGTTGAGAGGAATTAAGCTACTTAAAAAATAAATATCTAAGAAATTTGGAGGAGGAACTAATAATTTTTTGGTGTCTTAATTATTCTGTTCCACCAAGCTAATCAAGCTAATTAATTAGTTTTTTATTACCCTCAAATCTTGTTGAACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTATATATTTGATCTCGTTTTTCTGTGTTTTGCATTTTCTGTTTTATATTGGCTTATTATATTCAACCACATGCATATATTTTATGCATTTTTTTGTTTTTGACTAATTTGTTGCATATCGGTATGTATGACACCATTAAAATTTTGCCAGTTAGGAGAGAGAAGCTAATCAAGCTAATTAATTAGTTTTTTAATTGTCAAAGTTAGTATAATTGAATGGCAATTGACATGTATTATCGATCAAAAGGTCAGAGTTTTGAAATCCCCACCCTCAAATCTTATTGAACTTAAAATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTTGATCTAGTTTTTCTGTGTTTTACATTTTCTGTTTTATATTGGCTTATTATATTCAGCCACATGCATCTATTTTCTGCATTTTTTTTTGTTTTGAGTAATTTGTTGCATATCGGTATGTATGACACCATTAAAATGTTGCCAGTTGAGAGAGAGAAGCTAATCAAGCTAATTAATTAATTTTTTAATTGTCAAAATGAGTATAACTAAATGGTAATTGACATGTATTATCAATCAAAAAGTCAGAGTTTTGAAATTCCCACCCTCAAATCTTGTTGAACTAAAAAAAAAAAATAATAAAATTATATATTTGTGATCTCGTTTGTCTATGTTTTGAATTTTCTGTTTTATATTGGCTTATTATATTCAACCACATGCATATATTTTATGCATTTTTGTATGTATGACACCATTAAAATTTTGCCAGTTAAGAGAGAGAAGCTAATCAAGCTAATTAATTAGTTTTTTAATTGTCAAAATGAGTATAACTAAATGGTAATTGACATGTATTATCGATCAAAAAGTCAGAGTTTTGAAATTCCCACCCTCAAATCTTGTTGAACTCAAAAAAAAATATATATATATATATTTGATATCGTTTTTCTGTTTTATATTGGCTTATTATATTCAACGACCACTTGCATCTATTTAATGCTTTTTTTTTGGTTTTGACTAATTTGTTGCATATCGGTATGTATGGCACCATTAAAATGTTGCTAGTTAGGAGAGAGAAGCTAATCAAGCTAATTAATTAGTTTTTTAATTGAGTATAAGTGAATGGTAATTGACATGTATTATCGATCAAAAGGTCAGAGTTTTGAAATCCTCACCCTTAAATCTTGTTGAACTCAAAAAAAAAAAAAATAATAATAATTATTATATATTTGGTCTCGTCTTTCTGTGTTTTGCATTTTCTTTTTTATATTGGCTTATTATATTCAACCACATGCATCAATTTTCTGCATTTTTTTTTTGTTTTGATTAATTTGTTGCATATGGGTATGTATGGCACCATTAAAATGTTGCCAGGAGATAGAAAAAAAAAGTTTTTTCATTTTCTACCGTTCTCTTTCGTAACTGTGAAAATTTTAATGAGATGTGTAGGAAGATCCATACATACCGATATGCAGTCAAGTATTTTTTTTTTTTGAAAAATATATGTTTTATGTGATAATTAATATCACGTATATTTGTTCCATTGATTTTGAGATGGAAACCAAGGTTTATCTAATACATTCTTCTGGTGTCAATTTATTTAATCAAACTGACATCTCTATTCCCAAATATTAAAATTTCAGCTTGGAAACATGGTACTACAGAATACTGATAATGGTGACTGGAAACATGAAGAACGCTGAAGTTGCAGTGGATGCTGTATCTATCTGGTCCTCTACATATCCTTTCACTCTCTAAATTAATTTTCTTAGAGACCAAACATGTTTTACCGAAATGAATGAACTAAACACTTGGGTTTGTGGTCATTTTTTGATGGGCGCAGCATGAACATCAACGGTTGGGAAATGATGATTCCTCTAGGGTTCTTTGTGGGTACTGCGTAAGTTATAAACCATCATTATTCAATTTATAAAAAAAAAAAGTCTCCGAATTTATATATATAAATACGTGACATATTAAACAGAGTGAGAGTGGCGAATGAGCTCGGGGCAGGCAATGGAAAAGGAGCCAAGTTTGCTACCATTGTTTCATCGGCGACATCATTAATAATTGGCATTGTCATTTGTTGTTTGATTGTAATCTTTCACGACAAATTAGGTCTTTTTTACTCCTCCAGCAACCTTGTCCTTAAAGAAGTCGATGAGCTTAGTGTTCTCTTGGCTTTCAGCATTCTCCTCAACAGTGTCCAACCAGTTCTTTCTGGTATGTCATATTTAATTCCTTTATTTATATATATATTTTTTAAATTAGGTGCATTATTAAGATATCAAGTTGCAATATTTTCAGGGGTGGCAGTGGGGTCAGGTTGGCAATCTTACGTGGCTTATATAAACTTGGGATGCTATTATCTCATCGGATTGCCTCTAGGGATTTTGATTCAATCGTTTACCAACCTTGGAGTTAAGGTATATAATCTACATAATATGATATTTACATTTTAATTTCATGCATGCAGTTATATATTGAATAACAATATCATCTCTATATATGATTATATAATTTATTTTGTGCATCGATCATATATGCAATAGGGAATGTGGATGGGAATGATATTTGGAGGAACTGGAATTCAGACATTGATTTTACTCATCATTACCAGTCAATGTGATTGGGATGGAGAGGTGTGTAAAAAAAATTTATGTTTAAAATTCTCTAACATCACTTATTTTTCAATTTATATTATGAATAATTAATTAATGTTAGGATAGGATATAATTTCACGAAACAAATGAGCAGATAAAATAAAATGTTAGAATAAAAACTAAAATGTACCTTATCCTAGAGATTTAATAAACTTTTGGTTTTGTTTGGATGGTTGATTTGCAGGCTAGGAAAGCGAGGTCGCACGTGAAGAAATGGACAGATGAGAAATTTGAGCTAGAAGAATGA

mRNA sequence

ATGAGTGAACAAGAACAACATACAGAATTCCCCTTGTTGGAGGATTCGGCTGCAATAATATTACAACCTCATGATCATGGTGATGGAGATGAGGAGGATCAAAATTGTGAACATATTGTAACGAGGGTGTGGATCGAATCCAAGAAGTTATGGCATATTGTGGGCCCTGCAATCTTCAGCAGGGTAACCTCGTACGCCGTGCTCGTTCTGTCCCAAGCCTCAGCCGGCCATCTGGGCGACCTTGACCTTGCTGCCTTTTCCATCGCCATTAACGTCATCGTCGGCCTCGACTTGGGCCTTCTGATGGGGATGTCAAGCGCGTTGGAGACGCTGTGCGGGCAGGCGTACGGGGCCAAGAAGCACTACATGCTGGGAGTGTATCTGCAGCGCTCCTGGATCGTTCTTCTCGTTTGCTGCATTTTGCTGTTGCCCGTGTTCGTGTTTGCTTCTCCGATTCTGAGGGCGATAGGGGAGCCTGATGACTTGGCGGAGCTGGCTGGGGTTGTGGCCAGATGGTTGATTCCGCTGCACTTCAGCTTCGCGTTTTACTTGCCGTTGCAGAGATTCTTGCAGAGCCAAGCCAAGGCGGGGGCGATTATGTGGACTGGGGTGGTGGCGCTTGTGGCGTATGCAGTGGCGAGTTGGGTGTTTGTGGGTCGCCTGAAAATGGGAGTGGCTGGCATTGCGGTGGCTTGCAATGTTGCTTGGGCAACTATGCCCGTTATTCTGATGGGTTATAGTGTGTGCGGCGGATGTCCGCAGACTTGGATTGGTTTTTCCGTTAAAGCCTTCTCGGATCTCTGGGAGTTTGTCAAGCTCTCTGCTGCTTCAGGGCTTATGCTATGCTTGGAAACATGGTACTACAGAATACTGATAATGGTGACTGGAAACATGAAGAACGCTGAAGTTGCAGTGGATGCTGTATCTATCTGCATGAACATCAACGGTTGGGAAATGATGATTCCTCTAGGGTTCTTTGTGGGTCTTTTTTACTCCTCCAGCAACCTTGTCCTTAAAGAAGTCGATGAGCTTAGTGTTCTCTTGGCTTTCAGCATTCTCCTCAACAGTGTCCAACCAGTTCTTTCTGGGGTGGCAGTGGGGTCAGGTTGGCAATCTTACGTGGCTTATATAAACTTGGGATGCTATTATCTCATCGGATTGCCTCTAGGGATTTTGATTCAATCGTTTACCAACCTTGGAGTTAAGGGAATGTGGATGGGAATGATATTTGGAGGAACTGGAATTCAGACATTGATTTTACTCATCATTACCAGTCAATGTGATTGGGATGGAGAGGCTAGGAAAGCGAGGTCGCACGTGAAGAAATGGACAGATGAGAAATTTGAGCTAGAAGAATGA

Coding sequence (CDS)

ATGAGTGAACAAGAACAACATACAGAATTCCCCTTGTTGGAGGATTCGGCTGCAATAATATTACAACCTCATGATCATGGTGATGGAGATGAGGAGGATCAAAATTGTGAACATATTGTAACGAGGGTGTGGATCGAATCCAAGAAGTTATGGCATATTGTGGGCCCTGCAATCTTCAGCAGGGTAACCTCGTACGCCGTGCTCGTTCTGTCCCAAGCCTCAGCCGGCCATCTGGGCGACCTTGACCTTGCTGCCTTTTCCATCGCCATTAACGTCATCGTCGGCCTCGACTTGGGCCTTCTGATGGGGATGTCAAGCGCGTTGGAGACGCTGTGCGGGCAGGCGTACGGGGCCAAGAAGCACTACATGCTGGGAGTGTATCTGCAGCGCTCCTGGATCGTTCTTCTCGTTTGCTGCATTTTGCTGTTGCCCGTGTTCGTGTTTGCTTCTCCGATTCTGAGGGCGATAGGGGAGCCTGATGACTTGGCGGAGCTGGCTGGGGTTGTGGCCAGATGGTTGATTCCGCTGCACTTCAGCTTCGCGTTTTACTTGCCGTTGCAGAGATTCTTGCAGAGCCAAGCCAAGGCGGGGGCGATTATGTGGACTGGGGTGGTGGCGCTTGTGGCGTATGCAGTGGCGAGTTGGGTGTTTGTGGGTCGCCTGAAAATGGGAGTGGCTGGCATTGCGGTGGCTTGCAATGTTGCTTGGGCAACTATGCCCGTTATTCTGATGGGTTATAGTGTGTGCGGCGGATGTCCGCAGACTTGGATTGGTTTTTCCGTTAAAGCCTTCTCGGATCTCTGGGAGTTTGTCAAGCTCTCTGCTGCTTCAGGGCTTATGCTATGCTTGGAAACATGGTACTACAGAATACTGATAATGGTGACTGGAAACATGAAGAACGCTGAAGTTGCAGTGGATGCTGTATCTATCTGCATGAACATCAACGGTTGGGAAATGATGATTCCTCTAGGGTTCTTTGTGGGTCTTTTTTACTCCTCCAGCAACCTTGTCCTTAAAGAAGTCGATGAGCTTAGTGTTCTCTTGGCTTTCAGCATTCTCCTCAACAGTGTCCAACCAGTTCTTTCTGGGGTGGCAGTGGGGTCAGGTTGGCAATCTTACGTGGCTTATATAAACTTGGGATGCTATTATCTCATCGGATTGCCTCTAGGGATTTTGATTCAATCGTTTACCAACCTTGGAGTTAAGGGAATGTGGATGGGAATGATATTTGGAGGAACTGGAATTCAGACATTGATTTTACTCATCATTACCAGTCAATGTGATTGGGATGGAGAGGCTAGGAAAGCGAGGTCGCACGTGAAGAAATGGACAGATGAGAAATTTGAGCTAGAAGAATGA

Protein sequence

MSEQEQHTEFPLLEDSAAIILQPHDHGDGDEEDQNCEHIVTRVWIESKKLWHIVGPAIFSRVTSYAVLVLSQASAGHLGDLDLAAFSIAINVIVGLDLGLLMGMSSALETLCGQAYGAKKHYMLGVYLQRSWIVLLVCCILLLPVFVFASPILRAIGEPDDLAELAGVVARWLIPLHFSFAFYLPLQRFLQSQAKAGAIMWTGVVALVAYAVASWVFVGRLKMGVAGIAVACNVAWATMPVILMGYSVCGGCPQTWIGFSVKAFSDLWEFVKLSAASGLMLCLETWYYRILIMVTGNMKNAEVAVDAVSICMNINGWEMMIPLGFFVGLFYSSSNLVLKEVDELSVLLAFSILLNSVQPVLSGVAVGSGWQSYVAYINLGCYYLIGLPLGILIQSFTNLGVKGMWMGMIFGGTGIQTLILLIITSQCDWDGEARKARSHVKKWTDEKFELEE
Homology
BLAST of Spg013281 vs. NCBI nr
Match: XP_022950623.1 (protein DETOXIFICATION 27-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 638.3 bits (1645), Expect = 5.1e-179
Identity = 320/495 (64.65%), Postives = 384/495 (77.58%), Query Frame = 0

Query: 5   EQHTEFPLLEDSAAIILQPHDHGDGDEEDQNCEHIVTRVWIESKKLWHIVGPAIFSRVTS 64
           EQ    PLL+D +A  LQPH+           + IVTR WIESKKLWHIVGP+IFSR+ S
Sbjct: 3   EQQVNVPLLQDCSA-TLQPHN-----------QDIVTRFWIESKKLWHIVGPSIFSRIVS 62

Query: 65  YAVLVLSQASAGHLGDLDLAAFSIAINVIVGLDLGLLMGMSSALETLCGQAYGAKKHYML 124
           Y++L+++QA AGHL DLDLAAFSIA+ V++G D+GLLMGM+SALETLCGQAYGAKK+YML
Sbjct: 63  YSILIIAQAFAGHLNDLDLAAFSIAVTVVIGFDMGLLMGMASALETLCGQAYGAKKYYML 122

Query: 125 GVYLQRSWIVLLVCCILLLPVFVFASPILRAIGEPDDLAELAGVVARWLIPLHFSFAFYL 184
           G+Y+QRSWIVL +CCILLLP+F FASPIL+ IGEP DLA+LAGV++ W++P+HFSFAFY 
Sbjct: 123 GIYMQRSWIVLFLCCILLLPIFFFASPILKLIGEPHDLADLAGVLSIWMLPIHFSFAFYF 182

Query: 185 PLQRFLQSQAKAGAIMWTGVVALVAYAVASWVFVGRLKMGVAGIAVACNVAWATMPVILM 244
           PLQRFLQSQ K  AI+W+ V ALV++ VASWV V +LKMGV GIAVACNV W  MP++ +
Sbjct: 183 PLQRFLQSQVKVWAIVWSAVAALVSHLVASWVLVVQLKMGVVGIAVACNVGWCAMPIVQI 242

Query: 245 GYSVCGGCPQTWIGFSVKAFSDLWEFVKLSAASGLMLCLETWYYRILIMVTGNMKNAEVA 304
            Y+ CGGCP TW GFSV+AFS LWEFVKLSAASG+MLCLE WYYRIL+++TGNMKNAE+ 
Sbjct: 243 VYTTCGGCPLTWTGFSVEAFSGLWEFVKLSAASGVMLCLENWYYRILVVITGNMKNAEIM 302

Query: 305 VDAVSICMNINGWEMMIPLGFFV------------------------------------- 364
           VDA+SICM+INGWEMMIPLGFFV                                     
Sbjct: 303 VDALSICMSINGWEMMIPLGFFVGTGVRVANELGAGNGEGAKFATIVSSATSLIIGLVFC 362

Query: 365 ----------GLFYSSSNLVLKEVDELSVLLAFSILLNSVQPVLSGVAVGSGWQSYVAYI 424
                     GL +S S +VL+EVD+L++LLA +IL NS+QPVLSGVAVGSGWQSYVAYI
Sbjct: 363 CLIVIFHNSFGLIFSPSAVVLQEVDKLTILLALTILFNSIQPVLSGVAVGSGWQSYVAYI 422

Query: 425 NLGCYYLIGLPLGILIQSFTNLGVKGMWMGMIFGGTGIQTLILLIITSQCDWDGEARKAR 453
           NLGCYY+IGLPLGI +Q FT+LGVKG+W+GMIFGGTGIQTLILL+IT +CDW+GEA+KA 
Sbjct: 423 NLGCYYIIGLPLGIFMQWFTDLGVKGIWIGMIFGGTGIQTLILLLITIRCDWEGEAKKAG 482

BLAST of Spg013281 vs. NCBI nr
Match: XP_023544211.1 (protein DETOXIFICATION 27-like isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 636.0 bits (1639), Expect = 2.6e-178
Identity = 319/495 (64.44%), Postives = 383/495 (77.37%), Query Frame = 0

Query: 5   EQHTEFPLLEDSAAIILQPHDHGDGDEEDQNCEHIVTRVWIESKKLWHIVGPAIFSRVTS 64
           EQ    PLL+D +A  LQPH+           + IV R WIESKKLWHIVGP+IFSR+ S
Sbjct: 3   EQQVNVPLLQDCSA-TLQPHN-----------QDIVARFWIESKKLWHIVGPSIFSRIIS 62

Query: 65  YAVLVLSQASAGHLGDLDLAAFSIAINVIVGLDLGLLMGMSSALETLCGQAYGAKKHYML 124
           Y++L+++QA AGHL DLDLAAFSIA+ V++G D+GLLMGM+SALETLCGQAYGAKK+YML
Sbjct: 63  YSILIIAQAFAGHLNDLDLAAFSIAVTVVIGFDMGLLMGMASALETLCGQAYGAKKYYML 122

Query: 125 GVYLQRSWIVLLVCCILLLPVFVFASPILRAIGEPDDLAELAGVVARWLIPLHFSFAFYL 184
           G+Y+QRSWIVL +CC+LLLP+F FASPIL+ IGEP DLA+LAGV++ W++P+HFSFAFY 
Sbjct: 123 GIYMQRSWIVLFLCCVLLLPIFFFASPILKLIGEPHDLADLAGVLSIWMLPIHFSFAFYF 182

Query: 185 PLQRFLQSQAKAGAIMWTGVVALVAYAVASWVFVGRLKMGVAGIAVACNVAWATMPVILM 244
           PLQRFLQSQ K  AI+W+ V ALV++ VASWV V +LKMGV GIAVACNV W  MP++ +
Sbjct: 183 PLQRFLQSQVKVWAIVWSAVAALVSHLVASWVLVVQLKMGVVGIAVACNVGWCAMPIVQI 242

Query: 245 GYSVCGGCPQTWIGFSVKAFSDLWEFVKLSAASGLMLCLETWYYRILIMVTGNMKNAEVA 304
            Y+ CGGCP TW GFSV+AFS LWEFVKLSAASG+MLCLE WYYRIL+++TGNMKNAE+ 
Sbjct: 243 VYTTCGGCPLTWTGFSVEAFSGLWEFVKLSAASGVMLCLENWYYRILVVITGNMKNAEIM 302

Query: 305 VDAVSICMNINGWEMMIPLGFFV------------------------------------- 364
           VDA+SICM+INGWEMMIPLGFFV                                     
Sbjct: 303 VDALSICMSINGWEMMIPLGFFVGTGVRVANELGAGNGEGAKFATIVSSATSLLLGLVFC 362

Query: 365 ----------GLFYSSSNLVLKEVDELSVLLAFSILLNSVQPVLSGVAVGSGWQSYVAYI 424
                     GL +SSS +VL+EVD+L++LLA +IL NS+QPVLSGVAVGSGWQSYVAYI
Sbjct: 363 CLIVIFHNSFGLIFSSSAVVLQEVDKLTILLALTILFNSIQPVLSGVAVGSGWQSYVAYI 422

Query: 425 NLGCYYLIGLPLGILIQSFTNLGVKGMWMGMIFGGTGIQTLILLIITSQCDWDGEARKAR 453
           NLGCYY+IGLPLGI +Q FT+LGVKG+W+GMIFGGTGIQTLILLIIT +CDW+GEA+KA 
Sbjct: 423 NLGCYYIIGLPLGIFMQWFTDLGVKGIWIGMIFGGTGIQTLILLIITIRCDWEGEAKKAG 482

BLAST of Spg013281 vs. NCBI nr
Match: XP_022978468.1 (protein DETOXIFICATION 27-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 635.6 bits (1638), Expect = 3.3e-178
Identity = 317/495 (64.04%), Postives = 384/495 (77.58%), Query Frame = 0

Query: 5   EQHTEFPLLEDSAAIILQPHDHGDGDEEDQNCEHIVTRVWIESKKLWHIVGPAIFSRVTS 64
           EQ    PLL+D +A  LQPH+           + IVTR WIESKKLW+IVGP+IFSR+ S
Sbjct: 3   EQQVNVPLLQDCSA-TLQPHN-----------QDIVTRFWIESKKLWNIVGPSIFSRIIS 62

Query: 65  YAVLVLSQASAGHLGDLDLAAFSIAINVIVGLDLGLLMGMSSALETLCGQAYGAKKHYML 124
           Y++L+++QA AGHL DLDLAAFSIA+ V++G D+GLLMGM+SALETLCGQAYGAKK+YML
Sbjct: 63  YSILIIAQAFAGHLNDLDLAAFSIAVTVVIGFDMGLLMGMASALETLCGQAYGAKKYYML 122

Query: 125 GVYLQRSWIVLLVCCILLLPVFVFASPILRAIGEPDDLAELAGVVARWLIPLHFSFAFYL 184
           G+Y+QRSWIVL++CC+LLLP+F FASPIL+ IGEP DLA+LAGV++ W++P+HFSFAFY 
Sbjct: 123 GIYMQRSWIVLILCCVLLLPIFFFASPILKLIGEPHDLADLAGVLSIWMLPIHFSFAFYF 182

Query: 185 PLQRFLQSQAKAGAIMWTGVVALVAYAVASWVFVGRLKMGVAGIAVACNVAWATMPVILM 244
           PLQRFLQSQ K  AI+W+ V ALV++ VASWV V +LKMGV GIAVACN+ W  MP + +
Sbjct: 183 PLQRFLQSQVKVWAIVWSAVAALVSHLVASWVLVVQLKMGVVGIAVACNIGWCAMPTVQI 242

Query: 245 GYSVCGGCPQTWIGFSVKAFSDLWEFVKLSAASGLMLCLETWYYRILIMVTGNMKNAEVA 304
            Y+ CGGCP TW GFSV+AFS LWEFVKLSAASG+MLCLE WYYRIL+++TGNMKNAE+ 
Sbjct: 243 VYTTCGGCPLTWTGFSVEAFSGLWEFVKLSAASGVMLCLENWYYRILVVITGNMKNAEIM 302

Query: 305 VDAVSICMNINGWEMMIPLGFFV------------------------------------- 364
           VDA+SICM+INGWEMMIPLGFFV                                     
Sbjct: 303 VDALSICMSINGWEMMIPLGFFVGTGVRVANELGAGNGEGAKFATIVSSATSLIIGLVFC 362

Query: 365 ----------GLFYSSSNLVLKEVDELSVLLAFSILLNSVQPVLSGVAVGSGWQSYVAYI 424
                     GL +SSS +VL+EVD L++LLA +IL NS+QPVLSGVAVGSGWQSYVAYI
Sbjct: 363 CLIVIFHNSFGLIFSSSAVVLQEVDNLTILLALTILFNSIQPVLSGVAVGSGWQSYVAYI 422

Query: 425 NLGCYYLIGLPLGILIQSFTNLGVKGMWMGMIFGGTGIQTLILLIITSQCDWDGEARKAR 453
           NLGCYY+IGLPLGI +Q FT+LGVKG+W+GMIFGGTGIQTLILL++T +CDW+GEA+KA 
Sbjct: 423 NLGCYYIIGLPLGIFMQWFTDLGVKGIWIGMIFGGTGIQTLILLVVTIRCDWEGEAKKAG 482

BLAST of Spg013281 vs. NCBI nr
Match: KAG6604053.1 (Protein DETOXIFICATION 27, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 632.9 bits (1631), Expect = 2.2e-177
Identity = 318/492 (64.63%), Postives = 378/492 (76.83%), Query Frame = 0

Query: 5   EQHTEFPLLEDSAAIILQPHDHGDGDEEDQNCEHIVTRVWIESKKLWHIVGPAIFSRVTS 64
           +  T  PLLEDS  I+     H    ++DQN E IVTRVWIESKKLW IVGPA+ SR+++
Sbjct: 4   DHQTNVPLLEDSTEIL-----HPGDPDKDQNSEDIVTRVWIESKKLWQIVGPAVLSRIST 63

Query: 65  YAVLVLSQASAGHLGDLDLAAFSIAINVIVGLDLGLLMGMSSALETLCGQAYGAKKHYML 124
           Y+VLV+SQ  AGHLGDLDLAA SIA+NVI+G D GL+MGM+SALETLCGQAYGAKKHYML
Sbjct: 64  YSVLVISQVFAGHLGDLDLAAVSIALNVIIGFDFGLMMGMASALETLCGQAYGAKKHYML 123

Query: 125 GVYLQRSWIVLLVCCILLLPVFVFASPILRAIGEPDDLAELAGVVARWLIPLHFSFAFYL 184
           GVYLQRSW+VL +CC+LLLP  VFAS IL+ IGEPDDLA+LAGV+ARWLIPLHFS AFY 
Sbjct: 124 GVYLQRSWMVLFICCVLLLPALVFASSILKVIGEPDDLADLAGVLARWLIPLHFSLAFYF 183

Query: 185 PLQRFLQSQAKAGAIMWTGVVALVAYAVASWVFVGRLKMGVAGIAVACNVAWATMPVILM 244
           PLQRFLQSQ KAGAIMW  +V L  +  ASWVFVG LKMGV GIAVACN++W  +P+ LM
Sbjct: 184 PLQRFLQSQVKAGAIMWVAMVGLAVHVAASWVFVGFLKMGVVGIAVACNISWWVLPIGLM 243

Query: 245 GYSVCGGCPQTWIGFSVKAFSDLWEFVKLSAASGLMLCLETWYYRILIMVTGNMKNAEVA 304
            Y+ CGGCP TW GFSV+A + LW+F+KLSAASG+MLCLE WYY+ILI++TGNMKNA++ 
Sbjct: 244 AYTTCGGCPNTWTGFSVEALTGLWDFLKLSAASGVMLCLENWYYKILIVMTGNMKNAKIE 303

Query: 305 VDAVSICMNINGWEMMIPLGFFV------------------------------------- 364
           VDA+SICM+ING E+MIPL FF                                      
Sbjct: 304 VDALSICMSINGLEIMIPLAFFAGTGVRVANELGAGNGKGAKFAAIVSSATSLIIGIVFC 363

Query: 365 ----------GLFYSSSNLVLKEVDELSVLLAFSILLNSVQPVLSGVAVGSGWQSYVAYI 424
                     GL +SSS +VL+EV++LS+LLAF+IL NSVQPVLSGVAVGSGWQSYVAYI
Sbjct: 364 CLIVLLRDKFGLLFSSSTIVLQEVNKLSILLAFTILFNSVQPVLSGVAVGSGWQSYVAYI 423

Query: 425 NLGCYYLIGLPLGILIQSFTNLGVKGMWMGMIFGGTGIQTLILLIITSQCDWDGEARKAR 450
           NLGCYY IGLP+G+    F++LGVKG+WMGMIFGGTGIQTLILLIIT +CDW+GEA+KA 
Sbjct: 424 NLGCYYFIGLPVGMSTLKFSDLGVKGIWMGMIFGGTGIQTLILLIITIRCDWEGEAKKAS 483

BLAST of Spg013281 vs. NCBI nr
Match: XP_008441115.1 (PREDICTED: protein DETOXIFICATION 27-like [Cucumis melo])

HSP 1 Score: 632.9 bits (1631), Expect = 2.2e-177
Identity = 325/491 (66.19%), Postives = 382/491 (77.80%), Query Frame = 0

Query: 5   EQHTEFPLLEDSAAIILQPHDHGDGDEEDQNCEHIVTRVWIESKKLWHIVGPAIFSRVTS 64
           +Q    PLLE+S A ILQ HD     ++DQN E +V RVWIESKKLW+IVGPAI SRV++
Sbjct: 4   DQQISVPLLEESTA-ILQSHD----GDDDQNSEDLVRRVWIESKKLWYIVGPAILSRVST 63

Query: 65  YAVLVLSQASAGHLGDLDLAAFSIAINVIVGLDLGLLMGMSSALETLCGQAYGAKKHYML 124
           ++V+V SQA AGHLGDLDLAA SIA+NVI+G DLGL+MGM+SALETLCGQAYGAKKHYML
Sbjct: 64  HSVMVTSQAFAGHLGDLDLAAISIALNVIIGFDLGLMMGMASALETLCGQAYGAKKHYML 123

Query: 125 GVYLQRSWIVLLVCCILLLPVFVFASPILRAIGEPDDLAELAGVVARWLIPLHFSFAFYL 184
           GVYLQRSW+VL +CC+LLLPVF+FASPIL+AIGE D+LAELAG +ARWLIPLHFSFAFY 
Sbjct: 124 GVYLQRSWMVLFMCCVLLLPVFIFASPILKAIGEGDELAELAGGLARWLIPLHFSFAFYF 183

Query: 185 PLQRFLQSQAKAGAIMWTGVVALVAYAVASWVFVGRLKMGVAGIAVACNVAWATMPVILM 244
           PLQRFLQSQ KA AIMW  VV LV +  ASWVFVG LKMGV GIAVAC+++W  +P+ LM
Sbjct: 184 PLQRFLQSQVKARAIMWVAVVGLVVHVAASWVFVGLLKMGVVGIAVACDISWWVLPIGLM 243

Query: 245 GYSVCGGCPQTWIGFSVKAFSDLWEFVKLSAASGLMLCLETWYYRILIMVTGNMKNAEVA 304
           GYS  GGCP TW GFS++A S LW+F+KLSAASG+MLCLE WYY+ILI++TGNMKNA++ 
Sbjct: 244 GYSAGGGCPNTWTGFSLEALSGLWDFLKLSAASGVMLCLENWYYKILIVMTGNMKNAKIE 303

Query: 305 VDAVSICMNINGWEMMIPLGFFV------------------------------------- 364
           VDA+SICM ING E MIPL FF                                      
Sbjct: 304 VDALSICMGINGLEFMIPLAFFAGTGVRVANELGGGNGKGAKFAAIVSSTTSLVIGLLFC 363

Query: 365 ----------GLFYSSSNLVLKEVDELSVLLAFSILLNSVQPVLSGVAVGSGWQSYVAYI 424
                     GL +SS+++VL+EV++LS+LLAF+IL NSVQPVLSGVAVGSGWQSYVAYI
Sbjct: 364 CLIVIFHDKFGLLFSSTDIVLQEVNKLSILLAFTILFNSVQPVLSGVAVGSGWQSYVAYI 423

Query: 425 NLGCYYLIGLPLGILIQSFTNLGVKGMWMGMIFGGTGIQTLILLIITSQCDWDGEARKAR 449
           NLGCYY IGLPLGI    FT+LGVKG+W+GMIFGGTGIQTLILLIIT +CDW+ EA+KA 
Sbjct: 424 NLGCYYFIGLPLGIFTLRFTDLGVKGIWLGMIFGGTGIQTLILLIITIRCDWEEEAKKAS 483

BLAST of Spg013281 vs. ExPASy Swiss-Prot
Match: Q9FKQ1 (Protein DETOXIFICATION 27 OS=Arabidopsis thaliana OX=3702 GN=DTX27 PE=2 SV=1)

HSP 1 Score: 511.5 bits (1316), Expect = 9.5e-144
Identity = 262/479 (54.70%), Postives = 337/479 (70.35%), Query Frame = 0

Query: 14  EDSAAIILQPHDHGDGDEEDQNCEHIVTRVWIESKKLWHIVGPAIFSRVTSYAVLVLSQA 73
           E   A++  PH        +++ E +  R+ +E+KKLW IVGPAIFSRVT+Y++LV++QA
Sbjct: 11  ESRVALLKSPH------TAEEDGEGLKDRILVETKKLWQIVGPAIFSRVTTYSMLVITQA 70

Query: 74  SAGHLGDLDLAAFSIAINVIVGLDLGLLMGMSSALETLCGQAYGAKKHYMLGVYLQRSWI 133
            AGHLGDL+LAA SI  NV VG + GLL+GM+SALETLCGQA+GAKK++MLGVY+QRSWI
Sbjct: 71  FAGHLGDLELAAISIVNNVTVGFNFGLLLGMASALETLCGQAFGAKKYHMLGVYMQRSWI 130

Query: 134 VLLVCCILLLPVFVFASPILRAIGEPDDLAELAGVVARWLIPLHFSFAFYLPLQRFLQSQ 193
           VL  CC+LLLP ++F +P+L+ +G+PDD+AEL+GVVA W+IPLHF+F    PLQRFLQ Q
Sbjct: 131 VLFFCCVLLLPTYIFTTPVLKFLGQPDDIAELSGVVAIWVIPLHFAFTLSFPLQRFLQCQ 190

Query: 194 AKAGAIMWTGVVALVAYAVASWVFVGRLKMGVAGIAVACNVAWATMPVILMGYSVCGGCP 253
            K     +   VALV + +  W+FV  LK+GV G     +++W    +IL+ YS CGGCP
Sbjct: 191 LKNRVTAYAAAVALVVHILVCWLFVDGLKLGVVGTVATISISWWVNVLILLVYSTCGGCP 250

Query: 254 QTWIGFSVKAFSDLWEFVKLSAASGLMLCLETWYYRILIMVTGNMKNAEVAVDAVSICMN 313
            TW G S +A + LWEF+KLSA+SG+MLCLE WYYRILI++TGN++NA +AVD++SICM 
Sbjct: 251 LTWTGLSSEALTGLWEFLKLSASSGVMLCLENWYYRILIIMTGNLQNARIAVDSLSICMA 310

Query: 314 INGWEMMIPLGFF--------------------------------VGLF----------- 373
           INGWEMMIPL FF                                +GLF           
Sbjct: 311 INGWEMMIPLAFFAGTGVRVANELGAGNGKGARFATIVSVTQSLIIGLFFWVLIMLLHNQ 370

Query: 374 ----YSSSNLVLKEVDELSVLLAFSILLNSVQPVLSGVAVGSGWQSYVAYINLGCYYLIG 433
               +SSS  VL  V++LS+LLAF++LLNSVQPVLSGVAVGSGWQSYVAYINLGCYY IG
Sbjct: 371 IAWIFSSSVAVLDAVNKLSLLLAFTVLLNSVQPVLSGVAVGSGWQSYVAYINLGCYYCIG 430

Query: 434 LPLGILIQSFTNLGVKGMWMGMIFGGTGIQTLILLIITSQCDWDGEARKARSHVKKWTD 446
           +PLG L+     LGV G+W GMIFGGT +QT+IL  IT +CDW+ EA+KA + + KW++
Sbjct: 431 VPLGFLMGWGFKLGVMGIWGGMIFGGTAVQTMILSFITMRCDWEKEAQKASARINKWSN 483

BLAST of Spg013281 vs. ExPASy Swiss-Prot
Match: Q1PDX9 (Protein DETOXIFICATION 26 OS=Arabidopsis thaliana OX=3702 GN=DTX26 PE=2 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 3.9e-137
Identity = 247/448 (55.13%), Postives = 318/448 (70.98%), Query Frame = 0

Query: 43  VWIESKKLWHIVGPAIFSRVTSYAVLVLSQASAGHLGDLDLAAFSIAINVIVGLDLGLLM 102
           +WIE+KK+W+IVGP+IF+ + +Y++L+++QA AGHLGDL+LAA SI  N  +G + GLL+
Sbjct: 35  IWIETKKIWYIVGPSIFTGLATYSILIITQAFAGHLGDLELAAISIINNFTLGFNYGLLL 94

Query: 103 GMSSALETLCGQAYGAKKHYMLGVYLQRSWIVLLVCCILLLPVFVFASPILRAIGEPDDL 162
           GM+SALETLCGQA+GA+++YMLGVY+QR WI+L +CCILLLP+++FA+PIL+ IG+ DD+
Sbjct: 95  GMASALETLCGQAFGAREYYMLGVYMQRYWIILFLCCILLLPMYLFATPILKFIGQSDDI 154

Query: 163 AELAGVVARWLIPLHFSFAFYLPLQRFLQSQAKAGAIMWTGVVALVAYAVASWVFVGRLK 222
           AEL G +A W+IP+HF+FAF+ PL RFLQ Q K   I  +  V+L  + +  W FV   K
Sbjct: 155 AELTGTIALWVIPVHFAFAFFFPLNRFLQCQLKNKVIAISAGVSLAVHILVCWFFVYGYK 214

Query: 223 MGVAGIAVACNVAWATMPVILMGYSVCGGCPQTWIGFSVKAFSDLWEFVKLSAASGLMLC 282
           +G+ G   + NV W     IL  YS  GGC  TW GFS +AF+ L E  KLSA+SG+MLC
Sbjct: 215 LGIIGTMASVNVPWWLNIFILFLYSTRGGCTLTWTGFSSEAFTGLLELTKLSASSGIMLC 274

Query: 283 LETWYYRILIMVTGNMKNAEVAVDAVSICMNINGWEMMIPLGFF---------------- 342
           LE WYY+IL+++TGN+ NA++AVD++SICM++NGWEMMIPL FF                
Sbjct: 275 LENWYYKILMLMTGNLVNAKIAVDSLSICMSVNGWEMMIPLAFFAGTGVRVANELGAGNG 334

Query: 343 ----------------VGLF---------------YSSSNLVLKEVDELSVLLAFSILLN 402
                           +GLF               +SSS  VL  VD LSVLLAF++LLN
Sbjct: 335 KGARFATIVSITLSLMIGLFFTVIIVIFHDQIGSIFSSSEAVLNAVDNLSVLLAFTVLLN 394

Query: 403 SVQPVLSGVAVGSGWQSYVAYINLGCYYLIGLPLGILIQSFTNLGVKGMWMGMIFGGTGI 444
           SVQPVLSGVAVGSGWQSYVAYINLGCYYLIGLP G+ +      GVKG+W GMIFGGT I
Sbjct: 395 SVQPVLSGVAVGSGWQSYVAYINLGCYYLIGLPFGLTMGWIFKFGVKGIWAGMIFGGTAI 454

BLAST of Spg013281 vs. ExPASy Swiss-Prot
Match: Q9FNC1 (Protein DETOXIFICATION 28 OS=Arabidopsis thaliana OX=3702 GN=DTX28 PE=3 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 2.8e-135
Identity = 246/460 (53.48%), Postives = 323/460 (70.22%), Query Frame = 0

Query: 32  EDQNCEHIVTRVWIESKKLWHIVGPAIFSRVTSYAVLVLSQASAGHLGDLDLAAFSIAIN 91
           E++N E I   +W+E+KKLW IVGPAIF+RVT+  + V++QA AGHLG+L+LAA SI  N
Sbjct: 27  EEENGE-IKKEIWLETKKLWRIVGPAIFTRVTTNLIFVITQAFAGHLGELELAAISIVNN 86

Query: 92  VIVGLDLGLLMGMSSALETLCGQAYGAKKHYMLGVYLQRSWIVLLVCCILLLPVFVFASP 151
           VI+G +  L +GM++ALETLCGQA+GAKK+ M GVYLQRSWIVL +  ILLLP+++FA+P
Sbjct: 87  VIIGFNYSLFIGMATALETLCGQAFGAKKYDMFGVYLQRSWIVLFLFSILLLPMYIFATP 146

Query: 152 ILRAIGEPDDLAELAGVVARWLIPLHFSFAFYLPLQRFLQSQAKAGAIMWTGVVALVAYA 211
           IL+ +G+PDD+AEL+G+++ W IP HFSFAF+ P+ RFLQ Q K   I  +  V+LV + 
Sbjct: 147 ILKFMGQPDDIAELSGIISVWAIPTHFSFAFFFPINRFLQCQLKNSVIAISSGVSLVVHI 206

Query: 212 VASWVFVGRLKMGVAGIAVACNVAWATMPVILMGYSVCGGCPQTWIGFSVKAFSDLWEFV 271
              W+FV  L++GV G     NV+W     IL  Y+ CGGCP TW GFS+++F+ LWEF 
Sbjct: 207 FVCWLFVYVLELGVIGTIATANVSWWLNVFILFTYTTCGGCPLTWTGFSMESFTRLWEFT 266

Query: 272 KLSAASGLMLCLETWYYRILIMVTGNMKNAEVAVDAVSICMNINGWEMMIPLGFF----- 331
           KLSA+SG+M+CLE WYYR+LI++TGN+++A + VD++SICM+ING EMM+PL FF     
Sbjct: 267 KLSASSGIMVCLENWYYRMLIVMTGNLEDARIDVDSMSICMSINGLEMMVPLAFFAGTSV 326

Query: 332 ------------------------------------------VGLFYSSSNLVLKEVDEL 391
                                                     +G  +SSS  VLK V+ L
Sbjct: 327 RVANELGAGNGKRARFAMIISVTQSLIIGIIISVLIYFLLDQIGWMFSSSETVLKAVNNL 386

Query: 392 SVLLAFSILLNSVQPVLSGVAVGSGWQSYVAYINLGCYYLIGLPLGILIQSFTNLGVKGM 445
           S+LL+F+ILLNSVQPVLSGVAVGSGWQS VA+INLGCYY IGLPLGI++      GVKG+
Sbjct: 387 SILLSFAILLNSVQPVLSGVAVGSGWQSLVAFINLGCYYFIGLPLGIVMGWMFKFGVKGI 446

BLAST of Spg013281 vs. ExPASy Swiss-Prot
Match: Q8W488 (Protein DETOXIFICATION 21 OS=Arabidopsis thaliana OX=3702 GN=DTX21 PE=1 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 6.4e-108
Identity = 212/477 (44.44%), Postives = 303/477 (63.52%), Query Frame = 0

Query: 14  EDSAAIILQPHDHGDGDEEDQNCEHIVTRVWIESKKLWHIVGPAIFSRVTSYAVLVLSQA 73
           E +AA++ +  ++G G+E+D+    +  +VWIESKKLW +  PAIF+R +++ V ++SQ+
Sbjct: 7   ELTAALLKKTAENG-GEEKDE--LGLKQKVWIESKKLWIVAAPAIFTRFSTFGVSIISQS 66

Query: 74  SAGHLGDLDLAAFSIAINVIVGLDLGLLMGMSSALETLCGQAYGAKKHYMLGVYLQRSWI 133
             GHLG ++LAA+SI   V++    G+L+GM+SALETLCGQAYGAK+++MLG+YLQRSWI
Sbjct: 67  FIGHLGPIELAAYSITFTVLLRFSNGILLGMASALETLCGQAYGAKQNHMLGIYLQRSWI 126

Query: 134 VLLVCCILLLPVFVFASPILRAIGEPDDLAELAGVVARWLIPLHFSFAFYLPLQRFLQSQ 193
           VL  C I L PV++F+ PIL A+G+ + +  +A ++A W+I ++FSF      Q FLQ+Q
Sbjct: 127 VLTGCTICLTPVYIFSGPILLALGQEERIVRVARIIALWVIGINFSFVPSFTCQMFLQAQ 186

Query: 194 AKAGAIMWTGVVALVAYAVASWVFVGRLKMGVAGIAVACNVAWATMPVILMGYSVCGGCP 253
           +K   I +   V+L  +   SW+ +     G+ G   +  VA+    +  + +  CGGC 
Sbjct: 187 SKNKIIAYVAAVSLGVHVFLSWLLMVHFNFGITGAMTSTLVAFWLPNIAQLLFVTCGGCK 246

Query: 254 QTWIGFSVKAFSDLWEFVKLSAASGLMLCLETWYYRILIMVTGNMKNAEVAVDAVSICMN 313
            TW GFS+ AF DLW   KLS +SG MLCLE WY  IL+++TGN+KNAEVA+DA++IC+N
Sbjct: 247 DTWRGFSMMAFKDLWPVFKLSMSSGGMLCLELWYNSILVLLTGNLKNAEVALDALAICLN 306

Query: 314 INGWEMMIPLG---------------------------------------FFVGLF---- 373
           ING EMMI LG                                       FFV LF    
Sbjct: 307 INGLEMMIALGFLAAASVRVSNELGSGNPKGAKFATLTAVFTSLSLGIVLFFVFLFLRGR 366

Query: 374 ----YSSSNLVLKEVDELSVLLAFSILLNSVQPVLSGVAVGSGWQSYVAYINLGCYYLIG 433
               +++S  V  EV +LS LLAFSIL+NSVQPVLSGVAVG+GWQ YV Y+NL CYYL+G
Sbjct: 367 VSYIFTTSEAVAAEVADLSPLLAFSILMNSVQPVLSGVAVGAGWQGYVTYVNLACYYLVG 426

Query: 434 LPLGILIQSFTNLGVKGMWMGMIFGGTGIQTLILLIITSQCDWDGEARKARSHVKKW 444
           +P+GI++     L VKG+W+GM+F G  +QT +L ++T + DWD +   +   + +W
Sbjct: 427 IPIGIILGYVVGLQVKGVWIGMLF-GIFVQTCVLTVMTLRTDWDQQVSTSLRRLNRW 479

BLAST of Spg013281 vs. ExPASy Swiss-Prot
Match: F4HPH1 (Protein DETOXIFICATION 22 OS=Arabidopsis thaliana OX=3702 GN=DTX22 PE=2 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 5.1e-105
Identity = 208/477 (43.61%), Postives = 300/477 (62.89%), Query Frame = 0

Query: 14  EDSAAIILQPHDHGDGDEEDQNCEHIVTRVWIESKKLWHIVGPAIFSRVTSYAVLVLSQA 73
           E +AA++ +  ++G G+E D+    +  +VWIESKKLW +  P+IF++ ++Y V +++Q 
Sbjct: 7   ELTAALLKKTTENG-GEENDE--LGLKEKVWIESKKLWVVAAPSIFTKFSTYGVSLVTQG 66

Query: 74  SAGHLGDLDLAAFSIAINVIVGLDLGLLMGMSSALETLCGQAYGAKKHYMLGVYLQRSWI 133
             GH+G  +LAA+SI   V++    G+L+GM+SAL TLCGQAYGAK+++MLG++LQRSWI
Sbjct: 67  FVGHIGPTELAAYSITFTVLLRFSNGILLGMASALGTLCGQAYGAKQYHMLGIHLQRSWI 126

Query: 134 VLLVCCILLLPVFVFASPILRAIGEPDDLAELAGVVARWLIPLHFSFAFYLPLQRFLQSQ 193
           VL  C I ++P+F+F+ PIL A+G+ D +  +A V+A WLI ++F+F      Q FLQSQ
Sbjct: 127 VLTGCTICIMPIFIFSGPILLALGQEDHIVRVARVIALWLIAINFTFVPAFTCQIFLQSQ 186

Query: 194 AKAGAIMWTGVVALVAYAVASWVFVGRLKMGVAGIAVACNVAWATMPVILMGYSVCGGCP 253
           +K   I +   V L  +   SW+ V     G+ G   +  VA+    ++ + Y   GGC 
Sbjct: 187 SKNKIIAYVSAVTLGLHVFFSWLLVVHFNFGITGAMTSTLVAFWMPNIVQLLYVTSGGCK 246

Query: 254 QTWIGFSVKAFSDLWEFVKLSAASGLMLCLETWYYRILIMVTGNMKNAEVAVDAVSICMN 313
            TW GF++ AF DLW   KLS +SG M+CLE WY  IL+++TGN+KNAEVA+DA++IC+N
Sbjct: 247 DTWRGFTMLAFKDLWPVFKLSLSSGGMVCLELWYNSILVLLTGNLKNAEVAIDALAICIN 306

Query: 314 INGWEMMIPLG---------------------------------------FFVGLF---- 373
           +N  +MMI LG                                       FFV LF    
Sbjct: 307 VNALQMMIALGFLAAVSVRVSNELGRGNPEGAKFATIVAVFTSLSIGLVLFFVFLFLRGR 366

Query: 374 ----YSSSNLVLKEVDELSVLLAFSILLNSVQPVLSGVAVGSGWQSYVAYINLGCYYLIG 433
               +++S  V  EV +LS LLAFSILLNSVQPVLSGVAVG+GWQ YVAYINL CYYL+G
Sbjct: 367 ISYIFTTSEAVAAEVADLSPLLAFSILLNSVQPVLSGVAVGAGWQGYVAYINLACYYLLG 426

Query: 434 LPLGILIQSFTNLGVKGMWMGMIFGGTGIQTLILLIITSQCDWDGEARKARSHVKKW 444
           +P+G+++     L VKG+W+GM+F G  +QT +L I+T + DWD +   +  ++ +W
Sbjct: 427 IPVGLVLGYVVGLQVKGVWIGMLF-GIFVQTCVLTIMTLRTDWDQQVSTSLKNINRW 479

BLAST of Spg013281 vs. ExPASy TrEMBL
Match: A0A6J1GFE2 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111453666 PE=3 SV=1)

HSP 1 Score: 638.3 bits (1645), Expect = 2.5e-179
Identity = 320/495 (64.65%), Postives = 384/495 (77.58%), Query Frame = 0

Query: 5   EQHTEFPLLEDSAAIILQPHDHGDGDEEDQNCEHIVTRVWIESKKLWHIVGPAIFSRVTS 64
           EQ    PLL+D +A  LQPH+           + IVTR WIESKKLWHIVGP+IFSR+ S
Sbjct: 3   EQQVNVPLLQDCSA-TLQPHN-----------QDIVTRFWIESKKLWHIVGPSIFSRIVS 62

Query: 65  YAVLVLSQASAGHLGDLDLAAFSIAINVIVGLDLGLLMGMSSALETLCGQAYGAKKHYML 124
           Y++L+++QA AGHL DLDLAAFSIA+ V++G D+GLLMGM+SALETLCGQAYGAKK+YML
Sbjct: 63  YSILIIAQAFAGHLNDLDLAAFSIAVTVVIGFDMGLLMGMASALETLCGQAYGAKKYYML 122

Query: 125 GVYLQRSWIVLLVCCILLLPVFVFASPILRAIGEPDDLAELAGVVARWLIPLHFSFAFYL 184
           G+Y+QRSWIVL +CCILLLP+F FASPIL+ IGEP DLA+LAGV++ W++P+HFSFAFY 
Sbjct: 123 GIYMQRSWIVLFLCCILLLPIFFFASPILKLIGEPHDLADLAGVLSIWMLPIHFSFAFYF 182

Query: 185 PLQRFLQSQAKAGAIMWTGVVALVAYAVASWVFVGRLKMGVAGIAVACNVAWATMPVILM 244
           PLQRFLQSQ K  AI+W+ V ALV++ VASWV V +LKMGV GIAVACNV W  MP++ +
Sbjct: 183 PLQRFLQSQVKVWAIVWSAVAALVSHLVASWVLVVQLKMGVVGIAVACNVGWCAMPIVQI 242

Query: 245 GYSVCGGCPQTWIGFSVKAFSDLWEFVKLSAASGLMLCLETWYYRILIMVTGNMKNAEVA 304
            Y+ CGGCP TW GFSV+AFS LWEFVKLSAASG+MLCLE WYYRIL+++TGNMKNAE+ 
Sbjct: 243 VYTTCGGCPLTWTGFSVEAFSGLWEFVKLSAASGVMLCLENWYYRILVVITGNMKNAEIM 302

Query: 305 VDAVSICMNINGWEMMIPLGFFV------------------------------------- 364
           VDA+SICM+INGWEMMIPLGFFV                                     
Sbjct: 303 VDALSICMSINGWEMMIPLGFFVGTGVRVANELGAGNGEGAKFATIVSSATSLIIGLVFC 362

Query: 365 ----------GLFYSSSNLVLKEVDELSVLLAFSILLNSVQPVLSGVAVGSGWQSYVAYI 424
                     GL +S S +VL+EVD+L++LLA +IL NS+QPVLSGVAVGSGWQSYVAYI
Sbjct: 363 CLIVIFHNSFGLIFSPSAVVLQEVDKLTILLALTILFNSIQPVLSGVAVGSGWQSYVAYI 422

Query: 425 NLGCYYLIGLPLGILIQSFTNLGVKGMWMGMIFGGTGIQTLILLIITSQCDWDGEARKAR 453
           NLGCYY+IGLPLGI +Q FT+LGVKG+W+GMIFGGTGIQTLILL+IT +CDW+GEA+KA 
Sbjct: 423 NLGCYYIIGLPLGIFMQWFTDLGVKGIWIGMIFGGTGIQTLILLLITIRCDWEGEAKKAG 482

BLAST of Spg013281 vs. ExPASy TrEMBL
Match: A0A6J1IT73 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111478439 PE=3 SV=1)

HSP 1 Score: 635.6 bits (1638), Expect = 1.6e-178
Identity = 317/495 (64.04%), Postives = 384/495 (77.58%), Query Frame = 0

Query: 5   EQHTEFPLLEDSAAIILQPHDHGDGDEEDQNCEHIVTRVWIESKKLWHIVGPAIFSRVTS 64
           EQ    PLL+D +A  LQPH+           + IVTR WIESKKLW+IVGP+IFSR+ S
Sbjct: 3   EQQVNVPLLQDCSA-TLQPHN-----------QDIVTRFWIESKKLWNIVGPSIFSRIIS 62

Query: 65  YAVLVLSQASAGHLGDLDLAAFSIAINVIVGLDLGLLMGMSSALETLCGQAYGAKKHYML 124
           Y++L+++QA AGHL DLDLAAFSIA+ V++G D+GLLMGM+SALETLCGQAYGAKK+YML
Sbjct: 63  YSILIIAQAFAGHLNDLDLAAFSIAVTVVIGFDMGLLMGMASALETLCGQAYGAKKYYML 122

Query: 125 GVYLQRSWIVLLVCCILLLPVFVFASPILRAIGEPDDLAELAGVVARWLIPLHFSFAFYL 184
           G+Y+QRSWIVL++CC+LLLP+F FASPIL+ IGEP DLA+LAGV++ W++P+HFSFAFY 
Sbjct: 123 GIYMQRSWIVLILCCVLLLPIFFFASPILKLIGEPHDLADLAGVLSIWMLPIHFSFAFYF 182

Query: 185 PLQRFLQSQAKAGAIMWTGVVALVAYAVASWVFVGRLKMGVAGIAVACNVAWATMPVILM 244
           PLQRFLQSQ K  AI+W+ V ALV++ VASWV V +LKMGV GIAVACN+ W  MP + +
Sbjct: 183 PLQRFLQSQVKVWAIVWSAVAALVSHLVASWVLVVQLKMGVVGIAVACNIGWCAMPTVQI 242

Query: 245 GYSVCGGCPQTWIGFSVKAFSDLWEFVKLSAASGLMLCLETWYYRILIMVTGNMKNAEVA 304
            Y+ CGGCP TW GFSV+AFS LWEFVKLSAASG+MLCLE WYYRIL+++TGNMKNAE+ 
Sbjct: 243 VYTTCGGCPLTWTGFSVEAFSGLWEFVKLSAASGVMLCLENWYYRILVVITGNMKNAEIM 302

Query: 305 VDAVSICMNINGWEMMIPLGFFV------------------------------------- 364
           VDA+SICM+INGWEMMIPLGFFV                                     
Sbjct: 303 VDALSICMSINGWEMMIPLGFFVGTGVRVANELGAGNGEGAKFATIVSSATSLIIGLVFC 362

Query: 365 ----------GLFYSSSNLVLKEVDELSVLLAFSILLNSVQPVLSGVAVGSGWQSYVAYI 424
                     GL +SSS +VL+EVD L++LLA +IL NS+QPVLSGVAVGSGWQSYVAYI
Sbjct: 363 CLIVIFHNSFGLIFSSSAVVLQEVDNLTILLALTILFNSIQPVLSGVAVGSGWQSYVAYI 422

Query: 425 NLGCYYLIGLPLGILIQSFTNLGVKGMWMGMIFGGTGIQTLILLIITSQCDWDGEARKAR 453
           NLGCYY+IGLPLGI +Q FT+LGVKG+W+GMIFGGTGIQTLILL++T +CDW+GEA+KA 
Sbjct: 423 NLGCYYIIGLPLGIFMQWFTDLGVKGIWIGMIFGGTGIQTLILLVVTIRCDWEGEAKKAG 482

BLAST of Spg013281 vs. ExPASy TrEMBL
Match: A0A1S3B3F7 (Protein DETOXIFICATION OS=Cucumis melo OX=3656 GN=LOC103485342 PE=3 SV=1)

HSP 1 Score: 632.9 bits (1631), Expect = 1.0e-177
Identity = 325/491 (66.19%), Postives = 382/491 (77.80%), Query Frame = 0

Query: 5   EQHTEFPLLEDSAAIILQPHDHGDGDEEDQNCEHIVTRVWIESKKLWHIVGPAIFSRVTS 64
           +Q    PLLE+S A ILQ HD     ++DQN E +V RVWIESKKLW+IVGPAI SRV++
Sbjct: 4   DQQISVPLLEESTA-ILQSHD----GDDDQNSEDLVRRVWIESKKLWYIVGPAILSRVST 63

Query: 65  YAVLVLSQASAGHLGDLDLAAFSIAINVIVGLDLGLLMGMSSALETLCGQAYGAKKHYML 124
           ++V+V SQA AGHLGDLDLAA SIA+NVI+G DLGL+MGM+SALETLCGQAYGAKKHYML
Sbjct: 64  HSVMVTSQAFAGHLGDLDLAAISIALNVIIGFDLGLMMGMASALETLCGQAYGAKKHYML 123

Query: 125 GVYLQRSWIVLLVCCILLLPVFVFASPILRAIGEPDDLAELAGVVARWLIPLHFSFAFYL 184
           GVYLQRSW+VL +CC+LLLPVF+FASPIL+AIGE D+LAELAG +ARWLIPLHFSFAFY 
Sbjct: 124 GVYLQRSWMVLFMCCVLLLPVFIFASPILKAIGEGDELAELAGGLARWLIPLHFSFAFYF 183

Query: 185 PLQRFLQSQAKAGAIMWTGVVALVAYAVASWVFVGRLKMGVAGIAVACNVAWATMPVILM 244
           PLQRFLQSQ KA AIMW  VV LV +  ASWVFVG LKMGV GIAVAC+++W  +P+ LM
Sbjct: 184 PLQRFLQSQVKARAIMWVAVVGLVVHVAASWVFVGLLKMGVVGIAVACDISWWVLPIGLM 243

Query: 245 GYSVCGGCPQTWIGFSVKAFSDLWEFVKLSAASGLMLCLETWYYRILIMVTGNMKNAEVA 304
           GYS  GGCP TW GFS++A S LW+F+KLSAASG+MLCLE WYY+ILI++TGNMKNA++ 
Sbjct: 244 GYSAGGGCPNTWTGFSLEALSGLWDFLKLSAASGVMLCLENWYYKILIVMTGNMKNAKIE 303

Query: 305 VDAVSICMNINGWEMMIPLGFFV------------------------------------- 364
           VDA+SICM ING E MIPL FF                                      
Sbjct: 304 VDALSICMGINGLEFMIPLAFFAGTGVRVANELGGGNGKGAKFAAIVSSTTSLVIGLLFC 363

Query: 365 ----------GLFYSSSNLVLKEVDELSVLLAFSILLNSVQPVLSGVAVGSGWQSYVAYI 424
                     GL +SS+++VL+EV++LS+LLAF+IL NSVQPVLSGVAVGSGWQSYVAYI
Sbjct: 364 CLIVIFHDKFGLLFSSTDIVLQEVNKLSILLAFTILFNSVQPVLSGVAVGSGWQSYVAYI 423

Query: 425 NLGCYYLIGLPLGILIQSFTNLGVKGMWMGMIFGGTGIQTLILLIITSQCDWDGEARKAR 449
           NLGCYY IGLPLGI    FT+LGVKG+W+GMIFGGTGIQTLILLIIT +CDW+ EA+KA 
Sbjct: 424 NLGCYYFIGLPLGIFTLRFTDLGVKGIWLGMIFGGTGIQTLILLIITIRCDWEEEAKKAS 483

BLAST of Spg013281 vs. ExPASy TrEMBL
Match: A0A0A0KLT9 (Protein DETOXIFICATION OS=Cucumis sativus OX=3659 GN=Csa_6G499160 PE=3 SV=1)

HSP 1 Score: 632.5 bits (1630), Expect = 1.4e-177
Identity = 325/495 (65.66%), Postives = 381/495 (76.97%), Query Frame = 0

Query: 1   MSEQEQHTEFPLLEDSAAIILQPHDHGDGDEEDQNCEHIVTRVWIESKKLWHIVGPAIFS 60
           M +  Q    PLLE+S   ILQ HD  D    DQN E +V RVWIESKKLW+IVGPAI S
Sbjct: 1   MVDDHQIISVPLLEESTP-ILQSHDRDD----DQNSEDLVRRVWIESKKLWYIVGPAILS 60

Query: 61  RVTSYAVLVLSQASAGHLGDLDLAAFSIAINVIVGLDLGLLMGMSSALETLCGQAYGAKK 120
           RV++++V+V SQA AGHLGDLDLAA SIA+NVI+G DLGL+MGM+SALETLCGQAYGAK+
Sbjct: 61  RVSTHSVMVTSQAFAGHLGDLDLAAISIALNVIIGFDLGLMMGMASALETLCGQAYGAKR 120

Query: 121 HYMLGVYLQRSWIVLLVCCILLLPVFVFASPILRAIGEPDDLAELAGVVARWLIPLHFSF 180
           HYMLGVYLQRSW+VL +CC+LLLPVF+FASPIL+AIGE D+LAELAGV+ARWLIPLHFSF
Sbjct: 121 HYMLGVYLQRSWMVLFMCCVLLLPVFIFASPILKAIGEGDELAELAGVLARWLIPLHFSF 180

Query: 181 AFYLPLQRFLQSQAKAGAIMWTGVVALVAYAVASWVFVGRLKMGVAGIAVACNVAWATMP 240
           AFY PLQRFLQSQ KA AIMW  VV LV +  ASWVFVG LKMGV GIAVAC+++W  +P
Sbjct: 181 AFYFPLQRFLQSQVKARAIMWVAVVGLVVHVAASWVFVGFLKMGVVGIAVACDISWWVLP 240

Query: 241 VILMGYSVCGGCPQTWIGFSVKAFSDLWEFVKLSAASGLMLCLETWYYRILIMVTGNMKN 300
           + LMGYS  GGCP TW GFS++A S LW+F+KLSAASG+MLCLE WYY+ILI++TGNMKN
Sbjct: 241 IGLMGYSAGGGCPYTWTGFSLEALSGLWDFLKLSAASGVMLCLENWYYKILIVMTGNMKN 300

Query: 301 AEVAVDAVSICMNINGWEMMIPLGFFV--------------------------------- 360
           A++ VDA+SICM ING E MIPL FF                                  
Sbjct: 301 AKIEVDALSICMGINGLEFMIPLAFFAGTGVRVANELGGGNGKGAKFAAIVASTTSLVIG 360

Query: 361 --------------GLFYSSSNLVLKEVDELSVLLAFSILLNSVQPVLSGVAVGSGWQSY 420
                         GL +SSS++VL+EV+ LS+LLAF+IL NS+QPVLSGVAVGSGWQSY
Sbjct: 361 LFFCCLIVIFHDKFGLLFSSSDIVLQEVNRLSILLAFTILFNSIQPVLSGVAVGSGWQSY 420

Query: 421 VAYINLGCYYLIGLPLGILIQSFTNLGVKGMWMGMIFGGTGIQTLILLIITSQCDWDGEA 449
           VAYINLGCYY IGLPLGI    FT+LGVKG+W+GMIFGGTGIQT+ILLIIT +CDW+ EA
Sbjct: 421 VAYINLGCYYFIGLPLGIFTLRFTHLGVKGIWLGMIFGGTGIQTMILLIITIRCDWEEEA 480

BLAST of Spg013281 vs. ExPASy TrEMBL
Match: A0A6J1IQ80 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111478447 PE=3 SV=1)

HSP 1 Score: 630.6 bits (1625), Expect = 5.2e-177
Identity = 316/492 (64.23%), Postives = 378/492 (76.83%), Query Frame = 0

Query: 5   EQHTEFPLLEDSAAIILQPHDHGDGDEEDQNCEHIVTRVWIESKKLWHIVGPAIFSRVTS 64
           +  T  PLLEDS  I+     H    ++DQN E IVTRVW+ESKKLW IVGPA+ SR+++
Sbjct: 4   DHQTNVPLLEDSTEIL-----HPGDPDKDQNSEDIVTRVWVESKKLWQIVGPAVLSRIST 63

Query: 65  YAVLVLSQASAGHLGDLDLAAFSIAINVIVGLDLGLLMGMSSALETLCGQAYGAKKHYML 124
           Y+V+V+SQ  AGHLGDLDLAA SIA+NVI+G DLGL+MGM+SALETLCGQAYGAKKHYML
Sbjct: 64  YSVMVISQVFAGHLGDLDLAAISIALNVIIGFDLGLMMGMASALETLCGQAYGAKKHYML 123

Query: 125 GVYLQRSWIVLLVCCILLLPVFVFASPILRAIGEPDDLAELAGVVARWLIPLHFSFAFYL 184
           GVYLQRSW+VL +CC+LLLP  VFAS IL+ IGEPDDLA+LAGV+ARWLIPLHFS AFY 
Sbjct: 124 GVYLQRSWMVLFICCVLLLPALVFASSILKVIGEPDDLADLAGVLARWLIPLHFSLAFYF 183

Query: 185 PLQRFLQSQAKAGAIMWTGVVALVAYAVASWVFVGRLKMGVAGIAVACNVAWATMPVILM 244
           PLQRFLQSQ KA AIMW  +V L  +  ASWVFVG LKMGV GIAVACN++W  +P+ LM
Sbjct: 184 PLQRFLQSQVKARAIMWVAMVGLAVHVAASWVFVGLLKMGVVGIAVACNISWWVLPIGLM 243

Query: 245 GYSVCGGCPQTWIGFSVKAFSDLWEFVKLSAASGLMLCLETWYYRILIMVTGNMKNAEVA 304
            Y+ CGGCP TW GFSV+A + LW+F+KLSAASG+MLCLE WYY+ILI++TGNMKNA++ 
Sbjct: 244 AYTTCGGCPNTWTGFSVEALTGLWDFLKLSAASGVMLCLENWYYKILIVMTGNMKNAKIE 303

Query: 305 VDAVSICMNINGWEMMIPLGFFV------------------------------------- 364
           VDA+SICM+ING E+MIPL FF                                      
Sbjct: 304 VDALSICMSINGLEIMIPLAFFAGTGVRVANELGAGNGKGAKFAAIVSSATSLIIGLVFC 363

Query: 365 ----------GLFYSSSNLVLKEVDELSVLLAFSILLNSVQPVLSGVAVGSGWQSYVAYI 424
                     GL +SSS +VL+EV++LS+LLAF+IL NSVQPVLSGVAVGSGWQSYVAYI
Sbjct: 364 CLIVLLRDKFGLLFSSSAIVLQEVNKLSILLAFTILFNSVQPVLSGVAVGSGWQSYVAYI 423

Query: 425 NLGCYYLIGLPLGILIQSFTNLGVKGMWMGMIFGGTGIQTLILLIITSQCDWDGEARKAR 450
           NLGCYY IGLP+G+    F++LGVKG+WMGMIFGGTGIQTLILLIIT +CDW+GEA+KA 
Sbjct: 424 NLGCYYFIGLPVGMSALKFSHLGVKGIWMGMIFGGTGIQTLILLIITIRCDWEGEAKKAS 483

BLAST of Spg013281 vs. TAIR 10
Match: AT5G65380.1 (MATE efflux family protein )

HSP 1 Score: 511.5 bits (1316), Expect = 6.8e-145
Identity = 262/479 (54.70%), Postives = 337/479 (70.35%), Query Frame = 0

Query: 14  EDSAAIILQPHDHGDGDEEDQNCEHIVTRVWIESKKLWHIVGPAIFSRVTSYAVLVLSQA 73
           E   A++  PH        +++ E +  R+ +E+KKLW IVGPAIFSRVT+Y++LV++QA
Sbjct: 11  ESRVALLKSPH------TAEEDGEGLKDRILVETKKLWQIVGPAIFSRVTTYSMLVITQA 70

Query: 74  SAGHLGDLDLAAFSIAINVIVGLDLGLLMGMSSALETLCGQAYGAKKHYMLGVYLQRSWI 133
            AGHLGDL+LAA SI  NV VG + GLL+GM+SALETLCGQA+GAKK++MLGVY+QRSWI
Sbjct: 71  FAGHLGDLELAAISIVNNVTVGFNFGLLLGMASALETLCGQAFGAKKYHMLGVYMQRSWI 130

Query: 134 VLLVCCILLLPVFVFASPILRAIGEPDDLAELAGVVARWLIPLHFSFAFYLPLQRFLQSQ 193
           VL  CC+LLLP ++F +P+L+ +G+PDD+AEL+GVVA W+IPLHF+F    PLQRFLQ Q
Sbjct: 131 VLFFCCVLLLPTYIFTTPVLKFLGQPDDIAELSGVVAIWVIPLHFAFTLSFPLQRFLQCQ 190

Query: 194 AKAGAIMWTGVVALVAYAVASWVFVGRLKMGVAGIAVACNVAWATMPVILMGYSVCGGCP 253
            K     +   VALV + +  W+FV  LK+GV G     +++W    +IL+ YS CGGCP
Sbjct: 191 LKNRVTAYAAAVALVVHILVCWLFVDGLKLGVVGTVATISISWWVNVLILLVYSTCGGCP 250

Query: 254 QTWIGFSVKAFSDLWEFVKLSAASGLMLCLETWYYRILIMVTGNMKNAEVAVDAVSICMN 313
            TW G S +A + LWEF+KLSA+SG+MLCLE WYYRILI++TGN++NA +AVD++SICM 
Sbjct: 251 LTWTGLSSEALTGLWEFLKLSASSGVMLCLENWYYRILIIMTGNLQNARIAVDSLSICMA 310

Query: 314 INGWEMMIPLGFF--------------------------------VGLF----------- 373
           INGWEMMIPL FF                                +GLF           
Sbjct: 311 INGWEMMIPLAFFAGTGVRVANELGAGNGKGARFATIVSVTQSLIIGLFFWVLIMLLHNQ 370

Query: 374 ----YSSSNLVLKEVDELSVLLAFSILLNSVQPVLSGVAVGSGWQSYVAYINLGCYYLIG 433
               +SSS  VL  V++LS+LLAF++LLNSVQPVLSGVAVGSGWQSYVAYINLGCYY IG
Sbjct: 371 IAWIFSSSVAVLDAVNKLSLLLAFTVLLNSVQPVLSGVAVGSGWQSYVAYINLGCYYCIG 430

Query: 434 LPLGILIQSFTNLGVKGMWMGMIFGGTGIQTLILLIITSQCDWDGEARKARSHVKKWTD 446
           +PLG L+     LGV G+W GMIFGGT +QT+IL  IT +CDW+ EA+KA + + KW++
Sbjct: 431 VPLGFLMGWGFKLGVMGIWGGMIFGGTAVQTMILSFITMRCDWEKEAQKASARINKWSN 483

BLAST of Spg013281 vs. TAIR 10
Match: AT5G10420.1 (MATE efflux family protein )

HSP 1 Score: 489.6 bits (1259), Expect = 2.8e-138
Identity = 247/448 (55.13%), Postives = 318/448 (70.98%), Query Frame = 0

Query: 43  VWIESKKLWHIVGPAIFSRVTSYAVLVLSQASAGHLGDLDLAAFSIAINVIVGLDLGLLM 102
           +WIE+KK+W+IVGP+IF+ + +Y++L+++QA AGHLGDL+LAA SI  N  +G + GLL+
Sbjct: 35  IWIETKKIWYIVGPSIFTGLATYSILIITQAFAGHLGDLELAAISIINNFTLGFNYGLLL 94

Query: 103 GMSSALETLCGQAYGAKKHYMLGVYLQRSWIVLLVCCILLLPVFVFASPILRAIGEPDDL 162
           GM+SALETLCGQA+GA+++YMLGVY+QR WI+L +CCILLLP+++FA+PIL+ IG+ DD+
Sbjct: 95  GMASALETLCGQAFGAREYYMLGVYMQRYWIILFLCCILLLPMYLFATPILKFIGQSDDI 154

Query: 163 AELAGVVARWLIPLHFSFAFYLPLQRFLQSQAKAGAIMWTGVVALVAYAVASWVFVGRLK 222
           AEL G +A W+IP+HF+FAF+ PL RFLQ Q K   I  +  V+L  + +  W FV   K
Sbjct: 155 AELTGTIALWVIPVHFAFAFFFPLNRFLQCQLKNKVIAISAGVSLAVHILVCWFFVYGYK 214

Query: 223 MGVAGIAVACNVAWATMPVILMGYSVCGGCPQTWIGFSVKAFSDLWEFVKLSAASGLMLC 282
           +G+ G   + NV W     IL  YS  GGC  TW GFS +AF+ L E  KLSA+SG+MLC
Sbjct: 215 LGIIGTMASVNVPWWLNIFILFLYSTRGGCTLTWTGFSSEAFTGLLELTKLSASSGIMLC 274

Query: 283 LETWYYRILIMVTGNMKNAEVAVDAVSICMNINGWEMMIPLGFF---------------- 342
           LE WYY+IL+++TGN+ NA++AVD++SICM++NGWEMMIPL FF                
Sbjct: 275 LENWYYKILMLMTGNLVNAKIAVDSLSICMSVNGWEMMIPLAFFAGTGVRVANELGAGNG 334

Query: 343 ----------------VGLF---------------YSSSNLVLKEVDELSVLLAFSILLN 402
                           +GLF               +SSS  VL  VD LSVLLAF++LLN
Sbjct: 335 KGARFATIVSITLSLMIGLFFTVIIVIFHDQIGSIFSSSEAVLNAVDNLSVLLAFTVLLN 394

Query: 403 SVQPVLSGVAVGSGWQSYVAYINLGCYYLIGLPLGILIQSFTNLGVKGMWMGMIFGGTGI 444
           SVQPVLSGVAVGSGWQSYVAYINLGCYYLIGLP G+ +      GVKG+W GMIFGGT I
Sbjct: 395 SVQPVLSGVAVGSGWQSYVAYINLGCYYLIGLPFGLTMGWIFKFGVKGIWAGMIFGGTAI 454

BLAST of Spg013281 vs. TAIR 10
Match: AT5G44050.1 (MATE efflux family protein )

HSP 1 Score: 483.4 bits (1243), Expect = 2.0e-136
Identity = 246/460 (53.48%), Postives = 323/460 (70.22%), Query Frame = 0

Query: 32  EDQNCEHIVTRVWIESKKLWHIVGPAIFSRVTSYAVLVLSQASAGHLGDLDLAAFSIAIN 91
           E++N E I   +W+E+KKLW IVGPAIF+RVT+  + V++QA AGHLG+L+LAA SI  N
Sbjct: 27  EEENGE-IKKEIWLETKKLWRIVGPAIFTRVTTNLIFVITQAFAGHLGELELAAISIVNN 86

Query: 92  VIVGLDLGLLMGMSSALETLCGQAYGAKKHYMLGVYLQRSWIVLLVCCILLLPVFVFASP 151
           VI+G +  L +GM++ALETLCGQA+GAKK+ M GVYLQRSWIVL +  ILLLP+++FA+P
Sbjct: 87  VIIGFNYSLFIGMATALETLCGQAFGAKKYDMFGVYLQRSWIVLFLFSILLLPMYIFATP 146

Query: 152 ILRAIGEPDDLAELAGVVARWLIPLHFSFAFYLPLQRFLQSQAKAGAIMWTGVVALVAYA 211
           IL+ +G+PDD+AEL+G+++ W IP HFSFAF+ P+ RFLQ Q K   I  +  V+LV + 
Sbjct: 147 ILKFMGQPDDIAELSGIISVWAIPTHFSFAFFFPINRFLQCQLKNSVIAISSGVSLVVHI 206

Query: 212 VASWVFVGRLKMGVAGIAVACNVAWATMPVILMGYSVCGGCPQTWIGFSVKAFSDLWEFV 271
              W+FV  L++GV G     NV+W     IL  Y+ CGGCP TW GFS+++F+ LWEF 
Sbjct: 207 FVCWLFVYVLELGVIGTIATANVSWWLNVFILFTYTTCGGCPLTWTGFSMESFTRLWEFT 266

Query: 272 KLSAASGLMLCLETWYYRILIMVTGNMKNAEVAVDAVSICMNINGWEMMIPLGFF----- 331
           KLSA+SG+M+CLE WYYR+LI++TGN+++A + VD++SICM+ING EMM+PL FF     
Sbjct: 267 KLSASSGIMVCLENWYYRMLIVMTGNLEDARIDVDSMSICMSINGLEMMVPLAFFAGTSV 326

Query: 332 ------------------------------------------VGLFYSSSNLVLKEVDEL 391
                                                     +G  +SSS  VLK V+ L
Sbjct: 327 RVANELGAGNGKRARFAMIISVTQSLIIGIIISVLIYFLLDQIGWMFSSSETVLKAVNNL 386

Query: 392 SVLLAFSILLNSVQPVLSGVAVGSGWQSYVAYINLGCYYLIGLPLGILIQSFTNLGVKGM 445
           S+LL+F+ILLNSVQPVLSGVAVGSGWQS VA+INLGCYY IGLPLGI++      GVKG+
Sbjct: 387 SILLSFAILLNSVQPVLSGVAVGSGWQSLVAFINLGCYYFIGLPLGIVMGWMFKFGVKGI 446

BLAST of Spg013281 vs. TAIR 10
Match: AT1G33110.1 (MATE efflux family protein )

HSP 1 Score: 392.5 bits (1007), Expect = 4.6e-109
Identity = 212/477 (44.44%), Postives = 303/477 (63.52%), Query Frame = 0

Query: 14  EDSAAIILQPHDHGDGDEEDQNCEHIVTRVWIESKKLWHIVGPAIFSRVTSYAVLVLSQA 73
           E +AA++ +  ++G G+E+D+    +  +VWIESKKLW +  PAIF+R +++ V ++SQ+
Sbjct: 7   ELTAALLKKTAENG-GEEKDE--LGLKQKVWIESKKLWIVAAPAIFTRFSTFGVSIISQS 66

Query: 74  SAGHLGDLDLAAFSIAINVIVGLDLGLLMGMSSALETLCGQAYGAKKHYMLGVYLQRSWI 133
             GHLG ++LAA+SI   V++    G+L+GM+SALETLCGQAYGAK+++MLG+YLQRSWI
Sbjct: 67  FIGHLGPIELAAYSITFTVLLRFSNGILLGMASALETLCGQAYGAKQNHMLGIYLQRSWI 126

Query: 134 VLLVCCILLLPVFVFASPILRAIGEPDDLAELAGVVARWLIPLHFSFAFYLPLQRFLQSQ 193
           VL  C I L PV++F+ PIL A+G+ + +  +A ++A W+I ++FSF      Q FLQ+Q
Sbjct: 127 VLTGCTICLTPVYIFSGPILLALGQEERIVRVARIIALWVIGINFSFVPSFTCQMFLQAQ 186

Query: 194 AKAGAIMWTGVVALVAYAVASWVFVGRLKMGVAGIAVACNVAWATMPVILMGYSVCGGCP 253
           +K   I +   V+L  +   SW+ +     G+ G   +  VA+    +  + +  CGGC 
Sbjct: 187 SKNKIIAYVAAVSLGVHVFLSWLLMVHFNFGITGAMTSTLVAFWLPNIAQLLFVTCGGCK 246

Query: 254 QTWIGFSVKAFSDLWEFVKLSAASGLMLCLETWYYRILIMVTGNMKNAEVAVDAVSICMN 313
            TW GFS+ AF DLW   KLS +SG MLCLE WY  IL+++TGN+KNAEVA+DA++IC+N
Sbjct: 247 DTWRGFSMMAFKDLWPVFKLSMSSGGMLCLELWYNSILVLLTGNLKNAEVALDALAICLN 306

Query: 314 INGWEMMIPLG---------------------------------------FFVGLF---- 373
           ING EMMI LG                                       FFV LF    
Sbjct: 307 INGLEMMIALGFLAAASVRVSNELGSGNPKGAKFATLTAVFTSLSLGIVLFFVFLFLRGR 366

Query: 374 ----YSSSNLVLKEVDELSVLLAFSILLNSVQPVLSGVAVGSGWQSYVAYINLGCYYLIG 433
               +++S  V  EV +LS LLAFSIL+NSVQPVLSGVAVG+GWQ YV Y+NL CYYL+G
Sbjct: 367 VSYIFTTSEAVAAEVADLSPLLAFSILMNSVQPVLSGVAVGAGWQGYVTYVNLACYYLVG 426

Query: 434 LPLGILIQSFTNLGVKGMWMGMIFGGTGIQTLILLIITSQCDWDGEARKARSHVKKW 444
           +P+GI++     L VKG+W+GM+F G  +QT +L ++T + DWD +   +   + +W
Sbjct: 427 IPIGIILGYVVGLQVKGVWIGMLF-GIFVQTCVLTVMTLRTDWDQQVSTSLRRLNRW 479

BLAST of Spg013281 vs. TAIR 10
Match: AT1G33090.1 (MATE efflux family protein )

HSP 1 Score: 382.9 bits (982), Expect = 3.6e-106
Identity = 208/477 (43.61%), Postives = 300/477 (62.89%), Query Frame = 0

Query: 14  EDSAAIILQPHDHGDGDEEDQNCEHIVTRVWIESKKLWHIVGPAIFSRVTSYAVLVLSQA 73
           E +AA++ +  ++G G+E D+    +  +VWIESKKLW +  P+IF++ ++Y V +++Q 
Sbjct: 7   ELTAALLKKTTENG-GEENDE--LGLKEKVWIESKKLWVVAAPSIFTKFSTYGVSLVTQG 66

Query: 74  SAGHLGDLDLAAFSIAINVIVGLDLGLLMGMSSALETLCGQAYGAKKHYMLGVYLQRSWI 133
             GH+G  +LAA+SI   V++    G+L+GM+SAL TLCGQAYGAK+++MLG++LQRSWI
Sbjct: 67  FVGHIGPTELAAYSITFTVLLRFSNGILLGMASALGTLCGQAYGAKQYHMLGIHLQRSWI 126

Query: 134 VLLVCCILLLPVFVFASPILRAIGEPDDLAELAGVVARWLIPLHFSFAFYLPLQRFLQSQ 193
           VL  C I ++P+F+F+ PIL A+G+ D +  +A V+A WLI ++F+F      Q FLQSQ
Sbjct: 127 VLTGCTICIMPIFIFSGPILLALGQEDHIVRVARVIALWLIAINFTFVPAFTCQIFLQSQ 186

Query: 194 AKAGAIMWTGVVALVAYAVASWVFVGRLKMGVAGIAVACNVAWATMPVILMGYSVCGGCP 253
           +K   I +   V L  +   SW+ V     G+ G   +  VA+    ++ + Y   GGC 
Sbjct: 187 SKNKIIAYVSAVTLGLHVFFSWLLVVHFNFGITGAMTSTLVAFWMPNIVQLLYVTSGGCK 246

Query: 254 QTWIGFSVKAFSDLWEFVKLSAASGLMLCLETWYYRILIMVTGNMKNAEVAVDAVSICMN 313
            TW GF++ AF DLW   KLS +SG M+CLE WY  IL+++TGN+KNAEVA+DA++IC+N
Sbjct: 247 DTWRGFTMLAFKDLWPVFKLSLSSGGMVCLELWYNSILVLLTGNLKNAEVAIDALAICIN 306

Query: 314 INGWEMMIPLG---------------------------------------FFVGLF---- 373
           +N  +MMI LG                                       FFV LF    
Sbjct: 307 VNALQMMIALGFLAAVSVRVSNELGRGNPEGAKFATIVAVFTSLSIGLVLFFVFLFLRGR 366

Query: 374 ----YSSSNLVLKEVDELSVLLAFSILLNSVQPVLSGVAVGSGWQSYVAYINLGCYYLIG 433
               +++S  V  EV +LS LLAFSILLNSVQPVLSGVAVG+GWQ YVAYINL CYYL+G
Sbjct: 367 ISYIFTTSEAVAAEVADLSPLLAFSILLNSVQPVLSGVAVGAGWQGYVAYINLACYYLLG 426

Query: 434 LPLGILIQSFTNLGVKGMWMGMIFGGTGIQTLILLIITSQCDWDGEARKARSHVKKW 444
           +P+G+++     L VKG+W+GM+F G  +QT +L I+T + DWD +   +  ++ +W
Sbjct: 427 IPVGLVLGYVVGLQVKGVWIGMLF-GIFVQTCVLTIMTLRTDWDQQVSTSLKNINRW 479

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022950623.15.1e-17964.65protein DETOXIFICATION 27-like isoform X2 [Cucurbita moschata][more]
XP_023544211.12.6e-17864.44protein DETOXIFICATION 27-like isoform X3 [Cucurbita pepo subsp. pepo][more]
XP_022978468.13.3e-17864.04protein DETOXIFICATION 27-like isoform X2 [Cucurbita maxima][more]
KAG6604053.12.2e-17764.63Protein DETOXIFICATION 27, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_008441115.12.2e-17766.19PREDICTED: protein DETOXIFICATION 27-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
Q9FKQ19.5e-14454.70Protein DETOXIFICATION 27 OS=Arabidopsis thaliana OX=3702 GN=DTX27 PE=2 SV=1[more]
Q1PDX93.9e-13755.13Protein DETOXIFICATION 26 OS=Arabidopsis thaliana OX=3702 GN=DTX26 PE=2 SV=1[more]
Q9FNC12.8e-13553.48Protein DETOXIFICATION 28 OS=Arabidopsis thaliana OX=3702 GN=DTX28 PE=3 SV=1[more]
Q8W4886.4e-10844.44Protein DETOXIFICATION 21 OS=Arabidopsis thaliana OX=3702 GN=DTX21 PE=1 SV=1[more]
F4HPH15.1e-10543.61Protein DETOXIFICATION 22 OS=Arabidopsis thaliana OX=3702 GN=DTX22 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1GFE22.5e-17964.65Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111453666 PE=3 SV=1[more]
A0A6J1IT731.6e-17864.04Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111478439 PE=3 SV=1[more]
A0A1S3B3F71.0e-17766.19Protein DETOXIFICATION OS=Cucumis melo OX=3656 GN=LOC103485342 PE=3 SV=1[more]
A0A0A0KLT91.4e-17765.66Protein DETOXIFICATION OS=Cucumis sativus OX=3659 GN=Csa_6G499160 PE=3 SV=1[more]
A0A6J1IQ805.2e-17764.23Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111478447 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G65380.16.8e-14554.70MATE efflux family protein [more]
AT5G10420.12.8e-13855.13MATE efflux family protein [more]
AT5G44050.12.0e-13653.48MATE efflux family protein [more]
AT1G33110.14.6e-10944.44MATE efflux family protein [more]
AT1G33090.13.6e-10643.61MATE efflux family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002528Multi antimicrobial extrusion proteinPFAMPF01554MatEcoord: 56..214
e-value: 5.4E-28
score: 97.7
NoneNo IPR availablePANTHERPTHR11206:SF194PROTEIN DETOXIFICATIONcoord: 29..328
coord: 328..439
NoneNo IPR availablePANTHERPTHR11206MULTIDRUG RESISTANCE PROTEINcoord: 29..328
coord: 328..439
IPR045069Multidrug and toxic compound extrusion family, eukaryoticCDDcd13132MATE_eukaryoticcoord: 46..435
e-value: 3.43852E-117
score: 348.408

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg013281.1Spg013281.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1990961 xenobiotic detoxification by transmembrane export across the plasma membrane
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016020 membrane
molecular_function GO:0015297 antiporter activity
molecular_function GO:0042910 xenobiotic transmembrane transporter activity