Spg013218 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg013218
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	RING-type E3 ubiquitin transferase
Location	scaffold1: 17068656 .. 17071607 (+)
RNA-Seq Expression	Spg013218
Synteny	Spg013218

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTCAAACCTATTTACAACTTCCCAACAACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTTCAAACCCTAGCTTCCATTGTTTCTCTCTCCCATGGCAGAAGCAGATCAACCAGTTGATCAAGTCGATCCTCCTCCCTTCTTTCTCTGTCCCATCTCTCTGCAAATCATGAAGGATCCCGTCACGGTCGCCTCCGGCATGACCTACGACCGGGAGAACATCGAGAGATGGTTGTTCTCCGACAAGCACAAGACGTGTCCGGTCACGCAGGTGGTCTTGTCGGACTTCGGTCTTACTCCCAACCACACTCTCCGACGTGTCATTCAAGCTTGGTGCACCGTCAATGGTGGGATCGAGAGGATTCCGACCCCGAAGGCTCCGGTCGACCGGAAGCAGGTCGTTGAGATTCTGGCGGAGGCGAAGCTGTCTCCGTTCTCTCAGCAGAATTGTCTCCGGCGTCTTCGATCCATTGCGTCGGCGAGTGAGACCAATAAACGTTGCATGGAGGCTGCCGGAGTTGTGGAGTTCTTGGTTGGAGTTGTGTGTAATTCCATCAATGTTGTTGATTCGAGCTTTGAAGGTATATACTACTAAACTCTTAATTTTAAGCTAATTATAGGTTTAGGGCGTTTAATTTAACCTTTCATACACATTATAAGTTTTCAAAATATTAGAAAACTCATTGAATAAACTATTTCATGAAACGAGAAAGAATTAAGGTCTCGTTTGATAAACATTTGGTTTTTGAAAATTAGGGTCCGTTTGGAATACATTCTAATTTAAATGTTTTTCAAGAATGCATTTTTTAAAAAAACATTTTGTATAAAAAATGTTTAGAATTTAAATACTCATATGTTTGGTTGCATTTCCTCCTAGGTGTTTTTATACATAAGTTATTGTATATTAAAGTCTCTTTTTAAAAAAGTTGGTTTCAAGTATTTCTCTCAAAATGGTTTATTTATCAATCTCATTTTCTAATAAGTCATTTTTAGTGGTTGCCAAACACTATAAATTTTTTCAAAATGATTTTTTTTCAAAATTAAACACTTGAAAAGTTAAACCAAACACACCCTAGACTTGTTTTCTTTAAATTTCTTCATAATGGTTTACATTTTTGTTAAAGACACCCTTGAATTCCTAACCAAATTCTAAAAACAAAAAAAAAAAATTTGAAAACTACTTTTTTTTAGTTTTCAAAAATTGGCTTGGTTTTTGAAAACATGGGAAGAAGGGTAGGTGAAAAGCCAATGAAACTTAGAGTTGGAAGTGGTATGTTTATAAGCTTAGAAACCAAAAACCAAATGGTTATCAAACGGGACCTAAACAATAAATTAGGAATTCAAATCTAGGACTTTTAATTAATTATACGATATTGTGATATATTATATTAAACTATCACTAAATTAAAAACTTTAGCTAATAGTTAGTGTTAGGACACATAAATTTAATATTTTATTCATATTTCTAACACACTCTTTAAACATTTCTTACTCTAATATAGCATGTTAAATTTATAGATATATTAATTATTATACACTCTTTACATTTATTACTTTGATATCATGCTAAATTATCGTTAAACTTAAATGTTTAAATTAATATGTTAGAATTAATATATTAGGATAAATTTGTAGTATTAATACTATCTCTTCATCGATCTGAATATAGCTCATTAGATAAGACATCAGAACACTAATTATTATTTTAAAAGGTGGATGACTTGATCTCCCATTCCGAAATATAAATAAATAAATAATAAATAAATAATTATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCTTTCAAAAACTATCTTATGAAATTGGAGACAATTTTTTTTTTTTTTTAAATATTGAATATAGGATTTCTTCTAATAAGTTAGCATGGAATTTAAAATTTTATGAATAATGATGACGCAGATTACGCGTTTGACGACCAGTTAGCAAATTTGGGCGATGAGGCTTTGAGCATTCTTTACAAACTTCAAATCTCCGAATCCGGCCTCAAATCTCTTCTCGCCAATAATGGCGGCGTCTTCCTCACAACCCTGACCAAAATCTTGCAGAACGGCACCTACGGCTCCCGAGCGTATGCAGTAATGCTTCTTCAATCAATGTTCGAAGTCGCCGATCAGATCCAAATCCTCAGCCTAAACGCCGATTTCTTCATCGAAATCGTCCAAATTCTGAAGGATCAGATCTCGAAACAGGCTTCCAAATCGGCATTGAAACTGCTAATCGGACTCTGTTCGAGTTCCAGAAACAGAGTCAAGGCGGTCCAAGCCGGTGCGGTTCCGGTTCTGATCGATCTCCTCCTCGATCTCGACTCGTCGGAGAAGCGTCTCTGCGAGATGATTCTCGTCGCGCTCGATCTGCTCTGCGGCTGCGCCGACGGCAGGGCGGAGCTGTTGAACCACGCGGCGGGGATGGCCGTCGTGTCGAAGAAGATTCTCAGAGTTTCGTCGCTCGGAAGCGAGAAGGCCGTCGGAATTTTGTGCTCCGTCGCGAGATACTCGGCGAGCCAAACCGTCGTTCAGGAAATGGTGCGGCTCGGAGTTGTGACGAAGCTCTGTTTTCTGCTTCAAGTCGTCGGCGTTGGAGCCAAAGCGAAAGAGAAGGCCAGAGAGATTCTGAAGCTCCATGGTCGGACATGGCGGAATTCTTCATGTTTGCCTTCGAGTTTGCGATCTTCTTATCCACTTTAGGGGTTTTTTTTTCCCAAAACTTCTATTCTTTCTTTCATTTCTTTCAAATGCATATAGTTGAGTCGATTGAAATTTGATTGTGTAGTTCAAAAATTTGTACATATTCTTTTGTAAATAATTTGTTGAATTCGGATTGGCTCGAGTGTAATTCAAGTAATCGGGCCTTTTGTAAGCTGTAGGTTTGAATTTTCTTTCACTTTGATGTAATATTGTGAAAAAAAATTGTCAT

mRNA sequence

ATGGCAGAAGCAGATCAACCAGTTGATCAAGTCGATCCTCCTCCCTTCTTTCTCTGTCCCATCTCTCTGCAAATCATGAAGGATCCCGTCACGGTCGCCTCCGGCATGACCTACGACCGGGAGAACATCGAGAGATGGTTGTTCTCCGACAAGCACAAGACGTGTCCGGTCACGCAGGTGGTCTTGTCGGACTTCGGTCTTACTCCCAACCACACTCTCCGACGTGTCATTCAAGCTTGGTGCACCGTCAATGGTGGGATCGAGAGGATTCCGACCCCGAAGGCTCCGGTCGACCGGAAGCAGGTCGTTGAGATTCTGGCGGAGGCGAAGCTGTCTCCGTTCTCTCAGCAGAATTGTCTCCGGCGTCTTCGATCCATTGCGTCGGCGAGTGAGACCAATAAACGTTGCATGGAGGCTGCCGGAGTTGTGGAGTTCTTGGTTGGAGTTGTGTGTAATTCCATCAATGTTGTTGATTCGAGCTTTGAAGATTACGCGTTTGACGACCAGTTAGCAAATTTGGGCGATGAGGCTTTGAGCATTCTTTACAAACTTCAAATCTCCGAATCCGGCCTCAAATCTCTTCTCGCCAATAATGGCGGCGTCTTCCTCACAACCCTGACCAAAATCTTGCAGAACGGCACCTACGGCTCCCGAGCGTATGCAGTAATGCTTCTTCAATCAATGTTCGAAGTCGCCGATCAGATCCAAATCCTCAGCCTAAACGCCGATTTCTTCATCGAAATCGTCCAAATTCTGAAGGATCAGATCTCGAAACAGGCTTCCAAATCGGCATTGAAACTGCTAATCGGACTCTGTTCGAGTTCCAGAAACAGAGTCAAGGCGGTCCAAGCCGGTGCGGTTCCGGTTCTGATCGATCTCCTCCTCGATCTCGACTCGTCGGAGAAGCGTCTCTGCGAGATGATTCTCGTCGCGCTCGATCTGCTCTGCGGCTGCGCCGACGGCAGGGCGGAGCTGTTGAACCACGCGGCGGGGATGGCCGTCGTGTCGAAGAAGATTCTCAGAGTTTCGTCGCTCGGAAGCGAGAAGGCCGTCGGAATTTTGTGCTCCGTCGCGAGATACTCGGCGAGCCAAACCGTCGTTCAGGAAATGGTGCGGCTCGGAGTTGTGACGAAGCTCTGTTTTCTGCTTCAAGTCGTCGGCGTTGGAGCCAAAGCGAAAGAGAAGGCCAGAGAGATTCTGAAGCTCCATGGTCGGACATGGCGGAATTCTTCATGTTTGCCTTCGAGTTTGCGATCTTCTTATCCACTTTAG

Coding sequence (CDS)

ATGGCAGAAGCAGATCAACCAGTTGATCAAGTCGATCCTCCTCCCTTCTTTCTCTGTCCCATCTCTCTGCAAATCATGAAGGATCCCGTCACGGTCGCCTCCGGCATGACCTACGACCGGGAGAACATCGAGAGATGGTTGTTCTCCGACAAGCACAAGACGTGTCCGGTCACGCAGGTGGTCTTGTCGGACTTCGGTCTTACTCCCAACCACACTCTCCGACGTGTCATTCAAGCTTGGTGCACCGTCAATGGTGGGATCGAGAGGATTCCGACCCCGAAGGCTCCGGTCGACCGGAAGCAGGTCGTTGAGATTCTGGCGGAGGCGAAGCTGTCTCCGTTCTCTCAGCAGAATTGTCTCCGGCGTCTTCGATCCATTGCGTCGGCGAGTGAGACCAATAAACGTTGCATGGAGGCTGCCGGAGTTGTGGAGTTCTTGGTTGGAGTTGTGTGTAATTCCATCAATGTTGTTGATTCGAGCTTTGAAGATTACGCGTTTGACGACCAGTTAGCAAATTTGGGCGATGAGGCTTTGAGCATTCTTTACAAACTTCAAATCTCCGAATCCGGCCTCAAATCTCTTCTCGCCAATAATGGCGGCGTCTTCCTCACAACCCTGACCAAAATCTTGCAGAACGGCACCTACGGCTCCCGAGCGTATGCAGTAATGCTTCTTCAATCAATGTTCGAAGTCGCCGATCAGATCCAAATCCTCAGCCTAAACGCCGATTTCTTCATCGAAATCGTCCAAATTCTGAAGGATCAGATCTCGAAACAGGCTTCCAAATCGGCATTGAAACTGCTAATCGGACTCTGTTCGAGTTCCAGAAACAGAGTCAAGGCGGTCCAAGCCGGTGCGGTTCCGGTTCTGATCGATCTCCTCCTCGATCTCGACTCGTCGGAGAAGCGTCTCTGCGAGATGATTCTCGTCGCGCTCGATCTGCTCTGCGGCTGCGCCGACGGCAGGGCGGAGCTGTTGAACCACGCGGCGGGGATGGCCGTCGTGTCGAAGAAGATTCTCAGAGTTTCGTCGCTCGGAAGCGAGAAGGCCGTCGGAATTTTGTGCTCCGTCGCGAGATACTCGGCGAGCCAAACCGTCGTTCAGGAAATGGTGCGGCTCGGAGTTGTGACGAAGCTCTGTTTTCTGCTTCAAGTCGTCGGCGTTGGAGCCAAAGCGAAAGAGAAGGCCAGAGAGATTCTGAAGCTCCATGGTCGGACATGGCGGAATTCTTCATGTTTGCCTTCGAGTTTGCGATCTTCTTATCCACTTTAG

Protein sequence

MAEADQPVDQVDPPPFFLCPISLQIMKDPVTVASGMTYDRENIERWLFSDKHKTCPVTQVVLSDFGLTPNHTLRRVIQAWCTVNGGIERIPTPKAPVDRKQVVEILAEAKLSPFSQQNCLRRLRSIASASETNKRCMEAAGVVEFLVGVVCNSINVVDSSFEDYAFDDQLANLGDEALSILYKLQISESGLKSLLANNGGVFLTTLTKILQNGTYGSRAYAVMLLQSMFEVADQIQILSLNADFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAVPVLIDLLLDLDSSEKRLCEMILVALDLLCGCADGRAELLNHAAGMAVVSKKILRVSSLGSEKAVGILCSVARYSASQTVVQEMVRLGVVTKLCFLLQVVGVGAKAKEKAREILKLHGRTWRNSSCLPSSLRSSYPL

Homology

[Close]

BLAST of Spg013218 vs. NCBI nr
Match: XP_022925148.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita moschata])

HSP 1 Score: 706.4 bits (1822), Expect = 1.4e-199
Identity = 364/413 (88.14%), Postives = 387/413 (93.70%), Query Frame = 0


Query: 10  QVDPPPFFLCPISLQIMKDPVTVASGMTYDRENIERWLFSDKHKTCPVTQVVLSDFGLTP 69
           +VDPPPFFLCPISLQIMKDPVTVASG+TYDRENIERWLFSDKH TCPVTQVVLSDF +TP
Sbjct: 3   EVDPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLFSDKHTTCPVTQVVLSDFDITP 62

Query: 70  NHTLRRVIQAWCTVNGGIERIPTPKAPVDRKQVVEILAEAKLSPFSQQNCLRRLRSIASA 129
           N+TL R+IQAWCTVN  +ERIPTPK PVD+KQVV+ILA+ KLSPFSQQNCLRRL SIASA
Sbjct: 63  NNTLSRLIQAWCTVNNRVERIPTPKPPVDKKQVVKILADVKLSPFSQQNCLRRLESIASA 122

Query: 130 SETNKRCMEAAGVVEFLVGVVCNSINVVDSSFEDYAFDDQLANLGDEALSILYKLQISES 189
           +E NKRCMEAAG VEFLVGVVCNSI  ++SSF D+AFDD LANLGDEALSILYKLQISES
Sbjct: 123 NEMNKRCMEAAGAVEFLVGVVCNSI-TIESSFPDFAFDDPLANLGDEALSILYKLQISES 182

Query: 190 GLKSLLANNGGVFLTTLTKILQNGTYGSRAYAVMLLQSMFEVADQIQILSLNADFFIEIV 249
           GLK LLANNGG+F+TTLTK LQNGTYGSRAYA MLLQSMFEVAD+IQI SLN +FF+EIV
Sbjct: 183 GLKFLLANNGGIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIRSLNLEFFVEIV 242

Query: 250 QILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAVPVLIDLLLDLDSSEKRLCEMIL 309
           QILKDQISKQASKSALKLLIGLCSS RNRV AVQAGAVPVLIDLLLDLDSSEKRLCEMIL
Sbjct: 243 QILKDQISKQASKSALKLLIGLCSSFRNRVNAVQAGAVPVLIDLLLDLDSSEKRLCEMIL 302

Query: 310 VALDLLCGCADGRAELLNHAAGMAVVSKKILRVSSLGSEKAVGILCSVARYSASQTVVQE 369
           VALDLLCGCADGRAELLNHAAG+AVVSKKILRVSSLGSEKAVGILCS+AR+SASQ+VVQE
Sbjct: 303 VALDLLCGCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSVVQE 362

Query: 370 MVRLGVVTKLCFLLQVVGVGAKAKEKAREILKLHGRTWRNSSCLPSSLRSSYP 423
           M RLGVV KLCFLLQVVG GAKAKEKA+EILKLHGRTWRNSSC PSSLR SYP
Sbjct: 363 MARLGVVVKLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPSSLRPSYP 414

[Close]

BLAST of Spg013218 vs. NCBI nr
Match: XP_022978192.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita maxima])

HSP 1 Score: 704.5 bits (1817), Expect = 5.5e-199
Identity = 367/426 (86.15%), Postives = 397/426 (93.19%), Query Frame = 0


Query: 1   MAEADQPVDQVDPPPFFLCPISLQIMKDPVTVASGMTYDRENIERWLFSDKHKTCPVTQV 60
           MAE DQ   ++DPPPFFLCPISLQIMKDPVTVASG+TYDRE+IERWLFSD+HKTCPVTQV
Sbjct: 1   MAELDQ---ELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQV 60

Query: 61  VLSDFGLTPNHTLRRVIQAWCTVNG--GIERIPTPKAPVDRKQVVEILAEAKLSPFSQQN 120
           VLS F +TPNHT+RRVIQAWCT+N   GIERIPTPK P+D KQVV ILA+AKLSPFSQQN
Sbjct: 61  VLSSFDITPNHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAKLSPFSQQN 120

Query: 121 CLRRLRSIASASETNKRCMEAAGVVEFLVGVVCNSINVVDSSFEDYAF--DDQLANLGDE 180
           CLRRLRSIA+ASE+NKRCMEAAG VEFL G+VCNS N V+ S EDYA+  DDQ ANLGDE
Sbjct: 121 CLRRLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTN-VEFSLEDYAYLIDDQFANLGDE 180

Query: 181 ALSILYKLQISESGLKSLLANNGGVFLTTLTKILQNGTYGSRAYAVMLLQSMFEVADQIQ 240
           ALSILYKLQISESGLK LLANNGG+F++TLTKILQNGTYGSRAY+VMLL SMFEVADQIQ
Sbjct: 181 ALSILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQ 240

Query: 241 ILSLNADFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAVPVLIDLLLD 300
           ILSLN DFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGA+PVLIDLLLD
Sbjct: 241 ILSLNTDFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLIDLLLD 300

Query: 301 LDSSEKRLCEMILVALDLLCGCADGRAELLNHAAGMAVVSKKILRVSSLGSEKAVGILCS 360
           LDSSEKR CEMILV LDLLC CADGRAE+LNHAAG+AVVSKKILRVS+LGSEKAVGILCS
Sbjct: 301 LDSSEKRSCEMILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCS 360

Query: 361 VARYSASQTVVQEMVRLGVVTKLCFLLQVVGVGAKAKEKAREILKLHGRTWRNSSCLPSS 420
           +AR+SAS++VVQEM+RLGVVTKLCFLLQVVG G KAKEKA+EILKLHGRTWRNSSCLPSS
Sbjct: 361 IARFSASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSS 420

Query: 421 LRSSYP 423
           LRSS+P
Sbjct: 421 LRSSFP 422

[Close]

BLAST of Spg013218 vs. NCBI nr
Match: XP_023543358.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 703.7 bits (1815), Expect = 9.3e-199
Identity = 368/426 (86.38%), Postives = 397/426 (93.19%), Query Frame = 0


Query: 1   MAEADQPVDQVDPPPFFLCPISLQIMKDPVTVASGMTYDRENIERWLFSDKHKTCPVTQV 60
           MAE DQ   ++DPPPFFLCPISLQIMKDPVTVASG+TYDRE+IE+WLFSD+HKTCPVTQV
Sbjct: 1   MAELDQ---ELDPPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDRHKTCPVTQV 60

Query: 61  VLSDFGLTPNHTLRRVIQAWCTVNG--GIERIPTPKAPVDRKQVVEILAEAKLSPFSQQN 120
           VLS F +TPNHTLRRVIQAWCTVN   GIERIPTPK PVD KQVV ILA+AKLSPFSQQN
Sbjct: 61  VLSSFDITPNHTLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQN 120

Query: 121 CLRRLRSIASASETNKRCMEAAGVVEFLVGVVCNSINVVDSSFEDYAF--DDQLANLGDE 180
           CLRRLRSIA+ SE+NKRCMEAAG VEFL G+VCNS NV  SS EDYA+  DDQ ANLGDE
Sbjct: 121 CLRRLRSIAAGSESNKRCMEAAGAVEFLAGIVCNSTNVEFSS-EDYAYLIDDQFANLGDE 180

Query: 181 ALSILYKLQISESGLKSLLANNGGVFLTTLTKILQNGTYGSRAYAVMLLQSMFEVADQIQ 240
           ALSILYKLQISESGLK LLANNGG+F++TLTKILQNGTYGSRAY+VMLL SMFEVADQIQ
Sbjct: 181 ALSILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQ 240

Query: 241 ILSLNADFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAVPVLIDLLLD 300
           ILSLN DFF+EIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAV+AGAVPV+IDLLLD
Sbjct: 241 ILSLNTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVMIDLLLD 300

Query: 301 LDSSEKRLCEMILVALDLLCGCADGRAELLNHAAGMAVVSKKILRVSSLGSEKAVGILCS 360
           LDSSEKR CEMILV LDLLCGCADGRAE+LNHAAG+AVVSKKILRVS+LGSEKAVGILCS
Sbjct: 301 LDSSEKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCS 360

Query: 361 VARYSASQTVVQEMVRLGVVTKLCFLLQVVGVGAKAKEKAREILKLHGRTWRNSSCLPSS 420
           +AR+SAS++VVQEM+RLGVVTKLCFLLQVVG G KAKEKA+EILKLHGRTWRNSSCLPSS
Sbjct: 361 IARFSASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSS 420

Query: 421 LRSSYP 423
           LRSS+P
Sbjct: 421 LRSSFP 422

[Close]

BLAST of Spg013218 vs. NCBI nr
Match: XP_022950790.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita moschata])

HSP 1 Score: 697.2 bits (1798), Expect = 8.7e-197
Identity = 363/426 (85.21%), Postives = 396/426 (92.96%), Query Frame = 0


Query: 1   MAEADQPVDQVDPPPFFLCPISLQIMKDPVTVASGMTYDRENIERWLFSDKHKTCPVTQV 60
           MAE DQ   ++DPPPFFLCPISLQIMKDPVTVASG+TYDRE+IERWLFSD+HKTCP+TQV
Sbjct: 1   MAELDQ---ELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPITQV 60

Query: 61  VLSDFGLTPNHTLRRVIQAWCTVNG--GIERIPTPKAPVDRKQVVEILAEAKLSPFSQQN 120
           VLS+F +TPNHTLRRVIQAWCTVN   G+ERIPTPK PVD KQVV ILA+AKLS FSQQN
Sbjct: 61  VLSNFDITPNHTLRRVIQAWCTVNACKGVERIPTPKPPVDCKQVVRILADAKLSSFSQQN 120

Query: 121 CLRRLRSIASASETNKRCMEAAGVVEFLVGVVCNSINVVDSSFEDYAF--DDQLANLGDE 180
           CLRRL+SIA+ASE+NKRCMEAAG VEFL G+VCNS N V+ S EDYA+  DDQLANLG E
Sbjct: 121 CLRRLQSIAAASESNKRCMEAAGAVEFLAGIVCNSTN-VEFSLEDYAYLIDDQLANLGFE 180

Query: 181 ALSILYKLQISESGLKSLLANNGGVFLTTLTKILQNGTYGSRAYAVMLLQSMFEVADQIQ 240
           ALSILYKLQISESGLK LLANNGG+F++TLTKILQNGTYGSR Y+VMLL SMFEVADQIQ
Sbjct: 181 ALSILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRTYSVMLLTSMFEVADQIQ 240

Query: 241 ILSLNADFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAVPVLIDLLLD 300
           ILSLN DFF+EIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAV+AGAVPVLIDLLLD
Sbjct: 241 ILSLNTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLD 300

Query: 301 LDSSEKRLCEMILVALDLLCGCADGRAELLNHAAGMAVVSKKILRVSSLGSEKAVGILCS 360
           LDSSEKR CEMILV LDLLCGCADGRAE+LNHAAG+A+VSKKILRVS+LGSEKAVGILCS
Sbjct: 301 LDSSEKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCS 360

Query: 361 VARYSASQTVVQEMVRLGVVTKLCFLLQVVGVGAKAKEKAREILKLHGRTWRNSSCLPSS 420
           +AR+SAS++VVQEM+RLGVVTKLCFLLQV G G KAKEKA+EILKLHGRTWRNSSCLPSS
Sbjct: 361 IARFSASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSS 420

Query: 421 LRSSYP 423
           LRSS+P
Sbjct: 421 LRSSFP 422

[Close]

BLAST of Spg013218 vs. NCBI nr
Match: KAG7033684.1 (E3 ubiquitin-protein ligase PUB22, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 696.8 bits (1797), Expect = 1.1e-196
Identity = 365/426 (85.68%), Postives = 396/426 (92.96%), Query Frame = 0


Query: 1   MAEADQPVDQVDPPPFFLCPISLQIMKDPVTVASGMTYDRENIERWLFSDKHKTCPVTQV 60
           MAE DQ   ++DPPPFFLCPISLQIMKDPVTVASG+TYDR++IE+WLFSD HKTCPVTQV
Sbjct: 1   MAELDQ---ELDPPPFFLCPISLQIMKDPVTVASGITYDRKSIEKWLFSDGHKTCPVTQV 60

Query: 61  VLSDFGLTPNHTLRRVIQAWCTVNG--GIERIPTPKAPVDRKQVVEILAEAKLSPFSQQN 120
           VLS F +TPN TLRRVIQAWCTVN   GIERIPTPK PVD KQVV ILA+AKLSPFSQQN
Sbjct: 61  VLSSFDITPNITLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQN 120

Query: 121 CLRRLRSIASASETNKRCMEAAGVVEFLVGVVCNSINVVDSSFEDYAF--DDQLANLGDE 180
           CLRRLRSIA+ASE+NKRCMEAAG VEFL G+VCNS N V+ S EDYA+  DDQLANLGDE
Sbjct: 121 CLRRLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTN-VEFSLEDYAYLIDDQLANLGDE 180

Query: 181 ALSILYKLQISESGLKSLLANNGGVFLTTLTKILQNGTYGSRAYAVMLLQSMFEVADQIQ 240
           ALSIL+KLQISESGLK LLANNGG+F++TLTKILQNGTYGSRAY+VMLL SMFEVADQIQ
Sbjct: 181 ALSILHKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQ 240

Query: 241 ILSLNADFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAVPVLIDLLLD 300
           ILSLN DFF+EIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAV+AGAVPVLIDLLLD
Sbjct: 241 ILSLNTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLD 300

Query: 301 LDSSEKRLCEMILVALDLLCGCADGRAELLNHAAGMAVVSKKILRVSSLGSEKAVGILCS 360
           LDSSEKR CEMILV LDLLCGCADGRAE+LNHAAG+A+VSKKILRVS+LGSEKAVGILCS
Sbjct: 301 LDSSEKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCS 360

Query: 361 VARYSASQTVVQEMVRLGVVTKLCFLLQVVGVGAKAKEKAREILKLHGRTWRNSSCLPSS 420
           +AR+SAS++VVQEM+RLGVVTKLCFLLQV G G KAKEKA+EILKLHGRTWRNSSCLPSS
Sbjct: 361 IARFSASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSS 420

Query: 421 LRSSYP 423
           LRSS+P
Sbjct: 421 LRSSFP 422

[Close]

BLAST of Spg013218 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 1.4e-117
Identity = 229/432 (53.01%), Postives = 307/432 (71.06%), Query Frame = 0


Query: 10  QVDPPPFFLCPISLQIMKDPVTVASGMTYDRENIERWLFSDKHKTCPVTQVVLSDFGLTP 69
           +++ P FFLCPISL IMKDPV V++G+TYDRE+IE+WLFS K  +CPVT+ V+++  LTP
Sbjct: 4   EIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDLTP 63

Query: 70  NHTLRRVIQAWCTVNG--GIERIPTPKAPVDRKQVVEILAEAKLSPFSQQNCLRRLRSIA 129
           NHTLRR+IQ+WCT+N   GIERIPTPK P+ + ++ +++ E+  S  +Q  CL+RLR I 
Sbjct: 64  NHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQIV 123

Query: 130 SASETNKRCMEAAGVVEFLVGVVCNSINVVD--------SSFEDYAFDDQLAN------- 189
           S + TNKRC+EAA V EFL  +V NS++  +        S+  D    + L N       
Sbjct: 124 SENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSSRS 183

Query: 190 LGDEALSILYKLQISESGLKSLLANNGGVFLT-TLTKILQNGTYGSRAYAVMLLQSMFEV 249
           L DEALS+LY L  SE+ LKSLL N  G  L  TLTKI+Q G Y SRAYA +LL+ + EV
Sbjct: 184 LMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLLEV 243

Query: 250 ADQIQILSLNADFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAVPVLI 309
           AD +QI+ L  + F E++QIL DQIS +A++SA+++L+  C   RNR KAV+ G + ++I
Sbjct: 244 ADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGTISMII 303

Query: 310 DLLL-DLDSSEKRLCEMILVALDLLCGCADGRAELLNHAAGMAVVSKKILRVSSLGSEKA 369
           +LL+ D  SSE+R  EM +V LD+LC CA+GRAE LNH A +AVVSKKILRVS + SE+A
Sbjct: 304 ELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQITSERA 363

Query: 370 VGILCSVARYSASQTVVQEMVRLGVVTKLCFLLQVVGVGAKAKEKAREILKLHGRTWRNS 423
           V +L SV R+ A+ +++QEM++LGVV KLC +LQ V  G K KEKA+E+LKLH R WR S
Sbjct: 364 VRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQ-VSCGNKTKEKAKELLKLHARVWRES 423

[Close]

BLAST of Spg013218 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 1.4e-112
Identity = 212/418 (50.72%), Postives = 302/418 (72.25%), Query Frame = 0


Query: 9   DQVDPPPFFLCPISLQIMKDPVTVASGMTYDRENIERWLFSDKHKTCPVTQVVLSDFGLT 68
           ++++ PPFFLCPISL+IMKDPV V++G+TYDR++IE+WLF+ K  +CPVT+  ++D  LT
Sbjct: 8   EEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLT 67

Query: 69  PNHTLRRVIQAWCTVNG--GIERIPTPKAPVDRKQVVEILAEAKLSPFSQQNCLRRLRSI 128
           PNHTLRR+IQ+WCT+N   G+ERIPTP+ P+ + ++ +++ ++  S  +Q  CL+RLR I
Sbjct: 68  PNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQI 127

Query: 129 ASASETNKRCMEAAGVVEFLVGVVCNSINVVDSSFEDYAFDDQLANLGDEALSILYKLQI 188
            S + TNKRC+EAAGV EFL  +V N              D +  +L DEAL++LY L+ 
Sbjct: 128 VSENATNKRCLEAAGVPEFLANIVSN--------------DSENGSLTDEALNLLYHLET 187

Query: 189 SESGLKSLLAN-NGGVFLTTLTKILQNGTYGSRAYAVMLLQSMFEVADQIQILSLNADFF 248
           SE+ LK+LL N      + +LTKI+Q G Y SR YA +LL+++ EVAD +Q ++L  + F
Sbjct: 188 SETVLKNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKPEVF 247

Query: 249 IEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAVPVLIDLLLDLD-SSEKRL 308
            E+VQIL D+IS++A+K+A+ +L+ +C   RNR KAV+AG + V+I+LL+D   +SE+R 
Sbjct: 248 TEVVQILDDRISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRG 307

Query: 309 CEMILVALDLLCGCADGRAELLNHAAGMAVVSKKILRVSSLGSEKAVGILCSVARYSASQ 368
            EM +V LDLLC CA+GRAE LNH A +AVV KKILRVS   S++AV +L SV R+ A+ 
Sbjct: 308 PEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATP 367

Query: 369 TVVQEMVRLGVVTKLCFLLQVVGVGAKAKEKAREILKLHGRTWRNSSCLPSSLRSSYP 423
            ++ EM++LGVV KLC +LQ V  G K KEKA+E+LKLH R W++S CLP ++  +YP
Sbjct: 368 ALLHEMLQLGVVAKLCLVLQ-VSCGGKTKEKAKELLKLHARVWKDSPCLPKNMILAYP 410

[Close]

BLAST of Spg013218 vs. ExPASy Swiss-Prot
Match: Q9SF15 (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 1.5e-61
Identity = 167/454 (36.78%), Postives = 249/454 (54.85%), Query Frame = 0


Query: 5   DQPVDQVDPPPFFLCPISLQIMKDPVTVASGMTYDRENIERWLFSDKHKTCPVTQVVLS- 64
           DQ  ++++ P +F+CPISL+IMKDPVT  SG+TYDR+NI +WL  +K  +CPVT+  L  
Sbjct: 2   DQEEEEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPL 61

Query: 65  DFGLTPNHTLRRVIQAWCTVN--GGIERIPTPKAPVDRKQVVEILAEAK---LSPFSQQN 124
           D  LTPNH LRR+IQ WC  N   G+ RI TP+ P  +  VVE +   K        ++ 
Sbjct: 62  DSDLTPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREE 121

Query: 125 CLRRLRSIASASETNKRCMEAAGVVEFLVGVVCNSINVVDSSFEDYAFDDQLANLGDEAL 184
            L++L  +A     N+R M   GV + L+      + VV  + ED     ++  L DE+L
Sbjct: 122 TLQKLEVLA-MDGNNRRLMCECGVHKSLI------LFVVKCTSEDEDGRRRIKGL-DESL 181

Query: 185 SILYKLQISESGLKSLLANNGGVFLTTLTKILQNGTYGSRAYAVMLLQSMFEVADQIQIL 244
            +L+ + I  +  K++L  N  V + +LT +L    + S+AY ++LL+++ E      + 
Sbjct: 182 RLLHLIGIPSNDAKTILMENDRV-MESLTWVLHQEDFLSKAYTIVLLRNLTEYTSSHIVE 241

Query: 245 SLNADFFIEIVQILKDQIS-----------------------------------KQASKS 304
            LN + F  I+  LKD ++                                   KQA  +
Sbjct: 242 RLNPEIFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTA 301

Query: 305 ALKLLIGLCSSSRNRVKAVQAGAVPVLIDLLLDLDSSEKRLCEMILVALDLLCGCADGRA 364
           AL +L+   S SRNR   V  GAV  LI+L +   + EKR+ E++L  L  LC CA+GRA
Sbjct: 302 ALMILLETSSWSRNRSLLVDLGAVSELIELEISY-TGEKRITELMLGVLSRLCCCANGRA 361

Query: 365 ELLNHAAGMAVVSKKILRVSSLGSEKAVGILCSVARYSASQTVVQEMVRLGVVTKLCFLL 418
           E+L H  G+AVV+K++LRVS    ++A+ IL +V+++S    VV+EMV +G V KLC +L
Sbjct: 362 EILAHRGGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVL 421

[Close]

BLAST of Spg013218 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 4.5e-47
Identity = 146/425 (34.35%), Postives = 227/425 (53.41%), Query Frame = 0


Query: 6   QPVD-QVDPPPFFLCPISLQIMKDPVTVASGMTYDRENIERWLFSDKHKTCPVTQVVLSD 65
           +P+D  +  P  F CPISL++M+DPVTV +G TYDR +IE W+    + TCPVT+  LSD
Sbjct: 6   EPLDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSD 65

Query: 66  FGLTPNHTLRRVIQAWCTVN--GGIERIPTPKAPVDRKQVVEILAEAK------LSPFSQ 125
           F L PNHTLRR+IQ WC  N   G+ERIPTPK P D   V  +L++A       +S  S+
Sbjct: 66  FTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSR 125

Query: 126 QNCLRRLRSIASASETNKRCMEAAGVVEFLVGVVCNSINVVDSSFEDYAFDDQLANLGDE 185
              LRRLR  A  S+ N+  + A    E L+ ++ +              +   + L  E
Sbjct: 126 AAALRRLRGFARDSDKNRVLIAAHNATEILIKILFS--------------ETTSSELVSE 185

Query: 186 ALSILYKLQISESGLKSLLANNGGVFLTTLTKILQNGTYGSRAYAVMLLQSM---FEVAD 245
           +L++L  L I+E      ++++ G  +  LT++L + +  +R  A  L++ +    + AD
Sbjct: 186 SLALLVMLPITEPNQFVSISSDPG-RVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSAD 245

Query: 246 QIQILSLNADFFIEIVQILKDQI-SKQASKSALKLLIGLCSSSRNRVKAVQAGAVPVLID 305
               +S +   F  ++ +L++ I S++A K  +K L  LCS    R  A+ AGA  +LID
Sbjct: 246 LKGSISNSESVFEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILID 305

Query: 306 -LLLDLDSSEKRLCEMILVALDLLCGCADGRAELLNHAAGMAVVSKKILRVSSLGSEKAV 365
            L  D D  +    E  L  ++LLC   +G A    HA  + ++ K ILRVS   +E A 
Sbjct: 306 RLAADFDRCD---TERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAA 365

Query: 366 GILCSVARYSASQTVVQEMVRLGVVTKLCFLLQVVGVGAKAKEKAREILKLHGRTWRNSS 417
           G L  +A  +A +   +E    GVV +L  ++Q      +AK+KA+++LKL   +W + +
Sbjct: 366 GAL--LALCTAEERWREEAAGAGVVVQLLLMVQ-SECTERAKKKAQKLLKLLRDSWPDYN 409

[Close]

BLAST of Spg013218 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 4.5e-47
Identity = 147/424 (34.67%), Postives = 226/424 (53.30%), Query Frame = 0


Query: 6   QPVD-QVDPPPFFLCPISLQIMKDPVTVASGMTYDRENIERWLFSDKHKTCPVTQVVLSD 65
           +P+D  +  P  F CPISL +M DPVT+++G TYDR +I+ W+ +  + TCPVT+V LSD
Sbjct: 6   EPLDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWI-AMGNTTCPVTRVALSD 65

Query: 66  FGLTPNHTLRRVIQAWCTVN--GGIERIPTPKAPVDRKQVVEILAEAK------LSPFSQ 125
           F L PNHTLRR+IQ WC  N   G+ERIPTPK P D   V  +L++A       +S  S+
Sbjct: 66  FTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSR 125

Query: 126 QNCLRRLRSIASASETNKRCMEAAGVVEFLVGVVCNSINVVDSSFEDYAFDDQLANLGDE 185
              +RRLR +A  SE N+  +      E LV ++   I     S E          L  E
Sbjct: 126 AAAIRRLRGLARDSEKNRVLIAGHNAREILVRILFADIETTSLSSE----------LVSE 185

Query: 186 ALSILYKLQISESGLKSLLANNGGVFLTTLTKILQNGTYGSRAYAVMLLQSMFEVADQIQ 245
           +L++L  L ++E+  +++ ++   V    +T++L + +   R  A  L++ +   A  + 
Sbjct: 186 SLALLVLLHMTETECEAVASDPSRVGF--MTRLLFDSSIEIRVNAAALIEMVLTGAKSMD 245

Query: 246 ---ILSLNADFFIEIVQILKDQI-SKQASKSALKLLIGLCSSSRNRVKAVQAGAVPVLID 305
              I+S +   F  ++ +LK+ I S++A K  +K +  LC   + R  A+ AGA  +LID
Sbjct: 246 LKLIISGSDSIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILID 305

Query: 306 -LLLDLDSSEKRLCEMILVALDLLCGCADGRAELLNHAAGMAVVSKKILRVSSLGSEKAV 365
            L  D D  +    E  L  ++LLC   +G A    HA  + ++ K ILRVS   +E A 
Sbjct: 306 RLAADFDRCD---TERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAA 365

Query: 366 GILCSVARYSASQTVVQEMVRLGVVTKLCFLLQVVGVGAKAKEKAREILKLHGRTWRNSS 416
           G L  +A  +A +    E    G+VT+L  L+Q      +AK KA+ +LKL   +W + S
Sbjct: 366 GAL--LALCTAEERCRDEAAAAGLVTQLLLLVQ-SDCTERAKRKAQMLLKLLRDSWPDDS 410

[Close]

BLAST of Spg013218 vs. ExPASy TrEMBL
Match: A0A6J1EEF1 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111432478 PE=4 SV=1)

HSP 1 Score: 706.4 bits (1822), Expect = 7.0e-200
Identity = 364/413 (88.14%), Postives = 387/413 (93.70%), Query Frame = 0


Query: 10  QVDPPPFFLCPISLQIMKDPVTVASGMTYDRENIERWLFSDKHKTCPVTQVVLSDFGLTP 69
           +VDPPPFFLCPISLQIMKDPVTVASG+TYDRENIERWLFSDKH TCPVTQVVLSDF +TP
Sbjct: 3   EVDPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLFSDKHTTCPVTQVVLSDFDITP 62

Query: 70  NHTLRRVIQAWCTVNGGIERIPTPKAPVDRKQVVEILAEAKLSPFSQQNCLRRLRSIASA 129
           N+TL R+IQAWCTVN  +ERIPTPK PVD+KQVV+ILA+ KLSPFSQQNCLRRL SIASA
Sbjct: 63  NNTLSRLIQAWCTVNNRVERIPTPKPPVDKKQVVKILADVKLSPFSQQNCLRRLESIASA 122

Query: 130 SETNKRCMEAAGVVEFLVGVVCNSINVVDSSFEDYAFDDQLANLGDEALSILYKLQISES 189
           +E NKRCMEAAG VEFLVGVVCNSI  ++SSF D+AFDD LANLGDEALSILYKLQISES
Sbjct: 123 NEMNKRCMEAAGAVEFLVGVVCNSI-TIESSFPDFAFDDPLANLGDEALSILYKLQISES 182

Query: 190 GLKSLLANNGGVFLTTLTKILQNGTYGSRAYAVMLLQSMFEVADQIQILSLNADFFIEIV 249
           GLK LLANNGG+F+TTLTK LQNGTYGSRAYA MLLQSMFEVAD+IQI SLN +FF+EIV
Sbjct: 183 GLKFLLANNGGIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIRSLNLEFFVEIV 242

Query: 250 QILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAVPVLIDLLLDLDSSEKRLCEMIL 309
           QILKDQISKQASKSALKLLIGLCSS RNRV AVQAGAVPVLIDLLLDLDSSEKRLCEMIL
Sbjct: 243 QILKDQISKQASKSALKLLIGLCSSFRNRVNAVQAGAVPVLIDLLLDLDSSEKRLCEMIL 302

Query: 310 VALDLLCGCADGRAELLNHAAGMAVVSKKILRVSSLGSEKAVGILCSVARYSASQTVVQE 369
           VALDLLCGCADGRAELLNHAAG+AVVSKKILRVSSLGSEKAVGILCS+AR+SASQ+VVQE
Sbjct: 303 VALDLLCGCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSVVQE 362

Query: 370 MVRLGVVTKLCFLLQVVGVGAKAKEKAREILKLHGRTWRNSSCLPSSLRSSYP 423
           M RLGVV KLCFLLQVVG GAKAKEKA+EILKLHGRTWRNSSC PSSLR SYP
Sbjct: 363 MARLGVVVKLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPSSLRPSYP 414

[Close]

BLAST of Spg013218 vs. ExPASy TrEMBL
Match: A0A6J1IKG7 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111478249 PE=4 SV=1)

HSP 1 Score: 704.5 bits (1817), Expect = 2.6e-199
Identity = 367/426 (86.15%), Postives = 397/426 (93.19%), Query Frame = 0


Query: 1   MAEADQPVDQVDPPPFFLCPISLQIMKDPVTVASGMTYDRENIERWLFSDKHKTCPVTQV 60
           MAE DQ   ++DPPPFFLCPISLQIMKDPVTVASG+TYDRE+IERWLFSD+HKTCPVTQV
Sbjct: 1   MAELDQ---ELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQV 60

Query: 61  VLSDFGLTPNHTLRRVIQAWCTVNG--GIERIPTPKAPVDRKQVVEILAEAKLSPFSQQN 120
           VLS F +TPNHT+RRVIQAWCT+N   GIERIPTPK P+D KQVV ILA+AKLSPFSQQN
Sbjct: 61  VLSSFDITPNHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAKLSPFSQQN 120

Query: 121 CLRRLRSIASASETNKRCMEAAGVVEFLVGVVCNSINVVDSSFEDYAF--DDQLANLGDE 180
           CLRRLRSIA+ASE+NKRCMEAAG VEFL G+VCNS N V+ S EDYA+  DDQ ANLGDE
Sbjct: 121 CLRRLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTN-VEFSLEDYAYLIDDQFANLGDE 180

Query: 181 ALSILYKLQISESGLKSLLANNGGVFLTTLTKILQNGTYGSRAYAVMLLQSMFEVADQIQ 240
           ALSILYKLQISESGLK LLANNGG+F++TLTKILQNGTYGSRAY+VMLL SMFEVADQIQ
Sbjct: 181 ALSILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQ 240

Query: 241 ILSLNADFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAVPVLIDLLLD 300
           ILSLN DFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGA+PVLIDLLLD
Sbjct: 241 ILSLNTDFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLIDLLLD 300

Query: 301 LDSSEKRLCEMILVALDLLCGCADGRAELLNHAAGMAVVSKKILRVSSLGSEKAVGILCS 360
           LDSSEKR CEMILV LDLLC CADGRAE+LNHAAG+AVVSKKILRVS+LGSEKAVGILCS
Sbjct: 301 LDSSEKRSCEMILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCS 360

Query: 361 VARYSASQTVVQEMVRLGVVTKLCFLLQVVGVGAKAKEKAREILKLHGRTWRNSSCLPSS 420
           +AR+SAS++VVQEM+RLGVVTKLCFLLQVVG G KAKEKA+EILKLHGRTWRNSSCLPSS
Sbjct: 361 IARFSASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSS 420

Query: 421 LRSSYP 423
           LRSS+P
Sbjct: 421 LRSSFP 422

[Close]

BLAST of Spg013218 vs. ExPASy TrEMBL
Match: A0A6J1GGQ8 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111453790 PE=4 SV=1)

HSP 1 Score: 697.2 bits (1798), Expect = 4.2e-197
Identity = 363/426 (85.21%), Postives = 396/426 (92.96%), Query Frame = 0


Query: 1   MAEADQPVDQVDPPPFFLCPISLQIMKDPVTVASGMTYDRENIERWLFSDKHKTCPVTQV 60
           MAE DQ   ++DPPPFFLCPISLQIMKDPVTVASG+TYDRE+IERWLFSD+HKTCP+TQV
Sbjct: 1   MAELDQ---ELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPITQV 60

Query: 61  VLSDFGLTPNHTLRRVIQAWCTVNG--GIERIPTPKAPVDRKQVVEILAEAKLSPFSQQN 120
           VLS+F +TPNHTLRRVIQAWCTVN   G+ERIPTPK PVD KQVV ILA+AKLS FSQQN
Sbjct: 61  VLSNFDITPNHTLRRVIQAWCTVNACKGVERIPTPKPPVDCKQVVRILADAKLSSFSQQN 120

Query: 121 CLRRLRSIASASETNKRCMEAAGVVEFLVGVVCNSINVVDSSFEDYAF--DDQLANLGDE 180
           CLRRL+SIA+ASE+NKRCMEAAG VEFL G+VCNS N V+ S EDYA+  DDQLANLG E
Sbjct: 121 CLRRLQSIAAASESNKRCMEAAGAVEFLAGIVCNSTN-VEFSLEDYAYLIDDQLANLGFE 180

Query: 181 ALSILYKLQISESGLKSLLANNGGVFLTTLTKILQNGTYGSRAYAVMLLQSMFEVADQIQ 240
           ALSILYKLQISESGLK LLANNGG+F++TLTKILQNGTYGSR Y+VMLL SMFEVADQIQ
Sbjct: 181 ALSILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRTYSVMLLTSMFEVADQIQ 240

Query: 241 ILSLNADFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAVPVLIDLLLD 300
           ILSLN DFF+EIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAV+AGAVPVLIDLLLD
Sbjct: 241 ILSLNTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLD 300

Query: 301 LDSSEKRLCEMILVALDLLCGCADGRAELLNHAAGMAVVSKKILRVSSLGSEKAVGILCS 360
           LDSSEKR CEMILV LDLLCGCADGRAE+LNHAAG+A+VSKKILRVS+LGSEKAVGILCS
Sbjct: 301 LDSSEKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCS 360

Query: 361 VARYSASQTVVQEMVRLGVVTKLCFLLQVVGVGAKAKEKAREILKLHGRTWRNSSCLPSS 420
           +AR+SAS++VVQEM+RLGVVTKLCFLLQV G G KAKEKA+EILKLHGRTWRNSSCLPSS
Sbjct: 361 IARFSASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSS 420

Query: 421 LRSSYP 423
           LRSS+P
Sbjct: 421 LRSSFP 422

[Close]

BLAST of Spg013218 vs. ExPASy TrEMBL
Match: A0A6J1HP11 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111466021 PE=4 SV=1)

HSP 1 Score: 695.3 bits (1793), Expect = 1.6e-196
Identity = 356/413 (86.20%), Postives = 385/413 (93.22%), Query Frame = 0


Query: 10  QVDPPPFFLCPISLQIMKDPVTVASGMTYDRENIERWLFSDKHKTCPVTQVVLSDFGLTP 69
           +VDPPPFFLCPISLQIMKDPVTVASG+TYDRENIERWLF DKH TCPVTQVVLSDF +TP
Sbjct: 3   EVDPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLFFDKHTTCPVTQVVLSDFDITP 62

Query: 70  NHTLRRVIQAWCTVNGGIERIPTPKAPVDRKQVVEILAEAKLSPFSQQNCLRRLRSIASA 129
           N+TL R+IQAW T+N G++RIPTPK P+D+KQV++ILA  KLSPFSQQNCL+RLRSIAS 
Sbjct: 63  NNTLSRLIQAWSTLNDGVDRIPTPKPPIDKKQVIKILANVKLSPFSQQNCLQRLRSIAST 122

Query: 130 SETNKRCMEAAGVVEFLVGVVCNSINVVDSSFEDYAFDDQLANLGDEALSILYKLQISES 189
           +ETNKRCMEAAG VEFLVGV CNSI  ++S+F+D+AFDD LANLGDEALSILYKLQISES
Sbjct: 123 NETNKRCMEAAGAVEFLVGVACNSI-TIESNFQDFAFDDPLANLGDEALSILYKLQISES 182

Query: 190 GLKSLLANNGGVFLTTLTKILQNGTYGSRAYAVMLLQSMFEVADQIQILSLNADFFIEIV 249
           GLK LLANNGG+F+TTLTK LQNGTYGSRAYA MLLQSMFEVAD+IQI SLN +FF+EIV
Sbjct: 183 GLKFLLANNGGIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIRSLNLEFFVEIV 242

Query: 250 QILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAVPVLIDLLLDLDSSEKRLCEMIL 309
           QILKDQISKQASKSALKLLIGLCSS RNRV AVQAGAVPVLIDLLLDLD SEKRLCEMIL
Sbjct: 243 QILKDQISKQASKSALKLLIGLCSSFRNRVNAVQAGAVPVLIDLLLDLDLSEKRLCEMIL 302

Query: 310 VALDLLCGCADGRAELLNHAAGMAVVSKKILRVSSLGSEKAVGILCSVARYSASQTVVQE 369
           VALDLLC CADGRAELLNHAAG+AVVSKKILRVSSLGSEKAVGILCS+AR+SASQ+VVQE
Sbjct: 303 VALDLLCSCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSVVQE 362

Query: 370 MVRLGVVTKLCFLLQVVGVGAKAKEKAREILKLHGRTWRNSSCLPSSLRSSYP 423
           MVRLGVV KLCFLLQVVG GAKAKEKA+EILKLHGRTWRNSSC PSSLR SYP
Sbjct: 363 MVRLGVVMKLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPSSLRPSYP 414

[Close]

BLAST of Spg013218 vs. ExPASy TrEMBL
Match: A0A5A7UVZ1 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold654G00730 PE=4 SV=1)

HSP 1 Score: 660.6 bits (1703), Expect = 4.4e-186
Identity = 342/425 (80.47%), Postives = 382/425 (89.88%), Query Frame = 0


Query: 1   MAEADQPVDQVDPPPFFLCPISLQIMKDPVTVASGMTYDRENIERWLFSDKHKTCPVTQV 60
           MAE D     VDPPP+FLCPISLQIMKDPVT+ASG+TYDRE+IE+WLFSDKHKTCPV+ +
Sbjct: 1   MAELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHI 60

Query: 61  VLSDFGLTPNHTLRRVIQAWCTVNG--GIERIPTPKAPVDRKQVVEILAEAKLSPFSQQN 120
           VLSDF +TPNHTLRRVIQAWCTVN   G+ERIPTPK PVDR+QVV ILA+AKLSPFSQ+N
Sbjct: 61  VLSDFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKN 120

Query: 121 CLRRLRSIASASETNKRCMEAAGVVEFLVGVVCNSINVVDSSFEDYAFDDQLANLGDEAL 180
           CL RLRSIA+A+E+NKRCME+AGVVEFL GVVCNS   ++   EDYAFDD  AN  DEAL
Sbjct: 121 CLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDDMSANSADEAL 180

Query: 181 SILYKLQISESGLKSLLANNGGVFLTTLTKILQNGTYGSRAYAVMLLQSMFEVADQIQIL 240
            IL+KLQISES LK LL+NNGG+F++TLTKILQN +Y SR+Y+VMLL SMFEVADQIQI 
Sbjct: 181 IILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQ 240

Query: 241 SLNADFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAVPVLIDLLLDLD 300
           +L ADFFIEI+QILKDQISKQASKSALKLLI + SS RNRVKAVQAGAVPVLIDLLLDLD
Sbjct: 241 NLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLD 300

Query: 301 SSE-KRLCEMILVALDLLCGCADGRAELLNHAAGMAVVSKKILRVSSLGSEKAVGILCSV 360
           SSE KRLCEMILV LD LCGCADGRAELLNHAAG+AVVSKKILR+S++GSEKAVGIL S+
Sbjct: 301 SSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWSI 360

Query: 361 ARYSASQTVVQEMVRLGVVTKLCFLLQVVGVGAKAKEKAREILKLHGRTWRNSSCLPSSL 420
           AR+S SQ+VVQEMVR+GVVTKLCFLLQVVG G KAKEKA+EILKLHGRTWRNSSCLPS+L
Sbjct: 361 ARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTL 420

Query: 421 RSSYP 423
           RS+YP
Sbjct: 421 RSAYP 425

[Close]

BLAST of Spg013218 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 424.5 bits (1090), Expect = 1.0e-118
Identity = 229/432 (53.01%), Postives = 307/432 (71.06%), Query Frame = 0


Query: 10  QVDPPPFFLCPISLQIMKDPVTVASGMTYDRENIERWLFSDKHKTCPVTQVVLSDFGLTP 69
           +++ P FFLCPISL IMKDPV V++G+TYDRE+IE+WLFS K  +CPVT+ V+++  LTP
Sbjct: 4   EIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDLTP 63

Query: 70  NHTLRRVIQAWCTVNG--GIERIPTPKAPVDRKQVVEILAEAKLSPFSQQNCLRRLRSIA 129
           NHTLRR+IQ+WCT+N   GIERIPTPK P+ + ++ +++ E+  S  +Q  CL+RLR I 
Sbjct: 64  NHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQIV 123

Query: 130 SASETNKRCMEAAGVVEFLVGVVCNSINVVD--------SSFEDYAFDDQLAN------- 189
           S + TNKRC+EAA V EFL  +V NS++  +        S+  D    + L N       
Sbjct: 124 SENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSSRS 183

Query: 190 LGDEALSILYKLQISESGLKSLLANNGGVFLT-TLTKILQNGTYGSRAYAVMLLQSMFEV 249
           L DEALS+LY L  SE+ LKSLL N  G  L  TLTKI+Q G Y SRAYA +LL+ + EV
Sbjct: 184 LMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLLEV 243

Query: 250 ADQIQILSLNADFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAVPVLI 309
           AD +QI+ L  + F E++QIL DQIS +A++SA+++L+  C   RNR KAV+ G + ++I
Sbjct: 244 ADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGTISMII 303

Query: 310 DLLL-DLDSSEKRLCEMILVALDLLCGCADGRAELLNHAAGMAVVSKKILRVSSLGSEKA 369
           +LL+ D  SSE+R  EM +V LD+LC CA+GRAE LNH A +AVVSKKILRVS + SE+A
Sbjct: 304 ELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQITSERA 363

Query: 370 VGILCSVARYSASQTVVQEMVRLGVVTKLCFLLQVVGVGAKAKEKAREILKLHGRTWRNS 423
           V +L SV R+ A+ +++QEM++LGVV KLC +LQ V  G K KEKA+E+LKLH R WR S
Sbjct: 364 VRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQ-VSCGNKTKEKAKELLKLHARVWRES 423

[Close]

BLAST of Spg013218 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 407.9 bits (1047), Expect = 9.8e-114
Identity = 212/418 (50.72%), Postives = 302/418 (72.25%), Query Frame = 0


Query: 9   DQVDPPPFFLCPISLQIMKDPVTVASGMTYDRENIERWLFSDKHKTCPVTQVVLSDFGLT 68
           ++++ PPFFLCPISL+IMKDPV V++G+TYDR++IE+WLF+ K  +CPVT+  ++D  LT
Sbjct: 8   EEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLT 67

Query: 69  PNHTLRRVIQAWCTVNG--GIERIPTPKAPVDRKQVVEILAEAKLSPFSQQNCLRRLRSI 128
           PNHTLRR+IQ+WCT+N   G+ERIPTP+ P+ + ++ +++ ++  S  +Q  CL+RLR I
Sbjct: 68  PNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQI 127

Query: 129 ASASETNKRCMEAAGVVEFLVGVVCNSINVVDSSFEDYAFDDQLANLGDEALSILYKLQI 188
            S + TNKRC+EAAGV EFL  +V N              D +  +L DEAL++LY L+ 
Sbjct: 128 VSENATNKRCLEAAGVPEFLANIVSN--------------DSENGSLTDEALNLLYHLET 187

Query: 189 SESGLKSLLAN-NGGVFLTTLTKILQNGTYGSRAYAVMLLQSMFEVADQIQILSLNADFF 248
           SE+ LK+LL N      + +LTKI+Q G Y SR YA +LL+++ EVAD +Q ++L  + F
Sbjct: 188 SETVLKNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKPEVF 247

Query: 249 IEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAVPVLIDLLLDLD-SSEKRL 308
            E+VQIL D+IS++A+K+A+ +L+ +C   RNR KAV+AG + V+I+LL+D   +SE+R 
Sbjct: 248 TEVVQILDDRISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRG 307

Query: 309 CEMILVALDLLCGCADGRAELLNHAAGMAVVSKKILRVSSLGSEKAVGILCSVARYSASQ 368
            EM +V LDLLC CA+GRAE LNH A +AVV KKILRVS   S++AV +L SV R+ A+ 
Sbjct: 308 PEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATP 367

Query: 369 TVVQEMVRLGVVTKLCFLLQVVGVGAKAKEKAREILKLHGRTWRNSSCLPSSLRSSYP 423
            ++ EM++LGVV KLC +LQ V  G K KEKA+E+LKLH R W++S CLP ++  +YP
Sbjct: 368 ALLHEMLQLGVVAKLCLVLQ-VSCGGKTKEKAKELLKLHARVWKDSPCLPKNMILAYP 410

[Close]

BLAST of Spg013218 vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 239.2 bits (609), Expect = 6.1e-63
Identity = 167/456 (36.62%), Postives = 250/456 (54.82%), Query Frame = 0


Query: 3   EADQPVDQVDPPPFFLCPISLQIMKDPVTVASGMTYDRENIERWLFSDKHKTCPVTQVVL 62
           + DQ  ++++ P +F+CPISL+IMKDPVT  SG+TYDR+NI +WL  +K  +CPVT+  L
Sbjct: 14  QMDQEEEEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPL 73

Query: 63  S-DFGLTPNHTLRRVIQAWCTVN--GGIERIPTPKAPVDRKQVVEILAEAK---LSPFSQ 122
             D  LTPNH LRR+IQ WC  N   G+ RI TP+ P  +  VVE +   K        +
Sbjct: 74  PLDSDLTPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGR 133

Query: 123 QNCLRRLRSIASASETNKRCMEAAGVVEFLVGVVCNSINVVDSSFEDYAFDDQLANLGDE 182
           +  L++L  +A     N+R M   GV + L+      + VV  + ED     ++  L DE
Sbjct: 134 EETLQKLEVLA-MDGNNRRLMCECGVHKSLI------LFVVKCTSEDEDGRRRIKGL-DE 193

Query: 183 ALSILYKLQISESGLKSLLANNGGVFLTTLTKILQNGTYGSRAYAVMLLQSMFEVADQIQ 242
           +L +L+ + I  +  K++L  N  V + +LT +L    + S+AY ++LL+++ E      
Sbjct: 194 SLRLLHLIGIPSNDAKTILMENDRV-MESLTWVLHQEDFLSKAYTIVLLRNLTEYTSSHI 253

Query: 243 ILSLNADFFIEIVQILKDQIS-----------------------------------KQAS 302
           +  LN + F  I+  LKD ++                                   KQA 
Sbjct: 254 VERLNPEIFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAV 313

Query: 303 KSALKLLIGLCSSSRNRVKAVQAGAVPVLIDLLLDLDSSEKRLCEMILVALDLLCGCADG 362
            +AL +L+   S SRNR   V  GAV  LI+L +   + EKR+ E++L  L  LC CA+G
Sbjct: 314 TAALMILLETSSWSRNRSLLVDLGAVSELIELEISY-TGEKRITELMLGVLSRLCCCANG 373

Query: 363 RAELLNHAAGMAVVSKKILRVSSLGSEKAVGILCSVARYSASQTVVQEMVRLGVVTKLCF 418
           RAE+L H  G+AVV+K++LRVS    ++A+ IL +V+++S    VV+EMV +G V KLC 
Sbjct: 374 RAEILAHRGGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCS 433

[Close]

BLAST of Spg013218 vs. TAIR 10
Match: AT1G49780.1 (plant U-box 26 )

HSP 1 Score: 190.3 bits (482), Expect = 3.2e-48
Identity = 147/424 (34.67%), Postives = 226/424 (53.30%), Query Frame = 0


Query: 6   QPVD-QVDPPPFFLCPISLQIMKDPVTVASGMTYDRENIERWLFSDKHKTCPVTQVVLSD 65
           +P+D  +  P  F CPISL +M DPVT+++G TYDR +I+ W+ +  + TCPVT+V LSD
Sbjct: 6   EPLDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWI-AMGNTTCPVTRVALSD 65

Query: 66  FGLTPNHTLRRVIQAWCTVN--GGIERIPTPKAPVDRKQVVEILAEAK------LSPFSQ 125
           F L PNHTLRR+IQ WC  N   G+ERIPTPK P D   V  +L++A       +S  S+
Sbjct: 66  FTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSR 125

Query: 126 QNCLRRLRSIASASETNKRCMEAAGVVEFLVGVVCNSINVVDSSFEDYAFDDQLANLGDE 185
              +RRLR +A  SE N+  +      E LV ++   I     S E          L  E
Sbjct: 126 AAAIRRLRGLARDSEKNRVLIAGHNAREILVRILFADIETTSLSSE----------LVSE 185

Query: 186 ALSILYKLQISESGLKSLLANNGGVFLTTLTKILQNGTYGSRAYAVMLLQSMFEVADQIQ 245
           +L++L  L ++E+  +++ ++   V    +T++L + +   R  A  L++ +   A  + 
Sbjct: 186 SLALLVLLHMTETECEAVASDPSRVGF--MTRLLFDSSIEIRVNAAALIEMVLTGAKSMD 245

Query: 246 ---ILSLNADFFIEIVQILKDQI-SKQASKSALKLLIGLCSSSRNRVKAVQAGAVPVLID 305
              I+S +   F  ++ +LK+ I S++A K  +K +  LC   + R  A+ AGA  +LID
Sbjct: 246 LKLIISGSDSIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILID 305

Query: 306 -LLLDLDSSEKRLCEMILVALDLLCGCADGRAELLNHAAGMAVVSKKILRVSSLGSEKAV 365
            L  D D  +    E  L  ++LLC   +G A    HA  + ++ K ILRVS   +E A 
Sbjct: 306 RLAADFDRCD---TERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAA 365

Query: 366 GILCSVARYSASQTVVQEMVRLGVVTKLCFLLQVVGVGAKAKEKAREILKLHGRTWRNSS 416
           G L  +A  +A +    E    G+VT+L  L+Q      +AK KA+ +LKL   +W + S
Sbjct: 366 GAL--LALCTAEERCRDEAAAAGLVTQLLLLVQ-SDCTERAKRKAQMLLKLLRDSWPDDS 410

[Close]

BLAST of Spg013218 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 190.3 bits (482), Expect = 3.2e-48
Identity = 146/425 (34.35%), Postives = 227/425 (53.41%), Query Frame = 0


Query: 6   QPVD-QVDPPPFFLCPISLQIMKDPVTVASGMTYDRENIERWLFSDKHKTCPVTQVVLSD 65
           +P+D  +  P  F CPISL++M+DPVTV +G TYDR +IE W+    + TCPVT+  LSD
Sbjct: 6   EPLDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSD 65

Query: 66  FGLTPNHTLRRVIQAWCTVN--GGIERIPTPKAPVDRKQVVEILAEAK------LSPFSQ 125
           F L PNHTLRR+IQ WC  N   G+ERIPTPK P D   V  +L++A       +S  S+
Sbjct: 66  FTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSR 125

Query: 126 QNCLRRLRSIASASETNKRCMEAAGVVEFLVGVVCNSINVVDSSFEDYAFDDQLANLGDE 185
              LRRLR  A  S+ N+  + A    E L+ ++ +              +   + L  E
Sbjct: 126 AAALRRLRGFARDSDKNRVLIAAHNATEILIKILFS--------------ETTSSELVSE 185

Query: 186 ALSILYKLQISESGLKSLLANNGGVFLTTLTKILQNGTYGSRAYAVMLLQSM---FEVAD 245
           +L++L  L I+E      ++++ G  +  LT++L + +  +R  A  L++ +    + AD
Sbjct: 186 SLALLVMLPITEPNQFVSISSDPG-RVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSAD 245

Query: 246 QIQILSLNADFFIEIVQILKDQI-SKQASKSALKLLIGLCSSSRNRVKAVQAGAVPVLID 305
               +S +   F  ++ +L++ I S++A K  +K L  LCS    R  A+ AGA  +LID
Sbjct: 246 LKGSISNSESVFEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILID 305

Query: 306 -LLLDLDSSEKRLCEMILVALDLLCGCADGRAELLNHAAGMAVVSKKILRVSSLGSEKAV 365
            L  D D  +    E  L  ++LLC   +G A    HA  + ++ K ILRVS   +E A 
Sbjct: 306 RLAADFDRCD---TERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAA 365

Query: 366 GILCSVARYSASQTVVQEMVRLGVVTKLCFLLQVVGVGAKAKEKAREILKLHGRTWRNSS 417
           G L  +A  +A +   +E    GVV +L  ++Q      +AK+KA+++LKL   +W + +
Sbjct: 366 GAL--LALCTAEERWREEAAGAGVVVQLLLMVQ-SECTERAKKKAQKLLKLLRDSWPDYN 409

The following BLAST results are available for this feature:

BLAST of Spg013218 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022925148.1	1.4e-199	88.14	E3 ubiquitin-protein ligase PUB22-like [Cucurbita moschata]	[more]
XP_022978192.1	5.5e-199	86.15	E3 ubiquitin-protein ligase PUB22-like [Cucurbita maxima]	[more]
XP_023543358.1	9.3e-199	86.38	E3 ubiquitin-protein ligase PUB22-like [Cucurbita pepo subsp. pepo]	[more]
XP_022950790.1	8.7e-197	85.21	E3 ubiquitin-protein ligase PUB22-like [Cucurbita moschata]	[more]
KAG7033684.1	1.1e-196	85.68	E3 ubiquitin-protein ligase PUB22, partial [Cucurbita argyrosperma subsp. argyro...	[more]

BLAST of Spg013218 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SVC6	1.4e-117	53.01	E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ...	[more]
Q84TG3	1.4e-112	50.72	E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ...	[more]
Q9SF15	1.5e-61	36.78	E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 ...	[more]
Q9LT79	4.5e-47	34.35	U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2...	[more]
Q9FXA4	4.5e-47	34.67	U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2...	[more]

BLAST of Spg013218 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EEF1	7.0e-200	88.14	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111432478...	[more]
A0A6J1IKG7	2.6e-199	86.15	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111478249 P...	[more]
A0A6J1GGQ8	4.2e-197	85.21	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111453790...	[more]
A0A6J1HP11	1.6e-196	86.20	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111466021 P...	[more]
A0A5A7UVZ1	4.4e-186	80.47	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]

BLAST of Spg013218 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G52450.1	1.0e-118	53.01	plant U-box 22	[more]
AT2G35930.1	9.8e-114	50.72	plant U-box 23	[more]
AT3G11840.1	6.1e-63	36.62	plant U-box 24	[more]
AT1G49780.1	3.2e-48	34.67	plant U-box 26	[more]
AT3G19380.1	3.2e-48	34.35	plant U-box 25	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003613	U box domain	SMART	SM00504	Ubox_2	coord: 16..80 e-value: 9.0E-33 score: 124.9
IPR003613	U box domain	PFAM	PF04564	U-box	coord: 14..82 e-value: 3.5E-20 score: 72.0
IPR003613	U box domain	PROSITE	PS51698	U_BOX	coord: 12..87 score: 42.28421
IPR011989	Armadillo-like helical	GENE3D	1.25.10.10		coord: 99..406 e-value: 4.1E-17 score: 64.0
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 1..89 e-value: 1.5E-27 score: 97.4
None	No IPR available	PANTHER	PTHR22849	WDSAM1 PROTEIN	coord: 9..422
None	No IPR available	PANTHER	PTHR22849:SF132	E3 UBIQUITIN-PROTEIN LIGASE PUB23	coord: 9..422
None	No IPR available	CDD	cd16664	RING-Ubox_PUB	coord: 17..59 e-value: 3.19058E-22 score: 86.9038
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 8..85
IPR016024	Armadillo-type fold	SUPERFAMILY	48371	ARM repeat	coord: 108..384

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg013218.1	Spg013218.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0004842	ubiquitin-protein transferase activity