Spg013133 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg013133 vs. NCBI nr
Match: KAG6595017.1 (UPF0481 protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1036.6 bits (2679), Expect = 7.6e-299
Identity = 506/531 (95.29%), Postives = 520/531 (97.93%), Query Frame = 0

Query: 1   MVAVFNKELLSWYLITLKLRETVESGLPRNSNSANSVDSHGRPELQLQEHRQIQSESHHV 60
           MVAVFNKELLSWYLITLKL+ETVESGLPRNSNSANSVDSHG+PELQLQE+RQIQSESHHV
Sbjct: 1   MVAVFNKELLSWYLITLKLKETVESGLPRNSNSANSVDSHGKPELQLQEYRQIQSESHHV 60

Query: 61  IIEDEDQTLEEDLESPESEWVISIKEKLDQAHQDEVESSWAKLCIYKVPHYLKDGDDKAV 120
           I+EDEDQTLEED ESPESEWVISIKEKLDQAHQDEVESSWAKLCIYKVPHYLKDGDDKAV
Sbjct: 61  IVEDEDQTLEEDSESPESEWVISIKEKLDQAHQDEVESSWAKLCIYKVPHYLKDGDDKAV 120

Query: 121 VPQIVSLGPYHHGKRRLRQMERHKWRSLYHILERTKHDINLYLDAMKELEEKARSCYEGP 180
           VPQIVSLGPYHHGKRRLRQMERHKWRSLYHILERTKHDIN+YLDAMKELEE ARSCYEGP
Sbjct: 121 VPQIVSLGPYHHGKRRLRQMERHKWRSLYHILERTKHDINIYLDAMKELEENARSCYEGP 180

Query: 181 FSFSSNEFVEMMVLDGCFVLELFRGAAEGFTKLGYPRNDPIFAMRGSMHSIQRDMIMLEN 240
           FSFSSNEFVEMMVLDGCFVLELFRGAAEGF +LGYPRNDPIFAMRGSMHSIQRDMIMLEN
Sbjct: 181 FSFSSNEFVEMMVLDGCFVLELFRGAAEGFKQLGYPRNDPIFAMRGSMHSIQRDMIMLEN 240

Query: 241 QLPLFVLDRLLGLQLGENYQKALVAELALRFFDPLTPNDEPLTKSSLNKLESSIGNATAF 300
           QLPLFVLDRLLGLQLGENYQK L+AELALRFFDPLTPNDEPLTKS+LNKLESS+ NATAF
Sbjct: 241 QLPLFVLDRLLGLQLGENYQKGLLAELALRFFDPLTPNDEPLTKSNLNKLESSLRNATAF 300

Query: 301 DPLGYQGGLHCLDVFRRSLLRSGPKLAPKVWIKRRSHANRVADKRRQQLIHCVKELKEAG 360
           DPLG Q GLHCLDVFRRSLLRSG KLAPKVWIKRRSHA+RVADKRRQQLIHCVKELKEAG
Sbjct: 301 DPLGNQDGLHCLDVFRRSLLRSGQKLAPKVWIKRRSHAHRVADKRRQQLIHCVKELKEAG 360

Query: 361 IRFQKKKTDRFWDINFNNGVMQIPRLLIHDGTRSLFLNLIAFEQCHLDCSNDITSYVVFM 420
           IRFQKKKTDRFWDINFNNGVMQIPRLLIHDGTRSLFLNLIAFEQCHLDCSNDITSYVVFM
Sbjct: 361 IRFQKKKTDRFWDINFNNGVMQIPRLLIHDGTRSLFLNLIAFEQCHLDCSNDITSYVVFM 420

Query: 421 DNLIDSHEDVAYLHYCGIIEHWLGSDEEVAELFNRLCQEVVYDINDSYLSQLSEDVNRYY 480
           DNLIDSHEDVAYLHYCGIIEHWLGSDEEVAELFNRLCQEVVYDINDSYLSQ+SEDVN YY
Sbjct: 421 DNLIDSHEDVAYLHYCGIIEHWLGSDEEVAELFNRLCQEVVYDINDSYLSQVSEDVNHYY 480

Query: 481 NHKWNAWRATLKHNYFSNPWAIISLVAAVVLLLLTFAQAFYGVYAYYRPPN 532
           NH+WNAWRA+LKHNYFSNPWAIISL+AAVVLLLLTFAQ FYGVY YYRPPN
Sbjct: 481 NHRWNAWRASLKHNYFSNPWAIISLIAAVVLLLLTFAQTFYGVYGYYRPPN 531

BLAST of Spg013133 vs. NCBI nr
Match: XP_023003973.1 (UPF0481 protein At3g47200-like [Cucurbita maxima])

HSP 1 Score: 1033.5 bits (2671), Expect = 6.4e-298
Identity = 504/531 (94.92%), Postives = 519/531 (97.74%), Query Frame = 0

Query: 1   MVAVFNKELLSWYLITLKLRETVESGLPRNSNSANSVDSHGRPELQLQEHRQIQSESHHV 60
           MVAVFNKELLSWYLITLKL+ETVESGLPRNSNS NSVDSHG+P+LQLQE+RQIQSESHHV
Sbjct: 1   MVAVFNKELLSWYLITLKLKETVESGLPRNSNSPNSVDSHGKPDLQLQEYRQIQSESHHV 60

Query: 61  IIEDEDQTLEEDLESPESEWVISIKEKLDQAHQDEVESSWAKLCIYKVPHYLKDGDDKAV 120
           I+EDEDQ LEED ESPESEWVISIKEKLDQAHQDEVESSWAKLCIYKVPHYLKDGDDKAV
Sbjct: 61  IVEDEDQKLEEDSESPESEWVISIKEKLDQAHQDEVESSWAKLCIYKVPHYLKDGDDKAV 120

Query: 121 VPQIVSLGPYHHGKRRLRQMERHKWRSLYHILERTKHDINLYLDAMKELEEKARSCYEGP 180
           VPQIVSLGPYHHGKRRLRQMERHKWRSLYHILERTKHDIN+YLDAMKELEE ARSCYEGP
Sbjct: 121 VPQIVSLGPYHHGKRRLRQMERHKWRSLYHILERTKHDINIYLDAMKELEENARSCYEGP 180

Query: 181 FSFSSNEFVEMMVLDGCFVLELFRGAAEGFTKLGYPRNDPIFAMRGSMHSIQRDMIMLEN 240
           FSFSSNEFVEMMVLDGCFVLELFRGAAEGF +LGYPRNDPIFAMRGSMHSIQRDMIMLEN
Sbjct: 181 FSFSSNEFVEMMVLDGCFVLELFRGAAEGFKQLGYPRNDPIFAMRGSMHSIQRDMIMLEN 240

Query: 241 QLPLFVLDRLLGLQLGENYQKALVAELALRFFDPLTPNDEPLTKSSLNKLESSIGNATAF 300
           QLPLFVLDRLLG+QLGENYQK L+AELALRFFDPLTPNDEPLTKS+LNKLESS+ NATAF
Sbjct: 241 QLPLFVLDRLLGIQLGENYQKGLLAELALRFFDPLTPNDEPLTKSNLNKLESSLRNATAF 300

Query: 301 DPLGYQGGLHCLDVFRRSLLRSGPKLAPKVWIKRRSHANRVADKRRQQLIHCVKELKEAG 360
           DPLG Q GLHCLDVFRRSLLRSG KLAPKVWIKRRSHA+RVADKRRQQLIHCVKELKEAG
Sbjct: 301 DPLGNQDGLHCLDVFRRSLLRSGQKLAPKVWIKRRSHAHRVADKRRQQLIHCVKELKEAG 360

Query: 361 IRFQKKKTDRFWDINFNNGVMQIPRLLIHDGTRSLFLNLIAFEQCHLDCSNDITSYVVFM 420
           IRFQKKKTDRFWDINFNNGVMQIPRLLIHDGTRSLFLNLIAFEQCHLDCSNDITSYVVFM
Sbjct: 361 IRFQKKKTDRFWDINFNNGVMQIPRLLIHDGTRSLFLNLIAFEQCHLDCSNDITSYVVFM 420

Query: 421 DNLIDSHEDVAYLHYCGIIEHWLGSDEEVAELFNRLCQEVVYDINDSYLSQLSEDVNRYY 480
           DNLIDSHEDVAYLHYCGIIEHWLGSDEEVAELFNRLCQEVVYDINDSYLSQLSEDVNRYY
Sbjct: 421 DNLIDSHEDVAYLHYCGIIEHWLGSDEEVAELFNRLCQEVVYDINDSYLSQLSEDVNRYY 480

Query: 481 NHKWNAWRATLKHNYFSNPWAIISLVAAVVLLLLTFAQAFYGVYAYYRPPN 532
           NH+WNAWRA+LKHNYFSNPWAIISL+AAVVLLLLTFAQ FYGVY YYRPPN
Sbjct: 481 NHRWNAWRASLKHNYFSNPWAIISLIAAVVLLLLTFAQTFYGVYGYYRPPN 531

BLAST of Spg013133 vs. NCBI nr
Match: XP_023518140.1 (UPF0481 protein At3g47200-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1032.7 bits (2669), Expect = 1.1e-297
Identity = 504/531 (94.92%), Postives = 518/531 (97.55%), Query Frame = 0

Query: 1   MVAVFNKELLSWYLITLKLRETVESGLPRNSNSANSVDSHGRPELQLQEHRQIQSESHHV 60
           MVAVFNKELLSWYLITLKL+ETVESGLPRNSNSANSVDSHG+PELQLQE+RQIQSESHHV
Sbjct: 1   MVAVFNKELLSWYLITLKLKETVESGLPRNSNSANSVDSHGKPELQLQEYRQIQSESHHV 60

Query: 61  IIEDEDQTLEEDLESPESEWVISIKEKLDQAHQDEVESSWAKLCIYKVPHYLKDGDDKAV 120
           I+EDEDQ LEED ESPESEWVISIKEKLDQAHQDEVESSWAKLCIYKVPHYLKDGDDKAV
Sbjct: 61  IVEDEDQKLEEDSESPESEWVISIKEKLDQAHQDEVESSWAKLCIYKVPHYLKDGDDKAV 120

Query: 121 VPQIVSLGPYHHGKRRLRQMERHKWRSLYHILERTKHDINLYLDAMKELEEKARSCYEGP 180
           VPQIVSLGPYHHGKRRLRQMERHKWRSLYHILERTKHDIN+YLDAMKELEE ARSCYEGP
Sbjct: 121 VPQIVSLGPYHHGKRRLRQMERHKWRSLYHILERTKHDINIYLDAMKELEENARSCYEGP 180

Query: 181 FSFSSNEFVEMMVLDGCFVLELFRGAAEGFTKLGYPRNDPIFAMRGSMHSIQRDMIMLEN 240
           FSFSSNEFVEMMVLDGCFVLELFRGAAEGF +LGYPRNDPIFAMRGSMHSIQRDMIMLEN
Sbjct: 181 FSFSSNEFVEMMVLDGCFVLELFRGAAEGFKQLGYPRNDPIFAMRGSMHSIQRDMIMLEN 240

Query: 241 QLPLFVLDRLLGLQLGENYQKALVAELALRFFDPLTPNDEPLTKSSLNKLESSIGNATAF 300
           QLPLFVLDRLLGLQLGENYQK L+AELALRFFDPLTPNDEPLTKS+LNKLESS+ NATAF
Sbjct: 241 QLPLFVLDRLLGLQLGENYQKGLLAELALRFFDPLTPNDEPLTKSNLNKLESSLRNATAF 300

Query: 301 DPLGYQGGLHCLDVFRRSLLRSGPKLAPKVWIKRRSHANRVADKRRQQLIHCVKELKEAG 360
           DPLG Q GLHCLDVFRRSLLRSG KLAPKVWIKRRSH +RVADKRRQQLIHCVKELKEAG
Sbjct: 301 DPLGNQDGLHCLDVFRRSLLRSGQKLAPKVWIKRRSHTHRVADKRRQQLIHCVKELKEAG 360

Query: 361 IRFQKKKTDRFWDINFNNGVMQIPRLLIHDGTRSLFLNLIAFEQCHLDCSNDITSYVVFM 420
           IRFQKKKTDRFWDINFNNGVMQIPRLLIHDGTRSLFLNLIAFEQCHLDCSNDITSYVVFM
Sbjct: 361 IRFQKKKTDRFWDINFNNGVMQIPRLLIHDGTRSLFLNLIAFEQCHLDCSNDITSYVVFM 420

Query: 421 DNLIDSHEDVAYLHYCGIIEHWLGSDEEVAELFNRLCQEVVYDINDSYLSQLSEDVNRYY 480
           DNLIDSHEDVAYLHYCGIIEHWLGSDEEVAELFNRLCQEVVYDINDSYLSQ+SEDVN YY
Sbjct: 421 DNLIDSHEDVAYLHYCGIIEHWLGSDEEVAELFNRLCQEVVYDINDSYLSQVSEDVNHYY 480

Query: 481 NHKWNAWRATLKHNYFSNPWAIISLVAAVVLLLLTFAQAFYGVYAYYRPPN 532
           NH+WNAWRA+LKHNYFSNPWAIISL+AAVVLLLLTFAQ FYGVY YYRPPN
Sbjct: 481 NHRWNAWRASLKHNYFSNPWAIISLIAAVVLLLLTFAQTFYGVYGYYRPPN 531

BLAST of Spg013133 vs. NCBI nr
Match: XP_022963073.1 (UPF0481 protein At3g47200-like [Cucurbita moschata])

HSP 1 Score: 1031.9 bits (2667), Expect = 1.9e-297
Identity = 504/531 (94.92%), Postives = 518/531 (97.55%), Query Frame = 0

Query: 1   MVAVFNKELLSWYLITLKLRETVESGLPRNSNSANSVDSHGRPELQLQEHRQIQSESHHV 60
           MVAVFNKELLSWYLITLKL+ETVESGLPRNSNSANSVDSHG+PELQLQE+RQIQSESHHV
Sbjct: 1   MVAVFNKELLSWYLITLKLKETVESGLPRNSNSANSVDSHGKPELQLQEYRQIQSESHHV 60

Query: 61  IIEDEDQTLEEDLESPESEWVISIKEKLDQAHQDEVESSWAKLCIYKVPHYLKDGDDKAV 120
           I+EDEDQ LEED ESPESEWVISIKE LDQAHQDEVESSWAKLCIYKVPHYLKDGDDKAV
Sbjct: 61  IVEDEDQKLEEDSESPESEWVISIKEMLDQAHQDEVESSWAKLCIYKVPHYLKDGDDKAV 120

Query: 121 VPQIVSLGPYHHGKRRLRQMERHKWRSLYHILERTKHDINLYLDAMKELEEKARSCYEGP 180
           VPQIVSLGPYHHGKRRLRQMERHKWRSLYHILERTKHDIN+YLDAMKELEE ARSCYEGP
Sbjct: 121 VPQIVSLGPYHHGKRRLRQMERHKWRSLYHILERTKHDINIYLDAMKELEENARSCYEGP 180

Query: 181 FSFSSNEFVEMMVLDGCFVLELFRGAAEGFTKLGYPRNDPIFAMRGSMHSIQRDMIMLEN 240
           FSFSSNEFVEMMVLDGCFVLELFRGAAEGF +LGYPRNDPIFAMRGSMHSIQRDMIMLEN
Sbjct: 181 FSFSSNEFVEMMVLDGCFVLELFRGAAEGFKQLGYPRNDPIFAMRGSMHSIQRDMIMLEN 240

Query: 241 QLPLFVLDRLLGLQLGENYQKALVAELALRFFDPLTPNDEPLTKSSLNKLESSIGNATAF 300
           QLPLFVLDRLLGLQLGENYQK L+AELALRFFDPLTPNDEPLTKS+LNKLESS+ NATAF
Sbjct: 241 QLPLFVLDRLLGLQLGENYQKGLLAELALRFFDPLTPNDEPLTKSNLNKLESSLRNATAF 300

Query: 301 DPLGYQGGLHCLDVFRRSLLRSGPKLAPKVWIKRRSHANRVADKRRQQLIHCVKELKEAG 360
           DPLG Q GLHCLDVFRRSLLRSG KLAPKVWIKRRSHA+RVADKRRQQLIHCVKELKEAG
Sbjct: 301 DPLGNQDGLHCLDVFRRSLLRSGQKLAPKVWIKRRSHAHRVADKRRQQLIHCVKELKEAG 360

Query: 361 IRFQKKKTDRFWDINFNNGVMQIPRLLIHDGTRSLFLNLIAFEQCHLDCSNDITSYVVFM 420
           IRFQKKKTDRFWDINFNNGVMQIPRLLIHDGTRSLFLNLIAFEQCHLDCSNDITSYVVFM
Sbjct: 361 IRFQKKKTDRFWDINFNNGVMQIPRLLIHDGTRSLFLNLIAFEQCHLDCSNDITSYVVFM 420

Query: 421 DNLIDSHEDVAYLHYCGIIEHWLGSDEEVAELFNRLCQEVVYDINDSYLSQLSEDVNRYY 480
           DNLIDSHEDVAYLHYCGIIEHWLGSDEEVAELFNRLCQEVVYDINDSYLSQ+SEDVN YY
Sbjct: 421 DNLIDSHEDVAYLHYCGIIEHWLGSDEEVAELFNRLCQEVVYDINDSYLSQVSEDVNHYY 480

Query: 481 NHKWNAWRATLKHNYFSNPWAIISLVAAVVLLLLTFAQAFYGVYAYYRPPN 532
           NH+WNAWRA+LKHNYFSNPWAIISL+AAVVLLLLTFAQ FYGVY YYRPPN
Sbjct: 481 NHRWNAWRASLKHNYFSNPWAIISLIAAVVLLLLTFAQTFYGVYGYYRPPN 531

BLAST of Spg013133 vs. NCBI nr
Match: XP_038880921.1 (UPF0481 protein At3g47200-like [Benincasa hispida])

HSP 1 Score: 1020.8 bits (2638), Expect = 4.3e-294
Identity = 501/531 (94.35%), Postives = 519/531 (97.74%), Query Frame = 0

Query: 1   MVAVFNKELLSWYLITLKLRETVESGLPRNSNSANSVDSHGRPELQLQEHRQIQSESHHV 60
           MVAVFNKELLSWYLITLKLRETVESGLPRNS SANSVDS G+ E QLQE +QIQSESHHV
Sbjct: 1   MVAVFNKELLSWYLITLKLRETVESGLPRNSLSANSVDSLGKSEPQLQELKQIQSESHHV 60

Query: 61  IIEDEDQTLEEDLESPESEWVISIKEKLDQAHQDEVESSWAKLCIYKVPHYLKDGDDKAV 120
           IIEDEDQ LEED ESPESEWVI+IKEKL+QAHQDEVESSWAKLCIYKVPHYLKDG+DKAV
Sbjct: 61  IIEDEDQKLEEDPESPESEWVITIKEKLNQAHQDEVESSWAKLCIYKVPHYLKDGEDKAV 120

Query: 121 VPQIVSLGPYHHGKRRLRQMERHKWRSLYHILERTKHDINLYLDAMKELEEKARSCYEGP 180
           VPQIVSLGPYHHGKRRLRQMERHKWRSLYHILERTK DI LYLDAMKELEE+AR+CYEGP
Sbjct: 121 VPQIVSLGPYHHGKRRLRQMERHKWRSLYHILERTKQDIKLYLDAMKELEERARNCYEGP 180

Query: 181 FSFSSNEFVEMMVLDGCFVLELFRGAAEGFTKLGYPRNDPIFAMRGSMHSIQRDMIMLEN 240
           FSFSSNEFVEMMVLDGCFVLELFRGAAEGF +LGYPRNDPIFAMRGSMHSIQRDMIMLEN
Sbjct: 181 FSFSSNEFVEMMVLDGCFVLELFRGAAEGFKQLGYPRNDPIFAMRGSMHSIQRDMIMLEN 240

Query: 241 QLPLFVLDRLLGLQLGENYQKALVAELALRFFDPLTPNDEPLTKSSLNKLESSIGNATAF 300
           QLPLFVLDRLLGLQLG++YQK LVAELALRFFDPLTPNDEPLTKSSLNKLESS+GNATAF
Sbjct: 241 QLPLFVLDRLLGLQLGDHYQKGLVAELALRFFDPLTPNDEPLTKSSLNKLESSLGNATAF 300

Query: 301 DPLGYQGGLHCLDVFRRSLLRSGPKLAPKVWIKRRSHANRVADKRRQQLIHCVKELKEAG 360
           DPLGYQ GLHCLDVFRRSLLRSGPKLAPKVW+KRRSHANRVADKRRQQLIHCVKELKEAG
Sbjct: 301 DPLGYQDGLHCLDVFRRSLLRSGPKLAPKVWMKRRSHANRVADKRRQQLIHCVKELKEAG 360

Query: 361 IRFQKKKTDRFWDINFNNGVMQIPRLLIHDGTRSLFLNLIAFEQCHLDCSNDITSYVVFM 420
           +RF+KKKTDRFWDINFNNGVM+IPRLLIHDGTRSLFLNLIAFEQCHLDCSNDITSYVVFM
Sbjct: 361 VRFRKKKTDRFWDINFNNGVMEIPRLLIHDGTRSLFLNLIAFEQCHLDCSNDITSYVVFM 420

Query: 421 DNLIDSHEDVAYLHYCGIIEHWLGSDEEVAELFNRLCQEVVYDINDSYLSQLSEDVNRYY 480
           DNLIDSHEDVAYLHYCGIIEHWLGSDEEVAELFNRLCQEVVYDIN+SYLSQLSEDVNRYY
Sbjct: 421 DNLIDSHEDVAYLHYCGIIEHWLGSDEEVAELFNRLCQEVVYDINNSYLSQLSEDVNRYY 480

Query: 481 NHKWNAWRATLKHNYFSNPWAIISLVAAVVLLLLTFAQAFYGVYAYYRPPN 532
           NH+WNAWRATLKHNYFSNPWAIISLVAAVVLLLLTFAQAFYGVYAYY+PPN
Sbjct: 481 NHRWNAWRATLKHNYFSNPWAIISLVAAVVLLLLTFAQAFYGVYAYYKPPN 531

BLAST of Spg013133 vs. ExPASy Swiss-Prot
Match: Q9SD53 (UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 1.0e-51
Identity = 139/437 (31.81%), Postives = 233/437 (53.32%), Query Frame = 0

Query: 104 CIYKVPHYLKDGDDKAVVPQIVSLGPYHHGKRRLRQMERHKWRSLYHILERTKH---DIN 163
           CI++VP      + KA  P++VS+GPYH+G++ L+ +++HK R L   L+  K    + N
Sbjct: 47  CIFRVPESFVALNPKAYKPKVVSIGPYHYGEKHLQMIQQHKPRLLQLFLDEAKKKDVEEN 106

Query: 164 LYLDAMKELEEKARSCYEGPFSFSSNEFVEMMVLDGCFVLELFRGAAEGFTKLGYPRNDP 223
           + + A+ +LE+K R  Y      + ++ + MMVLDGCF+L +F     G  +L     DP
Sbjct: 107 VLVKAVVDLEDKIRKSYSEELK-TGHDLMFMMVLDGCFILMVFL-IMSGNIELS---EDP 166

Query: 224 IFAMRGSMHSIQRDMIMLENQLPLFVLDRL-LGLQLGENYQKALVAELALRFFDPLTPND 283
           IF++   + SIQ D+++LENQ+P FVL  L +G ++G +     +  +A  FF       
Sbjct: 167 IFSIPWLLSSIQSDLLLLENQVPFFVLQTLYVGSKIGVSSD---LNRIAFHFF------- 226

Query: 284 EPLTKSSLNKLESSIGNATAFDPLGYQGGLHCLDVFRRSLL----RSGPKLAPKVWI--- 343
               K+ ++K        + ++        H LD+ R + L     S    +P V +   
Sbjct: 227 ----KNPIDK------EGSYWEKHRNYKAKHLLDLIRETFLPNTSESDKASSPHVQVQLH 286

Query: 344 KRRSHANRVADKRRQQLIHCVKELKEAGIRF--QKKKTDRFWDINFNNGVMQIPRLLIHD 403
           + +S      D +   LI   K L+  GI+F  ++ K D   ++      +QIP+L    
Sbjct: 287 EGKSGNVPSVDSKAVPLILSAKRLRLQGIKFRLRRSKEDSILNVRLKKNKLQIPQLRFDG 346

Query: 404 GTRSLFLNLIAFEQCHLDCSNDITSYVVFMDNLIDSHEDVAYLHYCG-IIEHWLGSDEEV 463
              S FLN +AFEQ + D SN+IT+Y+VFM  L+++ EDV +L     IIE+  GS+ EV
Sbjct: 347 FISSFFLNCVAFEQFYTDSSNEITTYIVFMGCLLNNEEDVTFLRNDKLIIENHFGSNNEV 406

Query: 464 AELFNRLCQEVVYDINDSYLSQLSEDVNRYYNHKWNAWRATLKHNYFSNPWAIISLVAAV 523
           +E F  + ++VV++++ SYL+ + + VN Y    +N   A  +H +F +PW  +S  A +
Sbjct: 407 SEFFKTISKDVVFEVDTSYLNNVFKGVNEYTKKWYNGLWAGFRHTHFESPWTFLSSCAVL 458

Query: 524 VLLLLTFAQAFYGVYAY 527
            ++LLT  Q+   + +Y
Sbjct: 467 FVILLTMLQSTVAILSY 458

BLAST of Spg013133 vs. ExPASy Swiss-Prot
Match: P0C897 (Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 PE=3 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 2.8e-30
Identity = 131/520 (25.19%), Postives = 219/520 (42.12%), Query Frame = 0

Query: 77  ESEWVISIKEKLDQAHQDEVESSWAKLCIYKVPHYLKDGDDKAVVPQIVSLGPYHHGKRR 136
           E+ WVI++++ LD A  +E +     + I+ VP  L      +  P  VS+GPYH  K  
Sbjct: 18  ETRWVINVQKSLD-AELEEHDLEEVTVSIFNVPKALMCSHPDSYTPHRVSIGPYHCLKPE 77

Query: 137 LRQMERHKWRSLYHILERTK----HDINLYLDAMKELEEKARSCYEGPFSFSSNEFVEMM 196
           L +MER+K      I  +      HD+   ++ ++ +E K R+CY     F+    + +M
Sbjct: 78  LHEMERYKLMIARKIRNQYNSFRFHDL---VEKLQSMEIKIRACYHKYIGFNGETLLWIM 137

Query: 197 VLDGCFVLELFRGAAEGFTKLGYPRNDPIFAMRGSMHSIQRDMIMLENQLPLFVLDRLLG 256
            +D  F++E        F K+   R       R   + I RD++M+ENQ+PLFVL + L 
Sbjct: 138 AVDSSFLIE--------FLKIYSFRKVETLINRVGHNEILRDIMMIENQIPLFVLRKTLE 197

Query: 257 LQLGENYQKA--LVAELALRFFDPLTP-----NDEPLTKSSLNKLESSI----------- 316
            QL E+ + A  L+  +       L+P     +D+ + K+   +    +           
Sbjct: 198 FQL-ESTESADDLLLSVLTGLCKDLSPLVIKFDDDQILKAQFQECNHILDFLYQMIVPRI 257

Query: 317 --------GNATAFDPLGYQGGLHCLDVFRRSLLR---SGP------------------- 376
                         D  G    +  +D  +    R   S P                   
Sbjct: 258 EEEELEEDDEENRADENGGNRAIRFMDEIKHQFKRVFASRPADLILRFPWRIISNLPGFM 317

Query: 377 --KLAPKVWIKR--------RSHANRVADKRRQQLIH-----CVKELKEAGIRFQKKKTD 436
             KL+      R        R  +  + D  +  L+       V +L +AG+RF+     
Sbjct: 318 ALKLSADYLFTRQENEATTTRQESVSILDIEKPPLVEELTIPSVSDLHKAGVRFKPTAHG 377

Query: 437 RFWDINF--NNGVMQIPRLLIHDGTRSLFLNLIAFEQCHLDCSNDITSYVVFMDNLIDSH 496
               + F  N+G   +P + +   T ++  NL+A+E  +       T Y   ++ +IDS 
Sbjct: 378 NISTVTFDSNSGQFYLPVINLDINTETVLRNLVAYEATNTSGPLVFTRYTELINGIIDSE 437

Query: 497 EDVAYLHYCGIIEHWLGSDEEVAELFNRLCQEVVYDINDSYLSQLSEDVNRYYNHKWNAW 528
           EDV  L   G++   L SD+E AE++N + + V       +L +  EDVNRYY  +W   
Sbjct: 438 EDVRLLREQGVLVSRLKSDQEAAEMWNGMSKSVRL-TKVGFLDKTIEDVNRYYTGRWKVK 497

BLAST of Spg013133 vs. ExPASy TrEMBL
Match: A0A6J1KY55 (UPF0481 protein At3g47200-like OS=Cucurbita maxima OX=3661 GN=LOC111497424 PE=4 SV=1)

HSP 1 Score: 1033.5 bits (2671), Expect = 3.1e-298
Identity = 504/531 (94.92%), Postives = 519/531 (97.74%), Query Frame = 0

Query: 1   MVAVFNKELLSWYLITLKLRETVESGLPRNSNSANSVDSHGRPELQLQEHRQIQSESHHV 60
           MVAVFNKELLSWYLITLKL+ETVESGLPRNSNS NSVDSHG+P+LQLQE+RQIQSESHHV
Sbjct: 1   MVAVFNKELLSWYLITLKLKETVESGLPRNSNSPNSVDSHGKPDLQLQEYRQIQSESHHV 60

Query: 61  IIEDEDQTLEEDLESPESEWVISIKEKLDQAHQDEVESSWAKLCIYKVPHYLKDGDDKAV 120
           I+EDEDQ LEED ESPESEWVISIKEKLDQAHQDEVESSWAKLCIYKVPHYLKDGDDKAV
Sbjct: 61  IVEDEDQKLEEDSESPESEWVISIKEKLDQAHQDEVESSWAKLCIYKVPHYLKDGDDKAV 120

Query: 121 VPQIVSLGPYHHGKRRLRQMERHKWRSLYHILERTKHDINLYLDAMKELEEKARSCYEGP 180
           VPQIVSLGPYHHGKRRLRQMERHKWRSLYHILERTKHDIN+YLDAMKELEE ARSCYEGP
Sbjct: 121 VPQIVSLGPYHHGKRRLRQMERHKWRSLYHILERTKHDINIYLDAMKELEENARSCYEGP 180

Query: 181 FSFSSNEFVEMMVLDGCFVLELFRGAAEGFTKLGYPRNDPIFAMRGSMHSIQRDMIMLEN 240
           FSFSSNEFVEMMVLDGCFVLELFRGAAEGF +LGYPRNDPIFAMRGSMHSIQRDMIMLEN
Sbjct: 181 FSFSSNEFVEMMVLDGCFVLELFRGAAEGFKQLGYPRNDPIFAMRGSMHSIQRDMIMLEN 240

Query: 241 QLPLFVLDRLLGLQLGENYQKALVAELALRFFDPLTPNDEPLTKSSLNKLESSIGNATAF 300
           QLPLFVLDRLLG+QLGENYQK L+AELALRFFDPLTPNDEPLTKS+LNKLESS+ NATAF
Sbjct: 241 QLPLFVLDRLLGIQLGENYQKGLLAELALRFFDPLTPNDEPLTKSNLNKLESSLRNATAF 300

Query: 301 DPLGYQGGLHCLDVFRRSLLRSGPKLAPKVWIKRRSHANRVADKRRQQLIHCVKELKEAG 360
           DPLG Q GLHCLDVFRRSLLRSG KLAPKVWIKRRSHA+RVADKRRQQLIHCVKELKEAG
Sbjct: 301 DPLGNQDGLHCLDVFRRSLLRSGQKLAPKVWIKRRSHAHRVADKRRQQLIHCVKELKEAG 360

Query: 361 IRFQKKKTDRFWDINFNNGVMQIPRLLIHDGTRSLFLNLIAFEQCHLDCSNDITSYVVFM 420
           IRFQKKKTDRFWDINFNNGVMQIPRLLIHDGTRSLFLNLIAFEQCHLDCSNDITSYVVFM
Sbjct: 361 IRFQKKKTDRFWDINFNNGVMQIPRLLIHDGTRSLFLNLIAFEQCHLDCSNDITSYVVFM 420

Query: 421 DNLIDSHEDVAYLHYCGIIEHWLGSDEEVAELFNRLCQEVVYDINDSYLSQLSEDVNRYY 480
           DNLIDSHEDVAYLHYCGIIEHWLGSDEEVAELFNRLCQEVVYDINDSYLSQLSEDVNRYY
Sbjct: 421 DNLIDSHEDVAYLHYCGIIEHWLGSDEEVAELFNRLCQEVVYDINDSYLSQLSEDVNRYY 480

Query: 481 NHKWNAWRATLKHNYFSNPWAIISLVAAVVLLLLTFAQAFYGVYAYYRPPN 532
           NH+WNAWRA+LKHNYFSNPWAIISL+AAVVLLLLTFAQ FYGVY YYRPPN
Sbjct: 481 NHRWNAWRASLKHNYFSNPWAIISLIAAVVLLLLTFAQTFYGVYGYYRPPN 531

BLAST of Spg013133 vs. ExPASy TrEMBL
Match: A0A6J1HGP0 (UPF0481 protein At3g47200-like OS=Cucurbita moschata OX=3662 GN=LOC111463383 PE=4 SV=1)

HSP 1 Score: 1031.9 bits (2667), Expect = 9.1e-298
Identity = 504/531 (94.92%), Postives = 518/531 (97.55%), Query Frame = 0

Query: 1   MVAVFNKELLSWYLITLKLRETVESGLPRNSNSANSVDSHGRPELQLQEHRQIQSESHHV 60
           MVAVFNKELLSWYLITLKL+ETVESGLPRNSNSANSVDSHG+PELQLQE+RQIQSESHHV
Sbjct: 1   MVAVFNKELLSWYLITLKLKETVESGLPRNSNSANSVDSHGKPELQLQEYRQIQSESHHV 60

Query: 61  IIEDEDQTLEEDLESPESEWVISIKEKLDQAHQDEVESSWAKLCIYKVPHYLKDGDDKAV 120
           I+EDEDQ LEED ESPESEWVISIKE LDQAHQDEVESSWAKLCIYKVPHYLKDGDDKAV
Sbjct: 61  IVEDEDQKLEEDSESPESEWVISIKEMLDQAHQDEVESSWAKLCIYKVPHYLKDGDDKAV 120

Query: 121 VPQIVSLGPYHHGKRRLRQMERHKWRSLYHILERTKHDINLYLDAMKELEEKARSCYEGP 180
           VPQIVSLGPYHHGKRRLRQMERHKWRSLYHILERTKHDIN+YLDAMKELEE ARSCYEGP
Sbjct: 121 VPQIVSLGPYHHGKRRLRQMERHKWRSLYHILERTKHDINIYLDAMKELEENARSCYEGP 180

Query: 181 FSFSSNEFVEMMVLDGCFVLELFRGAAEGFTKLGYPRNDPIFAMRGSMHSIQRDMIMLEN 240
           FSFSSNEFVEMMVLDGCFVLELFRGAAEGF +LGYPRNDPIFAMRGSMHSIQRDMIMLEN
Sbjct: 181 FSFSSNEFVEMMVLDGCFVLELFRGAAEGFKQLGYPRNDPIFAMRGSMHSIQRDMIMLEN 240

Query: 241 QLPLFVLDRLLGLQLGENYQKALVAELALRFFDPLTPNDEPLTKSSLNKLESSIGNATAF 300
           QLPLFVLDRLLGLQLGENYQK L+AELALRFFDPLTPNDEPLTKS+LNKLESS+ NATAF
Sbjct: 241 QLPLFVLDRLLGLQLGENYQKGLLAELALRFFDPLTPNDEPLTKSNLNKLESSLRNATAF 300

Query: 301 DPLGYQGGLHCLDVFRRSLLRSGPKLAPKVWIKRRSHANRVADKRRQQLIHCVKELKEAG 360
           DPLG Q GLHCLDVFRRSLLRSG KLAPKVWIKRRSHA+RVADKRRQQLIHCVKELKEAG
Sbjct: 301 DPLGNQDGLHCLDVFRRSLLRSGQKLAPKVWIKRRSHAHRVADKRRQQLIHCVKELKEAG 360

Query: 361 IRFQKKKTDRFWDINFNNGVMQIPRLLIHDGTRSLFLNLIAFEQCHLDCSNDITSYVVFM 420
           IRFQKKKTDRFWDINFNNGVMQIPRLLIHDGTRSLFLNLIAFEQCHLDCSNDITSYVVFM
Sbjct: 361 IRFQKKKTDRFWDINFNNGVMQIPRLLIHDGTRSLFLNLIAFEQCHLDCSNDITSYVVFM 420

Query: 421 DNLIDSHEDVAYLHYCGIIEHWLGSDEEVAELFNRLCQEVVYDINDSYLSQLSEDVNRYY 480
           DNLIDSHEDVAYLHYCGIIEHWLGSDEEVAELFNRLCQEVVYDINDSYLSQ+SEDVN YY
Sbjct: 421 DNLIDSHEDVAYLHYCGIIEHWLGSDEEVAELFNRLCQEVVYDINDSYLSQVSEDVNHYY 480

Query: 481 NHKWNAWRATLKHNYFSNPWAIISLVAAVVLLLLTFAQAFYGVYAYYRPPN 532
           NH+WNAWRA+LKHNYFSNPWAIISL+AAVVLLLLTFAQ FYGVY YYRPPN
Sbjct: 481 NHRWNAWRASLKHNYFSNPWAIISLIAAVVLLLLTFAQTFYGVYGYYRPPN 531

BLAST of Spg013133 vs. ExPASy TrEMBL
Match: A0A0A0KID5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G491610 PE=4 SV=1)

HSP 1 Score: 1011.9 bits (2615), Expect = 9.7e-292
Identity = 495/534 (92.70%), Postives = 519/534 (97.19%), Query Frame = 0

Query: 1   MVAVFNKELLSWYLITLKLRETVESGLPRNSNSANSVDSHGRPELQLQEHRQIQSESHHV 60
           MVAVFNKELLSWYLITLKLRETVESGLPRNS SANSVDSHG+ ELQLQE +QIQSESHHV
Sbjct: 1   MVAVFNKELLSWYLITLKLRETVESGLPRNSISANSVDSHGKSELQLQESKQIQSESHHV 60

Query: 61  IIEDEDQTLEE---DLESPESEWVISIKEKLDQAHQDEVESSWAKLCIYKVPHYLKDGDD 120
           I+E+EDQ LEE   +LESP SEWVI+IKEKL+QAHQDEVESSWAKLCIYKVPHYLKDG+D
Sbjct: 61  IVENEDQKLEEEDPELESPVSEWVITIKEKLNQAHQDEVESSWAKLCIYKVPHYLKDGED 120

Query: 121 KAVVPQIVSLGPYHHGKRRLRQMERHKWRSLYHILERTKHDINLYLDAMKELEEKARSCY 180
           KAVVPQIVSLGPYHHGKRRLRQMERHKWRSLYHIL+R+K DI LYLDAMKELEE+AR+CY
Sbjct: 121 KAVVPQIVSLGPYHHGKRRLRQMERHKWRSLYHILDRSKQDIKLYLDAMKELEERARNCY 180

Query: 181 EGPFSFSSNEFVEMMVLDGCFVLELFRGAAEGFTKLGYPRNDPIFAMRGSMHSIQRDMIM 240
           EGPFSFSSNEFVEMMVLDGCFVLELFRGAAEGF +LGYPRNDPIFAMRGSMHSIQRDMIM
Sbjct: 181 EGPFSFSSNEFVEMMVLDGCFVLELFRGAAEGFKQLGYPRNDPIFAMRGSMHSIQRDMIM 240

Query: 241 LENQLPLFVLDRLLGLQLGENYQKALVAELALRFFDPLTPNDEPLTKSSLNKLESSIGNA 300
           LENQLPLFVLDRLL LQLG+NYQK LVAELALRFFDPLTPNDEPLTKSSLNKLESS+GN 
Sbjct: 241 LENQLPLFVLDRLLELQLGDNYQKGLVAELALRFFDPLTPNDEPLTKSSLNKLESSLGNT 300

Query: 301 TAFDPLGYQGGLHCLDVFRRSLLRSGPKLAPKVWIKRRSHANRVADKRRQQLIHCVKELK 360
           TAFDPLGYQ GLHCLDVFRRSLLRSGPKLAPKVW+KRRSHANRVADKRRQQLIHCVKELK
Sbjct: 301 TAFDPLGYQDGLHCLDVFRRSLLRSGPKLAPKVWMKRRSHANRVADKRRQQLIHCVKELK 360

Query: 361 EAGIRFQKKKTDRFWDINFNNGVMQIPRLLIHDGTRSLFLNLIAFEQCHLDCSNDITSYV 420
           +AGIRF+KKKTDRFWDINFNNGVM+IPRLLIHDGTRSLFLNLIAFEQCHLDCSNDITSYV
Sbjct: 361 DAGIRFKKKKTDRFWDINFNNGVMEIPRLLIHDGTRSLFLNLIAFEQCHLDCSNDITSYV 420

Query: 421 VFMDNLIDSHEDVAYLHYCGIIEHWLGSDEEVAELFNRLCQEVVYDINDSYLSQLSEDVN 480
           VFMDNLIDSHEDV+YLHYCGIIEHWLGSDEEVAELFNRLCQEVVYDINDSYLSQLSEDVN
Sbjct: 421 VFMDNLIDSHEDVSYLHYCGIIEHWLGSDEEVAELFNRLCQEVVYDINDSYLSQLSEDVN 480

Query: 481 RYYNHKWNAWRATLKHNYFSNPWAIISLVAAVVLLLLTFAQAFYGVYAYYRPPN 532
           RYYNH+WNAWRATLKHNYFSNPWAIISL+AAVVLLLLTFAQAFYGV+AYY+PPN
Sbjct: 481 RYYNHRWNAWRATLKHNYFSNPWAIISLIAAVVLLLLTFAQAFYGVFAYYKPPN 534

BLAST of Spg013133 vs. ExPASy TrEMBL
Match: A0A5D3CR40 (UPF0481 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G004580 PE=4 SV=1)

HSP 1 Score: 1006.5 bits (2601), Expect = 4.1e-290
Identity = 493/533 (92.50%), Postives = 517/533 (97.00%), Query Frame = 0

Query: 1   MVAVFNKELLSWYLITLKLRETVESGLPRNSNSANSVDSHGRPELQLQEHRQIQSESHHV 60
           MVAVFNKELLSWYLITLKLRETVESGLPR+S SANSVDSHG+ ELQL E +QIQSESH+V
Sbjct: 1   MVAVFNKELLSWYLITLKLRETVESGLPRSSISANSVDSHGKSELQLGEPKQIQSESHNV 60

Query: 61  IIEDEDQTLEED--LESPESEWVISIKEKLDQAHQDEVESSWAKLCIYKVPHYLKDGDDK 120
           IIE+ED  LEED   ESPESEWVI+IKEKL+QAHQDEVESSWAKLCIYKVPHYLKDG+DK
Sbjct: 61  IIENEDHKLEEDPEFESPESEWVITIKEKLNQAHQDEVESSWAKLCIYKVPHYLKDGEDK 120

Query: 121 AVVPQIVSLGPYHHGKRRLRQMERHKWRSLYHILERTKHDINLYLDAMKELEEKARSCYE 180
           AVVPQI+SLGPYHHGKRRLRQMERHKWRSLYHILER+KHDI LYLDAMKELEE+AR+CYE
Sbjct: 121 AVVPQIISLGPYHHGKRRLRQMERHKWRSLYHILERSKHDIKLYLDAMKELEERARNCYE 180

Query: 181 GPFSFSSNEFVEMMVLDGCFVLELFRGAAEGFTKLGYPRNDPIFAMRGSMHSIQRDMIML 240
           GPFSFSSNEFVEMMVLDGCFVLELFRGAAEGF +LGYPRNDPIFAMRGSMHSIQRDMIML
Sbjct: 181 GPFSFSSNEFVEMMVLDGCFVLELFRGAAEGFKQLGYPRNDPIFAMRGSMHSIQRDMIML 240

Query: 241 ENQLPLFVLDRLLGLQLGENYQKALVAELALRFFDPLTPNDEPLTKSSLNKLESSIGNAT 300
           ENQLPLFVLDRLL LQLG+ YQK LVAELALRFFDPLTPNDEPLTKSSLNKLESS+GN T
Sbjct: 241 ENQLPLFVLDRLLELQLGDYYQKGLVAELALRFFDPLTPNDEPLTKSSLNKLESSLGNTT 300

Query: 301 AFDPLGYQGGLHCLDVFRRSLLRSGPKLAPKVWIKRRSHANRVADKRRQQLIHCVKELKE 360
           AFDPLGYQ GLHCLDVFRRSLLRSGPKLAPKVW+KRRSHANRVADKRRQQLIHCVKELK+
Sbjct: 301 AFDPLGYQDGLHCLDVFRRSLLRSGPKLAPKVWMKRRSHANRVADKRRQQLIHCVKELKD 360

Query: 361 AGIRFQKKKTDRFWDINFNNGVMQIPRLLIHDGTRSLFLNLIAFEQCHLDCSNDITSYVV 420
           AGIRF+KKKTDRFWDINFNNGVM+IPRLLIHDGTRSLFLNLIAFEQCHLDCSNDITSYVV
Sbjct: 361 AGIRFKKKKTDRFWDINFNNGVMEIPRLLIHDGTRSLFLNLIAFEQCHLDCSNDITSYVV 420

Query: 421 FMDNLIDSHEDVAYLHYCGIIEHWLGSDEEVAELFNRLCQEVVYDINDSYLSQLSEDVNR 480
           FMDNLIDSHEDVAYLHYCGIIEHWLGSDEEVA+LFNRLCQEVVYDINDSYLSQLSEDVNR
Sbjct: 421 FMDNLIDSHEDVAYLHYCGIIEHWLGSDEEVADLFNRLCQEVVYDINDSYLSQLSEDVNR 480

Query: 481 YYNHKWNAWRATLKHNYFSNPWAIISLVAAVVLLLLTFAQAFYGVYAYYRPPN 532
           YYNH+WNAWRATLKHNYFSNPWAIISL+AAVVLLLLTFAQAFYGV+AYY+PPN
Sbjct: 481 YYNHRWNAWRATLKHNYFSNPWAIISLIAAVVLLLLTFAQAFYGVFAYYKPPN 533

BLAST of Spg013133 vs. ExPASy TrEMBL
Match: A0A1S3B0V1 (UPF0481 protein At3g47200 OS=Cucumis melo OX=3656 GN=LOC103484799 PE=4 SV=1)

HSP 1 Score: 1006.5 bits (2601), Expect = 4.1e-290
Identity = 493/533 (92.50%), Postives = 517/533 (97.00%), Query Frame = 0

Query: 1   MVAVFNKELLSWYLITLKLRETVESGLPRNSNSANSVDSHGRPELQLQEHRQIQSESHHV 60
           MVAVFNKELLSWYLITLKLRETVESGLPR+S SANSVDSHG+ ELQL E +QIQSESH+V
Sbjct: 1   MVAVFNKELLSWYLITLKLRETVESGLPRSSISANSVDSHGKSELQLGEPKQIQSESHNV 60

Query: 61  IIEDEDQTLEED--LESPESEWVISIKEKLDQAHQDEVESSWAKLCIYKVPHYLKDGDDK 120
           IIE+ED  LEED   ESPESEWVI+IKEKL+QAHQDEVESSWAKLCIYKVPHYLKDG+DK
Sbjct: 61  IIENEDHKLEEDPEFESPESEWVITIKEKLNQAHQDEVESSWAKLCIYKVPHYLKDGEDK 120

Query: 121 AVVPQIVSLGPYHHGKRRLRQMERHKWRSLYHILERTKHDINLYLDAMKELEEKARSCYE 180
           AVVPQI+SLGPYHHGKRRLRQMERHKWRSLYHILER+KHDI LYLDAMKELEE+AR+CYE
Sbjct: 121 AVVPQIISLGPYHHGKRRLRQMERHKWRSLYHILERSKHDIKLYLDAMKELEERARNCYE 180

Query: 181 GPFSFSSNEFVEMMVLDGCFVLELFRGAAEGFTKLGYPRNDPIFAMRGSMHSIQRDMIML 240
           GPFSFSSNEFVEMMVLDGCFVLELFRGAAEGF +LGYPRNDPIFAMRGSMHSIQRDMIML
Sbjct: 181 GPFSFSSNEFVEMMVLDGCFVLELFRGAAEGFKQLGYPRNDPIFAMRGSMHSIQRDMIML 240

Query: 241 ENQLPLFVLDRLLGLQLGENYQKALVAELALRFFDPLTPNDEPLTKSSLNKLESSIGNAT 300
           ENQLPLFVLDRLL LQLG+ YQK LVAELALRFFDPLTPNDEPLTKSSLNKLESS+GN T
Sbjct: 241 ENQLPLFVLDRLLELQLGDYYQKGLVAELALRFFDPLTPNDEPLTKSSLNKLESSLGNTT 300

Query: 301 AFDPLGYQGGLHCLDVFRRSLLRSGPKLAPKVWIKRRSHANRVADKRRQQLIHCVKELKE 360
           AFDPLGYQ GLHCLDVFRRSLLRSGPKLAPKVW+KRRSHANRVADKRRQQLIHCVKELK+
Sbjct: 301 AFDPLGYQDGLHCLDVFRRSLLRSGPKLAPKVWMKRRSHANRVADKRRQQLIHCVKELKD 360

Query: 361 AGIRFQKKKTDRFWDINFNNGVMQIPRLLIHDGTRSLFLNLIAFEQCHLDCSNDITSYVV 420
           AGIRF+KKKTDRFWDINFNNGVM+IPRLLIHDGTRSLFLNLIAFEQCHLDCSNDITSYVV
Sbjct: 361 AGIRFKKKKTDRFWDINFNNGVMEIPRLLIHDGTRSLFLNLIAFEQCHLDCSNDITSYVV 420

Query: 421 FMDNLIDSHEDVAYLHYCGIIEHWLGSDEEVAELFNRLCQEVVYDINDSYLSQLSEDVNR 480
           FMDNLIDSHEDVAYLHYCGIIEHWLGSDEEVA+LFNRLCQEVVYDINDSYLSQLSEDVNR
Sbjct: 421 FMDNLIDSHEDVAYLHYCGIIEHWLGSDEEVADLFNRLCQEVVYDINDSYLSQLSEDVNR 480

Query: 481 YYNHKWNAWRATLKHNYFSNPWAIISLVAAVVLLLLTFAQAFYGVYAYYRPPN 532
           YYNH+WNAWRATLKHNYFSNPWAIISL+AAVVLLLLTFAQAFYGV+AYY+PPN
Sbjct: 481 YYNHRWNAWRATLKHNYFSNPWAIISLIAAVVLLLLTFAQAFYGVFAYYKPPN 533

BLAST of Spg013133 vs. TAIR 10
Match: AT3G50120.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 739.6 bits (1908), Expect = 1.8e-213
Identity = 353/531 (66.48%), Postives = 429/531 (80.79%), Query Frame = 0

Query: 1   MVAVFNKELLSWYLITLKLRETVESGLPRNSNSANSVDSHGRPELQLQEHRQIQSESHHV 60
           MVAVF K++LSWYL+TLK+RE +E+    +       +  G PE+   +  Q    +   
Sbjct: 1   MVAVFYKDMLSWYLLTLKIREKLETQNQESVLVNQDQNLPGLPEITRSDQDQNIHNNQQT 60

Query: 61  IIEDEDQTLEEDLESPESEWVISIKEKLDQAHQDEVESSWAKLCIYKVPHYLKDGDDKAV 120
             +     ++E  +    +WVISI +KL+QAH+D+  + W KLCIY+VP+YL++ D+K+ 
Sbjct: 61  QSDPAIYVIKESPKDSRDDWVISITDKLEQAHRDDDTTLWGKLCIYRVPYYLQENDNKSY 120

Query: 121 VPQIVSLGPYHHGKRRLRQMERHKWRSLYHILERTKHDINLYLDAMKELEEKARSCYEGP 180
            PQ VSLGPYHHGK+RLR M+RHKWR++  +L+RT   I +Y+DAM+ELEEKAR+CYEGP
Sbjct: 121 FPQTVSLGPYHHGKKRLRSMDRHKWRAVNRVLKRTNQGIKMYIDAMRELEEKARACYEGP 180

Query: 181 FSFSSNEFVEMMVLDGCFVLELFRGAAEGFTKLGYPRNDPIFAMRGSMHSIQRDMIMLEN 240
            S SSNEF+EM+VLDGCFVLELFRGA EGFT+LGY RNDP+FAMRGSMHSIQRDM+MLEN
Sbjct: 181 LSLSSNEFIEMLVLDGCFVLELFRGAVEGFTELGYARNDPVFAMRGSMHSIQRDMVMLEN 240

Query: 241 QLPLFVLDRLLGLQLGENYQKALVAELALRFFDPLTPNDEPLTKSSLNKLESSIGNATAF 300
           QLPLFVL+RLL LQLG   Q  LVA+LA+RFFDPL P DEPLTKS  +KLE+S+    +F
Sbjct: 241 QLPLFVLNRLLELQLGTRNQTGLVAQLAIRFFDPLMPTDEPLTKSGQSKLENSLARDKSF 300

Query: 301 DPLGYQGGLHCLDVFRRSLLRSGPKLAPKVWIKRRSHANRVADKRRQQLIHCVKELKEAG 360
           DP    G LHCLDVFRRSLLRS PK  P++  KR S   RVADKRRQQLIHCV ELKEAG
Sbjct: 301 DPFADMGELHCLDVFRRSLLRSSPKPEPRLTRKRWSRNTRVADKRRQQLIHCVTELKEAG 360

Query: 361 IRFQKKKTDRFWDINFNNGVMQIPRLLIHDGTRSLFLNLIAFEQCHLDCSNDITSYVVFM 420
           I+F+++KTDRFWD+ F NG ++IPRLLIHDGT+SLFLNLIAFEQCH+D SNDITSY++FM
Sbjct: 361 IKFRRRKTDRFWDMQFKNGYLEIPRLLIHDGTKSLFLNLIAFEQCHIDSSNDITSYIIFM 420

Query: 421 DNLIDSHEDVAYLHYCGIIEHWLGSDEEVAELFNRLCQEVVYDINDSYLSQLSEDVNRYY 480
           DNLIDSHEDV+YLHYCGIIEHWLGSD EVA+LFNRLCQEVV+D  DSYLS+LS +VNRYY
Sbjct: 421 DNLIDSHEDVSYLHYCGIIEHWLGSDSEVADLFNRLCQEVVFDTEDSYLSRLSIEVNRYY 480

Query: 481 NHKWNAWRATLKHNYFSNPWAIISLVAAVVLLLLTFAQAFYGVYAYYRPPN 532
           +HKWNAWRATLKH YF+NPWAI+S  AAV+LL+LTF+Q+FY VYAYY+PP+
Sbjct: 481 DHKWNAWRATLKHKYFNNPWAIVSFCAAVILLVLTFSQSFYAVYAYYKPPS 531

BLAST of Spg013133 vs. TAIR 10
Match: AT3G50170.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 666.0 bits (1717), Expect = 2.5e-191
Identity = 326/533 (61.16%), Postives = 407/533 (76.36%), Query Frame = 0

Query: 4   VFNKELLSWYLITLKLRETVESGLPRNSNSANSVDSHGRPEL-------QLQEHRQIQSE 63
           + NK++L+WYL++LKLR+  ++   ++S      + HG PE+        +Q H+Q  SE
Sbjct: 14  IINKDMLTWYLLSLKLRQKFQT-KNQHSEPVQDPNLHGLPEVTRPNQDQNVQNHKQSHSE 73

Query: 64  SHHVIIEDEDQTLEEDLESPESEWVISIKEKLDQAHQDEVESSWAKLCIYKVPHYLKDGD 123
           S   ++E+  +      E+    WVISI++KL+QA +D+  + W KLCIY+VPHYL++ D
Sbjct: 74  SGKEVVEERPE------ETTGDSWVISIRDKLEQADRDDDTTIWGKLCIYRVPHYLQEND 133

Query: 124 DKAVVPQIVSLGPYHHGKRRLRQMERHKWRSLYHILERTKHDINLYLDAMKELEEKARSC 183
            K+  PQ VSLGPYHHGK+RLR MERHKWR+L  +L+R K  I +Y +AM+ELEEKAR+C
Sbjct: 134 KKSYFPQTVSLGPYHHGKKRLRPMERHKWRALNKVLKRLKQRIEMYTNAMRELEEKARAC 193

Query: 184 YEGPFSFSSNEFVEMMVLDGCFVLELFRGAAEGFTKLGYPRNDPIFAMRGSMHSIQRDMI 243
           YEGP S S NEF EM+VLDGCFVLELFRG  EGFT++GY RNDP+FAMRG MHSIQRDMI
Sbjct: 194 YEGPISLSRNEFTEMLVLDGCFVLELFRGTVEGFTEIGYARNDPVFAMRGLMHSIQRDMI 253

Query: 244 MLENQLPLFVLDRLLGLQLGENYQKALVAELALRFFDPLTPNDEPLTKSSLNKLESSIGN 303
           MLENQLPLFVLDRLL LQLG   Q  +VA +A++FFDPL P  E LTK   +KL + +  
Sbjct: 254 MLENQLPLFVLDRLLELQLGTQNQTGIVAHVAVKFFDPLMPTGEALTKPDQSKLMNWL-- 313

Query: 304 ATAFDPLGYQGGLHCLDVFRRSLLRSGPKLAPKVWIKRRSHANRVADKRRQQLIHCVKEL 363
             + D LG +G LHCLDVFRRSLL+S P    +  +KR +   RV DKR+QQL+HCV EL
Sbjct: 314 EKSLDTLGDKGELHCLDVFRRSLLQSSPTPNTRSLLKRLTRNTRVVDKRQQQLVHCVTEL 373

Query: 364 KEAGIRFQKKKTDRFWDINFNNGVMQIPRLLIHDGTRSLFLNLIAFEQCHLDCSNDITSY 423
           +EAG++F+K+KTDRFWDI F NG ++IP+LLIHDGT+SLF NLIAFEQCH++ SN ITSY
Sbjct: 374 REAGVKFRKRKTDRFWDIEFKNGYLEIPKLLIHDGTKSLFSNLIAFEQCHIESSNHITSY 433

Query: 424 VVFMDNLIDSHEDVAYLHYCGIIEHWLGSDEEVAELFNRLCQEVVYDINDSYLSQLSEDV 483
           ++FMDNLI+S EDV+YLHYCGIIEHWLGSD EVA+LFNRLCQEVV+D  DS+LS+LS DV
Sbjct: 434 IIFMDNLINSSEDVSYLHYCGIIEHWLGSDSEVADLFNRLCQEVVFDPKDSHLSRLSGDV 493

Query: 484 NRYYNHKWNAWRATLKHNYFSNPWAIISLVAAVVLLLLTFAQAFYGVYAYYRP 530
           NRYYN KWN  +ATL H YF+NPWA  S  AAV+LLLLT  Q+FY VYAYY+P
Sbjct: 494 NRYYNRKWNVLKATLTHKYFNNPWAYFSFSAAVILLLLTLCQSFYAVYAYYKP 537

BLAST of Spg013133 vs. TAIR 10
Match: AT3G50130.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 616.7 bits (1589), Expect = 1.8e-176
Identity = 296/470 (62.98%), Postives = 371/470 (78.94%), Query Frame = 0

Query: 67  QTLEEDLESPE---SEWVISIKEKLDQAHQDEVESSWAKLCIYKVPHYLKDGDDKAVVPQ 126
           Q L++  + PE    EWVISI++K++QA +++  +SW KLCIY+VP YL++ + K+  PQ
Sbjct: 100 QKLQKQNQKPEETREEWVISIRDKMEQALREDATTSWDKLCIYRVPQYLQENNKKSYFPQ 159

Query: 127 IVSLGPYHHGKRRLRQMERHKWRSLYHILERTKHDINLYLDAMKELEEKARSCYEGPFSF 186
            VSLGP+HHG + L  M+RHKWR++  ++ RTKHDI +Y+DAMKELE++AR+CYEGP   
Sbjct: 160 TVSLGPFHHGNKHLLPMDRHKWRAVNMVMARTKHDIEMYIDAMKELEDRARACYEGPIDL 219

Query: 187 SSNEFVEMMVLDGCFVLELFRGAAEGFTKLGYPRNDPIFAMRGSMHSIQRDMIMLENQLP 246
           SSN+F EM+VLDGCFVLELFRGA EGF++LGY RNDP+FAMRGSMHSIQRDM+MLENQLP
Sbjct: 220 SSNKFSEMLVLDGCFVLELFRGADEGFSELGYDRNDPVFAMRGSMHSIQRDMVMLENQLP 279

Query: 247 LFVLDRLLGLQLGENYQKALVAELALRFFDPLTPNDEPLTKSSLNKLESSIGNATAFDPL 306
           LFVL+RLL +QLG+ +Q  LV+ LA+RFFDPL P DEPLTK+     + S+     F+P+
Sbjct: 280 LFVLNRLLEIQLGKRHQTGLVSRLAVRFFDPLMPTDEPLTKT-----DDSLEQDKFFNPI 339

Query: 307 G--YQGGLHCLDVFRRSLLRSGPKLAPKVWIKRRSHANRVADKRRQQLIHCVKELKEAGI 366
               +G LHCLDVFRR+LLR      P++   R S   RVADKR+QQLIHCV EL+EAGI
Sbjct: 340 ADKDKGELHCLDVFRRNLLRPCSNPEPRLSRMRWSWRTRVADKRQQQLIHCVTELREAGI 399

Query: 367 RFQKKKTDRFWDINFNNGVMQIPRLLIHDGTRSLFLNLIAFEQCHLDCSNDITSYVVFMD 426
           +F+ +KTDRFWDI F NG ++IP+LLIHDGT+SLF NLIAFEQCH+D SNDITSY++FMD
Sbjct: 400 KFRTRKTDRFWDIRFKNGYLEIPKLLIHDGTKSLFSNLIAFEQCHIDSSNDITSYIIFMD 459

Query: 427 NLIDSHEDVAYLHYCGIIEHWLGSDEEVAELFNRLCQEVVYDINDSYLSQLSEDVNRYYN 486
           NLIDS EDV YLHYCGIIEHWLG+D EVA+LFNRLCQEV +D  +SYLSQLS  V+R Y+
Sbjct: 460 NLIDSSEDVRYLHYCGIIEHWLGNDYEVADLFNRLCQEVAFDPQNSYLSQLSNKVDRNYS 519

Query: 487 HKWNAWRATLKHNYFSNPWAIISLVAAVVLLLLTFAQAFYGVYAYYRPPN 532
            KWN  +A LKH YF+NPWA  S  AA+VLL+LT  Q+F+  Y Y+ PP+
Sbjct: 520 RKWNVLKAILKHKYFNNPWAYFSFFAALVLLVLTLFQSFFTAYPYFNPPS 564

BLAST of Spg013133 vs. TAIR 10
Match: AT3G50140.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 592.4 bits (1526), Expect = 3.5e-169
Identity = 287/468 (61.32%), Postives = 360/468 (76.92%), Query Frame = 0

Query: 67  QTLEEDLESPESEWVISIKEKLDQAHQDEVESSWAKLCIYKVPHYLKDGDDKAVVPQIVS 126
           QT  +  E    EWVI IK+K++Q  +D   +SW K+CIY+VP  LK  D  +  PQ VS
Sbjct: 77  QTRNQQPEETREEWVIWIKDKMEQVMRDAATTSWDKICIYRVPLSLKKSDKNSYFPQAVS 136

Query: 127 LGPYHHGKRRLRQMERHKWRSLYHILERTKHDINLYLDAMKELEEKARSCYEGPFSFSSN 186
           LGPYHHG   LR M+ HKWR++  +++RTK  I +Y+DAMKELEE+AR+CYEGP   SSN
Sbjct: 137 LGPYHHGDEHLRPMDYHKWRAVNMVMKRTKQGIEMYIDAMKELEERARACYEGPIGLSSN 196

Query: 187 EFVEMMVLDGCFVLELFRGAAEGFTKLGYPRNDPIFAMRGSMHSIQRDMIMLENQLPLFV 246
           +F +M+VLDGCFVL+LFRGA EGF+KLGY RNDP+FAMRGSMHSI+RDM+MLENQLPLFV
Sbjct: 197 KFTQMLVLDGCFVLDLFRGAYEGFSKLGYDRNDPVFAMRGSMHSIRRDMLMLENQLPLFV 256

Query: 247 LDRLLGLQLGENYQKALVAELALRFFDPLTPNDEPLTKSSLNKLESS-IGNATAFDPLG- 306
           L+RLL LQLG  YQ  LVA+LA+RFF+PL P     T  S  K+E+S   N   F+P+  
Sbjct: 257 LNRLLELQLGTQYQTGLVAQLAVRFFNPLMP-----TYMSSTKIENSQENNNKFFNPIAD 316

Query: 307 -YQGGLHCLDVFRRSLLRSGPKLAPKVWIKRRSHANRVADKRRQQLIHCVKELKEAGIRF 366
             +  LHCLDVFRRSLL+   K  P++   R S    VADKR+QQL+HCV EL+EAGI+F
Sbjct: 317 KEKEELHCLDVFRRSLLQPSLKPDPRLSRSRWSRKPLVADKRQQQLLHCVTELREAGIKF 376

Query: 367 QKKKTDRFWDINFNNGVMQIPRLLIHDGTRSLFLNLIAFEQCHLDCSNDITSYVVFMDNL 426
           +++K+DRFWDI F NG ++IP+LLIHDGT+SLF NLIA+EQCH+D +NDITSY++FMDNL
Sbjct: 377 KRRKSDRFWDIQFKNGCLEIPKLLIHDGTKSLFSNLIAYEQCHIDSTNDITSYIIFMDNL 436

Query: 427 IDSHEDVAYLHYCGIIEHWLGSDEEVAELFNRLCQEVVYDINDSYLSQLSEDVNRYYNHK 486
           IDS ED+ YLHY  IIEHWLG+D EVA++FNRLCQEV +D+ ++YLS+LS  V+RYYN K
Sbjct: 437 IDSAEDIRYLHYYDIIEHWLGNDSEVADVFNRLCQEVAFDLENTYLSELSNKVDRYYNRK 496

Query: 487 WNAWRATLKHNYFSNPWAIISLVAAVVLLLLTFAQAFYGVYAYYRPPN 532
           WN  +ATLKH YFSNPWA  S  AAV+LLLLT  Q+F+  Y Y++PP+
Sbjct: 497 WNVLKATLKHKYFSNPWAYFSFFAAVILLLLTLFQSFFTSYPYFKPPS 539

BLAST of Spg013133 vs. TAIR 10
Match: AT3G50150.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 572.0 bits (1473), Expect = 5.0e-163
Identity = 284/480 (59.17%), Postives = 358/480 (74.58%), Query Frame = 0

Query: 54  QSESHHVIIEDEDQTLEEDLESP---ESEWVISIKEKLDQAHQDEVESSWAKLCIYKVPH 113
           Q+  +HV    E   +E   E P     EWVISIK+K+++A   +  +SW KLCIY+VP 
Sbjct: 38  QNLHNHVETYVEPSKIEVKEEKPRETREEWVISIKDKMEKALSYDATNSWDKLCIYRVPF 97

Query: 114 YLKDGDDKAVVPQIVSLGPYHHGKRRLRQMERHKWRSLYHILERTKHDINLYLDAMKELE 173
           YL++ D K+ +PQ VS+GPYHHGK  LR MERHKWR++  I+ RTKH+I +Y+DAMKELE
Sbjct: 98  YLQENDKKSYLPQTVSIGPYHHGKVHLRPMERHKWRAVNMIMARTKHNIEMYIDAMKELE 157

Query: 174 EKARSCYEGPFSF-SSNEFVEMMVLDGCFVLELFRGAAEGFTKLGYPRNDPIFAMRGSMH 233
           E+AR+CY+GP    +SNEF EM+VLDGCFVLELF+G  +GF K+GY RNDP+FA RG MH
Sbjct: 158 EEARACYQGPIDMKNSNEFTEMLVLDGCFVLELFKGTIQGFQKIGYARNDPVFAKRGLMH 217

Query: 234 SIQRDMIMLENQLPLFVLDRLLGLQLGENYQKALVAELALRFFDPLTPNDEPLTKSSLNK 293
           SIQRDMIMLENQLPLFVLDRLLGLQ G   Q  +VAE+A+RFF  L P  E LTKS    
Sbjct: 218 SIQRDMIMLENQLPLFVLDRLLGLQTGTPNQTGIVAEVAVRFFKTLMPTSEVLTKS---- 277

Query: 294 LESSIGNATAFDPLGYQGGLHCLDVFRRSLLRSGPKLAPKVWIKRRSHANRVADKRRQQL 353
            E S+ +    D LG  GGLHCLDVF RSL++S      +   +   + +    +++QQL
Sbjct: 278 -ERSLDSQEKSDELGDNGGLHCLDVFHRSLIQSS-----ETTNQGTPYEDMSMVEKQQQL 337

Query: 354 IHCVKELKEAGIRFQKKKTDRFWDINFNNGVMQIPRLLIHDGTRSLFLNLIAFEQCHLDC 413
           IHCV EL+ AG+ F +K+T + WDI F NG ++IP+LLIHDGT+SLF NLIAFEQCH   
Sbjct: 338 IHCVTELRGAGVNFMRKETGQLWDIEFKNGYLKIPKLLIHDGTKSLFSNLIAFEQCHTQS 397

Query: 414 SNDITSYVVFMDNLIDSHEDVAYLHYCGIIEHWLGSDEEVAELFNRLCQEVVYDINDSYL 473
           SN+ITSY++FMDNLI+S +DV+YLH+ GIIEHWLGSD EVA+LFNRLC+EV++D  D YL
Sbjct: 398 SNNITSYIIFMDNLINSSQDVSYLHHDGIIEHWLGSDSEVADLFNRLCKEVIFDPKDGYL 457

Query: 474 SQLSEDVNRYYNHKWNAWRATLKHNYFSNPWAIISLVAAVVLLLLTFAQAFYGVYAYYRP 530
           SQLS +VNRYY+ KWN+ +ATL+  YF+NPWA  S  AAV+LL LTF Q+F+ VYAYY+P
Sbjct: 458 SQLSREVNRYYSRKWNSLKATLRQKYFNNPWAYFSFSAAVILLFLTFFQSFFAVYAYYKP 507