Spg013060 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg013060
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	GDSL esterase/lipase
Location	scaffold1: 11926470 .. 11929543 (-)
RNA-Seq Expression	Spg013060
Synteny	Spg013060

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCGGTTGGCCTACCCGCGGGTCACCACTTCTTCAAAAAGCACTATATATTTTCATGAAAGCCATTCATCTTCAAATCACTTCCACAAATATGGACTCACAAAAGCTACTGTTGTCTCTTTTCACTTTCGCTCTTCTCTCAGGTTCCATTTCTTTGTCCTTGAAACTTTCTTAGTTCGATTTTATCTTTCTGGCATGTGGGGGATTGATAGAAATCAATTATATAAAATCGTCGGCCACAACCAACATTTCAACCCCTTAAATTAACTTTCTCGGGATTTAAATATTATTTTGGTTCATGTGCTTTCAGCTTCCGTTTCTTGTAGTCCTATGTTCAAAATGTTTTTATTTTGGTTTTTGTAATTTCAATTTATATTATTGTCTGTTTCGGTATTTGGTTTCTTCGATCCTTTTCTTTTTCATCTCCTATCCTTTATCAGAGTTTTAGTACACTCAATAAATTTCTTGAAATAAAGACTATAACTTTTTAAAATTATAGAATTTATGTTAGAATTTTTTAATTAGGAACTAAAATAGTTGAATGGTTTCTTTCGAGTACAATAGACTAAAATAAACCAAATTTAAAACCAAAACGAAGAAAATAGACATTTTAAAAGTACTATAAAAATCAAAATAAAAACTCAAAATAGAATAATTAAAATTGTATTCGAACCCTTTTTTAACTCAGACATATGTGTTAACTGAGATGGTGGTAGTTTTTTCTTTGGTTTCAGCAAGGATCAGCCGGTCACCGTCATCTCTGTCGCCAATTCCGGGCAACGAAGCTGTATGTCTTCGGAGACTCGTACGTTGACACCGGTAACATCGGTATATCCAATTTCAGTTCTCGAAAGTTTCCTTATGGCATAACTTTTCCCGGTAAACCCACCGGCCGTTTCTCCGACGGCCGTGTTTTGACCGATTACACAGGTAAACAACTTGTTTCTCTCTCTTCTTAAAAATAAAAAGTCAAAGATAGGGATAGGTCATCAAACAAGTTATCGGTTACGGTTGTTGATTCCGTGTTTTGGTTGGTAATTTGGTATTGAAATTTTAAACTATGTAAGAGGGGTAGTTCAAGTCCTAAATTTTTTTTTTTTTTTACAATATGTGGGGTAGGGGGATTCGAACCACTGATCTCGTGGTCATCAGTACAACTTTATGCTAGTTAAACTAAGCTCTTGGTTGCACTAACTTTTAAGGTTAGATATTAAAATTCCCTCTCCTATAATTTATTTATTTGGGACCCATATGGGAGGACTTTTCTTTTTAATTTTTTTTCCATTTTATTTGGACCTTTTCCCCCTTGAGAGTTTCATACTTCAAATTATATATATACTTTTGGTGATATCCAAAATCACATCTCACCGACCTCATGTCCCACATGGAGATATACAAATATATATTTAAACCCAACAGCTAATTCTTAAAGACTTTGATTTATATTCATAAAAAAAAAAAATTAAAAAAATAAAATAAAAATAAAAAGATTTTGTTTTATAACTATTTCGTTTTTCAGTGTATTGTTTTAAAAATTTATACTTGCATGCTCACAATTTTTTTTTTTCATTTCCCTTTTATAGAAAGACTCTGAACTCTTAAACAAATTTCCAAAGAAATACAAGGGTGGTTTTTTTTTTTTCTTCCTTTCTTTTTAATGAACAGATTGTTAGCTTGGTTTTAAAAAGAAACCCAAAAATAAAATAGTTACCAAATAGAGCCTAATTAATAAGATTTTTCTAATTATGCTGGTCTTTTTCTAGATTTTGTTTAATTTTTTTTGCCATGGTTACCATTGTGCTTAAATATTTTATTACATCAATGTGAGCAATATATTTCCTACTATATATTTTAAAATCTCCCCAAAATCCTCACTAGCCTTTTGATTCTCAACTGACATATATACTTTCATTTTGGTTGAGGTTCCTATTGATTTATGGGACACACATGCGTAAATTTCTCTCCCATTATTATAAATAGTTGTATTTTTTTTTTTTTTTTGTAAATATTTATGTTATACTAACTTTTTTTAAAAAAATATTTACTCTCCCTAAACTCCTATTTTATATTTACACCCTTCAAAATTAGTTAGATTATATTCGATTATAGGGTTGATATGGGTTCAAATGATTTTTAAACTATCGAATTAAAAAGGAAGAAATAAAAAATGATGAAAAGAATGAAATAAAACAAAAAGAGAATGATAGAAAAAAATGTGATAAGAAGATCAAGAAAAAGAAAGTTTGATTCATATGAACAATTGAAAGTTCGTGTAGTGGTTGATTAACCCAATAATATAGAATTATCATTTAGTTTTAAATTATTGGTTCGATATATAGAAGATGTCTTGAATGATTGGAGAAGATTGTCTATTTTAATTATTTTTAAAAAAATAAAATAAAATAGACAGGAGCATGCTGTTGATAATCTTTTTATTTTTTAAAATATGGTGAATATAATCTTTTTAAATTCACAAAGAATGTGTTCTTCTAAAATAATTTAAAATAAAAGTTTAATGTTCAAGATATTTTTCCATTTTATATAGGCATCGGGGTCTTTATCTATTTAAAATAATTATTATCAAATCTTTGCTAATCAAAGTATGATTAAGTTGCCAGCAAGAGCATAGCTCAACTGGCATAAAGTCGTACGGATGACACGAGTTCTGTGGTTCGAATCCTCCACTCCACATATTGTAAAAAAAAAAAAAAGTATGATTAAGTTGACTCATAAAATTGTAACATATTGCAGCAAGATATTTGGGTGTGAAGGCTCCTGTCCCATTTGGAATCTTGAAAAGCTTAGGAATTATTAGAGGGAGAGAAAATGGTGTAAATTTTGCATTTGGAGGGACTGGAGTGTTCAACACATCAGTTTCGTTCCCAAACATGAGGACTCAAATCGACTTATTTGAGAAGTTTATTGTACTCAACAACTCTTGGTTCTTCAAAAAACATGTCTGTTCCTCTCTCGCCCTTGTCTCTGTTTCTGACAATGACTACTCCTTTTACCTTGCTCGAAATGGATCTCTCCAGGTGATCAGTGGAACATTCAGCTCCTTGATCAGTTTTTAA

mRNA sequence

ATGCGGTTGGCCTACCCGCGGGTCACCACTTCTTCAAAAAGCACTATATATTTTCATGAAAGCCATTCATCTTCAAATCACTTCCACAAATATGGACTCACAAAAGCTACTTTTTTTCTTTGGTTTCAGCAAGGATCAGCCGGTCACCGTCATCTCTGTCGCCAATTCCGGGCAACGAAGCTGTATGTCTTCGGAGACTCGTACGTTGACACCGGTAACATCGGTATATCCAATTTCAGTTCTCGAAAGTTTCCTTATGGCATAACTTTTCCCGGTAAACCCACCGGCCGTTTCTCCGACGGCCGTGTTTTGACCGATTACACAGCAAGATATTTGGGTGTGAAGGCTCCTGTCCCATTTGGAATCTTGAAAAGCTTAGGAATTATTAGAGGGAGAGAAAATGGTGTAAATTTTGCATTTGGAGGGACTGGAGTGTTCAACACATCAGTTTCGTTCCCAAACATGAGGACTCAAATCGACTTATTTGAGAAGTTTATTGTACTCAACAACTCTTGGTTCTTCAAAAAACATGTCTGTTCCTCTCTCGCCCTTGTCTCTGTTTCTGACAATGACTACTCCTTTTACCTTGCTCGAAATGGATCTCTCCAGGTGATCAGTGGAACATTCAGCTCCTTGATCAGTTTTTAA

Coding sequence (CDS)

ATGCGGTTGGCCTACCCGCGGGTCACCACTTCTTCAAAAAGCACTATATATTTTCATGAAAGCCATTCATCTTCAAATCACTTCCACAAATATGGACTCACAAAAGCTACTTTTTTTCTTTGGTTTCAGCAAGGATCAGCCGGTCACCGTCATCTCTGTCGCCAATTCCGGGCAACGAAGCTGTATGTCTTCGGAGACTCGTACGTTGACACCGGTAACATCGGTATATCCAATTTCAGTTCTCGAAAGTTTCCTTATGGCATAACTTTTCCCGGTAAACCCACCGGCCGTTTCTCCGACGGCCGTGTTTTGACCGATTACACAGCAAGATATTTGGGTGTGAAGGCTCCTGTCCCATTTGGAATCTTGAAAAGCTTAGGAATTATTAGAGGGAGAGAAAATGGTGTAAATTTTGCATTTGGAGGGACTGGAGTGTTCAACACATCAGTTTCGTTCCCAAACATGAGGACTCAAATCGACTTATTTGAGAAGTTTATTGTACTCAACAACTCTTGGTTCTTCAAAAAACATGTCTGTTCCTCTCTCGCCCTTGTCTCTGTTTCTGACAATGACTACTCCTTTTACCTTGCTCGAAATGGATCTCTCCAGGTGATCAGTGGAACATTCAGCTCCTTGATCAGTTTTTAA

Protein sequence

MRLAYPRVTTSSKSTIYFHESHSSSNHFHKYGLTKATFFLWFQQGSAGHRHLCRQFRATKLYVFGDSYVDTGNIGISNFSSRKFPYGITFPGKPTGRFSDGRVLTDYTARYLGVKAPVPFGILKSLGIIRGRENGVNFAFGGTGVFNTSVSFPNMRTQIDLFEKFIVLNNSWFFKKHVCSSLALVSVSDNDYSFYLARNGSLQVISGTFSSLISF

Homology

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BLAST of Spg013060 vs. NCBI nr
Match: XP_008462556.1 (PREDICTED: GDSL esterase/lipase At5g03610-like [Cucumis melo])

HSP 1 Score: 239.6 bits (610), Expect = 2.5e-59
Identity = 125/181 (69.06%), Postives = 141/181 (77.90%), Query Frame = 0


Query: 37  TFFLWFQQGSAGHRHLCRQFRATKLYVFGDSYVDTGNIGISNFSSRKFPYGITFPGKPTG 96
           T  L  +QGSAGH  L R+FR  KLYVFGDSYVDTGNIGISN+SSRK+PYGITFPGKP+G
Sbjct: 12  TILLLSEQGSAGHNDLIREFRPKKLYVFGDSYVDTGNIGISNYSSRKYPYGITFPGKPSG 71

Query: 97  RFSDGRVLTDYTARYLGVKAPVPFGIL-KSLGIIRGRE---NGVNFAFGGTGVFNTSVSF 156
           RFSDGRVLTDY ARYLG+K P+PF +L K L   +GR+   NGVNFA+GGTGVFNTSVSF
Sbjct: 72  RFSDGRVLTDYVARYLGLKPPIPFRVLFKDLKRRQGRKKNINGVNFAYGGTGVFNTSVSF 131

Query: 157 PNMRTQIDLFEKFIVLNNSWFFKKHVCSSLALVSVSDNDYSFYLARNGSLQVISGTFSSL 214
           PNM TQIDLF KFI        +  + SS+ALVSVS NDYSFYLARNGSLQ   G   S+
Sbjct: 132 PNMTTQIDLFHKFI-------DETDIQSSIALVSVSGNDYSFYLARNGSLQGFKGFMISV 185

[Close]

BLAST of Spg013060 vs. NCBI nr
Match: KAA0025269.1 (GDSL esterase/lipase [Cucumis melo var. makuwa] >TYK07399.1 GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 238.0 bits (606), Expect = 7.4e-59
Identity = 122/166 (73.49%), Postives = 136/166 (81.93%), Query Frame = 0


Query: 43  QQGSAGHRHLCRQFRATKLYVFGDSYVDTGNIGISNFSSRKFPYGITFPGKPTGRFSDGR 102
           +QGSAGH  L R+FR  KLYVFGDSYVDTGNIGISN+SSRK+PYGITFPGKP+GRFSDGR
Sbjct: 19  EQGSAGHNDLIREFRPKKLYVFGDSYVDTGNIGISNYSSRKYPYGITFPGKPSGRFSDGR 78

Query: 103 VLTDYTARYLGVKAPVPFGIL-KSLGIIRGRE---NGVNFAFGGTGVFNTSVSFPNMRTQ 162
           VLTDY ARYLG+K P+PF +L K L   +GR+   NGVNFA+GGTGVFNTSVSFPNM TQ
Sbjct: 79  VLTDYVARYLGLKPPIPFRVLFKDLKRRQGRKKNINGVNFAYGGTGVFNTSVSFPNMTTQ 138

Query: 163 IDLFEKFIVLNNSWFFKKHVCSSLALVSVSDNDYSFYLARNGSLQV 205
           IDLF KFI        +  + SS+ALVSVS NDYSFYLARNGSLQV
Sbjct: 139 IDLFHKFI-------DETDIQSSIALVSVSGNDYSFYLARNGSLQV 177

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BLAST of Spg013060 vs. NCBI nr
Match: KAE8647419.1 (hypothetical protein Csa_004424 [Cucumis sativus])

HSP 1 Score: 236.5 bits (602), Expect = 2.1e-58
Identity = 123/180 (68.33%), Postives = 140/180 (77.78%), Query Frame = 0


Query: 39  FLWFQQGSAGHR-HLCRQFRATKLYVFGDSYVDTGNIGISNFSSRKFPYGITFPGKPTGR 98
           +L  +QGS GH  +L R+F+  KLYVFGDSYVDTGNIGISNFSSRKFPYGITFPGKP+GR
Sbjct: 341 YLVSEQGSGGHNDNLLREFQPKKLYVFGDSYVDTGNIGISNFSSRKFPYGITFPGKPSGR 400

Query: 99  FSDGRVLTDYTARYLGVKAPVPFGILKSLGIIRGRE----NGVNFAFGGTGVFNTSVSFP 158
           FSDGRVLTDY ARYLG+K P+PF + K L   +GR+    NGVNFA+GGTGVFNTSVSFP
Sbjct: 401 FSDGRVLTDYVARYLGLKPPIPFRVFKDLKRRQGRKNKNINGVNFAYGGTGVFNTSVSFP 460

Query: 159 NMRTQIDLFEKFIVLNNSWFFKKHVCSSLALVSVSDNDYSFYLARNGSLQVISGTFSSLI 214
           NM TQIDLF KFI        +  + SS+ALVSVS NDYSFYLARNGSLQ   G   S++
Sbjct: 461 NMTTQIDLFHKFI-------DETDIQSSIALVSVSGNDYSFYLARNGSLQGFKGFMISVV 513

[Close]

BLAST of Spg013060 vs. NCBI nr
Match: XP_031742898.1 (GDSL esterase/lipase At5g03610 [Cucumis sativus])

HSP 1 Score: 231.5 bits (589), Expect = 6.9e-57
Identity = 124/189 (65.61%), Postives = 141/189 (74.60%), Query Frame = 0


Query: 37  TFFLWFQQGSAGHR-HLCRQFRATKLYVFGDSYVDTGNIGISNFSSRKFPYGITFPGKPT 96
           T FL  +QGS GH  +L R+F+  KLYVFGDSYVDTGNIGISNFSSRKFPYGITFPGKP+
Sbjct: 13  TVFLLSEQGSGGHNDNLLREFQPKKLYVFGDSYVDTGNIGISNFSSRKFPYGITFPGKPS 72

Query: 97  GRFSDGRVLTDY-------TARYLGVKAPVPFGILKSLGIIRGRE----NGVNFAFGGTG 156
           GRFSDGRVLTDY        +RYLG+K P+PF + K L   +GR+    NGVNFA+GGTG
Sbjct: 73  GRFSDGRVLTDYVGKNMQLVSRYLGLKPPIPFRVFKDLKRRQGRKNKNINGVNFAYGGTG 132

Query: 157 VFNTSVSFPNMRTQIDLFEKFIVLNNSWFFKKHVCSSLALVSVSDNDYSFYLARNGSLQV 214
           VFNTSVSFPNM TQIDLF KFI        +  + SS+ALVSVS NDYSFYLARNGSLQ 
Sbjct: 133 VFNTSVSFPNMTTQIDLFHKFI-------DETDIQSSIALVSVSGNDYSFYLARNGSLQG 192

[Close]

BLAST of Spg013060 vs. NCBI nr
Match: XP_004143379.2 (GDSL esterase/lipase At5g03610 [Cucumis sativus] >KGN48533.2 hypothetical protein Csa_003217 [Cucumis sativus])

HSP 1 Score: 203.8 bits (517), Expect = 1.5e-48
Identity = 107/185 (57.84%), Postives = 129/185 (69.73%), Query Frame = 0


Query: 37  TFFLWFQQGSAG-------HRHLCRQFRATKLYVFGDSYVDTGNIGISNFSSRKFPYGIT 96
           T  L+  QGSAG       HRH     R TKL+VFGDSYVDTGNI     S+ +FPYGIT
Sbjct: 15  TLSLFSGQGSAGGFYGNLRHRHRSHPSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGIT 74

Query: 97  FPGKPTGRFSDGRVLTDYTARYLGVKAPVPFGILKSLGIIRGRENGVNFAFGGTGVFNTS 156
           FPGKP+GRFSDGRVLTD+ A++LGVK+P+PF I   +G  R +E+G+NFAFGGTGVFNT 
Sbjct: 75  FPGKPSGRFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLKESGINFAFGGTGVFNTL 134

Query: 157 VSFPNMRTQIDLFEKFIVLNNSWFFKKHVCSSLALVSVSDNDYSFYLARNGSLQVISGTF 215
           V  PNM TQIDLFE+     +     + V  SLALVSVS NDYSFYLA NGS Q +    
Sbjct: 135 VPLPNMTTQIDLFEQLRDDESGLISNRDVHLSLALVSVSGNDYSFYLATNGSAQGLKPFI 194

[Close]

BLAST of Spg013060 vs. ExPASy Swiss-Prot
Match: Q9LZS7 (GDSL esterase/lipase At5g03610 OS=Arabidopsis thaliana OX=3702 GN=At5g03610 PE=2 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 3.6e-40
Identity = 84/156 (53.85%), Postives = 110/156 (70.51%), Query Frame = 0


Query: 44  QGSAGHRHLCRQFRATKLYVFGDSYVDTGNIGISNFSSRKFPYGITFPGKPTGRFSDGRV 103
           +GS  + HL   FR TKL+VFGDSY DTGNI  +  SS KFPYGITFPGKP GRFSDGRV
Sbjct: 28  EGSNQNHHL-YPFRPTKLFVFGDSYADTGNIKKAFSSSWKFPYGITFPGKPAGRFSDGRV 87

Query: 104 LTDYTARYLGVKAPVPFGILKSLGIIRGRENGVNFAFGGTGVFNTSVSFPNMRTQIDLFE 163
            TD+ A+++G+K+P+P+   K     +  + G+NFA+GGTGVFNT    PNM TQID+F+
Sbjct: 88  ATDFLAKFVGIKSPIPY-FWKDYAGKKRLQYGMNFAYGGTGVFNTQTPLPNMTTQIDIFQ 147

Query: 164 KFIVLNNSWFFKKHVCSSLALVSVSDNDYSFYLARN 200
             I+     ++   + SS+ALVSV+ NDYS ++A N
Sbjct: 148 N-ILTTGDIYYPPELTSSVALVSVAGNDYSNFIALN 180

[Close]

BLAST of Spg013060 vs. ExPASy Swiss-Prot
Match: Q9SF94 (GDSL esterase/lipase At3g09930 OS=Arabidopsis thaliana OX=3702 GN=At3g09930 PE=2 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 1.0e-34
Identity = 82/166 (49.40%), Postives = 110/166 (66.27%), Query Frame = 0


Query: 36  ATFFLWFQQGSAGHRHLCRQFRATKLYVFGDSYVDTGNIGISNFSSRKFPYGITFPGKPT 95
           ++FFL  +     H +L ++ R  +L+VFGDSY DTGNI  S   S K PYGITFP KP+
Sbjct: 16  SSFFLGAESDYPQHSNL-KRLRPNRLFVFGDSYADTGNIRKSLSDSWKIPYGITFPQKPS 75

Query: 96  GRFSDGRVLTDYTARYLGVKAPVPFGILKSLGIIRGREN---GVNFAFGGTGVFNTSVS- 155
           GRFSDGRV TD+ ARYLG+K+P+P+    +     G+E    G+N+A+GGTGVF T  + 
Sbjct: 76  GRFSDGRVATDFLARYLGIKSPIPY----TWKDYAGKERLLYGMNYAYGGTGVFKTKDNP 135

Query: 156 FPNMRTQIDLFEKFIVLNNSWFFKKHVCSSLALVSVSDNDYSFYLA 198
            PNM TQID F++ +   N  +    + SSLALVSV+ NDY+ +LA
Sbjct: 136 LPNMTTQIDYFQRVLAAGNI-YSPSDLPSSLALVSVAGNDYATFLA 175

[Close]

BLAST of Spg013060 vs. ExPASy Swiss-Prot
Match: Q8RWJ4 (GDSL esterase/lipase At2g36325 OS=Arabidopsis thaliana OX=3702 GN=At2g36325 PE=2 SV=2)

HSP 1 Score: 127.5 bits (319), Expect = 1.8e-28
Identity = 70/147 (47.62%), Postives = 88/147 (59.86%), Query Frame = 0


Query: 56  FRATKLYVFGDSYVDTGNIGISNFSSRKFPYGITFPGKPTGRFSDGRVLTDYTARYLGVK 115
           F+ TKL+VFGDSY DTGN       S +FP GITFPG PTGRFSDGRV TDY A+Y+GV+
Sbjct: 43  FKPTKLFVFGDSYADTGNTPFLIVPSWRFPNGITFPGIPTGRFSDGRVSTDYLAKYIGVR 102

Query: 116 APVPFGILKSLGIIRGRENGVNFAFGGTGVFNTSVSF-PNMRTQIDLFEKFIVLNNSWFF 175
            P+ +   K        + G+NFA+GG G F T     P    QID FE+ ++ N   + 
Sbjct: 103 TPITYKWGKYGRPRLAVKRGMNFAYGGAGAFETMFKLVPTASVQIDSFEQLLMRN--VYS 162

Query: 176 KKHVCSSLALVSVSDNDYSFYLARNGS 202
              + SS+A  S+  NDY  Y  RNGS
Sbjct: 163 PADLNSSVAFFSIIGNDYLTYDRRNGS 187

[Close]

BLAST of Spg013060 vs. ExPASy Swiss-Prot
Match: Q9LZS9 (GDSL esterase/lipase At5g03590 OS=Arabidopsis thaliana OX=3702 GN=At5g03590 PE=3 SV=2)

HSP 1 Score: 107.1 bits (266), Expect = 2.6e-22
Identity = 62/160 (38.75%), Postives = 93/160 (58.13%), Query Frame = 0


Query: 45  GSAGHRHLCRQFRATKLYVFGDSYVDTGNIGISNFSSRKFPYGITFPGKPTGRFSDGRVL 104
           G+ G     +  +  KL+VFG+SY DTGN+  +   S K PYGITFPGKP+GR+SDG   
Sbjct: 19  GAVGSNQPSQANKNPKLFVFGNSYADTGNMKPTAL-SWKLPYGITFPGKPSGRYSDGLTA 78

Query: 105 TDYTARYLGVKAPVPFGILKSLGIIRGREN-GVNFAFGGTGVFNTSVS-FPNMRTQIDLF 164
           TD+ A+ LG K P    + ++ G  + + N G+NFAFGG+ VF++ V   PN+ TQ+   
Sbjct: 79  TDFLAKQLGAKLPY---LWRTHGKKKVKLNRGMNFAFGGSEVFDSPVDRSPNISTQVGFL 138

Query: 165 EKFIVLNNSWFFKKHVCSSLALVSVSDNDYSFYLARNGSL 203
               +    +     + SS AL+S S  DY  ++ +N ++
Sbjct: 139 VNLALARRVYTIDGDLASSYALLSYSGTDYYGFIDQNPNM 174

[Close]

BLAST of Spg013060 vs. ExPASy Swiss-Prot
Match: Q9LZS8 (GDSL esterase/lipase At5g03600 OS=Arabidopsis thaliana OX=3702 GN=At5g03600 PE=3 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 9.8e-22
Identity = 55/154 (35.71%), Postives = 90/154 (58.44%), Query Frame = 0


Query: 48  GHRHLCRQFRATKLYVFGDSYVDTGNIGISNFSSRKFPYGITFPGKPTGRFSDGRVLTDY 107
           G   L R  +  KL+VFGDSY DTGN    +  +   PYGITFPGKP+GR+ DG + TD+
Sbjct: 2   GSNQLLRPRKVPKLFVFGDSYADTGNTK-RDTEAWAIPYGITFPGKPSGRYCDGLIATDF 61

Query: 108 TARYLGVKAPVPFGILKSLGIIRGRENGVNFAFGGTGVFNTSVS--FPNMRTQIDLFEKF 167
             + LG ++P    + ++ G  +G + G+NFAFGG+ + ++S +  FPN+  Q++     
Sbjct: 62  LEKVLGAESPY---LYRTHGRDKGLKRGMNFAFGGSKMLDSSPNSPFPNITAQVNFLVDL 121

Query: 168 IVLNNSWFFKKHVCSSLALVSVSDNDYSFYLARN 200
           ++      +     S ++L+S +  DY +Y+ +N
Sbjct: 122 VLAGR--VYGDITPSDVSLISYAGGDYIYYIDQN 149

[Close]

BLAST of Spg013060 vs. ExPASy TrEMBL
Match: A0A0A0KFB6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G452050 PE=3 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 1.2e-59
Identity = 125/182 (68.68%), Postives = 141/182 (77.47%), Query Frame = 0


Query: 37  TFFLWFQQGSAGHR-HLCRQFRATKLYVFGDSYVDTGNIGISNFSSRKFPYGITFPGKPT 96
           T FL  +QGS GH  +L R+F+  KLYVFGDSYVDTGNIGISNFSSRKFPYGITFPGKP+
Sbjct: 13  TVFLLSEQGSGGHNDNLLREFQPKKLYVFGDSYVDTGNIGISNFSSRKFPYGITFPGKPS 72

Query: 97  GRFSDGRVLTDYTARYLGVKAPVPFGILKSLGIIRGRE----NGVNFAFGGTGVFNTSVS 156
           GRFSDGRVLTDY ARYLG+K P+PF + K L   +GR+    NGVNFA+GGTGVFNTSVS
Sbjct: 73  GRFSDGRVLTDYVARYLGLKPPIPFRVFKDLKRRQGRKNKNINGVNFAYGGTGVFNTSVS 132

Query: 157 FPNMRTQIDLFEKFIVLNNSWFFKKHVCSSLALVSVSDNDYSFYLARNGSLQVISGTFSS 214
           FPNM TQIDLF KFI        +  + SS+ALVSVS NDYSFYLARNGSLQ   G   S
Sbjct: 133 FPNMTTQIDLFHKFI-------DETDIQSSIALVSVSGNDYSFYLARNGSLQGFKGFMIS 187

[Close]

BLAST of Spg013060 vs. ExPASy TrEMBL
Match: A0A1S3CHQ7 (GDSL esterase/lipase At5g03610-like OS=Cucumis melo OX=3656 GN=LOC103500888 PE=3 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 1.2e-59
Identity = 125/181 (69.06%), Postives = 141/181 (77.90%), Query Frame = 0


Query: 37  TFFLWFQQGSAGHRHLCRQFRATKLYVFGDSYVDTGNIGISNFSSRKFPYGITFPGKPTG 96
           T  L  +QGSAGH  L R+FR  KLYVFGDSYVDTGNIGISN+SSRK+PYGITFPGKP+G
Sbjct: 12  TILLLSEQGSAGHNDLIREFRPKKLYVFGDSYVDTGNIGISNYSSRKYPYGITFPGKPSG 71

Query: 97  RFSDGRVLTDYTARYLGVKAPVPFGIL-KSLGIIRGRE---NGVNFAFGGTGVFNTSVSF 156
           RFSDGRVLTDY ARYLG+K P+PF +L K L   +GR+   NGVNFA+GGTGVFNTSVSF
Sbjct: 72  RFSDGRVLTDYVARYLGLKPPIPFRVLFKDLKRRQGRKKNINGVNFAYGGTGVFNTSVSF 131

Query: 157 PNMRTQIDLFEKFIVLNNSWFFKKHVCSSLALVSVSDNDYSFYLARNGSLQVISGTFSSL 214
           PNM TQIDLF KFI        +  + SS+ALVSVS NDYSFYLARNGSLQ   G   S+
Sbjct: 132 PNMTTQIDLFHKFI-------DETDIQSSIALVSVSGNDYSFYLARNGSLQGFKGFMISV 185

[Close]

BLAST of Spg013060 vs. ExPASy TrEMBL
Match: A0A5D3CAQ7 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G001230 PE=3 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 3.6e-59
Identity = 122/166 (73.49%), Postives = 136/166 (81.93%), Query Frame = 0


Query: 43  QQGSAGHRHLCRQFRATKLYVFGDSYVDTGNIGISNFSSRKFPYGITFPGKPTGRFSDGR 102
           +QGSAGH  L R+FR  KLYVFGDSYVDTGNIGISN+SSRK+PYGITFPGKP+GRFSDGR
Sbjct: 19  EQGSAGHNDLIREFRPKKLYVFGDSYVDTGNIGISNYSSRKYPYGITFPGKPSGRFSDGR 78

Query: 103 VLTDYTARYLGVKAPVPFGIL-KSLGIIRGRE---NGVNFAFGGTGVFNTSVSFPNMRTQ 162
           VLTDY ARYLG+K P+PF +L K L   +GR+   NGVNFA+GGTGVFNTSVSFPNM TQ
Sbjct: 79  VLTDYVARYLGLKPPIPFRVLFKDLKRRQGRKKNINGVNFAYGGTGVFNTSVSFPNMTTQ 138

Query: 163 IDLFEKFIVLNNSWFFKKHVCSSLALVSVSDNDYSFYLARNGSLQV 205
           IDLF KFI        +  + SS+ALVSVS NDYSFYLARNGSLQV
Sbjct: 139 IDLFHKFI-------DETDIQSSIALVSVSGNDYSFYLARNGSLQV 177

[Close]

BLAST of Spg013060 vs. ExPASy TrEMBL
Match: A0A0A0KKG2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G452030 PE=3 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 4.4e-49
Identity = 106/169 (62.72%), Postives = 122/169 (72.19%), Query Frame = 0


Query: 44  QGSAG-------HRHLCRQFRATKLYVFGDSYVDTGNIGISNFSSRKFPYGITFPGKPTG 103
           QGSAG       HRH     R TKL+VFGDSYVDTGNI     S+ +FPYGITFPGKP+G
Sbjct: 10  QGSAGGFYGNLRHRHRSHPSRGTKLFVFGDSYVDTGNILFPFSSAEQFPYGITFPGKPSG 69

Query: 104 RFSDGRVLTDYTARYLGVKAPVPFGILKSLGIIRGRENGVNFAFGGTGVFNTSVSFPNMR 163
           RFSDGRVLTD+ A++LGVK+P+PF I   +G  R +E+G+NFAFGGTGVFNT V  PNM 
Sbjct: 70  RFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLKESGINFAFGGTGVFNTLVPLPNMT 129

Query: 164 TQIDLFEKFIVLNNSWFFKKHVCSSLALVSVSDNDYSFYLARNGSLQVI 206
           TQIDLFE+     +     + V  SLALVSVS NDYSFYLA NGS QVI
Sbjct: 130 TQIDLFEQLRDDESGLISNRDVHLSLALVSVSGNDYSFYLATNGSAQVI 178

[Close]

BLAST of Spg013060 vs. ExPASy TrEMBL
Match: A0A5A7SLZ4 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold541G001230 PE=3 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 1.1e-47
Identity = 105/183 (57.38%), Postives = 132/183 (72.13%), Query Frame = 0


Query: 37  TFFLWFQQGSAG-----HRHLCRQFRATKLYVFGDSYVDTGNIGISNFSSRKFPYGITFP 96
           T  L+  QGSAG       H   + RATKL+VFGDSYVDTGNI +   S+++FPYGITFP
Sbjct: 15  TVSLFSGQGSAGGYYSNRHHRSHRLRATKLFVFGDSYVDTGNILVPFSSAQQFPYGITFP 74

Query: 97  GKPTGRFSDGRVLTDYTARYLGVKAPVPFGILKSLGIIRGRENGVNFAFGGTGVFNTSVS 156
           GKP+GRFSDGRVLTD+  ++LGVK+P+PF I   +G  R +E+G+NFAFGGTGVF+TSV 
Sbjct: 75  GKPSGRFSDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESGINFAFGGTGVFDTSVP 134

Query: 157 FPNMRTQIDLFEKFIVLNNSWFFKKHVCSSLALVSVSDNDYSFYLARNGSLQVISGTFSS 215
            PNM TQIDLFE+ +    S    + V  S+ALVSVS NDYSFYLA NGS Q +    +S
Sbjct: 135 LPNMTTQIDLFEQ-LRHAESGLSNRDVHLSVALVSVSGNDYSFYLATNGSAQGLKPFINS 194

[Close]

BLAST of Spg013060 vs. TAIR 10
Match: AT5G03610.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 166.4 bits (420), Expect = 2.5e-41
Identity = 84/156 (53.85%), Postives = 110/156 (70.51%), Query Frame = 0


Query: 44  QGSAGHRHLCRQFRATKLYVFGDSYVDTGNIGISNFSSRKFPYGITFPGKPTGRFSDGRV 103
           +GS  + HL   FR TKL+VFGDSY DTGNI  +  SS KFPYGITFPGKP GRFSDGRV
Sbjct: 28  EGSNQNHHL-YPFRPTKLFVFGDSYADTGNIKKAFSSSWKFPYGITFPGKPAGRFSDGRV 87

Query: 104 LTDYTARYLGVKAPVPFGILKSLGIIRGRENGVNFAFGGTGVFNTSVSFPNMRTQIDLFE 163
            TD+ A+++G+K+P+P+   K     +  + G+NFA+GGTGVFNT    PNM TQID+F+
Sbjct: 88  ATDFLAKFVGIKSPIPY-FWKDYAGKKRLQYGMNFAYGGTGVFNTQTPLPNMTTQIDIFQ 147

Query: 164 KFIVLNNSWFFKKHVCSSLALVSVSDNDYSFYLARN 200
             I+     ++   + SS+ALVSV+ NDYS ++A N
Sbjct: 148 N-ILTTGDIYYPPELTSSVALVSVAGNDYSNFIALN 180

[Close]

BLAST of Spg013060 vs. TAIR 10
Match: AT3G09930.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 148.3 bits (373), Expect = 7.1e-36
Identity = 82/166 (49.40%), Postives = 110/166 (66.27%), Query Frame = 0


Query: 36  ATFFLWFQQGSAGHRHLCRQFRATKLYVFGDSYVDTGNIGISNFSSRKFPYGITFPGKPT 95
           ++FFL  +     H +L ++ R  +L+VFGDSY DTGNI  S   S K PYGITFP KP+
Sbjct: 16  SSFFLGAESDYPQHSNL-KRLRPNRLFVFGDSYADTGNIRKSLSDSWKIPYGITFPQKPS 75

Query: 96  GRFSDGRVLTDYTARYLGVKAPVPFGILKSLGIIRGREN---GVNFAFGGTGVFNTSVS- 155
           GRFSDGRV TD+ ARYLG+K+P+P+    +     G+E    G+N+A+GGTGVF T  + 
Sbjct: 76  GRFSDGRVATDFLARYLGIKSPIPY----TWKDYAGKERLLYGMNYAYGGTGVFKTKDNP 135

Query: 156 FPNMRTQIDLFEKFIVLNNSWFFKKHVCSSLALVSVSDNDYSFYLA 198
            PNM TQID F++ +   N  +    + SSLALVSV+ NDY+ +LA
Sbjct: 136 LPNMTTQIDYFQRVLAAGNI-YSPSDLPSSLALVSVAGNDYATFLA 175

[Close]

BLAST of Spg013060 vs. TAIR 10
Match: AT2G36325.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 127.5 bits (319), Expect = 1.3e-29
Identity = 70/147 (47.62%), Postives = 88/147 (59.86%), Query Frame = 0


Query: 56  FRATKLYVFGDSYVDTGNIGISNFSSRKFPYGITFPGKPTGRFSDGRVLTDYTARYLGVK 115
           F+ TKL+VFGDSY DTGN       S +FP GITFPG PTGRFSDGRV TDY A+Y+GV+
Sbjct: 43  FKPTKLFVFGDSYADTGNTPFLIVPSWRFPNGITFPGIPTGRFSDGRVSTDYLAKYIGVR 102

Query: 116 APVPFGILKSLGIIRGRENGVNFAFGGTGVFNTSVSF-PNMRTQIDLFEKFIVLNNSWFF 175
            P+ +   K        + G+NFA+GG G F T     P    QID FE+ ++ N   + 
Sbjct: 103 TPITYKWGKYGRPRLAVKRGMNFAYGGAGAFETMFKLVPTASVQIDSFEQLLMRN--VYS 162

Query: 176 KKHVCSSLALVSVSDNDYSFYLARNGS 202
              + SS+A  S+  NDY  Y  RNGS
Sbjct: 163 PADLNSSVAFFSIIGNDYLTYDRRNGS 187

[Close]

BLAST of Spg013060 vs. TAIR 10
Match: AT5G03600.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 105.1 bits (261), Expect = 6.9e-23
Identity = 55/154 (35.71%), Postives = 90/154 (58.44%), Query Frame = 0


Query: 48  GHRHLCRQFRATKLYVFGDSYVDTGNIGISNFSSRKFPYGITFPGKPTGRFSDGRVLTDY 107
           G   L R  +  KL+VFGDSY DTGN    +  +   PYGITFPGKP+GR+ DG + TD+
Sbjct: 2   GSNQLLRPRKVPKLFVFGDSYADTGNTK-RDTEAWAIPYGITFPGKPSGRYCDGLIATDF 61

Query: 108 TARYLGVKAPVPFGILKSLGIIRGRENGVNFAFGGTGVFNTSVS--FPNMRTQIDLFEKF 167
             + LG ++P    + ++ G  +G + G+NFAFGG+ + ++S +  FPN+  Q++     
Sbjct: 62  LEKVLGAESPY---LYRTHGRDKGLKRGMNFAFGGSKMLDSSPNSPFPNITAQVNFLVDL 121

Query: 168 IVLNNSWFFKKHVCSSLALVSVSDNDYSFYLARN 200
           ++      +     S ++L+S +  DY +Y+ +N
Sbjct: 122 VLAGR--VYGDITPSDVSLISYAGGDYIYYIDQN 149

[Close]

BLAST of Spg013060 vs. TAIR 10
Match: AT1G53920.1 (GDSL-motif lipase 5 )

HSP 1 Score: 90.5 bits (223), Expect = 1.8e-18
Identity = 60/156 (38.46%), Postives = 83/156 (53.21%), Query Frame = 0


Query: 59  TKLYVFGDSYVDTGN---IGISNFSSRKF-PYGITFPGKPTGRFSDGRVLTDYTARYLGV 118
           T L++FGDS++D GN   I  +      F PYG TF G PTGRFSDGR+++D+ A Y  +
Sbjct: 47  TALFLFGDSFLDAGNNNYINTTTLDQANFPPYGQTFFGLPTGRFSDGRLISDFIAEYANL 106

Query: 119 KAPVPFGILKSLGIIRGRENGVNFAFGGTG--VFNTSVSFPNMRTQIDLFEKFIVLNNSW 178
               PF      G  + +  GVNFA  G G  V     S  N+RTQ+D ++K   L  + 
Sbjct: 107 PLIPPF---LEPGNSQKKLYGVNFASAGAGALVETFQGSVINLRTQLDHYKKVERLWRTN 166

Query: 179 F----FKKHVCSSLALVSVSDNDYSFYLARNGSLQV 205
           F     KK +  ++ L+S+  NDYS     N SL +
Sbjct: 167 FGKEESKKRISRAVYLISIGSNDYSSIFLTNQSLPI 199

The following BLAST results are available for this feature:

BLAST of Spg013060 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008462556.1	2.5e-59	69.06	PREDICTED: GDSL esterase/lipase At5g03610-like [Cucumis melo]	[more]
KAA0025269.1	7.4e-59	73.49	GDSL esterase/lipase [Cucumis melo var. makuwa] >TYK07399.1 GDSL esterase/lipase...	[more]
KAE8647419.1	2.1e-58	68.33	hypothetical protein Csa_004424 [Cucumis sativus]	[more]
XP_031742898.1	6.9e-57	65.61	GDSL esterase/lipase At5g03610 [Cucumis sativus]	[more]
XP_004143379.2	1.5e-48	57.84	GDSL esterase/lipase At5g03610 [Cucumis sativus] >KGN48533.2 hypothetical protei...	[more]

BLAST of Spg013060 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LZS7	3.6e-40	53.85	GDSL esterase/lipase At5g03610 OS=Arabidopsis thaliana OX=3702 GN=At5g03610 PE=2...	[more]
Q9SF94	1.0e-34	49.40	GDSL esterase/lipase At3g09930 OS=Arabidopsis thaliana OX=3702 GN=At3g09930 PE=2...	[more]
Q8RWJ4	1.8e-28	47.62	GDSL esterase/lipase At2g36325 OS=Arabidopsis thaliana OX=3702 GN=At2g36325 PE=2...	[more]
Q9LZS9	2.6e-22	38.75	GDSL esterase/lipase At5g03590 OS=Arabidopsis thaliana OX=3702 GN=At5g03590 PE=3...	[more]
Q9LZS8	9.8e-22	35.71	GDSL esterase/lipase At5g03600 OS=Arabidopsis thaliana OX=3702 GN=At5g03600 PE=3...	[more]

BLAST of Spg013060 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KFB6	1.2e-59	68.68	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G452050 PE=3 SV=1	[more]
A0A1S3CHQ7	1.2e-59	69.06	GDSL esterase/lipase At5g03610-like OS=Cucumis melo OX=3656 GN=LOC103500888 PE=3...	[more]
A0A5D3CAQ7	3.6e-59	73.49	GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202...	[more]
A0A0A0KKG2	4.4e-49	62.72	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G452030 PE=3 SV=1	[more]
A0A5A7SLZ4	1.1e-47	57.38	GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold541...	[more]

BLAST of Spg013060 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G03610.1	2.5e-41	53.85	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT3G09930.1	7.1e-36	49.40	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT2G36325.1	1.3e-29	47.62	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT5G03600.1	6.9e-23	35.71	SGNH hydrolase-type esterase superfamily protein	[more]
AT1G53920.1	1.8e-18	38.46	GDSL-motif lipase 5	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036514	SGNH hydrolase superfamily	GENE3D	3.40.50.1110	SGNH hydrolase	coord: 48..213 e-value: 4.8E-22 score: 80.8
IPR001087	GDSL lipase/esterase	PFAM	PF00657	Lipase_GDSL	coord: 61..118 e-value: 1.1E-9 score: 39.1
None	No IPR available	PANTHER	PTHR46020	OSJNBB0059K02.9 PROTEIN	coord: 41..207
None	No IPR available	PANTHER	PTHR46020:SF4	OSJNBB0059K02.9 PROTEIN	coord: 41..207

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg013060.1	Spg013060.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds