Spg013029 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg013029
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	L-ascorbate oxidase
Location	scaffold1: 6541889 .. 6544387 (-)
RNA-Seq Expression	Spg013029
Synteny	Spg013029

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATTTTCAGCCTTTTGGGTTTCGAAACAAGTACTAAAACGATTCAACAAATGGCTTCTCTCTTACAGGGGAGACAAATCCTATTAATTTTTCTCTGTTTCTTCGTGTTCTTATTGGAATCTCAAACTGCAGAATCTAGAACTCTGCATTATAAATGGGAAGTAAAGTCCGAGTTGAAGTCCCCAGACTGCTTCAAAAAGCTCACCATGACCATTAATGGCCGATCTCCAGGTCCCACAATCTACGCCCACCAAGGCGACACCGTCATCGTCGAGCTCAAGAACAGCTTCGGTACCGAAAATGTCGCTATCCACTGGCATGGAATCCGACAGGTACTCCAAGTTTCATATGTCTTCAAATACTAACACATCAGCTCATTTTGTATGTTTTTGTATTGGGTTGATTTTTTTTTTTTTTTTTTTTTTTTGGTTCTAATGTGTTTGGATTTTTGTGTAATAATTGAAGATCGGAACACCTTGGAGCGATGGAACAGAAGGTGTCACTCAATGTCCTATTTTGCCTGGTGAAACTTTCAAATACCAGTTCGTTGTGGATAGAGTAAGAACTGGACCCACTTATTTTTTTCTTTTTGAGGGGGGCCTCTGAAAATAGTTTTGGCCTTATGGGCTATAAAAAGAAATCTTTTTAACGAATAATTTGTCATTGATCTTGTCAATTTTGAAATCTGCAGCCAGGGACATACCTATACCATGCTCATTACGGTATGCAAAGAGAAGATGGTCTCTATGGATCCATCGTGGTTTGGCTTCCAGAAGGACAATCAGAGCCCTTCTCCTACGATCATGATCGGAATCTCATCCTCACCGATTGGTATCATAAAAGCAGCCATGAACACGCCGCCGGATTGGCTACTCCCAACTCTGGCTTCCAATGGGTCGGAGAACCTGATGTAAATTCAAACTCCCATCATTAACTTTCCACTATTTAACTGAAATTGATTCGTTTACTAATGGGTTTCTTCTTATTTGTTGCTCTCTCAGTCACTTTTGATACAAGGAAGGGGAAGATTCAATTGCTCTCTGTTGGGTTCTTCCAGTTTAGCTTCTGGTACTTGCAACTCATCCAGTCCAGATTGTGTTCCTTATCCACTAACAGTCGTACCAGGAAAAACCTACCGATTGAGGGTCTCAAGTCTCACTTCTCTTTCAGCCCTCAGTTTCCAAATTGAGGTAACCATATAGTTAATTTCACTCGCCTCGCTCTAATTTCTCGAAATTCATCAGAAATGACTCAAACTCTCTGTTTCGGAAACAGGGTCACACCATGACTGTGGTTGAAGCAGATGGACACTACGTCGAGCCATTCCAAGTAAACACCTTGTTCATATACTCAGGAGAAACATATTCCGTGTTGATTAAAGCCGACCAAGACCCTTCTAGAAACTACTGGATCACAACCAATGTGGTTGGCCGGAACAGAACCACACCCCCTGGTTTAGCCATTTTCAATTACTACCCAAATCACCAACGTAAATTGCCTCCGACAACTCCTCCCGCGGGACCTGCCTGGAACGACGTTGCACCCCGTTTGGCTCAAAGCCACGCCATCAAAGCCCGACAAGGCTTCATCCACGCGCCACCCAAATCTGCTGACAAAGTGATCGTGTTACTCAACACACAAAACACCGTCAATGGACGTCGCCGGTGGTCGTTAAACAATGTGTCGTTCAACATGCCCCACACCCCTTACCTTATTGCCCTGAAGAACAATCTTCTCCACACCTTCAGCCAGGCCAAACCACCCACTGGATACGACTTCAAGACTTACGACATTTTCAATCCACCGCCCAACCCTGAAGCGACTGTGAGCGACGCCATTTATCGATTGGATTTCAATTCAACGGTGGATATAATTCTGCAGAACGCCAACATGATGCGGCCAAACAACAGCGAAACGCATCCTTGGCATTTGCACGGCCACGATTTCTGGGTGTTGGGATACGGTGAAGGGAAGTTCGATATGTATAAAGATCCAAGCAAGTACAATTTGGAGAATCCGATCATGAAGAACACCGTGCCATTGCACCCTTTCGGATGGACGGCTTTGAGATTTGTGGCTGACAATCCTGGAGTTTGGGCCTTCCATTGCCATATCGACGCCCATTTCTTTATGGGAATGGGTGTTGTTTTTGAAGAAGGGATTGAAAAGGTTGGGAAATTGCCTACTTCGATTATGGGGTGCGGCGAGAGCAAGAGGTTCATAAGGCCTTAAGTCACTTCAATGAGGATCTGTTTAAGTCCCCATTATTCTTTGATATCAGGATTGATGGCTGATATTACCCAACAAGTTGCAGAAAAGTCTATTCTATATGTTTAGCCATATTCTTTACAACAATAAAGTCAATATGAGGGTATACGTAAATGTTATTCTATTCTTTATTATAATAATTGTTTTTTTTTTAGTTTTTTATTATAATAATTGTAATCGTTTTTCCCTACAATCGAAATTGTAAAATTTATTGAAGTACGAATAAAATT

mRNA sequence

ATGATTTTCAGCCTTTTGGGTTTCGAAACAAGTACTAAAACGATTCAACAAATGGCTTCTCTCTTACAGGGGAGACAAATCCTATTAATTTTTCTCTGTTTCTTCGTGTTCTTATTGGAATCTCAAACTGCAGAATCTAGAACTCTGCATTATAAATGGGAAGTAAAGTCCGAGTTGAAGTCCCCAGACTGCTTCAAAAAGCTCACCATGACCATTAATGGCCGATCTCCAGGTCCCACAATCTACGCCCACCAAGGCGACACCGTCATCGTCGAGCTCAAGAACAGCTTCGGTACCGAAAATGTCGCTATCCACTGGCATGGAATCCGACAGATCGGAACACCTTGGAGCGATGGAACAGAAGGTGTCACTCAATGTCCTATTTTGCCTGGTGAAACTTTCAAATACCAGTTCGTTGTGGATAGACCAGGGACATACCTATACCATGCTCATTACGGTATGCAAAGAGAAGATGGTCTCTATGGATCCATCGTGGTTTGGCTTCCAGAAGGACAATCAGAGCCCTTCTCCTACGATCATGATCGGAATCTCATCCTCACCGATTGGTATCATAAAAGCAGCCATGAACACGCCGCCGGATTGGCTACTCCCAACTCTGGCTTCCAATGGGTCGGAGAACCTGATTCACTTTTGATACAAGGAAGGGGAAGATTCAATTGCTCTCTGTTGGGTTCTTCCAGTTTAGCTTCTGGTACTTGCAACTCATCCAGTCCAGATTGTGTTCCTTATCCACTAACAGTCGTACCAGGAAAAACCTACCGATTGAGGGTCTCAAGTCTCACTTCTCTTTCAGCCCTCAGTTTCCAAATTGAGGGTCACACCATGACTGTGGTTGAAGCAGATGGACACTACGTCGAGCCATTCCAAGTAAACACCTTGTTCATATACTCAGGAGAAACATATTCCGTGTTGATTAAAGCCGACCAAGACCCTTCTAGAAACTACTGGATCACAACCAATGTGGTTGGCCGGAACAGAACCACACCCCCTGGTTTAGCCATTTTCAATTACTACCCAAATCACCAACGTAAATTGCCTCCGACAACTCCTCCCGCGGGACCTGCCTGGAACGACGTTGCACCCCGTTTGGCTCAAAGCCACGCCATCAAAGCCCGACAAGGCTTCATCCACGCGCCACCCAAATCTGCTGACAAAGTGATCGTGTTACTCAACACACAAAACACCGTCAATGGACGTCGCCGGTGGTCGTTAAACAATGTGTCGTTCAACATGCCCCACACCCCTTACCTTATTGCCCTGAAGAACAATCTTCTCCACACCTTCAGCCAGGCCAAACCACCCACTGGATACGACTTCAAGACTTACGACATTTTCAATCCACCGCCCAACCCTGAAGCGACTGTGAGCGACGCCATTTATCGATTGGATTTCAATTCAACGGTGGATATAATTCTGCAGAACGCCAACATGATGCGGCCAAACAACAGCGAAACGCATCCTTGGCATTTGCACGGCCACGATTTCTGGGTGTTGGGATACGGTGAAGGGAAGTTCGATATGTATAAAGATCCAAGCAAGTACAATTTGGAGAATCCGATCATGAAGAACACCGTGCCATTGCACCCTTTCGGATGGACGGCTTTGAGATTTGTGGCTGACAATCCTGGAGTTTGGGCCTTCCATTGCCATATCGACGCCCATTTCTTTATGGGAATGGGTGTTGTTTTTGAAGAAGGGATTGAAAAGGTTGGGAAATTGCCTACTTCGATTATGGGGTGCGGCGAGAGCAAGAGGTTCATAAGGCCTTAA

Coding sequence (CDS)

ATGATTTTCAGCCTTTTGGGTTTCGAAACAAGTACTAAAACGATTCAACAAATGGCTTCTCTCTTACAGGGGAGACAAATCCTATTAATTTTTCTCTGTTTCTTCGTGTTCTTATTGGAATCTCAAACTGCAGAATCTAGAACTCTGCATTATAAATGGGAAGTAAAGTCCGAGTTGAAGTCCCCAGACTGCTTCAAAAAGCTCACCATGACCATTAATGGCCGATCTCCAGGTCCCACAATCTACGCCCACCAAGGCGACACCGTCATCGTCGAGCTCAAGAACAGCTTCGGTACCGAAAATGTCGCTATCCACTGGCATGGAATCCGACAGATCGGAACACCTTGGAGCGATGGAACAGAAGGTGTCACTCAATGTCCTATTTTGCCTGGTGAAACTTTCAAATACCAGTTCGTTGTGGATAGACCAGGGACATACCTATACCATGCTCATTACGGTATGCAAAGAGAAGATGGTCTCTATGGATCCATCGTGGTTTGGCTTCCAGAAGGACAATCAGAGCCCTTCTCCTACGATCATGATCGGAATCTCATCCTCACCGATTGGTATCATAAAAGCAGCCATGAACACGCCGCCGGATTGGCTACTCCCAACTCTGGCTTCCAATGGGTCGGAGAACCTGATTCACTTTTGATACAAGGAAGGGGAAGATTCAATTGCTCTCTGTTGGGTTCTTCCAGTTTAGCTTCTGGTACTTGCAACTCATCCAGTCCAGATTGTGTTCCTTATCCACTAACAGTCGTACCAGGAAAAACCTACCGATTGAGGGTCTCAAGTCTCACTTCTCTTTCAGCCCTCAGTTTCCAAATTGAGGGTCACACCATGACTGTGGTTGAAGCAGATGGACACTACGTCGAGCCATTCCAAGTAAACACCTTGTTCATATACTCAGGAGAAACATATTCCGTGTTGATTAAAGCCGACCAAGACCCTTCTAGAAACTACTGGATCACAACCAATGTGGTTGGCCGGAACAGAACCACACCCCCTGGTTTAGCCATTTTCAATTACTACCCAAATCACCAACGTAAATTGCCTCCGACAACTCCTCCCGCGGGACCTGCCTGGAACGACGTTGCACCCCGTTTGGCTCAAAGCCACGCCATCAAAGCCCGACAAGGCTTCATCCACGCGCCACCCAAATCTGCTGACAAAGTGATCGTGTTACTCAACACACAAAACACCGTCAATGGACGTCGCCGGTGGTCGTTAAACAATGTGTCGTTCAACATGCCCCACACCCCTTACCTTATTGCCCTGAAGAACAATCTTCTCCACACCTTCAGCCAGGCCAAACCACCCACTGGATACGACTTCAAGACTTACGACATTTTCAATCCACCGCCCAACCCTGAAGCGACTGTGAGCGACGCCATTTATCGATTGGATTTCAATTCAACGGTGGATATAATTCTGCAGAACGCCAACATGATGCGGCCAAACAACAGCGAAACGCATCCTTGGCATTTGCACGGCCACGATTTCTGGGTGTTGGGATACGGTGAAGGGAAGTTCGATATGTATAAAGATCCAAGCAAGTACAATTTGGAGAATCCGATCATGAAGAACACCGTGCCATTGCACCCTTTCGGATGGACGGCTTTGAGATTTGTGGCTGACAATCCTGGAGTTTGGGCCTTCCATTGCCATATCGACGCCCATTTCTTTATGGGAATGGGTGTTGTTTTTGAAGAAGGGATTGAAAAGGTTGGGAAATTGCCTACTTCGATTATGGGGTGCGGCGAGAGCAAGAGGTTCATAAGGCCTTAA

Protein sequence

MIFSLLGFETSTKTIQQMASLLQGRQILLIFLCFFVFLLESQTAESRTLHYKWEVKSELKSPDCFKKLTMTINGRSPGPTIYAHQGDTVIVELKNSFGTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFKYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSHEHAAGLATPNSGFQWVGEPDSLLIQGRGRFNCSLLGSSSLASGTCNSSSPDCVPYPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNTLFIYSGETYSVLIKADQDPSRNYWITTNVVGRNRTTPPGLAIFNYYPNHQRKLPPTTPPAGPAWNDVAPRLAQSHAIKARQGFIHAPPKSADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKPPTGYDFKTYDIFNPPPNPEATVSDAIYRLDFNSTVDIILQNANMMRPNNSETHPWHLHGHDFWVLGYGEGKFDMYKDPSKYNLENPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIEKVGKLPTSIMGCGESKRFIRP

Homology

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BLAST of Spg013029 vs. NCBI nr
Match: XP_038881120.1 (L-ascorbate oxidase [Benincasa hispida])

HSP 1 Score: 1162.5 bits (3006), Expect = 0.0e+00
Identity = 547/596 (91.78%), Postives = 570/596 (95.64%), Query Frame = 0


Query: 1   MIFSLLGFETSTKTIQQMASLLQGRQILLIFLCFFVFLLESQTAESRTLHYKWEVKSELK 60
           M FSLLGF  STKTIQQMASLLQ RQ LLI LCF +FL++S  AESRTL+YKWEVKSELK
Sbjct: 1   MSFSLLGFGRSTKTIQQMASLLQSRQYLLILLCFLLFLIQSPIAESRTLNYKWEVKSELK 60

Query: 61  SPDCFKKLTMTINGRSPGPTIYAHQGDTVIVELKNSFGTENVAIHWHGIRQIGTPWSDGT 120
           SPDCFKKLTMTINGRSPGPTIYAHQGDTVIVELKNSF TENVAIHWHGIRQIGTPWSDGT
Sbjct: 61  SPDCFKKLTMTINGRSPGPTIYAHQGDTVIVELKNSFSTENVAIHWHGIRQIGTPWSDGT 120

Query: 121 EGVTQCPILPGETFKYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQSEPFSYDH 180
           EGVTQCPILPGETFKYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQ+EPFSYD+
Sbjct: 121 EGVTQCPILPGETFKYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFSYDY 180

Query: 181 DRNLILTDWYHKSSHEHAAGLATPNSGFQWVGEPDSLLIQGRGRFNCSLLGSSSLASGTC 240
           DRNLILTDWYHK+SHEHAAGLATP SGF WVGEPDSLLIQGRGRFNCS+LGSS+  S TC
Sbjct: 181 DRNLILTDWYHKTSHEHAAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSST--SATC 240

Query: 241 NSSSPDCVPYPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNTL 300
           NSS+P+C+ YP+TVVPGKTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVN L
Sbjct: 241 NSSNPECILYPITVVPGKTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNL 300

Query: 301 FIYSGETYSVLIKADQDPSRNYWITTNVVGRNRTTPPGLAIFNYYPNHQRKLPPTTPPAG 360
           FIYSGETYSVLIKADQDPSRNYWITTNVV R+RTTPPGLAIFNYYPNHQRKLPPTTPPA 
Sbjct: 301 FIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAIFNYYPNHQRKLPPTTPPAP 360

Query: 361 PAWNDVAPRLAQSHAIKARQGFIHAPPKSADKVIVLLNTQNTVNGRRRWSLNNVSFNMPH 420
           PAWNDVAPRLAQSHAIKARQGFIHAPPK+ADKVIVLLNTQNTVNGRRRWSLNNVSFNMPH
Sbjct: 361 PAWNDVAPRLAQSHAIKARQGFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPH 420

Query: 421 TPYLIALKNNLLHTFSQAKPPTGYDFKTYDIFNPPPNPEATVSDAIYRLDFNSTVDIILQ 480
           TPYLIALK+NLLH FSQ KPPTGYDFK YDIFNP PNPEATVSDAI+RLDFNSTVDIILQ
Sbjct: 421 TPYLIALKHNLLHAFSQDKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQ 480

Query: 481 NANMMRPNNSETHPWHLHGHDFWVLGYGEGKFDMYKDPSKYNLENPIMKNTVPLHPFGWT 540
           NANMM PNNSETHPWHLHGHDFWVLGYGEGKFD+YKDPSK+NLENPIMKNTVPLHP+GWT
Sbjct: 481 NANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYKDPSKFNLENPIMKNTVPLHPYGWT 540

Query: 541 ALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIEKVGKLPTSIMGCGESKRFIRP 597
           ALRFVADNPGVWAFHCHIDAHFFMGMGVVF EGI++V KLPTSIMGCGESKRF+RP
Sbjct: 541 ALRFVADNPGVWAFHCHIDAHFFMGMGVVFAEGIDRVQKLPTSIMGCGESKRFLRP 594

[Close]

BLAST of Spg013029 vs. NCBI nr
Match: KAG6594863.1 (L-ascorbate oxidase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026826.1 L-ascorbate oxidase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1128.6 bits (2918), Expect = 0.0e+00
Identity = 534/579 (92.23%), Postives = 548/579 (94.65%), Query Frame = 0


Query: 18  MASLLQGRQILLIFLCFFVFLLESQTAESRTLHYKWEVKSELKSPDCFKKLTMTINGRSP 77
           M SLLQ RQ LLI LCFFVFL ES+TAESRTL+YKWEVKSELKSPDCFKKLT+TINGRSP
Sbjct: 1   MGSLLQSRQYLLILLCFFVFLWESRTAESRTLYYKWEVKSELKSPDCFKKLTLTINGRSP 60

Query: 78  GPTIYAHQGDTVIVELKNSFGTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFKYQ 137
           GPTIYAHQGDTVIVELKNSF TENVAIHWHGIRQIGTPWSDGTEGVTQCP+LPGETFKYQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQ 120

Query: 138 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSHEH 197
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQ+EPF+YDHDRNLILTDWYHKSSHEH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEH 180

Query: 198 AAGLATPNSGFQWVGEPDSLLIQGRGRFNCSLLGSSSLASGTCNSSSPDCVPYPLTVVPG 257
           +AGLA   S F WVGEPDSLLIQGRGRFNCSLLG    AS TCNSSSPDCV YP+TVVPG
Sbjct: 181 SAGLAAAGSAFTWVGEPDSLLIQGRGRFNCSLLG----ASATCNSSSPDCVLYPITVVPG 240

Query: 258 KTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNTLFIYSGETYSVLIKADQD 317
           +TYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQV+ LFIYSGETYSVLIKADQ+
Sbjct: 241 RTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQN 300

Query: 318 PSRNYWITTNVVGRNRTTPPGLAIFNYYPNHQRKLPPTTPPAGPAWNDVAPRLAQSHAIK 377
           PSRNYWITTNVV RN TTP GLAIFNYYPNHQRK PPTTPPAGPAWN VAPRLAQSHAIK
Sbjct: 301 PSRNYWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIK 360

Query: 378 ARQGFIHAPPKSADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQ 437
           ARQGFIH PPK+ADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQ
Sbjct: 361 ARQGFIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQ 420

Query: 438 AKPPTGYDFKTYDIFNPPPNPEATVSDAIYRLDFNSTVDIILQNANMMRPNNSETHPWHL 497
           AKPPTGYDFK Y+IF PPPNPEATVSDAIYRLDFNSTVDIILQNANMM  N SETHPWHL
Sbjct: 421 AKPPTGYDFKNYNIFVPPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHL 480

Query: 498 HGHDFWVLGYGEGKFDMYKDPSKYNLENPIMKNTVPLHPFGWTALRFVADNPGVWAFHCH 557
           HGHDFWVLGYGEGKFDM+KDPSKYNL NPIMKNTVPLHPFGWTALRFVADNPGVWAFHCH
Sbjct: 481 HGHDFWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCH 540

Query: 558 IDAHFFMGMGVVFEEGIEKVGKLPTSIMGCGESKRFIRP 597
           IDAHFFMGMGVVFEEGIEKV KLPTSIMGCGESKRFIRP
Sbjct: 541 IDAHFFMGMGVVFEEGIEKVQKLPTSIMGCGESKRFIRP 575

[Close]

BLAST of Spg013029 vs. NCBI nr
Match: XP_008440636.1 (PREDICTED: L-ascorbate oxidase [Cucumis melo] >KAA0036280.1 L-ascorbate oxidase [Cucumis melo var. makuwa] >TYK12674.1 L-ascorbate oxidase [Cucumis melo var. makuwa])

HSP 1 Score: 1126.3 bits (2912), Expect = 0.0e+00
Identity = 530/579 (91.54%), Postives = 552/579 (95.34%), Query Frame = 0


Query: 18  MASLLQGRQILLIFLCFFVFLLESQTAESRTLHYKWEVKSELKSPDCFKKLTMTINGRSP 77
           MASLLQ +Q  L+ L  F+FLL+SQTAESRTL+YKWEVKSELKSPDCFKKLT+TINGRSP
Sbjct: 1   MASLLQRKQYFLLLLSSFLFLLQSQTAESRTLNYKWEVKSELKSPDCFKKLTITINGRSP 60

Query: 78  GPTIYAHQGDTVIVELKNSFGTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFKYQ 137
           GPTIYAHQGDTVIVELKNS  TENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETF+YQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSLHTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120

Query: 138 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSHEH 197
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVW+PEGQ+EPFSYDHDRNLILTDWYHKSS+EH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDHDRNLILTDWYHKSSNEH 180

Query: 198 AAGLATPNSGFQWVGEPDSLLIQGRGRFNCSLLGSSSLASGTCNSSSPDCVPYPLTVVPG 257
           AAGLATP SGF WVGEPDSLLIQGRGRFNCS+LGSS   SGTCNSSS +CV YPLTVVPG
Sbjct: 181 AAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSS---SGTCNSSSTECVLYPLTVVPG 240

Query: 258 KTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNTLFIYSGETYSVLIKADQD 317
           KTYRLRVSSLTSLSALSFQIEGH+MTVVEADGHYVEPFQVN LFIYSGETYSVLIKADQD
Sbjct: 241 KTYRLRVSSLTSLSALSFQIEGHSMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQD 300

Query: 318 PSRNYWITTNVVGRNRTTPPGLAIFNYYPNHQRKLPPTTPPAGPAWNDVAPRLAQSHAIK 377
           PSRNYWITTNVV R+RTTPPGLAIFNYYPNHQ K PPTTPPA PAWNDV PRLAQS AIK
Sbjct: 301 PSRNYWITTNVVSRSRTTPPGLAIFNYYPNHQSKRPPTTPPAPPAWNDVPPRLAQSQAIK 360

Query: 378 ARQGFIHAPPKSADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQ 437
           ARQGFIHAPPK+ADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALK+NLLHTFSQ
Sbjct: 361 ARQGFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQ 420

Query: 438 AKPPTGYDFKTYDIFNPPPNPEATVSDAIYRLDFNSTVDIILQNANMMRPNNSETHPWHL 497
            KPPTGYDFK YDIFNP PNPEATVSDAI+RLDFNSTVD+ILQNANMM PNNSETHPWHL
Sbjct: 421 EKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDLILQNANMMSPNNSETHPWHL 480

Query: 498 HGHDFWVLGYGEGKFDMYKDPSKYNLENPIMKNTVPLHPFGWTALRFVADNPGVWAFHCH 557
           HGHDFWVLGYGEGKFD+Y DPSKYNLENPIMKNTVPLHP+GWTALRFVADNPGVWAFHCH
Sbjct: 481 HGHDFWVLGYGEGKFDIYNDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCH 540

Query: 558 IDAHFFMGMGVVFEEGIEKVGKLPTSIMGCGESKRFIRP 597
           IDAHFFMGMGVVFEEGIE+VGKLPTSIMGCGESKRF+RP
Sbjct: 541 IDAHFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 576

[Close]

BLAST of Spg013029 vs. NCBI nr
Match: XP_023003749.1 (L-ascorbate oxidase [Cucurbita maxima])

HSP 1 Score: 1125.9 bits (2911), Expect = 0.0e+00
Identity = 532/579 (91.88%), Postives = 548/579 (94.65%), Query Frame = 0


Query: 18  MASLLQGRQILLIFLCFFVFLLESQTAESRTLHYKWEVKSELKSPDCFKKLTMTINGRSP 77
           M SLLQ RQ LLI LCFFVFL ES+TAESRTL+Y+WEVKSELKSPDCFKKLT+TINGRSP
Sbjct: 1   MGSLLQRRQYLLILLCFFVFLWESRTAESRTLYYRWEVKSELKSPDCFKKLTLTINGRSP 60

Query: 78  GPTIYAHQGDTVIVELKNSFGTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFKYQ 137
           GPTIYAHQGDTVIVELKNSF TENVAIHWHGIRQIGTPWSDGTEGVTQCP+LPGETFKYQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQ 120

Query: 138 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSHEH 197
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQ+EPF+YDHDRNLILTDWYHKSSHEH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEH 180

Query: 198 AAGLATPNSGFQWVGEPDSLLIQGRGRFNCSLLGSSSLASGTCNSSSPDCVPYPLTVVPG 257
           +AGLA P S F WVGEPDSLLIQGRGRFNCSL      AS TCNSSSPDCV YP+TVVPG
Sbjct: 181 SAGLAAPGSAFNWVGEPDSLLIQGRGRFNCSL-----GASATCNSSSPDCVLYPITVVPG 240

Query: 258 KTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNTLFIYSGETYSVLIKADQD 317
           +TYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQV+ LFIYSGETYSVLIKADQ+
Sbjct: 241 RTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQN 300

Query: 318 PSRNYWITTNVVGRNRTTPPGLAIFNYYPNHQRKLPPTTPPAGPAWNDVAPRLAQSHAIK 377
           PSRNYWITTNVV RN TTP GLAIFNYYPNHQRK PPTTPPAGPAWN VAPRLAQSHAIK
Sbjct: 301 PSRNYWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIK 360

Query: 378 ARQGFIHAPPKSADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQ 437
           ARQGFIH PPK+ADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQ
Sbjct: 361 ARQGFIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQ 420

Query: 438 AKPPTGYDFKTYDIFNPPPNPEATVSDAIYRLDFNSTVDIILQNANMMRPNNSETHPWHL 497
           AKPPTGYDFK Y+IF+PPPNPEATVSDAIYRLDFNSTVDIILQNANMM  N SETHPWHL
Sbjct: 421 AKPPTGYDFKNYNIFDPPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHL 480

Query: 498 HGHDFWVLGYGEGKFDMYKDPSKYNLENPIMKNTVPLHPFGWTALRFVADNPGVWAFHCH 557
           HGHDFWVLGYGEGKFDM+KDPSKYNL NPIMKNTVPLHPFGWTALRFVADNPGVWAFHCH
Sbjct: 481 HGHDFWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCH 540

Query: 558 IDAHFFMGMGVVFEEGIEKVGKLPTSIMGCGESKRFIRP 597
           IDAHFFMGMGVVFEEGIEKV KLPTSIMGCGESKRFIRP
Sbjct: 541 IDAHFFMGMGVVFEEGIEKVQKLPTSIMGCGESKRFIRP 574

[Close]

BLAST of Spg013029 vs. NCBI nr
Match: XP_004143482.1 (L-ascorbate oxidase [Cucumis sativus] >KGN48772.1 hypothetical protein Csa_003073 [Cucumis sativus])

HSP 1 Score: 1124.4 bits (2907), Expect = 0.0e+00
Identity = 530/579 (91.54%), Postives = 549/579 (94.82%), Query Frame = 0


Query: 18  MASLLQGRQILLIFLCFFVFLLESQTAESRTLHYKWEVKSELKSPDCFKKLTMTINGRSP 77
           MAS LQ  + LLI L F  FLL+SQTAESRTL+YKWEVKSELKSPDCFKK+T+TINGRSP
Sbjct: 1   MASPLQSTRYLLILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSP 60

Query: 78  GPTIYAHQGDTVIVELKNSFGTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFKYQ 137
           GPTIYAHQGDTVIVELKNS  TENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETF+YQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120

Query: 138 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSHEH 197
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVW+PEGQ+EPFSYD DRNLILTDWYHKSSHEH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEH 180

Query: 198 AAGLATPNSGFQWVGEPDSLLIQGRGRFNCSLLGSSSLASGTCNSSSPDCVPYPLTVVPG 257
           A GLATP SGF WVGEPDSLLIQGRGRFNCS+LGSS   SGTCNSSS +CV +PLTVVPG
Sbjct: 181 ATGLATPGSGFNWVGEPDSLLIQGRGRFNCSILGSS---SGTCNSSSTECVLHPLTVVPG 240

Query: 258 KTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNTLFIYSGETYSVLIKADQD 317
           KTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVN LFIYSGETYSVLIKADQD
Sbjct: 241 KTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQD 300

Query: 318 PSRNYWITTNVVGRNRTTPPGLAIFNYYPNHQRKLPPTTPPAGPAWNDVAPRLAQSHAIK 377
           PSRNYWITTNVV R+RTTPPGLAIFNYYPNHQ +LPPTTPPA PAWNDV PRLAQS AIK
Sbjct: 301 PSRNYWITTNVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIK 360

Query: 378 ARQGFIHAPPKSADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQ 437
           ARQGFIH PPK+ADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALK+NLLHTFSQ
Sbjct: 361 ARQGFIHTPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQ 420

Query: 438 AKPPTGYDFKTYDIFNPPPNPEATVSDAIYRLDFNSTVDIILQNANMMRPNNSETHPWHL 497
            KPPTGYDFK YDIFNP PNPEATVSDAIYRLDFNSTVDIILQNANMM PNNSETHPWHL
Sbjct: 421 EKPPTGYDFKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHL 480

Query: 498 HGHDFWVLGYGEGKFDMYKDPSKYNLENPIMKNTVPLHPFGWTALRFVADNPGVWAFHCH 557
           HGHDFWVLGYGEGKFD+YKDPSKYNLENPIMKNTVPLHP+GWTALRFVADNPGVWAFHCH
Sbjct: 481 HGHDFWVLGYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCH 540

Query: 558 IDAHFFMGMGVVFEEGIEKVGKLPTSIMGCGESKRFIRP 597
           IDAHFFMGMGVVFEEGIE+VGKLPTSIMGCGESKRF+RP
Sbjct: 541 IDAHFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 576

[Close]

BLAST of Spg013029 vs. ExPASy Swiss-Prot
Match: P24792 (L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2)

HSP 1 Score: 621.3 bits (1601), Expect = 1.1e-176
Identity = 292/563 (51.87%), Postives = 382/563 (67.85%), Query Frame = 0


Query: 27  ILLIFLCFFVFLLESQTAESRTLHYKWEVKSELKSPDCFKKLTMTINGRSPGPTIYAHQG 86
           IL  F     F + S+ ++ R  HYKWEV+    +PDC + + M ING+ PGPTI A+ G
Sbjct: 14  ILFFFGLILAFGISSEGSQIR--HYKWEVEYMFWAPDCNENIVMGINGQFPGPTIRANAG 73

Query: 87  DTVIVELKNSFGTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFKYQFVVDRPGTY 146
           DTV+VEL N   TE V IHWHGI Q GTPW+DGT  ++QC I PGETF Y F VD PGT+
Sbjct: 74  DTVVVELINKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFFYNFTVDNPGTF 133

Query: 147 LYHAHYGMQREDGLYGSIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSHEHAAGLATPNS 206
            YH H GMQR  GLYGS++V  P+G+ EPF YD + NL+L+DW+H+S H+   GL+  + 
Sbjct: 134 FYHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLS--SK 193

Query: 207 GFQWVGEPDSLLIQGRGRFNCSLLGSSSLASGTCN-SSSPDCVPYPLTVVPGKTYRLRVS 266
             +W+GEP ++L+ GRG+F+CS+          C    S  C PY   V+P KTYR+R++
Sbjct: 194 PIRWIGEPQTILLNGRGQFDCSIAAKYDSNLEPCKLKGSEPCAPYIFHVMPKKTYRIRIA 253

Query: 267 SLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNTLFIYSGETYSVLIKADQDPSRNYWIT 326
           S T+L+AL+F I  H + VVEADG+YV+PF  + + IYSGE+YSVLI  DQ+PS NYW++
Sbjct: 254 STTALAALNFAIGNHPLLVVEADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWVS 313

Query: 327 TNVVGRNRTTPPGLAIFNYYPNHQRKLPPTTPPAGPAWNDVAPRLAQSHAIKARQGFIHA 386
               GR+  TPPGL + NY PN   KLP + PP  PAW+D       ++ I A  G    
Sbjct: 314 VGTRGRHPNTPPGLTLLNYLPNSVSKLPTSPPPETPAWDDFDRSKNFTYRITAAMG-SPK 373

Query: 387 PPKSADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKPPTGYD 446
           PP  +++ I LLNTQN +NG  +W++N+VS  +P TPYL A+K NLLH F Q  PP  + 
Sbjct: 374 PPVKSNRRIFLLNTQNVINGYVKWAINDVSLALPPTPYLGAMKFNLLHAFDQNPPPEVFP 433

Query: 447 FKTYDIFNPPPNPEATVSDAIYRLDFNSTVDIILQNANMMRPNNSETHPWHLHGHDFWVL 506
            + YDI  PP N +  + + +Y+      VD+ILQNANMM+ N SE HPWHLHGHDFWVL
Sbjct: 434 -EDYDIDTPPTNEKTKIGNGVYQFKIGEIVDVILQNANMMKENLSEIHPWHLHGHDFWVL 493

Query: 507 GYGEGKFDMYKDPSKYNLENPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDAHFFMG 566
           GYG+GKF   ++ S  NL+NP ++NTV + P+GWTA+RFVADNPGVWAFHCHI+ H  MG
Sbjct: 494 GYGDGKFTA-EEESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMG 553

Query: 567 MGVVFEEGIEKVGKLPTSIMGCG 589
           MGVVF EG+EKVG++PT  + CG
Sbjct: 554 MGVVFAEGVEKVGRIPTKALACG 569

[Close]

BLAST of Spg013029 vs. ExPASy Swiss-Prot
Match: P14133 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1)

HSP 1 Score: 620.2 bits (1598), Expect = 2.5e-176
Identity = 291/564 (51.60%), Postives = 377/564 (66.84%), Query Frame = 0


Query: 28  LLIFLCFFVFLLESQTAESRTLHYKWEVKSELKSPDCFKKLTMTINGRSPGPTIYAHQGD 87
           L++ + F   +  S+    +  HYKW+V+    SPDC + + M ING  PGPTI A+ GD
Sbjct: 18  LVLSIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIVMGINGEFPGPTIRANAGD 77

Query: 88  TVIVELKNSFGTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFKYQFVVDRPGTYL 147
            V+VEL N   TE V IHWHGI Q GTPW+DGT  ++QC I PGETF Y+FVVD+ GTY 
Sbjct: 78  IVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFTYRFVVDKAGTYF 137

Query: 148 YHAHYGMQREDGLYGSIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSHEHAAGLATPNSG 207
           YH H GMQR  GLYGS++V  PEG+SEPF YD + NL+L+DW+H+S H+   GL+  +  
Sbjct: 138 YHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDWWHQSVHKQEVGLS--SKP 197

Query: 208 FQWVGEPDSLLIQGRGRFNCSLLGSSSLASGTCNSSSPD-CVPYPLTVVPGKTYRLRVSS 267
            +W+GEP S+LI G+G+F+CS+    +     C  S  + C P+ L V P KTYR+R++S
Sbjct: 198 MRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPFILHVQPKKTYRIRIAS 257

Query: 268 LTSLSALSFQIEGHTMTVVEADGHYVEPFQVNTLFIYSGETYSVLIKADQDPSRNYWITT 327
            T+L++L+F I  H + VVEADG+YV+PF  + + IYSGE+YSVLI  DQ+P  NYW++ 
Sbjct: 258 TTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSVLITTDQNPLENYWVSI 317

Query: 328 NVVGRNRTTPPGLAIFNYYPNHQRKLPPTTPPAGPAWNDVAPRLAQSHAIKARQGFIHAP 387
            V  R   TPPGL + NY PN   KLP + PP  P W D       +  I A  G    P
Sbjct: 318 GVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRSKNFTFRIFAAMG-SPKP 377

Query: 388 PKSADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKPPTGYDF 447
           P   ++ + LLNTQN +NG  +W++NNVS  +P TPYL A+K  L   F+Q  PP  +  
Sbjct: 378 PVRYNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKMRLNTAFNQNPPPETFPL 437

Query: 448 KTYDIFNPPPNPEATVSDAIYRLDFNSTVDIILQNANMMRPNNSETHPWHLHGHDFWVLG 507
             YDI NPPPNPE T  + +Y+ +   TVD+ILQNANM+ PN SE HPWHLHGHDFWVLG
Sbjct: 438 -NYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSEIHPWHLHGHDFWVLG 497

Query: 508 YGEGKFDMYKDPSKYNLENPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDAHFFMGM 567
           YGEGKF   +D  K NL+NP ++NTV + P+GWTA+RFVADNPGVWAFHCHI+ H  MGM
Sbjct: 498 YGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGM 557

Query: 568 GVVFEEGIEKVGKLPTSIMGCGES 591
           GVVF EG+  VG +P   + CG +
Sbjct: 558 GVVFAEGVHMVGMIPPKALACGST 577

[Close]

BLAST of Spg013029 vs. ExPASy Swiss-Prot
Match: P37064 (L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1)

HSP 1 Score: 617.1 bits (1590), Expect = 2.1e-175
Identity = 285/544 (52.39%), Postives = 372/544 (68.38%), Query Frame = 0


Query: 46  SRTLHYKWEVKSELKSPDCFKKLTMTINGRSPGPTIYAHQGDTVIVELKNSFGTENVAIH 105
           S+  HYKWEV+    +P+C + + M ING+ PGPTI A+ GD+V+VEL N   TE V IH
Sbjct: 1   SQIRHYKWEVEYMFWAPNCNENIVMGINGQFPGPTIRANAGDSVVVELTNKLHTEGVVIH 60

Query: 106 WHGIRQIGTPWSDGTEGVTQCPILPGETFKYQFVVDRPGTYLYHAHYGMQREDGLYGSIV 165
           WHGI Q GTPW+DGT  ++QC I PGETF Y F VD PGT+ YH H GMQR  GLYGS++
Sbjct: 61  WHGILQRGTPWADGTASISQCAINPGETFFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLI 120

Query: 166 VWLPEGQSEPFSYDHDRNLILTDWYHKSSHEHAAGLATPNSGFQWVGEPDSLLIQGRGRF 225
           V  P+G+ EPF YD + NL+L+DW+H+S H+   GL+  +   +W+GEP ++L+ GRG+F
Sbjct: 121 VDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLS--SKPIRWIGEPQTILLNGRGQF 180

Query: 226 NCSLLGSSSLASGTCN-SSSPDCVPYPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTV 285
           +CS+          C    S  C PY   V P KTYR+R++S T+L+AL+F I  H + V
Sbjct: 181 DCSIAAKYDSNLEPCKLKGSESCAPYIFHVSPKKTYRIRIASTTALAALNFAIGNHQLLV 240

Query: 286 VEADGHYVEPFQVNTLFIYSGETYSVLIKADQDPSRNYWITTNVVGRNRTTPPGLAIFNY 345
           VEADG+YV+PF  + + IYSGE+YSVLI  DQ+PS NYW++     R+  TPPGL + NY
Sbjct: 241 VEADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWVSVGTRARHPNTPPGLTLLNY 300

Query: 346 YPNHQRKLPPTTPPAGPAWNDVAPRLAQSHAIKARQGFIHAPPKSADKVIVLLNTQNTVN 405
            PN   KLP + PP  PAW+D       ++ I A  G    PP   ++ I LLNTQN +N
Sbjct: 301 LPNSVSKLPTSPPPQTPAWDDFDRSKNFTYRITAAMG-SPKPPVKFNRRIFLLNTQNVIN 360

Query: 406 GRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKPPTGYDFKTYDIFNPPPNPEATVSD 465
           G  +W++N+VS  +P TPYL A+K NLLH F Q  PP  +  + YDI  PP N +  + +
Sbjct: 361 GYVKWAINDVSLALPPTPYLGAMKYNLLHAFDQNPPPEVFP-EDYDIDTPPTNEKTRIGN 420

Query: 466 AIYRLDFNSTVDIILQNANMMRPNNSETHPWHLHGHDFWVLGYGEGKFDMYKDPSKYNLE 525
            +Y+      VD+ILQNANMM+ N SETHPWHLHGHDFWVLGYG+GKF   ++ S  NL+
Sbjct: 421 GVYQFKIGEVVDVILQNANMMKENLSETHPWHLHGHDFWVLGYGDGKFSA-EEESSLNLK 480

Query: 526 NPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIEKVGKLPTSI 585
           NP ++NTV + P+GWTA+RFVADNPGVWAFHCHI+ H  MGMGVVF EG+EKVG++PT  
Sbjct: 481 NPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVEKVGRIPTKA 539

Query: 586 MGCG 589
           + CG
Sbjct: 541 LACG 539

[Close]

BLAST of Spg013029 vs. ExPASy Swiss-Prot
Match: M4DUF2 (L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1)

HSP 1 Score: 607.4 bits (1565), Expect = 1.7e-172
Identity = 284/556 (51.08%), Postives = 372/556 (66.91%), Query Frame = 0


Query: 41  SQTAESRTLHYKWEVKSELKSPDCFKKLTMTINGRSPGPTIYAHQGDTVIVELKNSFGTE 100
           + TA +    Y WEV+ +   PDC + + M +NG+ PGPTI+A  GDT++V L N   TE
Sbjct: 13  AHTASAAVREYAWEVEYKFGWPDCKEGMVMAVNGQFPGPTIHALAGDTIVVHLTNKLATE 72

Query: 101 NVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFKYQFVVDRPGTYLYHAHYGMQREDGL 160
            + IHWHGIRQ+G+PW+DG  GVTQC I PGETF Y F VD+PGT+ YH HYGMQR  GL
Sbjct: 73  GLVIHWHGIRQLGSPWADGAAGVTQCAISPGETFTYNFTVDKPGTHFYHGHYGMQRSAGL 132

Query: 161 YGSIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSHEHAAGLATPNSGFQWVGEPDSLLIQ 220
           YGS+++ + +G+ EP  YD + NL+L+DW+H+       GL+  +   +W+GE  S+LI 
Sbjct: 133 YGSLIIDVAKGKKEPLRYDGEFNLLLSDWWHEDVLSQEIGLS--SRPMRWIGEAQSILIN 192

Query: 221 GRGRFNCSLLGS-SSLASGTCNSSSPD-CVPYPLTVVPGKTYRLRVSSLTSLSALSFQIE 280
           GRG+FNCSL    SS +  TC     D C P  L V P KTYR+R++S T+L++L+F ++
Sbjct: 193 GRGQFNCSLAAQFSSTSLPTCTFKEGDQCAPQRLHVEPNKTYRIRLASSTALASLNFAVQ 252

Query: 281 GHTMTVVEADGHYVEPFQVNTLFIYSGETYSVLIKADQDPSRNYWITTNVVGRNRTTPPG 340
           GH + VVEADG+Y+ PF  + + IYSGETYSVL+  DQDPS+NY+IT  V GR   TPP 
Sbjct: 253 GHKLVVVEADGNYITPFTTDDIDIYSGETYSVLLTTDQDPSQNYYITAGVRGRKPNTPPA 312

Query: 341 LAIFNYYPNHQRKLPPTTPPAGPAWNDVAPRLAQSHAIKARQGFIHAPPKSADKVIVLLN 400
           L + NY      +LP + PP  P WND       S  I A  G   +PP++ D+ ++LLN
Sbjct: 313 LTVLNYVTAPSSQLPTSPPPETPRWNDFDRSKNFSKKIFAAMG-SPSPPETFDERLILLN 372

Query: 401 TQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKPPTGYDFKTYDIFNPPPNP 460
           TQN + G  +W++NNVS  +P TPYL ++K NL   F+++ PP  Y    YDI  PP N 
Sbjct: 373 TQNLIEGFTKWAINNVSLAVPGTPYLGSVKYNLRTGFNRSSPPKDYPV-DYDIMTPPRNR 432

Query: 461 EATVSDAIYRLDFNSTVDIILQNANMMRPNNSETHPWHLHGHDFWVLGYGEGKFDMYKDP 520
            A   +      FN TVD+ILQNAN +  N SE HPWHLHGHDFWVLGYGEGKF    D 
Sbjct: 433 NAKQGNVSCVFPFNVTVDVILQNANGLNANASEIHPWHLHGHDFWVLGYGEGKFKPGVDE 492

Query: 521 SKYNLENPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIEKVG 580
             YNL+NP ++NTV L+P+GWTALRFV DNPGVW FHCHI+ H  MGMGVVF EG+ ++G
Sbjct: 493 KTYNLKNPPLRNTVALYPYGWTALRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEGLNRIG 552

Query: 581 KLPTSIMGCGESKRFI 595
           K+P   +GCG +K+F+
Sbjct: 553 KVPDEALGCGLTKQFL 564

[Close]

BLAST of Spg013029 vs. ExPASy Swiss-Prot
Match: Q40588 (L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1)

HSP 1 Score: 595.1 bits (1533), Expect = 8.6e-169
Identity = 294/573 (51.31%), Postives = 374/573 (65.27%), Query Frame = 0


Query: 31  FLCFF---VFLLESQTAES----RTLHYKWEVKSELKSPDCFKKLTMTINGRSPGPTIYA 90
           FL FF   + LLE  ++ S    +T H+KW+V+    SPD  + + M ING+ PGPTI A
Sbjct: 6   FLFFFLLPLILLELSSSRSVMAAKTRHFKWDVEYIHWSPDGEESVVMGINGQFPGPTIRA 65

Query: 91  HQGDTVIVELKNSFGTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFKYQFVVDRP 150
             GDTV V L N   TE V IHWHGIRQIGTPW+DGT  ++QC I PGETF Y+F VD+ 
Sbjct: 66  KAGDTVAVHLTNKLHTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGETFLYRFKVDKA 125

Query: 151 GTYLYHAHYGMQREDGLYGSIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSHEHAAGLAT 210
           GTY YH HYGMQR  GLYGS++V + EG+ EPF YD + NL+L+DW+HK SHE    L+ 
Sbjct: 126 GTYFYHGHYGMQRSAGLYGSLIVEVGEGEKEPFHYDGEFNLLLSDWWHKGSHEQEVDLS- 185

Query: 211 PNSGFQWVGEPDSLLIQGRGRFNCSLLGSSSLASGTCNSSSPDC--------VPYPLTVV 270
            ++  +W+GEP +LL+ GRG++NC      SLA+       P C         P  L V 
Sbjct: 186 -SNPLRWIGEPQTLLLNGRGQYNC------SLAARFSKPPLPQCKLRGGEQYAPQILRVR 245

Query: 271 PGKTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNTLFIYSGETYSVLIKAD 330
           P K YRLRV+S T+L +LS  I GH M VVEADG+YV+PF V  + IYSGE+YSVL K D
Sbjct: 246 PNKIYRLRVASTTALGSLSLAIGGHKMVVVEADGNYVQPFSVQDMDIYSGESYSVLFKTD 305

Query: 331 QDPSRNYWITTNVVGRNRTTPPGLAIFNYYPNHQRKLPPTTPPAGPAWNDVAPRLAQSHA 390
           QDP++NYWI+ NV GR   TP GL + NY PN   K P   PP  P WND     + S+ 
Sbjct: 306 QDPTKNYWISINVRGREPKTPQGLTLLNYLPNSASKFPTLPPPIAPLWNDYNHSKSFSNK 365

Query: 391 IKARQGFIHAPPKSADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTF 450
           I A  G    PP++  + I+LLNTQN ++G  +W++NNVS  +P   YL +++   ++ F
Sbjct: 366 IFALMGSPKPPPQN-HRRIILLNTQNKIDGYTKWAINNVSLVLPTQLYLGSIRYG-INAF 425

Query: 451 SQAKPPTGYDFKTYDIFNPPPNPEATVSDAIYRLDFNSTVDIILQNANMMRPNNSETHPW 510
               PP  +  K YD+    PN  +T  + +Y L FN+T+DIILQNAN +  + SE HPW
Sbjct: 426 DTKPPPDNFP-KDYDVLKQAPNSNSTYGNGVYMLKFNTTIDIILQNANALAKDVSEIHPW 485

Query: 511 HLHGHDFWVLGYGEGKFDMYKDPSKYNLENPIMKNTVPLHPFGWTALRFVADNPGVWAFH 570
           HLHGHDFWVLGYGEGKF   KD  K+NL+NP ++NT  + PFGWTALRFV DNPGVWAFH
Sbjct: 486 HLHGHDFWVLGYGEGKFS-EKDVKKFNLKNPPLRNTAVIFPFGWTALRFVTDNPGVWAFH 545

Query: 571 CHIDAHFFMGMGVVFEEGIEKVGKLPTSIMGCG 589
           CHI+ H  MGMGV+F EG+  V K+P   + CG
Sbjct: 546 CHIEPHLHMGMGVIFAEGVHLVKKIPKEALACG 566

[Close]

BLAST of Spg013029 vs. ExPASy TrEMBL
Match: A0A5A7SYW4 (L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002620 PE=3 SV=1)

HSP 1 Score: 1126.3 bits (2912), Expect = 0.0e+00
Identity = 530/579 (91.54%), Postives = 552/579 (95.34%), Query Frame = 0


Query: 18  MASLLQGRQILLIFLCFFVFLLESQTAESRTLHYKWEVKSELKSPDCFKKLTMTINGRSP 77
           MASLLQ +Q  L+ L  F+FLL+SQTAESRTL+YKWEVKSELKSPDCFKKLT+TINGRSP
Sbjct: 1   MASLLQRKQYFLLLLSSFLFLLQSQTAESRTLNYKWEVKSELKSPDCFKKLTITINGRSP 60

Query: 78  GPTIYAHQGDTVIVELKNSFGTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFKYQ 137
           GPTIYAHQGDTVIVELKNS  TENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETF+YQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSLHTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120

Query: 138 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSHEH 197
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVW+PEGQ+EPFSYDHDRNLILTDWYHKSS+EH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDHDRNLILTDWYHKSSNEH 180

Query: 198 AAGLATPNSGFQWVGEPDSLLIQGRGRFNCSLLGSSSLASGTCNSSSPDCVPYPLTVVPG 257
           AAGLATP SGF WVGEPDSLLIQGRGRFNCS+LGSS   SGTCNSSS +CV YPLTVVPG
Sbjct: 181 AAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSS---SGTCNSSSTECVLYPLTVVPG 240

Query: 258 KTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNTLFIYSGETYSVLIKADQD 317
           KTYRLRVSSLTSLSALSFQIEGH+MTVVEADGHYVEPFQVN LFIYSGETYSVLIKADQD
Sbjct: 241 KTYRLRVSSLTSLSALSFQIEGHSMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQD 300

Query: 318 PSRNYWITTNVVGRNRTTPPGLAIFNYYPNHQRKLPPTTPPAGPAWNDVAPRLAQSHAIK 377
           PSRNYWITTNVV R+RTTPPGLAIFNYYPNHQ K PPTTPPA PAWNDV PRLAQS AIK
Sbjct: 301 PSRNYWITTNVVSRSRTTPPGLAIFNYYPNHQSKRPPTTPPAPPAWNDVPPRLAQSQAIK 360

Query: 378 ARQGFIHAPPKSADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQ 437
           ARQGFIHAPPK+ADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALK+NLLHTFSQ
Sbjct: 361 ARQGFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQ 420

Query: 438 AKPPTGYDFKTYDIFNPPPNPEATVSDAIYRLDFNSTVDIILQNANMMRPNNSETHPWHL 497
            KPPTGYDFK YDIFNP PNPEATVSDAI+RLDFNSTVD+ILQNANMM PNNSETHPWHL
Sbjct: 421 EKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDLILQNANMMSPNNSETHPWHL 480

Query: 498 HGHDFWVLGYGEGKFDMYKDPSKYNLENPIMKNTVPLHPFGWTALRFVADNPGVWAFHCH 557
           HGHDFWVLGYGEGKFD+Y DPSKYNLENPIMKNTVPLHP+GWTALRFVADNPGVWAFHCH
Sbjct: 481 HGHDFWVLGYGEGKFDIYNDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCH 540

Query: 558 IDAHFFMGMGVVFEEGIEKVGKLPTSIMGCGESKRFIRP 597
           IDAHFFMGMGVVFEEGIE+VGKLPTSIMGCGESKRF+RP
Sbjct: 541 IDAHFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 576

[Close]

BLAST of Spg013029 vs. ExPASy TrEMBL
Match: A0A1S3B151 (L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103484994 PE=3 SV=1)

HSP 1 Score: 1126.3 bits (2912), Expect = 0.0e+00
Identity = 530/579 (91.54%), Postives = 552/579 (95.34%), Query Frame = 0


Query: 18  MASLLQGRQILLIFLCFFVFLLESQTAESRTLHYKWEVKSELKSPDCFKKLTMTINGRSP 77
           MASLLQ +Q  L+ L  F+FLL+SQTAESRTL+YKWEVKSELKSPDCFKKLT+TINGRSP
Sbjct: 1   MASLLQRKQYFLLLLSSFLFLLQSQTAESRTLNYKWEVKSELKSPDCFKKLTITINGRSP 60

Query: 78  GPTIYAHQGDTVIVELKNSFGTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFKYQ 137
           GPTIYAHQGDTVIVELKNS  TENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETF+YQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSLHTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120

Query: 138 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSHEH 197
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVW+PEGQ+EPFSYDHDRNLILTDWYHKSS+EH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDHDRNLILTDWYHKSSNEH 180

Query: 198 AAGLATPNSGFQWVGEPDSLLIQGRGRFNCSLLGSSSLASGTCNSSSPDCVPYPLTVVPG 257
           AAGLATP SGF WVGEPDSLLIQGRGRFNCS+LGSS   SGTCNSSS +CV YPLTVVPG
Sbjct: 181 AAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSS---SGTCNSSSTECVLYPLTVVPG 240

Query: 258 KTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNTLFIYSGETYSVLIKADQD 317
           KTYRLRVSSLTSLSALSFQIEGH+MTVVEADGHYVEPFQVN LFIYSGETYSVLIKADQD
Sbjct: 241 KTYRLRVSSLTSLSALSFQIEGHSMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQD 300

Query: 318 PSRNYWITTNVVGRNRTTPPGLAIFNYYPNHQRKLPPTTPPAGPAWNDVAPRLAQSHAIK 377
           PSRNYWITTNVV R+RTTPPGLAIFNYYPNHQ K PPTTPPA PAWNDV PRLAQS AIK
Sbjct: 301 PSRNYWITTNVVSRSRTTPPGLAIFNYYPNHQSKRPPTTPPAPPAWNDVPPRLAQSQAIK 360

Query: 378 ARQGFIHAPPKSADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQ 437
           ARQGFIHAPPK+ADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALK+NLLHTFSQ
Sbjct: 361 ARQGFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQ 420

Query: 438 AKPPTGYDFKTYDIFNPPPNPEATVSDAIYRLDFNSTVDIILQNANMMRPNNSETHPWHL 497
            KPPTGYDFK YDIFNP PNPEATVSDAI+RLDFNSTVD+ILQNANMM PNNSETHPWHL
Sbjct: 421 EKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDLILQNANMMSPNNSETHPWHL 480

Query: 498 HGHDFWVLGYGEGKFDMYKDPSKYNLENPIMKNTVPLHPFGWTALRFVADNPGVWAFHCH 557
           HGHDFWVLGYGEGKFD+Y DPSKYNLENPIMKNTVPLHP+GWTALRFVADNPGVWAFHCH
Sbjct: 481 HGHDFWVLGYGEGKFDIYNDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCH 540

Query: 558 IDAHFFMGMGVVFEEGIEKVGKLPTSIMGCGESKRFIRP 597
           IDAHFFMGMGVVFEEGIE+VGKLPTSIMGCGESKRF+RP
Sbjct: 541 IDAHFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 576

[Close]

BLAST of Spg013029 vs. ExPASy TrEMBL
Match: A0A6J1KU77 (L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=LOC111497239 PE=3 SV=1)

HSP 1 Score: 1125.9 bits (2911), Expect = 0.0e+00
Identity = 532/579 (91.88%), Postives = 548/579 (94.65%), Query Frame = 0


Query: 18  MASLLQGRQILLIFLCFFVFLLESQTAESRTLHYKWEVKSELKSPDCFKKLTMTINGRSP 77
           M SLLQ RQ LLI LCFFVFL ES+TAESRTL+Y+WEVKSELKSPDCFKKLT+TINGRSP
Sbjct: 1   MGSLLQRRQYLLILLCFFVFLWESRTAESRTLYYRWEVKSELKSPDCFKKLTLTINGRSP 60

Query: 78  GPTIYAHQGDTVIVELKNSFGTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFKYQ 137
           GPTIYAHQGDTVIVELKNSF TENVAIHWHGIRQIGTPWSDGTEGVTQCP+LPGETFKYQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQ 120

Query: 138 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSHEH 197
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQ+EPF+YDHDRNLILTDWYHKSSHEH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEH 180

Query: 198 AAGLATPNSGFQWVGEPDSLLIQGRGRFNCSLLGSSSLASGTCNSSSPDCVPYPLTVVPG 257
           +AGLA P S F WVGEPDSLLIQGRGRFNCSL      AS TCNSSSPDCV YP+TVVPG
Sbjct: 181 SAGLAAPGSAFNWVGEPDSLLIQGRGRFNCSL-----GASATCNSSSPDCVLYPITVVPG 240

Query: 258 KTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNTLFIYSGETYSVLIKADQD 317
           +TYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQV+ LFIYSGETYSVLIKADQ+
Sbjct: 241 RTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQN 300

Query: 318 PSRNYWITTNVVGRNRTTPPGLAIFNYYPNHQRKLPPTTPPAGPAWNDVAPRLAQSHAIK 377
           PSRNYWITTNVV RN TTP GLAIFNYYPNHQRK PPTTPPAGPAWN VAPRLAQSHAIK
Sbjct: 301 PSRNYWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIK 360

Query: 378 ARQGFIHAPPKSADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQ 437
           ARQGFIH PPK+ADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQ
Sbjct: 361 ARQGFIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQ 420

Query: 438 AKPPTGYDFKTYDIFNPPPNPEATVSDAIYRLDFNSTVDIILQNANMMRPNNSETHPWHL 497
           AKPPTGYDFK Y+IF+PPPNPEATVSDAIYRLDFNSTVDIILQNANMM  N SETHPWHL
Sbjct: 421 AKPPTGYDFKNYNIFDPPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHL 480

Query: 498 HGHDFWVLGYGEGKFDMYKDPSKYNLENPIMKNTVPLHPFGWTALRFVADNPGVWAFHCH 557
           HGHDFWVLGYGEGKFDM+KDPSKYNL NPIMKNTVPLHPFGWTALRFVADNPGVWAFHCH
Sbjct: 481 HGHDFWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCH 540

Query: 558 IDAHFFMGMGVVFEEGIEKVGKLPTSIMGCGESKRFIRP 597
           IDAHFFMGMGVVFEEGIEKV KLPTSIMGCGESKRFIRP
Sbjct: 541 IDAHFFMGMGVVFEEGIEKVQKLPTSIMGCGESKRFIRP 574

[Close]

BLAST of Spg013029 vs. ExPASy TrEMBL
Match: A0A0A0KK62 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=Csa_6G500640 PE=3 SV=1)

HSP 1 Score: 1124.4 bits (2907), Expect = 0.0e+00
Identity = 530/579 (91.54%), Postives = 549/579 (94.82%), Query Frame = 0


Query: 18  MASLLQGRQILLIFLCFFVFLLESQTAESRTLHYKWEVKSELKSPDCFKKLTMTINGRSP 77
           MAS LQ  + LLI L F  FLL+SQTAESRTL+YKWEVKSELKSPDCFKK+T+TINGRSP
Sbjct: 1   MASPLQSTRYLLILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSP 60

Query: 78  GPTIYAHQGDTVIVELKNSFGTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFKYQ 137
           GPTIYAHQGDTVIVELKNS  TENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETF+YQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120

Query: 138 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSHEH 197
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVW+PEGQ+EPFSYD DRNLILTDWYHKSSHEH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEH 180

Query: 198 AAGLATPNSGFQWVGEPDSLLIQGRGRFNCSLLGSSSLASGTCNSSSPDCVPYPLTVVPG 257
           A GLATP SGF WVGEPDSLLIQGRGRFNCS+LGSS   SGTCNSSS +CV +PLTVVPG
Sbjct: 181 ATGLATPGSGFNWVGEPDSLLIQGRGRFNCSILGSS---SGTCNSSSTECVLHPLTVVPG 240

Query: 258 KTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNTLFIYSGETYSVLIKADQD 317
           KTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVN LFIYSGETYSVLIKADQD
Sbjct: 241 KTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQD 300

Query: 318 PSRNYWITTNVVGRNRTTPPGLAIFNYYPNHQRKLPPTTPPAGPAWNDVAPRLAQSHAIK 377
           PSRNYWITTNVV R+RTTPPGLAIFNYYPNHQ +LPPTTPPA PAWNDV PRLAQS AIK
Sbjct: 301 PSRNYWITTNVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIK 360

Query: 378 ARQGFIHAPPKSADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQ 437
           ARQGFIH PPK+ADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALK+NLLHTFSQ
Sbjct: 361 ARQGFIHTPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQ 420

Query: 438 AKPPTGYDFKTYDIFNPPPNPEATVSDAIYRLDFNSTVDIILQNANMMRPNNSETHPWHL 497
            KPPTGYDFK YDIFNP PNPEATVSDAIYRLDFNSTVDIILQNANMM PNNSETHPWHL
Sbjct: 421 EKPPTGYDFKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHL 480

Query: 498 HGHDFWVLGYGEGKFDMYKDPSKYNLENPIMKNTVPLHPFGWTALRFVADNPGVWAFHCH 557
           HGHDFWVLGYGEGKFD+YKDPSKYNLENPIMKNTVPLHP+GWTALRFVADNPGVWAFHCH
Sbjct: 481 HGHDFWVLGYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCH 540

Query: 558 IDAHFFMGMGVVFEEGIEKVGKLPTSIMGCGESKRFIRP 597
           IDAHFFMGMGVVFEEGIE+VGKLPTSIMGCGESKRF+RP
Sbjct: 541 IDAHFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 576

[Close]

BLAST of Spg013029 vs. ExPASy TrEMBL
Match: A0A6J1HE32 (L-ascorbate oxidase OS=Cucurbita moschata OX=3662 GN=LOC111463148 PE=3 SV=1)

HSP 1 Score: 1124.0 bits (2906), Expect = 0.0e+00
Identity = 532/579 (91.88%), Postives = 547/579 (94.47%), Query Frame = 0


Query: 18  MASLLQGRQILLIFLCFFVFLLESQTAESRTLHYKWEVKSELKSPDCFKKLTMTINGRSP 77
           M SLLQ RQ LLI LCFFVFL ES+TAESRTL+YKWEVKSELKSPDCFKKLT+TINGRSP
Sbjct: 1   MGSLLQSRQNLLILLCFFVFLRESRTAESRTLYYKWEVKSELKSPDCFKKLTLTINGRSP 60

Query: 78  GPTIYAHQGDTVIVELKNSFGTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFKYQ 137
           GPTIYAHQGDTVIVELKNSF TENVAIHWHGIRQIGTPWSDGTEGVTQCP+LPGETFKYQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQ 120

Query: 138 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSHEH 197
           FVVDRPGTYLYHAHYGMQREDGL+GSIVVWLPEGQ+EPF+YDHDRNLILTDWYHKSSHEH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLFGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEH 180

Query: 198 AAGLATPNSGFQWVGEPDSLLIQGRGRFNCSLLGSSSLASGTCNSSSPDCVPYPLTVVPG 257
           +AGLA   S F WVGEPDSLLIQGRGRFNCS LG    AS TCNSSSPDCV YP+TVVPG
Sbjct: 181 SAGLAAAGSAFTWVGEPDSLLIQGRGRFNCSRLG----ASATCNSSSPDCVLYPITVVPG 240

Query: 258 KTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNTLFIYSGETYSVLIKADQD 317
           +TYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQV+ LFIYSGETYSVLIKADQ+
Sbjct: 241 RTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQN 300

Query: 318 PSRNYWITTNVVGRNRTTPPGLAIFNYYPNHQRKLPPTTPPAGPAWNDVAPRLAQSHAIK 377
           PSRNYWITTNVV RN TTP GLAIFNYYPNHQRK PPTTPPAGPAWN VAPRLAQSHAIK
Sbjct: 301 PSRNYWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIK 360

Query: 378 ARQGFIHAPPKSADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQ 437
           ARQGFIH PPK+ADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQ
Sbjct: 361 ARQGFIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQ 420

Query: 438 AKPPTGYDFKTYDIFNPPPNPEATVSDAIYRLDFNSTVDIILQNANMMRPNNSETHPWHL 497
           AKPPTGYDFK Y+IF PPPNPEATVSDAIYRLDFNSTVDIILQNANMM  N SETHPWHL
Sbjct: 421 AKPPTGYDFKNYNIFVPPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHL 480

Query: 498 HGHDFWVLGYGEGKFDMYKDPSKYNLENPIMKNTVPLHPFGWTALRFVADNPGVWAFHCH 557
           HGHDFWVLGYGEGKFDM+KDPSKYNL NPIMKNTVPLHPFGWTALRFVADNPGVWAFHCH
Sbjct: 481 HGHDFWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCH 540

Query: 558 IDAHFFMGMGVVFEEGIEKVGKLPTSIMGCGESKRFIRP 597
           IDAHFFMGMGVVFEEGIEKV KLPTSIMGCGESKRFIRP
Sbjct: 541 IDAHFFMGMGVVFEEGIEKVQKLPTSIMGCGESKRFIRP 575

[Close]

BLAST of Spg013029 vs. TAIR 10
Match: AT4G39830.1 (Cupredoxin superfamily protein )

HSP 1 Score: 825.1 bits (2130), Expect = 3.6e-239
Identity = 389/569 (68.37%), Postives = 448/569 (78.73%), Query Frame = 0


Query: 29  LIFLCFFVFLLESQTAESRTLHYKWEVKSELKSPDCFKKLTMTINGRSPGPTIYAHQGDT 88
           L+ LCF      S   + +   +KWEVK E KSPDCF+KL +TING+ PGPTI A QGDT
Sbjct: 16  LMVLCFIALFFSSVLCQGKIRRFKWEVKYEFKSPDCFEKLVITINGKFPGPTIKAQQGDT 75

Query: 89  VIVELKNSFGTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFKYQFVVDRPGTYLY 148
           ++VELKNSF TENVA+HWHGIRQIGTPW DG EGVTQCPILPGE F YQFVVDRPGTY+Y
Sbjct: 76  IVVELKNSFMTENVAVHWHGIRQIGTPWFDGVEGVTQCPILPGEVFIYQFVVDRPGTYMY 135

Query: 149 HAHYGMQREDGLYGSIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSHEHAAGLATPNSGF 208
           H+HYGMQRE GL G I V  P  + EPF+YD+DRN +LTDWYHKS  E A GLA  +  F
Sbjct: 136 HSHYGMQRESGLIGMIQVSPPATEPEPFTYDYDRNFLLTDWYHKSMSEKATGLA--SIPF 195

Query: 209 QWVGEPDSLLIQGRGRFNCS--LLGSSSLASGTCNSSSPDCVPYPLTVVPGKTYRLRVSS 268
           +WVGEP SL+IQGRGRFNCS  L    SL SG CN S+ DC  + LTV+PGKTYRLR+ S
Sbjct: 196 KWVGEPQSLMIQGRGRFNCSNNLTTPPSLVSGVCNVSNADCSRFILTVIPGKTYRLRIGS 255

Query: 269 LTSLSALSFQIEGHTMTVVEADGHYVEPFQVNTLFIYSGETYSVLIKADQDPSRNYWITT 328
           LT+LSALSFQIEGH +TVVEADGHYVEPF V  LF+YSGETYSVL+KADQ+P RNYWIT+
Sbjct: 256 LTALSALSFQIEGHNLTVVEADGHYVEPFTVKNLFVYSGETYSVLLKADQNPRRNYWITS 315

Query: 329 NVVGRNRTTPPGLAIFNYYPNHQRKLPPTTPPAG--PAWNDVAPRLAQSHAIKARQGFIH 388
           ++V R  TTPP  A+ NYYPNH R+ PPT+  +   P WND   RLAQS AIKAR+GFIH
Sbjct: 316 SIVSRPATTPPATAVLNYYPNHPRRRPPTSESSNIVPEWNDTRSRLAQSLAIKARRGFIH 375

Query: 389 APPKSADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFS-QAKPPTG 448
           A P+++DKVIVLLNTQN VNG RRWS+NNVS++ P TPYLIALK NL + F  +   P  
Sbjct: 376 ALPENSDKVIVLLNTQNEVNGYRRWSVNNVSYHHPKTPYLIALKQNLTNAFDWRFTAPEN 435

Query: 449 YDFKTYDIFNPPPNPEATVSDAIYRLDFNSTVDIILQNANMMRPNNSETHPWHLHGHDFW 508
           YD + YDIF  P N  AT SD IYRL FNSTVD+ILQNAN M  NNSETHPWHLHGHDFW
Sbjct: 436 YDSRNYDIFAKPLNANATTSDGIYRLRFNSTVDVILQNANTMNANNSETHPWHLHGHDFW 495

Query: 509 VLGYGEGKFDMYKDPSKYNLENPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDAHFF 568
           VLGYGEGKF+  +DP +YN  +PI KNTV + PFGWTALRF ADNPGVW+FHCHI++HFF
Sbjct: 496 VLGYGEGKFNESEDPKRYNRVDPIKKNTVAVQPFGWTALRFRADNPGVWSFHCHIESHFF 555

Query: 569 MGMGVVFEEGIEKVGKLPTSIMGCGESKR 593
           MGMG+VFE GI+KV  LP+SIMGCG++KR
Sbjct: 556 MGMGIVFESGIDKVSSLPSSIMGCGQTKR 582

[Close]

BLAST of Spg013029 vs. TAIR 10
Match: AT5G21105.1 (Plant L-ascorbate oxidase )

HSP 1 Score: 594.7 bits (1532), Expect = 8.0e-170
Identity = 280/557 (50.27%), Postives = 369/557 (66.25%), Query Frame = 0


Query: 41  SQTAESRTLHYKWEVKSELKSPDCFKKLTMTINGRSPGPTIYAHQGDTVIVELKNSFGTE 100
           + TA +    Y WEV+ +  SPDC +   MT+NG  PGPTI A  GDT++V L N   TE
Sbjct: 30  THTASAAVREYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAGDTIVVNLTNKLTTE 89

Query: 101 NVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFKYQFVVDRPGTYLYHAHYGMQREDGL 160
            + IHWHGIRQ G+PW+DG  GVTQC I PGETF Y F V++PGT+ YH HYGMQR  GL
Sbjct: 90  GLVIHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTHFYHGHYGMQRSAGL 149

Query: 161 YGSIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSHEHAAGLATPNSGFQWVGEPDSLLIQ 220
           YGS++V + +G+SE   YD + NL+L+DW+H++      GL+  +   +W+GE  S+LI 
Sbjct: 150 YGSLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLS--SKPMRWIGEAQSILIN 209

Query: 221 GRGRFNCSLLG--SSSLASGTCNSSSPD-CVPYPLTVVPGKTYRLRVSSLTSLSALSFQI 280
           GRG+FNCSL    S++ +   C     D C P  L V P KTYR+R+SS T+L++L+  +
Sbjct: 210 GRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALASLNLAV 269

Query: 281 EGHTMTVVEADGHYVEPFQVNTLFIYSGETYSVLIKADQDPSRNYWITTNVVGRNRTTPP 340
           +GH + VVEADG+Y+ PF  + + IYSGE+YSVL+  DQDPS+NY+I+  V GR   T  
Sbjct: 270 QGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGRKPNTTQ 329

Query: 341 GLAIFNYYPNHQRKLPPTTPPAGPAWNDVAPRLAQSHAIKARQGFIHAPPKSADKVIVLL 400
            L I NY      KLP + PP  P W+D       S  I +  G   +PPK   K ++LL
Sbjct: 330 ALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMG-SPSPPKKYRKRLILL 389

Query: 401 NTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKPPTGYDFKTYDIFNPPPN 460
           NTQN ++G  +W++NNVS   P TPYL ++K NL   F++  PP  Y    YDI NPPP 
Sbjct: 390 NTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRM-DYDIMNPPPF 449

Query: 461 PEATVSDAIYRLDFNSTVDIILQNANMMRPNNSETHPWHLHGHDFWVLGYGEGKFDMYKD 520
           P  T  + IY   FN TVD+I+QNAN+++   SE HPWHLHGHDFWVLGYG+GKF    D
Sbjct: 450 PNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKFKPGID 509

Query: 521 PSKYNLENPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIEKV 580
              YNL+NP ++NT  L+P+GWTA+RFV DNPGVW FHCHI+ H  MGMGVVF EG+ ++
Sbjct: 510 EKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEGLNRI 569

Query: 581 GKLPTSIMGCGESKRFI 595
           GK+P   +GCG +K+F+
Sbjct: 570 GKVPDEALGCGLTKQFL 582

[Close]

BLAST of Spg013029 vs. TAIR 10
Match: AT5G21100.1 (Plant L-ascorbate oxidase )

HSP 1 Score: 568.5 bits (1464), Expect = 6.1e-162
Identity = 274/572 (47.90%), Postives = 365/572 (63.81%), Query Frame = 0


Query: 28  LLIFLCFFVFLLESQTAESRTLHYKWEVKSELKSPDCFKKLTMTINGRSPGPTIYAHQGD 87
           ++++    V ++   +A +  +   WEV+ +   PDC + + M ING+ PGPTI A  GD
Sbjct: 3   VIVWWLLTVVVVAFHSASAAVVESTWEVEYKYWWPDCKEGIVMAINGQFPGPTIDAVAGD 62

Query: 88  TVIVELKNSFGTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFKYQFVVDRPGTYL 147
           TVI+ + N   TE V IHWHGIRQ GTPW+DG  GVTQCPI PGETF Y+F+VD+ GT+ 
Sbjct: 63  TVIIHVVNKLSTEGVVIHWHGIRQKGTPWADGAAGVTQCPINPGETFTYKFIVDKAGTHF 122

Query: 148 YHAHYGMQREDGLYGSIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSHEHAAGLATPNSG 207
           YH HYGMQR  GLYG ++V  P+   E   YD + NL+L+DW+H+S   HA  LA  +  
Sbjct: 123 YHGHYGMQRSSGLYGMLIVRSPK---ERLIYDGEFNLLLSDWWHQSI--HAQELALSSRP 182

Query: 208 FQWVGEPDSLLIQGRGRFNCSLLG--SSSLASGTCNSSSPD-CVPYPLTVVPGKTYRLRV 267
            +W+GEP SLLI GRG+FNCS     +       C     D C P  L V P + YRLR+
Sbjct: 183 MRWIGEPQSLLINGRGQFNCSQAAYFNKGGEKDVCTFKENDQCAPQTLRVEPNRVYRLRI 242

Query: 268 SSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNTLFIYSGETYSVLIKADQDPSRNYWI 327
           +S T+L++L+  ++GH + VVEADG+YV PF VN + +YSGETYSVL+K +  PS+ YWI
Sbjct: 243 ASTTALASLNLAVQGHQLVVVEADGNYVAPFTVNDIDVYSGETYSVLLKTNALPSKKYWI 302

Query: 328 TTNVVGRNRTTPPGLAIFNYYPNHQRKLPPTTPPAGPAWNDVAPRLAQSHAIKARQGFIH 387
           +  V GR   TP  L + NY    + + P   PP  P WND     + S  I A +G+  
Sbjct: 303 SVGVRGREPKTPQALTVINYVDATESR-PSHPPPVTPIWNDTDRSKSFSKKIFAAKGYPK 362

Query: 388 APPKSADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKPPTGY 447
            P KS D+ ++LLNTQN      +WS+NNVS ++P TPYL +++  L   +    P    
Sbjct: 363 PPEKSHDQ-LILLNTQNLYEDYTKWSINNVSLSVPVTPYLGSIRYGLKSAYDLKSPAKKL 422

Query: 448 DFKTYDIFNPPPNPEATVSDAIYRLDFNSTVDIILQNANMMRPNNSETHPWHLHGHDFWV 507
               YDI  PPPNP  T    IY   F   VD+ILQNAN+++   SE HPWH+HGHDFWV
Sbjct: 423 IMDNYDIMKPPPNPNTTKGSGIYNFAFGIVVDVILQNANVLKGVISEIHPWHIHGHDFWV 482

Query: 508 LGYGEGKFDMYKDPSKYNLENPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDAHFFM 567
           LGYGEGKF    D   +NL+NP ++NTV L+PFGWTA+RFV DNPGVW FHCHI+ H  M
Sbjct: 483 LGYGEGKFKPGIDEKTFNLKNPPLRNTVVLYPFGWTAIRFVTDNPGVWFFHCHIEPHLHM 542

Query: 568 GMGVVFEEGIEKVGK--LPTSIMGCGESKRFI 595
           GMGVVF EG++++GK  +P   +GCG +++++
Sbjct: 543 GMGVVFVEGVDRIGKMEIPDEALGCGLTRKWL 567

[Close]

BLAST of Spg013029 vs. TAIR 10
Match: AT5G21105.3 (Plant L-ascorbate oxidase )

HSP 1 Score: 531.2 bits (1367), Expect = 1.1e-150
Identity = 255/513 (49.71%), Postives = 335/513 (65.30%), Query Frame = 0


Query: 41  SQTAESRTLHYKWEVKSELKSPDCFKKLTMTINGRSPGPTIYAHQGDTVIVELKNSFGTE 100
           + TA +    Y WEV+ +  SPDC +   MT+NG  PGPTI A  GDT++V L N   TE
Sbjct: 30  THTASAAVREYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAGDTIVVNLTNKLTTE 89

Query: 101 NVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFKYQFVVDRPGTYLYHAHYGMQREDGL 160
            + IHWHGIRQ G+PW+DG  GVTQC I PGETF Y F V++PGT+ YH HYGMQR  GL
Sbjct: 90  GLVIHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTHFYHGHYGMQRSAGL 149

Query: 161 YGSIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSHEHAAGLATPNSGFQWVGEPDSLLIQ 220
           YGS++V + +G+SE   YD + NL+L+DW+H++      GL+  +   +W+GE  S+LI 
Sbjct: 150 YGSLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLS--SKPMRWIGEAQSILIN 209

Query: 221 GRGRFNCSLLG--SSSLASGTCNSSSPD-CVPYPLTVVPGKTYRLRVSSLTSLSALSFQI 280
           GRG+FNCSL    S++ +   C     D C P  L V P KTYR+R+SS T+L++L+  +
Sbjct: 210 GRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALASLNLAV 269

Query: 281 EGHTMTVVEADGHYVEPFQVNTLFIYSGETYSVLIKADQDPSRNYWITTNVVGRNRTTPP 340
           +GH + VVEADG+Y+ PF  + + IYSGE+YSVL+  DQDPS+NY+I+  V GR   T  
Sbjct: 270 QGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGRKPNTTQ 329

Query: 341 GLAIFNYYPNHQRKLPPTTPPAGPAWNDVAPRLAQSHAIKARQGFIHAPPKSADKVIVLL 400
            L I NY      KLP + PP  P W+D       S  I +  G   +PPK   K ++LL
Sbjct: 330 ALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMG-SPSPPKKYRKRLILL 389

Query: 401 NTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKPPTGYDFKTYDIFNPPPN 460
           NTQN ++G  +W++NNVS   P TPYL ++K NL   F++  PP  Y    YDI NPPP 
Sbjct: 390 NTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRM-DYDIMNPPPF 449

Query: 461 PEATVSDAIYRLDFNSTVDIILQNANMMRPNNSETHPWHLHGHDFWVLGYGEGKFDMYKD 520
           P  T  + IY   FN TVD+I+QNAN+++   SE HPWHLHGHDFWVLGYG+GKF    D
Sbjct: 450 PNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKFKPGID 509

Query: 521 PSKYNLENPIMKNTVPLHPFGWTALRFVADNPG 551
              YNL+NP ++NT  L+P+GWTA+RFV DNPG
Sbjct: 510 EKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPG 538

[Close]

BLAST of Spg013029 vs. TAIR 10
Match: AT5G21105.2 (Plant L-ascorbate oxidase )

HSP 1 Score: 394.4 bits (1012), Expect = 1.6e-109
Identity = 192/382 (50.26%), Postives = 252/382 (65.97%), Query Frame = 0


Query: 216 SLLIQGRGRFNCSLLG--SSSLASGTCNSSSPD-CVPYPLTVVPGKTYRLRVSSLTSLSA 275
           S+LI GRG+FNCSL    S++ +   C     D C P  L V P KTYR+R+SS T+L++
Sbjct: 12  SILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALAS 71

Query: 276 LSFQIEGHTMTVVEADGHYVEPFQVNTLFIYSGETYSVLIKADQDPSRNYWITTNVVGRN 335
           L+  ++GH + VVEADG+Y+ PF  + + IYSGE+YSVL+  DQDPS+NY+I+  V GR 
Sbjct: 72  LNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGRK 131

Query: 336 RTTPPGLAIFNYYPNHQRKLPPTTPPAGPAWNDVAPRLAQSHAIKARQGFIHAPPKSADK 395
             T   L I NY      KLP + PP  P W+D       S  I +  G   +PPK   K
Sbjct: 132 PNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMG-SPSPPKKYRK 191

Query: 396 VIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKPPTGYDFKTYDIF 455
            ++LLNTQN ++G  +W++NNVS   P TPYL ++K NL   F++  PP  Y    YDI 
Sbjct: 192 RLILLNTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRM-DYDIM 251

Query: 456 NPPPNPEATVSDAIYRLDFNSTVDIILQNANMMRPNNSETHPWHLHGHDFWVLGYGEGKF 515
           NPPP P  T  + IY   FN TVD+I+QNAN+++   SE HPWHLHGHDFWVLGYG+GKF
Sbjct: 252 NPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKF 311

Query: 516 DMYKDPSKYNLENPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEE 575
               D   YNL+NP ++NT  L+P+GWTA+RFV DNPGVW FHCHI+ H  MGMGVVF E
Sbjct: 312 KPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFAE 371

Query: 576 GIEKVGKLPTSIMGCGESKRFI 595
           G+ ++GK+P   +GCG +K+F+
Sbjct: 372 GLNRIGKVPDEALGCGLTKQFL 391

The following BLAST results are available for this feature:

BLAST of Spg013029 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038881120.1	0.0e+00	91.78	L-ascorbate oxidase [Benincasa hispida]	[more]
KAG6594863.1	0.0e+00	92.23	L-ascorbate oxidase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026826...	[more]
XP_008440636.1	0.0e+00	91.54	PREDICTED: L-ascorbate oxidase [Cucumis melo] >KAA0036280.1 L-ascorbate oxidase ...	[more]
XP_023003749.1	0.0e+00	91.88	L-ascorbate oxidase [Cucurbita maxima]	[more]
XP_004143482.1	0.0e+00	91.54	L-ascorbate oxidase [Cucumis sativus] >KGN48772.1 hypothetical protein Csa_00307...	[more]

BLAST of Spg013029 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P24792	1.1e-176	51.87	L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2	[more]
P14133	2.5e-176	51.60	L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1	[more]
P37064	2.1e-175	52.39	L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1	[more]
M4DUF2	1.7e-172	51.08	L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1	[more]
Q40588	8.6e-169	51.31	L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1	[more]

BLAST of Spg013029 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7SYW4	0.0e+00	91.54	L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G...	[more]
A0A1S3B151	0.0e+00	91.54	L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103484994 PE=3 SV=1	[more]
A0A6J1KU77	0.0e+00	91.88	L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=LOC111497239 PE=3 SV=1	[more]
A0A0A0KK62	0.0e+00	91.54	L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=Csa_6G500640 PE=3 SV=1	[more]
A0A6J1HE32	0.0e+00	91.88	L-ascorbate oxidase OS=Cucurbita moschata OX=3662 GN=LOC111463148 PE=3 SV=1	[more]

BLAST of Spg013029 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G39830.1	3.6e-239	68.37	Cupredoxin superfamily protein	[more]
AT5G21105.1	8.0e-170	50.27	Plant L-ascorbate oxidase	[more]
AT5G21100.1	6.1e-162	47.90	Plant L-ascorbate oxidase	[more]
AT5G21105.3	1.1e-150	49.71	Plant L-ascorbate oxidase	[more]
AT5G21105.2	1.6e-109	50.26	Plant L-ascorbate oxidase	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 384..594 e-value: 3.5E-70 score: 238.0
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 37..169 e-value: 8.6E-47 score: 161.1
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 176..382 e-value: 2.2E-63 score: 216.0
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 387..588
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 39..195
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 175..368
IPR011707	Multicopper oxidase, N-termianl	PFAM	PF07732	Cu-oxidase_3	coord: 58..167 e-value: 4.4E-38 score: 130.0
IPR001117	Multicopper oxidase, type 1	PFAM	PF00394	Cu-oxidase	coord: 184..346 e-value: 1.0E-47 score: 162.2
IPR011706	Multicopper oxidase, C-terminal	PFAM	PF07731	Cu-oxidase_2	coord: 457..573 e-value: 2.7E-37 score: 127.6
IPR017760	L-ascorbate oxidase, plants	TIGRFAM	TIGR03388	TIGR03388	coord: 50..589 e-value: 1.6E-284 score: 942.5
IPR045087	Multicopper oxidase	PANTHER	PTHR11709	MULTI-COPPER OXIDASE	coord: 31..590
None	No IPR available	PANTHER	PTHR11709:SF218	OS09G0365900 PROTEIN	coord: 31..590
IPR033138	Multicopper oxidases, conserved site	PROSITE	PS00079	MULTICOPPER_OXIDASE1	coord: 550..570
IPR002355	Multicopper oxidase, copper-binding site	PROSITE	PS00080	MULTICOPPER_OXIDASE2	coord: 555..566
IPR034267	Ascorbate oxidase, third cupredoxin domain	CDD	cd13893	CuRO_3_AAO	coord: 390..581 e-value: 1.05678E-84 score: 259.274

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg013029.1	Spg013029.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:1900367	positive regulation of defense response to insect
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005507	copper ion binding
molecular_function	GO:0008447	L-ascorbate oxidase activity
molecular_function	GO:0016491	oxidoreductase activity