Spg013028 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg013028
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Eukaryotic aspartyl protease family protein
Location	scaffold1: 11466483 .. 11467865 (-)
RNA-Seq Expression	Spg013028
Synteny	Spg013028

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTTTCTCCCCATTCCCTTTCTCTTTTCCATCTTTCTCCTTCTTTCCACTTCATCTTCTTCCTCCACTTCCCCCATCACACTCCCCCTCACCACCTTCCCTTCAGCTCCAGTTACAGATCCATGGCAGAACATCAATTTCCTCGTCTCTGCTTCACTCACCAGAGCTCATCACCTCAAGAGGCCGCGAACGAGTCCAAACAGTTCCATCGAGAAAGTCTCTCTGTTCTCTCGTAGCTATGGAGCTTATTCGATCTCCCTCAGCTTCGGAACTCCACCGCAGAATTTGTCGTTCGTCTTCGATACGGGAAGCAGCCTCGTCTGGTTCCCCTGCACCGCCGGTTATCTCTGTTCCAATTGTTCGTTTCCGAACTCGGATACTAAGACGATTGCGAAATTTATCCCCAAATTATCTTCCTCTGCGAAGATCGTCGGTTGCCGAAATCGAAAATGTGCTTGGATTTTTGGCCCTAATTTGAAGTCCAGATGTAGAAATTGTAACCCTAAATCTCGAAGGTGTTTCGATTCTTGTCCTGGTTATGGAATTCAGTACGGCTCCGGCGCAACCGCTGGATTTCTCCTCTCTGAAACTCTGGATTTTCCGGAGAAACGAGTGACTGATTTTCTCGTCGGTTGTTCCGTCTTGTCCGTTCATCTACCCGCCGGCATTGCCGGATTCGGCCGCGGCCCTGAATCGTTGCCCTCGCAAATGAGACTGAAACGATTCTCCTATTGCCTGGTTTCTCGCCGGTTCGACGACTCGCCGGTGAGTAGCCCTCTCGTACTGGACTCCGGTTCGGAATCCGGCGAATCGAAGACGAACCGTCTCATTTACGCACCGTTCCGAGAGAATCCGTCGGGATCCAACGCCGCATTTCGAGAATTCTATTACCTTTCTCTTCGGAGAATCCTCATCGGTGGAAAGCCAGTGAAGTTTCCGTACAAGTATCTCGTGCCGGACTCCGCCGGAAACGGCGGTGCGATCATCGATTCCGGTTCGACGTTTACATTTCTCGATAAGCCGATTTTCGAAGCCGTAGCGGAGGAGTTGGAGAAGCAACTGGTGAAATATCCCCGAGCGACCGGCGTTGAAGCCCAGTCCGGTTTGAGGCCGTGCTTCGATATATCCAAGGAGGAGAAGGTGGAGTTTCCGGAACTGGTTTTGAAGTTTAAAGGCGGAGCGAAGCTGGCTTTGCCGCCGGCGAATTACTTGGCGTTGGTGACGGATTCCGGCGTGGTTTGCTTGACGATGATGACGGATGAGGCCGGAGGCGGCGGTGGGCCGGCGATTATAATGGGGGCGTTTCAGCAGCAGAATATTTTGGTGGAGTACGATTTGGCGAAGGAGAGAATCGGATTTCGAAAGCAGAGATGCACGTGA

mRNA sequence

ATGGAGTTTCTCCCCATTCCCTTTCTCTTTTCCATCTTTCTCCTTCTTTCCACTTCATCTTCTTCCTCCACTTCCCCCATCACACTCCCCCTCACCACCTTCCCTTCAGCTCCAGTTACAGATCCATGGCAGAACATCAATTTCCTCGTCTCTGCTTCACTCACCAGAGCTCATCACCTCAAGAGGCCGCGAACGAGTCCAAACAGTTCCATCGAGAAAGTCTCTCTGTTCTCTCGTAGCTATGGAGCTTATTCGATCTCCCTCAGCTTCGGAACTCCACCGCAGAATTTGTCGTTCGTCTTCGATACGGGAAGCAGCCTCGTCTGGTTCCCCTGCACCGCCGGTTATCTCTGTTCCAATTGTTCGTTTCCGAACTCGGATACTAAGACGATTGCGAAATTTATCCCCAAATTATCTTCCTCTGCGAAGATCGTCGGTTGCCGAAATCGAAAATGTGCTTGGATTTTTGGCCCTAATTTGAAGTCCAGATGTAGAAATTGTAACCCTAAATCTCGAAGGTGTTTCGATTCTTGTCCTGGTTATGGAATTCAGTACGGCTCCGGCGCAACCGCTGGATTTCTCCTCTCTGAAACTCTGGATTTTCCGGAGAAACGAGTGACTGATTTTCTCGTCGGTTGTTCCGTCTTGTCCGTTCATCTACCCGCCGGCATTGCCGGATTCGGCCGCGGCCCTGAATCGTTGCCCTCGCAAATGAGACTGAAACGATTCTCCTATTGCCTGGTTTCTCGCCGGTTCGACGACTCGCCGGTGAGTAGCCCTCTCGTACTGGACTCCGGTTCGGAATCCGGCGAATCGAAGACGAACCGTCTCATTTACGCACCGTTCCGAGAGAATCCGTCGGGATCCAACGCCGCATTTCGAGAATTCTATTACCTTTCTCTTCGGAGAATCCTCATCGGTGGAAAGCCAGTGAAGTTTCCGTACAAGTATCTCGTGCCGGACTCCGCCGGAAACGGCGGTGCGATCATCGATTCCGGTTCGACGTTTACATTTCTCGATAAGCCGATTTTCGAAGCCGTAGCGGAGGAGTTGGAGAAGCAACTGGTGAAATATCCCCGAGCGACCGGCGTTGAAGCCCAGTCCGGTTTGAGGCCGTGCTTCGATATATCCAAGGAGGAGAAGGTGGAGTTTCCGGAACTGGTTTTGAAGTTTAAAGGCGGAGCGAAGCTGGCTTTGCCGCCGGCGAATTACTTGGCGTTGGTGACGGATTCCGGCGTGGTTTGCTTGACGATGATGACGGATGAGGCCGGAGGCGGCGGTGGGCCGGCGATTATAATGGGGGCGTTTCAGCAGCAGAATATTTTGGTGGAGTACGATTTGGCGAAGGAGAGAATCGGATTTCGAAAGCAGAGATGCACGTGA

Coding sequence (CDS)

ATGGAGTTTCTCCCCATTCCCTTTCTCTTTTCCATCTTTCTCCTTCTTTCCACTTCATCTTCTTCCTCCACTTCCCCCATCACACTCCCCCTCACCACCTTCCCTTCAGCTCCAGTTACAGATCCATGGCAGAACATCAATTTCCTCGTCTCTGCTTCACTCACCAGAGCTCATCACCTCAAGAGGCCGCGAACGAGTCCAAACAGTTCCATCGAGAAAGTCTCTCTGTTCTCTCGTAGCTATGGAGCTTATTCGATCTCCCTCAGCTTCGGAACTCCACCGCAGAATTTGTCGTTCGTCTTCGATACGGGAAGCAGCCTCGTCTGGTTCCCCTGCACCGCCGGTTATCTCTGTTCCAATTGTTCGTTTCCGAACTCGGATACTAAGACGATTGCGAAATTTATCCCCAAATTATCTTCCTCTGCGAAGATCGTCGGTTGCCGAAATCGAAAATGTGCTTGGATTTTTGGCCCTAATTTGAAGTCCAGATGTAGAAATTGTAACCCTAAATCTCGAAGGTGTTTCGATTCTTGTCCTGGTTATGGAATTCAGTACGGCTCCGGCGCAACCGCTGGATTTCTCCTCTCTGAAACTCTGGATTTTCCGGAGAAACGAGTGACTGATTTTCTCGTCGGTTGTTCCGTCTTGTCCGTTCATCTACCCGCCGGCATTGCCGGATTCGGCCGCGGCCCTGAATCGTTGCCCTCGCAAATGAGACTGAAACGATTCTCCTATTGCCTGGTTTCTCGCCGGTTCGACGACTCGCCGGTGAGTAGCCCTCTCGTACTGGACTCCGGTTCGGAATCCGGCGAATCGAAGACGAACCGTCTCATTTACGCACCGTTCCGAGAGAATCCGTCGGGATCCAACGCCGCATTTCGAGAATTCTATTACCTTTCTCTTCGGAGAATCCTCATCGGTGGAAAGCCAGTGAAGTTTCCGTACAAGTATCTCGTGCCGGACTCCGCCGGAAACGGCGGTGCGATCATCGATTCCGGTTCGACGTTTACATTTCTCGATAAGCCGATTTTCGAAGCCGTAGCGGAGGAGTTGGAGAAGCAACTGGTGAAATATCCCCGAGCGACCGGCGTTGAAGCCCAGTCCGGTTTGAGGCCGTGCTTCGATATATCCAAGGAGGAGAAGGTGGAGTTTCCGGAACTGGTTTTGAAGTTTAAAGGCGGAGCGAAGCTGGCTTTGCCGCCGGCGAATTACTTGGCGTTGGTGACGGATTCCGGCGTGGTTTGCTTGACGATGATGACGGATGAGGCCGGAGGCGGCGGTGGGCCGGCGATTATAATGGGGGCGTTTCAGCAGCAGAATATTTTGGTGGAGTACGATTTGGCGAAGGAGAGAATCGGATTTCGAAAGCAGAGATGCACGTGA

Protein sequence

MEFLPIPFLFSIFLLLSTSSSSSTSPITLPLTTFPSAPVTDPWQNINFLVSASLTRAHHLKRPRTSPNSSIEKVSLFSRSYGAYSISLSFGTPPQNLSFVFDTGSSLVWFPCTAGYLCSNCSFPNSDTKTIAKFIPKLSSSAKIVGCRNRKCAWIFGPNLKSRCRNCNPKSRRCFDSCPGYGIQYGSGATAGFLLSETLDFPEKRVTDFLVGCSVLSVHLPAGIAGFGRGPESLPSQMRLKRFSYCLVSRRFDDSPVSSPLVLDSGSESGESKTNRLIYAPFRENPSGSNAAFREFYYLSLRRILIGGKPVKFPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPRATGVEAQSGLRPCFDISKEEKVEFPELVLKFKGGAKLALPPANYLALVTDSGVVCLTMMTDEAGGGGGPAIIMGAFQQQNILVEYDLAKERIGFRKQRCT

Homology

[Close]

BLAST of Spg013028 vs. NCBI nr
Match: XP_023543736.1 (probable aspartyl protease At4g16563 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 796.2 bits (2055), Expect = 1.5e-226
Identity = 391/462 (84.63%), Postives = 420/462 (90.91%), Query Frame = 0


Query: 1   MEFLPIPFLFSIFLLLSTSSSSSTSPITLPLTTFPSAPVTDPWQNINFLVSASLTRAHHL 60
           MEF PIPFL SI LLLS SSSSS++ +TLPLT FPS P T PW+NI  LVSASLTRA HL
Sbjct: 1   MEFFPIPFLLSIVLLLSASSSSSSTTVTLPLTVFPSLPFTHPWKNIKHLVSASLTRAQHL 60

Query: 61  KRPRTSPNSSIEKVSLFSRSYGAYSISLSFGTPPQNLSFVFDTGSSLVWFPCTAGYLCSN 120
           K PR   N+SI+ V+LF RSYGAYSISL+FGTPPQ+LS VFDTGSSLVWFPCTAGY CSN
Sbjct: 61  KTPRIKSNTSIQNVALFPRSYGAYSISLAFGTPPQSLSLVFDTGSSLVWFPCTAGYRCSN 120

Query: 121 CSFPNSDTKTIAKFIPKLSSSAKIVGCRNRKCAWIFGPNLKSRCRNCNPKSRRCFDSCPG 180
           CSFPN D  TI KFIPKLSSSAKI+GCRNRKC+WIFGPNLKS CR+C+P+SR+C D+CPG
Sbjct: 121 CSFPNVDAATIPKFIPKLSSSAKIIGCRNRKCSWIFGPNLKSLCRSCSPRSRKCSDTCPG 180

Query: 181 YGIQYGSGATAGFLLSETLDFPEKRVTDFLVGCSVLSVHLPAGIAGFGRGPESLPSQMRL 240
           YGIQYGSGATAGFLLSETLDFPEKRV DFLVGCSV+SVH PAGIAGFGRGPESLPSQM L
Sbjct: 181 YGIQYGSGATAGFLLSETLDFPEKRVPDFLVGCSVVSVHQPAGIAGFGRGPESLPSQMGL 240

Query: 241 KRFSYCLVSRRFDDSPVSSPLVLDSGSESGESKTNRLIYAPFRENPSGSNAAFREFYYLS 300
           KRFS+CLV R+FDDSPVSSPLVLDS SESGESK N LIYAPFRENPSGSNAAFRE+YYL+
Sbjct: 241 KRFSHCLVPRQFDDSPVSSPLVLDSSSESGESKNNSLIYAPFRENPSGSNAAFREYYYLT 300

Query: 301 LRRILIGGKPVKFPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPR 360
           LRRILIG KPVKFPYKYLVP+SAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPR
Sbjct: 301 LRRILIGRKPVKFPYKYLVPNSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPR 360

Query: 361 ATGVEAQSGLRPCFDISKEEKVEFPELVLKFKGGAKLALPPANYLALVTDSGVVCLTMMT 420
           A GVEA+SGLRPCFDISKEE VEFPEL+LKFKGGA LALPPANYLALVTD+GVVCLTM+T
Sbjct: 361 AKGVEAESGLRPCFDISKEESVEFPELILKFKGGATLALPPANYLALVTDTGVVCLTMIT 420

Query: 421 DEA--GGGGGPAIIMGAFQQQNILVEYDLAKERIGFRKQRCT 461
           D    GGGGGPAII GAFQQQN+LV+YDLAK+RIGFRKQRCT
Sbjct: 421 DVTFLGGGGGPAIIFGAFQQQNVLVQYDLAKDRIGFRKQRCT 462

[Close]

BLAST of Spg013028 vs. NCBI nr
Match: XP_022979057.1 (probable aspartyl protease At4g16563 [Cucurbita maxima])

HSP 1 Score: 792.0 bits (2044), Expect = 2.8e-225
Identity = 389/462 (84.20%), Postives = 419/462 (90.69%), Query Frame = 0


Query: 1   MEFLPIPFLFSIFLLLSTSSSSSTSPITLPLTTFPSAPVTDPWQNINFLVSASLTRAHHL 60
           MEF PI FL SI LLLS SSSSS+  +TLPLT FPS P+T PW+NI  LVSASL RA HL
Sbjct: 1   MEFFPIQFLLSIVLLLSASSSSSSITVTLPLTAFPSLPLTHPWKNIKHLVSASLARAQHL 60

Query: 61  KRPRTSPNSSIEKVSLFSRSYGAYSISLSFGTPPQNLSFVFDTGSSLVWFPCTAGYLCSN 120
           K P+T  N+SI+ V+LF RSYGAYSISL+FGTPPQ+LS VFDTGSSLVWFPCTAGY CSN
Sbjct: 61  KTPKTKSNTSIQNVALFPRSYGAYSISLAFGTPPQSLSLVFDTGSSLVWFPCTAGYRCSN 120

Query: 121 CSFPNSDTKTIAKFIPKLSSSAKIVGCRNRKCAWIFGPNLKSRCRNCNPKSRRCFDSCPG 180
           CSFPN D  TI KFIPKLSSSA+I+GCRNRKC+WIFGPNLKS CR+C+P+SR+C D+CPG
Sbjct: 121 CSFPNVDAATIPKFIPKLSSSARIIGCRNRKCSWIFGPNLKSSCRSCSPRSRKCSDTCPG 180

Query: 181 YGIQYGSGATAGFLLSETLDFPEKRVTDFLVGCSVLSVHLPAGIAGFGRGPESLPSQMRL 240
           YGIQYGSGATAGFLLSETLDFPEKRV DFLVGCSVLSVH PAGIAGFGRGPESLPSQM L
Sbjct: 181 YGIQYGSGATAGFLLSETLDFPEKRVPDFLVGCSVLSVHQPAGIAGFGRGPESLPSQMGL 240

Query: 241 KRFSYCLVSRRFDDSPVSSPLVLDSGSESGESKTNRLIYAPFRENPSGSNAAFREFYYLS 300
           KRFS+CLV R+FDDSPVSSPLVLDS  ESG+SKTN LIYAPFRENPSGSNAAFRE+YYL+
Sbjct: 241 KRFSHCLVPRQFDDSPVSSPLVLDSSPESGDSKTNSLIYAPFRENPSGSNAAFREYYYLT 300

Query: 301 LRRILIGGKPVKFPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPR 360
           LRRILIG KPVKFPYKYLVP+SAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPR
Sbjct: 301 LRRILIGRKPVKFPYKYLVPNSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPR 360

Query: 361 ATGVEAQSGLRPCFDISKEEKVEFPELVLKFKGGAKLALPPANYLALVTDSGVVCLTMMT 420
           A GVEA+SGLRPCFDISKEE VEFPEL+LKFKGGA LALPPANYLALVTD+GVVCLTM+T
Sbjct: 361 AKGVEAESGLRPCFDISKEESVEFPELILKFKGGATLALPPANYLALVTDTGVVCLTMIT 420

Query: 421 DE--AGGGGGPAIIMGAFQQQNILVEYDLAKERIGFRKQRCT 461
           D    GGGGGPAII GAFQQQN+LV+YDLAKERIGFRKQRCT
Sbjct: 421 DVNFLGGGGGPAIIFGAFQQQNVLVQYDLAKERIGFRKQRCT 462

[Close]

BLAST of Spg013028 vs. NCBI nr
Match: XP_022925946.1 (probable aspartyl protease At4g16563 [Cucurbita moschata] >KAG6604319.1 Aspartic proteinase nepenthesin-1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 787.3 bits (2032), Expect = 7.0e-224
Identity = 387/462 (83.77%), Postives = 417/462 (90.26%), Query Frame = 0


Query: 1   MEFLPIPFLFSIFLLLSTSSSSSTSPITLPLTTFPSAPVTDPWQNINFLVSASLTRAHHL 60
           MEF  IPFL SI LLLS SSSSS++ +TLPLT FPS P   PW+NI  LVSASLTRA HL
Sbjct: 1   MEFFLIPFLLSIVLLLSASSSSSSTTVTLPLTVFPSLPFAHPWKNIKHLVSASLTRAQHL 60

Query: 61  KRPRTSPNSSIEKVSLFSRSYGAYSISLSFGTPPQNLSFVFDTGSSLVWFPCTAGYLCSN 120
           K PRT  N+SI+ V+LF RSYGAYSISL+FGTPPQ+LS VFDTGSSLVWFPCTAGY CSN
Sbjct: 61  KTPRTKSNTSIQNVALFPRSYGAYSISLAFGTPPQSLSLVFDTGSSLVWFPCTAGYRCSN 120

Query: 121 CSFPNSDTKTIAKFIPKLSSSAKIVGCRNRKCAWIFGPNLKSRCRNCNPKSRRCFDSCPG 180
           CSFPN D  TI KFIPKLSSSAKI+GCRNRKC+WIFGPNLK+ CR+C+P+SR+C D+CPG
Sbjct: 121 CSFPNVDAATIPKFIPKLSSSAKIIGCRNRKCSWIFGPNLKTLCRSCSPRSRKCSDTCPG 180

Query: 181 YGIQYGSGATAGFLLSETLDFPEKRVTDFLVGCSVLSVHLPAGIAGFGRGPESLPSQMRL 240
           YGIQYGSGATAGFLLSETLDFPEKRV DFLVGCSV+SVH PAGIAGFGRGPESLPSQM L
Sbjct: 181 YGIQYGSGATAGFLLSETLDFPEKRVPDFLVGCSVVSVHQPAGIAGFGRGPESLPSQMGL 240

Query: 241 KRFSYCLVSRRFDDSPVSSPLVLDSGSESGESKTNRLIYAPFRENPSGSNAAFREFYYLS 300
           KRFS+CLV R+FDDSPVSSPLVLDS SESGESK N LIYAPFRENPSGSNAAFRE+YYL+
Sbjct: 241 KRFSHCLVPRQFDDSPVSSPLVLDSSSESGESKNNSLIYAPFRENPSGSNAAFREYYYLT 300

Query: 301 LRRILIGGKPVKFPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPR 360
           LRRILIG KPVKFPYKYLVP+SAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPR
Sbjct: 301 LRRILIGRKPVKFPYKYLVPNSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPR 360

Query: 361 ATGVEAQSGLRPCFDISKEEKVEFPELVLKFKGGAKLALPPANYLALVTDSGVVCLTMMT 420
           A GVEA+SGLRPCFDISKEE VEFPEL+LKFKGGA LALPP+NYLALV D+ VVCLTM+T
Sbjct: 361 AKGVEAESGLRPCFDISKEESVEFPELILKFKGGATLALPPSNYLALVADTSVVCLTMIT 420

Query: 421 DEA--GGGGGPAIIMGAFQQQNILVEYDLAKERIGFRKQRCT 461
           D    GGGGGPAII GAFQQQN+LV+YDLAKERIGFRKQRCT
Sbjct: 421 DVTFLGGGGGPAIIFGAFQQQNVLVQYDLAKERIGFRKQRCT 462

[Close]

BLAST of Spg013028 vs. NCBI nr
Match: KAG7034471.1 (Aspartic proteinase nepenthesin-1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 786.6 bits (2030), Expect = 1.2e-223
Identity = 387/462 (83.77%), Postives = 417/462 (90.26%), Query Frame = 0


Query: 1   MEFLPIPFLFSIFLLLSTSSSSSTSPITLPLTTFPSAPVTDPWQNINFLVSASLTRAHHL 60
           MEF  IPFL SI LLLS SSSSS++ +TLPLT FPS P   PW+NI  LVSASLTRA HL
Sbjct: 1   MEFFLIPFLLSIVLLLSASSSSSSTTVTLPLTVFPSLPFAHPWKNIKHLVSASLTRAQHL 60

Query: 61  KRPRTSPNSSIEKVSLFSRSYGAYSISLSFGTPPQNLSFVFDTGSSLVWFPCTAGYLCSN 120
           K PRT  N+SI+ V+LF RSYGAYSISL+FGTPPQ+LS VFDTGSSLVWFPCTAGY CSN
Sbjct: 61  KIPRTKSNTSIQNVALFPRSYGAYSISLAFGTPPQSLSLVFDTGSSLVWFPCTAGYRCSN 120

Query: 121 CSFPNSDTKTIAKFIPKLSSSAKIVGCRNRKCAWIFGPNLKSRCRNCNPKSRRCFDSCPG 180
           CSFPN D  TI KFIPKLSSSAKI+GCRNRKC+WIFGPNLK+ CR+C+P+SR+C D+CPG
Sbjct: 121 CSFPNVDAATIPKFIPKLSSSAKIIGCRNRKCSWIFGPNLKTLCRSCSPRSRKCSDTCPG 180

Query: 181 YGIQYGSGATAGFLLSETLDFPEKRVTDFLVGCSVLSVHLPAGIAGFGRGPESLPSQMRL 240
           YGIQYGSGATAGFLLSETLDFPEKRV DFLVGCSV+SVH PAGIAGFGRGPESLPSQM L
Sbjct: 181 YGIQYGSGATAGFLLSETLDFPEKRVPDFLVGCSVVSVHQPAGIAGFGRGPESLPSQMGL 240

Query: 241 KRFSYCLVSRRFDDSPVSSPLVLDSGSESGESKTNRLIYAPFRENPSGSNAAFREFYYLS 300
           KRFS+CLV R+FDDSPVSSPLVLDS SESGESK N LIYAPFRENPSGSNAAFRE+YYL+
Sbjct: 241 KRFSHCLVPRQFDDSPVSSPLVLDSSSESGESKNNSLIYAPFRENPSGSNAAFREYYYLT 300

Query: 301 LRRILIGGKPVKFPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPR 360
           LRRILIG KPVKFPYKYLVP+SAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPR
Sbjct: 301 LRRILIGRKPVKFPYKYLVPNSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPR 360

Query: 361 ATGVEAQSGLRPCFDISKEEKVEFPELVLKFKGGAKLALPPANYLALVTDSGVVCLTMMT 420
           A GVEA+SGLRPCFDISKEE VEFPEL+LKFKGGA LALPP+NYLALV D+ VVCLTM+T
Sbjct: 361 AKGVEAESGLRPCFDISKEESVEFPELILKFKGGATLALPPSNYLALVADTSVVCLTMIT 420

Query: 421 DEA--GGGGGPAIIMGAFQQQNILVEYDLAKERIGFRKQRCT 461
           D    GGGGGPAII GAFQQQN+LV+YDLAKERIGFRKQRCT
Sbjct: 421 DVTFLGGGGGPAIIFGAFQQQNVLVQYDLAKERIGFRKQRCT 462

[Close]

BLAST of Spg013028 vs. NCBI nr
Match: XP_038881211.1 (probable aspartyl protease At4g16563 [Benincasa hispida])

HSP 1 Score: 775.0 bits (2000), Expect = 3.6e-220
Identity = 390/463 (84.23%), Postives = 408/463 (88.12%), Query Frame = 0


Query: 1   MEFLPIPFLFSIFLLLSTSSSSSTSPITLPLTTFPSAPVT-DPWQNINFLVSASLTRAHH 60
           MEF PIPFLFSIFLLL TSSSSS S ITLPLT FPS P+T DP + IN+L+SASL RA H
Sbjct: 1   MEFFPIPFLFSIFLLLPTSSSSSISTITLPLTAFPSIPLTDDPLKIINYLLSASLNRAQH 60

Query: 61  LKRPRTSPNSSIEKVSLFSRSYGAYSISLSFGTPPQNLSFVFDTGSSLVWFPCTAGYLCS 120
           LK P+T    SI+ VSLFSRSYGAYSI+L+FGTPPQNLSFVFDTGSSLVWFPCTAGY CS
Sbjct: 61  LKNPQT---KSIQNVSLFSRSYGAYSITLAFGTPPQNLSFVFDTGSSLVWFPCTAGYRCS 120

Query: 121 NCSFPNSDTKTIAKFIPKLSSSAKIVGCRNRKCAWIFGPNLKSRCRNCNPKSRRCFDSCP 180
           NCSFPN D  TI KF+PKLSSSAKI+GCRN KCAWIFGPNL SRCRNCNPKSR C  SCP
Sbjct: 121 NCSFPNVDAATIPKFVPKLSSSAKIIGCRNPKCAWIFGPNLNSRCRNCNPKSRNCSGSCP 180

Query: 181 GYGIQYGSGATAGFLLSETLDFPEKRVTDFLVGCSVLSVHLPAGIAGFGRGPESLPSQMR 240
           GYGI YGSGATAGFLLSETLDFP+K V DFLVGCSV SVH PAGIAGFGR PESLPSQMR
Sbjct: 181 GYGILYGSGATAGFLLSETLDFPKKGVPDFLVGCSVSSVHQPAGIAGFGRAPESLPSQMR 240

Query: 241 LKRFSYCLVSRRFDDSPVSSPLVLDSGSESGESKTNRLIYAPFRENPSGSNAAFREFYYL 300
           LKRFSYCLVSR FDDSPVSSPLVLDSGSES +SKT   IYAPFRENPSGSNAAFRE+YYL
Sbjct: 241 LKRFSYCLVSRGFDDSPVSSPLVLDSGSESDDSKTESFIYAPFRENPSGSNAAFREYYYL 300

Query: 301 SLRRILIGGKPVKFPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYP 360
           SLRRILIGGKPVK PYKYL+PDSAG GGAIIDSGSTFTFLDKPIFEAVA ELEKQLVKYP
Sbjct: 301 SLRRILIGGKPVKIPYKYLMPDSAGKGGAIIDSGSTFTFLDKPIFEAVAGELEKQLVKYP 360

Query: 361 RATGVEAQSGLRPCFDISKEEKVEFPELVLKFKGGAKLALPPANYLALVTDSGVVCLTMM 420
           R   VE QSGLRPCFDISKE  VEFPELVLKFKGGAKL+LPP NYLALVTD+GVVCLTMM
Sbjct: 361 RTKSVEVQSGLRPCFDISKEVSVEFPELVLKFKGGAKLSLPPVNYLALVTDAGVVCLTMM 420

Query: 421 TD--EAGGGGGPAIIMGAFQQQNILVEYDLAKERIGFRKQRCT 461
           TD    GGG GPAII GAFQQQN+LVEYDLA+ RIGFRKQRCT
Sbjct: 421 TDVGVVGGGAGPAIIFGAFQQQNVLVEYDLARNRIGFRKQRCT 460

[Close]

BLAST of Spg013028 vs. ExPASy Swiss-Prot
Match: Q940R4 (Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g16563 PE=2 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 3.1e-49
Identity = 164/507 (32.35%), Postives = 230/507 (45.36%), Query Frame = 0


Query: 4   LPIPFLFSIFLLLSTSSSSSTSPITLPLTTFPSAPVTDPWQNINFLVSASLTRAHHLKRP 63
           L  P L  +   LSTS  SS+     PL    S+             S++  R HH K+ 
Sbjct: 25  LSTPLLLHLSHSLSTSKHSSS-----PLHLLKSSSSR----------SSARFRRHHHKQQ 84

Query: 64  RTSPNSSIEKVSLFSRSYGAYSISLSFGTPPQNLSFVFDTGSSLVWFPCTAGYLCSNCSF 123
           +       +++SL   S   Y ISLS G+    +S   DTGS LVWFPC   + C  C  
Sbjct: 85  Q-------QQLSLPISSGSDYLISLSVGSSSSAVSLYLDTGSDLVWFPCRP-FTCILC-- 144

Query: 124 PNSDTKTIAKFIP-KLSSSAKIVGCRNRKCAWIFGPNLKS-------------RCRNCNP 183
              ++K +    P  LSSSA  V C +  C+        S                +CN 
Sbjct: 145 ---ESKPLPPSPPSSLSSSATTVSCSSPSCSAAHSSLPSSDLCAISNCPLDFIETGDCNT 204

Query: 184 KSRRCFDSCPGYGIQYGSGATAGFLLSETLDFPEKRVTDFLVGCSVLSVHLPAGIAGFGR 243
            S      CP +   YG G+    L S++L  P   V++F  GC+  ++  P G+AGFGR
Sbjct: 205 SSY----PCPPFYYAYGDGSLVAKLYSDSLSLPSVSVSNFTFGCAHTTLAEPIGVAGFGR 264

Query: 244 GPESLPSQMRL------KRFSYCLVSRRFDDSPV--SSPLVL-----------------D 303
           G  SLP+Q+ +        FSYCLVS  FD   V   SPL+L                 D
Sbjct: 265 GRLSLPAQLAVHSPHLGNSFSYCLVSHSFDSDRVRRPSPLILGRFVDKKEKRVGTTDDHD 324

Query: 304 SGSESGESKTNRLIYAPFRENPSGSNAAFREFYYLSLRRILIGGKPVKFPYKYLVPDSAG 363
            G +  + K N  ++    ENP         FY +SL+ I IG + +  P      D  G
Sbjct: 325 DGDDE-KKKKNEFVFTEMLENPK-----HPYFYSVSLQGISIGKRNIPAPAMLRRIDKNG 384

Query: 364 NGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVK-YPRATGVEAQSGLRPCFDISKEEKVE 423
            GG ++DSG+TFT L    + +V EE + ++ + + RA  VE  SG+ PC+ +++  KV 
Sbjct: 385 GGGVVVDSGTTFTMLPAKFYNSVVEEFDSRVGRVHERADRVEPSSGMSPCYYLNQTVKV- 444

Query: 424 FPELVLKFKGG-AKLALPPANYLALVTDSG--------VVCLTMMT--DEAGGGGGPAII 460
            P LVL F G  + + LP  NY     D G        + CL +M   DE+   GG   I
Sbjct: 445 -PALVLHFAGNRSSVTLPRRNYFYEFMDGGDGKEEKRKIGCLMLMNGGDESELRGGTGAI 491

[Close]

BLAST of Spg013028 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 4.6e-37
Identity = 130/394 (32.99%), Postives = 172/394 (43.65%), Query Frame = 0


Query: 74  VSLFSRSYGAYSISLSFGTPPQNLSFVFDTGSSLVWFPCTAGYLCSNCSFPNSDTKTIAK 133
           VS  S+  G Y   L  GTP + +  V DTGS +VW  C     C + S P  D      
Sbjct: 132 VSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIFD------ 191

Query: 134 FIPKLSSSAKIVGCRNRKCAWIFGPNLKSRCRNCNPKSRRCFDSCPGYGIQYGSGA-TAG 193
             P+ S +   + C +  C        +     CN + + C      Y + YG G+ T G
Sbjct: 192 --PRKSKTYATIPCSSPHCR-------RLDSAGCNTRRKTCL-----YQVSYGDGSFTVG 251

Query: 194 FLLSETLDFPEKRVTDFLVGCSVLSVHL---PAGIAGFGRGPESLPSQMRLK---RFSYC 253
              +ETL F   RV    +GC   +  L    AG+ G G+G  S P Q   +   +FSYC
Sbjct: 252 DFSTETLTFRRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYC 311

Query: 254 LVSRRFDDSPVSSPLVLDSGSESGESKTNRLIYAPFRENPSGSNAAFREFYYLSLRRILI 313
           LV R     P        S    G +  +R+     R  P  SN     FYY+ L  I +
Sbjct: 312 LVDRSASSKP--------SSVVFGNAAVSRIA----RFTPLLSNPKLDTFYYVGLLGISV 371

Query: 314 GGKPVKFPYKYLVP-DSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPRATGVE 373
           GG  V      L   D  GNGG IIDSG++ T L +P + A+ +          RA    
Sbjct: 372 GGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDF- 431

Query: 374 AQSGLRPCFDISKEEKVEFPELVLKFKGGAKLALPPANYLALVTDSGVVCLTMMTDEAGG 433
             S    CFD+S   +V+ P +VL F+ GA ++LP  NYL  V  +G  C       AG 
Sbjct: 432 --SLFDTCFDLSNMNEVKVPTVVLHFR-GADVSLPATNYLIPVDTNGKFCFAF----AGT 484

Query: 434 GGGPAIIMGAFQQQNILVEYDLAKERIGFRKQRC 460
            GG +II G  QQQ   V YDLA  R+GF    C
Sbjct: 492 MGGLSII-GNIQQQGFRVVYDLASSRVGFAPGGC 484

[Close]

BLAST of Spg013028 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 1.9e-35
Identity = 119/387 (30.75%), Postives = 172/387 (44.44%), Query Frame = 0


Query: 82  GAYSISLSFGTPPQNLSFVFDTGSSLVWFPCTAGYLCSNCSFPNSDTKTIAKFIPKLSSS 141
           G Y ++LS GTP Q  S + DTGS L+W  C     C N S P         F P+ SSS
Sbjct: 93  GEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTP--------IFNPQGSSS 152

Query: 142 AKIVGCRNRKCAWIFGPNLKSRCRNCNPKSRRCFDSCPGYGIQYGSGA-TAGFLLSETLD 201
              + C ++ C  +  P               C ++   Y   YG G+ T G + +ETL 
Sbjct: 153 FSTLPCSSQLCQALSSPT--------------CSNNFCQYTYGYGDGSETQGSMGTETLT 212

Query: 202 FPEKRVTDFLVGCSVLSVHL----PAGIAGFGRGPESLPSQMRLKRFSYCLVSRRFDDSP 261
           F    + +   GC   +        AG+ G GRGP SLPSQ+ + +FSYC+       S 
Sbjct: 213 FGSVSIPNITFGCGENNQGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTP---IGSS 272

Query: 262 VSSPLVLDS--GSESGESKTNRLIYAPFRENPSGSNAAFREFYYLSLRRILIGGKPVKF- 321
             S L+L S   S +  S    LI           ++    FYY++L  + +G   +   
Sbjct: 273 TPSNLLLGSLANSVTAGSPNTTLI----------QSSQIPTFYYITLNGLSVGSTRLPID 332

Query: 322 PYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPRATGVEAQSGLRPC 381
           P  + +  + G GG IIDSG+T T+     +++V +E   Q +  P   G  + SG   C
Sbjct: 333 PSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQ-INLPVVNG--SSSGFDLC 392

Query: 382 FDI-SKEEKVEFPELVLKFKGGAKLALPPANYLALVTDSGVVCLTMMTDEAGGGGGPAII 441
           F   S    ++ P  V+ F GG  L LP  NY  +   +G++CL M +   G       I
Sbjct: 393 FQTPSDPSNLQIPTFVMHFDGG-DLELPSENYF-ISPSNGLICLAMGSSSQG-----MSI 434

Query: 442 MGAFQQQNILVEYDLAKERIGFRKQRC 460
            G  QQQN+LV YD     + F   +C
Sbjct: 453 FGNIQQQNMLVVYDTGNSVVSFASAQC 434

[Close]

BLAST of Spg013028 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 2.5e-35
Identity = 120/391 (30.69%), Postives = 172/391 (43.99%), Query Frame = 0


Query: 74  VSLFSRSYGAYSISLSFGTPPQNLSFVFDTGSSLVWFPCTAGYLCSNCSFPNSDTKTIAK 133
           VS  S+  G Y   +  GTP + +  V DTGS + W  C     C++C +  SD      
Sbjct: 152 VSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEP---CADC-YQQSD----PV 211

Query: 134 FIPKLSSSAKIVGCRNRKCAWIFGPNLKSRCRNCNPKSRRCFDSCPGYGIQYGSGA-TAG 193
           F P  SS+ K + C   +C+ +      S CR     S +C      Y + YG G+ T G
Sbjct: 212 FNPTSSSTYKSLTCSAPQCSLL----ETSACR-----SNKCL-----YQVSYGDGSFTVG 271

Query: 194 FLLSETLDFPEK-RVTDFLVGCSVLSVHL---PAGIAGFGRGPESLPSQMRLKRFSYCLV 253
            L ++T+ F    ++ +  +GC   +  L    AG+ G G G  S+ +QM+   FSYCLV
Sbjct: 272 ELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQMKATSFSYCLV 331

Query: 254 SRRFDDSPVSSPLVLDSGSESGESKTNRLIYAPFRENPSGSNAAFREFYYLSLRRILIGG 313
            R   DS  SS L  +S    G   T  L+           N     FYY+ L    +GG
Sbjct: 332 DR---DSGKSSSLDFNSVQLGGGDATAPLL----------RNKKIDTFYYVGLSGFSVGG 391

Query: 314 KPVKFPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPRATGVEAQS 373
           + V  P      D++G+GG I+D G+  T L    + ++ +   K  V   +  G  + S
Sbjct: 392 EKVVLPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKK--GSSSIS 451

Query: 374 GLRPCFDISKEEKVEFPELVLKFKGGAKLALPPANYLALVTDSGVVCLTMMTDEAGGGGG 433
               C+D S    V+ P +   F GG  L LP  NYL  V DSG  C       +     
Sbjct: 452 LFDTCYDFSSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSS---- 500

Query: 434 PAIIMGAFQQQNILVEYDLAKERIGFRKQRC 460
              I+G  QQQ   + YDL+K  IG    +C
Sbjct: 512 -LSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

[Close]

BLAST of Spg013028 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 3.1e-33
Identity = 121/388 (31.19%), Postives = 175/388 (45.10%), Query Frame = 0


Query: 82  GAYSISLSFGTPPQNLSFVFDTGSSLVWFPCTAGYLCSNCSFPNSDTKTIAKFIPKLSSS 141
           G Y ++++ GTP  + S + DTGS L+W  C     C+ C      ++    F P+ SSS
Sbjct: 94  GEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEP---CTQCF-----SQPTPIFNPQDSSS 153

Query: 142 AKIVGCRNRKCAWIFGPNLKSRCRNCNPKSRRCFDSCPGYGIQYGSGATA-GFLLSETLD 201
              + C ++ C               +  S  C ++   Y   YG G+T  G++ +ET  
Sbjct: 154 FSTLPCESQYCQ--------------DLPSETCNNNECQYTYGYGDGSTTQGYMATETFT 213

Query: 202 FPEKRVTDFLVGCSV----LSVHLPAGIAGFGRGPESLPSQMRLKRFSYCLVSRRFDDSP 261
           F    V +   GC            AG+ G G GP SLPSQ+ + +FSYC+ S       
Sbjct: 214 FETSSVPNIAFGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQLGVGQFSYCMTS-----YG 273

Query: 262 VSSPLVLDSGSESG----ESKTNRLIYAPFRENPSGSNAAFREFYYLSLRRILIGGKPVK 321
            SSP  L  GS +      S +  LI++    NP+        +YY++L+ I +GG  + 
Sbjct: 274 SSSPSTLALGSAASGVPEGSPSTTLIHSSL--NPT--------YYYITLQGITVGGDNLG 333

Query: 322 FPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPRATGVEAQSGLRP 381
            P         G GG IIDSG+T T+L +  + AVA+    Q +  P  T  E+ SGL  
Sbjct: 334 IPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQ-INLP--TVDESSSGLST 393

Query: 382 CF-DISKEEKVEFPELVLKFKGGAKLALPPANYLALVTDSGVVCLTMMTDEAGGGGGPAI 441
           CF   S    V+ PE+ ++F GG  L L   N L +    GV+CL M +    G      
Sbjct: 394 CFQQPSDGSTVQVPEISMQFDGGV-LNLGEQNIL-ISPAEGVICLAMGSSSQLG----IS 435

Query: 442 IMGAFQQQNILVEYDLAKERIGFRKQRC 460
           I G  QQQ   V YDL    + F   +C
Sbjct: 454 IFGNIQQQETQVLYDLQNLAVSFVPTQC 435

[Close]

BLAST of Spg013028 vs. ExPASy TrEMBL
Match: A0A6J1IMR7 (probable aspartyl protease At4g16563 OS=Cucurbita maxima OX=3661 GN=LOC111478813 PE=3 SV=1)

HSP 1 Score: 792.0 bits (2044), Expect = 1.4e-225
Identity = 389/462 (84.20%), Postives = 419/462 (90.69%), Query Frame = 0


Query: 1   MEFLPIPFLFSIFLLLSTSSSSSTSPITLPLTTFPSAPVTDPWQNINFLVSASLTRAHHL 60
           MEF PI FL SI LLLS SSSSS+  +TLPLT FPS P+T PW+NI  LVSASL RA HL
Sbjct: 1   MEFFPIQFLLSIVLLLSASSSSSSITVTLPLTAFPSLPLTHPWKNIKHLVSASLARAQHL 60

Query: 61  KRPRTSPNSSIEKVSLFSRSYGAYSISLSFGTPPQNLSFVFDTGSSLVWFPCTAGYLCSN 120
           K P+T  N+SI+ V+LF RSYGAYSISL+FGTPPQ+LS VFDTGSSLVWFPCTAGY CSN
Sbjct: 61  KTPKTKSNTSIQNVALFPRSYGAYSISLAFGTPPQSLSLVFDTGSSLVWFPCTAGYRCSN 120

Query: 121 CSFPNSDTKTIAKFIPKLSSSAKIVGCRNRKCAWIFGPNLKSRCRNCNPKSRRCFDSCPG 180
           CSFPN D  TI KFIPKLSSSA+I+GCRNRKC+WIFGPNLKS CR+C+P+SR+C D+CPG
Sbjct: 121 CSFPNVDAATIPKFIPKLSSSARIIGCRNRKCSWIFGPNLKSSCRSCSPRSRKCSDTCPG 180

Query: 181 YGIQYGSGATAGFLLSETLDFPEKRVTDFLVGCSVLSVHLPAGIAGFGRGPESLPSQMRL 240
           YGIQYGSGATAGFLLSETLDFPEKRV DFLVGCSVLSVH PAGIAGFGRGPESLPSQM L
Sbjct: 181 YGIQYGSGATAGFLLSETLDFPEKRVPDFLVGCSVLSVHQPAGIAGFGRGPESLPSQMGL 240

Query: 241 KRFSYCLVSRRFDDSPVSSPLVLDSGSESGESKTNRLIYAPFRENPSGSNAAFREFYYLS 300
           KRFS+CLV R+FDDSPVSSPLVLDS  ESG+SKTN LIYAPFRENPSGSNAAFRE+YYL+
Sbjct: 241 KRFSHCLVPRQFDDSPVSSPLVLDSSPESGDSKTNSLIYAPFRENPSGSNAAFREYYYLT 300

Query: 301 LRRILIGGKPVKFPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPR 360
           LRRILIG KPVKFPYKYLVP+SAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPR
Sbjct: 301 LRRILIGRKPVKFPYKYLVPNSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPR 360

Query: 361 ATGVEAQSGLRPCFDISKEEKVEFPELVLKFKGGAKLALPPANYLALVTDSGVVCLTMMT 420
           A GVEA+SGLRPCFDISKEE VEFPEL+LKFKGGA LALPPANYLALVTD+GVVCLTM+T
Sbjct: 361 AKGVEAESGLRPCFDISKEESVEFPELILKFKGGATLALPPANYLALVTDTGVVCLTMIT 420

Query: 421 DE--AGGGGGPAIIMGAFQQQNILVEYDLAKERIGFRKQRCT 461
           D    GGGGGPAII GAFQQQN+LV+YDLAKERIGFRKQRCT
Sbjct: 421 DVNFLGGGGGPAIIFGAFQQQNVLVQYDLAKERIGFRKQRCT 462

[Close]

BLAST of Spg013028 vs. ExPASy TrEMBL
Match: A0A6J1EDJ0 (probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC111433208 PE=3 SV=1)

HSP 1 Score: 787.3 bits (2032), Expect = 3.4e-224
Identity = 387/462 (83.77%), Postives = 417/462 (90.26%), Query Frame = 0


Query: 1   MEFLPIPFLFSIFLLLSTSSSSSTSPITLPLTTFPSAPVTDPWQNINFLVSASLTRAHHL 60
           MEF  IPFL SI LLLS SSSSS++ +TLPLT FPS P   PW+NI  LVSASLTRA HL
Sbjct: 1   MEFFLIPFLLSIVLLLSASSSSSSTTVTLPLTVFPSLPFAHPWKNIKHLVSASLTRAQHL 60

Query: 61  KRPRTSPNSSIEKVSLFSRSYGAYSISLSFGTPPQNLSFVFDTGSSLVWFPCTAGYLCSN 120
           K PRT  N+SI+ V+LF RSYGAYSISL+FGTPPQ+LS VFDTGSSLVWFPCTAGY CSN
Sbjct: 61  KTPRTKSNTSIQNVALFPRSYGAYSISLAFGTPPQSLSLVFDTGSSLVWFPCTAGYRCSN 120

Query: 121 CSFPNSDTKTIAKFIPKLSSSAKIVGCRNRKCAWIFGPNLKSRCRNCNPKSRRCFDSCPG 180
           CSFPN D  TI KFIPKLSSSAKI+GCRNRKC+WIFGPNLK+ CR+C+P+SR+C D+CPG
Sbjct: 121 CSFPNVDAATIPKFIPKLSSSAKIIGCRNRKCSWIFGPNLKTLCRSCSPRSRKCSDTCPG 180

Query: 181 YGIQYGSGATAGFLLSETLDFPEKRVTDFLVGCSVLSVHLPAGIAGFGRGPESLPSQMRL 240
           YGIQYGSGATAGFLLSETLDFPEKRV DFLVGCSV+SVH PAGIAGFGRGPESLPSQM L
Sbjct: 181 YGIQYGSGATAGFLLSETLDFPEKRVPDFLVGCSVVSVHQPAGIAGFGRGPESLPSQMGL 240

Query: 241 KRFSYCLVSRRFDDSPVSSPLVLDSGSESGESKTNRLIYAPFRENPSGSNAAFREFYYLS 300
           KRFS+CLV R+FDDSPVSSPLVLDS SESGESK N LIYAPFRENPSGSNAAFRE+YYL+
Sbjct: 241 KRFSHCLVPRQFDDSPVSSPLVLDSSSESGESKNNSLIYAPFRENPSGSNAAFREYYYLT 300

Query: 301 LRRILIGGKPVKFPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPR 360
           LRRILIG KPVKFPYKYLVP+SAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPR
Sbjct: 301 LRRILIGRKPVKFPYKYLVPNSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPR 360

Query: 361 ATGVEAQSGLRPCFDISKEEKVEFPELVLKFKGGAKLALPPANYLALVTDSGVVCLTMMT 420
           A GVEA+SGLRPCFDISKEE VEFPEL+LKFKGGA LALPP+NYLALV D+ VVCLTM+T
Sbjct: 361 AKGVEAESGLRPCFDISKEESVEFPELILKFKGGATLALPPSNYLALVADTSVVCLTMIT 420

Query: 421 DEA--GGGGGPAIIMGAFQQQNILVEYDLAKERIGFRKQRCT 461
           D    GGGGGPAII GAFQQQN+LV+YDLAKERIGFRKQRCT
Sbjct: 421 DVTFLGGGGGPAIIFGAFQQQNVLVQYDLAKERIGFRKQRCT 462

[Close]

BLAST of Spg013028 vs. ExPASy TrEMBL
Match: A0A0A0KHK2 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G454470 PE=3 SV=1)

HSP 1 Score: 768.1 bits (1982), Expect = 2.1e-218
Identity = 381/463 (82.29%), Postives = 412/463 (88.98%), Query Frame = 0


Query: 1   MEFLPIPFLFSIFLLLSTSSSSSTSPITLPLTTFPSAPVTDPWQNINFLVSASLTRAHHL 60
           MEFLPIPFLFSIFLLL TSSSSST+   LPLTTFPS   TDP++ IN L+SASL RA HL
Sbjct: 1   MEFLPIPFLFSIFLLLPTSSSSSTT--VLPLTTFPSVSFTDPFKTINLLLSASLNRAQHL 60

Query: 61  KRPRTSPNSSIEKVSLFSRSYGAYSISLSFGTPPQNLSFVFDTGSSLVWFPCTAGYLCSN 120
           K P++  N+SI+ VSLF RSYGAYS+SL+FGTPPQNLSF+FDTGSSLVWFPCTAGY CS 
Sbjct: 61  KTPQSKSNTSIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCSR 120

Query: 121 CSFPNSDTKTIAKFIPKLSSSAKIVGCRNRKCAWIFGPNLKSRCRNCNPKSRRCFDSCPG 180
           CSFP  D  TI+KF+PKLSSS K+VGCRN KCAWIFGPNLKSRCRNCN KSR+C DSCPG
Sbjct: 121 CSFPYVDPATISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCPG 180

Query: 181 YGIQYGSGATAGFLLSETLDFPEKRVTDFLVGCSVLSVHLPAGIAGFGRGPESLPSQMRL 240
           YG+QYGSGATAG LLSETLD   KRV DFLVGCSV+SVH PAGIAGFGRGPESLPSQMRL
Sbjct: 181 YGLQYGSGATAGILLSETLDLENKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRL 240

Query: 241 KRFSYCLVSRRFDDSPVSSPLVLDSGSESGESKTNRLIYAPFRENPSGSNAAFREFYYLS 300
           KRFS+CLVSR FDDSPVSSPLVLDSGSES ESKT   IYAPFRENPS SNAAFRE+YYLS
Sbjct: 241 KRFSHCLVSRGFDDSPVSSPLVLDSGSESDESKTKSFIYAPFRENPSVSNAAFREYYYLS 300

Query: 301 LRRILIGGKPVKFPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPR 360
           LRRILIGGKPVKFPYKYLVPDS GNGGAIIDSGSTFTFLDKPIFEA+A+ELEKQLVKYPR
Sbjct: 301 LRRILIGGKPVKFPYKYLVPDSTGNGGAIIDSGSTFTFLDKPIFEAIADELEKQLVKYPR 360

Query: 361 ATGVEAQSGLRPCFDISKEEK-VEFPELVLKFKGGAKLALPPANYLALVTDSGVVCLTMM 420
           A  VEAQSGLRPCF+I KEE+  EFP++VLKFKGG KL+L   NYLA+VTD GVVCLTMM
Sbjct: 361 AKDVEAQSGLRPCFNIPKEEESAEFPDVVLKFKGGGKLSLAAENYLAMVTDEGVVCLTMM 420

Query: 421 TDEA--GGGGGPAIIMGAFQQQNILVEYDLAKERIGFRKQRCT 461
           TDEA  GGGGGPAII+GAFQQQN+LVEYDLAK+RIGFRKQ+CT
Sbjct: 421 TDEAVVGGGGGPAIILGAFQQQNVLVEYDLAKQRIGFRKQKCT 461

[Close]

BLAST of Spg013028 vs. ExPASy TrEMBL
Match: A0A5A7SGF9 (Aspartic proteinase nepenthesin-2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold541G00670 PE=3 SV=1)

HSP 1 Score: 750.7 bits (1937), Expect = 3.5e-213
Identity = 371/463 (80.13%), Postives = 406/463 (87.69%), Query Frame = 0


Query: 1   MEFLPIPFLFSIFLLLSTSSSSSTSPITLPLTTFPSAPVTDPWQNINFLVSASLTRAHHL 60
           MEFLPIPFLFSIFLLL TSSSSS   ITLPL TFPS P TDP + IN L+SASL+RA HL
Sbjct: 1   MEFLPIPFLFSIFLLLPTSSSSS---ITLPLATFPSIPFTDPLKTINHLLSASLSRAQHL 60

Query: 61  KRPRTSPNSSIEKVSLFSRSYGAYSISLSFGTPPQNLSFVFDTGSSLVWFPCTAGYLCSN 120
           K P++  N+S E VSLF RSYGAY++SL+FGTPPQNLSF+FDTGSSLVWFPCTAGY C++
Sbjct: 61  KSPQSKSNTSTENVSLFPRSYGAYAVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCAH 120

Query: 121 CSFPNSDTKTIAKFIPKLSSSAKIVGCRNRKCAWIFGPNLKSRCRNCNPKSRRCFDSCPG 180
           CSFP+ D  TI+KF+PKLSSS KIVGCRN KCAWIFGPNLKSRCRNCNPKSR+C DSCPG
Sbjct: 121 CSFPHVDPATISKFVPKLSSSVKIVGCRNPKCAWIFGPNLKSRCRNCNPKSRKCSDSCPG 180

Query: 181 YGIQYGSGATAGFLLSETLDFPEKRVTDFLVGCSVLSVHLPAGIAGFGRGPESLPSQMRL 240
           YGIQYGSGATAG LLSETLD   KRV DFLVGCSV+SVH PAGIAGFGRGPESLPSQMRL
Sbjct: 181 YGIQYGSGATAGILLSETLDLQNKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRL 240

Query: 241 KRFSYCLVSRRFDDSPVSSPLVLDSGSESGESKTNRLIYAPFRENPSGSNAAFREFYYLS 300
           KRFS+CL+ R FDDSPVSSPLVLDSG ES ESKT   IYAPF+ENPS SN AFRE+YYLS
Sbjct: 241 KRFSHCLLPRGFDDSPVSSPLVLDSGPESDESKTKSFIYAPFQENPSRSNTAFREYYYLS 300

Query: 301 LRRILIGGKPVKFPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPR 360
           LRRILIGGKPVKFPYKYLVPDS G GGAIIDSGSTFTFLDKPIFEA+A ELEKQLVKYPR
Sbjct: 301 LRRILIGGKPVKFPYKYLVPDSTGKGGAIIDSGSTFTFLDKPIFEAIAGELEKQLVKYPR 360

Query: 361 ATGVEAQSGLRPCFDISKEEK-VEFPELVLKFKGGAKLALPPANYLALVTDSGVVCLTMM 420
           A  +EA++GLRPCF+ISKEE+  EFPE+ LKFKGG KL+LPP NYL +VTD+ VVCLTMM
Sbjct: 361 AKDIEAKTGLRPCFNISKEEESAEFPEVALKFKGGGKLSLPPENYLVMVTDANVVCLTMM 420

Query: 421 T--DEAGGGGGPAIIMGAFQQQNILVEYDLAKERIGFRKQRCT 461
           T  +  G GGGPAII GAFQQQN+LVEYDLAK+RIGFRKQ+CT
Sbjct: 421 TNAEVVGVGGGPAIIFGAFQQQNVLVEYDLAKQRIGFRKQKCT 460

[Close]

BLAST of Spg013028 vs. ExPASy TrEMBL
Match: A0A1S3CHV2 (aspartic proteinase nepenthesin-2-like OS=Cucumis melo OX=3656 GN=LOC103500932 PE=3 SV=1)

HSP 1 Score: 750.7 bits (1937), Expect = 3.5e-213
Identity = 371/463 (80.13%), Postives = 406/463 (87.69%), Query Frame = 0


Query: 1   MEFLPIPFLFSIFLLLSTSSSSSTSPITLPLTTFPSAPVTDPWQNINFLVSASLTRAHHL 60
           MEFLPIPFLFSIFLLL TSSSSS   ITLPL TFPS P TDP + IN L+SASL+RA HL
Sbjct: 1   MEFLPIPFLFSIFLLLPTSSSSS---ITLPLATFPSIPFTDPLKTINHLLSASLSRAQHL 60

Query: 61  KRPRTSPNSSIEKVSLFSRSYGAYSISLSFGTPPQNLSFVFDTGSSLVWFPCTAGYLCSN 120
           K P++  N+S E VSLF RSYGAY++SL+FGTPPQNLSF+FDTGSSLVWFPCTAGY C++
Sbjct: 61  KSPQSKSNTSTENVSLFPRSYGAYAVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCAH 120

Query: 121 CSFPNSDTKTIAKFIPKLSSSAKIVGCRNRKCAWIFGPNLKSRCRNCNPKSRRCFDSCPG 180
           CSFP+ D  TI+KF+PKLSSS KIVGCRN KCAWIFGPNLKSRCRNCNPKSR+C DSCPG
Sbjct: 121 CSFPHVDPATISKFVPKLSSSVKIVGCRNPKCAWIFGPNLKSRCRNCNPKSRKCSDSCPG 180

Query: 181 YGIQYGSGATAGFLLSETLDFPEKRVTDFLVGCSVLSVHLPAGIAGFGRGPESLPSQMRL 240
           YGIQYGSGATAG LLSETLD   KRV DFLVGCSV+SVH PAGIAGFGRGPESLPSQMRL
Sbjct: 181 YGIQYGSGATAGILLSETLDLQNKRVPDFLVGCSVMSVHQPAGIAGFGRGPESLPSQMRL 240

Query: 241 KRFSYCLVSRRFDDSPVSSPLVLDSGSESGESKTNRLIYAPFRENPSGSNAAFREFYYLS 300
           KRFS+CL+ R FDDSPVSSPLVLDSG ES ESKT   IYAPF+ENPS SN AFRE+YYLS
Sbjct: 241 KRFSHCLLPRGFDDSPVSSPLVLDSGPESDESKTKSFIYAPFQENPSRSNTAFREYYYLS 300

Query: 301 LRRILIGGKPVKFPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPR 360
           LRRILIGGKPVKFPYKYLVPDS G GGAIIDSGSTFTFLDKPIFEA+A ELEKQLVKYPR
Sbjct: 301 LRRILIGGKPVKFPYKYLVPDSTGKGGAIIDSGSTFTFLDKPIFEAIAGELEKQLVKYPR 360

Query: 361 ATGVEAQSGLRPCFDISKEEK-VEFPELVLKFKGGAKLALPPANYLALVTDSGVVCLTMM 420
           A  +EA++GLRPCF+ISKEE+  EFPE+ LKFKGG KL+LPP NYL +VTD+ VVCLTMM
Sbjct: 361 AKDIEAKTGLRPCFNISKEEESAEFPEVALKFKGGGKLSLPPENYLVMVTDANVVCLTMM 420

Query: 421 T--DEAGGGGGPAIIMGAFQQQNILVEYDLAKERIGFRKQRCT 461
           T  +  G GGGPAII GAFQQQN+LVEYDLAK+RIGFRKQ+CT
Sbjct: 421 TNAEVVGVGGGPAIIFGAFQQQNVLVEYDLAKQRIGFRKQKCT 460

[Close]

BLAST of Spg013028 vs. TAIR 10
Match: AT3G52500.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 489.6 bits (1259), Expect = 2.8e-138
Identity = 248/471 (52.65%), Postives = 327/471 (69.43%), Query Frame = 0


Query: 8   FLFSIFLLLSTSSSSSTSPITLPLTTFPSAPVT--DPWQNINFLVSASLTRAHHLKR--- 67
           F F IFL       S  S + LPL+ F  +  +  DP+ ++  L  +S+ RAH LK    
Sbjct: 7   FFFLIFL-------SVVSAVKLPLSPFSHSDQSPKDPYLSLRRLAESSIARAHKLKHGTS 66

Query: 68  ---------PRTSPNSSIEKVSLFSRSYGAYSISLSFGTPPQNLSFVFDTGSSLVWFPCT 127
                      T+ ++++ K  L ++SYG YS+SLSFGTP Q + FVFDTGSSLVW PCT
Sbjct: 67  IKPDEDALSSTTTASATVVKSPLSAKSYGGYSVSLSFGTPSQTIPFVFDTGSSLVWLPCT 126

Query: 128 AGYLCSNCSFPNSDTKTIAKFIPKLSSSAKIVGCRNRKCAWIFGPNLKSRCRNCNPKSRR 187
           + YLCS C F   D   I +FIPK SSS+KI+GC++ KC +++GPN+  +CR C+P +R 
Sbjct: 127 SRYLCSGCDFSGLDPTLIPRFIPKNSSSSKIIGCQSPKCQFLYGPNV--QCRGCDPNTRN 186

Query: 188 CFDSCPGYGIQYGSGATAGFLLSETLDFPEKRVTDFLVGCSVLSVHLPAGIAGFGRGPES 247
           C   CP Y +QYG G+TAG L++E LDFP+  V DF+VGCS++S   PAGIAGFGRGP S
Sbjct: 187 CTVGCPPYILQYGLGSTAGVLITEKLDFPDLTVPDFVVGCSIISTRQPAGIAGFGRGPVS 246

Query: 248 LPSQMRLKRFSYCLVSRRFDDSPVSSPLVLDSGS-ESGESKTNRLIYAPFRENPSGSNAA 307
           LPSQM LKRFS+CLVSRRFDD+ V++ L LD+GS  +  SKT  L Y PFR+NP+ SN A
Sbjct: 247 LPSQMNLKRFSHCLVSRRFDDTNVTTDLDLDTGSGHNSGSKTPGLTYTPFRKNPNVSNKA 306

Query: 308 FREFYYLSLRRILIGGKPVKFPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAVAEELE 367
           F E+YYL+LRRI +G K VK PYKYL P + G+GG+I+DSGSTFTF+++P+FE VAEE  
Sbjct: 307 FLEYYYLNLRRIYVGRKHVKIPYKYLAPGTNGDGGSIVDSGSTFTFMERPVFELVAEEFA 366

Query: 368 KQLVKYPRATGVEAQSGLRPCFDISKEEKVEFPELVLKFKGGAKLALPPANYLALVTDSG 427
            Q+  Y R   +E ++GL PCF+IS +  V  PEL+ +FKGGAKL LP +NY   V ++ 
Sbjct: 367 SQMSNYTREKDLEKETGLGPCFNISGKGDVTVPELIFEFKGGAKLELPLSNYFTFVGNTD 426

Query: 428 VVCLTMMTDEA---GGGGGPAIIMGAFQQQNILVEYDLAKERIGFRKQRCT 461
            VCLT+++D+     GG GPAII+G+FQQQN LVEYDL  +R GF K++C+
Sbjct: 427 TVCLTVVSDKTVNPSGGTGPAIILGSFQQQNYLVEYDLENDRFGFAKKKCS 468

[Close]

BLAST of Spg013028 vs. TAIR 10
Match: AT5G45120.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 201.1 bits (510), Expect = 2.0e-51
Identity = 153/480 (31.87%), Postives = 226/480 (47.08%), Query Frame = 0


Query: 8   FLF-SIFLLLSTSSSSSTSPITLPLTTFPSAPVTDPWQNINFLVSASLTRAHHLKRPRTS 67
           FLF  I LLL+T++ +       P ++  S  V    ++   L +        +K+P +S
Sbjct: 9   FLFLLITLLLNTTNKTQARQHKNPSSSSSSFLVLTLTKSSVSLPTPKSQTQERIKKPLSS 68

Query: 68  PNSSIEKVSLFSRSYGAYSISLSFGTPPQNLSFVFDTGSSLVWFPC-TAGYLCSNC-SFP 127
            +  +E +         Y I+L+ GTPPQ +    DTGS L W PC    + C  C    
Sbjct: 69  VDVVMEPL---REVRDGYLITLNIGTPPQAVQVYLDTGSDLTWVPCGNLSFDCIECYDLK 128

Query: 128 NSDTKTIAKFIPKLSSSAKIVGCRNRKCAWI------FGPNLKSRCRNCNPKSRRCFDSC 187
           N+D K+ + F P  SS++    C +  C  I      F P   + C         C   C
Sbjct: 129 NNDLKSPSVFSPLHSSTSFRDSCASSFCVEIHSSDNPFDPCAVAGCSVSMLLKSTCVRPC 188

Query: 188 PGYGIQYGSGA-TAGFLLSETLDFPEKRVTDFLVGCSVLSVHLPAGIAGFGRGPESLPSQ 247
           P +   YG G   +G L  + L    + V  F  GC   +   P GIAGFGRG  SLPSQ
Sbjct: 189 PSFAYTYGEGGLISGILTRDILKARTRDVPRFSFGCVTSTYREPIGIAGFGRGLLSLPSQ 248

Query: 248 MRL--KRFSYCLVSRRFDDSP-VSSPLVLDSGSESGESKTNRLIYAPFRENPSGSNAAFR 307
           +    K FS+C +  +F ++P +SSPL+L + + S  + T+ L + P    P   N+   
Sbjct: 249 LGFLEKGFSHCFLPFKFVNNPNISSPLILGASALS-INLTDSLQFTPMLNTPMYPNS--- 308

Query: 308 EFYYLSLRRILIGGK--PVKFPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAVAEELE 367
             YY+ L  I IG    P + P      DS GNGG ++DSG+T+T L +P +  +   L+
Sbjct: 309 --YYIGLESITIGTNITPTQVPLTLRQFDSQGNGGMLVDSGTTYTHLPEPFYSQLLTTLQ 368

Query: 368 KQLVKYPRATGVEAQSGLRPCF----------DISKEEKVEFPELVLKFKGGAKLALPPA 427
              + YPRAT  E+++G   C+           +  +  + FP +   F   A L LP  
Sbjct: 369 -STITYPRATETESRTGFDLCYKVPCPNNNLTSLENDVMMIFPSITFHFLNNATLLLPQG 428

Query: 428 N---YLALVTDSGVVCLTMMTDEAGGGGGPAIIMGAFQQQNILVEYDLAKERIGFRKQRC 460
           N    ++  +D  VV   +  +   G  GPA + G+FQQQN+ V YDL KERIGF+   C
Sbjct: 429 NSFYAMSAPSDGSVVQCLLFQNMEDGDYGPAGVFGSFQQQNVKVVYDLEKERIGFQAMDC 478

[Close]

BLAST of Spg013028 vs. TAIR 10
Match: AT4G16563.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 197.6 bits (501), Expect = 2.2e-50
Identity = 164/507 (32.35%), Postives = 230/507 (45.36%), Query Frame = 0


Query: 4   LPIPFLFSIFLLLSTSSSSSTSPITLPLTTFPSAPVTDPWQNINFLVSASLTRAHHLKRP 63
           L  P L  +   LSTS  SS+     PL    S+             S++  R HH K+ 
Sbjct: 25  LSTPLLLHLSHSLSTSKHSSS-----PLHLLKSSSSR----------SSARFRRHHHKQQ 84

Query: 64  RTSPNSSIEKVSLFSRSYGAYSISLSFGTPPQNLSFVFDTGSSLVWFPCTAGYLCSNCSF 123
           +       +++SL   S   Y ISLS G+    +S   DTGS LVWFPC   + C  C  
Sbjct: 85  Q-------QQLSLPISSGSDYLISLSVGSSSSAVSLYLDTGSDLVWFPCRP-FTCILC-- 144

Query: 124 PNSDTKTIAKFIP-KLSSSAKIVGCRNRKCAWIFGPNLKS-------------RCRNCNP 183
              ++K +    P  LSSSA  V C +  C+        S                +CN 
Sbjct: 145 ---ESKPLPPSPPSSLSSSATTVSCSSPSCSAAHSSLPSSDLCAISNCPLDFIETGDCNT 204

Query: 184 KSRRCFDSCPGYGIQYGSGATAGFLLSETLDFPEKRVTDFLVGCSVLSVHLPAGIAGFGR 243
            S      CP +   YG G+    L S++L  P   V++F  GC+  ++  P G+AGFGR
Sbjct: 205 SSY----PCPPFYYAYGDGSLVAKLYSDSLSLPSVSVSNFTFGCAHTTLAEPIGVAGFGR 264

Query: 244 GPESLPSQMRL------KRFSYCLVSRRFDDSPV--SSPLVL-----------------D 303
           G  SLP+Q+ +        FSYCLVS  FD   V   SPL+L                 D
Sbjct: 265 GRLSLPAQLAVHSPHLGNSFSYCLVSHSFDSDRVRRPSPLILGRFVDKKEKRVGTTDDHD 324

Query: 304 SGSESGESKTNRLIYAPFRENPSGSNAAFREFYYLSLRRILIGGKPVKFPYKYLVPDSAG 363
            G +  + K N  ++    ENP         FY +SL+ I IG + +  P      D  G
Sbjct: 325 DGDDE-KKKKNEFVFTEMLENPK-----HPYFYSVSLQGISIGKRNIPAPAMLRRIDKNG 384

Query: 364 NGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVK-YPRATGVEAQSGLRPCFDISKEEKVE 423
            GG ++DSG+TFT L    + +V EE + ++ + + RA  VE  SG+ PC+ +++  KV 
Sbjct: 385 GGGVVVDSGTTFTMLPAKFYNSVVEEFDSRVGRVHERADRVEPSSGMSPCYYLNQTVKV- 444

Query: 424 FPELVLKFKGG-AKLALPPANYLALVTDSG--------VVCLTMMT--DEAGGGGGPAII 460
            P LVL F G  + + LP  NY     D G        + CL +M   DE+   GG   I
Sbjct: 445 -PALVLHFAGNRSSVTLPRRNYFYEFMDGGDGKEEKRKIGCLMLMNGGDESELRGGTGAI 491

[Close]

BLAST of Spg013028 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 175.3 bits (443), Expect = 1.2e-43
Identity = 140/424 (33.02%), Postives = 190/424 (44.81%), Query Frame = 0


Query: 44  QNINFLVSASLTRAHHLKRPRTSPNSSIEKVSLFSRSYGAYSISLSFGTPPQNLSFVFDT 103
           ++I  L + S  R    + PRT+   S   +S  S+  G Y + L  GTP  N+  V DT
Sbjct: 95  KSITSLAAVSTGRNATKRTPRTAGGFSGAVISGLSQGSGEYFMRLGVGTPATNVYMVLDT 154

Query: 104 GSSLVWFPCTAGYLCSNCSFPNSDTKTIAKFIPKLSSSAKIVGCRNRKCAWIFGPNLKSR 163
           GS +VW  C+    C N        +T A F PK S +   V C +R C       L   
Sbjct: 155 GSDVVWLQCSPCKACYN--------QTDAIFDPKKSKTFATVPCGSRLCR-----RLDDS 214

Query: 164 CRNCNPKSRRCFDSCPGYGIQYGSGA-TAGFLLSETLDFPEKRVTDFLVGCSVLSVHL-- 223
                 +S+ C      Y + YG G+ T G   +ETL F   RV    +GC   +  L  
Sbjct: 215 SECVTRRSKTCL-----YQVSYGDGSFTEGDFSTETLTFHGARVDHVPLGCGHDNEGLFV 274

Query: 224 -PAGIAGFGRGPESLPSQMRLK---RFSYCLVSRRFDDSPVSSPLVLDSGSESGESKTNR 283
             AG+ G GRG  S PSQ + +   +FSYCLV R    S    P  +  G+ +   KT+ 
Sbjct: 275 GAAGLLGLGRGGLSFPSQTKNRYNGKFSYCLVDRTSSGSSSKPPSTIVFGN-AAVPKTS- 334

Query: 284 LIYAPFRENPSGSNAAFREFYYLSLRRILIGGKPVK-FPYKYLVPDSAGNGGAIIDSGST 343
            ++ P   NP         FYYL L  I +GG  V          D+ GNGG IIDSG++
Sbjct: 335 -VFTPLLTNPK-----LDTFYYLQLLGISVGGSRVPGVSESQFKLDATGNGGVIIDSGTS 394

Query: 344 FTFLDKPIFEAVAEELEKQLVKYPRATGVEAQSGLRPCFDISKEEKVEFPELVLKFKGGA 403
            T L +P + A+ +       K  RA    + S    CFD+S    V+ P +V  F GG 
Sbjct: 395 VTRLTQPAYVALRDAFRLGATKLKRA---PSYSLFDTCFDLSGMTTVKVPTVVFHF-GGG 454

Query: 404 KLALPPANYLALVTDSGVVCLTMMTDEAGGGGGPAIIMGAFQQQNILVEYDLAKERIGFR 460
           +++LP +NYL  V   G  C         G  G   I+G  QQQ   V YDL   R+GF 
Sbjct: 455 EVSLPASNYLIPVNTEGRFCFAF-----AGTMGSLSIIGNIQQQGFRVAYDLVGSRVGFL 483

[Close]

BLAST of Spg013028 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 160.2 bits (404), Expect = 3.9e-39
Identity = 121/390 (31.03%), Postives = 171/390 (43.85%), Query Frame = 0


Query: 74  VSLFSRSYGAYSISLSFGTPPQNLSFVFDTGSSLVWFPCTAGYLCSNCSFPNSDTKTIAK 133
           +S  ++  G Y   +  G P + +  V DTGS + W  CT    C++C       +T   
Sbjct: 138 ISGTTQGSGEYFTRVGIGKPAREVYMVLDTGSDVNWLQCTP---CADCYH-----QTEPI 197

Query: 134 FIPKLSSSAKIVGCRNRKCAWIFGPNLKSRCRNCNPKSRRCFDSCPGYGIQYGSGA-TAG 193
           F P  SSS + + C   +C  +      S CRN       C      Y + YG G+ T G
Sbjct: 198 FEPSSSSSYEPLSCDTPQCNAL----EVSECRNAT-----CL-----YEVSYGDGSYTVG 257

Query: 194 FLLSETLDFPEKRVTDFLVGCSVLSVHL---PAGIAGFGRGPESLPSQMRLKRFSYCLVS 253
              +ETL      V +  VGC   +  L    AG+ G G G  +LPSQ+    FSYCLV 
Sbjct: 258 DFATETLTIGSTLVQNVAVGCGHSNEGLFVGAAGLLGLGGGLLALPSQLNTTSFSYCLVD 317

Query: 254 RRFDDSPVSSPLVLDSGSESGESKTNRLIYAPFRENPSGSNAAFREFYYLSLRRILIGGK 313
           R  D +         S  + G S +   + AP   N          FYYL L  I +GG+
Sbjct: 318 RDSDSA---------STVDFGTSLSPDAVVAPLLRNHQ-----LDTFYYLGLTGISVGGE 377

Query: 314 PVKFPYKYLVPDSAGNGGAIIDSGSTFTFLDKPIFEAVAEELEKQLVKYPRATGVEAQSG 373
            ++ P      D +G+GG IIDSG+  T L   I+ ++ +   K  +   +A GV   + 
Sbjct: 378 LLQIPQSSFEMDESGSGGIIIDSGTAVTRLQTEIYNSLRDSFVKGTLDLEKAAGV---AM 437

Query: 374 LRPCFDISKEEKVEFPELVLKFKGGAKLALPPANYLALVTDSGVVCLTMMTDEAGGGGGP 433
              C+++S +  VE P +   F GG  LALP  NY+  V   G  CL      +      
Sbjct: 438 FDTCYNLSAKTTVEVPTVAFHFPGGKMLALPAKNYMIPVDSVGTFCLAFAPTASS----- 483

Query: 434 AIIMGAFQQQNILVEYDLAKERIGFRKQRC 460
             I+G  QQQ   V +DLA   IGF   +C
Sbjct: 498 LAIIGNVQQQGTRVTFDLANSLIGFSSNKC 483

The following BLAST results are available for this feature:

BLAST of Spg013028 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023543736.1	1.5e-226	84.63	probable aspartyl protease At4g16563 [Cucurbita pepo subsp. pepo]	[more]
XP_022979057.1	2.8e-225	84.20	probable aspartyl protease At4g16563 [Cucurbita maxima]	[more]
XP_022925946.1	7.0e-224	83.77	probable aspartyl protease At4g16563 [Cucurbita moschata] >KAG6604319.1 Aspartic...	[more]
KAG7034471.1	1.2e-223	83.77	Aspartic proteinase nepenthesin-1, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_038881211.1	3.6e-220	84.23	probable aspartyl protease At4g16563 [Benincasa hispida]	[more]

BLAST of Spg013028 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q940R4	3.1e-49	32.35	Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g1656...	[more]
Q9LNJ3	4.6e-37	32.99	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q766C3	1.9e-35	30.75	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q9LS40	2.5e-35	30.69	Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q766C2	3.1e-33	31.19	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]

BLAST of Spg013028 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IMR7	1.4e-225	84.20	probable aspartyl protease At4g16563 OS=Cucurbita maxima OX=3661 GN=LOC111478813...	[more]
A0A6J1EDJ0	3.4e-224	83.77	probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC1114332...	[more]
A0A0A0KHK2	2.1e-218	82.29	Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G45447...	[more]
A0A5A7SGF9	3.5e-213	80.13	Aspartic proteinase nepenthesin-2-like OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3CHV2	3.5e-213	80.13	aspartic proteinase nepenthesin-2-like OS=Cucumis melo OX=3656 GN=LOC103500932 P...	[more]

BLAST of Spg013028 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G52500.1	2.8e-138	52.65	Eukaryotic aspartyl protease family protein	[more]
AT5G45120.1	2.0e-51	31.88	Eukaryotic aspartyl protease family protein	[more]
AT4G16563.1	2.2e-50	32.35	Eukaryotic aspartyl protease family protein	[more]
AT3G61820.1	1.2e-43	33.02	Eukaryotic aspartyl protease family protein	[more]
AT1G25510.1	3.9e-39	31.03	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001461	Aspartic peptidase A1 family	PRINTS	PR00792	PEPSIN	coord: 328..339 score: 36.31 coord: 90..110 score: 51.74
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 59..267 e-value: 2.6E-31 score: 111.0
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 268..460 e-value: 1.5E-50 score: 173.5
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 79..459
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 84..254 e-value: 4.9E-28 score: 98.5
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 296..455 e-value: 2.1E-36 score: 125.2
None	No IPR available	PANTHER	PTHR47967:SF36	BNACNNG47670D PROTEIN	coord: 9..459
None	No IPR available	PANTHER	PTHR47967	OS07G0603500 PROTEIN-RELATED	coord: 9..459
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 99..110
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 84..455 score: 35.38715
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 83..459 e-value: 2.10637E-80 score: 248.332

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg013028.1	Spg013028.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004190	aspartic-type endopeptidase activity