Spg012974 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg012974 vs. NCBI nr
Match: XP_038881642.1 (uncharacterized protein LOC120073098 [Benincasa hispida])

HSP 1 Score: 989.6 bits (2557), Expect = 1.0e-284
Identity = 489/521 (93.86%), Postives = 500/521 (95.97%), Query Frame = 0

Query: 1   MGSMSGKFASLRNLLVRVLLFSILVILGRFAYIVTIAGESCSIGDFCFFSLPENFNFMIA 60
           MGSMSGKFASLRNLLVRVLLFSILVI+GRFAYIVTIAGESCSIGDFCFFSLPENFNFMIA
Sbjct: 1   MGSMSGKFASLRNLLVRVLLFSILVIVGRFAYIVTIAGESCSIGDFCFFSLPENFNFMIA 60

Query: 61  GGGGRAGSAALFTDNADPRVIGPPRPDLYSSEDWIKTVQFYSSVFQDLITEGYLSPHSKS 120
           GGGGRAGSAALFTDNADPRVIGPPRPDLYSSEDWIKTVQFYSSVFQDLITEGYLSP+SKS
Sbjct: 61  GGGGRAGSAALFTDNADPRVIGPPRPDLYSSEDWIKTVQFYSSVFQDLITEGYLSPNSKS 120

Query: 121 LCVETPAGADVSALKEIGVSDSVGIFKKALKPLVIKGEGHRIPFEDKTFDFVFSGGGRLD 180
           LC+ETP GADVSALKEIGVSDSVGIFKKA KPLVIKGE HRIPFE  TFDFVFSGGGRLD
Sbjct: 121 LCIETPGGADVSALKEIGVSDSVGIFKKASKPLVIKGEAHRIPFEGNTFDFVFSGGGRLD 180

Query: 181 ISRRASDFASEIGRILKPEGFAVVHVSAKDTYSFNSFVDLFNCCKIVKSKDIDVVLPSMP 240
           ISRR  DFASEI RILKPEGFAVVHVSAKDTYSFNSFVDLFNCCKI+KSKDID+VLPSMP
Sbjct: 181 ISRRPHDFASEIARILKPEGFAVVHVSAKDTYSFNSFVDLFNCCKILKSKDIDIVLPSMP 240

Query: 241 FIREYVLKKEYRILENGLQKLDGDSS---KKNCSVPGFKLSLVRNAEPLILEEPLKPWLT 300
           F REYVLKKEYRILENGLQKLDG+      KNCSVPG+KL+LVRNAEPLILEEPLKPWLT
Sbjct: 241 FTREYVLKKEYRILENGLQKLDGNGDGDFYKNCSVPGYKLALVRNAEPLILEEPLKPWLT 300

Query: 301 LKRNIQNIKYLPSMAEISFKNRYVYVDVGARSYGSSIGSWFKKQYPKQNKTFEVYAIEAD 360
           LKRNIQNIKYLPSMAEISFKNRYVYVDVGARSYGSSIGSWFKKQYPKQNKTFEVYAIEAD
Sbjct: 301 LKRNIQNIKYLPSMAEISFKNRYVYVDVGARSYGSSIGSWFKKQYPKQNKTFEVYAIEAD 360

Query: 361 QTFHEQYRSKKGVTLLPYAAWVRNETLAFEINKDPGQEMEEK-GSRGMGRIQPVQSSGHF 420
           QTFHEQY+SKKGVTLLPYAAWVRNETLAFEINKDP QE E+K GSRGMGRIQPVQSS  F
Sbjct: 361 QTFHEQYKSKKGVTLLPYAAWVRNETLAFEINKDPAQEKEDKGGSRGMGRIQPVQSSSKF 420

Query: 421 DGEVNQIQGFDFANWLKNTVSEKDFVVLKMDVEGTEFELIPRLFETGAICLIDEMFLECH 480
           DGEVNQIQGFDFANWLKNTVSEKDFVVLKMDVEGTEFELIPRLFETGAICLIDEMFLECH
Sbjct: 421 DGEVNQIQGFDFANWLKNTVSEKDFVVLKMDVEGTEFELIPRLFETGAICLIDEMFLECH 480

Query: 481 YNRWQRCCPGQRSTKYEKTYSQCIELFASLRQSGVLVHQWW 518
           YNRWQRCCPGQRSTKYEKTYSQCI+L ASLRQSGVLVHQWW
Sbjct: 481 YNRWQRCCPGQRSTKYEKTYSQCIDLLASLRQSGVLVHQWW 521

BLAST of Spg012974 vs. NCBI nr
Match: XP_022978145.1 (uncharacterized protein LOC111478211 [Cucurbita maxima])

HSP 1 Score: 987.6 bits (2552), Expect = 4.0e-284
Identity = 486/517 (94.00%), Postives = 500/517 (96.71%), Query Frame = 0

Query: 1   MGSMSGKFASLRNLLVRVLLFSILVILGRFAYIVTIAGESCSIGDFCFFSLPENFNFMIA 60
           MGS+SGKFASLRNLLVRVLLFSILVILGRFAYIVTIAGESC IGDFCFFSLPENFNFMIA
Sbjct: 1   MGSVSGKFASLRNLLVRVLLFSILVILGRFAYIVTIAGESCRIGDFCFFSLPENFNFMIA 60

Query: 61  GGGGRAGSAALFTDNADPRVIGPPRPDLYSSEDWIKTVQFYSSVFQDLITEGYLSPHSKS 120
            GGGRAGSAALFTDN D RVIGPPRPDLYSSEDWIKTVQFYSSVFQDL+TEGYLS +SK+
Sbjct: 61  -GGGRAGSAALFTDNTDSRVIGPPRPDLYSSEDWIKTVQFYSSVFQDLVTEGYLSSNSKA 120

Query: 121 LCVETPAGADVSALKEIGVSDSVGIFKKALKPLVIKGEGHRIPFEDKTFDFVFSGGGRLD 180
           LC+ETPAGADVSALKEIGVSDSVGIFKKALKP VIKGE HRIPFEDKTFDFVFSGGGRLD
Sbjct: 121 LCIETPAGADVSALKEIGVSDSVGIFKKALKPHVIKGEAHRIPFEDKTFDFVFSGGGRLD 180

Query: 181 ISRRASDFASEIGRILKPEGFAVVHVSAKDTYSFNSFVDLFNCCKIVKSKDIDVVLPSMP 240
           IS+R  DFASEI RILKPEGFAVVHVSAKDTYSFNSFVDLFNCCKI+KSKDID  LPSMP
Sbjct: 181 ISQRPYDFASEIARILKPEGFAVVHVSAKDTYSFNSFVDLFNCCKILKSKDIDNFLPSMP 240

Query: 241 FIREYVLKKEYRILENGLQKLDGDSSKKNCSVPGFKLSLVRNAEPLILEEPLKPWLTLKR 300
           F REYVLKKEYRILEN LQKLDGDS KKNCSVPG+KLSLVRNAEPLILEEPLKPWLTLKR
Sbjct: 241 FTREYVLKKEYRILENRLQKLDGDSYKKNCSVPGYKLSLVRNAEPLILEEPLKPWLTLKR 300

Query: 301 NIQNIKYLPSMAEISFKNRYVYVDVGARSYGSSIGSWFKKQYPKQNKTFEVYAIEADQTF 360
           NIQNIKYLPSMAEISFKN+YVYVDVGARSYGSSIGSWFKKQYPKQNKTFEVYAIEADQTF
Sbjct: 301 NIQNIKYLPSMAEISFKNKYVYVDVGARSYGSSIGSWFKKQYPKQNKTFEVYAIEADQTF 360

Query: 361 HEQYRSKKGVTLLPYAAWVRNETLAFEINKDPGQEMEEKGSRGMGRIQPVQSSGHFDGEV 420
           HEQYRSKK VTLLPYAAWVRNETLAFEINKDPGQEME+KGSRGMGRIQPVQSSGHF+GEV
Sbjct: 361 HEQYRSKKRVTLLPYAAWVRNETLAFEINKDPGQEMEDKGSRGMGRIQPVQSSGHFNGEV 420

Query: 421 NQIQGFDFANWLKNTVSEKDFVVLKMDVEGTEFELIPRLFETGAICLIDEMFLECHYNRW 480
           NQIQGFDFANWLKNTVSEKDFVVLKMDVEGTEFELIPRLFETGAICLIDEMFLECHYNRW
Sbjct: 421 NQIQGFDFANWLKNTVSEKDFVVLKMDVEGTEFELIPRLFETGAICLIDEMFLECHYNRW 480

Query: 481 QRCCPGQRSTKYEKTYSQCIELFASLRQSGVLVHQWW 518
           QRCCPG+RS KYEKTYS+CI+LFASLRQSGVLVHQWW
Sbjct: 481 QRCCPGERSRKYEKTYSECIDLFASLRQSGVLVHQWW 516

BLAST of Spg012974 vs. NCBI nr
Match: XP_022144100.1 (uncharacterized protein LOC111013875 [Momordica charantia])

HSP 1 Score: 980.3 bits (2533), Expect = 6.3e-282
Identity = 480/517 (92.84%), Postives = 496/517 (95.94%), Query Frame = 0

Query: 1   MGSMSGKFASLRNLLVRVLLFSILVILGRFAYIVTIAGESCSIGDFCFFSLPENFNFMIA 60
           MGSMSGKFASLRNLLVRVLLFSIL+I+GRFAYIVTI GESCSIGDFCFFSLPENFNFMIA
Sbjct: 1   MGSMSGKFASLRNLLVRVLLFSILIIIGRFAYIVTITGESCSIGDFCFFSLPENFNFMIA 60

Query: 61  GGGGRAGSAALFTDNADPRVIGPPRPDLYSSEDWIKTVQFYSSVFQDLITEGYLSPHSKS 120
            G GRAG+AALFTDNADPRVIGPPRPDLYSS+DWIKTVQF+SSVF+DL+TEGYLSP+SKS
Sbjct: 61  -GAGRAGNAALFTDNADPRVIGPPRPDLYSSKDWIKTVQFFSSVFRDLVTEGYLSPNSKS 120

Query: 121 LCVETPAGADVSALKEIGVSDSVGIFKKALKPLVIKGEGHRIPFEDKTFDFVFSGGGRLD 180
           LCVETPAG DVSALK IGVSDSVGIFKKALKPLVIKGE HRIPF D TFDFVFSGGGRLD
Sbjct: 121 LCVETPAGPDVSALKXIGVSDSVGIFKKALKPLVIKGEAHRIPFGDNTFDFVFSGGGRLD 180

Query: 181 ISRRASDFASEIGRILKPEGFAVVHVSAKDTYSFNSFVDLFNCCKIVKSKDIDVVLPSMP 240
           ISR   +FASEI RILKPEGFAVVHVSAKDTYSFNSFVDLFNCCKIVKS+DIDVVLPSMP
Sbjct: 181 ISRWPYEFASEIARILKPEGFAVVHVSAKDTYSFNSFVDLFNCCKIVKSRDIDVVLPSMP 240

Query: 241 FIREYVLKKEYRILENGLQKLDGDSSKKNCSVPGFKLSLVRNAEPLILEEPLKPWLTLKR 300
            IREYVLKKEYR+LE GL KLDGDS KKNCSVPG+KL+LVRNAEPLILEEPLKPWLTLKR
Sbjct: 241 LIREYVLKKEYRVLEKGLLKLDGDSHKKNCSVPGYKLALVRNAEPLILEEPLKPWLTLKR 300

Query: 301 NIQNIKYLPSMAEISFKNRYVYVDVGARSYGSSIGSWFKKQYPKQNKTFEVYAIEADQTF 360
           NIQ IKYLPSMAEISFKNRYVYVDVGARSYGSSIGSWFKKQYPKQNKTFEVYAIEAD+TF
Sbjct: 301 NIQKIKYLPSMAEISFKNRYVYVDVGARSYGSSIGSWFKKQYPKQNKTFEVYAIEADRTF 360

Query: 361 HEQYRSKKGVTLLPYAAWVRNETLAFEINKDPGQEMEEKGSRGMGRIQPVQSSGHFDGEV 420
           HEQYRSKK VTLLPYAAWVRNETLAFEINKDPGQE EEKG RGMGRIQPVQSSGHFDGEV
Sbjct: 361 HEQYRSKKAVTLLPYAAWVRNETLAFEINKDPGQEKEEKGGRGMGRIQPVQSSGHFDGEV 420

Query: 421 NQIQGFDFANWLKNTVSEKDFVVLKMDVEGTEFELIPRLFETGAICLIDEMFLECHYNRW 480
           NQI+GFDFANWLKNTVSEKDFVVLKMDVEGTEFELIPRLFETGAICLIDEMFLECHYNRW
Sbjct: 421 NQIEGFDFANWLKNTVSEKDFVVLKMDVEGTEFELIPRLFETGAICLIDEMFLECHYNRW 480

Query: 481 QRCCPGQRSTKYEKTYSQCIELFASLRQSGVLVHQWW 518
           QRCCPGQRS+KYEKTYSQCIELF SLRQSGVLVHQWW
Sbjct: 481 QRCCPGQRSSKYEKTYSQCIELFTSLRQSGVLVHQWW 516

BLAST of Spg012974 vs. NCBI nr
Match: KAG6603555.1 (hypothetical protein SDJN03_04164, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 978.8 bits (2529), Expect = 1.8e-281
Identity = 482/517 (93.23%), Postives = 496/517 (95.94%), Query Frame = 0

Query: 1   MGSMSGKFASLRNLLVRVLLFSILVILGRFAYIVTIAGESCSIGDFCFFSLPENFNFMIA 60
           MGS+SGKFASLRNLLVRVLLFSILVILGRFAYIVTIAGESCSIGDFCFFSLPENFNFMIA
Sbjct: 1   MGSVSGKFASLRNLLVRVLLFSILVILGRFAYIVTIAGESCSIGDFCFFSLPENFNFMIA 60

Query: 61  GGGGRAGSAALFTDNADPRVIGPPRPDLYSSEDWIKTVQFYSSVFQDLITEGYLSPHSKS 120
            GGGR GSAAL TDN D RVIGPPRPDLYSSEDWIKTVQFYSSVFQDL+TEGYLS +SK 
Sbjct: 61  -GGGRVGSAALITDNTDSRVIGPPRPDLYSSEDWIKTVQFYSSVFQDLVTEGYLSSNSKV 120

Query: 121 LCVETPAGADVSALKEIGVSDSVGIFKKALKPLVIKGEGHRIPFEDKTFDFVFSGGGRLD 180
           LC+ETPAGADVSALKEIGVSDSVGIFKKALKP VIKGE HRIPFEDKTFDFVFSGGGRLD
Sbjct: 121 LCIETPAGADVSALKEIGVSDSVGIFKKALKPHVIKGEAHRIPFEDKTFDFVFSGGGRLD 180

Query: 181 ISRRASDFASEIGRILKPEGFAVVHVSAKDTYSFNSFVDLFNCCKIVKSKDIDVVLPSMP 240
           IS R  DFASEI RILKPEGFAVVHVSAKDTYSFNSFVDLFNCCKI+KSKDID  LPSMP
Sbjct: 181 ISHRPYDFASEIARILKPEGFAVVHVSAKDTYSFNSFVDLFNCCKILKSKDIDNFLPSMP 240

Query: 241 FIREYVLKKEYRILENGLQKLDGDSSKKNCSVPGFKLSLVRNAEPLILEEPLKPWLTLKR 300
           F REYVLKKE+RILEN LQKLDGDS KKNCSVPG+KL+LVRNAEPLILEEPLKPWLTLKR
Sbjct: 241 FTREYVLKKEFRILENRLQKLDGDSYKKNCSVPGYKLALVRNAEPLILEEPLKPWLTLKR 300

Query: 301 NIQNIKYLPSMAEISFKNRYVYVDVGARSYGSSIGSWFKKQYPKQNKTFEVYAIEADQTF 360
           NIQNIKYLPSMAEISFKNRYVYVDVGARSYGSSIGSWFKKQYPKQNKTFEVYAIEADQTF
Sbjct: 301 NIQNIKYLPSMAEISFKNRYVYVDVGARSYGSSIGSWFKKQYPKQNKTFEVYAIEADQTF 360

Query: 361 HEQYRSKKGVTLLPYAAWVRNETLAFEINKDPGQEMEEKGSRGMGRIQPVQSSGHFDGEV 420
           HEQYRSKK VTLLPYAAWVRNETLAFEINKDPGQEME+KGSRGMGRIQPV+SSGHF+GEV
Sbjct: 361 HEQYRSKKRVTLLPYAAWVRNETLAFEINKDPGQEMEDKGSRGMGRIQPVKSSGHFNGEV 420

Query: 421 NQIQGFDFANWLKNTVSEKDFVVLKMDVEGTEFELIPRLFETGAICLIDEMFLECHYNRW 480
           NQIQGFDFA WLKNTVSEKDFVVLKMDVEGTEFELIPRLFETGAICLIDEMFLECHYNRW
Sbjct: 421 NQIQGFDFATWLKNTVSEKDFVVLKMDVEGTEFELIPRLFETGAICLIDEMFLECHYNRW 480

Query: 481 QRCCPGQRSTKYEKTYSQCIELFASLRQSGVLVHQWW 518
           QRCCPG+RS KYEKTYS+CI+LFASLRQSGVLVHQWW
Sbjct: 481 QRCCPGERSRKYEKTYSECIDLFASLRQSGVLVHQWW 516

BLAST of Spg012974 vs. NCBI nr
Match: XP_023544534.1 (uncharacterized protein LOC111804083 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 977.6 bits (2526), Expect = 4.1e-281
Identity = 481/517 (93.04%), Postives = 497/517 (96.13%), Query Frame = 0

Query: 1   MGSMSGKFASLRNLLVRVLLFSILVILGRFAYIVTIAGESCSIGDFCFFSLPENFNFMIA 60
           MGS+SGKFASLRNLLVRVLLFSILVILGRFAYIVTIAGESCSIGDFCFFSLPENFNFMIA
Sbjct: 1   MGSVSGKFASLRNLLVRVLLFSILVILGRFAYIVTIAGESCSIGDFCFFSLPENFNFMIA 60

Query: 61  GGGGRAGSAALFTDNADPRVIGPPRPDLYSSEDWIKTVQFYSSVFQDLITEGYLSPHSKS 120
             GGRAGSAAL TDN D RVIGPPRPDL+SSEDWIKTVQFYSSVFQDL+TEGYLS +SK+
Sbjct: 61  -SGGRAGSAALLTDNTDSRVIGPPRPDLFSSEDWIKTVQFYSSVFQDLVTEGYLSSNSKA 120

Query: 121 LCVETPAGADVSALKEIGVSDSVGIFKKALKPLVIKGEGHRIPFEDKTFDFVFSGGGRLD 180
           LC+ETPAGADVSALKEIGVSDSVGIFKKALKP VIKGE HRIPFEDKTFDFVFSGGGRLD
Sbjct: 121 LCIETPAGADVSALKEIGVSDSVGIFKKALKPHVIKGEAHRIPFEDKTFDFVFSGGGRLD 180

Query: 181 ISRRASDFASEIGRILKPEGFAVVHVSAKDTYSFNSFVDLFNCCKIVKSKDIDVVLPSMP 240
           IS R  DFASEI RILKPEGFAVVHVSAKDTYSFNSFVDLFNCCKI+KSKDI   LPSMP
Sbjct: 181 ISHRPYDFASEIARILKPEGFAVVHVSAKDTYSFNSFVDLFNCCKILKSKDIHNFLPSMP 240

Query: 241 FIREYVLKKEYRILENGLQKLDGDSSKKNCSVPGFKLSLVRNAEPLILEEPLKPWLTLKR 300
           F REYVLKKE+RILEN LQKLDGDS KKNCSVPG+KL+LVRNAEPLILEEPLKPWLTLKR
Sbjct: 241 FTREYVLKKEFRILENRLQKLDGDSYKKNCSVPGYKLALVRNAEPLILEEPLKPWLTLKR 300

Query: 301 NIQNIKYLPSMAEISFKNRYVYVDVGARSYGSSIGSWFKKQYPKQNKTFEVYAIEADQTF 360
           NIQNIKYLPSMAEISFKNRYVYVDVGARSYGSSIGSWFKKQYPKQNKTFEVYAIEADQTF
Sbjct: 301 NIQNIKYLPSMAEISFKNRYVYVDVGARSYGSSIGSWFKKQYPKQNKTFEVYAIEADQTF 360

Query: 361 HEQYRSKKGVTLLPYAAWVRNETLAFEINKDPGQEMEEKGSRGMGRIQPVQSSGHFDGEV 420
           HEQYRSKK VTLLPYAAWVRNETLAFEINKDPGQEM++KGSRGMGRIQPVQSSGHF+GEV
Sbjct: 361 HEQYRSKKRVTLLPYAAWVRNETLAFEINKDPGQEMDDKGSRGMGRIQPVQSSGHFNGEV 420

Query: 421 NQIQGFDFANWLKNTVSEKDFVVLKMDVEGTEFELIPRLFETGAICLIDEMFLECHYNRW 480
           NQIQGFDFANWLKNTVSEKDFVVLKMDVEGTEFELIPRLFETGAICLIDEMFLECHYNRW
Sbjct: 421 NQIQGFDFANWLKNTVSEKDFVVLKMDVEGTEFELIPRLFETGAICLIDEMFLECHYNRW 480

Query: 481 QRCCPGQRSTKYEKTYSQCIELFASLRQSGVLVHQWW 518
           QRCCPG+RS KYEKTYS+CI+LFASLRQSGVLVHQWW
Sbjct: 481 QRCCPGERSRKYEKTYSECIDLFASLRQSGVLVHQWW 516

BLAST of Spg012974 vs. ExPASy TrEMBL
Match: A0A6J1IP93 (uncharacterized protein LOC111478211 OS=Cucurbita maxima OX=3661 GN=LOC111478211 PE=4 SV=1)

HSP 1 Score: 987.6 bits (2552), Expect = 1.9e-284
Identity = 486/517 (94.00%), Postives = 500/517 (96.71%), Query Frame = 0

Query: 1   MGSMSGKFASLRNLLVRVLLFSILVILGRFAYIVTIAGESCSIGDFCFFSLPENFNFMIA 60
           MGS+SGKFASLRNLLVRVLLFSILVILGRFAYIVTIAGESC IGDFCFFSLPENFNFMIA
Sbjct: 1   MGSVSGKFASLRNLLVRVLLFSILVILGRFAYIVTIAGESCRIGDFCFFSLPENFNFMIA 60

Query: 61  GGGGRAGSAALFTDNADPRVIGPPRPDLYSSEDWIKTVQFYSSVFQDLITEGYLSPHSKS 120
            GGGRAGSAALFTDN D RVIGPPRPDLYSSEDWIKTVQFYSSVFQDL+TEGYLS +SK+
Sbjct: 61  -GGGRAGSAALFTDNTDSRVIGPPRPDLYSSEDWIKTVQFYSSVFQDLVTEGYLSSNSKA 120

Query: 121 LCVETPAGADVSALKEIGVSDSVGIFKKALKPLVIKGEGHRIPFEDKTFDFVFSGGGRLD 180
           LC+ETPAGADVSALKEIGVSDSVGIFKKALKP VIKGE HRIPFEDKTFDFVFSGGGRLD
Sbjct: 121 LCIETPAGADVSALKEIGVSDSVGIFKKALKPHVIKGEAHRIPFEDKTFDFVFSGGGRLD 180

Query: 181 ISRRASDFASEIGRILKPEGFAVVHVSAKDTYSFNSFVDLFNCCKIVKSKDIDVVLPSMP 240
           IS+R  DFASEI RILKPEGFAVVHVSAKDTYSFNSFVDLFNCCKI+KSKDID  LPSMP
Sbjct: 181 ISQRPYDFASEIARILKPEGFAVVHVSAKDTYSFNSFVDLFNCCKILKSKDIDNFLPSMP 240

Query: 241 FIREYVLKKEYRILENGLQKLDGDSSKKNCSVPGFKLSLVRNAEPLILEEPLKPWLTLKR 300
           F REYVLKKEYRILEN LQKLDGDS KKNCSVPG+KLSLVRNAEPLILEEPLKPWLTLKR
Sbjct: 241 FTREYVLKKEYRILENRLQKLDGDSYKKNCSVPGYKLSLVRNAEPLILEEPLKPWLTLKR 300

Query: 301 NIQNIKYLPSMAEISFKNRYVYVDVGARSYGSSIGSWFKKQYPKQNKTFEVYAIEADQTF 360
           NIQNIKYLPSMAEISFKN+YVYVDVGARSYGSSIGSWFKKQYPKQNKTFEVYAIEADQTF
Sbjct: 301 NIQNIKYLPSMAEISFKNKYVYVDVGARSYGSSIGSWFKKQYPKQNKTFEVYAIEADQTF 360

Query: 361 HEQYRSKKGVTLLPYAAWVRNETLAFEINKDPGQEMEEKGSRGMGRIQPVQSSGHFDGEV 420
           HEQYRSKK VTLLPYAAWVRNETLAFEINKDPGQEME+KGSRGMGRIQPVQSSGHF+GEV
Sbjct: 361 HEQYRSKKRVTLLPYAAWVRNETLAFEINKDPGQEMEDKGSRGMGRIQPVQSSGHFNGEV 420

Query: 421 NQIQGFDFANWLKNTVSEKDFVVLKMDVEGTEFELIPRLFETGAICLIDEMFLECHYNRW 480
           NQIQGFDFANWLKNTVSEKDFVVLKMDVEGTEFELIPRLFETGAICLIDEMFLECHYNRW
Sbjct: 421 NQIQGFDFANWLKNTVSEKDFVVLKMDVEGTEFELIPRLFETGAICLIDEMFLECHYNRW 480

Query: 481 QRCCPGQRSTKYEKTYSQCIELFASLRQSGVLVHQWW 518
           QRCCPG+RS KYEKTYS+CI+LFASLRQSGVLVHQWW
Sbjct: 481 QRCCPGERSRKYEKTYSECIDLFASLRQSGVLVHQWW 516

BLAST of Spg012974 vs. ExPASy TrEMBL
Match: A0A6J1CQP9 (uncharacterized protein LOC111013875 OS=Momordica charantia OX=3673 GN=LOC111013875 PE=4 SV=1)

HSP 1 Score: 980.3 bits (2533), Expect = 3.1e-282
Identity = 480/517 (92.84%), Postives = 496/517 (95.94%), Query Frame = 0

Query: 1   MGSMSGKFASLRNLLVRVLLFSILVILGRFAYIVTIAGESCSIGDFCFFSLPENFNFMIA 60
           MGSMSGKFASLRNLLVRVLLFSIL+I+GRFAYIVTI GESCSIGDFCFFSLPENFNFMIA
Sbjct: 1   MGSMSGKFASLRNLLVRVLLFSILIIIGRFAYIVTITGESCSIGDFCFFSLPENFNFMIA 60

Query: 61  GGGGRAGSAALFTDNADPRVIGPPRPDLYSSEDWIKTVQFYSSVFQDLITEGYLSPHSKS 120
            G GRAG+AALFTDNADPRVIGPPRPDLYSS+DWIKTVQF+SSVF+DL+TEGYLSP+SKS
Sbjct: 61  -GAGRAGNAALFTDNADPRVIGPPRPDLYSSKDWIKTVQFFSSVFRDLVTEGYLSPNSKS 120

Query: 121 LCVETPAGADVSALKEIGVSDSVGIFKKALKPLVIKGEGHRIPFEDKTFDFVFSGGGRLD 180
           LCVETPAG DVSALK IGVSDSVGIFKKALKPLVIKGE HRIPF D TFDFVFSGGGRLD
Sbjct: 121 LCVETPAGPDVSALKXIGVSDSVGIFKKALKPLVIKGEAHRIPFGDNTFDFVFSGGGRLD 180

Query: 181 ISRRASDFASEIGRILKPEGFAVVHVSAKDTYSFNSFVDLFNCCKIVKSKDIDVVLPSMP 240
           ISR   +FASEI RILKPEGFAVVHVSAKDTYSFNSFVDLFNCCKIVKS+DIDVVLPSMP
Sbjct: 181 ISRWPYEFASEIARILKPEGFAVVHVSAKDTYSFNSFVDLFNCCKIVKSRDIDVVLPSMP 240

Query: 241 FIREYVLKKEYRILENGLQKLDGDSSKKNCSVPGFKLSLVRNAEPLILEEPLKPWLTLKR 300
            IREYVLKKEYR+LE GL KLDGDS KKNCSVPG+KL+LVRNAEPLILEEPLKPWLTLKR
Sbjct: 241 LIREYVLKKEYRVLEKGLLKLDGDSHKKNCSVPGYKLALVRNAEPLILEEPLKPWLTLKR 300

Query: 301 NIQNIKYLPSMAEISFKNRYVYVDVGARSYGSSIGSWFKKQYPKQNKTFEVYAIEADQTF 360
           NIQ IKYLPSMAEISFKNRYVYVDVGARSYGSSIGSWFKKQYPKQNKTFEVYAIEAD+TF
Sbjct: 301 NIQKIKYLPSMAEISFKNRYVYVDVGARSYGSSIGSWFKKQYPKQNKTFEVYAIEADRTF 360

Query: 361 HEQYRSKKGVTLLPYAAWVRNETLAFEINKDPGQEMEEKGSRGMGRIQPVQSSGHFDGEV 420
           HEQYRSKK VTLLPYAAWVRNETLAFEINKDPGQE EEKG RGMGRIQPVQSSGHFDGEV
Sbjct: 361 HEQYRSKKAVTLLPYAAWVRNETLAFEINKDPGQEKEEKGGRGMGRIQPVQSSGHFDGEV 420

Query: 421 NQIQGFDFANWLKNTVSEKDFVVLKMDVEGTEFELIPRLFETGAICLIDEMFLECHYNRW 480
           NQI+GFDFANWLKNTVSEKDFVVLKMDVEGTEFELIPRLFETGAICLIDEMFLECHYNRW
Sbjct: 421 NQIEGFDFANWLKNTVSEKDFVVLKMDVEGTEFELIPRLFETGAICLIDEMFLECHYNRW 480

Query: 481 QRCCPGQRSTKYEKTYSQCIELFASLRQSGVLVHQWW 518
           QRCCPGQRS+KYEKTYSQCIELF SLRQSGVLVHQWW
Sbjct: 481 QRCCPGQRSSKYEKTYSQCIELFTSLRQSGVLVHQWW 516

BLAST of Spg012974 vs. ExPASy TrEMBL
Match: A0A6J1GE05 (uncharacterized protein LOC111453090 OS=Cucurbita moschata OX=3662 GN=LOC111453090 PE=4 SV=1)

HSP 1 Score: 977.2 bits (2525), Expect = 2.6e-281
Identity = 481/517 (93.04%), Postives = 495/517 (95.74%), Query Frame = 0

Query: 1   MGSMSGKFASLRNLLVRVLLFSILVILGRFAYIVTIAGESCSIGDFCFFSLPENFNFMIA 60
           MGS+SGKFASLRNLLVRVLLFSILVILGRFAYIVTIAGESCSIGDFCFFSLPENFNFMIA
Sbjct: 1   MGSVSGKFASLRNLLVRVLLFSILVILGRFAYIVTIAGESCSIGDFCFFSLPENFNFMIA 60

Query: 61  GGGGRAGSAALFTDNADPRVIGPPRPDLYSSEDWIKTVQFYSSVFQDLITEGYLSPHSKS 120
            GGGR GSAAL TDN D RVIGPPRPDLYSSEDWIKTVQFYSSVFQDL+TEGYLS +SK 
Sbjct: 61  -GGGRVGSAALITDNTDSRVIGPPRPDLYSSEDWIKTVQFYSSVFQDLVTEGYLSSNSKV 120

Query: 121 LCVETPAGADVSALKEIGVSDSVGIFKKALKPLVIKGEGHRIPFEDKTFDFVFSGGGRLD 180
           LC+ETPAGADVSALKEIGVSDSVGIFKKALKP VIKGE HRIPFEDKTFDFVFSGGGRLD
Sbjct: 121 LCIETPAGADVSALKEIGVSDSVGIFKKALKPHVIKGEAHRIPFEDKTFDFVFSGGGRLD 180

Query: 181 ISRRASDFASEIGRILKPEGFAVVHVSAKDTYSFNSFVDLFNCCKIVKSKDIDVVLPSMP 240
           IS R  DFASEI RILKPEGFAVVHVSAKDTYSFNSFVDLFNCCKI+KSKDID  LPSMP
Sbjct: 181 ISHRPYDFASEIARILKPEGFAVVHVSAKDTYSFNSFVDLFNCCKILKSKDIDNFLPSMP 240

Query: 241 FIREYVLKKEYRILENGLQKLDGDSSKKNCSVPGFKLSLVRNAEPLILEEPLKPWLTLKR 300
           F REYVLKKE+RILEN LQKLDGDS KKNCSVPG+KL+LVRNAEPLILEEPLKPWLTLKR
Sbjct: 241 FTREYVLKKEFRILENRLQKLDGDSYKKNCSVPGYKLALVRNAEPLILEEPLKPWLTLKR 300

Query: 301 NIQNIKYLPSMAEISFKNRYVYVDVGARSYGSSIGSWFKKQYPKQNKTFEVYAIEADQTF 360
           NIQNIKYLPSMAEISFKNRYVYVDVGARSYGSSIGSWFKKQYPKQNKTFEVYAIEADQTF
Sbjct: 301 NIQNIKYLPSMAEISFKNRYVYVDVGARSYGSSIGSWFKKQYPKQNKTFEVYAIEADQTF 360

Query: 361 HEQYRSKKGVTLLPYAAWVRNETLAFEINKDPGQEMEEKGSRGMGRIQPVQSSGHFDGEV 420
           HEQYRSKK VTLLPYAAWVRNETLAFEINKDPGQEME+KG RGMGRIQPV+SSGHF+GEV
Sbjct: 361 HEQYRSKKRVTLLPYAAWVRNETLAFEINKDPGQEMEDKGGRGMGRIQPVKSSGHFNGEV 420

Query: 421 NQIQGFDFANWLKNTVSEKDFVVLKMDVEGTEFELIPRLFETGAICLIDEMFLECHYNRW 480
           NQIQGFDFA WLKNTVSEKDFVVLKMDVEGTEFELIPRLFETGAICLIDEMFLECHYNRW
Sbjct: 421 NQIQGFDFATWLKNTVSEKDFVVLKMDVEGTEFELIPRLFETGAICLIDEMFLECHYNRW 480

Query: 481 QRCCPGQRSTKYEKTYSQCIELFASLRQSGVLVHQWW 518
           QRCCPG+RS KYEKTYS+CI+LFASLRQSGVLVHQWW
Sbjct: 481 QRCCPGERSRKYEKTYSECIDLFASLRQSGVLVHQWW 516

BLAST of Spg012974 vs. ExPASy TrEMBL
Match: A0A0A0L2S2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G314480 PE=4 SV=1)

HSP 1 Score: 976.9 bits (2524), Expect = 3.4e-281
Identity = 481/520 (92.50%), Postives = 497/520 (95.58%), Query Frame = 0

Query: 1   MGSMSGKFASLRNLLVRVLLFSILVILGRFAYIVTIAGESCSIGDFCFFSLPENFNFMIA 60
           MGSM GKFASLRNLLVRVLLFSILVI+GRFAY+VTIAGESCSIGDFCFFSLPE FNFMI 
Sbjct: 1   MGSMLGKFASLRNLLVRVLLFSILVIIGRFAYVVTIAGESCSIGDFCFFSLPETFNFMIP 60

Query: 61  GGGGRAGSAALFTDNADPRVIGPPRPDLYSSEDWIKTVQFYSSVFQDLITEGYLSPHSKS 120
           GGGGR GSAALFTDNADPRVIGPPRPDLYSSEDWIKTVQFYSSVFQDLITEGYLSP+SKS
Sbjct: 61  GGGGRTGSAALFTDNADPRVIGPPRPDLYSSEDWIKTVQFYSSVFQDLITEGYLSPNSKS 120

Query: 121 LCVETPAGADVSALKEIGVSDSVGIFKKALKPLVIKGEGHRIPFEDKTFDFVFSGGGRLD 180
           LC+ETPAGADVSALK+IGVS+SVGIFKKALKPLVIKGE HRIPFED TFDFVFSGG RLD
Sbjct: 121 LCIETPAGADVSALKDIGVSNSVGIFKKALKPLVIKGEAHRIPFEDNTFDFVFSGGSRLD 180

Query: 181 ISRRASDFASEIGRILKPEGFAVVHVSAKDTYSFNSFVDLFNCCKIVKSKDIDVVLPSMP 240
           ISRR  DFASEI RILKPEGFAVVHVSAKDTYSFNSFVDLFNCCKI+K+KDID+VLPSMP
Sbjct: 181 ISRRPHDFASEIARILKPEGFAVVHVSAKDTYSFNSFVDLFNCCKILKTKDIDIVLPSMP 240

Query: 241 FIREYVLKKEYRILENGLQKL--DGDSSKKNCSVPGFKLSLVRNAEPLILEEPLKPWLTL 300
             REYVLKKEYRILENGL KL  DG S KKNCSVPG+KL+L RNAEPLILEEPLKPWLTL
Sbjct: 241 STREYVLKKEYRILENGLLKLNEDGVSYKKNCSVPGYKLALFRNAEPLILEEPLKPWLTL 300

Query: 301 KRNIQNIKYLPSMAEISFKNRYVYVDVGARSYGSSIGSWFKKQYPKQNKTFEVYAIEADQ 360
           KRNIQN+KYLPSMAEISFKNRYVYVDVGARSYGSSIGSWFKKQYPKQNKTFEVYAIEADQ
Sbjct: 301 KRNIQNVKYLPSMAEISFKNRYVYVDVGARSYGSSIGSWFKKQYPKQNKTFEVYAIEADQ 360

Query: 361 TFHEQYRSKKGVTLLPYAAWVRNETLAFEINKDPGQEMEEKG-SRGMGRIQPVQSSGHFD 420
           TFHEQY+SKKGVTLLPYAAWVRNETLAFEINKDPGQ  E+KG SRGMGRIQPVQS G FD
Sbjct: 361 TFHEQYKSKKGVTLLPYAAWVRNETLAFEINKDPGQGKEDKGASRGMGRIQPVQSLGQFD 420

Query: 421 GEVNQIQGFDFANWLKNTVSEKDFVVLKMDVEGTEFELIPRLFETGAICLIDEMFLECHY 480
           GEVNQIQGFDFANWLKNTVSEKDFVVLKMDVEGTEFELIPRLFETGAICLIDEMFLECHY
Sbjct: 421 GEVNQIQGFDFANWLKNTVSEKDFVVLKMDVEGTEFELIPRLFETGAICLIDEMFLECHY 480

Query: 481 NRWQRCCPGQRSTKYEKTYSQCIELFASLRQSGVLVHQWW 518
           NRWQRCCPGQRSTKYEKTYS+CI+LFASLRQSGVLVHQWW
Sbjct: 481 NRWQRCCPGQRSTKYEKTYSECIDLFASLRQSGVLVHQWW 520

BLAST of Spg012974 vs. ExPASy TrEMBL
Match: A0A1S3CL16 (uncharacterized protein LOC103502172 OS=Cucumis melo OX=3656 GN=LOC103502172 PE=4 SV=1)

HSP 1 Score: 976.1 bits (2522), Expect = 5.8e-281
Identity = 483/520 (92.88%), Postives = 496/520 (95.38%), Query Frame = 0

Query: 1   MGSMSGKFASLRNLLVRVLLFSILVILGRFAYIVTIAGESCSIGDFCFFSLPENFNFMIA 60
           MGSM GKFASLRNLLVRVLLFSILVI+GRFAY+VTIAGESCSIGDFCFFSLPE FNFMI 
Sbjct: 1   MGSMLGKFASLRNLLVRVLLFSILVIIGRFAYVVTIAGESCSIGDFCFFSLPETFNFMIP 60

Query: 61  GGGGRAGSAALFTDNADPRVIGPPRPDLYSSEDWIKTVQFYSSVFQDLITEGYLSPHSKS 120
           G GGR GSAALFTDN DPRVIGPPRPDLYSSEDWIKTVQFYSSVFQDLITEGYLSP+SKS
Sbjct: 61  GSGGRTGSAALFTDNTDPRVIGPPRPDLYSSEDWIKTVQFYSSVFQDLITEGYLSPNSKS 120

Query: 121 LCVETPAGADVSALKEIGVSDSVGIFKKALKPLVIKGEGHRIPFEDKTFDFVFSGGGRLD 180
           LC+ETPAGADVSALKEIGVS+SVGIFKKALKPLVIKGE HRIPFED TFDFVFSGG RLD
Sbjct: 121 LCIETPAGADVSALKEIGVSNSVGIFKKALKPLVIKGEAHRIPFEDNTFDFVFSGGSRLD 180

Query: 181 ISRRASDFASEIGRILKPEGFAVVHVSAKDTYSFNSFVDLFNCCKIVKSKDIDVVLPSMP 240
           ISRR  DFASEI RILKPEGFAVVHVSAKDTYSFNSFVDLFN CKI+KSKDID+VLPSMP
Sbjct: 181 ISRRPHDFASEIARILKPEGFAVVHVSAKDTYSFNSFVDLFNSCKILKSKDIDIVLPSMP 240

Query: 241 FIREYVLKKEYRILENGLQKL--DGDSSKKNCSVPGFKLSLVRNAEPLILEEPLKPWLTL 300
             REYVLKKEYRILENGL KL  DG S KKNCSVPG+KL+L RNAEPLILEEPLKPWLTL
Sbjct: 241 STREYVLKKEYRILENGLLKLNEDGVSYKKNCSVPGYKLALFRNAEPLILEEPLKPWLTL 300

Query: 301 KRNIQNIKYLPSMAEISFKNRYVYVDVGARSYGSSIGSWFKKQYPKQNKTFEVYAIEADQ 360
           KRNIQNIKYLPSMAEISFKNRYVYVDVGARSYGSSIGSWFKKQYPKQNKTFEVYAIEADQ
Sbjct: 301 KRNIQNIKYLPSMAEISFKNRYVYVDVGARSYGSSIGSWFKKQYPKQNKTFEVYAIEADQ 360

Query: 361 TFHEQYRSKKGVTLLPYAAWVRNETLAFEINKDPGQEMEEK-GSRGMGRIQPVQSSGHFD 420
           TFHEQY+SKKGVTLLPYAAWVRNETLAFEINKDPGQE E+K GSRGMGRIQPVQSSG FD
Sbjct: 361 TFHEQYKSKKGVTLLPYAAWVRNETLAFEINKDPGQEKEDKGGSRGMGRIQPVQSSGQFD 420

Query: 421 GEVNQIQGFDFANWLKNTVSEKDFVVLKMDVEGTEFELIPRLFETGAICLIDEMFLECHY 480
           GEVNQIQGFDFA+WLKNTVSEKDFVVLKMDVEGTEFELIPRLFETGAICLIDEMFLECHY
Sbjct: 421 GEVNQIQGFDFADWLKNTVSEKDFVVLKMDVEGTEFELIPRLFETGAICLIDEMFLECHY 480

Query: 481 NRWQRCCPGQRSTKYEKTYSQCIELFASLRQSGVLVHQWW 518
           NRWQRCCPGQRSTKYEKTYSQCI+LFASLRQSGVLVHQWW
Sbjct: 481 NRWQRCCPGQRSTKYEKTYSQCIDLFASLRQSGVLVHQWW 520

BLAST of Spg012974 vs. TAIR 10
Match: AT5G01710.1 (methyltransferases )

HSP 1 Score: 719.2 bits (1855), Expect = 2.4e-207
Identity = 354/520 (68.08%), Postives = 418/520 (80.38%), Query Frame = 0

Query: 1   MGSMSGKFASLRNLLVRVLLFSILVILGRFAYIVTIAGESCSIGDFCFFSLPENFNFMIA 60
           M +  GK + LRN++VRVLLF +L+I+ RFAY+VTI GESC+ GDFCFFSLPEN NF+I+
Sbjct: 1   METSMGKPSFLRNIMVRVLLFGVLIIVVRFAYVVTITGESCNRGDFCFFSLPENLNFVIS 60

Query: 61  GGGGRAGSAALFTDNADPRVIGPPRPDLYSSEDWIKTVQFYSSVFQDLITEGYLSPHSKS 120
           G    +GS+A+     D      P  DLY++ DWIK+VQFYSS+FQDLI +GYLSP SK+
Sbjct: 61  GAA--SGSSAI-----DAIRSTSPGDDLYTTRDWIKSVQFYSSIFQDLIADGYLSPESKT 120

Query: 121 LCVETPAGADVSALKEIGVSDSVGIFKKALKPLVIKGEGHRIPFEDKTFDFVFSGGGRLD 180
           LCVET  G +V +L+EIGV +SVGI KKA +PLV++GEGH IPFED  FDFVFSGG RL 
Sbjct: 121 LCVETAIGQEVHSLREIGVKNSVGISKKASRPLVVRGEGHAIPFEDNAFDFVFSGGDRLG 180

Query: 181 ISRRASDFASEIGRILKPEGFAVVHVSAKDTYSFNSFVDLFNCCKIVKSKDIDVVLPSMP 240
            S +  +FA EI R LKPEGFAVVHV A DTYSFNSF+DLFN C++VK +DID   PSMP
Sbjct: 181 KSLKQLEFADEITRTLKPEGFAVVHVGATDTYSFNSFLDLFNSCRLVKMRDIDGFDPSMP 240

Query: 241 FIREYVLKKEYRILENGLQKLDGDSSKKNCSVPGFKLSLVRNAEPLILEEPLKPWLTLKR 300
            IRE+V++K   I   G     G  S   C +PG+K  L+R+AEPLI EEPLKPW+TLKR
Sbjct: 241 HIREFVIQKYSEIDGGGHHHRGGGDSGGKCWIPGYKRDLIRDAEPLIQEEPLKPWITLKR 300

Query: 301 NIQNIKYLPSMAEISFKNRYVYVDVGARSYGSSIGSWFKKQYPKQNKTFEVYAIEADQTF 360
           NI+NIKY+PSM +I FK+RYVYVDVGARSYGSSIGSWFKK+YPKQNKTF+V+AIEAD+ F
Sbjct: 301 NIKNIKYVPSMVDIRFKSRYVYVDVGARSYGSSIGSWFKKEYPKQNKTFDVFAIEADKAF 360

Query: 361 HEQYRSKKGVTLLPYAAWVRNETLAFEINKDPGQEMEEKG-SRGMGRIQPVQSSGHFD-- 420
           HE+Y+ KK V LLPYAAWVRNETL+FEIN DPG+E+E K   RGMGRIQPV+ S   D  
Sbjct: 361 HEEYKIKKKVNLLPYAAWVRNETLSFEINHDPGKEVEAKAKGRGMGRIQPVKKSDSSDLA 420

Query: 421 GEVNQIQGFDFANWLKNTVSEKDFVVLKMDVEGTEFELIPRLFETGAICLIDEMFLECHY 480
           GEVN IQGFDFA+WLK +V E+DFVV+KMDVEGTEF+LIPRL +TGAICLIDE+FLECHY
Sbjct: 421 GEVNLIQGFDFADWLKKSVRERDFVVMKMDVEGTEFDLIPRLIKTGAICLIDELFLECHY 480

Query: 481 NRWQRCCPGQRSTKYEKTYSQCIELFASLRQSGVLVHQWW 518
           NRWQRCCPGQRS KY KTY+QC+ELF SLRQ GVLVHQWW
Sbjct: 481 NRWQRCCPGQRSQKYNKTYNQCLELFNSLRQRGVLVHQWW 513

BLAST of Spg012974 vs. TAIR 10
Match: AT3G53400.1 (BEST Arabidopsis thaliana protein match is: conserved peptide upstream open reading frame 47 (TAIR:AT5G03190.1); Has 285 Blast hits to 285 proteins in 23 species: Archae - 0; Bacteria - 0; Metazoa - 1; Fungi - 0; Plants - 279; Viruses - 0; Other Eukaryotes - 5 (source: NCBI BLink). )

HSP 1 Score: 142.5 bits (358), Expect = 9.4e-34
Identity = 115/424 (27.12%), Postives = 175/424 (41.27%), Query Frame = 0

Query: 102 SSVFQDLITEGYLSPHSKSLCVETPAGADVSALKEIGVSDSVGIFKKALKPLVIKGEGHR 161
           + V ++L     LS  SK+LC+   + + V A+   G+SD    +   +     +     
Sbjct: 103 TQVVRELTGMNLLSNDSKALCIGRRSVSAVLAMNRQGISDVSVAYMPPVFAFKHRKFTSE 162

Query: 162 IPFEDKTFDFVFSGGGRLDISRRA--SDFASEIGRILKPEGFAVVHVSAKDTYSFNSFVD 221
           + +ED +F FVFS    +D+   A  +    EI RILKP G   + V        N  V 
Sbjct: 163 LHYEDASFGFVFS----MDLESVAVPASLVYEIERILKPGGTGAMLVGTTSGSDSNELVR 222

Query: 222 LFNCCKIVKSKDIDVVLPSMPFIREYVLKKEYRILENGLQKLDGDSS----KKNCSVPGF 281
                       +  +L +   +    L K+  +    + K DG+ S    + +   P  
Sbjct: 223 --------SVSPVSSLLKNSSVVHVASLGKQVLV----VFKRDGEDSFRLDQTHHDFPAD 282

Query: 282 KLSLVRNAEPLILEEPLKPWLTLKRN--IQNIKYLPSMAEISFKNRYVYVDVGARSYGSS 341
             S++ N   + L EPL   L  KR+   + I YLP   ++S + R VY+D+GA  +  +
Sbjct: 283 CSSVLNNRPYIGLLEPL---LDEKRSDFERRIHYLPEFIDLSSRKRLVYIDIGAVDHVKA 342

Query: 342 IGSWFKKQYPKQNKTFEVYAIEADQTFHEQYRSKKGVTLLPYAAWVRNETLAFEINKDPG 401
             +WF   YP   K F  Y +  + +    Y    GVT + +      +T          
Sbjct: 343 RSNWFFPSYPIDRKAFNSYFVHHNTSILTSYVKSPGVTFIYHPGLAATKT---------- 402

Query: 402 QEMEEKGSRGMGRIQPVQSSGHFDGEVNQIQGFDFANWLKNTVSEKDFVVLKMDVEGTEF 461
                           + ++G  +    +   FDF  W K T S  DFVVLKM+   TE 
Sbjct: 403 ---------------TIANTGDHEEPFVEDDSFDFLAWFKETASFADFVVLKMNTSDTEL 462

Query: 462 ELIPRLFETGAICLIDEMFLECHYNRWQRCCPGQRSTKYEKTYSQCIELFASLRQSGVLV 518
           + +  L +TGAIC +DE+FL C                    YS C  +  SLR SGV V
Sbjct: 463 KFLSELIKTGAICSVDELFLHC------------------TGYSDCTGIIKSLRNSGVFV 464

BLAST of Spg012974 vs. TAIR 10
Match: AT5G03190.1 (conserved peptide upstream open reading frame 47 )

HSP 1 Score: 139.0 bits (349), Expect = 1.0e-32
Identity = 118/432 (27.31%), Postives = 183/432 (42.36%), Query Frame = 0

Query: 95  IKTVQFYSSVFQDLITE----GYLSPHSKSLCVETPAGADVSALKEIGVSDSVGIFKKAL 154
           I+T ++   V  DL+ E      L   +K LC+   + + VS  KE+G S   G+ K  L
Sbjct: 79  IETEKYPQVVIADLVDELMGLKLLHYDAKILCIGQGSDSAVSGFKEMGFSVVQGVPKHPL 138

Query: 155 KPLVIKGEGHRIPFE-DKTFDFVFSGGGRLDISRRASD--FASEIGRILKPEGFAVVHVS 214
                +   + +    DK+FDFV  G    D+   AS      E+ R+LKP G   V VS
Sbjct: 139 FSFFSRKHVNELELSGDKSFDFVLCG----DVDHVASPALLVLEMERVLKPGGTGAVLVS 198

Query: 215 AKDTYSFNSFVDLFNCCKIVKSKDIDVVLPSMPFIREYVLKKEYRILENGLQKLDGDSSK 274
                   S        +IV+  ++D    ++   +  V +  Y I   G  +L  D   
Sbjct: 199 TNANRLVKSVTSGLKQSEIVRVNNLDKF--TVIVFKRNVTETAYCI---GKSQLPRDCKS 258

Query: 275 KNCSVPGFKLSLVRNAEPLILEEPLKPWLTLKRNIQNIKYLPSMAEISFKNRYVYVDVGA 334
            + + P          EPL+ ++P           +++ YLP   ++S K   VY+D+GA
Sbjct: 259 VDTNRP-----YTEFMEPLLEQKPAD-------FPKSVAYLPKFLDLSLKKSLVYIDIGA 318

Query: 335 RSY--GSSIGSWFKKQYPKQNKTFEVYAIEADQTFHEQYRSKKGVTLLPYAAWVRNETLA 394
             +   +   +WF   YP  +K F VY ++ + +    Y  K GVT + +          
Sbjct: 319 AEHMDANLTPNWFFPLYPLDSKAFNVYFVDHNTSVMLSYVKKPGVTFVYH---------- 378

Query: 395 FEINKDPGQEMEEKGSRGMGRIQPVQSSGHFDGEVNQIQGFDFANWLKNTVSEKDFVVLK 454
                    ++ E  S G  +I P++    F  +    + FDF  W + T    DFVVLK
Sbjct: 379 --------PDLAENNSTGK-KITPLEQLEPFPED----ERFDFLAWFEETAKYADFVVLK 438

Query: 455 MDVEGTEFELIPRLFETGAICLIDEMFLECHYNRWQRCCPGQRSTKYEKTYSQCIELFAS 514
           M+    E + +  L ETG IC +DE+FL C  ++                 S CI +  +
Sbjct: 439 MNTNQVEMKFLTVLLETGVICYVDELFLRCSNHK-----------------SDCINMLQT 449

Query: 515 LRQSGVLVHQWW 518
           LR  GV VHQWW
Sbjct: 499 LRARGVFVHQWW 449

BLAST of Spg012974 vs. TAIR 10
Match: AT5G03190.2 (conserved peptide upstream open reading frame 47 )

HSP 1 Score: 139.0 bits (349), Expect = 1.0e-32
Identity = 118/432 (27.31%), Postives = 183/432 (42.36%), Query Frame = 0

Query: 95  IKTVQFYSSVFQDLITE----GYLSPHSKSLCVETPAGADVSALKEIGVSDSVGIFKKAL 154
           I+T ++   V  DL+ E      L   +K LC+   + + VS  KE+G S   G+ K  L
Sbjct: 103 IETEKYPQVVIADLVDELMGLKLLHYDAKILCIGQGSDSAVSGFKEMGFSVVQGVPKHPL 162

Query: 155 KPLVIKGEGHRIPFE-DKTFDFVFSGGGRLDISRRASD--FASEIGRILKPEGFAVVHVS 214
                +   + +    DK+FDFV  G    D+   AS      E+ R+LKP G   V VS
Sbjct: 163 FSFFSRKHVNELELSGDKSFDFVLCG----DVDHVASPALLVLEMERVLKPGGTGAVLVS 222

Query: 215 AKDTYSFNSFVDLFNCCKIVKSKDIDVVLPSMPFIREYVLKKEYRILENGLQKLDGDSSK 274
                   S        +IV+  ++D    ++   +  V +  Y I   G  +L  D   
Sbjct: 223 TNANRLVKSVTSGLKQSEIVRVNNLDKF--TVIVFKRNVTETAYCI---GKSQLPRDCKS 282

Query: 275 KNCSVPGFKLSLVRNAEPLILEEPLKPWLTLKRNIQNIKYLPSMAEISFKNRYVYVDVGA 334
            + + P          EPL+ ++P           +++ YLP   ++S K   VY+D+GA
Sbjct: 283 VDTNRP-----YTEFMEPLLEQKPAD-------FPKSVAYLPKFLDLSLKKSLVYIDIGA 342

Query: 335 RSY--GSSIGSWFKKQYPKQNKTFEVYAIEADQTFHEQYRSKKGVTLLPYAAWVRNETLA 394
             +   +   +WF   YP  +K F VY ++ + +    Y  K GVT + +          
Sbjct: 343 AEHMDANLTPNWFFPLYPLDSKAFNVYFVDHNTSVMLSYVKKPGVTFVYH---------- 402

Query: 395 FEINKDPGQEMEEKGSRGMGRIQPVQSSGHFDGEVNQIQGFDFANWLKNTVSEKDFVVLK 454
                    ++ E  S G  +I P++    F  +    + FDF  W + T    DFVVLK
Sbjct: 403 --------PDLAENNSTGK-KITPLEQLEPFPED----ERFDFLAWFEETAKYADFVVLK 462

Query: 455 MDVEGTEFELIPRLFETGAICLIDEMFLECHYNRWQRCCPGQRSTKYEKTYSQCIELFAS 514
           M+    E + +  L ETG IC +DE+FL C  ++                 S CI +  +
Sbjct: 463 MNTNQVEMKFLTVLLETGVICYVDELFLRCSNHK-----------------SDCINMLQT 473

Query: 515 LRQSGVLVHQWW 518
           LR  GV VHQWW
Sbjct: 523 LRARGVFVHQWW 473

BLAST of Spg012974 vs. TAIR 10
Match: AT1G24480.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 112.5 bits (280), Expect = 1.0e-24
Identity = 61/164 (37.20%), Postives = 90/164 (54.88%), Query Frame = 0

Query: 79  RVIGPPRPDLYSSEDWIKTVQFYSSVFQDLITEGYLSPHSKSLCVETPAGADVSALKEIG 138
           + + P    ++ + DW + ++ +S  FQDL  +G LS  SK LC+    G +V ALK +G
Sbjct: 56  KTLNPRLRTIWMTRDWDRKIKVFSRFFQDLKRQGLLSKDSKCLCLGARVGQEVEALKRVG 115

Query: 139 VSDSVGIFKKALKPLVIKGEGHRIPFEDKTFDFVFSGGGRLDISRRASDFASEIGRILKP 198
           V+DSVG+      PLV+KG+ H  PF+D+TFDF FS     D +     F  EI R L+P
Sbjct: 116 VNDSVGMDLVPYPPLVVKGDFHHQPFDDETFDFEFS--NVFDHALYPDKFVGEIERTLRP 175

Query: 199 EGFAVVHV---------SAKDTYSFNSFVDLFNCCKIVKSKDID 234
            G  V+HV         SA D +S  + V LF   ++V  +++D
Sbjct: 176 GGLCVLHVALSTRSDKYSANDLFSVEALVKLFRQSEVVHVRNVD 217