Spg012973 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg012973 vs. NCBI nr
Match: XP_022156263.1 (spermidine hydroxycinnamoyl transferase-like [Momordica charantia])

HSP 1 Score: 582.4 bits (1500), Expect = 3.5e-162
Identity = 298/473 (63.00%), Postives = 353/473 (74.63%), Query Frame = 0

Query: 1   MASLSLSIHSSSTILPSHPTPHRTLPLSECDQITLEGHVFTFYVYKPPNNNNNNTSSPIS 60
           MAS S+++HSSSTI+PSHPTPHRTL LSE DQ  LEG++F  YVYK   + +   +S ++
Sbjct: 3   MASPSVTLHSSSTIIPSHPTPHRTLTLSESDQAMLEGYIFIIYVYKKAFDID---ASSLT 62

Query: 61  IESLRTSLSQILVPYYPLAGRLRWIPRGRLELHCCAAGACFIEAEAA--AKLDDHDDFKS 120
           I+S+R SLSQILVPYYPLAGRLRWI  GRLE+ CC AG    EAEA+  AK DD+ DF+ 
Sbjct: 63  IDSMRNSLSQILVPYYPLAGRLRWIDGGRLEIDCCGAGVQLSEAEASADAKFDDYGDFEP 122

Query: 121 CDATVTNQLIPSVDYEVPIHEQPVFLVQVTRFACGGIVIGTALSHFVTDGIGAGTFINSW 180
            DA  T QL+P  DY  PI +QP+ LVQVTRF+CGG+VIG A+SHFV DGI   TFINSW
Sbjct: 123 SDA--TRQLVPKPDYRAPIEDQPLVLVQVTRFSCGGVVIGIAVSHFVVDGISVITFINSW 182

Query: 181 ARIARGESTADSVVPK-PYHERDVLRVRPRM-PSRFHHQEYEKPPLLVGHSNPEEERNRE 240
           A IARGE T +S++PK P HERD L  RP + P  FHHQEYE PPLL+GHS  EEERN++
Sbjct: 183 ASIARGEKTVESIIPKWPNHERDFL--RPVLDPLHFHHQEYESPPLLIGHSCVEEERNKQ 242

Query: 241 TIVASLKLTTCQVEKLKNRANHYHSEPNSNPKPKPYTRFEVVAGHIWVCACKARK---TC 300
           T VA LKLT  Q EKLK  AN        +   +PYTRFE V GHIW CACKAR    TC
Sbjct: 243 TTVALLKLTKHQHEKLKKIAN------EGSSMLRPYTRFEAVGGHIWACACKARNSTTTC 302

Query: 301 VTEQPTAALVTAEIRRRIRPPLPEGFVGNATLLTMTPVCKFGDLMKNPLKYAAEKIREGI 360
            ++QPTAAL+TA IR+RIRPP PEGF GNATLLT+TP+CKFGDL+KNPL YAA KIREG 
Sbjct: 303 ESDQPTAALITASIRQRIRPPPPEGFCGNATLLTVTPICKFGDLIKNPLSYAAGKIREGT 362

Query: 361 WKLTDEYVESAIDFLASKDNGWIKELYEKGTCTEGAPFWGNPNIEFGCWTSLSLYDTDFG 420
           WKLTDEY++SAI FLAS++  WIKE Y     T+  PFWGNPN++ GCWT + LYD DFG
Sbjct: 363 WKLTDEYIKSAISFLASREIEWIKEWYR----TKATPFWGNPNLDLGCWTLMPLYDADFG 422

Query: 421 WGRPRYVGLASLENLDDGESLIMSGPEKDGCLVVICMQTRHMDDFKRFFYQDL 467
           WGRP YVG   L  +DDG S+IM+GP+     VVIC+QT+HMD+FK+ FYQ L
Sbjct: 423 WGRPSYVGPIPLV-VDDGWSIIMTGPDDGSLAVVICLQTKHMDEFKKLFYQYL 457

BLAST of Spg012973 vs. NCBI nr
Match: XP_022156211.1 (spermidine hydroxycinnamoyl transferase-like [Momordica charantia])

HSP 1 Score: 556.6 bits (1433), Expect = 2.0e-154
Identity = 284/473 (60.04%), Postives = 349/473 (73.78%), Query Frame = 0

Query: 1   MASLSLSIHSSSTILPSHPTPHRTLPLSECDQITLEGHVFTFYVYKPPNNNNNNTSSPIS 60
           MA  S++IHSSSTI+PSHPTPHRTL LSE DQ  L+G+ FT YVYK   ++ +  +S ++
Sbjct: 1   MAFPSVTIHSSSTIIPSHPTPHRTLALSESDQAMLDGYSFTIYVYKKAFDHLD--ASSLA 60

Query: 61  IESLRTSLSQILVPYYPLAGRLRWIPRGRLELHCCAAGACFIEAE--AAAKLDDHDDFKS 120
           ++S+R SLS+ILVPYYPLAGRLRWI  GRLEL CC AG  FIEAE  A AKLDD+ DF  
Sbjct: 61  MDSMRNSLSKILVPYYPLAGRLRWIDGGRLELDCCGAGVRFIEAEAFADAKLDDYGDFAP 120

Query: 121 CDATVTNQLIPSVDYEVPIHEQPVFLVQVTRFACGGIVIGTALSHFVTDGIGAGTFINSW 180
            DA  T QL+P  D+  PI +QP+ LVQVTRF+CGGIVIGTA+SHFV DGI A  F+N W
Sbjct: 121 SDA--TRQLVPKPDHRAPIEDQPLVLVQVTRFSCGGIVIGTAISHFVVDGISAIMFVNLW 180

Query: 181 ARIARGESTADSVVPK-PYHERDVLRVRPRM-PSRFHHQEYEKPPLLVGHSNPEEERNRE 240
           A I +GE T +S++PK P HERD L  +P + P RFHH+EYE PPLL+G S+ +EER++E
Sbjct: 181 ASITQGEKTIESIIPKWPNHERDFL--QPVLDPPRFHHREYESPPLLIGRSDVKEERSKE 240

Query: 241 TIVASLKLTTCQVEKLKNRANHYHSEPNSNPKPKPYTRFEVVAGHIWVCACKARKT---C 300
           T VA LKL   Q EKLK  AN   + P      +PYTRFE V GHIW C CKAR     C
Sbjct: 241 TTVALLKLAKHQHEKLKKMANEGSATPQ-----RPYTRFEAVGGHIWACVCKARSNTTRC 300

Query: 301 VTEQPTAALVTAEIRRRIRPPLPEGFVGNATLLTMTPVCKFGDLMKNPLKYAAEKIREGI 360
            ++QPT AL+TA IR+RIRPP PE F GNA LLT+TP+CKFGDL+KNPL YAA  IREG 
Sbjct: 301 ESDQPTVALITASIRQRIRPPPPERFCGNAILLTVTPICKFGDLIKNPLSYAAGNIREGT 360

Query: 361 WKLTDEYVESAIDFLASKDNGWIKELYEKGTCTEGAPFWGNPNIEFGCWTSLSLYDTDFG 420
           WKLTDEY++SAI FLASK+N  +KE Y+    TE  PFWGNPN++ GCWTS+ +YD DFG
Sbjct: 361 WKLTDEYIKSAISFLASKENELVKECYQ----TEVTPFWGNPNLDLGCWTSMPIYDADFG 420

Query: 421 WGRPRYVGLASLENLDDGESLIMSGPEKDGCLVVICMQTRHMDDFKRFFYQDL 467
           WGRP YVG   L  ++DG S+IM GP+    +++IC+QT+ M++FK+ FYQ L
Sbjct: 421 WGRPSYVGPTPLV-VEDGLSIIMPGPDDGSLILIICLQTKCMNEFKKLFYQHL 457

BLAST of Spg012973 vs. NCBI nr
Match: XP_022949717.1 (spermidine hydroxycinnamoyl transferase-like [Cucurbita moschata])

HSP 1 Score: 504.6 bits (1298), Expect = 9.1e-139
Identity = 258/465 (55.48%), Postives = 320/465 (68.82%), Query Frame = 0

Query: 5   SLSIHSSSTILPSHPTPHRTLPLSECDQITLEGHVFTFYVYKPPNNNNNNTSSPISIESL 64
           SL+IHSS+T+ P+HPTP  TL  SE DQI    H  T YVY+ P       ++   +ESL
Sbjct: 3   SLTIHSSTTVFPAHPTPIATLSFSESDQIKPWTHSPTVYVYRSP-----TVTTSAVVESL 62

Query: 65  RTSLSQILVPYYPLAGRLRWIPRGRLELHCCAAGACFIEAEAAAKLDDHDDFKSCDATVT 124
           R SLSQ+LVPYYPLAGRL WI  GRLELHCC+AGA  IEA + A LDD+ DF   DA   
Sbjct: 63  RNSLSQMLVPYYPLAGRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDA--L 122

Query: 125 NQLIPSVDYEVPIHEQPVFLVQVTRFACGGIVIGTALSHFVTDGIGAGTFINSWARIARG 184
            +L P VDY  PI E PVFL+QVTRF+CGGIVIGT LSH + DG+ A TFINSWA IAR 
Sbjct: 123 RELAPKVDYSTPIEELPVFLIQVTRFSCGGIVIGTGLSHILVDGVSAITFINSWASIARK 182

Query: 185 ESTADSVVPKPYHERDVLR-VRPRMPSRFHHQEYEKPPLLVGHSNPEEERNRETIVASLK 244
           + T +S+V +P+H+R VL+  +P  P RF H+EY+ PP L+G S+ +EER +E+I   LK
Sbjct: 183 DKTVESIV-QPFHDRSVLQPQKPLRPPRFDHREYDNPPFLLGCSDAKEERKKESIPTLLK 242

Query: 245 LTTCQVEKLKNRANHYHSEPNSNPKPKPYTRFEVVAGHIWVCACKARKTCVTEQPTAALV 304
           L+  QVEKLK RAN            +PYTR+E +AGH+WVCACKAR T    QPT   V
Sbjct: 243 LSKEQVEKLKKRANSNIVNQLDELPSRPYTRYESIAGHMWVCACKARSTGDKSQPTMVRV 302

Query: 305 TAEIRRRIRPPLPEGFVGNATLLTMTPVCKFGDLMKNPLKYAAEKIREGIWKLTDEYVES 364
             ++RRR+  P+P  F GN+TL+ +TP C+FGDL+  PL YAA KIREG WK+TDEY  S
Sbjct: 303 AVDVRRRLGTPVPNNFTGNSTLVAVTPKCQFGDLISQPLSYAAGKIREGTWKITDEYARS 362

Query: 365 AIDFLAS-KDNGWIKELYEKGTCTEGAPFWGNPNIEFGCWTSLSLYDTDFGWGRPRYVGL 424
           A+DFLAS +D  W++  Y      E APFWGNPN+  G W SL LY+ DFGWG+P YVG 
Sbjct: 363 ALDFLASQEDISWVRTSYHTKVKVE-APFWGNPNLSLGSWMSLPLYEADFGWGKPCYVGP 422

Query: 425 ASLENLDDGESLIMSGPEKDGCLVV-ICMQTRHMDDFKRFFYQDL 467
           A+L    DG+S IM  P+ DG L++ I +Q +HMDDFKR FY+DL
Sbjct: 423 ATLN--ADGKSFIMPAPDNDGGLIIAIRLQKKHMDDFKRHFYEDL 456

BLAST of Spg012973 vs. NCBI nr
Match: XP_023543404.1 (spermidine hydroxycinnamoyl transferase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 500.0 bits (1286), Expect = 2.3e-137
Identity = 257/465 (55.27%), Postives = 318/465 (68.39%), Query Frame = 0

Query: 5   SLSIHSSSTILPSHPTPHRTLPLSECDQITLEGHVFTFYVYKPPNNNNNNTSSPISIESL 64
           SL+IHSS+T+ P+HPTP  TL  SE DQI    H  T YVY+ P       ++   +ESL
Sbjct: 3   SLTIHSSTTVFPAHPTPIATLSFSESDQIKPWTHSPTVYVYRSP-----AVATSTVVESL 62

Query: 65  RTSLSQILVPYYPLAGRLRWIPRGRLELHCCAAGACFIEAEAAAKLDDHDDFKSCDATVT 124
           R SLSQ+LVPYYPLAGRL WI  GRLELHCC+AGA  IEA + A LDD+ DF   DA   
Sbjct: 63  RNSLSQMLVPYYPLAGRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDA--L 122

Query: 125 NQLIPSVDYEVPIHEQPVFLVQVTRFACGGIVIGTALSHFVTDGIGAGTFINSWARIARG 184
            +L P VDY  PI E PVFL+QVTRF+CGGIVIGT LSH + DG+ A TFINSWA IAR 
Sbjct: 123 RELAPKVDYSTPIEELPVFLIQVTRFSCGGIVIGTGLSHILVDGVSAITFINSWASIARK 182

Query: 185 ESTADSVVPKPYHERDVLR-VRPRMPSRFHHQEYEKPPLLVGHSNPEEERNRETIVASLK 244
           E T +S+V +P+H+R VL+  +P    RF H+EY+ PP L+G  + +EER +E+I   LK
Sbjct: 183 EKTVESIV-QPFHDRSVLQPQKPLRAPRFDHREYDNPPFLLGCLDAKEERKKESIPTLLK 242

Query: 245 LTTCQVEKLKNRANHYHSEPNSNPKPKPYTRFEVVAGHIWVCACKARKTCVTEQPTAALV 304
           L+  QVEKLK RAN            +PYTR+E +AGH+WVCACKAR T    QPT   V
Sbjct: 243 LSKEQVEKLKKRANSNKVNQLYELPSRPYTRYESIAGHMWVCACKARSTGDNSQPTMVRV 302

Query: 305 TAEIRRRIRPPLPEGFVGNATLLTMTPVCKFGDLMKNPLKYAAEKIREGIWKLTDEYVES 364
             ++RRR+  P+P  F GN+TL+ +TP C+FGDL+  PL YAA KIREG WK+TDEY  S
Sbjct: 303 AVDVRRRLGTPVPNNFTGNSTLVAVTPKCQFGDLISQPLSYAAGKIREGTWKITDEYARS 362

Query: 365 AIDFLAS-KDNGWIKELYEKGTCTEGAPFWGNPNIEFGCWTSLSLYDTDFGWGRPRYVGL 424
           A+DFLAS +D  W++  Y      E APFWGNPN+  G W SL LY+ DFGWG+P YVG 
Sbjct: 363 ALDFLASQEDISWVRTSYHTKVKVE-APFWGNPNLSLGSWMSLPLYEADFGWGKPCYVGP 422

Query: 425 ASLENLDDGESLIMSGPEKDGCLVV-ICMQTRHMDDFKRFFYQDL 467
           A+L    DG+S IM  P+ DG L++ I +Q +HMDDFKR FY+DL
Sbjct: 423 ATLN--ADGKSFIMPAPDNDGGLIIAIRLQKKHMDDFKRHFYEDL 456

BLAST of Spg012973 vs. NCBI nr
Match: KAG6603487.1 (Spermidine hydroxycinnamoyl transferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 498.4 bits (1282), Expect = 6.6e-137
Identity = 256/465 (55.05%), Postives = 317/465 (68.17%), Query Frame = 0

Query: 5   SLSIHSSSTILPSHPTPHRTLPLSECDQITLEGHVFTFYVYKPPNNNNNNTSSPISIESL 64
           SL+IHSS+T+ P+HPTP  TL  SE DQI    H  T YVY+ P       ++   +ESL
Sbjct: 3   SLTIHSSTTVFPAHPTPIATLSFSESDQIKPWTHSPTVYVYRSP-----AVTTSAVVESL 62

Query: 65  RTSLSQILVPYYPLAGRLRWIPRGRLELHCCAAGACFIEAEAAAKLDDHDDFKSCDATVT 124
           R SLSQ+LVPYYPLAGRL WI  GRLELHCC+AGA  IEA + A LDD+ DF   DA   
Sbjct: 63  RNSLSQMLVPYYPLAGRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDA--L 122

Query: 125 NQLIPSVDYEVPIHEQPVFLVQVTRFACGGIVIGTALSHFVTDGIGAGTFINSWARIARG 184
            +L P VDY  PI E PV LVQ+TRF+CGGIVIG  +SH + DG+ A TFINSWA IAR 
Sbjct: 123 RELAPKVDYSTPIEELPVLLVQMTRFSCGGIVIGIGISHILVDGVSAITFINSWASIARK 182

Query: 185 ESTADSVVPKPYHERDVLR-VRPRMPSRFHHQEYEKPPLLVGHSNPEEERNRETIVASLK 244
           E T +S+V  P+H+R VL+  +P  P RF H+EY+ PP L+G S+ +EER +E+I   LK
Sbjct: 183 EKTVESIV-LPFHDRSVLQPQKPLRPPRFDHREYDNPPFLLGCSDAKEERKKESIPTLLK 242

Query: 245 LTTCQVEKLKNRANHYHSEPNSNPKPKPYTRFEVVAGHIWVCACKARKTCVTEQPTAALV 304
           L+  QVEKLK RAN            +PYTR+E +AGH+WVCACKAR T    QPT   V
Sbjct: 243 LSKEQVEKLKKRANSNIVTQLDELPSRPYTRYESIAGHMWVCACKARSTSDNSQPTMVRV 302

Query: 305 TAEIRRRIRPPLPEGFVGNATLLTMTPVCKFGDLMKNPLKYAAEKIREGIWKLTDEYVES 364
             ++RRR+  P+P  F GN+TL+ +TP C+FGDL+  PL YAA KIREG WK+TDEY  S
Sbjct: 303 AVDVRRRLGTPVPNNFTGNSTLVAVTPKCQFGDLISQPLSYAAGKIREGTWKITDEYARS 362

Query: 365 AIDFLAS-KDNGWIKELYEKGTCTEGAPFWGNPNIEFGCWTSLSLYDTDFGWGRPRYVGL 424
           A+DFLAS +D  W++  Y      E APFWGNPN+  G W SL LY+ DFGWG+P YVG 
Sbjct: 363 ALDFLASQEDISWVRTSYHTKVKVE-APFWGNPNLSLGSWMSLPLYEADFGWGKPCYVGP 422

Query: 425 ASLENLDDGESLIMSGPEKDGCLVV-ICMQTRHMDDFKRFFYQDL 467
           A+L    DG+S IM  P+ DG L++ I +Q +HMDDFKR FY+DL
Sbjct: 423 ATLN--ADGKSFIMPAPDNDGGLIIAIRLQKKHMDDFKRHFYEDL 456

BLAST of Spg012973 vs. ExPASy Swiss-Prot
Match: O64470 (Spermidine hydroxycinnamoyl transferase OS=Arabidopsis thaliana OX=3702 GN=SHT PE=1 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 3.3e-91
Identity = 192/469 (40.94%), Postives = 281/469 (59.91%), Query Frame = 0

Query: 6   LSIHSSSTILPSHPTPHRTLPLSECDQITLEGHVFTFYVYKPPNNNNNNTSSPISIESLR 65
           ++   S TI+P+ PT     PL+E DQ+    H+ T Y Y  P+ +         +E L+
Sbjct: 4   ITFRKSYTIVPAEPTWSGRFPLAEWDQVGTITHIPTLYFYDKPSESFQGN----VVEILK 63

Query: 66  TSLSQILVPYYPLAGRLRWIPRGRLELHCCAAGACFIEAEAAAKLDDHDDFKSCDATVTN 125
           TSLS++LV +YP+AGRLRW+PRGR EL+C A G  FIEAE+  KL D  DF         
Sbjct: 64  TSLSRVLVHFYPMAGRLRWLPRGRFELNCNAEGVEFIEAESEGKLSDFKDFS--PTPEFE 123

Query: 126 QLIPSVDYEVPIHEQPVFLVQVTRFACGGIVIGTALSHFVTDGIGAGTFINSWARIARGE 185
            L+P V+Y+ PI   P+FL QVT+F CGGI +   +SH + DG  A   I+ W R+ARGE
Sbjct: 124 NLMPQVNYKNPIETIPLFLAQVTKFKCGGISLSVNVSHAIVDGQSALHLISEWGRLARGE 183

Query: 186 STADSVVPKPYHERDVLRVRPRM-----PSRFHHQEYEKPPLLVGHSNPEEERNRETIVA 245
                +   P+ +R +L     +     P +F H+E+++PP L+G ++  EER ++TIV 
Sbjct: 184 ----PLETVPFLDRKILWAGEPLPPFVSPPKFDHKEFDQPPFLIGETDNVEERKKKTIVV 243

Query: 246 SLKLTTCQVEKLKNRAN-HYHSEPNSNPKPKPYTRFEVVAGHIWVCACKARKTCVTEQPT 305
            L L+T Q++KL+++AN   HS+P      K +TR+E V GH+W CACKAR     EQPT
Sbjct: 244 MLPLSTSQLQKLRSKANGSKHSDP-----AKGFTRYETVTGHVWRCACKARGHS-PEQPT 303

Query: 306 AALVTAEIRRRIRPPLPEGFVGNATLLTMTPVCKFGDLMKNPLKYAAEKIREGIWKLTDE 365
           A  +  + R R+ PPLP G+ GNAT L +      G+L+ N L +AA  I + I  +T+E
Sbjct: 304 ALGICIDTRSRMEPPLPRGYFGNAT-LDVVAASTSGELISNELGFAASLISKAIKNVTNE 363

Query: 366 YVESAIDFLAS-KDNGWIKELYEKGTCTEGAPFWGNPNIEFGCWTSLSLYDTDFGWGRPR 425
           YV   I++L + KD    ++L+  G+ TEG PF+GNPN+    W +L +Y  DFGWG+  
Sbjct: 364 YVMIGIEYLKNQKDLKKFQDLHALGS-TEG-PFYGNPNLGVVSWLTLPMYGLDFGWGKEF 423

Query: 426 YVGLASLENLDDGESLIMSGPEKDGCLVV-ICMQTRHMDDFKRFFYQDL 467
           Y G  + +   DG+SLI+    +DG +++  C+Q  HM+ FK+ FY+D+
Sbjct: 424 YTGPGTHD--FDGDSLILPDQNEDGSVILATCLQVAHMEAFKKHFYEDI 451

BLAST of Spg012973 vs. ExPASy Swiss-Prot
Match: Q8GSM7 (Shikimate O-hydroxycinnamoyltransferase OS=Nicotiana tabacum OX=4097 GN=HST PE=1 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 1.7e-66
Identity = 156/465 (33.55%), Postives = 240/465 (51.61%), Query Frame = 0

Query: 4   LSLSIHSSSTILPSHPTPHRTLPLSECDQITLEGHVFTFYVYKPPNNNNNNTSSPISIES 63
           + + +  S+ + P+  TP + L  S  D +    H  + Y Y+P       T SP   + 
Sbjct: 1   MKIEVKESTMVKPAAETPQQRLWNSNVDLVVPNFHTPSVYFYRP-------TGSPNFFDG 60

Query: 64  --LRTSLSQILVPYYPLAGRLRWIPRGRLELHCCAAGACFIEAEAAAKLDDHDDFKSCDA 123
             L+ +LS+ LVP+YP+AGRL     GR+E+ C   G  F+EAE+   +DD  DF     
Sbjct: 61  KVLKEALSKALVPFYPMAGRLCRDEDGRIEIDCKGQGVLFVEAESDGVVDDFGDF--APT 120

Query: 124 TVTNQLIPSVDYEVPIHEQPVFLVQVTRFACGGIVIGTALSHFVTDGIGAGTFINSWARI 183
               QLIP+VDY   I    + ++Q+T F CGG+ +G  + H   DG     FIN+W+ +
Sbjct: 121 LELRQLIPAVDYSQGIQSYALLVLQITHFKCGGVSLGVGMQHHAADGASGLHFINTWSDM 180

Query: 184 ARGESTADSVVPKPYHERDVLRVRPRMPSRFHHQEYEKPPLL--VGHSNPEEERNRETIV 243
           ARG    D  +P P+ +R +LR R     +F H EY+ PP L     + P  E   ET V
Sbjct: 181 ARG---LDLTIP-PFIDRTLLRARDPPQPQFPHVEYQPPPTLKVTPENTPISEAVPETSV 240

Query: 244 ASLKLTTCQVEKLKNRANHYHSEPNSNPKPKPYTRFEVVAGHIWVCACKARKTCVTEQPT 303
           +  KLT  Q+  LK ++    +  N       Y+ +E++AGH+W   C AR     +Q T
Sbjct: 241 SIFKLTRDQINTLKAKSKEDGNTVN-------YSSYEMLAGHVWRSTCMAR-GLAHDQET 300

Query: 304 AALVTAEIRRRIRPPLPEGFVGNATLLTMTPVCKFGDLMKNPLKYAAEKIREGIWKLTDE 363
              +  + R R+RP LP G+ GN  + T TP+   GD+   P+ YAA K+ + + ++ ++
Sbjct: 301 KLYIATDGRSRLRPSLPPGYFGN-VIFTTTPIAVAGDIQSKPIWYAASKLHDALARMDND 360

Query: 364 YVESAIDFLASKDNGWIKELYEKGTCTEGAPFWGNPNIEFGCWTSLSLYDTDFGWGRPRY 423
           Y+ SA+D+L  + +  +K L        GA  +  PN+    W+ L ++D DFGWGRP +
Sbjct: 361 YLRSALDYLELQPD--LKAL------VRGAHTFKCPNLGITSWSRLPIHDADFGWGRPIF 420

Query: 424 VGLASLENLDDGESLIMSGPEKDGC-LVVICMQTRHMDDFKRFFY 464
           +G   +    +G S I+  P  DG   V I +Q  HM  F++F Y
Sbjct: 421 MGPGGIAY--EGLSFILPSPTNDGSQSVAISLQAEHMKLFEKFLY 433

BLAST of Spg012973 vs. ExPASy Swiss-Prot
Match: Q9FI78 (Shikimate O-hydroxycinnamoyltransferase OS=Arabidopsis thaliana OX=3702 GN=HST PE=1 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 1.8e-65
Identity = 151/462 (32.68%), Postives = 235/462 (50.87%), Query Frame = 0

Query: 4   LSLSIHSSSTILPSHPTPHRTLPLSECDQITLEGHVFTFYVYKPPNNNNNNTSSPISIES 63
           + ++I  S+ + P+  TP   L  S  D +    H  + Y Y+P        S+    + 
Sbjct: 1   MKINIRDSTMVRPATETPITNLWNSNVDLVIPRFHTPSVYFYRP-----TGASNFFDPQV 60

Query: 64  LRTSLSQILVPYYPLAGRLRWIPRGRLELHCCAAGACFIEAEAAAKLDDHDDFKSCDATV 123
           ++ +LS+ LVP+YP+AGRL+    GR+E+ C  AG  F+ A+  + +DD  DF       
Sbjct: 61  MKEALSKALVPFYPMAGRLKRDDDGRIEIDCNGAGVLFVVADTPSVIDDFGDF--APTLN 120

Query: 124 TNQLIPSVDYEVPIHEQPVFLVQVTRFACGGIVIGTALSHFVTDGIGAGTFINSWARIAR 183
             QLIP VD+   IH  P+ ++QVT F CGG  +G  + H   DG     FIN+W+ +AR
Sbjct: 121 LRQLIPEVDHSAGIHSFPLLVLQVTFFKCGGASLGVGMQHHAADGFSGLHFINTWSDMAR 180

Query: 184 GESTADSVVPKPYHERDVLRVRPRMPSRFHHQEYEKPPLLVGHSNPEEERNRETIVASLK 243
           G    D  +P P+ +R +LR R      FHH EY+  P +    +P +     T V+  K
Sbjct: 181 G---LDLTIP-PFIDRTLLRARDPPQPAFHHVEYQPAPSMKIPLDPSKSGPENTTVSIFK 240

Query: 244 LTTCQVEKLKNRANHYHSEPNSNPKPKPYTRFEVVAGHIWVCACKARKTCVTEQPTAALV 303
           LT  Q+  LK       ++   +     Y+ +E++AGH+W    KAR     +Q T   +
Sbjct: 241 LTRDQLVALK-------AKSKEDGNTVSYSSYEMLAGHVWRSVGKAR-GLPNDQETKLYI 300

Query: 304 TAEIRRRIRPPLPEGFVGNATLLTMTPVCKFGDLMKNPLKYAAEKIREGIWKLTDEYVES 363
             + R R+RP LP G+ GN  + T TP+   GDL+  P  YAA +I + + ++ D Y+ S
Sbjct: 301 ATDGRSRLRPQLPPGYFGN-VIFTATPLAVAGDLLSKPTWYAAGQIHDFLVRMDDNYLRS 360

Query: 364 AIDFLASKDNGWIKELYEKGTCTEGAPFWGNPNIEFGCWTSLSLYDTDFGWGRPRYVGLA 423
           A+D+L  +         +      GA  +  PN+    W  L +YD DFGWGRP ++G  
Sbjct: 361 ALDYLEMQP--------DLSALVRGAHTYKCPNLGITSWVRLPIYDADFGWGRPIFMGPG 420

Query: 424 SLENLDDGESLIMSGPEKDGCL-VVICMQTRHMDDFKRFFYQ 465
            +    +G S ++  P  DG L V I +Q+ HM  F++F ++
Sbjct: 421 GIPY--EGLSFVLPSPTNDGSLSVAIALQSEHMKLFEKFLFE 432

BLAST of Spg012973 vs. ExPASy Swiss-Prot
Match: A0PDV5 (Rosmarinate synthase OS=Plectranthus scutellarioides OX=4142 GN=RAS PE=1 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 8.8e-60
Identity = 157/463 (33.91%), Postives = 241/463 (52.05%), Query Frame = 0

Query: 4   LSLSIHSSSTILPSHPTPHRTLPLSECDQIT-LEGHVFTFYVYKPPNNNNNNTSSPISIE 63
           + + +  S+ I PS  TP  +L LS  D ++    H  + + Y    ++N          
Sbjct: 1   MKIEVKDSTMIKPSAETPGGSLWLSNLDLLSPANYHTLSVHFYSHDGSDNF-----FDAA 60

Query: 64  SLRTSLSQILVPYYPLAGRLRWIPRGRLELHCCAAGACFIEAEAAAKLDDHDDFKSCDAT 123
            L+ SLS+ LV +YP AGRL+ +   RLE+ C   G   +EAE    LD+  DF      
Sbjct: 61  GLKESLSRALVEFYPYAGRLK-LNGNRLEIDCNNEGLLLVEAECDGALDELGDFAPRPEL 120

Query: 124 VTNQLIPSVDYEVPIHEQPVFLVQVTRFACGGIVIGTALSHFVTDGIGAGTFINSWARIA 183
               LIP VDY   I   P+ + Q+TRF CGG+ +G A  H ++DG+ A  FIN+WA ++
Sbjct: 121 ---NLIPKVDYSRGISTYPLMVFQLTRFKCGGVALGVANEHHLSDGVAALHFINTWAHLS 180

Query: 184 RGESTADSVVPKPYHERDVLRVRPRMPSRFHHQEYEKPPLLVGHSNPEEERNRETIVASL 243
           RG   A +  P P+ +R  L  R     +F H EY+ PP L    NP    + +   +  
Sbjct: 181 RG---APAPTPLPHFDRSSLSARNPPQPQFSHAEYQPPPTL---ENPLP--HTDIAHSRF 240

Query: 244 KLTTCQVEKLKNRANHYHSEPNSNPKPKPYTRFEVVAGHIWVCACKARKTCVTEQPTAAL 303
           KLT  Q+  LK++   + + P      K Y+ FEV+AGHIW   C AR      Q T   
Sbjct: 241 KLTRDQLNSLKSK---FKTAPADGGAGKSYSTFEVLAGHIWRSVCIAR-GLPEGQETKLH 300

Query: 304 VTAEIRRRIRPPLPEGFVGNATLLTMTPVCKFGDLMKNPLKYAAEKIREGIWKLTDEYVE 363
           +  + R R++  LP GF GNA +   TP+   G++  N L YA  ++ +G+ +L ++Y+ 
Sbjct: 301 IPFDGRGRLQ--LPPGFFGNA-IFFATPIATCGEIESNSLNYAVRRVSDGVSRLDEDYLR 360

Query: 364 SAIDFLASKDNGWIKELYEKGTCTEGAPFWGNPNIEFGCWTSLSLYDTDFGWGRPRYVGL 423
           S+IDFL  +++  I +L       +GA  +  PN+    W  L +Y+ DFGWG+  Y+G 
Sbjct: 361 SSIDFLELQED--ISKL------AQGAHSFRCPNLWVISWVWLPIYEPDFGWGKAVYMGP 420

Query: 424 ASLENLDDGESLIMSGPEKDGCLVV-ICMQTRHMDDFKRFFYQ 465
            +     +G+S ++  PEKDG L V I +  +HM+ F++ FY+
Sbjct: 421 WAAP--FEGKSYLLPNPEKDGSLFVSITLHKQHMERFEKLFYE 429

BLAST of Spg012973 vs. ExPASy Swiss-Prot
Match: A0A2H5AIZ1 (Hydroxycinnamoyltransferase OS=Narcissus pseudonarcissus OX=39639 GN=HCT PE=2 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 2.6e-59
Identity = 151/467 (32.33%), Postives = 231/467 (49.46%), Query Frame = 0

Query: 6   LSIHSSSTILPSHPTPHRTLPLSECDQITLEGHVFTFYVYKPPNNNNNNTSSPISIESLR 65
           ++I  ++ + PS PTP + L  S  D +    H  + Y Y+ P     N S       L+
Sbjct: 3   INIKETTMVRPSQPTPSQRLWNSNLDLVVPRFHTPSVYFYRRP----GNASDFFDARVLK 62

Query: 66  TSLSQILVPYYPLAGRLRWIPRGRLELHCCAAGACFIEAEAAAKLDDHDDFKSCDATVTN 125
            +L + LVP+YP+AGRL     GR+E+ C   G  F+ AE  + +D+  DF         
Sbjct: 63  EALGRALVPFYPMAGRLARDEDGRVEIDCNGEGVRFVVAETDSAIDEFGDF--APTMELK 122

Query: 126 QLIPSVDYEVPIHEQPVFLVQVTRFACGGIVIGTALSHFVTDGIGAGTFINSWARIARGE 185
           +LIP V+Y   I   P+ ++Q+T F CGG  +G  + H V DG     FINSW+ IARG 
Sbjct: 123 KLIPKVEYGDDISAFPLLVLQITHFKCGGTSLGVGMQHHVADGASGLHFINSWSDIARG- 182

Query: 186 STADSVVPKPYHERDVLRVRPRMPSRFHHQEYEKPPLLVGHSNPEEERN-----RETIVA 245
              D  VP P+ +R +LR R      F H EY+  P +     P ++         T V+
Sbjct: 183 --LDIAVP-PFIDRSLLRARDPPSPSFPHIEYQPAPSMNTSPAPIQDPTVKSDPTATAVS 242

Query: 246 SLKLTTCQVEKLKNRANHYHSEPNSNPKPKPYTRFEVVAGHIWVCACKARKTCVTEQPTA 305
             KLT  Q++ LK+R +              Y+ + +VAGH+W C   AR     +Q T 
Sbjct: 243 IFKLTKQQLDLLKSRVS------------AKYSSYALVAGHVWRCTSIAR-GLPDDQRTK 302

Query: 306 ALVTAEIRRRIRPPLPEGFVGNATLLTMTPVCKFGDLM--KNPLKYAAEKIREGIWKLTD 365
                + R R++PPLP G+ GN  + T TPV   G++    + L+ AA +I+  + ++ D
Sbjct: 303 LYCATDGRARLQPPLPSGYFGN-VIFTATPVADAGEITGEDSGLEAAAGRIQRALMRMDD 362

Query: 366 EYVESAIDFLASKDNGWIKELYEKGTCTEGAPFWGNPNIEFGCWTSLSLYDTDFGWGRPR 425
           EY+ SA+D+L  +         +      GA  +  PNI    WT L ++D DFGWGRP 
Sbjct: 363 EYLRSALDYLELQP--------DLSKLVRGAHTFRCPNIGLTSWTRLPIHDADFGWGRPI 422

Query: 426 YVGLASLENLDDGESLIMSGPEKDGCL-VVICMQTRHMDDFKRFFYQ 465
           ++G   +    +G + ++   E DG L + I +Q  HM  F++  Y+
Sbjct: 423 FMGPGGIAY--EGLAFMLPSSEGDGSLSIAISLQAEHMIKFQKLLYE 435

BLAST of Spg012973 vs. ExPASy TrEMBL
Match: A0A6J1DQ54 (spermidine hydroxycinnamoyl transferase-like OS=Momordica charantia OX=3673 GN=LOC111023192 PE=3 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 1.7e-162
Identity = 298/473 (63.00%), Postives = 353/473 (74.63%), Query Frame = 0

Query: 1   MASLSLSIHSSSTILPSHPTPHRTLPLSECDQITLEGHVFTFYVYKPPNNNNNNTSSPIS 60
           MAS S+++HSSSTI+PSHPTPHRTL LSE DQ  LEG++F  YVYK   + +   +S ++
Sbjct: 3   MASPSVTLHSSSTIIPSHPTPHRTLTLSESDQAMLEGYIFIIYVYKKAFDID---ASSLT 62

Query: 61  IESLRTSLSQILVPYYPLAGRLRWIPRGRLELHCCAAGACFIEAEAA--AKLDDHDDFKS 120
           I+S+R SLSQILVPYYPLAGRLRWI  GRLE+ CC AG    EAEA+  AK DD+ DF+ 
Sbjct: 63  IDSMRNSLSQILVPYYPLAGRLRWIDGGRLEIDCCGAGVQLSEAEASADAKFDDYGDFEP 122

Query: 121 CDATVTNQLIPSVDYEVPIHEQPVFLVQVTRFACGGIVIGTALSHFVTDGIGAGTFINSW 180
            DA  T QL+P  DY  PI +QP+ LVQVTRF+CGG+VIG A+SHFV DGI   TFINSW
Sbjct: 123 SDA--TRQLVPKPDYRAPIEDQPLVLVQVTRFSCGGVVIGIAVSHFVVDGISVITFINSW 182

Query: 181 ARIARGESTADSVVPK-PYHERDVLRVRPRM-PSRFHHQEYEKPPLLVGHSNPEEERNRE 240
           A IARGE T +S++PK P HERD L  RP + P  FHHQEYE PPLL+GHS  EEERN++
Sbjct: 183 ASIARGEKTVESIIPKWPNHERDFL--RPVLDPLHFHHQEYESPPLLIGHSCVEEERNKQ 242

Query: 241 TIVASLKLTTCQVEKLKNRANHYHSEPNSNPKPKPYTRFEVVAGHIWVCACKARK---TC 300
           T VA LKLT  Q EKLK  AN        +   +PYTRFE V GHIW CACKAR    TC
Sbjct: 243 TTVALLKLTKHQHEKLKKIAN------EGSSMLRPYTRFEAVGGHIWACACKARNSTTTC 302

Query: 301 VTEQPTAALVTAEIRRRIRPPLPEGFVGNATLLTMTPVCKFGDLMKNPLKYAAEKIREGI 360
            ++QPTAAL+TA IR+RIRPP PEGF GNATLLT+TP+CKFGDL+KNPL YAA KIREG 
Sbjct: 303 ESDQPTAALITASIRQRIRPPPPEGFCGNATLLTVTPICKFGDLIKNPLSYAAGKIREGT 362

Query: 361 WKLTDEYVESAIDFLASKDNGWIKELYEKGTCTEGAPFWGNPNIEFGCWTSLSLYDTDFG 420
           WKLTDEY++SAI FLAS++  WIKE Y     T+  PFWGNPN++ GCWT + LYD DFG
Sbjct: 363 WKLTDEYIKSAISFLASREIEWIKEWYR----TKATPFWGNPNLDLGCWTLMPLYDADFG 422

Query: 421 WGRPRYVGLASLENLDDGESLIMSGPEKDGCLVVICMQTRHMDDFKRFFYQDL 467
           WGRP YVG   L  +DDG S+IM+GP+     VVIC+QT+HMD+FK+ FYQ L
Sbjct: 423 WGRPSYVGPIPLV-VDDGWSIIMTGPDDGSLAVVICLQTKHMDEFKKLFYQYL 457

BLAST of Spg012973 vs. ExPASy TrEMBL
Match: A0A6J1DU82 (spermidine hydroxycinnamoyl transferase-like OS=Momordica charantia OX=3673 GN=LOC111023154 PE=3 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 9.8e-155
Identity = 284/473 (60.04%), Postives = 349/473 (73.78%), Query Frame = 0

Query: 1   MASLSLSIHSSSTILPSHPTPHRTLPLSECDQITLEGHVFTFYVYKPPNNNNNNTSSPIS 60
           MA  S++IHSSSTI+PSHPTPHRTL LSE DQ  L+G+ FT YVYK   ++ +  +S ++
Sbjct: 1   MAFPSVTIHSSSTIIPSHPTPHRTLALSESDQAMLDGYSFTIYVYKKAFDHLD--ASSLA 60

Query: 61  IESLRTSLSQILVPYYPLAGRLRWIPRGRLELHCCAAGACFIEAE--AAAKLDDHDDFKS 120
           ++S+R SLS+ILVPYYPLAGRLRWI  GRLEL CC AG  FIEAE  A AKLDD+ DF  
Sbjct: 61  MDSMRNSLSKILVPYYPLAGRLRWIDGGRLELDCCGAGVRFIEAEAFADAKLDDYGDFAP 120

Query: 121 CDATVTNQLIPSVDYEVPIHEQPVFLVQVTRFACGGIVIGTALSHFVTDGIGAGTFINSW 180
            DA  T QL+P  D+  PI +QP+ LVQVTRF+CGGIVIGTA+SHFV DGI A  F+N W
Sbjct: 121 SDA--TRQLVPKPDHRAPIEDQPLVLVQVTRFSCGGIVIGTAISHFVVDGISAIMFVNLW 180

Query: 181 ARIARGESTADSVVPK-PYHERDVLRVRPRM-PSRFHHQEYEKPPLLVGHSNPEEERNRE 240
           A I +GE T +S++PK P HERD L  +P + P RFHH+EYE PPLL+G S+ +EER++E
Sbjct: 181 ASITQGEKTIESIIPKWPNHERDFL--QPVLDPPRFHHREYESPPLLIGRSDVKEERSKE 240

Query: 241 TIVASLKLTTCQVEKLKNRANHYHSEPNSNPKPKPYTRFEVVAGHIWVCACKARKT---C 300
           T VA LKL   Q EKLK  AN   + P      +PYTRFE V GHIW C CKAR     C
Sbjct: 241 TTVALLKLAKHQHEKLKKMANEGSATPQ-----RPYTRFEAVGGHIWACVCKARSNTTRC 300

Query: 301 VTEQPTAALVTAEIRRRIRPPLPEGFVGNATLLTMTPVCKFGDLMKNPLKYAAEKIREGI 360
            ++QPT AL+TA IR+RIRPP PE F GNA LLT+TP+CKFGDL+KNPL YAA  IREG 
Sbjct: 301 ESDQPTVALITASIRQRIRPPPPERFCGNAILLTVTPICKFGDLIKNPLSYAAGNIREGT 360

Query: 361 WKLTDEYVESAIDFLASKDNGWIKELYEKGTCTEGAPFWGNPNIEFGCWTSLSLYDTDFG 420
           WKLTDEY++SAI FLASK+N  +KE Y+    TE  PFWGNPN++ GCWTS+ +YD DFG
Sbjct: 361 WKLTDEYIKSAISFLASKENELVKECYQ----TEVTPFWGNPNLDLGCWTSMPIYDADFG 420

Query: 421 WGRPRYVGLASLENLDDGESLIMSGPEKDGCLVVICMQTRHMDDFKRFFYQDL 467
           WGRP YVG   L  ++DG S+IM GP+    +++IC+QT+ M++FK+ FYQ L
Sbjct: 421 WGRPSYVGPTPLV-VEDGLSIIMPGPDDGSLILIICLQTKCMNEFKKLFYQHL 457

BLAST of Spg012973 vs. ExPASy TrEMBL
Match: A0A6J1GCX6 (spermidine hydroxycinnamoyl transferase-like OS=Cucurbita moschata OX=3662 GN=LOC111453028 PE=3 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 4.4e-139
Identity = 258/465 (55.48%), Postives = 320/465 (68.82%), Query Frame = 0

Query: 5   SLSIHSSSTILPSHPTPHRTLPLSECDQITLEGHVFTFYVYKPPNNNNNNTSSPISIESL 64
           SL+IHSS+T+ P+HPTP  TL  SE DQI    H  T YVY+ P       ++   +ESL
Sbjct: 3   SLTIHSSTTVFPAHPTPIATLSFSESDQIKPWTHSPTVYVYRSP-----TVTTSAVVESL 62

Query: 65  RTSLSQILVPYYPLAGRLRWIPRGRLELHCCAAGACFIEAEAAAKLDDHDDFKSCDATVT 124
           R SLSQ+LVPYYPLAGRL WI  GRLELHCC+AGA  IEA + A LDD+ DF   DA   
Sbjct: 63  RNSLSQMLVPYYPLAGRLHWIGGGRLELHCCSAGAQLIEASSTATLDDYGDFSPTDA--L 122

Query: 125 NQLIPSVDYEVPIHEQPVFLVQVTRFACGGIVIGTALSHFVTDGIGAGTFINSWARIARG 184
            +L P VDY  PI E PVFL+QVTRF+CGGIVIGT LSH + DG+ A TFINSWA IAR 
Sbjct: 123 RELAPKVDYSTPIEELPVFLIQVTRFSCGGIVIGTGLSHILVDGVSAITFINSWASIARK 182

Query: 185 ESTADSVVPKPYHERDVLR-VRPRMPSRFHHQEYEKPPLLVGHSNPEEERNRETIVASLK 244
           + T +S+V +P+H+R VL+  +P  P RF H+EY+ PP L+G S+ +EER +E+I   LK
Sbjct: 183 DKTVESIV-QPFHDRSVLQPQKPLRPPRFDHREYDNPPFLLGCSDAKEERKKESIPTLLK 242

Query: 245 LTTCQVEKLKNRANHYHSEPNSNPKPKPYTRFEVVAGHIWVCACKARKTCVTEQPTAALV 304
           L+  QVEKLK RAN            +PYTR+E +AGH+WVCACKAR T    QPT   V
Sbjct: 243 LSKEQVEKLKKRANSNIVNQLDELPSRPYTRYESIAGHMWVCACKARSTGDKSQPTMVRV 302

Query: 305 TAEIRRRIRPPLPEGFVGNATLLTMTPVCKFGDLMKNPLKYAAEKIREGIWKLTDEYVES 364
             ++RRR+  P+P  F GN+TL+ +TP C+FGDL+  PL YAA KIREG WK+TDEY  S
Sbjct: 303 AVDVRRRLGTPVPNNFTGNSTLVAVTPKCQFGDLISQPLSYAAGKIREGTWKITDEYARS 362

Query: 365 AIDFLAS-KDNGWIKELYEKGTCTEGAPFWGNPNIEFGCWTSLSLYDTDFGWGRPRYVGL 424
           A+DFLAS +D  W++  Y      E APFWGNPN+  G W SL LY+ DFGWG+P YVG 
Sbjct: 363 ALDFLASQEDISWVRTSYHTKVKVE-APFWGNPNLSLGSWMSLPLYEADFGWGKPCYVGP 422

Query: 425 ASLENLDDGESLIMSGPEKDGCLVV-ICMQTRHMDDFKRFFYQDL 467
           A+L    DG+S IM  P+ DG L++ I +Q +HMDDFKR FY+DL
Sbjct: 423 ATLN--ADGKSFIMPAPDNDGGLIIAIRLQKKHMDDFKRHFYEDL 456

BLAST of Spg012973 vs. ExPASy TrEMBL
Match: A0A6J1DRJ9 (spermidine hydroxycinnamoyl transferase-like OS=Momordica charantia OX=3673 GN=LOC111023179 PE=3 SV=1)

HSP 1 Score: 496.9 bits (1278), Expect = 9.2e-137
Identity = 256/476 (53.78%), Postives = 326/476 (68.49%), Query Frame = 0

Query: 5   SLSIHSSSTILPSHPTPHRTLPLSECDQITLEGHVFTFYVYKPPNNNNNNTSSPISIESL 64
           SL+I+SS+T+ PSHPTP   L LSE DQ     HV T Y+YK P       ++   + SL
Sbjct: 3   SLTINSSTTVFPSHPTPAAALSLSENDQFRAWTHVPTVYIYKSP----AAAATAAVLGSL 62

Query: 65  RTSLSQILVPYYPLAGRLRWIPRGRLELHCCAAGACFIEAEAAAKLDDHDDFKSCDATVT 124
           + SLSQILVPYYPLAGRLR I  GRLEL CCAAGA  +EA + AKLDD+ DF   DA   
Sbjct: 63  KNSLSQILVPYYPLAGRLRLIAGGRLELDCCAAGATLMEASSTAKLDDYGDFAPSDA--L 122

Query: 125 NQLIPSVDYE-VPIHEQPVFLVQVTRFACGGIVIGTALSHFVTDGIGAGTFINSWARIAR 184
            +L P+VDY+  PI E P+ LVQVTRF+CGG+V+G   SH + DG  A TFINSWA +AR
Sbjct: 123 RRLAPNVDYDNSPIEEVPMLLVQVTRFSCGGLVVGVTFSHTLVDGASAITFINSWASMAR 182

Query: 185 GESTADSVVPKPYHERDVLR-VRPRMPSRFHHQEYEKPPLLVGHSNPEEERNRETIVASL 244
           GE T DS +P+P+H+R  L+  +P  P RFHH EY++PPLL G+SN EEE  +E++ A L
Sbjct: 183 GEKTVDS-IPQPFHDRKFLQPQKPVPPPRFHHGEYDEPPLLHGYSNAEEEEKKESVCALL 242

Query: 245 KLTTCQVEKLKNRANHYHSEPNSNP----------KPKPYTRFEVVAGHIWVCACKARKT 304
           KLT  QVEKLK RAN     PN+            +P+PY+R+E + G++W+CACKAR  
Sbjct: 243 KLTKEQVEKLKKRANSNQPNPNTTNQNMAMLDHELQPRPYSRYESITGYMWLCACKARNN 302

Query: 305 CVTEQPTAALVTAEIRRRIRPPLPEGFVGNATLLTMTPVCKFGDLMKNPLKYAAEKIREG 364
             + QPT   +  +I RR+R P+PE F GN+ L+T+TP CKFGDLM  PL YA  KIREG
Sbjct: 303 AESRQPTVVRIIVDISRRLRTPIPENFTGNSCLITITPQCKFGDLMTQPLSYAVGKIREG 362

Query: 365 IWKLTDEYVESAIDFLASK-DNGWIKELYEKGTCTEGAPFWGNPNIEFGCWTSLSLYDTD 424
            WK+TDEY +SA+DFLA K D  W + L+      +G+ FWGNPN+  G W S+ LYD D
Sbjct: 363 TWKMTDEYAKSAMDFLARKEDITWARTLHHSIVRVKGS-FWGNPNLALGSWMSMPLYDAD 422

Query: 425 FGWGRPRYVGLASLENLDDGESLIMSGPEKDGCLVV-ICMQTRHMDDFKRFFYQDL 467
           FGWGRP YVG A + +  DG++ IMSGP  DG +++ I +QT+HMDDFK+FFY+DL
Sbjct: 423 FGWGRPSYVGPAMVNS--DGKAYIMSGPTNDGSVIIAIRLQTKHMDDFKKFFYEDL 468

BLAST of Spg012973 vs. ExPASy TrEMBL
Match: A0A6J1HTD7 (spermidine hydroxycinnamoyl transferase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466005 PE=3 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 5.1e-135
Identity = 246/470 (52.34%), Postives = 319/470 (67.87%), Query Frame = 0

Query: 5   SLSIHSSSTILPSHPTPHRTLPLSECDQITLEGHVFTFYVYKPPNNNNNNTSSPISIESL 64
           S++IHSSST++PSHPTP  T   SECDQ     H+ T YVYK P       +  +++ESL
Sbjct: 3   SITIHSSSTVVPSHPTPTGTFSFSECDQYRAWTHLPTIYVYKSP------ATEVVAVESL 62

Query: 65  RTSLSQILVPYYPLAGRLRWIPRGRLELHCCAAGACFIEAEAAAKLDDHDDFKSCDATVT 124
           +TSLSQILVPYYPLAGRL W+P GRLEL CCAAG  F+EA   AKL+D+ DF   DA   
Sbjct: 63  KTSLSQILVPYYPLAGRLHWLPGGRLELDCCAAGVLFVEASTDAKLEDYGDFAPSDA--V 122

Query: 125 NQLIPSVDYEVPIHEQPVFLVQVTRFACGGIVIGTALSHFVTDGIGAGTFINSWARIARG 184
            QLIP+VDY  PI E P+ L QVTRF+CGG+VIG A  H V DG  A TFINSWA +ARG
Sbjct: 123 RQLIPTVDYNSPIEELPLLLFQVTRFSCGGVVIGVANCHTVVDGASAITFINSWASMARG 182

Query: 185 ESTADSVVPKPYHERDVLR-VRPRMPSRFHHQEYEK-PPLLVGHSNPEEERNRETIVASL 244
           E T ++++ +P H+R  L   +P  P RF H E+ K PP+++G SNPEEE  +ET  A L
Sbjct: 183 EKTVETIL-QPLHDRKFLMPEKPLRPPRFDHIEFTKPPPVILGSSNPEEEAKQETTSALL 242

Query: 245 KLTTCQVEKLKNRANHYHSEPN----SNPKPKPYTRFEVVAGHIWVCACKARKTCVTEQP 304
           KLT  Q+EKLK +AN   +          +P+PY+RFE + GH+WVCACKAR T  + QP
Sbjct: 243 KLTKEQIEKLKKKANSNLTNQTMGVADEVQPRPYSRFEAITGHMWVCACKARNTGESRQP 302

Query: 305 TAALVTAEIRRRIRPPLPEGFVGNATLLTMTPVCKFGDLMKNPLKYAAEKIREGIWKLTD 364
           T   +  ++RRR++PP+PE F GN++ + +TP C+FG+LM  PL YAA K+REG WK+T 
Sbjct: 303 TVIRIPIDVRRRMQPPIPENFSGNSSFVVLTPECEFGELMSQPLSYAAGKVREGTWKMTG 362

Query: 365 EYVESAIDFLASKDN-GWIKELYEKGTCTEGAPFWGNPNIEFGCWTSLSLYDTDFGWGRP 424
           EY +S +D+LA +D+  W++  +      + A FWGNPN+  G W SL  Y+ DFGWGRP
Sbjct: 363 EYAQSVVDYLAGQDDISWLRTSFHSKVLVK-ASFWGNPNLSIGSWISLPFYEADFGWGRP 422

Query: 425 RYVGLASLENLDDGESLIMSGPEKDGCLVV-ICMQTRHMDDFKRFFYQDL 467
            Y G A+L    DG+S IM GP  DG L+V I +Q RHMD FK++FY+D+
Sbjct: 423 SYFGPATLN--IDGKSYIMVGPSDDGSLIVAIRLQARHMDGFKKYFYEDI 460

BLAST of Spg012973 vs. TAIR 10
Match: AT2G19070.1 (spermidine hydroxycinnamoyl transferase )

HSP 1 Score: 337.0 bits (863), Expect = 2.4e-92
Identity = 192/469 (40.94%), Postives = 281/469 (59.91%), Query Frame = 0

Query: 6   LSIHSSSTILPSHPTPHRTLPLSECDQITLEGHVFTFYVYKPPNNNNNNTSSPISIESLR 65
           ++   S TI+P+ PT     PL+E DQ+    H+ T Y Y  P+ +         +E L+
Sbjct: 4   ITFRKSYTIVPAEPTWSGRFPLAEWDQVGTITHIPTLYFYDKPSESFQGN----VVEILK 63

Query: 66  TSLSQILVPYYPLAGRLRWIPRGRLELHCCAAGACFIEAEAAAKLDDHDDFKSCDATVTN 125
           TSLS++LV +YP+AGRLRW+PRGR EL+C A G  FIEAE+  KL D  DF         
Sbjct: 64  TSLSRVLVHFYPMAGRLRWLPRGRFELNCNAEGVEFIEAESEGKLSDFKDFS--PTPEFE 123

Query: 126 QLIPSVDYEVPIHEQPVFLVQVTRFACGGIVIGTALSHFVTDGIGAGTFINSWARIARGE 185
            L+P V+Y+ PI   P+FL QVT+F CGGI +   +SH + DG  A   I+ W R+ARGE
Sbjct: 124 NLMPQVNYKNPIETIPLFLAQVTKFKCGGISLSVNVSHAIVDGQSALHLISEWGRLARGE 183

Query: 186 STADSVVPKPYHERDVLRVRPRM-----PSRFHHQEYEKPPLLVGHSNPEEERNRETIVA 245
                +   P+ +R +L     +     P +F H+E+++PP L+G ++  EER ++TIV 
Sbjct: 184 ----PLETVPFLDRKILWAGEPLPPFVSPPKFDHKEFDQPPFLIGETDNVEERKKKTIVV 243

Query: 246 SLKLTTCQVEKLKNRAN-HYHSEPNSNPKPKPYTRFEVVAGHIWVCACKARKTCVTEQPT 305
            L L+T Q++KL+++AN   HS+P      K +TR+E V GH+W CACKAR     EQPT
Sbjct: 244 MLPLSTSQLQKLRSKANGSKHSDP-----AKGFTRYETVTGHVWRCACKARGHS-PEQPT 303

Query: 306 AALVTAEIRRRIRPPLPEGFVGNATLLTMTPVCKFGDLMKNPLKYAAEKIREGIWKLTDE 365
           A  +  + R R+ PPLP G+ GNAT L +      G+L+ N L +AA  I + I  +T+E
Sbjct: 304 ALGICIDTRSRMEPPLPRGYFGNAT-LDVVAASTSGELISNELGFAASLISKAIKNVTNE 363

Query: 366 YVESAIDFLAS-KDNGWIKELYEKGTCTEGAPFWGNPNIEFGCWTSLSLYDTDFGWGRPR 425
           YV   I++L + KD    ++L+  G+ TEG PF+GNPN+    W +L +Y  DFGWG+  
Sbjct: 364 YVMIGIEYLKNQKDLKKFQDLHALGS-TEG-PFYGNPNLGVVSWLTLPMYGLDFGWGKEF 423

Query: 426 YVGLASLENLDDGESLIMSGPEKDGCLVV-ICMQTRHMDDFKRFFYQDL 467
           Y G  + +   DG+SLI+    +DG +++  C+Q  HM+ FK+ FY+D+
Sbjct: 424 YTGPGTHD--FDGDSLILPDQNEDGSVILATCLQVAHMEAFKKHFYEDI 451

BLAST of Spg012973 vs. TAIR 10
Match: AT5G48930.1 (hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase )

HSP 1 Score: 251.5 bits (641), Expect = 1.3e-66
Identity = 151/462 (32.68%), Postives = 235/462 (50.87%), Query Frame = 0

Query: 4   LSLSIHSSSTILPSHPTPHRTLPLSECDQITLEGHVFTFYVYKPPNNNNNNTSSPISIES 63
           + ++I  S+ + P+  TP   L  S  D +    H  + Y Y+P        S+    + 
Sbjct: 1   MKINIRDSTMVRPATETPITNLWNSNVDLVIPRFHTPSVYFYRP-----TGASNFFDPQV 60

Query: 64  LRTSLSQILVPYYPLAGRLRWIPRGRLELHCCAAGACFIEAEAAAKLDDHDDFKSCDATV 123
           ++ +LS+ LVP+YP+AGRL+    GR+E+ C  AG  F+ A+  + +DD  DF       
Sbjct: 61  MKEALSKALVPFYPMAGRLKRDDDGRIEIDCNGAGVLFVVADTPSVIDDFGDF--APTLN 120

Query: 124 TNQLIPSVDYEVPIHEQPVFLVQVTRFACGGIVIGTALSHFVTDGIGAGTFINSWARIAR 183
             QLIP VD+   IH  P+ ++QVT F CGG  +G  + H   DG     FIN+W+ +AR
Sbjct: 121 LRQLIPEVDHSAGIHSFPLLVLQVTFFKCGGASLGVGMQHHAADGFSGLHFINTWSDMAR 180

Query: 184 GESTADSVVPKPYHERDVLRVRPRMPSRFHHQEYEKPPLLVGHSNPEEERNRETIVASLK 243
           G    D  +P P+ +R +LR R      FHH EY+  P +    +P +     T V+  K
Sbjct: 181 G---LDLTIP-PFIDRTLLRARDPPQPAFHHVEYQPAPSMKIPLDPSKSGPENTTVSIFK 240

Query: 244 LTTCQVEKLKNRANHYHSEPNSNPKPKPYTRFEVVAGHIWVCACKARKTCVTEQPTAALV 303
           LT  Q+  LK       ++   +     Y+ +E++AGH+W    KAR     +Q T   +
Sbjct: 241 LTRDQLVALK-------AKSKEDGNTVSYSSYEMLAGHVWRSVGKAR-GLPNDQETKLYI 300

Query: 304 TAEIRRRIRPPLPEGFVGNATLLTMTPVCKFGDLMKNPLKYAAEKIREGIWKLTDEYVES 363
             + R R+RP LP G+ GN  + T TP+   GDL+  P  YAA +I + + ++ D Y+ S
Sbjct: 301 ATDGRSRLRPQLPPGYFGN-VIFTATPLAVAGDLLSKPTWYAAGQIHDFLVRMDDNYLRS 360

Query: 364 AIDFLASKDNGWIKELYEKGTCTEGAPFWGNPNIEFGCWTSLSLYDTDFGWGRPRYVGLA 423
           A+D+L  +         +      GA  +  PN+    W  L +YD DFGWGRP ++G  
Sbjct: 361 ALDYLEMQP--------DLSALVRGAHTYKCPNLGITSWVRLPIYDADFGWGRPIFMGPG 420

Query: 424 SLENLDDGESLIMSGPEKDGCL-VVICMQTRHMDDFKRFFYQ 465
            +    +G S ++  P  DG L V I +Q+ HM  F++F ++
Sbjct: 421 GIPY--EGLSFVLPSPTNDGSLSVAIALQSEHMKLFEKFLFE 432

BLAST of Spg012973 vs. TAIR 10
Match: AT5G57840.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 211.5 bits (537), Expect = 1.5e-54
Identity = 144/480 (30.00%), Postives = 236/480 (49.17%), Query Frame = 0

Query: 4   LSLSIHSSSTILPSHPTPHRTLPLSECDQITLEGHVFTFYVYKPPNNNNNNTSSPISIES 63
           + + +  ++ + P+  TP   L LS  D I +  H+ T Y YKP ++++   +     +S
Sbjct: 1   MKIRVKQATIVKPAEETPTHNLWLSNLDLIQVRLHMGTLYFYKPCSSSDRPNT-----QS 60

Query: 64  LRTSLSQILVPYYPLAGRLRWIPRGRLELHCCAAGACFIEAEAAAKLDDHDDF-KSCDAT 123
           L  +LS++LV +YP AGRL+    GRLE+ C   G  F+EAE+ + + D     +S D  
Sbjct: 61  LIEALSKVLVFFYPAAGRLQKNTNGRLEVQCNGEGVLFVEAESDSTVQDIGLLTQSLD-- 120

Query: 124 VTNQLIPSVDYEVPIHEQPVFLVQVTRFACGGIVIGTALSHFVTDGIGAGTFINSWARIA 183
             +QL+P+VDY   I   P+ L QVT F CG I +G+++ H   +    G  + +W+  A
Sbjct: 121 -LSQLVPTVDYAGDISSYPLLLFQVTYFKCGTICVGSSIHHTFGEATSLGYIMEAWSLTA 180

Query: 184 RGESTADSVVPKPYHERDVLRVRPRMPSRFHHQEYEKPPL-------LVGHSNPEEERNR 243
           RG      V   P+ +R VL  R    S F H EY+ PP        L   SNPE     
Sbjct: 181 RGL----LVKLTPFLDRTVLHARNPPSSVFPHTEYQSPPFHNHPMKSLAYRSNPES---- 240

Query: 244 ETIVASLKLTTCQVEKLKNRANHYHSEPNSNPKPKPYTRFEVVAGHIWVCACKARKTCVT 303
           ++ +A+LKLT  Q++ LK RA    S+         ++ +EV+  H W CA  A +    
Sbjct: 241 DSAIATLKLTRLQLKALKARAEIADSK---------FSTYEVLVAHTWRCASFANEDLSE 300

Query: 304 EQPTAALVTAEIRRRIRPPLPEGFVGNATLLTMTPVCKFGDLMKNPLKYAAEKIREGIWK 363
           E  T   +  + R R++P LP+G++GN TL    PV   G   +       E++   I K
Sbjct: 301 EHSTRLHIIIDGRPRLQPKLPQGYIGN-TLFHARPVSPLGAFHRESFSETVERVHREIRK 360

Query: 364 LTDEYVESAIDFLASKDNGWIKELYEKGTCTEGAPFWGNPNIEFGCWTSLSL-------- 423
           + +EY+ SAID+L    +  + +L             G  N  F C  +  +        
Sbjct: 361 MDNEYLRSAIDYLERHPD--LDQLVP-----------GEGNTIFSCAANFCIVSLIKQTA 420

Query: 424 YDTDFGWGRPRYVGLASLENLDDGESLIMSGPEKDGCLVV-ICMQTRHMDDFKRFFYQDL 467
           Y+ DFGWG+  Y       +L++G+  +   P+++G +++ +C++   +  F++ FY+ L
Sbjct: 421 YEMDFGWGKAYY---KRASHLNEGKGFVTGNPDEEGSMMLTMCLKKTQLSKFRKLFYEFL 438

BLAST of Spg012973 vs. TAIR 10
Match: AT5G41040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 193.0 bits (489), Expect = 5.5e-49
Identity = 142/466 (30.47%), Postives = 221/466 (47.42%), Query Frame = 0

Query: 3   SLSLSIHSSSTIL--PSHPTPHRTLPLSECDQITLEGHVFTFYVYKPPNNNNNNTSSPIS 62
           ++ L +H     L  P   T      LS  DQ  +   V T Y +K     N       +
Sbjct: 28  NIHLEVHQKEPALVKPESETRKGLYFLSNLDQ-NIAVIVRTIYCFKSEERGNEE-----A 87

Query: 63  IESLRTSLSQILVPYYPLAGRLRWIPRGRLELHCCAAGACFIEAEAAAKLDDHDDFKSCD 122
           ++ ++ +LSQ+LV YYPLAGRL   P G+L + C   G  F+EAEA  K+D+  D    D
Sbjct: 88  VQVIKKALSQVLVHYYPLAGRLTISPEGKLTVDCTEEGVVFVEAEANCKMDEIGDITKPD 147

Query: 123 ATVTNQLIPSVDYEVPIHEQPVFLVQVTRFACGGIVIGTALSHFVTDGIGAGTFINSWAR 182
                +L+  V     I E P    QVT+F CGG V+G  ++H + DGIGA  F+NSW +
Sbjct: 148 PETLGKLVYDVVDAKNILEIPPVTAQVTKFKCGGFVLGLCMNHCMFDGIGAMEFVNSWGQ 207

Query: 183 IARGESTADSVVPKPYHERDVLRVRPRMPSRFHHQEYEKPPLLVGHSNPEEERNRE-TIV 242
           +ARG      +   P+ +R +L  R        HQE+E+   +   SN      +E T+ 
Sbjct: 208 VARGL----PLTTPPFSDRTILNARNPPKIENLHQEFEE---IEDKSNINSLYTKEPTLY 267

Query: 243 ASLKLTTCQVEKLKNRANHYHSEPNSNPKPKPYTRFEVVAGHIWVCACKARKTCVTEQPT 302
            S      +++KLK +A    +E + +      T FE ++  +W    K+ K  +++Q T
Sbjct: 268 RSFCFDPEKIKKLKLQA----TENSESLLGNSCTSFEALSAFVWRARTKSLK-MLSDQKT 327

Query: 303 AALVTAEIRRRIRPPLPEGFVGNATLLTMTPVCKFGDLMKNPLKYAAEKIREGIWKLTDE 362
             L   + R +  P LP+G+ GN  +LT   +C+ G+L++ PL +A   +RE I  +TD 
Sbjct: 328 KLLFAVDGRAKFEPQLPKGYFGNGIVLT-NSICEAGELIEKPLSFAVGLVREAIKMVTDG 387

Query: 363 YVESAIDFLASKDNGWIKELYEKGTCTEGAPFWGNPNIEFGCWTSLSLYDTDFGWGRPRY 422
           Y+ SAID+                  T   P   +  +    W+ L  + TDFGWG P  
Sbjct: 388 YMRSAIDYF---------------EVTRARPSLSS-TLLITTWSRLGFHTTDFGWGEPIL 447

Query: 423 VGLASLENLDDGESLIMS-GPEKDGCLVVICMQTRHMDDFKRFFYQ 465
            G  +L   +   +L +S G ++    V++ +    MD F+  F Q
Sbjct: 448 SGPVALPEKE--VTLFLSHGEQRRSINVLLGLPATAMDVFQEQFLQ 456

BLAST of Spg012973 vs. TAIR 10
Match: AT5G41040.2 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 193.0 bits (489), Expect = 5.5e-49
Identity = 142/466 (30.47%), Postives = 221/466 (47.42%), Query Frame = 0

Query: 3   SLSLSIHSSSTIL--PSHPTPHRTLPLSECDQITLEGHVFTFYVYKPPNNNNNNTSSPIS 62
           ++ L +H     L  P   T      LS  DQ  +   V T Y +K     N       +
Sbjct: 12  NIHLEVHQKEPALVKPESETRKGLYFLSNLDQ-NIAVIVRTIYCFKSEERGNEE-----A 71

Query: 63  IESLRTSLSQILVPYYPLAGRLRWIPRGRLELHCCAAGACFIEAEAAAKLDDHDDFKSCD 122
           ++ ++ +LSQ+LV YYPLAGRL   P G+L + C   G  F+EAEA  K+D+  D    D
Sbjct: 72  VQVIKKALSQVLVHYYPLAGRLTISPEGKLTVDCTEEGVVFVEAEANCKMDEIGDITKPD 131

Query: 123 ATVTNQLIPSVDYEVPIHEQPVFLVQVTRFACGGIVIGTALSHFVTDGIGAGTFINSWAR 182
                +L+  V     I E P    QVT+F CGG V+G  ++H + DGIGA  F+NSW +
Sbjct: 132 PETLGKLVYDVVDAKNILEIPPVTAQVTKFKCGGFVLGLCMNHCMFDGIGAMEFVNSWGQ 191

Query: 183 IARGESTADSVVPKPYHERDVLRVRPRMPSRFHHQEYEKPPLLVGHSNPEEERNRE-TIV 242
           +ARG      +   P+ +R +L  R        HQE+E+   +   SN      +E T+ 
Sbjct: 192 VARGL----PLTTPPFSDRTILNARNPPKIENLHQEFEE---IEDKSNINSLYTKEPTLY 251

Query: 243 ASLKLTTCQVEKLKNRANHYHSEPNSNPKPKPYTRFEVVAGHIWVCACKARKTCVTEQPT 302
            S      +++KLK +A    +E + +      T FE ++  +W    K+ K  +++Q T
Sbjct: 252 RSFCFDPEKIKKLKLQA----TENSESLLGNSCTSFEALSAFVWRARTKSLK-MLSDQKT 311

Query: 303 AALVTAEIRRRIRPPLPEGFVGNATLLTMTPVCKFGDLMKNPLKYAAEKIREGIWKLTDE 362
             L   + R +  P LP+G+ GN  +LT   +C+ G+L++ PL +A   +RE I  +TD 
Sbjct: 312 KLLFAVDGRAKFEPQLPKGYFGNGIVLT-NSICEAGELIEKPLSFAVGLVREAIKMVTDG 371

Query: 363 YVESAIDFLASKDNGWIKELYEKGTCTEGAPFWGNPNIEFGCWTSLSLYDTDFGWGRPRY 422
           Y+ SAID+                  T   P   +  +    W+ L  + TDFGWG P  
Sbjct: 372 YMRSAIDYF---------------EVTRARPSLSS-TLLITTWSRLGFHTTDFGWGEPIL 431

Query: 423 VGLASLENLDDGESLIMS-GPEKDGCLVVICMQTRHMDDFKRFFYQ 465
            G  +L   +   +L +S G ++    V++ +    MD F+  F Q
Sbjct: 432 SGPVALPEKE--VTLFLSHGEQRRSINVLLGLPATAMDVFQEQFLQ 440