Spg012914 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg012914
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionPentatricopeptide repeat-containing protein
Locationscaffold1: 6448837 .. 6451131 (+)
RNA-Seq ExpressionSpg012914
SyntenySpg012914
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CTGGCATATTCTTATTAGTTTATTAATTTAATTACCTTATTGACCAACAAGTGGATCCGTGGCGCAATGGTAGCGCGTCTGACTCCAGATCAGAAGGTTGCGTGTTCGATTCACGTCGGGTTCAATTTCCTTTTTTTTTCTTCCATTTTTTCCCAACCCATATTCATTTTATTTTTTCTCGTTCATCCTTGATCTTCCATAAATTTACAAAACCCACCCCTTTCTTCATCGGATTTTGCTATCCCCTCCCCGTCGAAGGTAACCTCAGGATGCTAATGCCCAGTTCCCCAATTCTTCTCCCACTAACATACAATGCCCCTTTCATTTTCCCTGCAATTTGCTTCCACATTCACTCTATCTTCTTCTTCCCCAAATTCCCAACTCTCTCGGCCATGGCTTCCACTCCCTTCGTCTCTGAGGAGGGACGCTGCGACCCGAGTGGTTTGCATTTCGACTCGACCCAAGAGGAAATCTGGGGCGAAAACCAACAGGTCGGAGGCTGAAGAACTGGTTCGCAGTGTAGTCAGAAAGTTCAGCGACAAAGAACCATTGCTCAAGACATTGGACAAGTACGTCCGTGTTATGAGAACTGAGCATTGCTTTCTTCTATTTGAGGAATTGGGGAAAAGGGATAAATGGCTTCAGTGCCTCGAGGTTCGTTTTTCTTCATTTGAACTCTGAATTTGGGCTCTGGGTGTTGATTTTTTAACTTCTTTAGCTGCCTGTTAAGTGTCAATAGATGGGTAATGATGAAAACATTCACAGGCAGAGTTGATCGAATTTGCAATTCGTAAACTATACACTTCCTGGTGAAATGGATGGACTTTGCACTGTGGTTCACGTTCTATTTAGCATGGATGAAGAATGGAGGGTCAAAACTTTTCTGGGTGGGGCTAAACAAGCAAAGAAATTGAATTTCTGCTTTTAACTGTGCAGAAATGTGAGAGAGATCTTGAATGTTTTGATGATGTATTTCATTTTCAGGTCTTCAGATGGATGCAGAAACAACGATGGTATATAGCTGACAATGGGGTTTATTCTAAACTAATATCTATTATGGGAAAGAAGGGCCAAATTCGGATGGCTATGTGGCTCTTCTCTGAGATGCGAAATAGTGGGTGTCGTCCTGATACTTCTGTTTACAATGCACTTATCACAGCGCATCTTCATTCTAAGGACAAAGCAAAGGCTTTGGTTAAAGTTCTTTGGTACTTTGAGAAGATGAAGGGAATGGAACGATGTAAGCCAAACATTGTTACTTACAACATTCTTACCAGAGCGTTTGCTCAAGCTGGAAAAGTGGAACAAGTTGATGCATTGTTTAAAGATCTTGATGAGAGCATTGTTTCAGCTGATATATATACATATAATGGAGTCATGGATGCATATGGGAAGATTGGGATGATCAAAGAAATGGAATTGATGCTTTCTCGCATGAAAAGCAATGAAATTAAACCAGATATCATAACCTTTAACTTGTTGATCGATTCATATGGGAAGAAACAGCTATTTGACAAGATGGAACAGGTGTTCAAGAGCTTATTACGATCAAAGGAGAGGCCGACTCTGCCTACTTTCAATTCGATGATCACGAACTACGGCAAGGCACGACTTAGAGAGAAAGCTGAAGAAGTTTTTAGGAAGATGAAGGGTATGGGATTTGACCCAAGTTATATCACCTGTGAGAGTCTAATCATGATGTATGGTCACTGTGACTGTGTTTCCAAGGCTAGAGAAATCTTCGATGGAATGGTTACTTCTGGGAAGGAGGTCAGAGTCTCAACCCTCAATTCCATGCTTGATGTTTACTGCATGAATGGTTTGCCCATGGAAGCCGATTTGCTTTTCGAGAGCGTGAGTAGTATGAAGGTGTTTCCAGATGCAACAACATACAAGCTTCTGTATAAAGCTTACACCAAAGCTGATATGAAGGAACTTCTGGAGAAGCTCTTGAAGAACATGGACAAAGCTGGCATCATTCCAAATAAGAGGTTCTTCCTCGATGCTTTGGGAACTGTTGGGTCTTCACCTGAAAAACCAGCATCTGCGAGAAATAGAACAGGTTCAAGAAACTCAGAAAGTAGTGTGGAAAAACCAGCATCTGCTTCTGCAAGAACTAGAACAGGTTCAAGAAACTCAGAAAGTAGTGTGGAAAAACCAGCATCTTCTTCTGCAAGAACTAGAACAGGTTCAAGAAACTCAGAAAGTAGTGTGGAAAAACCAGCATCTGCTTCTGCAAGAACTAGAACAGGTTCAAGAAACTTAGAAAGTAGTATGAAAAAACATGCTGCATAG

mRNA sequence

ATGCCCCTTTCATTTTCCCTGCAATTTGCTTCCACATTCACTCTATCTTCTTCTTCCCCAAATTCCCAACTCTCTCGGCCATGGCTTCCACTCCCTTCGTCTCTGAGGAGGGACGCTGCGACCCGAGTGGTTTGCATTTCGACTCGACCCAAGAGGAAATCTGGGGCGAAAACCAACAGGTCGGAGGCTGAAGAACTGGTTCGCAGTGTAGTCAGAAAGTTCAGCGACAAAGAACCATTGCTCAAGACATTGGACAAGTACGTCCGTGTTATGAGAACTGAGCATTGCTTTCTTCTATTTGAGGAATTGGGGAAAAGGGATAAATGGCTTCAGTGCCTCGAGGTCTTCAGATGGATGCAGAAACAACGATGGTATATAGCTGACAATGGGGTTTATTCTAAACTAATATCTATTATGGGAAAGAAGGGCCAAATTCGGATGGCTATGTGGCTCTTCTCTGAGATGCGAAATAGTGGGTGTCGTCCTGATACTTCTGTTTACAATGCACTTATCACAGCGCATCTTCATTCTAAGGACAAAGCAAAGGCTTTGGTTAAAGTTCTTTGGTACTTTGAGAAGATGAAGGGAATGGAACGATGTAAGCCAAACATTGTTACTTACAACATTCTTACCAGAGCGTTTGCTCAAGCTGGAAAAGTGGAACAAGTTGATGCATTGTTTAAAGATCTTGATGAGAGCATTGTTTCAGCTGATATATATACATATAATGGAGTCATGGATGCATATGGGAAGATTGGGATGATCAAAGAAATGGAATTGATGCTTTCTCGCATGAAAAGCAATGAAATTAAACCAGATATCATAACCTTTAACTTGTTGATCGATTCATATGGGAAGAAACAGCTATTTGACAAGATGGAACAGGTGTTCAAGAGCTTATTACGATCAAAGGAGAGGCCGACTCTGCCTACTTTCAATTCGATGATCACGAACTACGGCAAGGCACGACTTAGAGAGAAAGCTGAAGAAGTTTTTAGGAAGATGAAGGGTATGGGATTTGACCCAAGTTATATCACCTGTGAGAGTCTAATCATGATGTATGGTCACTGTGACTGTGTTTCCAAGGCTAGAGAAATCTTCGATGGAATGGTTACTTCTGGGAAGGAGGTCAGAGTCTCAACCCTCAATTCCATGCTTGATGTTTACTGCATGAATGGTTTGCCCATGGAAGCCGATTTGCTTTTCGAGAGCGTGAGTAGTATGAAGGTGTTTCCAGATGCAACAACATACAAGCTTCTGTATAAAGCTTACACCAAAGCTGATATGAAGGAACTTCTGGAGAAGCTCTTGAAGAACATGGACAAAGCTGGCATCATTCCAAATAAGAGGTTCTTCCTCGATGCTTTGGGAACTGTTGGGTCTTCACCTGAAAAACCAGCATCTGCGAGAAATAGAACAGGTTCAAGAAACTCAGAAAGTAGTGTGGAAAAACCAGCATCTGCTTCTGCAAGAACTAGAACAGGTTCAAGAAACTCAGAAAGTAGTGTGGAAAAACCAGCATCTTCTTCTGCAAGAACTAGAACAGGTTCAAGAAACTCAGAAAGTAGTGTGGAAAAACCAGCATCTGCTTCTGCAAGAACTAGAACAGGTTCAAGAAACTTAGAAAGTAGTATGAAAAAACATGCTGCATAG

Coding sequence (CDS)

ATGCCCCTTTCATTTTCCCTGCAATTTGCTTCCACATTCACTCTATCTTCTTCTTCCCCAAATTCCCAACTCTCTCGGCCATGGCTTCCACTCCCTTCGTCTCTGAGGAGGGACGCTGCGACCCGAGTGGTTTGCATTTCGACTCGACCCAAGAGGAAATCTGGGGCGAAAACCAACAGGTCGGAGGCTGAAGAACTGGTTCGCAGTGTAGTCAGAAAGTTCAGCGACAAAGAACCATTGCTCAAGACATTGGACAAGTACGTCCGTGTTATGAGAACTGAGCATTGCTTTCTTCTATTTGAGGAATTGGGGAAAAGGGATAAATGGCTTCAGTGCCTCGAGGTCTTCAGATGGATGCAGAAACAACGATGGTATATAGCTGACAATGGGGTTTATTCTAAACTAATATCTATTATGGGAAAGAAGGGCCAAATTCGGATGGCTATGTGGCTCTTCTCTGAGATGCGAAATAGTGGGTGTCGTCCTGATACTTCTGTTTACAATGCACTTATCACAGCGCATCTTCATTCTAAGGACAAAGCAAAGGCTTTGGTTAAAGTTCTTTGGTACTTTGAGAAGATGAAGGGAATGGAACGATGTAAGCCAAACATTGTTACTTACAACATTCTTACCAGAGCGTTTGCTCAAGCTGGAAAAGTGGAACAAGTTGATGCATTGTTTAAAGATCTTGATGAGAGCATTGTTTCAGCTGATATATATACATATAATGGAGTCATGGATGCATATGGGAAGATTGGGATGATCAAAGAAATGGAATTGATGCTTTCTCGCATGAAAAGCAATGAAATTAAACCAGATATCATAACCTTTAACTTGTTGATCGATTCATATGGGAAGAAACAGCTATTTGACAAGATGGAACAGGTGTTCAAGAGCTTATTACGATCAAAGGAGAGGCCGACTCTGCCTACTTTCAATTCGATGATCACGAACTACGGCAAGGCACGACTTAGAGAGAAAGCTGAAGAAGTTTTTAGGAAGATGAAGGGTATGGGATTTGACCCAAGTTATATCACCTGTGAGAGTCTAATCATGATGTATGGTCACTGTGACTGTGTTTCCAAGGCTAGAGAAATCTTCGATGGAATGGTTACTTCTGGGAAGGAGGTCAGAGTCTCAACCCTCAATTCCATGCTTGATGTTTACTGCATGAATGGTTTGCCCATGGAAGCCGATTTGCTTTTCGAGAGCGTGAGTAGTATGAAGGTGTTTCCAGATGCAACAACATACAAGCTTCTGTATAAAGCTTACACCAAAGCTGATATGAAGGAACTTCTGGAGAAGCTCTTGAAGAACATGGACAAAGCTGGCATCATTCCAAATAAGAGGTTCTTCCTCGATGCTTTGGGAACTGTTGGGTCTTCACCTGAAAAACCAGCATCTGCGAGAAATAGAACAGGTTCAAGAAACTCAGAAAGTAGTGTGGAAAAACCAGCATCTGCTTCTGCAAGAACTAGAACAGGTTCAAGAAACTCAGAAAGTAGTGTGGAAAAACCAGCATCTTCTTCTGCAAGAACTAGAACAGGTTCAAGAAACTCAGAAAGTAGTGTGGAAAAACCAGCATCTGCTTCTGCAAGAACTAGAACAGGTTCAAGAAACTTAGAAAGTAGTATGAAAAAACATGCTGCATAG

Protein sequence

MPLSFSLQFASTFTLSSSSPNSQLSRPWLPLPSSLRRDAATRVVCISTRPKRKSGAKTNRSEAEELVRSVVRKFSDKEPLLKTLDKYVRVMRTEHCFLLFEELGKRDKWLQCLEVFRWMQKQRWYIADNGVYSKLISIMGKKGQIRMAMWLFSEMRNSGCRPDTSVYNALITAHLHSKDKAKALVKVLWYFEKMKGMERCKPNIVTYNILTRAFAQAGKVEQVDALFKDLDESIVSADIYTYNGVMDAYGKIGMIKEMELMLSRMKSNEIKPDIITFNLLIDSYGKKQLFDKMEQVFKSLLRSKERPTLPTFNSMITNYGKARLREKAEEVFRKMKGMGFDPSYITCESLIMMYGHCDCVSKAREIFDGMVTSGKEVRVSTLNSMLDVYCMNGLPMEADLLFESVSSMKVFPDATTYKLLYKAYTKADMKELLEKLLKNMDKAGIIPNKRFFLDALGTVGSSPEKPASARNRTGSRNSESSVEKPASASARTRTGSRNSESSVEKPASSSARTRTGSRNSESSVEKPASASARTRTGSRNLESSMKKHAA
Homology
BLAST of Spg012914 vs. NCBI nr
Match: KAA0036266.1 (pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 887.9 bits (2293), Expect = 4.5e-254
Identity = 458/529 (86.58%), Postives = 489/529 (92.44%), Query Frame = 0

Query: 1   MPLSFSLQFASTFTLS-SSSPNSQLSRPWLPLPSSLRRDAATRVVCISTRPKRKSGAKTN 60
           MPLS S QF+  FT S SSS  SQLSR W P+PSSL++  +TRVVC+STRP RKSG KT+
Sbjct: 1   MPLSLSPQFSPIFTTSYSSSRTSQLSRLWFPVPSSLKKTFSTRVVCVSTRPSRKSGVKTD 60

Query: 61  RSEAEELVRSVVRKFSDKEPLLKTLDKYVRVMRTEHCFLLFEELGKRDKWLQCLEVFRWM 120
           RSEAEELVR +++KFSDKEPLLKTLDKYVRVMRTEHCFLLFEELGKRDKWLQCLEVFRWM
Sbjct: 61  RSEAEELVRGIIKKFSDKEPLLKTLDKYVRVMRTEHCFLLFEELGKRDKWLQCLEVFRWM 120

Query: 121 QKQRWYIADNGVYSKLISIMGKKGQIRMAMWLFSEMRNSGCRPDTSVYNALITAHLHSKD 180
           QKQRWYIADNGVYSKLISIMGKKGQIRMAMWLFSEMRNSGCRPDTSVYNALITAHLHSKD
Sbjct: 121 QKQRWYIADNGVYSKLISIMGKKGQIRMAMWLFSEMRNSGCRPDTSVYNALITAHLHSKD 180

Query: 181 KAKALVKVLWYFEKMKGMERCKPNIVTYNILTRAFAQAGKVEQVDALFKDLDESIVSADI 240
           KAKALVKVLWYFEKMKGMERCKPNIVTYNILTRAFAQA KV+QV+ LFKDLDES+VSADI
Sbjct: 181 KAKALVKVLWYFEKMKGMERCKPNIVTYNILTRAFAQAAKVDQVNTLFKDLDESVVSADI 240

Query: 241 YTYNGVMDAYGKIGMIKEMELMLSRMKSNEIKPDIITFNLLIDSYGKKQLFDKMEQVFKS 300
           YTYNGVMDAYGK G IKEMELML+RMKSN+IKPDII+FNLLIDSYGKKQLFDKMEQVFKS
Sbjct: 241 YTYNGVMDAYGKNGNIKEMELMLARMKSNQIKPDIISFNLLIDSYGKKQLFDKMEQVFKS 300

Query: 301 LLRSKERPTLPTFNSMITNYGKARLREKAEEVFRKMKGMGFDPSYITCESLIMMYGHCDC 360
           LLRSKERPTLPTFNSMITNYGKARLR+KAEEVFRKMK MG+DPSY+TCESLIMMYGHCDC
Sbjct: 301 LLRSKERPTLPTFNSMITNYGKARLRDKAEEVFRKMKDMGYDPSYVTCESLIMMYGHCDC 360

Query: 361 VSKAREIFDGMVTSGKEVRVSTLNSMLDVYCMNGLPMEADLLFESVSSMKVFPDATTYKL 420
           VSKAREIFDGMV SGKEVRVSTLN+MLDVYC+NGLP EAD+LFES  +MKVFPD+TTYKL
Sbjct: 361 VSKAREIFDGMVNSGKEVRVSTLNAMLDVYCINGLPFEADMLFESAGNMKVFPDSTTYKL 420

Query: 421 LYKAYTKADMKELLEKLLKNMDKAGIIPNKRFFLDALGTVGSSPEKPASARNRTGSRNSE 480
           LYKAYTKADMKELLEKLLKNMDKAGIIPNKRFFLDALGT+GSS EKP  AR +TGSRNSE
Sbjct: 421 LYKAYTKADMKELLEKLLKNMDKAGIIPNKRFFLDALGTIGSSREKPEPARTKTGSRNSE 480

Query: 481 SSVEKPASASARTRTGSRNSESSVEKPASSSARTRTGSRNSESSVEKPA 529
           SSVEK   +S+RTR+G RN ES+V+KP SS  RTRTGSRN ES VE  A
Sbjct: 481 SSVEK--QSSSRTRSGPRNLESNVQKPISS--RTRTGSRNLESGVENHA 525

BLAST of Spg012914 vs. NCBI nr
Match: XP_004143565.1 (pentatricopeptide repeat-containing protein PPR5 homolog, chloroplastic [Cucumis sativus] >KGN48788.1 hypothetical protein Csa_003022 [Cucumis sativus])

HSP 1 Score: 887.5 bits (2292), Expect = 5.9e-254
Identity = 460/528 (87.12%), Postives = 490/528 (92.80%), Query Frame = 0

Query: 1   MPLSFSLQFASTFTLSSSSPNSQLSRPWLPLPSSLRRDAATRVVCISTRPKRKSGAKTNR 60
           MPLSFSLQF+ TFT S SS  SQLSR WLPLPSS+++  +TRVVCISTRP RK G KT+R
Sbjct: 1   MPLSFSLQFSPTFTPSYSS--SQLSRLWLPLPSSIKKAVSTRVVCISTRPSRKFGVKTDR 60

Query: 61  SEAEELVRSVVRKFSDKEPLLKTLDKYVRVMRTEHCFLLFEELGKRDKWLQCLEVFRWMQ 120
           SEAEELVR ++R FSDKEPLLKTLDKYVRVMRTEHCFLLFEELGKRDKWL+CLEVFRWMQ
Sbjct: 61  SEAEELVRGIIRNFSDKEPLLKTLDKYVRVMRTEHCFLLFEELGKRDKWLECLEVFRWMQ 120

Query: 121 KQRWYIADNGVYSKLISIMGKKGQIRMAMWLFSEMRNSGCRPDTSVYNALITAHLHSKDK 180
           KQRWYIADNGVYSKLISIMGKKGQIRMAMWLFSEMRNSGCRPDTSVYNALITAHLHSKDK
Sbjct: 121 KQRWYIADNGVYSKLISIMGKKGQIRMAMWLFSEMRNSGCRPDTSVYNALITAHLHSKDK 180

Query: 181 AKALVKVLWYFEKMKGMERCKPNIVTYNILTRAFAQAGKVEQVDALFKDLDESIVSADIY 240
           AKALVKVL YFEKMKGMERCKPNIVTYNILTRAFAQA KV+QV+ LFKDLDES+VSADIY
Sbjct: 181 AKALVKVLSYFEKMKGMERCKPNIVTYNILTRAFAQAAKVDQVNTLFKDLDESVVSADIY 240

Query: 241 TYNGVMDAYGKIGMIKEMELMLSRMKSNEIKPDIITFNLLIDSYGKKQLFDKMEQVFKSL 300
           TYNGVMDAYGK G IKEMELML+RMKSN+IKPDII+FNLLIDSYGKKQLFDKMEQVFKSL
Sbjct: 241 TYNGVMDAYGKNGNIKEMELMLARMKSNQIKPDIISFNLLIDSYGKKQLFDKMEQVFKSL 300

Query: 301 LRSKERPTLPTFNSMITNYGKARLREKAEEVFRKMKGMGFDPSYITCESLIMMYGHCDCV 360
           LRSKERPTLPTFNSMITNYGKARLREKAEEVFRKMK MG+DPSY+TCESLIMMYGHCDCV
Sbjct: 301 LRSKERPTLPTFNSMITNYGKARLREKAEEVFRKMKDMGYDPSYVTCESLIMMYGHCDCV 360

Query: 361 SKAREIFDGMVTSGKEVRVSTLNSMLDVYCMNGLPMEADLLFESVSSMKVFPDATTYKLL 420
           SKAREIFDGMV SGKEVRVSTLN+MLDVYC+NGLP+EADLLFES  +M+VFPD+TTYKLL
Sbjct: 361 SKAREIFDGMVNSGKEVRVSTLNAMLDVYCINGLPLEADLLFESAGNMRVFPDSTTYKLL 420

Query: 421 YKAYTKADMKELLEKLLKNMDKAGIIPNKRFFLDALGTVGSSPEKPASARNRTGSRNSES 480
           YKAYTKAD KELLEKLLKNMDKAGIIPNKRFFLDALGT+GSS EKP  AR RTGSRNS+S
Sbjct: 421 YKAYTKADKKELLEKLLKNMDKAGIIPNKRFFLDALGTIGSSQEKPEPARTRTGSRNSDS 480

Query: 481 SVEKPASASARTRTGSRNSESSVEKPASSSARTRTGSRNSESSVEKPA 529
           SVEK   +S+RTR+G RN ES+V+KP SS  RTRTGSRNSES VE  A
Sbjct: 481 SVEK--QSSSRTRSGPRNLESNVQKPRSS--RTRTGSRNSESGVENHA 522

BLAST of Spg012914 vs. NCBI nr
Match: XP_008440667.1 (PREDICTED: pentatricopeptide repeat-containing protein At4g39620, chloroplastic [Cucumis melo] >TYK12660.1 pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 885.6 bits (2287), Expect = 2.2e-253
Identity = 458/529 (86.58%), Postives = 489/529 (92.44%), Query Frame = 0

Query: 1   MPLSFSLQFASTFTLS-SSSPNSQLSRPWLPLPSSLRRDAATRVVCISTRPKRKSGAKTN 60
           MPLS S QF+  FT S SSS  SQLSR WLP+PSSL++  +TRVVC+STR  RKSG KT+
Sbjct: 1   MPLSLSPQFSPIFTTSYSSSRTSQLSRLWLPVPSSLKKTFSTRVVCVSTRLSRKSGVKTD 60

Query: 61  RSEAEELVRSVVRKFSDKEPLLKTLDKYVRVMRTEHCFLLFEELGKRDKWLQCLEVFRWM 120
           RSEAEELVR +++KFSDKEPLLKTLDKYVRVMRTEHCFLLFEELGKRDKWLQCLEVFRWM
Sbjct: 61  RSEAEELVRGIIKKFSDKEPLLKTLDKYVRVMRTEHCFLLFEELGKRDKWLQCLEVFRWM 120

Query: 121 QKQRWYIADNGVYSKLISIMGKKGQIRMAMWLFSEMRNSGCRPDTSVYNALITAHLHSKD 180
           QKQRWYIADNGVYSKLISIMGKKGQIRMAMWLFSEMRNSGCRPDTSVYNALITAHLHSKD
Sbjct: 121 QKQRWYIADNGVYSKLISIMGKKGQIRMAMWLFSEMRNSGCRPDTSVYNALITAHLHSKD 180

Query: 181 KAKALVKVLWYFEKMKGMERCKPNIVTYNILTRAFAQAGKVEQVDALFKDLDESIVSADI 240
           KAKALVKVLWYFEKMKGMERCKPNIVTYNILTRAFAQA KV+QV+ LFKDLDES+VSADI
Sbjct: 181 KAKALVKVLWYFEKMKGMERCKPNIVTYNILTRAFAQAAKVDQVNTLFKDLDESVVSADI 240

Query: 241 YTYNGVMDAYGKIGMIKEMELMLSRMKSNEIKPDIITFNLLIDSYGKKQLFDKMEQVFKS 300
           YTYNGVMDAYGK G IKEMELML+RMKSN+IKPDII+FNLLIDSYGKKQLFDKMEQVFKS
Sbjct: 241 YTYNGVMDAYGKNGNIKEMELMLARMKSNQIKPDIISFNLLIDSYGKKQLFDKMEQVFKS 300

Query: 301 LLRSKERPTLPTFNSMITNYGKARLREKAEEVFRKMKGMGFDPSYITCESLIMMYGHCDC 360
           LLRSKERPTLPTFNSMITNYGKARLR+KAEEVFRKMK MG+DPSY+TCESLIMMYGHCDC
Sbjct: 301 LLRSKERPTLPTFNSMITNYGKARLRDKAEEVFRKMKDMGYDPSYVTCESLIMMYGHCDC 360

Query: 361 VSKAREIFDGMVTSGKEVRVSTLNSMLDVYCMNGLPMEADLLFESVSSMKVFPDATTYKL 420
           VSKAREIFDGMV SGKEVRVSTLN+MLDVYC+NGLP EAD+LFES  +MKVFPD+TTYKL
Sbjct: 361 VSKAREIFDGMVNSGKEVRVSTLNAMLDVYCINGLPFEADMLFESAGNMKVFPDSTTYKL 420

Query: 421 LYKAYTKADMKELLEKLLKNMDKAGIIPNKRFFLDALGTVGSSPEKPASARNRTGSRNSE 480
           LYKAYTKADMKELLEKLLKNMDKAGIIPNKRFFLDALGT+GSS EKP  AR +TGSRNSE
Sbjct: 421 LYKAYTKADMKELLEKLLKNMDKAGIIPNKRFFLDALGTIGSSREKPEPARTKTGSRNSE 480

Query: 481 SSVEKPASASARTRTGSRNSESSVEKPASSSARTRTGSRNSESSVEKPA 529
           SSVEK   +S+RTR+G RN ES+V+KP SS  RTRTGSRN ES VE  A
Sbjct: 481 SSVEK--QSSSRTRSGPRNLESNVQKPISS--RTRTGSRNLESGVENHA 525

BLAST of Spg012914 vs. NCBI nr
Match: XP_023543919.1 (pentatricopeptide repeat-containing protein At4g39620, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 885.2 bits (2286), Expect = 2.9e-253
Identity = 455/508 (89.57%), Postives = 477/508 (93.90%), Query Frame = 0

Query: 1   MPLSFSLQFASTFT-LSSSSPNSQLSRPWLPLPSSLRRDAATRVVCISTRPKRKSGAKTN 60
           MPLSFSLQF+ +FT  SSSS N QLS+ WLPLP SLRR  +TRVVCISTRP RKSGAKT+
Sbjct: 1   MPLSFSLQFSPSFTPFSSSSRNFQLSQTWLPLPLSLRRAVSTRVVCISTRPNRKSGAKTD 60

Query: 61  RSEAEELVRSVVRKFSDKEPLLKTLDKYVRVMRTEHCFLLFEELGKRDKWLQCLEVFRWM 120
           RSEAEELVR V+RKFSDKEPLLKTLDKYVRVMRTEHCFLLFEELGK+DKWLQCLEVFRWM
Sbjct: 61  RSEAEELVRGVIRKFSDKEPLLKTLDKYVRVMRTEHCFLLFEELGKKDKWLQCLEVFRWM 120

Query: 121 QKQRWYIADNGVYSKLISIMGKKGQIRMAMWLFSEMRNSGCRPDTSVYNALITAHLHSKD 180
           QKQRWYIADNGVYSKLISIMGKKGQIRMA+WLFSEMRNSGCRPDTSVYNALITAHLHSKD
Sbjct: 121 QKQRWYIADNGVYSKLISIMGKKGQIRMAIWLFSEMRNSGCRPDTSVYNALITAHLHSKD 180

Query: 181 KAKALVKVLWYFEKMKGMERCKPNIVTYNILTRAFAQAGKVEQVDALFKDLDESIVSADI 240
           KAKALVKVLWYFEKMKGMERCKPN+VTYNILTRAFAQAGKV+QV+ALFKDLDESIVSADI
Sbjct: 181 KAKALVKVLWYFEKMKGMERCKPNVVTYNILTRAFAQAGKVDQVNALFKDLDESIVSADI 240

Query: 241 YTYNGVMDAYGKIGMIKEMELMLSRMKSNEIKPDIITFNLLIDSYGKKQLFDKMEQVFKS 300
           YTYNGVMDAYGK GMIKEME ML+RMKSNEIKPDII+FNLLIDSYGKKQLFDKMEQVFKS
Sbjct: 241 YTYNGVMDAYGKNGMIKEMETMLARMKSNEIKPDIISFNLLIDSYGKKQLFDKMEQVFKS 300

Query: 301 LLRSKERPTLPTFNSMITNYGKARLREKAEEVFRKMKGMGFDPSYITCESLIMMYGHCDC 360
           LLRSKERPTLPTFNSMITNYGKARLRE+AEEVFRKMK MG+DPSY+TCESLIMMYGHCDC
Sbjct: 301 LLRSKERPTLPTFNSMITNYGKARLRERAEEVFRKMKDMGYDPSYVTCESLIMMYGHCDC 360

Query: 361 VSKAREIFDGMVTSGKEVRVSTLNSMLDVYCMNGLPMEADLLFESVSSMKVFPDATTYKL 420
           VSKAREIFDGMV SGK VRVSTLN+MLDVYCMNGLPMEADLLFES SSMKVFPD+TTYKL
Sbjct: 361 VSKAREIFDGMVNSGKAVRVSTLNAMLDVYCMNGLPMEADLLFESASSMKVFPDSTTYKL 420

Query: 421 LYKAYTKADMKELLEKLLKNMDKAGIIPNKRFFLDALGTVGSSPEKPASARNRTGSRNSE 480
           LYKAYTKAD KEL+EKLLKNMDKAGIIPNKRFFLDALGTVGSSPEKP  AR  TGSR+SE
Sbjct: 421 LYKAYTKADAKELVEKLLKNMDKAGIIPNKRFFLDALGTVGSSPEKPEPARTGTGSRHSE 480

Query: 481 SSVEKPASASARTRTGSRNSESSVEKPA 508
           SSVEKP   S+R R GSR  ESSV+  A
Sbjct: 481 SSVEKP--ISSRNRRGSRKLESSVKNHA 506

BLAST of Spg012914 vs. NCBI nr
Match: KAG7034169.1 (Pentatricopeptide repeat-containing protein, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 884.0 bits (2283), Expect = 6.5e-253
Identity = 455/508 (89.57%), Postives = 476/508 (93.70%), Query Frame = 0

Query: 1   MPLSFSLQFASTFT-LSSSSPNSQLSRPWLPLPSSLRRDAATRVVCISTRPKRKSGAKTN 60
           MPLSFSLQF+ +FT  SSSS N QLS+ WLPLP SLRR  +TRVVCISTRP RKS AKT+
Sbjct: 1   MPLSFSLQFSPSFTPFSSSSRNFQLSQTWLPLPLSLRRAVSTRVVCISTRPNRKSAAKTD 60

Query: 61  RSEAEELVRSVVRKFSDKEPLLKTLDKYVRVMRTEHCFLLFEELGKRDKWLQCLEVFRWM 120
           RSEAEELVR V+RKFSDKEPLLKTLDKYVRVMRTEHCFLLFEELGK+DKWLQCLEVFRWM
Sbjct: 61  RSEAEELVRGVIRKFSDKEPLLKTLDKYVRVMRTEHCFLLFEELGKKDKWLQCLEVFRWM 120

Query: 121 QKQRWYIADNGVYSKLISIMGKKGQIRMAMWLFSEMRNSGCRPDTSVYNALITAHLHSKD 180
           QKQRWYIADNGVYSKLISIMGKKGQIRMA+WLFSEMRNSGCRPDTSVYNALITAHLHSKD
Sbjct: 121 QKQRWYIADNGVYSKLISIMGKKGQIRMAIWLFSEMRNSGCRPDTSVYNALITAHLHSKD 180

Query: 181 KAKALVKVLWYFEKMKGMERCKPNIVTYNILTRAFAQAGKVEQVDALFKDLDESIVSADI 240
           KAKALVKVLWYFEKMKGMERCKPN+VTYNILTRAFAQAGKV+QV+ALFKDLDESIVSADI
Sbjct: 181 KAKALVKVLWYFEKMKGMERCKPNVVTYNILTRAFAQAGKVDQVNALFKDLDESIVSADI 240

Query: 241 YTYNGVMDAYGKIGMIKEMELMLSRMKSNEIKPDIITFNLLIDSYGKKQLFDKMEQVFKS 300
           YTYNGVMDAYGK GMIKEME ML+RMKSNEIKPDII+FNLLIDSYGKKQLFDKMEQVFKS
Sbjct: 241 YTYNGVMDAYGKNGMIKEMETMLARMKSNEIKPDIISFNLLIDSYGKKQLFDKMEQVFKS 300

Query: 301 LLRSKERPTLPTFNSMITNYGKARLREKAEEVFRKMKGMGFDPSYITCESLIMMYGHCDC 360
           LLRSKERPTLPTFNSMITNYGKARLREKAEEVFRKMK MG+DPSY+TCESLIMMYGHCDC
Sbjct: 301 LLRSKERPTLPTFNSMITNYGKARLREKAEEVFRKMKDMGYDPSYVTCESLIMMYGHCDC 360

Query: 361 VSKAREIFDGMVTSGKEVRVSTLNSMLDVYCMNGLPMEADLLFESVSSMKVFPDATTYKL 420
           VSKAREIFDGMV SGK VRVSTLN+MLDVYCMNGLPMEADLLFES SSMKVFPD+TTYKL
Sbjct: 361 VSKAREIFDGMVNSGKAVRVSTLNAMLDVYCMNGLPMEADLLFESASSMKVFPDSTTYKL 420

Query: 421 LYKAYTKADMKELLEKLLKNMDKAGIIPNKRFFLDALGTVGSSPEKPASARNRTGSRNSE 480
           LYKAYTKAD KEL+EKLLKNMDKAGIIPNKRFFLDALGTVGSSPEKP  AR  TGSR+SE
Sbjct: 421 LYKAYTKADAKELVEKLLKNMDKAGIIPNKRFFLDALGTVGSSPEKPEPARTGTGSRHSE 480

Query: 481 SSVEKPASASARTRTGSRNSESSVEKPA 508
           SSVEKP   S+R R GSR  ESSV+  A
Sbjct: 481 SSVEKP--ISSRNRRGSRKLESSVKNHA 506

BLAST of Spg012914 vs. ExPASy Swiss-Prot
Match: Q9SV96 (Pentatricopeptide repeat-containing protein At4g39620, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=EMB2453 PE=2 SV=1)

HSP 1 Score: 602.8 bits (1553), Expect = 3.8e-171
Identity = 318/491 (64.77%), Postives = 385/491 (78.41%), Query Frame = 0

Query: 18  SSPNSQLSRPWLPLPSSL---RRDAATRVVC--ISTRPK---RKSGAKTNRSEAEELVRS 77
           SS   +    WL    +L   RR   TR+ C  IS+R K   R+S  + NR     LVRS
Sbjct: 18  SSIPKETDHKWLRFSVNLGDARRSTRTRITCGAISSRRKLAERESAERENR----VLVRS 77

Query: 78  VVRKFSDKEPLLKTLDKYVRVMRTEHCFLLFEELGKRDKWLQCLEVFRWMQKQRWYIADN 137
           ++ + SD+EPL+KTLDKYV+V+R +HCFLLFEELGK DKWLQCLEVFRWMQKQRWYI DN
Sbjct: 78  LMSRISDREPLVKTLDKYVKVVRCDHCFLLFEELGKSDKWLQCLEVFRWMQKQRWYIPDN 137

Query: 138 GVYSKLISIMGKKGQIRMAMWLFSEMRNSGCRPDTSVYNALITAHLHSKDKAKALVKVLW 197
           GVYSKLIS+MGKKGQ RMAMWLFSEM+NSGCRPD SVYNALITAHLH++DKAKAL KV  
Sbjct: 138 GVYSKLISVMGKKGQTRMAMWLFSEMKNSGCRPDASVYNALITAHLHTRDKAKALEKVRG 197

Query: 198 YFEKMKGMERCKPNIVTYNILTRAFAQAGKVEQVDALFKDLDESIVSADIYTYNGVMDAY 257
           Y +KMKG+ERC+PN+VTYNIL RAFAQ+GKV+QV+ALFKDLD S VS D+YT+NGVMDAY
Sbjct: 198 YLDKMKGIERCQPNVVTYNILLRAFAQSGKVDQVNALFKDLDMSPVSPDVYTFNGVMDAY 257

Query: 258 GKIGMIKEMELMLSRMKSNEIKPDIITFNLLIDSYGKKQLFDKMEQVFKSLLRSKERPTL 317
           GK GMIKEME +L+RM+SNE KPDIITFN+LIDSYGKKQ F+KMEQ FKSL+RSKE+PTL
Sbjct: 258 GKNGMIKEMEAVLTRMRSNECKPDIITFNVLIDSYGKKQEFEKMEQTFKSLMRSKEKPTL 317

Query: 318 PTFNSMITNYGKARLREKAEEVFRKMKGMGFDPSYITCESLIMMYGHCDCVSKAREIFDG 377
           PTFNSMI NYGKAR+ +KAE VF+KM  M + PS+IT E +IMMYG+C  VS+AREIF+ 
Sbjct: 318 PTFNSMIINYGKARMIDKAEWVFKKMNDMNYIPSFITYECMIMMYGYCGSVSRAREIFEE 377

Query: 378 MVTSGKEVRVSTLNSMLDVYCMNGLPMEADLLFESVSSMKVFPDATTYKLLYKAYTKADM 437
           +  S + ++ STLN+ML+VYC NGL +EAD LF + S+ +V PDA+TYK LYKAYTKADM
Sbjct: 378 VGESDRVLKASTLNAMLEVYCRNGLYIEADKLFHNASAFRVHPDASTYKFLYKAYTKADM 437

Query: 438 KELLEKLLKNMDKAGIIPNKRFFLDALGTVGSSPEKPASARNRTGSRNSESSVEKPASAS 497
           KE ++ L+K M+K GI+PNKRFFL+AL   GS    P S     GS N +S+    +  S
Sbjct: 438 KEQVQILMKKMEKDGIVPNKRFFLEALEVFGS--RLPGS-----GSENRKSTRSSRSRDS 497

Query: 498 ARTRTGSRNSE 501
            + R G++ +E
Sbjct: 498 PKGRGGNQLTE 497

BLAST of Spg012914 vs. ExPASy Swiss-Prot
Match: A7LN87 (Pentatricopeptide repeat-containing protein PPR5, chloroplastic OS=Zea mays OX=4577 GN=PPR5 PE=2 SV=1)

HSP 1 Score: 600.5 bits (1547), Expect = 1.9e-170
Identity = 313/490 (63.88%), Postives = 379/490 (77.35%), Query Frame = 0

Query: 16  SSSSPNSQLSRPWLPLPSSLRRDAATRVVCISTRPKRK-------SGAKTNRSEAEELVR 75
           S+SSP  Q  +P  P P       ATR V ++ R KR+        G     +EA ELVR
Sbjct: 6   STSSPWPQ-RQPPSPCPGG--GGGATRHVALAARSKRRGAGPAAAEGVDEAAAEAAELVR 65

Query: 76  SVVRKFS-DKEPLLKTLDKYVRVMRTEHCFLLFEELGKRDKWLQCLEVFRWMQKQRWYIA 135
           S++R+ +  KE L+  LD++VRV+RTEHCFLLFEELG+RD WLQCL+VFRWMQKQRWY+A
Sbjct: 66  SLLRRTAGGKERLVPVLDRHVRVVRTEHCFLLFEELGRRDAWLQCLDVFRWMQKQRWYVA 125

Query: 136 DNGVYSKLISIMGKKGQIRMAMWLFSEMRNSGCRPDTSVYNALITAHLHSKDKAKALVKV 195
           DNG+YSKLIS+MG+KGQIRMAMWLFS+MRNSGC+PDTSVYN+LI AHLHS+DK KAL K 
Sbjct: 126 DNGIYSKLISVMGRKGQIRMAMWLFSQMRNSGCKPDTSVYNSLIGAHLHSRDKTKALAKA 185

Query: 196 LWYFEKMKGMERCKPNIVTYNILTRAFAQAGKVEQVDALFKDLDESIVSADIYTYNGVMD 255
           L YFEKMK +ERC+P IVTYNIL RAFAQAG  +QVD LFKDLDES+VS D+YTYNGV+D
Sbjct: 186 LGYFEKMKCIERCQPTIVTYNILLRAFAQAGDTKQVDMLFKDLDESVVSPDVYTYNGVLD 245

Query: 256 AYGKIGMIKEMELMLSRMKSNEIKPDIITFNLLIDSYGKKQLFDKMEQVFKSLLRSKERP 315
           AYGK GMIKEME +L RMKS + +PD+ITFN+LIDSYG+KQ FDKMEQVFKSLLRSKERP
Sbjct: 246 AYGKNGMIKEMESVLVRMKSTQCRPDVITFNILIDSYGRKQTFDKMEQVFKSLLRSKERP 305

Query: 316 TLPTFNSMITNYGKARLREKAEEVFRKMKGMGFDPSYITCESLIMMYGHCDCVSKAREIF 375
           T PTFNSMITNYG+ARLREKAE V  KM+ +GF P+Y+T E LI+MY HCDCVSKAR++F
Sbjct: 306 THPTFNSMITNYGRARLREKAESVVEKMEELGFKPNYVTQECLIIMYAHCDCVSKARQVF 365

Query: 376 DGMVTSGKEVRVSTLNSMLDVYCMNGLPMEADLLFESVSSMKVFPDATTYKLLYKAYTKA 435
           D +VTS  +V +S+LNSML+ YCMNGL  EAD L ++     V P+ +TYKLLYKAYTKA
Sbjct: 366 DELVTSQTKVHLSSLNSMLEAYCMNGLHTEADRLLDTALQQCVVPNGSTYKLLYKAYTKA 425

Query: 436 DMKELLEKLLKNMDKAGIIPNKRFFLDALGTVGSSPEKPASARNRTGSRNSESSVEKPAS 495
           + K L++KLLK M+K GI+PNK+FFLDAL   G+S  KP +    +   NS S     ++
Sbjct: 426 NDKLLVQKLLKRMNKQGIVPNKKFFLDALEAFGTSDRKPRT----SPGINSASKPSTDSA 485

Query: 496 ASARTRTGSR 498
             + T T  +
Sbjct: 486 GDSETATSDK 488

BLAST of Spg012914 vs. ExPASy Swiss-Prot
Match: A3ABE1 (Pentatricopeptide repeat-containing protein PPR5 homolog, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0750400 PE=3 SV=2)

HSP 1 Score: 597.0 bits (1538), Expect = 2.1e-169
Identity = 311/482 (64.52%), Postives = 376/482 (78.01%), Query Frame = 0

Query: 25  SRPWLPLPSSLRRDAATR--VVCISTRPKRK-SGAKT-----NRSEAEELVRSVVRKFS- 84
           S PW P         A R  +   + R KR+ +GA         +EA +LVR  +R+ S 
Sbjct: 8   SSPWPPRHHGAAAAPAARRHMAAAAARGKRRGAGAAAAEGADEAAEAADLVRFFLRRTSG 67

Query: 85  DKEPLLKTLDKYVRVMRTEHCFLLFEELGKRDKWLQCLEVFRWMQKQRWYIADNGVYSKL 144
            KE L+  LD++V+V+RTEHCFLLFEELG+RD WLQCLEVFRWMQKQRWY+ADNG+YSKL
Sbjct: 68  GKERLVAVLDRHVKVVRTEHCFLLFEELGRRDGWLQCLEVFRWMQKQRWYVADNGIYSKL 127

Query: 145 ISIMGKKGQIRMAMWLFSEMRNSGCRPDTSVYNALITAHLHSKDKAKALVKVLWYFEKMK 204
           IS+MG+KGQIRMAMWLFS+MRNSGCRPDTSVYN+LI  HLHS+DK+KAL K L YFEKMK
Sbjct: 128 ISVMGRKGQIRMAMWLFSQMRNSGCRPDTSVYNSLIGTHLHSRDKSKALAKALGYFEKMK 187

Query: 205 GMERCKPNIVTYNILTRAFAQAGKVEQVDALFKDLDESIVSADIYTYNGVMDAYGKIGMI 264
            ++RC+PNIVTYNIL RAFAQAG  +Q+D LFKDLDES VS DIYTYNGVMDAYGK GMI
Sbjct: 188 TIDRCQPNIVTYNILLRAFAQAGDTKQLDILFKDLDESPVSPDIYTYNGVMDAYGKNGMI 247

Query: 265 KEMELMLSRMKSNEIKPDIITFNLLIDSYGKKQLFDKMEQVFKSLLRSKERPTLPTFNSM 324
            EME +L RMKSN+ +PD+ITFN+LIDSYG+KQ FDKMEQVFKSLLRSKE+PT PTFNSM
Sbjct: 248 TEMESVLVRMKSNQCRPDVITFNILIDSYGRKQAFDKMEQVFKSLLRSKEKPTHPTFNSM 307

Query: 325 ITNYGKARLREKAEEVFRKMKGMGFDPSYITCESLIMMYGHCDCVSKAREIFDGMVTSGK 384
           ITNYGKARLREKAE V  KM  MGF P+Y+T E LIMMY +CDCVS+AR+IFD +V+S  
Sbjct: 308 ITNYGKARLREKAECVLDKMTEMGFKPNYVTQECLIMMYAYCDCVSRARQIFDELVSSQN 367

Query: 385 EVRVSTLNSMLDVYCMNGLPMEADLLFESVSSMKVFPDATTYKLLYKAYTKADMKELLEK 444
            V +S++N+MLD YCMNGLPMEAD L +SV      P A+TYKLLYKAYTKA+ K+L++K
Sbjct: 368 NVHLSSVNAMLDAYCMNGLPMEADQLLDSVIKKGAVPSASTYKLLYKAYTKANDKKLIQK 427

Query: 445 LLKNMDKAGIIPNKRFFLDALGTVGSSPEKPASARNRTGSRNSESSVEKPASASARTRTG 498
           LLK M+  GI+PNK+FFLDAL   G++ +KP +      S+NS S  +  ++ ++ T T 
Sbjct: 428 LLKRMNSQGIVPNKKFFLDALEAFGNTDKKPRT----VPSKNSASKPDVESANNSGTDTS 485

BLAST of Spg012914 vs. ExPASy Swiss-Prot
Match: Q9SQU6 (Pentatricopeptide repeat-containing protein At3g06430, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=EMB2750 PE=1 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 5.4e-40
Identity = 101/354 (28.53%), Postives = 174/354 (49.15%), Query Frame = 0

Query: 102 ELGKRDKWLQCLEVFRWMQKQRWYIADNGVYSKLISIMGKKGQIRMAMWLFSEMRNSGCR 161
           +L  + +WLQ LEVF  +++Q +Y    G Y KL+ ++GK GQ   A  LF EM   G  
Sbjct: 97  DLIAKKQWLQALEVFDMLREQTFYQPKEGTYMKLLVLLGKSGQPNRAQKLFDEMLEEGLE 156

Query: 162 PDTSVYNALITAHLHSK--DKAKALVKVLWYFEKMKGMERCKPNIVTYNILTRAFAQAGK 221
           P   +Y AL+ A+  S   D A +++      +KMK   +C+P++ TY+ L +A   A +
Sbjct: 157 PTVELYTALLAAYTRSNLIDDAFSIL------DKMKSFPQCQPDVFTYSTLLKACVDASQ 216

Query: 222 VEQVDALFKDLDESIVSADIYTYNGVMDAYGKIGMIKEMELMLSRM-KSNEIKPDIITFN 281
            + VD+L+K++DE +++ +  T N V+  YG++G   +ME +LS M  S   KPD+ T N
Sbjct: 217 FDLVDSLYKEMDERLITPNTVTQNIVLSGYGRVGRFDQMEKVLSDMLVSTACKPDVWTMN 276

Query: 282 LLIDSYGKKQLFDKMEQVFKSLLRSKERPTLPTFNSMITNYGKARLREKAEEVFRKMKGM 341
           +++  +G     D ME  ++        P   TFN +I +YGK R+ +K   V   M+ +
Sbjct: 277 IILSVFGNMGKIDMMESWYEKFRNFGIEPETRTFNILIGSYGKKRMYDKMSSVMEYMRKL 336

Query: 342 GFDPSYITCESLIMMYGHCDCVSKAREIFDGMVTSGKEVRVSTLNSMLDVYCMNGLPMEA 401
            F  +  T  ++I  +            FD M + G +    T   +++ Y   GL  + 
Sbjct: 337 EFPWTTSTYNNIIEAFADVGDAKNMELTFDQMRSEGMKADTKTFCCLINGYANAGLFHKV 396

Query: 402 DLLFESVSSMKVFPDATTYKLLYKAYTKADMKELLEKLLKNMDKAGIIPNKRFF 453
               +  +  ++  +   Y  +  A  KAD    +E++   M +   + + R F
Sbjct: 397 ISSVQLAAKFEIPENTAFYNAVISACAKADDLIEMERVYIRMKERQCVCDSRTF 444

BLAST of Spg012914 vs. ExPASy Swiss-Prot
Match: Q9SCP4 (Pentatricopeptide repeat-containing protein At3g53170 OS=Arabidopsis thaliana OX=3702 GN=At3g53170 PE=3 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 1.0e-38
Identity = 108/380 (28.42%), Postives = 173/380 (45.53%), Query Frame = 0

Query: 101 EELGKRDKWLQCLEVFRWMQKQRWYIADNGVYSKLISIMGKKGQIRMAMWLFSEMRNSGC 160
           +E  K ++W   L++F  ++KQ WY      Y+KL  ++G   Q   A  LF  M + G 
Sbjct: 66  DEAIKENRWQSALKIFNLLRKQHWYEPRCKTYTKLFKVLGNCKQPDQASLLFEVMLSEGL 125

Query: 161 RPDTSVYNALITAHLHSKDKAKALVKVLWYFEKMKGMERCKPNIVTYNILTRAFAQAGKV 220
           +P   VY +LI+ +     K++ L K     E MK +  CKP++ T+ +L     + G+ 
Sbjct: 126 KPTIDVYTSLISVY----GKSELLDKAFSTLEYMKSVSDCKPDVFTFTVLISCCCKLGRF 185

Query: 221 EQVDALFKDLDESIVSADIYTYNGVMDAYGKIGMIKEMELML------------------ 280
           + V ++  ++    V     TYN ++D YGK GM +EME +L                  
Sbjct: 186 DLVKSIVLEMSYLGVGCSTVTYNTIIDGYGKAGMFEEMESVLADMIEDGDSLPDVCTLNS 245

Query: 281 ------------------SRMKSNEIKPDIITFNLLIDSYGKKQLFDKMEQVFKSLLRSK 340
                             SR +   ++PDI TFN+LI S+GK  ++ KM  V   + +  
Sbjct: 246 IIGSYGNGRNMRKMESWYSRFQLMGVQPDITTFNILILSFGKAGMYKKMCSVMDFMEKRF 305

Query: 341 ERPTLPTFNSMITNYGKARLREKAEEVFRKMKGMGFDPSYITCESLIMMYGHCDCVSKAR 400
              T  T+N +I  +GKA   EK ++VFRKMK  G  P+ IT  SL+  Y     V K  
Sbjct: 306 FSLTTVTYNIVIETFGKAGRIEKMDDVFRKMKYQGVKPNSITYCSLVNAYSKAGLVVKID 365

Query: 401 EIFDGMVTSGKEVRVSTLNSMLDVYCMNGLPMEADLLFESVSSMKVFPDATTYKLLYKAY 445
            +   +V S   +     N +++ Y   G       L+  +   K  PD  T+  + K Y
Sbjct: 366 SVLRQIVNSDVVLDTPFFNCIINAYGQAGDLATMKELYIQMEERKCKPDKITFATMIKTY 425

BLAST of Spg012914 vs. ExPASy TrEMBL
Match: A0A5A7T266 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold18G00650 PE=3 SV=1)

HSP 1 Score: 887.9 bits (2293), Expect = 2.2e-254
Identity = 458/529 (86.58%), Postives = 489/529 (92.44%), Query Frame = 0

Query: 1   MPLSFSLQFASTFTLS-SSSPNSQLSRPWLPLPSSLRRDAATRVVCISTRPKRKSGAKTN 60
           MPLS S QF+  FT S SSS  SQLSR W P+PSSL++  +TRVVC+STRP RKSG KT+
Sbjct: 1   MPLSLSPQFSPIFTTSYSSSRTSQLSRLWFPVPSSLKKTFSTRVVCVSTRPSRKSGVKTD 60

Query: 61  RSEAEELVRSVVRKFSDKEPLLKTLDKYVRVMRTEHCFLLFEELGKRDKWLQCLEVFRWM 120
           RSEAEELVR +++KFSDKEPLLKTLDKYVRVMRTEHCFLLFEELGKRDKWLQCLEVFRWM
Sbjct: 61  RSEAEELVRGIIKKFSDKEPLLKTLDKYVRVMRTEHCFLLFEELGKRDKWLQCLEVFRWM 120

Query: 121 QKQRWYIADNGVYSKLISIMGKKGQIRMAMWLFSEMRNSGCRPDTSVYNALITAHLHSKD 180
           QKQRWYIADNGVYSKLISIMGKKGQIRMAMWLFSEMRNSGCRPDTSVYNALITAHLHSKD
Sbjct: 121 QKQRWYIADNGVYSKLISIMGKKGQIRMAMWLFSEMRNSGCRPDTSVYNALITAHLHSKD 180

Query: 181 KAKALVKVLWYFEKMKGMERCKPNIVTYNILTRAFAQAGKVEQVDALFKDLDESIVSADI 240
           KAKALVKVLWYFEKMKGMERCKPNIVTYNILTRAFAQA KV+QV+ LFKDLDES+VSADI
Sbjct: 181 KAKALVKVLWYFEKMKGMERCKPNIVTYNILTRAFAQAAKVDQVNTLFKDLDESVVSADI 240

Query: 241 YTYNGVMDAYGKIGMIKEMELMLSRMKSNEIKPDIITFNLLIDSYGKKQLFDKMEQVFKS 300
           YTYNGVMDAYGK G IKEMELML+RMKSN+IKPDII+FNLLIDSYGKKQLFDKMEQVFKS
Sbjct: 241 YTYNGVMDAYGKNGNIKEMELMLARMKSNQIKPDIISFNLLIDSYGKKQLFDKMEQVFKS 300

Query: 301 LLRSKERPTLPTFNSMITNYGKARLREKAEEVFRKMKGMGFDPSYITCESLIMMYGHCDC 360
           LLRSKERPTLPTFNSMITNYGKARLR+KAEEVFRKMK MG+DPSY+TCESLIMMYGHCDC
Sbjct: 301 LLRSKERPTLPTFNSMITNYGKARLRDKAEEVFRKMKDMGYDPSYVTCESLIMMYGHCDC 360

Query: 361 VSKAREIFDGMVTSGKEVRVSTLNSMLDVYCMNGLPMEADLLFESVSSMKVFPDATTYKL 420
           VSKAREIFDGMV SGKEVRVSTLN+MLDVYC+NGLP EAD+LFES  +MKVFPD+TTYKL
Sbjct: 361 VSKAREIFDGMVNSGKEVRVSTLNAMLDVYCINGLPFEADMLFESAGNMKVFPDSTTYKL 420

Query: 421 LYKAYTKADMKELLEKLLKNMDKAGIIPNKRFFLDALGTVGSSPEKPASARNRTGSRNSE 480
           LYKAYTKADMKELLEKLLKNMDKAGIIPNKRFFLDALGT+GSS EKP  AR +TGSRNSE
Sbjct: 421 LYKAYTKADMKELLEKLLKNMDKAGIIPNKRFFLDALGTIGSSREKPEPARTKTGSRNSE 480

Query: 481 SSVEKPASASARTRTGSRNSESSVEKPASSSARTRTGSRNSESSVEKPA 529
           SSVEK   +S+RTR+G RN ES+V+KP SS  RTRTGSRN ES VE  A
Sbjct: 481 SSVEK--QSSSRTRSGPRNLESNVQKPISS--RTRTGSRNLESGVENHA 525

BLAST of Spg012914 vs. ExPASy TrEMBL
Match: A0A0A0KGV8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G501290 PE=3 SV=1)

HSP 1 Score: 887.5 bits (2292), Expect = 2.9e-254
Identity = 460/528 (87.12%), Postives = 490/528 (92.80%), Query Frame = 0

Query: 1   MPLSFSLQFASTFTLSSSSPNSQLSRPWLPLPSSLRRDAATRVVCISTRPKRKSGAKTNR 60
           MPLSFSLQF+ TFT S SS  SQLSR WLPLPSS+++  +TRVVCISTRP RK G KT+R
Sbjct: 1   MPLSFSLQFSPTFTPSYSS--SQLSRLWLPLPSSIKKAVSTRVVCISTRPSRKFGVKTDR 60

Query: 61  SEAEELVRSVVRKFSDKEPLLKTLDKYVRVMRTEHCFLLFEELGKRDKWLQCLEVFRWMQ 120
           SEAEELVR ++R FSDKEPLLKTLDKYVRVMRTEHCFLLFEELGKRDKWL+CLEVFRWMQ
Sbjct: 61  SEAEELVRGIIRNFSDKEPLLKTLDKYVRVMRTEHCFLLFEELGKRDKWLECLEVFRWMQ 120

Query: 121 KQRWYIADNGVYSKLISIMGKKGQIRMAMWLFSEMRNSGCRPDTSVYNALITAHLHSKDK 180
           KQRWYIADNGVYSKLISIMGKKGQIRMAMWLFSEMRNSGCRPDTSVYNALITAHLHSKDK
Sbjct: 121 KQRWYIADNGVYSKLISIMGKKGQIRMAMWLFSEMRNSGCRPDTSVYNALITAHLHSKDK 180

Query: 181 AKALVKVLWYFEKMKGMERCKPNIVTYNILTRAFAQAGKVEQVDALFKDLDESIVSADIY 240
           AKALVKVL YFEKMKGMERCKPNIVTYNILTRAFAQA KV+QV+ LFKDLDES+VSADIY
Sbjct: 181 AKALVKVLSYFEKMKGMERCKPNIVTYNILTRAFAQAAKVDQVNTLFKDLDESVVSADIY 240

Query: 241 TYNGVMDAYGKIGMIKEMELMLSRMKSNEIKPDIITFNLLIDSYGKKQLFDKMEQVFKSL 300
           TYNGVMDAYGK G IKEMELML+RMKSN+IKPDII+FNLLIDSYGKKQLFDKMEQVFKSL
Sbjct: 241 TYNGVMDAYGKNGNIKEMELMLARMKSNQIKPDIISFNLLIDSYGKKQLFDKMEQVFKSL 300

Query: 301 LRSKERPTLPTFNSMITNYGKARLREKAEEVFRKMKGMGFDPSYITCESLIMMYGHCDCV 360
           LRSKERPTLPTFNSMITNYGKARLREKAEEVFRKMK MG+DPSY+TCESLIMMYGHCDCV
Sbjct: 301 LRSKERPTLPTFNSMITNYGKARLREKAEEVFRKMKDMGYDPSYVTCESLIMMYGHCDCV 360

Query: 361 SKAREIFDGMVTSGKEVRVSTLNSMLDVYCMNGLPMEADLLFESVSSMKVFPDATTYKLL 420
           SKAREIFDGMV SGKEVRVSTLN+MLDVYC+NGLP+EADLLFES  +M+VFPD+TTYKLL
Sbjct: 361 SKAREIFDGMVNSGKEVRVSTLNAMLDVYCINGLPLEADLLFESAGNMRVFPDSTTYKLL 420

Query: 421 YKAYTKADMKELLEKLLKNMDKAGIIPNKRFFLDALGTVGSSPEKPASARNRTGSRNSES 480
           YKAYTKAD KELLEKLLKNMDKAGIIPNKRFFLDALGT+GSS EKP  AR RTGSRNS+S
Sbjct: 421 YKAYTKADKKELLEKLLKNMDKAGIIPNKRFFLDALGTIGSSQEKPEPARTRTGSRNSDS 480

Query: 481 SVEKPASASARTRTGSRNSESSVEKPASSSARTRTGSRNSESSVEKPA 529
           SVEK   +S+RTR+G RN ES+V+KP SS  RTRTGSRNSES VE  A
Sbjct: 481 SVEK--QSSSRTRSGPRNLESNVQKPRSS--RTRTGSRNSESGVENHA 522

BLAST of Spg012914 vs. ExPASy TrEMBL
Match: A0A5D3CMK0 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002480 PE=3 SV=1)

HSP 1 Score: 885.6 bits (2287), Expect = 1.1e-253
Identity = 458/529 (86.58%), Postives = 489/529 (92.44%), Query Frame = 0

Query: 1   MPLSFSLQFASTFTLS-SSSPNSQLSRPWLPLPSSLRRDAATRVVCISTRPKRKSGAKTN 60
           MPLS S QF+  FT S SSS  SQLSR WLP+PSSL++  +TRVVC+STR  RKSG KT+
Sbjct: 1   MPLSLSPQFSPIFTTSYSSSRTSQLSRLWLPVPSSLKKTFSTRVVCVSTRLSRKSGVKTD 60

Query: 61  RSEAEELVRSVVRKFSDKEPLLKTLDKYVRVMRTEHCFLLFEELGKRDKWLQCLEVFRWM 120
           RSEAEELVR +++KFSDKEPLLKTLDKYVRVMRTEHCFLLFEELGKRDKWLQCLEVFRWM
Sbjct: 61  RSEAEELVRGIIKKFSDKEPLLKTLDKYVRVMRTEHCFLLFEELGKRDKWLQCLEVFRWM 120

Query: 121 QKQRWYIADNGVYSKLISIMGKKGQIRMAMWLFSEMRNSGCRPDTSVYNALITAHLHSKD 180
           QKQRWYIADNGVYSKLISIMGKKGQIRMAMWLFSEMRNSGCRPDTSVYNALITAHLHSKD
Sbjct: 121 QKQRWYIADNGVYSKLISIMGKKGQIRMAMWLFSEMRNSGCRPDTSVYNALITAHLHSKD 180

Query: 181 KAKALVKVLWYFEKMKGMERCKPNIVTYNILTRAFAQAGKVEQVDALFKDLDESIVSADI 240
           KAKALVKVLWYFEKMKGMERCKPNIVTYNILTRAFAQA KV+QV+ LFKDLDES+VSADI
Sbjct: 181 KAKALVKVLWYFEKMKGMERCKPNIVTYNILTRAFAQAAKVDQVNTLFKDLDESVVSADI 240

Query: 241 YTYNGVMDAYGKIGMIKEMELMLSRMKSNEIKPDIITFNLLIDSYGKKQLFDKMEQVFKS 300
           YTYNGVMDAYGK G IKEMELML+RMKSN+IKPDII+FNLLIDSYGKKQLFDKMEQVFKS
Sbjct: 241 YTYNGVMDAYGKNGNIKEMELMLARMKSNQIKPDIISFNLLIDSYGKKQLFDKMEQVFKS 300

Query: 301 LLRSKERPTLPTFNSMITNYGKARLREKAEEVFRKMKGMGFDPSYITCESLIMMYGHCDC 360
           LLRSKERPTLPTFNSMITNYGKARLR+KAEEVFRKMK MG+DPSY+TCESLIMMYGHCDC
Sbjct: 301 LLRSKERPTLPTFNSMITNYGKARLRDKAEEVFRKMKDMGYDPSYVTCESLIMMYGHCDC 360

Query: 361 VSKAREIFDGMVTSGKEVRVSTLNSMLDVYCMNGLPMEADLLFESVSSMKVFPDATTYKL 420
           VSKAREIFDGMV SGKEVRVSTLN+MLDVYC+NGLP EAD+LFES  +MKVFPD+TTYKL
Sbjct: 361 VSKAREIFDGMVNSGKEVRVSTLNAMLDVYCINGLPFEADMLFESAGNMKVFPDSTTYKL 420

Query: 421 LYKAYTKADMKELLEKLLKNMDKAGIIPNKRFFLDALGTVGSSPEKPASARNRTGSRNSE 480
           LYKAYTKADMKELLEKLLKNMDKAGIIPNKRFFLDALGT+GSS EKP  AR +TGSRNSE
Sbjct: 421 LYKAYTKADMKELLEKLLKNMDKAGIIPNKRFFLDALGTIGSSREKPEPARTKTGSRNSE 480

Query: 481 SSVEKPASASARTRTGSRNSESSVEKPASSSARTRTGSRNSESSVEKPA 529
           SSVEK   +S+RTR+G RN ES+V+KP SS  RTRTGSRN ES VE  A
Sbjct: 481 SSVEK--QSSSRTRSGPRNLESNVQKPISS--RTRTGSRNLESGVENHA 525

BLAST of Spg012914 vs. ExPASy TrEMBL
Match: A0A1S3B2D9 (pentatricopeptide repeat-containing protein At4g39620, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103485009 PE=3 SV=1)

HSP 1 Score: 885.6 bits (2287), Expect = 1.1e-253
Identity = 458/529 (86.58%), Postives = 489/529 (92.44%), Query Frame = 0

Query: 1   MPLSFSLQFASTFTLS-SSSPNSQLSRPWLPLPSSLRRDAATRVVCISTRPKRKSGAKTN 60
           MPLS S QF+  FT S SSS  SQLSR WLP+PSSL++  +TRVVC+STR  RKSG KT+
Sbjct: 1   MPLSLSPQFSPIFTTSYSSSRTSQLSRLWLPVPSSLKKTFSTRVVCVSTRLSRKSGVKTD 60

Query: 61  RSEAEELVRSVVRKFSDKEPLLKTLDKYVRVMRTEHCFLLFEELGKRDKWLQCLEVFRWM 120
           RSEAEELVR +++KFSDKEPLLKTLDKYVRVMRTEHCFLLFEELGKRDKWLQCLEVFRWM
Sbjct: 61  RSEAEELVRGIIKKFSDKEPLLKTLDKYVRVMRTEHCFLLFEELGKRDKWLQCLEVFRWM 120

Query: 121 QKQRWYIADNGVYSKLISIMGKKGQIRMAMWLFSEMRNSGCRPDTSVYNALITAHLHSKD 180
           QKQRWYIADNGVYSKLISIMGKKGQIRMAMWLFSEMRNSGCRPDTSVYNALITAHLHSKD
Sbjct: 121 QKQRWYIADNGVYSKLISIMGKKGQIRMAMWLFSEMRNSGCRPDTSVYNALITAHLHSKD 180

Query: 181 KAKALVKVLWYFEKMKGMERCKPNIVTYNILTRAFAQAGKVEQVDALFKDLDESIVSADI 240
           KAKALVKVLWYFEKMKGMERCKPNIVTYNILTRAFAQA KV+QV+ LFKDLDES+VSADI
Sbjct: 181 KAKALVKVLWYFEKMKGMERCKPNIVTYNILTRAFAQAAKVDQVNTLFKDLDESVVSADI 240

Query: 241 YTYNGVMDAYGKIGMIKEMELMLSRMKSNEIKPDIITFNLLIDSYGKKQLFDKMEQVFKS 300
           YTYNGVMDAYGK G IKEMELML+RMKSN+IKPDII+FNLLIDSYGKKQLFDKMEQVFKS
Sbjct: 241 YTYNGVMDAYGKNGNIKEMELMLARMKSNQIKPDIISFNLLIDSYGKKQLFDKMEQVFKS 300

Query: 301 LLRSKERPTLPTFNSMITNYGKARLREKAEEVFRKMKGMGFDPSYITCESLIMMYGHCDC 360
           LLRSKERPTLPTFNSMITNYGKARLR+KAEEVFRKMK MG+DPSY+TCESLIMMYGHCDC
Sbjct: 301 LLRSKERPTLPTFNSMITNYGKARLRDKAEEVFRKMKDMGYDPSYVTCESLIMMYGHCDC 360

Query: 361 VSKAREIFDGMVTSGKEVRVSTLNSMLDVYCMNGLPMEADLLFESVSSMKVFPDATTYKL 420
           VSKAREIFDGMV SGKEVRVSTLN+MLDVYC+NGLP EAD+LFES  +MKVFPD+TTYKL
Sbjct: 361 VSKAREIFDGMVNSGKEVRVSTLNAMLDVYCINGLPFEADMLFESAGNMKVFPDSTTYKL 420

Query: 421 LYKAYTKADMKELLEKLLKNMDKAGIIPNKRFFLDALGTVGSSPEKPASARNRTGSRNSE 480
           LYKAYTKADMKELLEKLLKNMDKAGIIPNKRFFLDALGT+GSS EKP  AR +TGSRNSE
Sbjct: 421 LYKAYTKADMKELLEKLLKNMDKAGIIPNKRFFLDALGTIGSSREKPEPARTKTGSRNSE 480

Query: 481 SSVEKPASASARTRTGSRNSESSVEKPASSSARTRTGSRNSESSVEKPA 529
           SSVEK   +S+RTR+G RN ES+V+KP SS  RTRTGSRN ES VE  A
Sbjct: 481 SSVEK--QSSSRTRSGPRNLESNVQKPISS--RTRTGSRNLESGVENHA 525

BLAST of Spg012914 vs. ExPASy TrEMBL
Match: A0A6J1IMR8 (pentatricopeptide repeat-containing protein At4g39620, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111478441 PE=3 SV=1)

HSP 1 Score: 883.6 bits (2282), Expect = 4.1e-253
Identity = 453/505 (89.70%), Postives = 475/505 (94.06%), Query Frame = 0

Query: 1   MPLSFSLQFASTFT-LSSSSPNSQLSRPWLPLPSSLRRDAATRVVCISTRPKRKSGAKTN 60
           MPLSFSLQF+ +FT  SSSS N Q S+ WLPLP SLRR  +TRVVCISTRPKRKSGAKT+
Sbjct: 1   MPLSFSLQFSPSFTPFSSSSRNFQRSQTWLPLPLSLRRAVSTRVVCISTRPKRKSGAKTD 60

Query: 61  RSEAEELVRSVVRKFSDKEPLLKTLDKYVRVMRTEHCFLLFEELGKRDKWLQCLEVFRWM 120
           RSEAEELVR V+RKFSDKEPLLKTLDKYVRVMRTEHCFLLFEELGK+DKWLQCLEVFRWM
Sbjct: 61  RSEAEELVRGVIRKFSDKEPLLKTLDKYVRVMRTEHCFLLFEELGKKDKWLQCLEVFRWM 120

Query: 121 QKQRWYIADNGVYSKLISIMGKKGQIRMAMWLFSEMRNSGCRPDTSVYNALITAHLHSKD 180
           QKQRWYIADNGVYSKLISIMGKKGQIRMAMWLFSEMRNSGCRPDTSVYNALITAHLHSKD
Sbjct: 121 QKQRWYIADNGVYSKLISIMGKKGQIRMAMWLFSEMRNSGCRPDTSVYNALITAHLHSKD 180

Query: 181 KAKALVKVLWYFEKMKGMERCKPNIVTYNILTRAFAQAGKVEQVDALFKDLDESIVSADI 240
           KAKALVKVLWYFEKMKGMERCKPN+VTYNILTRAFAQAGKV+QV+ALFKDLDESIVSADI
Sbjct: 181 KAKALVKVLWYFEKMKGMERCKPNVVTYNILTRAFAQAGKVDQVNALFKDLDESIVSADI 240

Query: 241 YTYNGVMDAYGKIGMIKEMELMLSRMKSNEIKPDIITFNLLIDSYGKKQLFDKMEQVFKS 300
           YTYNGVMDAYGK GMIKEME ML+RMKSNEIKPDII+FNLLIDSYGKKQLFDKMEQVFKS
Sbjct: 241 YTYNGVMDAYGKNGMIKEMETMLARMKSNEIKPDIISFNLLIDSYGKKQLFDKMEQVFKS 300

Query: 301 LLRSKERPTLPTFNSMITNYGKARLREKAEEVFRKMKGMGFDPSYITCESLIMMYGHCDC 360
           LLRSKERPTLPTFNSMITNYGKARLRE+AEEVFRKMKGMG+DPSY+TCESLIMMYGHCDC
Sbjct: 301 LLRSKERPTLPTFNSMITNYGKARLRERAEEVFRKMKGMGYDPSYVTCESLIMMYGHCDC 360

Query: 361 VSKAREIFDGMVTSGKEVRVSTLNSMLDVYCMNGLPMEADLLFESVSSMKVFPDATTYKL 420
           VSKAREIFDGMV SGK VRVSTLN+MLDVYCMNGLPMEADLLFES SSM+VFPD+TTYKL
Sbjct: 361 VSKAREIFDGMVNSGKAVRVSTLNAMLDVYCMNGLPMEADLLFESASSMRVFPDSTTYKL 420

Query: 421 LYKAYTKADMKELLEKLLKNMDKAGIIPNKRFFLDALGTVGSSPEKPASARNRTGSRNSE 480
           LYKAYTKAD KEL+EKLLKNMDKAGIIPNKRFFLDALGTVGSSPEKP  A   TGSR+SE
Sbjct: 421 LYKAYTKADAKELVEKLLKNMDKAGIIPNKRFFLDALGTVGSSPEKPEPASTGTGSRHSE 480

Query: 481 SSVEKPASASARTRTGSRNSESSVE 505
           SSVE P   S+R R GSR  ESSV+
Sbjct: 481 SSVENP--ISSRNRRGSRKLESSVK 503

BLAST of Spg012914 vs. TAIR 10
Match: AT4G39620.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 602.8 bits (1553), Expect = 2.7e-172
Identity = 318/491 (64.77%), Postives = 385/491 (78.41%), Query Frame = 0

Query: 18  SSPNSQLSRPWLPLPSSL---RRDAATRVVC--ISTRPK---RKSGAKTNRSEAEELVRS 77
           SS   +    WL    +L   RR   TR+ C  IS+R K   R+S  + NR     LVRS
Sbjct: 18  SSIPKETDHKWLRFSVNLGDARRSTRTRITCGAISSRRKLAERESAERENR----VLVRS 77

Query: 78  VVRKFSDKEPLLKTLDKYVRVMRTEHCFLLFEELGKRDKWLQCLEVFRWMQKQRWYIADN 137
           ++ + SD+EPL+KTLDKYV+V+R +HCFLLFEELGK DKWLQCLEVFRWMQKQRWYI DN
Sbjct: 78  LMSRISDREPLVKTLDKYVKVVRCDHCFLLFEELGKSDKWLQCLEVFRWMQKQRWYIPDN 137

Query: 138 GVYSKLISIMGKKGQIRMAMWLFSEMRNSGCRPDTSVYNALITAHLHSKDKAKALVKVLW 197
           GVYSKLIS+MGKKGQ RMAMWLFSEM+NSGCRPD SVYNALITAHLH++DKAKAL KV  
Sbjct: 138 GVYSKLISVMGKKGQTRMAMWLFSEMKNSGCRPDASVYNALITAHLHTRDKAKALEKVRG 197

Query: 198 YFEKMKGMERCKPNIVTYNILTRAFAQAGKVEQVDALFKDLDESIVSADIYTYNGVMDAY 257
           Y +KMKG+ERC+PN+VTYNIL RAFAQ+GKV+QV+ALFKDLD S VS D+YT+NGVMDAY
Sbjct: 198 YLDKMKGIERCQPNVVTYNILLRAFAQSGKVDQVNALFKDLDMSPVSPDVYTFNGVMDAY 257

Query: 258 GKIGMIKEMELMLSRMKSNEIKPDIITFNLLIDSYGKKQLFDKMEQVFKSLLRSKERPTL 317
           GK GMIKEME +L+RM+SNE KPDIITFN+LIDSYGKKQ F+KMEQ FKSL+RSKE+PTL
Sbjct: 258 GKNGMIKEMEAVLTRMRSNECKPDIITFNVLIDSYGKKQEFEKMEQTFKSLMRSKEKPTL 317

Query: 318 PTFNSMITNYGKARLREKAEEVFRKMKGMGFDPSYITCESLIMMYGHCDCVSKAREIFDG 377
           PTFNSMI NYGKAR+ +KAE VF+KM  M + PS+IT E +IMMYG+C  VS+AREIF+ 
Sbjct: 318 PTFNSMIINYGKARMIDKAEWVFKKMNDMNYIPSFITYECMIMMYGYCGSVSRAREIFEE 377

Query: 378 MVTSGKEVRVSTLNSMLDVYCMNGLPMEADLLFESVSSMKVFPDATTYKLLYKAYTKADM 437
           +  S + ++ STLN+ML+VYC NGL +EAD LF + S+ +V PDA+TYK LYKAYTKADM
Sbjct: 378 VGESDRVLKASTLNAMLEVYCRNGLYIEADKLFHNASAFRVHPDASTYKFLYKAYTKADM 437

Query: 438 KELLEKLLKNMDKAGIIPNKRFFLDALGTVGSSPEKPASARNRTGSRNSESSVEKPASAS 497
           KE ++ L+K M+K GI+PNKRFFL+AL   GS    P S     GS N +S+    +  S
Sbjct: 438 KEQVQILMKKMEKDGIVPNKRFFLEALEVFGS--RLPGS-----GSENRKSTRSSRSRDS 497

Query: 498 ARTRTGSRNSE 501
            + R G++ +E
Sbjct: 498 PKGRGGNQLTE 497

BLAST of Spg012914 vs. TAIR 10
Match: AT4G39620.2 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 601.3 bits (1549), Expect = 7.9e-172
Identity = 318/499 (63.73%), Postives = 382/499 (76.55%), Query Frame = 0

Query: 18  SSPNSQLSRPWLPLPSSL---RRDAATRVVC--ISTRPK---RKSGAKTNRSEAEELVRS 77
           SS   +    WL    +L   RR   TR+ C  IS+R K   R+S  + NR     LVRS
Sbjct: 18  SSIPKETDHKWLRFSVNLGDARRSTRTRITCGAISSRRKLAERESAERENR----VLVRS 77

Query: 78  VVRKFSDKEPLLKTLDKYVRVMRTEHCFLLFEELGKRDKWLQCLEVFRWMQKQRWYIADN 137
           ++ + SD+EPL+KTLDKYV+V+R +HCFLLFEELGK DKWLQCLEVFRWMQKQRWYI DN
Sbjct: 78  LMSRISDREPLVKTLDKYVKVVRCDHCFLLFEELGKSDKWLQCLEVFRWMQKQRWYIPDN 137

Query: 138 GVYSKLISIMGKKGQIRMAMWLFSEMRNSGCRPDTSVYNALITAHLHSKDKAKALVKVLW 197
           GVYSKLIS+MGKKGQ RMAMWLFSEM+NSGCRPD SVYNALITAHLH++DKAKAL KV  
Sbjct: 138 GVYSKLISVMGKKGQTRMAMWLFSEMKNSGCRPDASVYNALITAHLHTRDKAKALEKVRG 197

Query: 198 YFEKMKGMERCKPNIVTYNILTRAFAQAGKVEQVDALFKDLDESIVSADIYTYNGVMDAY 257
           Y +KMKG+ERC+PN+VTYNIL RAFAQ+GKV+QV+ALFKDLD S VS D+YT+NGVMDAY
Sbjct: 198 YLDKMKGIERCQPNVVTYNILLRAFAQSGKVDQVNALFKDLDMSPVSPDVYTFNGVMDAY 257

Query: 258 GKIGMIKEMELMLSRMKSNEIKPDIITFNLLIDSYGKKQLFDKMEQVFKSLLRSKERPTL 317
           GK GMIKEME +L+RM+SNE KPDIITFN+LIDSYGKKQ F+KMEQ FKSL+RSKE+PTL
Sbjct: 258 GKNGMIKEMEAVLTRMRSNECKPDIITFNVLIDSYGKKQEFEKMEQTFKSLMRSKEKPTL 317

Query: 318 PTFNSMITNYGKARLREKAEEVFRKMKGMGFDPSYITCESLIMMYGHCDCVSKAREIFDG 377
           PTFNSMI NYGKAR+ +KAE VF+KM  M + PS+IT E +IMMYG+C  VS+AREIF+ 
Sbjct: 318 PTFNSMIINYGKARMIDKAEWVFKKMNDMNYIPSFITYECMIMMYGYCGSVSRAREIFEE 377

Query: 378 MVTSGKEVRVSTLNSMLDVYCMNGLPMEADLLFESVSSMKVFPDATTYKLLYKAYTKADM 437
           +  S + ++ STLN+ML+VYC NGL +EAD LF + S+ +V PDA+TYK LYKAYTKADM
Sbjct: 378 VGESDRVLKASTLNAMLEVYCRNGLYIEADKLFHNASAFRVHPDASTYKFLYKAYTKADM 437

Query: 438 KELLEKLLKNMDKAGIIPNKRFFLDALGTVGSSPEKPASARNRTGSRNSESSVEKPASAS 497
           KE ++ L+K M+K GI+PNKRFFL+AL   GS    P S      S  S  S + P    
Sbjct: 438 KEQVQILMKKMEKDGIVPNKRFFLEALEVFGS--RLPGSGSENRKSTRSSRSRDSPKGRG 497

Query: 498 ARTRTGSRNSESSVEKPAS 509
               T  ++ + S   P S
Sbjct: 498 GNQLTEFQDKDLSSPFPES 510

BLAST of Spg012914 vs. TAIR 10
Match: AT3G06430.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 167.2 bits (422), Expect = 3.8e-41
Identity = 101/354 (28.53%), Postives = 174/354 (49.15%), Query Frame = 0

Query: 102 ELGKRDKWLQCLEVFRWMQKQRWYIADNGVYSKLISIMGKKGQIRMAMWLFSEMRNSGCR 161
           +L  + +WLQ LEVF  +++Q +Y    G Y KL+ ++GK GQ   A  LF EM   G  
Sbjct: 97  DLIAKKQWLQALEVFDMLREQTFYQPKEGTYMKLLVLLGKSGQPNRAQKLFDEMLEEGLE 156

Query: 162 PDTSVYNALITAHLHSK--DKAKALVKVLWYFEKMKGMERCKPNIVTYNILTRAFAQAGK 221
           P   +Y AL+ A+  S   D A +++      +KMK   +C+P++ TY+ L +A   A +
Sbjct: 157 PTVELYTALLAAYTRSNLIDDAFSIL------DKMKSFPQCQPDVFTYSTLLKACVDASQ 216

Query: 222 VEQVDALFKDLDESIVSADIYTYNGVMDAYGKIGMIKEMELMLSRM-KSNEIKPDIITFN 281
            + VD+L+K++DE +++ +  T N V+  YG++G   +ME +LS M  S   KPD+ T N
Sbjct: 217 FDLVDSLYKEMDERLITPNTVTQNIVLSGYGRVGRFDQMEKVLSDMLVSTACKPDVWTMN 276

Query: 282 LLIDSYGKKQLFDKMEQVFKSLLRSKERPTLPTFNSMITNYGKARLREKAEEVFRKMKGM 341
           +++  +G     D ME  ++        P   TFN +I +YGK R+ +K   V   M+ +
Sbjct: 277 IILSVFGNMGKIDMMESWYEKFRNFGIEPETRTFNILIGSYGKKRMYDKMSSVMEYMRKL 336

Query: 342 GFDPSYITCESLIMMYGHCDCVSKAREIFDGMVTSGKEVRVSTLNSMLDVYCMNGLPMEA 401
            F  +  T  ++I  +            FD M + G +    T   +++ Y   GL  + 
Sbjct: 337 EFPWTTSTYNNIIEAFADVGDAKNMELTFDQMRSEGMKADTKTFCCLINGYANAGLFHKV 396

Query: 402 DLLFESVSSMKVFPDATTYKLLYKAYTKADMKELLEKLLKNMDKAGIIPNKRFF 453
               +  +  ++  +   Y  +  A  KAD    +E++   M +   + + R F
Sbjct: 397 ISSVQLAAKFEIPENTAFYNAVISACAKADDLIEMERVYIRMKERQCVCDSRTF 444

BLAST of Spg012914 vs. TAIR 10
Match: AT3G53170.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 161.8 bits (408), Expect = 1.6e-39
Identity = 108/381 (28.35%), Postives = 173/381 (45.41%), Query Frame = 0

Query: 101 EELGKRDKWLQCLEVFRWMQKQRWYIADNGVYSKLISIMGKKGQIRMAMWLFSEMRNSGC 160
           +E  K ++W   L++F  ++KQ WY      Y+KL  ++G   Q   A  LF  M + G 
Sbjct: 116 DEAIKENRWQSALKIFNLLRKQHWYEPRCKTYTKLFKVLGNCKQPDQASLLFEVMLSEGL 175

Query: 161 RPDTSVYNALITAHLHSKDKAKALVKVLWYFEKMKGMERCKPNIVTYNILTRAFAQAGKV 220
           +P   VY +LI+ +     K++ L K     E MK +  CKP++ T+ +L     + G+ 
Sbjct: 176 KPTIDVYTSLISVY----GKSELLDKAFSTLEYMKSVSDCKPDVFTFTVLISCCCKLGRF 235

Query: 221 EQVDALFKDLDESIVSADIYTYNGVMDAYGKIGMIKEMELML------------------ 280
           + V ++  ++    V     TYN ++D YGK GM +EME +L                  
Sbjct: 236 DLVKSIVLEMSYLGVGCSTVTYNTIIDGYGKAGMFEEMESVLADMIEDGDSLPDVCTLNS 295

Query: 281 ------------------SRMKSNEIKPDIITFNLLIDSYGKKQLFDKMEQVFKSLLRSK 340
                             SR +   ++PDI TFN+LI S+GK  ++ KM  V   + +  
Sbjct: 296 IIGSYGNGRNMRKMESWYSRFQLMGVQPDITTFNILILSFGKAGMYKKMCSVMDFMEKRF 355

Query: 341 ERPTLPTFNSMITNYGKARLREKAEEVFRKMKGMGFDPSYITCESLIMMYGHCDCVSKAR 400
              T  T+N +I  +GKA   EK ++VFRKMK  G  P+ IT  SL+  Y     V K  
Sbjct: 356 FSLTTVTYNIVIETFGKAGRIEKMDDVFRKMKYQGVKPNSITYCSLVNAYSKAGLVVKID 415

Query: 401 EIFDGMVTSGKEVRVSTLNSMLDVYCMNGLPMEADLLFESVSSMKVFPDATTYKLLYKAY 446
            +   +V S   +     N +++ Y   G       L+  +   K  PD  T+  + K Y
Sbjct: 416 SVLRQIVNSDVVLDTPFFNCIINAYGQAGDLATMKELYIQMEERKCKPDKITFATMIKTY 475

BLAST of Spg012914 vs. TAIR 10
Match: AT5G48730.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 156.0 bits (393), Expect = 8.8e-38
Identity = 111/386 (28.76%), Postives = 183/386 (47.41%), Query Frame = 0

Query: 108 KWLQCLEVFRWMQKQRWYIADNGVYSKLISIMGKKGQIRMAMWLFSEMRNSGCRPDTSVY 167
           +W   ++VF  +++Q WY  + G+Y KLI ++GK  Q   A  LF EM N GC  +  VY
Sbjct: 129 RWESAIQVFELLREQLWYKPNVGIYVKLIVMLGKCKQPEKAHELFQEMINEGCVVNHEVY 188

Query: 168 NALITAHLHSK--DKAKALVKVLWYFEKMKGMERCKPNIVTYNILTRAFAQAGKVEQVDA 227
            AL++A+  S   D A  L+      E+MK    C+P++ TY+IL ++F Q    ++V  
Sbjct: 189 TALVSAYSRSGRFDAAFTLL------ERMKSSHNCQPDVHTYSILIKSFLQVFAFDKVQD 248

Query: 228 LFKDLDESIVSADIYTYNGVMDAYGKIGMIKEMELMLSRM-------------------- 287
           L  D+    +  +  TYN ++DAYGK  M  EME  L +M                    
Sbjct: 249 LLSDMRRQGIRPNTITYNTLIDAYGKAKMFVEMESTLIQMLGEDDCKPDSWTMNSTLRAF 308

Query: 288 ----------------KSNEIKPDIITFNLLIDSYGKKQLFDKMEQVFKSLLRSKERPTL 347
                           +S+ I+P+I TFN+L+DSYGK   + KM  V + + +     T+
Sbjct: 309 GGNGQIEMMENCYEKFQSSGIEPNIRTFNILLDSYGKSGNYKKMSAVMEYMQKYHYSWTI 368

Query: 348 PTFNSMITNYGKARLREKAEEVFRKMKGMGFDPSYITCESLIMMYGHCDCVSKAREIFDG 407
            T+N +I  +G+A   ++ E +FR M+     PS +T  SL+  YG      K   +   
Sbjct: 369 VTYNVVIDAFGRAGDLKQMEYLFRLMQSERIFPSCVTLCSLVRAYGRASKADKIGGVLRF 428

Query: 408 MVTSGKEVRVSTLNSMLDVYCMNGLPMEADLLFESVSSMKVFPDATTYKLLYKAYTKADM 453
           +  S   + +   N ++D Y       E   + E +      PD  TY+ + KAY  + M
Sbjct: 429 IENSDIRLDLVFFNCLVDAYGRMEKFAEMKGVLELMEKKGFKPDKITYRTMVKAYRISGM 488

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0036266.14.5e-25486.58pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa][more]
XP_004143565.15.9e-25487.12pentatricopeptide repeat-containing protein PPR5 homolog, chloroplastic [Cucumis... [more]
XP_008440667.12.2e-25386.58PREDICTED: pentatricopeptide repeat-containing protein At4g39620, chloroplastic ... [more]
XP_023543919.12.9e-25389.57pentatricopeptide repeat-containing protein At4g39620, chloroplastic [Cucurbita ... [more]
KAG7034169.16.5e-25389.57Pentatricopeptide repeat-containing protein, chloroplastic [Cucurbita argyrosper... [more]
Match NameE-valueIdentityDescription
Q9SV963.8e-17164.77Pentatricopeptide repeat-containing protein At4g39620, chloroplastic OS=Arabidop... [more]
A7LN871.9e-17063.88Pentatricopeptide repeat-containing protein PPR5, chloroplastic OS=Zea mays OX=4... [more]
A3ABE12.1e-16964.52Pentatricopeptide repeat-containing protein PPR5 homolog, chloroplastic OS=Oryza... [more]
Q9SQU65.4e-4028.53Pentatricopeptide repeat-containing protein At3g06430, chloroplastic OS=Arabidop... [more]
Q9SCP41.0e-3828.42Pentatricopeptide repeat-containing protein At3g53170 OS=Arabidopsis thaliana OX... [more]
Match NameE-valueIdentityDescription
A0A5A7T2662.2e-25486.58Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=11946... [more]
A0A0A0KGV82.9e-25487.12Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G501290 PE=3 SV=1[more]
A0A5D3CMK01.1e-25386.58Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3B2D91.1e-25386.58pentatricopeptide repeat-containing protein At4g39620, chloroplastic OS=Cucumis ... [more]
A0A6J1IMR84.1e-25389.70pentatricopeptide repeat-containing protein At4g39620, chloroplastic OS=Cucurbit... [more]
Match NameE-valueIdentityDescription
AT4G39620.12.7e-17264.77Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT4G39620.27.9e-17263.73Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT3G06430.13.8e-4128.53Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT3G53170.11.6e-3928.35Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT5G48730.18.8e-3828.76Pentatricopeptide repeat (PPR) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 233..304
e-value: 6.8E-16
score: 60.3
coord: 57..232
e-value: 2.4E-37
score: 130.3
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 305..527
e-value: 1.9E-30
score: 108.3
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 311..340
e-value: 2.3E-6
score: 27.5
coord: 346..374
e-value: 0.022
score: 15.0
coord: 381..404
e-value: 0.0012
score: 19.0
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 131..164
e-value: 2.9E-5
score: 21.9
coord: 205..236
e-value: 5.2E-4
score: 18.0
coord: 311..343
e-value: 3.6E-7
score: 27.9
coord: 275..308
e-value: 7.0E-6
score: 23.9
coord: 381..414
e-value: 3.6E-4
score: 18.5
coord: 240..274
e-value: 4.4E-8
score: 30.8
IPR002885Pentatricopeptide repeatPFAMPF13812PPR_3coord: 151..214
e-value: 3.3E-9
score: 36.7
coord: 226..286
e-value: 7.1E-9
score: 35.6
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 128..162
score: 11.443655
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 273..307
score: 10.413293
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 378..412
score: 8.801982
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 203..237
score: 9.306201
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 413..447
score: 9.174665
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 308..342
score: 11.39981
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 238..272
score: 11.432693
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 464..539
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 460..550
NoneNo IPR availablePANTHERPTHR47874:SF3BNAA01G05620D PROTEINcoord: 35..476
IPR044179Pentatricopeptide repeat-containing protein PPR5-likePANTHERPTHR47874EXPRESSED PROTEINcoord: 35..476

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg012914.1Spg012914.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003729 mRNA binding
molecular_function GO:0005515 protein binding